LOCUS       DAOSCC010000001        44659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_631_length_44659_cov_5.040691, whole genome shotgun sequence.
ACCESSION   DAOSCC010000001 DAOSCC010000000
VERSION     DAOSCC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 44659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44659
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_44659_cov_5.040691"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="mnmG"
                     /locus_tag="PLG72_00005"
     CDS             complement(<1..1472)
                     /gene="mnmG"
                     /locus_tag="PLG72_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOJ79220.1"
                     /translation="MEYVAGYFDVAVVGAGHAGCEAALACARLGMKTIVFSINLDAIA
                     NMPCNPSIGGTGKGHLVRELDALGGEMAKCADKTLIQSKVLNRAKGPAVYSLRAQVDR
                     KAYQMAMKNTLESQENLYIKQAEVTELLTNEDNTAVTGVKTLTGAVYYSRAVILTTGT
                     YLKGKIFIGDVSYSGGPDGLFPANKLSDSLREKGIRLMRFKTGTPARLNRKTIDFSKM
                     DPQPGDDVIIPFSFEDESIERDQALCWLTYTNNNTHEIIKKNLHRSPLYGGLIEGIGP
                     RYCPSIEDKVVRFADKERHQVFVEPMGLNTQEMYLQGMSSSLPEDVQTEMIKTMAGLE
                     NVHIMRSAYAIEYDCIDPTQLKLSLEFKNIRGLFSAGQINGSSGYEEAAAQGIIAGIN
                     AAQFIKQQPPLILDRSEAYIGVLIDDLVTKGTSEPYRIMTSRAEYRLLLRQDNADMRL
                     TEKGYKIGLISEERYRKFLDKKQRIEAEIERLENTYVPPS"
     gene            complement(1590..3014)
                     /gene="mnmE"
                     /locus_tag="PLG72_00010"
     CDS             complement(1590..3014)
                     /gene="mnmE"
                     /locus_tag="PLG72_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOJ79221.1"
                     /translation="MRDRSDSARAMPGRNAETIAAISTPYGTGGIGIIRISGPEAFSV
                     AEKIFRGRRKISETRSHSVVHGRIVDPADGQMVDEVLLVKMDAPGTFTGEDTIEINCH
                     GGIVVLRRVLSLVLRYGARAACPGEFTKRAFINGRMDLAQAEAVIDLINSKTDEGSRA
                     AAAQLQGRLSEKIRGARRKMIELIARIEAAIEYPEEDIEEITGEAVRNGIKEVREELR
                     KTAQSFTRGRLLRDGLTAAIIGKPNAGKSSLLNALVGSSRAIVTDIPGTTRDIIEEYV
                     NIKGIPVRFLDTAGIRSTDDPVEAIGVEKARTAAAEAELVIIVLDAQTGITDEDREIM
                     KTSLSKKTIVMINKTDVADDRMVEDMKEQIRTYGDIPVVVASMVDGTGMDDLADRIES
                     LAAGGEISANNEVLLTNARHRQLIEDAVLGLDSAESAYKGGMPLDLVTIDIRDAAEAL
                     GRITGESVSEDVVNEIFSRFCVGK"
     gene            complement(3270..3905)
                     /gene="jag"
                     /locus_tag="PLG72_00015"
     CDS             complement(3270..3905)
                     /gene="jag"
                     /locus_tag="PLG72_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003398827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOJ79222.1"
                     /translation="MSKVIETAGKTVDDAIEAALKQLGAEMDDVEVTVLEEGSKGLFG
                     IGSRQARVQVRLKPDPAEEGICFLESVFEKMNVDVDIEKYEDEDSVLFKISGKDSGII
                     IGRRGETLDALQYLTSLVVNKSSKKYMRVTIDIENYREKREETLARLARRLADKVVKY
                     RRNISLEPMNPYERRIIHSTLQNNEYVETYSVGEDPNRKVVITLKQAHSDR"
     gene            complement(3972..5093)
                     /locus_tag="PLG72_00020"
     CDS             complement(3972..5093)
                     /locus_tag="PLG72_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOJ79223.1"
                     /translation="MFDFLAYPLGKFLKFIYDITFENYGLAIIIFTFIVRLALLPLTV
                     KQQKSTMKMQELQPLINDIQKRYKDDREAMNQELMKLYQEHNYNPAGGCLPLLIQMPL
                     ILALYWVIVQPLKFMLGKSGEAIEAIVNAAAKAQAAVNASLGLENLPVTAENILRSWG
                     SHREIRALNFFNEHQDMLSEVSNFLDKSELIDFRSFLGLHLGEVASFRPHVVFGPEWK
                     IYLPLFILAIVATATTYLSSRLSMPKTNDQQGGASGCSANSMMYVGPVMTLMLSFQLP
                     AGVVLYWMTGYVFAIFQQLYINKFVLKKDKPAEAAASAGGKGSRTASKEESQEGNNGK
                     IDSGDDASAKQLPSGQNKGKANKAGGKKGGSKKGGKKKK"
     gene            complement(5225..5446)
                     /gene="yidD"
                     /locus_tag="PLG72_00025"
     CDS             complement(5225..5446)
                     /gene="yidD"
                     /locus_tag="PLG72_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ79224.1"
                     /translation="MGCIKKLLIAMIRFYQRYLSPLKPKGSCRFYPTCSQYAIDALSR
                     YGVLKGSYLAVRRILKCHPFHPGGYDPVK"
     gene            complement(5422..5790)
                     /gene="rnpA"
                     /locus_tag="PLG72_00030"
     CDS             complement(5422..5790)
                     /gene="rnpA"
                     /locus_tag="PLG72_00030"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ79225.1"
                     /translation="MKNTERLKKNYEFARIYRKGRFYPGKYLVLYLLNNGTEINRLGV
                     SVSKKIGKSVKRNRVKRLIRESYRHLEPYIPVGLDIVITARKVETEYRFAEILKEMKF
                     LFKKACAFNNKEKWDASRNS"
     gene            complement(5952..6086)
                     /gene="rpmH"
                     /locus_tag="PLG72_00035"
     CDS             complement(5952..6086)
                     /gene="rpmH"
                     /locus_tag="PLG72_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ79226.1"
                     /translation="MLRTFQPKKRQRSKEHGFRKRMSTKNGRKVLRRRRQKGRKVLSA
                     "
     gene            6522..7856
                     /gene="dnaA"
                     /locus_tag="PLG72_00040"
     CDS             6522..7856
                     /gene="dnaA"
                     /locus_tag="PLG72_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ79227.1"
                     /translation="MNLSLSGIWPRVLDLLKNELTEISFNTWINTIEPISFNSDTITL
                     GVPTDFNKGILESRYLTLISNAVRQVALKEYTIKITVPSQEPASKVKQQPETNVNEDI
                     YNSVLNPKYTFDTFVIGNGNRLAHAAAVAVAEAPAQAYNPLFLYGGVGLGKTHLMHAI
                     GHYILSQYPHMKVLYVSSEKFTNDLINAIKDDTNEEFRNKYRNMDVLLIDDIQFIGGK
                     ERTQEEFFHTFNALYEANKQIIISSDRPPKEFTTLEDRLRSRFEWGLTADIQSPDFET
                     RIAILKKKAQLENITIPDDVLAFIADKVVSNIRELEGALNRVIAYSSLTDTEISVDLC
                     AEALKEILAASNNKVIDTGHIIESVSRYFDIRMEEILSQKRNRNISYPRQIAMYLCRD
                     IMGLSLPKIGEEFGGRDHTTVIHAIQKIEEDIQLNPEIKRTVEELKRNITGK"
     gene            8088..9197
                     /gene="dnaN"
                     /locus_tag="PLG72_00045"
     CDS             8088..9197
                     /gene="dnaN"
                     /locus_tag="PLG72_00045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ79228.1"
                     /translation="MKIICSKEKLLEGINIVQKAVSAKTPLPILEGILVEAGDQVKLT
                     SNDLEIGIECIIEADVREEGAIVLNARMFGEIIRKFPDAEVLIEVRENNNVVIECENS
                     MFEIRGLDAEGFPALPTLEKKDGIKISQRMLKDMIRQTIFAVSTDENRPMLTGSLFEY
                     KDGKFTIVSIDGYRLAMRCFEPENENETVSAERKFVIPGKTLNEIAKILQPVDDDIYI
                     YAAGNQILFDMGNCIMTSRLLEGEYLNYMSIFPKDHETKVRVNTKDLLSCFERASLVI
                     TSEERRYPVSLDITEKKIVITANTEAGNVREELKIDMEGKRLEISFYHKFFIEALRAI
                     DNETIDIYFTTSLGPCTIKPLQGNDFAYLILPIRR"
     gene            9254..9463
                     /locus_tag="PLG72_00050"
     CDS             9254..9463
                     /locus_tag="PLG72_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOJ79229.1"
                     /translation="MKEIKISTEYIELEQLLKFAGLVNKGSDAKMLISSGAVLVNGQA
                     ELRRGKKLRPGDVVEYDGNEYMITE"
     gene            9488..10597
                     /gene="recF"
                     /locus_tag="PLG72_00055"
     CDS             9488..10597
                     /gene="recF"
                     /locus_tag="PLG72_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ79230.1"
                     /translation="MKVKSICLKNYRNYDNAEVEFADTFNIIYGNNAQGKTNIIEAIF
                     LCASGRSHRTPKDNELLKYGRDFFDIKLEYSRTDRDEQIHIVYSNEDRKRISINDIPL
                     KKIGGLMGHLNAVIFSPEDLLVIKQGPAERRRFIDITLSQLRPSYFFDLQQYAKILLQ
                     RNNLLKAISLNNDLADTLEVWDHHLVKTGARIMKARKDFIKRLDTIAAERHERLTNCE
                     EKLKLEYDPSFKIEEADEEKEVEDAFRRSIKKNREKELMKGTTLTGPQRDDIDIILNG
                     ESTRIYASQGQQRTSVLSMKLAEIDIMKEETGEYPVLLLDDVLSELDDRRKEYLLDSI
                     DEIQTLITSTDKRFFSRNSDGSAFFHVESGIIRRE"
     gene            10685..10966
                     /locus_tag="PLG72_00060"
     CDS             10685..10966
                     /locus_tag="PLG72_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOJ79231.1"
                     /translation="MFLHIGGDVVIPMKNVIAILDIETSTISKDTRDFLKTAEEEGFV
                     TAITDDLPRSFIITEEDKKSRIYLSPISSVTLQKRSGFMSDFMNDEYKL"
     gene            11002..12930
                     /gene="gyrB"
                     /locus_tag="PLG72_00065"
     CDS             11002..12930
                     /gene="gyrB"
                     /locus_tag="PLG72_00065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ79232.1"
                     /translation="MSGINTENHNKYDENQIQVLEGLEAVRKRPGMYIGSTSLRGLHH
                     LVYEIVANSIDEALAGRCDSIDIVVEKDNSVIVKDNGGGIPVGIHPKMGIPTVEVVHT
                     VLHAGGKFGGGAYTVSGGLHGVGASVVNALSEFLEVQVARDGKLYRQRYERGKTVTPL
                     EVIGEVDYTGTRTHFKPDPEIFEETEFDFEAMITRYREMAFLNKGIRITLTDNRGEEP
                     VKKEFHYEGGIVSYVEYINMGKDVLHDVPIYFDGEKDGSYCEIAMQYNDRYVESIFSY
                     ANNICTTEGGTHLTGFKTAITKVFNDYARKFNYLKENDKNLMGEDVREGLTAIVSVKL
                     INPQFEGQTKTKLGNSDIRNLVENVVTEKLMTYLEENPSVARAIIEKCIMASRAREAA
                     RKARELTRRKNALETTTLPGKLADCTERDPSLCEIYIVEGDSAGGSAKQGRDRRFQAI
                     LPLWGKMLNVEKSRLDKVIGNDKLMPVITALGAGIGEDFDLSKLRYDKVIIMADADVD
                     GSHIRTLLLTFFYRYMKPLVEKGHVYIALPPLYRVYKGKEEYYAYNEVELERIQKEKN
                     WKKEECNVQRYKGLGEMSSEQLWNTTMNPETRKMKRVTLEDAVAADKIFTILMGDKVE
                     PRKDFIQENAKYVTNLDI"
     gene            13350..14129
                     /locus_tag="PLG72_00070"
     CDS             13350..14129
                     /locus_tag="PLG72_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ79233.1"
                     /translation="MAKVIAIANQKGGVGKTTTAVNLSACLAYKGKKVMVIDVDPQGN
                     TTSGLGIDKQNIARSIYEVIINDESIEEALMPTMVENLFICPSNIQLVGAEVELVSVI
                     SRETRLKIALQDVRDNYDFIIIDCPPSLGLLTLNALTAADTILVPIQCEYYALEGLSQ
                     LMNTVKLVQRHLNPSLEVEGVVLTMFDARTNLSIQVVEDVKKYFRNKVYRTIIPRNVR
                     LSEAPSFGLPIILYDPKSKGAECYLDLAQEVIDYSEEVKDK"
     gene            14126..14977
                     /locus_tag="PLG72_00075"
     CDS             14126..14977
                     /locus_tag="PLG72_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ79234.1"
                     /translation="MKKGLGKGLGALLGTEEAENGGIMEIRISDIEPNVNQPRKTFDD
                     EKLAALAESIKQHGVVQPLIVQRDGDTYRIVAGERRWRAARLAGLDTVPVIVRDLSDR
                     QVMEVALIENLQREDLNPIEEAEAYERLISEFGMKQEEVASVVGKSRPAITNSIRLLS
                     LNDEIKSRVISGEISSGHARALLSLDDQKLQLEAMQEIIDKSLSVRETEKLIKQLMTP
                     KKQKEKKAPDAEYQAIEERFREIFGTKVRIMNNKKNGKILIEYYSLEELDRIINLVER
                     IYKNSKN"
     gene            15112..15675
                     /locus_tag="PLG72_00080"
     CDS             15112..15675
                     /locus_tag="PLG72_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOJ79235.1"
                     /translation="MKHSDEIKEFTEGEGSMSIFDLPYELIVFIGLNFLLTLILLFMN
                     ASNRSKLKRLKSKYDKFMNGLSGASMEDVLEDCIDKLNGVIEKNRELEYQINTIKRNM
                     HYCVQKVGVVRYNAFDDVGSDLCFSIALLDNNDDGVVISGLYARDSSTTYAKPISRGK
                     SKYVLSAEEIKAIDLARRSRVTDIYDD"
     gene            15778..17691
                     /locus_tag="PLG72_00085"
     CDS             15778..17691
                     /locus_tag="PLG72_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ79236.1"
                     /translation="MIKSGVISTDRTKCVGCNKCIRACIVPHANRVTDERKITVDYAN
                     CILCGECLKACNHGARSYVDDTREFFSELEKGTEVAVIVAPAFRFNYPDKFKNVLSWL
                     RTKGVRFIYDVSFGADITTYLYIKAIVEKKLNTVIAQPCPVIVNSIERYHTELIKYLS
                     PIGSPMYCTAVYLKKYDGFKGKIAAISPCIGKTDEFGRDDTIDYNVTFTKLMEAYNAA
                     GYDPGAETGFDSPESLVGYWYPTPGGLKESVEQIFGRKYHIRRIEGPKLAQEYLKEIS
                     QSPNVIPLVIDILNCSEGCLSGTGTEAGIPHDFKDRALFEKAGEENGKVLSNRKRQLK
                     KLVKHFDKTLKMEDFIYTYTMRKDDAVITEQDIESAFEALLKFNEEERTVDCAACGYE
                     SCHQMAEAVFLGYNFPDNCIHYFKKMLEKSHDEISLEKRKTEQLLAKANDMTDKQSEF
                     ISKLHSEVETVDQIIGELVQVYTNIVNDITNINEQMIDVESLSRSGVMSIDELNSRFD
                     QFFKMSESIRNISEQTHMLSLNASIEAARAGEYGKGFLVVAEEVRKLADDAKTAVAET
                     QDSNNVIEEDIRKINDLLSKLQSVVVVVNENIQNVLAASEEASASMHQIQDTIEKIVS
                     SAEEMSSAAGSEY"
     gene            17894..17984
                     /locus_tag="PLG72_00090"
     tRNA            17894..17984
                     /locus_tag="PLG72_00090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17929..17931,aa:Ser,seq:tga)
     gene            18082..18173
                     /locus_tag="PLG72_00095"
     tRNA            18082..18173
                     /locus_tag="PLG72_00095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18117..18119,aa:Ser,seq:gct)
     gene            18552..18743
                     /locus_tag="PLG72_00100"
     CDS             18552..18743
                     /locus_tag="PLG72_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79237.1"
                     /translation="MEDKIAYGRKLNLIGMIIFILAYLIDRFILNIPDLLYIIILLIV
                     MFLMFGGLLIRKRARDNEK"
     gene            18928..20505
                     /locus_tag="PLG72_00105"
     CDS             18928..20505
                     /locus_tag="PLG72_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOJ79238.1"
                     /translation="MAENIKNGKSDSHVPEQIPGRTCEAANEVQADERPVYAKEDKLF
                     LAGALLLGILFTWLFYGKNPGVSVPLFVIAFYALFFAYVRPAQDKKAMFGWFLCIPVL
                     LISMTFVLFRDLTLMVLNILALPCLIILQTVLITGTNNYRWYSPGIIVDLLFGFFARG
                     IVHAAKPFSMFASMVRSKTGGEKKSTVLRIITGLVIALPILLILLLILSSADMVFGKL
                     VEKLPDLLNSLNLGEVFIKTFLMLIIFICSFSYIWSLKHKEKFLDIDNGLLSAKSADE
                     KRRWDPVVLVTVTACIDLLYIFFVIIQFAYLFGRSGLPEGLTYSEYARNGFSELILVS
                     LLNMGWLALTLTYTKKGKRGLDTSFRILNTITVCCTYVMLLSAYYRMLMYEEAYGFTF
                     LRVMTQAFMIFLFVLFSTTLVRVWNDRIPLLKPYIAAAVIAFTVINYINVDAMVAKKN
                     VDRYFETGKIDIYYFNSLSNDAVAELVRLVDADDPEVAQKAAEILADRKERIYERTDW
                     QSFNLADLRAKRAISGK"
     gene            complement(20657..21148)
                     /locus_tag="PLG72_00110"
     CDS             complement(20657..21148)
                     /locus_tag="PLG72_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79239.1"
                     /translation="MQSKGSVLIFLAVLAFPIIALSTDHDIFFAVTAALVTLASIKSI
                     YNLIVLNGFEQIELDEDLEDDLEELIDIDVRKFGNGLNIIVHMIVIVFIIYCAFYLET
                     ILLKCVAALAILMQILFIIKKAQKNSAGFDKNKYKPQIFVSSISNIAIVLFTILNKLS
                     RLS"
     gene            complement(21410..23284)
                     /locus_tag="PLG72_00115"
     CDS             complement(21410..23284)
                     /locus_tag="PLG72_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ79240.1"
                     /translation="MKTEVAANLPANTGKKKSKLMIILLVFTGVMLASASVFAYITLR
                     YNNVYRGVHIGDLDVSGMARSEIIELLESRYTIPASGLDITLETDLAEVRKSYPDLGV
                     VYDIEAAADKAFSIGRSGNVFDRLYDIARASFKGIVTYVPQNYDETRIDDFVDEFALM
                     AFQQVRESILLISDKSVVIKSGRHGEAVDKAEVKSLVINMIKEGKGGVIRPDVIITET
                     TRFDAGEVYDKIVCDPENAYYKMENGNLVLVPHKTGRQIDREELKRIIDEHNKTENME
                     TTLPVTYVMPEITSEKAASMLFRDELARYSTSFETRTQNEKNRMHNIALAAGKYHNYI
                     LMPGEEFSFNEVVGPRNEKTGYKSAHVFVNGRVEDGIGGGICQATSTLYNAVLLADLE
                     VVERRNHSFIVTYVPLGQDATAYYGGTDLRFINNTGWPIKILSWVEGNKVNCVFLGTN
                     STPEKTVVISTKTLSHTPYKTKYVDDPALPVGTVKKSQYGTDGYVVETYKTVKMGDKV
                     ISQKKIHTSSYKPCDEEFLVGIKQPDGTLTPGLAAQMASKAKAAAQDETSTGDNQPAT
                     ADKPAADEKPGTEPVSGGTSGASSDSPETYGNAPVTPENSGDVPDTPEISDNDTVSME
                     "
     gene            23511..24101
                     /locus_tag="PLG72_00120"
     CDS             23511..24101
                     /locus_tag="PLG72_00120"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ79241.1"
                     /translation="MNVFNEAEFRDLCERLGAEGTIRDMTVRYPTPGYFNRVRNVIRH
                     DRRGEVVFCVIRPDGKAIMTRRNDYPENVYRIPTGGIGYGEDVLNAVFREVREELGLE
                     VRIRDFAGAVRIRFEHGNEHVMFYSYIFILDEIGGRLLADASDDEISEIKEVDLDEFE
                     QAVRALGSIGGKWQDWGKFRYITSNAVLNFLKHKSI"
     gene            24226..24453
                     /locus_tag="PLG72_00125"
     CDS             24226..24453
                     /locus_tag="PLG72_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HOJ79242.1"
                     /translation="MFRSSRAYAALTRDERQIIDAALEAREQWKEAGLNFNNVTDELL
                     VDYYIYWMKACESRYTYYLRLAKERGLSHYL"
     gene            24539..24808
                     /locus_tag="PLG72_00130"
     CDS             24539..24808
                     /locus_tag="PLG72_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOJ79243.1"
                     /translation="MNSGVILAYVAGFILVLVFARLFLKPLKVVMKLVLNTVLGALAL
                     LLANWIGGLFGFRIAFNVYTSFIVGVLGIPGFILLVILKLLFGTV"
     assembly_gap    24822..24921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25179..>27651
                     /gene="nifJ"
                     /locus_tag="PLG72_00135"
     CDS             25179..>27651
                     /gene="nifJ"
                     /locus_tag="PLG72_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ79244.1"
                     /translation="MGKNMKTMDGNTAAAYVAYAFTEVAGIYPITPSSVMAELVDEWS
                     ANGRKNIFGQPVRVVEMQSEAGAAGVVHGSLTAGALTTTFTASQGLLLMIPNLYKIAG
                     ELLPGVFHVSARAVASHALSIFGDHSDVMACRQTGAAMLAEGSVQEVMDLAPVAHLSA
                     IKGRVPFINFFDGFRTSHEIQKIEVIEYDDFAKLVDYDAVKEFRKRSLNPERPSVRGT
                     AQNPDIFFQARESSNVYYNELPAIVEEYMNKISALTGRKYGLFNYYGAPDADRIIIAM
                     GSVCETIEETIDYLMAKGEKVGVVKVHLYRPFSAKHLLKAIPETAKKIAVLDRTKEPG
                     ALGEPLYQDVCAALYENGKSPVIVAGRYGLGSKDTTPAQIVAVYDNLKSDNPKNHFTI
                     GIVDDVTNLSLPVGEEIDTTAEGTVSCKFWGFGSDGTVGANKNSIKIIGDHTDMYAQA
                     YFAYDSKKSGGVTISHLRFGKKPIRSTYLINKADFVACHNPAYVGKYDMVSDLKENGT
                     FLLNCGWSAEELNEKLPADMKRILAKRNIKLYTIDAVSIAKEIGLGSRINMIMQAAFF
                     KLANIIPLDDAVKYMKDAIVKSYGKKGEKVVNMNYLAVDKGIEALKKIDIPADWADAV
                     DEAAAAKDVPEFISKILEPMNRQEGDKLPVSAFKGREDGTFPCGTSRYEKRGVAVDVP
                     EWQMDKCIQCNQCSYVCPHAAIRPFLLDEEETGNAPEGFETKKAIGKGFEGLNFRMQV
                     SVLDCQGCGSCANVCPAKEKALIMKPLETQMDQVKNWDYAMTVKPKNPMGPSTVKGSQ
                     FEEPLLEFSGACAGCGETPYAKLITQ"
     assembly_gap    27652..27661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27662..28712
                     /locus_tag="PLG72_00140"
     CDS             <27662..28712
                     /locus_tag="PLG72_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ79245.1"
                     /translation="FGDRMYIANATGCSSIWGGSAPSMPYCTNKAGFGPSWANSLFED
                     NAEFGYGMYLAVKQIRNKIADNCRKLLEMEVPGDVRAAIEGWLDNMNDGDGSKAASAQ
                     LKAALDAYKGNAGDAAKVLVDEILENSEFLVKKSVWILGGDGWAYDIGYGGLDHVLAS
                     NEDINVLVFDTEVYSNTGGQSSKATPSGAVAQFAAAGKRTRKKDLGMMAMSYGYVYVA
                     QVAMGANQSQLIKALLEAEKYDGPSLIIAYAPCINHGLRLGMGQSQLEEKNAVEAGYW
                     HLYRYNPLLKEEGKNPFTLDSKEPTASYRDFIMGEVRYSALTRTFPDIAEQLFEQAEK
                     DAKERLEAYKRLANG"
     gene            29064..30053
                     /locus_tag="PLG72_00145"
     CDS             29064..30053
                     /locus_tag="PLG72_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HOJ79246.1"
                     /translation="MQGMENEIKQCFGIDVRSIFPYKDAYVAVTAAGKKLVKRVLLSP
                     ERLKFVHWAKEHLVSNGFEGVDRYLVTLAGEPGFWCNDCLYVMTDYVEGRESSFDDDE
                     DLKMAAEALAALHRASAGYLPPEGSKVRDDLGKLPVYFSKRLNDITKMRKQARKGKSR
                     FDHLFLQYADHFISMGENAIHRLSASSYDKIADRTRKERSFCHHDYTHHNILLNDGKV
                     TVTNFDYCCFELKAYDIANLIRRKMRRCGWDISKAGLITESYNRILPLGMDEMEIVRI
                     MLEFPQKFWRVVNRYYNSRRSWSEKVFLGRLEEVIEETGPLDAFLKAYDREFL"
     gene            complement(30074..31744)
                     /locus_tag="PLG72_00150"
     CDS             complement(30074..31744)
                     /locus_tag="PLG72_00150"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ79247.1"
                     /translation="MLTDIEIAQKCKMKPITEVASTLGINPDELELYGKYKAKLTDSL
                     WQRVRNNRDGKLILVTAVNPTPAGEGKTTTTVGLGQALTAMGKKAVICLREPSLGPVM
                     GMKGGAAGGGYAQLVPMEDINLHFTGDIHAVTAANNLLSAVIDNHIHQGNALRIDPRQ
                     IVWKRAMDMNDRALRNIVVGLGGKADGTPREDGFLITAASEIMAILCLAENITDLKNR
                     LGKIIVGYDYSGAPVTVSQLKAEGAMALLLKDAIKPNIVQTLEHSPALMHGGPFANIA
                     HGCNSIKATRLALKLADYVITEAGFGADLGAEKFFDIKCRFAGFVPDAAVLVTTVRAL
                     KYNGSVPKEKLAGEDLEALKRGFPNLEKHVENLRKFGVPVLVAINLFETDTPAELEYI
                     RKRCEDLNVAAAISQVYTKGGEGGRELAEKLLNLLDTVPSRFRLLYDVKAPVKEKIET
                     IAKEIYGARGVTYSPAADKTIKKLAEMKLDDLPVCMAKTQYSLSDNPKVLGRPENFEI
                     NVRDIRVSAGAGFIVALTGEIMTMPGLPKVPAAEQIDISEDGSITGLF"
     gene            31935..32831
                     /gene="yabG"
                     /locus_tag="PLG72_00155"
     CDS             31935..32831
                     /gene="yabG"
                     /locus_tag="PLG72_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOJ79248.1"
                     /translation="MSGIKIGDIVARKSYGCDVFFKVTDITERDGERILTLKGISYRI
                     EADAPESDLEVQPEVKVREYRDRCWREAEKTAADRIYDMRYYRYYMPRSRGWRKKAYY
                     RDTSNDKPDKIKRPGKVLHLDGDRDYLENCIAEYKKLGIDVVGEYVPEKDQPARVYSL
                     LSEHRPDILVLTGHDGFIKGKDSYNDISSYRTSKYFIEAVKEARRFNSDMDGLVIFAG
                     ACQSMYNEIIKAGANFASAPFRDLIHALDPVRVCQKIAFASIDTVLDAEDVINNTITG
                     KTGIGGIQTRGKYREGFPVEPY"
     gene            33334..33600
                     /locus_tag="PLG72_00160"
     CDS             33334..33600
                     /locus_tag="PLG72_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOJ79249.1"
                     /translation="MAEKKDLVQIKKNIETCIGKRVQLKANKGRKKAFIKEGIIENSY
                     PSIFVIKFENEYETTRRVSFSYTDILTKAVELVIYKDDKEILVS"
     gene            33788..35353
                     /locus_tag="PLG72_00165"
     CDS             33788..35353
                     /locus_tag="PLG72_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOJ79250.1"
                     /translation="MSLELVGEPIRINRTIGEDSTQTIVENDIIVPDTKPDIARILLL
                     DGDAWVEGAEAAADRVLVTGSVRYKILYISDDPEQPVKSITTVSTFQYAMEIPDTRQG
                     MQCRVKCDIEHLEYEILNSRKVNVKAIISLSGKVTDQVEQYITRDLTGSDGIQILRNR
                     AAVNAYLGDCRTDCRISETLDIPVGKPAIYEILRNDIKITGREYMAGDDKIAVRGNLN
                     ISTLYIADNENRSIQFMEHEIPFTRMIDMPGIDETCSTSLDITIGEASFEAEEDGDGE
                     LRRLNCDVMLDIYAQCYGRKEIELVEDAYSPYSRVNIEKEELLLDELVSENVTQLTLR
                     ENIEADENAPDISELFNVLGKLSLSSGQITDDRVIVEGVAVCNVLYLASSEGQPVYCT
                     EREIPFRQSIEVKGAREGMNLDVEMEIEHYSYSIVNSREVEVRLVIGLKTKVGKQLSV
                     PVIVNAQEQPLDEKRAEARPSIIIYFTQPGDTLWKIAKRYYTTVDEILKNNDLYETEQ
                     IAPGSRIIITKKL"
     gene            35527..36390
                     /gene="ispE"
                     /locus_tag="PLG72_00170"
     CDS             35527..36390
                     /gene="ispE"
                     /locus_tag="PLG72_00170"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ79251.1"
                     /translation="MSYIEIPAYAKINLSLDVIRRREDGYHELKMVMQTVSLHDTVCL
                     ETTGDAGIHLECSGGAVPCDSTNTAWRAAELMFERYGMAGGLRIWITKRIPAAAGLAG
                     GSADAAAVLRGINELYGLDLDAGELKRLGLQIGADVPYCIEGGTRLAEGIGEILTPLP
                     DFSGIDVVVVKPGISVSTPWVYSQLDLSKVTDNDRPDTGKLIGALASRDIKAASLYMK
                     NVLELVTVRRYEIVRYAKSRMAETGAEACLMSGSGPSVFGLFADQVKAQKAYAMLSEY
                     HEWECFIAKTV"
     gene            36437..37129
                     /locus_tag="PLG72_00175"
     CDS             36437..37129
                     /locus_tag="PLG72_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ79252.1"
                     /translation="MAGKLSKVNLNDYKPLRDVIFDTLREAIIVGELKPGQRLMEVQL
                     AEKMGVSRTPVREAIRKLELEGLVEMLPRKGAHVAELSVKDIMDVLEVRATLDGLATS
                     LSATRISEDEKKELRHILDQFINCVEKENLQGSIKKDVEFHDVIYKSSRNDKLIQISN
                     NLREQVQRFRVVYIKDYSSSRELIREHTEIYEAISAGNPEAAMRAAQRHIKNQEESII
                     RSLKKKHSEAEK"
     gene            complement(37149..37355)
                     /locus_tag="PLG72_00180"
     CDS             complement(37149..37355)
                     /locus_tag="PLG72_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOJ79253.1"
                     /translation="MEVVFTVPSITCSICSGRIRSELGRLEGVSGVDIDLKTQSVKVS
                     YDPGLQKPQDIRRKIAELGYEVVE"
     gene            37552..39183
                     /locus_tag="PLG72_00185"
     CDS             37552..39183
                     /locus_tag="PLG72_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ79254.1"
                     /translation="MPSGESWYDSFLSKYRAGISHEFLFYFNVRDLVDNYRYMDRYIY
                     EEFIKKRNFGIVAFYDISRGLTFLDAGMEKEFNIITSNEASNLMNAMPSKIFPFIDRA
                     LKNTKMALFIDHIEKIIPAGDIASMTLEERIALIWICEWSVSPKISSVGSCIMMLADN
                     LADVSRELLKSAYRIEPIFVGLPDEDQRKEYIEYLLRDGSVKTEITADEFAKLSSGLS
                     KKSIKDIKLKSEAEDIPISFEFIKEKKHSVLQKEYGDVLEFIYPEIGFEDIGGMEKAK
                     EYLLKNVVYPIRKGDLRRVPMGILLCGPSGTGKSLLVYALAKASGFNCVKIDMSRILG
                     QYVGESEKNFKKCLLGAQSQHPVIVFVDEIDTAFRRGDSGDSGVSRNIFSEFLQFTGN
                     TSNRGKVIFIAATNRPDLMDPALKRAGRFDKKIPILLPDVRERAEIFRIIINKYKFET
                     DVKDFTVFAEQTENYTGAEIETVVRKAYELANEDETEGAVLTADIISEAIKRCRPSTQ
                     QVQFMTMLAIEECDDKDLLPDKYKSLLDERNMDDR"
     gene            39286..40014
                     /locus_tag="PLG72_00190"
     CDS             39286..40014
                     /locus_tag="PLG72_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOJ79255.1"
                     /translation="MGFFKRLGALIRGFFSRLLGGVEESNPELLFEDIKNQIEKARKE
                     AEQQIIEIQTNAELIKIEMKNAEKNLEAVRARIETAQKQGDKELLIELLMHEEEYQNT
                     YEMHKATYDSAMAEVARIKEDYKIFESEMNAKLNELKTLKSQAKMAQLRENINAVNAQ
                     YTSRSNRVSNVNESMERAREMVNRKTARANAMESLSENNLDLKLKRLDMNSARERAKA
                     RAEAMLSSEQGFEVKEKAENKATQ"
     gene            40098..40904
                     /locus_tag="PLG72_00195"
     CDS             40098..40904
                     /locus_tag="PLG72_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79256.1"
                     /translation="MRARMVLSALLKLRNIGILIVAWILLAMLSSMLPLPALFGSAPE
                     LLTFAGGLAVYIVFVIQSLLSKDFQNEFNQKEKFRKIRELNRLANRLSAEAKRNTNNT
                     YLLKLKKVMEDKNDIFASYSSGEPGYLKEKIVEQTLNLVIAYLKLLNNFCIRSRELSS
                     VDVGAISARINANNRKLSFAKDTRMAEDIRKIIEMDTRLIERLKEEKKDLERISARLD
                     YMESTVNMFKHQIMSSIENEEMLEQLETAVNEAEALDAVLYERRRSRLQL"
     gene            41263..41640
                     /gene="acpS"
                     /locus_tag="PLG72_00200"
     CDS             41263..41640
                     /gene="acpS"
                     /locus_tag="PLG72_00200"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOJ79257.1"
                     /translation="MAIRCGVDIIEIDRIRKSLEELESFRSRVFTDHEICYCEGRNKA
                     KYESYAARFAAKEAVLKALGTGLSDGLVLKDIEIRNDERGKPEVVLMRRALEVYRRLG
                     AGSIDISLSHCGGYAVAFAVIET"
     gene            41669..41830
                     /locus_tag="PLG72_00205"
     CDS             41669..41830
                     /locus_tag="PLG72_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79258.1"
                     /translation="MRHTNRHEDTKDECGCMVDETKVMKSGSVYRTITGKKGRVCKSK
                     YMQVYESKG"
     gene            41837..42940
                     /locus_tag="PLG72_00210"
     CDS             41837..42940
                     /locus_tag="PLG72_00210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOJ79259.1"
                     /translation="MRVRFLQFGDLHLDAPFTSLSDMEGRPSQRRLDLKKALARLMDL
                     AIVEKVDLVLVCGDLYEHGYAGKSTIHYICGQFARLAGTPVLIIPGNHDPKLPGSYYS
                     EHRWPSNVHILESGDIFELPGANTRVYGGLDSGPADPSFINILMYHGTLDMPFDSGAF
                     QPLSGAYVEKMGFDYCALGHFHTRITGGGARGIFYNAGSPEPLGFDEPGDHGAFIAAI
                     EKEPGKKGSIRTEFVSVCARRYLDLTVDVGDCLNSEQAADAVAAQMVSAGGTADLFRV
                     TLTGYLPAGVQIDVEEIADRLRDSAFYLKITDNTQPDYDLKAISREKGLRGLFTRKML
                     ERASQAGDDEAAALVMKALYYGLEAIDEGKVCI"
     gene            42931..>44659
                     /locus_tag="PLG72_00215"
     CDS             42931..>44659
                     /locus_tag="PLG72_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ79260.1"
                     /translation="MYIGGIEIKGFGKLKNRSFSFGRGMNVIYGTNEAGKSTIQSFIT
                     AMFYGLKGGRSSGTGIPSPLKRFMPWDGLPYGGAITYTIDNGDSFRIERDFFRNTIAV
                     YDSSYNDITSSFNFGRDRLPMVGEAHLGMDESTFESTVLIRQMEVRLDNGSSSVLAAR
                     LVNMDSPDIDGLVFQRAEAVLTNAVKNKVGTERSRTQPLDKLQSELKRLETEYKALAE
                     RQREKEKLLQELSEIKAGLRRLEAQEHRLVKIGELIGTRKRIDDGMKKEARLSETVKQ
                     ISEIESTIGKLHSPDDSGGKTTQKHDETGAVQPGRYRPGMTSKAMIAFRIVLLLLCAA
                     VLLASAVLMFAPAGSEFAAVPIPPYVYAAAMPICVLIGAMLIKGILDERKNIGEKAGP
                     TADGMSAGGVAEEGADVTAGEIGEKLAALETAKRNLLSGASMMYGIKAGNVSDVRSAL
                     DDVRADLERLSRQLQNGLEEAAAMDVPLPGIFSQRELDELIYDSDINWLEEGWKTESE
                     SLRRKILDAALREKYCEGQLDEDKGETDRMQQVEEEIAAVREKIGELENTGKALRLAL
                     EVLTEAAEEI"
BASE COUNT        12641 a         9707 c        11243 g        10958 t
ORIGIN      
        1 tcggacggcg gcacatatgt gttttccagc cgttcgatct ccgcctctat cctttgcttc
       61 ttgtcaagaa atttcctgta cctctcttcg gagatcaggc ctatcttata gcctttttcc
      121 gttagcctca tatcggcgtt atcctgcctg agaagcagcc tgtattcggc tctcgaggtc
      181 attatcctgt aaggctcgga agtgcctttg gtcacaaggt cgtctatcag tacgccgata
      241 tatgcttccg atctgtccag tatcagagga ggttgctgtt tgatgaactg tgcggcattt
      301 attcccgcta taataccctg cgctgctgcc tcttcgtatc cggagcttcc attgatctgt
      361 ccggcggaaa acagtcccct gatgttcttg aattcaagac tgagcttcaa ctgcgtggga
      421 tcgatgcaat catattctat tgcataggcg cttctcatta tatggacatt ttccaaaccg
      481 gccatcgtct ttatcatttc tgtctggaca tcttccggaa ggctgctgga catgccctgc
      541 aggtacattt cctgtgtgtt cagccccatg ggttccacga aaacctggtg tctctccttg
      601 tccgcgaacc tcaccacttt atcctcaatg gaagggcagt acctgggccc tatgccctct
      661 atcagccctc cgtatagcgg cgagcggtgc agatttttct ttatgatttc atgggtatta
      721 ttgttggtgt atgtcagcca gcacagtgcc tggtcccttt cgatcgattc gtcttcgaag
      781 gaaaacggta ttatcacgtc atcgcccggc tgcggatcca ttttggaaaa gtcgatggtt
      841 ttcctgttca gccttgcagg agtaccggtc ttgaacctca taagcctgat ccccttttcc
      901 ctgaggctgt cagacagctt attagccggg aagaggccgt caggcccgcc gctgtagctg
      961 acatcgccga taaaaatctt tcctttcaga tatgtgccgg tagtgagtat gaccgccctg
     1021 ctgtaataga ccgcgccggt aagggttttt acgcctgtaa cggcggtatt gtcttcattc
     1081 gtaaggagtt ctgtgacttc cgcctgcttt atatagaggt tctcctggct ttccagggtg
     1141 tttttcatcg ccatctggta tgccttacgg tccacctgcg cccttaatga gtaaacggca
     1201 ggccccttgg ccctgttcag gaccttggac tgtatgagcg ttttgtcggc gcatttcgcc
     1261 atttcgccgc caagcgcatc cagttcgcgt accaggtgtc cttttcccgt cccgcctatg
     1321 ctcgggttgc aaggcatgtt tgctatcgcg tcaaggttga tcgagaaaac gatggttttc
     1381 atgcccagcc tggcacaggc cagcgccgcc tcacatcccg cgtgcccggc tcctacgact
     1441 gcaacatcga aatatcctgc aacgtattcc atactacctc caaaaaccgg gaacttatcc
     1501 ttatgtaccc gtaaactggt aattacatcc cggcatcatg ctgacatgta ttctttttat
     1561 catgttgaca tgcatatacc tgcattcgtt catttaccca cacaaaaccg tgaaaaaatc
     1621 tcgtttacca catcttcgct gactgattcg cctgttatcc tgccgagagc ttccgctgcg
     1681 tcccttatat cgatggtaac aaggtcgagc ggcatcccgc ctttataggc agattccgcc
     1741 gaatccaggc ccagtacggc atcctctata agctgcctgt gccgtgcgtt tgtcagcaat
     1801 acttcattgt tggccgatat ttcacctccg gcagccagtg actcgatcct gtccgccagg
     1861 tcatccattc cggtcccgtc gaccatggaa gcgactacca ccggtatgtc cccataagtc
     1921 ctgatctgct ctttcatatc ctctaccatc cggtcgtccg ccacatccgt cttgttgatc
     1981 atcactatcg tttttttgct cagcgaagtt ttcattattt ccctgtcttc atcggttatt
     2041 cctgtctggg catcgaggac tataatgacc agttcggcct ctgcagcggc agtcctggct
     2101 ttttctacac ctatcgcctc aacggggtcg tctgtgctgc ggattcccgc tgtatcaagg
     2161 aacctgacag ggataccctt tatattcaca tattcctcaa tgatatccct ggttgtcccg
     2221 gggatatcgg tcacgatcgc cctgctgctt ccgacaaggg cgttgagcag tgatgatttc
     2281 cctgcattcg gcttgcctat gatcgcagca gtcagaccat cacgcagcag tctgcccctt
     2341 gtgaacgact gtgctgtttt ccggagttct tctcttactt cttttattcc atttcgtact
     2401 gcttcgcctg tgatctcctc gatatcttcc tctggatact ctatcgccgc ctctatgcgg
     2461 gcaatgagtt ctatcatctt tctgcgggcg cccctgattt tctcagacag cctcccctgg
     2521 agctgtgctg cagctgcccg tgatccttcg tccgtttttg agtttatcag atctataact
     2581 gcttcggcct gtgccaggtc catcctgccg tttatgaaag cccgttttgt gaattcaccc
     2641 gggcatgccg cccgtgcccc gtacctcagt accagggaaa gcaccctgcg cagtacgacg
     2701 ataccgccat ggcagtttat ctctatggtg tcttcaccgg taaacgttcc tggcgcgtcc
     2761 attttcacaa gcagcacttc gtcaaccatt tgtccgtccg ccggatctac gatcctgccg
     2821 tgtacgactg aatggctcct cgtttcgctg atcttcctcc tgcctctgaa tattttttcc
     2881 gccacgctga acgcttccgg gccgctgatc cttataatgc cgatgcctcc cgtcccgtac
     2941 ggagtcgaga tggcggctat tgtttcagca tttcttcccg gcatggctct tgcgctgtct
     3001 gaacggtcac gcataccgca tcttcttcct tttgctgcta atttttatat cgcatctatc
     3061 atattcggac caggtatatt tattatagcg caaagtccga taataatatc tcaatgttat
     3121 gtcccacgat tgggaacatg ctccctggct gcagataaaa cttccagatt tttccccgga
     3181 taaattcaaa agaggacaat ccatgttgtc ctctgtcaat acaaaacaga tcgatatgaa
     3241 gtcccatccg ccaaaagaga ccaacgcgtt cacctgtccg aatgcgcctg tttcagcgtt
     3301 atgacgacct tcctgttggg gtcttcgccc acactgtatg tctcaacgta ttcattgttc
     3361 tggagcgtag aatgtattat ccttctctca taaggattca taggctcaag agagatattc
     3421 ctgcggtact tcacgacctt gtctgcaagc cttctcgcca gccttgcaag tgtttcctcc
     3481 cgcttttccc tgtagttctc tatatctatt gttaccctca tgtacttttt gctgcttttg
     3541 ttgactacga ggctcgtaag atactgcagc gcatccaggg tttcgccgcg cctccctatg
     3601 attataccgc tgtcttttcc cgatattttg aacaatacgg agtcttcgtc ttcatatttt
     3661 tctatgtcga catccacgtt catcttctcg aaaacgcttt ccagaaaaca gatcccttct
     3721 tctgccgggt ccggtttgag cctcacctgt actctcgcct gcctgctccc tattccgaaa
     3781 aggcccttgg acccttcttc cagtaccgtg acttccacgt catccatttc cgcgccaagc
     3841 tgcttcaatg cagcctctat cgcgtcatca actgtcttcc ctgcggtttc tatcactttg
     3901 ctcatctata ccagcaacct cctttatacc gccatttttc atatacatgc cgaaatcagc
     3961 gccgcttcgt ttcatttctt ctttttgccg ccttttttgc tgccgccctt tttcccgccg
     4021 gctttatttg cctttccttt gttttgtccc gacggcagtt gttttgcaga agcgtcatca
     4081 ccactatcta ttttaccatt atttccttcc tgtgattctt ctttcgaggc tgtccggctc
     4141 cccttgccgc ccgccgaagc tgcggcctct gccggtttat cctttttcag tacgaactta
     4201 ttgatataaa gctgttggaa aatcgcgaat acataaccgg tcatccagta cagcacgaca
     4261 ccggcaggaa gctggaatga cagcatcagt gtcatgacgg ggcctacata catcattgaa
     4321 tttgccgaac agccggaagc accgccctgc tggtcatttg ttttcggcat cgacagcctg
     4381 ctcgacagat atgtcgtggc cgttgcaacg atagcaagta tgaacagggg aagatatatc
     4441 ttccattcgg ggccgaaaac aacgtgcgga cggaatgaag caacctctcc caggtgcagg
     4501 ccaaggaagc tgcggaaatc tataagttcc gacttatcca ggaagttgct tacttcagac
     4561 agcatatcct gatgttcatt gaaaaagttc aaggccctta tctccctgtg gctcccccag
     4621 ctcctcagta tattttccgc tgtaaccggg agattctcaa gccccagcga tgcattgacg
     4681 gcagcctggg cctttgccgc ggcgttcact atcgcctcga tggcttcacc ggattttccg
     4741 agcatgaatt tcaaaggctg cacaattacc caatatagtg cgagtatcag gggcatctgg
     4801 atcaacaggg gaagacatcc gccggcagga ttatagttat gctcctggta gagcttcata
     4861 agctcttggt tcattgcttc cctgtcatcc ttgtaccttt tttggatgtc gttaataaga
     4921 ggctgcagtt cctgcatctt catcgttgat ttttgctgct ttacagtcag cggaagcagc
     4981 gcgagcctta ctataaatgt gaatattatt atggcaagac cgtaattttc aaaggttata
     5041 tcatagatga acttaagaaa cttgccaagc ggataagcca aaaaatcaaa cataacccgt
     5101 ttattccctt cactgttttt gaatatattc ttcttactgt aagcagagaa cctccggaat
     5161 accgtgaaat acgccaatga tgtgaatcgt cccgcgcctg gtcagcctgt ctgctgtgat
     5221 tcttttattt aacaggatca tagccgcccg gatggaaagg atggcatttc agtatgcgcc
     5281 tgacagcaag ataactccct ttgagtacgc cataccggct cagggcgtca atggcatact
     5341 gtgagcatgt aggataaaac ctgcagctcc cttttggttt caggggcgac aggtatctct
     5401 ggtaaaacct tatcattgct attaagagtt tcttgatgca tcccattttt ccttattatt
     5461 aaaagcacag gcctttttaa aaaggaattt catttccttc aggatctccg caaagcggta
     5521 ttccgtctcc acttttcttg ctgttattac tatgtcgaga ccgacaggga tatacggctc
     5581 cagatgccgg taactctccc tgatcagtct cttcaccctg tttctcttta cacttttccc
     5641 tatttttttg cttacgctta cgccaagcct gttgatctcc gtaccgttat tcagcaaata
     5701 taataccagg tacttccccg ggtaaaatct gcccttcctg tatatacgag caaattcata
     5761 atttttcttc agccgctcag tatttttcat tttaaattac ctggcaccat acctcgttca
     5821 atacgtgaca taatcttcgt gtgacatttc ccaactgcaa gcgaatgcgc catcacaggg
     5881 aaatgtcacc gtgccttttt ccgagaagcg tcacgtcaca taagacagaa aaagaccaca
     5941 ctaatgcggt cttacgctga aagaactttg cgtccttttt gtctgcgtct tctcaatact
     6001 ttcctgccgt ttttagtgct cattcttttt ctgaagccgt gctccttgct tctttgtctt
     6061 ttcttaggct gaaaagtcct taacacagtt aacacccccg ttccatgcca ctattaggta
     6121 tatagtttca aagtaagtat ttgcttttat gcgcttcttt aaacaaaaaa taagcatgta
     6181 tagtcctgaa ggacaaatac atcacgccaa ttatatatta aataccgaga taatgtcaag
     6241 aaagtattcc gatttcatcg ttttcggaac ctttccatac gttttgctat gattttccta
     6301 tataataatg aagataaaaa atggaccgct tttccgatgt tgatatctga agtgacaggc
     6361 tgtggataaa tgaagatttt ttattctatt tgtggataac tagaccattt cttaattgaa
     6421 tattgcccga aagtctgata tattattttt gctcaataat gatgatcacg tgatttccat
     6481 ttttgaacac tgtcatataa caataccttg gaggaaacat catgaacttg tcgttatccg
     6541 gcatttggcc cagagtgctc gatcttttga aaaatgaact gactgagatc agtttcaata
     6601 cgtggataaa cacaatcgaa cccatatctt ttaattcaga caccatcact cttggagtcc
     6661 ctacggattt taacaaggga atacttgaat caagatacct tacgcttata tcaaatgccg
     6721 tcagacaggt ggcattgaaa gaatatacga taaaaataac cgttccgtcc caggaacccg
     6781 cgtccaaagt aaagcagcag cctgaaacga acgtaaacga agatatttat aattcagtcc
     6841 tgaacccgaa atacactttc gatacttttg tcatcggcaa cgggaacagg cttgcacatg
     6901 cagctgctgt cgcagtcgca gaagctccgg cccaggcata caacccgctg ttcctttacg
     6961 gcggcgtcgg actcggcaaa acgcatctga tgcatgccat cggccattac atattgagcc
     7021 agtatcctca catgaaggtc ttgtatgttt cttccgagaa atttaccaat gacctgataa
     7081 acgcaataaa ggacgatacg aatgaagaat tccgaaacaa atacaggaac atggatgttc
     7141 tcctcataga cgacatccag ttcatcggcg ggaaagagag gacccaggaa gaattcttcc
     7201 atactttcaa tgcgctgtat gaagcaaata agcaaataat catatcaagt gaccgcccgc
     7261 caaaagagtt caccactttg gaggacaggc tccgctccag gtttgaatgg ggcctcactg
     7321 cggatatcca gtcaccggat tttgagacga ggatcgcgat actgaagaaa aaagctcagc
     7381 tggaaaatat cacgataccg gacgatgtac tggcgttcat agcagacaag gtagtatcca
     7441 atatccgtga actggaaggc gcgctgaaca gggtaatagc ttactcctcg ctaacagaca
     7501 ccgagatttc cgtcgacctt tgcgcagagg cgctgaaaga aatacttgcc gcttcaaaca
     7561 acaaggtgat cgatacgggc cacattatag aatcagtatc cagatacttt gatatcagga
     7621 tggaagagat cctgtcacag aagaggaaca ggaacatatc ctatcccagg cagatcgcca
     7681 tgtatctttg cagggacata atggggctat cgcttcccaa aataggagaa gaattcggcg
     7741 gaagggatca taccaccgtt atacatgcaa tccagaaaat agaggaagat atacaactga
     7801 atcctgaaat caaacgtacc gtggaagagt tgaaaagaaa tataaccgga aaataaccat
     7861 tatccccaga aattgttgat atggggataa gttgctgttg gtaatatgtt gatatcccgg
     7921 atctgatttt tacatcaact ttcagcaaca aattatcaac atcgaaatca acaggcgaaa
     7981 acctgataat ttatgggctt gtaccagtta ttcaccgaat caacagcact tactattatt
     8041 actgtttgtt atattgaaat tagataaaga atacggaggt catccagatg aaaatcattt
     8101 gctcaaaaga aaaacttctc gaagggataa acatagtaca gaaagctgtg tccgccaaaa
     8161 caccgctgcc tatacttgaa gggatcctgg tcgaagccgg tgaccaagta aagcttacct
     8221 ccaacgacct ggaaataggg attgaatgca ttatcgaagc agacgtcagg gaagaagggg
     8281 caatagtcct aaatgccagg atgttcggtg aaatcataag gaaattcccg gatgctgaag
     8341 tattgataga agtcagagag aacaacaatg ttgtaataga atgtgaaaac tcgatgtttg
     8401 aaataagagg acttgatgcg gaaggttttc ctgcgcttcc gacactggag aagaaagacg
     8461 gaataaagat aagccagaga atgctgaaag atatgataag gcaaaccata tttgcagtaa
     8521 gtactgacga aaacaggcca atgcttacag gttcgctgtt tgagtataag gatggcaagt
     8581 tcactatcgt ttcgatcgac ggttacaggc tcgcaatgag atgtttcgaa ccggagaatg
     8641 aaaatgaaac ggtaagtgcc gaaagaaagt ttgtcattcc cggaaaaacg cttaatgaga
     8701 tcgcgaagat actgcagccg gtagatgacg atatatacat atatgcagct gggaaccaga
     8761 tcctcttcga catgggcaac tgcatcatga catcaaggct tcttgaaggt gaatacctga
     8821 attatatgag cattttcccg aaagaccacg aaacaaaggt aagagtgaat acgaaagatc
     8881 tgctttcgtg ttttgaacgt gcttccctgg tgataaccag cgaagagaga agatatcccg
     8941 taagcctcga tataactgaa aaaaagatag ttatcacggc aaatactgaa gcaggcaatg
     9001 tgagagaaga acttaaaatc gatatggaag gcaaacggct tgagatatct ttctaccata
     9061 agtttttcat tgaagcattg agggccatag ataacgaaac gatagacatt tacttcacga
     9121 caagccttgg gccatgtacg ataaagccgc tgcagggaaa tgattttgct tatctgatat
     9181 tgcctattag aaggtaaact gcagtggaat aataatatgg gttaaactga acgaacggga
     9241 agaaggaatt tagttgaagg aaataaaaat atcgactgaa tatatagaac ttgagcagtt
     9301 gctgaagttt gcaggccttg tgaataaagg ttcggatgcc aaaatgctga tctcttccgg
     9361 ggcggtactg gtcaacggcc aggccgaact gaggaggggg aagaagttaa gacccggcga
     9421 tgtcgtggaa tatgacggga atgaatacat gatcacagaa taatatccgg aaggcaagga
     9481 gtttttattg aaggtaaaaa gcatctgttt gaaaaattac aggaattatg acaatgcgga
     9541 agtagaattt gcggatacat ttaacataat atacggaaat aacgcacagg gcaaaacaaa
     9601 tataatcgaa gcgattttcc tgtgtgcatc cggaagatcc caccgcacgc cgaaagacaa
     9661 tgaacttctg aaatacggga gagatttctt tgatataaag ctcgaatact caaggaccga
     9721 cagggatgaa cagatccaca tcgtatacag caatgaagac agaaaaagga taagcatcaa
     9781 tgatattcca ctgaaaaaaa taggcggttt gatgggacac cttaacgcgg tgatattctc
     9841 cccggaagat cttcttgtga taaagcaggg cccggcggaa agaaggcgct ttatcgacat
     9901 aaccctgagc cagctcagac cttcatattt tttcgatctt cagcaatatg caaaaatcct
     9961 tttgcagaga aacaatctcc tgaaagcaat ttcattgaat aatgatctgg ctgacacgct
    10021 tgaagtctgg gatcatcacc tggtcaaaac cggggcaagg ataatgaaag cgcggaagga
    10081 tttcatcaaa cggcttgata cgattgcggc cgaaaggcat gaaaggctga cgaactgtga
    10141 ggaaaaactg aaactggaat acgatccttc atttaaaatt gaggaagcag atgaggaaaa
    10201 agaagtagaa gatgcgttca ggcgaagcat aaagaagaac agggaaaaag aactgatgaa
    10261 aggaacgact ttgacgggtc cgcaaagaga tgatatagat ataatactga acggggaaag
    10321 caccaggata tatgcttcac agggacagca gagaacgtcg gtgctttcga tgaagctggc
    10381 tgagattgac ataatgaagg aagaaacggg agaatatcct gttcttctcc tggatgacgt
    10441 actgtcagag cttgatgaca ggcgaaagga atacttgctt gacagtatcg atgaaatcca
    10501 gactcttata acaagtaccg acaaaagatt ttttagcagg aacagtgacg ggtcagcatt
    10561 tttccatgta gaaagtggga tcataagaag agaatgaaaa aagggaaaga agggcaatca
    10621 tttgcgaaga aatcttaatt gagataaaat gtattgtatc tgaagaaaat caaaggaggt
    10681 tgccatgttt ttacatatcg gcggtgatgt ggtaataccc atgaaaaacg taatagcgat
    10741 cctggatata gagacttcca cgatatcgaa ggataccagg gattttctga aaactgccga
    10801 ggaagaagga ttcgtaactg cgataactga tgatcttcca agatcgttca ttataaccga
    10861 ggaagataaa aaaagcagga tttatctttc accgatttca tcggtaacgc tgcagaagag
    10921 atcaggattc atgagcgatt tcatgaacga cgagtataaa ctttgagata aagcagtttc
    10981 aaaggtttac gggaggaaga gatgagcggg ataaacactg aaaaccacaa taaatacgat
    11041 gaaaaccaga tacaggtgct tgaaggcctc gaagctgtca gaaaaagacc ggggatgtat
    11101 attggttcca cttccctgag aggacttcac catcttgtgt atgaaatcgt tgcaaacagt
    11161 atagatgaag ctcttgcagg aagatgcgac agtatagata tcgttgtaga gaaggataat
    11221 tctgttatag ttaaggacaa cgggggcggt ataccggttg gaatccatcc gaaaatggga
    11281 ataccgactg tagaagtggt acatacggtg ctccatgccg gcgggaaatt tggcggaggt
    11341 gcttacacgg tatccggagg actgcatgga gtaggcgctt ctgttgtaaa tgcgctttcg
    11401 gaatttcttg aggtccaggt ggccagagat ggaaaactat acaggcagcg ctatgaaaga
    11461 ggaaagaccg taactcccct ggaagtgata ggggaagtgg attataccgg tacgcgtact
    11521 cattttaaac cggatccgga aatattcgag gaaacggaat ttgattttga agcaatgata
    11581 acaaggtaca gggaaatggc attcctcaat aaaggtattc gcataacgct cacggacaac
    11641 aggggcgaag agccggtaaa gaaggaattc cactatgaag gcggtatagt ttcctatgtt
    11701 gaatatataa acatgggaaa ggatgtgctg cacgatgtgc cgatatattt cgatggcgaa
    11761 aaagacggtt catattgtga gatagcgatg cagtacaatg acaggtatgt agagagcata
    11821 ttcagttatg caaacaatat atgcacgact gaaggcggaa cgcacctgac gggattcaag
    11881 acagcgatca caaaagtatt taacgattat gcaagaaaat tcaattatct taaagaaaac
    11941 gacaagaacc tgatgggtga agacgtcaga gaaggactta ccgctatagt aagcgtaaag
    12001 ctgataaatc cacagtttga aggtcagacg aaaacaaaac tgggaaacag tgatataagg
    12061 aacctggtag aaaacgtagt aactgaaaaa ctgatgactt atcttgaaga aaatccgtca
    12121 gttgcaaggg cgataataga gaaatgcatt atggcgtcga gggcaaggga agctgcgagg
    12181 aaagccaggg aactcacaag gagaaagaac gctctcgaaa cgacgacgct tcccggaaag
    12241 ctggctgact gtactgaaag ggatccgtca ctttgtgaaa tctatatagt ggaaggtgat
    12301 tcagcaggcg gttcagcaaa acagggaagg gacagaaggt tccaggcgat tcttcccctg
    12361 tggggaaaga tgctgaatgt tgaaaagtca aggctggaca aggtgatcgg aaacgataag
    12421 cttatgccgg taataacggc attaggcgct gggataggtg aagattttga cctgtcgaaa
    12481 ctgaggtatg acaaagtaat tataatggcg gatgctgatg ttgacggatc acatatcagg
    12541 acgttgcttc tcacgttctt ttacaggtac atgaaaccgc tcgtggagaa agggcatgta
    12601 tatattgcat tgccgccgct ttacagggta tataaaggaa aagaggaata ctacgcttac
    12661 aacgaagttg aacttgaaag gatccagaag gagaagaact ggaagaagga agaatgtaac
    12721 gtgcaaaggt acaagggcct tggagaaatg agttcggagc agctgtggaa tacgacgatg
    12781 aacccggaaa caaggaaaat gaagagagtg actctggaag acgcagtggc ggcagataaa
    12841 atattcacca tattgatggg cgacaaggtc gaaccaagga aagacttcat acaggaaaat
    12901 gcgaagtacg tgacgaacct ggatatataa aagagttctg tatgatgaaa aaatagcata
    12961 aaataaagca atgtttcacg tgaaacattg ctttatttta tgtgaagcag tcatgtataa
    13021 actgctatga tgcaaagaat aagttcctgt tatattttca acttgtttct tttgatacgg
    13081 ttgttattat aaagacctga tactgtaccg agatataaag atgagagtaa aaagaattta
    13141 atatacagga atttattgtg ttgagtgaga aagtcaaata cagccggaga aagagaattt
    13201 tgaaaagcta tgataaagtt atattgtaat aacagagtct ctgcagcatt taatctattg
    13261 aaaatatttt gtcaaattta tcactatcag tgatattttt attgtgatat aattacatcg
    13321 accaactatt tatatcggga gtgaatgttt tggccaaggt gattgctatt gcgaaccaaa
    13381 agggaggagt cggcaaaacc actactgctg tgaatcttag cgcttgcctt gcatataaag
    13441 gcaagaaagt aatggttatc gatgtggacc cacagggcaa tactaccagc ggcctcggaa
    13501 tagacaagca aaatatcgcg agatccattt atgaagtaat aataaacgat gaaagcatag
    13561 aagaagcgct gatgccgacg atggtcgaga acctttttat ctgtccttca aacatccagt
    13621 tggtaggggc ggaagtggaa ctggtatcgg tcatatccag ggaaaccagg ctgaaaatcg
    13681 cattgcagga tgtaagggat aattatgact tcattatcat tgattgtcct ccgtcactgg
    13741 gactgctgac attgaacgca ttgaccgcgg ctgacacgat tttggtgccg atacagtgcg
    13801 aatattatgc acttgagggg ctgagccagc tgatgaacac ggtaaaactt gtacagaggc
    13861 acctgaatcc ttcacttgag gtggaaggcg tagtattgac tatgttcgat gcaaggacaa
    13921 atctctcgat acaggtagtt gaagatgtca agaagtattt caggaataaa gtatacagga
    13981 ccattatccc caggaatgta cgcttaagtg aagcccctag ttttggactg ccgataatat
    14041 tgtacgaccc aaaatccaaa ggagcggaat gctatttgga tctggcccag gaagtgatcg
    14101 actattcgga ggaggtaaag gataaatgaa aaaaggcctt ggaaaaggac tgggtgcgct
    14161 gttggggaca gaggaagccg aaaatggcgg aataatggaa ataaggataa gcgatataga
    14221 gcccaatgtc aaccagccca gaaagacttt tgatgatgaa aaactggctg cgctggccga
    14281 atcgataaag cagcacgggg tcgtacagcc gttgatcgtt cagcgggacg gggatacata
    14341 caggatagtg gcaggggaaa gaaggtggag agcggcgaga cttgccggac ttgatacggt
    14401 ccctgtaata gtgagggatc tttctgaccg gcaggtgatg gaagttgcac tcatagaaaa
    14461 ccttcagaga gaggatctga atcctatcga agaggctgag gcttacgaaa ggcttatttc
    14521 agaattcggg atgaaacagg aagaggtggc ttcggtcgta ggaaaaagca ggcctgctat
    14581 tacgaactcg atcaggttgc tttctctgaa cgatgaaata aaatccaggg tcatttccgg
    14641 tgaaatatcc agcggtcacg caagggcatt gctttcactg gacgatcaaa aactccagct
    14701 ggaagctatg caggaaatca tcgacaaaag cctgagcgtt agggaaacgg agaagctgat
    14761 aaagcagctg atgactccca aaaagcagaa agagaaaaaa gcacccgatg ccgagtatca
    14821 ggcgatcgag gaacggttca gagaaatatt cggcacaaag gtaaggataa tgaacaataa
    14881 gaagaacggg aaaatactta ttgagtatta ctcgctggaa gagttggacc ggataataaa
    14941 cctggtagaa agaatttata aaaacagtaa aaattagcca cgtcacagaa tcgatttaga
    15001 gccacgtttt gattacaggc aatatgattt ctcatttatg gtatttaaac cgttaaaacg
    15061 gaaaacagaa gctaatatgg acgatcatga aaaaaacaga aaaggtttca cgtgaaacat
    15121 tcagatgaga taaaagagtt cacagaaggg gaaggatcta tgagcatatt cgatttgcct
    15181 tatgaattga tcgtatttat cgggttgaac ttcttgctga cattgatatt gctttttatg
    15241 aatgcttcga acaggtcgaa attaaaaagg cttaaatcaa aatatgataa gttcatgaac
    15301 ggcttgagcg gcgcaagtat ggaagatgta cttgaagact gtatcgacaa acttaatgga
    15361 gtcattgaga agaacaggga attggagtac cagatcaata ctataaaaag gaacatgcat
    15421 tactgcgtac agaaagtcgg agtggtcagg tacaatgcat tcgatgatgt aggcagcgac
    15481 ctgtgctttt caattgccct ccttgacaac aatgatgatg gcgtagtgat cagcggcctt
    15541 tatgcaagag acagttcgac tacttatgca aagccgattt caagaggcaa atcaaaatat
    15601 gtattgtcgg ccgaagagat caaggccata gatctggcca ggagatcccg ggttacagat
    15661 atatatgatg actgaatttc ttctgccgat caagagatat acagaagact tttttgcgca
    15721 gtttattcag gaaagcgggt gtagttataa aaatctcgat gcgacaggag gtttatcatg
    15781 atcaaatcag gggttatttc tacagacagg acaaagtgtg taggctgcaa caaatgtata
    15841 cgggcatgca ttgtaccgca cgcaaacagg gtaactgacg aaagaaagat aaccgtcgat
    15901 tatgccaact gtattttatg cggtgaatgc cttaaggctt gtaatcatgg ggcgcgcagc
    15961 tatgttgacg acacacggga atttttcagc gagctggaaa agggaactga agtcgcagta
    16021 atagtagctc cggctttcag gttcaattat ccggacaagt ttaaaaatgt cctttcctgg
    16081 ctcaggacca aaggtgtcag gttcatctac gatgtcagct ttggcgcgga tatcaccact
    16141 tacctgtata taaaggccat agttgaaaag aaactgaata ctgtgatcgc tcagccttgt
    16201 cccgtcattg taaacagtat agagcgctat catacagaac tgataaaata cctcagtcct
    16261 ataggttcgc caatgtactg tactgccgtt tacctgaaaa aatatgacgg tttcaaagga
    16321 aaaatagcgg caatatcgcc ctgtatcgga aaaaccgatg aattcggcag ggatgatacg
    16381 atcgattaca atgttacatt tacgaaactt atggaagctt acaatgctgc ggggtatgat
    16441 cctggtgcag agacaggatt tgactctcct gaaagccttg tcggatactg gtatccgact
    16501 ccgggtggtc tgaaggagtc agtggagcag atcttcggaa ggaaatatca cataagaagg
    16561 atagaaggac cgaagctcgc ccaggaatat ctgaaagaaa tcagtcagtc acccaacgtc
    16621 atacctctgg tcatagacat attgaattgt tcggaaggct gtctgtcagg caccggtacc
    16681 gaagccggga taccgcatga tttcaaggat agggctttgt ttgagaaggc tggcgaagag
    16741 aatggcaagg tgctgtccaa caggaagcgc cagcttaaaa aactggttaa gcattttgac
    16801 aaaactctaa aaatggaaga tttcatctat acatatacaa tgcggaagga tgacgccgtc
    16861 ataaccgagc aggatattga atctgccttt gaagctctcc tgaagttcaa tgaagaggaa
    16921 agaactgtgg actgtgcagc ctgcggatac gaatcgtgcc atcagatggc tgaagcagta
    16981 ttcctcggct ataacttccc ggacaattgc attcactatt tcaaaaaaat gctggaaaaa
    17041 tcccatgatg agatttcatt ggagaagagg aaaacggagc agcttctggc caaagccaat
    17101 gacatgacag acaaacagtc tgagttcatt tcaaagctcc acagtgaagt cgaaaccgtg
    17161 gatcagataa taggtgaact ggtccaggta tatacgaata tcgtcaatga catcactaat
    17221 atcaatgaac agatgatcga tgtggagtct ctgtcacgta gcggagtaat gagtatagac
    17281 gaattgaaca gcaggttcga tcaattcttc aaaatgtctg aatctataag aaatatttca
    17341 gaacagacac atatgctatc gctgaatgca tcgatcgaag ctgcaagagc tggtgaatat
    17401 ggcaagggat tcctggtggt ggccgaagaa gtgaggaagc ttgccgatga tgcgaaaacc
    17461 gctgtggccg aaactcagga cagtaataat gttattgaag aagacattcg gaaaatcaat
    17521 gatctgctca gcaaactgca aagtgtagta gtggtggtga atgaaaatat ccagaatgtg
    17581 cttgctgcgt ctgaggaagc cagtgcatcc atgcatcaga tacaggacac catagaaaag
    17641 atcgtcagtt ccgcagaaga aatgtcctcc gctgccggat ctgaatactg accaggcggc
    17701 ctgtggataa cgaatcatga ttgatggatt atgctgataa tttaacctca gtattttgga
    17761 ccgcagactg gatcctgcct tggatgacaa gacaggatcc ggttttctga acaagccaca
    17821 aagtgacaaa agcacggtgt tgacaaagct tcgggcatat agtagaatat actacgtcgc
    17881 tttgttaaga tacggagaga tggtcgagct ggtttaaggc accggtcttg aaaaccggcg
    17941 tagtcgtgag gctaccgtga gttcgaatct cactctctcc gccaaaacga caaaaagagg
    18001 cgaaggatga gaggtttata acgcctctca taatcgcctc atagataagc ataaaagaat
    18061 tttttcttac tgcagacctg tggagaagta cccaagtagg ctgaagggga cggtttgcta
    18121 aaccgttagt aggggcaact ctagcccggg ttcgaatccc ggcttctccg ccacacaagc
    18181 cgctttcagt aatcatactg aaggcggttt ttgcattttg ataatgaatg acggtacggt
    18241 ggcttaggaa aatcttctta ttgcttctgt cttatacagc gactcagtgt ttttattgca
    18301 acttagatta ctgatcggaa aactataatg gcgtcaataa ccatgtgctg aatcaatgta
    18361 aagctccagg ccggaaaaga aactgcagtt cagttaaacg atttgatttc atattagtaa
    18421 acttatccat attaagaaaa ctttaagaac tataccctac taattgttgt ccttatatga
    18481 tataatcatt ccgccagaga ttgctgcaac cgtgtttttg gatgcatgac ccagcgggag
    18541 gttacagtcc tatggaggat aaaatagctt acggccgtaa actcaatctt atcgggatga
    18601 taatcttcat actcgcatac cttattgaca ggtttatcct taatataccc gatttacttt
    18661 atatcataat attgctgatc gtgatgtttc ttatgttcgg aggactcctg atcaggaaaa
    18721 gagcaagaga taacgagaaa taaaacatat atgaatataa aatttaaagt tcagacgttg
    18781 aagtattggt ttaagtatcg gttgaagtga aaaatctccg gagtcataca aatatatatc
    18841 agatattgtg acagataagc ccagacatca gacatttatt ggccatcagg cggctgatca
    18901 ttaacaggta gcaaggagat gaccaccatg gcggaaaaca ttaagaatgg aaaatctgat
    18961 tcccatgtac cggagcagat accgggcagg acgtgcgaag ctgcaaatga agtgcaggcg
    19021 gatgaacgac ctgtttatgc gaaagaagac aaactgttcc ttgcaggggc gctactgctg
    19081 gggatattgt tcacctggct gttttacggc aaaaacccgg gcgtttcggt cccgctgttc
    19141 gtgatagctt tctatgcact gttttttgcc tatgtacgcc ctgcacagga caaaaaggct
    19201 atgttcggat ggttcttatg tattcccgtt ttgctgattt cgatgacatt tgtcctgttc
    19261 agggatttaa cgctgatggt tctcaatata cttgcactgc cctgtctcat tatactgcag
    19321 actgtcctga ttacggggac aaacaattac aggtggtaca gcccgggcat aatagtcgat
    19381 ctgcttttcg gctttttcgc aagaggtatc gtacatgcgg caaaaccttt ctcaatgttt
    19441 gcatcgatgg tacgcagcaa aaccggtgga gagaaaaaat ccacagtgct gcggatcatt
    19501 acgggcctcg tgatcgctct tcccatactg ttgatactgt tactgattct ttcctcagcc
    19561 gacatggtat tcggaaaact tgtggaaaaa ctgcctgacc ttttgaacag tttgaattta
    19621 ggagaagtat ttattaagac cttcctgatg ctcatcattt ttatatgctc tttcagttat
    19681 atttggagcc tgaaacacaa ggaaaaattc ctcgatatcg ataacgggct cttatcagcc
    19741 aaatctgcag atgaaaaaag gcgctgggat ccggtggtcc tggttacggt aacagcttgc
    19801 atcgatcttc tttatatctt tttcgtcatc atacagtttg catacctgtt cggcagatcc
    19861 ggacttcctg aaggcttaac atattcagaa tatgcgcgca acggtttttc agaattgata
    19921 ctggtgtcac tgcttaatat gggatggctg gcgctgacac tcacgtatac gaaaaaagga
    19981 aagcgcggcc ttgatacttc gttcaggata ttgaacacga ttacggtctg ctgtacgtac
    20041 gtgatgctcc tgtcggcgta ttacaggatg ctgatgtatg aagaggcgta tggttttacg
    20101 ttcctccgtg tgatgaccca ggcgttcatg atctttctgt tcgtactttt cagtacaacg
    20161 ctggtcaggg tatggaatga ccgcatcccg ctccttaaac cctatatcgc tgccgctgtg
    20221 atcgctttta ctgtcatcaa ctatataaac gtagacgcaa tggtggcaaa gaaaaatgtc
    20281 gaccggtatt ttgaaacagg aaaaatagat atatactatt tcaattctct atcaaatgat
    20341 gcagttgcag agctcgtgag acttgtcgat gccgatgatc ccgaagttgc acaaaaagct
    20401 gccgaaatac tggcagacag gaaagaacgt atttatgaac gtactgactg gcaatcattc
    20461 aatttggcgg atcttcgtgc aaaacgggcc attagcggga aatgagtaca ccgtgtcatg
    20521 ctccggccca ggtagcttac agaaacatcc ggttccgttc agggaactta cataattacc
    20581 accgtccaat tccggcaact taactctaac ttatacaagt atatctgttc aactttgaca
    20641 gcttatagca ggataattaa ctcagccttg acagtttgtt cagtatcgtg aacaatacta
    20701 tggcgatatt tgatatgctt gagacaaata tctgaggctt gtatttgttt ttatcaaaac
    20761 cggcgctgtt tttctgagcc tttttgatta taaaaaggat ctgcatcaat atagccagcg
    20821 cagctacgca tttcagcagg atagtctcaa ggtagaatgc acagtagatt atgaaaacga
    20881 ttacgatcat gtgaacgata atgttcaggc cgttgccgaa tttgcgcacg tcgatatcga
    20941 tcagctcctc caggtcatct tccaggtcct cgtcaagttc aatctgctcg aaaccgttga
    21001 gcacaataag gttgtatata cttttgattg atgccagagt aactaatgcc gccgtcactg
    21061 cgaagaaaat gtcgtgatcc gttgacaggg caattatcgg aaacgccagt acggcaagaa
    21121 atatcagaac gctgcctttg gattgcattt tgtccccctg ataaatactg tttatgatct
    21181 ccgtgtttct gatcattata caataaaaaa catattcata tataaatagt ttagcacaaa
    21241 aagaaatcag gacaatataa aattggcata tgtcaatgat gatatatgcc aagaactgaa
    21301 attggtccct gatataaagc atttcacggt tctttcaatg cctggctgag tggccggcag
    21361 tatgactgga tgcgccggtt ttactatcag tgccgtattc ggtacagcat tattccatgc
    21421 tgactgtatc attatctgat atttccgggg tatccggaac atctccggaa ttttccggag
    21481 taaccggagc gttgccgtat gtctccggtg agtcggagga agcgccggat gttccgccgc
    21541 tgaccggttc agtgcccggt ttctcgtcag cagcaggttt gtcggcggtg gccggctggt
    21601 tatccccagt agaagtttca tcctgtgccg cagctttagc cttgctggcc atctgtgctg
    21661 caagaccggg cgtaagggtg ccgtcaggct gctttatgcc gacaaggaac tcttcatcac
    21721 atggtttgta gctgcttgtg tgaattttct tctgggatat tactttatca cccattttaa
    21781 ctgttttata ggtttccacc acatacccgt cggtgccata ctggcttttc ttcaccgttc
    21841 ccacgggcag agcaggatca tccacatatt ttgttttgta cggcgtatgg ctcagagttt
    21901 ttgtactgat aaccacggtt ttttcgggag ttgaattggt tccgaggaaa acgcagttca
    21961 ccttgttgcc ttcgacccac gaaagtattt tgataggcca gccagtgtta ttgataaagc
    22021 gcaggtcggt tccgccgtag tatgcggtgg catcctgccc gaggggaaca tatgtaacta
    22081 tgaaagagtg gttccttctt tcgaccactt caaggtctgc aagcagtaca gcattataga
    22141 gcgtgcttgt tgcctgacag atacccccgc ctatgccgtc ctccacacgg ccgtttacaa
    22201 aaacgtgagc tgacttgtaa cctgtctttt catttctcgg acccactact tcattgaaag
    22261 aaaattcttc accgggcatg agtatatagt tgtggtattt tccggcggca agcgcgatat
    22321 tgtgcatcct gtttttctca ttctgcgtcc tggtttcaaa agatgtactg taccttgcta
    22381 gttcgtccct gaacagcatc gatgccgctt tctccgatgt tatctcaggc attacatagg
    22441 tgactggcag ggtggtttcc atgttttctg ttttgttgtg ctcgtcgatt atgcgcttaa
    22501 gttcttcccg gtcgatctgc cttcctgttt tgtggggaac cagcaccaga ttgccgtttt
    22561 ccattttgta ataggcattt tccgggtcgc atactatctt gtcatacact tcgccggcat
    22621 caaaccgggt cgtttcggtt attatcacat ctggcctgat gactccgcct tttccctctt
    22681 ttatcatatt tataacaagg gatttgactt ccgctttgtc gacagcttcg ccgtgcctgc
    22741 cggactttat cacgacgctt ttgtcggaaa tgagaagtat actttccctg acctgctgga
    22801 atgccatcag ggcaaattca tcgacaaaat cgtcgatcct cgtttcatca tagttttggg
    22861 gaacgtatgt cacaatacct ttgaaactgg cccttgcgat atcatacaaa cgatcgaaaa
    22921 cattcccgct gcggccgatg gaaaatgcct tgtcggcagc ggcttcgatg tcatatacta
    22981 cgcccagatc cggatatgac ttcctaactt ctgccaggtc tgtttcaagc gttatatcaa
    23041 ggcctgaggc agggattgtg tatcttgatt ccagcagttc gattatctcg ctccttgcca
    23101 tgcccgacac atcgaggtcc cctatgtgta cgcccctgta aacgttgttg taccggagag
    23161 tgatgtatgc gaagacagac gcggatgcca gcattacgcc ggtaaaaaca agcagtatga
    23221 tcatcagttt cgatttcttt ttgcctgtat ttgcgggaag attggctgct acttcagttt
    23281 tcatatatcc aacacctttt tgataatttg gacgtttaat tatacaaaat atctgactct
    23341 tctattatcg caattgtttc ataaaatctc aacaaatttg tggcgcggga aaactgcaaa
    23401 ttttccacac aattgtagaa atgtatcgag aaaagttata gtatttatat agcagaggaa
    23461 atgattattg ttaagagtac attttcatgt atttaagggg acagaatctg atgaatgtat
    23521 ttaatgaggc tgaattcagg gatctgtgcg aaagactggg cgcagaagga acgatcaggg
    23581 acatgaccgt gagatatccg acaccaggtt acttcaacag ggtccggaac gtcatcaggc
    23641 atgacaggag aggtgaagtg gttttctgtg tcataaggcc tgacgggaaa gcaatcatga
    23701 ccaggcgaaa cgattatcct gaaaatgttt acaggatccc cacaggaggg atcggctacg
    23761 gtgaagatgt attaaacgcc gtcttcaggg aggtcaggga agaactcgga cttgaagtca
    23821 ggataaggga ttttgccgga gctgtgagga tacggtttga acatgggaat gagcacgtca
    23881 tgttctactc gtacatattc atcctggatg agatcggcgg gcggctgctt gcggatgcca
    23941 gcgacgacga gatcagcgag ataaaggaag tggacctgga tgaattcgaa caggcagtcc
    24001 gggcgctcgg aagcatcggg ggcaagtggc aggactgggg aaaattccga tacattacgt
    24061 caaatgccgt attgaatttt ttgaaacata agagtatata atcatcgaac tgtgcctata
    24121 cttatagcaa aaggtaattt ttgcaagtag gtgcagatga tgggcacgga attagttaaa
    24181 tacgaacatc agaacagtat taaaccaaaa agccgaaaaa agccggtgtt tcgcagttcc
    24241 agggcgtatg ccgcgctgac ccgggatgag cggcagataa tagatgcggc tttggaagca
    24301 agggaacagt ggaaggaagc cggcctcaac ttcaacaatg taacggatga actgctggta
    24361 gattactaca tatactggat gaaagcctgt gaatcgagat acacctatta cctcaggctc
    24421 gcaaaggaaa gagggctctc ccattatctg tgacaatgag tcatatccgg caggttgtac
    24481 aagtataaat aaaataaggg aataaaacag cggcttttgc cggtatgggg tcaggaccgt
    24541 gaattctgga gttattcttg catatgtggc aggctttata cttgtgcttg tttttgcaag
    24601 actgtttctg aaaccgctga aagtggtcat gaaactggtc ctcaatactg tgctcggcgc
    24661 gctggcgctg cttctggcca actggatcgg cggactgttc ggtttccgta ttgcgttcaa
    24721 tgtctatact tcatttatag tcggggtact cggaataccc ggatttatcc ttcttgtcat
    24781 actcaagctg ctgtttggta ccgtatagga aaataaatct annnnnnnnn nnnnnnnnnn
    24841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn natccttctt gtcatactca agctgctgtt tggtaccgta
    24961 taggaaaata aatctaaagc acttcacaaa ttccccttac aatgttaaaa taatatcatg
    25021 gccaaaggtt taatattgta tacaatatac aacattgaac tttgtgatac atgaaaaacc
    25081 ccgggtcatc ttgtgctgtg cattgtcttt catatgacct gagttgacaa atacatcagc
    25141 atcttgaaat ataaggggaa agtaaagggg agataagaat gggtaaaaac atgaaaacga
    25201 tggacggaaa taccgctgcg gcatatgttg catacgcctt caccgaagtg gcgggtattt
    25261 acccgataac tccgtcgtct gtcatggcag aacttgttga cgagtggtcg gccaatggca
    25321 gaaagaatat cttcggccag ccggtaaggg tcgttgaaat gcaatctgaa gccggagcgg
    25381 caggggttgt gcacggttcc ctgactgcag gcgctttgac tactacattt accgcatctc
    25441 agggattgct gcttatgata ccgaacctgt acaagatcgc cggcgaactt ttgccgggag
    25501 ttttccacgt aagtgccagg gcagttgcaa gccacgcgct ttccatattc ggagaccatt
    25561 ccgatgttat ggcatgccgt cagaccggag cggcgatgct ggctgaaggc agtgtccagg
    25621 aagtaatgga tctggcgcct gtggcacatc tgagcgcaat aaagggaagg gttcccttca
    25681 tcaatttctt tgatggattc aggacatccc acgagatcca gaagatcgaa gttatagaat
    25741 atgatgattt cgcaaagctt gtggactatg acgccgtgaa ggaattcagg aagagatcgc
    25801 tgaaccctga gaggccttca gtaagaggca cggcgcagaa tccggacatt ttcttccagg
    25861 cgcgcgaatc ttcaaatgtt tactacaatg aactgcctgc gatagttgaa gagtacatga
    25921 acaaaataag tgcgctcact ggcaggaagt atggactttt caattactac ggcgcaccgg
    25981 atgccgacag gatcattatt gcaatgggtt ccgtatgcga gaccattgag gaaaccattg
    26041 actatctcat ggcaaaggga gagaaggtcg gcgtcgtaaa ggtacacctt tacaggccgt
    26101 tctcggcaaa acacctgctt aaggccatcc cggagaccgc aaagaagata gctgtcctcg
    26161 acaggacaaa agagccgggt gctcttggag agcctctgta tcaggatgta tgcgccgcac
    26221 tttacgaaaa cggcaagtcg cctgtcatcg tagcaggacg ctacggactg ggttcaaagg
    26281 atacgacgcc agcccagatc gttgcagtat atgacaacct gaagtctgac aatccgaaga
    26341 accacttcac cataggcata gtcgacgatg ttacgaacct gtcgctccct gtgggagaag
    26401 aaatagacac gacggcagaa ggtactgtaa gctgcaagtt ctggggattc ggttccgacg
    26461 gtacggtagg agccaacaag aactccatca agatcatcgg cgatcatacc gacatgtatg
    26521 ctcaggcata cttcgcttat gactcaaaga agtccggcgg agtcaccatt tcacacctga
    26581 gatttggcaa aaagccgatc cgctccacgt atctcataaa caaggcggac tttgtggcat
    26641 gccacaaccc tgcatacgtc ggcaagtacg acatggtgag cgatctcaag gagaacggga
    26701 cattcctgct gaactgcggc tggtctgcag aagaacttaa cgagaaactt cccgccgata
    26761 tgaagaggat cctggcaaaa aggaacatca agctgtacac catagacgcc gtttcaatag
    26821 cgaaggaaat tggccttggc agcaggatca acatgataat gcaggctgca ttcttcaaac
    26881 ttgccaatat cattcctctc gacgatgctg tcaaatacat gaaagatgcc atagtgaaat
    26941 catatggcaa aaagggcgaa aaagtagtca atatgaacta tctcgcagta gataagggca
    27001 tcgaggcgct taagaagatc gatatcccgg ctgactgggc agatgccgtt gacgaagctg
    27061 cagcagcgaa ggatgtgccc gagttcatca gtaagatcct tgagccgatg aacaggcagg
    27121 aaggtgacaa gctgccggtc agtgcgttca aaggaagaga agacggcaca ttcccgtgcg
    27181 gtacctccag gtatgaaaag agaggcgttg ccgtggatgt tcccgaatgg cagatggaca
    27241 agtgcataca gtgcaaccag tgctcttatg tatgtccgca tgccgcaata aggcctttcc
    27301 tcctcgatga ggaagagacc gggaacgctc ccgaaggctt tgaaacgaag aaagccattg
    27361 gcaaaggatt tgaagggctc aacttcagga tgcaggtatc ggtgctcgac tgccagggct
    27421 gcggaagctg tgcgaatgta tgccctgcga aggagaaagc gctcataatg aaacctctcg
    27481 agacccagat ggaccaggtg aagaactggg attacgcaat gacggtaaaa ccgaagaacc
    27541 cgatgggacc gtccactgtc aagggcagcc agttcgaaga gccgctgctt gagttctccg
    27601 gagcatgcgc aggctgcggc gagacaccgt atgcaaaact catcacccag tnnnnnnnnn
    27661 nctttggcga cagaatgtat attgcaaatg caacaggatg ttcttctatc tggggaggca
    27721 gcgctccgtc tatgccgtac tgcaccaata aggccggttt cggaccttca tgggcgaact
    27781 ccctgtttga agacaacgca gagttcggat acggcatgta ccttgcggta aaacaaataa
    27841 ggaacaagat cgctgacaac tgcaggaaac tgcttgaaat ggaagtgccc ggagatgtca
    27901 gggcagctat cgaaggctgg cttgataata tgaatgacgg cgacggttcc aaggcagcat
    27961 ccgcacagct caaggcagcg cttgacgcat ataagggcaa tgcaggcgac gcggcaaaag
    28021 tattggtgga tgagatcctt gagaacagcg aattccttgt aaagaaatcc gtctggatac
    28081 tcggcggaga cggatgggca tatgatatcg ggtacggcgg actggaccat gtgctggctt
    28141 ccaacgagga tatcaatgtt cttgtattcg ataccgaggt atactcaaat accggcggac
    28201 agtcgtccaa ggctacccct tccggagcgg tggcccagtt cgctgcagcc ggcaagagga
    28261 ccaggaagaa agacctcggc atgatggcaa tgtcctatgg atatgtttac gttgcacagg
    28321 ttgcaatggg cgcgaaccag tcgcagctca taaaggcatt gctcgaagca gagaaatacg
    28381 acggaccttc actgataatc gcttatgcac catgcataaa ccacggtctc aggctcggca
    28441 tgggacagtc gcagcttgag gagaagaacg cagtcgaagc cggttactgg cacctttaca
    28501 ggtacaaccc gctgctgaag gaagaaggca agaatccgtt cacactggat tcaaaggagc
    28561 ctacggcatc gtacagagac ttcataatgg gcgaagtcag atactctgct ctgacaagga
    28621 cattccccga tatcgccgag cagctgttcg agcaggccga gaaggacgca aaagaaagac
    28681 tcgaggcata caagaggctt gcaaatggct gagtattgat atgtgcgggc ggtagagcgc
    28741 tcaaatgcgc agccgttctt taccaataaa tccggtaatc tggcgttgat aagagccgct
    28801 gactaagcac cggacaaatg catgattcaa agcatcggcc gggtatgcgg ctgaggtcag
    28861 tcggcagttg aaaatgctgg ttgtggccgg cgggtgatcg ttctgatgcg atcattcgcc
    28921 ggttttttta tttgctggtt ttcaggaccc tgctaatgat tgctgccttc tatatatgtg
    28981 tccggaagca tataattgat tgaactgtaa tcgattatat ccatttgatc acagcgcagc
    29041 cgggatcgga cggagggagc gtcatgcagg ggatggaaaa tgaaataaaa cagtgcttcg
    29101 ggatcgatgt caggagcata ttcccttata aagatgctta tgtcgctgtc acagcggcag
    29161 ggaaaaaact tgtcaaaaga gtgctgcttt cacccgaaag gctgaaattc gtgcactggg
    29221 ccaaagaaca ccttgtaagc aacggctttg aaggagttga caggtacctg gtcacgttgg
    29281 cgggagagcc cggtttctgg tgcaacgatt gcctgtacgt aatgactgat tatgttgaag
    29341 gacgtgaaag cagctttgat gatgacgagg acctgaaaat ggccgcagaa gctttggcgg
    29401 cactgcaccg tgcgtccgcc gggtatcttc ctcccgaagg gagcaaggtg cgggatgatc
    29461 tggggaagct cccggtatat ttttcaaagc gcctgaatga catcacgaaa atgaggaaac
    29521 aggcaagaaa agggaagagc cgttttgatc atctgttcct gcaatacgca gatcatttca
    29581 tcagcatggg agaaaacgcg atccaccgcc tttccgcatc atcctacgac aagatcgccg
    29641 acaggaccag gaaagagaga tcgttctgcc accatgacta tacacatcat aatatattgc
    29701 ttaatgacgg caaggtgacg gtcaccaatt tcgattactg ctgctttgaa ctcaaagcgt
    29761 atgacattgc taacctgatc aggcggaaaa tgcgaagatg cggctgggat atttcaaaag
    29821 cagggctcat caccgaatcc tataacagga tcctgcccct tggtatggac gagatggaaa
    29881 tagtgaggat catgctggaa ttcccgcaga agttctggag ggtcgtgaac cgctattaca
    29941 acagcaggcg cagctggtcg gaaaaagttt ttttgggaag gcttgaagag gtcattgaag
    30001 aaacaggacc ccttgacgct ttcctgaagg cttatgacag ggaattccta taaaagtcgg
    30061 atgattcaga tggtcaaaac agtcctgtta tgctgccgtc ctcacttatg tctatctgtt
    30121 ccgcagccgg gactttcggc agtccgggca tcgtcattat ttctccggta agtgccacga
    30181 tgaagccggc tccagccgac actctgatat cgcggacatt tatttcaaag ttttcaggcc
    30241 ttccgagcac tttggggttg tcggaaagcg agtattgtgt ttttgccatg cagacaggca
    30301 gatcatccag tttcatttcg gccagctttt tgatcgtttt gtcagcggcg ggcgaatagg
    30361 ttacgcccct ggctccgtaa atctcttttg ctatggtttc gatcttttcc ttcacaggag
    30421 cctttacatc atacaggagc ctgaaccggg atggaacggt atctaacagg ttaagcaact
    30481 tttccgccag ttctctgccg ccctcgccgc cttttgtgta aacctgcgaa attgctgctg
    30541 ctacgttcag gtcttcgcat ctcttcctga tatattccag ttctgccggt gtgtctgttt
    30601 caaacaggtt tattgctaca agtacaggta caccgaattt tctcaggttt tccacatgtt
    30661 tctccagatt cgggaatccg cgcttaaggg cttcaaggtc ttctccagca agcttttcct
    30721 tagggacgct gccattatac ttcagtgcgc gcacagtcgt tacgagtact gcagcgtcgg
    30781 gcacgaagcc cgcaaacctg cacttgatgt cgaagaactt ttctgcgcca agatctgctc
    30841 cgaaaccagc ttccgtaata acataatcag caagtttcag cgccagcctg gttgctttta
    30901 tgctgttgca tccatgggct atattggcga acgggccgcc atgcatcagc gcaggcgagt
    30961 gctccagggt ctggacgatg ttcggcttga tcgcgtcctt gagcaggagc gccatggcgc
    31021 cttccgcttt aagctgtgat accgttaccg gcgcccccga gtagtcatat cctacgatta
    31081 ttttaccaag ccggtttttc agatcagtga tgttttctgc aagacacaat atggccatta
    31141 tctccgatgc cgcggtaatg aggaagccgt cttcccgcgg agtgccgtct gcctttccgc
    31201 cgagacctac gactatattt ctcagggccc tgtcgttcat atccatcgcc cttttccata
    31261 ctatctgcct cgggtctatc cgaagcgcgt tcccctgatg gatatgattg tcaatgactg
    31321 cagaaagcag gttgttcgca gcggtgacgg catgtatgtc gccggtgaaa tgcaggttga
    31381 tgtcttccat cggcactaac tgcgcatatc cgccgccggc agccccgccc ttcatgccca
    31441 taacaggccc aagggacggt tcccgcaggc atatgacagc tttcttcccc atggctgtca
    31501 gggcctggcc gaggccgacc gtagtggtcg tttttccttc ccctgccggg gtcgggttca
    31561 ccgctgtgac caggatgagt ttgccgtccc tgttgttcct gactctctgc cagagactgt
    31621 ccgtcagctt agccttatat ttgccataca gttcaagttc atccgggttg atacccagag
    31681 tcgaggctac ttcagttatc ggcttcattt tgcatttctg agctatctct atatccgtga
    31741 gcatcgagtc caacctttcg cgttaagttt actgcacttt attatacatt cccgtttgct
    31801 ccaaaacaat agaaatcagt gaacgtttaa ttaacaaatc cgctgcggtt tacatattat
    31861 aaaccaggaa acccggtgag agtgccatat agacttatca ccgcgaaaca gagcatgaaa
    31921 cggcaggtga tgggatgagc gggataaaga ttggcgacat tgtcgcaaga aagtcttatg
    31981 gatgcgacgt ttttttcaag gtgacagaca taacggaacg ggacggggaa aggatactta
    32041 ctctcaaagg catcagctac aggatagagg ccgacgctcc cgaatcggac ctggaagtcc
    32101 agccggaggt aaaagtcagg gaatacaggg acaggtgctg gcgtgaagct gaaaaaaccg
    32161 cagcggacag gatatacgat atgagatact accgctatta catgccaagg agcagggggt
    32221 ggcgaaaaaa agcgtattat agagatacct caaatgacaa gcccgataaa ataaagagac
    32281 cgggaaaagt actgcatctg gatggagaca gggattacct ggagaattgc attgccgaat
    32341 ataaaaaact cgggatcgat gttgtcggcg aatatgtgcc cgaaaaggat cagcccgcaa
    32401 gggtctattc actgctttct gaacaccggc ctgatattct tgtgctgacc ggccatgacg
    32461 gcttcataaa agggaaggac agctacaacg atatttcgtc gtacaggact tcgaaatatt
    32521 tcatcgaagc ggtcaaagaa gccaggaggt tcaattccga catggacggg ctggtaattt
    32581 tcgccggcgc atgccagtcg atgtataatg aaataataaa agccggagcc aattttgcca
    32641 gcgctccttt ccgggatctt atacatgccc tcgatcctgt aagggtatgc cagaaaatcg
    32701 cgtttgcgag catcgacaca gttctggacg cagaagacgt aataaataat acgataacag
    32761 ggaagactgg tataggaggt atacagacaa ggggaaagta ccgggaagga tttccggtcg
    32821 agccgtatta aggtaagggg acacttctca gcagagaggt attcctttgt ggacgagaaa
    32881 tgtccccttt tgattgggga gatgggacac tcctcagcga aggagtattc ctttagggac
    32941 gagggatgtc cccttttgat taagcgcagg gacattcctc aaggttttcc tctgcggctg
    33001 aggaatgtca cgcgtttgat tggtgagggg acacgaaaaa ggtattgggt gctcctcatc
    33061 cataaaggaa tacaaggtac ccgcacgttt ggctgagtag tgatattgcc atatttcggg
    33121 aaatatcccc caggtatggg aatactgcgc atgacggagg gatggcacgc ttttggaaat
    33181 acatgacaaa aaccatggcg agaccggaga aaatacagga ttttgcgaaa agtacttgac
    33241 ataattgaat ttcccctgct ataatatata actttttttg acaagctatg atgtttatga
    33301 tataatttat aaaacagaag acgaggtgat cagatggctg aaaagaaaga ccttgttcaa
    33361 ataaagaaga acattgagac ctgcataggc aaaagggttc agctcaaagc caacaaaggc
    33421 aggaagaagg cttttatcaa ggaaggaata atagaaaact cttatcccag catcttcgtc
    33481 atcaagtttg aaaacgagta cgagacaaca aggagagtat ctttcagcta tacggatatt
    33541 ctgaccaaag cagtggaact tgtaatttac aaggacgaca aggagatact ggtgagctga
    33601 ccgaccaaaa cgacgattaa aagcctaaag aagcccgatc acatgatcgg gcttttttac
    33661 atccgattat tctgctacgt ccccggcacg acggtatcat gtctatttta aagtaatatt
    33721 tgagaggtcc tgtcaatata catttagaga ggaaaacgca ttcagtcatg cgggaggagg
    33781 atttcaaatg tctctggaac tggttggtga gcccattagg atcaatagaa ccattggcga
    33841 ggattccaca cagaccattg tcgagaacga tataatagtg cctgatacaa agccggacat
    33901 agcacgcata ttgctgctgg acggagatgc ctgggtcgaa ggggccgagg ctgcagcgga
    33961 cagggtgctc gtcaccggct cggtcagata caagatactc tatatttccg acgaccctga
    34021 gcagcctgta aaaagcataa cgacagtatc aacgttccag tatgcgatgg aaatacctga
    34081 taccaggcag ggcatgcaat gcagggtcaa atgcgatata gagcatcttg aatacgagat
    34141 actcaacagc aggaaagtca atgtaaaagc cataatcagt ctctcgggaa aagtcacgga
    34201 tcaggtcgag caatacataa cgagagacct gacaggttcg gacgggattc agatcctgag
    34261 gaacagggcg gctgtaaatg catatctcgg ggattgcagg acagactgca ggatcagtga
    34321 aacgctcgat atacctgtgg gcaaacctgc gatatatgaa atactgagaa atgatatcaa
    34381 gatcaccgga agggagtaca tggcaggaga cgacaagatc gccgtccggg gaaaccttaa
    34441 tatatccact ttgtatatcg cagataatga aaaccgcagc atacagttta tggagcatga
    34501 aataccgttc acgcggatga tcgacatgcc ggggatcgat gagacatgca gcaccagcct
    34561 tgatatcacg atcggagaag catccttcga ggctgaagag gacggagacg gcgaactgag
    34621 gcgcctgaac tgcgacgtga tgctggatat atatgcgcag tgctacggga ggaaagagat
    34681 agaactagtt gaagacgcat acagtccgta ttcgagagtg aacatcgaaa aggaagaact
    34741 gctgctcgat gaactggtat ccgagaatgt cacccagctg acactcaggg aaaacatcga
    34801 ggccgatgaa aacgccccgg acatttcaga acttttcaat gtacttggca aactttcact
    34861 gtcgtccggg caaataaccg acgaccgcgt catcgtagag ggcgtcgctg tctgcaatgt
    34921 actgtacctt gcctccagcg aggggcagcc tgtttactgc acggaacgcg aaataccctt
    34981 caggcagtcg atcgaggtaa aaggcgcaag agagggcatg aaccttgacg tggaaatgga
    35041 aatagagcat tacagctaca gtatagtcaa ttccagggag gtggaggtac gcctcgtaat
    35101 aggtcttaag acaaaagtcg gcaaacagct ttccgtgcct gtgatagtaa acgcacagga
    35161 gcagcccctc gatgaaaagc gggctgaggc gcggcccagc ataatcattt atttcacaca
    35221 gcccggggac accctgtgga agatagcaaa gaggtattat acgacggtcg acgagatact
    35281 gaagaacaat gacctgtacg aaaccgaaca gatcgcgccc ggaagccgga taatcatcac
    35341 aaaaaagctg tgaaccgaaa caacataata accggcgtac ctggcacggc agactgccaa
    35401 ctgacgtacc aggcgccggt aaacgactga ctgaagtgcc aggcaccctg ttgtctggca
    35461 ctttttattg tattggctat gttttttgta tataatatac attaaattgt atatgccgag
    35521 gtaatgatgt cttacattga aatacctgct tatgcgaaaa tcaacctttc tctcgatgtg
    35581 atccgcagaa gggaagatgg ttatcacgaa ctcaaaatgg taatgcagac ggtttctctc
    35641 catgataccg tatgccttga aaccacagga gacgcaggta tacaccttga atgcagcggc
    35701 ggcgctgtgc cctgtgacag cacgaacacg gcatggagag ccgcggaact tatgtttgag
    35761 cgatacggca tggcaggcgg gctgaggata tggatcacca aaaggatacc ggcggcggcc
    35821 ggcctggccg ggggcagcgc agatgcggca gcagttttga gaggcatcaa tgaactgtat
    35881 ggtctggacc tggacgcagg cgaactgaaa cggcttgggc tgcagatcgg ggcggatgtg
    35941 ccgtattgta tcgagggcgg gaccaggctc gcggaaggca tcggcgagat actcaccccg
    36001 ctgccggatt tcagcgggat cgatgtggtg gtcgtaaagc ccggcatatc ggtatcaaca
    36061 ccctgggtat acagccagct cgacctttcg aaggtgacgg acaatgacag gcctgataca
    36121 ggaaagttga tcggagcgct tgcaagcagg gacatcaaag cggcatcact gtatatgaag
    36181 aatgtcctgg aacttgttac ggtccgcagg tatgagatcg tgagatacgc aaaaagccgt
    36241 atggcggaga caggcgcgga agcgtgcctg atgagcggaa gcggaccgtc cgttttcgga
    36301 ttgttcgctg accaggtgaa agcgcagaag gcatatgcaa tgctttcaga ataccatgaa
    36361 tgggaatgct ttatcgcaaa gactgtctga gaacggttga aaaaacgcag aatgcgggta
    36421 aaggagatgt gcagctatgg caggaaagct gtcgaaggtc aatctgaacg actacaagcc
    36481 cttaagggat gtgatttttg atactctgag agaagctatc atcgtaggag agcttaaacc
    36541 cggacagagg ctcatggaag tgcagcttgc ggaaaagatg ggtgtcagca ggacgccggt
    36601 aagggaagct ataaggaaac ttgaactcga aggactggtg gagatgctcc cgaggaaagg
    36661 tgctcatgtg gcggagcttt cagtcaagga tataatggat gtgctggagg tccgtgcgac
    36721 acttgacggt cttgcgactt ccctgtcggc aacccggatc agcgaagacg aaaaaaagga
    36781 actcaggcac attctcgacc agttcatcaa ctgcgttgaa aaggaaaacc tgcaggggtc
    36841 gatcaaaaaa gacgtggaat tccacgatgt catctacaaa tcatcgagga acgacaagct
    36901 gatccagatc tccaacaacc tgagggaaca ggtccagagg ttcagggtcg tatatatcaa
    36961 ggattacagc agttcacggg aactgataag ggaacatact gaaatctacg aagccatatc
    37021 ggccggaaat ccggaagcgg ccatgagggc ggcgcagcgc catataaaga accaggagga
    37081 atcgatcata aggtcgctga agaaaaaaca cagcgaagcg gaaaaataaa gcgaggcagt
    37141 ggaggtggtc actccactac ctcgtagccg agttccgcga tctttctcct tatatcctgc
    37201 ggtttttgca gaccgggatc atatgagacc tttaccgatt gtgtcttaag gtcgatatcc
    37261 acgccgctga cgccctcgag acggccaagt tcgctgcgta tgcggccgga acagatactg
    37321 catgttatcg aagggaccgt gaatacaact tccatatgaa tcctccctgt agtattgctt
    37381 cgaacagtat tgtttgcaat ttgagcttta aaaataaatc aatatcaaac atttaataat
    37441 atttatcgta ttattgattg catcgtgaaa tcgcggccag gcagttcagg cggcagaaac
    37501 gctctgtgcg caggacatgc ccggctttgg taaaggaacg gaggggttgc ggtgccgtct
    37561 ggcgaatcct ggtatgacag ctttttaagt aaatacaggg caggtatatc ccatgaattc
    37621 ctgttctatt tcaacgtccg tgatctcgtg gacaactaca ggtacatgga caggtacatc
    37681 tacgaggaat tcatcaaaaa gaggaacttc gggatagtcg cgttctatga catctcaagg
    37741 gggctgactt tccttgacgc agggatggag aaggagttca acataataac gtccaatgaa
    37801 gcatccaatc tgatgaatgc catgccttca aagattttcc cgttcataga ccgggcgctg
    37861 aaaaatacga aaatggcact tttcatagac catatcgaga aaataatacc tgccggcgat
    37921 atagcgagca tgacgcttga ggagaggata gcgctgatat ggatatgcga atggtccgtc
    37981 agccccaaga tatcatcggt aggaagctgt atcatgatgc tggccgacaa cctggccgat
    38041 gtcagcaggg aactgctgaa atcggcctac aggattgagc ccatattcgt aggtttgcct
    38101 gatgaagacc agcggaagga atatatcgag tatttgctaa gggatggctc cgtgaagacc
    38161 gagataactg ccgacgaatt tgcaaaactc tcgtcagggc tcagcaaaaa atcgataaag
    38221 gacatcaagc tgaaatcgga agcggaagat atacccatca gtttcgaatt tatcaaggaa
    38281 aagaagcact cagtactgca gaaggaatac ggggacgtgc tggagttcat ttatcccgag
    38341 attggcttcg aggacatagg cgggatggaa aaggcaaagg aatacctgct caaaaatgtg
    38401 gtttacccga tacggaaggg agacctgagg cgtgtgccga tgggtatatt gctatgcggg
    38461 ccttccggga caggcaagtc gctgctcgtc tatgcgcttg ccaaagccag cggctttaac
    38521 tgcgtgaaga tagatatgtc caggatactt ggccagtatg tcggagaaag cgagaagaat
    38581 ttcaagaaat gcctcctcgg cgcccaatcg cagcatccgg tcatcgtatt cgtggacgag
    38641 atcgacacgg ctttcaggag gggagacagc ggagacagcg gcgtcagcag gaacatattc
    38701 agcgaattcc tgcagtttac aggcaatacg agcaacaggg gcaaggttat cttcatcgca
    38761 gccacaaaca ggcctgatct tatggatccg gcactgaaga gggcaggaag gttcgacaag
    38821 aagataccga tcctgctgcc ggacgtcagg gaaagggcgg aaatattcag gataattata
    38881 aataaatata agttcgagac agatgtgaag gactttactg tatttgcgga gcaaacggaa
    38941 aactatacgg gagcggagat cgaaaccgtt gtaaggaaag cgtatgaact ggccaatgag
    39001 gacgagacgg aaggcgccgt cctcaccgcc gatattattt ccgaggcaat aaaaaggtgc
    39061 cgtccgagta ctcagcaggt acagttcatg accatgctgg ccatagaaga gtgtgacgat
    39121 aaggatctgc ttcctgacaa atacaagagc ctgctggacg aaaggaacat ggatgacaga
    39181 taatttccat atatggctgt tttactttac attgggcatt atttgttata aattaagaat
    39241 atgatagctt agaactgcta aatatttaac ggggaggcaa atattatggg ttttttcaaa
    39301 agactaggtg ctttgataag aggatttttc agcagattgc taggcggcgt cgaagagagc
    39361 aatcctgagc ttttgtttga agacataaag aaccagatcg aaaaggcaag aaaggaagcc
    39421 gagcagcaaa tcatcgaaat ccagacaaac gctgagctga taaagatcga aatgaaaaac
    39481 gcggagaaga atctcgaagc ggtcagggca aggatcgaaa ctgctcagaa acagggagac
    39541 aaagagcttc tcatagagct gctcatgcat gaagaggagt accagaacac atatgaaatg
    39601 cacaaagcga cctacgattc ggcaatggca gaggtagcaa ggataaaaga ggactacaag
    39661 atattcgagt ccgaaatgaa tgccaagctg aacgaactca agacattgaa gtcacaggcg
    39721 aagatggccc agctcaggga gaatatcaat gccgtcaatg cccagtatac ttccaggagc
    39781 aacagggtca gcaacgtcaa cgagagcatg gaacgggcaa gggaaatggt gaacaggaag
    39841 actgcccgcg ccaatgccat ggaatcgctt agtgaaaaca acctggatct gaagctgaag
    39901 agactcgaca tgaattcggc aagggaaagg gcaaaggccc gtgcagaggc catgctttca
    39961 agcgagcagg gcttcgaagt caaggaaaag gcagagaaca aggcgaccca gtaaccgcag
    40021 acatttttac ataaaaccgg tgcagggaac atgtatatga tattcgtgta atacaagcag
    40081 gaatggtggt tggacctatg agggccagga tggttctgag cgcattgctc aaattgagga
    40141 acataggaat attgatcgtt gcgtggatac tgttggcaat gctgagcagc atgctgccgc
    40201 ttcctgcgct gttcggctcc gcgcccgaat tgctgacatt tgctggaggt ctggctgttt
    40261 atatcgtttt tgtgatacag tcccttttaa gcaaggactt ccagaatgaa ttcaaccaga
    40321 aagagaagtt caggaagatc cgcgaactga accgactggc caacaggctt tccgcagagg
    40381 caaaaaggaa cacgaacaat acctatctcc tcaagctgaa aaaagtgatg gaagacaaaa
    40441 acgacatatt tgcctcttat tcgtcaggag aacccggtta tctgaaggag aagatcgttg
    40501 aacagacatt gaaccttgtc attgcctacc tgaagctgct gaacaacttc tgcataagga
    40561 gccgtgaact gagcagcgtc gatgtcggcg ccatatccgc gaggataaat gcaaacaaca
    40621 ggaaactcag ctttgccaag gacacgcgga tggctgaaga tatcaggaag atcattgaga
    40681 tggatacaag gctgatcgaa aggctgaagg aagagaagaa ggacctggaa aggataagcg
    40741 cacggctgga ttatatggaa agcacggtaa acatgttcaa acaccagata atgtccagca
    40801 tagaaaacga ggagatgctc gagcagcttg aaactgccgt gaacgaggcg gaagcgctgg
    40861 atgcagtgct gtatgagaga cgcaggagca ggcttcagtt ataatacgaa gcaacggagc
    40921 aatgcaggca aagaaacata tgcactattc ccgggcataa acgaaacaac ggggcatatg
    40981 cgcaagagga acgaatacgc aatatgaaag tacagacaca ggggacggga aaacagatat
    41041 gtaaattccc ggcctctgtt ttgcttttct tattgaaaga actatgatat gttgtcaata
    41101 ggtacggaat gacgaatgat ttttgactat gccgtgcaaa accggacggt attagtcggg
    41161 cagttatccg taaaagcagt tccgtagcag gccgctgaac tatgtgcagc agtctgtaaa
    41221 aatgaaatct gcgaaaaccg attaaaggag ctatggatcg acatggcaat tcgttgtgga
    41281 gtcgatatca ttgagatcga caggatcagg aagtcccttg aggaactgga aagtttccgg
    41341 agcagggttt tcacggatca tgagatctgc tactgcgagg gcaggaacaa ggccaaatat
    41401 gaaagttatg ccgcaaggtt cgcggcaaag gaagccgtgc tcaaggcatt gggcaccggg
    41461 ctgtcggacg gcctggtatt gaaagatatc gagatcagga acgatgaaag aggaaaacct
    41521 gaagtggtcc tgatgcggag agcgcttgag gtctacaggc gcctgggggc gggatccatc
    41581 gatataagcc tgtcgcactg cggcgggtat gccgtagctt ttgccgtgat cgagacataa
    41641 atgaccggcg aaagtatgga actgcaaatt gaggcatacg aacaggcatg aggacacaaa
    41701 ggacgagtgc ggctgcatgg tagatgagac gaaagtaatg aagagcggga gcgtatacag
    41761 gacgatcaca gggaaaaagg gacgagtatg caaaagcaag tatatgcagg tatatgagag
    41821 taaagggtga accaggatga gggtaagatt tctgcagttc ggagacctgc atttggacgc
    41881 gccttttaca tcgctttcgg atatggaagg caggccgtcc caaaggaggc ttgacctgaa
    41941 aaaagcgctg gcgcggctga tggatctggc tatcgtagaa aaagtcgatc tggtgcttgt
    42001 ctgcggggac ctttatgagc atggttatgc cgggaaaagt acgatccatt atatctgcgg
    42061 acagttcgcc aggctggcgg gcacgcctgt gctgatcata cccgggaacc atgatccgaa
    42121 actgcccgga tcatactatt cagaacaccg gtggccttcc aatgtccata ttctggagag
    42181 cggcgatatt ttcgagcttc ccggggcaaa taccagggtt tatggcggcc tggacagcgg
    42241 acccgccgac ccgtctttta taaacatcct aatgtaccat ggcacgttgg atatgccgtt
    42301 cgactcgggc gcattccagc cgttatccgg tgcatatgtc gaaaaaatgg gttttgatta
    42361 ctgtgcgttg ggccatttcc atacaaggat aacgggaggc ggagcgcggg ggatctttta
    42421 taacgcggga agtcctgaac ctttgggttt cgacgaacct ggcgaccatg gggcgttcat
    42481 cgctgcaatt gaaaaagagc cgggtaaaaa gggatcgatc cgaacagaat tcgtctccgt
    42541 ctgcgccagg cgttatctgg acctgacggt ggacgtcggt gactgcctga acagtgaaca
    42601 ggctgcagac gcggtcgctg cccaaatggt ttcggcaggc ggaactgccg accttttcag
    42661 ggtaacgctt accggctatc tgccggccgg tgtgcagatc gatgttgaag aaatagccga
    42721 cagactgaga gattcggcat tttatctgaa gatcacagac aatacgcagc ctgattatga
    42781 cctgaaagcg atttcacgcg aaaaaggact caggggcctt ttcacacgga agatgctgga
    42841 gcgggcgtcg caggccggcg acgacgaggc cgcggcgctt gtgatgaagg cgctttatta
    42901 cgggcttgag gctatagatg agggaaaggt atgtatatag gcggtataga gatcaagggg
    42961 ttcggaaaac tgaaaaaccg ttcgttttcg ttcggcaggg gaatgaatgt gatatatggc
    43021 acgaatgaag caggaaaatc gaccatacag tcgttcatca cggcaatgtt ctatggcctg
    43081 aagggcggga ggagctccgg gacaggcatc ccttctccgc taaagaggtt catgccatgg
    43141 gacggtctgc cgtatggcgg cgcgatcaca tatactattg ataacggcga ttccttcagg
    43201 atagaacgcg attttttccg gaacaccatc gcagtgtatg actccagtta caatgatatc
    43261 acctcttctt tcaacttcgg cagggacagg ctgccgatgg tcggagaagc gcatcttggg
    43321 atggacgaat cgacatttga aagtactgtc ctcataaggc aaatggaggt caggctcgac
    43381 aacggcagtt catcggtgct ggcagccagg cttgtgaata tggacagtcc ggatatcgac
    43441 ggactggtct ttcaaagggc ggaagccgtg cttaccaatg ccgtcaaaaa caaagtggga
    43501 acagaaagat ccaggaccca gccgctggac aaactgcagt cagagctgaa gagacttgag
    43561 accgagtaca aagcgcttgc ggaaaggcag agagagaaag agaaactgct gcaggaacta
    43621 tcggaaataa aagccggact acggcggctt gaagcgcagg agcatcgtct tgtaaagata
    43681 ggagagctaa tcgggacaag gaagaggata gatgacggca tgaaaaagga agcccgtctc
    43741 agcgaaacgg tgaagcaaat aagcgagatc gaatcaacta tcgggaaact gcattctcct
    43801 gatgatagcg gcgggaaaac gacgcagaag cacgatgaaa caggtgccgt gcagccgggc
    43861 agataccggc cgggaatgac gtcgaaggcc atgattgcct tcaggatcgt actgcttcta
    43921 ttatgtgccg ctgtattgct ggcatccgca gtgctcatgt ttgcccctgc cggaagcgag
    43981 tttgccgcgg ttcccatacc gccgtatgtt tatgccgcag cgatgcctat atgcgtattg
    44041 atcggcgcga tgctgataaa gggcatactg gatgagcgaa agaacattgg tgaaaaagca
    44101 ggaccgaccg cggatggcat gtcagccgga ggcgttgcgg aagagggtgc cgatgtgaca
    44161 gccggagaga tcggcgaaaa gctggctgct ctcgaaacgg caaagcgcaa tctgctttcg
    44221 ggagcatcca tgatgtacgg aataaaagcc gggaacgttt cggacgtcag gtcagccctg
    44281 gatgatgtgc gggcagacct tgaaaggctg agccggcagc ttcagaacgg gcttgaagag
    44341 gcagccgcca tggatgtccc tttgcccggg atattttcac agcgtgaact tgatgaactg
    44401 atatatgatt cagatataaa ctggctggag gaaggatgga aaaccgaatc ggaaagcctg
    44461 cgccggaaaa tcctcgatgc tgcgctgagg gagaaatact gcgaaggaca gcttgatgaa
    44521 gataagggag aaaccgacag gatgcagcag gtggaagagg agatcgctgc agtgagggaa
    44581 aagatcggcg agctcgaaaa tacgggaaag gcactgagat tggccctgga agtgcttacc
    44641 gaagccgcgg aggagatcc
//
LOCUS       DAOSCC010000002        33432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_955_length_33432_cov_4.898463, whole genome shotgun sequence.
ACCESSION   DAOSCC010000002 DAOSCC010000000
VERSION     DAOSCC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 33432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33432
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_33432_cov_4.898463"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2226
                     /locus_tag="PLG72_00220"
     CDS             64..2226
                     /locus_tag="PLG72_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ79261.1"
                     /translation="MKRKAALLLSLVLLLTMVFPLKAFAAEMDKGLENAIRTAKAKFA
                     IPDDYKFSSSIYTSQSKTVYELSWRSQDTVDSTNINVSVDDEGNILYYYKYSQSDYKQ
                     GKRLPELSREDAREKAGQYIEHIAPGLLSELKYQEKYSDTVMDINYYLSYYRVVNGVP
                     YYNDTVNVSINRDTGELKSYSRNWTEDVEFPSREGVISPEEAQKAYRENMGLRLIYSC
                     TSEDDTLRAIPLYTTVYDNYAFVIDAYTGKKQRLVYDSYAGPQYLAADTSAQKTAIRM
                     AAEESVRLTPEEVEAIEEASGLISLDEAEKLARSTEFLGITDAHKLDGYYLNSGWPDN
                     KKYVWSLQFRKTAEDGSTTDYISVAINAKSKEITSFYSYSDTKVDGKQPIKDIAGARA
                     EADAFLKKYYPGYFEQLEYNKINEEYLNDINRKESNYTISYTRIVDGVPFPDNGVAIS
                     YDNLNGKISSFDLTWYDMEFPSVKNVIGLDDAYNILFDKIGIGLEYRYRSNVMMPYAA
                     EGKQENARAALVYALSPGKPLYIDAENGRIVYGDGSKYEEPKKVSYTDIKGHFAEKQI
                     NVLADFGIYLDGTEFRPNDVIVQKDFLMLLAKTLSYYGPVITEKSTQKEIDELYAYLM
                     REDIIRETEKAPNSAVTREEAVKYIIRALKYDKVADIKGIFSVSFKDSGSISEDLYGY
                     VAIASGLGIVKGDGVNFSPKKNTTRAEAAVMIYNYLRL"
     gene            complement(2350..3183)
                     /locus_tag="PLG72_00225"
     CDS             complement(2350..3183)
                     /locus_tag="PLG72_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOJ79262.1"
                     /translation="MDTFRIGDYVTRKSYGGDIDFRISDIAIGRNGRKVYVLRGLVYR
                     IMADSDEYDLMRRDQVEVQKELQSRLSQDGIHHIFNTRGANSNTKTSFRPKGRPGTIL
                     HIDSSPDFLEMCMNYYRDARIKAYGHVLPESEQPGSIRTLLKSTGADVLIVTGHDGLK
                     KGTFSVNSPDGYRSSRYFIRSVQEARKYEPSLDKLCIFAGACQSYFEGIMEAGANFAS
                     SPGRILIHALDPAKVGDRVALTDSRVFVTPEKIAKLTQSGLKGIGGIKTRGHYVVSSR
                     R"
     gene            complement(3344..3898)
                     /locus_tag="PLG72_00230"
     CDS             complement(3344..3898)
                     /locus_tag="PLG72_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOJ79263.1"
                     /translation="MGENGKAIKIAISGDLGSGKSTVCRYLKEKYGLDVYSTGQIQRS
                     LAQKYNMDVLSFNKYMETHPEIDEEIDTELTKIGRLDKDMVLDSRMAWHFVPDSFKVY
                     LTVDLDEAARRIMNDQRGRVETYACIEDARECLMARKKSENQRYITKYGVDCSNPDNY
                     DLIIDTTSLTPEQVAEKIMAAVRQ"
     gene            complement(4266..6068)
                     /locus_tag="PLG72_00235"
     CDS             complement(4266..6068)
                     /locus_tag="PLG72_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79264.1"
                     /translation="MVGYRNFGVAIYFTTRCLASITGIEEFEKEFSFLEKHLKISKVY
                     LETHRDDMDTPEELLRTVKRFFENKGIKVAGGITTTLFRSFKNKPLIDETGIFGCGGT
                     VTGKNAALQPRPEKGTMTTWSTMCFTDEAVRRKMKEISEFTASIFDEFILDDFFFSNC
                     TCESCIKAKGDRSWEQFRLDLMTEVSENLVIKPAKKVNPNCNVIIKYPNWYEAYQETG
                     YNTETQPMMYDGIYTGTETRDTDHSPQHLPRYGSYSLMRWMENIRPGKNGGGWIDPHQ
                     GIHNLAYYLEQAHLTLFAKGRELMLFCYTWTVDSIYIPPLGFELEKLDQIIGEMGEPV
                     GLPVYEPHHAHGEEHLYDYLGTAGIAFEPMPGFPQTDKLILLTANSAKDENVLDRMKD
                     HLFRGGDICITSGFLEAMQGKGIEAFTCARVTGGRVYAKDYLIGGFGSVFKGECYGRD
                     EVLLPVVKYMTNSADCLIAICKNDNNFPVLLRSRYANGTIYVLTTPDDYADIYKYPRE
                     VITVIRQYLMSSLGIYMEGDGKCGLFLYDNDTLIIESFMYKNTRVRLHAAGVRKLRDI
                     RNGFEAEPIQEKDGESIFEVPLQPVFYRAFKLIR"
     gene            6404..7342
                     /locus_tag="PLG72_00240"
     CDS             6404..7342
                     /locus_tag="PLG72_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ79265.1"
                     /translation="MLRVSKIALGTDHFGTTVSEEDAFRLMDIFQDAGGNCIDTARVY
                     GSWIPGGDGASEKTVGKWMKSRKNRNSIVLSTKGGHPDLDNMSKGRLSKKELESDLDE
                     SLKALGTDHIDIYWLHRDDVSRPVEDIMETLNAFISKGKIRAIGCSNWKAGRIEEANR
                     AAAVDGTDAFCASQIQWSLAATTPELLGDPTLVCMDGFEYAWYLDNGFPVFAYSSQAK
                     GFFSKAAVMGAESLSGKASARFALPENFERLDRVTEYANRSGLTPTAVALAYITCNKV
                     PAVAIVGCKKEEHLLDSLSAADVEIPAADADWLYKG"
     gene            complement(7394..9094)
                     /locus_tag="PLG72_00245"
     CDS             complement(7394..9094)
                     /locus_tag="PLG72_00245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ79266.1"
                     /translation="MLLSKLLGERFREKPADATLASHIFLLRGGYVRQVSNGIYSMLL
                     PAKRIAAKIEAIIREEMDNIGGQEVLFPVVLPYELWKESGRYDCVGSELVRFKDRTGH
                     GMLLGMTHEEAAVHLARTEARSWTKYPFMIYQIQTKFRDEPRSRGGLIRVREFTMKDA
                     YSFHTSQEDLERYYQVVLEAYHRIFRRVGLPQVVAVSSDSGMMGGKISHEFMLLTEAG
                     EDTIVVCENCDYKANSEVAVSRISHTPAEEQPLEGVYTPGIEDIDSLAAFLDTTPERL
                     MKAAVFAVQGRRKPLVVFIRGDLQVNEAKLRNIVGAEVFPLTDHEDADLCFGYIGAIG
                     LDSAKADILYDASLENETYLIGGANKPDHHIKGISIPRDVRPARFVDVAKAREGDACV
                     KCGGRLTLKRGIEVGNIFQLGTRYTESMNMTFTDSDGKQKYPIMGCYGIGVGRLLASI
                     IEASHDDYGPIWPMAVAPWQIHICMLNSSNEDVRNAGYKLYEELGRRYEVLLDDRNVT
                     AGIMFADADLLGVPVRVIVSARNVENGEAEISTRDKKMKKIVKLDEVPAVVDEIISQG
                     "
     gene            9338..9703
                     /locus_tag="PLG72_00250"
     CDS             9338..9703
                     /locus_tag="PLG72_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79267.1"
                     /translation="MAEPQRRFVERRKGPDAVVKAVWWTAGISWLLFIAALIFVETAR
                     PEIETFFDCQFDISIRNYWDDNALLYMFVILVLNFAVCTIGFIFNLARHKRKTDRFNK
                     SILILGALSLAGILWYIFR"
     gene            9928..10440
                     /locus_tag="PLG72_00255"
     CDS             9928..10440
                     /locus_tag="PLG72_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79268.1"
                     /translation="MRLKNVKIRYLLILLPLAVCLCAGLLIFARSYNGDPADAGYSAG
                     DIAGASDSAGSRAVLTDDDDGHNNGTDPSAAETGVIVNSTGFSANDAASYMSATTASS
                     NETHEAGSAEDTVFGTDLFANGSGGSEAGIDSFQPVSIGGLSYFIRINSNLAEIYDRQ
                     YKKSYEYKIG"
     gene            10506..12761
                     /locus_tag="PLG72_00260"
     CDS             10506..12761
                     /locus_tag="PLG72_00260"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOJ79269.1"
                     /translation="MTGQAGDDGAIDSTEDAPKVMPSLETDSDEFKEKYDTLKAQQTP
                     DGIGESDSPAELRPPDMSVFAAERERKYIDVEWKPVSYEAKVAGYKIKPDLSNVVNAG
                     QFEGFTDKQLKMLSRNGFLVVPSKDEQLFYVYETNEYREIPSFITVDSVLQTYHVFYD
                     FALRVLEQEYLLSDLEQLTDSMLRKTLILYDKLQDPEVKEAALKNAAYFAAAQLALEK
                     ELPSGMPEEAVSLAKAEYKLISDAAGFEASPIFGFMMDYSQYKPRGHYTRNHDFERYF
                     KALMWYGQVPFPLLKESDGKKELAVPETLQALLITYSLFAGGSGTPDVDLWENIYDPT
                     VFYVGTADDLTIYHYKDLIIKVYGTKPDPETFNDADKLAKLQEEAKKLPQPLIQAKWV
                     KELSGIDTPVGVQFRFMGQRYIPDSEILQNLCEPLVRPVPKGLDVMAVLGSDRAYDLL
                     TEVYDIDGQWEKYLEKFGEMKEKFDLLSDETWRSNLYYGWLWTLKTLTEPFGDGYPSF
                     MTNQAWQDKSLNTALASWAELRHDTVLYGKPSGADEMGGWLPEESPKGYVEPNIGAYE
                     RLLWLTRYARENLDARNILPNDLRYRMEDFEELLRFLIDCSVKELRNEELTELEYEKI
                     MFYGGTLESLTCSFADVGRWFEITSDTDKNMAVIADVHTAGNVGYLEEGVGYASHIFV
                     IVPIGGKLYLTRGAVFSYYEFTSNKRISDEEWQAMLKEGRQPSLPGWTESFTDISGPL
                     PEPDIKYVPGE"
     gene            complement(12866..14605)
                     /locus_tag="PLG72_00265"
     CDS             complement(12866..14605)
                     /locus_tag="PLG72_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79270.1"
                     /translation="MRDTLRILSRLWKYLYRYKWYGLLALVLSISSNLLALVGPLLSG
                     NAIDAIGTTPGAVDFETVFYYCALMAAFYILSSILSYILSVLMIRLTQKVIFQMRSDV
                     FNKLLDLPVRFFDSHQPGEIISRISYDIDTVNASLSNDLLQIFTSLFTVIGSLIMMIY
                     ISPVLVLVFAITVPTSILLTKYMTGKVRPLFRLRSAKLGELSGFVEEIITGQKTIKVY
                     NQEKTIIRRFDEKNREAVDSYYRSEYYGSMTGPSVNLINNLSLALVSMFGALLYLFGR
                     LTLGSLSSFVLYSRKFSGPINETANILSELQSSFAAAERVFRLIDEEPEPKDREDAVV
                     LDNVRGEVRLENVSFGYTPEKPVIRDLNIKAEEGKLVAIVGQTGAGKTTIINLLMRFY
                     DPDSGTISIDGKNIMDVTRESLRLSYSMVLQDTWLFCGTIYENIAYGKKGVSKADVIA
                     AAKAARIHNYIMQLPAGYDTVLSDDGINISQGQKQLLTIARAMLLDSKMLILDEATSN
                     VDTRTEQQIQKAMRELMRGRTCFVIAHRLSTIRNADIILVIKDGRIAEQGTHDELMAL
                     NGVYADMYNSQFN"
     gene            complement(14700..16712)
                     /locus_tag="PLG72_00270"
     CDS             complement(14700..16712)
                     /locus_tag="PLG72_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ79271.1"
                     /translation="MKTVFSYLRPYVPRMLLGLVIKFTGTIMDLLIPWILSHTIDDIV
                     PLRDVRLIFIWGGIMFLCAAFAFITNVIANRMASKVARDATEAIRHDLFVKISYMSCR
                     QVDEYTIPSLQSRLTNDTYHIQRMIGMMQRLGVRAPILLLGGIIVTLALEPVLSLTLI
                     IMLPFITLLVWLISKKGIPLFSESQRSIDKLVRTVRENIAGIRVIKALSKTAYEKKRF
                     HEVNQEVVKNETKAGVTMALTNPSMNLLLNLGLTLVIVVGAFRINAGLTKSGEIIAFL
                     TYFTIILNAMLSITRMFEIYSKGAASADRIREVLEAPEDLKLEPADHVNNGYHITFEH
                     VTFSYHRAKTPEDDADSTGDASDNAEKASVNAGNGSGSTEGNSGSTGNITGNTENTNS
                     NAGVALSAGNISGGKKGRSAADNSTPNGIKSILNDGSRNNGIQNEKQNTLEDISFALR
                     KGETLGIIGATGSGKSTIIRLLMRLYDPDEGVIRISGDDIRSIPPDELHTKFGVVFQN
                     DILFADTIGSNIAFGRDVSPEQTEKAAACAQAREFIDTLPLRYDHMLAMKSSNLSGGQ
                     KQRLLIARALAADPEILILDDSSSALDYKTDSLLRKALAENYGDTTTIIIAQRISSIM
                     HAGRILVLEDGKMLGYGTHEELLDNCEVYREIYELQMGGEAIARPG"
     gene            17011..17463
                     /gene="dtd"
                     /locus_tag="PLG72_00275"
     CDS             17011..17463
                     /gene="dtd"
                     /locus_tag="PLG72_00275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ79272.1"
                     /translation="MRAVIQRVKRAAVVIDGSPVSEISKGLLILVAVGSNDTQSDADY
                     LAEKTAELRIFEDADGKMNLSVKDVKGELLAVSQFTLYGDCRRGRRPSFTDSARPETA
                     RPLYEYFVEKLKQLAGTDVKTGVFAAHMEVELVNDGPVTILLDSGKLF"
     gene            17560..18021
                     /locus_tag="PLG72_00280"
     CDS             17560..18021
                     /locus_tag="PLG72_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ79273.1"
                     /translation="MAGIIGTNVVTQIGFIVRDIEKSKRKFAEFLGVEPPPHFDGGRF
                     EVTGTTVEGKPAPDANCLLAFFDVGPNFQIELIQPNGAKSTWQDFLDQHGEGIHHIAF
                     NVKDMDGKVRACEDFGMKCLQRGKYGDGSGEYAYMDAYNDLKCIIELLESY"
     gene            18632..19912
                     /gene="serS"
                     /locus_tag="PLG72_00285"
     CDS             18632..19912
                     /gene="serS"
                     /locus_tag="PLG72_00285"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ79274.1"
                     /translation="MIDIRLIRTEPERVKENMRKKFQHEKVGLVDEVLELDRQFRETK
                     SRCDELRSQRNSISKGIGALMAKGQKEEAEKNKARVVEINNELEELARLEEKLEKDIR
                     ERMLLIPNIIDDSVPIGRDDSENVEIERYGEPKVPDFEIPYHVDIMEKLRGIDLDSAR
                     KTSGNGFYYLCGDIARLHSAILTYARDFMIDRGFTYYIPPFMIRSEVVTGVMSFTEME
                     NMMYKIEGEDLYLIGTSEHSMIGKFIDTILDEDQLPQALTSYSPCFRKEVGAHGIEER
                     GVYRIHQFEKQEMVVVCKPEESMEWFDKLYHITVDFFRTLDIPVRVIECCSGDLADLK
                     VKSVDVEAWSPRQQKYFEVGSCSNLGDAQARRLGIRVRSREKGNYHPHTLNNTVVAPP
                     RMLIAFLENNLNADGTIRIPEPLRIYMGGKELIK"
     gene            20143..20733
                     /locus_tag="PLG72_00290"
     CDS             20143..20733
                     /locus_tag="PLG72_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79275.1"
                     /translation="MPGSMIHLLMAHKINPDSSTLFYIGNLAPDAVTAWKEKDITHFR
                     NLPDRSKALAELAARTPPDDDFAEGILLHLYTDWRWDTLARDEFIKTAGDGWFPQYRH
                     ELSLASIFAFHRTDWAADVMDRIYQCDKSEYGEIPGATADDLRDFISHVYEWYTETET
                     EASSVFTPEFVERFIDEIAPEYLRWRSERPYISTNL"
     gene            complement(20787..21815)
                     /locus_tag="PLG72_00295"
     CDS             complement(20787..21815)
                     /locus_tag="PLG72_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ79276.1"
                     /translation="MIDMRKVGTYISGLRKQKDMTQVELADMLNVSHQAVSKWERGES
                     MPDIGLLPKLSEIFGVSIDNILKGGDPGEQRKSIGTVAERIAGNDTDSVAQMINAGEA
                     DVQDVVDLAPAIKPSILDEVAEKLDGIDIEELIGLAPFLSKDVLAGLVDHVMEGSVDA
                     QHLVGIAPFLGQDDLDRLVDQIPEGKVDCHVLPGLAPFLGGKTLSKLVDKAADGTFDT
                     GILPAIAPFLNEKDFNNIIDKAASDKLDENLLISLAPFISGRSFSRLIDKIADGNISP
                     DIIVPLAPFLGEDGILKAIGKIPKDKLDTGIFTSLAPFLSGDALGRIIKNIAGREKLI
                     GQANDTHE"
     gene            22084..22458
                     /locus_tag="PLG72_00300"
     CDS             22084..22458
                     /locus_tag="PLG72_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HOJ79277.1"
                     /translation="MYNAFFGIFPILFSFTFVIVFILIALAAIRGIAEWNRNNHSPVL
                     TVDTRVVAKRTSVSHHHHGGNVDHYSTSTTYYVTFEFESGDRLELRVPYDEFGYLVEG
                     DMGRLTFQGTRFKGFERFGEPV"
     gene            23010..24470
                     /gene="rsgA"
                     /locus_tag="PLG72_00305"
     CDS             23010..24470
                     /gene="rsgA"
                     /locus_tag="PLG72_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ79278.1"
                     /translation="MNLIDYGFSPAMMPENESGIPARVISVHRERYELVCEHGTVYGV
                     LKPAVYFRSGSESFPAVGDFVLINYNPHGDSQITKTLERKSFFSRRDPSPGRGEQAVA
                     ANFDYVFVMQSLNDDFNVRRIERYVTLAWQSGGIPVVILNKADLAGDFTDRVRAAEKA
                     AGNAAVYAVSVKTGYGLDAMGEYLKPRKTTVLLGSSGVGKSSFVNALAGSDIMAVNGI
                     RESDSKGRHTTTHRQLIMLDSGAMIIDTPGMRELGMWDVSKGLGEAFDDVERWLGLCK
                     FSDCRHQTEPGCAIREAIAKHELSRERWESYLKLKREARYADNKDGYLQNKKRWEKDI
                     AKYQKQRQGSKAVQIQGDEPDHRNTGCTESFTCKVCGAFVVPEGAGSQHRNHCPRCLS
                     SLHVDNEPGDRASLCKGIMDPIGVWVRKNGEWAIIHRCRECGVLSSNRIAADDNPALL
                     MSIAVKPLASPPFPLAHLERDFYNGRHCFDKAGGIS"
     gene            24535..24897
                     /locus_tag="PLG72_00310"
     CDS             24535..24897
                     /locus_tag="PLG72_00310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79279.1"
                     /translation="MKYRILSIRDNPEYSERAIDWFASKWGIDRLEYEKSFNDCLTKN
                     ESLPQWYLAIGENDEIIGGCGLIENDFVDRTDLFPYLCALYVEEKARSHALGSKLLEY
                     ARTDAGRLGFDKLYLPDV"
     gene            complement(24989..27301)
                     /locus_tag="PLG72_00315"
     CDS             complement(24989..27301)
                     /locus_tag="PLG72_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ79280.1"
                     /translation="MTELIITSSVLILVVIILRHLLKGKISLHLQYALWALVLIRLIV
                     PITLFESPISVMNAVERSAAYKVAEQTLSKTLVYSDVIHNSEMTPDEAREAGKGTPHE
                     IRGYPSNSGYSHPQTYVFVDPLGVVLARMLRTVWLAGIAVVGLTLLLSNLSFSRKLRK
                     IRIRYQSDNCKRPIYLVKNLASPCLFGFFRPVIYITPDVAGDALKLRHVLAHEQTHYR
                     HGDHIWAAMRCFCLAVHWYNPLVWLAAALSRRDSELACDEGTIKRLGEASRLEYGRTI
                     IGLTCGKRKTMDLLCCATTMTDGIKGIRERITLIARKPKVLVPALVAAALVAAVAVGC
                     TFTGAKSENAEIIPQHSEDGADNGPVTEHVDGSSPGSNESDKQAIYGEITQIGIYYDN
                     TYYPGIEIDPVQIKDSKIISDIMDMISRSEAFSDDQLDKIRPMTKGSNKLVLTLKDGS
                     KKEFMFAYDDLYQLGYVQIDEKLLDPGYDFFRYLDDYVEYRRHETYIDSKVSDLFSKY
                     GWTADYLVNSVKVTLPSDLKHQAGDFPLKIYWAYNNELSKSVGLDFSSYLGKNATAEI
                     YKLREPLPDSLKPRRDARGVVIKHNGEIIGAYIDAGRHRGFACSLDRKSLKDVTGKDW
                     GNWIDGFIDYSDEIETMLSSMSPEELIRQYFEAMDEQDEKMLLACITRESLCSYLFAN
                     MDNNLLYNSDSAGVYSDGYSNVKSVKLIDMSELKDIENPDGVIEYRATVDFNFKKVIT
                     SESGVQQRFILLTKETEKGGWRISGVGTGP"
     gene            complement(27298..27690)
                     /locus_tag="PLG72_00320"
     CDS             complement(27298..27690)
                     /locus_tag="PLG72_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ79281.1"
                     /translation="MNVENISLTNAEWNLMECLWEASPRTGREATEYMRKHVGWSRST
                     TLTMLGRMTKKGLIRCEDNEGIKVYSPLIKREDAVIRETDDFLNRVYKGSVSLMMSAI
                     TKKQELTKEEIEELYAILREAEVKEGEK"
     gene            complement(27912..28000)
                     /locus_tag="PLG72_00325"
     tRNA            complement(27912..28000)
                     /locus_tag="PLG72_00325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27963..27965),aa:Ser,seq:cga)
     gene            complement(28034..28510)
                     /gene="tadA"
                     /locus_tag="PLG72_00330"
     CDS             complement(28034..28510)
                     /gene="tadA"
                     /locus_tag="PLG72_00330"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOJ79282.1"
                     /translation="MAHDKPACQLFTHERFMAEALMEAEKARKKAEAPVGAVVVRNGV
                     IIARGHNERESKNDSTLHAEMTAIRRACKKLGTWRLTDCDLYVTLEPCTMCAGAIIQS
                     RIRTLYIGTSDLKAGAAGSVIDVFRVDAFNHRVDVVWGVLEQECSTILKEFFKSLR"
     gene            complement(28779..28901)
                     /locus_tag="PLG72_00335"
     CDS             complement(28779..28901)
                     /locus_tag="PLG72_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79283.1"
                     /translation="MERFSKFIDMIMEFLIICILYLIFLPFRIIYNLSGKYASR"
     gene            29123..30049
                     /locus_tag="PLG72_00340"
     CDS             29123..30049
                     /locus_tag="PLG72_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79284.1"
                     /translation="MSGLIFIVFLVIVIINIFKKASESGYNGSGEHKRFERYLEDNRQ
                     NRPEIRNIEQNERTRWYGSSYKSNAGKGDAQRQNGQRRSTQEANSWQTGQSTQETAAG
                     QTRQSMADMAVKPNEPCDPNTAIMKNGAIGTKAADTNAADKTKAAYIPAATGSFLSRA
                     EDMDKVFGDTSMDVPITADQGYTVTECYGVNDTESYSASDMQYEGYPQDSFEAGNSIL
                     TDYYRDIPVAAQPVFVKDAGSEGIIGTGALVWPEVNEKDYFMSDLKAFEFTDVVVDSQ
                     IKLDASMPLFEEINKAPVKTVDKNDGTAGKAE"
     gene            complement(30303..31865)
                     /gene="hcp"
                     /locus_tag="PLG72_00345"
                     /gene_synonym="priS"
     CDS             complement(30303..31865)
                     /gene="hcp"
                     /locus_tag="PLG72_00345"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ79285.1"
                     /translation="MCSMFCFQCEQTAGGKGCTKAGVCGKSAEVANSQDELTCALIGL
                     ARAAEKTAPAGRTDELMLEGLFTTITNVNFDKDRVDDVTRRVREEKARLGGAEDFPAN
                     ELWTGAEDIVSLRSTLLLGLRGMAAYAYHAFVLGREDREVMAWFYKGMRAVGQEHSVD
                     EWLDLLMEFGHVNLRCMALLDGANTGEFGHPVPTRVSLTVEKGPFIVVSGHDLHDLKQ
                     LLEQTEGKGINIYTHGEMLPAHGYPEIKKYSHLKGNFGTAWQNQQKEFADVPGAFLFT
                     TNCLMPPKPSYADRVFTTAAVAYPGLVHIPGTDGRKDFTPVINKAVELGGWPEDKHFT
                     GINGGSWVMTGFAHNTVLGAADTVIKAVKSGAIRHFFLVGGCDGAKPGRNYYTEFVKN
                     TPKDTVILTLACGKYRFNDLDLGEIGGLPRIMDMGQCNDAYSAIQVATALAEAFNCTV
                     NELPLTLVLSWYEQKAVCILLTLLALGLKNIYIGPSLPAFFSSNVLNVLVDKFGIKPI
                     STPEEDLKAILG"
     gene            32014..32727
                     /locus_tag="PLG72_00350"
     CDS             32014..32727
                     /locus_tag="PLG72_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ79286.1"
                     /translation="METKHIEKVQSIAKDCMLFKDMNGEELSALLTCLDFKIRSFSRN
                     DIINMAGDFFEGIGVVIDGQVAVVKENIVGERIIFDILGRGELFGEMAAFTGVRKWPA
                     TIIANTECTVMHVPADKLVGQCSQTCTAHTKLIMNMLGILSRKALTLNRQLEYLSIKS
                     IRGRIASFLLEQYKKSGKTTFMLAMNRNELADFLNVARPSLSREMCRMRDEGMIDFHR
                     SSVRIRDMEALKAAAQGMH"
     gene            33008..>33432
                     /locus_tag="PLG72_00355"
     CDS             33008..>33432
                     /locus_tag="PLG72_00355"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOJ79287.1"
                     /translation="MNTKTPCESMVEMTELVLPNDTNLLGNLLGGRLMHLIDVAGAMA
                     AQRHCNRAVVTACIDSVDFRHPVKQGRAVILRAKVTWVGRTSMEVRVDVFSEDFLTGK
                     REFNSNAYLTFVAVDSEGKPVEVCGLRPETDEEKREYEAA"
BASE COUNT         8295 a         8160 c         8679 g         8298 t
ORIGIN      
        1 ggtcgaacag tggcttcggt cactgattta atacaatatt aattgtacaa gggtggtctg
       61 attatgaaaa gaaaagctgc actgctacta tcactggtct tactgctgac gatggtattt
      121 cctcttaaag cctttgccgc ggaaatggac aaagggcttg aaaatgccat caggacagca
      181 aaagcaaagt tcgccatacc ggatgactac aagttttcat cgagcatata cacctcacag
      241 tcgaaaacag tatatgaact tagctggcgc agccaggata ccgtcgactc gacaaacata
      301 aacgtatccg tggacgatga gggcaatatc ctatactatt acaagtattc gcagagcgac
      361 tacaagcaag gcaagaggct gcctgaactg agcagggagg atgccaggga aaaggcgggc
      421 cagtacatag agcatatcgc cccgggcctg ctcagcgagc tgaagtacca ggaaaagtat
      481 tctgatactg taatggatat aaattactac ctgagttatt acagggtcgt gaacggggtc
      541 ccgtattaca atgacactgt aaacgtaagc attaacaggg atacgggcga actgaagagc
      601 tattcgcgga actggaccga ggacgtggag ttcccttcgc gcgaaggcgt gatatcgcct
      661 gaagaggcac agaaggccta cagagagaac atgggactca ggctgatcta tagctgcacc
      721 tcagaggacg atacgctcag ggcgatcccg ctgtatacca ccgtttatga taactatgct
      781 ttcgtaatcg atgcctatac aggcaaaaaa cagcgcctcg tatacgattc gtatgcggga
      841 ccgcaatatc ttgctgcaga tacgagcgca caaaaaacag cgatacggat ggcggccgaa
      901 gaaagcgtca ggctgactcc tgaagaagtg gaggccatag aggaagcgtc cggactaatc
      961 agcctggatg aagctgaaaa actggcccgc agcacggaat tcctcggcat tactgacgcc
     1021 cataaattgg acggttacta tctgaactcc ggctggcctg acaacaaaaa gtatgtttgg
     1081 tcattgcagt tcagaaagac tgcggaggat ggcagtacga ccgactatat cagcgttgcc
     1141 ataaacgcga aatcgaagga aataacgagc ttctattcct acagcgatac caaggtcgac
     1201 gggaagcagc ccataaagga tattgccgga gccagggcag aagcggatgc tttccttaag
     1261 aaatattatc ccgggtattt tgaacaactg gagtataaca aaataaacga ggaatatctc
     1321 aatgacataa ataggaagga gagcaactac actatctcgt atacgcgtat tgtggacggt
     1381 gtccctttcc cggacaacgg cgttgccatc agctatgaca atctgaacgg gaagataagc
     1441 agctttgatc ttacctggta cgacatggaa ttcccgtcgg tcaaaaacgt gattggcctg
     1501 gacgatgcat acaacatcct gtttgataaa ataggcattg gacttgaata caggtaccgt
     1561 tcgaacgtta tgatgccgta tgccgccgaa ggaaagcagg aaaacgccag ggcggccctg
     1621 gtatatgcac tttcgcctgg taagccgctg tatatagatg cggaaaacgg caggatagtg
     1681 tacggggacg ggagcaaata tgaagagccg aagaaggtca gctatactga catcaaaggc
     1741 cattttgcag agaagcagat aaacgtgctt gcagatttcg ggatctacct cgacggtact
     1801 gagttcaggc ctaatgatgt gatcgtgcag aaggatttcc tgatgctgct ggcaaagaca
     1861 ttaagctact atgggccggt cataacggaa aagagcacac agaaagagat cgatgagctt
     1921 tatgcttatc tcatgaggga agacataatc agggaaacgg aaaaagcgcc gaacagcgct
     1981 gtgaccaggg aagaagcagt caaatatata ataagggctc tgaaatatga caaagtagcc
     2041 gatatcaagg gcatattcag cgtaagcttc aaggacagcg gcagcatcag cgaggacctg
     2101 tacggttacg tggcgatagc ctcaggcctt gggatcgtaa aaggcgacgg cgtcaacttc
     2161 agtccgaaga aaaacaccac cagggcggaa gctgcagtca tgatctataa ttatctccgc
     2221 ctgtaagacg cacgcactgc ctgtaaaaca cgcggtaaat accggcaggc catgggaacc
     2281 tgaaacggat gaaaaataag tatgaaaaga gggggcgttg gaccggctta ccggacagcg
     2341 ccccttactt catctcctgc tggagaccac atagtggccc ctggtcttga tcccgcctat
     2401 tccttttaag ccggactgcg taagctttgc gatcttttcg ggagtgacga agacgcgcga
     2461 atctgtcagc gccaccctgt caccgacttt tgcaggatcc agcgcatgta tcagtatcct
     2521 gcccggtgaa ctggcgaaat tcgcaccggc ttccatgata ccttcaaagt aggactggca
     2581 tgctcctgcg aaaatgcaca gtttgtcaag gctgggttca tattttcttg cttcctgtac
     2641 tgaccggatg aagtagcgcg aacttctgta cccatccggg gagtttacac tgaaagtacc
     2701 ttttttgaga ccgtcatgtc ctgtaacgat cagcacatcg gctcctgtac ttttaagcag
     2761 tgtccttatg ctgccgggct gttcgctttc cgggagcaca tggccataag cctttattct
     2821 tgcatcccgg tagtaattca tacacatctc gaggaaatcc ggactcgaat cgatatggag
     2881 gatcgtgccg ggccttcctt tcgggcggaa ggatgttttc gtattgctgt tagcgccgcg
     2941 ggtgttgaaa atgtggtgga taccgtcctg cgaaagcctg ctttggagct ctttctgcac
     3001 ttcgacctgg tccctgcgca tcaggtcata ctcgtcggaa tcagccatga tcctgtagac
     3061 caggcctctc agcacatata cttttctgcc gtttctgcct atcgctatat ctgaaatcct
     3121 gaagtctata tcgccgccgt atgattttct tgtaacgtaa tctccgatcc tgaacgtatc
     3181 catatgacca cccttcaatt tacataagtc aatactacat aatatgaagg gagggatacg
     3241 ttggtaactt ctgcggccaa aataatatac ccgggcatta aaaccggttc gtgcataaaa
     3301 acgggaggtt ctttgaacct cccgtcaagc tgcctttata ctattactga cggacagccg
     3361 ccattatttt ttccgccacc tgttccgggg tgagcgaagt cgtatctatg atcaggtcgt
     3421 agttgtccgg gttggaacag tcaacgccgt atttcgtgat atatctctgg ttttcgcttt
     3481 ttttccttgc catgaggcat tccctggcgt cctcgatgca tgcgtatgtc tcgaccctgc
     3541 ccctctggtc gttcatgatc cttcgcgcgg cttcgtcaag atcgactgta aggtatactt
     3601 tgaatgaatc cggcacgaag tgccatgcca ttcttgagtc cagcaccatg tccttgtcga
     3661 gcctgccgat tttcgtcagt tcggtgtcga tctcttcatc tatttcagga tgtgtctcca
     3721 tgtacttgtt gaaagacagc acatccatgt tgtatttttg cgcgagggag cgctggatct
     3781 gcccggtcga atagacgtcg aggccgtact tttccttaag ataccggcag actgtggact
     3841 tgccgcttcc caggtcccct gatatcgcga tctttattgc cttgccgttt tcacccataa
     3901 ctgacatctt cctttacagc aaattctgat atagcatatt atgaaaatgc tcataacaca
     3961 gcgttgtttt ataacgcatc attcattcat agaaagtttt tctgcctctg tgcgtttcaa
     4021 tgaggcgtaa catttatttt tgcctcgtta tgcgcaacac agcacatcga atgctatccg
     4081 ttaaaagaga ttataccagc aaaacttctt tttttctaca agacggccga aattttgata
     4141 tttcaccgta tgatgcttca ctgcaataaa ccggctgctg aaatgccgac tactaaaaca
     4201 cataacatgc cggcctctgc aaaacaacct ggactgacgg catcgaaaag ctgccggttt
     4261 gctttctatc ttatcagctt gaacgccctg tagaataccg gctggagcgg cacttcaaaa
     4321 atgctttcgc catctttttc ctgtatcggc tctgcctcaa agccgttgcg tatatccctg
     4381 agcttcctga cgccggctgc gtgcagcctg acccgggtat ttttgtacat gaacgattcg
     4441 ataatgagag tatcattgtc atacaggaac aacccgcatt tcccgtcgcc ttccatatag
     4501 atgccgaggc tgctcatgag gtactgtcgg atgacagtta tgacctccct gggatactta
     4561 tagatgtccg catagtcgtc aggggtagtg agaacataga ttgtcccgtt ggcgtacctg
     4621 ctcctcagca gcacagggaa gttgttgtcg tttttgcata tcgctatcag gcaatcggcc
     4681 gagttggtca tgtattttac gacgggaagc agcacctcat ccctgccata acattcgcct
     4741 ttgaatacag agccaaaacc gcctattaag taatctttcg catacaccct tccgccggtg
     4801 acacgggcgc atgtaaacgc ctcgatgcct ttcccctgca tcgcttcgag gaatccggat
     4861 gtgatacaga tatcgccgcc cctgaacagg tggtccttca tcctgtccag gacattttca
     4921 tcctttgcgc tgttggcagt gagcagtatg agtttgtcgg tctgcgggaa gcctggcatt
     4981 ggttcgaatg cgatccctgc tgtgccaaga tagtcataca aatgctcctc gccgtgggcg
     5041 tggtgaggct cgtacacggg cagtccgacg ggttcgccca tctcgccgat gatctggtcg
     5101 agtttttcaa gttcgaaacc aagaggcggg atataaatac tgtcgactgt ccacgtgtag
     5161 cagaacagca tcagttcacg gcctttcgca aacagggtca gatgcgcctg ttccaggtaa
     5221 taggcgagat tgtggatgcc ctggtgcgga tctatccagc ctccgccgtt cttgccgggc
     5281 ctgatgtttt ccatccacct catcagggag taactgccat agcggggcag atgctgcggc
     5341 gaatggtccg tatcccttgt ctcggtgccg gtatatatcc cgtcgtacat catcggctgc
     5401 gtttccgtgt tgtagcctgt ctcctggtat gcttcatacc agttggggta cttaataata
     5461 acattgcagt tcgggttgac ttttttcgcg ggctttatga cgaggttctc ggagacttcc
     5521 gtcatcaggt cgagcctgaa ttgctcccaa ctcctgtcgc cttttgcttt tatgcaggat
     5581 tcgcaggtgc agtttgagaa gaagaaatca tcaagtatga attcatcaaa aatcgatgcg
     5641 gtgaattccg agatttcctt catcttcctc ctcacggctt cgtccgtgaa gcacatcgtt
     5701 gaccaggtag tcatggtccc tttttccggc ctgggctgta aagctgcgtt ttttcctgtt
     5761 actgtcccgc cgcacccgaa tattcctgtt tcatcgatga gaggcttgtt tttgaagctt
     5821 ctgaaaagcg tcgttgtgat gcctcccgcc acttttatgc ctttattctc gaaaaaccgt
     5881 tttacggtcc tgagcagttc ctccggggta tccatgtcat ccctgtgggt ttccaggtaa
     5941 acttttgaga ttttcaggtg tttctccaga aagctgaact ccttctcaaa ttcttcaatg
     6001 cccgtgatgc ttgcaaggca cctggttgtg aaatagatcg ctactccgaa atttctgtaa
     6061 ccgaccatat gcatgacctc caaaataaca taatcgattg cctgaacggc tttattgcgc
     6121 cggtattgat gcttgccggc caatagcccc ggacggtgat caggtgtgat ttggccgccg
     6181 ctaataaaaa attaacagtt gtcaataata ttataaccca gctttcgcct gttggattgt
     6241 cagcaattgc cctggtgttc tcaaaaattg cagtaattcg ataggaattg ttattgccgt
     6301 tggctttgtg gtaagtatga cgtaaaagtc tctttttgaa agggtgaatg gattgaggta
     6361 ccttgatatt aaaatacccg gatccgtcga tacatgcaag gatatgctgc gggtctcgaa
     6421 aatcgctctc ggtacggacc atttcgggac aactgtaagt gaggaggatg ctttccgact
     6481 gatggacata tttcaagacg cgggaggcaa ctgcattgat acagcaaggg tctatggatc
     6541 ctggataccc ggcggggatg gagccagcga gaagactgtc gggaaatgga tgaaatcgag
     6601 gaagaacagg aacagcatcg tactgtctac aaaaggcggc catccggacc tcgacaatat
     6661 gagcaaaggg aggctttcaa aaaaggaact tgagagcgat ctcgacgaga gccttaaggc
     6721 cctcggaacc gatcatatcg atatatactg gctgcacagg gacgatgtat cgcgcccggt
     6781 tgaagacatt atggaaacgc ttaatgcttt catatccaaa gggaaaatac gtgccatagg
     6841 ctgttcaaac tggaaggcgg gaaggatcga agaagcgaac cgcgccgctg ccgtggacgg
     6901 gaccgatgcg ttctgcgcca gccagatcca gtggagcctt gcagcgacaa cgcctgaact
     6961 cctgggagat cctacacttg tctgcatgga cggtttcgaa tacgcatggt acctggataa
     7021 tggtttccct gtgtttgctt actcgtcgca ggcaaaggga tttttctcca aggcggccgt
     7081 gatgggtgcg gagagcctga gcggcaaagc atcggcaaga ttcgccctgc cggagaattt
     7141 tgaaaggctg gatcgtgtga ctgaatatgc aaatcggagc ggactgacgc cgacagccgt
     7201 tgccctggcg tatatcacct gcaacaaggt gcctgcggtc gccatcgtgg ggtgcaaaaa
     7261 ggaagagcac ctgctcgatt cattgtcggc agcggatgtt gaaatccctg ctgctgatgc
     7321 cgactggcta tataaagggt gaaaaacagg gttattggtg gaacggggag ggcaaacccc
     7381 ggtcagccag gattcagccc tgtgaaatga tttcatcgac aacagcgggg acttcgtcaa
     7441 gcttcacgat tttcttcatt tttttatccc ttgtcgagat ctccgcttcg ccgttttcaa
     7501 cattgcgtgc gcttacgata accctgacag gcacgccaag caggtctgcg tcggcaaaca
     7561 tgatgcctgc cgtaacgttg cggtcatcga gcagcacttc atacctgcgc ccaagttctt
     7621 catacaattt gtaaccggca ttgcgtacgt cctcattgga gctgttcagc atgcatatgt
     7681 gtatctgcca gggagccaca gccatcggcc agattgggcc ataatcgtca tggctggctt
     7741 ctatgatgct tgcgagcagc cttccgacac ctatgccgta acagcccata attggatact
     7801 tttgtttccc gtcggaatct gtaaaggtca tgttcatgct ttcggtatac ctggtaccta
     7861 gctggaagat attgcccact tcgatgcccc gtttcagcgt cagtctgccg ccgcatttca
     7921 cacaggcatc accttccctg gctttggcaa catcgacgaa cctggcgggc ctgacatccc
     7981 ttggtatgct gatccccttt atatgatggt ccggcttgtt ggctcctccg ataagatatg
     8041 tctcgttttc aagcgaagca tcgtacagaa tatcggcttt tgcactgtca agaccaatgg
     8101 cgccgatata accgaaacag aggtctgcgt cttcgtgatc cgtgagcggg aatacttccg
     8161 cgcctactat gttcctcagc ttggcctcgt tgacctgcag atcgccgcgt atgaatacga
     8221 ccagcggttt tcttctgcct tgcactgcaa acactgcagc cttcatcagc ctttccggag
     8281 tcgtgtccag gaatgcggcc agcgaatcga tatcttctat gccgggcgta tatactccct
     8341 cgagaggctg ttcctccgcc ggcgtatgac tgatacggga tacggccact tctgaattgg
     8401 ctttgtaatc gcaattttca caaacgacta tggtatcttc ccctgcttca gtaaggagca
     8461 tgaactcgtg ggagatcttc cctcccatca tgccgctgtc cgagcttacc gccaccacct
     8521 ggggaaggcc gacacgcctg aagatcctgt ggtaagcttc caggaccacc tggtagtagc
     8581 gttccaggtc ttcctgcgag gtgtggaacg agtacgcgtc tttcatcgtg aactcgcgta
     8641 cccttatcag gccgcccctg gatctcggct cgtccctgaa tttcgtctgt atctggtata
     8701 tcatgaaggg atatttcgtc caggatcttg cctcggtcct tgcaaggtgg accgccgcct
     8761 cttcatgggt catgcccagc agcatgccgt ggcctgtcct gtccttgaac ctgacaagct
     8821 cgctcccgac gcagtcatag cggcctgact ccttccagag ttcatagggg agcacgaccg
     8881 ggaacaggac ttcctgcccg cctatattgt ccatttcctc gcggatgatc gcttcaattt
     8941 tagcggctat cctcttcgca ggcaaaagca tcgagtagat gccgttggac acctgcctta
     9001 catacccgcc gcgcagcagg aaaatatggc tcgccagcgt ggcatcagcc ggtttctccc
     9061 tgaacctttc gccgagaagt ttgcttaaca gcatgcttat cagatccttc ccccgtgata
     9121 cttaaatttg tataagttta gcaaacagcg ggcattattg tcaaggaatg caggtttcag
     9181 gtatggtatg attatgcggg tctgccgaaa atgactttta caaaagccgc ccggatgtga
     9241 tcagcccctc gaaaaaagct gtcgcaggag cgaaaatgat tccggatttt cttgtgatta
     9301 ccataattat cttgatataa tataataggg tggtcatatg gcagaacctc agagaagatt
     9361 cgtggagagg cgcaaaggtc cggatgcggt agtcaaagcc gtatggtgga cggccggaat
     9421 aagctggctg ctcttcatag ctgcgcttat ttttgtagag acagcccggc ctgagatcga
     9481 aacattcttt gattgtcagt tcgacatatc gataaggaac tattgggacg ataatgccct
     9541 tctttacatg ttcgtcatcc tggtgctgaa ttttgccgtc tgcaccatcg gtttcatatt
     9601 caacctggca aggcacaaaa ggaagacgga ccgtttcaac aagtccatat tgatccttgg
     9661 ggccctgtca ttggccggca tattatggta cattttccgt tgagagccgt ctgcatttga
     9721 cggtggttcc gcgtcggagc gtgtttctgg aagagcatat aatcagaata tattcatccg
     9781 ggagtgtacg atagagatac atatatgagt gtacgctcta tttgcatatc gggtttacaa
     9841 atcgtgaaac ttatcgccaa caaaatatgt ctaaaagaca aaatatgtat gcatcgcaaa
     9901 aatgcaaacg tgcaaaagga aatgcatatg cgtctcaaga atgtaaaaat acgatacctt
     9961 ctgatattat tgccgctggc ggtatgcctt tgtgccggcc tgctcatttt tgcacgaagc
    10021 tataatggag acccggctga cgcgggttat tccgccgggg atattgccgg cgcaagtgac
    10081 tccgccggca gcagggccgt tcttaccgat gatgacgacg gccataacaa cggcacagat
    10141 ccttctgcag ctgaaacagg cgttattgtg aacagcacgg gcttttctgc caatgatgcg
    10201 gcttcttata tgagcgccac aacagcttcg tctaatgaaa cgcatgaagc aggcagtgca
    10261 gaagatactg tttttggtac agatttattt gccaacggct caggcggatc tgaggccggt
    10321 atcgattcct tccagccagt cagtataggc ggcttgtctt attttattcg cataaacagc
    10381 aacctggcag aaatatatga ccgacagtac aaaaaatcgt atgagtataa aatcggttag
    10441 ctgcattgtt cacatcatgt gacggcacag gaggcgatgc ctccggcaag acggctggag
    10501 gggatttgac cggccaggca ggtgatgatg gcgcaattga cagcacggaa gatgccccca
    10561 aagtgatgcc gtcccttgaa actgattcgg atgaatttaa ggagaaatac gataccttaa
    10621 aggcacagca gacaccggac ggcatcggag aaagcgactc accggcagag ctccggcccc
    10681 cggacatgtc cgtttttgcg gcagagaggg aaaggaagta catagatgtg gagtggaagc
    10741 ctgtcagcta tgaggccaag gttgccgggt acaaaataaa acccgacctg tctaatgtgg
    10801 taaatgccgg ccagtttgaa ggctttacgg ataagcaact gaaaatgctg tccaggaacg
    10861 ggttcctggt tgtcccgtca aaggacgagc agctgtttta cgtatatgaa acaaatgaat
    10921 acagggagat cccaagcttt atcacggtcg actccgtact gcagacatat cacgtgtttt
    10981 atgattttgc actcagggtc ctggaacagg aatatttgct tagtgacctt gagcagctta
    11041 ctgacagcat gctgaggaaa acgttgatcc tttatgataa gctgcaggac ccggaagtaa
    11101 aggaagctgc gctgaaaaac gctgcctatt tcgcggctgc gcaactggca ctcgaaaagg
    11161 aactcccttc aggtatgcct gaggaagctg tgagccttgc gaaagcggaa tacaaactga
    11221 tttctgatgc agcgggcttt gaggcgtctc ccattttcgg gttcatgatg gactacagcc
    11281 aatataagcc gagggggcac tacaccagga accatgactt tgaaaggtat ttcaaggcct
    11341 tgatgtggta cggccaggtc ccgttcccac tgctcaaaga atctgacggg aaaaaggaac
    11401 tcgctgtccc ggagacactg caggctttgc tcataacata cagcctgttt gcgggcggga
    11461 gcggcacacc ggatgtcgac ctgtgggaaa acatttatga cccgacggta ttttacgtgg
    11521 gcaccgcaga cgacctgacc atataccatt ataaagacct gataataaag gtctatggca
    11581 caaaaccgga ccctgagact ttcaatgacg cagataaact tgcaaaactg caggaggagg
    11641 cgaaaaagct gcctcagccg ttgatacagg caaaatgggt caaggaatta tccggcattg
    11701 acactcctgt cggcgtacag ttcaggttca tgggacagag gtatataccc gattcggaga
    11761 tactccagaa cctgtgcgag cctttagtga gacctgtgcc gaagggactg gacgtcatgg
    11821 cagtcctggg gtcggaccgg gcttacgatc tgcttacgga agtatatgat attgacgggc
    11881 aatgggaaaa atacctggag aaattcggtg aaatgaaaga gaagtttgat ttgctgtctg
    11941 acgaaacctg gcgatccaac ctgtattacg ggtggctgtg gacgctcaaa acactgacag
    12001 agccttttgg cgacgggtat ccgtcgttca tgaccaacca ggcgtggcag gacaaatcgc
    12061 ttaatacggc gctggcaagc tgggctgaac tcaggcatga caccgtactt tacgggaaac
    12121 cgtcgggggc cgacgaaatg ggagggtggc ttcccgagga atcgcccaaa ggctatgtgg
    12181 aaccgaacat aggcgcctat gaaaggctcc tgtggctcac caggtatgca agggaaaacc
    12241 tggatgcaag gaacatactg ccaaatgacc tgagatacag gatggaggat ttcgaggaac
    12301 tgctccgatt cctgatcgac tgctccgtca aggaactcag gaacgaagaa ctgaccgagt
    12361 tggaatatga aaagataatg ttctacggcg gcacgctgga gagccttacg tgttcatttg
    12421 ccgatgtcgg cagatggttc gagatcacgt cggataccga caaaaacatg gctgtcatag
    12481 ctgatgtcca caccgcagga aacgtaggtt acctggaaga aggagtggga tacgcgtccc
    12541 atatattcgt aatcgtgccc ataggcggga agctatatct gacaagaggg gccgttttca
    12601 gctattacga gttcacgagc aacaaaagga tctcggatga agaatggcag gcgatgctga
    12661 aggagggcag acagccttcg ctgcctggct ggacggaatc gttcactgat ataagcgggc
    12721 ctctgcctga gcctgatata aaatatgtac caggtgaatg ataccgcgtt tgaaatctga
    12781 tgctgtactt taagtggtat gatgccattt tcaggacggg ttcgatctat acagacgggc
    12841 ccggttttta tagcctgcgg accagctaat tgaactgcga attatacatg tccgcatata
    12901 ccccgttgag tgccataagc tcgtcatgcg tgccctgttc tgcgatcctg ccgtccttta
    12961 tgaccaatat gatgtcggcg ttgcggatcg tggacaggcg gtgggctata acgaagcagg
    13021 tcctgccccg catcagttcg cgcatcgcct tctgtatctg ctgttccgtc ctggtgtcca
    13081 catttgatgt ggcctcgtcc aggatcagca ttttcgaatc cagcagcatt gcccttgcga
    13141 tggtgagaag ctgcttctgc ccctgggata tgtttatgcc gtcgtcactg agcacggtat
    13201 catagcctgc cggcaactgc attatataat tgtgtatcct cgcggctttt gctgccgcta
    13261 tgacgtcggc cttcgacaca ccttttttcc cgtaggcaat gttttcatag atcgtgccgc
    13321 agaacagcca tgtgtcctgc agtaccattg aatatgagag ccgcaggctt tccctcgtca
    13381 cgtccatgat gtttttcccg tcaatggaga tcgtcccgct gtccgggtcg taaaacctca
    13441 tcagaaggtt tatgatcgtc gtttttccgg ctccagtctg gccgacgatg gctacgagct
    13501 ttccttcctc cgcctttata ttgaggtccc ggatgaccgg tttctcgggc gtatagccga
    13561 aggatacatt ttccagacgg acttctccgc gcacgttatc aaggaccacg gcgtcctccc
    13621 tgtcctttgg ctccggctct tcgtcgatca gcctgaaaac acgctcagcc gccgcaaagg
    13681 atgactgcag ctcgctgagg atattcgccg tttcgtttat cggccctgaa aatttccgcg
    13741 aatacaatac gaaggacgac aggctgccca gcgtcaggcg gccgaacagg tacagcagcg
    13801 cgccgaacat actgaccagg gccagcgaca ggttgtttat aagattgacg gatgggcctg
    13861 tcatactccc gtagtattcc gacctgtagt atgaatccac tgcttcccgg ttcttttcat
    13921 cgaacctgcg gatgatcgtc ttttcctggt tgtagacctt tatagttttc tgacctgtta
    13981 tgatctcttc gacaaatccg ctcagttcgc ccagcttggc cgagcgcagc cggaacagcg
    14041 ggcgcacttt gccggtcata tatttcgtga gcaatatcga ggtcgggacc gttattgcaa
    14101 acaccagcac caggacaggc gaaatatata tcatcattat gagcgaaccg attacggtga
    14161 aaaggctcgt gaaaatctgg agcagatcat tggaaagtga agcgttgacc gtatctatgt
    14221 cataggagat acggctgatg atctctcccg gctggtggct gtcgaaaaac cgtaccggca
    14281 ggtccagcag cttgttgaat acatcgctcc tcatctggaa aatcaccttc tgggtgaggc
    14341 ggatcatgag gaccgacagt atatatgaca atatcgatga taatatgtaa aatgcggcca
    14401 tcagggcgca gtaataaaat actgtttcaa aatcgactgc gccgggcgtt gtaccgatcg
    14461 catctattgc gtttcccgaa agcagcgggc cgacaagagc cagcaggttg ctggatatcg
    14521 aaagcaccag cgcgagcagg ccgtaccatt tatagcggta caggtatttc cagagccttg
    14581 acaggatcct gagcgtatcc ctcatggtga acttccgttt ttcgccctgg gcctgtacat
    14641 taaatggatc tgagcttccg cctctctggc ggaagccgag actgccgggc ctgccgaaac
    14701 taaccgggcc ttgcaattgc ttcacctccc atctgtagtt cataaatctc cctgtatacc
    14761 tcacagttat caagaagttc ttcatgggtg ccatatccca gcattttgcc gtcttcaagg
    14821 accagtatgc ggcctgcatg catgatcgaa cttatgcgct gggcgattat gatggtggtg
    14881 gtatcgccat agttttccgc cagcgctttc ctcagcagtg aatcggtttt gtagtccagt
    14941 gcgcttgatg agtcgtccag tatcagtatc tcaggatcag cggccagcgc cctggcgatg
    15001 agcagccttt gcttctgccc gccgctgagg ttggaactct tcatcgcaag catatgatcg
    15061 tacctgagag gcagcgtgtc tatgaactcc ctggcctgtg cgcaggccgc agctttttcg
    15121 gtctgttcgg ggctgacgtc ccgcccgaat gcaatattcg aacctatggt gtctgcaaag
    15181 aggatgtcgt tctggaagac gacgccgaac tttgtatgca gttcatccgg cgggatgctg
    15241 cggatatcgt ctcctgaaat gcggatgacg ccttcgtcag ggtcgtacag cctcataagc
    15301 aggcggatga tggtgctctt gccgctcccg gtggctccga tgatcccaag tgtctcgccc
    15361 ttgcgcagcg caaagctgat atcttcgagg gtgttctgct tctcgttttg gatgccattg
    15421 ttccggctgc catcattcag gatgctttta attccatttg gtgtgctgtt gtcagcggca
    15481 ctcctgcctt tcttgccgcc ggagatattt cctgcactta aggctactcc tgcgttgctg
    15541 ttcgtatttt ctgtgttacc tgtgatattt cctgtgctgc cggagttgcc ttctgtactg
    15601 cctgagccgt ttccagcatt gacggaagct ttttctgcat tatcagaggc atctccggtg
    15661 ctatcagcgt cgtcttccgg cgttttggcc ctgtggtacg agaatgtcac atgctcgaat
    15721 gttatatgat acccgttgtt cacatggtct gcaggttcaa gcttaagatc ttcaggagcc
    15781 tccagcactt cgcggatcct gtcggccgat gcggcccctt ttgaatatat ctcaaacatc
    15841 cttgttattg aaagcatagc gtttagaatt atcgtgaagt aagtcaggaa tgctatgatc
    15901 tcgcccgatt ttgtcaggcc tgcgtttatc ctgaaagcgc cgacgacgat cacaagggtc
    15961 agcccgaggt taagcagcag gttcatggac gggttggtca gagccatcgt gaccccggct
    16021 ttcgtttcgt ttttcacgac ttcctggttt acttcgtgga aacgcttctt ttcatatgcg
    16081 gttttggaca gcgcttttat gacgcgtata cccgcaatgt tttcacgtac ggtgcggacc
    16141 agtttgtcta tggaccgctg ggattcggaa aacagcggga tgcctttctt tgatataagc
    16201 cagaccagca gggtgataaa cggcagcatg atgatcaggg tgagcgataa cacgggctca
    16261 agggcaagag tgactattat accgcccagc agcaggatcg gcgcacggac gccgaggcgc
    16321 tgcatcatgc cgatcatccg ctggatatgg tatgtgtcat ttgtcagcct cgactgcagg
    16381 gacgggatgg tatattcgtc tacctgcctg caggacatgt acgatatttt cacaaacagg
    16441 tcgtgccgga tcgcttccgt ggcgtcacgg gcgacttttg aagccatcct gtttgcaatg
    16501 acatttgtga tgaatgcgaa tgccgcacag aggaacatga tgccgcccca tatgaaaatg
    16561 agcctgacat cgcgaagggg tacgatgtca tctatcgtat gggaaaggat ccagggtatc
    16621 agaagatcca ttatcgtgcc ggtgaacttg ataacgaggc caaggagcat tctcgggaca
    16681 taaggcctca gatacgaaaa gaccgttttc ataggccagc ttcacttccc acgtttccag
    16741 cttcacatat ggtgattaca aaactctttt cataataacg ttttagatgc aaatttgtca
    16801 atgaaactgc tccggccgtt tgcgcccgtt gttacggccc tgctgttatt tccggggcac
    16861 gagaggcgat aagtatacct taatggtgag atcgatgtac ggcaggacag gcagccgcaa
    16921 aaacccaaag aagatgacat ctctgacaga tggtcaatcc tgtctttttt tgagataata
    16981 tcagggaata aaaaacagaa aggtgatcga atgagagcgg ttatccaaag ggtaaagaga
    17041 gccgccgtcg tgatcgacgg aagcccggta tcggagatat cgaaagggct gcttatactg
    17101 gtcgcggtcg gcagcaacga tacgcagtca gatgccgatt atctggctga aaagactgcc
    17161 gaactcagga tattcgaaga tgctgacggg aagatgaacc tgtccgtaaa ggatgtgaag
    17221 ggagaactgc tggcggtttc gcagttcacg ctgtatggtg actgcagaag aggcagaagg
    17281 ccttctttta cggattctgc gcgtccggag acggctcgtc cgctgtatga atattttgtt
    17341 gaaaaattaa aacaactggc agggactgac gtgaaaacag gggtttttgc agcgcacatg
    17401 gaagtggaac tggtcaatga cggacctgtt acgatattgc tggacagcgg gaaactattc
    17461 taggacatca aaaaccacaa cggagatatc cttctgcaga tcattcagct tatgcagaag
    17521 ggatccgcca taataaaaac cataaggagg agagttgata tggcaggcat tataggtacc
    17581 aatgttgtaa cacagatagg gttcatagtc agggacatcg aaaaatccaa gaggaagttc
    17641 gcggagtttt tgggcgtcga gccgccgccg catttcgacg ggggcaggtt cgaagtgaca
    17701 ggcaccaccg tggaaggcaa acctgcgccg gatgcaaact gcctcctggc ctttttcgat
    17761 gtgggtccca acttccagat agaacttatc cagcccaatg gcgccaaaag cacctggcag
    17821 gatttcctgg accagcacgg cgaggggata caccatatag cgttcaacgt gaaggatatg
    17881 gacggtaagg tgagagcctg cgaggatttt ggcatgaagt gcctgcagag agggaagtat
    17941 ggcgacggaa gcggcgaata tgcatatatg gatgcttaca atgacctgaa gtgcatcatc
    18001 gaacttcttg agagctattg acggcacaga ggggatgcat tgagggcaca aatgctgctc
    18061 catgggacga cccggccatc ctggccgaat gcccggggaa ggtatttgat ctgtacggag
    18121 gatgcatgcc tgaaataaag ccgggcggta tggaggcgtc aggcagccga cggcagtgcg
    18181 tatatacgcg gttgcccgca ccggggtgtg actgataaag ctgacttgtt gaaatataat
    18241 gattttttgg tgtataatat ataacacgat gtgatcacat gtgctaacgt gatttgcttt
    18301 atatggtttt cagccgtgaa ggaagtaatg gcccgtgatt cttcttaaag agagccggtg
    18361 gctggtgcga accggcagaa ggcaggatct ttccgcttcc ggagctgccg gtgatgagcc
    18421 gggggccggt accctttaaa gtaccagcga gaggtcggtc ctgtggaccg gcaatttggg
    18481 tggcaacgcg gataccttcc gtcccatggt tttacatggg gcaggaaggt tttttaatac
    18541 cggcgccggt gcactgacgc gccaatgtgc cggtttgcaa tgcagaaagg gaactgagac
    18601 gattgatcga aataagacag gaggtaagca tatgatcgat atcaggctga tacgcacgga
    18661 acctgaacgg gtaaaggaaa acatgcggaa aaaattccag catgagaagg ttggactggt
    18721 ggacgaggtc ctggaactgg acaggcagtt cagggaaaca aagtcgcgct gcgacgaact
    18781 caggagccag cgcaactcca tcagcaaggg aatcggcgcc cttatggcaa aagggcagaa
    18841 ggaggaagcg gaaaagaaca aggcgagggt cgtcgagatc aacaacgagc ttgaggagtt
    18901 ggcaaggctg gaggaaaagc ttgagaagga catcagggaa aggatgctgc tgattcccaa
    18961 cataatcgac gattccgtgc cgatcggcag ggacgacagc gaaaatgtag agatagagcg
    19021 ctacggagag ccaaaagtgc ctgattttga gataccgtat catgttgata tcatggagaa
    19081 gctgcgcggt atcgacctgg acagcgcgag aaaaaccagc ggcaacggtt tttattacct
    19141 ctgcggagat atagcgaggc tgcattcggc aatactgacc tatgcccgcg acttcatgat
    19201 agacagggga ttcacttatt acatccctcc attcatgatc cgcagcgaag tcgtcaccgg
    19261 cgtcatgagc tttactgaaa tggagaacat gatgtacaag atcgagggcg aagacctgta
    19321 tctgatcggt acaagtgagc actccatgat cggcaaattc attgatacga tcctcgatga
    19381 ggaccaactg ccgcaggcgc tgaccagcta ttccccctgc ttccgcaaag aggtgggcgc
    19441 ccacggtata gaggaacgcg gcgtgtaccg catccaccag ttcgagaagc aggaaatggt
    19501 cgttgtctgc aagccggaag agagcatgga atggttcgat aaactttacc atatcacggt
    19561 ggacttcttc cggacgctgg acatcccggt aagggtcata gagtgctgct cgggagacct
    19621 ggccgacctg aaggtcaaga gcgtggatgt cgaggcatgg tcgcctcgcc agcagaaata
    19681 ctttgaagtg ggaagctgct ccaacctcgg agacgcgcag gcccgccgcc tgggcatccg
    19741 tgtgcgcagc agggaaaaag gcaactacca tccgcatact ctgaacaata ctgtcgttgc
    19801 tccgccgcgc atgctcatag cattcctcga gaacaacctg aatgcggacg gtactatccg
    19861 tatcccggaa ccgcttcgta tatacatggg cggaaaagaa ctgataaagt aaaaacaggg
    19921 taaggcttga cactcaggta agacaggggg gacggttaaa aggtaagggg acactcctca
    19981 acaaggggta ttccttagcg gctgaggaat gtccccattc ctgggggaac tgtcctcgtg
    20041 tcttgcggtc ttgagcgtca ccaggcgtga ccggagaacc gtcccgcctg tccggcagat
    20101 agccggctgt cgtgcctggt acccggaaaa ctggaggtca ttatgcccgg gtcgatgata
    20161 cacctgctta tggcgcataa aatcaatccg gacagcagca cattgtttta cattgggaat
    20221 cttgcgccgg atgcagtaac tgcctggaaa gaaaaagata tcacccactt ccgcaacctg
    20281 ccggacagaa gcaaggctct ggccgaactg gcggcacgca cacctccgga cgacgatttt
    20341 gcggagggga tattgctgca cctgtacacg gactggcggt gggacactct tgcaagggat
    20401 gagttcataa agactgccgg tgacgggtgg ttcccgcaat atcgccatga actgagcctt
    20461 gccagtattt ttgcatttca ccggactgac tgggctgcgg atgtcatgga caggatctat
    20521 caatgcgaca aatcggaata tggcgaaata cccggcgcta ctgccgatga tctgagagat
    20581 ttcatcagcc atgtttacga gtggtatacg gaaactgaaa cagaggcgtc atccgtattc
    20641 acaccggaat tcgtcgaaag atttatcgat gagatcgcgc ctgaatatct ccggtggcga
    20701 agtgagcgcc cctatatttc aacaaaccta tgacaccgat gagttgatcg gtaaaaggca
    20761 atgggcggtc aatttcttaa gaggtattac tcatgagtgt cgtttgcctg gccgattagt
    20821 ttttccctgc ctgcaatatt ttttattatc ctgcctaatg cgtcgccgct cagaaaagga
    20881 gccaaactgg taaagattcc cgtatccagc ttatccttcg gtatttttcc gatagccttc
    20941 aaaatcccgt cttcaccaag aaatggcgcc agaggtacta ttatatccgg actgatgttt
    21001 ccgtcagcta tcttgtcgat caatctgctg aaagatctac cgctgatgaa aggggcaagg
    21061 ctgatgagca gattctcatc aagtttgtct gaggcagctt tgtctataat attgttaaaa
    21121 tctttttcat tcagaaatgg ggctatcgct ggcagaatcc cagtatcgaa ggttccgtca
    21181 gccgctttgt caaccagctt actgagtgtt ttaccgccca ggaagggagc aagaccgggc
    21241 agtacatgac agtctacttt tccttctggt atctggtcga ccagcctgtc gagatcatcc
    21301 tggccaagga acggcgcgat gccgacgagg tgctgtgcat cgacggagcc ttccatgaca
    21361 tggtccacaa gcccggcaag gacgtctttg ctcaaaaagg gcgcaagacc gatcagttct
    21421 tcgatatcga tgccatccag tttctctgcc acttcatcca atatgcttgg ctttatcgca
    21481 ggcgcaaggt cgaccacatc ctgtacgtcc gcttcgcctg cattgatcat ttgtgcgaca
    21541 ctgtccgtgt cgttcccggc gatccgttcg gcaaccgtac cgatactttt cctctgttcg
    21601 ccgggatcgc ctcccttaag gatattatct atgcttacgc cgaatatttc gctaagcttc
    21661 ggcaacaggc cgatgtccgg catcgattcg cccctctccc atttggatac tgcctgatgg
    21721 ctcacattca gcatatcggc cagttcgacc tgtgtcatat ccttctgttt gcggagaccc
    21781 gatatatacg ttccgacttt tctcatatcg atcatgattt tcactcctgg aaatcatatt
    21841 tgccagcgcg caccgaccag tttcaggata tcatggcggc aaaaacaggt caatcaatcg
    21901 ctggttgcag aatccggtat ggacggatct cgtgcctggt ccccggtcag tctgcaccca
    21961 ctgccgggga gacaagtcgt atttacattg aaaacagcaa acaaataagc aaaaatcaaa
    22021 gtatgatata atgaagcttg attgtttgca aattcgatcg gaaggacagt tggtgataag
    22081 cacatgtaca acgcgttttt tggcattttt cctatacttt tcagctttac gtttgtaata
    22141 gtctttatcc tcattgcttt ggcagcgatc cggggtattg cggaatggaa caggaacaac
    22201 cactcccccg tacttacggt agacaccagg gtagtggcca aaaggacaag tgtatcgcat
    22261 caccaccatg gcgggaatgt ggaccattac agtaccagta cgacgtacta tgtgacattc
    22321 gagtttgaaa gcggcgacag gctggaattg cgcgttccgt atgacgagtt cgggtatctc
    22381 gtggagggcg acatgggcag gctgactttc cagggcacac gattcaaagg cttcgagagg
    22441 ttcggagagc cggtatgatc tggtgccggg tttcctattg caatcttttg tcatcttgtg
    22501 gtatcatatg catgttatca ctgctgagag gcagaaacga aaggtgtacg gttttatgac
    22561 tgcatttgac aatgcatcag gatcataaaa ctgaatagtg gcagagcaag atacccgttt
    22621 gcgtatgaaa aacaggcggt cggcgttgcc gttcgccaac atcgatgcaa agggccggtg
    22681 cccgttactg ttggggcgct ttattttgct atgccgtatc gggtagcctt tcgtgaatac
    22741 cgcagcatat tcggagcaca tcggaagggt gtgctttttc atatatcggc aagttgatcc
    22801 gtttatacat ccacgtatac gcatttgcgc attgaccgcg gtttgcagca ttgagtgaag
    22861 gctgctttcc gcggttttta tttataaccg gaatattcta actttgaaat cgaaaggagt
    22921 tgtgtttatg agtgcggctt tttgcaaccg aacaacgaaa actgcaaaaa aagcggcaga
    22981 atttaatctg atgatggagg tagttgaact tgaaccttat agattatgga ttttccccgg
    23041 caatgatgcc ggaaaacgaa agcgggatac cggcccgggt catatcggta cacagggaac
    23101 gttatgaact tgtgtgcgaa catgggacag tttatggcgt gctaaaaccg gcagtatatt
    23161 tccgcagcgg gtcggaaagt ttccctgccg tgggtgattt cgtactgata aattacaatc
    23221 cgcacggtga cagccagatc acgaaaacgc ttgagcggaa atcgttcttt tcgaggcgcg
    23281 atccctcgcc cggccgcggt gaacaggcag ttgcagccaa ctttgactat gtctttgtca
    23341 tgcagtcgct caatgacgac ttcaacgtca ggaggatcga gagatatgtg accctcgcat
    23401 ggcagtcagg cgggatacct gttgtcatac tgaacaaggc agacctggcc ggtgacttta
    23461 ctgaccgggt cagggcggcg gaaaaagctg cagggaatgc agcggtatat gcggtaagtg
    23521 tgaagacagg atatgggctc gatgccatgg gagaatacct gaaaccaaga aaaacaacgg
    23581 ttttgctggg ttcgtccggc gtgggcaaat cgagtttcgt caatgcattg gccggaagtg
    23641 atataatggc ggtgaatggg atacgcgagt cagattcaaa agggcgtcat actactacgc
    23701 accgccagtt gatcatgctt gacagcgggg cgatgatcat cgacactccc ggaatgcgtg
    23761 aactgggaat gtgggatgtc agcaaaggtc ttggtgaagc attcgacgat gtggagcgct
    23821 ggctgggact ttgtaaattc agcgactgcc ggcatcagac cgaacccggc tgtgcgatca
    23881 gagaggcgat cgcaaaacac gaactgtcgc gggaacgctg ggagagctat ttgaagctga
    23941 agcgggaagc aagatatgcc gataacaagg atggctatct gcagaataaa aagagatggg
    24001 agaaggatat tgcaaaatat caaaagcaga ggcagggcag caaggcggtc cagatacagg
    24061 gtgacgaacc ggatcaccgg aatacaggct gcaccgagag ttttacctgt aaagtctgcg
    24121 gcgcctttgt cgttccggag ggtgcaggca gccagcaccg caatcattgc ccgagatgcc
    24181 tgtcaagctt gcatgtcgac aatgagccgg gggatcgtgc atcgctgtgc aagggtatta
    24241 tggacccgat aggcgtttgg gtgcgaaaaa atggcgaatg ggcaataatc caccgctgtc
    24301 gggaatgtgg tgttctcagt tcaaacagga tagccgccga tgacaatcct gcgctgttga
    24361 tgtccatagc cgtcaagccg ctggcttcac cgccgttccc gttggcacat ctggaacggg
    24421 atttttacaa cggccggcac tgctttgata aagcaggggg tatatcgtga ccggcaaatg
    24481 atttatatga ctgatacctg atgcctgaag cgaatgaagg agttgaaccg ctttatgaag
    24541 taccggattt tgtcgatccg cgataacccc gaatactcgg agcgggcaat agactggttt
    24601 gcctccaagt gggggatcga ccgcctggaa tatgaaaaga gcttcaatga ctgtctcact
    24661 aaaaatgaaa gccttcccca gtggtattta gcaattggag aaaatgatga gatcatcggg
    24721 ggctgtgggc ttatagagaa tgattttgtc gacaggacag acctgtttcc atacctatgc
    24781 gccctctatg tcgaggaaaa ggccaggagc cacgccttag gctcgaagct gctggagtac
    24841 gccaggaccg acgctgggcg gctgggtttt gacaaactgt acctaccgga cgtatgacga
    24901 caacgggatt ttcggtattc gtaccgtaac cggtactaat aataaaagcc tgataaactc
    24961 aggctttttt attgtggcgg agagggactt atggccctgt gcctacaccg ctgattctcc
    25021 agccgccttt ctccgtttct ttcgtcaaaa gaatgaaacg ttgctgtaca ccgctttccg
    25081 acgtaataac ctttttaaag ttaaagtcaa ccgttgcccg gtattcgatg accccatccg
    25141 gattttctat gtctttcaat tcactcatat caatcagttt tactgatttt acatttgagt
    25201 agccatctga atatacgccg gcagaatcgc tgttatagag cagattattg tccatgtttg
    25261 cgaagagata actgcacaga ctctctcggg ttatacaagc gagcagcatt ttttcatctt
    25321 gttcatccat tgcttcaaaa tactgccgga tcaattcctc agggctcatt gatgacaaca
    25381 ttgtttcaat ttcatcggaa tagtcgataa atccgtcgat ccaattaccc cagtctttgc
    25441 cggtcacatc cttcaaagac tttctgtcca gagagcaggc aaacccgcga tgcctccccg
    25501 catcaatgta tgcacctatg atttctccgt tgtgctttat aacaacaccc ctcgcgtcac
    25561 gtctgggttt cagagaatcg gggagcggtt cccgcagttt gtatatctcc gcagtggcgt
    25621 tttttccgag ataactgctg aaatccaggc ccacgctttt ggaaagctca ttattatagg
    25681 cccaataaat ctttagcgga aaatctccgg cttgatgttt aaggtctgat ggtaaagtga
    25741 cttttacgct attgaccagg taatctgctg tccagccgta tttgctgaac agatctgaaa
    25801 ccttactgtc aatgtaggtc tcgtgccgcc tgtattcaac atagtcatcc aaatacctga
    25861 agaagtcata gcctgggtct aataactttt catcaatctg aacatacccc aattggtaaa
    25921 ggtcatcgta tgcgaacatg aattcctttt tgcttccgtc tttaagtgtc agaaccaact
    25981 tgttgctgcc tttggtcatt ggccgtatct tatccaactg atcgtccgaa aatgcctcac
    26041 ttcgtgaaat catatccatg atatccgaaa tgatcttgct gtctttaatt tgtacaggat
    26101 cgatttcaat gcctggataa taggtattgt cataataaat gccgatctgg gtgatttccc
    26161 cgtatatggc ctgtttatcc gattcattcg acccgggact gcttccgtca acatgctcgg
    26221 ttaccggtcc attatcagct ccatcctcac tgtgctgcgg aatgatctca gcattttcgg
    26281 attttgctcc cgtgaaggtg cagccgaccg caacggcggc gacgagcgcg gccgcgacca
    26341 atgccggcac gaggactttt ggctttctcg cgatcaatgt gatgcgttcc ctgataccct
    26401 ttattccgtc ggtcatggtg gtggcgcagc aaagcagatc catggttttg cgtttcccgc
    26461 aggtgagtcc gatgatcgtc cgtccgtatt ccaggcggct tgcttcgcca aggcgtttga
    26521 tggttccctc atcgcaggcc agttcggaat ccctgcggga aagtgccgcc gccagccaga
    26581 caagcgggtt gtaccagtgt acggcgaggc agaaacagcg catcgctgcc catatgtggt
    26641 cgccgtgccg gtaatgggtc tgttcgtggg ccagcacatg gcgcaatttt agagcatccc
    26701 cggcaacgtc cggagtgata tagattacag gacgaaagaa tccgaacagg cagggcgatg
    26761 ctaaattctt aactaaatag atcggtcgct tacaattgtc tgactgatag cggatcctga
    26821 tttttctcag tttcctgctg aaactcaggt tcgataaaag caaggtcagt ccgacaacgg
    26881 caattccggc aagccatact gttctcagca tccgggccag taccacacca agaggatcca
    26941 caaaaacata tgtctgcggg tgtgaataac cgctatttga tgggtacccc cgaatctcat
    27001 gcggcgtgcc ttttccggct tcacgcgcct catccggcgt catttcactg ttatgtatca
    27061 cgtcagagta taccagagtt tttgaaagcg tctgttcagc cactttgtag gccgccgacc
    27121 gttcaactgc attcattaca ctaattgggc tctcaaacaa ggtaatgggt acaatcaaac
    27181 gtatcagcac cagtgcccaa agggcgtatt gcaggtgcaa actgattttg ccctttaata
    27241 aatgccgcaa tattattacc acaaggataa gaacggagga agtgatgatg agttctgtca
    27301 tttctccccc tccttgactt cagcttcacg caggatggcg tacaattctt cgatttcttc
    27361 ctttgtgagt tcctgcttct ttgtgatggc gctcatcatc aggctgacgc tgcctttgta
    27421 taccctgttg aggaaatcgt ccgtttccct gataaccgcg tcctcccgct taatgagggg
    27481 agagtagacc tttattcctt cattgtcctc gcatctgatt aagccctttt ttgtcatacg
    27541 tccaagcatc gtcagagtcg tgctccggga ccagcccaca tgtttgcgca tatattccgt
    27601 tgcttctctg ccggttcgcg gcgacgcttc ccacaggcat tccatgagat tccattcggc
    27661 attggtaagg ctaatattct ccacattcat cttatcacgc tccacgctgt ctacaatgta
    27721 aacatattat atgccaatgt gtttacattg tcaacactat gcagggcact tcgcctttga
    27781 ctttacagtc tcaaaagtgt gttgatatct gctgcaaatt cgtcagaaaa atctcggcta
    27841 tcgaataaaa cggtgctaca taagctccgc aaataaaaag aacttatcac actttaagcg
    27901 taataagttc ttggcggaga gggcgggatt cgaacccgcg tgtcgtttcc gacaaactga
    27961 tttcgagtca gccccgttat gaccgcttcg atacctctcc atatattgta catctataag
    28021 attttgctta tgtttatctc aggcttttaa agaattcctt caatatcgtc gagcattcct
    28081 gctccagcac gccccagacg acgtcgaccc gatggttgaa ggcatccacc ctgaacacgt
    28141 ctatcactga gccggcggcc ccggccttca gatcggacgt cccgatgtac agggtcctga
    28201 tcctcgactg tatgatcgcg cctgcgcaca tcgtgcaggg ttccagcgta acataaagat
    28261 cacagtctgt caatctccat gtgccaagct ttttgcaggc acgcctgatg gctgtcattt
    28321 ctgcgtggag tgtggagtcg tttttcgact ctctttcatt gtggcccctg gcgattatta
    28381 cgccgttcct gactactacc gctccaaccg gcgcttccgc ttttttcctg gctttctccg
    28441 cctccatcag cgcttcggcc atgaaccttt cgtgcgtgaa aagctgacag gccggcttgt
    28501 catgcgccat gtcccaacct ccgcataaac ggaataaatg cgcacacgtt attatactac
    28561 aaagatgaat aacaatcaat tatcttaata ttacattatt tggacaatgg atgtatcatg
    28621 gccgtccgtt tggccagtgc cggtatgtca ggctatctgt gcaggcattg cccgatatat
    28681 catggccgct tatgcaggca atgcacgaag ggacagatac ctgtacaaaa tacaggaatc
    28741 ctggcggaga tcagatatct cagccgggag cagctatttc accttgaagc atatttgccg
    28801 ctcaggttgt atatgatcct gaacgggaga aatataaggt acagtataca tatgatgaga
    28861 aactccatga tcatgtctat gaatttgctg aacctttcca taatatcacc cgcctggtat
    28921 gggagaatag tggctttgca ggctgaaaag cctgttctgc gctgatcggc cgacattatt
    28981 attggcttaa atggcaggtt ttatgtaatt gccctgatca catgcgtaaa aaatcgatta
    29041 ttgcccgtac tttcacggta ccgggaccgg ccgggaaggt aaaaggtacc gcggtgtcgt
    29101 ataatgtaat ggggtgatat tcttgagcgg acttattttc atagtgtttc ttgtaattgt
    29161 tatcataaat atattcaaaa aagcaagtga aagcggctat aatggcagcg gtgaacataa
    29221 aaggttcgaa cggtatctcg aagataacag gcagaacagg cccgaaataa ggaatatcga
    29281 gcagaatgag cgtaccaggt ggtatgggag cagttataaa agcaatgccg gaaaaggcga
    29341 tgcccaaaga cagaatgggc aaagacggag cacacaggag gcaaattcct ggcagacagg
    29401 ccaaagcacg caggaaaccg ccgccggtca aaccagacaa agtatggcag acatggccgt
    29461 caaaccgaat gaaccttgcg atccgaacac agccatcatg aaaaacggag ccatcggtac
    29521 caaagctgcc gatacgaatg cagccgataa aaccaaagct gcgtacatac cggctgcaac
    29581 cggcagtttt ctttccaggg ctgaagacat ggacaaagtt ttcggcgata cctccatgga
    29641 cgtgccgatc actgcagacc agggttatac cgtcactgaa tgttatggcg taaatgatac
    29701 tgagagttat agtgcatctg acatgcaata cgaggggtat ccgcaggaca gctttgaagc
    29761 aggcaatagt atactgacgg attactaccg ggacattcct gttgcggcgc aaccggtttt
    29821 cgtgaaagat gccggcagcg agggcattat tggcaccgga gcgctggttt ggccggaagt
    29881 aaatgaaaaa gattatttta tgtccgacct gaaagctttc gaatttacgg atgtcgtagt
    29941 ggattcccaa ataaagctgg atgcgtcgat gcctttgttt gaggagatta ataaagctcc
    30001 ggtaaaaaca gttgataaaa acgatggtac ggctggcaaa gccgaatagc cgaaaattga
    30061 ctgatgatca gctttagtca gcggtatctc gtatttgctg catttgcgcc gatattgcac
    30121 catatctgca gcaggaactg tgcttgcgat agcaaaagga gcctgtgcgc aaccatacag
    30181 gctcctttta tataaatacc ctgcccggat cgacctggcg ttgatatatg ataccgggtc
    30241 accggatcat acctggcctg aaagatcatc cttaactgat tgatcgctcc atcattgccc
    30301 gattacccaa ggatcgcttt cagatcttct tcaggagtag atatcggctt gataccgaac
    30361 ttatcgacca gcacattcag cacgttggat gagaaaaatg ccgggagtga cgggccgata
    30421 tagatatttt tgagaccgag cgccagcagt gtcagcagga tgcagacggc cttctgctca
    30481 taccatgaca gcacaagcgt cagcgggagt tcgttcacgg tgcagttgaa cgcctctgca
    30541 agagctgtcg ctacctgtat tgcggagtag gcatcattgc actggcccat atccatgatc
    30601 ctcggcaggc cgccgatctc gccaaggtca aggtcgttga agcggtactt tccgcatgcg
    30661 agcgtcagga tcaccgtatc cttcggcgta ttcttcacaa actccgtgta atagttcctt
    30721 cccggttttg ctccgtcgca gccgcctaca aggaagaagt gcctgatggc tccgctcttc
    30781 acggccttta taacggtgtc ggcagcgcca agaacagtgt tgtgtgcgaa gccggtcatc
    30841 acccacgaac cgccgtttat gcccgtgaag tgcttatcct caggccatcc gcccagttcg
    30901 accgctttat ttataacagg agtgaaatcc tttctgccgt cggttccagg tatatggaca
    30961 agtcccgggt atgcgaccgc tgcggtcgta aacaccctgt cggcatagga cggcttcgga
    31021 ggcatgaggc agttggtggt gaacaggaat gctcccggca cgtcagcgaa ttccttctgc
    31081 tggttctgcc atgccgtacc gaagttgccc ttcaggtggg agtatttctt aatttccggg
    31141 tacccgtggg ccggcagcat ctcgccgtgg gtgtatatgt tgatcccctt gccttcggtc
    31201 tgttcgagca actgcttcag atcatgcagg tcgtggccgc tgacaactat aaacggtccc
    31261 ttctccacag taagcgatac cctcgtcggc acaggatgcc cgaattcgcc ggtgtttgcg
    31321 ccgtccagca acgccatgca tctgaggttg acatgcccga actccataag gagatccagc
    31381 cattcatcga ctgaatgttc ctggccgacg gctctcattc ccttgtagaa ccatgccatg
    31441 acctccctgt cttccctgcc gagtacaaaa gcatggtatg catatgctgc catacccctc
    31501 agaccaagca ggagagtgga acgcagggat acgatatctt cagcgccggt ccagagctca
    31561 tttgccggaa aatcctccgc gccgccgagc cttgcctttt cttccctgac acgtctcgtc
    31621 acgtcgtcta cacggtcttt gtcgaagttg acattggtga tcgtggtaaa cagcccctcc
    31681 agcatcagtt catccgttct tccggccggc gctgttttct ccgccgccct ggcaaggcct
    31741 atcaatgcgc aggtcagttc gtcctggctg ttggccacct ccgcactttt tccgcagacc
    31801 cccgcttttg tgcatccctt tccgcctgcg gtctgttcac actggaaaca aaacatactg
    31861 cacatactgc tcattcaata ttccctttca taaatggatc aatgtatcat ttcagccatg
    31921 gtcatctatg ttgctttggc atgttcattg tagtataatc cgtgtatcaa atccgtaacc
    31981 aaagttacaa aaagttaata acaggtggac attatggaaa cgaagcatat agaaaaggta
    32041 caaagcatag ccaaagactg catgcttttc aaagatatga acggggaaga actgtctgcg
    32101 ctgctgacat gcctggattt caagatccgc agtttcagca ggaacgacat catcaatatg
    32161 gcaggcgact tcttcgaagg tatcggcgtc gtgatcgacg gacaggtggc tgtggtcaag
    32221 gagaacatag taggagagag gataatcttc gacatactcg gccgggggga gttgtttggt
    32281 gaaatggccg ccttcacagg ggtgcgcaaa tggcccgcaa ctatcatcgc caatacggaa
    32341 tgcacagtga tgcatgtacc ggccgataaa ctcgtcgggc agtgttccca gacctgtact
    32401 gcccatacga aactgataat gaacatgctc gggatactgt cacgcaaggc tctgacgctg
    32461 aaccgccaac tggaatacct ctccataaaa agcatcaggg gcaggatagc gagcttcctg
    32521 ctggagcaat ataagaaaag cgggaaaacg acctttatgc tggcgatgaa caggaatgag
    32581 ctggcagatt tcctgaacgt ggccagaccg tcactgtcac gggagatgtg caggatgcgg
    32641 gatgaaggga tgatcgactt ccacaggtcg tcggtacgga tcagggacat ggaagcactg
    32701 aaagctgcgg cacagggaat gcactgatcc ggtgtttatg ccccggcggc gggaaatggc
    32761 gcagccgccg aaaccgcgac gttttgattt gtactggttt agaaatactg aaataatggt
    32821 atgaataatg acaaggcgat aacagatgcc tccgcttcgc tgaaacgcgc agaggcaata
    32881 aaaatttctg cagacgaaat ttttatttga ttgatgcatt ataaaacagc ctgacataag
    32941 gattggaaag acttgcacct gaaaggccca ggtgcgaaca agaaatggaa gaataaggag
    33001 aatgcctatg aatacaaaaa caccttgcga atctatggtg gaaatgaccg aactggtact
    33061 gccaaacgat accaacctgc tcggcaacct gctgggcgga aggctcatgc atcttattga
    33121 cgtggcaggt gcgatggctg cacagaggca ctgcaaccgg gctgttgtga cagcctgcat
    33181 cgacagcgtc gatttcaggc atcctgtgaa gcagggcagg gccgtgatcc tcagggcaaa
    33241 agtgacctgg gtgggacgga cttcgatgga agtgcgcgta gacgtatttt ccgaggattt
    33301 cctgacaggg aagcgggagt tcaacagcaa tgcgtatctg acattcgtcg ccgtggattc
    33361 cgaaggcaaa cctgtggaag tttgcggcct tcgtcctgaa acagatgagg aaaaacggga
    33421 atacgaggct gc
//
LOCUS       DAOSCC010000003        31139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1076_length_31139_cov_4.834352, whole genome shotgun sequence.
ACCESSION   DAOSCC010000003 DAOSCC010000000
VERSION     DAOSCC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 31139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31139
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_31139_cov_4.834352"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLG72_00360"
     CDS             complement(<1..308)
                     /locus_tag="PLG72_00360"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062597.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOJ79288.1"
                     /translation="MYRYIPNTREDQEKMLSAIGAGSIDELFSDIPEDIRLDRELELP
                     AAMSEPELVSHMRAVASKNINAADHPCFLGAGVYDHFIPSVVWHVISRSEFYTSYTPY
                     "
     gene            complement(310..693)
                     /gene="gcvH"
                     /locus_tag="PLG72_00365"
     CDS             complement(310..693)
                     /gene="gcvH"
                     /locus_tag="PLG72_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOJ79289.1"
                     /translation="MEMYEGVKFTKEHEWAREEGGKVYIGISDYAQKALGDIVYVELP
                     GIGKKVKAGDPISVVESVKTASDIYSPVSGTVVEANEALEDSPELLNEDPYENYIAVI
                     DPDDLSELDELMDEEEYSEFCSEEE"
     gene            complement(750..1856)
                     /gene="gcvT"
                     /locus_tag="PLG72_00370"
     CDS             complement(750..1856)
                     /gene="gcvT"
                     /locus_tag="PLG72_00370"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ79290.1"
                     /translation="MENTIRKTPLYEAHVKAGGKMIDFHGWLLPVQYSSILKEHENVR
                     TKAGLFDVSHMGEIEISGSDAFEFLQHILTNDITAKPGKAVYSPMCYADGGTVDDLIV
                     YRQNGDSYLLVVNASNADKDFQWLRDNKKGNVDIKNRSDEYAQVALQGPESENILRSL
                     TTAGDAREILSDLKFYRYVNEMQIGDVKVMLSRTGYTGEDGFELYCRPDDALKLWDML
                     CDAGAAYGLMPAGLGARDTLRLEAGLPLYGQELSETISPVMAGLGRFVKPGKGDFIGR
                     EALVSQMEGNLAQRLAGFEMIDRAIPRTGYEVYRSGRKVGHVTSGGFFPTLKKNMGIA
                     LVETSCADDDTDLEVMVRDKKYAARTVKLPFYKR"
     gene            complement(2032..3042)
                     /locus_tag="PLG72_00375"
     CDS             complement(2032..3042)
                     /locus_tag="PLG72_00375"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOJ79291.1"
                     /translation="MAYDTRIIRTCSKDPWWNLAVEEYLLERVEPDQCILYIWQNENT
                     VVIGRNQNPWRECRTDLLESEGGKLARRLSGGGAVYHDSGNLNYTFVSGRELFDTERW
                     FGVILSAIKSLGIDAEKSGRNDLTAGGRKFSGNAFCIRKQSAYMHGTVLVSTDLDSMS
                     KYLQVSEEKMKSKGVKSVKSRVVNLSELKPGLSADNAAEALIESFRREYGSVADVEGT
                     LRKDRESREAIEELYCKYSSWAWRYGEAPDFDVTYETRFPWGGVEIGFTLQNAMVSKA
                     VVYSDAMDAAYIESLAEVMKGMRFNAEELADQIVEAAMEGPDDVRIMAYDLAGWLRSK
                     CF"
     gene            3249..3497
                     /locus_tag="PLG72_00380"
     CDS             3249..3497
                     /locus_tag="PLG72_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HOJ79292.1"
                     /translation="MRYQEDFTGTKTEFGDFIKKAIPELFAGRLTVEGKTISIPSDVT
                     LDYKVKYDEDAEGGSVSIKVSWDNPNLDLEIEEEEEEE"
     gene            3612..4643
                     /locus_tag="PLG72_00385"
     CDS             3612..4643
                     /locus_tag="PLG72_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HOJ79293.1"
                     /translation="MPPEVREPLEEVLKHYPLTVLDIRNESYKEKKGVWWVRTSSGMK
                     ILKKISNSEQTLRFTLDAVRHLRNNGVLLPEVNRTSDGNEYVKIEGTCFVLTDAVEGR
                     NPSYSSPEEMALIASGLADFHRASEGFRPSEGSKPKYHLGTWPEVYSEGLEDMRSYYD
                     SLLMKNSHDTIESIIVREFPYFYERGKKAVEGLKGNDYMSWSQEAESKGSLCHQDFAA
                     GNLLLTDRKKLYVIDTDSITIDIAARDIRKLLNKVMKKSGKWDPELASGIIRHYQARN
                     PLTPSQWEVVRLDLLFPHLFLGAVNKYFYKRDKEWSYEKYMKRIREMAAFERTAEPVL
                     ENFSRIVPV"
     gene            4681..5727
                     /locus_tag="PLG72_00390"
     CDS             4681..5727
                     /locus_tag="PLG72_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HOJ79294.1"
                     /translation="MDIVNKAEATGNLSIACDPAIAFNVLKEYGIVPDSVSVVQGGTV
                     KTVWKVSSGSTLYCLKRLKHTIDKALFSVNAQIHIKNSGGLVPGIIPNRRGEPITQYN
                     GQLFVLYEWIAGKDLDFNVPADLRTAVRGLARFHLASKGYQAAESARVSTKLGKWPEQ
                     YMSMSKKLSSWKELAERSSPSQAFSSYLQHVGRMLELTDKALKALNASDYTRLAGQGS
                     DRAVLCHQDYGKGNALAVGSSVYILDLDGVTWDLPARDLRKIIGKHAERHNRWSAEAI
                     SEILGWYTEINPMGAEEMEVLYADLLFPHWFYGLVKNLFQNGKTLKASEIEKTALLES
                     SKEEALRLCFKRSD"
     gene            5729..7642
                     /locus_tag="PLG72_00395"
     CDS             5729..7642
                     /locus_tag="PLG72_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ79295.1"
                     /translation="MKIALICTEKLPVPPVAGGAIQMYIEGILPYLSPHHDITVFCIQ
                     HPGLPEEEVRENVRYIRVPGKSQTAYIQNIAESIAASVDKDGTPFELIHVFNRPKFFL
                     ALSERFPGSRFGLSLHNEMMHAEKISDDAGLKCIEKAEFINAVSRYIAGTVTSRFPAA
                     ETKMRVVYSGVDPKKYDPPWTEAGAAKKLEIKSKYGLQDYRVVLYVGRLSIKKGVHIL
                     INAMEKVMQTHPDTALVIAGSKWYGSNAGDEYTDSIRKLVLKLPGPVVFTGFIPPSDI
                     AGYYCLGDIFVCASQWNEPLARVHYEAMAAGIPIITTDRGGNAEVMYGTDAAAAANAA
                     NPAGTAGGCGIVIDQYNDPAVFADKIRFLLDNPGVASSMGRKGRQLAEEKYNWKRVAS
                     EIFPFTGHDISRADMQQVNVHPDTGTGMFREGQVQVAARKPSADNPAVTANISSYAAT
                     LADESIQTAAAIPAAITHIAAGSPLSGTATAADTIAPGANAHTANISVIGTGYVGLVT
                     GACLASMGRKVICCDQDRKKIKDLSSGIVPIYEPSLKELVDSGIREKRLSFTDDIRQA
                     VGHSDIIFITVNTPTLPDGSCDTGNVFEAARSIGTCMDRYKMIVNKSTVPVGTGKSVK
                     QVISAMLSIRGGA"
     gene            7841..8791
                     /locus_tag="PLG72_00400"
     CDS             7841..8791
                     /locus_tag="PLG72_00400"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ79296.1"
                     /translation="MKVLVTGGAGFIGSHLCERLLRSGSEVICLDNFSDSYTPSVKRK
                     NLDCCVRCSHFRLVKGDILDPEVTDAVMEQEAPETIVHLAALAGVRGSVLDPLSYVDA
                     DVKGTVAMLECCRKFGVKKFIFASSSSVYGNSGTPFMEENTPPVQVSPYAAAKYSGEL
                     FCRTYSHLYGLPVVCLRFFTVYGPRQRPDMAVHTFARAIVEGSEIKIFGDGSSTRDYT
                     YVDDVIDGIMSSIDLPCSYETINLGNSVTTSILELVGILESKLGKKAKLSFLPAQPGD
                     VPATCADITKARALLGYDPKVPLEEGIERFVEWYLKRVAG"
     gene            complement(8979..9152)
                     /locus_tag="PLG72_00405"
     CDS             complement(8979..9152)
                     /locus_tag="PLG72_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79297.1"
                     /translation="MITKKIVWEEGMAKRCLDRVTRGYVPAYVHEEKNLTRSLKTGEW
                     DDFRRKISKEMKK"
     gene            complement(9237..10205)
                     /locus_tag="PLG72_00410"
     CDS             complement(9237..10205)
                     /locus_tag="PLG72_00410"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOJ79298.1"
                     /translation="MSGKRFTIAAAAIILLAGGSVLAVSLTGVNNAAKHLELGNGHLL
                     EGKYEEAIRAFEKAIRIDPENIGARMALAEAFIMTGDPGNAEICLKEVLELEPGMAEA
                     GIRLAEVYEKKGETGKAISLLEEILKAEAENIDNNVYIKLADLKIKARDLDGAILLLE
                     EAYQKTNSKRIMEKLDELRPASQKESRAVSEPAPELTESPEPASEPEEILAPAQTDIT
                     APAQAETPGPTPANASIERGNTVGNLANNGLAAMQGDWIYYYDYYDGGKIYKIRTDGS
                     VRTKVNDDDSGDINVVGDWIYYRNYDDSGRTYKICTDGSGRAIVGK"
     gene            complement(10544..11950)
                     /gene="cysS"
                     /locus_tag="PLG72_00415"
     CDS             complement(10544..11950)
                     /gene="cysS"
                     /locus_tag="PLG72_00415"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ79299.1"
                     /translation="MKLYNTLTRQKEEFRPIEGNTVKIYSCGPTVYQYSHIGNMRSFI
                     FMDILRRVLKYNGYKLKHVMNITDVGHLVSDADEGEDKMVKTARETQKSPWEIAEYYT
                     KVFMEHIAELNIDKPEIIPKATEHIKEMLEFVEGLVEKGYGYETSDGIYFDIMKFKGY
                     GKLSRLDLEGQKAGARVDVNTEKRHPADFALWKKAPKEHIMQWPSRWGMGYPGWHIEC
                     SAMARKYLGDEFDIHTGGVDHIPVHHENEIAQTEALLGKPAVRYWLHGEFLLVDNGKM
                     SKSLGNTYTVDDLKKKGYDPLAFRYMCLNANYRSKLNFTWDALDAAQTALERLLEGVL
                     AHKNADPEVKIPDEIIQGFENEFQDSINDDLNIPKAMAVAWNVVRYGTKSQDLYRLLL
                     RWDQVFALDLDKAAEKVKAKEETPELDPEIMALIEQRQQARKEKNWKVADEIRDRLNR
                     MGILLEDTPQGVRWSYKK"
     gene            complement(12072..12872)
                     /gene="cysE"
                     /locus_tag="PLG72_00420"
     CDS             complement(12072..12872)
                     /gene="cysE"
                     /locus_tag="PLG72_00420"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOJ79300.1"
                     /translation="MFRGLLSDARSIAKRDPAAKSTLEVVLLYPGFHVLIYHRIAHWL
                     YRKKVFFLARLVSQFARCVTGIEIHPGAEIGRGLFIDHGMGIVIGETAVVGDNCTIYH
                     GVTLGGTGKEKGKKRHPTVGNNVLIGAGAKLLGPITVGDNAMIGSGSVVLNDVPPNST
                     VAGVKARVVRQDGRKLAPSIELDQINIPDPVAQELCKIIERIEMLEAAAEGRSAAAID
                     RQACRERLRGQDADNRSQDSGTESRDSDSGKQDSGADAADTGTKPQDA"
     gene            13304..13666
                     /locus_tag="PLG72_00425"
     CDS             13304..13666
                     /locus_tag="PLG72_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOJ79301.1"
                     /translation="MAENVERSQRFSIGQLLVRWLVGAIVLAVTAFLTPGFTISGIWP
                     LIIAAAVIAILDYIVEMILGVDATPFGRGLTGFIVSAVIIYVVKFFVAGYNVTVLGAI
                     LGAVVYGIINLLIPGRTM"
     gene            complement(13883..14788)
                     /locus_tag="PLG72_00430"
     CDS             complement(13883..14788)
                     /locus_tag="PLG72_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79302.1"
                     /translation="MGKHDKVLPGLISERDLCKIREAVCIQVEKIYDSCKEKDCIEDA
                     RVFFKNPDKVNWLVNNAINVKARRAEVVKVFSDVEEVPFKRGFFTVDIKFFIKVTLDF
                     FVPRRNGGIRVITVPGIVMFDKKVILFGSEGNVKIFKSHFADKGLDDQGSSALQQDNL
                     PISKIEVAEPITLNAKIVEVLENACDDGNDRGIPATVSDLLEDVDFDDTDLLRGEERH
                     LTIGKRVVATIGLFSIVKLARYVQLLIPAFDFCVPNKQCVAATDEDACGLFDTIEFPI
                     DEFFPPQKADFPGAEEDTRSVAEEE"
     gene            14957..16660
                     /locus_tag="PLG72_00435"
     CDS             14957..16660
                     /locus_tag="PLG72_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79303.1"
                     /translation="MFNFHNRHFIFRHADGLVRDFYCDHRQSICCSILGRNGIWSDVS
                     IIARDAHQYFYAELGRDNFYHILYQDNSGNINYSKTDGQYSKTFPVLASKTPAVYNKH
                     LYIAPLDDEIYLFYVLQHENSFLLAYQTVKNNRPGTPKIVDYVSGNSIPCAVLYDSDQ
                     NIYAFYQSYDGKYLQLGYKKFSTVRKHWSDFTAVTRYQGNCEYPHATMDPSGTIHLCY
                     QRRAPKLFELVYQQKAPDKNLWSAETVLHSSVHPFENASILHVNGRIIVYWVRNDVIY
                     WCAGSPAGDGWTRPARYGYQPGHRLQCVCFKDLLAGDSPGQGDGSSLSLSPGIFPGVV
                     SDGFNLAFMDTGDITGGQPFQQIPPPSAAATGDTSGSELKSLVLSIFKEMQDRISEMK
                     TEWEEIKKEMPGLTSAYMELAKETEKLSIRLNMLENRLAQINSQYRRTEPSPGTVKGS
                     VQPAADRFEPTSAQGAPTAESKHPASVSEQAPVSVHGSAQAVQGGPVTTSFKDVTHNG
                     MKPSSTAKDHPAADDTAGTVPSETASHEAPAGRPKISPEPEWLKIWEEWKEPKEWSEG
                     G"
     gene            complement(16703..16914)
                     /gene="ssrS"
                     /locus_tag="PLG72_00440"
     ncRNA           complement(16703..16914)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLG72_00440"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     assembly_gap    17268..17277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17278..17470)
                     /locus_tag="PLG72_00445"
     CDS             complement(<17278..17470)
                     /locus_tag="PLG72_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOJ79304.1"
                     /translation="MYAVATLDSGNYALKLSSILEKKGYVFEVVPTPCQIAKGGCGYC
                     LKFPLEYKDIVLQEALFNKI"
     gene            complement(17544..18428)
                     /locus_tag="PLG72_00450"
     CDS             complement(17544..18428)
                     /locus_tag="PLG72_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOJ79305.1"
                     /translation="MHYKGYDIYEEGNGVRVEGIRDFDPVHIFECGQCFRWYREPDGS
                     YTGVVRGKVANVLYRPGVLELDNVTAEDFKQIWFDYFDLGRDYGLIKKAVTTDEIMEK
                     AVEFGRGIRILKQEPWETLISFILSANNRIPRIMKIISEISRLFGTELEYAGKPYYSF
                     PDAQTLADCSLEQISECRAGYRCGYIHETAAAVAESGFDTGRLAEMQTADARKELMKY
                     KGVGGKVADCVLLYSGARYDVFPVDVWVKRVMEELYFGREASFSEIHRFAAEKFGQYA
                     GFAQQYLFYYAREHRIGI"
     gene            18627..19406
                     /locus_tag="PLG72_00455"
     CDS             18627..19406
                     /locus_tag="PLG72_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOJ79306.1"
                     /translation="MKEKIYTIPVTEAFSEDCECPLCLLESRLEKEYIDYYLGPSLME
                     PDCRIDTNKKGFCRRHYELLYNRQENRLGLGLITDTHIREQLERLKDAAEPLLGGHGS
                     SRGPGFSFGSLASKFGPKAQTDSGPASKLLKLLTELENSCTICGRLEYTMDRYLDVVM
                     YLWAKESDFRKTFSEKKGFCLVHFRMLIQAAEKYLNADKRKEFLRILAQQQLEHMDRI
                     EKELEWFTKKFDYRYNDAPWGNSRDALPRSIQKLKGYCSLK"
     gene            complement(19410..20066)
                     /locus_tag="PLG72_00460"
     CDS             complement(19410..20066)
                     /locus_tag="PLG72_00460"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOJ79307.1"
                     /translation="MRQRSQYQGPSFQNQRQQFSYIRLMNAVPGLQPVDIYANRSLIA
                     QSLPYKGFTEYFQVVPGSYNMTVFAAGTTGPVLLDAVIEIPARSINTVALMGVPPALS
                     IRPFFETVVQVPPGRLYLRFGNLVPDGPDMDLTLSNGTELFTDVSFGMVTNYLSVPAG
                     TYIFYIKQSNTDTTLLYVPNIQLLEGRFYTIYTVGRMDGSVPLQVLIPLDGNSYIQVG
                     "
     gene            complement(20377..21198)
                     /locus_tag="PLG72_00465"
     CDS             complement(20377..21198)
                     /locus_tag="PLG72_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ79308.1"
                     /translation="MRVVGFTGASGTGKSHRASWVARERGIQYIIDDGLFIKENRVIA
                     GYSAKKEKTKVGSIKRALFTDDGHVDEVKRAIKQHQPEAIMILGTSEGMVDKIAERLG
                     LPEVSERIHIEDVADEFEIKQALATRREQGKHVIPVPAFEIKKDFSGYFLDPLQIFRR
                     KGKLSFQPDGEKSVVRPTFSYLGSYTISDYTIYQIVEFVVSGFSGVSRISRFRAENQP
                     DGIYMEMDLILKYGYPIRALVRELQQKVSEEIERLTALNIQQFNVTVKSLVVEKQ"
     gene            complement(21615..22370)
                     /locus_tag="PLG72_00470"
     CDS             complement(21615..22370)
                     /locus_tag="PLG72_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79309.1"
                     /translation="MKFIQEPNLPEGKVGLAVVDGRISRKAENALRALGIELVRLRPH
                     PGLYEAVSCHPDMMLHHIGENMIVYAPGTDAEAVSKLDAYGFRMVMGQSALMSKYPYD
                     IAYNVARVGKWYFHNLKYTDPQIKAFMERLGIEPIHVEQGYSKCSILPVDENSIITTD
                     SGIERAAGKKGIDVLYLECGDSIRLPGLNHGFIGGACGMISGSVCAINGCIEKLKGFN
                     SMVSFLSERNKTVVQLSDECVTDIGSIIPLMLI"
     gene            22697..22861
                     /locus_tag="PLG72_00475"
     CDS             22697..22861
                     /locus_tag="PLG72_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HOJ79310.1"
                     /translation="MTTINCSLNCKHQKDGSCMLEDAASDTLSVDTDCVFFEEKHDKA
                     GSSASSRSSE"
     gene            22918..23151
                     /locus_tag="PLG72_00480"
     CDS             22918..23151
                     /locus_tag="PLG72_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOJ79311.1"
                     /translation="MNRTPLDRLALVLLIIGALNWLLVGVADYDLVTGIFGGNLFTGD
                     MSLFSRIIFALVGVAGLYSISLLFRESPAAERE"
     gene            23256..23447
                     /locus_tag="PLG72_00485"
     CDS             23256..23447
                     /locus_tag="PLG72_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOJ79312.1"
                     /translation="MANSRSKTAFENMKYEIASQVGVNLKQGYNGDLASRDAGKIGGN
                     IVKKVFEAYTGNTYQIEKK"
     gene            23684..23962
                     /locus_tag="PLG72_00490"
     CDS             23684..23962
                     /locus_tag="PLG72_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79313.1"
                     /translation="MRKLLLPVVVFFVILFFLYLPDLFPSCACCGKKKPRPFFRIHKA
                     VSLSPGYKGCRSVCTKCCRKHDLKDLKDLDRLIRVNRKVRISRLSDDF"
     gene            complement(23981..24307)
                     /locus_tag="PLG72_00495"
     CDS             complement(23981..24307)
                     /locus_tag="PLG72_00495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ79314.1"
                     /translation="MKKSTERFSDRVENYVKYRPGYPKEFIDYLYANAGFSEESVIAD
                     IGSGTGILTALLLERGSRAIGIEPNREMRNAEPLMAELEMLFDKYAADGKVSFEYETE
                     AFIGEI"
     gene            complement(24469..25206)
                     /locus_tag="PLG72_00500"
     CDS             complement(24469..25206)
                     /locus_tag="PLG72_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ79315.1"
                     /translation="MSGHSKWVNIRVRKGKMDAQKGKVFTKLGREIAIAVKHGGPNPD
                     SNSRLKDVIAKCKAANMPNDNIMRSIKKAAGEGDGANYEEIVYEGYGPGGVAVIVEAA
                     TDNRNRTAGDIRHYFDKFGGNLGQTGSVSFMFNKKGVIVIEKSDKVSEDELMMEALEA
                     GAEDMTAGDEYYEIITAPGDFSAVREALESRGYEFMEAEVSMIPTTTTRLTDPKQIEQ
                     MQKLIDSLEDMDDVQNVYHNWEQDDEE"
     gene            25384..26310
                     /locus_tag="PLG72_00505"
     CDS             25384..26310
                     /locus_tag="PLG72_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ79316.1"
                     /translation="MVLLRIALAAVVVLAAALAFVSLCLIKSNNNAKLLKKQFDSAIE
                     ANSRRTGFFSSMVHELKMPLSIILGAAQLIEMKRKSERQDENGMEKHIAAIKCNCYRM
                     MRLTGNLLDLARSEAGYLVLKPVNCDLCRLLEEIVRSVRPYAMKKQIGLVFRSAHDSI
                     VAAVDIEKMERIMLNLLSNAIKFTDPGGSVIVSAYSADGRVFISVKDTGVGIPPDRQN
                     DIFGLYSQAGIHSAEKEGSGIGLYLVKAFAELHHGNVRLISEEGKGSEFIVDLPSGTV
                     TSSPEVSDAGEFGIKAGDAVSIEFSGGHSVLS"
     gene            complement(26456..27088)
                     /locus_tag="PLG72_00510"
     CDS             complement(26456..27088)
                     /locus_tag="PLG72_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOJ79317.1"
                     /translation="MRKDYLTVSKEAAAEIEEKKSRFIATVKPVSSEQEAQDFINGLK
                     ARYWDAAHNVYAYYICADNVVQKFSDDGEPSGTAGLPVLEAIKKTGVQDVAVVVTRYF
                     GGTLLGASGLVRAYGKSAALGIEAAGIVRKMLCVEARVTTDYSLFGKLQAAMASKGYS
                     VKDTIYTDNVAMEVYVPVDESDRFSAMVTEASNGRARISAGGRVYITKEV"
     gene            complement(27353..27778)
                     /locus_tag="PLG72_00515"
     CDS             complement(27353..27778)
                     /locus_tag="PLG72_00515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ79318.1"
                     /translation="MIVRNCAGGIVFCGNKVFLLRNEKGEWVFPKGVVRNGELPNEVA
                     LARVKAEGGITAEIISTAGHTNYEFYSVTRQKPVCNKIIWYIMRTDSLQYEINEHEKF
                     VDGGYFDIDEAMKLVTYSQDKALLNLSYRKYRDIASVAV"
     gene            complement(28075..>29261)
                     /locus_tag="PLG72_00520"
     CDS             complement(28075..>29261)
                     /locus_tag="PLG72_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOJ79319.1"
                     /translation="FLLDGIRRLNGKGVYCWIVPNKLLVSQYAMPVLEYLKENGLRYT
                     ISVSDLNVFSGVGVYPVIVTGNRCEAGKKEIIGTRTRNDAGSCRASVHSDSCCIYTNC
                     SDPGKTGCTGVGSTDEFGVCGTDRYSSHDGLLEYAADSLQQLETRRFFSRSKTRQYET
                     FADHNIKIASGAAGFQAAVLKECIIEAQRAADATLREEHPMSDGCGNAIPFAVSGSID
                     RYCLDRSKVRYMGTIYENPYILKGEKIADSKWKLWCSEKICIAGMTKELEAYYSREPL
                     AIGVGTYAIYGFGGTDPFYLLALLNSRFMSWYLREKFYERHLAGGYIAVNKFMLEQLP
                     LVRTDKKTEQELARRAEKLQQNFCGGAMAEKLLSEIDEMVCMIYGLDGKETDRIRNAA
                     LG"
     assembly_gap    29262..29361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29362..30479)
                     /locus_tag="PLG72_00525"
     CDS             complement(<29362..30479)
                     /locus_tag="PLG72_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79320.1"
                     /translation="MGFDWGSRYREAFGSIYEKIGSVHDDSGRTACISGNSARFHNVS
                     SDRIPNPDVLAKLYTDTLQISHKKGSGVFYTPPEVVRFMCRESLAHYLSNSTGLSFDL
                     TLRFMERITADVQNNTDVDRELLLGADRALETIRVFDPAVGCGAFICGMLDEIVRLRS
                     GITWYIRGSSAGYTGDRSGRFIGDGSAQSGCSGRTSPDTAACCRPIPDRRPFMAEDRH
                     PFVLKYNAVANSIHGADIDATAVEIARLRLWYELITEYERYRSEAEEPGYAKSYAETM
                     NSATHDAGATCNTIEENGFKCNVSCADSLFEYDVCSFDVIIGNPPYVSAVEGSRSGRG
                     MRRALKKKFPQLKGSFDIYAAFLLDGIRRLNGKGVYCW"
     gene            complement(30498..>31139)
                     /locus_tag="PLG72_00530"
     CDS             complement(30498..>31139)
                     /locus_tag="PLG72_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOJ79321.1"
                     /translation="RGGERMENKVRVYIKVWGEGKIPQYASNHAAGCDLFATMDMAIR
                     PGETRIMPLDFSMAIDTGLEAQIRPRSGLSVRTELRIPNSPGTIDSDYRDTVGIIIQN
                     TYNIANLPYQVAVNPELLDELRENYEEVDLGTYLGRHCGPVVTNAAPLAVLGQKIYID
                     RQGNPYGTIYIKKGERIAQMVFCEHKRAEFVPHPDPRSIGEDRGGGFGHTGSR"
BASE COUNT         7332 a         8326 c         7376 g         7995 t
ORIGIN      
        1 tgataagggg tatatgacgt gtaaaactcg gagcgcgata tcacgtgcca gaccactgag
       61 ggaataaaat ggtcatagac gcctgccccg agaaagcacg gatggtcagc ggcattgata
      121 ttttttgaag cgacggctct catatgagaa accagttcag gctccgacat cgcggccggc
      181 agttccagct ccctgtccag cctgatgtct tccgggatat cggagaacag ttcgtcgata
      241 ctgcccgcgc ctatggcaga cagcatcttc tcctggtcct cccgggtatt cgggatgtac
      301 cggtacatat tactcctcct cggaacagaa ttcactgtac tcctcttcat ccatcagttc
      361 atcaagttct gacagatcgt cagggtctat gaccgcaatg taattctcgt acggatcctc
      421 gttgagaagc tcaggagagt cttccagcgc ctcgttcgcc tcaacgacag tgcctgaaac
      481 aggcgagtag atatccgatg cggtcttgac ggattccacg acactgatcg gatcccctgc
      541 cttcaccttt ttcccgatcc cgggcagttc aacatatacg atatcaccca gggctttctg
      601 ggcgtagtcc gaaatgccta tatatacctt gcctccttct tctcttgccc attcatgctc
      661 ctttgtgaat tttacccctt catacatttc catagcgctc ctcctcaaaa ttatattttg
      721 attaaaccat ggctgtcgta cataaacggt tatcgcttat aaaacggaag tttgacagtc
      781 ctggccgcat attttttgtc ccggaccatc acttccagat cagtgtcatc gtccgcgcag
      841 gacgtttcga ccagcgcgat gcccatattc tttttcagcg tggggaagaa cccgccgctg
      901 gtcacatggc cgactttccg cccgctgcgg tacacttcat agcccgtcct cggtattgcc
      961 ctgtcgatca tttcgaatcc ggcgagcctt tgcgccaaat taccctccat ctggctgacc
     1021 agcgcttccc tgccgatgaa gtcccccttg ccgggtttga cgaacctgcc gagccctgcc
     1081 atgaccggcg atatggtctc ggaaagctcc tgtccgtaca ggggcaggcc ggcttccagg
     1141 cggagcgtgt cccgtgcccc gagtccggca ggcatcagcc cgtatgcggc tcccgcatca
     1201 cagagcatat cccagagttt cagggcgtca tcaggcctgc agtaaagttc aaagccgtcc
     1261 tcgccggtgt acccggtacg ggacagcatc acttttacat ctcctatttg catttcattt
     1321 acataccggt agaacttcag atctgacagt atctcccggg catctccggc agtcgtcagg
     1381 ctacgcagga tattttccga ttccggtccc tgcagagcta cctgggcgta ttcatcagaa
     1441 cggttcttta tatcgacatt gcctttctta ttatcccgga gccattgaaa atccttgtct
     1501 gcgttggatg cgttcactac cagcaaatac gagtcgccgt tttgcctgta gacaataagg
     1561 tcgtcgacag tgccgccgtc agcatagcac ataggcgaat atacggcttt cccgggcttt
     1621 gcagtgatgt cgtttgtcag aatgtgctgg aggaactcaa aagcatcgct gccgcttatc
     1681 tctatctcgc ccatatgcga tacgtcgaac agtccggctt ttgtcctcac attttcatgt
     1741 tccttaagta tactgctgta ttgcacgggc agcagccagc cgtggaaatc gatcattttc
     1801 cctccggctt tcacatgcgc ctcatatagc ggcgtctttc tgatcgtgtt ttccattgta
     1861 tatatctcct atcagtttat actctatcat aaatatgcgc cggacacatc cgctgcaatc
     1921 ggcaggcagt gcccgttttg cgtcatatag ccgcatctgt ccgaaatatt gccctgtccc
     1981 ggatattata catgtatcgg aagcaggcta taaaaaccaa atgccgtcat ctcagaaaca
     2041 tttgcttctc agccagcctg ccaggtcata cgccattatc ctgacgtcat caggcccttc
     2101 catggccgct tccacgatct ggtccgccag ttcttccgcg ttgaagcgca tgcctttcat
     2161 gacttccgcc aggctttcga tatatgccgc atccatcgcg tcagaataca ctacggcctt
     2221 gcttaccatg gcgttctgga gcgtaaagcc gatctccaca ccgccccagg gaaacctggt
     2281 ctcataggtg acgtcaaagt ccggggcctc gccgtaccgc cacgcccatg acgaatactt
     2341 gcaatatagc tcctcgatgg cttccctgct ttccctgtcc ttcctgagtg ttccttcgac
     2401 gtccgccacg cttccgtact cccttctgaa cgattcgata agcgcttcgg ccgcgttgtc
     2461 ggccgagagg cccggcttca gttcagacag gttgaccacc cttgacttca cggattttac
     2521 gccctttgat ttcatttttt cctctgagac ctgaagatat ttggacatgc tgtccaggtc
     2581 ggtcgagaca aggaccgtcc catgcatata tgcgctttgc ttcctgatac agaaagcgtt
     2641 gccggagaat ttcctcccgc cggcggtcag gtcgttcctg ccgctttttt cggcatctat
     2701 gccgaggctt tttattgcag aaagtattac tccgaaccat cgttcagtgt cgaacagctc
     2761 cctgcccgac acgaacgtgt aattgaggtt tcctgaatcg tggtataccg cgcctccccc
     2821 ggaaagcctc cttgccagct tcccgccttc gctttccagc aggtcggtcc tgcattccct
     2881 ccatgggttt tggtttcttc ctatgaccac ggtgttttca ttttgccata tgtatagtat
     2941 gcactggtcc ggttccaccc gttcaagcag gtattcttcc accgcaaggt tccaccatgg
     3001 gtcttttgaa catgtccgta ttatgcgtgt atcatatgcc atctatagcc ccccaggctg
     3061 taacaggtat tatggttttg ttcccgtgca gctttaaccg gctgcggtac ttcggtcagt
     3121 tccatctgaa attatacttg acatgcaaaa ggaattcaat tcatctgtat acattcgtcc
     3181 gcatgtaaca taaacagttc agtcccaata ttatgtaaaa ggaatactaa tttaaaggag
     3241 tgcattctat gaggtaccag gaagatttca caggcaccaa gacagaattt ggcgatttca
     3301 tcaaaaaggc catcccggaa ctgttcgcag gaaggctgac agtcgaaggg aagacaatct
     3361 ccataccgtc ggacgtcacg ctggactaca aggtcaagta cgatgaggac gcagaaggcg
     3421 gatcagtatc gatcaaggta tcctgggata atccgaatct cgatcttgaa atcgaagaag
     3481 aggaagaaga agaataagcg ccttgactga tcagggggag ccgatccccc tgatgcagtt
     3541 ttgacgcagt tttggagtgc cccttggcaa tggtttgata cagtttctat acaagtaagg
     3601 aggttgcagc catgccgccg gaagtgcgtg aaccgctgga agaggtttta aagcattatc
     3661 ctctgacggt tttggatata cgcaatgagt catacaagga aaagaagggt gtttggtggg
     3721 tcaggacttc atcggggatg aagatactga aaaagatatc caactcggaa cagaccctca
     3781 gattcacgct cgatgcggtc aggcacctga ggaataacgg ggtgctgctg ccggaagtga
     3841 acaggacttc agacggaaat gaatatgtaa agatcgaggg cacatgcttc gtcctcacgg
     3901 atgccgtgga aggaaggaat ccgtcgtact cgtcgccgga agaaatggcg ctgatcgctt
     3961 ccgggctcgc ggatttccac cgggcatcgg aaggcttcag gccgtctgaa gggtcaaagc
     4021 cgaaatacca tcttgggaca tggcccgaag tctattctga aggacttgaa gatatgagat
     4081 cctactacga cagcctgctg atgaaaaaca gccacgatac aatcgaatcg atcatcgtca
     4141 gggaattccc ctatttttac gagcgcggca aaaaagctgt cgaagggctg aaagggaacg
     4201 attacatgag ctggagccag gaagccgaaa gcaagggctc actgtgtcac caggatttcg
     4261 cagcgggcaa tcttctgctg accgacagga aaaagctgta cgtgatcgac acggattcaa
     4321 tcaccattga catagcagca agggatataa ggaagctgct gaacaaggtc atgaagaaat
     4381 ccggcaaatg ggacccggaa ctggcctccg gcataatccg ccactaccag gccaggaacc
     4441 cgctgacacc gtcccagtgg gaagtggtga ggctggacct gcttttcccc cacctgttcc
     4501 tgggagcggt caacaagtat ttctacaagc gcgacaaaga gtggtcgtat gagaagtata
     4561 tgaagcggat cagggagatg gcggcatttg aacggacagc ggaacccgtg ctggagaatt
     4621 tcagccggat agtgccggtc tgagctaaaa gtttcattta catgaaccgg aggtaacaaa
     4681 atggatattg tgaacaaggc tgaagccaca ggcaatcttt caattgcatg tgatccagcg
     4741 attgcattca acgttctgaa ggaatacgga atagtcccgg acagcgtctc tgtcgtccag
     4801 ggcgggacgg tcaaaacggt atggaaagtg tcgtcaggaa gcacgctgta ctgcctcaag
     4861 cggctcaaac atacgatcga caaggccctg ttctccgtca atgcccagat ccatataaaa
     4921 aactccggcg gacttgttcc agggatcatc ccgaacaggc gcggagagcc gataacacaa
     4981 tacaacgggc agttgttcgt cctgtatgag tggatcgccg ggaaagacct ggatttcaat
     5041 gttcctgccg atctacggac ggctgtgcgc gggcttgccc gtttccacct tgcctcaaag
     5101 ggctatcagg ccgccgaaag cgcaagggtt tccacaaagc tcggcaaatg gcccgaacag
     5161 tacatgtcta tgagcaaaaa gctttcctcg tggaaggagc tggcggaacg cagcagcccc
     5221 agccaggcgt tttcctcata tctgcagcat gtcggcagga tgctcgagct gacggacaaa
     5281 gctttgaaag cgctgaacgc atctgattat acaaggctgg ccgggcaggg atccgaccgg
     5341 gcagtgctct gccaccagga ttacggcaaa gggaatgccc ttgcggtggg aagcagcgtc
     5401 tatatcctcg acctggacgg agtgacctgg gacctccccg ccagggattt aagaaagatc
     5461 atcggcaaac acgccgaaag acacaaccgg tggtctgccg aagccatttc ggagatactc
     5521 ggctggtaca cggaaataaa cccgatgggg gcggaagaga tggaagtttt atatgccgat
     5581 ctcctgttcc cgcactggtt ctacggcctc gtcaagaacc tgttccagaa cggcaaaaca
     5641 ctcaaggcat ctgaaataga aaaaactgcg ctgctggagt cgtccaagga agaggctttg
     5701 cggctttgct tcaaaaggag tgattgactt gaagatagcc ctcatctgta ctgaaaagct
     5761 cccggtaccg ccagtggcgg gcggtgcgat ccagatgtat atagaaggta tccttccgta
     5821 cctgtcgccg catcacgata taacggtttt ttgcatccag caccccggcc tgcctgagga
     5881 agaagtcagg gaaaacgtac ggtatataag agtgcccgga aaatcgcaaa ccgcttatat
     5941 acagaatatt gctgagagca tcgcggcatc cgtcgataag gacggaacgc cgttcgaact
     6001 gatccacgtc tttaacaggc cgaaattctt cctggcactg tcggaacggt tccccgggtc
     6061 caggttcggc ctcagcctgc ataacgagat gatgcatgct gagaagatct ccgacgatgc
     6121 cggcctcaaa tgtatcgaaa aagcagagtt catcaatgcc gtgagcagat acatcgcagg
     6181 cacggtcacc agtcgcttcc ctgctgctga gacgaaaatg cgcgtggtgt attccggagt
     6241 cgacccgaaa aagtatgatc cgccgtggac tgaagcaggg gcggcgaaga aacttgaaat
     6301 aaagtcgaaa tatggcctgc aggactacag ggtcgtcctg tacgtgggca ggctgagtat
     6361 aaaaaaggga gtccacatat tgatcaatgc aatggaaaaa gtgatgcaga cccacccgga
     6421 caccgcgctg gtcatcgcgg gcagcaaatg gtacggcagc aacgcaggcg atgaatatac
     6481 cgactctatc cgcaagctcg tactgaaact tcccgggccg gtcgtgttca cggggttcat
     6541 acctccgtcg gatatcgccg gctattactg cctgggagac atattcgtct gtgcctccca
     6601 gtggaacgaa ccgctggcaa gggtccatta cgaagctatg gcagccggca tccccataat
     6661 cacgaccgac cgcggcggca acgcagaggt catgtatgga acggatgctg ctgcagcagc
     6721 gaatgccgca aatccggcag gcacagcagg aggctgcggt atcgtgatag accaatacaa
     6781 tgatccggca gtatttgccg acaaaatacg attcctgctg gacaatcccg gtgttgcttc
     6841 ttccatgggg cggaaaggca ggcagttggc agaggagaag tataactgga aaagagtggc
     6901 cagtgaaata ttcccgttca cggggcatga catttcacgc gccgatatgc aacaggtcaa
     6961 tgtacatccg gatacaggga cgggcatgtt ccgcgaaggc caggtccagg tcgctgcaag
     7021 aaaaccatca gccgataatc ctgctgttac tgcaaatata tcatcatatg ccgcaacact
     7081 tgcagatgaa agtatacaga ccgcggccgc aataccggcc gccattacgc atattgcagc
     7141 cggctcgccg ctatccggta ccgctacggc tgccgatacc attgcacccg gcgccaatgc
     7201 ccataccgcc aatatatctg tgatcggaac aggatacgtc ggcctggtga ccggcgcatg
     7261 cctcgcatcc atgggcagga aagtgatttg ctgcgatcag gacaggaaga aaatcaagga
     7321 tctcagcagc gggatcgttc ctatatatga accgtcattg aaagagcttg tcgacagcgg
     7381 tatcagggaa aagagactga gctttacaga tgatatccgg caggctgtcg gacattccga
     7441 tatcattttc atcaccgtca acacgccgac ccttcccgac ggttcctgcg atacaggcaa
     7501 tgtattcgaa gcggcgcgca gtatcggtac ctgcatggac agatacaaaa tgatcgtcaa
     7561 taaaagcaca gttccggtgg gtacgggcaa aagcgtcaag caggtgatat cggcaatgct
     7621 ttccatacgg ggcggcgctt gaatactgca gcgacatata ctcggcctgc tccgggagcg
     7681 attgtatcgt actggctacc gaatggaagg agttttccga tcttgacttc ggaaagctta
     7741 aaccaatagt gagaaggccg gtattcatag atctccggaa tgtatatgac cctaaaaacg
     7801 ttaagagttc aggtttccat tacgaggggg tgggaaggaa atgaaagtac tggtcaccgg
     7861 cggagccggc ttcataggtt cccacctgtg tgaacgcctg cttcgcagcg gatctgaagt
     7921 aatatgcctg gacaatttca gtgacagcta taccccgtcg gtaaagagaa aaaacctcga
     7981 ttgctgcgtg cgctgctccc atttcaggct tgtcaaagga gatatactcg atcccgaagt
     8041 aacggatgcc gtcatggaac aggaagcgcc ggaaacaata gtccacctcg ccgctcttgc
     8101 aggggtcaga ggctcggtgc tcgacccgct cagctatgta gatgccgatg taaaaggcac
     8161 tgttgcaatg ctggaatgct gcagaaaatt tggtgtgaaa aaattcattt ttgcatcatc
     8221 ctcgtcggtt tacggcaatt caggcacgcc tttcatggaa gaaaacactc cgccggtgca
     8281 ggtttccccg tatgctgccg cgaaatactc cggggaactg ttttgcagga catacagcca
     8341 tctttatggc ctgccggtgg tatgcctcag gttctttacc gtatatggcc cgagacagcg
     8401 gcctgacatg gcggtccata ctttcgcgag ggcaatcgtc gaaggcagcg aaatcaaaat
     8461 attcggcgac ggcagcagta caagggacta cacctatgta gacgatgtga tcgacggcat
     8521 aatgtcatcc atagacctgc cgtgcagtta tgaaactata aacctgggaa actcggttac
     8581 cacaagcatc cttgaactgg taggcatatt ggagagcaag ctgggaaaaa aagcaaaact
     8641 cagtttcctg cccgcccagc cgggcgacgt gcctgcaaca tgcgcggaca taacaaaagc
     8701 aagggcattg ctgggctatg acccgaaagt gccgctcgag gaaggcatcg aacgctttgt
     8761 tgaatggtat ctcaaaaggg ttgccggata acggcgcctg gcacatcagt cggactgttg
     8821 cctgaagaaa ataaaacacc atgcgcgata cttagacagg cagccagaca gggagggcgg
     8881 tccccgccag acatagggac ggttcccgta tctcagcccc aggtccggga tacgggaacc
     8941 gtccccatgt cttaagtatc gtgccaggca tcacttcatt acttcttcat ttctttggat
     9001 atctttctcc tgaagtcatc ccattcaccg gtctttaatg acctggtcag gtttttttct
     9061 tcgtgtacat aagcagggac ataacctctg gtgaccctgt ccagacacct ttttgccatg
     9121 ccttcttccc atacgatctt ctttgtgatc atatatccac atcctaacat aacaatatga
     9181 tttacataat tattatatta tgatgcggat aaaaggttac ccttattgtt gctttgtcat
     9241 tttccaacaa ttgctctgcc gcttccgtct gtgcatattt tataagtcct gccgctgtca
     9301 tcataatttc tgtaataaat ccaatctccc actacgttaa tatcgccgct gtcatcatca
     9361 ttaacttttg tccggacgct cccgtctgtg cggattttat aaatcttgcc gccatcgtag
     9421 taatcatagt aataaatcca atctccctgc atagctgcaa gtccattgtt cgctaaattc
     9481 ccaaccgtgt ttcccctttc aattgatgca tttgccggag ttggtccagg ggtctcggct
     9541 tgtgcgggtg ccgttatatc tgtttgcgca ggcgcaagta tctcttccgg ctcagacgcc
     9601 ggctccgggg attccgtcag ttccggcgcc ggttctgaga ccgctctgct ttctttttga
     9661 gaagccggcc ttaattcatc aagtttttcc atgattctct tgctgtttgt cttctgatac
     9721 gcttcttcca acagaaggat cgcgccatcc agatcccggg cctttatttt cagatccgcc
     9781 aattttatgt aaacattatt gtctatattt tccgcttctg cttttaatat ctcttccagc
     9841 agactgatgg ctttacctgt ttcacccttc ttttcgtaaa cctcggccag cctgatgcct
     9901 gcctctgcca tgccaggctc cagttccagg acttctttca ggcatatttc cgcattcccg
     9961 ggatctcccg tcataatgaa agcttccgcc agcgccatcc tggctccgat attctcaggg
    10021 tcgatcctga ttgctttctc aaaagcccgg atcgcctctt catacttccc ttccagcaaa
    10081 tgtccgttcc caagctcgag atgtttcgcc gcattgttca cgcctgtcag cgaaactgcg
    10141 aggacgctgc cgccagccag caggattatc gctgccgcag caatagtgaa tctttttccg
    10201 gacatgtatc acacctcttt tatatattgc aaaatgtcct ggcaaagctg atcgccgttg
    10261 atatccggtt cagcctcatt tgttatacgg taggcaccag taaaaagttc cattcgtttt
    10321 attggattat atctatccgg gatacaggca ggaaaatctc tgccgttcat tgaccgcaga
    10381 acgggcgata agccggatga agaaatgtgg cttgagttgt gcgtatgtgc ggataaataa
    10441 aataataaag gccggtttag ttggataata ccggcctatg gtaatacatt ttagcaaatt
    10501 atattcgata tacttaatat atagtcctgg cacagcggga cggctatttt ttatatgacc
    10561 acctgactcc ctgcggagtg tcctccagca atatgcccat ccggttcagc ctgtccctta
    10621 tctcgtcggc gaccttccag tttttctctt tcctggcctg ctgcctctgt tcgatgagcg
    10681 ccattatctc aggatcgagt tcgggtgttt cttcctttgc cttcaccttt tcagcagcct
    10741 tgtccaggtc gagagcaaaa acctggtccc atctgagcag cagcctgtag agatcctgtg
    10801 atttcgtgcc gtaccgtacg acattccagg cgacagccat ggctttgggt atgttcaggt
    10861 cgtcgttgat cgagtcctgg aattcattct cgaatccctg tatgatttca tcaggtattt
    10921 ttacttccgg atcggcattt ttatgggcaa ggactccctc aagcagcctt tccagcgccg
    10981 tctgcgcggc atccagggca tcccatgtga aattgagctt gctcctgtaa ttcgcgttga
    11041 ggcacatata cctgaatgcc agggggtcgt atcccttctt tttgagatcg tcaacagtgt
    11101 atgtgttgcc aaggctcttc gacatcttcc cgttgtccac cagcaggaac tcgccgtgca
    11161 gccagtaccg caccgcaggc ttgccgagca gagcctccgt ctgcgctatc tcgttctcat
    11221 ggtgcacagg aatgtggtcc acaccccctg tgtgtatgtc gaattcgtcg ccgaggtatt
    11281 tcctcgccat tgcagagcat tcgatgtgcc atccgggata gcccatgccc cagcggctcg
    11341 gccactgcat gatatgctcc ttcggagctt tcttccagag cgcgaagtcg gccggatgcc
    11401 ttttttccgt gttcacgtcg accctggctc ccgctttctg gccttccagg tcaagccttg
    11461 acagctttcc ataacccttg aatttcatta tgtcaaaata gatgccgtcg ctcgtttcat
    11521 aaccgtaccc cttttcaacg agcccttcga cgaactcaag catctccttt atgtgctcgg
    11581 tcgcttttgg gattatttcg ggcttgtcta tattgagttc cgcaatatgt tccataaaaa
    11641 cttttgtgta atattctgct atctcccagg gagatttctg cgtttccctg gccgttttga
    11701 ccattttgtc ctcgccttcg tccgcatccg acaccaggtg cccgacgtcg gttatattca
    11761 tgacatgctt cagcttgtag ccattgtatt tcaatacacg cctgaggata tccatgaaaa
    11821 tgaacgaacg catgttgccg atgtgggaat actggtagac ggtgggtccg catgaataga
    11881 tctttaccgt gtttccctcg atcggcctga actcttcttt ttgcctggta agcgtgttgt
    11941 agagcttcat tttatcttct cctcccgttg attgtccgat gagtggcttc gccgtatagt
    12001 ccgcacctgt tacagacagc acctttcatc aggtatcatg cgcatatgtg tcagcgtccc
    12061 gttcctttgc ttcaggcatc ctgcggcttt gtgccggtgt ctgccgcatc ggcgccacta
    12121 tcctgcttcc ctgagtcaga atcccgggac tctgtgccgc tgtcctgtga cctgttgtca
    12181 gcgtcctgcc ccctgagcct ttccctgcat gcctggcggt cgatcgctgc cgcagaccgg
    12241 ccttccgctg ccgcttccag catttctatc ctctcgatga tcttgcaaag ctcctgcgcg
    12301 accgggtcgg gtatattgat ctggtccagt tcaatggacg gcgccagttt cctgccatcc
    12361 tgcctgacga cacgcgcctt gactcctgca acggtggaat ttggcggaac gtcgttgaga
    12421 acgacggagc cggaacctat catagcattg tccccgaccg tgatgggtcc caggagtttc
    12481 gctcctgccc cgatcagcac attgttccct acggtcgggt gccttttctt acccttttcc
    12541 ttacccgtac ctcccaatgt gacgccgtgg tatatggtac agttatcgcc gaccactgcc
    12601 gtctcgccga taacaatgcc cattccgtgg tctatgaaca aacctctgcc gatttcggct
    12661 cccgggtgta tttcaatacc ggtgacgcac ctggcaaact gggataccag cctggcaagg
    12721 aaaaacactt ttttcctgta cagccagtgg gcgatcctgt gataaatcag gacgtggaat
    12781 ccgggataca gcaaaacgac ctccagagtg ctttttgccg ccggatccct tttcgctata
    12841 gatcttgcat cactcaacaa tcccctgaac ataaaccttc cccgctatga tcaccaaaca
    12901 cgttccggcc tgtccttctt tgccggtgtt ccacaaggcc gggcaaatac tcctgtttga
    12961 tcctttacag aactatatgc acatcgcgtc aaaatcgtgc ccgcatgcag atcattttat
    13021 cagacttatt actgctatat ccgcatattt cccctgcatg ctgtatttat tatacccaca
    13081 tgccgtacct ttccaacatt atattcgcct gttttctcct atgcaaaaac tccgcttccg
    13141 gacgagaagc gaagttgtgt catggttttt tcattttatc aaaatttagt ctaaaagtaa
    13201 acaaataaga tgatccgacc ggtataaaca tgtgaacgta aatgaataat ataactgacg
    13261 gcggtttgca ttttcatata tcctttgaaa ggaagtgata tgattggctg aaaatgttga
    13321 aagatcgcaa cgcttcagta tcgggcagtt gcttgtaaga tggctggtcg gcgcaatagt
    13381 gcttgccgtt acagcattcc ttacgccggg tttcacgata tcgggcatat ggcctttgat
    13441 cattgccgct gcagtgatag cgatacttga ttatatcgtg gagatgatcc ttggtgtgga
    13501 tgccacacct ttcggcaggg gactgaccgg ctttattgtt tccgctgtga taatctacgt
    13561 cgtcaagttc ttcgtcgcag gctacaatgt taccgtactt ggcgcaatac tcggcgctgt
    13621 cgtgtatggg ataatcaacc tgctgatacc ggggagaacc atgtaactta atatgggagc
    13681 agatccaacc gacatcatat agaaaactgc tgctcaaaac atgcgctcat accggccgca
    13741 tgcgtctatc cgactgaggg ggcttccgcc ccctcgatca tgtatcgggg agggatatcg
    13801 gccgtgtgcc gtccatggat cacatccggc aaatgtgcag caataaaggg atggcactgc
    13861 caccccttta tgtaggaccg cttcactctt cttccgcaac agacctggtg tcctcttcag
    13921 ccccggggaa atctgctttt tgaggcggga agaactcgtc tatcgggaat tcgattgtgt
    13981 cgaacaatcc gcacgcgtct tcatctgttg ccgctacgca ctgtttgtta ggaacgcaga
    14041 agtcaaatgc gggtatgaga agctggacat atctcgcgag cttgacgatc gagaagaggc
    14101 cgatggtcgc aacgacccgt ttgcctatcg taagatgcct ttcctctcct ctcagcagat
    14161 cggtgtcgtc aaagtcgacg tcttcaagaa ggtcgctgac agttgcgggt atgcccctgt
    14221 cgttcccgtc atcacaggcg ttttcaagaa cctccacgat cttcgcattg agcgttatgg
    14281 gttcggccac ctctatcttc gagatgggga ggttatcctg ctgcagtgct gatgagccct
    14341 gatcatcaag gcccttgtca gcaaagtggg atttgaatat cttcacgttg ccttccgagc
    14401 cgaacaggat gactttcttg tcgaacatga ctatgccggg gaccgttatg acccttatgc
    14461 cgccgttgcg cctcggtacg aagaagtcca gcgtcacctt gatgaagaat ttgatgtcaa
    14521 ccgtgaagaa acctctcttg aaaggcactt cctccacatc ggagaaaacc ttcaccactt
    14581 cggcgcgcct ggccttgaca ttgatggcgt tgttgaccag ccagttcacc ttgtcggggt
    14641 tcttgaagaa aactctcgca tcttcgatac aatccttctc cttgcatgaa tcatatattt
    14701 tttctacctg tatacaaact gcctccctga tcttgcacag atcccgttcg ctgatcaggc
    14761 cgggaagcac tttatcatgt ttacccatat ttatatcccc cttcgcacaa aagtttgttt
    14821 tttcgatttc cctctcattt tcataatatg ttgatggtaa atatttgtga tagattacgc
    14881 ggcataatga atataattgc gctataaacc ctcatatata ttaaacagtg tagtttaatt
    14941 atgtgagtgg tcagacatgt tcaattttca taaccggcac ttcatcttca ggcatgcgga
    15001 cgggctcgta cgtgatttct actgtgacca caggcaaagc atttgctgca gcatccttgg
    15061 caggaacggc atatggagcg acgtcagcat tattgcaagg gacgcgcacc agtacttcta
    15121 tgcggagctt ggcagggaca atttttatca catactgtac caggacaaca gcgggaatat
    15181 caattattca aaaacggacg gacaatactc gaaaaccttc ccggtccttg ccagcaagac
    15241 gccggcggta tacaacaagc acctgtatat cgcgccgctg gatgacgaga tctacctgtt
    15301 ctatgtgctg cagcatgaaa actcgttcct tctcgcatac cagacagtta aaaataacag
    15361 gccgggtacg ccgaaaatag tggattacgt ttcggggaac agcataccct gcgcggtttt
    15421 gtacgactcc gaccagaaca tttatgcatt ctaccagtcc tatgacggaa aatacctgca
    15481 gttgggatac aagaaattca gcaccgtgcg gaagcactgg agcgatttca cggcagtgac
    15541 acggtaccag ggcaactgcg agtacccgca tgcgaccatg gacccgtccg gcacgatcca
    15601 cctgtgttac cagaggcgcg cgccgaagct gttcgagttg gtgtaccagc agaaagcgcc
    15661 ggacaaaaac ctctggtctg ccgagaccgt gctgcactcc tccgtccatc cgtttgagaa
    15721 cgcctcgatc cttcatgtta acggcaggat catcgtatac tgggtccgga acgacgtaat
    15781 atactggtgc gccggttccc cggccggcga cggctggacc aggccggcca ggtacggcta
    15841 ccagcccggg cacaggctcc agtgcgtctg cttcaaagac ctgctggccg gagacagtcc
    15901 cggacagggg gacggttctt cactttctct ttcccccggg atattcccgg gcgtagtctc
    15961 cgatggtttc aatctcgcgt tcatggatac cggggacatc accggcgggc agccgttcca
    16021 acagatcccg ccgccgtcag cggcagcaac cggagatact tcgggaagtg agcttaaatc
    16081 gctggtcctt agtattttca aagagatgca ggacaggata agcgagatga aaaccgagtg
    16141 ggaagaaatc aaaaaggaga tgccgggact tacaagcgca tatatggaac tggcaaagga
    16201 aaccgaaaag ctttcgatcc ggctcaacat gcttgaaaac aggctggcgc agataaacag
    16261 ccaataccga agaactgaac cttcgccagg tactgtaaaa gggtcggtac agccggctgc
    16321 ggacaggttt gagccaactt ctgcccaggg tgcgccaacg gctgaatcga agcatcctgc
    16381 atcagtttcc gaacaagcgc cggtttccgt tcacggatcc gcacaggctg tacagggtgg
    16441 tcctgtcacg acttctttta aagatgtgac gcataacggc atgaaaccct catccacagc
    16501 aaaagatcac cctgcggcgg atgataccgc cggcaccgtt ccgtcggaaa cagcgtccca
    16561 tgaagcacct gccgggcgcc cgaagatatc ccccgaaccc gaatggctga agatatggga
    16621 agagtggaag gaacctaagg aatggagcga aggaggttaa aaaaagagga ctgtctgtaa
    16681 agacaagtcc tctgacattt ttaataaacc cgaaaagccg tttccctata ttttgacacc
    16741 tagcccaaaa gctaggtggg tgtcctgttg aaagcttctg cctcccaccg ccgcacacgt
    16801 acgggacgtg tgacggaatt agcttgtgac ctgacatctg cttccaaacc ccggactccc
    16861 ttattatcgt tagcaggttc aaaattatag gttatccgga catttcaggc tgatttatat
    16921 tcgcttttca caggccggtg atcctgtctg catcgtcctg gcgccttgcc cgcgccgctt
    16981 ctgtgattac attatatcat cgatttcccg gctccaacaa cacacaggag ccgcaattac
    17041 gcctgtaatg cgctgtttag ttctctctgc ctctgttttt ctttattgtt cagatgattt
    17101 tggaaccatt aaaaggtatt taatggaaaa agcgcccccg actgcggata ccgcagccga
    17161 tcgggcgaca gatcgaaaaa tggccgacaa tcgcagcatg atcaatatat cttgacataa
    17221 ttgtttttca tcaaatgcgg ttcgatcttg tatatctctc ttattacnnn nnnnnnncta
    17281 tcttgttgaa aagcgcttcc tgaagcacga tatccttata ctcgaggggg aacttgaggc
    17341 aatacccgca gccgcccttg gcgatctggc aaggggtcgg gaccacctcg aacacatatc
    17401 cctttttctc caggatggag ctcagcttca gtgcataatt acccgaatcc agcgtggcaa
    17461 cggcgtacat ttgaacactc tcccttcatt atattttatt tggggagtgc ccgtcatgtg
    17521 cctcaacccc ggtttcgtca cttctatatg ccgattctgt gctccctggc ataatagaac
    17581 aggtactgct gggcaaagcc tgcatactgc ccgaattttt ctgcggcaaa ccggtgtatt
    17641 tcggaaaaac ttgcctcacg gccaaaataa agctcctcca tcacccgttt gacccagaca
    17701 tccacgggaa agacatcata cctggcgccg ctgtacagga gcacgcagtc ggcgactttg
    17761 ccgccgacac ccttgtactt cataagttct ttacgggcat cagccgtctg catctctgca
    17821 agcctgcctg tgtcgaaccc ggactctgcg accgcggcag ccgtttcatg tatataaccg
    17881 cacctgtagc ccgcccggca ttcggagatc tgctcaaggc tgcaatctgc aagggtctgt
    17941 gcgtcgggga aactgtaata tggctttccc gcatattcca gttcagtacc gaacagcctc
    18001 gatatctccg atatgatttt catgatcctg ggtatcctgt tgtttgctga taaaatgaac
    18061 gatatcaggg tttcccaggg ttcctgcttt aaaatgcgga tccctctgcc gaattccacg
    18121 gccttttcca tgatctcgtc cgtcgttacc gcttttttga tcaggccgta gtccctgcca
    18181 aggtcgaaat agtcgaacca gatctgtttg aagtcctccg ccgtcacatt gtccagttcc
    18241 aggacgccgg gacggtaaag cacattggcg acctttcccc tgacaacgcc ggtatagctg
    18301 ccgtcaggct ccctgtacca cctgaagcac tgtccgcatt cgaatatgtg gaccgggtcg
    18361 aaatcgcgta tcccctctac cctgacgccg tttccttctt catatatgtc atatcctttg
    18421 tagtgcatgg catcctccgc tgatgaacgt cgcgacatat tttactgaaa caattaatac
    18481 ccttcctgtt ccgtcgaaac acggccgacg aaaggaaatt ttgagcagcc gtagaatttt
    18541 ttcagcgatc atgtgttata atcatataaa gtccgactcg attgtgattg taccacaact
    18601 gattacttaa cagggagagg ggcagcatga aagaaaagat ctatacaata ccggtaaccg
    18661 aagcattcag cgaggattgc gaatgtccgc tgtgtctgct ggaaagcagg ctggagaaag
    18721 aatatatcga ctattatctt ggcccgtccc tgatggagcc ggactgccgg atcgatacga
    18781 ataaaaaggg attctgccgc agacattatg aactgctgta caacaggcag gaaaaccgcc
    18841 tggggcttgg cctgataacg gatacgcata tcagggagca gctggagagg cttaaggacg
    18901 ccgctgagcc actgcttggc gggcacggca gttccagggg gccggggttt tcgttcggat
    18961 cccttgcgtc taaattcggc ccgaaagccc aaaccgactc gggaccggcg tcgaagctcc
    19021 tgaaactgct gacggaactg gaaaacagct gtaccatatg cggccgtctc gaatatacaa
    19081 tggacaggta cctggatgtt gtcatgtacc tttgggctaa ggaaagcgac ttcaggaaga
    19141 ctttctcgga gaaaaaggga ttctgcctgg tacacttcag aatgctcatc caggcggcgg
    19201 agaagtatct gaacgccgac aaaagaaaag agttcctcag gatcctggcg cagcagcagc
    19261 ttgaacatat ggacaggatc gagaaggaac tggaatggtt cacaaagaag ttcgactacc
    19321 gctacaacga cgctccctgg ggcaactcca gggacgcttt gccgcgcagc atacagaagc
    19381 tcaagggtta ctgcagcctg aagtaaaact caccccacct ggatatatga gttcccatcc
    19441 agcgggatga gcacctgcag cggcacgctc ccgtccatcc tgccaaccgt atatatcgtg
    19501 tagaaacgtc cctccagcag ctgtatattc ggcacataca ggagagtagt gtcggtgttg
    19561 ctttgcttta tatagaaaat atacgttccg gcaggaacag acaagtaatt ggtgaccatt
    19621 ccgaatgata catcagtgaa caattcagta ccattcgaca aggtcaggtc catatcaggg
    19681 ccgtccggca ccaggttgcc gaaccgcaga tacaggcgcc ccggcggcac ctggaccact
    19741 gtttcgaaaa acggcctgat gctcaatgcc ggtgggacgc ccataagcgc caccgtattt
    19801 atcgatcttg caggtatctc gattacggca tccagcaaaa ccggcccggt agtccccgct
    19861 gcaaatactg tcatattgta gctccccggg accacctgaa agtattcggt aaagccctta
    19921 tacggcaagc tttgggctat caggctcctg ttggcataaa tgtcgacagg ctgcaagccc
    19981 ggcactgcat tcatgagcct tatatatgaa aattgctgtc tctggttttg aaaagacggt
    20041 ccctggtatt ggctccgctg cctcatgcac ggcatatccg ccggaacata tgcacagggc
    20101 ggaaacatcg gtctcatccc gccgctgaat cttccggcgg tatgaaataa actgccattt
    20161 tgcatccaaa tcaccttcca cggctgaata aaaacaccgg ggacgcagat ccccggtatc
    20221 attatatttt ccgacgccat taaaggttac gccgcggata aggttatgcc ggcaccagca
    20281 atatgtcccg gccagggaca tgtccgcacc cggcattccg cgcggcagcc tggcatgcgt
    20341 cctttcattg ccgactctcc cgcatccgtt tccgtattac tgtttttcga ccacaaggct
    20401 tttaactgtt acattgaatt gctggatatt cagggcagtg agccgctcga tctcttcgga
    20461 taccttctgt tggagttccc gtaccagggc gcggatcggg taaccatact taagtataag
    20521 gtccatttcc atgtagatgc cgtcaggttg gttctccgcc ctgaaacgcg atatcctgct
    20581 gactccgctg aagccgctga caacgaattc gacgatctgg taaatagtat aatcggatat
    20641 ggtatagctt ccaagataac tgaacgtagg ccttacaacg gatttctcgc cgtccggctg
    20701 gaaactcagc tttcccttgc ggcggaatat ctgcagcgga tcgaggaaat aacctgaaaa
    20761 gtccttcttt atctcaaagg cagggaccgg tatgacatgc ttgccctgtt ccctcctcgt
    20821 tgcgagcgcc tgctttatct cgaactcgtc ggcaacatcc tcaatgtgga tgcgttcact
    20881 gacttcaggc agcccaagcc tttcggcgat cttgtccacc atgccctcag acgtgccgag
    20941 gatcatgatg gcttccggct ggtgctgctt tattgctctt ttaacttcat cgacatggcc
    21001 gtcatcggtg aaaagggccc gcttgatgga acctaccttc gttttttcct ttttggccga
    21061 atagcctgca atgacccggt tttcttttat aaaaaggcca tcgtctatta tatactgtat
    21121 tcctctttcc cttgctaccc aggacgccct gtggctttta cccgtgccgc tggcacctgt
    21181 gaaaccgact acccgcaaac ggacgacctc ccgatgttgc tgatatacta tatattatca
    21241 gatggctcac aggctgacaa cgaaaaaaac ccgtataaaa gggaaaaaga gagtcgctga
    21301 ctctcttttc tttccccgga tcttatcatt tgtatgctcg ccgatttcat ctcttgtttc
    21361 tcttggacgt ttatcttttg taagaaaact gtataatata ataactatat tatgtacttg
    21421 ttccggtgaa tttatacatt atattgtgtt acacgtatat tttatcatct aaagcgagaa
    21481 tgtcaatata attaataaaa atttttgtaa aatttcgatt gtagaaataa ttccctactt
    21541 ctgcggtgtt tcaacgaggc gatgtttttt cgcctcgtta cattatatgt tttaaaaaat
    21601 ggttagttac tgcgtcaaat caacatcagg gggatgatgg agccgatgtc cgtcacgcat
    21661 tcatcagaaa gctgcacgac ggttttgttt ctttcagaca ggaacgagac catcgaattg
    21721 aagcccttca acttttcgat acagccgttg atcgcacata cactgccgga tatcatgccg
    21781 caggctccgc ctatgaaacc gtggttcagc ccgggaagcc tgatcgaatc cccgcattcc
    21841 aggtagagaa cgtcgatccc tttcttcccg gcggcccttt cgataccgga atcggtggtg
    21901 ataatggaat tttcgtccac gggaaggatc gagcattttg aataaccctg ctccacatgt
    21961 atgggttcga tgcctaaccg ttccatgaag gccttgattt gcgggtcggt gtatttcagg
    22021 ttgtggaagt accattttcc gaccctggcg acattatatg caatatcata cgggtatttc
    22081 gacatcaggg cgctttggcc cattaccatc cggaatccgt atgcgtcaag cttcgagaca
    22141 gcttctgcat cagtgccggg ggcgtagacg atcatatttt cccctatatg atgcagcatc
    22201 atgtccggat ggcacgaaac ggcctcataa agcccgggat gcgggcgaag cctgacaagc
    22261 tcgataccga gcgcacgcaa tgcattttca gctttccggc ttatcctgcc gtcaacgaca
    22321 gccagcccca ctttgccttc cggcagattc ggttcctgta tgaatttcat ggcacgctcc
    22381 gcctggacct aataattagc atcgattatt tacatcatat tatcacaaat cagtctttat
    22441 atacataata tacaaggtaa cacttgttcg ctgccaacct tcatctgcag cacgacaaca
    22501 gagcgttact aaccgtcgcc tgccgcggct ggcgccggtt attcaaataa aaggccggcg
    22561 gctgaatgcc ccggtccttt tgttttctaa tgaatttttc aatgcttttt tgaaataata
    22621 atccggaaaa cgatgcaaaa taaacgtatc aaagcatatg tccaaaagcc accaaatcta
    22681 ccggggagga actgccatga cgactatcaa ctgttccttg aactgcaaac atcagaaaga
    22741 cggcagttgc atgcttgaag atgccgcatc cgacaccctg tcagtggata cggactgcgt
    22801 cttttttgaa gaaaagcatg acaaggccgg cagttcagcg tcttcacggt cttctgaatg
    22861 ataatggctt catcgggaat aatatcgaac gtaataataa gcaaaggaga tgtttatatg
    22921 aacaggacac ctttggacag gctcgcgctc gtgcttttga tcatcggcgc gctgaactgg
    22981 cttctcgttg gcgttgcaga ttatgatctg gtgaccggga tttttggagg aaatctgttt
    23041 accggggata tgtccctttt cagcaggata attttcgctc tggtcggtgt cgcaggcctg
    23101 tattcgattt ccctgctgtt cagggaaagt cccgccgctg aaagggaata gcagccgaac
    23161 aattatttac cgtcataaat cctctctgca atacaaatca taataatgcg ctgcaaaaca
    23221 aaaacggcgg cgaaaataaa cgaggaggga actatatggc aaacagcaga agcaaaacgg
    23281 cttttgagaa catgaagtac gaaatagcca gccaggttgg cgtaaacctg aaacagggct
    23341 ataatggcga cctcgcttcg agagacgccg gaaaaatagg cggcaatatt gtcaagaagg
    23401 tttttgaggc ttacaccggg aacacctatc agattgagaa aaagtaagga cgactggaag
    23461 caggaactga aatagcagga taaaggatca aaaacacaag ggctgtcgat tatcggcagc
    23521 ccttgtgcta tatgcggcac agtactgtaa tatgctgtac attctgtttg cctcatcgat
    23581 tcttttagct ccgggcattt ttcaaatact tttcaaaacg cacctgcagt atcccggcat
    23641 aatacacgca gtttgaaaat aactttattg ggggaggttt tgtatgcgga aactgctgct
    23701 gccggtagtc gtcttcttcg tgatcctttt tttcttatat ctgcctgatt tgttcccatc
    23761 ctgcgcatgc tgcggcaaaa agaagccgag gccctttttc aggatccaca aggccgtcag
    23821 tctcagtccc ggctacaagg gctgccgctc cgtctgcacc aaatgctgca ggaaacacga
    23881 ccttaaagac ctgaaagacc ttgacagact gatccgggta aaccggaaag tcaggataag
    23941 caggctttcg gatgattttt gatttatggg cggtcaatcg ctatatctcg ccgataaatg
    24001 cttccgtttc atattcaaac gatacttttc cgtctgccgc gtatttgtca aagagcattt
    24061 ccagttccgc catcaacggt tcagcattcc tcatttccct gttcggttcg atcccgatcg
    24121 cccggcttcc cctttccagc agcaatgccg taagtatccc ggttcccgag ccgatatcgg
    24181 caatgacgga ctcttccgaa aaccctgcat ttgcgtaaag gtaatcgata aattccttcg
    24241 gatatcccgg cctgtatttt acgtaatttt caacgcgatc tgaaaatctc tcggtacttt
    24301 tcttcaaaac aaaccaccct gttcaaaatt agtgttattg cagccgcttt cctgccagta
    24361 tatccggcga ttatcatttt gtcaatttat tacattcaag tcttttttta tattgtaaaa
    24421 tattatgacc cggccgccgg gccgggtcac aaaattcaat tcctgatctt actcctcatc
    24481 gtcctgttcc cagttgtggt agacgttctg gacgtcgtcc atgtcctcga ggctgtcgat
    24541 gagcttctgc atctgctcta tctgcttcgg atccgtaagt ctcgtcgttg tggtggggat
    24601 catcgacacc tcggcttcca tgaactcata gcccctggat tccagtgctt ccctcacagc
    24661 cgagaagtct ccgggcgcag tgattatttc ataatattca tccccggcgg tcatgtcttc
    24721 ggcgcctgct tccagcgctt ccatcatcag ttcgtcctct gataccttgt cggatttctc
    24781 tatcacgatg acgcccttct tgttgaacat gaacgatacg cttcccgtct ggcccaggtt
    24841 cccgccgaat ttgtcgaaat agtgccggat gtcgccggcg gtcctgttcc tgttgtctgt
    24901 ggcggcttca actatgactg ccactccgcc cggtccgtag ccctcgtaca cgatctcctc
    24961 gtagttcgcg ccatcgcctt ctccggcagc cttctttatg cttctcataa tgttatcatt
    25021 tggcatgttg gctgctttgc acttggcaat aacatccttg agccttgaat tcgaatcagg
    25081 gttcgggccc ccatgcttta ccgcgatagc gatttctctg cccagtttcg tgaatacttt
    25141 tcccttctgg gcatccatct ttccttttct gactctgata tttacccatt ttgaatgtcc
    25201 tgacattatc ttacctccat tgctatattg ccatcttcag gaaatatatt tacacgaccg
    25261 ccgtaatggc ataatatact gatggcagaa tgttaaaata tggtatattt aatgtttaca
    25321 ggaaattact ttctgtaata tatgatacca tatagcgctt aaaagataaa gggtgagctt
    25381 ggtatggtcc tgctgcgcat cgcattggca gccgtagtgg tactggcggc tgcgctggcg
    25441 tttgtttcgc tttgcctgat aaaatcaaac aataatgcaa agctgctgaa aaaacagttt
    25501 gattcagcca tcgaggccaa cagcaggagg accggctttt tttcaagcat ggtgcacgaa
    25561 ctgaaaatgc cgctcagcat aatccttggg gcagcccagc tcatcgagat gaaaagaaaa
    25621 agcgaacgcc aggatgaaaa cggcatggag aaacatatcg ctgcgataaa atgcaattgc
    25681 tacaggatga tgaggctgac gggcaatctc ctcgaccttg caagatcgga agcagggtat
    25741 cttgtgctga agcccgtgaa ctgcgacctg tgcaggcttc ttgaggaaat agtgcggtct
    25801 gtccgtcctt acgcaatgaa aaagcagatc ggccttgtgt tcagaagcgc ccatgacagt
    25861 atagtcgctg ccgtcgatat agagaaaatg gaaaggataa tgctgaacct gctttcgaac
    25921 gccatcaaat tcactgatcc cggcggttcg gtcatagtgt ccgcatactc ggctgacggg
    25981 cgtgtattca tctccgtaaa agatacgggc gtcggcatcc cccctgaccg gcaaaacgac
    26041 attttcggcc tttacagtca ggcagggatc cacagcgcgg aaaaagaagg atccggcata
    26101 ggcctgtacc tggtaaaagc ctttgccgaa ctgcatcatg gaaatgtcag gctcatcagc
    26161 gaagagggaa agggcagcga atttatagta gatctgccat ccggaacggt cacatcctcg
    26221 ccggaagttt cggatgccgg tgaattcggg atcaaggccg gtgatgccgt aagcattgaa
    26281 ttctcgggcg gccattcagt tttaagttaa atgctgtact caaatgcagc aaatcttatg
    26341 atgagagtaa taagcatgca aagctgtgac cggcagcgat catggattat gtgaagcagt
    26401 ccttcggcag tcacaggcaa aagatatagc tcagcagcct tatttcaggt gataatcaca
    26461 cttcttttgt aatatatacc ctcccgcctg cactgatgcg ggctcgtccg tttgacgctt
    26521 ccgtcaccat ggctgagaaa cgatcggatt catcaaccgg aacatatact tccatggcca
    26581 cattatccgt atatatggta tcttttacgc tgtaaccttt cgaagccatt gccgcctgaa
    26641 gctttccaaa gagcgaataa tccgttgtta cgcgggcttc gacgcacagc atcttcctga
    26701 ctattccggc cgcctcaatg cccaatgcgg cgcttttgcc atatgccctc acaaggcctg
    26761 atgcacccag cagcgtgcct ccgaagtacc tcgtaacaac aacggccaca tcctgcacac
    26821 ccgtcttttt tatagcttca agcacaggca gtcccgcagt tcccgaaggt tcgccgtcat
    26881 cgctgaattt ctgcacgaca ttgtccgcac agatatagta cgcataaaca ttgtgcgcag
    26941 catcccaata cctggctttc agcccgttta tgaaatcctg cgcttcctgt tccgaggaca
    27001 ctggttttac ggtggcaata aagcgcgact tcttttcttc gatttcggca gccgcttcct
    27061 tcgaaacggt cagataatcc ttcctcatag tttttacggt tttcctccgt tatattgatt
    27121 gcgcccgttg tattatttca ggatccctcc gccgcaacgg actttcaccg tcaaggtcgc
    27181 gcgcatgccg cgcgtacaac aataaatggc agcattgtct gctgccactt attttaccac
    27241 atagcaaaga taccgtgttg tatttatttg aacaccaccc ggtcctatac ggcaaacgtc
    27301 ccggcgagtg tatccaacac tgctgtcccg gtactttccg gtctttctgg ttctaaacgg
    27361 ctacagatgc aatatccctg tatttcctgt aagacaaatt caaaagcgcc ttgtcctggc
    27421 tgtaagtgac aagtttcatg gcctcgtcga tatcaaaata tcctccgtcg acgaattttt
    27481 catgttcatt gatttcatat tgaagactgt cagtacgcat gatgtaccag atgattttgt
    27541 tacagaccgg tttctgacgt gttacggaat agaactcgta attggtgtga cctgccgttg
    27601 aaattatctc cgccgttatg ccgccttcgg cttttaccct ggcgagagcg acttcgttcg
    27661 gaagttcgcc gtttctgacg acgcctttgg gaaataccca ttctcctttt tcgttcctga
    27721 ggagaaatac cttgttgccg cagaagacta tacctcctgc gcaatttctg actatcatat
    27781 aaaggtccct tcctttcgcc ggccagcttg tgattcgccg ttactctatt attatgatac
    27841 cctaattgca gattttaatc aacttgctag aatgttcatg gtataatttt tatcccgttt
    27901 atccataaaa accatcacaa aaaatatgta tcacgagaca ttactgccat cagccttttt
    27961 aacccagata ttaaagtttt tcctagagca tttggactat accgcatcca tatacacgca
    28021 gcggcatgaa tcacattaca ggcgtaatcg tgcccggcgt cggcaaacca catatcagcc
    28081 caaggctgcg tttcggatcc tgtccgtctc tttgccgtca agtccgtata tcatgcaaac
    28141 catttcatct atctcactga gcagtttttc cgccatggca ccgccgcaga agttttgctg
    28201 cagcttttca gcccgccgcg caagctcctg ttccgtcttt ttatcggtcc ttaccagcgg
    28261 cagctgctca agcatgaact tattgacggc aatatatcct cccgcaagat gcctttcata
    28321 aaacttctct ctcaggtacc atgacatgaa cctgctgttc agcagcgcca gcaaatagaa
    28381 cgggtccgtc cccccaaaac cgtaaatcgc atacgtgccg actccgatcg ccagcggctc
    28441 cctgctgtaa tatgcctcca actcttttgt catccccgcg atgcatatct tttcgctgca
    28501 ccatagtttc cacttgctgt cagctatttt ttcacccttt aagatatacg ggttttcgta
    28561 tatcgtcccc atataccgta ctttgctgcg atcgaggcaa tacctgtcta tagagccgct
    28621 gacagcaaac ggtatagcgt tgccgcatcc gtcgctcatg gggtgttctt ccctcaatgt
    28681 ggcatctgca gcacgttgcg cctctattat gcactctttc aggacggcag cctgaaaacc
    28741 tgcagcgcca gatgcgattt ttatattgtg atccgcaaat gtttcatact gcctggtttt
    28801 tgacctgctg aaaaagcggc gggtctccag ttgctgcagg ctgtctgcgg catactcgag
    28861 caaaccgtca tggctgctgt atctgtcggt gccacatact ccaaattcat ctgtactgcc
    28921 gactccggta catccggtct ttcccggatc gctgcaattc gtatatatgc agcaagaatc
    28981 actatgcacc gaagcacggc agctcccagc atcattccgg gtcctcgtgc cgatgatttc
    29041 ctttttgccc gcttcgcacc tgttccctgt cacaatgacg ggataaactc cgaccccgga
    29101 aaacacattt aagtctgaaa cgcttatggt gtacctgagc ccgttttcct tgagatattc
    29161 caggaccggc atggcatact gcgataccag gagcttgttg ggaactatcc agcagtatac
    29221 tcctttgccg ttaagcctgc ggatcccgtc cagcaggaat gnnnnnnnnn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn natccagcag tatactcctt tgccgttaag cctgcggatc
    29401 ccgtccagca ggaatgcggc atatatgtca aatgagccct tgagctgcgg aaatttcttt
    29461 ttcagtgccc gcctcatccc acggccgctc ctcgatccct ccacggctga aacataaggc
    29521 gggttgccta ttattacatc gaaactgcaa acatcatact caaataggct gtcagcacat
    29581 gaaacattgc atttgaaacc gttctcctct atcgtattgc acgttgcacc tgcatcatgg
    29641 gtcgcgctgt tcatggtttc tgcgtaactc ttcgcgtagc ccggctcttc cgcctcactc
    29701 ctgtaccgtt catattcagt gatcagctca taccaaagcc gaagcctggc gatctctacc
    29761 gctgttgcat cgatatcggc gccatggatg ctgtttgcca cagcattgta cttcagaacg
    29821 aacggatgcc tgtcttccgc catgaacgga cgcctgtccg ggatcggccg gcagcatgca
    29881 gccgtatccg gtgaagtcct gccgctgcag ccgctttgtg cagaaccgtc ccctatgaat
    29941 ctgccggatc tgtcccctgt atatcctgcg gaactgcccc tgatatacca ggtgatgccg
    30001 gacctgagcc tgacgatttc atcgagcatg ccgcatataa atgcgccgca gccgacagcc
    30061 ggatcgaaga ccctgatggt ttcaagtgcc ctgtcggctc caaggagcaa ctcgcggtca
    30121 acgtccgtat tgttttgcac atctgccgtt atacgttcca taaatctcag ggtgagatcg
    30181 aatgaaagtc cggtggaatt ggaaagataa tgcgccaggc tctctctgca catgaacctg
    30241 acaacttcgg gcggtgtgta aaaaacgccg gaaccctttt tatggctgat ttgcagggtg
    30301 tcggtataga gcttcgccag gacatccgga ttaggtatgc ggtctgaaga aacattatga
    30361 aaacgggcag aattgccgga aatgcaggct gtcctgccgg aatcatcatg tacgctgccg
    30421 atcttttcat atatactgcc aaaggcttcc ctgtacctgc ttccccagtc aaaacccata
    30481 ccgcaagtcc tccggcgtta cctgcttccc gtatggccga accctccgcc cctgtcctcg
    30541 ccgatgcttc tcgggtccgg atgcggcacg aattcggccc gcttatgttc gcagaatacc
    30601 atctgcgcga tcctctcgcc cttttttatg tatatggtcc catacggatt gccctgcctg
    30661 tcaatgtaaa ttttctgtcc caataccgca agcggcgcag cgttcgtcac caccggaccg
    30721 caatgccggc ccaggtatgt tccaagatcg acttcctcgt aattttcccg cagttcatcc
    30781 agcagttccg ggttcacagc tacctggtaa gggaggttcg cgatgttgta tgtgttctgt
    30841 attatgatcc cgacagtatc acggtagtcg ctgtcgatcg ttcccgggct gttcggtatc
    30901 cttagttcgg tcctgaccga caggccgctt ctcggcctta tctgggcttc caggccagta
    30961 tcgatggcca tcgaaaaatc cagcggcatt atcctggtct cgcccggcct gatagccata
    31021 tccatcgtcg caaacaggtc gcagcccgca gcatggttag aagcatactg cggtatcttg
    31081 ccctcgcccc agaccttgat atacaccctg actttgttct ccatgcgctc tcctcctct
//
LOCUS       DAOSCC010000004        28857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1197_length_28857_cov_5.107458, whole genome shotgun sequence.
ACCESSION   DAOSCC010000004 DAOSCC010000000
VERSION     DAOSCC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 28857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28857
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1197_length_28857_cov_5.107458"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLG72_00535"
     CDS             <1..358
                     /locus_tag="PLG72_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ79322.1"
                     /translation="NWTGNTVSLLTFCGGVNNEDIYLIYKRDILYITAAAGEVLVTTL
                     QGTYRSRESLNSWIKKLDSGDFFRCHRSYIVNIEKIEKISPWFNGAYNLKLKDCRDII
                     PVSRENMKTLKDIFGI"
     gene            379..2085
                     /locus_tag="PLG72_00540"
     CDS             379..2085
                     /locus_tag="PLG72_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ79323.1"
                     /translation="MDHFSLIDNTVVTEGINRQSAVIITADCIIKAFTADAAEKIRII
                     FGKEIKTGDYIPDLLDPDEKFRLKELVGHALRGSTLRALVKCHGAGNGDSLAELYFSP
                     VYEDVGKATSVCISFWPISESFHDEIRAEHPENKFRELLNNTADLVIVSYITGDGKPG
                     PIVEVNDTVCKILGYTREELLSRRLFELWEHIDKDNVEHIAEELMNGGKVSFEMALFT
                     KNGNRIYTDASIRILEYRHAKVALTVARDITMKKLTEQKLIEINNKLTKALKQLELAK
                     EQLMYSEKLACIGQLAAGVAHEINNPLGFICSNIESTRKNYEDFKEILSEYRDFTAQV
                     PDLSTEGILEGMRRLNVLESEKNLDYMLKDQDDMFDDIDDGLKRISEIVTGLRAFARR
                     SRENAFEEYDLNKSIHNALLLARNDIKYHADVKLNLGVIPMIKAISSRIDQVLLNIIL
                     NTSYAIKEKKMEGPGLITITTDAEGGFVRCRIEDNGIGMDEEVQGRIFYPFFTTKPPG
                     QGTGLGLSIAYDIVVNQHGGQILVESKPMVGSCFTILLPVDRINSEQEICNEQKHIVR
                     RR"
     gene            2054..3241
                     /locus_tag="PLG72_00545"
     CDS             2054..3241
                     /locus_tag="PLG72_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ79324.1"
                     /translation="MNKSILFVDDERPILAALKRVFAGKGFDIFIAESGEEALSFLEK
                     KVTDLVITDVRMPGMNGYQLLKEIRANYPSIIRVVLSGYNDEDLVIRIQRESLAKRYL
                     LKPWKNQELIRTVEQIFSVEEALKKRNLLELVNRIEFLPSPGDICIRLNRLAEQDAGT
                     DEIARTIESDQSLTAKILQVANSSGQEIRTGSVRQAIDYLGLNNVMNILLSAAAFNND
                     RAVSNTCTKKDIEVLWKHAVLTNDILAYLYRRIAGHPIPDMCSMAGLLHDIGKVVLIH
                     NYTGKYLNAAAAIRNMKDMYYYYEKMEFIDITHQEIGAYLLNWWELPQVMVESALYHH
                     DPLDENVTDKELMCLLNAADTCSWNFIYGGGNLTVDPKVLEQLNVTRDDLDRIIRELQ
                     FCR"
     gene            3467..3820
                     /locus_tag="PLG72_00550"
     CDS             3467..3820
                     /locus_tag="PLG72_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79325.1"
                     /translation="MKKVLIVDDAAFMRAAIKTILERNGFEVVGEAENGAIGVRKYQE
                     LRPDIVTMDITMPEMTGLEALKHIRSFDPDAKVIMISAMGQEHLVKEAILSGAKSFIV
                     KPFKEEHVVQTLKKL"
     gene            3842..5914
                     /locus_tag="PLG72_00555"
     CDS             3842..5914
                     /locus_tag="PLG72_00555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOJ79326.1"
                     /translation="MSDQFLGDQMLDMFIFETAQLLEQLEACILDAEKDGAYSRDAIN
                     EIFRIMHTLKGSSAMMRYDDISSLAHSIEDLFYFLREENPGIIEYSELTDLVLAGVDF
                     VKSQIGKIKSGSAADDDASKLIADNREFLAKLKGSDPAKQGADTEKKGPDPGEQEPDT
                     EKQNGTSGRKYINAFRTVIRFQEGCEMENIRAFGVIHNLEDIAGEIRHVPEDIMAPEA
                     ADIIKRDGFTVNFRTDCTYVDIHQLLMQTVFLKELELEQVNEADSAQAINEDRPADYR
                     PSDHRADRTDEHNAQNVSRDHTSHQNIISVPVARLDKLMDLMGEFVIAEAMVIQNPDL
                     DGMELSNFQKAAQRLDKITKEIQDMVMSIRMVPLAATFQKMKRIVRDMCRKLHKEANL
                     VIVGEETEVDKNIIEHISDPLVHLVRNALDHGIESAQERLAAGKDKCGTITLEAKSAG
                     SDVLIIVRDDGRGLDREKILKKAKEKGMLYKPESEMTDKEIFNLVLLPSFSTKDDVTE
                     FSGRGVGMDVVVKNIEAIGGSVLIDSKPNEGTSITMKIPLTLAIIDGMNIKVGKAIFT
                     LPTISIMESFRPKAGDAFTDPEGNEMIMVRGKCYPVLRLNRYYSIPAGITEFTEGIMI
                     MVEHDERTLCIFADELLGQQQIVVKSLPEYIRRFGKVKGLAGCTLLGDGNISLILDIG
                     GLIYGREA"
     gene            5948..6442
                     /locus_tag="PLG72_00560"
     CDS             5948..6442
                     /locus_tag="PLG72_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ79327.1"
                     /translation="MADVMDNTVELEEDTLKGRYLTFSLGNESYGIEVRYVTEIIGMQ
                     AITEVPELPEYVKGIINLRGKIIPVMDVRLRFRKEPKEYNDRTCVIVVDINDISIGLI
                     VDTVSEVLTIPEEDIVEPPQMNKAFSNKYIKHIGKVGNDVKLLLDCEKLLTADEIETL
                     SEVI"
     gene            6471..8309
                     /locus_tag="PLG72_00565"
     CDS             6471..8309
                     /locus_tag="PLG72_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOJ79328.1"
                     /translation="MKWYYNLKIGVRLTIAFLLLALIAAAVGIVGFINLRRIDEADTL
                     LYEENTLGAVYAGNAASYYQRLKYNAAEIILLKDDSQKDDYVEKLNSYIARIDEELKS
                     YEEGIINQEDRQLFDEMAPQWETYKKYMEQIIGYIQNGQYEKAEAILLGESETSGDAV
                     RDSLFKMMEYNQEGARDRAEENSALAGNAETMMIVIIAVAIAFAVFLGIFISRSISKP
                     IAKMVKAADRLATGDVDIQQTIFTKDEIGQLANSFKRLIDSTQAQVTVVERIADGDLT
                     VEVPIRSEKDLLGRKLSEMVHKVNDLLQNIANAAEQVSAGAKQISDSSMALSQGATEQ
                     ASSIEELTASIEQVSSQTKTNAENANKANEMAEQAKNYAVTGNEQMKEMLKAMDEINE
                     SSSNINKIIKVIDDIAFQTNILALNAAVEAARAGQHGKGFAVVAEEVRTLAGRSANAA
                     KETTTLIEDSIKKSGEGTRIAKETAEALAKIVDEVNAVSGLVGDINNASNEQAAAIAQ
                     INQGIMQVSQVVQENSATSEESAAASEELSGQAEILYDLISQFKLKGNAGSRDRNNEL
                     NPEVAKVLNNMSAKAKKDPGAQEKEKQKADAPKAKITLNDMEFGKY"
     gene            8355..9170
                     /locus_tag="PLG72_00570"
     CDS             8355..9170
                     /locus_tag="PLG72_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ79329.1"
                     /translation="MHTITDKEFHQLAEFIKKNYGINLKEEKRALVAGRLQNVLLQKN
                     MKSFSEYFEHVLSDKTGEAAAELVNKITTNYTFYMREPDHFYYFRDVVLPYLVKTVRN
                     KDLRIWSAGCSSGEEPYTLAMIIDEYFRGKKAGWDTRILATDISGRMLEKAKKGIYDY
                     DSIAKLPPQWRLNYFNRLDDNSYSVAGKIRNEVIFRKFNLVDGSFPFKRKFHVIFCRN
                     VMIYFDMKTKDELVERFYNALEYGGYLFIGHSESISRARSRFRYILPAVYRKI"
     gene            9182..10201
                     /locus_tag="PLG72_00575"
     CDS             9182..10201
                     /locus_tag="PLG72_00575"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOJ79330.1"
                     /translation="MPGKRIRVLIVDDSIIFREVLSKGISSDPDIEVAAVAGDPYEAA
                     DKIVRYKPDIMTCDVEMPKMNGIDFIRKLLPQYPMPVIVVSSVSGVVFEAMEAGAVDF
                     VAKPDIQSAKSVEQFIREMIEKIKIASVAKVSTVLARKRLQAGVSKITLNTDKIIVIG
                     ASTGGTEAISCILRQLPENVPGIVIVQHIPPVFSRMFAQRLNETTKLNVKEAESGDYV
                     ERGHALVAPGDRHMKIVRIGDRYKVHCFEGEKVNGHRPSVDVLFESAAKECNDKAIGV
                     ILTGMGYDGAKGLLSMRKKGARTIGQDEASSVVYGMPRVAYNIGAVEIQASLERIPQV
                     ICSLL"
     gene            10433..11557
                     /gene="cobT"
                     /locus_tag="PLG72_00580"
     CDS             10433..11557
                     /gene="cobT"
                     /locus_tag="PLG72_00580"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOJ79331.1"
                     /translation="MDGKEKNLARYIAAITDIDHAAADAARKRLDSLAKPPGSLGRLE
                     DIAVQLAGITGQTCNTLDKRCVIIMCSDNGVVEEGVASAPQSVTYAQAINFTRGITGV
                     AVLAKCFNSDLMVVDVGINAEVNHPGIINRKIRKSTSNLANQPAMSRGEAVTAILTGI
                     ETAREAHRKGYRMIGVGEMGIGNTTTSSAVLCALTGIDAGLAVGKGAGLTSEGFEKKK
                     RIIMDAIKTYKPDPNDPIDVISKVGGFDIAAMAGVYIGAAYCRLPVVIDGFISAVAAL
                     AAARMASLSVGYMIPSHASCEPGYAYTVRELGLEPVLDLGMRLGEGSGCPIMFMVIDA
                     ACAVIRDMGTFAEAVIDGRYLENIREEGCFSTDGRKGAEC"
     gene            11551..12114
                     /locus_tag="PLG72_00585"
     CDS             11551..12114
                     /locus_tag="PLG72_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOJ79332.1"
                     /translation="MLRIFISGGCKNGKSYIAQRLAKGQAKGRLYYVATMRPCDGEDA
                     ERVERHRQERDGWGFETVEQPIDILKCLDSCDADASFLIDSTTALLANEMFPAGNSVN
                     MDAPRKIAGELAGLCRQVKDIVIVSDYIYSDAMLYDELTEAYRMGLAYIDRTLAAECD
                     VVLEVAFGCITVHKGREVFRPLYEKAV"
     gene            12125..12862
                     /locus_tag="PLG72_00590"
     CDS             12125..12862
                     /locus_tag="PLG72_00590"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOJ79333.1"
                     /translation="MSLGMFCAIPLPYTKWEENCANLVIPFFPAVGLIIGALWYGAAR
                     LLLLAGLGKVMLSAFLMIFPYAITGFLHIDGFMDASDAILSRRSREEKLRILKDPHAG
                     AFAVVSVLVLFVICFASTYEIVSGTSAAGKLRFLLFIPAISRSLAGAALLSLKALPQS
                     SYGSYFKKNVKTVHKIVVVLPGIVAAAAVCLLLGIKGSIAVGAMAAGFSISIIHAYRQ
                     LGGVSGDLSGYALTVSEACGLVALALV"
     gene            12874..13251
                     /locus_tag="PLG72_00595"
     CDS             12874..13251
                     /locus_tag="PLG72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOJ79334.1"
                     /translation="MVLVFGGAYQGKLEYVIRNYGIQENEIFHCDDASEIDFSKRAVY
                     GLEKLVLNLIRCGADPLDYINMNLDKINDKIIICTDISAGVVPTDPVMRQWREAVGRC
                     MTLLSRNSERVVRIFCGLGTVIK"
     gene            13248..13847
                     /locus_tag="PLG72_00600"
     CDS             13248..13847
                     /locus_tag="PLG72_00600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ79335.1"
                     /translation="MNALIHIIRHGRTFANTNGLYCGSTDVSLSETGIREILEYKEKG
                     IYPEAELFITSGLKRTAETLRLIYGDVDYITVAGLREYNFGEFEMHSHYELEHRNEYQ
                     AWITDEAGDVCCPCGESRIGFCKRVEQGINDVLDEMERRRAGSAAVICHGGVIMAIMH
                     ILFPGERNYYEWQPDNGLGYTLQYSDGNFSGYRSIDPCK"
     gene            complement(14131..14221)
                     /locus_tag="PLG72_00605"
     tRNA            complement(14131..14221)
                     /locus_tag="PLG72_00605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14184..14186),aa:Ser,seq:gga)
     gene            14491..15462
                     /locus_tag="PLG72_00610"
     CDS             14491..15462
                     /locus_tag="PLG72_00610"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ79336.1"
                     /translation="MKSRSAFSNLRITNVTRKRPVRRIISIILILLAVIVIAGFAAIP
                     PLVMKYLILLHADVSEMPPEQFGVEAEEVWLTTEDGFRLVSWEVKASKPRGIVIFLSG
                     IHNPSVTAYFGHAKMMADNGYSSLLIEMRSHGRSDGDKIYAGTREYLDVKAGVEYIKT
                     RPEYNDLPVIVFGVSMGGATAINSAGEIADIDGLISISAFSSWEDAFADNMANMGFPP
                     IIVKMEKPFVKLYMCFVYGFGSIKISPVNEIKKLNGRPALLMHSTEDSQVPYDSFERL
                     KEAAPEQVETYVRTGDYHMMCMEEYFREPWKDEEYAGAILSFLKKYF"
     gene            complement(15495..16256)
                     /locus_tag="PLG72_00615"
     CDS             complement(15495..16256)
                     /locus_tag="PLG72_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79337.1"
                     /translation="MSKINRYVKEVLSYIIADSKMKERIREDLTSQLNEAARTEDIDS
                     VIARMGDPKQVAREFMDSIYENKSEFFDELLSDISENAKYLKPIYEYKSQATVFGIPL
                     VHVKINRYGRPTRSKGIISISTLPVGVVSIGAIPIGIISIGGLALGVIAFGGLAFGLL
                     LALGGVAAGTAAFGGVAVGLGAFGGVALGKIAIGGVASGTVAIGNEIDGDYMLKISRL
                     TPQTCAEAAALIRAAFPELPDWIVRLFSGIAKFLR"
     gene            complement(16402..16734)
                     /locus_tag="PLG72_00620"
     CDS             complement(16402..16734)
                     /locus_tag="PLG72_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ79338.1"
                     /translation="MTEVSKQLKKGVIEILILSLLREEKMYGYQLIQELDARSCGVFK
                     MKEGTLYPVLYRLEDSKYIDSCWEQDHERRSVPRKYYMITDEGIKALENMKQELETLI
                     YAIRLVLR"
     gene            16987..17538
                     /locus_tag="PLG72_00625"
     CDS             16987..17538
                     /locus_tag="PLG72_00625"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOJ79339.1"
                     /translation="MNSIYFYDTPVGRAAIADNGRAVIQLKFIREGWISGDRLGRSET
                     GAADVWPAGSCGTPFIICETPLIKEAARQLYEYLDGNRKVFDIPLELEGTPFQKAVWQ
                     ALLEIPYGETRSYGEIAERIGNPKAARAVGMANNRNHVAVFVPCHRVIGADGRLVGYA
                     SGIDIKKKLLDLEKLYTTKKPSL"
     gene            17740..18663
                     /locus_tag="PLG72_00630"
     CDS             17740..18663
                     /locus_tag="PLG72_00630"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOJ79340.1"
                     /translation="MNVPAYDEKNKTGASLHIGCHLSSSEGYLRMGMTALSIGADTFQ
                     FFTRNPRGGKAKGIDPDDVAAFLKLAEENGFVQIMAHAPYTLNPCSADKRVRAFALEV
                     MSDDLARMEYIPGSFYNFHPGSHVGQGIEAGIALTADLLNRILKPDQTTTVLLETMSG
                     KGSEIGGRFGELRQIIDKVELKEKLGVCFDTCHVYDAGYDIVNDLDGVLEEFDEIIGL
                     ERLRAVHMNDSKNPFYSRKDRHAKIGEGSLGIETFANLVNHPKLRHLPFYLETPNELE
                     GYAQEIRLLKEMRHIIPLPGMPDHDSMCNGE"
     gene            18665..19375
                     /locus_tag="PLG72_00635"
     CDS             18665..19375
                     /locus_tag="PLG72_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOJ79341.1"
                     /translation="MQEMKDLQGKIKARLKELSEGKYREFASGLLPDVNNMLGVRLPV
                     LRSVAREIAKDDWRGYLETADDEFFEEIMLQGFIIGLAKTDVEEKLRLIREFVPKIGN
                     WSVCDSFCASLKFVNSNKERVWDFLQQYFNSEREFELRFAIVMGIFYYTDDDYMDEFL
                     RLLARIRHDGYYVKMAMAWALSVCFVKRPGKTLEYLKNSEFDDFTYNKALQKIIESKM
                     VDDENRAMIRGMKRKTVD"
     gene            19442..20014
                     /locus_tag="PLG72_00640"
     CDS             19442..20014
                     /locus_tag="PLG72_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ79342.1"
                     /translation="MTRISKDPEERREEIIETAQKLFEEKGYEHTMMSDISQTLGISA
                     GLPYRYFRSKLDLLDAVAARLGKEFVRSLIGYRFKPGMNAKGKLDEYFRIIEDMGATK
                     LVSLLHRKDNSEIHKRISEASFRSLMPQLIELIREGNSQKVFDCPYPEETAAFLMYGA
                     MSVHDMVNTENMHEKMEIIRELFYRVLGVK"
     gene            20277..20978
                     /locus_tag="PLG72_00645"
     CDS             20277..20978
                     /locus_tag="PLG72_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79343.1"
                     /translation="MSYITFENVTKEYRSGENAVLAADNANFAIEKGELCVILGPSGA
                     GKTTVLNLLGGMDRATSGRIQVGGREITSFNEEELTAYRREAVGFVFQFYNLMPNLTA
                     LENVELAKQICKNPLDPVEVLEQVGLGDRMDNFPSQLSGGEQQRVSIARALCKNPALL
                     LCDEPTGALDSRTGMQVLKLLSDVSAKYGTTVVIITHNTLIAPIAGRVIRMKNGRIES
                     VEKNNAPVPVEEIAW"
     gene            20972..23431
                     /locus_tag="PLG72_00650"
     CDS             20972..23431
                     /locus_tag="PLG72_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79344.1"
                     /translation="MVKNRLFTKFLRDMRHSKGQLLSVMLLSMLGVWVFSGLDIYWRN
                     LDVSIESYFDEQKLADFWVITPLADKDPEEKIRSVDGVEDLMSRLSYEVSTAVPEDAK
                     LVLHAVGDDIDINIPRITSGGPLWDSDSDGCLLDVRFAEANGLAHGDSIVLKIGEEKR
                     EFVIRGLVMSPEHVYIAPDVIPEPKIYGFAYVNKAAFPNIPSNEICVLLSDDADTEEA
                     KAAIEDKLPGAFIQDRRSHKSTQLISTEVSQFRSLSKVFPVMFFAVSALIVMTAMTRI
                     VSNQRTQLGLLKALGYGKGTIVLHYMSFGFFPSLGGAAGGLALGCNTLPAFLWNILDD
                     IYILPDVKTAPLSAATLTVCMSSVLLTCIICWCACRRSAAETSASLLRPKAPASGSRI
                     LLERISPIWERLSFSAKMIQRNLLRNKIRTIMALSGVLSCTALIITALGLLDSVDHMV
                     DTYYGETLKFDLRADLDEGAGSAGEYRRLIDAGKVEGIMEMAASIRKTDGNGSRAVLL
                     TVVENDHELLSLDTAGTSPGTEETGKNGRNTRQSYPADSYADDKYRIGIQDNRVQHDM
                     PDTGAEYETEIRHRESVIASEKLAQVMGISAGDILKLKLPGAAEEVEVFVERLVPVPI
                     GQGIYMYERSWNSLKKGEFTPTALLIRNPCKEVYDLLEGLDEVTCIKSLQTLRDKTKE
                     GLESLTGVSVLMVAFALVLAFVVLYNMGILNFMERVREFATLKVLGFRHREIRSLIVK
                     ENIIIAVTGILAGIYPGVRLTDIVMKSSEPEDMVFSAVAEPTSILAACCITFVFSLLV
                     QYMLAGKVRSIVMTEALKIVE"
     gene            23675..24304
                     /locus_tag="PLG72_00655"
     CDS             23675..24304
                     /locus_tag="PLG72_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ79345.1"
                     /translation="MVATDERATLKVWRRDVVNSRSSRKLRKAGFVPASISIRGKDSI
                     SVKIKTDELMKNLRKYGRNHLFNLDIDGNETYTAIVKGMQYSSVSRELMDVSFQHVSL
                     DEEIKVEVEIKVTGREAAESRRLLVVTQLDKIPVKGLPQNIPEYLEIDVAKLNADDKI
                     TVGDVRYPEGIVPDCDTEKIVLVITEAKSGAAAESQEGQEEQEDQKAAE"
     gene            complement(24475..25926)
                     /locus_tag="PLG72_00660"
     CDS             complement(24475..25926)
                     /locus_tag="PLG72_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ79346.1"
                     /translation="MTQATAEINENRATDKNKMGAMPVGRLIITMSLPVMLSMLVQAL
                     YNFIDSVYVSRINENALTALSMAYPAQYLMIAVMTGTGVGVNALLSKSLGEKNRERAS
                     QAANTGIFLGIAGSILFAVLGILFTRYYFTSQTEFQEIIDYGTEYLSIVTIFSFGLAG
                     QVTFERLLQSTGKTHLSMITQCCGAVLNIILDPILIFGWLGLPAMGIKGAAVATVISQ
                     CTAAALAFVLNISLNSEIKLKIAEIRPRAEIIKRIFAVGIPVAVMMSISSVMIYGLNR
                     ILIAFTPTAIAVFGAYSRLEHFLFMPVFGLSTSIVSIISYNYGAGSKRRILQAIRLSI
                     LYATVVMAIGFALFQAFPGHLLSMFKASETMLEIGVPAFRIISISFIFAGFCVVSGSV
                     FQALGSGLPSMLESLARQLLALLPLAYVFAKTGGLDAVWWAYPLAEIVSVAMCIAFLR
                     YFYVKRIKPLPDPEPEQQHETTYDGLSRRKIRP"
     gene            complement(26063..27157)
                     /locus_tag="PLG72_00665"
     CDS             complement(26063..27157)
                     /locus_tag="PLG72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6076 domain-containing protein"
                     /protein_id="HOJ79347.1"
                     /translation="MRFVFRNNRTLIIDNVSMPIGSAFAEFINIDFVQAAELFRTGYI
                     ESIESESSWDRNIVRKYYEIFLSSLCKIHPYFFAYADYLKSEIDRRLRESFVHINRSD
                     SRELDDGEKKKVKLVFNNVCLSASKLLDTAKKVQNIFAKAAHHCLDIENAGQGKTPID
                     CLYEYGVDRISISKANQFETGSNIFSQTEIEQVSVPGMTYEIDDSGGDSVISEVWDIK
                     DVYGICYIEFFKMVSGNIMIKKCSNCKKYFLPSNRIDTAYCDRVVTDNGKTCKDIGAI
                     KTFQEKVRTDPALAAYIKAYQSMYARTVKPCRSDPGKKKEAKKQLRQWSYAAQQELEK
                     TRKGAITYEQFTDWLNQNVSVSPAPAGRHA"
     gene            complement(27386..27462)
                     /locus_tag="PLG72_00670"
     tRNA            complement(27386..27462)
                     /locus_tag="PLG72_00670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27426..27428),aa:Arg,seq:cct)
     gene            27692..>28857
                     /locus_tag="PLG72_00675"
     CDS             27692..>28857
                     /locus_tag="PLG72_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ79348.1"
                     /translation="MIYGRYTGKPREKEQDPLNINKTARNNFIRTFLFLFIIMVVFMA
                     ISHYADAFDPGFNAYPLRPDTDTKMQLEWLSVPNAEFYRIFRYDESINDFTLIKSINV
                     GGTLDPFSYTDQGLKPETTYRYMLVAYDANRKVILSDEATNTTTAMIRPHGLSAVFDV
                     TSRKALLTWKSSALAEGSMINMTENGVSVPPFPLDIPETTSYTVEILGSNPVQFTVRT
                     RSGAYGTSALSDPVTVVPVALPYLTAEYVNESTVKISWGSSTNIDVYVLERSKWDGAS
                     WGTWETVSTTLSGTYMTQTVEEGGQYRYRLRAREDKGYTGNSNVTNYVNNLVAPKDLK
                     GKITAEDQIDLSWTNPPGNTGRLEVWRKAGGSSTSGQYTLVGTLNTNDTTFSDK"
BASE COUNT         8099 a         6484 c         7655 g         6619 t
ORIGIN      
        1 taactggaca ggcaataccg tcagcttact gacattttgc ggcggcgtaa acaatgaaga
       61 tatctacctg atatacaaga gagacatttt gtacattacg gctgcagcag gcgaggtact
      121 tgttactacc ctgcaaggta cgtacaggag ccgcgaatcg ctgaattcgt ggatcaaaaa
      181 gctggacagc ggtgacttct tcaggtgtca cagaagctac atagtgaata ttgaaaaaat
      241 agaaaaaata tctccatggt ttaatggcgc ttacaactta aaacttaaag actgccggga
      301 catcataccc gtgagcagag aaaatatgaa aaccctgaaa gacatattcg ggatatgatt
      361 tagccggagg cggcatagat ggaccatttc agcttaatag acaatacagt cgtgacagaa
      421 gggataaacc gccagtctgc tgtcatcatt acggcagact gtatcataaa ggcttttacc
      481 gctgatgctg cggaaaaaat aaggatcata ttcggcaaag aaataaaaac cggcgactac
      541 atacctgatc tcctggatcc tgatgaaaag ttcagactta aagaacttgt cggacatgct
      601 cttcgcggca gcaccctgag agctttggta aagtgccatg gcgccgggaa cggagacagt
      661 ctggccgaac tgtatttcag cccggtttat gaagacgttg gcaaagcgac gagtgtctgc
      721 atttcttttt ggcccataag cgaatccttc cacgatgaaa taagagcaga gcatcctgaa
      781 aacaaattcc gcgagttgct caacaacacg gctgacctgg taatcgtctc ctatattacg
      841 ggcgacggga aaccgggccc gatagtagaa gtcaatgaca ctgtctgcaa aattctcggg
      901 tataccaggg aagaactgct ttccaggcgc ctattcgagc tctgggaaca tattgacaag
      961 gataacgtgg aacatattgc tgaagagttg atgaacggcg ggaaagtgtc ttttgagatg
     1021 gctcttttca caaaaaatgg aaaccggatc tatacggacg ccagcatccg cattctcgaa
     1081 tacaggcatg cgaaagtagc tctgactgta gcaagggaca ttacaatgaa aaagctcact
     1141 gaacagaaac tgatcgaaat aaacaacaaa ctgacgaaag cgctcaaaca actggaactg
     1201 gctaaggagc agcttatgta cagcgagaaa ctggcgtgca taggccagct tgcagcaggc
     1261 gtcgcacatg aaataaacaa tccccttggc tttatatgca gcaatatcga aagtaccagg
     1321 aaaaactacg aagacttcaa agaaatcctg tccgaataca gagattttac cgcgcaggtg
     1381 ccggaccttt ccacggaagg catccttgag ggaatgcgga ggctgaatgt cctggaaagc
     1441 gaaaaaaacc tggattacat gctgaaggac caggatgaca tgttcgatga tattgatgac
     1501 ggcctgaaga ggatcagtga gatcgtcacg ggactcaggg catttgcaag gcggagccgg
     1561 gaaaatgcat tcgaggaata tgatttgaac aaaagtatac ataatgctct gctcctggcc
     1621 aggaacgata taaaatacca tgccgacgta aagctgaatc ttggtgtaat cccgatgata
     1681 aaggcaataa gcagcaggat cgaccaggtc cttctgaata tcatcctcaa tacgtcatac
     1741 gccatcaaag aaaagaaaat ggaaggacct ggactgatca cgataacaac ggatgcagaa
     1801 ggcggatttg tacggtgcag gatcgaagac aacggcatag gcatggatga agaagtccag
     1861 ggaagaatct tctatccgtt ttttaccacg aaaccccccg ggcagggtac agggctcggg
     1921 ctcagcattg cctatgatat cgtcgtgaat cagcatggcg ggcagatact tgtggaaagc
     1981 aagccgatgg tcggctcatg ctttaccata ctgctgccgg ttgaccggat caattcggaa
     2041 caggagatct gcaatgaaca aaagcatatt gttcgtagac gatgagaggc cgatactggc
     2101 tgcgctgaaa agagtgttcg ccggcaaagg ttttgatatt tttattgcgg aaagcgggga
     2161 ggaagcgctg tccttccttg agaagaaagt aacggatctt gtgatcaccg atgtcaggat
     2221 gccgggaatg aacggctatc aactgctcaa agagatcagg gcaaactacc cgtcgatcat
     2281 aagggtggtg ttgagcggct acaacgatga agacctggtg atccggatcc agagggagag
     2341 ccttgcaaaa cggtatcttt taaaaccgtg gaagaaccag gaactgatac ggactgtcga
     2401 gcagatattc agtgttgaag aggcccttaa aaaaagaaac cttttggaac ttgtgaacag
     2461 gatcgaattc cttccttcgc cgggcgatat ctgtatccgg ctgaacaggc tggcggagca
     2521 ggatgccggt accgatgaga tagcaaggac tatagagtcc gaccagtctc tcacggcgaa
     2581 aatactccag gtggccaatt catcgggcca agaaattagg accggatccg taaggcaggc
     2641 gatcgattat cttgggctca acaatgtaat gaacatactg ctgagtgcgg cagcattcaa
     2701 taatgacagg gcggtcagca atacgtgcac gaagaaagac atcgaagtgc tctggaaaca
     2761 tgcggtatta acaaatgata tcctggcgta cctttacagg cggatcgcag ggcacccaat
     2821 acccgatatg tgttccatgg caggattgct tcatgacatc gggaaggtcg tactgataca
     2881 taactatacc ggcaaatacc tcaatgctgc cgcagccatc aggaacatga aagatatgta
     2941 ttactattat gaaaaaatgg agttcatcga tataactcac caggagatcg gcgcatatct
     3001 tctgaactgg tgggaacttc cccaggtcat ggtggagtcg gcgttatatc atcacgatcc
     3061 cctggatgaa aacgtgacgg acaaagaatt gatgtgtttg cttaacgctg ctgacacctg
     3121 ctcctggaac ttcatttacg ggggcgggaa tctgactgtc gacccgaaag tactggagca
     3181 gcttaatgtt acaagggatg acctggaccg gatcatcagg gaattgcaat tctgcagata
     3241 accggactga atgccgttca tcatggaaaa tctaccattc atacccgatt ttctacattt
     3301 cacgggaaaa atcacaaatt caattcgcaa tgttatatta ttacgctgac tggcaggctt
     3361 atccatatac tgatatttca atctgctgat aaacctgcac aagaggttct gtgggacaaa
     3421 aaaatcgatt aatccaatcc gatatgatgc cgaggtggca taaacgttga aaaaagtact
     3481 catcgtagac gacgctgctt ttatgagggc tgctatcaaa accatacttg agagaaacgg
     3541 ttttgaagtg gtcggagagg ctgaaaatgg cgcgataggg gtcagaaaat accaggaatt
     3601 gcggcccgac atcgtcacca tggatatcac catgccggaa atgacaggtc tcgaggcgtt
     3661 aaaacatatc cgcagtttcg atcccgatgc gaaagtaatc atgatatcgg caatggggca
     3721 ggaacatctt gtaaaagaag ccattctaag cggagccaaa tcatttattg tcaaaccgtt
     3781 taaagaagaa cacgttgtcc agacgctgaa aaaattatag atctgaaaga ggatgatagg
     3841 tatgtcagat cagtttttag gcgatcaaat gctcgatatg ttcattttcg agacggccca
     3901 gctgctggag cagcttgagg catgcatcct ggacgccgaa aaagacggcg cctactccag
     3961 ggacgcaatc aacgaaatat tccgcatcat gcacacattg aaagggtcct ctgcaatgat
     4021 gagatacgat gacatctcgt cgcttgccca ttccatcgag gacctgttct acttcctccg
     4081 cgaggaaaac cccgggatca tcgagtattc ggaactgacg gacctggtac ttgccggagt
     4141 cgattttgta aaatcccaga tcggaaaaat caaatccggg tccgcagcag atgacgacgc
     4201 ttctaagctg atagcggaca acagggagtt tctcgcgaaa ctgaaagggt cagatcctgc
     4261 aaaacaggga gcagatactg agaaaaaagg acccgatccc ggggaacagg aaccggacac
     4321 tgaaaagcaa aacggcactt ccggccggaa atatataaat gcattccgca ccgtcatccg
     4381 tttccaggaa ggctgtgaaa tggagaacat cagggctttc ggcgtaatac acaacctgga
     4441 ggatattgcg ggagagatcc ggcatgtacc ggaagatatc atggcgcctg aggctgcgga
     4501 tatcattaag cgcgacggct ttaccgtcaa tttcaggact gactgcacat acgtggatat
     4561 acaccaactg ctgatgcaga cagtattcct taaagaactg gaactggaac aggtaaatga
     4621 agctgattcc gcacaggcga tcaatgagga caggccggcc gattacaggc catctgatca
     4681 cagggccgac aggacggatg aacataatgc ccaaaacgtc agccgcgacc atacgagcca
     4741 tcagaatatc atcagcgtac cggttgcaag actggataag ctgatggatc taatggggga
     4801 gttcgtgata gcggaagcga tggtcatcca gaaccccgac ctggacggca tggaactgag
     4861 caatttccag aaagcagccc agcggctcga taagataaca aaagagatac aggatatggt
     4921 catgtccatc aggatggtcc cgctggcggc gactttccag aagatgaagc ggatcgtgcg
     4981 tgatatgtgc agaaagctcc ataaggaagc gaatctcgtt atcgtcggcg aagaaaccga
     5041 ggtcgacaag aacataatcg agcatatctc agacccgctg gtacatcttg taaggaatgc
     5101 gctggaccat gggatcgaat ccgcacagga aaggctggcc gccggaaagg ataaatgcgg
     5161 gaccatcacg ctggaagcga aaagcgccgg aagcgatgtc ctgatcatag tacgagacga
     5221 cggcagaggc ctcgacaggg aaaagatcct gaagaaagcc aaagaaaagg gcatgctcta
     5281 taaacccgaa tctgaaatga ccgacaagga aatattcaat ctcgtattgc tgccgagctt
     5341 ttcgaccaaa gatgatgtga ctgagttctc aggccgtggc gtcggcatgg atgtggtcgt
     5401 gaagaatatt gaagccatag gcggctcggt gctcatcgac agcaagccga atgagggcac
     5461 atcgataacc atgaaaatac cccttaccct tgcgatcatc gacgggatga acataaaggt
     5521 cgggaaggct attttcactc tgcccaccat ttccataatg gaatccttca ggccgaaggc
     5581 aggggacgcg ttcaccgatc ctgaaggcaa cgagatgatc atggtgagag ggaagtgcta
     5641 tccggtcctg aggctgaacc ggtactacag catccctgcc ggcatcaccg aattcacgga
     5701 aggcatcatg ataatggtgg aacatgatga aaggacactt tgcatatttg cggatgaatt
     5761 gcttggccag cagcagatag ttgtcaaatc cctgcccgaa tacatcagga ggttcgggaa
     5821 agtgaaggga ctggcagggt gcacgttatt gggagatggg aacatcagtc tgattctgga
     5881 tatcggaggg ttgatatatg gccgggaagc atgacgacta catcgaagaa aggaaggaga
     5941 tgatggtatg gcggacgtga tggacaacac tgtcgaactg gaagaggata cgctaaaagg
     6001 caggtacctg acattttcac tgggtaacga gagctacggc attgaagtaa ggtatgtgac
     6061 cgaaatcatt gggatgcagg caattaccga agtgcctgag ttgcctgagt atgtcaaagg
     6121 gatcatcaat ctgagaggca agataatccc ggtaatggat gtaaggctca ggttcagaaa
     6181 ggagccgaag gagtacaacg acaggacatg tgtgatcgta gttgacataa acgatatatc
     6241 gataggtctg atcgttgaca cggtatcgga ggttcttacc attcctgagg aagacatcgt
     6301 tgaaccgccc caaatgaata aagccttcag caataagtac ataaagcaca tcggcaaagt
     6361 cgggaacgac gtcaagctgc tgctcgattg cgaaaaactg cttaccgctg atgagatcga
     6421 aactctgagt gaagtaatat gagaatcaac atgtaaggag ggtaaattac atgaaatggt
     6481 attataattt aaaaatcggc gtaaggctga ccatagcttt tctcctgctt gctttaatag
     6541 cagctgcggt aggtattgtt ggctttatca atcttcggcg gatagatgaa gccgatacgc
     6601 tcctgtatga agagaatacg ctgggagccg tctatgcggg caatgccgcg tcatattacc
     6661 agagactgaa gtataacgca gcggagataa tcctgctgaa ggacgacagc caaaaagacg
     6721 attatgtgga aaagctcaac agttatattg caaggatcga tgaagaactt aaatcgtacg
     6781 aagagggcat catcaaccag gaagaccgcc aacttttcga tgaaatggcg ccgcaatggg
     6841 aaacatacaa aaaatacatg gaacagatta tcggatatat tcaaaacggg cagtatgaaa
     6901 aagctgaagc catactgctg ggagagtcgg agacttccgg ggatgcagtc cgtgattccc
     6961 tcttcaaaat gatggaatat aaccaggaag gcgccaggga cagggcagaa gagaattcgg
     7021 cattggccgg aaacgccgaa acgatgatga tcgtcataat cgcagtagcc attgcttttg
     7081 ctgttttcct tgggattttc atatcccgtt ctatcagcaa gcccatagca aaaatggtca
     7141 aggcagccga caggcttgcg acaggtgacg tagatatcca gcagaccata ttcacaaaag
     7201 atgagatagg acaactcgca aattcattca aacgccttat tgacagtacc caggcccagg
     7261 taacggtcgt tgaacgtatt gccgacggag accttactgt ggaagtcccg atacggtccg
     7321 aaaaagactt gctcggccgc aaactctccg agatggtaca caaagtaaac gatctgctgc
     7381 agaatatcgc caatgccgct gaacaggttt cagccggggc aaagcaaata tccgattcca
     7441 gcatggcgct ttcccaggga gcaactgaac aggcaagctc gatagaggaa ctgaccgctt
     7501 cgatagaaca ggtatcgtcc cagacaaaaa caaatgcaga aaatgcaaac aaggccaatg
     7561 agatggctga acaggcgaaa aactatgcgg ttaccggaaa tgaacagatg aaagaaatgc
     7621 tgaaggcaat ggatgaaatc aatgaatcgt ccagcaatat caataagata atcaaagtta
     7681 tagatgacat agcattccag accaacatac ttgcgttaaa cgcagcagtc gaagcggcaa
     7741 gggccggtca gcacggcaaa ggctttgccg tagtcgccga agaggtcagg acgcttgcag
     7801 gacggtcggc taatgcggct aaagagacga caacactgat cgaagattcg ataaagaagt
     7861 cgggagaagg taccaggata gcaaaagaaa ctgctgaggc tctagcaaag attgttgatg
     7921 aagtcaacgc cgtatcaggc ctggtcggcg atataaacaa cgcttcgaat gagcaggctg
     7981 ctgcaattgc ccagataaac cagggcatca tgcaggtatc gcaggtcgtc caggagaatt
     8041 cggcaacgtc tgaggaaagc gctgctgcga gcgaagagct ttccggccag gctgagatac
     8101 tgtacgatct gatcagccag ttcaagctca aggggaatgc agggtcacgc gacaggaaca
     8161 atgaactgaa tcctgaagtg gcaaaagtgc tcaataatat gtcagcaaag gcgaaaaaag
     8221 atcctggcgc gcaggaaaag gagaaacaga aagctgatgc tcccaaggcg aaaatcacgt
     8281 taaatgacat ggaattcggc aaatattgac gcgcataata tttgccataa taatcgataa
     8341 cggacgtcca acagatgcat accattaccg acaaggagtt tcaccagtta gctgaattta
     8401 tcaaaaagaa ttacggtatc aatctgaaag aagagaaacg tgctctggtc gcaggaaggc
     8461 tgcagaatgt tcttttacaa aaaaatatga agagcttttc cgagtacttt gagcacgttc
     8521 tttcagataa aaccggagaa gcggccgccg aactggtcaa caagattacc accaactata
     8581 cgttctatat gagggagccc gaccatttct attatttcag ggatgtcgtg ctcccatacc
     8641 tggtaaagac ggtgagaaac aaagatctca ggatctggag cgcaggctgc tcatccggcg
     8701 aagaaccgta tacgcttgca atgatcatag acgaatattt ccgcgggaaa aaggccggat
     8761 gggacacaag gatactggct acggatattt ccggcaggat gctcgagaaa gcgaaaaagg
     8821 gcatatatga ttatgatagt atagcaaaac tgccgccgca atggagactg aactacttca
     8881 accggctgga tgacaacagt tattccgtgg ccggcaaaat caggaatgag gtcattttcc
     8941 gaaaattcaa tctcgtggat ggttcgttcc cgtttaagcg caagttccat gtgattttct
     9001 gcaggaacgt gatgatctac tttgatatga aaacgaagga cgaactcgtg gagcgcttct
     9061 ataacgcgct ggaatacggg ggatatctgt tcatcgggca ttccgaatcc ataagccgcg
     9121 ccaggtccag gttcaggtat atattgccgg cagtctacag gaaaatttag gggagaaaaa
     9181 tatgccaggg aaaagaatac gagtattgat agtagatgac agcataattt tcagagaagt
     9241 cctgtcaaaa gggatctcct ccgacccgga catagaagtg gccgccgtcg caggggatcc
     9301 ctatgaggct gcggacaaga tagtaaggta caaaccggac atcatgacat gtgacgtgga
     9361 aatgccaaaa atgaacggca tcgatttcat ccggaagctt ctgccgcaat accccatgcc
     9421 cgtcatcgta gtgagctccg tcagcggggt cgtgttcgaa gcaatggagg cgggagctgt
     9481 agatttcgtc gcaaaacctg acatccagtc cgcaaaaagc gtcgagcaat tcatccgcga
     9541 gatgatagaa aagatcaaga tcgcgtctgt ggcaaaagtg tcgacggtac tggcgaggaa
     9601 acgcctccag gccggtgtga gcaagatcac tttaaacact gacaagataa ttgtcatcgg
     9661 agcttcgaca ggcggcacgg aagcaataag ctgcatcctc aggcaactgc ctgaaaacgt
     9721 gcccggtatc gtgatcgtgc agcacatacc tcccgttttt tcccgcatgt ttgcccagag
     9781 gctgaacgaa acgacgaaac tgaatgttaa ggaagcagag agcggggatt acgtcgaaag
     9841 gggccatgcg cttgtggcgc ccggagaccg ccacatgaaa atagtcagga taggggacag
     9901 gtacaaggtc cactgctttg aaggggaaaa ggtgaacggg caccggccgt ccgtcgacgt
     9961 cctctttgag tccgcggcga aagaatgcaa tgataaagcg ataggagtca ttctcacagg
    10021 gatgggttac gacggcgcaa aaggcctgct ttcaatgagg aaaaaaggcg cgcggaccat
    10081 agggcaggac gaagcatcat cagttgttta cggcatgcca agagtagcat acaacatcgg
    10141 cgccgtcgag atacaggcat ccctggaaag gatcccccag gtgatttgct cattgttgtg
    10201 aaaacagtgc aaagctcaac catgcctttt gctgcaagaa ccgataagcc aaagctgaac
    10261 cggtcgacat aacctccgcc aaatgtactt tatactgaaa gaattttttc aaatacgtaa
    10321 gcgccatctg atatacatgg cgctgaaatt ttattgtata attatatcgg ccttatgaag
    10381 aaccctccgg aagatccgcc ggatccagca accataggtg aggagaacag gcatggacgg
    10441 aaaggaaaaa aatctggcaa ggtacatcgc ggccataacc gatatagatc acgccgcggc
    10501 cgatgccgca aggaaaaggc tcgacagcct ggcgaaacct ccgggcagcc tcggcaggct
    10561 tgaggatata gctgtgcagc ttgcaggtat caccggacag acctgtaata ccctggacaa
    10621 gcgctgcgtt atcatcatgt gctcggacaa cggcgtggtt gaagaaggag ttgcgtccgc
    10681 gccccagtcc gttacgtacg cacaggccat aaatttcacc cgcggcatta caggcgtggc
    10741 cgtgctggca aaatgtttca actccgacct tatggtcgtc gatgtgggca taaacgcgga
    10801 agtgaaccat ccgggcatta taaacaggaa gatcaggaag tccacgtcaa atcttgcgaa
    10861 tcagccggca atgagccgcg gcgaagcggt aaccgcgatc ctcacaggta tcgaaacggc
    10921 aagggaagcc cacaggaaag gctacaggat gatcggcgtc ggtgaaatgg gcattggcaa
    10981 tacgaccaca agcagtgccg tcctgtgtgc gcttacaggt atcgatgccg gtctggcggt
    11041 cggaaaagga gccgggctta ccagcgaggg atttgagaag aaaaagcgga tcatcatgga
    11101 cgccataaag acatacaagc ccgatccgaa tgaccctatc gacgtgattt cgaaagtcgg
    11161 gggattcgac atagccgcca tggcgggagt atatatcgga gcggcttact gcaggctgcc
    11221 ggtggtcata gacgggttca tttcggctgt ggctgccctt gcagcagcaa ggatggccag
    11281 cctctccgtt ggatacatga taccctccca tgcgtcatgc gagccgggtt atgcatacac
    11341 cgtcagggag cttggactcg agcccgtgct tgacctggga atgcgcctcg gcgaaggaag
    11401 cggctgcccg ataatgttta tggtaatcga tgccgcgtgc gccgtgatcc gtgatatggg
    11461 cacgtttgcc gaggccgtga tcgacggcag gtacctggaa aacatcaggg aagaaggatg
    11521 cttttcaaca gacggcagga aaggggcgga atgttgagga tctttatttc gggcggctgc
    11581 aaaaacggga aatcctatat cgcccagaga cttgccaaag ggcaggcaaa agggcgcctg
    11641 tattacgtgg cgaccatgcg cccctgcgac ggggaggatg ccgaaagagt cgaacggcac
    11701 aggcaggaac gcgacgggtg ggggttcgaa acggtcgaac agccgatcga catactgaaa
    11761 tgcctcgact catgcgatgc ggatgcttcc ttcctgatcg acagcacgac ggctttgctt
    11821 gcaaacgaaa tgttcccggc cggcaacagc gtaaacatgg atgcgccgcg caagatcgcc
    11881 ggagaactgg cggggctgtg caggcaggtc aaggacatcg tcatcgtttc agattatatt
    11941 tactctgacg caatgctgta tgatgaactg accgaagcct accgcatggg gctcgcttat
    12001 atagacagga cgctggcggc tgagtgcgac gtggtgctgg aggtcgcgtt cggctgcatt
    12061 acggtgcata aaggcaggga ggtgttcaga cctttatatg aaaaggctgt gtagaggatt
    12121 ttttatgtcc ctgggcatgt tctgcgcgat accgttacct tatacaaaat gggaagaaaa
    12181 ctgtgcgaac ctggtcatcc cgtttttccc tgccgtcgga ctgatcatcg gagcgctgtg
    12241 gtatggagcc gcgcggctcc tgcttttggc aggactgggc aaggtgatgc tttcggcatt
    12301 cctgatgatt tttccctatg ccataacggg atttctgcat atcgacgggt ttatggacgc
    12361 cagcgacgcg atcctgtcac gccgttcccg ggaggagaag ctcaggatac tgaaggatcc
    12421 ccatgcggga gcgtttgctg tcgtttccgt cctggtcctg tttgtgatat gctttgcaag
    12481 cacatatgag atcgtttccg gcacctctgc tgccggaaaa ctgcgttttc ttctgttcat
    12541 accggcgata tcgagaagtc ttgccggggc agcgctgctt tcgttgaaag ctctgccgca
    12601 gagcagttat ggttcgtatt ttaagaaaaa tgttaagaca gtccacaaaa tagtcgttgt
    12661 tctgcccggg attgttgcgg cagcggctgt atgcctgctg ctcgggatca aagggtcgat
    12721 cgctgtgggt gcaatggctg ccggtttttc catctccatc attcatgctt accggcagtt
    12781 aggcggtgtt tcgggcgatt tgtcaggtta tgcccttacg gtttccgaag catgcggcct
    12841 tgtcgcgctt gcgctggtat aggggggttg gcgatggtac tggtctttgg cggagcttac
    12901 cagggaaagc tggaatacgt gatccgaaat tacgggatac aggaaaatga gatttttcac
    12961 tgcgatgacg ccagtgagat cgatttttcc aaaagggccg tatatggcct tgagaagctg
    13021 gtacttaacc ttataagatg cggagccgac ccgctggatt atataaatat gaatcttgat
    13081 aaaatcaatg ataaaataat tatctgtacg gacatctcgg ccggagtcgt tccaacggac
    13141 cctgtcatga ggcagtggag ggaagcggtc ggacgctgca tgacactgct ttcgcgcaat
    13201 tccgaaaggg ttgtccggat attttgcggt ttagggacgg taataaaatg aatgcgctta
    13261 tccatatcat acgccacggg cggacatttg cgaatacgaa tggcctttac tgcggcagta
    13321 cggatgtttc gctgtcggaa accggtatca gggagatact ggaatataaa gaaaaaggga
    13381 tctatcccga ggcggaactg ttcataacga gcggcctgaa gaggactgcc gagactctca
    13441 ggctgatcta tggcgatgtt gactatatta ccgtggcggg cctgcgggaa tataacttcg
    13501 gcgaatttga gatgcacagc cattatgaac tggaacacag gaatgaatac caggcatgga
    13561 taactgatga agccggcgat gtatgctgcc cttgcgggga gagcaggatc ggtttctgca
    13621 aaagggtcga acaggggata aacgatgtac tggatgaaat ggaaagacgc agggccggtt
    13681 ctgcagcggt catatgccat ggaggcgtca taatggccat catgcacatt ctgtttcctg
    13741 gtgagaggaa ctattatgaa tggcagcccg acaacgggct cggctatacg ctgcagtatt
    13801 ctgacggcaa cttttcagga taccggagca tagatccatg taaataacga agcatggatc
    13861 catgcagaga ataagtccgg gcacggagat aaacgcttat atgccctatt tggcttgtat
    13921 gcgtaaccat cgatgtaaag gaccgggcgg cgtccggatc ccgatccgtc atctttggca
    13981 gtacaggtga caaaaagtga caatataaac cggtaccagg cagtgagacg ggggatagtt
    14041 ctattctatg agttctgcgg atccggacaa gaaaccgtcc atccgccaaa aacagagaaa
    14101 agcctgattc atcaggcttt tctctgtttt tggcggagag tcagggattc gaaccctgga
    14161 tacccttacg ggtatacatg atttccagtc atgcgccctc gaccatctag gcgaactctc
    14221 caggtcgaaa aatatttacg tatggaattt cagcgccggc acttccagca tgcgctctga
    14281 agaggtccgg ccaattccgg ggatggccga atgctcgtgc aaagtgcttt gcgtgccgct
    14341 taactataat acaatagatc tggactgatg tcaatcggga ataaggcggt ggaacaatgt
    14401 ggacaaagct gtttgcggta tatctccagt ttcattgacg catatttgct acagtgatat
    14461 aattgataat aatgattgga ggagtcggcg atgaaatcga gatccgcatt cagtaacctt
    14521 aggatcacta atgtaaccag aaagaggccc gtcaggagga ttatctcaat aatcctgatc
    14581 ttattggcag taatcgttat cgccggcttt gcagccatac cgccattggt tatgaaatac
    14641 ttgatccttc tgcacgcgga tgtaagtgag atgccgccgg agcaattcgg cgtggaggcg
    14701 gaagaagtat ggctgaccac tgaagacggt ttccggcttg tttcatggga ggtcaaagca
    14761 agcaaaccca gggggatcgt aatattcctc tccggtatcc ataatccgtc agtcacggct
    14821 tatttcggcc atgccaaaat gatggcggac aacggatatt catcgctcct gatcgaaatg
    14881 cgctcccacg ggagaagcga cggggacaaa atatacgcgg ggaccaggga ataccttgat
    14941 gtgaaggcag gagtcgaata tatcaaaacc cggcccgaat ataacgatct gccggtaata
    15001 gtcttcggag tatcgatggg cggcgccacg gcgatcaatt cggccggaga gatcgccgat
    15061 atagacgggc tgatcagtat ttcggcgttt tcatcatggg aagacgcgtt tgccgacaat
    15121 atggccaaca tggggttccc gccgataatt gtaaaaatgg aaaaaccttt cgtcaaactg
    15181 tacatgtgct tcgtatatgg ttttggaagc attaagataa gccctgtcaa tgaaataaag
    15241 aagctaaacg gcaggcctgc tctgctgatg cattctactg aagattcgca ggttccctac
    15301 gacagtttcg aaaggcttaa ggaagcggct cccgaacaag tagaaaccta tgtccgcacc
    15361 ggcgattatc atatgatgtg tatggaagaa tatttcaggg aaccatggaa agacgaagaa
    15421 tatgccggcg ccatcctttc gttcctgaag aaatatttct gagtatgagg gttcttgatc
    15481 ctcatttggt tccttcatct caaaaatttc gctatgcctg agaaaagcct gacgatccag
    15541 tcgggcagtt ccgggaatgc agcacggata agagcggccg cctcagcgca tgtttgaggg
    15601 gtcagccggc ttatcttcag catataatcg ccgtctattt cattacctat ggcaacagtt
    15661 ccggaagcga ctcctcctat tgctatttta ccaagggcga ctcctccgaa agctccgagg
    15721 ccgactgcaa cacctccgaa tgcggcggta ccggcagcca cgccgcccag ggcaagcagc
    15781 agaccgaaag caaggccgcc aaacgcgatg acacccaagg ccaggccgcc gatacttatt
    15841 attcctattg gaatggcgcc aatggatact acgcctaccg gtagcgtgct gatcgagatg
    15901 ataccttttg accgcgtagg ccttccgtac ctgtttattt tgacgtggac cagcggtatc
    15961 ccgaaaacag tggcctgtga tttatattca tatatcggtt ttaagtattt cgcattctct
    16021 gaaatatctg acagcagttc atcgaagaac tcgcttttgt tttcatagat cgaatccatg
    16081 aattctcggg caacctgctt cggatctccc attctggcaa tcacgctgtc tatgtcttcc
    16141 gtgcgcgcag cttcattaag ctgtgaggtc aggtcttccc ttatacgctc cttcattttg
    16201 ctgtcggcaa tgatatagga cagaacttcc ttaacataac gatttatttt gctcattaag
    16261 gcattaccct ctttcatata aaaatcatgc ttagttccag tattcgttca gtttgcggtg
    16321 ccgcatggcc atatgttgtc agcctcatcc aaagggagac tgcaggaaaa agccatggtt
    16381 ttccgcattg cctgccatcc ttcagcgcag taccagccgt atcgcataaa taagcgtctc
    16441 gagttcctgc ttcatgtttt ccagcgcttt aatgccttca tcagttatca tgtagtactt
    16501 tctcgggacg cttcgtcttt cgtggtcctg ttcccagcag ctatcgatat acttgctgtc
    16561 ctcaagcctg tacaggacag ggtagagcgt gccttctttc attttgaaaa caccgcagct
    16621 tcttgcgtcc agttcctgta tcaactggta gccgtacatt ttctcttctc tcagaaggct
    16681 gagtatcagt atttctatga cacccttttt caattgtttg ctgacttctg tcaagtattt
    16741 gatctccatt cctaagtatt tagtaatact aaatatatga taataaaaaa tatttaccat
    16801 gtcaataccg attataaagg gaatgtactg attatgcgga gaaacacaag gatattgtgg
    16861 cgaacctgct gcctgtctga tagaattgat ttatgggaat gtgccttaag tccggacacc
    16921 atatctgatc cgggataaat atcaaatcag accatgtgga taaaataccg ggtccggagg
    16981 ataaatatga acagcatcta tttttatgac acacctgtcg gaagagcagc gatagcggat
    17041 aacgggagag ccgttatcca gctgaagttt atcagggaag gctggatcag cggggacagg
    17101 ctcggcagga gcgaaaccgg cgcggcagat gtttggccgg ccggaagttg cggaacgcca
    17161 tttattatat gcgaaacgcc gctgataaag gaagcggcaa ggcagcttta tgaatacctt
    17221 gacggaaacc gtaaagtgtt tgacatcccg cttgaactgg aagggacgcc gtttcagaag
    17281 gctgtctggc aggcgctcct tgagatccct tatggcgaga cgagaagcta cggcgagata
    17341 gctgaacgca tcgggaatcc gaaggcagca agggcggtcg gaatggcaaa caacaggaac
    17401 cacgtcgccg tattcgtgcc atgtcaccgt gtgataggcg cagatggcag gctggtggga
    17461 tatgcgagcg gtattgacat aaagaaaaag ctgctcgacc tggaaaagct gtatacaaca
    17521 aagaagccgt ctctgtaacc ggttgccggt tatatacggt aatgcagtta ccgggacagg
    17581 ataccggaaa acatgaaaag aaatatttga agaataactg tccgggtata cgatatgaga
    17641 tacaatagtc caagcaaacg gtaatcaaat ttaaacggca atcaaacacc gggcagacaa
    17701 ctgtcatgcg ctgacatatt caggaggcat atctgcaaaa tgaacgtacc ggcatatgat
    17761 gaaaagaata aaacaggcgc cagtcttcat ataggctgcc acctttcatc ctcggaaggg
    17821 tatctgcgga tgggcatgac ggcgctgtcc atcggcgccg atacattcca gtttttcacc
    17881 cggaacccga gaggagggaa ggcaaaggga atcgatcccg atgatgtcgc agcgttcctg
    17941 aagctggctg aagaaaatgg tttcgtgcaa ataatggccc atgcgccata cacgcttaac
    18001 ccatgctcgg ctgacaagcg ggtcagggca ttcgcgcttg aggtaatgtc ggatgacctg
    18061 gccaggatgg aatatatacc cggcagcttc tacaatttcc atcccggcag ccatgtgggg
    18121 caggggatcg aagccggcat tgcactgact gccgacctgc tcaacaggat actgaagcct
    18181 gatcagacaa cgacagtact gcttgagacc atgtccggga agggcagcga gataggaggc
    18241 aggttcggcg aactgcggca gatcatagac aaagtggaac tgaaggaaaa actcggtgtt
    18301 tgcttcgata cctgccacgt atatgacgca ggctatgaca tagtgaacga tctggacggc
    18361 gtgctggagg agtttgacga aatcatcgga cttgaaaggc tccgcgccgt ccatatgaat
    18421 gacagcaaaa atccttttta cagccgtaaa gaccgccatg cgaaaatagg cgaagggagc
    18481 ctcggcatcg aaacgtttgc aaacctggta aatcacccga aactccggca tttgccgttt
    18541 tatctcgaga cgcccaacga actggagggt tatgcgcagg aaatcaggct gctgaaggaa
    18601 atgcgccata tcattccttt accgggaatg cccgaccatg attcgatgtg caacggagag
    18661 taatatgcag gaaatgaaag atttacaagg gaaaatcaaa gcaagactta aggaactttc
    18721 cgaggggaaa tacagggagt ttgcctccgg cctcctgccc gacgtcaaca atatgcttgg
    18781 cgtacggctg ccggtcctgc gttcggttgc ccgggaaata gcgaaggatg actggcgggg
    18841 atacctggaa acggcagatg atgagttctt cgaagagata atgctccagg gcttcattat
    18901 cggacttgcc aaaactgacg tcgaagagaa acttcggcta atcagggagt ttgtccccaa
    18961 aatagggaac tggtcggtgt gtgacagctt ctgtgcaagt ttgaaattcg tcaatagcaa
    19021 taaggaacgc gtgtgggact tcctgcagca gtatttcaac tccgaaaggg aattcgaact
    19081 gcgcttcgcc attgtgatgg gtatctttta ttatacagat gatgattata tggacgaatt
    19141 tctgagattg ctggcccgga tcaggcacga cggttattat gtaaaaatgg ccatggcatg
    19201 ggctttgtct gtatgttttg tgaagcggcc cgggaagacc ttggaatacc tcaaaaacag
    19261 cgaatttgac gatttcacgt acaacaaggc gcttcagaag attatcgaat caaaaatggt
    19321 cgacgatgaa aacagggcaa tgatccgggg aatgaaacgc aaaactgttg actgaattca
    19381 ttcactcatg ttattataat attggtatta cctggcagca tcttaaacgg tggtggataa
    19441 attgacacgt atttcaaaag atccggaaga aagaagagaa gagatcatag agaccgcgca
    19501 aaagcttttc gaggagaaag gctatgaaca tacgatgatg tcggacatat cgcagacgct
    19561 gggtatatcg gcaggccttc cgtacaggta tttcagatcg aaactcgatc tgctggatgc
    19621 tgtcgcggcc aggcttggca aggagtttgt aaggagtctt atcggataca ggttcaaacc
    19681 cgggatgaat gcaaaaggaa aactggatga atatttcagg atcattgaag atatgggcgc
    19741 aacgaaactg gtttcgttgc tgcacagaaa ggacaacagc gaaatacaca aaaggatatc
    19801 ggaggcttct ttcagaagcc tgatgccgca gttgatcgaa ctgatcaggg aaggcaacag
    19861 ccagaaagtg ttcgactgtc cgtatccgga agagaccgca gcatttctta tgtatggagc
    19921 aatgtcggtt catgatatgg ttaataccga aaacatgcat gagaagatgg agatcattag
    19981 ggagttgttt tacagggtgc tcggggttaa gtaaaatgtc aattgacgga tatctccctg
    20041 ggcaagttgc ccgcgtattg accacggcag cagggtgatc tcctgacgat gacagaatac
    20101 aggaaatcgg agctgacagg taagtaaaat aatgtagaat acagacgacg gggtatatgc
    20161 ggaatgctcc tgcctgggag cttattttaa gcagatagag tgaatgagtt cattcaacaa
    20221 aacaatgagt atgattttgc tgtaaaaaca gtatttcaat aaaaggaggt catgttttga
    20281 gctatattac attcgaaaat gtcacaaaag agtacagaag cggcgaaaac gccgtattgg
    20341 cagcggataa tgcgaatttt gcgatagaaa aaggagagct ctgtgtcatc ctcggaccca
    20401 gcggcgcagg aaagacaacc gtgctgaacc tgctgggcgg catggacagg gcgacttccg
    20461 ggcggataca ggtaggcggc agggaaatca cttcctttaa tgaagaggaa ctgaccgcat
    20521 acaggagaga agctgtcggg ttcgtgttcc agttttacaa cctgatgccc aatctgacag
    20581 cgctggaaaa cgtagagctg gcaaagcaga tatgtaaaaa ccccctggat cctgtcgaag
    20641 tgcttgagca ggtaggcctt ggggacagga tggacaattt tccttcgcaa ctgtccggag
    20701 gggaacagca gcgtgtttcg atcgcaaggg cgctgtgcaa gaacccggca ttgctcttat
    20761 gcgacgaacc tacgggcgca ctggactcca ggaccggcat gcaggtcctg aagctgcttt
    20821 cggatgtctc cgcaaaatat gggacaacag tggtcatcat aactcacaac acattgatag
    20881 cgccaatagc gggcagagtg atccggatga agaacggccg tatagaatca gtggagaaaa
    20941 ataatgctcc ggtgccggtg gaggagatag catggtaaag aacaggcttt ttacaaaatt
    21001 cctgagagat atgcgccatt cgaaagggca gctgttgtcc gtcatgttgc ttagcatgct
    21061 gggcgtatgg gtattcagcg ggctggatat atactggcgc aacctggatg tttccattga
    21121 gagctatttc gatgagcaga aactggctga tttctgggta ataacaccgc tggctgataa
    21181 ggatccggag gaaaaaataa ggagcgtaga cggcgtcgaa gatctgatga gcaggctgag
    21241 ttatgaggtc agtacggccg tgcctgagga tgcaaaactt gtgctccatg cagtcggcga
    21301 cgatatcgat attaacatcc ccaggattac gagcggcggt cccctttggg acagtgacag
    21361 cgacggatgc ctccttgatg tgcggtttgc cgaagccaac ggccttgccc atggcgacag
    21421 catagtgctg aagataggag aggagaaacg cgagttcgtt atacgggggc tggtaatgag
    21481 cccggagcat gtgtacattg cgccggacgt tataccggag ccgaagatat acggttttgc
    21541 ttatgtgaat aaagcagcat ttccgaatat accgtcaaat gaaatatgtg tactgctgtc
    21601 cgacgatgcc gatactgagg aagcaaaagc cgccattgaa gataagctgc cgggagcatt
    21661 catacaggac agaagatccc acaaaagcac acaactgatc agtactgagg tctcgcagtt
    21721 cagatccctc tcgaaagttt tcccggtgat gttttttgca gtgtccgcac tgatcgtgat
    21781 gacagccatg accaggatcg tctctaacca gaggacacag ttggggctgc tgaaggctct
    21841 tggatacggt aaaggtacga tagtcctgca ttacatgtct ttcggcttct tcccttccct
    21901 gggcggggcc gcgggcgggc tggcgctcgg ctgcaatacc ctgccggcgt ttctttggaa
    21961 catactggat gatatctata tactgccgga cgtaaagacc gcgccgcttt cggcggctac
    22021 attgacagtc tgcatgtcgt cggtactgct gacatgcatt atttgctggt gtgcctgcag
    22081 gcgttcggca gccgaaactt ctgcttcgct tcttagaccg aaggcgcctg cgtcaggcag
    22141 caggatactt cttgagagga tatcgccgat atgggagcgc ctcagcttca gtgcgaaaat
    22201 gatccagagg aaccttttga gaaacaaaat acgcaccata atggcactgt cgggtgtcct
    22261 cagctgcacg gcgctgataa tcacggccct cgggctgctg gattccgtgg accacatggt
    22321 cgatacctat tatggcgaaa cactgaagtt cgatctccgt gcggaccttg atgaaggcgc
    22381 gggaagcgcc ggcgagtacc gcaggctgat cgatgcggga aaagtcgaag gaattatgga
    22441 aatggcagca agtataagga agactgacgg gaacggcagc cgtgcagtgc tgcttactgt
    22501 tgtcgagaac gaccatgaac ttctatcact tgacacagca ggaacatcgc ctggcacgga
    22561 agaaaccggc aagaatggcc ggaacacgcg gcaatcatat ccggccgaca gttatgctga
    22621 tgataaatac cgaatcggta tacaagataa cagggtacag cacgacatgc cggataccgg
    22681 agcagaatat gaaacagaaa tccggcatcg ggaaagtgtc attgcttcgg aaaagcttgc
    22741 acaggtaatg ggaatcagcg cgggtgatat cttaaaactc aagctcccgg gcgcggcgga
    22801 ggaggtggaa gtatttgtcg aacgccttgt gccggtaccg atcggtcagg ggatctatat
    22861 gtacgaaagg tcatggaaca gcctcaaaaa aggtgaattt acccctaccg cgctccttat
    22921 aagaaatccg tgcaaagagg tttatgacct tctggaagga ctcgatgagg tgacatgcat
    22981 taagtctctg caaacgctca gggacaagac aaaagagggg ctggaatcct taaccggcgt
    23041 ttcagtgctg atggtcgctt ttgcattggt gcttgctttt gtagtccttt ataacatggg
    23101 catactgaat ttcatggaaa gggtccgtga atttgcgaca ctgaaggtcc ttggtttccg
    23161 ccacagggag ataaggtcgc tgatagtaaa ggaaaatatc atcatcgctg tgacaggcat
    23221 cctggctggg atttaccctg gcgtccggct gacagatatc gtgatgaaat ccagcgaacc
    23281 ggaagacatg gtattcagcg cagtggcaga accgacgagc atactggcgg catgctgcat
    23341 cacgttcgtt ttctcgctgc tggtccagta catgctggcc gggaaagtca ggagcatcgt
    23401 gatgacagag gcacttaaaa ttgttgagtg atcttgtatc cgatttgaaa ataatgcgat
    23461 tttttgatat atttatattg tcacacaact tcatattctg tcgattttat atatctcagc
    23521 cgctataacg cgaccggaaa gtttcaaccg ttttcaacgt taattatcgg aataacatgc
    23581 tgctgacagc aacatgctgt tcagcggctt attatgcatg gacaatatga aatcgtccat
    23641 atttcaataa ataaaaaacg ggaggagaga taatatggtc gcaactgacg aaagagcaac
    23701 gttgaaagtg tggaggagag atgttgtaaa cagcaggtcc agcagaaagc tgaggaaagc
    23761 cggttttgtc cctgcttcga tcagcatcag ggggaaggat tcgatttcgg ttaagataaa
    23821 aacggacgag ttgatgaaaa acctgaggaa atatggcagg aaccacctgt tcaatctcga
    23881 catcgacgga aatgaaacgt acaccgcgat agttaagggt atgcagtatt cgtccgtcag
    23941 cagggaactc atggatgtca gtttccagca tgtatccctg gatgaggaga taaaggtcga
    24001 agtcgaaatc aaagtaacag gaagggaagc tgcagaatcc aggagactgc tggttgtcac
    24061 gcagctggac aaaatacctg taaagggcct gccgcagaat attcccgaat acctggagat
    24121 cgatgtggca aagctcaatg cggatgacaa gataacagtg ggcgatgtca ggtatccgga
    24181 aggcatagta ccggactgtg atactgaaaa aatagtgctg gtgatcacgg aagccaagtc
    24241 cggcgctgca gcagagagcc aggagggcca ggaggaacag gaggatcaga aagcggccga
    24301 ataacgggat atatgggatc ccatggcccg cgatcgtaag atttataact ccgtctcatg
    24361 gatatttgcg tttcgtgacc agagacgaaa aaaaggggct ttgatgccgt taggataata
    24421 tcggcatgga gtcccctttt tactgaaagc tgttggaaat atcatgtttt gactctatgg
    24481 tcttattttt ctccggctta agccgtcata agtggtttca tgctgttgct ccggttccgg
    24541 atcaggaagt ggctttatac gctttacata gaaatatctt aaaaatgcaa tgcacatcgc
    24601 gactgaaacg atctcggcca gcggatatgc ccaccagacc gcatccagcc cgcctgtttt
    24661 cgcgaagacg tatgccagcg gcaaaagcgc cagaagctgg cgtgccaacg attccagcat
    24721 actcggcaga ccgctgccca gcgcctggaa tacagagcct gacactacgc agaaaccggc
    24781 gaatatgaag ctgatgctta ttatcctgaa ggcagggacg ccgatctcaa gcatcgtttc
    24841 cgaggctttg aacatcgaca gtaaatggcc ggggaaggcc tggaacaacg caaaacctat
    24901 ggccatcacg acggtcgcat aaaggatgct tagccttatt gcctgtaata tcctgcgttt
    24961 gcttcccgct ccgtaattgt acgatatgat cgagactatg ctggtgctga gcccgaatac
    25021 cggcatgaaa aggaagtgct caagcctgga gtaagcgccg aagactgcga tcgcagtggg
    25081 ggtgaaggcg atcagtatcc tgttgagacc gtatatcatc accgaactta tcgacatcat
    25141 tacggcgacc gggatcccga cagcaaagat cctttttatt atttcggcac gtggcctgat
    25201 ttctgcgatt ttcagcttta tttcgctgtt cagggaaatg ttcagtacaa aggccagcgc
    25261 cgcagccgtg cactggctga tgaccgtggc gacggcggct ccctttatcc ccattgccgg
    25321 cagcccaagc cagccgaaga tgagtatcgg gtccaggatg atgttgagaa ctgctccgca
    25381 acactgcgtg atcattgaca aatgtgtttt tcccgtcgac tggagcagcc tttcaaatgt
    25441 gacctgtccg gcgagcccga acgaaaatat cgtgactatg ctcaggtatt cagtaccata
    25501 gtcgattatt tcctgaaatt cggtctgtga tgtgaagtaa taccgggtga acagaattcc
    25561 cagtaccgca aacaggatac tgcccgcaat acccaggaat atgcccgtat ttgcagcctg
    25621 gctcgccctc tcccggtttt tttcgcccag gctctttgag agcagggcat tgacgcctac
    25681 accggtaccc gtcattaccg ctatcatcag atattgtgcc ggataggcca tggacagagc
    25741 tgtaagcgca ttttcattga tacgggatac atatacgctg tctatgaaat tatacagggc
    25801 ctgtaccagc atggaaagca ttaccggcaa cgacatcgtt atgatgagcc tgccgacagg
    25861 catcgcgccc atcttgtttt tgtccgtggc cctgttttcg ttgatttccg cagtggcttg
    25921 cgtcatttct tccgcccccc ggatgattat ggaagaaaaa ctaattaata taaaaacctg
    25981 attatattat atagcatctt tattgccctg caaagggaag atgccgaaat caacgacgca
    26041 tgtaaagctg cggaaggaga tattatgcat gtcttcctgc cggagccggg gaaacggata
    26101 cattctgatt cagccagtcg gtaaactgtt catacgtgat agcgcctttc cgggttttct
    26161 ccaactcctg ctgggcggca taagaccatt gccgcagctg cttttttgct tctttctttt
    26221 tgcctggatc cgatctgcat ggtttgaccg ttcttgcata catggattga tatgccttga
    26281 tgtaggctgc cagggccgga tctgtcctta ctttttcctg gaaggttttt atggcgccaa
    26341 tatccttgca ggtttttccg ttgtcagtca cgaccctgtc gcaatatgcc gtatcgatcc
    26401 tgttcgaagg gaggaaatac tttttgcagt tgctgcattt tttgatcatg atattgcccg
    26461 ataccatttt gaaaaattcg atataacata tcccgtaaac gtctttaata tcccatactt
    26521 ccgatatcac actgtctccg cccgaatcgt cgatctcata agtcatgccg ggaactgaga
    26581 cctgctcgat ttcggtttgc gaaaatatgt tggagcctgt ttcgaactgg ttcgcttttg
    26641 atatgctgat cctgtcgacg ccgtattcat aaaggcagtc tatcggcgtt ttgccctggc
    26701 ctgcattttc tatgtccagg caatgatgcg ccgcttttgc gaaaatattc tggacttttt
    26761 ttgcagtatc cagcaacttg ctcgcactca ggcatacatt attgaaaacg agctttacct
    26821 ttttcttctc tccatcgtcc agttcgcgcg agtcggatct gtttatgtga acgaatgatt
    26881 ccctgagcct cctgtcgatc tccgacttaa ggtaatcggc atatgcgaaa aaatacggat
    26941 ggattttaca caaagaggat aggaagatct catagtattt cctgactatg ttcctgtccc
    27001 aggatgattc agattcgata ctttcgatat agccggtcct gaagagctcc gccgcctgga
    27061 cgaagtcaat attgatgaac tcggcaaagg cgctcccgat gggcatcgac acattatcaa
    27121 tgataagcgt cctgttgttc cggaaaacga aacgcatact tcctccacct cttcttttgt
    27181 attttgaaaa ctttcgtaac gggtaaatta tcaataattc attgcaataa ttatatattt
    27241 catggctgat tttgtcaaat aatgtattgc gaaaataaat aagaggcgta ctcagcaatc
    27301 gatattggag ccaggcaatc atgaattacg gatgtgcggc cggaaatgca ataaaaatac
    27361 cccaggctaa atagcctgag gctgttggtg cgcccgaagg gattcgaacc cctggcctgc
    27421 ggtttaggaa accgtcgctc tatccttctg agctacgggc gcgtctttca ctgcccttag
    27481 tgaagggcca tcattcacag aacgttataa ttttataatt attatagcgt attgtcaagc
    27541 attatgcgag ttttatgagg caatgggcaa aatttccggg caaagtctgc ggaccgcgcc
    27601 attgcggaat ccggccgcgc cgatatcaaa tgttaattcc gggcctgttt atgccgatat
    27661 ttttactgga atatgctttt ggaggaatgt tatgatttac ggcagatata ccggaaaacc
    27721 ccgggaaaaa gaacaggacc ctctaaatat caacaaaact gcgaggaata atttcataag
    27781 gactttttta ttccttttta tcataatggt cgtgttcatg gccatatcgc attatgccga
    27841 tgctttcgac cctggtttca atgcgtatcc cctcagaccc gataccgaca cgaagatgca
    27901 gctggagtgg ctttctgtcc ccaatgccga attttacaga attttcaggt atgatgaaag
    27961 cataaatgat tttacactta tcaagtcaat aaacgtaggc gggacattgg atccgttctc
    28021 atataccgac cagggactga aaccggaaac gacttacaga tacatgcttg ttgcatatga
    28081 tgcaaacagg aaagtaatcc tcagtgacga agctacgaac acgactacgg cgatgatacg
    28141 cccgcatggt ttaagcgctg tgttcgatgt tacctcaaga aaggcattgc ttacatggaa
    28201 gagttccgca cttgctgaag gaagcatgat aaacatgacc gaaaacggtg tttcagtgcc
    28261 tccatttccc ttggatatac cggaaacaac ttcatatact gtagagatac tgggttcgaa
    28321 ccctgtccaa tttactgtcc ggacaaggtc gggggcgtat ggtacgtctg cgctatccga
    28381 tcctgtgacc gttgttcccg tggcactgcc atacctgact gccgaatatg tgaatgaaag
    28441 caccgtaaag atctcgtggg gcagttcgac aaatatagac gtttacgtgc tggaacgttc
    28501 aaaatgggac ggcgcttcct ggggcacatg ggagaccgtc agcactacat tatccggcac
    28561 ttatatgaca caaacggtcg aagagggagg acagtacaga tacaggctga gagcaaggga
    28621 agataaaggt tacaccggca acagcaacgt aacgaattac gtcaacaacc ttgtcgcacc
    28681 taaagacctg aaggggaaaa taaccgcaga agaccagatc gatctttcat ggaccaatcc
    28741 gccagggaac acaggcagac tcgaggtttg gcgcaaagcc ggcggcagca gcacgtcggg
    28801 ccagtatacg cttgtgggga cactcaatac taatgacacc actttctccg acaaatt
//
LOCUS       DAOSCC010000005        28631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1209_length_28631_cov_4.276631, whole genome shotgun sequence.
ACCESSION   DAOSCC010000005 DAOSCC010000000
VERSION     DAOSCC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 28631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28631
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1209_length_28631_cov_4.276631"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..2206
                     /locus_tag="PLG72_00680"
     CDS             56..2206
                     /locus_tag="PLG72_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HOJ79349.1"
                     /translation="MLEGKNPGTPAAVKDIAPDDIVFAMDIGTRTIAGVIGRQEGDVF
                     HVLATEVCEHKSRAMMDGQIHDIDQAAATAMEVKRRLEYRIGFRLKRVAIAAAGRVLR
                     TCQVKVEKNLEQDMEIDADLVSSLEIEGIQRAQTVLDENSEAEDKNQYYCVGYSVINY
                     YLNGYVISKLTGHKGRTAGVELLATFLPLTVVDSLYSVVNRLGLEVASLTLEPIAAIN
                     VAIAPDLRLLNLALVDIGAGTSDIALTKDGSVFAYAMASVAGDEITESISQRYLVDFA
                     TAEKIKVSLSSGKEKIRFTDILKKKHEITAEEVLRDIGDTIRMLASAISGKILEYNRK
                     APNAVFLVGGGSRIPLLTKFLAEQLGLPEDRVVVRGRDVVRGVKYSDKKLYGPEAVTP
                     LGIAVTAQMYAGKDFLSVTVNDKKLKLFNSKKLKVSDALVLYGFNGGDLIGRSGKSIT
                     CTVNGEERRIRGEYGKAAEIWLNGKLSSLDASLSFGDNITVIPAQNGRDASVKAYELV
                     PDYCTGSVILNGSPVDISTIITVNGRQVGAETEIKDGDTVQFSRIRTLGDLLEAAGFT
                     GMDCNITVNGISDCRYDYVLKDRDVVICEERREDYSPAASEDHGGSVGNLHKNGAETE
                     AGIGTGTEAETGDMMNIYTRQEPDEWFDVTVNGKTVSLRKNQARHMFVDIFNYIDFDL
                     SKPQGTIVLRLNGRPAAFTDVIGEGDEIEIYWRK"
     gene            2390..3958
                     /locus_tag="PLG72_00685"
     CDS             2390..3958
                     /locus_tag="PLG72_00685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOJ79350.1"
                     /translation="MIWSAAACVTVFALLLIRYKVFRSFMLNNILVYCLEKVLFSSIV
                     SAIAIFSGIGVWFYIGYLLPMCATTYIKGKKYGYATAVTSAIIHMLMLYGRISLAEAG
                     GIASDVKKTIGMAAGLYVISFITVYFFGELYKNRIEEEFQATYATEQFNERYQMLKSE
                     MDEIIQKYSSLITNSDKLEESNKSLSKSIAEFYTLNQISQAIGSILDTRELLKRLNDI
                     ILGVVGGSYSTIVLYDEESGRLKVHTTNVTNPAELATLMDNINCSILMDVLMKGECLL
                     ENNVDYYQYLFTCGRNINSLMCIPLTTSSRKYGLILVEHTMSNVFDTENVRFLRIIAQ
                     QVGIVMENAELYLKMKEMARKDGLTGIYNRQYFQERLQAEFREAQKNGYPLSLVMYDI
                     DHFKKFNDMYGHLFGDKVLKSVAEVVASTLRKGDMLARFGGEEFTILLPQTSLDEAYE
                     KVETLRKLISQHVIEDNLINVSVTASFGISSYDECALNENDLVRTADYALYEAKASGR
                     NCVMTARRLYEVRH"
     gene            complement(4238..4426)
                     /gene="rpmB"
                     /locus_tag="PLG72_00690"
     CDS             complement(4238..4426)
                     /gene="rpmB"
                     /locus_tag="PLG72_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOJ79351.1"
                     /translation="MARCVICDKGTVFGNNVSHSVRRTNRAWKPNIKKVRVIENGTPK
                     TITVCSRCLRSNKVTRAV"
     gene            4805..6880
                     /gene="recG"
                     /locus_tag="PLG72_00695"
     CDS             4805..6880
                     /gene="recG"
                     /locus_tag="PLG72_00695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOJ79352.1"
                     /translation="MHKGSELLNKQLQYLKGVGEARAALFRRLGVHTVGDVISHYPRD
                     YEDRSRLKKIIQLQDGDQCSFEGIIASRVVVSKPRKGLAISKVSIRDDTGLINAVWFN
                     KPYLKDQLKPGERYLFFGKVTRRGTFEVLNPVYEKTDESGPVNTCRIIPIYPSTGKLT
                     QNVIRSVIRNALAYAGDSIEDILPGWIRERYHLAGAQYSISNIHFPESDEAFLKARER
                     LVFEELLILQLALMSVRTVQESAVPGITFGAREEVRRFVEGLPFRLTNAQLRVLDEIE
                     RDMESSKVMNRLVQGDVGSGKTSVAVAALVKAVKSGYQGAFMVPTEILAAQHYSSLKD
                     LLGPLGFNIALLTGSVGTKDAKRVQAGVSSGEIDIAVGTHALIEDKVVFNNLGLVITD
                     EQHRFGVRQRAALGKKGACPDMLVMTATPIPRTLALILYGDLDISIIDELPPGRKKVL
                     TYAVDSSMRERVYNFIRKQVAEGRQVYIVCPLVEESDAVDAVSASELAFQLAHKTFSD
                     HKVGLIHGKMRPSEKDDVMRSFISGETSILVSTTVIEVGVNVPNATLMVIENAERFGL
                     SQLHQLRGRVGRSSYQSYCILFNDSKSEIAKERMKVMTQTSDGFVISEKDLELRGPGD
                     FFGTRQHGIPDLKIANLYKDMEILKKAQEAAEMLIRDDRKLETRENLLLGQAVREKYS
                     GVINSISMN"
     gene            6917..7483
                     /gene="rsmD"
                     /locus_tag="PLG72_00700"
     CDS             6917..7483
                     /gene="rsmD"
                     /locus_tag="PLG72_00700"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOJ79353.1"
                     /translation="MLRVISGSAKGHNLKTLKGMATRPTSGMVKEALFSIIAPKVGDS
                     KVLDLFAGSGGLGIEALSRGAAWAVFIDRNPRCCSIIKENLAHTKLLDFAEVYAIDYS
                     DAIRKMRREQRKFDIIIMDPPYNKNFAQEALKLLTNNDIMKDDGIIIAEHSASDDLPE
                     TCGRFRAIVRRGYGDTMITIYKDGKDGL"
     gene            7480..7959
                     /gene="coaD"
                     /locus_tag="PLG72_00705"
     CDS             7480..7959
                     /gene="coaD"
                     /locus_tag="PLG72_00705"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ79354.1"
                     /translation="MTLVYPGSFDPITNGHLDIIIRAACICDELIVAVGNNESKKPAF
                     TVEERVEMIKESIGGRENILVTSFTGLLADFAKKVGARAVIKGLRAMSDFEYEFQMAL
                     LNKKLAPELETLFMMTSVQFSYLSSSAVKEIARNGGCIDGLVPDCIKERIVGKLTCR"
     gene            7980..8417
                     /locus_tag="PLG72_00710"
     CDS             7980..8417
                     /locus_tag="PLG72_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOJ79355.1"
                     /translation="MEILSILETLEDLVEKSVTVPFTGKCLVDKEEILEIVKELRLKL
                     PDDIKQAKWVKEERQRILLDAQKEANNMIKDAEGKIASMVNEHEITKKAYEQANEIVA
                     AAQKNAREIRLGTKEYADNILNKVEEILTDTLAVIKANREELK"
     gene            complement(8410..9537)
                     /locus_tag="PLG72_00715"
     CDS             complement(8410..9537)
                     /locus_tag="PLG72_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HOJ79356.1"
                     /translation="MSSSGFTYMAGTLLEPVMRPLFNLPGCSSLALALGVTSGYPVGA
                     KITADLRKNKAITKTEAERLLAFTNNSGPIFIIGAVGTGMLGSPAIGYFLFICHLLAS
                     LTVGLVFRFYRQSGKTPSVPGNIPVPRSAADLEGKRHSMGPFGTGSQNRKIARTDPGK
                     KLKMGENTWKNPGILLGDAVRNSVATILSIGGFIVLFSVVISFLEETGLIRLAVSAVL
                     RLMPEYMLKWDMNGVVSGVLNGLLEITTGTGTVSRCVNVPLHVKLPAVSFIIGWAGLS
                     VHLQVMGIISGTDINIRPYLLGKMLHGTAAAIYAWAGMGLLPPGSVAAQPTLGLPAFD
                     FHFFVRTIGHSSLILAVVIAVWIGIISLILLFKAMKKKSPI"
     gene            9512..9799
                     /locus_tag="PLG72_00720"
     CDS             9512..9799
                     /locus_tag="PLG72_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79357.1"
                     /translation="MYVKPDELNSSEAAKKGKSDGTTTAVQRFRPFSAAFAAVSEKRT
                     RHTVKMTHNAGKASRRRYMVMGCRTDISSATETRIRTRRVYIIAKLPLKCY"
     gene            complement(9951..11021)
                     /locus_tag="PLG72_00725"
     CDS             complement(9951..11021)
                     /locus_tag="PLG72_00725"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOJ79358.1"
                     /translation="MAQEIKIQKTQNPKPKPDQKNLGFGHYFTDHMFIMDYNPEKGWH
                     DPRIVPYGPLCLEPSTMVFHYGQAIFEGLKAYKSADGRIFLFRPDMNMKRINKSNDRM
                     SIPPIDEEFGVEAVKALVSIDKDWIPSEPGTSLYIRPFIISTDPYLGVRPSYTYKFII
                     ILSPSGAYYPTGINPVKIMVENEYVRAVRGGTGFAKTPGNYAASLKSQEIAYEKGYVQ
                     VLWLDGVERKYIEEVGSMNVFFKIGDEVVTPALDGSILPGITRDSSIHLLRKWGYKVN
                     ERKLAVQELFDAYAEGKLDEAFGTGTAAVISPIGELNWDGKVITINDGKIGEVSLKLY
                     NTITGIQNGTVKDEFGWITVVE"
     gene            11203..12717
                     /locus_tag="PLG72_00730"
     CDS             11203..12717
                     /locus_tag="PLG72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ79359.1"
                     /translation="MDNNVASRRNVDYRSASRRVNRFVLLLNWAIDIFLIAGYALEYL
                     KGGKKLSYLMVMSAIVIIPMVIATIIFRKNNRSNIMKYVTLSGYFILYTFVMFTASPG
                     RLQVFAYMFPIVLSYFLYFDLKLVLIGGTAFTLVNIARIIWLMFFLGQNSSFETTDYL
                     IQFGCVLTFSLSLIYSTKISNTFSDEKIMDIRTEQENQERILRDVLKIAAVLDRNSKE
                     VHRIVKELEEITNVASNAVQEIEKGAADTAANIQVQSGLTHDIHQLISDTSKDSETME
                     LISVNTAEAVSEGMKIIEILNGKSDDVSQNSDSAYNIMLDLKKKTGEIRTITDLISSI
                     SEQTNLLSLNAAIESARAGEAGKGFAVVADEIRKLAVQSKESTDSIIKIINDLYEQTD
                     RSVEAVLKLKEANEEQNALVSRTMEIFTSISRKMDEVRESVNRVNEKVSKILEANNRM
                     VESINEISAVSEQVTASAEEACALSAQNIEKAKEAGMYVEELIETSMQMSKYQN"
     gene            12754..13254
                     /locus_tag="PLG72_00735"
     CDS             12754..13254
                     /locus_tag="PLG72_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOJ79360.1"
                     /translation="MNEIFLPRMYVDSFAEITADILRKNDIQGLILDVDNTLVPNHVA
                     DPDDSVLNWIESMKASGFKLCIVSNGAKRRVSRFNEKLKLYAIHRAMKPVKSAFLKAG
                     RLMGLESRHVAVVGDQIFTDVYGGNRAGMFTILVRPIDQREGRLVKLKRIFEKRILRG
                     YAPKTK"
     gene            13279..14181
                     /gene="aroE"
                     /locus_tag="PLG72_00740"
     CDS             13279..14181
                     /gene="aroE"
                     /locus_tag="PLG72_00740"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ79361.1"
                     /translation="MVLDKRVDGRTKLTGLIGNPVEHTVSPVIHNSLFSYLGINGIYI
                     PMKTDEAGLETAVRGLAALGFTGFNVTIPYKEAVCDLLDETDDEVKLLGAANTVRIKD
                     GRLYGYNTDGEGFVTAFKKQTGSDFSGKKICILGAGGTARTLAVKVVQEGAGEVCIIN
                     RTVPKADEIAGYINRTLLKNSGLRKPVFTAAPGTGQAHGVLNGSEIIINTTSVGMFPN
                     TDASPVGDNVDYLTKPIVYDVIYNPAETRFLASARMKGCRTYNGAGMLFYQGIKAFEI
                     WMETKVPDDVLQELSTSFLKYLEV"
     gene            14239..15039
                     /locus_tag="PLG72_00745"
     CDS             14239..15039
                     /locus_tag="PLG72_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOJ79362.1"
                     /translation="MAAYYVFIFILGLVVGSFVNVLIYRVPLHKSIVAPPSSCTSCGE
                     RLTVPDLIPVFSYIFLKGRCRHCGERISPRYPLVELLTAAMFVILFTRFGFTVPFIAY
                     AYLMTILAAVFFIDIDHRIIPNGLVLAALAGGLAFFAWNCISPDPLVFGDEKWWTPLA
                     GLLPGSGFLLLVAILGSLIYRTEDAMGMGDVKLMAPVGLFLGWRLCITALAVSVLLGG
                     LVSMALILLGIKKRKDTVAFGPFIVAGTFVTMIWGWELTEWFFNYINA"
     gene            15084..15350
                     /locus_tag="PLG72_00750"
     CDS             15084..15350
                     /locus_tag="PLG72_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOJ79363.1"
                     /translation="MVQIKNYMEDVVFGQMKEVLADINMCTCEKCLLDVAAIALNDLP
                     PKYVVTEKGELYSKVNTLGQQFEVGVVAALTKAAVLVKRRPRHI"
     gene            15405..15842
                     /gene="aroQ"
                     /locus_tag="PLG72_00755"
     CDS             15405..15842
                     /gene="aroQ"
                     /locus_tag="PLG72_00755"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOJ79364.1"
                     /translation="MRKRIIVINGPNLNLLGTRETDVYGTVTLAEIAAAVVEEADSLG
                     LDVDFIQTNHEGEIIDKLHESRGKYDLVILNPGAYTHYSIAIRDAVKAVELPCIEVHL
                     SNVHAREEFRSKSVIAPVCVGQISGFGANSYIIALKAAALLLS"
     gene            15864..16940
                     /locus_tag="PLG72_00760"
     CDS             15864..16940
                     /locus_tag="PLG72_00760"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOJ79365.1"
                     /translation="MTIYGNRLDSIRRKMASSNIDAVLVTKRVNYMYLSGFTGTAAIL
                     FISGARAVLLTDFRYVEQAAKQAPDYEIVRYVTDQYEEINKLLESEGIKRLAFEECHL
                     SYKQYTQYLGKLKVDEFVPLGGMIEELRSIKDETEISLIRKAVDIADDAFSYILGVIK
                     PGITELDIADELEHHMKRLGAEGPSFETIVASGKRASMPHGVASEKKIEHGDVVTLDF
                     GAIYKGYCSDITRTVFVGKPEGEIEKIYRIVLDANRRGIEAVRSGILAKEADSAAREF
                     IKEAGYGDNFGHGLGHGVGLEIHEDPTLSPRGDIVLKDGMVVTVEPGIYVTDLGGVRI
                     EDMVVVNGDSALVLTKSPKEMIIL"
     gene            17088..17645
                     /gene="efp"
                     /locus_tag="PLG72_00765"
     CDS             17088..17645
                     /gene="efp"
                     /locus_tag="PLG72_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ79366.1"
                     /translation="MITAGDFRNGVTFELDGQVFQVVEFMHVKPGKGAAFVRTKLKNV
                     ITGATIERTFSPTDKMPEAHIERKDMQYLYNDGDLYYFMDMETYEQVPLGADTVGDSL
                     KFVKENMNVKILSFKGNVFGIEPPTFVELEVVETEPGFRGDTATNTTKPATVETGAIV
                     KVPLFVDKGDVIRIDTRTGEYLERA"
     gene            17749..18198
                     /locus_tag="PLG72_00770"
     CDS             17749..18198
                     /locus_tag="PLG72_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79367.1"
                     /translation="MNYLRERVAYLKGLADGMKLDTSTNEGKLLKAILEVLDDMALAV
                     DDIEEAQEQLSEQVDEIDEDLAEIEDLLYGVDDEDEEEYCYDEDDDEEDEEIVELECP
                     HCGETVNLEDAFMRGNTVLCPHCSKEIEIEFDCDCEDCEDNEEDAED"
     gene            18293..18895
                     /gene="lepB"
                     /locus_tag="PLG72_00775"
     CDS             18293..18895
                     /gene="lepB"
                     /locus_tag="PLG72_00775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ79368.1"
                     /translation="MSVKALLREIIEWIVIFGAAFVLVALLNTAVFSATQVKQTSMQD
                     TLQEGQHLLIEKISYTFGDPSRGDIIVFLENKYPENYIEKVRIFITDVGDILKPPERK
                     TNIRMVKRVIGVPGDEVDIRDGKVYVNGIELEEEYVKGVTFQREQPMPVKLDEDEYFV
                     MGDNREVSKDSRTFGPVDRSQIEGKAVFRFWPLSGFGTLK"
     gene            19074..20096
                     /gene="spoIIIAA"
                     /locus_tag="PLG72_00780"
     CDS             19074..20096
                     /gene="spoIIIAA"
                     /locus_tag="PLG72_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOJ79369.1"
                     /translation="MKENSDGKLSVKAMSSLRKDILPMISGRVAAAISGALIYGLEPL
                     EEIRLRAEKPLVLQNWSREWFIGSDGLPSPKPAGSFIVTQEDIRTTLELMSENSVYAY
                     QDEIKNGFITIRGGHRVGIAGRAVLDGESIRNIKDISGLNIRISNQVFGCSSKVLKYL
                     IKNGTDIYNTLIISPPQCGKTTILRDIARNISNGVPELGFKGVKVAIVDERSEIAAML
                     RGVPQNDVGLRTDVLDGCPKSIGMKMMIRSMSPKVIITDEIGNDGDREAVHSVLNAGV
                     RIITTAHGYNISELKSRREVLMLLEDKVFERYIVLDDSDGPGTLSEVVDGTDMRVIWG
                     SGHHVS"
     gene            20086..20604
                     /gene="spoIIIAB"
                     /locus_tag="PLG72_00785"
     CDS             20086..20604
                     /gene="spoIIIAB"
                     /locus_tag="PLG72_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HOJ79370.1"
                     /translation="MFLKVLGSIIVLLSSSFLGYIMSRDCSKRPQQLRSLQNLLQMFE
                     NQISYSSDVLAEAFERISRAGGETGIFFRTTVDLLKNGSAANASEAWEKAVKKCIGRT
                     ALKREDEQILLSFGKSLGSTDLEGQIKNIRLALGQLALQVEKAEENRKKNESMYKSLG
                     ILGGLALVIILI"
     gene            20713..20910
                     /gene="spoIIIAC"
                     /locus_tag="PLG72_00790"
     CDS             20713..20910
                     /gene="spoIIIAC"
                     /locus_tag="PLG72_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOJ79371.1"
                     /translation="MGIDLIFRIAAIGILVAVLNQLLIRSGREEQAMMTTLAGLIVVL
                     LIIVKEIAVLFDTVKTIFRLD"
     gene            20954..21340
                     /gene="spoIIIAD"
                     /locus_tag="PLG72_00795"
     CDS             20954..21340
                     /gene="spoIIIAD"
                     /locus_tag="PLG72_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HOJ79372.1"
                     /translation="MEIVQIIGIGLVSTIIMIILRKQKPEIAVLAGIAAGALMFLLIA
                     AKLSAVVDLLEEYAARADIRPMYFSAVLKITGIAYIAEFGAEICRDAGEGAIAAKIEL
                     VGKVIIVTLAIPIITSLLDLVLKIMP"
     gene            21357..22580
                     /gene="spoIIIAE"
                     /locus_tag="PLG72_00800"
     CDS             21357..22580
                     /gene="spoIIIAE"
                     /locus_tag="PLG72_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOJ79373.1"
                     /translation="MVYIRVMKVLLPVFLSVLAAVLLMADDCRAERDTDAMDAMKTET
                     ADILGEQIEAGGIGRIGHELGKYTDEETMEMFEGFDAERLISDLAAGTADMDLKGTGR
                     KLLRFFFRELYQNIGILVKITVLVVICALLKNLQTGFMSEGAGQIAFYVCYIVLVSML
                     LVSFGTVMKMGIGIIDRMIGFMYASVPVLMALLISGGNITAGGILHPVMLLIVEISAT
                     LIRNVFVPLVFISAILTIINNISEKIQLTRLVNLIKQIVSWSLGIILTVFIIAISIQG
                     SLGAVIDGATQKAAKFAISTFIPIVGKTLSDAADTVIGCTLMIKNAAGIGVMAGILLI
                     CVLPLIKIIALAGLFKAAGALLEPISESRITNCINDIAGSMIHIFALSASVAFMFVVS
                     VTALISAGNISAMLR"
     gene            22608..23306
                     /gene="spoIIIAF"
                     /locus_tag="PLG72_00805"
     CDS             22608..23306
                     /gene="spoIIIAF"
                     /locus_tag="PLG72_00805"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HOJ79374.1"
                     /translation="MLEFIKNWTANIVVLVLFIVITEMLLPKGKVKKYANLMTGTILI
                     IAIIEPVTGLFGKSFDFTFSQAVTAGSIDKKEIEKAGRMFEEEQVRQTIRLYRNRIIE
                     QIEHQAREVEGVKDAKADVIINEDSSSPSFGEIKRIYIEAEVEGGPAGHNSTDNQDGS
                     GAFGGSVPANDGAPDVVMVPGIREVERIRIGGLDGMKRKASVDRRLEERLSERIANAF
                     GVNREHIIITQVAR"
     gene            23311..23928
                     /gene="spoIIIAG"
                     /locus_tag="PLG72_00810"
     CDS             23311..23928
                     /gene="spoIIIAG"
                     /locus_tag="PLG72_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HOJ79375.1"
                     /translation="METVYKIIGRIREWIARQDRKKLAENTAIVIIIGVIIIIAGSAI
                     LAPRKSRQDAPVQETARSGTAHADIVQDAEKEGDIETRLKSILSQIEGVGKVDVMITY
                     SSTAEKVPAYDIKRSQDETEEKDSAGGTRKIRGEEYESSLAYEDMSSGGKSPVILKKL
                     EPEIRGVLVVAEGAENIEVRDRICRAVTVVLDVSANRVQVVQRKK"
     gene            24055..24645
                     /locus_tag="PLG72_00815"
     CDS             24055..24645
                     /locus_tag="PLG72_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOJ79376.1"
                     /translation="MMILKRKQIVILALILLIVVAGYLQYSYRQGNTSADSEGKLGEA
                     LYVDNEIINEGMAGSVSDLADEGFGDQVNVTASKMANDYFIQAKLDKEQSRSEAADSL
                     KAIAEDASVSPEIREEAHSKMIALAETVEKESRIEILINKMGFEDSFILFADNGSVDI
                     IVKTPSLTSSQAAQITDIVMRQADVDVEDIHISTMF"
     gene            24737..25141
                     /locus_tag="PLG72_00820"
     CDS             24737..25141
                     /locus_tag="PLG72_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ79377.1"
                     /translation="MEENVQSVSNDIGTIKITDEVVAIIAGIAAMEVPGVTSMSGGIA
                     GGIAEALGRKNLSKGVKVEVGEKEAAIDLYIIVEYGYRIPEVAWTIQEKVKNAVEDMT
                     GLKVVEVNIHIQGVNIGSEHRKELLEDQSKQK"
     gene            25404..25964
                     /gene="amaP"
                     /locus_tag="PLG72_00825"
     CDS             25404..25964
                     /gene="amaP"
                     /locus_tag="PLG72_00825"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOJ79378.1"
                     /translation="MKIFFRILLAIYAFCLAVFSAFAMYVAIQPAAYVDISEQIIQAI
                     STDNATALRIAVFAVALVFFALSIMFLLSGVRSSKDKKAVSKHTSIGEIRISLNSIEN
                     IAINASKKTNGVRDSKTMVRKTDDGVEIEIRVVVMPDMSIPAITEDVQQRVKKSVEDT
                     AGVAVKTVKVIVDSIYSGITYKPRVE"
     gene            25977..26207
                     /locus_tag="PLG72_00830"
     CDS             25977..26207
                     /locus_tag="PLG72_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOJ79379.1"
                     /translation="MDTEKILEFYNEHRGAIIGAAAGLLLAVFILLIGFFRVLFIAIL
                     TGVGYYIGKKMHEDKDYIKNLLDRVLPPGTYR"
     gene            26248..26673
                     /gene="nusB"
                     /locus_tag="PLG72_00835"
     CDS             26248..26673
                     /gene="nusB"
                     /locus_tag="PLG72_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ79380.1"
                     /translation="MGRRAAREMAMKLLYQMEIQKDDIESQMKLFFEHNPVSENDTSY
                     IRDIIYGVYRNSEYISDIIQKHSRGWKITRISKIDLSILRLSIYEICFRDDIPFSVSV
                     NEAVELAKKYSSEEAGSFINGILSRLSDSRTGAGIENGC"
     gene            26678..27898
                     /gene="xseA"
                     /locus_tag="PLG72_00840"
     CDS             26678..27898
                     /gene="xseA"
                     /locus_tag="PLG72_00840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ79381.1"
                     /translation="MTSTNILTVSEVNKYIKDMISRDGILSGLWVKGEISNFKNHYSG
                     HFYFSLKDEKSVLKCVMFRSNASLLPFVPEDGMKVIIRGYVSVFERDGQYQLYAEEIQ
                     PDGIGALYIAFEKLKKKLEAEGLFDAAKKKKLPYLPKAIGVVTSSTGAVIRDIINVLS
                     RRFYNFNLKLYPVQVQGEQAAGQIAAAVRRFNELDNVDVIIVARGGGSLEDLWPFNEE
                     IVARSIYESRIPVISAVGHETDFTIADFVADVRAPTPSAAAELVMPERAVVENRLDSL
                     KLRLRNAVMKKTAMERLLLRKIEGSVAFRQPLNKIYQERMLLDVQKKYMQKALSALNT
                     NYRNKLSLLAATLDTLSPLKSLARGYSITKSKKDGSLVRSVHAVSIGDRLEINLMDGR
                     LGCTVESIEEDSNG"
     gene            27891..28130
                     /gene="xseB"
                     /locus_tag="PLG72_00845"
     CDS             27891..28130
                     /gene="xseB"
                     /locus_tag="PLG72_00845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ79382.1"
                     /translation="MDNNEVTFEQAMAELEEIVKKLEKGELTLDESINFFRRGVELTK
                     YCNRKLDEAERSITMLIEGENGVIEKELPELDQGR"
     gene            28155..>28631
                     /locus_tag="PLG72_00850"
     CDS             28155..>28631
                     /locus_tag="PLG72_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ79383.1"
                     /translation="MYGTTDFRKKYEEYANIVNNALENCIATDDKPGSEIYNAMKYSL
                     AAGGKRLRPVLALAVCDILGGNRNDILPFACAIEMIHTYSLIHDDLPAMDDDDYRRGI
                     PSNHKVFGEAMAILAGDALLNKAYEIMISHIMKKEGDLKAYVRAMDVIAVSAGAEGM"
BASE COUNT         8177 a         6039 c         7921 g         6494 t
ORIGIN      
        1 aagcggcagg agagccacag gcagtgcaaa taagaagcgc ggcggaagag tgataatgtt
       61 ggagggcaaa aacccgggaa cacccgccgc tgttaaagat attgctccgg atgacatcgt
      121 gtttgccatg gatataggca cccgaaccat cgccggtgtc atcgggagac aggaaggaga
      181 cgttttccac gtacttgcca cagaagtttg cgaacacaaa agcagggcga tgatggacgg
      241 acagatccat gacatagacc aggccgctgc tacagccatg gaagtaaagc gcaggcttga
      301 gtataggata ggcttcaggc tgaaaagagt ggccatcgcg gctgccggaa gagtgctcag
      361 gacatgccag gtcaaagtgg agaaaaacct ggaacaggac atggagatcg acgccgatct
      421 ggtgagcagc cttgagatag aagggatcca gcgcgcccag acggtccttg acgaaaactc
      481 cgaagctgaa gataaaaacc agtattactg cgtcggatac agtgtcataa actattacct
      541 gaacggatat gtgatatcga agctcacagg acataagggc aggactgccg gcgtagaact
      601 gcttgcaacg ttcctgccgc ttacggtcgt tgacagctta tattcggtcg tgaacaggct
      661 gggcctggag gtcgcaagcc tgacccttga gccgatcgca gccatcaatg tggcgatagc
      721 tccggacctg aggctgctga atcttgcact tgtcgatata ggcgcaggga cttcggacat
      781 cgctcttaca aaggacggaa gcgtgtttgc atacgcaatg gcgtctgtcg caggagatga
      841 aattacagaa agcatatcgc agcgttatct cgtggatttt gccacagcgg aaaagatcaa
      901 agtatcgctg tcttcaggaa aggaaaaaat ccggttcacc gatatactga aaaagaagca
      961 tgagataacg gcagaggaag tcctgcgcga tatcggggat acgatccgga tgcttgcatc
     1021 ggcgatttcc ggcaaaatac tggagtacaa ccgcaaagct cccaatgcgg tgttccttgt
     1081 aggaggaggg agccgcatcc cgctgctgac gaagttcctg gcggagcagc ttggtttgcc
     1141 ggaagacagg gtcgtagtaa ggggacgcga tgtggtcagg ggcgttaagt acagcgacaa
     1201 aaaactgtac ggccctgaag cggtcactcc gttagggata gcggtcacag cgcagatgta
     1261 tgcggggaag gacttcctgt ctgtaacggt gaacgataaa aagctgaagc tgttcaactc
     1321 taaaaaactg aaggtttccg atgcgcttgt actttatgga ttcaatggag gggatctgat
     1381 aggacgttcc ggaaagagca taacctgcac tgtcaacggc gaagagcgca ggatccgcgg
     1441 cgaatacggg aaagcggcgg agatctggct gaacggtaaa ttgtcaagcc ttgatgcttc
     1501 attgtcattc ggggacaata tcaccgtaat accggctcag aacggcaggg atgcatcggt
     1561 gaaggcatat gaacttgtgc ctgattactg caccggctct gtcatcctga acggcagccc
     1621 ggtggatata agtacgataa taaccgtcaa tgggagacag gtcggcgctg aaactgaaat
     1681 aaaggatgga gatacagttc agttttcgag aataagaacc cttggcgatc tgctggaagc
     1741 agcgggtttt acaggtatgg actgcaatat cacggtcaat gggatcagtg actgcaggta
     1801 cgattatgtg ctgaaagaca gggatgtcgt gatatgtgag gaacgacgcg aggattactc
     1861 gccggctgcc tcagaagatc atggcggaag tgtcggaaac ctgcacaaaa acggggctga
     1921 gaccgaagcc gggatcggaa ccgggactga agctgaaacc ggggacatga tgaacattta
     1981 tacgcggcag gaaccggacg aatggttcga tgttactgtg aacggaaaaa ccgtttcatt
     2041 gaggaaaaac caggcaaggc atatgttcgt tgatatattc aactatatag atttcgacct
     2101 gtcaaaaccg cagggaacga tagtgctgag actgaacggc aggcctgcgg cctttaccga
     2161 tgtgatcggc gaaggagacg aaatagagat atattggcga aaatagcatt ggggggtata
     2221 ttaactggac catttcaaag gcagaagcct gaacaaattc gaagcatttg agcttattta
     2281 cttaaagaca tcggtggtat ttctcatcgc atcggctttc atatatttta ttgaaaacat
     2341 tttactgaag gatgtatcaa taacatctga tcctgcctac cggatacgga tgatctggag
     2401 cgcagccgca tgcgtgaccg tttttgcact gctgctgata agatacaagg ttttccggag
     2461 tttcatgctt aataacatat tggtatactg ccttgaaaaa gtactgttca gttcgatagt
     2521 atctgcgata gccatatttt caggtatagg cgtgtggttt tatataggct atctgctgcc
     2581 aatgtgcgct acgacataca taaaagggaa gaaatacgga tatgccacgg cggtcacgtc
     2641 cgccataata cacatgctga tgctgtacgg caggatttca ctggcggagg caggcgggat
     2701 cgcctcggat gtcaaaaaga caataggcat ggctgcaggc ttatatgtaa tatctttcat
     2761 aaccgtatac ttcttcggcg aattatataa aaacaggatc gaggaagagt ttcaggccac
     2821 atatgccacg gaacagttca atgaacgtta ccagatgctt aaaagcgaaa tggatgagat
     2881 catacagaaa tacagcagcc tgataaccaa ttcggataag ctggaggaat ccaacaaatc
     2941 cctttcaaaa agcatcgcag aattttatac tcttaaccag atcagccagg ccatcggttc
     3001 gatccttgat accagggaac tcctgaaacg ccttaatgac ataatccttg gcgtcgtcgg
     3061 aggcagttat tccacaatag tgctgtacga tgaagaatcc gggcgcctga aggtccatac
     3121 gacgaatgtc acgaatcctg ccgaacttgc tacgctgatg gacaatatca actgcagcat
     3181 cctgatggat gtactcatga aaggcgaatg cctgctcgag aacaatgttg actattatca
     3241 atacctgttt acctgcggca ggaacataaa ctcgctgatg tgcataccgc tgacgacaag
     3301 ttcgagaaaa tacgggctga tacttgtcga acacacgatg agcaatgtgt ttgatactga
     3361 aaacgtgaga ttcctcagga taatcgcgca gcaggtcgga atagtcatgg agaatgcgga
     3421 gctttacctg aaaatgaagg agatggcgcg gaaagacgga cttaccggga tttacaaccg
     3481 ccagtatttc caggagagac tccaggccga gttcagggaa gcgcagaaaa atggctatcc
     3541 gctgtcgctt gtcatgtatg atatcgacca cttcaagaaa ttcaatgata tgtacggcca
     3601 ccttttcgga gacaaggtgc tgaagtctgt tgcagaggtg gtcgcatcga cgctgagaaa
     3661 aggcgacatg ctggccaggt tcgggggaga agaatttacc atactgctcc cgcagacaag
     3721 cctggatgaa gcatatgaaa aggttgaaac attgaggaag ctgatatcgc agcatgtcat
     3781 agaagataac ctgattaacg tatcagtgac cgcgagtttc gggatatctt catatgatga
     3841 atgcgctctt aatgaaaacg accttgtacg tacggctgac tatgcgcttt atgaggccaa
     3901 ggcgtcaggg cgcaactgtg taatgacggc caggagactg tacgaagtca ggcactgata
     3961 gtggatactg acgaccgggt ttttgtattg ataggtctgc ggacgtgcac aaaacatttg
     4021 ccacggagta ttgtgatatt aacagaccta tcggggagga aacgtttatg aggataatgg
     4081 tcgtcaaact gcccagattt ttcggcaatc tgatcaggaa agtgctcagg atgaactgaa
     4141 atgcattaaa aatcaaagcg acataaaaga ctttcgctaa agcttttatg tcgcttcgat
     4201 atatatacgg aaacattgct gaccgtacga tggctgctca gacggccctt gtgaccttgt
     4261 tggagcgcag gcatctggag caaacagtga tggtcttggg tgtgccgttc tctatcaccc
     4321 tgactttttt tatgttgggc ttccatgccc tgtttgtcct tcttacggag tgactcacat
     4381 tattgccgaa tacggtacct ttatcgcata tcacacatct tgccatctat atccacctcc
     4441 ttaaagccgg agcttatgat cgcaaaagca taactatttt aacacaaccg ttccgaatac
     4501 tgcaagtgct ttttatctca atatgcaggt attgtatcag tttgtaacaa aacagtgagc
     4561 gtggcagcat aaggacaaag tagtcatgca ttaccttacg gaagcgggag cggtgtccag
     4621 ccactattcc tacatccaca tccataatgc atggctggat acctcctctc aatatttcag
     4681 ccggtcatcc ttggaactta tcaggacttg actgcgtcag gttaacctga attacggtgt
     4741 ggaaaatcca agcatgatat attagaatta aatatatgac tattctgcgg actggagatg
     4801 tgcaatgcac aaaggctcgg aactgctgaa caaacaactt caatacctga aaggcgtcgg
     4861 ggaggcaagg gctgctctgt tcaggaggct tggggtccat acggtgggag acgtgatctc
     4921 ccattatccg agagattatg aagaccgcag caggctgaaa aagataattc agttgcagga
     4981 cggcgatcaa tgttcttttg aagggatcat cgcatcaagg gtagtcgtgt cgaagcccag
     5041 gaaaggcctt gccataagca aagtgagcat cagggacgat acaggcctga taaatgccgt
     5101 atggttcaac aagccgtacc tgaaggacca gctgaagccc ggagaaagat accttttctt
     5161 tggaaaggtg acaaggagag gaactttcga ggtactgaac ccggtatacg aaaaaacgga
     5221 tgagtcaggg cctgtgaata cgtgcaggat cataccgata tatccgtcga caggcaaact
     5281 gacccagaac gtcatccgct ccgtgatcag gaatgcattg gcttatgccg gcgacagcat
     5341 tgaagacatt ttgcccgggt ggatcaggga acggtatcat ctggcaggcg ctcagtattc
     5401 gatcagcaac atccactttc ccgaaagtga cgaagccttt ctgaaagccc gcgaacgtct
     5461 cgtctttgaa gaactgctga tactccagct tgccctgatg tcagtgagga ccgttcagga
     5521 aagcgccgtg cccgggatca cattcggcgc ccgggaagaa gtccgcagat ttgtcgaggg
     5581 attgccgttc aggctgacga atgcgcagct aagggtactt gatgagatcg aacgcgacat
     5641 ggaaagcagc aaggtcatga accggctcgt gcagggcgat gtcgggtcag ggaagaccag
     5701 cgtcgccgtc gctgcgcttg taaaagctgt gaaaagcggg taccagggag ctttcatggt
     5761 gcccaccgaa atacttgccg cccagcatta ttcatcgctg aaagatctgc tgggaccgct
     5821 gggcttcaat atagccctgc tgacaggcag cgtcgggacg aaggatgcca aaagggtaca
     5881 ggcgggcgta tcttctggag agatagatat cgccgtaggt acccatgctt tgatagaaga
     5941 taaggtggtt ttcaataacc ttggtctcgt tatcaccgat gagcagcaca ggttcggtgt
     6001 aaggcagaga gccgcgctgg ggaagaaagg ggcctgccct gacatgcttg tcatgacggc
     6061 cactccgata ccgaggacgc tggcactgat attatatgga gacctggata tttcgatcat
     6121 cgacgaactg cccccgggaa ggaaaaaagt gcttacatat gcggtggact cgtcgatgcg
     6181 tgaaagggtc tataatttta tcaggaaaca ggtagctgag ggaaggcagg tctacatcgt
     6241 ctgtccgctg gtggaagaat ccgatgccgt tgatgccgtc tcggcttccg aactggcttt
     6301 ccagttggcc cacaaaacat tcagcgacca taaggtcggg ctgatacacg gcaagatgag
     6361 gccgtccgag aaggacgacg taatgaggag tttcatatcg ggggaaacga gtatcctggt
     6421 ttcgactacc gtgatcgagg tcggcgtcaa tgtgccgaac gcgacgctta tggtgatcga
     6481 aaacgcggaa agattcggcc tttcccagct gcatcagctg agaggcaggg tcggaaggag
     6541 cagttaccag tcatactgta tactgttcaa tgacagtaaa tcagaaatag caaaagaaag
     6601 aatgaaggta atgacacaaa cttccgacgg ctttgttatt tcggaaaaag atctggaact
     6661 gaggggcccg ggagatttct tcgggaccag gcagcacgga ataccggatc tcaagatagc
     6721 taatttgtac aaagatatgg aaatacttaa aaaagcacag gaagctgcgg agatgctcat
     6781 cagggatgac aggaagctgg aaacgcgtga aaacctgctg ctgggccagg ctgtccgtga
     6841 aaaatacagc ggagtaataa attccataag tatgaactga agtataaata atgcaggcat
     6901 aggggaggaa tctgttattc tcagggtaat atcaggttca gcaaaggggc ataatctcaa
     6961 aactctgaaa ggcatggcca cgaggcctac ttccggcatg gtgaaggagg cgctgttcag
     7021 tatcatcgcg ccaaaggttg gagacagcaa agtactggac ctgttcgcgg gttcaggggg
     7081 ccttggtatc gaggctttga gcaggggagc ggcctgggcg gtatttatcg accggaaccc
     7141 gaggtgctgc agcataataa aggaaaacct ggcacatacg aaacttttgg atttcgctga
     7201 agtgtatgca attgattatt cggacgccat ccggaaaatg cgcagggaac agcgtaaatt
     7261 tgacattata atcatggatc ctccatataa taaaaatttt gctcaggaag cgttaaaact
     7321 attgacaaat aatgatataa tgaaggacga tggaataata atcgccgaac acagtgcttc
     7381 agacgatctg cctgagacct gcggcaggtt cagggcgatc gtcaggagag ggtacggtga
     7441 tacgatgatt acgatataca aggatggtaa agacggttta tgacactggt atatcctgga
     7501 agtttcgacc ctataacaaa cggacatctc gatatcataa taagagcagc atgcatctgc
     7561 gacgaactta tcgtggcagt agggaacaat gaaagcaaaa agcccgcatt caccgtggaa
     7621 gaacgggtag agatgataaa ggaatcgata ggcggcaggg aaaatatact tgtaacgagt
     7681 tttacaggat tgcttgctga ttttgcgaaa aaggtaggag cccgggccgt cattaagggc
     7741 ctcagagcta tgtctgattt tgaatatgag ttccagatgg cgcttctgaa caaaaaactt
     7801 gcgccggaac tggagacgct gtttatgatg accagcgtac aattttctta tctcagttca
     7861 agcgccgtaa aagagatcgc ccggaacggc gggtgcattg acgggctggt acctgactgc
     7921 ataaaggaaa ggatagttgg taagctgacc tgcagataga tcattgggag gttaaaacca
     7981 tggaaattct ttcgatactt gagacattag aagatttggt cgaaaagagt gttactgttc
     8041 cttttacagg taaatgcctc gtcgacaagg aggagatact tgagatcgta aaggaactca
     8101 ggctgaagct tcctgacgat ataaagcagg caaagtgggt gaaggaagag cgccagagga
     8161 ttcttctgga tgcccagaaa gaagccaaca acatgatcaa ggacgcagaa ggaaagatcg
     8221 cgtccatggt gaacgagcac gagataacca aaaaggctta cgagcaggcc aacgagatcg
     8281 ttgccgcagc gcagaagaat gcccgcgaga tcaggctggg cacaaaagag tatgctgaca
     8341 atatacttaa taaagtggaa gaaatactga ccgatacact ggccgttatc aaggcgaaca
     8401 gggaagagct caaatagggc tctttttttt catggcttta aaaagcagga tcaaagagat
     8461 gataccgatc cacacggcaa taacaacggc aagtatcaat gacgaatgcc cgatcgtccg
     8521 gacaaaaaag tgaaaatcga atgccggcag gccaagagtc ggttgtgctg ccacgcttcc
     8581 gggcggcagc agacccatgc ccgcccaggc atatatcgcg gctgcagtac catgcagcat
     8641 tttgcccaac agatatggcc ggatatttat atctgtccct gaaatgatgc ccatgacctg
     8701 gagatggacc gagaggccgg cccagccaat tataaaactg acggcaggca gctttacatg
     8761 gagaggaacg ttgacgcatc ggctgacagt tccggttccg gtggttattt ccagcaatcc
     8821 gttcaaaact cctgatacga caccattcat atcccatttc agcatatatt cgggcatcag
     8881 ccgcaggact gccgatactg ccagcctgat aaggcccgtt tcctcaagga agctgataac
     8941 gaccgagaaa agtacgatga acccgccgat ggaaaggatc gtggcgacgg aattcctgac
     9001 cgcgtcgccg agcagtatgc ccgggttttt ccatgtattt tcgcccattt tcagtttttt
     9061 gcccggatca gtcctggcaa ttttcctgtt ttgactgccg gtaccaaatg gtcccatgga
     9121 atgccttttg ccttccaaat cagcggcgga ccttggaaca ggtatgtttc cgggtacgga
     9181 aggcgttttt cctgattgcc tgtaaaacct gaaaaccaac ccgacagtga gggacgccag
     9241 aaggtggcag atgaacagga aatagccgat ggccggcgat ccgagcatgc ctgtgcctac
     9301 ggcgcctatt atgaaaatgg ggccggaatt gtttgtaaag gcaagcagcc gttctgcttc
     9361 agttttggtg atagccttgt ttttccttag atccgctgtt atcttggcgc cgaccgggta
     9421 cccggatgtc acgccaagag ccagggccag ggaagagcag cccggcaggt tgaacaatgg
     9481 tctcatgaca ggttccagca gtgtcccggc catgtatgtg aaaccggatg agctcaacag
     9541 ttccgaggct gcgaaaaaag ggaaaagtga cggtacgact acggcagtcc agaggttcag
     9601 gccttttagt gcagcttttg cagccgtatc ggaaaaaagg accaggcaca ctgtgaaaat
     9661 gacgcataat gcgggaaagg ccagccgccg cagatatatg gtcatgggat gccgcacgga
     9721 tattagtagc gctaccgaaa ccaggatcag gacaagaaga gtgtatatca ttgccaaact
     9781 gcctctaaaa tgctactaat gaattattat tatcggttcg gcgaaatatt cctgataaga
     9841 gatatgctgc tgtccactgc gagtggatcc atggacatga caaaaggcag tcatctgccg
     9901 taactgcagg gcgcagcgac gccagatgac tgcctttact ccggacggtc ctattcgacc
     9961 acggttatcc atccgaactc gtcttttacg gtaccgttct gtatgcctgt tatcgtattg
    10021 tatagtttca gtgaaacttc tcctatcttt ccgtcgttta tggtaatgac ctttccgtcc
    10081 cagttgagtt cacctatcgg tgagatgacg gcggctgtgc ctgtaccgaa agcttcatcc
    10141 agttttcctt ctgcatacgc gtcaaacagt tcctggacgg caagctttct ttcatttacc
    10201 ttgtatcccc atttcctgag caaatggatg ctcgaatccc tggtgatgcc cggcaggatg
    10261 ctgccatcga gagcaggtgt tacgacctcg tcgccgatct tgaagaaaac attcattgaa
    10321 ccgacttctt ctatgtattt cctttcgacg ccatccagcc agagaacctg cacatagcct
    10381 ttctcatagg ctatttcctg tgacttgagg cttgccgcat aattgccggg tgtctttgcg
    10441 aaacctgtac cgcctcttac ggcacgcaca tattcattct cgaccattat tttcacgggg
    10501 ttgatgccgg tcggatagta tgcgccgctg ggagagagta ttattatgaa tttgtacgta
    10561 taggacggcc tgacaccgag ataaggatcg gtggaaatta tgaaaggcct gatataaagc
    10621 gatgttccgg gttctgacgg gatccagtcc ttatctatgc tgacgagggc cttgaccgct
    10681 tccactccga attcctcatc tataggcggt atcgacatcc tgtcgttcga cttgtttatc
    10741 cttttcatgt tcatgtcagg tctgaagagg aaaatcctgc cgtcagccga tttgtaagcc
    10801 ttcaggcctt caaatatcgc ctggccgtaa tggaatacca tcgtggaggg ttccagacac
    10861 aacggaccgt acgggactat gcgcgggtca tgccagccct tctccggatt gtaatccatg
    10921 atgaacatgt gatctgtaaa ataatgcccg aaaccaagat ttttttgatc cggcttcggc
    10981 ttggggttct gcgttttctg gatttttatt tcctgtgcca ctttacacca acctcctgac
    11041 atttatttgc tcaaaatatt tttttctatt atgcatcttt ttttctgaaa aatccatacc
    11101 tgcggcaaaa cttgttaaat ttaagcaaaa tatttttaac atattcacaa aatggtatat
    11161 aatgaaatat ggtaccgcaa atcgtgctga ggagggtcag atatggacaa caacgtcgct
    11221 tccaggagga atgtggatta caggtccgct tcaaggaggg tcaacagatt tgtattgctg
    11281 ctcaactggg cgatcgacat tttcctgata gcaggatatg cgctggaata tctgaagggc
    11341 ggcaaaaagc tttcatatct gatggtgatg tcggcgatag tcataattcc gatggtcata
    11401 gccacaatca tattccggaa aaacaaccga tccaacataa tgaagtatgt gacgctgtcc
    11461 gggtatttca tactttatac atttgtgatg ttcactgcgt cgcctggcag gctgcaggtg
    11521 tttgcatata tgtttcctat tgtgctcagt tatttcctgt atttcgacct taagctggtg
    11581 cttataggcg gcaccgcttt cacgcttgtc aatattgcga ggataatctg gctgatgttt
    11641 ttcctcggac aaaacagcag tttcgagact acggattacc tgatacagtt tggctgcgtc
    11701 ctgacatttt ccttatcgct catatattcc acgaaaatat ccaatacgtt cagtgatgag
    11761 aaaataatgg atataaggac agaacaggag aaccaggaaa ggatactcag ggatgtgctg
    11821 aaaatagcgg cggtactgga caggaactcc aaggaggtgc accgtatagt caaggaactg
    11881 gaagaaatca cgaatgtagc atcgaacgcc gtgcaggaaa tagaaaaagg cgcggccgat
    11941 actgctgcaa atatacaggt gcagtccggg ctcacccatg atatacacca gcttatcagc
    12001 gacacttcga aggattcgga aacaatggag ctgatatctg tgaatactgc tgaggcagtg
    12061 tccgagggta tgaagataat cgagatcctt aacgggaaat cggacgatgt cagccagaac
    12121 agcgacagtg cttacaatat aatgcttgac ctgaaaaaga agaccggcga gatacgaacc
    12181 ataactgacc ttatctcgag catatccgag cagacgaacc tgctgtcgct gaatgcagct
    12241 atagaaagcg caagggcagg cgaggcggga aaggggtttg cagttgtagc tgacgagata
    12301 aggaagctgg cggttcagag caaggagtcc accgacagca taatcaagat tataaacgac
    12361 ttgtacgaac agaccgacag atccgtggaa gccgtgctga aactcaagga agcaaatgaa
    12421 gagcagaacg cgctggtttc gaggaccatg gagattttta cgagcatcag ccggaaaatg
    12481 gacgaagtaa gggaaagcgt caacagggta aatgaaaaag tcagcaagat ccttgaagca
    12541 aacaacagga tggtggaaag cataaatgag atatctgcgg taagcgaaca ggtaactgcc
    12601 agtgcagagg aggcatgcgc cctctccgcc caaaatatag aaaaggcgaa agaggccggg
    12661 atgtatgtgg aagagctgat cgaaacttcg atgcagatga gcaaatacca gaactgatat
    12721 acttctgacg gaacagggac tgatggagga gcgatgaacg agatatttct gcccaggatg
    12781 tatgttgaca gcttcgctga aattaccgcc gacatactga gaaagaatga tatacaaggt
    12841 ctgatacttg acgtcgataa tacgcttgtc ccgaatcacg tggccgatcc cgacgacagc
    12901 gtgctgaact ggatagaatc gatgaaagca tcgggtttca agctttgcat agtttcgaac
    12961 ggtgcaaaaa ggcgtgtttc aaggttcaat gaaaaactga agctgtatgc gatacacaga
    13021 gctatgaaac ctgtaaaaag tgcttttctg aaagccggca ggcttatggg ccttgagagc
    13081 cggcacgtcg ctgtcgttgg agaccagatc tttacggatg tttacggagg caaccgtgcc
    13141 ggcatgttta ccatactcgt caggcctata gaccagcgcg agggcagact ggtgaagctc
    13201 aaaaggatct ttgaaaagcg tatactcagg ggatatgccc cgaagacaaa gtaacgggcg
    13261 gcaatgagga gtttgcagat ggtgcttgat aagagggtcg acggcagaac aaagctgaca
    13321 ggactgatag ggaacccggt ggaacatact gtttcaccgg tcatccataa cagccttttt
    13381 tcgtatttgg gcataaacgg gatatatatt ccgatgaaaa ccgatgaagc aggccttgag
    13441 acagccgtca ggggactggc ggcacttggg ttcacagggt tcaacgtaac gataccatac
    13501 aaggaggctg tctgcgatct cctggatgag accgatgatg aggtaaagtt gctcggagcc
    13561 gcaaacaccg ttagaatcaa agacggcagg ctgtatggat ataacaccga cggcgaaggc
    13621 ttcgtgacag cattcaaaaa acagaccggt tcggattttt cggggaaaaa gatctgcata
    13681 ctgggagcag gaggcactgc aaggacgctg gctgtaaaag ttgtacagga gggagccggc
    13741 gaagtctgca ttataaacag gaccgtaccc aaggctgacg agatagccgg atatatcaac
    13801 aggaccctgc ttaagaacag cggcttaaga aagcccgtct ttactgcagc gcccggaacc
    13861 ggccaggcac acggggtgct caacgggtcc gaaatcatta tcaatactac atccgtcggc
    13921 atgttcccga atacggacgc aagccccgtg ggagataatg tggattacct gacaaaaccg
    13981 atagtttacg acgtaatata taatcctgcc gagaccaggt tccttgcctc ggcgcggatg
    14041 aaaggctgca ggacatacaa cggggccggc atgctgttct accaggggat caaagctttc
    14101 gagatctgga tggagacaaa ggtacctgat gatgttttac aggaactttc gacatcattt
    14161 ttgaaatatc tggaggttta aagggttttt tcgtctgtac ggcgaataaa ataatgaatt
    14221 tatcggacag gtgaagaaat ggcggcttac tatgtattca ttttcattct cggactggtt
    14281 gtggggtcgt tcgtgaatgt cctcatatac agagtgcctc tgcacaaatc catcgtggct
    14341 ccgccgtcgt catgcacatc ctgcggggaa aggctgaccg tcccggacct tataccggtc
    14401 ttcagctaca ttttcctcaa gggaagatgc aggcactgcg gggagcgcat ttcgccaagg
    14461 tatccgctgg tggaactgct gacggctgcc atgttcgtca tcctgttcac caggttcggg
    14521 tttacggtgc ccttcattgc ttatgcttac cttatgacga tactggccgc agtgtttttt
    14581 atagatatcg accacaggat aataccaaac ggcctggtgc ttgcagcttt ggctggcggt
    14641 ttggcgtttt ttgcatggaa ctgcatatct ccggatcctt tggttttcgg agatgaaaaa
    14701 tggtggacac cgttggccgg cctgcttcct ggatcggggt tcctgctgct ggtcgccata
    14761 ttgggctcac tgatatacag gacagaagat gccatgggga tgggagatgt caagctgatg
    14821 gcgccggtcg ggctgttcct gggctggagg ctttgcataa ctgcacttgc ggtatctgta
    14881 cttttgggcg gcctggtctc gatggcactg atacttcttg ggatcaaaaa gagaaaggat
    14941 actgtggcat tcggaccatt catagttgcc ggtacgttcg tgaccatgat ctggggctgg
    15001 gagctcacag aatggttctt caactatata aatgcataag gcttaagcca aagcaaattt
    15061 aacggtcagg gaggaaatta aaaatggtac agatcaaaaa ttatatggaa gatgtcgtat
    15121 tcggacaaat gaaggaagta ctcgcggaca tcaatatgtg cacatgtgag aaatgtcttc
    15181 tggacgttgc cgccatagcg ctcaatgacc ttccgcccaa atatgtggtt actgaaaagg
    15241 gagaactgta ttccaaggta aacacgctcg gacagcagtt cgaggtcggt gtggtggctg
    15301 cgctcacaaa agcggcagta ttggtgaaga gaaggccgcg ccacatctga tccggatgcc
    15361 cggaccagtt ggataactcg gtgatgaagg ggaaacataa cgatatgagg aaaaggatca
    15421 ttgtaatcaa cggtcccaat ctgaatctgc tcggaacgcg ggaaacggac gtgtatggga
    15481 cagtcacgct ggccgaaata gctgcggctg tcgtcgaaga agctgacagt cttggcctgg
    15541 atgtggattt tattcagacc aaccatgaag gcgagatcat tgacaagctc catgagtccc
    15601 gcgggaaata tgatcttgtg atcctgaatc ccggtgcata cacccattac agcattgcga
    15661 tacgcgatgc cgtaaaagct gtggaacttc cgtgtatcga agtacacctt tcgaacgtcc
    15721 atgccaggga agagttcagg agcaagtcgg tgatcgctcc cgtatgcgtc gggcagataa
    15781 gcgggtttgg agccaacagc tatatcatcg cactcaaggc agccgcattg ctgctaagct
    15841 gaaagtaaag gagacgtatt actgtgacaa tatatggcaa cagactggac agtatcagga
    15901 gaaaaatggc atcatcgaac atcgatgcgg tattggtaac aaaacgtgtg aactatatgt
    15961 atttatcagg ttttaccggt actgcggcga tccttttcat aagcggagca cgcgctgtcc
    16021 tgctgaccga tttcaggtat gtggagcagg cagcgaagca ggctcctgat tatgaaatcg
    16081 tgcggtacgt gacggatcag tacgaggaaa ttaacaagct tcttgaaagc gaaggaataa
    16141 aaaggcttgc ctttgaagaa tgccatttgt cctacaagca atacacccag tacctcggga
    16201 aactgaaggt ggatgaattc gtccctctcg gaggcatgat cgaggaactg cgcagtatca
    16261 aggatgaaac ggagatcagc cttatcagga aagctgtgga tatagcagat gatgcttttt
    16321 cgtatatcct gggcgtcata aagccgggta tcacggaact cgatattgcg gacgaactcg
    16381 aacaccatat gaagcgcctg ggcgctgaag gcccgtcttt cgagaccatc gtggcgtcgg
    16441 gaaaacgggc gtcgatgcca catggggtgg cttccgaaaa gaaaattgag cacggggacg
    16501 tcgtgactct tgactttgga gcaatatata aaggatattg ctccgatatc acgaggaccg
    16561 tctttgtcgg aaaacccgag ggggagatcg agaagatata caggatagtg ctcgatgcga
    16621 acaggagggg cattgaggct gtcagaagcg gcatactggc aaaagaggcg gattcggccg
    16681 ccagggagtt cataaaggaa gcaggctacg gagataactt cggacacggt cttggacacg
    16741 gcgtcggcct tgagatacac gaagacccga ccctctcgcc cagaggggat atcgtgctta
    16801 aggacggaat ggtcgttacg gtggaaccgg gcatatatgt tacagacctc ggcggcgtga
    16861 ggatagagga catggtggtc gtaaacggcg atagtgcgct tgttctgacg aaatctccca
    16921 aggaaatgat catattgtga aacagcgctt ataattagaa atttttcctt gaaatacatg
    16981 gtattttatt ttaatattat atgggcatta tatgggcaat accgggaacc ggctatttgc
    17041 ccggatgagc caaatcgaag tcaagcaaat atcggaggta ggaatttatg ataacagcag
    17101 gcgatttcag gaatggagta actttcgaac tggatggaca ggtattccag gtagttgagt
    17161 ttatgcatgt gaagcccgga aaaggcgcgg cttttgtcag gacgaaactc aaaaacgtca
    17221 ttactggggc aaccatagaa aggacattca gccctacgga caagatgcca gaggcccata
    17281 tcgaaagaaa ggacatgcag tatctgtaca atgacggaga tctgtactat ttcatggata
    17341 tggaaaccta cgagcaggta ccgctgggag ctgatactgt aggtgattcc ctgaaattcg
    17401 tcaaggagaa catgaatgtt aagatcctgt cattcaaagg gaatgtattc ggcatagagc
    17461 cccccacatt cgtggaactc gaggtcgtcg aaacggaacc gggcttcagg ggggatactg
    17521 ccaccaatac gacgaagcct gcaacggttg agaccggggc tatcgtgaaa gtaccgctgt
    17581 ttgtcgacaa gggcgatgtg atcaggatcg acacaaggac cggagagtat cttgaaagag
    17641 cataagcgtt tgaataaatt tcctgcacgg ggagataata ttcagtacat tatctaatat
    17701 cacttacgga tccggtcatt gtaccgatca gtgatcatgg aggtgcttat gaactatctg
    17761 agggaaagag tagcatatct gaaaggactg gcagacggaa tgaagctgga tacttccacc
    17821 aatgaaggca agcttctcaa agctatactg gaagtgcttg atgatatggc cctggctgtc
    17881 gatgacatcg aagaggccca ggaacagctc agcgagcagg tggatgaaat agacgaagat
    17941 ctggctgaga tagaggacct tttatatggt gtggatgacg aggatgaaga agagtactgc
    18001 tatgatgagg atgacgatga agaagatgaa gaaatagtcg aacttgagtg tcctcattgc
    18061 ggagagacgg tcaatcttga agatgcattc atgagaggaa atactgtcct ttgtcctcat
    18121 tgcagcaaag agatcgagat agaattcgac tgcgactgcg aagactgtga agacaacgaa
    18181 gaagatgctg aggactgaag tacctggatt ttcagaagtg ctcagatatc gtgatgattc
    18241 aggcgggatc cggagacagt cgacccgcct tttgattttt ggggtgaacg gtatgagcgt
    18301 caaagcgttg ttgagggaaa taatcgaatg gatcgttata ttcggggcgg ccttcgtact
    18361 tgtggctttg ctgaatacag cggttttttc agccacgcag gttaagcaga cgtcgatgca
    18421 ggatacactg caggaaggtc agcacctgct gatcgaaaag atcagctata ctttcggcga
    18481 cccttcaagg ggcgatatca tagtgtttct tgagaacaag tacccggaga actatatcga
    18541 aaaggtcagg atatttatca ccgatgtagg agacatactg aaacctcccg aaagaaagac
    18601 caatatcagg atggtaaaaa gagttatagg agtcccgggc gacgaggtgg atattcgtga
    18661 cgggaaagta tatgtgaacg gcatcgaact cgaagaagaa tacgttaagg gagtgacgtt
    18721 ccagagagag cagccgatgc ctgtaaaact ggatgaggac gaatatttcg taatgggtga
    18781 caacagggag gtcagcaagg acagcaggac tttcggcccg gtagaccgca gccagataga
    18841 agggaaagca gtatttcggt tctggccctt gtccgggttt ggaaccctga agtaaccaca
    18901 cacgaaaatg acggatcaat cgaataatga taagaaaaca ggcctttgag cagggcctgt
    18961 tttttgtgtg cactttaagt cataaaagtg gacaaagcca aataattata agtaaacagg
    19021 caaggacgtc caacgttccg gagtctgaat acgatcgatg cggggaaacg gctatgaaag
    19081 aaaactccga cggcaaatta tcagtaaaag cgatgagcag cctcaggaaa gatatacttc
    19141 cgatgatttc cggaagagtt gccgcggcca tctcaggggc attgatctat ggactggagc
    19201 cgcttgagga aataaggctg agggcggaga aaccgcttgt actgcagaac tggtcccggg
    19261 aatggttcat cggcagtgac ggactgccgt ctccgaaacc ggcaggcagt tttatcgtga
    19321 cccaggaaga tatcaggacc acactggagc ttatgagcga aaattcggtc tatgcctacc
    19381 aggacgagat caagaacggg ttcataacga tcaggggagg gcaccgggtc ggtattgcgg
    19441 gaagggctgt actcgacggt gagtcgatca ggaacattaa ggacatatca gggcttaaca
    19501 tccgcatttc caaccaggta ttcggctgtt cgtcaaaggt tttgaaatat ctgatcaaaa
    19561 acggcaccga tatatacaat acgctcatca tatcacctcc acagtgcgga aaaactacta
    19621 tccttcggga tattgccagg aatataagca atggggtgcc ggaactcggc ttcaaagggg
    19681 tcaaggtggc aatagtcgat gagagatccg agatagcagc aatgctaagg ggcgtgccgc
    19741 aaaatgacgt cggccttagg acggacgtgc tcgacggctg ccccaagagc atcggtatga
    19801 aaatgatgat aaggtccatg tccccgaaag tgataatcac tgatgaaata ggcaatgatg
    19861 gggacaggga ggcagttcac agcgtactga acgcaggcgt caggataata accaccgctc
    19921 acggctacaa catttctgaa ctcaaaagca ggagggaagt cctaatgctt ttggaagaca
    19981 aggtatttga aaggtatatc gtgcttgatg attcggacgg ccctggcaca ctgtcggagg
    20041 tcgtggatgg aacagatatg agagtgatat gggggtccgg ccaccatgtt tcttaaagta
    20101 ttagggagca ttatcgtgct gctgtcgagc agctttctcg gatacataat gtcaagggac
    20161 tgcagcaaaa ggccgcagca gcttcgcagc ctgcagaacc tgctgcagat gttcgaaaac
    20221 cagatatctt attcatctga cgtactggcc gaagcgttcg aacggataag cagggctggc
    20281 ggcgagacag ggatcttttt caggaccacc gtggatctgc tgaaaaacgg cagtgctgca
    20341 aatgcgtcgg aagcatggga gaaggctgtt aagaagtgta tcggcaggac tgccctgaaa
    20401 agggaggatg aacagatact gctgtccttt ggaaagtcgc tgggaagcac tgatcttgag
    20461 gggcagatca aaaacatacg cctggcgctg ggacagcttg ccctgcaggt agaaaaggcg
    20521 gaagaaaaca ggaaaaagaa cgaatcgatg tataaaagcc ttgggatact cggagggttg
    20581 gccctggtca tcattcttat atagatttgt gttccgttgc tgcgctcata tccggaagag
    20641 gtctgtaata aagtaacaac aggaatgatc gacgaacaaa agaatcactt cgcaaaggag
    20701 ctgtgcagat ctatgggtat tgacttgatt ttcaggatag ctgcgatcgg gatcctggtg
    20761 gcggtgctca atcaactgct gatcaggtcg ggcagggaag aacaggcaat gatgaccacg
    20821 ctggcaggac tgattgtggt gcttttgata atcgtcaagg agatagcggt cctgttcgac
    20881 actgtaaaaa ccattttcag gctcgactga cctggcgaca tgcaggaacc ttgaaagcac
    20941 agagggataa ggtatggaga ttgttcagat catagggatc ggcctggttt ctactattat
    21001 tatgatcata ctgcggaagc agaagccgga gattgccgta ctggccggga tagctgcagg
    21061 agcgctcatg tttctgctta ttgccgccaa gttgtcagca gtggtcgacc ttcttgagga
    21121 atacgctgcc agggctgata tcaggccgat gtacttttct gccgtgctga agataacggg
    21181 aatagcatat attgccgaat ttggcgctga aatatgcagg gatgctggtg aaggagccat
    21241 agcggcgaaa atagagctgg tcggcaaggt gatcatagtt acgctggcaa taccgataat
    21301 cacttcttta ctcgaccttg tgctgaaaat aatgccataa cgggggataa agcaatatgg
    21361 tttacataag ggttatgaaa gtactgctgc cagtgttttt atcagtattg gcagcggtcc
    21421 tgctcatggc ggacgactgc cgggctgaaa gagatactga tgccatggac gccatgaaaa
    21481 ctgaaactgc cgatatactc ggagaacaga tcgaggcggg cgggataggc aggataggcc
    21541 atgaacttgg gaaatatacg gacgaagaaa cgatggagat gtttgaggga tttgatgctg
    21601 agcggctgat aagcgatctg gcggccggta cggcggatat ggatctgaaa ggaacaggcc
    21661 ggaaactgct gcgttttttt ttcagggagc tttaccagaa catcgggata ctggtgaaga
    21721 tcactgtgct ggtggtgatc tgtgcactgc tgaaaaacct ccagacaggt tttatgagtg
    21781 aaggggccgg gcagatagct ttttatgtct gctatatagt attggtgtcg atgctgcttg
    21841 taagtttcgg tacggtgatg aaaatgggca ttggcatcat cgaccgcatg atcggcttca
    21901 tgtatgcctc tgtacctgtt ctgatggcac tgcttatctc aggagggaat atcacagccg
    21961 gaggcatact gcatcctgtt atgctgctca tagttgagat ttccgccacg ctcataagga
    22021 acgtattcgt cccgcttgtt ttcatttcag cgatattgac aatcatcaac aacatatcgg
    22081 aaaaaatcca gcttacgagg cttgttaacc tgataaaaca gatagtgtct tggtctcttg
    22141 gcatcatact caccgttttt ataattgcca tctcgataca gggatccctg ggggcggtga
    22201 tagacggcgc tacccagaag gcggctaaat ttgccataag cacgttcata cccatagtcg
    22261 gcaagactct ctcggatgcc gcagacacag tcataggctg tacgctgatg ataaaaaacg
    22321 cagcggggat cggtgtaatg gcaggcatcc tgctcatatg cgtgctgccg ctgatcaaga
    22381 tcatcgccct cgcaggcttg ttcaaagcgg ctggggcatt gctcgagccg atatcggaga
    22441 gccgcatcac gaactgcata aatgacatag ccggttcgat gatccatata tttgcattgt
    22501 cagcttcggt ggcattcatg ttcgttgtga gcgttaccgc gctgataagc gcggggaaca
    22561 tcagcgccat gctcagatag gaaaagtttt cgggaggatg ggcggcaatg cttgagttca
    22621 taaaaaactg gactgccaat atcgttgttc tcgtactgtt tatcgtaatc actgaaatgc
    22681 tgctgccaaa gggcaaagtg aaaaaatatg caaacctgat gacaggcacc atactgatca
    22741 tcgccatcat cgaacctgtt accggcctgt tcggaaagtc ctttgatttc actttctccc
    22801 aggcagtgac ggcgggcagc atcgataaaa aagaaatcga aaaggcaggc aggatgtttg
    22861 aggaggagca ggtaaggcag accatcaggc tgtacaggaa caggataata gagcagatag
    22921 agcaccaggc gcgggaggtg gagggagtaa aggatgcgaa agctgatgtg atcataaacg
    22981 aggacagcag ttctccttct tttggagaga tcaagcgcat ttatatcgaa gcggaagtcg
    23041 aaggcggacc ggcgggacac aacagtacgg acaatcagga cggaagcggg gcgttcggcg
    23101 gcagtgtgcc tgcaaatgac ggagccccgg atgtggtcat ggttcccggt atccgggaag
    23161 tcgaacggat aaggatcgga ggcctggacg gaatgaagag aaaggcttca gttgaccgga
    23221 gactggagga acgactgtcg gaacgcattg caaatgcgtt cggtgtaaac cgtgaacata
    23281 taatcatcac acaggttgcg aggtgatccc atggaaactg tatataagat aatcggcagg
    23341 ataagggaat ggatagcccg acaggacagg aaaaaactag ctgagaatac ggccatcgtg
    23401 attatcattg gagtgatcat aatcatagcc ggatcagcaa tactggctcc ccggaagagc
    23461 aggcaggatg cccctgtaca ggaaacggcc cggagcggga cggcccatgc tgatatcgtg
    23521 caggacgcgg agaaagaagg agatatcgaa acacggctga aatcgatact gtcgcagatc
    23581 gagggagttg gaaaagtgga tgtcatgatc acatattcct ctacagcgga aaaggtaccg
    23641 gcttatgata tcaaaagaag ccaggatgaa accgaagaaa aggacagtgc gggcgggacc
    23701 aggaaaataa ggggagagga atatgagagt tccctggcat atgaggatat gtcttcagga
    23761 gggaaatcac ctgtgatact caaaaagctg gagccggaaa tacggggcgt gctggtggtg
    23821 gccgagggcg ctgagaatat cgaggtgaga gacaggatct gcagagctgt gacagtggtg
    23881 ctggacgtat ccgccaacag ggtccaggtt gtacagagga aaaaatgaag ttctctgaaa
    23941 gcatttcttt tgaacagatc cgttataaac gcaaaaagtg ctgaatatat tattaactga
    24001 gatggacaga tagagtgttc aaaatgtcgc aagtatcatg aagggggcat tgtgatgatg
    24061 atattgaaaa ggaagcagat agttatcctg gcgctgatac tgctgatcgt cgtagcgggc
    24121 tacctgcaat acagttacag gcagggaaat acgtcggctg attcggaagg caagcttgga
    24181 gaagcattgt atgtcgacaa tgagataata aatgaaggga tggccggatc ggtttccgac
    24241 ctggctgatg aaggttttgg cgaccaggta aatgtaacgg catcgaaaat ggcaaatgac
    24301 tacttcatcc aggctaaact agacaaagaa cagagcagga gcgaagcggc cgattcgctg
    24361 aaggcaatag cggaagatgc atcggtaagc cctgagataa gggaggaagc ccacagtaaa
    24421 atgatcgcgc tggcagagac cgtcgagaaa gaatcgagga tcgagatact gatcaacaag
    24481 atgggcttcg aagacagttt tatactgttt gccgataacg gaagcgtcga cataatcgtg
    24541 aagacgccca gccttaccag ttcccaggcg gcccagatca ccgacatagt gatgcgccag
    24601 gcagatgtag atgtcgagga tatccacata agcacgatgt tctgatgctg cggggcgatc
    24661 ctttgtcccc ggtcgattta ctttgacaat caaacgtgct ataatttaaa agcaaacaaa
    24721 catgtggagg caagcgatgg aagagaatgt acagagcgtg agcaatgata tagggactat
    24781 aaagataacc gatgaggttg tggcaataat agcagggatc gccgccatgg aagttcccgg
    24841 agtgacgtcc atgagcggag gtatagcagg aggcatagcg gaagccctgg gaaggaaaaa
    24901 cctgtccaag ggcgtaaagg tcgaggtggg agagaaggaa gctgcaatcg acctgtatat
    24961 aatcgtagaa tatgggtaca ggatacctga agtggcatgg acgatacagg agaaagtgaa
    25021 gaatgcggtc gaagatatga caggtctgaa ggttgtcgaa gtgaacatac atatccaggg
    25081 cgtcaatata gggagcgaac acaggaagga actgctggaa gatcaatcga agcaaaaatg
    25141 aacataatac cgtaaataaa cccgtcaggg tttatttttt ttggaaaata cactataata
    25201 aggtgtgggg acactcctca ggaaaacgga acggggacac tcttctacag ggaagcaact
    25261 acttagggct gaagaatgtc cccgtggctg gggttgagga atgtcgccca tttgataagg
    25321 tgtggggaca ctcctcaggg gaagatgtcg cccatttgat agacactccg ccgaaatcct
    25381 tgttccgaaa ggtgggttaa caaatgaaga tctttttcag gatacttttg gccatatatg
    25441 cgttctgcct ggctgtgttt tcagcatttg ccatgtatgt ggcgatccag ccggctgcat
    25501 atgtggatat ctcagagcaa ataatccagg ccatttcgac agataatgcc acggcgctca
    25561 ggatcgcggt attcgccgtc gcactggtat tttttgctct gagcattatg ttcctgctgt
    25621 ccggtgtcag aagcagcaag gataaaaaag ctgtcagcaa acatactagt atcggggaga
    25681 taaggatctc gctcaattct attgaaaata tcgcaataaa tgcatccaaa aagaccaatg
    25741 gagtcaggga ttcaaagacc atggtcagga aaacggatga tggggtggaa atagagatca
    25801 gagtggtagt gatgccggac atgagcattc ccgccataac cgaggatgta cagcagcggg
    25861 tgaaaaaatc tgtagaggat accgccggtg tcgctgtaaa aaccgtaaaa gtgatagtgg
    25921 acagtattta ttccggtatc acgtacaaac ccagggtgga ataaggagga aaaaccatgg
    25981 atacggaaaa gatcctggaa ttctataacg aacaccgtgg tgcgatcata ggggcggcag
    26041 caggtctgct gcttgcggtc ttcatattgc tgataggctt tttcagggtg ctctttattg
    26101 caatactgac aggagtgggg tactatatag ggaaaaagat gcatgaggat aaggattaca
    26161 taaagaacct ccttgacaga gtcctgccgc ccggcaccta tcgttgaagg agcagaattt
    26221 tttcagcagc cagaaggagg atggatgatg ggaagaagag cagccaggga aatggcaatg
    26281 aagctcttat accagatgga gatacagaag gatgacatag aatcccagat gaagctgttt
    26341 tttgaacata atcctgtttc agaaaacgat acgtcatata taagggacat aatttatggc
    26401 gtatatcgga attccgagta catcagcgat ataatacaga agcattccag gggctggaaa
    26461 ataaccagga tatcgaaaat cgacctgtcg atcctgaggc tgagcatata cgagatctgc
    26521 ttcagggatg acatcccttt cagcgtttcg gtcaatgaag ctgtcgagct cgcgaaaaaa
    26581 tacagcagtg aagaagcagg ctcattcata aacggcatac tcagcagatt gtcagattcc
    26641 aggacaggtg ccggaatcga aaacggttgt tgatatgatg acaagtacga atattttgac
    26701 tgtttccgaa gtgaataaat atataaaaga catgatatca agagacggta tcctgtcagg
    26761 cttgtgggtc aagggtgaga tatccaattt caagaaccat tattccggcc atttctattt
    26821 ttccctcaaa gacgagaaat cagtgctgaa atgcgtgatg ttcaggtcga acgcgtcatt
    26881 gctgccattt gtgccggaag acggcatgaa ggtcataatt agaggatatg tttccgtatt
    26941 cgaacgtgac ggacagtacc agctatatgc cgaagagata cagccggacg ggatcggtgc
    27001 tctttatatt gcttttgaga aacttaaaaa gaagcttgag gcagaagggc tgttcgatgc
    27061 agcgaagaaa aagaaactgc cgtatctgcc gaaggccata ggtgtggtca cttcgtcgac
    27121 cggagcggtg atcagggata taataaatgt gctgagccgg agattctata atttcaatct
    27181 gaagctctat ccggtccagg tccagggcga gcaggctgcg ggccagatag cggctgcagt
    27241 gcggagattc aatgaacttg acaacgtaga tgtgatcata gtggcaagag gcggaggatc
    27301 gctcgaggac ctgtggcctt tcaacgagga gatcgttgca agaagcatct atgaatcacg
    27361 gatcccggtt atttcagcag ttgggcatga aacggacttt accattgcag actttgttgc
    27421 cgatgtacgg gctcccacgc cgtctgcagc agcagaactc gtcatgcctg aaagggcagt
    27481 ggtcgagaac aggctggaca gcctgaaact tcgcctcaga aatgcggtta tgaaaaagac
    27541 ggccatggaa aggctgctgc tcagaaagat cgaaggcagc gttgcgttca ggcagcccct
    27601 gaacaagata taccaggaaa ggatgctgct tgatgtacag aaaaagtata tgcagaaagc
    27661 gttgtcagcg ctgaacacga attacaggaa caagctctcg ctgcttgctg cgacgcttga
    27721 cacgctgagc ccgctcaaat cactggccag gggttacagc ataacgaagt ctaaaaaaga
    27781 cggcagcctg gtaagatccg tacatgccgt cagtatcggt gacaggctgg aaatcaacct
    27841 tatggacgga aggcttggct gtactgtcga aagtatcgag gaggatagca atggataaca
    27901 acgaagtcac atttgaacag gcgatggcag agcttgagga gatagtaaag aagcttgaaa
    27961 agggagaact gacgctggac gaatcgatca acttcttccg caggggcgtc gaactgacca
    28021 agtactgcaa caggaaactc gatgaggctg aacgaagcat aacgatgctt atcgagggag
    28081 aaaacggcgt gatcgaaaaa gaactgcccg aacttgatca gggacggtaa accggctcgg
    28141 aacggaaggt gccaatgtac gggacaacag atttcaggaa aaaatacgaa gaatatgcca
    28201 atatcgtaaa caatgcgctc gaaaactgca ttgcaacaga tgacaaaccc ggatctgaga
    28261 tatacaatgc gatgaaatac agtcttgcgg caggagggaa aagactcagg ccggtgttgg
    28321 cgcttgcagt atgcgacatt cttgggggga acaggaacga catacttccc tttgcgtgcg
    28381 ctatagaaat gatacataca tattctctta tccacgatga cctgccggcg atggatgacg
    28441 atgattacag aagggggatt ccgtcgaacc acaaggtatt cggggaagca atggctatcc
    28501 tggcaggtga tgcgctgctc aataaggcat atgaaattat gatctcccat ataatgaaaa
    28561 aggaaggaga tctcaaggct tacgtacgcg caatggacgt gatcgctgtc agtgccggag
    28621 cagaaggcat g
//
LOCUS       DAOSCC010000006        25275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1414_length_25275_cov_5.132355, whole genome shotgun sequence.
ACCESSION   DAOSCC010000006 DAOSCC010000000
VERSION     DAOSCC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 25275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25275
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_25275_cov_5.132355"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLG72_00855"
     CDS             complement(<1..636)
                     /locus_tag="PLG72_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/uracil permease"
                     /protein_id="HOJ79384.1"
                     /translation="MDFLKDLGPFILAVAAVIINGVPQLLYAQARGFALKPAGFAYLV
                     GGFGNLLTGSVTPISSQAETITVASVKKNLRDNVSSILLASVLMITIAAFGGISKISD
                     FAGVAIISGMMSGVGLILAGVSLDMFNQDKRTSLVSIITAIAAYAVFYNSPNKVVWTI
                     FFSVAISTVDFLLLQKRRVDISATVEEGREYMKMSSEWRFWKKDYWKDFKLI"
     gene            883..1017
                     /locus_tag="PLG72_00860"
     CDS             883..1017
                     /locus_tag="PLG72_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79385.1"
                     /translation="MFCSTNKNNTLASQLKSETGDSFGVPEKNTRNSSQWTSPRANAP
                     "
     gene            1108..1476
                     /locus_tag="PLG72_00865"
     CDS             1108..1476
                     /locus_tag="PLG72_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79386.1"
                     /translation="MELSMISAIILVPLIMLLSGLQTVTKGSCTHTEGKSQQTGQNIA
                     DGIVFVKNAGHHARIGIIRSEKTSHDPGHHNTHTCRYGKTGGNSRQAYIAVHTLPFSL
                     FQFIHTPFNVGDTLISSSDR"
     gene            complement(1457..1924)
                     /locus_tag="PLG72_00870"
     CDS             complement(1457..1924)
                     /locus_tag="PLG72_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ79387.1"
                     /translation="MTLYINGYIHDIHDAEDLMIEAFSRMIHARPRLTPNGFRPYLYK
                     TARNLALRYAGKLCRRRDFSLEGLESEPESGILVEKVFQSGERNRILRECMDQLSPDY
                     CEVLYLLYYEGMTYAQAAQVMRKSTKQIDHLAERAKKRLRRMLEREGITDPNY"
     gene            complement(1884..2492)
                     /locus_tag="PLG72_00875"
     CDS             complement(1884..2492)
                     /locus_tag="PLG72_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79388.1"
                     /translation="MKFIALILILAIFLACLTSCFGMGIPKKPKDTNLEFWITQDVSE
                     TDFSGYCEILGWIGARQYYGSGYQPEVDENGNQTDPEYYVKYKVTAWPDYACQNKHIT
                     SIEITDPKVTLYGLTVDSSFDEFEQVFSEMGYEISRKSSGNYERIEASKKRITFSLES
                     NEGKREFTINAEITNVQGIAYSAGTPRGCIDALYQRLYTRYP"
     gene            complement(2529..3647)
                     /locus_tag="PLG72_00880"
     CDS             complement(2529..3647)
                     /locus_tag="PLG72_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HOJ79389.1"
                     /translation="MNIIDYAETQMERFESAGFNLVDSLVLSQFVYINLNGIVPGLTD
                     SGTPVRIGDLLKAEIIPHMLENVRDPKSNHRLLLALGMSPRFRDIRMYGFSDTLSITE
                     QKQFAAVTFLLDDKTAYIAYRGTDATIAGWKENFNMAFIYPVPAQQEGAAYMNAVAER
                     FPHHALMAGGHSKGGNIAVYSAMECDPAVQDRIVGIYSHDGPGFKDEIFTSEKYANIK
                     SKIHKTLPQSSLIGMLLQHHEDYVVVESRQFWIMQHDPFSWVVSNGDFQYRQGITGGA
                     KHIDTVINQWIASLDDEKRELFVTTIYKVIESMGVVKFSDFNGDWYKKAVSAMETIQG
                     IDTETRRFVLATIRSLLALYVKSLKLSIRDRGTLQQEQ"
     gene            3838..5067
                     /locus_tag="PLG72_00885"
     CDS             3838..5067
                     /locus_tag="PLG72_00885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOJ79390.1"
                     /translation="MKQERQYPKVMITPKAERSIKSGHPWIYGEEIRKTDGVPQNGGL
                     VDVFAGNAYMGTGFYNSASKITVRLISRNANDTFDARFWRRRVEYAVKYRKTVMPGPD
                     FACCRLVHGEADQMPGLTVDRYGGILSVQITCLGMELVKDMVYRALWDVLSEMGETVT
                     GIYERNDIDLRTREGLPEHKGWYTSDGISAPESAVTEIIENGVRYLVDVENGQKTGFF
                     LDQKYNRAAVARIARSKRVLDCFTHTGSFGLNAALGGAEHVTCVDISQPAIEMAKANA
                     VQNGLDDKMDFLCEDVFDLLTRLAEQKYRDYDLIILDPPAFTKSRRTVQSAARGYKEI
                     NMKAMKLLPRGGYLATCSCSHFMTDESFRKVLTSAAKDAAVSLRQIEARQQAPDHPIL
                     WNVPETDYLKFYIFQVV"
     gene            5248..5859
                     /gene="hisH"
                     /locus_tag="PLG72_00890"
     CDS             5248..5859
                     /gene="hisH"
                     /locus_tag="PLG72_00890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOJ79391.1"
                     /translation="MIAVVDYGVGNLFSLMSSLRFLGLDCIVTNKKEDLTGADRIILP
                     GVGAFGDAMAKLRESGLENTVKELAAVKPLLGICLGMQLLFDKSFEHGEHEGLGLIPG
                     VVAPLAADIDPALKVPHMGWNRLEITRDDPIFKYFDNGEWVYYVHSYYAKNCPESILA
                     VSEYGVMVTGLVRSGSGMIYGMQFHPEKSGDAGLRLLKAFSEI"
     gene            5910..6665
                     /gene="hisF"
                     /locus_tag="PLG72_00895"
     CDS             5910..6665
                     /gene="hisF"
                     /locus_tag="PLG72_00895"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ79392.1"
                     /translation="MISKRIIPCLDVMNGRVVKGVNFKDIRDMADPVEMARRYSEAGA
                     DELVFYDITASYEGRKLFTGVLRAVASEIFIPLTVGGGINSLDDFDMVLKSGADKVSV
                     NTGAIKDPSLISAAAKRYGSQCVVLSMDVKRVDGKFRVFANGGRIDTGIDALEWAKRG
                     EDLGAGEIVLNSIDTDGVRGGFDLPMLRAVGEIVAIPLIASGGAGKMEDFVELFRYDR
                     IDAGLAAGVFHTGKIEIGDLKSYLRECGVNVRL"
     gene            7019..7546
                     /locus_tag="PLG72_00900"
     CDS             7019..7546
                     /locus_tag="PLG72_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79393.1"
                     /translation="MLEEYVASYAASMFVLEDLTSEIDCCISDNRVDDRASSELARIR
                     KRIAVVEDRIASKLNSILNSPAWQGKLQDQVVSIRNGSYVIPVKREYRRSIEGTVVDT
                     SSSGSTVFIEPSAIRALKNELNLLRIDPPLLKPKFMRPYFAILNQSLIFLYMTDRMTK
                     EVNILCKYTKSELDY"
     gene            7620..8636
                     /locus_tag="PLG72_00905"
     CDS             7620..8636
                     /locus_tag="PLG72_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ79394.1"
                     /translation="MGTGTPLIQIRGLSKVFETESGEVNVLEDINLDIMEGEIFGIIG
                     MSGAGKSTLVRCLNFLEKPTKGTVYFDNQDLSALSQKELYRIRQSMGMIFQQFNLLMQ
                     RNVLKNVCFPLEIAGIRKNEAEARAYELLELVGLVDKAKAYPSQLSGGQKQRVAIARA
                     LANDPKVILCDEATSALDPATTKGILALLKDINKRLGITIVIITHEMSVVEEICQRVA
                     IIDSRHIAEVGYVQDIFTKPVTEAARRLVFPEGERVDHFTSSRCCRIVFNGSSSFEPV
                     IANMVLEFRQTVNIIFADTRNIDGKAFGQIVIQLPEDRLTGEKMMAYLRSRGLTVEEV
                     KGYV"
     gene            8629..9288
                     /locus_tag="PLG72_00910"
     CDS             8629..9288
                     /locus_tag="PLG72_00910"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOJ79395.1"
                     /translation="MFDQATWEMIGKGFLDSLYMTLVSSALAYLLGLPLGIILVVSDK
                     DGIKPCAWLNSVLGVIINLLRSVPFLILCITVIPVTRFITGTSLGPTATIVPLVIGAA
                     PFIARMVESSLKEVDKGVIEAAQSMGATPFEIIWKVLLPESRPSLILGAAISVTTILG
                     YSAMTGFVGGGGLGDIAIRYGYYRYQTDIMLVTVALLVIIVQVLQEIGLRLAKLGDKR
                     K"
     gene            9310..10122
                     /locus_tag="PLG72_00915"
     CDS             9310..10122
                     /locus_tag="PLG72_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ79396.1"
                     /translation="MKKTLSLLLVLTLAFTLLAGCGSKTSESKKIVVGATPAPHAEIL
                     EAAREVLKEKGYELEIREFTDYVQPNLSLQSGDLDANYFQHTPYLNDFNAEQKTDLVP
                     IASIHYEPLGIYPGKTKSLDDLKDGAQVAVPNDTTNEARALLLLEANGLLKLDPDAGL
                     KATPKDIVENPKNLKFVEIEAAQLVRSLEDVDIAVINGNYAIQGGLNAMTDALAKEEK
                     DSVAATTYANVLVVRKGDEDRKDLKALAEALQSEKVRKFIEEKYEGAVVPVF"
     gene            complement(10224..10862)
                     /locus_tag="PLG72_00920"
     CDS             complement(10224..10862)
                     /locus_tag="PLG72_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ79397.1"
                     /translation="MSMVKRVKCGNANVYIVSEGDSAILVDTGRKEYLDTVIDACKPC
                     KVKLIVLTHAHFDHAENAAELSERLEVPIAMHKDDVDLIESNNNQSMSARSFLGKIVL
                     SASLDGFTKREMRAFTPSVFLNDGDDLAEYGIAAKVIGLPGHTKGSIGIDVGEKELIA
                     GDALMNIFYPTLSMLYNDEEAMLESAKKITGLGERIIHFGHGRPVRNRIWVK"
     gene            complement(11023..11799)
                     /locus_tag="PLG72_00925"
     CDS             complement(11023..11799)
                     /locus_tag="PLG72_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ79398.1"
                     /translation="MANRIKTKKIDDCIWLMDDNGQATGYLVCGEKKAAVIDTMNGIE
                     DVHAVVREITDLPLVVVNTHGHCDHIFGNIYFDCDCYLHPDDMKVAAEHSNFPSFRKL
                     CSEKKLKMPPFKPINDGDVIDLGGLTLEAVSIPGHTPGGICVLLREKRVLFTGDSINH
                     HLWMQLDESLPLRKFLENLDRISWVTEKADRILHGHAQGFDDISLFDSLRSGVKELIE
                     TRGKGEKRQSTDYKWFRGKAKQFPFDDAGSVICYSDKKLD"
     gene            complement(11925..13766)
                     /gene="asnB"
                     /locus_tag="PLG72_00930"
     CDS             complement(11925..13766)
                     /gene="asnB"
                     /locus_tag="PLG72_00930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOJ79399.1"
                     /translation="MCGIAGWIDHNRDLRKDEEIINKMTATLVRRGPDAQGIYLKENV
                     CLMHRRLIVIDPENGKQPMTIEKGDASYTIVYNGELYNTKELRDELLASGYRFRGHSD
                     TEVLLTAYVHWGETCLERLNGIYAFGVWNDADRTLFLARDRMGVKPLFVYSYPGGIIF
                     GSELKTLLANPLVRPRVDNDGLKEIFLLGPGRTGGNGVIKGVTELRPAEFLVYEQKTG
                     SKIRKYWSLKACEHTDDVRTTIEKTRFLITDAVRRQLVSDVPLCCFLSGGLDSSIISK
                     IASDAYLSDNKGKLHTYSVNYVDNRQYFRKSVFQPNSDEEYTRMMSNYIGSQHHEVML
                     DNDDLEKYLGDAVLARDLPGMADVDSSLLLFCREIKKEFTVAVSGECADEIFGGYPWY
                     HNRDILCKDTFPWSHSLDLRQSILKPGLLKNGEEYVKQRYSDTIKAADTLPGEEEHEV
                     RMKQMFMLNLDWFMQTLLDRKDRMSMYSGLEVRVPFCDHRLVEYAYNMPWNIKSLNGR
                     EKGIVREAMKGILPEGIIQRKKSPYPKTHNPRYLEAVKSSLQSILDDPASPLVQLIDR
                     QKVQEIIDSDGRTLSAPWYGQLMTGPQLMAYLIQVDIWMREYKIGIE"
     gene            complement(14588..15172)
                     /locus_tag="PLG72_00935"
     CDS             complement(14588..15172)
                     /locus_tag="PLG72_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79400.1"
                     /translation="MKEGLLKEIAAHGDRGAIVPVSCFGELKREIEALRTDRHNMFIN
                     WMAGFMAITDEYGFQPRSLITVITPSPKIIVGFTYHGETIRCIVPPQYLDEGVKDNGI
                     LEYINACLKPFGYTAALFDRIPQKLLAVHSGLCLYGRNNICFHEEFGSYIRIISYISD
                     LPCDEADWFPARRMDACEKMPCLCGCLSDKSDRT"
     gene            complement(15368..16024)
                     /locus_tag="PLG72_00940"
     CDS             complement(15368..16024)
                     /locus_tag="PLG72_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ79401.1"
                     /translation="MKYRIAIFDMDGTILDTLEDLADSMNYCLKKYGMPERSLNEIKS
                     FVGNGIRRLVEQAVPDGADEGMIDKVYTTYIEYYKDHCAVKTRPYDGIREVLKKLKDK
                     GILTAVVSNKADLAVKKLCEEYFGGLFDYSVGDKDGMRRKPYPDSVISVMEYFKATPN
                     EVVYIGDSEVDYQTARNAGVDVIMVGWGFRDRDYLRSIGAERIIREPEEILEKMGIES
                     "
     gene            complement(16235..18190)
                     /locus_tag="PLG72_00945"
     CDS             complement(16235..18190)
                     /locus_tag="PLG72_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79402.1"
                     /translation="MISPVITARPLGEDITYFPPETQHLTVSVICEKGGGSSDDPYQV
                     YIRIKNTGSTPWKGVIRISLPVDAPAPRFFLPGFMYGTNRGETPLVVDSKCPRLREDG
                     EFPASPWWMVRSDRLSHPCAFAYGNGRLTGFAASPYYMRCGSRRVQWEPGIRGEFDQY
                     TGFGCSIQPGGVWYTLGYENAPWFFLDSHQYYPRAPLDKNCFCIEQGETVDVRIYCFD
                     IPGGDERVLHDALKWVYRKYHESPRRLCTVQETVRDMANAIARDAWLPEIHSYSCFVF
                     DKGDHFEYRLLPSIAWTNGLAVAVPMLASSLRLKDETIRQQALDCIDHIVRYSINEQN
                     DLPYLAEHDGKWSNRGWWYDRQRTPGHAAYLVGQSVYLALKAYALEKRERNIVHGDWL
                     DFAKRVIARTEKSRNADGEYPYILSEKTGAGLEYDSFSGAWCMAAAAYYTCLTGDRTY
                     LPSLLQSADYYHDAYIRHQECYGGPLDIDKNIDSEGVLAYIRAVRHLHEITGEEHLLM
                     YLRDALYYEYTFKFCYNSPIKIPPLSTAGWSSCGGSITSVVNPHIHPMSSSVMDEMVY
                     FLSRQDDGYIRSRLEDTLLWSCQCHMIADREYGYGRKGWMSERFCHSEGLLTERYPDG
                     TPASTWFALMPWACGSILEGLTGELWP"
     gene            complement(18243..19097)
                     /locus_tag="PLG72_00950"
     CDS             complement(18243..19097)
                     /locus_tag="PLG72_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79403.1"
                     /translation="MAVYEYLLKEKIQYIKIDEKYELPFSDETKDLIVEMWSAYRNGK
                     FFNSENVRLDAVRTEGDKSVLVISRTDFYSLLLTNIIRLDISGFRVFAEEHCKNDKYL
                     KSLQELLEYYSSLKECNGFEDLILNGNLANALAISVQVVDTYGNAMLVKRSGNVGIGK
                     NLYSVTSTGAVDLIDWNTDDPIRSCAVRELEEELNLRLDCSDLEITGIVAGVVKKQPI
                     AILNAYVDHDLREINGESARADDYKFEVDKVHICRIDEIESILDSQEFTEAAEYHLKK
                     IINDSKLG"
     gene            complement(19084..19899)
                     /locus_tag="PLG72_00955"
     CDS             complement(19084..19899)
                     /locus_tag="PLG72_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ79404.1"
                     /translation="MSSYSERGDGPDNEDAYGYRDNIFWVIDGATDLYHLNLFGCDDD
                     VARYVRQLNQCIYDNANGKDRLADILRKSVSDTNKLLSLKTEDYEIYKLPSFAVLLAR
                     MTGSKCEYYILGDCVLLVKNKGEIIKLTDSRLLVFTEKNRSGIEALKKSGKLTYENKI
                     ELLRDTRKYMNTENGYWIGSVDCAGIDNAIQGEIDIDPETMCLGCSDGLMEAFDLFGI
                     SAIDGSIFDAEKLKHIVNALRKCQQDDARNSVARVRKKDDLTYILVRCAYGSI"
     gene            complement(19918..20622)
                     /locus_tag="PLG72_00960"
     CDS             complement(19918..20622)
                     /locus_tag="PLG72_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ79405.1"
                     /translation="MKNIIVTGGSGTIGTAIAEVLLRNGYKVYVVDVKEPEKKANQVF
                     IHCDVTKYDDLTKAFRKEFDGSAVDGIVTVAGGVLPEEWSDFSDTDMEVIRKSIELNL
                     VGHINAVHAVLPYLIASKEDKSITMISSINGMAAYRMAGYSASKAGLAGFMYGIAKEM
                     GEKGIRVNVVSPGTVVTELTLRESKKEWSKLREGTLTGQFAVPEDIAQLVYMLICNKS
                     IAGQNIVIDSGQLIKK"
     gene            complement(20663..22093)
                     /locus_tag="PLG72_00965"
     CDS             complement(20663..22093)
                     /locus_tag="PLG72_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79406.1"
                     /translation="MDNAVVVLDDVQKLIDEYIAYPNSEDGLKKLVRFITQIFISEDK
                     GFEVSNDDGKLQEIAASVCKLNETDIEKAYHYTVRKVNDPELEIYWLLRYLPDDKTTA
                     FTRKINKYLKNKRALDSKAFIDFIEDRNEDVMLRFKAFYILFTYHHDQKDISKNKRIV
                     ETYRDDFSIFPLWYYTNSQILYQEERNKNKADMSEALASAKKCIDIYNTDKRYDPNYP
                     GIYHNFCELVFYASELGDKESFEDNYELAGEYIEKAKSLNPKFAKYYYTEGRLLMAKC
                     RYVSPEEAGRLYDKAEKLFNRAIDMEDSSREDYAIKIVDYETALLRCKTERRLKEIDV
                     ALKEAKDNQEKTVEIQKQTQALVQDSKTLKEELEEQKKGTLELLGFFSGIISLIVVTS
                     QVVLNLDMISACVVMLLFLGTLIMAFSFFHFIILNGARKTIQSSEKDNARENARASAN
                     TRAMVLFMLFAVILIIGAIVIAAVIG"
     gene            complement(22396..23502)
                     /locus_tag="PLG72_00970"
     CDS             complement(22396..23502)
                     /locus_tag="PLG72_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79407.1"
                     /translation="MSRSFIDEFYWTDFKNQKDESLSGTEKYQRIVNWAVKARVHNPN
                     VFDALTEWILDDSEWTDDKLAENEQILMQGILARFRSQNAQLRAYLKDIEPGIVNEAV
                     FNALDRFDDELSGKTNENHLRDVVSKMFTDFSVMRDRKIREQIAGGKTGPEGVDSVDV
                     FGYVNLLKTCDARVQWTLFMPGAVEKQLEGFKVEKFEYIKMPALRFIGKESRESDGRD
                     TVEDLKKIFGVLDTMSEYKSGFDYDVLLLHHYGRGVDAEPCHGFWGRFMKADTPVPEG
                     FVYFDFTPQRAEKGFAAGLPYISQFAFAVFSGDMEAMHKNEGYECGAMYDITRNIILG
                     QDVNIPYPDKYWTAEVFLNGYDNYSTAYMFSVEL"
BASE COUNT         6333 a         6478 c         5597 g         6867 t
ORIGIN      
        1 tatgagtttg aaatccttcc agtaatcctt tttccagaag cgccactcgc tgctcatctt
       61 catgtattcc cgtccctctt cgactgttgc ggatatatcg accctgcgct tttgcaggag
      121 caggaagtcg acagtggata tcgcgaccga gaagaaaatc gtccatacga ccttattggg
      181 gctgttgtag aaaacagcgt atgcagcaat agctgtgata atggaaacca gcgatgtacg
      241 cttgtcctgg ttgaacatat ccagcgacac accagccagg ataagtccta cgccgctcat
      301 catacccgaa attatcgcca cgccggcaaa atcggagatc ttgctgattc cgccaaaagc
      361 agcgatagtg atcatcagta ccgatgcgag aagtatggaa ctgacgttat cccttaggtt
      421 cttctttacg ctggctactg tgatagtctc agcctgggaa gaaatcgggg tcaccgaacc
      481 ggtaagcaaa ttgccgaaac ctcctaccag gtaggcaaaa cctgccggtt taagtgcaaa
      541 accgcgggcc tgtgcataaa gaagctgagg cacgccatta atgataactg ctgcgactgc
      601 tagaataaat gggcctagat ctttcaagaa atccatggtt ttcacccctg tttgcgtttt
      661 tatgtaaaca atatatctca ttcggacatg tacgacaaat cagttatact aatgctaact
      721 attagttata gtaatggttt catctcattt gcaatattca gagcgaagac tgacatatcg
      781 tttgcgattt gaccgggaac ggagttacta taagtaatac aaaccaatta gctttatttt
      841 tccattcata atcgactctc attatgcagc tgcaggtaca gggtgttttg cagtacaaac
      901 aaaaacaaca cgcttgccag ccagcttaaa tctgagacag gagacagttt cggagtccct
      961 gaaaaaaaca ccaggaacag cagccagtgg acaagcccga gggcaaatgc cccgtagaag
     1021 atggtaagca aaaaccaggc ctgcatttta tgccgtatcg acatcacggc ggagtaaata
     1081 cagcaccctg caacgaccgc gagctgaatg gaattgtcaa tgatttccgc cattattttg
     1141 gttcccctta tcatgctgct ttccggactt caaacggtaa caaagggcag ttgcacacac
     1201 accgagggaa aaagccagca aaccggtcaa aacatagccg atggcattgt tttcgtaaaa
     1261 aatgctggcc atcatgctcg tattggcata atccgttccg aaaagaccag ccatgacccg
     1321 gggcatcaca atacccacac ctgccgttac ggcaagacag gcggcaacag ccgacaggcc
     1381 tatatagctg tgcatacgct gcctttttct ctgtttcagt tcattcacac gcctttcaat
     1441 gtcggcgata cgctcatcag tagttcggat cggtgatccc ctcccgttcc agcattcggc
     1501 gcagtctttt tttcgcgcgc tcagccagat gatcgatctg cttcgtgctt tttctcatga
     1561 cctgcgccgc ctgggcatag gtcattcctt cataataaag caaatacagc acctcgcaat
     1621 aatcggggct gagctggtcc atgcattccc gcaggatgcg gtttcgctcc ccggactgga
     1681 acactttttc caccaggatc ccgctttcag gctcgctttc cagtccttca aggctgaaat
     1741 cacggcggcg gcaaagcttt cccgcatacc gcagtgcaag attccgtgcg gtcttataaa
     1801 gatatggcct gaatccattt ggggtcagcc gcggcctggc gtgaatcatg cgtgaaaaag
     1861 cttcgatcat cagatcttca gcatcatgga tatcgtgtat ataaccgttg atatagagcg
     1921 tcaatgcatc cccgtggcgt tccagcagaa tacgcgatcc cctgaacgtt tgttatctct
     1981 gcatttatag taaattcacg cttcccttca ttagattcaa ggctaaaagt gatgcgcttt
     2041 ttcgaggcct cgatgcgctc atagtttccg gaactctttc gggaaatctc atatcccatt
     2101 tcactgaata cctgctcgaa ctcatcgaat gatgaatcaa cagtcagccc gtataacgta
     2161 actttcgggt cagtgatttc gatcgaagtt atatgcttgt tttggcaggc atagtccggc
     2221 cacgcagtga ccttatactt gacataatac tccggatcgg tctgattccc attttcatcg
     2281 acctcgggct gatagccgga gccatagtat tgcctggcgc cgatccatcc aaggatctca
     2341 cagtatccgg aaaaatcagt ttctgaaaca tcctgcgtta tccagaactc aaggttcgtg
     2401 tctttcggct ttttgggaat acccatgcca aaacacgatg tgaggcaggc aagaaatatt
     2461 gccaatatga gaatgagagc aataaatttc atagttcacc accctcgagt tttttcgtaa
     2521 atatgaattt actgttcctg ctgcaatgtc cctctgtccc tgatggagag cttgaggctc
     2581 ttcacataca aagccagaag cgatcttatt gttgcaagaa cgaagcgcct tgtttcagta
     2641 tcgatcccct gtatggtttc catggcgcta acagcttttt tgtaccagtc cccgttgaag
     2701 tcactgaatt tcacgacgcc catggattca atgactttat atatcgttgt cacgaacaat
     2761 tccctcttct catcatcaag ggacgcgatc cactggttga tcaccgtatc tatatgcttt
     2821 gctcctccgg ttatgccctg cctgtattgg aagtctccat tgctgaccac ccatgagaag
     2881 ggatcgtgct gcattatcca aaactgcctg ctttccacga caacataatc ttcgtgatgc
     2941 tgcaggagca tccctatcag cgaagactgc ggcagtgttt tatgtatctt cgatttgata
     3001 ttcgcatatt tctcgcttgt gaatatttca tccttgaaac cggggccgtc atggctgtag
     3061 atgccgacaa tgcggtcctg gaccgcagga tcgcactcca tggcggaata gactgcgatg
     3121 tttcctccct tggaatgtcc cccggccatc agcgcatgat gtggaaaccg ttctgccact
     3181 gcattcatat aggcagctcc ctcctgctgt gcaggcaccg ggtagataaa cgccatattg
     3241 aaattctctt tccagccggc gatggtagca tctgtacccc gataggcaat atatgcggtt
     3301 ttgtcgtcca gcaggaaggt aacggcggca aactgcttct gctcagttat gctcaaggtg
     3361 tctgaaaagc cgtacatccg gatatcccga aaccttgggc tcatcccgag ggcaagcaga
     3421 agcctgtgat tgctcttcgg atcccgtacg ttttcaagca tatgcgggat gatttccgct
     3481 ttcagcaggt ccccgatcct gacaggagtc ccgctgtccg taagtcccgg cacgatgccg
     3541 ttgaggttga tatagacgaa ctgggacaag acaaggctgt ccaccaggtt gaaaccggcg
     3601 ctctcaaacc tctccatctg tgtttcggca taatctatga tattcatgtt tatttccttt
     3661 cagatctgtc ccgatatatc ggcactgttc cggaaatact gcttttttta ctgtttacat
     3721 gtgaatattt tactggattc tttgtacaat aacaagacag cgcagaccat gttgaaggcg
     3781 gaagaagaat atggtaaaat cagcgcgttg atattcttat ttgttaaggt ggcacagatg
     3841 aagcaggaac ggcagtaccc aaaagtcatg ataactccaa aggcggagcg cagtataaaa
     3901 agcggccatc cgtggatata cggggaagag atccgcaaaa cggatggcgt gcctcagaac
     3961 ggcgggctgg tggatgtgtt tgccggaaac gcctatatgg gtacgggctt ttacaacagt
     4021 gccagcaaga tcacagtccg gctcatttca cgcaacgcca acgatacgtt tgacgcccgc
     4081 ttctggcgcc gccgcgtgga gtatgccgtc aaataccgta aaaccgtcat gcctggcccg
     4141 gactttgcct gctgccgcct ggtccacggc gaggccgacc agatgccggg gctcacagtg
     4201 gaccgttatg gcggtatcct gtccgtgcag atcacctgcc tcggcatgga acttgtcaag
     4261 gatatggttt accgtgcact ttgggacgtg ctttccgaaa tgggcgaaac cgtcaccggc
     4321 atttatgagc gcaatgacat agacctgcgt acacgcgaag gattaccgga acataaaggc
     4381 tggtatacgt ccgatggcat atctgcaccg gaatcagcag tgacggagat aatcgaaaat
     4441 ggcgtgagat acctggtgga tgtggaaaac gggcagaaaa ccggcttttt cctcgaccag
     4501 aaatataacc gtgccgctgt tgcccggatc gcaaggagca agcgggtgct ggactgcttt
     4561 acgcatacag gctcctttgg cctcaacgct gcgctgggcg gggcagaaca tgtcacctgc
     4621 gtggatatct cacagccagc catcgaaatg gcaaaagcaa acgccgtgca gaacggtctg
     4681 gacgataaga tggacttcct gtgcgaagac gtgtttgacc tgctgacaag gctggcagag
     4741 caaaaataca gggattatga tcttataatc ctggatccgc cggcctttac caagtcacgc
     4801 aggactgtgc agtctgccgc ccggggatat aaggagatca acatgaaagc catgaaactg
     4861 ctgcctcgcg gcggatacct tgccacctgc agctgcagcc acttcatgac tgatgaatca
     4921 ttccgcaaag tgctgaccag cgctgcgaag gacgccgccg tatctctcag gcagatcgag
     4981 gcccgtcagc aggccccgga ccatcccatc ctgtggaatg ttccggagac ggattacctc
     5041 aagttttata ttttccaggt cgtatgatct gaaagcagta tgacctggga gcaatttagc
     5101 ctgatgcctg agtttcaggt ttggcggaaa tttaccgggg gaactgcaca cagaagggtt
     5161 ccgtagctgt tgttgaagct gttcagatta aaactgttca gatatttgcc aggtcggtct
     5221 cgacccatga agggggtgct tatataaatg atcgctgttg ttgattatgg tgtcggcaac
     5281 ctgttttcgc tgatgtcaag cctgcgcttt ttagggctcg actgcatagt gacaaataaa
     5341 aaggaagatc tgacgggagc agacaggatc atccttcccg gcgtcggcgc tttcggcgac
     5401 gccatggcaa agctcaggga atccgggctt gaaaacacgg ttaaagagtt ggcggcagtt
     5461 aaaccgcttc tcggaatatg tttgggaatg cagcttttgt ttgacaaaag cttcgaacac
     5521 ggtgagcatg aaggcctggg gctcattccc ggagtggtcg cgccgctcgc ggccgatatc
     5581 gacccggctt tgaaagttcc gcacatgggc tggaacaggc ttgagatcac cagggatgac
     5641 ccgatattca aatacttcga taatggtgaa tgggtctact atgtccacag ctattacgct
     5701 aaaaactgcc cggaaagcat actggccgtt tcggaatatg gcgtcatggt gacggggctc
     5761 gtgcgttccg gttccggcat gatctacggt atgcagttcc atcccgaaaa aagcggtgat
     5821 gcagggcttc gtttgctcaa agcattttcg gaaatatgac ggatgacggg taatatcgtt
     5881 agtaattcta tatgttcgga ggcaatccga tgatcagcaa aaggataata ccatgccttg
     5941 atgtgatgaa cggaagggtc gtcaagggcg taaacttcaa agatatccgg gatatggcgg
     6001 acccggtgga aatggcgcga cggtacagcg aagctggcgc agacgaactt gtattttacg
     6061 atatcacagc aagttatgag ggaaggaagc tctttaccgg cgttttgcgg gcagtggctt
     6121 ctgaaatctt cattcctctc actgtaggag gcggtataaa ctcgctcgac gattttgata
     6181 tggtgttgaa gtccggtgct gacaaggtaa gcgtcaacac cggcgccata aaggatcctt
     6241 cgctgataag cgctgcggca aagcgctacg gcagccagtg cgtcgtactg agcatggacg
     6301 ttaaacgcgt ggacggtaaa ttccgtgtct tcgcgaatgg cggcaggatc gacacaggaa
     6361 tcgatgccct cgaatgggct aagagaggag aagatcttgg agccggagaa atcgtgctca
     6421 actccataga caccgacggc gtgcgcggag gctttgacct cccgatgctc cgcgcggtag
     6481 gtgaaatagt tgcaataccg cttattgcaa gcggaggggc gggcaaaatg gaagatttcg
     6541 ttgaactgtt caggtatgac aggatagatg caggtcttgc ggcaggcgta ttccatacag
     6601 ggaaaataga aataggggac ctgaaaagtt acctcaggga atgcggcgta aacgtacgcc
     6661 tgtaaaagac aatagcaaat ttgattatag gctgtatcag gatttttgct tttttcattt
     6721 gttgtttaag taattccaca gtatttgaag acgggccttt gcatcaataa aattatcaga
     6781 gcaaaaccct cgcatccatt ctctttcttt atcaaatggg tcaatccaga aacgcttcat
     6841 aaatttcctc aacattgatc ggatatccca agccaggcct gaaactgaac agacaagttg
     6901 aatgaattgg aaaatacgat cctttcataa tccttgatca tcgcaaaacc tggaaaagct
     6961 tctataccaa ttgggtatct gattgttctg tatcccaaaa taccagaaga tcaaggaaat
     7021 gctggaagaa tatgttgcgt cctacgccgc ttccatgttt gtcctggagg atctgacttc
     7081 cgagatcgac tgctgcatca gtgataacag ggtcgatgac agggcgtctt cggaactggc
     7141 gcgtatcagg aaacgaatag ccgtcgtcga ggaccggatc gcgagcaaac ttaacagtat
     7201 cctgaactcg ccggcctggc agggcaaact gcaggaccag gtggtcagca tccggaacgg
     7261 aagctatgtc atacctgtca aacgcgagta caggcgctct atagagggca cggtcgtgga
     7321 tacctcatcc agcggttcca ccgtgttcat agaaccctca gccatccgcg ccctgaaaaa
     7381 tgagctgaat ttattgagga tagatcctcc gcttttgaaa ccgaagttta tgcgtccata
     7441 ttttgccatt ctcaatcaaa gcttgatttt tttatatatg acagatagaa tgaccaaaga
     7501 ggttaatatt ttatgcaaat acacaaaatc tgagctggat tattgacaaa gtcattcggc
     7561 cagataaaat ataattaaac ctatatgaat aatatgataa cagataggag tcacattcaa
     7621 tgggtaccgg tactcctctc attcaaatca gaggactcag taaggtcttt gagaccgaat
     7681 caggtgaggt caatgtcctt gaggatatta accttgacat catggaaggc gaaatatttg
     7741 ggatcattgg tatgagcggc gccggcaaga gcacactggt gaggtgcctg aactttcttg
     7801 agaaacccac aaaaggcacc gtgtattttg acaaccagga cctgtccgcc ctcagccaga
     7861 aggaacttta caggatcagg cagtccatgg ggatgatctt tcagcagttc aacctgctca
     7921 tgcagcgaaa tgtcctgaaa aatgtgtgct ttcccctgga aatcgccggg atcaggaaaa
     7981 acgaggctga agcccgagca tatgaactgc tggaactggt aggcctggtt gataaggcca
     8041 aagcttaccc gtcacagctt tcaggggggc aaaaacagcg tgttgccatt gccagggccc
     8101 tggccaacga tccaaaggtc atcctgtgcg acgaggcgac cagcgccctc gacccggcaa
     8161 cgaccaaggg gatccttgcc ctgctcaagg acataaacaa aaggcttggg atcaccatcg
     8221 tcatcatcac ccatgaaatg agcgtggtgg aagagatctg ccagagggtg gccataatcg
     8281 acagccggca tatcgctgaa gtaggctatg tacaggacat ttttacaaaa cccgtgacag
     8341 aggctgcccg ccggcttgta tttcccgaag gggaaagggt ggatcacttc accagttcac
     8401 gatgttgccg catcgtgttc aacgggagtt cgtcctttga gcctgtcatc gcgaatatgg
     8461 tgcttgaatt caggcagacc gtcaacatca tttttgccga caccaggaac atcgacggga
     8521 aagctttcgg gcagatcgtc atccagcttc cagaagacag gctgacagga gaaaaaatga
     8581 tggcttacct gcgcagcagg ggactaacgg tagaggaggt aaagggctat gtttgatcag
     8641 gcgacttggg aaatgatcgg gaaaggattc ctggattcac tatatatgac gctggtgtcc
     8701 tcggccctgg cctatctgct gggactgccc ctgggcatca tactggtggt ctccgacaag
     8761 gacggcataa agccatgtgc atggctgaac agcgtcctgg gcgtaatcat caacctgctt
     8821 cgttcagtgc ctttcctcat tctctgtatc acggtcattc ccgtgacacg gttcattacg
     8881 ggaacttccc tgggtcctac cgccaccatc gttccgctgg tgatcggcgc ggctcctttc
     8941 attgcaagaa tggtggaatc ctcgctcaag gaagttgata aaggcgtgat cgaagccgcc
     9001 cagtccatgg gagccactcc ttttgaaatc atctggaagg tactgcttcc cgaatcgcgc
     9061 ccgtcactca tacttggcgc cgccatttct gtgacgacca ttttgggcta ttcggcaatg
     9121 accgggttcg taggcggcgg aggtctgggc gacatagcca tccgttatgg ctactacagg
     9181 tatcagaccg atataatgct ggtcactgtc gctctcctgg tcataatcgt ccaggttctc
     9241 caggagatcg gcctgagact ggccaagtta ggcgataaaa gaaaataggt aataaagggg
     9301 gaaaatataa tgaagaagac attatctctg ttacttgtgc ttactttagc gttcacactg
     9361 cttgcaggct gcggcagcaa aacttcggaa tccaaaaaga tcgtcgtggg agcgactccg
     9421 gcgccccatg ccgagatcct ggaagcagcc agagaagttt tgaaggagaa gggttatgaa
     9481 ctggagatca gggagttcac cgattatgtc cagcccaacc tttcactcca gagcggcgac
     9541 cttgatgcca actacttcca gcatactcct tatctcaatg acttcaatgc cgagcaaaaa
     9601 acagaccttg tacctatcgc aagtatccat tatgaaccgc tgggtatcta ccccggcaaa
     9661 acaaaaagcc ttgacgacct gaaagacggc gcacaggtgg ccgttcccaa tgataccacc
     9721 aatgaagcga gggcgctgct gctcctggaa gccaacggcc tgttaaagct tgatcccgat
     9781 gcagggctta aagcgacccc gaaggatatc gttgagaatc ctaaaaacct taagtttgtc
     9841 gaaatagaag cagctcagct ggtacgttca ctggaagacg tagacatagc tgtcatcaac
     9901 ggcaactatg cgatccaggg cggcctgaac gcaatgaccg acgccctggc aaaggaagaa
     9961 aaggattccg ttgcagccac cacttacgcc aatgttcttg tggtacgtaa gggcgacgag
    10021 gacaggaagg atctgaaagc ccttgccgaa gcccttcaga gcgaaaaagt caggaagttc
    10081 atcgaggaaa agtatgaagg cgcggtagtt cccgtgttct gatcaaccgg cttcagagta
    10141 acaaatgcat gaaaactccc gggaatgtga ttccccggga gttttttatg ggcacggtcc
    10201 cctgtctttt cattcggctg tcgttatttc acccatatcc tgtttcgcac aggcctccca
    10261 tggccgaagt gaatgatgcg ttctccaagc ccggttatct tttttgcgct ttcaagcatg
    10321 gcttcctcat cgttgtacag catggacaag gtcgggtaaa atatgttcat cagcgcatcg
    10381 cccgctatca gctctttctc tccgacatct atgcctatgg agcctttggt gtgtcccgga
    10441 aggccgatga cttttgccgc gattccatat tctgctaaat catctccgtc attcagaaag
    10501 acagacggag tgaatgctct catttccctt tttgtaaagc cgtcaagaga agcggataat
    10561 acgatcttcc ccaggaaaga ccttgctgac atggattgat tattattcga ctctatcagg
    10621 tcaacatcat ctttatgcat cgcaatggga acttcgagtc tttctgataa ctcggcagca
    10681 ttttccgcat gatcgaaatg cgcatgggtc aatacgatca gtttcacttt gcagggcttg
    10741 caagcatcaa tgacagtgtc aaggtattcc ttcctccctg tatctacaag tattgccgaa
    10801 tccccttcgg aaactatgta gacgttcgca ttgccgcact ttaccctttt caccatactc
    10861 atgccgctct ctctcttaat gtgctttttt atcgtccttt tttatgattc cgtggatgat
    10921 agtgtcgagt atctcatcat taactcgtct tttggccagg gatagcttcc gcataaatct
    10981 ccattatgcc ggcaaactgt aataagtaat acatagctgg actcaatcca gcttcttgtc
    11041 gctgtagcag ataacgctgc cggcatcatc gaaaggaaat tgctttgctt taccgcggaa
    11101 ccatttataa tctgtgcttt gccttttttc acctttccct ctcgtctcga tgagttcctt
    11161 tacgccggac cgcaggctgt caaacagtga tatatcatca aagccctgcg cgtggccgtg
    11221 caggatcctg tccgcctttt cggtcaccca tgagatcctg tcgagatttt caaggaattt
    11281 cctgagcggc aggctttcgt ccaactgcat ccaaagatga tgattgatgc tgtcgccggt
    11341 aaacaatacg cgcttttctc ttaaaagcac gcatatccct ccgggggtgt gcccgggaat
    11401 cgaaacggct tccagcgtaa gtccgccgag gtcgatcaca tcgccgtcgt tgattggttt
    11461 gaacggcggc attttaagct tcttttcact gcagagcttt ctgaatgagg gaaagtttga
    11521 atgctctgcg gcaactttca tgtcatcggg atgcaaatag caatcacagt caaagtatat
    11581 attgccgaag atatggtcgc agtggccgtg ggtatttaca accactaaag gaagatcggt
    11641 gatctccctt acgaccgcat ggacatcctc gataccgttc attgtatcaa taaccgctgc
    11701 ttttttctcc ccgcagacaa gataccctgt cgcctggccg ttatcatcca taagccatat
    11761 acagtcatcg atctttttgg ttttgatcct gttcgccacg cacaacaccc cttatctttt
    11821 tttgtttttt ccatgagttt ctccagcaac agcgaaatac tgacctttat tattcgccat
    11881 gtactcattt tacctctccg ggtacgatca ggcaattgaa acttttactc gatcccaatc
    11941 ttatattccc tcatccatat atccacctgg atcagataag ccatcagctg cgggcctgtc
    12001 atgagctgcc cgtaccatgg cgcggaaagg gttctcccgt cggaatcgat gatctcctgt
    12061 accttttgcc tgtcgatcag ctgcaccagt ggagaggcgg gatcatccag tatgctttgc
    12121 aggcttgatt tgactgcttc caggtacctg ggattgtggg tcttcggata agggcttttt
    12181 ttgcgctgta tgatcccttc aggcaaaata cccttcatgg cttccctgac gatacctttt
    12241 tcccttccgt tgagcgactt gatgttccag ggcatgttat aggcatactc caccaggcgg
    12301 tggtcgcaaa agggcaccct tacctcaagc ccgctgtaca tgctcatcct gtctttcctg
    12361 tccagcaaag tctgcatgaa ccagtcaagg ttcagcatga acatctgctt catgcgtact
    12421 tcatgctctt cctcgcccgg cagtgtatcc gctgctttta tggtatcgct gtaccgctgt
    12481 ttcacatact cttccccatt cttcagcaag cccggcttca ggatgctttg ccttagatcc
    12541 agcgaatgcg accatgggaa ggtgtctttg cataatatgt ctctgttgtg ataccacgga
    12601 tagccgccga atatctcatc cgcacattcg ccggaaaccg caacggtgaa ctcctttttg
    12661 atttccctgc agaagagcag cagtgaagaa tcgacatccg ccataccggg cagatcacgg
    12721 gcaagcaccg catcgccaag atatttttcc aagtcatcgt tatccagcat aacttcatgg
    12781 tgctgtgaac ctatgtagtt tgacatcatc ctggtatatt cctcgtcgga gttgggctga
    12841 aaaacacttt ttctgaaata ttgccgattg tcgacatagt tgaccgaata tgtatggagt
    12901 ttccctttat tgtcggacaa atacgcatcg gatgctattt ttgaaataat actggaatca
    12961 agtccgccgg acaggaagca gcacaacggc acatccgaca ccaactggcg cctgacagcg
    13021 tctgttatca ggaaacgggt cttctcaatg gtggtcctga catcgtctgt atgttcacag
    13081 gccttcaggc tccaatattt ccggatcttc gaccctgttt tttgctcata gaccaggaac
    13141 tctgccggcc tcaattcggt tacgccctta atgacgccat tgccgcccgt cctgcccggg
    13201 ccgaggagaa aaatctcttt aaggccgtca ttgtcgaccc tgggcctcac cagggggttt
    13261 gccagcaatg ttttcaactc tgaaccaaat atgataccgc caggataact gtagacaaac
    13321 agcggcttta cgcccatcct gtcgcgggca aggaacaaag tccggtcagc gtcgttccat
    13381 actccgaagg catatatgcc gttcagcctt tccaggcacg tttcgcccca atgtacgtac
    13441 gccgtcagca ggacctccgt atcggaatgc ccccggaagc gatatccgga agcaagaagc
    13501 tcgtcgcgga gttcctttgt gttataaagc tctccattat acacaatggt atagctggca
    13561 tcgccttttt cgatcgtcat cggctgctta ccgttttcag ggtcgatcac gatgagcctc
    13621 cggtgcatga ggcatacgtt ttccttaaga tagatgccct gcgcgtcagg cccccttcgt
    13681 accagcgttg ccgtcatttt atttatgatt tcttcatctt ttctcaagtc cctgttgtga
    13741 tctatccatc ctgcgatccc gcacataaac accctcctga aataaataaa ggcgccactg
    13801 tcagtgacgc ctttgtcatc gctatatatt tatgttatat gcagtgcccc gggaattgtt
    13861 gcttatcatc cgcagtccgt catatccggc cggtatccgc tatattcgat cgctgcggcg
    13921 ttgccatgcc attccggaaa ctgacgaata tccgacgcag ccggccacgc ggccgttgtc
    13981 atcagtttca gcttgcgtct acagcaatga acggtataaa aagtcaaagg ccctgtccag
    14041 aaacaaatca gcgttacatt ctctgaagaa gagacgatag agctgctgat gcataaagat
    14101 gctgaaccgt attctgagcc gcttgccgcg aagataaggc agtcgggttt caatgcaggt
    14161 attatgaaag tgctgcctcg caatttggca gtattgcttc agagcactaa tgtatgagcg
    14221 gacagttatc tttctgatcc atgctgcaca ggcgtgtatc ctggaatgag tccatgttta
    14281 tatgaagctg aaaggagatc gatcatgaag ttaagtggag tatgtataga gaccgataat
    14341 gcaccccgtc tcgcagaatt ttacaggatc gtcctgcagg aagagccttt tgttgaaggc
    14401 agccattact cattcagcaa atccctcaac ggcattgccc tcgttgtgag cattgaccgg
    14461 gcagcagtcc tggcacatca tacatccgat cagggaatta tgggcgttca cagcaaccca
    14521 ttccggaaac tctcccggga cctcattcat cgtcgtaagg caaacttcgg tgtttatgat
    14581 ccgatgatca tgttcgatcg cttttgtcgg acaagcagcc acacaggcat ggcatttttt
    14641 cgcatgcatc catacgccgg gctggaaacc agtcggcttc atcacagggc agatcagaga
    14701 tatacgaaat tattcggata tagctgccga attcctcatg gaagcaaatg ttgttgcgtc
    14761 catacaggca aagtccgcta tgaacagcca gcagtttttg cggaatcctg tcaaaaagtg
    14821 ccgccgtgta cccaaagggt ttcaaacagg catttatata ctccaatatc ccgttgtcct
    14881 taacaccctc gtccaggtat tggggcggta cgatgcaacg gatcgtttca ccatgataag
    14941 taaatccaac gatgatcttc gggctgggtg ttatcaccgt tatcagagac cgcggctgga
    15001 agccatattc atccgtgata gccataaaac ctgccatcca gtttataaac atgttatgcc
    15061 tgtccgttct taaagcctct atttcccgct tcagttcacc aaaacatgac acaggtacga
    15121 tggctcctct gtctccatgt gcagcgatct ctttcagcag tccttctttc ataccatacc
    15181 tccccgcagg accccagcga tcagactttt tccgcgccga agcctgctga tgcaatcctt
    15241 tgcgcaaatc actgcttttc actgacatta tatcagatat cttcattcag aacaaatgtt
    15301 cttttttatt tttaataaaa ttttcccatg ggccaaaaag aattgcatta aggatccatg
    15361 agaggagtca ggattctatc cccatttttt ccaggatctc ttcaggttcg cggatgatcc
    15421 tctccgcgcc gatcgatctc aggtaatccc tatcacggaa tccccaaccg accatgatga
    15481 catccacgcc cgcattcctg gccgtctggt aatccacctc cgagtccccg atgtatacca
    15541 cttcatttgg ggtagcctta aaatattcca tcacagaaat gacactgtcg ggatacggtt
    15601 tccttcgcat gccgtcttta tcacctactg aataatcaaa cagtccgccg aaatattctt
    15661 cacacagttt ctttacggca aggtccgcct tattcgaaac aaccgctgtc aggataccct
    15721 tatccttaag ttttttcagc acttccctga tcccgtcata tgggcgtgtt tttaccgcac
    15781 aatgatcctt atagtattca atgtatgttg tatatacttt atctatcatc ccttcatccg
    15841 cgccatccgg gacagcctgt tccacgagcc tgcggatgcc gtttccgaca aagcttttta
    15901 tctcgttcag cgacctttcc ggcatgccgt acttcttcag gcaatagttc attgaatcag
    15961 ccaggtcctc aagcgtatcc aatatcgttc cgtccatatc gaatatagca atcctgtatt
    16021 tcatgatcca ccccattctg agtttgctgc tccaatgtca tccgcataag tcaatgaaaa
    16081 gcctctgcct gatccgtttt tccaggccgg atttcatgcg gaatacgctg tgtcaaaaac
    16141 gtccataatt ctttatagca atcctgtctt tcattttcgc tgttgtaacc atatctgccc
    16201 acacaaaagt aaaaatgaac aaatcagcaa tggatcatgg ccacagttca cctgtcagtc
    16261 cttcaaggat cgatccgcag gcccaaggca tcagcgcaaa ccatgtactg gcaggcgtcc
    16321 cgtccggata acgctccgtc agcaaaccct cgctatggca aaaccgttcg gacatccatc
    16381 ccttcctgcc atagccgtac tcacggtcag caatcatgtg gcactggcag ctccagagaa
    16441 gcgtatcctc caggcggctc cggatgtatc catcgtcctg cctggacaag aagtaaacca
    16501 tctcatccat caccgaagag gacatggggt ggatgtgcgg gttgactacc gaggtaatgc
    16561 ttcctccgca gctggaccag ccagcagtgc tcaacggcgg tatttttatt ggcgagttgt
    16621 agcagaattt aaatgtgtat tcgtaataca gcgcatcccg caaatacatg agcaggtgct
    16681 cttccccggt aatctcgtga agatgtcgga ccgcccggat ataggcaaga acgccttccg
    16741 aatcgatatt cttgtcgatg tcaaggggac cgccgtaaca ctcctggtgc cggatatagg
    16801 cgtcatggta gtagtctgcg ctttgcagca gcgagggcag gtacgtcctg tcaccggtga
    16861 ggcaggtgta atatgccgcc gcagccatac accacgcacc ggaaaaagaa tcatattcca
    16921 gccccgctcc cgttttttca gatagtatat atggatactc tccgtccgca ttacggcttt
    16981 tttccgtccg cgcgatgacc ctttttgcaa aatccagcca gtcgccatga acgatattgc
    17041 gttcacgttt ttccaatgcg tatgccttca gtgccaggta cacgctctgc cccaccaggt
    17101 aagccgcatg ccccggcgtt cgctgccggt cgtaccacca tccccggttg ctccatttac
    17161 cgtcgtgttc tgccagatag ggcagatcgt tctgctcgtt tatggaataa cgaacgatat
    17221 ggtcgatgca gtccagggct tgctgccgaa tggtctcatc cttcaaacgc agcgatgagg
    17281 cgagcatcgg aactgccacg gccaggccgt tcgtccacgc gatggaaggc agcagcctgt
    17341 attcgaaatg gtctcccttg tcgaacacga agcatgaata agaatgtatc tccggcagcc
    17401 acgcatcacg ggcaatggca tttgccatgt cacgaacagt ctcctgcacg gtgcacaggc
    17461 ggcggggcga ctcatgatac ttccggtaaa cccatttcag tgcgtcatgg agcacgcgct
    17521 cgtcgcctcc aggaatatcg aagcagtata tccgaacgtc gaccgtttct ccctgctcta
    17581 tgcagaagca gtttttatcc agcggtgctc gcggataata ctgatgcgaa tcaaggaaaa
    17641 accacggcgc attctcgtaa cccagcgtat accaaacccc acccggctgt attgagcatc
    17701 cgaaacccgt gtactggtca aactctcccc ttataccggg ctcccattgt acccgccggc
    17761 tgccgcagcg catgtaataa ggcgacgccg caaaaccggt caggcgtccg tttccataag
    17821 caaaggcaca gggatgtgaa agccggtcac ttcgcaccat ccaccatggg gaggcgggaa
    17881 attcgccgtc ttcccggagg cgcgggcatt tgctgtccac aaccagcggt gtctccccac
    17941 ggttcgtgcc atacatgaag cctggcagga aaaaccgcgg cgcaggcgca tccacgggca
    18001 gtgaaatacg gatgacacct ttccacggtg tgctcccggt gttttttatc cggatgtata
    18061 cctgatacgg atcatccgat gaaccgccgc ctttctcaca gatcacagat acagtcaaat
    18121 gctgtgtttc cggcggaaaa tacgttatat cttccccaag aggacgtgca gtaataacag
    18181 gacttatcat ttgtttttct ccttttcgtt ctggtcatgc cgaaaatacc tgtttgacag
    18241 catcaaccca gtttactgtc attgattatt tttttcagat gatattctgc cgcttcagtg
    18301 aattcctggc tgtccagtat gctttcgatc tcatctattc tgcatatatg gactttatca
    18361 acttcaaact tataatcatc cgctcttgct gattctccat tgatttccct taagtcatga
    18421 tcaacataag cattaagtat agcaatgggc tgctttttaa caactccggc aacaataccg
    18481 gttatttcca ggtcagagca gtccaatctt aagttgagct cttcttccaa ctcccttaca
    18541 gcgcagcttc ttataggatc atccgtattc cagtcgataa gatccacggc tccggtggat
    18601 gtgacactat acaggttttt gcctattcct acatttccgg accgtttcac cagcattgca
    18661 tttccatatg tatctacaac ctggacactt atcgccaggg cattcgccaa gttgccattt
    18721 agtatcaggt cttcaaatcc attgcattct ttcaatgagg aatagtattc caataattcc
    18781 tgaagacttt taagatattt gtcatttttg cagtgttcct ctgcaaaaac cctgaagccg
    18841 gatatgtcca gcctgatgat attcgtcagc aaaaggctgt agaaatctgt gcgggatatt
    18901 acaagtacgc ttttatcacc ttctgtcctg acagcatcca gtcttacatt ttccgaattg
    18961 aaaaatttcc cgttcctgta agcgctccac atctcgacta tcaaatcttt agtttcgtct
    19021 gaaaatggga gctcatattt ttcatcgatt tttatatatt ggatcttctc ctttagcagg
    19081 tattcatata ctgccatatg cgcacctcac taaaatatac gtcaggtcat cttttttcct
    19141 cactcttgca acactattcc gagcatcatc ctgctggcat ttccgcagtg cgttaacgat
    19201 atgtttcagt ttttccgcat caaatatcga tccgtcaata gctgagatcc caaacaaatc
    19261 gaatgcctcc atcaatccgt cagaacaccc gaggcacatc gtttcaggat caatatctat
    19321 ctctccctgg atggcattgt cgatcccggc gcaatctacc gatccgatcc aatatccatt
    19381 ttcagtattc atatattttc ttgtatccct gagaagttcg atcttgtttt catatgtcag
    19441 ttttccgctc ttttttagcg cttcaatgcc ggatctgttc ttctctgtaa aaaccaggag
    19501 tctgctgtca gtcagcttta tgatctcgcc tttattcttt accaggagta cgcagtcgcc
    19561 caaaatataa tactcacatt tcgaaccggt catcctggcc agcagaactg caaaactggg
    19621 aagtttataa atctcatagt cttccgtttt caggctcagc agcttattcg tgtccgaaac
    19681 cgacttcctt aaaatatcgg ccagtctgtc tttcccgttg gcattatcat agatgcactg
    19741 gttcagttgc ctgacataac gcgcaacatc atcatcacat ccgaacaaat ttaaatggta
    19801 cagatcagtt gccccatcaa tgacccaaaa aatgttatcc ctgtatccat acgcgtcctc
    19861 gttatccggt ccgtctccgc gctctgaata actgctcaac acttccatcc aatcacccta
    19921 cttttttatc agctggccgg aatctataac tatattctgc cctgctattg atttattgca
    19981 tatcagcata tataccaact gcgctatgtc ctccggcacg gcaaattgcc ccgtaagggt
    20041 accctctctc aatttgctcc actctttttt tgattctctt aaggtaagtt ctgttacaac
    20101 tgttccgggc gataccacat tgacacggat acccttttct cccatttctt tggctatccc
    20161 atacatgaag cctgccagtc cggctttaga cgcgctgtat ccggccattc tgtatgccgc
    20221 catcccattt attgaagaga tcatagtgat cgacttgtct tccttcgaag cgatcaaata
    20281 cggcaataca gcatgcactg cgtttatatg tccaaccagg ttcagttcta tggatttcct
    20341 gatcacttcc atatccgtgt ctgaaaaatc gctccattct tcaggaagga caccacctgc
    20401 cacagtaact attccatcga ctgctgaccc gtcaaactcc ttcctgaaag cctttgtcaa
    20461 gtcgtcatat tttgtgacgt cgcaatggat aaatacctgg ttcgccttct tttcaggctc
    20521 ttttacatct accacgtata ctttgtaccc gttccttaga agtacttctg ctattgccgt
    20581 accaatagta ccgcttccgc cggtaacaat aatattcttc atcgctgtca tactccccaa
    20641 taacctcgtt cctggattaa aattatccta tcactgcagc tattactatt gctccaatta
    20701 tcagtattac tgcaaaaagc atgaacaaaa ccattgccct ggtatttgca ctggctcttg
    20761 cattttcccg ggcattatct ttttcacttg attgtatggt ttttcttgca ccgttgagaa
    20821 tgataaaatg aaaaaatgaa aacgccatta tcaaagtccc aagaaacagc agcataacta
    20881 cacaagctga gatcatatca agattcagaa cgacctgcga tgtaactacg atcagtgaaa
    20941 taatacctga aaagaaaccc agcaattcca gtgttccctt tttctgttcc tcaagttcct
    21001 ctttcaatgt ttttgaatcc tggaccagtg cttgtgtctg cttctggatc tcaacagtct
    21061 tttcctggtt atctttagcc tctttaagag caacatcgat ctccttcaat ctgcgttccg
    21121 tcttgcacct caataaagcc gtctcatagt caacgatttt gatagcgtaa tcttcgcggg
    21181 aactatcctc catatcaata gccctgttga aaagtttctc cgctttgtca tacaacctgc
    21241 ctgcttcttc aggacttaca taccggcatt ttgccatcag cagccttccc tcagtatagt
    21301 aatactttgc gaacttggga ttgaggctct ttgctttttc tatatattcc cccgcgagtt
    21361 cataattgtc ctcaaaagac tctttgtcac ccagctcgga agcataaaac accagctcac
    21421 agaagttatg gtagataccg gggtaattgg gatcatacct tttatccgtg ttgtatatat
    21481 cgatgcattt tttcgcagaa gcaagcgcct cgctcatatc cgccttattc ttattccttt
    21541 cttcctggta aaggatctgg ctgtttgtgt aataccataa ggggaaaatg ctgaagtcat
    21601 ccctgtaagt ttctacaata cgtttgtttt tcgatatatc tttttgatca tgatgatatg
    21661 tgaacaatat gtaaaatgct ttgaacctta acatgacgtc ttcattgcgg tcctctatga
    21721 aatctatgaa agctttgctg tcgagagccc ttttgttctt caggtacttg ttgattttcc
    21781 gggtaaaagc cgtggtctta tcatccggaa gatatcgcag cagccaatat atctcaagct
    21841 ccggatcgtt aactttcctg accgtgtaat gatatgcttt ctcaatatca gtttcattta
    21901 atttgcatac tgatgcggct atttcctgaa gctttccatc atcatttgat acttcaaagc
    21961 ccttatcttc gctgataaaa atctgcgtta taaagcgaac cagttttttt aatccatctt
    22021 ctgaatttgg ataagcaata tattcatcta tcagtttctg aacgtcatcc aaaactacca
    22081 ctgcattatc catttccatc gcctctcttc agctttgata taaggcactc cacccaaaca
    22141 ggcctcctga ggatccgcaa cgccgcgata cagctttttc ttacttttgc catatgcagg
    22201 cctcatgaca ttattataac aaagataaat gtaaaaaggg acataccttt ttcacaaagg
    22261 aatgtcccat tacaggggaa ggatatcaat attaaaactt atacagacct gcatgaccgg
    22321 ccgacgtagt acaaaacatg gcagcacccg gaaacgccgt ccgaatccct tctgttcata
    22381 gtccccggat gcttattaca gctcgacgct aaacatataa gcggtgctgt aattatcgta
    22441 cccattaaga aaaacttccg ctgtccagta tttatccgga taagggatgt tcacatcctg
    22501 gccaagtatt atgttacgag tgatgtcata catggcgccg cattcgtatc cttcgttttt
    22561 atgcatcgct tccatatcgc ccgaaaaaac agcgaatgcg aattgtgaaa tgtatggcag
    22621 tcctgcggca aaaccctttt cggctctttg gggtgtgaaa tcgaagtaaa cgaacccttc
    22681 aggcacggga gtatcggcct tcataaagcg tccccagaag ccatggcatg gttcggcatc
    22741 cactcctctg ccataatggt gtagtaacaa tacatcatag tcaaagcctg atttgtattc
    22801 gctcatggta tccaggacac cgaaaatttt cttaaggtcc tcgacagtat cccggccgtc
    22861 actttcacgg ctttctttcc cgatgaacct cagggcgggc atctttatat attcgaattt
    22921 ctcgaccttg aagccttcca gttgcttttc caccgcaccg ggcatgaaaa gtgtccattg
    22981 tactcttgca tcacaggttt tcagcaagtt tacataaccg aaaacatcaa cactgtctac
    23041 tccttcaggg cctgttttgc ctcccgcgat ttgctcgcgt atcttacggt cacgcatgac
    23101 cgagaaatcc gtaaacattt ttgataccac gtcccgaaga tggttttcat tagttttgcc
    23161 cgacagctcg tcatcaaacc tgtccaacgc attgaatacg gcttcattaa cgatccctgg
    23221 ctcgatatct ttcaggtatg cccggagctg tgcattctga ctcctgaatc gtgcaagtat
    23281 gccctgcatc aggatctgtt cattctccgc cagcttgtca tcggtccatt cggaatcgtc
    23341 aaggatccat tcggtcaggg catcgaaaac gttcggatta tgtactcttg ccttaaccgc
    23401 ccagtttacg atcctttgat atttttcagt accagacagc gattcgtctt tttgattttt
    23461 gaagtcggtc caataaaatt catcaatgaa tgagcgagac atttcaaagc ctccctgtac
    23521 ggttattttt attgtgaaag ggcggaacat cctgattttg ccaggctgca cctttgacgg
    23581 agggaaacca tgaaaccgtg tgaacgcctt ggaaaagctt tcctgggtat cgtactgata
    23641 acgcattgcg atatcgatta ttttacttcc tggtttcagc agatccagcg ctgctgaggt
    23701 caaacggcgg tttctgatat attcgcccac cgttacgcca aataccagat gaaaaagcag
    23761 ttggaaatgg gagcttgatg tataagcctg gttcgatatt tcgtcaatgc ttatgttatc
    23821 agtcaaatga tcttcaatgt atcgaatgct tttcgataag cattgtatcc agttcactcc
    23881 atccgcctcc tttcagcaag taatatatat cttttgaatc caaaagtcat gttttagaat
    23941 acgacgtttt gtcgggacac gatttccagc agttcttcag cttctttcct tgtcgggatc
    24001 ataatgcaat actcctccgt tacgcaaaat ctgtctcttc accagtccag ccatagctgc
    24061 gaaatgttgg attttcgtac atataaatca tatacttcct ggtaagttaa ttcaaaacaa
    24121 tatttgaaac atgcaggatt tttgatcgga tgcctgaaat gcgatatccg cataaaaatt
    24181 aaaaacaggt atctttgtcg agaatactat ataatacagc caatatcgaa aggagtggta
    24241 agtttgaagg tatcgtccac agaagttcag aataactttg gcaacctata taaccagcag
    24301 aaccgtccct gcggttacct aagcattccg ttcttttata tagaatttac gtctgcatcc
    24361 ttggtggtag agagactatt catactcaca gatgggatca agcgcaaata agggttcgtc
    24421 catcacaatt gctgaacttt taccaattgc cttactccac tgttccttat tccaaacttg
    24481 ctataattcc gtattccagc gattcttcgt atgtcctgcg aattaatgat tttgcacctt
    24541 caccgaatct tacctgtata gaacttataa aaccgtcata tattattttc cctttgaatg
    24601 gaagcaaaat agttttccat tctctttggt catatattct cattccaatc attaaaaatt
    24661 ctggatgaca tatagcaggt ccgttatctt cgttttgacc ggaaaatcac cgataactgt
    24721 gcaatttttt cacttatgtg cgcttttggc gcgattttat tgtaaatagt ccatacttaa
    24781 gttacataac acgtcgtatc ccttattatg tctgcctagt cgcgcccata aacgaaattt
    24841 tttcacacca acccgttatt tcccgggtta tttcccgcaa aaacctttac agcaggatga
    24901 aagacagagg aattccccta tatattaagg ggacatttct ctgctatcga agctcatctt
    24961 ccagcagaga agtgtctcct ttccttattc gccttacctg atcaaaaagg gctttacctc
    25021 ttcccgcaga ggctgtccct ttactgaggc gtgtcgggtt aagatgatca aaaggggaca
    25081 ttcctcttcc acagagaaat acccctataa attaagggga catttctcta ctatcaaagc
    25141 ccatcttcca gcagagaagt gtcccctttc cttattctcc ccttacctta acagcgactc
    25201 tcccgtcatt tccgccggct tctcgaagcc cagcagttcc agcatcgtcg gtgcaaggtc
    25261 tgcaaggcgt ccttc
//
LOCUS       DAOSCC010000007        24853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1450_length_24853_cov_5.198524, whole genome shotgun sequence.
ACCESSION   DAOSCC010000007 DAOSCC010000000
VERSION     DAOSCC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1450_length_24853_cov_5.198524"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PLG72_00975"
     CDS             <1..1140
                     /locus_tag="PLG72_00975"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ79408.1"
                     /translation="LFDDDGKTYYCGTGADKNGKQGIVLFEIDPDTGSILSDKQIISY
                     GSGGKFPEGPHIYKINGWYYLMIAEGGTEYGHMETMFRSRNIWGPYESCPNNPILTNR
                     NEMNTDIQCCGHADITEDENGNWWLVCLGIRKLPGGPLLHNLGRETFLAPVTWTSDGW
                     PQVGDMAVIRETMEGPLPKGETVRQSSDFSDDFTRPQLAPEWTFLRNPRMERYKTGDG
                     KLVIEGSRETLSDFEPSFIGVRQTEFDMTAQVTVSAKLESGNSKAGITVYYSKDNHYD
                     LYIERKEGRLWAVLSRRIMDLESVTGRVLLPDEPEMVTLLVETSAKEYLFRVIGSEGT
                     VTVGAGCTAGVCTEGTMTMTFTGTFIGLFATDTVAEFRSFSLTEK"
     gene            complement(1329..2156)
                     /locus_tag="PLG72_00980"
     CDS             complement(1329..2156)
                     /locus_tag="PLG72_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ79409.1"
                     /translation="MRFISWNIDSLNAALTGTSERSELSRKVLDTIREYDPDVIALQE
                     TKLPGDGPSKKHMEFLGEKFPDYAIVWNSSVAPARKSYAGTMFLYKNNLDPSVSFPKI
                     GAPGTMDYEGRIITLEFDRFFLTQVYTPNAGENLDRLADRQIWDKKFADYLVSLDKIK
                     PVVAAGDFNVAHRESDLAHPDSNRHSAGFTDEERQGFTNLLAKGFTDTFRHIHGDVTG
                     VYTWWSQIIRTSKINNSGWRIDYWLVSDRIADKVLKSEVIDSGPRQDHAPILLEVNL"
     gene            2463..3023
                     /locus_tag="PLG72_00985"
     CDS             2463..3023
                     /locus_tag="PLG72_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ79410.1"
                     /translation="MIVLIMAIENHDDREKAAEIYRLYSTTMFKIAKGILHDAHLAED
                     AVSEAFVRIIDNLEKINLSDRNKTKGFVVVIARHIAIDMLRRQKRYVPLDDHSDYTEC
                     KEPALSGLASDEACSGIADAIAKLNRSYSDILYFKIVFNYSNDEIAKILGISRDNVNV
                     RLHRARNALKQQLMKENDLYDRKGKK"
     gene            3001..3684
                     /locus_tag="PLG72_00990"
     CDS             3001..3684
                     /locus_tag="PLG72_00990"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HOJ79411.1"
                     /translation="MTEKEKNEIIFDAMLKIAVMEAFEQEMSELGSEGAEVTDRPSPE
                     LDRRVKRLIDKSYMKSKMQRYLKLTGKIAVCICIAFTLISLSVRATRNAIFNAIIEFQ
                     EKYARVNFGDSEAVNDIYRPTYLPEGFSEISTAKFGNTVMIKYSKADDAEIVLSQRPA
                     ESGALLVDNENTDCTEVEVSGATAYLFRGSSADDTSTLIWQANGTVFELTSRISSDEL
                     IKIGENLKK"
     gene            complement(3999..4202)
                     /locus_tag="PLG72_00995"
     CDS             complement(3999..4202)
                     /locus_tag="PLG72_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79412.1"
                     /translation="MVGPLTANHRKIEESVEKHADMLLRVAFMYMKNLSDAEDIVQGF
                     RLKLLFTQNQRDNCDHKAYESKS"
     gene            complement(4373..5197)
                     /locus_tag="PLG72_01000"
     CDS             complement(4373..5197)
                     /locus_tag="PLG72_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HOJ79413.1"
                     /translation="MNKKEETFYTSEYIGSLLSETSDSQNYGLPHELITLSNPIVETV
                     RSQAREIMRSLIGYKELMMMYKCAIKEVRTKLEILDTEFEIRHKRNPINFIVSRLKTT
                     SSIIEKLRKKNIPFSIRNIEEYVNDVAGVRVICSYVDDIYMIADALIQQDDIKLIRKK
                     DYIAQPKPNGYRSLHLIVSVPVFFAEQKKHMKVEVQIRTIAMDSWASLEHQLRYKQRV
                     AERPEIAEQLKECADILSEVDSRMLGIRMKIEENSSDPTEEEILLEKLSRIDRPIE"
     assembly_gap    5475..5574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5575..6344)
                     /locus_tag="PLG72_01005"
     CDS             complement(<5575..6344)
                     /locus_tag="PLG72_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOJ79414.1"
                     /translation="MEGSAEVPLEPGPISSEWYDWKWQFRNRITTVEQLEKVVLLSEK
                     EKRDISVCLNNFRMAITPYYASLMDPKDPKCPIRMQAIPTINEMKVLPYEMADPLDEE
                     RDSPVKNIVHRYPDRVLFLVTRMCSMYCRHCTRRRTVGEEDFAISDDEIDTAVDYIAK
                     NEAIRDVLISGGDPLTLDDGRLERIISKLRRIDHVEIIRIGTRVPVVMPMRITPGLLD
                     MLKKYQPIWINTHFNHPKEITPDSAKACTDIVDAGIPLG"
     gene            complement(6410..7474)
                     /locus_tag="PLG72_01010"
     CDS             complement(6410..7474)
                     /locus_tag="PLG72_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ79415.1"
                     /translation="MDDNESGGLSRLLHVGITFNLKKGKREERNRLSAGPEPAAPDAE
                     AEYDDISTVMAIKEALESRNCRVTLLEADEGLPLKLAEDKPDIVFNIAEGIQGRGREA
                     QVPALLDFYRIPYTGSDETTLCISLDKALTKRFVSTYGIRTPKYDVISGKQYRLAGRF
                     HFPVIVKPNAEGSSKGISDAAVAKNRKELQELVSKNINLYRQDMLVEEYISGREFTVG
                     IFGNGEDIHVFPPMEIRWINNGNQFNIYSYNVKQNYKEFVLYECPADIDKDTENEITC
                     TAKKIYEVLSCRDFARVDFRLSPEGKLYFLEINPLPGLAPGYSDFPMIAEFNGMDYNS
                     LIWNVLESALKRYGIKYSVG"
     gene            complement(7592..8860)
                     /locus_tag="PLG72_01015"
     CDS             complement(7592..8860)
                     /locus_tag="PLG72_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOJ79416.1"
                     /translation="MILPLVLFLLTYALLLAFSRYRAYIALASAALFVVFGILPVNKV
                     FFSVDWNVIMMIAGTMGVVSLFIESKMPSLLADMIIEKMPNVKWAIISLAFFASSISA
                     FVDNVATVLIVAPVALTISKKLNISPVPAIIAISISSNLQGAATLVGDTTSILLGGHA
                     DMDFLDFFVYKGRPGMFWVVQIGAIASTFVLLYVFRNEKQKIEAGDRTVVTDYFPSVL
                     LAGTILSLILASFIPEDRKPAIMNGLICVGFMAVGLAREAVRSRSLKSVKKVLKDIDY
                     FTLLLLGGLFIVIGGITEAGVVDAISGLFVKVSGSNVFLMYTLIVWASVLFSAFIDNI
                     PYVATMLPVTGGIANIMGIDPTVLYFGLLAGSTLGGNITPIGASANITGLGILHREGY
                     EVSAKEFMKISVPFTLAAVMSGYILVWLIW"
     gene            complement(9503..9628)
                     /locus_tag="PLG72_01020"
                     /pseudo
     CDS             complement(9503..9628)
                     /locus_tag="PLG72_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(9889..10263)
                     /locus_tag="PLG72_01025"
     CDS             complement(9889..10263)
                     /locus_tag="PLG72_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79417.1"
                     /translation="MLPGIGKMKKHIVIFCVPVMLVFAGIGAFVSVFPRNGAYIPAAE
                     GLMTVAGRTDYTPRVLHDPLAELGLRGKVLEPDEIQRILNENGYECEVRVTELYFDDL
                     AELKAYVDELKKKYSHLKIKEI"
     gene            10488..10790
                     /locus_tag="PLG72_01030"
     CDS             10488..10790
                     /locus_tag="PLG72_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HOJ79418.1"
                     /translation="MAIEVVPEWMANLEDEDLVFIKNFLLASGSLKEIAHQYRVTYPT
                     VRLRLDKLIQKIKISEKTENDPYIALIKRLAVNDKLDFNTAKILISEYKKVKGAEQ"
     gene            10895..11140
                     /locus_tag="PLG72_01035"
     CDS             10895..11140
                     /locus_tag="PLG72_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79419.1"
                     /translation="MIIPTVWFCIAVAGTGYRVYDRSLDAAYGNAATNVLGLIGEAVA
                     FFFIFNIPTAIFLLIYFTCRERKRRKKNLDKMNIQDL"
     gene            complement(11161..11571)
                     /locus_tag="PLG72_01040"
     CDS             complement(11161..11571)
                     /locus_tag="PLG72_01040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ79420.1"
                     /translation="MGKMIVKHSNPEGLFKSRAYSQVVTVSGSGKMIFIGGQNAVDAE
                     GRLVGLDDFELQTKQVLENIKIALASENATFNDVIKLNIYMADGCDPAVGFKAFRETV
                     GDLEKPPLITVLKVAGLANPAYMVEIDAIAAVEG"
     gene            complement(11716..13629)
                     /locus_tag="PLG72_01045"
     CDS             complement(11716..13629)
                     /locus_tag="PLG72_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79421.1"
                     /translation="MAAGPGGMGPGGPGAGRPGGAGRRRQGGPGGGPPGMRFAMSGGK
                     PKNASATINRLLSYIGKDKYKILFVFACVLGGSLTNLAGSYILRPIINNLVSADQTAA
                     EKVSNLITGILIMAGIYLVGVECVYLQQKIMIGVSQNALVKIREELFRRIQRLPLKYH
                     DTHTHGDIMSRFTNDLDAVGEMLNHTMTQIFSGIITLIGTVTLMFVTNWILALVIIIT
                     TPLLVYVGGMIGKQSRKYFSQQQQALGAVNGYIEETMAGQKVIKVFCHEETAIDEFEF
                     LSDELRKRQMKAQFFGGIMGPVMGNLSQVSYALSAAVGGILCLTANFDIGGLTIFTNY
                     SRQFSRPVNELSMQMNIIYAALAGAERVFEVMDEAPEAADPSDATEICSEVNKIAGAM
                     PIRGEVELKDVTFGYIPGVTVLKHINVTAKPGQKIAFVGSTGAGKTTITNLINRFYEI
                     EEGEILIDGINIKKIKKDSLRSNIAMVLQDTHLFSGTVRENIRYGRPDATDEEVIAAA
                     KVACAHSFIERLPEGYDTVLEGDGANLSQGERQLLNIARAAISKAPILILDEATSSVD
                     TRTEKLIERGMDQLMKNRTTFVIAHRLSTVRNADRIIVLEHGEIIEQGTHEELLAMGG
                     RYYQLYTGAIELD"
     gene            complement(13743..15479)
                     /locus_tag="PLG72_01050"
     CDS             complement(13743..15479)
                     /locus_tag="PLG72_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79422.1"
                     /translation="MRRYWKYVKPYLSAFIIGPILMIVEVIGEVVMPLLLSNIIDNGV
                     NGGRGIPYIIMMGAAMVITALIMMAGGVGGAYFAIKASTGFANDLRKDLFKQIQKFSF
                     TNIDQYNTGSLITRLTNDITQIQNMLQMMLRMALRAPGMLIGALIMAFALNPRLALTI
                     FCVLPLLSAAIYFILKIGFPRFSAMQKKLDALNTVTQENLTNIRVIKSFVRESFEEEK
                     FRNANIDLKNSALSAMNVVIFTMPVMTLAMNFTTLAVVWFGGNQVIAGSMTTGVLIAF
                     VNYVVQILFSLIMVSLIILNSSRTLASARRVNEVLRTDIDLTDDDASRKDLAVQRGKV
                     EFRGVHFKYYKNNEKWVLEDINLVINPGETVGIIGSTGSGKSTLVQMIPRLYDADRGE
                     VLVDDVNVKDYSLKNLRNGVGIVLQKNVLFSGTIMENLRWGDEDAAEEEVYRFAEYAQ
                     AHGFITSFEKGYETELGQGGVNVSGGQKQRLCIARTLLKKPKILILDDSTSAVDTATE
                     AKIRDKLKNELQDMTKIIIAQRISSVINADKIVVLDDGKIVGIGTHDELMQNCEAYSE
                     IYYSQMDKKVTA"
     gene            complement(15483..15962)
                     /locus_tag="PLG72_01055"
     CDS             complement(15483..15962)
                     /locus_tag="PLG72_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ79423.1"
                     /translation="MDGNQANEISDKKLIHQLINFAVKHRRIMQNYLDETGVYQAQHR
                     LLMEIARNPNFSQNDIARTMDVSAATVAVSLKKLEKGGYICRVTDEADNRLNKITITE
                     KGNAVVEQSRKIFRSADQKVFAGLAEEEKHTLSVLLHKLDANLDKMEEEIKLKKERT"
     gene            16323..16724
                     /locus_tag="PLG72_01060"
     CDS             16323..16724
                     /locus_tag="PLG72_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOJ79424.1"
                     /translation="MAVEWTHDLSVGVKHIDDQHKIWFQKANELFEAGKQQKAKEYIN
                     TMIDFLDEYTKQHFRDEEAYMEEIRYPGLDAQKKAHASFIDELAKLKKDYNEGGGNIL
                     VILNANRMVINWLTNHIRSMDKKIGEFVKNM"
     gene            complement(17074..17529)
                     /locus_tag="PLG72_01065"
     CDS             complement(17074..17529)
                     /locus_tag="PLG72_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ79425.1"
                     /translation="MHLGAIYLVVSDFEKTVGFYEKLLGIPVTHRNMDRFASFDFEGH
                     NISIMNGHFDADHPDKVVRKGEYTEEFDDLKKIALAPNTRKFVLNFWTEDLRKEHERI
                     RSLKLSDDLTGIKYIYNVSPYYYFQLTDPDGNVIEVTGAYSPEDGELED"
     gene            complement(17560..18717)
                     /locus_tag="PLG72_01070"
     CDS             complement(17560..18717)
                     /locus_tag="PLG72_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ79426.1"
                     /translation="MSRNGIHIIYGDDPGQMVKELLDTIKPEAEIAEDTLIGIKPNLV
                     VAKPSDSGATTSPEIVAGIIEYLESRGRSNIVIMEGSWVGDRTSEAFRVCGYEDLSRK
                     YDVPLIDLQKDKSRTYETDGMRISVCDRVMKVGYLINVPVMKGHCQTKMTCALKNMKG
                     CIPDPEKRRFHTMGLHKPIAYLNKIIKQDLIIVDGMNGDLNFEEGGNPVRMNRIFAGK
                     DPVLTDAYAAHLMGFDIKEIPYISIAERIGVGTADLENADIVELNREVPDRETGTTES
                     KRISGSRGMMPGIVQGSIPAGRVRQLAAYIAEDCACSACYGSLIHALERLREKGMLNR
                     LEEKLYIGQGFRDKRLNATGIGSCTSGFDKCVRGCPPKARDIVEYLEDQMQ"
     gene            complement(19133..19888)
                     /locus_tag="PLG72_01075"
     CDS             complement(19133..19888)
                     /locus_tag="PLG72_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOJ79427.1"
                     /translation="MINKLDLCTKALSLRKELGEDATSPVDIFSLALSIDKLTLVFYP
                     MGDHLSGICIKNDGYPVIAVNSSMSVGRQRFSMAHEFYHLYYDENARTTVCSIKIGTG
                     SDAEKAADQFASYFLMPPVALTEALRKIHGNSKGSLGIREIVMMEQFFGVSRQALLFR
                     LVDDGILTVQQTDQYRQGVISSAVSLGFDDSLYKPLPPEKRYRTYGYYIQTAEELMQK
                     GLISSGKYEQLLLTAFRPDLVYGNEVEGGELND"
     gene            complement(19878..20231)
                     /locus_tag="PLG72_01080"
     CDS             complement(19878..20231)
                     /locus_tag="PLG72_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ79428.1"
                     /translation="MDGLARRIGENVKYLREQSGLTQGCLAEYLNVDQSLISRIENNE
                     RSVTSDMLDKLSALFGVSIEQLTQETRPADRISFALRASEISDDDLETISSINRIALN
                     LNFMTRLLGGCGVDK"
     gene            complement(20740..22098)
                     /locus_tag="PLG72_01085"
     CDS             complement(20740..22098)
                     /locus_tag="PLG72_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ79429.1"
                     /translation="MMNKIKKAAANMLLDYIISDDPLAKLPKIFDIAEKLDRGNLHTV
                     QIDGVRKALLDRNSNWYTFVKKLYDEVDKKCIQKFVECFMINAHLTGFERTCEIEEKY
                     DCNVPWAILMDPTSACNLHCTGCWAAQYGNKNNLSYEMLDSICRQGKELGIYFYIFSG
                     GEPLVRKKDIIRLCSEHQDCYFFAFTNGTLVDDELCEEMLRVANFALAFSIEGDEEAT
                     DMRRGKGTYRKVIEAMERMRKHRLVFGYSTCYHRYNTDSVGSDEFVDDMIARGCRFSW
                     NFTYMPVGKDAHTDLIATPEQRAYMYKRIHEIRATKPIFAMDFWNDGEYADGCIAGGK
                     RYLHINAAGDVEPCAFIHYSNVNIHDVSLLDALRSPIFMAYRRRQPFNSNMLRPCPLL
                     DNPEMLKEMVKESGAKSTDMKAPEDVEVLCGKTTPAAKAWAEVADRLWAENPKSRKAV
                     SL"
     gene            complement(22259..22984)
                     /locus_tag="PLG72_01090"
     CDS             complement(22259..22984)
                     /locus_tag="PLG72_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOJ79430.1"
                     /translation="MMEKTTGSEARERIINAAIELFSRKGYDATTVNDIAGAANVNKA
                     LIYYYFKNKQDILDFMVDSILDDAVSGMLDYIQTNIVQMVRDGHLDIKPDRLHFANDE
                     AINAFLQNSYKFYERVIDYVIAKKEIIRIMVLESLKKGKHQNSLFRFMKFMSGNDENP
                     FFKTISSADQDFVYSEEMVLFKFFFSIIPIVSFAAYFDDYKAVSSLSDAELRRSFMRV
                     YHIINKSLISGSDILLRSHGPDA"
     gene            complement(23280..23999)
                     /locus_tag="PLG72_01095"
     CDS             complement(23280..23999)
                     /locus_tag="PLG72_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79431.1"
                     /translation="MGKVIRMPVFWIIICLAAAAGAVAVWFAASFGTPHDENAHRLKF
                     SEADRAETETLDLSSVNIIEEKTIDQDLSAYIFTDDEDGEYKGAVSTGGKSYYVGLVS
                     MEGTPGDLMGIKEVQVFGKKAVKFYGILGANYPQALYWLTGENPEDSVIQIDGNTTET
                     DLDNDTGNEIVATVGTIPETNIYVLKDSEISVSDINRSLGAKSVRLMDENNKLFEVYF
                     EPNKPEQFVYQDGSFVKKRSS"
     gene            complement(24060..24452)
                     /locus_tag="PLG72_01100"
     CDS             complement(24060..24452)
                     /locus_tag="PLG72_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ79432.1"
                     /translation="MQEVYEFLKKCGVYYLATVEGDQPRVRPFGTVDIFEGKLYIQTG
                     KVKNVSKQLQANPKAEICAWADGKWLRVTGKLIRDDRIEAKKHMLDNYPELKRLYSPE
                     DDNTEVLYFKDATAVFYSFTEDPKVIKL"
BASE COUNT         6277 a         6182 c         5342 g         6952 t
ORIGIN      
        1 cttttcgacg atgacggaaa aacatattac tgcgggaccg gagcagataa aaacggaaaa
       61 cagggcatag tgctttttga gatagacccg gatacaggct cgatcttaag cgacaaacag
      121 ataatatcat acgggtccgg cggcaagttc cccgaaggtc cgcatattta caagatcaac
      181 ggatggtatt acttaatgat cgcagagggc ggaaccgaat acggccacat ggaaactatg
      241 ttccgcagcc ggaatatttg ggggccatac gaatcatgcc cgaataaccc catcctgaca
      301 aaccgcaatg aaatgaacac ggatatccag tgctgcggac acgccgatat caccgaagat
      361 gaaaatggca actggtggct ggtctgcctg ggtatccgga aactgcccgg cggcccgttg
      421 ctgcacaacc tgggcaggga gacgttcctc gcgcctgtca catggacttc tgacggctgg
      481 ccgcaagtcg gcgacatggc cgtgatacgg gaaacaatgg aaggtcccct tcccaagggt
      541 gagaccgttc ggcagtcttc cgatttttcg gatgatttta cccggccgca gcttgccccg
      601 gaatggacat tcctgcgcaa tcccaggatg gagcgctaca aaaccggaga cggcaaattg
      661 gtcattgaag gaagccgcga aaccctcagc gatttcgaac cgtccttcat aggcgtacgc
      721 cagaccgaat ttgatatgac cgcgcaggtg accgtttcgg caaaactgga gtcaggaaat
      781 tcaaaagccg gcattacggt ctattactcc aaggataacc actatgatct ctatatcgaa
      841 cggaaagaag gaagactgtg ggctgtcctt tcaagaagga tcatggatct cgaatctgtc
      901 acgggcaggg tcctgctccc ggatgaacct gaaatggtga ccctgctggt ggagacaagc
      961 gcgaaagaat atctgttccg cgttatcgga agtgaaggta cagtcaccgt cggagcggga
     1021 tgcacagcag gagtctgcac cgaaggtact atgacgatga catttaccgg cacattcatc
     1081 gggctgtttg cgacagatac agttgcagag ttccgctcat tcagcctgac ggaaaaatga
     1141 ttgccgggcc agccggccaa tcaatggttt ccggggagcc tggcaaggga actggtatga
     1201 aatgaccgtc taagtgtccg acggccctga aagtgtccga aacgatattg gagccggtca
     1261 tatcgcagtc gatcggtcat gcctgtaacg tttcggacac ttttactttt cctggccaca
     1321 ttgcatatct acaggtttac ctcgagcagt atcggggcgt gatcctgcct gggccccgaa
     1381 tcgatcactt cagatttgag aactttatcg gcgatccggt cgctgaccag ccagtaatcg
     1441 atcctccagc cggaattatt tatcttgctt gtccttatga tttgggacca ccaggtgtag
     1501 acaccggtca catcgccatg gatatgcctg aacgtatccg taaacccttt tgcaagcaga
     1561 ttggtgaagc cctggcgttc ttcgtcggta aagccggccg aatggcggtt gctgtcaggg
     1621 tgggccaggt ccgattccct gtgcgccacg ttgaaatccc ctgccgcgac aaccggtttt
     1681 attttgtcca gcgacacaag gtaatcggcg aatttcttat cccagatctg gcggtccgcc
     1741 agccggtcca ggttctcgcc cgcatttggc gtatagacct gcgtcaggaa gaaacggtcg
     1801 aattccagcg tgattatacg accttcatag tccatagtgc ccggcgcacc gattttcgga
     1861 aatgaaacgg atggatccag gttatttttg tacagaaaca tagtgcctgc atagctcttc
     1921 ctggccggcg cgacagagct gttccacacg atcgcgtaat ccgggaactt ctcccccagg
     1981 aactccatgt gcttttttga cggcccgtcg ccgggcagct tagtttcctg taaagcaatc
     2041 acatcaggat cgtattccct gatcgtatcc aatactttcc gcgacagctc actgcgctcc
     2101 gaagtgcctg tcaatgccgc attcagcgaa tcgatgttcc acgagatgaa tctcacagcg
     2161 ttatcctccc ccgtatggct ctctggggta tatgataaca taaaaagtgg tgcctggcac
     2221 cactttcagg acaccctgtt cgactgtcgt taacagtttt cgtctgcaag gcccttcagg
     2281 gagacattat cactgaattt gaaaagcggc aggcatcatc agcatgaaga gtactttgga
     2341 ccctgtctca taaaaataat aagcagctat ggaaatgcat tacaaaaatc catgattata
     2401 tagtataatt taactgcatt ttttgtatgt tttgtttgaa ttcgcagccg gagttgattg
     2461 ttttgatcgt actgattatg gcaatcgaaa accatgatga cagggaaaaa gctgccgaga
     2521 tttacagact ctacagtaca acgatgttca aaattgcaaa aggcatcctg catgatgcac
     2581 acctggcaga ggatgccgtg tcagaggctt ttgtccggat aattgacaat cttgaaaaaa
     2641 taaacctgtc tgaccgtaat aaaacaaagg gattcgtcgt tgtaatagcg aggcatatag
     2701 ctattgacat gctgagaagg caaaaacgat acgtaccgct cgacgatcat tcggattaca
     2761 cggaatgcaa ggaaccggca ctcagcggct tagcctctga tgaagcatgt tccggaatag
     2821 cagatgcgat agcaaagtta aacaggagtt attccgacat cctgtacttt aaaatcgtat
     2881 tcaattactc aaatgatgaa atcgccaaaa tactcggtat cagccgggat aatgtgaatg
     2941 tccgtctcca cagggcaaga aacgccttaa aacagcagct tatgaaggag aatgacctgt
     3001 atgaccgaaa aggaaaaaaa tgagataata ttcgatgcaa tgctgaaaat agcggtcatg
     3061 gaagcatttg aacaggaaat gtccgaattg ggatccgaag gagcggaagt gacggacaga
     3121 ccttcaccgg aactggacag gcgcgtaaaa agactgatag acaaaagtta catgaagtca
     3181 aaaatgcagc gatatttgaa gcttacaggc aagatcgccg tatgcatatg tattgctttc
     3241 actttaatat cgttaagtgt cagggcgacg agaaatgcta tattcaatgc aatcatagaa
     3301 ttccaggaga aatatgccag ggtaaatttc ggagattcgg aagcggtaaa cgatatatac
     3361 cgtccgacat atctgccgga agggttcagc gaaatttcga cggctaaatt cggaaatact
     3421 gtcatgatca aatattcaaa agcggatgat gccgaaatag tccttagcca gcgccctgcc
     3481 gaatcaggag ccctattggt ggacaatgaa aacacggact gcacggaagt ggaagtttcc
     3541 ggcgctacgg cctatctgtt cagaggatcg tcggccgacg atacaagcac ccttatttgg
     3601 caggcaaatg gaacagtatt tgaactgact tccaggatca gcagcgacga attgatcaag
     3661 atcggtgaaa acctgaaaaa atgatatgaa atatttccct taggagtgta attttaggga
     3721 tcctttttcg tttatatagt gaaggtaaat gacacctcaa atataagcat tttactgttg
     3781 atcgttaaaa atcaggagca attgaaagga gtgtggacat tttgaagtac aacaacactt
     3841 atccgataat agcagccatc tcgcttattc tgtattctat cttgagtttt attgaggaag
     3901 ttgctgaaaa aagcattctc gcaaggctgc acataagcgg ggcaagtttt aattacgaca
     3961 cgttccggct aaacacatcc ggagcaaaaa acatattatt agctcttgct ttcgtatgcc
     4021 ttgtgatcac aattatctcg ttgattctgc gtaaaaagta attttaaccg gaagccctgg
     4081 actatgtctt cggcgtcgct caggtttttc atgtacatga aagcgaccct gaggagcata
     4141 tctgcgtgtt tttcaacaga ctcttctatt tttctgtgat ttgcggtcag tgggcccacc
     4201 atttttcctc ctcacatata atacaaatca agtgggcaaa aaaacgcatt gaatataaat
     4261 attttatcat tctttcagca aatggatatt acagatcgat tttacaagat gtcctcacaa
     4321 aaaactgggt gccttgttca ggcacctcat ttgactgcag tgatcatacc agctattcaa
     4381 tcggcctgtc gatcctgctc agcttttcaa gcaggatctc ttcttccgtc ggatcgctgc
     4441 tgttttcttc tatcttcatc cgtatgccga gcatccggga gtcgacctcc gacaaaatat
     4501 ctgcgcattc cttcaactgt tcggcgattt ccggcctttc cgctactcgc tgtttatatc
     4561 tcagctgatg ctcaaggctc gcccaggagt ccattgcaat cgtcctgatt tggacttcta
     4621 ctttcatatg tttcttctgc tcggcgaaaa agaccggaac actgactatc agatgcaggc
     4681 tgcggtaacc gtttggtttt ggttgggcaa tgtaatcctt tttacgaata agctttatgt
     4741 catcctgctg tatcagcgca tcagcaatca tgtagatatc atccacatag gaacagatta
     4801 cccggactcc ggctacatcg ttgacatatt cctcgatatt cctgatcgaa aacggtatgt
     4861 tctttttcct cagcttttca attatgctcg aagtggtctt aaggcgcgaa acgataaaat
     4921 tgatgggatt tcttttatgc ctgatctcaa attctgtatc caggatctcc agtttcgtcc
     4981 tgacttcctt tattgcgcat ttgtacatca tcatcagttc tttgtacccg atcagggacc
     5041 tcataatctc tctggcctgg ctgcgcactg tttctactat tggattgctg agagttatca
     5101 attcatgcgg aagaccatag ttttgcgagt cggaggtttc tgagagcaaa gatccgatat
     5161 actccgatgt ataaaaagtc tcttcttttt tattcatttc ccatctcctt gcctttttta
     5221 gatccaggtt ctgtgaatat gcgctgcctt ttacacagac tattatattg acgcaacatg
     5281 aactgaatat aaaccgcata atgaaatata aaagcggcct ctgcggccgc ttgctgcatt
     5341 aaaatatgtc aattttcata gttcatcatc tttactatgc attgcaatcg catataagat
     5401 cagccattca tatctcaatc gctttaaatc ctgatatgcg gataagtgta tattctccct
     5461 tcatagttcc tcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccagc
     5581 gggataccgg catccacgat atctgtgcag gctttggctg aatccggggt gatctctttt
     5641 ggatggttga aatgggtatt tatccagata ggctgatatt ttttcaacat gtccaagagc
     5701 ccgggtgtga tgcgcatggg cataacgacc ggcactctcg tcccgatacg gattatctcc
     5761 acatggtcta tccttcgaag ttttgagatt atcctctcaa gcctgccgtc atccagcgta
     5821 agcggatccc cgccggatat cagcacatcg cgtattgctt cgttctttgc tatataatcc
     5881 accgctgtat cgatctcatc atcgctgatc gcaaaatctt cctcacccac agtccgtctc
     5941 ctggtacagt gtctgcagta cattgagcac atgcgggtta ccaggaacag tacccggtcc
     6001 gggtaacggt gcacgatgtt tttcacaggg ctgtcccgtt cttcatccag cggatcagcc
     6061 atttcatatg gaagaacctt catctcgttt atagtcggga tcgcctgcat ccggatcgga
     6121 catttcggat ccttcggatc catcagggat gcataataag gagtgattgc cattcggaag
     6181 ttgttcaggc aaacggatat atcacgcttc tctttctcgc ttaacaggac cactttttcc
     6241 agttgttcca cggttgtgat cctgtttctg aactgccatt tccaatcata ccattcactg
     6301 ctgataggcc caggttctag cggtacttct gcactgcctt ccatattcat tcccccttga
     6361 atgaagtttt ataatattta agtaaaactt tccggttgca aaataaatct cagccaacgc
     6421 tgtacttaat gccgtacctt ttaagagcgc tttccagcac attccatatc agcgagttat
     6481 agtccatgcc attgaattcg gctatcatag gaaagtcgct gtaacccggg gccagtccag
     6541 gcaagggatt gatctccagg aaatacagct ttccttccgg ggaaagcctg aaatcgaccc
     6601 ttgcaaagtc cctgcaggat aaaacttcat agattttttt cgccgtgcat gttatttcgt
     6661 tttccgtatc tttgtcgata tccgccggac attcatacag gacgaactct ttatagtttt
     6721 gcttgacgtt atagctgtaa atgttgaatt gattcccatt gtttatccag cgtatctcca
     6781 tcgggggaaa tacgtggata tcctcgccgt ttccgaagat accgacggta aactcacggc
     6841 cgctgatgta ttcctcgacc agcatgtcct gcctgtacag gtttatgttc ttcgacacca
     6901 gttcctgcag ttctttccgg ttctttgcga ccgcagcgtc ggatatgcct ttgctggaac
     6961 cttccgcatt tggttttacg atcactggga aatggaatct gccggcaagt cgatactgtt
     7021 ttccggagat gacatcatat ttaggcgtcc tgatgccata cgtggagaca aaccgctttg
     7081 tcagcgcttt atccagcgaa atgcacaaag tcgtttcatc cgaacctgta taggggatcc
     7141 tgtagaaatc aagcaaggcg ggtacttgag cttcgcggcc tcttccctgg attccttccg
     7201 ctatgttgaa tacgatgtcg ggcttatctt ccgccagctt caaaggcagt ccttcgtccg
     7261 cttccagtaa ggtcacccgg cagtttctgc tctcaagggc ctccttgatc gccatgactg
     7321 ttgaaatatc gtcatactct gcttcagcgt ccggagcagc gggctccggg ccggcggaca
     7381 ggcggtttcg ttcctcccgt ttgcctttct taaggttaaa ggttatccca acatgcagca
     7441 accgggagag tccacctgat tcattatcgt ccatttttac gcctcgattc tgcccacttg
     7501 tatcgtttcg agtttagtcc tatttacgag caatgtcaat actaaatatg ataaatttca
     7561 tatgcgcatt atacagtttg tatcggcatg tctaccaaat gagccaaacc agtatatacc
     7621 cggacataac agccgccagc gtaaaaggca cgctgatttt cataaattcc ttcgcgctga
     7681 cctcataccc ttccctgtgc agaatgccaa ggcctgtgat gtttgcggac gctccgatgg
     7741 gcgtgatgtt gcctcccagc gttgatccgg ccaggaggcc gaaataaagg actgtagggt
     7801 ctatccccat aatgtttgcg ataccgccgg taacaggaag cattgtcgca acataaggga
     7861 tgttgtctat gaacgccgag aataacacag aagcccagac aataagcgta tacatcaaaa
     7921 atacattgct tccgcttact ttgacaaaaa ggccgctgat cgcgtcaaca acgcctgcct
     7981 ccgtaatgcc gcctattacg ataaaaagac cgcccagcaa aagaagagtg aaatagtcta
     8041 tatcctttaa tacctttttc accgatttca ggctgcgtga cctgacagcc tcacgggcca
     8101 gcccgacagc cataaagccg acacaaatca agccattcat aatcgcaggc ttcctgtctt
     8161 ccgggatgaa agaagcaagg atcagcgaca gtatggtacc ggcgagaagc acggacggga
     8221 aataatcagt aacgaccgtt ctgtccccgg cctctatttt ttgcttctca ttgcggaaca
     8281 cgtataaaag gacgaacgtc gaagcaattg cgccgatctg gacgacccag aacatgcctg
     8341 gcctgccttt gtatacaaag aaatcaagaa agtccatatc agcatgtccg ccaagcagga
     8401 tggaagtagt gtcgccgaca agcgtagcag cgccctgcag gttagatgat atggatatgg
     8461 caataatggc cggcactggg gagatgttca gctttttcga tatggtaagc gcaacaggag
     8521 caactatcag aaccgttgcg acattgtcca caaatgcgga aatggaactt gcgaagaaag
     8581 ccaatgaaat tatcgcccat ttgacgttcg gcatcttctc gatgatcata tcagcaagaa
     8641 gcgatggcat tttcgattcg ataaacagcg agacgacgcc catcgtgccg gcgatcatca
     8701 ttattacgtt ccagtctact gaaaagaata ccttgttcac gggtaaaatg ccaaatacga
     8761 caaacagtgc tgcagaagcc aatgcgatat atgctctgta cctggagaat gcaagcagca
     8821 gtgcatatgt cagcagaaac aatacaagcg gtaaaatcat ctgaaacacg ctcccggttt
     8881 ttgagagtga ccgtcatcaa aaaatgatta taccattgct gtcgcttatt gcaagtaatt
     8941 gtcaaaatca ttcgaaatgg accccatttg atattcgtga tatacattgc tgtctggaag
     9001 tcaacgtaat tttttaatgc cggacatggc aatagcagtt gtgtgccgaa aaaaatcctt
     9061 ccggtatatt tctcgcattg ataccggaag gatttaagga ttcaataagt aaccaggaaa
     9121 tatgtatcaa tgtgccgtgc tcggtaccat gcgctctccg gcacatcatc gaagatcacc
     9181 tgcttccagc tttttccgta atagctgaaa tgtgtattgg caaaaatgac cgggcctgtt
     9241 tcggtgatgt accatccgtt gggtatggtt tttacattgc cagaaccgtc aaagtacctg
     9301 actatgatgc attctgagtt ctccagttca tctgcagtcg gggtatatgg tatccttacc
     9361 gttgcacggg ctttgcggtt gctccagttg acaggtttgc atcgatagcc agcgtgagct
     9421 gaacgaccgg cctgccgcct tgggggatag agcaaatatg attaaaataa gtaagaatat
     9481 tatcattttg cgttactgta tatatttcaa accagaatgt actgcccctg ccctcttcgg
     9541 attcgacccc gtacctgccg ccgtgcatgt cgattacctt tttgacgata gaaagcccga
     9601 gtcctgtgcc cattattggt cttttatgct acccttcggg agcaaacatc tatatggtcg
     9661 ggtaaattct gaaaagtttt gcatgaggaa ctacattcac gcaattgaag aagccgggtg
     9721 tacaaaattc gaattgttgt gtgccaaaat ggcatatata ttgttaattg acaataataa
     9781 agctgacata atgtggtttt tcctagtatt atgtttacat ataatcgata agtaacctaa
     9841 cataatttat tatgtctacc caacaattca gattttgtac acccagcttc atatttcctt
     9901 tatcttcaaa tgcgagtatt tctttttcag ttcatcgaca taagccttaa gttccgcgag
     9961 atcatcaaaa taaagctcgg taactctgac ctcgcattcg taaccgtttt cattcaggat
    10021 tctctgtatt tcatctggtt caagcacttt ccccctcaat ccaagttcgg ccaggggatc
    10081 atgcagaact ctcggcgtat agtccgtcct gcctgcaact gtcattaaac cttcggcagc
    10141 cggaatataa gcgccattgc ggggaaagac cgatacaaat gctccgatgc cggcaaaaac
    10201 aagcattacg ggaacacaga aaatcacgat atgctttttc atttttccga tacccggcaa
    10261 catatccatc ctcccatccg atatgcctga tttgaaggta aaagccatgt ccgtaacaga
    10321 ataatccctg gttttattat gctgcataaa tatggaatat gtcaataaca tatgaaagcc
    10381 cgcaaggtat tattcattaa aattaataat ataaataaaa ctgtgctttt ctcttgaatt
    10441 taataatatt aaagtatata cttaatattg ttaaaggagg tgctgtcatg gcaatagaag
    10501 tcgtccctga atggatggca aacctggaag acgaagacct ggtattcatc aagaatttcc
    10561 tccttgcttc cggatctctt aaagagatag cacaccagta cagggtgaca tatcctactg
    10621 tacgtctccg gcttgacaaa ttgatccaga aaataaaaat cagcgagaaa acggaaaacg
    10681 atccatacat cgcactgatc aaaaggctgg cggtaaatga caagctggat ttcaacacag
    10741 ccaagatact gatcagcgag tataaaaaag taaagggggc agagcagtaa tggcaatgaa
    10801 aatcgttcta acaattcctg cattatttat tatctttatg ctttttatat tgctgcaaat
    10861 cttcctgtcc aggatggaaa acaggtggcc cgggttgata atccctacag tctggttctg
    10921 tatagctgtc gccggtacag gttatcgagt ctatgaccgg tcactggatg ccgcatatgg
    10981 aaatgcagcc acaaatgtcc tcggattgat cggtgaagcg gtcgcttttt tctttatttt
    11041 caacattcct acagctatct tcctcctgat atatttcacc tgcagggaaa gaaaaaggcg
    11101 caagaagaac cttgacaaaa tgaacataca ggacctgtaa tgagcgtcgg cagactttct
    11161 ttatccttcc actgctgcaa tggcatcgat ctcgaccatg tatgcaggat ttgcaaggcc
    11221 cgctactttg aggaccgtta tcaggggagg cttttcaaga tcgccgaccg tttccctgaa
    11281 tgccttgaac ccgacagccg ggtcgcaccc gtcggccatg tagatattga gcttgatcac
    11341 atcgttgaag gtggcgtttt cagaagccag ggcgatcttg atgttctcca ggacctgctt
    11401 tgtctggagt tcaaaatcat cgagccctac cagccggcct tctgcgtcga ctgcattctg
    11461 gccgccgata aagatcattt tcccgctgcc gctgaccgtg accacctggc tgtaagccct
    11521 ggatttgaat aaaccttcag gattgctgtg tttgacgatc attttgccca tcacatcttt
    11581 cctccttcaa acaacaggta tgatattgcc gcatgtactt atagattact tttatgagtt
    11641 taccacttac acccgatata taatcatgtt tataaccgcg gcaataccac ctgttctgcc
    11701 gaaggatttc actttctaat ccagttcgat cgccccggta taaagctgat agtaccttcc
    11761 gcccatcgca aggagttcct cgtgggtgcc ctgctcgatg atctcgccat gctccagcac
    11821 gatgatccgg tctgcattac ggacggtaga cagcctgtgc gcgatcacaa atgtcgtcct
    11881 gttcttcata agctgatcca taccgcgttc aattagtttc tcagtccttg tatcaacaga
    11941 gcttgtagct tcgtcaagta taaggatggg agctttcgat atggcagccc gcgcgatatt
    12001 aaggagctgt cgctctccct ggctcaggtt ggcgccgtcg ccttcgagca ccgtgtcata
    12061 gccctccgga agcctctcga taaaggaatg ggcgcacgcg actttggcag cagcaatgac
    12121 ctcctcgtca gttgcatccg ggcgtccgta ccggatgttc tccctgaccg tccctgagaa
    12181 caggtgcgta tcctggagca ccatcgctat attgctcctg agcgaatcct tttttatctt
    12241 ttttatgttg atgccgtcga tcagtatctc tccctcttcg atttcataga acctgttgat
    12301 aaggttcgta atggtcgtct tccccgcgcc cgtggatccg acgaaagcaa ttttctggcc
    12361 tggttttgct gttacgttta tatgcttaag tacggttacc ccggggatat agccgaaagt
    12421 cacatccttc agttccactt cgcccctgat tggcattgca ccggcaatct tattcacttc
    12481 agagcatatc tctgtcgcat cggacgggtc cgctgcttca ggcgcctcat ccattacttc
    12541 aaaaactctt tccgctcccg ccagtgcagc gtaaattata ttcatctgca tggacagctc
    12601 attaaccggc cgtgagaact gccttgaata attcgtgaat atggtcagcc cgccaatatc
    12661 gaagttcgct gtcaggcaca ggatacctcc gactgccgcc gacaacgcat agctcacctg
    12721 gctgaggttg cccatgacgg gtcccattat gccgccgaaa aactgggcct tcatttgcct
    12781 cttgcgcaat tcatcgctca ggaattcgaa ttcatctatt gccgtttcct cgtggcagaa
    12841 tacctttata actttctggc ctgccatggt ttcttcgata taaccgttga ccgcgccaag
    12901 cgcctgctgc tgttgcgaaa agtatttcct gctctgcttt ccgatcatgc cacccacgta
    12961 gaccagaagc ggggttgtaa tgataatgac caatgcgagt atccagttcg ttacaaacat
    13021 aagcgtcaca gtgccgatca gtgtgattat tcccgaaaag atctgcgtca ttgtgtgatt
    13081 gagcatctct ccgaccgcat caaggtcatt tgtgaatcgg ctcattatgt cgccgtgcgt
    13141 atgcgtatca tgatatttca ggggaagcct ctggatcctg cggaacagtt cttccctgat
    13201 tttgaccagt gcgttctggg acactcctat catgatcttt tgctgaaggt aaacacattc
    13261 gacaccgaca agatatatcc ctgccatgat caggataccg gtaataagat tgcttacttt
    13321 ttctgctgcg gtctggtcgg ccgacaccag gttgtttata atcgggcgca aaatatagct
    13381 tccggccagg ttggtcaggc tgcctccgag cacgcatgca aatacgaaca ggattttata
    13441 tttatccttt cctatatagg atagcagcct gtttatagtc gctgaagcgt ttttgggttt
    13501 tccgccggac atggcaaagc gcatacccgg aggtcctccg ccaggaccgc cctgcctcct
    13561 tcttccggcg cctcccggcc tccccgctcc gggaccgccg ggccccattc cgcccggacc
    13621 ggcagccata tttatattcc catacctgcg cagcagtatc tctttccttt cttcgctgat
    13681 ccgtcccttt tcgctggtac ggcaaatttc accgacacgc tcctcttctc tgatcctgtc
    13741 cattatgccg tcaccttctt gtccatttgt gagtagtaga tttccgaata tgcctcgcaa
    13801 ttttgcatca attcgtcgtg ggtacctatg ccgacgattt taccgtcatc gaggactacg
    13861 attttatctg cgttgattac cgacgaaatc ctctgtgcga ttattatttt cgtcatgtcc
    13921 tgcagttcat ttttcagttt atccctgatc ttcgcctcgg tcgcggtgtc aactgcgctg
    13981 gtactgtcgt cgaggatcag gatcttcggc ttcttcagca gggtccttgc aatacacagc
    14041 ctttgtttct gtccgccgga gacattgacg cctccctgcc caagctcggt ctcataacct
    14101 ttttcgaaag aagtgatgaa tccgtgtgcc tgtgcatatt ccgcaaacct gtacacctct
    14161 tcttcagcag catcttcatc gccccatctg aggttttcca tgatagtacc tgaaaacagc
    14221 acgttcttct ggagcacgat gcctaccccg ttgcggaggt ttttcagcga atagtctttg
    14281 acattgacgt catccaccag gacttctccc cggtctgcgt catacagcct ggggatcatc
    14341 tggacgagtg tcgacttgcc gctgccggtc gaacctatga taccgaccgt ctcgcccggg
    14401 tttatcacaa ggttgatatc ctcaaggacc catttttcat tgtttttgta atatttgaaa
    14461 tgaacgcccc tgaactcgac cttgccgcgc tgaacggcca gatctttccg ggaagcgtca
    14521 tcatccgtca gatcgatgtc agtgcgaagg acttcattga cacgtcttgc agatgcaagg
    14581 gtcctggaac tgttgagtat gatcagggaa accattataa gtgaaaagag gatctggacc
    14641 acatagttca caaacgcaat cagaacgccg gtggtcatgc ttccggcaat gacctggttc
    14701 ccgccgaacc acaccactgc cagggtcgta aaattcatag cgagcgtcat gacaggcatg
    14761 gtgaatatga ctacgttcat tgcgctcaat gcgctgtttt tcagatctat attggcattt
    14821 ctgaatttct cctcctcaaa gctttccctg acaaatgatt ttatgaccct tatattagtc
    14881 aggttttcct gtgtgacagt attgagggcg tccagctttt tctgcatggc tgaaaaacgc
    14941 ggaaaaccga ttttcagtat aaagtagatc gcagccgaca gcagaggcaa aacacagaat
    15001 attgttaatg ccagcctcgg gttcagggca aaagccatta tgagcgcgcc tatcagcatg
    15061 cccggagctc tcagggccat tctcagcatc atctggagca tgttctgtat ctgtgtgata
    15121 tcatttgtaa gccgtgtgat cagagaacca gtattatact gatcgatatt ggtgaaggag
    15181 aatttctgga tctgtttaaa caggtctttc ctcaaatcgt tcgcgaatcc tgtggacgcc
    15241 ttgatggcaa aataggcgcc tcccacgcct ccggccatca tgatgagcgc cgttatcacc
    15301 atcgctgcgc ccatcattat aatataagga atgcccctgc ccccatttac gccattatct
    15361 atgatattgc taagcagcag aggcatgacc acctcgccga taacttcaac gatcatcagg
    15421 atcggtccta tgataaatgc agataaatat ggtttgacat atttccagta ccgtctcata
    15481 cattacgtcc tttctttttt cagctttatt tcttcttcca ttttgtccag gttcgcatcc
    15541 agcttgtgca gcaaaacaga cagggtatgc ttctcttctt ccgcgaggcc cgcaaaaacc
    15601 ttttggtctg ccgacctgaa aatctttctg ctttgttcta caacagcgtt ccctttttca
    15661 gtaatggtga tcttgttgag ccggttatcg gcttcatccg ttaccctgca gatgtaccct
    15721 cccttttcca gcttctttag cgatactgcg actgtcgccg cagatacatc cattgtcctg
    15781 gcaatatcat tctgtgaaaa attcggattg cgtgcgattt ccatcagcag gcgatgctgt
    15841 gcctggtaga cgccggtttc atccaggtag ttctgcatga tcctcctgtg cttcacggca
    15901 aagttgatca actgatggat caatttcttg tcgctgattt catttgcttg attgccgtcc
    15961 atctttccgg attgctcacc gcctttcgcc tggtaatgca gttataaaat cattcctggc
    16021 atgtcaatcg ttaccatgct aattattaac tagctaacga ttgaatatta tagcatttat
    16081 tttctgccga tgtcaatccc gataatacat ccgctattca gccgttcaac cgttctgcat
    16141 aacagcgccc cggcatgact gctgtaagcc ggatcaggga aaaggcactc ctgtcagtac
    16201 agccagagca gaagcttatg acctgtttcg acacaatacg ccattttccg aaaatatatt
    16261 gaactcgtcc cggataacct gtaaaattta agtaacagca atgatccgga agggggatat
    16321 atatggctgt agaatggact catgatctgt cggtaggagt aaagcatatc gacgaccagc
    16381 acaaaatctg gttccagaag gcgaatgaac tgtttgaagc aggaaagcag cagaaggcaa
    16441 aagagtacat aaatacgatg atcgatttcc tggacgagta tacgaagcag catttcaggg
    16501 atgaagaagc ctacatggaa gaaatccgct accccggact tgatgcccag aagaaagcac
    16561 acgcaagttt tatcgatgaa ctcgccaaac ttaagaaaga ttacaacgaa ggcggaggca
    16621 acatccttgt gatactcaac gccaacagga tggtcatcaa ctggctcacg aaccatataa
    16681 ggagcatgga caaaaaaata ggcgaatttg tgaaaaatat gtaaaacttt tagccggatc
    16741 cgtcggtgat aactccggga tgacaacggg gccaatatct gaccgataac gggggttgtg
    16801 ctttgtcgcg tcaaccccct gttttcatag atatccatcc tttcatccct ttcatctctc
    16861 ttaatcgtca ggataaagga tgatacgatg tcagagtcaa gacattttac agaaaagtta
    16921 tcgcttctgt tttaccctca aaattgcaga tatgaaataa tgcgtgctgt ttttctgttt
    16981 tcctcccgaa aaataacagt cgtcaacatg ccggaccgtt tctatatcat aatcagcgaa
    17041 cagatccagg atatcgtcaa gcgacacttg ttgtcaatct tctaattccc catcctcagg
    17101 cgagtaggct cccgtcacct cgatcacatt gccgtccgga tcggtcaact gaaaatagta
    17161 atagggactg acattgtaaa tatatttgat gcccgtcaaa tcatcagata atttcaggct
    17221 cctgatcctt tcatgttctt tacgcagatc ttctgtccag aagttcagaa cgaacttgcg
    17281 cgtattcggc gcaagagcga tttttttcag gtcatcgaac tcctcggtat attcgccttt
    17341 acggacgacc ttgtcaggat ggtcggcatc gaaatggccg ttcattatcg aaatattatg
    17401 gccttcaaaa tcaaatgagg caaacctgtc catattcctg tgtgttacag gaattccaag
    17461 cagtttttca tagaaaccga ccgttttttc aaaatcgctg accactaaat aaatcgcacc
    17521 caaatgcata acgttgccac tgctcccttc ttataatcct tactgcatct gatcttccag
    17581 gtactctaca atatcccgcg cctttggcgg gcatcccctg acgcatttgt cgaatcccga
    17641 tgtacatgaa ccgatgcctg tcgcgttaag gcgtttatcc ctgaagccct ggccgatata
    17701 cagtttctcc tccagcctgt tcagcatgcc cttctcacgc agcctttcca gagcatggat
    17761 aaggcttccg taacatgccg agcaggcgca gtcctcggcg atatacgccg caagctgtct
    17821 cactcttcct gccggtatcg atccctgcac tattcccggc atcatccccc tcgatccgct
    17881 gatccttttc gattctgtcg tcccggtttc cctgtccggg acttccctgt tcaattcgac
    17941 tatatctgca ttttccaggt ccgctgtgcc cacgccgata cgttcagcta ttgagatata
    18001 agggatttcc tttatgtcga agcccataag atgcgccgca tatgcatccg tcagcaccgg
    18061 atctttccct gcgaatatcc tgttcatccg gaccgggttg ccgccttctt caaagttgag
    18121 gtctccgttc atgccgtcta cgattatcag atcctgcttt ataattttgt tcaggtatgc
    18181 tatgggtttg tgcagaccca tcgtgtggaa ccgtcttttc tccggatcgg gtatgcagcc
    18241 cttcatgttt ttaagtgcac aggtcatttt ggtctggcag tgtcctttca tgaccggcac
    18301 gtttatcagg taaccgacct tcatcacacg atcgcagacg cttatcctca ttccgtccgt
    18361 ttcatatgtc ctgcttttat ctttctgtag atcgataagc ggcacatcgt atttcctcga
    18421 caaatcctcg tatccacata ctctgaacgc ttcagaagtc ctgtcgccta cccacgagcc
    18481 ttccattatt actatgttgc tgcgccccct cgattccaga tattcgatta tccccgccac
    18541 aatttcgggc gaagtcgtcg cacctgagtc cgaaggtttg gcaacgacaa gattcggttt
    18601 gatgccgata agtgtatcct cagctatttc tgcttcgggc tttatggtat caagcagttc
    18661 tttcaccatt tgcccgggat cgtctccgta tatgatgtgg attccgtttc tgctgatcat
    18721 atcaatcggg tcctttaatt tgatgccttt cagggaattt ctcttaaata tataactcta
    18781 agtatgacag ttctgtttcg aaaagtaaac aacacggtaa acttgacatt gcatactttc
    18841 agacatgctc ttttagcttt atgtactccg aaaatgatgt cgctccaagt tttcgacgtt
    18901 ttgcaagcat tgaagcccaa agagcattcc cttatctatg gcagccaaat cggccaactt
    18961 attattgatt aggtaatcga tacgctgctt caggtgaggc gtagtcggat atgacaattc
    19021 atcatatacg ggccttggaa ttactaccct tcccggatag agcccggcca acaggctttg
    19081 gctgtttacc caaaggaatg cggagataca gtcagtatcg aagaaaagcg aatcagtcat
    19141 tcagttcgcc tccctcgact tcatttccgt aaacgaggtc cggcctgaat gcagtcaata
    19201 ataattgctc atatttgcct gatgaaatta gtcctttttg catcagttcc tcagctgtct
    19261 ggatatagta tccataagtt ctgtaacgct tctccggagg aagcggtttg taaagcgagt
    19321 cgtcaaaccc taagctcact gctgaactga ttactccctg cctgtactga tctgtctgtt
    19381 gaaccgtcaa tattccatcg tcaaccaacc tgaataaaag cgcctgtctg ctcaccccga
    19441 aaaactgctc catcatgacg atttctctga tacccaggct cccttttgaa ttgccgtgta
    19501 tttttctgag cgcctcagtc aaggcaaccg gcggcatcaa aaaataggac gcaaactgat
    19561 cagcagcctt ttcggcatca cttccggtcc caatctttat tgaacagact gttgtccgcg
    19621 cattttcgtc ataatagagg tgataaaact cgtgagccat tgaaaaacgt tgtcttccaa
    19681 cagacatgga cgagtttacg gcaattaccg ggtaaccatc gttctttatg catatcccgc
    19741 tcaggtgatc acccatcgga taaaacacca atgtcagctt atcgatcgaa agagccagtg
    19801 aaaaaatgtc gacgggcgaa gtggcatctt cccctagttc tttccttaaa cttaaagctt
    19861 ttgtgcaaag gtccagttta tttatcaacc ccacagcctc ccagcagtct tgtcataaaa
    19921 ttcagattca gagctatacg atttatgctg cttatcgttt ccaagtcatc atcactgatt
    19981 tcactggccc tgagagcaaa tgatatccgg tccgccggtc gtgtttcctg tgtcagctgt
    20041 tctattgaca ctccaaataa tgcagacagt ttatcaagca tgtctgatgt tacagaccgc
    20101 tcattgtttt caattcttga gatcaaactc tgatcgacat tcaaatattc tgcaaggcat
    20161 ccctgcgtca aaccgctctg ctctctcaaa tactttacat tttcaccgat tctccgggct
    20221 aaaccatcca ctgaacatcc ctcctctgtt tctattatat gcagttcatg tcattatttc
    20281 aacatatatt ctactctttt ttatgacata acaatcacgc ttactaaact ccgaaatgta
    20341 tctatctggg cttaatatca actatatgta agaaacttct ttatctgcct tgcggcggga
    20401 tcagacacaa ggtacgaaat gtcgatccga aatagtgctc aaagagtatc tcctcgtcat
    20461 tactccatgt tgtacaatac catgatcaac tggctgatga ccgtcggaac ggcaagcgac
    20521 aataccgcct ttgtcacagg agctttttca aagagttctt tttccctgtt atccatcata
    20581 taagccttct tctattattc agcaaagtca cgttcacatc aattttaata aacctgcagc
    20641 aaaaatgaaa gtgtataaaa acagccggct gtgtttctgc atcccgtgag gggacaggac
    20701 tgcagaaaac ggccggctgt ttgtaccggc atctgaatat tataatgaca ctgctttcct
    20761 gcttttcggg ttttcagccc acagcctgtc ggcgacttcc gcccaggctt tcgcagccgg
    20821 agtggttttt ccgcaaagca cctcgacatc ttcaggcgct ttcatatcag ttgattttgc
    20881 gcccgattct ttgaccattt ctttaagcat ctcaggattg tcaagcagcg ggcacgggcg
    20941 cagcatgttg ctgttgaacg gctggcgcct gcggtaagcc ataaatatag gagaacggag
    21001 cgcatccagg agcgacacgt cgtgtatgtt gacgttggaa taatggatga atgcgcacgg
    21061 ctcgacatcc ccggccgcat tgatatgcag ataccgtttt ccgcctgcta tgcatccgtc
    21121 ggcgtactcg ccgtcattcc agaaatccat tgcgaaaatg ggcttcgttg cacggatctc
    21181 atggatacgt ttatacatat atgcacgctg ttccggagta gctatgagat ctgtatgcgc
    21241 atccttcccg acaggcatgt aggtgaagtt ccatgagaag cggcatccgc gggcgatcat
    21301 gtcatcgacg aattcatcag agccgacgct gtcagtatta tagcgatggt aacaggtgga
    21361 gtatccgaac accagcctgt gtttacgcat gcgttccatc gcttcgataa ctttgcggta
    21421 tgtgcctttg ccccgcctca tatcagtggc ttcctcgtca ccttcgatgg aaaatgcaag
    21481 agcaaagttg gctacgcgca gcatctcctc acaaagttcg tcatccacca gcgtgccgtt
    21541 ggtgaatgca aaaaagtagc agtcctggtg ttcactgcac agcctgatga tgtccttttt
    21601 gcgtacaaga ggttctccgc cggagaatat gtagaagtag atgcccagtt ctttaccctg
    21661 ccggcagatg gaatccagca tctcatagga caggttgttc ttgttcccgt attgtgcggc
    21721 ccagcatcct gtgcaatgca gattgcatgc gctggtagga tccatcagga tggcccacgg
    21781 aacattgcag tcatactttt cttctatttc gcatgtacgc tcaaaacctg taaggtgggc
    21841 gttgatcatg aagcactcga caaacttctg tatgcacttt ttatccacct catcgtacag
    21901 tttctttacg aaggtatacc agttgctgtt cctgtccagc aaagccttgc gcacaccgtc
    21961 tatctgcaca gtatgcagat tccccctgtc cagcttttcc gcaatgtcga atatctttgg
    22021 cagtttcgcc agaggatcgt ctgaaatgat atagtccagt aacatattcg ctgcagcttt
    22081 cttaatttta ttcatcattt atccccctta taccagatag attttgtttg aaagtctgta
    22141 gtattttaac taaccattcg gttaatagtt taactaaccg tccggttaga tttgctcata
    22201 tcagattaca ggcaatataa agcacaggtt cttctgaata tcattttact ttgaaaaatc
    22261 aggcgtcagg gccgtggctt ctcagcaaga tatcacttcc tgaaatcaat gatttgttga
    22321 ttatgtgata aacccgcatg aaagatctgc gaagttcggc gtcgctcagt gagcttacgg
    22381 ctttatagtc gtcaaaatag gcagcgaaac tgacaatagg tataattgaa aagaaaaatt
    22441 tgaacagtac catttcctcc gagtaaacaa aatcctggtc cgcgctggat attgttttga
    22501 aaaacggatt ctcatcattg ccgctcatga atttcatgaa ccggaacaat gaattctggt
    22561 gtttgccctt tttgagcgat tcgagcacca tgatgcggat gatctccttt ttcgcaataa
    22621 catagtcgat tacccgctca tagaacttat atgagttttg caggaaagca tttatcgctt
    22681 cgtcattagc gaagtgcaaa cggtcgggct ttatatccag gtggccgtcc cttaccattt
    22741 gaacgatatt tgtctggata tagtcaagca tgcccgacac cgcatcgtca agaatggaat
    22801 cgaccatgaa atccagaatg tcctgcttgt ttttgaaata ataatatatg agtgctttgt
    22861 tcacattagc tgcgcccgct atatcgttta cggtcgtcgc atcatagcct ttcctggaaa
    22921 aaagctctat cgcggcattg attattcttt ccctggcttc gctcccggtc gttttctcca
    22981 tcatatgcct ccggcaattt aattaaccgg ttggttaatt ggtttaatat aatattatat
    23041 ttgtcaattt ccattgtcaa gaggtttcaa accatatttg tttcacggcg tatagcagac
    23101 agcatttcat gccgtatgtc cggataaagc cattcgtctc ttttacagta tctgttttaa
    23161 gaccgatccc ttaccgctga tagatgcttc cgtaaagaga atgatcactt atagaatgta
    23221 gtcgaacgaa gtttactaat gaaatgaaag ccattttatt gcttccctgt gaaaatacct
    23281 tatgacgaac gctttttcac aaaggatccg tcttgatata caaattgctc aggtttattt
    23341 ggctcaaaat aaacttcgaa aagcttattg ttctcatcca ttagacgaac tgattttgca
    23401 cccagcgatc tattaatgtc agaaaccgat atttctgaat ctttcagtac atatatattc
    23461 gtttcgggaa tcgttccgac ggttgctacg atctcattgc cggtatcgtt gtcgagatcg
    23521 gtttccgtcg tgttaccgtc tatttgaata accgaatctt ccgggttctc tccggtcagc
    23581 cagtataaag cctgcggata gtttgccccc aggatcccat aaaactttac cgctttcttt
    23641 ccgaacacct ggacttcttt gatccccatc agatctccag gcgttccctc catcgacacc
    23701 aggccaacat aatatgactt tccgccggta ctgacagcgc ctttatattc accgtcttca
    23761 tcgtcagtaa agatatacgc gctcagatcc tgatcaatag ttttttcctc aataatattc
    23821 acactgctca agtctaaagt ctctgtttcc gccctgtccg cctcggagaa cttcagccga
    23881 tgggcattct cgtcatgagg cgtcccgaag cttgcggcaa accaaaccgc aactgcccct
    23941 gcggctgccg ccagacaaat gatgatccaa aacaccggca ttctgatgac tttgcccaaa
    24001 atcgcacctc cctgcgcccg gtcacagcaa aaccgcaggc tggctcatgc agcctgcagt
    24061 cagagtttta tcaccttcgg atcttcggta aaggaataga acacggctgt cgcatcctta
    24121 aagtaaagga cctcggtgtt gtcgtcttca ggcgaatata aacgtttcaa ctccggatag
    24181 ttgtcaagca tatgcttttt agcctcgatt ctgtcatccc tgataagttt gcctgtcacc
    24241 ctaagccact tcccatcggc ccatgcacat atctcagcct tcggattcgc ctggagctgc
    24301 tttgatacgt tcttcacctt acctgtctgg atgtaaagtt tgccctcgaa aatatccact
    24361 gtgccaaacg gcctgaccct cggctgatcg ccttccaccg tagccaggta atacactccg
    24421 catttcttga gaaactcata gacttcctgc atgttacttg cctccttaat gtttattagg
    24481 cggggagaca gttaccttat atgatcccct gtcccccttt caataatata agttttcttt
    24541 aattttttcc ataagttctg tgccgcaaac ctctttcagg atgtataaag ctctttttat
    24601 gccggtcagg atacccgccg ttataacaag attcccgttg aatatggacc gcaaaaatcg
    24661 agaacttcaa catcatcgta aatatggatc ccaacgttca gcatgcagcc ttcccctcac
    24721 tgtaaaactt tccgccacgg cagaagcgtc ctgccgggtc accggatact tgcaatatca
    24781 gatggaagcg gtcctgcatt cataccggta tataacatct acacaatcat gcagccatct
    24841 gtatcaggta ata
//
LOCUS       DAOSCC010000008        24212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1506_length_24212_cov_4.691849, whole genome shotgun sequence.
ACCESSION   DAOSCC010000008 DAOSCC010000000
VERSION     DAOSCC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24212
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_24212_cov_4.691849"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1184
                     /locus_tag="PLG72_01105"
     CDS             129..1184
                     /locus_tag="PLG72_01105"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOJ79433.1"
                     /translation="MRRLELSQLKPGMRLAKDVILPDGRLLLLAGFIVKPLYLRKLEA
                     FNVDYVYVEDGEYEPFEEFEEEKLYNHAAATIKNIFTLARRDRDVDIISVMDTVNDIL
                     NKVMQNETVMLQLTGIRDIDNYTFLHSVDVCIYSIIIGKKLGYGREPLMTLGMGAILH
                     DIGKCKVPIEILQKPDRLTDDEFHTMKLHTVYGYEIIKNSYQLSTKTANIAFQHHEKW
                     DGSGYPMGISDNEIDPLSRIVALADVYDALTSDRVYKKRELPHVAAEYIKNNAGVLFD
                     PYIVDLFVNSIAVYTEGTLVLLSTGEVGSVVSSGSVGGARQKISVFSNKSGPPVLQPY
                     IVDLNERTDVEIVEVFM"
     gene            complement(1251..2444)
                     /gene="metK"
                     /locus_tag="PLG72_01110"
     CDS             complement(1251..2444)
                     /gene="metK"
                     /locus_tag="PLG72_01110"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ79434.1"
                     /translation="MHRKYFTSESVTEGHPDKICDQISDAVLDAYYEKDPYARVACET
                     AVTTGMVLVIGEITSKYSVDIPKVVRQTILDIGYNDSKLGFDGNSCAVLVSIDEQSPD
                     IAMGVDKALEAKNGQMNDSQIEAIGAGDQGMMFGFACNETPELMPMPVSLAHRLTRKL
                     AEVRKNGTIAYLRPDGKSQVTVEYDGDKPVRIDTVVISTQHEEGVSHEKVEQDVIEHV
                     IRRVIPSGLIDNNTKFLINPTGRFVTGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNLVAAGLASKLEIQLAYAIGVANPVSVSVDTFGTGKIPE
                     GKIIELVRKHFDLRPAAIIRDLGLRRPIYRQTAAYGHFGRTDIDLPWERTDKAEILRS
                     DAQYI"
     gene            complement(2501..>2824)
                     /locus_tag="PLG72_01115"
     CDS             complement(2501..>2824)
                     /locus_tag="PLG72_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOJ79435.1"
                     /translation="AANDLKQANSIARSMIMKFGMSETLGNMVFDENDEVFIGRDFGH
                     TKNYSEEMAAIIDKEIKRIIDTAYEKTKSILSEHINVLHKVAEALLEKEKLEGYEFEA
                     IFAGT"
     assembly_gap    2825..2834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2835..4289)
                     /gene="ftsH"
                     /locus_tag="PLG72_01120"
     CDS             complement(<2835..4289)
                     /gene="ftsH"
                     /locus_tag="PLG72_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ79436.1"
                     /translation="MKYFKGLSFYIVIFVLVLFLLMIYTNSGNPVQLSYTQLLAQIEN
                     SNVQSIVLKGDEATVELITPNKGSKTKEYVVYISSVDSFTDMVTEAWKNGQVKDFRVQ
                     KPPTAPWWVSILPTLGLIVIFVIFWVFFLQQSQAGGGNRVMSFGKSRARLNTDEKRKV
                     TFDDVAGADEEKEELKEIVEFLKSPKKFIELGARIPKGVLLVGPPGTGKTLLAKAVSG
                     EAGVPFFSTSGSDFVEMFVGVGASRVRDLFDQAKKNAPCIVFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNEGVIILAATNRPDILDPALLRPGRFDRRIYIGLPD
                     IKGREAILKVHARGKPLGSDVKLDELAKSTPGFTGADLENLLNEAALLAARKNKRRIE
                     MEEIKEATFKVVVGPEKKSKVMSEKEKRLTAYHEAGHAIAVKVASSTNKVDRVSIIPS
                     GRAGGYTAHKPDEDLSYHTRSQLMEEIIIAMGGRA"
     gene            complement(4425..4970)
                     /gene="hpt"
                     /locus_tag="PLG72_01125"
     CDS             complement(4425..4970)
                     /gene="hpt"
                     /locus_tag="PLG72_01125"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ79437.1"
                     /translation="MMKDIEEVLVPREKIREMVKRLGKRISEDYEGEELVLVGVLKGG
                     FIFLADLMREITIPVDMDFIAVSSYGASTKSSGVVRIIKDIDFDIAGKHVLIVEDLVD
                     TGLTLRHLKDLFNTRSPKSVKICTAFDKPSRRKVEIEVEYNGIEVPDKFIVGYGLDYA
                     GKYRNLPDVCTLRQDVYEKKH"
     gene            complement(4954..6372)
                     /gene="tilS"
                     /locus_tag="PLG72_01130"
     CDS             complement(4954..6372)
                     /gene="tilS"
                     /locus_tag="PLG72_01130"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ79438.1"
                     /translation="MDIILANRLIEYGEGVVAGISGGPDSVCLIHVLSSLRPELGMKL
                     YAVHINHMLRGEEADADEEYTARLCSELGIPLHVMRTDVAAAAAKMGISVEEAGREIR
                     YREFGSYAGTVGASKIAVAHNRNDQAETVMLNIIRGTGTRGLCGMEYIRGNIIRPLLS
                     TDRDEIERYCREAGLSPRTDSTNLTGEYTRNKVRLGLFPYINENFGANIVNSLCRLAE
                     NAAADRCFMERCAAEAYERVLREKGDGQVSMDIGRLRELDTAILMRVLRLAVIHASGN
                     AKGVGGVHYQALTELVMKGATGMKAELPRGIRASVSYGILKVSCGESAAGKERHPGEF
                     SREVSIPGTTEVPEAGAELSASVHPALSIDKYKSLGYNPYVQYFDYDLLKKGIHIRNR
                     RAGDIFRPFGSGGTKKLKEFFIDSKIPREARGRIPLICRGNEIVWVIGYKISDKFKVT
                     ENTKKVLKIAFNRRGHDYDEGY"
     gene            complement(6401..7744)
                     /gene="dnaB"
                     /locus_tag="PLG72_01135"
     CDS             complement(6401..7744)
                     /gene="dnaB"
                     /locus_tag="PLG72_01135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ79439.1"
                     /translation="MDLSALGMVPPHNIEAEQAVLGCMLLDSDVIPTVTELIRSSDFY
                     REDHREICEAIIDIVEKAGPVDIITVAEQLQQRGTLEKVGGIDYLASITSAVPTTANA
                     RHYAKIVEEKSLLRKLIKASQEIAGMSYEGAEEAEFVLDKAEKTIFDIIERRSTQGFT
                     HIKDVLLETFNRLEELYNSKSPITGVPTGFTDLDMKTAGLQNSDLILIAARPGMGKTA
                     MALNIAQYAAVQKHVPVALFNLEMSKDQLVNRMLCSEVMVDSHKMRTGKLDDEDWKKI
                     AKALGPLSEAPIYIDDTPGLTVMDIRAKCRRLKLEKKLGLVVIDYLQLMRGRGKSENR
                     QQEVSEISRSLKILAKELNVPVVTMSQLSRGPESRTDHRPMLSDLRESGAIEQDADIV
                     MFLYRDDYYNPDSEKKNIAEVIIAKHRNGSTGTVYLKWFDKYTKFANLEKDGTYQ"
     gene            complement(7847..8293)
                     /gene="rplI"
                     /locus_tag="PLG72_01140"
     CDS             complement(7847..8293)
                     /gene="rplI"
                     /locus_tag="PLG72_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ79440.1"
                     /translation="MKVILKQDVKGLGKKDDLVNVSDGYARNFLFPRGLAVEANESNL
                     NIVKTRKEAEKSKKDRELAQARKLAEELRNVEVVIRAKTGENGKLFGSITSKDISDKL
                     KEDFRLDIDRKKIVMPDAIKSTGAYELDVKLYPEVSGRLKVRIESE"
     gene            complement(8307..10325)
                     /locus_tag="PLG72_01145"
     CDS             complement(8307..10325)
                     /locus_tag="PLG72_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ79441.1"
                     /translation="MDSKKILSTLIPQVSIHIWIILMLTVVIAVLDWMVAIPCFILFV
                     YLAWHNIRTNYRRKAEISEFIENLNLNIDTATKDTLLNFPMPLVISEPDGSIIWYNSS
                     FRDISEHTEIFENAVKSVVKELRSELPVDNSDRKYMPISRQININGRHYNVLISIARR
                     DQQDITNCILILYFIDNTELVNLKQTYADERIVAGICVIDNYEDLMQSMEDPLKPQIL
                     AEIDKRVVKWISFTNGIIKKYERDRYLFIFEQKYMKDLEEKKFEILDSVKEINIGNKL
                     PVTLSLGFGLNGGTLAENLGFAAASIDLALGRGGDQVVLKNGENFSFFGGKSRELEKR
                     TKVKARVIAYALRELIDQADRVLIMGHENGDIDSLGASLGLYRVVRSRSKDAYIVLNK
                     INPTIEKLAEMIRKDEEYADLFLTGNEAIDLVTRKTLLIVVDNHRRSFAEVPELIDES
                     EQTVVIDHHRRGADYIQDTVLTYQEPYASSTSELVTEVVQYIDEKLKLNQIEAEALYA
                     GIVVDTKNFTFKTGVRTFEAASYLRRQGVDTVAVKRLFQNDIETYSKISAVVSGAELI
                     EGDIAISVCGEDIDNPQLIAAKAADELLNLSGIAASFVLCSVGGEVWISGRSLGEINV
                     QMILEKLGGGGHMSVAGAQLPGTDIKDAKERLKYAIMNYKEEMAKPNT"
     gene            complement(10345..10683)
                     /locus_tag="PLG72_01150"
     CDS             complement(10345..10683)
                     /locus_tag="PLG72_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOJ79442.1"
                     /translation="MALPEKEIDITRNIKIIECLKSEMLTEVADLFKVLAEGAREELH
                     EAVGDVLSNVILISYLLGKRLGVSYNAVEMKIRSKVRLGLVENNDVEKYYGDLSELSK
                     HLGSSRRKEK"
     gene            complement(10741..11577)
                     /gene="pheA"
                     /locus_tag="PLG72_01155"
     CDS             complement(10741..11577)
                     /gene="pheA"
                     /locus_tag="PLG72_01155"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOJ79443.1"
                     /translation="MKRIGYLGPRGTFSHEAMVKYTGEVPYISREYPSIPDVFAAMLD
                     GQLDEAIVPIENSIEGAVNATMDMMTQDNGLWIRAEVVIPVREHLLVKKGTGISEIRT
                     VISHPQPLGQCRRFLAENLPGAVLKAAHSTAAAAEEVAAGTGGMAAIGSRAAAEVYGL
                     EILREDIQDHDSNFTRFIVLGGSDAEKTGNDKTSLIFSTEDKPGSLYRILQIFNLWDI
                     NMTRIESRPARSGLGRYIFFVDVLGHREDEDLRDALTMVKRKTSFLKILGSYPVSPVD
                     KI"
     gene            complement(11764..12048)
                     /gene="rpsR"
                     /locus_tag="PLG72_01160"
     CDS             complement(11764..12048)
                     /gene="rpsR"
                     /locus_tag="PLG72_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ79444.1"
                     /translation="MTAKKDNKSANKDYDKGEGKVNIKRRSRKKVCAFCVDKVTEIDY
                     KDVAKLRKYISERGKILPRRISGNCAKHQRQLTTAIKRARTIALLPFTAD"
     gene            complement(12122..12562)
                     /locus_tag="PLG72_01165"
     CDS             complement(12122..12562)
                     /locus_tag="PLG72_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ79445.1"
                     /translation="MNKVILMGRLTKDPELRYTASSNIPRCSFAIAVDRRFSKPGEEK
                     QTDFFNIVAWRSTAEFCSKYFAKGQRVVVVGSLQNRSWDDNEGKKHYVTEIVADEVYF
                     ADSRRTEGGSAYGPTGASGFVPASAEGSDGDAGFFPTDDDDLPF"
     gene            complement(12572..12856)
                     /gene="rpsF"
                     /locus_tag="PLG72_01170"
     CDS             complement(12572..12856)
                     /gene="rpsF"
                     /locus_tag="PLG72_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ79446.1"
                     /translation="MLNKYECIFIIDPDLDEENTKALVEKFKNLLETSAQLEGIDEWG
                     KRKLAYEIADKTEGYYVLADFSANSDFPRELERILKITDGVLKYMIIKKK"
     gene            complement(13028..13372)
                     /locus_tag="PLG72_01175"
     CDS             complement(13028..13372)
                     /locus_tag="PLG72_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79447.1"
                     /translation="MVAGGSRRIVVIKNIASNLIEEAILILRGGPDEPADAGGEKDGQ
                     CRPEPKDDFLLREAEHIINEYIKEHGLTKKREKRLPPGRLFRSGMSVDSVVNILLAAA
                     VLLLLAIIFKMM"
     gene            complement(13507..15879)
                     /gene="spoIIE"
                     /locus_tag="PLG72_01180"
     CDS             complement(13507..15879)
                     /gene="spoIIE"
                     /locus_tag="PLG72_01180"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HOJ79448.1"
                     /translation="MKITAVQMSKPGYLPKLINRLYFQRNRILLFLTAFLITGSSISA
                     AVPLGAVFFAASCGAGVPRLMTAAAVMLGAFILGPFELVYINAACMLLFFVLSIPLKK
                     TDSKMDIWAAFAAFIAILVPQLILSGIWGFLLYDVLKSLFCSFISFVFYFIFRFSIPV
                     ISGMAGKAALDGEEAVSAGITAALAVSGFGALQLFGFSLKNILCVLLVLFYSFKCSAG
                     AGAATGAAAGLIVSASKGFTPSTAGAYALCGMLAGILGSLGKAGSALGFVLGNMILAI
                     YFNGPAESMLYLRDILAASVVFFIMPDRLMDRFTAPFARKSAEHADMKSYSRRIRDLT
                     AQRLRKFADAFMALSRSFGEMARNSAAAGKHDINVLFDRVADRICRDCSLCLHCWERS
                     FYDTYQVMFRIVESLEARGRVEESDIPADFIDKCPRINDFVNAVNNMYELFKVGVVWK
                     SKIGESRSVISRQFEGMSRVIGNLAEEINTDISFMSPLEDAISTALKKEGVRVVEVVA
                     YKGLWGKYEINVRHSSCGGARKCTAAIDRAVSETTGRRMVRSDEGCTSYGSEECILKY
                     MEAENLRITTGVARMPKHGSRVSGDSFSFMEGISGKYTLALSDGMGTGHSASAQSKAA
                     VDMLESLLESGFDKDMAVSLINSVLVLKSDEDSTCTMDIAMIDLTSGEVEFIKIGAAP
                     TFIKRDSRVEVIRSASLPAGILPGVDAELSRKIMDAGDMIIMVTDGVTDAMAGDEPGD
                     RSLLKHIRQLESLNPQLVAESILDEAVRRCDGKPYDDMTVLVAKVWKKPL"
     gene            complement(16014..17909)
                     /locus_tag="PLG72_01185"
     CDS             complement(16014..17909)
                     /locus_tag="PLG72_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HOJ79449.1"
                     /translation="MKGIVMEITGKYAVILTQDGQFRKIKAQPGIAAGAETDTGMPSL
                     PSAARKPVYRIASIAASFLLALGIGIFSYSMPYSYVDFDINPSIGLTANIYDRIIRVE
                     ALNDDGSKLIENKNLMHRRLDEGVTLLLSSAVEQGYLKENTGSRCKSEAGGTGRQDAA
                     DVQDGTSAPQDTSDHTLTPGGGQTPENGAETADSGSDAGYSGSAAEGSGGNAGSGKSD
                     PKGTAKPSAKTEGDTGKQKEKPSGGSGKLQNAVILTVSSSNAKKADELKKIVEDMAAR
                     ELNKDNVESKILVGETSVKQREAAHKLGVTPGKLALIEEVSGYFPLADFEKMKNTAVK
                     DLLEMVREKKEQLAKGSAGKKEDKEKKEDKAKEGKPGANAAAGGSNSNKDNKVIKDNK
                     DNKNSSAATGTGIGISTNGSNAGTIWPWPGGSNTGGQNSGPKEDKGGGGIKPGAGAGK
                     VTGKKDIDDSRKDKNAGVQSGGGNHGFSGLPGIGNPRQNDIKQPGEGNGKLDSKNSNK
                     GIKDAKPDLLSWDDVRKTLEKQGEKLQEERERLRNEFLDQISKDKAKIGNTIDTPRGK
                     RPDAGKDAADNKKQDIRDSSKKDERDSKNENEKNDTRKNDKNDKNGKNDKSPGLNIGP
                     KDNRKKR"
     gene            complement(17925..>18335)
                     /locus_tag="PLG72_01190"
     CDS             complement(17925..>18335)
                     /locus_tag="PLG72_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HOJ79450.1"
                     /translation="VVPLNVFAEEKETEYDLGADEAIREFSDRDIAELRKAEIQELAK
                     ELSQWNITYRDLAKVSPRHRRTREQCAGLAGIILSRPDMLESVMTKKYLPVMELEKAS
                     GLPRKFIERFRKYIIAIVVIAIGDYQYIKDYIKL"
     assembly_gap    18336..18345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18346..18623)
                     /locus_tag="PLG72_01195"
     CDS             complement(<18346..18623)
                     /locus_tag="PLG72_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ79451.1"
                     /translation="MQFDAINARISSIRDDAEETGRFVEVYKPFIASCAEKVTGRYMS
                     YDTDDELSIAMIAFVEAIRSFDSSKGNFFTFSRNVIKRRLIDYYRKERR"
     gene            complement(18857..19411)
                     /gene="spoVT"
                     /locus_tag="PLG72_01200"
     CDS             complement(18857..19411)
                     /gene="spoVT"
                     /locus_tag="PLG72_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOJ79452.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDKEGEV
                     ILKKYSPIGELGDFAAQYAESIHKTSGHITCIADRDTIIAVAGASKKEFLDKSLSPEL
                     EKVIEEKTTFVAKSAEDKSISILADEGNERKYVAQVVSPIIAEGDPIGAVIILSTDPG
                     SRMGEVEAKLAQSAAGFLGRQMEQ"
     gene            complement(19560..20681)
                     /locus_tag="PLG72_01205"
     CDS             complement(19560..20681)
                     /locus_tag="PLG72_01205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ79453.1"
                     /translation="MEDKKTKKSSGGDYAGEKTGMPLFVKYGLIAGAIVLAIAIGLII
                     YFNTAERIVATVGGEKITETEFRYYLDYQKQLMFYSAQQADPNITEETFWATKIGGED
                     PVEVAKKQILEELKSLKVQYRMAKEANVELTRDEKDYIDLLTEKQIIEPYGNGNRIKA
                     NKAFKENYGISIDEYEKIQEQIFIVQKYQTEETGKITDADIEKYYAENTDLYKADTKY
                     RYDAEEAVWAKHILISVDKDASDEDWDAALEKAKGLIEKLKAGEDFATLAKENSDDGS
                     AQWGGDYLFGKDRMVDEFEKAAFELEPGEFTEEPVKTEYGYHIIKLEEKYAEGEPVSL
                     RCAKEYQEYGAAFIYEQRIKELVDKAEYKIENSVYDSIK"
     gene            complement(20768..>24212)
                     /gene="mfd"
                     /locus_tag="PLG72_01210"
     CDS             complement(20768..>24212)
                     /gene="mfd"
                     /locus_tag="PLG72_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ79454.1"
                     /translation="AAVRESKHPVSIIGPSESQKAHISYALCRHCGRKGVFVAFNEMQ
                     ARRLFEDFSFFIGDDVIFFPTKEIMLYDVEAKSYDTVFQRLAALDRISRGDYSFVVTS
                     VEAVSHKLLHPGKLVQNVVTVGDHGRLDPEELIRTLAVSAYERVEFVEKKGQFAVRGG
                     IVDIFPVNADNPVRIEFFGDEIDSLRWFDVNTQRSIDHAESVRILPARELIFDIARRS
                     EIIRSVKSDAEKTGKRDPDLVRKINADMERLEESWYFPGIDRYIPYIIDEPCSLADYL
                     GDDTLIFMDEPARQEQRLENLLLEHEEICKALIEKGSLLPQSTGMYFDYESIFSAVSA
                     RKPIYMNTVNADSIASKGSISYSIPCRMIGSFQGSMDILAENLSMWKRSKNRVLLLSG
                     TRGRGERLRDSLSEKGIESVYMDDVTRPVMPGQVVITRGSLHRGFEYPTIGFVVISDD
                     EIFGRDRKKTARSQGKYKGRPISLFTELAVGDYVVHQTHGIGQYVGLEKLTVEGIKKD
                     YLKIRYQDGAYLYIPTNQMDLIQKYIGTEGKQPKISKLGGSDWLKTKTRVKESLKKLA
                     EELIKLYARRAALKGFEYSKDTVWQRQFEEQFPYEETEDQLRCIDEIKQDMESGKVMD
                     RLLCGDVGFGKTEVAIRAVFKAVMDGRQVAYLVPTTVLAQQHYNNFAERMKDFPVTVE
                     MLSRFRTRAEQKRILRDVKNGKVDVLIGTHRLLQKDIRFKDLGLLVIDEEQRFGVSHK
                     ERLKNLAPEVDVLTLTATPIPRTLHMSLTGIRDISVIEDPPEERYPVQTYVMEYNRDV
                     IRDAIIRELARKGQVFYLYNRVRTIDTKAAEVQDMVPDARVAVAHGQMDERDLEDIMM
                     SFIKGEFDILVCTTIIESGLDMPNVNTIIVEDADKMGLAQLYQLRGRVGRSNRLAYAY
                     ITYRKDKIISEVAEKRLQAIKEFTELGSGFRIAMRDMEIRGAGNLLGPEQHGHMESVG
                     YDMYCRLLDEAVRELRGEPPRKEEAEVTIDINIDAYIDDSYIGNESRKIEMYKKIAAI
                     SDENDVIDIEDELTDRYGEIPVPVRNLVKVAHIKALARSLGISSVTEKKDSVIFAFAD
                     SKSINFQAIAKIAEKYKRQLLFSAGASPYLVYRKPQISADSLADNIKILLQDLKSYDT
                     G"
BASE COUNT         5032 a         6827 c         5349 g         6984 t
ORIGIN      
        1 tatataatat gcaccatata tcgaaatgtt acctggactc tggttaatcg tatggcatta
       61 tgttaaataa tatgtcacat tgtggtataa taacatcaca tacagtacgt agatcgggag
      121 gatattgtgt gcgtaggctg gaactctctc aactgaagcc cggtatgcgg ctggcaaaag
      181 acgtgatcct tccggacggc aggcttctgc tgctggccgg ctttattgtc aaaccgctgt
      241 atctcaggaa acttgaggca tttaacgttg actatgttta tgtagaagat ggcgaatacg
      301 aaccctttga ggagttcgag gaagaaaaac tttacaacca tgctgcagcc acaataaaga
      361 acatctttac actggcaaga agggacaggg atgtcgatat catttccgtc atggatacgg
      421 taaacgacat acttaacaag gtgatgcaaa acgagaccgt gatgctgcag cttacaggga
      481 taagggacat cgataactat acgttcctgc attcggtaga tgtatgtata tactcgatca
      541 taatcggcaa aaaactcgga tacggaagag agccgctgat gacgctcggt atgggagcga
      601 tcctgcatga catcggcaaa tgcaaagtcc cgatcgagat cctccagaag cctgacaggc
      661 tgaccgacga tgaattccat acaatgaagc ttcataccgt atatggctat gaaataataa
      721 agaactccta ccagctcagt acaaagaccg caaatatcgc tttccagcat catgagaaat
      781 gggacggcag cgggtatccg atgggcatat cggacaatga gatcgatcca ttgtccagga
      841 tcgtcgcact ggctgacgta tatgatgccc tcacatccga cagggtctac aagaagaggg
      901 agttgccgca tgtggcggct gaatacataa agaacaatgc cggcgttctt ttcgatcctt
      961 acattgtcga cctgtttgtc aacagtatag cggtatacac ggaaggcact ctcgtgctcc
     1021 tcagcacagg cgaagtcgga agcgtcgtgt cctcgggaag tgtcggagga gcaagacaaa
     1081 agatcagcgt cttctcgaac aaatccggtc cgccggtact gcagccgtat atcgttgatc
     1141 tgaacgagcg caccgacgtg gagatcgtcg aggtattcat gtgatccggt acatccgtgt
     1201 gatccgctga tctgtgttat ttcattatta tccggttata tcgatgcggt ttatatgtat
     1261 tgggcatcgc tcctgagtat ttccgccttg tccgtccttt cccacggcag gtctatatcg
     1321 gtgcgtccga aatgtccgta agcagctgtc tgcctgtaga tgggcctcct gagccccagg
     1381 tcccttatga tggcggcagg cctcagatcg aagtgtttcc gtacaagttc gatgattttc
     1441 ccctcaggta ttttcccggt gccgaaggta tcgacggata ccgatacagg attcgcaact
     1501 cctatcgcat aagccagctg gatctccagc ttgcttgcca aacctgcggc tacaaggttt
     1561 ttagccacat agcgggcggc atacgcagcg gaacggtcga ccttagtcgg gtctttccct
     1621 gagaaagctc ctccgccgtg tcttgcatat cctccgtatg tatcaacgat gattttcctt
     1681 ccggtcagcc cggaatcacc ctgggggcct cctgtgacaa acctgcccgt cgggtttatc
     1741 aggaatttcg tattattgtc gatcagtccg gacgggatca ccctcctgat gacatgctct
     1801 ataacatcct gttcgacctt ctcatgggaa accccttctt catgctgtgt ggaaataacc
     1861 acggtatcga tccttacagg cttgtccccg tcatactcca cagtcacctg ggatttgccg
     1921 tcgggcctca ggtaggcgat cgtaccgttc ttgcggacct cggcaagctt cctcgtaagt
     1981 ctgtgtgcca atgaaacagg cataggcatc agttccggag tctcattgca cgcaaaaccg
     2041 aacatcatcc cctggtcgcc ggctccaatt gcctcgatct gcgagtcatt catctggccg
     2101 ttctttgctt ccagcgcctt gtccactccc atggcgatgt caggggactg ttcgtcgatg
     2161 gaaaccagta ctgcacagct gttaccgtca aatcccagct ttgaatcgtt gtatcctata
     2221 tcaagtatcg tttgcctgac aactttcggt atatcgacac tgtacttcga agttatctct
     2281 cctataacga ggaccattcc ggtagttact gccgtctcgc atgcgaccct ggcgtaagga
     2341 tccttctcat aataggcgtc tagaacagca tcggatatct ggtcgcagat cttgtccgga
     2401 tgtccttcgg ttacggattc tgaagtaaag tattttctat gcatttgcac ctctcctttc
     2461 accaacagaa aaaaacatgc gaaataccat gtacctggta tcaggtacct gcaaagatcg
     2521 cctcgaattc gtacccttcc agtttctctt tttccagcag cgcttccgct accttgtgga
     2581 gcacgtttat atgttcgctg agtatggatt tcgtcttttc ataggcagta tcgattatac
     2641 gcttgatctc cttgtcgatg atggctgcca tttcctcgct gtaattcttg gtatggccga
     2701 agtcgcgtcc gatgaatacc tcgtcgttct cgtcgaagac catgttgccc agggtttcgc
     2761 tcatgccgaa cttcattatc atactcctgg ctatgctgtt ggcctgcttg aggtcattgg
     2821 ccgcnnnnnn nnnncgcacg cccgcccatt gcgatgatga tctcttccat gagctgggac
     2881 ctggtatgat aggacaggtc ttcatcgggc ttatgcgctg tatagcctcc tgcgcgcccc
     2941 gatggtatta tcgacaccct gtctaccttg ttggtggagg aagcgacctt tacagctatg
     3001 gcatgacccg cttcatgata agccgtaagt ctcttctcct tttcgctcat gaccttgctc
     3061 ttcttctcgg gaccgacgac aaccttgaac gtagcttcct ttatctcttc catttcaatg
     3121 cgtctcttgt tcttgcgtgc cgcaagcagc gccgcttcat tgagcaggtt ctcgagatcc
     3181 gcgcccgtga acccaggagt gctcttcgca agctcatcga gcttcacatc cgatccaagc
     3241 ggcttgcccc tggcatgtac tttcagtatg gcttcacgcc cttttatatc aggcaggcct
     3301 atatatatcc tcctgtcgaa ccttcccggc ctgagcagtg cagggtccag tatgtcgggc
     3361 ctgttggtgg ctgccaggat gatgacgcct tcatttacac caaaaccgtc catttcgaca
     3421 agcaactggt tcagcgtctg ttcacgctca tcatggcctc cgcccagtcc ggctcccctg
     3481 tgtctgccca ctgcatctat ctcatctatg aacactatgc acggagcatt ctttttggcc
     3541 tggtcgaaaa gatcacggac ccttgatgcg ccgacgccta cgaacatctc tacaaagtcc
     3601 gatccgcttg tgctgaagaa cggaactccc gcttcgccgg aaacggcttt tgccagcaga
     3661 gtcttacctg taccgggcgg tcctaccagc aatacgccct tggggatcct tgcgcccagt
     3721 tctatgaatt tcttcgggga cttgaggaat tcaacgatct ccttcagttc ttccttttcc
     3781 tcgtcggctc cggccacatc atcgaatgtg accttcctct tttcatcggt attgagcctg
     3841 gcgcggcttt tcccgaagga catgaccctg ttgccgcctc cggcctgcga ctgctgcagg
     3901 aagaataccc agaatatcac gaaaatgact atcaggccaa gtgtgggaag gatcgatacc
     3961 caccagggcg ccgtcggcgg cttttgtacc ctgaaatcct tgacctgtcc gttcttccat
     4021 gcctcagtca ccatatcggt gaatgagtcc actgatgata tatagaccac atactccttg
     4081 gtttttgatc ccttgttcgg ggtgatcagt tctacagtag cctcgtcacc cttgaggact
     4141 atgctctgaa cattgctgtt ctcgatctgc gcaagcaact gtgtatagct gagttgtacc
     4201 ggattcccgg agtttgtata tatcatcagg agaaacagta caagtacgaa tataactata
     4261 taaaagctta aacctttaaa atacttcaaa aatctccctc ctggaccaac gatggccgag
     4321 tcttctttac agattatatt atattaacat aaactatata taaacacaat taacgctggg
     4381 gtcctaacac atatataccg ccggcgttac atttttgtct gtattcagtg ctttttttca
     4441 taaacatcct gcctgagcgt gcaaacatcc ggcagattcc ggtatttgcc cgcatagtcc
     4501 aggccgtaac ctactatgaa tttatcaggt acttctatac cgttgtactc gacctcgatc
     4561 tccaccttcc tcctggacgg cttgtcgaat gcggtgcaga tcttcacgct tttcgggctc
     4621 ctcgtattga agagatcctt cagatgcctg agcgtcaggc ctgtatcgac aagatcctcg
     4681 acgatcagca catgttttcc ggcgatgtcg aaatcgatat cttttattat ccgcacgacg
     4741 cctgaggatt tcgtcgacgc cccgtagctt gaaaccgcta tgaagtccat gtcgacaggt
     4801 atcgtgattt ccctcatcag atcggccagg aaaataaacc cgcccttcag gactccgaca
     4861 aggacgagct cttccccctc atagtcttcg gatatgcgtt tgcccagccg ctttaccatt
     4921 tcccttatct tctcccgcgg cacaagaacc tcttcaatat ccttcatcat agtcatgacc
     4981 cctccgattg aatgcgattt tcagcacttt tttagtattt tcagttacct tgaatttatc
     5041 acttatttta tatcctatga cccatactat ctcgttcccg cggcatatca gcggtatgcg
     5101 gcctcgtgcc tcgcgcggga tcttgctgtc gatgaaaaat tccttcagtt tttttgtgcc
     5161 gcccgaacca aaaggtctga aaatatcgcc ggcccgacgg tttcttatat gtattccctt
     5221 tttaagcagg tcataatcaa aatattgcac gtatggatta taccccagtg atttatattt
     5281 atcaatactg agcgccgggt ggaccgatgc cgacagctct gcgccggcct ccggcacttc
     5341 cgtcgtcccg gggatcgaca cctccctcga aaattcgccg gggtgccgtt ccttacctgc
     5401 tgcgctttct ccgcaggaca ccttcaggat accgtatgaa acggatgccc gtataccccg
     5461 cggtaattct gccttcatac cggtggcgcc tttcatgacc agctcggtca aagcctgata
     5521 atgtacgcct cctacccctt ttgcattccc tgacgcatgg atgacggcga gcctgaggac
     5581 acgcatcagt atcgccgtat cgagttcccg aagtctgccg atatccatac tgacctgtcc
     5641 gtctcctttt tcgcgcagaa cccgctcata tgcttcagct gcgcaccttt ccatgaaaca
     5701 ccggtcagcg gcggcattct cagccagcct gcaaaggctg ttgacgatat tggcgccgaa
     5761 gttttcattt atatatggaa acaggccgag cctgaccttg tttcgcgtat attcgccggt
     5821 cagattcgtg ctgtctgtcc tgggggaaag tccggcctcc ctgcagtatc gttcgatctc
     5881 atccctgtca gttgaaagca gcggccttat tatattcccc ctgatatatt ccataccgca
     5941 aaggcctctg gtcccggtcc cccttattat attcagcatg accgtttctg cctggtcgtt
     6001 cctgttatgt gccactgcta tcttggatgc gcccacggtc ccggcatagc tgccgaactc
     6061 gcggtaccgg atctccctgc ccgcttcctc aactgaaatg cccatttttg ccgcagccgc
     6121 cgctacatct gtccgcatga catgcagagg tatcccaagt tcactgcaaa ggcgcgcggt
     6181 atactcctca tccgcatccg cctcttcacc gcgcagcatg tggtttatat gtacggcata
     6241 cagtttcatt ccgagttcgg gtcttagaga actgagcaca tggatgagac agacggaatc
     6301 aggcccgcct gaaatgcctg cgacaacacc ctctccgtat tcgatcagtc tattggcgag
     6361 aattatatcc atgatcttct tttggaattg ttgaacgaaa ttactgatag gttccatctt
     6421 tttccaaatt cgcaaacttc gtgtatttat cgaaccattt tagatatacc gtaccggtcg
     6481 atccgttcct gtgcttggcg ataatgactt ctgctatgtt cttcttttcg ctgtccggat
     6541 tgtaataatc gtccctgtaa aggaacatga ctatatcggc atcctgctct atggctccgg
     6601 attccctgag atcgctgagc ataggccggt ggtccgtcct cgattcaggt cccctgctca
     6661 gctgcgacat ggtgacgacc ggcacgttca gttccttagc cagtattttt aacgaccgtg
     6721 atatttccga tacctcctgc tgcctgttct ccgattttcc tcttccgcgc atgagctgca
     6781 ggtagtcgat caccacgagc ccgagcttct tttcgagctt cagcctcctg cactttgccc
     6841 tgatatccat tacagtaagg cctggcgtat catctatata aatgggcgcc tccgacaaag
     6901 ggccgagagc ctttgctatc tttttccagt cttcatcgtc cagcttgcct gtcctcatct
     6961 tgtggctgtc caccatgacc tcgctgcaca gcatcctgtt gaccaactgg tccttcgaca
     7021 tctcaagatt gaacagcgct accggcacat gtttctggac cgccgcgtat tgggcgatat
     7081 tcagtgccat ggcggtctta cccatgcccg gacgggctgc gatcagtatc aggtccgaat
     7141 tctgcagtcc ggcggttttc atgtcaaggt cggtgaaccc tgtcggcact cctgttatgg
     7201 ggcttttgct gttgtaaagc tcttcgagcc tgttgaatgt ctccaggagc acgtccttga
     7261 tatgggtgaa cccctgggtg ctccgtcttt caattatgtc aaatatggtc ttttcagctt
     7321 tgtccagcac gaattcggct tcctcggcgc cttcgtagct catccctgcg atctcctgcg
     7381 acgcttttat caatttgcgc agcagggatt tttcctcgac gatttttgca taatgccttg
     7441 catttgccgt ggtgggcacg gcactggtga tgcttgcaag ataatcgatc ccgcctactt
     7501 tctccagcgt ccccctttgc tgaagctgct cggcaacagt gattatgtcc accggtccgg
     7561 ctttttccac tatatctatt atggcctcgc atatttccct atggtcttcc ctgtaaaagt
     7621 ccgaactcct gatgagttcc gtcactgtcg ggatcacgtc ggaatcgagc agcatacagc
     7681 cgagtactgc ctgttccgcc tcaatattat gaggtggcac cattcccaag gcactaagat
     7741 ccactttacg acccctccgg ttatctgttg tctgtgcttt gctatctgtt tcccgtactg
     7801 atcctggttc cgcttctgac tctcattccg gtttctgttc agatccttac tccgactcta
     7861 ttctgacctt cagcctgccg ctcacttcag gatacagttt cacgtcgagt tcatacgccc
     7921 ctgtcgattt tatcgcatcg ggcattacta tctttttcct gtctatatcc agcctgaaat
     7981 cttctttcag tttatccgaa atgtccttgc tggttatcga tccgaagagt ttgccgtttt
     8041 cgcccgtttt tgccctgatg actacttcca cgttccggag ctcctccgcc agtttccttg
     8101 cctgtgcaag ttcccggtcc ttcttgctct tctcggcctc tttcctggtt ttcactatat
     8161 tcagattgct ttcgttggcc tcaacggcaa gaccccttgg aaataaaaaa ttcctggcat
     8221 aaccgtcgct gacattaacc aggtcgtctt tcttccccag ccctttgaca tcctgcttca
     8281 atatgacttt catatgaacc tccggtttaa gtattcggtt ttgccatctc ttctttataa
     8341 ttcattatag catattttaa tctctctttt gcatctttta tgtcagtgcc gggaagctgc
     8401 gccccggcca ccgacatatg gcctccgccg ccaagcttct cgagtatcat ctgcacattt
     8461 atttcgccaa gggatcttcc gcttatccag acctcgccgc cgacactgca caatacgaac
     8521 gatgcggcga tccctgacag gttcagcagt tcatccgccg ccttggctgc tataagctga
     8581 ggattgtcga tatcttcccc gcagaccgat atcgctatat ctccttctat cagttcggcg
     8641 ccgctgacaa ctgcggagat cttcgaataa gtttcgatgt cattctggaa cagcctcttg
     8701 accgccaccg tatcgactcc ctggcgtctc aggtaggatg cggcttcaaa ggtccttacg
     8761 ccggttttga acgtgaagtt cttggtatcg acgacaatac cggcatagag ggcctcggcc
     8821 tcgatctggt tcagtttcag cttttcatct atgtactgga ccacctctgt gacaagctcg
     8881 gatgtggaag acgcatatgg ttcctggtac gtcaggacgg tgtcctgtat gtaatcggcg
     8941 cctcgcctgt gatggtctat caccactgtc tgttcgcttt cgtctatcag ttccggcact
     9001 tcggcaaaac ttctgcggtg attgtccaca acgatgagga gcgtcttcct ggtcacaaga
     9061 tctattgctt cgtttccggt caggaacaga tccgcatatt cctcgtcttt ccttatcatc
     9121 tccgcaagtt tctcgatggt ggggtttatt ttgttcagta ctatgtacgc atctttgctt
     9181 ctgctcctga cgaccctgta aaggccaagg gaagctccca gcgaatcgat gtcgccgttt
     9241 tcatgcccca tgatcagcac acggtcggcc tggtctataa gttcgcgcaa tgcatacgct
     9301 atgaccctcg cctttacctt cgtcctcttc tcaagttccc tggactttcc gccgaagaag
     9361 ctgaagttct cgccgttttt cagcaccacc tggtcgcccc ccctgcccag cgccaggtcg
     9421 atggaggcag cagcaaaccc aaggttttcc gccagcgtcc cgccgttgag accgaaaccg
     9481 aggctcagtg tgacaggcag tttattgcct atgtttatct ccttgacgga atccagtatt
     9541 tcaaatttct tctcttcaag gtccttcata tacttctgct caaaaatgaa caggtatctg
     9601 tccctctcgt acttttttat gatcccgttg gtaaacgaga tccatttgac cacccgtttg
     9661 tctatttcgg ccagtatctg cggcttgagc ggatcttcca tgctctgcat caggtcttcg
     9721 taattgtcta tgacgcatat gcctgcgacg atcctctcgt cggcataagt ctgcttaaga
     9781 ttgacaagct cggtgttatc gatgaaataa aggatcagga tacagttcgt tatatcctgc
     9841 tgatcccgtc tcgctatact gatgagcaca ttataatggc ggccgttgat atttatctgc
     9901 ctcgatatcg gcatatattt ccggtctgaa ttatcgacag gcagttcgct gcggagttct
     9961 ttcacaactg atttgaccgc gttctcgaat atttccgtat gctcggaaat atccctgaag
    10021 gaggaattat accagatgat cgatccgtca ggctccgaaa ttaccagcgg catggggaag
    10081 ttgagcagag tatctttcgt agccgtgtcg atgttgaggt tcaggttttc gatgaactcg
    10141 gatatttcgg cttttcgcct gtagtttgtc ctgatattgt gccatgcaag gtaaacaaac
    10201 agtatgaagc acggtattgc caccatccag tcaagcactg ctatgaccac ggtcaacatc
    10261 agtatgatcc atatatggat gcttacctgc ggtatcagtg tgctcaggat ttttttgctg
    10321 tccatatccg cgaaagggtc tcccctattt ctcctttctt ctgctgctgc ccagatgctt
    10381 tgaaagctcg gacaggtcgc cgtaatactt ttctacatca ttgttttcca caagtccaag
    10441 tctgaccttg ctgcgtatct tcatttcaac tgcattgtag ctgacgccga gccgcttgcc
    10501 cagcaggtag cttataagga tgacgttcga cagcacatcg ccgacagcct catgaagctc
    10561 ttcccgggcg ccttctgcca gcaccttgaa aaggtccgcc acttctgtga gcatttcgct
    10621 tttcaggcat tctatgatct taatgttccg tgttatatcg atctcttttt caggcaatgc
    10681 catatactca actcccaacc cggtttgcta atattttacc atattatggt atttcctgca
    10741 tcagatttta tcaacaggtg aaaccgggta ggagcccaat atttttaaaa atgaagtttt
    10801 tcttttcacc attgtcaggg cgtccctaag gtcctcgtcc tccctgtgtc ccaggacgtc
    10861 cacgaaaaat atgtatcttc cgagcccgct cctggcaggt ctcgattcga tccgcgtcat
    10921 gttgatgtcc cagaggttga aaatctgcaa aatccggtaa aggctcccgg gtttgtcttc
    10981 agtcgaaaaa atgagagaag tcttgtcgtt cccggtcttt tccgcatcgc ttcctccaag
    11041 gacgataaac ctcgtaaaat tgctgtcatg gtcctggatg tcttccctca ggatctccag
    11101 tccatacacc tcggccgccg ccctggatcc tattgcagcc atgccgcctg tgcccgccgc
    11161 cacttcttca gcggctgcgg ccgtgctgtg ggcggctttc aaaaccgcac ccggcaaatt
    11221 ttccgccaaa aaccgcctgc actggccaag gggctgagga tgcgaaatta ccgtcctgat
    11281 ttctgaaata ccggtccctt ttttcaccag caggtgctct cttacgggaa tgacaacctc
    11341 agccctgatc cacaggccgt tgtcctgtgt catcatatcc atggtcgcat tgaccgcgcc
    11401 ctcgatggag ttctctatcg gcacgatagc ttcatccagc tgcccgtcca gcattgcagc
    11461 aaatacatcc ggtatcgacg ggtattcccg ggatatgtat ggcacctctc ctgtgtactt
    11521 taccatggcc tcatgggaaa atgtccccct ggggccaaga tatccgattc ttttcatgcg
    11581 atgacatact ccttaaaaat ccggctggtt tggcgtatat aataatacaa caaaaactcc
    11641 gtttttccaa ctgcattatc aaaacgaccg gatcgccagg ttatgcatcc ggtcgtttgt
    11701 tatgcttgca aaaaacccct cctgacccat aacggatccg aaggggccct gatccgacat
    11761 atatcagtcg gctgtgaacg gcagcagcgc gatggtcctt gctctcttta tcgcggtcgt
    11821 cagctggcgc tgatgtttcg cgcaattgcc ggagatcctt ctcggaagga tcttgcctct
    11881 ttcggaaatg tatttcctca atttcgcgac atctttataa tcaatttcgg taaccttgtc
    11941 aacacagaaa gcacaaactt tcttcctgga tcttctcttt atgttgactt tgccttcgcc
    12001 tttgtcgtaa tccttgttag cgctcttgtt atccttttta gctgtcatgc caaacgcctc
    12061 ctttccacta ctgttgcagg gtttacggcc ctgcgccggt atccggcggt aaggtcactt
    12121 atcagaacgg caggtcatca tcgtctgtcg ggaagaatcc ggcatcgcca tccgaccctt
    12181 ccgctgaagc aggtacgaat cctgaagcgc ctgtcgggcc gtatgcggat cctccttcgg
    12241 ttctgcggga atcggcaaag tacacctcat cagcgactat ctcagtgaca tagtgctttt
    12301 tgccttcgtt gtcgtcccag ctcctgttct gcaggcttcc gacaacaacc accctctgcc
    12361 ctttggcaaa atatttgctg caaaactccg ccgtactgcg ccaggcgact atgttaaaga
    12421 agtccgtctg cttctcttcg cccggtttgg aaaaccttct gtccaccgca attgcgaacg
    12481 agcatctggg tatgttgctc gaagcagtat atctcagttc gggatccttt gtaagcctgc
    12541 ccattaatat gaccttattc atacaaccac cctatttttt cttaatgatc atatatttga
    12601 gtactccatc cgtgatcttg agtatccttt caagctcacg cgggaaatcg gaattcgcgc
    12661 tgaaatcggc aagcacgtaa tatccctcgg tcttgtccgc gatttcatag gctagctttc
    12721 tttttcccca ctcatctatg ccttccagtt gtgctgaggt ttcgagcaga ttcttgaatt
    12781 tctcgacaag agcttttgtg ttttcttcat caagatcagg atcgatgatg aatatgcact
    12841 catatttgtt cagcatgtga ctttcacctc cttctggact aaacggccct gcatcggtgt
    12901 gcagagcaag gattctgagt aattgtatca caatggataa gcatgtgcaa gcaaaatgta
    12961 tataaactta accggccatg aaaccggccg gcaaagcaca tacatataac ctgtcaacaa
    13021 catgattcta catcattttg aagatgatcg caagcaacag cagaacagcg gccgccagca
    13081 agatgtttac aacagaatcg accgacatcc ccgacctgaa cagcctgccg ggcggcagcc
    13141 ttttttcacg ttttttcgta aggccgtgct cctttatgta ttcatttatg atgtgctccg
    13201 cttccctgag cagaaaatcg tccttcggtt ccggcctgca ctgcccgtct ttctccccgc
    13261 cggcgtcagc aggttcgtcc ggccctcccc tgaggatcag tatggcctct tcgatcagat
    13321 tcgaggcaat gttttttatg actactattc ttcttgaacc gccagccacc atatccatcc
    13381 tccaaccata tcctgtatcg tctgccggaa acaagccgta cggcatatcc aacaggtata
    13441 gtttgcgaaa aaaacaagga tttattcaat attttcctgc gtatccgtcc gcaggacgac
    13501 gtgctgtcac aacggttttt tccatacctt tgccacaagc accgtcatgt catcatacgg
    13561 cttgccgtca cagcgcctga cagcctcatc gaggatcgac tcggctacaa gctgcggatt
    13621 caggctctcc aactgccgga tatgtttcag cagggacctg tctcccggtt catcaccagc
    13681 cattgcgtct gtcaccccat ccgtgaccat gatgatcata tctcctgcat ccattatttt
    13741 gcgtgaaagt tccgcatcga cgcctggaag tatgcctgcc ggcagtgacg ccgacctgat
    13801 cacttcgacc cttgaatccc tttttataaa tgtgggcgcc gcgcctatct tgataaattc
    13861 cacttccccg ctggtcaggt ctatcatggc gatgtccatg gtgcatgtgc tgtcttcgtc
    13921 agatttcaat accagtacag agtttatcag gcttactgcc atgtctttgt caaacccgga
    13981 ttcaaggaga ctctcaagca tatccactgc agccttgctc tgcgcagacg cactgtgtcc
    14041 cgtacccata ccgtcgctga gcgccagcgt gtatttcccg ctgatccctt ccatgaacga
    14101 aaagctgtcg cccgaaaccc tgctcccatg ctttggcatg cgtgccaccc ctgttgttat
    14161 cctgaggttt tctgcttcca tgtatttcag gatacactct tcgctgccgt acgatgtaca
    14221 gccttcgtcg ctgcgcacca tccttctgcc tgtggtttcc gagacggccc tgtctattgc
    14281 cgccgtgcat ttccttgcgc cgccgcacga ggaatggcgc acatttatct cgtattttcc
    14341 ccatagccct ttgtatgcta ccacctcaac gaccctgacg ccttccttct tcaatgctgt
    14401 tgaaatggca tcctccagcg ggctcataaa gcttatatcg gtatttatct cttcggcaag
    14461 gttgcctatc acacgggaca ttccttcgaa ttgcctgctg atcacggacc tgctctcgcc
    14521 gatcttgctc ttccatacga ctcctacttt gaacagctca tacatgttgt tgaccgcatt
    14581 gacaaaatcg tttatcctgg gacacttgtc tataaaatcg gcgggtatgt cgctttcctc
    14641 cacgcgtccc ctggcttcga ggctttcgac gatccggaac attacctggt acgtgtcata
    14701 aaagctgcgt tcccagcagt gcaggcaaag gctgcagtca cggcatatcc tgtccgccac
    14761 cctgtcgaac agtacgttta tatcatgttt ccctgcagca gcgctgttcc gggccatctc
    14821 tccgaaactc ctggacaaag ccatgaatgc atctgcaaat ttcctgagcc tttgggccgt
    14881 caggtctctg atcctgcgtg aataactctt catgtccgca tgttctgcgg attttctcgc
    14941 aaagggagcg gtgaagcggt ccataagcct gtccggcatg atgaaaaaaa caacggaagc
    15001 agccagtata tccctgagat atagcattga ttccgcaggc ccgttgaaat atattgcaag
    15061 gatcatgttg cccagtacaa agccaagcgc cgatccggct ttcccaagac tgccaagtat
    15121 gcctgccagc atcccgcaca gtgcatatgc gcctgccgta gacggggtga accccttaga
    15181 agcgctgacg atgagacctg ccgctgcccc tgtggcggcg ccggctcccg cactgcattt
    15241 gaaactgtag aaaagtacaa gaagcacgca caggatattt ttcagggaga aaccgaacaa
    15301 ctggagagcc ccgaaaccgg aaacagccaa tgcggcagtg atgcctgcgc tgactgcctc
    15361 ctcgccgtcg agcgctgctt tcccggccat tcccgaaatg acaggtatgg agaacctgaa
    15421 tataaagtag aatacaaagg atataaatga acagaacagg gatttcagga catcatacag
    15481 cagaaacccc caaatgcccg atagtatgag ctgcggtaca aggatcgcga tgaatgccgc
    15541 aaaagcggcc caaatatcca tttttgaatc ggttttcttc agcgggatcg ataagacaaa
    15601 aaacagcagc atgcacgcag cattgatgta tacgagttcg aacgggccca ggataaaagc
    15661 tcccaacatt acagccgcgg ctgtcatgag cctcgggact cctgctccgc atgaagcggc
    15721 aaagaatacc gctcccagtg ggaccgcagc agatatgctg ctgcctgtta tcaggaatgc
    15781 tgtaaggaat aaaaggatcc tgttcctctg aaaatataac ctgttgataa gcttcggcag
    15841 atagccgggt ttgctcattt gtacagcggt gatcttcatc tcttgtccct cccgtaccac
    15901 agattataac ggagggagac aggtttttat gtcattttgc gctgtaccgc aataaaaagt
    15961 ttatgacacg gtccagcaaa aacccggaaa tttttcagga ccgggcaata aacttatctt
    16021 ttcttcctgt tgtcttttgg tccgatgttc aatccgggtg atttatcgtt tttgccgttc
    16081 ttatcattct tatcgttttt cctcgtatcg tttttttcgt tttcattttt gctgtcacgc
    16141 tcatcctttt tgctgctgtc cctgatgtcc tgctttttgt tgtctgctgc gtctttcccg
    16201 gcatcaggcc gttttccccg gggagtatct atggtattgc ctatctttgc tttgtctttg
    16261 cttatctgat caaggaactc attcctgagc cgttctcttt cttcttgcag tttctctccc
    16321 tgcttctcca gggttttccg gacatcgtcc caactgagca ggtccggttt tgcatccttt
    16381 atccctttat tgctgttttt gctgtccagt ttgccgttgc cttcacccgg ctgttttata
    16441 tcgttctgcc gcgggttgcc tatgcccggc aggccgctga agccatgatt gcctcccccg
    16501 ctctgtactc cggcattctt gtccttcctg ctgtcatcga tgtctttttt gccggtgacc
    16561 ttgcctgcac ctgcaccggg tttgatgccc ccgccgcctt tgtcttcctt cgggccgctg
    16621 ttctggccgc cggtattgct gccgccgggc cagggccaaa tggtaccggc attgctgcca
    16681 ttagtgctga tgccaatacc ggtgccggta gcggcactgc tgttcttatt atctttattg
    16741 tctttgatta ctttattgtc tttattgctg ttgcttccgc cagcagcggc attggctccg
    16801 ggttttcctt ctttcgcctt gtcttccttc ttttctttgt cttctttctt cccagcggat
    16861 cccttcgcaa gctgttcttt cttttccctg accatttcca gcaggtcttt taccgcagta
    16921 ttcttcattt tctcaaaatc cgccaggggg aaataaccgg acacttcttc gatcagtgcc
    16981 agcttgcccg gtgttacacc gagcttgtgg gcggcttccc tctgctttac gctggtctct
    17041 cccacgagta tcttcgattc gacattgtcc ttgttgagtt ccctggccgc catatcttcc
    17101 acgatcttct taagctcgtc agcttttttt gcattgctgc tcgagacggt aagtatcacc
    17161 gcattctgca gtttgccgct gccgccggaa ggtttctcct tctgtttccc ggtatctcct
    17221 tctgtctttg cggacggttt tgcggtacct ttcggatcac ttttgccgct cccggcattt
    17281 cccccgcttc cctcggccgc gctgccgctg tatcctgcat cactcccgct gtcggcagtt
    17341 tccgctccat tttccggcgt ctgcccgccg ccaggcgtca gggtatgatc ggaggtatcc
    17401 tgtggcgcgg aagttccgtc ctgtacatca gccgcatcct gccggcctgt gccgccggct
    17461 tcacttttac agcggctgcc cgtgttctct ttgagatatc cctgttcgac agcgctgctt
    17521 agcagcagcg tcactccttc gtccagtctc ctgtgcatca ggttcttgtt ctcgatgagc
    17581 ttgctgccgt catcgttaag cgcttcaacc ctgattatcc tgtcgtatat gttggctgtt
    17641 agcccaatac tggggtttat gtcgaaatcc acatagctgt agggcatact atagctgaaa
    17701 atgccgattc ccaatgcgag caggaaggac gctgcgatgg aggcgatcct gtacacgggc
    17761 tttctggccg ctgaaggcaa ggaaggcatg ccggtatctg tttcagcgcc ggcagcaata
    17821 ccgggctgcg cctttatttt cctgaactgc ccgtcctgtg tgagtattac cgcatacttg
    17881 cccgttatct ccataacgat gcctttcatc tgcatccctc cttcctacag ctttatgtaa
    17941 tccttgatat attgataatc ccctattgca ataacaacta tggctataat atactttcgg
    18001 aacctttcga tgaattttcg gggtaatccc gatgctttct ccagttccat gacaggaagg
    18061 tatttcttcg tcatcaccga ttccagcatg tcgggccgtg acaatatgat ccctgcgagc
    18121 ccggcacact gctctctcgt tctcctgtgc ctgggcgaaa cttttgccag gtcccggtaa
    18181 gtgatattcc attggctgag ttccttcgcg agttcctgta tctccgcctt cctcagttcg
    18241 gcaatgtccc ggtccgaaaa ttcccgtatc gcttcatcgg cgccgaggtc atattccgtc
    18301 tccttttctt cggcaaaaac gttaagaggc accacnnnnn nnnnncgtct ctctttcctg
    18361 tagtaatcga taagcctgcg tttgatcacg ttcctggaga aagtgaagaa attccctttc
    18421 gaactgtcaa aggacctgat ggcttcaacg aatgcgatca tggcgatgct tagttcatcg
    18481 tcggtatcat agctcatata cctgccggtc actttttcgg cgcatgacgc tataaaaggt
    18541 ttgtatacct ctacaaacct tcccgtctct tccgcgtcgt cgcgtatgct cgatattctc
    18601 gcatttattg cgtcgaattg catatgtatc ccgtcctgtc ccgcctgtcg ggccatgcat
    18661 gctcccatgc ggcggcagcg gaagacagac cttcgggtcc gtcttaagtt ccgggaaaat
    18721 tatagcataa agattccggt tgatgtgctc atgtcacaat atagtaattt tattgtaata
    18781 aaatttaaaa caaaataaaa aacagcagac attctccttc tgccgttttt tattgctgta
    18841 tcgttcgcca tctgttttac tgttccatct gcctcccaag gaaacctgcc gcagactgcg
    18901 caagctttgc ttcgacttct cccatcctgc ttcccggatc cgtcgacaat attataaccg
    18961 cccctatcgg atctccctca gcgatgatgg gtgaaacgac ctgcgccaca tacttccttt
    19021 cattgccttc gtcggcgagt atggatatac tcttgtcttc cgctgatttt gccacgaaag
    19081 tcgtcttttc ctcaatgacc ttttccagtt cagggctcag cgacttgtca aggaattcct
    19141 ttttggatgc gcccgccact gctatgatcg tatccctgtc ggcaatgcat gttatatgcc
    19201 cgctggtctt gtgtatcgat tccgcatatt gagcggcgaa atcacccaat tccccaatag
    19261 gagaatattt tttgagtatg acttctcctt ctttatcggt gaatatctcc agaggatcgc
    19321 cttctcttat ccttaaagta cgccgaatct ccttgggtat cactaccctg ccaagatcgt
    19381 caattctccg cacgataccg gttgccttca atgtcgttac ctccttaaca gaattgttta
    19441 acattgcttt tttagtattc attgtttatt ggagttttat gcataaaaac aacgggccgc
    19501 catttcaggc agcccgcgga aaatcgacga cgatcggata ttttccgatc ctggtccatt
    19561 tatttgatcg aatcgtaaac ggaattttcg atcttatact cggctttatc caccaattct
    19621 tttatccttt gctcatatat gaatgccgca ccgtattcct ggtactcctt cgcgcacctc
    19681 aggcttacag gctcgccctc ggcgtatttt tcttcaagtt tgattatatg atatccatac
    19741 tctgtcttca caggttcctc cgtaaactcg cccggttcca gttcaaacgc ggctttttcg
    19801 aattcatcga ccatcctgtc tttcccgaaa agatagtcgc cgccccattg tgcagaaccg
    19861 tcatcggaat tctctttcgc cagggtcgcg aaatcttctc cggccttcag cttctcgatc
    19921 agtcctttcg ctttttccag cgctgcatcc cagtcctcgt cggaagcatc tttatccacg
    19981 gagatcagta tatgcttcgc ccatacagct tcttcagcat catatctgta ttttgtgtca
    20041 gctttgtaca ggtccgtatt ctccgcataa tatttttcaa tatccgcgtc tgttattttc
    20101 cctgtctctt ccgtctggta tttctggact atgaaaatct gctcctgtat cttttcatac
    20161 tcgtcaatgc tgatcccgta attttccttg aatgccttgt tggctttgat cctgttgccg
    20221 ttgccgtacg gctcgattat ctgtttttct gtcagtaaat cgatataatc cttttcatcc
    20281 ctggtcagtt ccacattcgc ttctttcgcc atcctgtact gtactttcaa acttttcagt
    20341 tcctcgagga tctgcttctt cgctacttcg accggatctt cgccgccaat tttcgtagcc
    20401 cagaacgtct cttccgttat attcggatct gcctgctgtg cgctgtaaaa catcaattgt
    20461 ttctggtaat cgagataata ccggaactca gtttccgtga tcttctctcc gcctaccgtc
    20521 gcaactatcc tctcggccgt attgaaatat atgatcagac cgattgcaat ggcaaggacg
    20581 attgcaccgg cgatcaggcc atatttgaca aacaaaggca ttcccgtttt ctctccggcg
    20641 taatcgccgc cggagctctt tttcgttttt ttatcttcca aagttatctc tcccctgata
    20701 tattatgaaa acgatatttc tgtttacaaa agactgtgct gatgtcaatt tacattataa
    20761 tagataatca ccctgtatca tagcttttta agtcctgtaa caaaatttta atattgtcgg
    20821 ccaggctgtc cgcgctgatc tgcggtttcc tgtatacaag gtaaggagat gctcccgcgc
    20881 tgaaaagcag ttgccttttg tacttctcgg caatttttgc gatcgcctgg aagttgatgc
    20941 ttttgctgtc cgcaaatgca aagatgacag aatccttctt ctccgtcacg gacgaaatgc
    21001 ccagggacct ggcaagcgcc ttgatatggg cgaccttgac caggttcctc acgggcacgg
    21061 gtatttcgcc gtacctgtcg gtcagttcgt cttcgatgtc gatcacgtca ttctcatcgc
    21121 ttattgccgc gatcttcttg tacatctcta ttttcctgct ttcgttccct atatatgaat
    21181 catcgatata cgcatcgatg tttatatcga tagtaacttc agcctcttcc ttcctgggcg
    21241 gttcgcccct gagttccctc actgcctcgt caagaagccg gcagtacatg tcataaccga
    21301 cggactccat gtgtccgtgc tgctccggcc cgagcaggtt gccggcgccg cggatctcca
    21361 tgtcccgcat cgcgatcctg aagcctgatc ccagttcagt aaattctttg atcgcctgca
    21421 gcctcttttc ggcgacttcc gaaatgatct tgtctttcct gtatgtgata tatgcatagg
    21481 cgagcctgtt ggagcggcct accctgcccc tgagctgata aagctgggca agccccatct
    21541 tgtcggcgtc ctcgacgatg atcgtgttca cattgggcat gtccaggcct gattcgatta
    21601 ttgtcgtaca aaccaggata tcgaattcgc cttttatgaa gctcatcatt atgtcctcaa
    21661 gatccctctc gtccatctgt ccatgtgcga cagccaccct cgcatccggc accatgtcct
    21721 ggacttcggc agccttggta tctatcgtcc tgaccctgtt gtacaggtag aatacctggc
    21781 ccttcctcgc cagttccctt atgatagcat ccctgatgac atcccggttg tactccatca
    21841 cataggtctg gaccgggtac cgttcttccg gcggatcttc gatgacgctg atgtccctta
    21901 tccccgtaag cgacatgtgc agagtcctcg ggatcggtgt cgcagtgagg gtcagcacat
    21961 cgacctcggg ggcaaggttt ttcaggcgct ccttgtgcga gacgccaaac ctctgctcct
    22021 catcgatgac cagcagaccc aggtccttaa accggatatc cttctggagg aggcggtgtg
    22081 tgccgataag cacgtccacc ttgccgtttt tcacatccct gagtatccgt ttctgctccg
    22141 cccgtgtcct gaacctgctc agcatttcaa cagtcacggg gaagtccttc atcctctccg
    22201 caaagttatt gtaatgctgc tgagcaagta cggtcgtcgg gaccaggtag gccacctgcc
    22261 tgccgtccat gacagctttg aaaacagccc ggatcgccac ttcggtcttg ccgaacccca
    22321 catcgccgca aagcagcctg tccattacct ttccggattc catatcctgt tttatctcgt
    22381 cgatgcacct taactgatcc tcagtttcct catacgggaa ctgctcttcg aactgtctct
    22441 gccatactgt atctttcgag tattcaaagc ccttcagtgc cgctcgccgg gcataaagct
    22501 ttatcagttc ttcggcaagc ttcttcagcg attccttgac cctggtcttg gtcttcagcc
    22561 agtccgatcc tccgagcttg ctgatttttg gctgtttccc ttccgtccct atgtatttct
    22621 ggatcaggtc catctggttc gtgggtatgt aaaggtatgc tccgtcctgg tacctgatct
    22681 tgagataatc ctttttgatt ccctcgacgg tcagcttttc caggccgaca tactggccta
    22741 tcccatgggt ctggtgcacg acataatcgc ccaccgccag ttcggtaaac aggctgatgg
    22801 gacgcccctt gtatttgccc tggctccttg cggttttttt ccggtcacgg ccaaatatct
    22861 catcatcgct tataacgacg aatcctatgg tcggatattc aaagcccctg tggaggctgc
    22921 cgcgggtgat gacgacctgt ccgggcatga cgggccttgt gacgtcgtcc atatagacgg
    22981 attcgatccc tttttccgaa agcgaatccc tgagccgttc cccgcgcccc ctggtgcctg
    23041 aaagaagcag caccctgttt ttgctgcgct tccacatgga aaggttttcc gcaagtatat
    23101 ccatgctgcc ctggaacgat ccgatcatcc tgcatgggat gctgtatgaa atggatccct
    23161 tcgatgctat gctgtctgcg ttgacagtgt tcatatatat gggcttcctg gccgatacgg
    23221 ccgagaaaat cgattcgtaa tcaaaataca tgccggtgct ctgaggcagc aggcttcctt
    23281 tttcaatcag agccttgcat atctcctcat gctcgagcag caggttttcg agacgctgct
    23341 cctgtcttgc cggctcatcc atgaaaataa gggtgtcatc tccaaggtag tccgccagtg
    23401 aacatggttc atcgataata tatggaatat atctgtcgat gcctggaaag taccatgatt
    23461 cctcgagccg ttccatatcc gcgtttattt ttcggaccag gtccggatcc ctcttccccg
    23521 tcttttcagc gtcgctcttt acggatctga tgatctcgga cctcctggca atgtcgaata
    23581 tcagctcacg cgcaggaagg atcctgacgc tttccgcatg atcaatggac ctctgcgtat
    23641 ttacgtcaaa ccatctaagc gagtcgatct cgtcgccaaa aaactcgatc ctgacaggat
    23701 tatcggcatt caccgggaaa atatcgacga tgccgcccct gaccgcgaac tggccttttt
    23761 tctcgacgaa ttccaccctc tcgtacgcgg agaccgccag agtgcggata agctcctcag
    23821 ggtcaaggcg cccgtgatca ccgacagtga cgacattttg caccagtttc cccggatgga
    23881 gcagcttgtg ggaaacggct tcaacggaag tgacaacgaa gctgtaatca ccgcggctta
    23941 tcctgtcgag cgcggcaagt ctctggaata ccgtgtcgta gctttttgcc tcgacatcgt
    24001 aaagcatgat ctcctttgtg ggaaagaata tgacgtcatc gccgataaag aaggagaaat
    24061 cctcgaacag ccttctggcc tgcatttcat tgaacgcgac aaaaacgccc ttcctgccgc
    24121 agtggcggca aagggcatac gaaatatgag ccttctgcga ttcagaaggc ccaatgatgc
    24181 tgaccggatg cttactctcc cggacagcag cc
//
LOCUS       DAOSCC010000009        22817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1628_length_22817_cov_4.548150, whole genome shotgun sequence.
ACCESSION   DAOSCC010000009 DAOSCC010000000
VERSION     DAOSCC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22817
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_22817_cov_4.548150"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1020
                     /locus_tag="PLG72_01215"
     CDS             61..1020
                     /locus_tag="PLG72_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ79455.1"
                     /translation="MSAVVEKVSQPVQGKTATFKSRFENLKKQVIKHKVSYLFVAPFG
                     IIFLVFTIIPVVVSMGLSFTYFNILETPKFISWQNYIRLFLGDDVFLKAVKNTFLLAA
                     ITGPVGYLASLMFAWFINELPPKIRAFMVLIFYAPSISGNVYMIWQVMFSSDAYGYVN
                     STLIKLGIITEPILWLQDPRYVMPVILIVVLWMSLGTGFLSFVAGLQSIDRAQYEAGI
                     VDGVKNRWQELWYITLPNMKPMLMFGAVMSITSSFAVSDVTIALAGMPSVDYAAHTVV
                     NHLIDYGTIRFEMGYASAIATVLFLTMIICNKVVQNLLKKVGR"
     gene            1022..1885
                     /locus_tag="PLG72_01220"
     CDS             1022..1885
                     /locus_tag="PLG72_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ79456.1"
                     /translation="MKHRKLNTKRLNRSLGGDIFSIVILVIFGSFMVLPMIYAICNAF
                     KPLDELFMFPPRFFVRNPTLDNFRDLFLLMAQSWVPFSRYIFNTLFITAAGTAGHVVV
                     ASMAAYALEKHKFPGSKLFFSIVVTSLMFSSVVTAIPNYLVMAKLHWLDTYLALIVPA
                     FGYPLGLFLMKQFMETVPDSLLESARMDGASEFRTFWTVAMPCVKPAWLTLVIFSFQT
                     LWGNTGSTFIYSEELKTLPYALNQILLGGFARAGVGAAVVLLMMIVPVVLFMVTQSSI
                     IQTMATSGIKE"
     gene            1902..3410
                     /locus_tag="PLG72_01225"
     CDS             1902..3410
                     /locus_tag="PLG72_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolactonase"
                     /protein_id="HOJ79457.1"
                     /translation="MRLRKILTAVIIFILLCPATAYADEPYNNYNYDFWGTPVPAPAA
                     YLPSRIIDGRSLGTYEFRKPQDLFTGPDNKLYVADTGNNRIVVINEYWELDRIIDGFD
                     NNGSQDTFNNPQGLFVTNDGEIYVADTSNSRIVHLDADGNLIRIIGRPVSDIIPADLQ
                     YYPTALVVDKAGRIYIIAQGVNQGLIELDNQGEFVGYVGATKVKFNMVDYFWKLIATD
                     EQRERMQLFVPTEYNNIDIDTEGFLYVTTSSLALEDIDRAINTRSKDDRYAPIRKLNP
                     TGTDVLRRNGYFPPVGDVYFRSRGYAESGNSQLYDVCVEDSGIYSVLDKKKGRIFTYD
                     TDGNLLYLFGGIGNRLGNFRDPAALDTINDQFVVLDSNMGQVTVFEPTLYGQLVKEAV
                     VLHLNGRYDESAAKWEQVLKYNANSELAYIGMGKAYLRKDEFRLAMKYFKLGNKRDYY
                     SKAFDLYRQEVIGDNFGWIVISILVLLVGSKILKKVRARKKAGIKAMGKVEA"
     gene            3411..4034
                     /locus_tag="PLG72_01230"
     CDS             3411..4034
                     /locus_tag="PLG72_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ79458.1"
                     /translation="MEVLQSLRYSLYLIFHPFDGFWDLKHEKKGNLKTALLIVAMVIV
                     TFILRRQLTGFILNENKLRELNIIVEILSVALPFFLWCLANWCLTTLMDGEGSFKDIV
                     ITSAYALVPLVLINIPLIVFSNVITLEEAAFYTFFDILSLAWTVLLLFLGTSVVHQYS
                     LLKTFLTSICIVIGMGLIIFIALLFFSLLQKMYSFVYTIYKEIALRL"
     gene            4047..6593
                     /locus_tag="PLG72_01235"
     CDS             4047..6593
                     /locus_tag="PLG72_01235"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOJ79459.1"
                     /translation="MSMKKITALLIATALLMSCSAVFADPGVGQAGGETLQSMKMVAE
                     NDYLALYINEETTEVAVKDKKTGVVWYTNPPDRSEDMIASAINKDKLSAQISISYFTP
                     TSQQKLMNNYTDCIKYGQFEITAIENGVRIVYRIGREEKIYILPQVISKERMEEKILK
                     NLDNATGKKLVNRYRLISLENAKSEKQRQEMLEEFPTVANGDLYVLGDNIKDFMKVQL
                     EEIVISAGYTLEDMNEDHIENNVPPAEENKEHFVIPLEYTLDGDNLVVRVPTDEIEYN
                     ADSYPIYTMSLLEFFGAAGTDERGYILVPDGSGSLIYLNNGKLNAQTYAIDVYGTDQS
                     IPLAVKTSIIEQAYLPVFGMKRGDNAFFSIIESGDAVASVWADISGRLNSYNYVYSAF
                     TLVQNDVLDIGDYSGNNTIMVYQPRVFKGDVKVRYKFLKGEDADYAGMAAYYRSYLEK
                     QGMTREKPRDNVPFFLEVVGAVDKVKSILGLPIKTAEVLTTYEQAIEIINALNDAGIS
                     EIILKYTGWANGGIDHSIPKSLKLMSKLGGSKGFKKLQSFLNEKGIEYLPELGFILNY
                     RDKLFDSFIPMIHASRYITKEIASIYKYNLATNVQQRSLGTFYIISPTKIPSIVDGMI
                     KSLKKLDVTGISLRDGAKNLNSDYKESRLVDRQQARVIVQQQIEKIHESGLSVMAEGG
                     NAYALPYSDYILDIPEESNQFHLTDESIPFFQMVVRGYITYAGTPINLSSDYRKTFLK
                     SIETGSGIYFCFIYEENSLVKESYFDSYYSTEYRLWMNEAKEYYKKANEALSDVQGQT
                     IKNHEKLAEGIYKTTFEKGKEIVVNYTPRRVEIDGTTVEPLDFKVVKEGQ"
     gene            6608..7549
                     /locus_tag="PLG72_01240"
     CDS             6608..7549
                     /locus_tag="PLG72_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ79460.1"
                     /translation="MTNRALKSAAAPQKKKRRGLDLTRKRAAMGYLFVLPFIIGLITF
                     FLVPLVQSFIFSLNKLEVAEGGYNLIPMGFENYNRALRVHASYVRDLWESVRYMIANV
                     PLIIIFSFFAANLLNQKFKGRSLARAIFFLPVIVTSGVMLVVDSGDLLQSSLGMTQTD
                     GTEGAFRAVELARLLLQTRLSERFIDYIIGAVDAIYDIVSASGVQILVFLAGLQSISP
                     SLFEASNIEGATGWENFWKITFPMVSPLILVNTVYTIIDLLINSSNTVMELIRDTAFA
                     SQDYGMSSAMAWIYFAAILLIVGLVMWIVSKLVFYYD"
     gene            7637..8572
                     /locus_tag="PLG72_01245"
     CDS             7637..8572
                     /locus_tag="PLG72_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ79461.1"
                     /translation="MMYTWGKRGWSIVRGVIIAGICFLILYPTLVKLSVSFMPEKDIY
                     DVTVRYVPKSPTLENYKTVIKAMKYGTAFWNTLKLALLTSLMQLISCTLIGYGFARFK
                     FKGRNFLFALVILTMVVPPQTLMIPLFLHFRYFDIFGIISAVTGGKGINLLESYWPFI
                     LMSMTGMGLKNGLYIYIMRQFFRNMPKELEEAAYVDGAGMLRTFAQIMLPSAVPSMVT
                     VFLFSFVWQWTDTFYSTLFLQKTRVLASALSSVSFDVTQKIYFETGVNVALAPAISSM
                     YTNTASLLVVLPLLILYLFAQKRFVESVERSGIVG"
     gene            8795..10231
                     /locus_tag="PLG72_01250"
     CDS             8795..10231
                     /locus_tag="PLG72_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ79462.1"
                     /translation="MSRGLKKLLALSLVTVMMVAVIAGCGQKTGTTPTDTTPDEVEPA
                     PAEQTPSEPADEEPEPEPEEIRDLGGRVIKVAAWWDLTPQGGTPSGDRQVARREEMEQ
                     KYNFKFEYVNVPWDQTVETYSSSVMAGDPFADIATLEDNWVIGLANSGFVTELDSLFD
                     FTDEKWDQVTKSLSTINGKTYGMGTGKWWPRGIVFYNKNIFEREGLENPYELMKNGQW
                     TWEKLEEIARKATKDTNNDGKIDQWGLGGMDIDCSLVISNGSEFASIVDGKAKLNFRE
                     PAVLEAFNFYQRLCKEGIIFNKFNYEAEEIPWDLAANVFKEGKIAMFWYQYWKIDDFR
                     DNMADDYGLVLPPIGPSNTEKKYYCMISGHNFQTIPKNVKNVEDIAFIWNKWTEPFPE
                     DLEDPDAWMEPHMNNVRDQESLEVLKYMYDNNCGKHVGLFGAVRPAVELWWGGQDDLL
                     KGEKTVAQIIDEKYDAIQAALDDFLANK"
     misc_feature    10367..10500
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            10605..11099
                     /gene="infC"
                     /locus_tag="PLG72_01255"
     CDS             10605..11099
                     /gene="infC"
                     /locus_tag="PLG72_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOJ79463.1"
                     /translation="MINEEIRDKEIRLIDSDGTMLGIMSAKEALKIATEKDLDLVKIA
                     PQAVPPVCKLMDYGKYMFELAKKEKEARKNQKVVTMKEVWVKPNIEDHDFAFKSRNAA
                     KFLKDGDKVKVSVRFRGRELNYTSLGMEVLNRFAEALKDVGTVEKKPKLEGRSMVMIL
                     NPKQ"
     gene            11167..11364
                     /gene="rpmI"
                     /locus_tag="PLG72_01260"
     CDS             11167..11364
                     /gene="rpmI"
                     /locus_tag="PLG72_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOJ79464.1"
                     /translation="MPKVKTHSSSKKRFKITGTGKVKMSHAFRRHRLVSKARKAKKHH
                     RLGAYASSADEANIKRLVPYK"
     gene            11382..11732
                     /gene="rplT"
                     /locus_tag="PLG72_01265"
     CDS             11382..11732
                     /gene="rplT"
                     /locus_tag="PLG72_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ79465.1"
                     /translation="MSRVKGAVRTRARHKKVLKLAKGYFGDKSKLFRIANQAVMKSLS
                     YAYRDRKARKRDFRKLWISRINAAARMNGMSYSRFMNCLKKSGINLNRKVLADMAVND
                     EQAFAQLVESVRNA"
     gene            11820..12674
                     /locus_tag="PLG72_01270"
     CDS             11820..12674
                     /locus_tag="PLG72_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ79466.1"
                     /translation="MIRLTSSQNPVVKEIRSLRNKSGRNERGLYFIEGARFVSEALNE
                     YGAAAVIRDIKYVAVSDSFVGSGGYESIVKPCLDRGIKVYSLTDSLFESVSDTMNPQG
                     VLAVLGMKKESLKDAALRDGILVILDGIRDPGNMGTIIRTADAAGCAGIIVTDGCVDL
                     YNPKVLRSTMGSVFHLPVLHCRSTAEAVDICRRKGFLICASHMEGSVSIYDADLSGNV
                     AVVIGSEAEGVCDEVLRNSDMLIRIPMTGRAESLNAAVAAAIIIYEAVRQKSYTEMKE
                     RAMFKTGV"
     gene            complement(12754..13152)
                     /locus_tag="PLG72_01275"
     CDS             complement(12754..13152)
                     /locus_tag="PLG72_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HOJ79467.1"
                     /translation="MKYGITIRIIVLLIVITAFIILSGFFALDLIRDDSEKLEKNIGS
                     VQNSINAGNWGNASEALDRIKNDWMDIKKTWSALIDHEEIDNIDITIAKLEAMLEAKD
                     SVSALSEAAALRRLVTHIPEREKLSLENLF"
     gene            complement(13149..13844)
                     /locus_tag="PLG72_01280"
     CDS             complement(13149..13844)
                     /locus_tag="PLG72_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOJ79468.1"
                     /translation="MLVVFVRALVLYILVVIVMRIMGKRQIGQLQPFELVIAIMISEL
                     AAVPMQNTGIPLVNGIIPILTLLAIQLLISFLSIKSIRFRSLICGKRTVLIENGKIRE
                     DALRNELYTLHDLLEQLRINNCPNISDVEYAILETNGQLSVIPKPQKKPLTPGDMNIQ
                     AQAEGIIVDLIIDGRIMSENLKIAGKDRKWLQSQLHMNNFSGPDDIFYACMDTATQQV
                     FFQPREKGVEKTK"
     gene            14005..14202
                     /locus_tag="PLG72_01285"
     CDS             14005..14202
                     /locus_tag="PLG72_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79469.1"
                     /translation="MSLTTKELMLLQDNIKMAENSIKFMQACAESASDAQVKGLCQSL
                     VREHQQDMQTLIRHITNTQMQ"
     gene            14216..14491
                     /locus_tag="PLG72_01290"
     CDS             14216..14491
                     /locus_tag="PLG72_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOJ79470.1"
                     /translation="MAQLSDKEIMYTLLNEHKLCASSLTNLILESSNQALRNDAAGIL
                     NRTFQHQKTIFDIMAQKGWYTVQQASQQDIYNARNETEKIQSTIGQI"
     gene            complement(14738..14941)
                     /locus_tag="PLG72_01295"
     CDS             complement(14738..14941)
                     /locus_tag="PLG72_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOJ79471.1"
                     /translation="MANNTSRSNSRTSFDQLKYEIASQVGVNLKQGYNGDISARDAGK
                     IGGNIVKKVFESYTGSNYDINKK"
     gene            complement(15008..16555)
                     /locus_tag="PLG72_01300"
     CDS             complement(15008..16555)
                     /locus_tag="PLG72_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ79472.1"
                     /translation="MDGNVIYSDEYIKIFTSNNDVLAETYKKGFSVEQLFPVLEKHPQ
                     VTVTNMDTLRSLLLAAPAGPKKIGEFRNRIQLEIFPDVLTATITFYMTKEELSRIPAD
                     NLRVAVESLLKQNGVVFGINLDFLYGELEPFRPYTAARGIPAEDGKDAVIKMYELAEA
                     KPQVAEGGKVDFYEMKLINRVKPGDWLGERIEATEGTPGKNVKGEVIEPVKGKTAPLL
                     YDKRTVEELQTPGKTVLVSRINGAVNYEDGRISVSNHLEINGDAGVATGNIKFDGYVT
                     IHGTINDGYSVEATNDIEINSPYGLGNVKSITSTNGSIYIKGGVSSKNRVEIIAGKNV
                     YVKFADNARIVCGGEAHIGYYCINSEIVAKGVLFDSFNGQVIGGNIRAEVYISVPVCG
                     SEIERKTVLEVTGFNRQALVRRLDELFLEISSKKLELQKLKSHPSETSYLSEKLARLR
                     EELRTLEEERKLIAMYLNKKGDGEISITKRIYPNSYIILGDMYADAEPQAMPQTFYLK
                     DGEIKKL"
     gene            16937..18274
                     /locus_tag="PLG72_01305"
     CDS             16937..18274
                     /locus_tag="PLG72_01305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ79473.1"
                     /translation="MAIVYILTTGFALLPMEHGAYGNVGSASGTDTRASSKTSVHDGD
                     ISGQGKKSRVLRSIDRASSDDARVFPAAGGILDVTVTTGETDPDIEPPSINAMAAVVV
                     EESTGRILYEKNALQKRSIASTTKIMTALVALENANVEDVVTVSKRAASIGGSVAGLR
                     YGEKYTLRELLHALLIVSANDAAIAIAEHVGGSVEEFAGMMNKRAKSLGAVNSNFVTP
                     HGLDTENQYSTAYDVALITIEALKQPLFREIVSTVSSYIPGHNLHNTNELLGTYPGAD
                     GVKTGYTGKAGRCLVTTAKRNGMRLISVVLGSPTRSARAEASRKLLDYGFENYRMYRL
                     MSKGDVYAKIPVYRGVTEYVELKADRDIVLPLNRKEAESLEVKEHVPDVVGAPVYAGS
                     VSGYVEFVLDGRVLAQNVLKAGEDIRKTNYLDYFELIFRTWGRMIREGIFASL"
     gene            18870..19520
                     /locus_tag="PLG72_01310"
     CDS             18870..19520
                     /locus_tag="PLG72_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOJ79474.1"
                     /translation="MRKIYDTSEEKGSITVEASIIIPLVILSIAAAIYIGMLLYQKAL
                     LQSAAGAAAEAGAAVWAEGTDALESYRPDRGMESFRLYRRIYDRGAEDRLRKIEEYAL
                     SLASRNELVKSEGSETEAVLRDNIICRKLEVTITKYYRIPLGRFVKIFGGSDKVRITA
                     KAVSTVSEPAELIRTTDFIIDLEKKLEDRYPGLKKAGEKTRETMNELKERIERFAD"
     gene            19593..22025
                     /locus_tag="PLG72_01315"
     CDS             19593..22025
                     /locus_tag="PLG72_01315"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HOJ79475.1"
                     /translation="MKRSVSGQTTVFSAIIMVAVLVLAGLLVDAARISLGRIMVKRSV
                     EMAANSLLAEYSSKLKDDYGLFAMPVSDKYELQDRFQEYLSCNLGIPCDDEHYYGNAD
                     LFGFRIERVDVTPVYNLSENSITKKQILEYMKYRAPTELVEDFLDKLTAMKDLGRMSE
                     AYRQKVGIDKLLGSMDKSQQKLKKHIDGLGGSADKFVNGFNSGGSWVSAFNEFNSQTD
                     VLSAIESSLGSLESSIDSLEKQLDDMGIKTGSDVDQPQDSTSGHTDSRNEIKQQIAGL
                     KEEREQLGKVRADTINRLNDLWDMIRNPLTSDYLNANRAAMDEVSKIAQKGEKAQAAI
                     ARLEEFLNDSFSGGEGTFSQGFRDQLSTELAEMKEIILEGQKAGMMIEQISGNISTLE
                     KIIGEMDEIRKNCGGSLPADGLPARLIDMIGSYTNIDYDYSRPEKGDKKDDPRKGKAD
                     EVKRFISGELLNDVNYETAGYDKESLPSHKKAVTENFDERDMEFIGYSAASASTAESV
                     QTVVSYKGDFGNVGDDADLYNEESEFQENALGFISGIGSLVSDQAAALRDSIYINEYI
                     MGTFKNSVPSLVNGTETVRDTNLHGDEKEKTETFYDSEVEYILHGQPSQKLNNMMTRG
                     ELLLVRFGLNTLHVYTDPAKRTKAATIASAAAGWWTGGAGIPLISNLIMAGWSLGESV
                     IDVTDLLEGRSVPIYKMKGDWRLDIGIASEAGPKTDRRLYFNYHDYLRLFLLTVSDDT
                     KLSRIEDLIQLNICNAMGSDDFRMSECHTCVRIEAEVSMKYLFITQPFIRNELKTGDG
                     RYIYKVLLYEGY"
     gene            22119..>22817
                     /locus_tag="PLG72_01320"
     CDS             22119..>22817
                     /locus_tag="PLG72_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOJ79476.1"
                     /translation="MHRKTVLSKTSVSKGAVTLEAAIVLPVVLCAFFSVVFILRAAYT
                     YSLIQHALSETALEMASSGYIYHISGIRDLHDTVRDTINDRTELMEDQAGSVADAFGY
                     LFGSGADSLLNTGGDPAGPGLEDVEENFSRIFDYTKDIAADPLDEIKTVVCCIAGGAF
                     DDMKTQLFIPIARLYMKKYLVTDDMTDVEERLRSLNVVGGFSGLDFSESSFLSDRNED
                     IDIVVRYRIRLPLPI"
BASE COUNT         6298 a         4865 c         5975 g         5679 t
ORIGIN      
        1 aaagagatag acaataagag gaaagaattc ggcttaccgc tgagggatgg gggtaaataa
       61 atgtctgctg tagtggaaaa agtatcacag cctgtacagg gaaagaccgc aacattcaaa
      121 agcaggttcg aaaacctgaa aaagcaggta ataaagcata aggtatctta cctgttcgtt
      181 gcgccttttg gaataatatt cctggttttc accatcatac ccgtagtggt ttcgatggga
      241 ctcagcttta catacttcaa tatactggag acgcctaaat tcataagctg gcagaattac
      301 ataaggctgt tcctgggtga cgacgtattc ctgaaggctg tcaagaatac tttcctgctt
      361 gcggcgatca caggacctgt cggctatctt gcatcactga tgtttgcgtg gtttatcaat
      421 gagctgcccc cgaaaataag ggcattcatg gtcctgattt tttacgcgcc gtccatatcg
      481 ggcaatgtat acatgatatg gcaggtaatg ttcagcagcg atgcctatgg ttatgtgaac
      541 tctacactga tcaagctggg catcataact gaaccgatat tgtggctcca ggatccgaga
      601 tatgtaatgc ctgttatcct gatagtcgtg ctgtggatga gtctcggcac aggcttcctg
      661 tcttttgtcg cgggtctgca gagcatcgac agagcccagt atgaagccgg gatagtagac
      721 ggtgtgaaga ataggtggca ggaactctgg tacattacat tgccgaatat gaaaccgatg
      781 ctgatgttcg gtgccgttat gtcgattact tcatcctttg ctgtaagtga tgtcaccata
      841 gcgcttgccg gtatgccgag cgtggactat gcagcgcata cggtagtcaa ccaccttatt
      901 gactatggta ctatacgttt tgaaatgggt tatgcgtcag ctatagctac ggtactgttc
      961 ctgacgatga taatctgcaa caaggtggta cagaatcttc tgaagaaggt ggggagatag
     1021 tatgaagcat cggaaactca acacaaagag gctgaaccgt tcattggggg gagatatatt
     1081 cagcatagtg atactggtca tattcggctc attcatggtg cttccaatga tatatgccat
     1141 ctgtaacgca ttcaaacctc tggatgagct gttcatgttc cctccgaggt tttttgtgcg
     1201 caatcccacc cttgataact tcagggatct gttcttgctg atggcccagt catgggtgcc
     1261 tttttcccgg tacatattca ataccctgtt cattactgca gcaggtaccg caggacatgt
     1321 cgtggtagcg tccatggcgg cttatgcgct tgaaaagcat aagttccccg gatcgaaact
     1381 ttttttcagc atagtggtca cttcgctcat gttttcttcc gttgttacag caataccgaa
     1441 ttacctggtg atggcaaaat tacactggct ggacacatat cttgcattga tcgttcctgc
     1501 tttcggatat ccgcttgggt tgttcctcat gaaacagttc atggagacgg tccccgattc
     1561 tctcctggaa tcggcgcgta tggacggcgc aagcgaattc aggacgttct ggacggtagc
     1621 gatgccatgc gtcaaacctg cctggctcac actggtcatt ttctcgttcc agaccctctg
     1681 gggcaatact ggaagcacgt ttatatacag cgaagaattg aaaacactgc cgtatgcact
     1741 gaaccagata ttgctcggcg gatttgccag ggcaggagtc ggcgctgcgg tcgtactcct
     1801 tatgatgata gtgccggtag ttttgttcat ggtcacgcaa agcagcatca tccagaccat
     1861 ggccacatcc ggtataaagg aatagagggg agggcaggac attgaggctc aggaagatat
     1921 tgacggctgt tattatattc attctgcttt gtccggcaac cgcttatgcg gatgaaccct
     1981 acaacaatta taattatgac ttttggggga ccccggtacc ggcgcctgca gcgtatcttc
     2041 cgtcgaggat aatcgacggc aggtctcttg gtacatacga attcagaaaa ccccaggatc
     2101 ttttcacagg tccggataac aagctatatg tagcagatac aggcaataac cgtatagtgg
     2161 tcataaatga atactgggaa ctggacagga taatcgacgg gtttgacaac aacgggtcac
     2221 aggatacttt caataacccc cagggattgt tcgtgaccaa tgacggagaa atatatgtgg
     2281 cagatacgag caattcaagg atagtccacc tggacgctga cggtaatctt atcaggataa
     2341 taggccgtcc tgtttcagat attatccccg ctgatctcca gtattacccc actgcgctgg
     2401 tagtagacaa ggctggaagg atatacataa tagcccaggg tgtcaaccag ggtctgatag
     2461 aactggacaa ccagggcgaa ttcgttggct atgtaggagc caccaaggtc aaattcaata
     2521 tggtcgatta tttctggaaa ctcatagcga cggatgagca gagagaaagg atgcaattgt
     2581 tcgtaccgac agaatacaac aacatcgaca tcgacacgga aggattcctt tatgtcacga
     2641 ccagcagcct ggcgctggaa gatattgatc gggcgataaa cacgaggtcc aaggacgaca
     2701 ggtacgctcc catcaggaag ctgaacccga ccggtacaga tgttttaagg agaaacgggt
     2761 atttccctcc tgtgggagat gtgtatttca gatcgcgtgg ttacgctgaa tcaggcaatt
     2821 cccagttgta tgacgtttgt gtcgaggatt cgggcattta cagcgtgctg gacaagaaga
     2881 agggaaggat attcacatat gatacggacg gcaacctgct gtaccttttc ggaggtatag
     2941 ggaaccgcct gggcaacttc agggatccgg ccgctctgga taccatcaat gaccagtttg
     3001 tcgtgcttga ctcgaacatg ggccaggtca cggtatttga accgacactg tacggccagt
     3061 tggtcaaaga agcggttgtg ctccatctga acggcaggta tgacgagtct gcggcaaaat
     3121 gggagcaggt gcttaagtac aacgcaaact cagaacttgc atatataggc atgggcaagg
     3181 catatctgag gaaagacgag ttcaggctgg caatgaaata tttcaagctc gggaacaaga
     3241 gggattatta cagcaaggca tttgaccttt acaggcagga agtgatcgga gacaatttcg
     3301 gatggatcgt aatatcgatc ctggtgctgc tggtcggatc gaagatcctg aagaaggtcc
     3361 gggccaggaa aaaggccgga atcaaagcca tgggaaaggt ggaagcatag atggaagtat
     3421 tgcaaagtct gagatattca ttgtatctga tatttcaccc ttttgacggc ttctgggatc
     3481 tgaagcatga aaagaaagga aatctcaaga cagccctgct catagtcgct atggtcattg
     3541 tcacgtttat actccgcagg cagcttacag gattcatcct gaatgagaac aaactgcggg
     3601 aactgaatat cattgtcgag atattgagcg tagctttgcc gttcttcctg tggtgtttgg
     3661 ccaactggtg cctgactact ctgatggacg gtgaagggag cttcaaggat atagtgataa
     3721 cgagcgccta tgcacttgtg cccctggttt tgatcaacat accgcttatt gttttcagca
     3781 atgtgattac gcttgaggaa gcagctttct acacgttctt cgatatattg tcgcttgcat
     3841 ggaccgtcct gctgctgttc cttggcacga gcgtggtgca tcagtattcg ttgttaaaaa
     3901 cattcctgac cagtatatgt attgtgatag gcatggggct gatcatattc atagcgctgc
     3961 tgttcttcag cctgctgcag aagatgtatt ccttcgttta tacgatatac aaggaaatcg
     4021 cactgagact atagggggtg taaagaatgt ccatgaaaaa gataacagct ttgttaatag
     4081 caaccgctct tcttatgtct tgcagtgccg tttttgctga tcccggcgtc ggccaggccg
     4141 gtggggaaac gctgcagtcg atgaagatgg tcgcagagaa cgattatctt gcgctttata
     4201 tcaatgagga aacgactgaa gtcgcagtga aggacaaaaa aacgggagtc gtgtggtata
     4261 cgaatccgcc ggacaggagc gaggacatga tcgcatccgc gatcaacaag gataaactgt
     4321 ctgcccagat atcgatttca tactttactc ccacatcaca gcaaaaactg atgaacaact
     4381 acacggactg cataaagtat gggcagtttg aaataactgc gattgaaaac ggtgtcagga
     4441 tcgtatacag gatcggcagg gaagaaaaga tttatatact tccccaggtg atcagcaagg
     4501 agaggatgga agagaagatc ctgaaaaatc tggataacgc gaccggcaag aaactggtca
     4561 acaggtacag gctcatatcg ctggaaaatg cgaaatccga aaagcagcgg caggaaatgc
     4621 tggaagagtt tccgactgta gcgaacgggg acctgtatgt ccttggtgac aatatcaagg
     4681 atttcatgaa agtgcagctg gaggagatag tcatatcggc aggatatacg ctggaagata
     4741 tgaatgagga tcatatagaa aacaacgttc ccccggccga agagaacaag gagcatttcg
     4801 tcataccgct ggaatatacc cttgatgggg acaatctggt ggtcagggtc ccaactgacg
     4861 agatcgagta caacgcggat tcgtacccca tatatacgat gtcgctgctg gagttcttcg
     4921 gggcagccgg gactgatgaa agaggttata tactcgttcc ggacggatcc ggttcactga
     4981 tatacctgaa taacggcaag ctgaatgcgc aaacctatgc catcgatgtt tacggcaccg
     5041 atcagtcgat accgctggcg gtaaaaacga gcatcatcga acaggcatac cttcccgtgt
     5101 ttggcatgaa gagaggagat aacgcgtttt tctccataat agaatccggt gatgctgtgg
     5161 cgagtgtctg ggcggatatc agcggcaggt tgaactcata taattatgtg tattccgctt
     5221 ttacactcgt tcagaatgat gtgctggata taggcgatta ttccggcaac aatacgataa
     5281 tggtctacca gccgagagta ttcaaaggtg acgtaaaagt caggtataaa ttcctgaagg
     5341 gcgaagacgc agattatgcg ggcatggcgg cttactaccg cagttacctg gaaaaacagg
     5401 ggatgaccag ggaaaaaccg cgtgataatg tcccgttctt ccttgaagtc gtgggtgccg
     5461 tagacaaggt caaatccata ttgggtctgc ccattaagac tgccgaggta cttactacat
     5521 atgaacaggc aatagagatc ataaatgccc tgaatgatgc gggcatcagc gaaataatcc
     5581 tcaaatatac ggggtgggca aatggcggta tcgaccactc cataccaaag agcctgaagc
     5641 tgatgtcgaa gctgggaggg agcaagggct ttaagaagct gcagtccttc cttaatgaaa
     5701 aaggcattga gtatcttcca gagcttggat tcatactgaa ctacagggat aagctgtttg
     5761 acagctttat acccatgatc catgcttcgc gctacataac taaggaaatc gcctcgatat
     5821 ataaatataa ccttgctacc aatgtgcagc aaaggtccct tggaactttc tacatcatca
     5881 gtcctacaaa gattccgtct atcgtggacg gcatgatcaa aagccttaaa aaactggatg
     5941 tgacaggcat atccttaaga gacggggcaa aaaacctgaa ttcggattat aaagagtcgc
     6001 gtcttgttga caggcagcag gcaagggtga tcgtccagca gcagatcgag aagatccatg
     6061 agtcagggct gtcggtaatg gcagagggag gaaatgccta cgcgctgccc tactcggatt
     6121 atatccttga tatacctgaa gaatcgaacc agttccatct tacggatgag agcatacctt
     6181 tcttccagat ggtggtccgc ggttatatca catatgcggg gacaccgata aacttgtctt
     6241 ccgattacag gaagactttc ctgaaatcca tcgagaccgg ttcaggtata tacttctgct
     6301 ttatctatga ggaaaactca ttggtgaagg aatcatactt cgatagctat tactcaactg
     6361 agtacaggtt atggatgaat gaagccaaag agtactataa aaaggcaaat gaagctttga
     6421 gcgatgtgca gggacagacg atcaaaaacc atgaaaaact ggcagaggga atatataaga
     6481 ctactttcga aaaaggcaag gaaattgtag tcaactatac accaagacgt gtggaaatcg
     6541 acggaactac ggtggaacct cttgatttca aagtggttaa ggagggtcaa tgatgaggga
     6601 ggcaacaatg acaaacagag cgctgaaatc agcagccgct ccgcagaaaa agaaaagaag
     6661 gggactggat cttaccagaa aaagggccgc gatgggttac ctgttcgtgc tgccttttat
     6721 aatcgggctc atcacatttt tccttgtacc tcttgtacag tccttcatat tcagcctcaa
     6781 taagctggaa gttgcggaag gcggctacaa tctcataccc atggggtttg aaaactacaa
     6841 cagggctttg agggtccatg cctcctacgt gagagacctg tgggaatcag tcagatatat
     6901 gatcgccaac gttccattga tcatcatatt cagttttttt gcggctaatc tgctgaatca
     6961 gaaatttaaa ggccgttcac tggcaagagc aatatttttc ctgcctgtca tagtgacgtc
     7021 aggagtaatg ctggtcgttg acagcggaga cctgctgcag tcttcacttg gcatgacgca
     7081 gacagatggc acggagggag cgttccgggc agtggaactg gcaaggctgc ttctgcagac
     7141 caggttgagt gaaagattca tcgattatat catcggcgcc gtggatgcga tatatgatat
     7201 cgtttcggca tcaggcgtcc agatccttgt tttcctggca gggcttcagt cgatatcccc
     7261 ttcgttattc gaagcttcga atatcgaagg agcaacaggc tgggagaatt tctggaagat
     7321 cacgttcccg atggtgagcc cgttgatatt ggtcaatacg gtatacacca tcatagattt
     7381 gctgatcaat tcatcaaata cagtaatgga gctgatcagg gatacagcgt tcgcgtcgca
     7441 ggattatggc atgagttcag ccatggcctg gatatatttt gctgctatcc tcctgatcgt
     7501 gggcctggtg atgtggatag tctcgaaact ggtattctat tacgattaaa aggggtcgga
     7561 ggtctttatg aaaataaagg gaatattcca caggaaaagt aaactagatt ataaagattt
     7621 acagcatagg aagcatatga tgtatacatg ggggaaacga ggctggtcga tcgtaagagg
     7681 ggtcattata gccggaatat gctttctcat tctgtacccc acactggtaa agctgtcagt
     7741 ttcattcatg ccggaaaaag atatatatga tgtgaccgtg cggtatgttc cgaagtcgcc
     7801 gacccttgaa aactacaaga ccgttatcaa ggcaatgaaa tatggcaccg cgttctggaa
     7861 cacgttaaag ctggccctgc tgacaagttt gatgcagctg atctcatgta cattgatagg
     7921 atatgggttc gcaaggttca aattcaaagg aagaaacttt ctgttcgcac tggtaatcct
     7981 gacgatggta gtgcctcctc agactttgat gatcccgttg ttcctgcatt tcaggtactt
     8041 tgacatattc ggcatcattt cagctgtcac cggaggaaag ggcataaacc tgctggaaag
     8101 ttactggccg ttcatattga tgtcgatgac agggatgggg ctgaaaaacg ggctgtacat
     8161 atatatcatg cgccagttct tcaggaatat gccgaaggaa ttggaggaag ccgcatatgt
     8221 cgacggtgcc ggcatgctga ggacatttgc gcaaatcatg ctgccaagtg ccgtaccgtc
     8281 tatggtgact gtgttcctgt tctcattcgt atggcaatgg acagatacgt tctattcgac
     8341 attattcctg caaaagacaa gagtgctggc atctgcactc agcagtgtct cgtttgacgt
     8401 gacccagaag atttactttg agacaggagt gaatgtggca ctggctccgg cgatcagttc
     8461 aatgtatacg aacacggcga gcctgcttgt cgtactgccg ctgctgatac tatatctgtt
     8521 cgcacagaaa cgtttcgtcg agagcgtcga gagaagcggt atagtgggat gatgacgggc
     8581 catacagcgc ggaaaacagt tcaggtggct gaagccggga actgcgccgg aaaccgtatt
     8641 atattgacac tatggtgtgt ttttggtaaa ttcttaactg gaagcaccca tatattgtgg
     8701 ttggaaatac cggaaaagac aacttcttca tctgatttgc gattaaaacc cattgggttt
     8761 taataaataa aaaatattat agggaggtta cattatgtca agaggtctta aaaagttatt
     8821 ggctctgagc ttggtaacag ttatgatggt agctgttatc gcaggctgcg gccagaagac
     8881 tggaacgacg ccgactgaca caacgccgga cgaagttgaa ccggcaccgg ctgaacagac
     8941 tccgtcagaa ccggcagatg aagaacccga acctgaaccg gaggagatca gggatcttgg
     9001 cggcagggtt atcaaagtcg cagcatggtg ggatctgact cctcagggag gaactccgag
     9061 cggagaccgt caggtagccc gcagagagga aatggagcag aagtacaact tcaagttcga
     9121 gtatgtgaat gttccgtggg atcagaccgt tgaaacctat tcatcttcag ttatggcagg
     9181 agatccgttt gcagacatcg caactctcga agacaactgg gtgatcggac ttgccaacag
     9241 cggatttgtt acagagcttg attcgttgtt tgattttact gatgaaaaat gggaccaggt
     9301 aaccaagagt ctgtcaacaa taaatggaaa gacctatggc atgggaaccg gcaaatggtg
     9361 gccgcgagga atagtcttct acaacaagaa catcttcgaa agagaaggct tggagaaccc
     9421 ctatgaactg atgaagaacg gtcagtggac atgggaaaag ctggaagaga tcgcaaggaa
     9481 agcaacgaag gacactaaca acgatggtaa gatagatcag tggggccttg gcggtatgga
     9541 tatcgactgt tcactggtca tatccaacgg ttctgagttt gcaagcattg tagacggaaa
     9601 ggctaaactc aacttcaggg aacctgcagt tctcgaagca ttcaacttct atcagagact
     9661 ctgcaaagag ggcatcatat tcaacaagtt caattatgaa gctgaggaga taccttggga
     9721 ccttgcagcc aacgtattca aagaaggcaa gatagcaatg ttctggtacc agtactggaa
     9781 gatagacgat ttcagagaca atatggctga tgactatggt ctggtactgc ctccgatagg
     9841 gcccagcaat acagagaaga aatactactg catgatttca ggccataatt tccagactat
     9901 cccgaagaat gtaaagaacg tcgaagacat tgcattcatc tggaacaagt ggaccgaacc
     9961 gttcccggag gatctggaag atcctgacgc ctggatggaa ccgcatatga acaacgtacg
    10021 tgatcaggag tccctggaag tactcaagta catgtacgat aataactgcg gcaagcacgt
    10081 cggcctgttc ggagccgttc ggcctgctgt cgaattgtgg tggggcggcc aggacgacct
    10141 gctgaaaggc gagaagaccg ttgcgcagat aatcgatgag aaatatgacg ctatccaggc
    10201 agcactggat gacttccttg ccaacaagta acagggacgg aattaacaga aggtacgcac
    10261 gggaaatcaa atcaggatac gtaaaccaaa gcttccggcg aataaatagt tcgccggaag
    10321 cttttttctt gacatatatc attgtttctg ttatcattat tgcggtaaaa caaagaagaa
    10381 gccttccgct tctcacctta cggacatgtc cgtcagggtc aataatgatc aggatcatta
    10441 tgacgttgct tttttcagcg gatcggaggc tcttctttcg gtccgctttt tatatgccgt
    10501 accggatgca aggccggggc attaaaagac cgcgaagcgg attgcgaatt gctggggacc
    10561 atattaacac tcggaggtgt atagatatta gaaaaagtga ttatatgatc aatgaggaga
    10621 tcagggacaa ggaaatacgc ctgatcgatt ctgacggtac catgcttggg attatgtcag
    10681 cgaaggaagc cctgaaaatc gcgacggaaa aggacctgga ccttgtaaag atcgcaccgc
    10741 aggctgtacc acccgtatgc aagctcatgg attacgggaa gtatatgttt gagcttgcaa
    10801 agaaggagaa ggaagcaagg aagaaccaga aagtcgtcac catgaaggaa gtctgggtaa
    10861 aaccgaacat cgaggatcat gacttcgcgt tcaaatcaag gaatgcggcc aagttcctga
    10921 aggatggcga caaggtgaag gtcagcgttc ggttcagggg aagagaactg aattatacat
    10981 ctctgggcat ggaagtgctc aacagattcg cagaagcttt gaaagacgtt ggaacggtag
    11041 agaaaaagcc gaaactggaa ggacgcagca tggtcatgat tctgaatcct aaacagtaag
    11101 gcatttcata aggcatccgg tcaacggaag ctgccggatg cgaaacagaa aacaggagga
    11161 atggatatgc caaaagtcaa gacacacagt tcatccaaga aaaggtttaa aataaccgga
    11221 acaggcaaag taaagatgag ccatgctttc aggagacaca ggctcgtttc caaggcaaga
    11281 aaggcaaaga aacaccatag gctcggagct tatgcttctt ctgccgatga agccaatatc
    11341 aaaaggcttg taccgtacaa gtaggaagga ggaagatcga tatgtccaga gttaaaggtg
    11401 cagtaaggac tcgcgcaaga cacaagaaag ttcttaagct ggcgaagggc tatttcggcg
    11461 ataagagcaa gcttttcagg atagccaatc aggctgtgat gaaatcgctt tcgtatgcat
    11521 atagagacag gaaggccaga aagagagatt tcagaaagct ttggatatca aggatcaatg
    11581 ctgccgcaag gatgaacggc atgagttaca gcaggttcat gaactgcctc aagaagtccg
    11641 gcatcaacct gaacaggaag gtacttgccg atatggcagt caatgatgaa caggctttcg
    11701 cacagttggt cgaaagcgta aggaatgcct gaataacgaa atatacggaa aggcaaggat
    11761 agggggcgta accggccctc tattgtttta tgaggaaata atattttcga ggggagaaaa
    11821 tgataaggct taccagcagt caaaacccgg tggtgaaaga aatccggtcg ctcaggaaca
    11881 agagcggcag gaatgaaaga ggcctctatt ttatcgaagg cgcaagattt gtatctgaag
    11941 ctttgaatga atatggcgca gccgccgtta tacgtgatat aaagtatgtt gcggtatctg
    12001 attcattcgt cggaagcggc ggttacgaaa gcatcgtgaa gccctgcctg gacaggggaa
    12061 taaaggttta cagcctgacg gacagcctct tcgaatcggt ttccgatacg atgaatcccc
    12121 agggcgtcct tgccgtattg ggcatgaaaa aggagagcct aaaagatgcc gcactcaggg
    12181 acggcatcct ggtcatcctt gacgggatca gggatcccgg gaatatgggt actatcatca
    12241 ggacagcgga tgcagccggc tgtgcaggca taatcgttac ggacggctgc gtcgacctgt
    12301 acaatccgaa agtattgagg tcaacgatgg gttcggtttt ccatctgcct gtcctgcatt
    12361 gccgtagtac tgcggaagcc gtggatatat gcagacggaa gggtttcctg atatgcgctt
    12421 cccatatgga aggctcagtg agcatttatg acgcagacct ttcgggcaat gtcgccgttg
    12481 tgataggcag tgaggctgaa ggtgtctgtg atgaggtcct gagaaattcg gacatgctga
    12541 tcaggatacc gatgacgggc agggcggaat ccctcaatgc cgctgtcgcg gcggctatta
    12601 taatatatga ggctgtcagg cagaagtcat atacagaaat gaaagaaaga gccatgttta
    12661 agaccggcgt ataaactgca taagagatgc gccatgcctc ggcgctgcag gaaactggcg
    12721 gaagatcgaa gtatgaattg ccggacaatt gacttaaaac aaattttcta agctcaattt
    12781 ttcacgttcg ggtatgtggg tcacgagtct tctgagagct gcagcttcag aaagggcgga
    12841 gacggaatct ttcgcttcca gcattgcttc cagctttgct atcgtaatat ctatattatc
    12901 gatttcttca tggtcgatca atgctgacca tgtttttttt atgtccatcc agtcattttt
    12961 gatacggtcc agagcttccg aagcatttcc ccagttgccg gcatttatgc tgttctgaac
    13021 cgatcctatg ttcttttcca gtttttcaga atcatcacgg atcagatcca gcgcgaaaaa
    13081 gccggatagt atgataaatg cagtgatgac gatcaacagc acgattatcc ttatcgttat
    13141 gccgtatttc atttggtttt ctcaactcct ttttcacggg gctggaaaaa tacctgctga
    13201 gttgcggtgt ccatgcaggc gtagaatata tcgtccggcc ccgaaaaatt gttcatatga
    13261 agctgggact ggagccattt cctgtccttg ccggcgatct tcaggttctc gctcattatg
    13321 cggccgtcga ttatcaaatc aacaataata ccttcagctt gtgcctgtat gttcatatcg
    13381 ccgggcgtca gcggcttttt ctgcggtttt gggataacgc tgagctgtcc gttggtttcg
    13441 agtatcgcat attcaacatc ggagatattg gggcagttgt ttatccgaag ctgttcgaga
    13501 aggtcgtgga gtgtgtacag ttcattcctg agtgcgtcct cgcgtatttt gccgttttct
    13561 atgagtactg tccgctttcc gcagatcaaa gacctgaacc tgatgctttt gatcgataaa
    13621 aatgaaataa gcagttgtat cgccagcagt gtcagtatcg gtattatgcc gttgacgagt
    13681 gggatcccgg tgttctgcat aggaacagcg gcaagctccg agatcattat cgctatgacc
    13741 agttcaaaag gctgaagctg cccgatctgc cttttgccca tgatcctcat gactattacc
    13801 acaagtatat acaatacaag tgcgcgtacg aatactacca gcatttttca acctcctgac
    13861 cacatcggtt attgtctgca atgcctcaca tggttatgcg ctgtgatgat accgatgcgt
    13921 aatgcataat ttatctgtct gaatattttg cttccggttg tgcaagctat gagcgagtaa
    13981 aaaactgaag gagggttggg atacatgtca ctgacaacaa aggaactgat gctgctccag
    14041 gacaacatca aaatggcgga gaactcgatt aaattcatgc aggcgtgtgc cgagagcgcc
    14101 tctgacgctc aggtaaaagg gctgtgccag agccttgtca gagagcacca gcaggatatg
    14161 cagacactta tcagacacat aaccaacacg cagatgcaat aaggagggat atgcaatggc
    14221 acagttgagc gataaggaga taatgtacac tcttttgaat gagcataagc tctgcgcatc
    14281 atcactgacg aacctgatac ttgagagcag caaccaggct ttgcgcaatg atgcggctgg
    14341 catcctgaac aggactttcc agcatcagaa aaccatattc gacatcatgg cgcagaaagg
    14401 ctggtacacg gtccagcagg caagccagca ggatatttac aacgccagga atgaaacaga
    14461 aaaaatccag agtactatcg ggcaaatatg atatttgccc gggtataaca caaccgtccg
    14521 gtaaatttgc agatgcagga aatattacgg gataggctgc gtatgtttga gaacatagcg
    14581 tataccgaaa tcatcttgcg gatatcatga tggagccgct ccggacaata aaaagggaag
    14641 ccgtaccagt gtgcggtttc ccttttggtt atctcaatcg ttccggctgc ttaccggctg
    14701 ttcgtaaagc agcggctgac tctgccagcc gggattgtca ctttttgttg atatcgtaat
    14761 tgcttccggt gtacgattca aagaccttct tgacgatgtt gccgcctatt ttgcctgcat
    14821 ctcttgcaga gatgtccccg ttatagccct gtttcaggtt tactcccacc tggcttgcga
    14881 tctcgtactt caactggtcg aaagatgtcc tgctgttgct tctgcttgta ttgtttgcca
    14941 tatgatcacc tcctcagtaa ctagtctgcc aaatcgggag gtgattatga cttgtaagat
    15001 atggctgtta cagttttttg atctcgccgt ctttaagata gaacgtctgc ggcatcgcct
    15061 ggggttcggc gtctgcatac atatctccaa gtattatgta actgttgggg tatattcgtt
    15121 tagtaattga aatctcaccg tcgcccttct tattaaggta catggctatc agctttcgct
    15181 cttcttcgag agttctcagt tcttctctta gtcttgcaag tttttcagat aaatagctcg
    15241 tttcggacgg atgtgatttc agtttctgca gttcaagctt tttactgctg atttcaagga
    15301 acagttcatc gagccttctt accagtgcct gcctgttgaa accggtaact tcaagcacgg
    15361 ttttcctttc gatctcagaa ccgcatacag gtactgatat gtaaacttct gccctgatat
    15421 tcccgcctat gacctgaccg ttgaatgaat cgaacagcac gccttttgcc acgatctcgc
    15481 tgttgatgca gtaataacct atgtgagctt cacctccgca gactatcctg gcgttgtcgg
    15541 cgaatttcac gtaaacgttc tttccggcta tgatctcgac cctgtttttc gaagatacgc
    15601 ctcccttgat ataaatgctg ccgttcgtac tggtaatgct ttttacattg ccaaggccgt
    15661 atggactgtt gatttctatg tcatttgtcg cttcgacaga atacccgtca ttgatggtgc
    15721 catgtatcgt gacatacccg tcgaacttga tgtttcctgt ggctacaccc gcgtcgccgt
    15781 tgatttccag gtggttcgat acgctgatcc ttccgtcttc atagttgacg gcgccgttga
    15841 tcctggatac cagtaccgtc ttacccggcg tctgcaattc ttccacagtc cttttatcat
    15901 agagcagtgg tgctgttttc ccttttaccg gctcgataac ttcacccttt acgtttttgc
    15961 cgggagtgcc ttccgtggct tctatacgtt cgcccagcca gtcgccgggc ttgaccctgt
    16021 ttatgagctt catttcgtaa aaatccactt ttccgccttc ggcgacctgt ggcttggctt
    16081 cagccagttc atacatcttt atgaccgcat ctttcccgtc ttcagcgggg atgccgcggg
    16141 ctgccgtata tggacggaaa ggctcaagtt cgccgtacag gaaatcgagg tttataccga
    16201 acacgactcc gttttgcttt agcagggatt ctacggcgac cctgaggttg tccgcgggta
    16261 tccgggacag ttcttctttt gtcatataaa acgtgatggt tgcggtaagc acatcgggga
    16321 aaatttctaa ttggatcctg ttcctgaact caccgatttt tttaggacct gcgggtgccg
    16381 cgagcagcag gctgcgcagc gtatccatgt ttgtgacggt cacctggggg tgtttttcca
    16441 gaacggggaa cagctgttcc acactgaaac cttttttata tgtttcagcg aggacatcgt
    16501 tatttgatgt aaagatcttt atatattcgt cgctgtatat tacatttccg tccatatact
    16561 cacctccttt gctaatgatg atggtcctgc ggcagtattg agatgccgct gccgttatcc
    16621 cggaatgctg cttcgctgat cctgaaacac cgcttgaccg atccatgaat gacgtctcgt
    16681 ttcatggatc atgcagtgtt tttcatagga tttcctgtgt ttcaaattat tactttacaa
    16741 tatatttata cccaatttct gaccttttca tataatatat cggataattc gatatcgata
    16801 gttaacataa ttgatgacat aaatattcat ttctcgtctc atattattct tgtgtaactt
    16861 tttcagtcat gagcatcagt aacgattaac catggagatg atggaactgg gataccgcag
    16921 gataatgcta tgttttgtgg caatagtcta tatactgaca acagggtttg ccttattgcc
    16981 gatggaacat ggggcatatg gcaatgttgg atctgcatcg ggtaccgaca caagggcaag
    17041 ctcgaaaacg tcggtacatg acggcgacat atctgggcag gggaagaaaa gcagggtttt
    17101 acgcagcatt gacagggctt catctgacga tgccagggtg tttcctgctg ccggcgggat
    17161 actggatgtt acggttacaa ccggcgaaac ggatcctgac atagagccgc cgtcaataaa
    17221 cgcaatggcc gcggtcgttg tcgaggagtc gacgggaagg atactgtatg aaaagaatgc
    17281 gctccagaag cgctcaatag cgagtactac gaagataatg accgccctgg tcgcattgga
    17341 aaacgctaat gtcgaggatg tcgtcaccgt aagcaaaaga gcggcaagca taggcggatc
    17401 tgtagccggt ttgagatatg gagaaaagta tacgctgaga gagcttctgc atgcactgct
    17461 cattgtttcg gccaacgatg cggcaattgc gatagccgag catgtaggcg gttcagtgga
    17521 ggaatttgcc ggtatgatga ataagagagc aaaatctctg ggagcagtca attcgaattt
    17581 cgttacacct cacggccttg acacggagaa tcagtattcg acggcatacg atgtggcgct
    17641 tataacgatt gaagccctca agcagccttt gttccgtgag atagtatcta ctgtgagttc
    17701 atatattcct ggacataacc ttcacaatac caatgagctt cttgggacat atccgggagc
    17761 cgacggagtc aagacgggat acacgggcaa agcgggcaga tgcctggtca cgacggcgaa
    17821 aaggaacggc atgaggctta tttcagttgt actgggctcc cccacaagga gtgcgcgggc
    17881 ggaggcttca agaaaactgc tggactatgg gttcgaaaac tacaggatgt acaggctgat
    17941 gagcaagggt gacgtttatg cgaagatacc tgtttacagg ggcgtaacag agtatgtcga
    18001 actgaaggcg gacagggata tcgtactgcc gctgaaccgc aaagaggccg aatcactgga
    18061 agtgaaggag catgttccgg atgtggtcgg tgcgcctgta tatgccgggt cggtatcagg
    18121 atatgtcgag ttcgtgctgg atggcagggt gctggcgcaa aacgtgctca aagccggcga
    18181 agatatacgg aaaacaaatt acctggatta ctttgaactg attttcagga cctggggaag
    18241 gatgatccgg gagggaatat tcgcaagcct ttgagccata cagggaaccg gccgcctttg
    18301 gggacagttc tcaacttttt gaaatatgga tcgcttctca gctgtttgca tatggggacg
    18361 gttctcaaat ttcagcagac ggaaacgttt cccggcaccc gatgtcgggg acgtttccct
    18421 gatggccggt aatgggaacg tttcccggtg aacggaaaaa ctgacgatgg cgtatttctg
    18481 cataaaggtg cggaaacact gtgcttttat ccagccaatt tttcgcggcc aggccatagg
    18541 gcactataaa ttttttcatg tcgacatctg attatttcca aaattcttcc aatttcctat
    18601 attatggtgt attatattca tgcatgttat attatggcag aacaggacga tattaacata
    18661 atcagatgtt tactgtcttg actttgcagt gtgacaggca tgtccgcgaa ttcggcgcac
    18721 aatctgttat gcagcatgat ctcgggactt cgagacagga acacaatacc tggtgcaaaa
    18781 ccggctgggt gcggttaaat gcaggagatg cccgttcaaa tgaaacaagc gagtcaggct
    18841 gccggtaaga caagcagttg aggtcggtta tgaggaaaat ttacgataca tccgaagaaa
    18901 agggcagtat tacggtggaa gcctcgatca taatacctct ggtgatcctg agcatagctg
    18961 cagcgatata tatcgggatg ctgctttatc agaaagcttt gctccagtca gcagccggag
    19021 cagctgcgga agccggcgcg gcagtatggg cagaaggcac tgatgcgctt gagtcataca
    19081 gacccgaccg tggcatggaa agcttcaggc tttacaggag gatctacgac aggggcgcgg
    19141 aggacaggtt aagaaaaatt gaggaatacg ccctgtcact ggcatcccgt aatgaactgg
    19201 tcaaatcaga aggcagcgaa actgaagcgg tcctcaggga caatatcata tgcaggaagc
    19261 tggaagtaac cataaccaag tattaccgca tacccctggg cagattcgtg aagatattcg
    19321 gaggcagcga taaagtgagg atcacggcaa aggctgtttc aaccgtcagt gagcccgcag
    19381 aactgatccg tacgacagac ttcattatcg atctcgagaa aaagctggaa gacagatacc
    19441 ctggactgaa aaaagccggc gaaaaaacga gggagacaat gaacgaactg aaggaaagga
    19501 ttgagcgctt tgccgactga agagagacgg caggacagac gagcggaaga attgcagaaa
    19561 tatgtaagtt ttttgcattg tgagggtgta aattgaaaag gagcgtatcg ggccaaacaa
    19621 ctgttttttc tgcgataata atggttgctg tactggtatt ggcaggcttg ctggtcgatg
    19681 cggccaggat cagcctgggg cgcatcatgg ttaaacggtc tgtcgaaatg gccgcgaatt
    19741 cactgcttgc ggagtacagc agcaaactca aggatgatta tggcctgttt gcaatgcctg
    19801 tttctgataa atatgagttg caggacaggt tccaggaata cctttcatgc aatctgggca
    19861 taccctgcga tgatgagcat tattacggga atgccgacct tttcgggttc aggatcgaaa
    19921 gggtggacgt gactcctgtt tataacctga gcgaaaacag cataacgaaa aagcaaatac
    19981 tggaatatat gaagtaccgt gcaccgacag agcttgtcga ggactttctg gataaactga
    20041 ccgcgatgaa ggaccttggc aggatgtcgg aagcatacag gcagaaggtc ggtatagaca
    20101 agctgcttgg cagcatggat aaatcccagc aaaaactcaa aaaacatatc gatggacttg
    20161 gcggcagtgc agacaagttc gtcaacggtt tcaacagcgg cggcagctgg gtatcggcct
    20221 tcaatgagtt caactcgcag accgatgtcc tctcagccat agaaagcagc cttggatcat
    20281 tggaaagcag tatcgacagc ctggaaaaac agctggacga tatgggaatc aaaacgggaa
    20341 gcgatgtgga tcagccccag gacagcactt ccggccatac cgacagcaga aatgagatca
    20401 aacagcagat tgccggactg aaggaagaaa gggaacaatt gggcaaagtc agggcagata
    20461 ccataaacag gctgaatgat ctttgggata tgataagaaa tcctttgaca tcagattacc
    20521 tcaatgcgaa cagggctgcg atggacgagg tatccaaaat agcgcagaaa ggcgaaaagg
    20581 cacaggctgc gatagccagg ctcgaggagt tccttaatga cagtttctca ggcggagaag
    20641 gaacattttc gcagggattc agagaccagt tgagcacaga acttgcagaa atgaaggaaa
    20701 tcatactcga aggccaaaag gcaggaatga tgatcgaaca aataagcggc aatatttcca
    20761 ctcttgagaa aatcatcggt gaaatggacg agatacggaa aaactgcgga ggcagccttc
    20821 ctgccgacgg attgccggcg cggctgatcg acatgatcgg cagttataca aacatcgatt
    20881 acgattattc aaggccggaa aaaggggata agaaggacga tccaaggaaa ggaaaggctg
    20941 acgaggtaaa gaggttcatt tccggggaat tgcttaacga tgtgaattac gaaacggcag
    21001 gttatgataa agaaagcctg ccttcgcata aaaaggctgt gaccgaaaat tttgacgaaa
    21061 gggacatgga attcatcggt tactctgctg catcggcaag cacagccgaa agcgttcaga
    21121 ctgtcgtctc gtataaaggc gattttggca atgtgggcga tgacgctgac ctttataatg
    21181 aggaaagcga gttccaggaa aatgcccttg gtttcatttc aggtatcggg agccttgttt
    21241 cagatcaggc cgccgcatta agggacagca tttacataaa tgagtatata atgggcactt
    21301 tcaaaaactc ggtgccgtca ctggtgaacg gtactgagac ggtcagggat accaacctgc
    21361 atggcgatga aaaggaaaaa acagagacct tttacgacag tgaagtcgaa tatatactcc
    21421 acggccagcc ttcacagaaa ctgaacaaca tgatgacaag aggggagtta ctgctggtca
    21481 ggttcggact caatacactg catgtctata cagatcccgc aaaaaggaca aaggcggcga
    21541 ccatagcgtc agccgctgcc ggctggtgga cgggaggcgc cggtataccg ctgatctcta
    21601 accttataat ggccggatgg agccttggag aatcggtcat agatgtaact gacctgttgg
    21661 agggcagatc agtgccgatc tacaaaatga agggtgactg gcggctcgat ataggtatcg
    21721 cttcggaggc gggcccgaag accgaccgca ggctgtattt caattaccat gactacctgc
    21781 ggcttttcct gcttacggta agtgatgata caaaactcag caggatagaa gacctgatcc
    21841 agttgaatat ttgcaatgca atgggaagcg atgatttcag gatgtctgaa tgccatacct
    21901 gtgtccggat cgaggctgag gtgtcgatga aatacctgtt cataacgcag ccatttatcc
    21961 gaaatgagtt aaaaaccggt gacgggagat acatttacaa agtgctttta tatgagggat
    22021 actgatcatt cttttcgtaa tgcggaaatg ccctcgcata aagcttaaga cggctgacag
    22081 gatacctttt ttgcaaatgt ggaaggaaag caactgatat gcacaggaaa acagttctga
    22141 gcaagacaag tgtttcaaaa ggagcagtga ccctggaagc ggcaatagtg cttcctgtgg
    22201 tgctgtgtgc gtttttctcg gttgttttca ttttaagggc agcctatacg tacagcctta
    22261 tacagcatgc actttcagaa acggctttgg aaatggcgtc ttcaggttat atatatcata
    22321 tcagcgggat acgggatctg catgatacgg taagagacac gataaatgac aggacagaac
    22381 tcatggagga tcaggccgga agcgttgccg atgcctttgg atatctgttc ggaagcggcg
    22441 cggattcgct tttaaatacc ggcggagatc ctgcaggtcc gggtttggaa gatgtggaag
    22501 agaatttcag caggattttt gactatacaa aagatattgc agcagatcct cttgatgaga
    22561 taaagactgt agtctgctgc attgccggcg gagctttcga tgatatgaag acgcagcttt
    22621 ttattccgat agccagactg tatatgaaaa agtacctggt aacggacgat atgaccgatg
    22681 tggaggaaag actgcgttcc ctcaatgtgg tcggcggatt cagcgggctt gacttcagtg
    22741 aatcatcctt cctttcggac aggaacgagg atatagatat cgttgtcagg tacaggatca
    22801 ggctgccgct gccgata
//
LOCUS       DAOSCC010000010        22608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1642_length_22608_cov_4.212522, whole genome shotgun sequence.
ACCESSION   DAOSCC010000010 DAOSCC010000000
VERSION     DAOSCC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22608
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_22608_cov_4.212522"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..872)
                     /locus_tag="PLG72_01325"
     CDS             complement(147..872)
                     /locus_tag="PLG72_01325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ79477.1"
                     /translation="MKTRLIFVRHAEALGNKIRRFHGWTNSGLTEKGHLQAERVAERL
                     KAVDIDVICSSSLQRAVQTARYIADIKGLSVITSENLREINGGDWEDVAWSELEQRWP
                     DEYEAWENRPHKLKMPNGESMEEFQERLLGEVMSIIKANEGKNICIVTHGTAIRAMLC
                     HFRSCTLEEMVNVEWCDNTAITVIDYEDGVFTIVTEGDSSHLGKDLGTIVNQDWWEEY
                     LRKSKERKDRGQSRDGHSTGSTE"
     gene            complement(889..1809)
                     /locus_tag="PLG72_01330"
     CDS             complement(889..1809)
                     /locus_tag="PLG72_01330"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOJ79478.1"
                     /translation="MSDIDLSVDICGIRLKNPVIAASGTFGFGREHADYADLNKIGGI
                     SVKGLTLRPREGNKPPRVAETPAGMLNSVGLQNPGVRYFIENEIRFLRQYSAAIIANI
                     AGNTIEEYCEMSEILSDADIDAIELNVSCPNVKAGCVAFGNTPEGIYEITSQVRKHCR
                     KPLIVKLTPNVTSISGIAAAAEAAGADAVSLINTLLGMAVDIETRRPVLANNTGGLSG
                     PAVKPIAVRMVHEVYKTVKIPVIGMGGIFNGNDAVEFMLAGASAVMVGTANLVYPDAC
                     IKVCEGIEQYMRRHGFNSVADITGKLVLNA"
     gene            complement(1870..2655)
                     /locus_tag="PLG72_01335"
     CDS             complement(1870..2655)
                     /locus_tag="PLG72_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOJ79479.1"
                     /translation="MSRLLRETITNVEKAAENIYRMTVASEYVSANAVPGQFVNIKCC
                     EGTQALLRRPVSICSVDREKGEFDIFFQVKGSGTEILAGKKIGDSLDILGPLGRGFDL
                     NISYRRIAVVGGGIGIFPLLFLLDESKAIVKRSYLGFRSSENIMLLDEFRKRSSSLET
                     ATEDGSCGIKGFVTDILERDILPGEFDMIYACGPNPMLRTVAAIAGEYRVRCQISMEQ
                     RMGCGFGACLACACKVTTDGGGWQYVRVCKDGPVFDSEDVIFE"
     gene            complement(2717..5929)
                     /gene="carB"
                     /locus_tag="PLG72_01340"
     CDS             complement(2717..5929)
                     /gene="carB"
                     /locus_tag="PLG72_01340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ79480.1"
                     /translation="MSRRNDINKVLVIGSGPIVIGQAAEFDYAGTQACRALREEGLEV
                     VLVNSNPATIMTDTNMADKVYIEPLDAETLKKIITKERPDSMLPTLGGQTGLNLAMEL
                     SESGFLEKMGVKLLGTATKAIKMAEDRQAFKDAMESIGEPCVASKVVTSTEDALDFAD
                     EIGYPVIVRPAYTLGGTGGGIADDSDQLAAIASNGLRLSRVGQVLIERCISGWKEIEF
                     EVMRDAKGNAAIICGMENVDPVGVHTGDSIVVAPCQTLSEEEYRMLHDAAIKIINTLE
                     IEGGCNVQFALHPTGKEYAVIEVNPRVSRSSALASKASGYPIARVATKIAIGYTLDEI
                     GNRTTGKTLKSFEPVLDYVVLKIPKWPFDKFVRADRTLGTQMKATGEVMAIGDSFEGA
                     LMKAVRSLELGISTLEQKIFVKYRTEEIEDKLANADDERLFIIAEALRRGISAELIYE
                     KTKIDQWFLDKISNIVRMEESLKTRTKATLDADIVRRAKEMGFTDETIAGYAGCSLED
                     ITRLRNENGINASFKSVDTCTERFDTVSPYYYSTYRQCGKTASSGNKKILVIGSGPIR
                     IGQGIEFDYCSVHSVWALKEQGYETIIANNNPETVSTDFDTADRLYFEPLTIEDVMNI
                     IEHEKPEGVIVQFGGQTAIKLVNKLSRLGVRIFGTQAEDVDAAEDRERFDKILEKLGI
                     PRPRGKTVFTLEEALAAANELIYPVLVRPSYVLGGQGMEIAYNDNDIIEFMEIINRVK
                     QEHPILIDKYLMGKEIEVDAISDGEDILIPGIMEHLERAGVHSGDSISVYPALSLGRP
                     VIDKIVDYTEKLAKALNVVGMINIQYVLYNDEIYVIEVNPRSSRTVPYISKVTGIPMV
                     NVATKVMTGRKLKDFGYGTGLYRTMDHVAIKVPVFSFEKLHDVDVSLGPEMKSTGEVL
                     GIGRNFHEALYKGIMATGMKLPSPGGGILMTVRDSDKTELPGIAEQFEKLGFTLWATG
                     NTARMLNMHGIATNAVKKIGEGSPDLLDLIASGKINLVINTPTRGRQPERDGFKIRRK
                     AVEMSIPCLTSLDTARAVLSCIRMKQQGISPDVIDLSGFS"
     gene            complement(6051..7004)
                     /gene="pyrF"
                     /locus_tag="PLG72_01345"
     CDS             complement(6051..7004)
                     /gene="pyrF"
                     /locus_tag="PLG72_01345"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ79481.1"
                     /translation="MFIDRLIAAIRKYGNPAVAGLDPKLEYIPSCIREKAFSEHGKGF
                     AGAAEAILEFNKRLIDALCDIVPAVKPQLAYYEMYGVDGIRAFAETCRYARTRGMIVI
                     ADGKRNDIGSTARAYSDAYLGKTEIDDAALSAFDTDALTVNPYLGIDGVQPFIDECAA
                     SGKGIFVLVKTSNKSSGQIQDLITMDGKHVYEKVAGLVDEWSMAAMGENGYSSIGAVV
                     GATYPEQACVLRKIMKRSYFLVPGYGAQGGTARDAACAFNDDGLGAIVNASRSIMCAW
                     KSEAWLEKYGESGFDHAAREEAVRMREDIRRALEERKGQQR"
     gene            complement(7018..8304)
                     /locus_tag="PLG72_01350"
     CDS             complement(7018..8304)
                     /locus_tag="PLG72_01350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOJ79482.1"
                     /translation="MKLLIKNGHIIDVKTGIDGIYDLLVEDDRIADISKDMDTEGCEI
                     INAGGKYVLPGLVDAHCHLRDPGFEYKEDIESGTRSAARGGFTSVACMPNTNPVIDNE
                     SVVKYILNKAKREGYVNVYPIGAITKGLKGEELAEIGELKFAGAVAVSDDGNPVKSSS
                     LMKKAMQYASMFDIAIISHCEDTDMAEDGVMNEGYTSTMLGLKGIPAAAEETMVAREL
                     ILSEYLDIPIHIAHVSTALSVELIRNAKKRGVKVTCETCPHYFSLTEEACMGYDTNAK
                     VNPPLRTTKDVQAIIEGIADDTIDIIATDHAPHHIDEKRVEFSIAANGMVGFETALPL
                     AVTKLVRPGIITMGKLVEKMSLNPAKLLGISKGTLEPGSTADITIVDIDAKFTVDPEK
                     FESRSKNSPFGGMELYGLVWGTIVNGKPVVSENILL"
     gene            complement(8288..9226)
                     /locus_tag="PLG72_01355"
     CDS             complement(8288..9226)
                     /locus_tag="PLG72_01355"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOJ79483.1"
                     /translation="MVLKSKDLLGLKDLTAEEIEYILDTAKAMKYVITSNNKRTPHLQ
                     GKSIVTLFYENSTRTRLSFELASKYMSASSANINSSGSSVAKGESLIDTGKTIDMMGT
                     DVIVIRHPQSGAPHLLAKHVKASVINAGDGMNEHPTQALLDMFTIREKKGTLRGLKVA
                     IIGDIYHSRVARSNIWGLTKMGAEVSLSGPATLLPPKMEMMGVKIYSTVHEALINADV
                     IISLRMQLERQKKALFPSIREYSRFFGLDENRLRLAKKDVLIMHPGPINRGVELTSAV
                     ADHEHSAINEQVTNGVAVRMALLYLLTRRGINEAAD"
     gene            complement(9316..9870)
                     /gene="pyrR"
                     /locus_tag="PLG72_01360"
     CDS             complement(9316..9870)
                     /gene="pyrR"
                     /locus_tag="PLG72_01360"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOJ79484.1"
                     /translation="MIDKAEIMDESAMKRAITRIAHEIIERNKGVEDVRLIGIQRRGV
                     PLAKMIASKIREVEGKDVPVGILDITFYRDDLSMLSEHPVVNGTEINFPVTDKVIVLV
                     DDVIYTGRTARAAIDAVMDIGRPRIIQLAILIDRGHRELPIRADYVGKNVPTSRTEMI
                     NVKVVEFDGVNKVTLSDIEPGKKH"
     gene            complement(10255..11175)
                     /locus_tag="PLG72_01365"
     CDS             complement(10255..11175)
                     /locus_tag="PLG72_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ79485.1"
                     /translation="MRNETFAITSEDAGKRLDIWLTSKLGDYSRSYVERLVEQGNVTV
                     GGKTVKAGYKLKDGDIVQVSVPGPKPLEVEAEDIPLDIIHEDEDIIVINKPRGMVVHP
                     AAGNYSGTLVNALLKHCSGSLSDINGVIRPGIVHRIDKDTSGVLVIAKNNNAHSVLSH
                     MLKEHDINRTYIAVAEGIVPADRGKIDAPIGRHPVDRKRMAVNLKSGRRAVTHFRVLE
                     RFKSATLLELRLETGRTHQIRVHMSYIGHPLVGDTVYGRKKQKYGFEGQALHAAVLGF
                     VHPGTGEYVEYSAEPPQEFMDLVEKLRQEK"
     gene            complement(11165..11737)
                     /gene="lspA"
                     /locus_tag="PLG72_01370"
     CDS             complement(11165..11737)
                     /gene="lspA"
                     /locus_tag="PLG72_01370"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOJ79486.1"
                     /translation="MILSVGINLCHDGLMNPYRKIQEDTEGVHEMLWIIITILVIAID
                     QITKYIVVRNIEPTEMIPVIDRFFYLTLHRNPGGAWGIFQNSGVMFLVLIPVISAFLV
                     YTIIRNNDRSLRAILGLILGGAIGNYIDRVAEGKVIDFLLFYIGPYPFPVFNAADIAV
                     TCGTVMLAVYILFIYKEPKTANQEEEKNEE"
     gene            complement(11703..13535)
                     /locus_tag="PLG72_01375"
     CDS             complement(11703..13535)
                     /locus_tag="PLG72_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ79487.1"
                     /translation="MKRISIIIFAVIFSVILSACQTGHELEQLQRLHERYESDMSGHK
                     YVNDDDILDKGPEKGGTLNLFTTKPDTLNPLLTSNSYTADFLGFVYESLTRLDQSQKA
                     VPLLSDRWSVSDDGLIWEFHIRDGILWHDGEPFTAYDAEFTIQTILNPSINSPYKSLL
                     ANVSQCVAVDSSTIRIALKKPNSFMPEMMTFPVIPKHQFAAVDVLTASKDFKPVGTGP
                     YRFASYDGGSIMLVLNDDWWYPDVDETLVSGGMYIEKIQVNVFNRIEDAMGAFQSGEV
                     DVVGLGFNEYLKYIDRTDLTIKSYASRNFEFISLNLEDPVLSDIYARRAIYLSIDRGR
                     IINELLRGAAEEADLPVLPTCWLEDAKETEKGNIPDKVEENALRPPDGPETDTAGGDN
                     TGADSSAQDESKNVTDPSTAKTADEVLTLGGWKKNEQGYYKVIKGSRRYLSLELLVNS
                     NNSMRVKAAQMICSQLEEAGIKTTLVQLPWKDMLNRVNNGRYKMAFLGCRVPQIPDIS
                     FMYSDSYLKYASSSGYSEAYNVSGYGSPAADEYISELFMENDAGSRKAVYKALKGQVL
                     ADCPYIGLYFLKDAVVYGRNVKGPMNPNTWNRFNDFIRWYKPVP"
     gene            complement(13546..14385)
                     /gene="pgeF"
                     /locus_tag="PLG72_01380"
     CDS             complement(13546..14385)
                     /gene="pgeF"
                     /locus_tag="PLG72_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOJ79488.1"
                     /translation="MKEYEKIGTGTVLVTDGHLKYIEFESLNKYKDMLTHFMSTRHGG
                     VSSGECSTLNLGFGRNDDRDNVIENFRIICNATAVGMESLVLTNQVHGTKVCRVDEGD
                     IGKGLMKESDLKGTDGLITTAPGVTMVTFHADCSPVFLFEPSVKAAALLHSGWKGTLA
                     DIVTNAISEMKKIPGFDAGRVEAVIGPSIGFCCFEVDEDVYMLFREKYDNDSFYCRVS
                     DRKWKIDLKGIIKARMAEAGVDTGKIHDSGICTKCRSDLFFSYRGDMGRTGSMAAFMQ
                     IRQ"
     gene            complement(14531..14794)
                     /locus_tag="PLG72_01385"
     CDS             complement(14531..14794)
                     /locus_tag="PLG72_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOJ79489.1"
                     /translation="MDVLKISAKSNPNSVAGALAGVIRERGVAEMQAIGAGALNQAVK
                     AVAIARGFVAPSGIELICIPAFTDIVIDGEERTAIKLIVEPRK"
     gene            complement(15004..15777)
                     /locus_tag="PLG72_01390"
     CDS             complement(15004..15777)
                     /locus_tag="PLG72_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOJ79490.1"
                     /translation="MKVLFIGDIVGGPGRKAVKQYIGELRSQYRFDYCVANAENAAGG
                     SGITYVTAQELYSCGIHAVTMGNHTWSKKEITTFIDSDTRIARPANYPAELPGKGTVI
                     IDGRLGVISLLGRIYMEPVDCPFKTLDMELEMLKRQVKAVIVDFHAEATSEKCALAWH
                     ADGRVSAVIGTHTHVQTADERILPCGTAFISDAGMTGPYEGVIGVKKELVIEKFRTSM
                     PVRFEVAKGPVQFNAVLLDIDEDTGRATGIRRISNVFSV"
     gene            complement(15872..17440)
                     /gene="rny"
                     /locus_tag="PLG72_01395"
     CDS             complement(15872..17440)
                     /gene="rny"
                     /locus_tag="PLG72_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ79491.1"
                     /translation="MCAIFDLAIGILIGLAIGAVIASFISFRIGYNRRKIQAEAEIGS
                     AEQESRRIINEALKTAESKKREALLEAKEEVHRSRIELDREIKERRNEIQRLERRLVQ
                     KEEALDKKVESLEQKDELLNRKLKEIQATQEKTAELHRKQLEELERISGLTVEEAKQY
                     LLRNVEDEVKHEMAVLVKDLEAKAREEADIKAREIIATAIQKCAADHVSETTVSVVPL
                     PNDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREVARITLEKLI
                     LDGRIHPARIEEMVEKAKKEVDNTIRQEGDRATFETGVHGLHPELVKLLGKLKFRTSY
                     GQNVLNHSIEVATLAGLMAAELGADVNLAKRAGLLHDIGKAVDHEMEGSHITIGAELA
                     KKYKEGNEVVNAIMAHHGDVEPTSIIAVLVQAADSISAARPGARRETLESYIKRIEKL
                     EEIANSFPGVDKCYAIQAGREIRIIVKPEDVSDDEIVLKAREIVKKIESELEYPGQIK
                     VNVIRETRAIEYAK"
     gene            complement(17767..19503)
                     /locus_tag="PLG72_01400"
     CDS             complement(17767..19503)
                     /locus_tag="PLG72_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ79492.1"
                     /translation="MTIVNGFIIFLMLLLLVYSLYRAMKRKSLLMLIPVCLQVFAAAI
                     SIMSFIYDVDVLMQVEAVYILFGIIPPSVLLIHDYRKMIKNFRAKGVYEGLVRSVAPD
                     KPFSSFPAEGINCIKKKKPLSEIIDDLGFLSDDIRTNFRKCLVHANTLLNKGENESAM
                     NVYWTLAKAAGNSFALFYNFGCLCYEQGFYGEAHNAFQRSLQLFSGADDERCTIYYNL
                     GNTSFMLGKYEKAAGFYEKSLEIRPDDTETLENLSYTYVMMGQADKGTEVMKKIPADD
                     SSYRPHYVWGRLYSEAGRLEEAEEELRKACRLKADSKEAREELAKVLMKQNKTDEAIT
                     ILDEILMLDPVNHPAWYNKANALAKKGLWKDAAAVYNEAVKLRPDFHKGWYNMALALD
                     ESGDRQAAIKAYMKTIELCPDFAEAYNNLGIALSLEGRREEALDIYEEGIKRDPGNYS
                     LFFNMGICLMEEKRYMDAAAAFRNALDLNPEELEIYYYLGAVLTEMRHYNDAIDAYST
                     ALKIKPADGELQYNLAAVYAMLGRYDIALENLKAAVEADRSLIEDIRNNSAFDGMRGR
                     TEFKKLLSPASA"
     gene            complement(19519..21963)
                     /locus_tag="PLG72_01405"
     CDS             complement(19519..21963)
                     /locus_tag="PLG72_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOJ79493.1"
                     /translation="MKSKKVQKKKRRASKQVQVAYFNEIQGILVLAFGVLMLLSFYIK
                     NSIGQFGMFLREASLGILGIPAFLIPPLVILYGALLIVAYDRLDIRRKPLYVLILFAL
                     VSALIQTGIYKPENYTGLTPIKALERFYTDGTALSGGGVIGGLISLPFLVTFQVPGTI
                     IILTALSLIDIILLTNKSIADFIRNIRGLFVRKKKPKRVDEEDMDDTQPVIEEDGDEI
                     TLRFDKPKVIDFRIEKTARELKKQQRTRGGAAKNDPVPEDEDETEIGIESGTDEDFAG
                     PEISPVELVAEDIRKSREKKKPARKSADDGSEEDRGPGLQEIVIKGPSTAKKQNTKYT
                     YPPYDLLIEEPSDSGNSRAHRNQALEGAKKLEETLYSFGVSAKVINVTRGPTVTRYEL
                     QPSAGVKVSRIVNLSDDIALNLAAPGIRIEAPIPGKAAIGIEVPNKEINMVRLREVLE
                     SNEFVQHTSKLAFAVGKDISGEPVVADIAKMPHLLIAGATGSGKSVCINCLIVSILYK
                     ASPDEVRLLLIDPKVVELNVYNGIPHLLIPVVTDPKKAAGALNWAVQEMVNRYKLFAD
                     KNVRDIRGYNELVSRTGDQPLMPQIVIIIDELADLMMVAPNEVEDAICRLAQMARAAG
                     MHLVLATQRPSVDVITGVIKANIPSRISFAVSSQVDSRTILDMAGAEKLLGRGDMLLH
                     PVGKPKPIRIQGANITDAEVERVVEFVKAQGDTRYDDEIIEEINNDGATDEGDSGGDN
                     DELLPQAIELVIDAGQASVSFIQRKFRVGYARAARIIDQMEARGIISGYEGSKPRQVL
                     ITRQQWHEMQMAQKDE"
     gene            22348..>22608
                     /locus_tag="PLG72_01410"
     CDS             22348..>22608
                     /locus_tag="PLG72_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79494.1"
                     /translation="MKILSSIAESIKSVPGFLKNIPKFFKDIRKIRTKLILVFMIPIL
                     LIAVQGIMTYTNSSRMARENMTESFVSSMENSGKYLEVILQTM"
BASE COUNT         4955 a         6194 c         4782 g         6677 t
ORIGIN      
        1 accaaaacgg ctcgttttcc gggcacaccc tcaacgcgtt ggtttcaaac agccagttta
       61 tcagtttctc atttgacatt tcaggatcct ccgatatatg gattataaat caaccgtttt
      121 tcaatcagga taatgacgcc gacaagtcat tccgtggaac ctgttgaatg accatctcgg
      181 ctttgccccc ggtccttgcg ctccttgctt tttcttagat attcctccca ccagtcctgg
      241 ttgactatcg tcccaaggtc tttgccaaga tggcttgaat cgccttcggt cactatcgta
      301 aaaaccccgt cttcatagtc gatgacagtt atcgccgtat tgtcgcacca ctccacgttc
      361 accatctctt ccagtgtgca gcttctgaaa tggcatagca tcgcccggat cgccgtgcca
      421 tgtgtcacga tgcatatgtt ttttccttcg ttcgctttga tgatagacat tacttcgccc
      481 aaaagacgtt cctggaactc ttccattgac tcaccgttgg gcattttgag cttgtgaggc
      541 cggttttccc atgcttcgta ctcatcgggc catcgctgtt caagctcgct ccacgccaca
      601 tcttcccagt ctcctccgtt tatttccctc aggttctcag atgttatgac cgaaagaccc
      661 ttgatatcag caatgtatct ggctgtctgg acagccctct ggaggctgct cgaacagatg
      721 acgtcgatgt cgacggcctt cagcctttcg gccactctct cggcctgaag atggcccttt
      781 tcggtaaggc cggaatttgt ccatccgtgg aaccgcctga ttttgttgcc taaagcttca
      841 gcatgcctga cgaatatcag ccgggtcttc aaagtatcac ctcgcagttc aggcattcag
      901 taccagtttg ccggtgatat cggcaacact gttgaaaccg tgcctcctca tatattgttc
      961 tattccttca cataccttta tgcaggcatc aggataaacc aggttcgcag tccccaccat
     1021 aactgcggaa gcgcctgcga gcatgaactc gaccgcatcg tttccgttga agatgcctcc
     1081 catcccaatg accggtatct taacggtttt ataaacctca tgcaccatcc tgactgctat
     1141 cggcttaact gccggtcctg aaagcccgcc ggtgttgttg gcgagtacag gtcttctcgt
     1201 ttcgatatct accgccatgc caagcaacgt atttatcagt gagaccgcat ctgcacccgc
     1261 ggcctccgct gctgctgcga taccggatat gctggtgacg ttcggcgtca gtttcacgat
     1321 aagcggtttc cggcaatgtt tcctcacctg tgaagttatc tcgtatatac cctcgggtgt
     1381 attcccgaaa gcgacacaac ccgctttcac attgggacat gacacgttta gctcgatcgc
     1441 atcgatgtcc gcatccgata gtatctccga catctcgcag tattcttcaa tagtattgcc
     1501 tgctatattt gctatgatgg ctgccgaata ctgccggagg aaccttatct cattttcaat
     1561 gaaatacctg acgcccgggt tctgcaggcc aacgctgttc agcattcctg cgggcgtttc
     1621 cgcgaccctt gggggtttat ttccttccct cggtcttagt gtcagtccct tcacacttat
     1681 gccgccgatt ttgttcaggt ccgcatagtc tgcatgctca cgtccgaaac cgaacgtgcc
     1741 cgatgccgct atgacaggat tcttcagcct tataccgcat atatcgactg aaaggtctat
     1801 atcgctcatc atatcctccg ttcctatact gttatcatat caaaaacccg tatctattgc
     1861 atacgggtct tattcaaata tcacatcctc gctgtcgaac acaggaccgt ccttgcaaac
     1921 acggacatac tgccagcctc cgccgtccgt agttactttg catgcgcatg ccaggcatgc
     1981 gccgaatccg catcccattc tctgttccat ggatatctgg cacctgacac gatattcgcc
     2041 cgcgatggca gccactgtcc tgagcattgg atttggtccg catgcatata tcatatcgaa
     2101 ttctcccggt aaaatgtctc tttcgagtat atccgtaaca aatcccttta ttccgcaact
     2161 cccgtcttca gtggctgttt cgagtgaact ggatctcttt ctgaactcgt cgagaagcat
     2221 tatgttttct gaagatcgaa aacccaggta tgaccttttc acaattgctt tgctctcatc
     2281 aagcaggaaa agcagcggga atatcccgat acctccgccc actacggcta tccggcgata
     2341 ggagatattc aggtcgaagc ccctgccgag cggaccgaga atatccaggg aatcaccgat
     2401 ttttttccct gccagtattt ccgtgcctga accctttacc tggaaaaata tatcgaattc
     2461 acctttttcc ctgtctaccg agcatatgct gaccgggcgc ctcagcagcg cctgtgttcc
     2521 ttcgcagcat tttatgttga caaactgtcc cgggacggca ttggctgaaa cgtattcaga
     2581 agccaccgtc atgcggtaaa tgttttctgc cgctttctcc acattggtaa tcgtttctct
     2641 gagcaacctt gacatctgaa tcctccctgg cttttgtact tttttgagca ccggtcatat
     2701 aacggtctga gtccggtcag ctgaacccac taaggtcgat aacatccgga cttattccct
     2761 gctgtttcat gcggatgcag ctaagcacag cccttgcagt atccagtgac gtcaggcacg
     2821 gtatcgacat ctccacggct ttccgccgta tcttgaatcc atcgcgttcc ggctgtcttc
     2881 cccttgtagg agtatttatc acaaggttta tctttcctga tgctataaga tccagcagat
     2941 cgggagagcc ttcccctatc ttctttactg catttgtcgc aataccatgc atattgagca
     3001 tccttgcagt gtttcctgtt gcccagagtg tgaacccaag cttttcgaac tgttccgcga
     3061 ttccgggcag ttcagtcttg tccgaatccc tgactgtcat caggatgcct ccccccggcg
     3121 aaggcagctt catacctgta gccatgatgc ccttataaag ggcttcgtgg aaattacgtc
     3181 ctatgccaag cacttctccc gttgatttca tttcaggacc caggctcacg tccacatcat
     3241 ggagtttctc gaaggagaac actggcactt ttatggcaac atgatccatg gtcctgtaca
     3301 gtcctgtccc gtatccgaaa tctttcagtt tcctgccggt catcaccttc gtcgccacat
     3361 tcaccatcgg tatgccggtg accttgctga tgtaaggcac agtcctgctc gaccttggat
     3421 ttacttcaat aacatatatt tcatcattgt acagcacata ctggatattg atcatgccga
     3481 ctacattcag cgcttttgcc agcttctccg tataatccac gatcttgtcg atcacagggc
     3541 gtcccagcga aagagccgga tatacggaaa tgctgtcgcc cgagtgtacg cctgcacgtt
     3601 ccaggtgctc cattatacct gggataagta tgtcttcgcc gtcggaaatg gcatcgactt
     3661 ctatctcttt gcccatcagg tacttgtcga tcaggatggg gtgctcctgc ttaaccctgt
     3721 tgatgatctc cataaactcg atgatgtcat tgtcgttata tgctatctcc atcccctgtc
     3781 cgccgaggac gtaagacgga cggaccagca ccgggtagat gagctcattc gcggcagcca
     3841 gcgcttcttc aagagtaaag actgtctttc ctctcggtct cggtatgccg agtttttcaa
     3901 gaatcttgtc gaatctttcc ctgtcctcgg ccgcgtccac atcttcagcc tgtgtaccga
     3961 atatccttac gccaagtctg ctcagcttat tgacaagctt gatggctgtc tgaccaccga
     4021 actgtacgat cactccttct ggtttttcat gttcgattat gttcatgacg tcttcaatgg
     4081 tcagcggttc gaaatacagc ctgtccgcgg tatcgaaatc cgtgctgacc gtttccgggt
     4141 tgttgtttgc tatgatcgtc tcatatccct gctctttcag cgcccatacc gaatgcaccg
     4201 agcaatagtc gaactctatg ccctggccta tccggatagg ccccgaacct atgacaagta
     4261 ttttcttatt tccggaactt gctgttttgc cgcattgacg ataagtggag taatagtagg
     4321 gcgataccgt atcgaaacgt tcagtgcagg tatcaacaga tttgaaggat gcatttatcc
     4381 cgttttcatt tcttagcctt gtgatgtctt caagactgca gcctgcataa ccggctattg
     4441 tttcgtccgt gaagcccatc tccttcgccc tgcgtacgat atcagcatca agagttgctt
     4501 ttgtccgtgt ttttagggat tcctccatcc tgacgatgtt cgatatttta tccaggaacc
     4561 attgatcgat tttggttttt tcatatataa gttcggccga aattcctctt ctcagcgctt
     4621 ctgctattat gaacagcctt tcgtcatctg cgttagcaag tttatcttcg atctcttcgg
     4681 ttcgatattt cacgaatatc ttctgctcca acgtagatat cccaagttca agcgacctta
     4741 cagccttcat cagggctccc tcaaatgagt cgcctatggc catgacctca ccggtcgcct
     4801 tcatctgtgt gccaagtgtc ctgtctgccc ttacaaattt atcaaagggc catttgggta
     4861 tcttcagcac cacgtaatcc aggacaggct caaaggactt gagagtcttt cctgttgttc
     4921 tgtttcctat ctcatcgagc gtatatccta ttgcgatctt tgttgcgacc cttgcgatgg
     4981 gatatccgga cgctttcgaa gcaagggccg atgagcggct cactctcgga ttgacttcga
     5041 tcaccgcgta ttccttgcct gttggatgca acgcaaactg aacattgcat cctccttcga
     5101 tctcaagtgt atttatgatc tttatcgctg catcatgcag catcctgtat tcttcttccg
     5161 aaagcgtctg gcagggcgca accactatac tgtcgcctgt atgcacaccg accgggtcca
     5221 cgttctccat accgcagatg attgctgcat taccttttgc gtctctcatt acttcgaatt
     5281 ctatttcctt ccagccggaa atacatcttt ctatcaatac ctggccgaca cggctgagcc
     5341 tcaggccatt cgaggcgatc gcggcaagct gatcgctgtc atcggcaata ccgcccccgg
     5401 ttccaccaag ggtatatgca ggcctcacta tgaccggata tccgatctca tccgcaaagt
     5461 ccagcgcgtc ttccgttgac gtcacgactt tgcttgcaac gcacggttct ccgatagatt
     5521 ccatcgcatc cttgaatgcc tgcctgtctt cagccatttt tatcgctttc gtcgcagttc
     5581 cgagcagctt tactcccatt ttttccagaa aaccggattc tgaaagttcc atggccagat
     5641 tcaggcctgt ctggcctccc agtgtcggaa gcatactgtc tggtctttcc tttgtgatga
     5701 tcttcttcag cgtttcagca tccaggggct ctatatatac tttatccgcc atgttcgtat
     5761 cggtcattat agttgccgga ttgctgttca caagtacgac ttccaggcct tcttccctca
     5821 gggcccggca tgcctgggtg cctgcatagt cgaattcggc agcctggccg atgactatcg
     5881 gtccagaacc tattaccagg actttgttga tatcattacg tcgtgacatg cttatctcct
     5941 ttcatccgtc tttgaatatc tgtcggtctg aacttcatgt accggtctcc cgtcatcgtg
     6001 taacaggaag gttccaccga ttccgcgtcc ctgatacttc cttgtttgtt ttatcgctgt
     6061 tgaccttttc tttcttcaag tgccctgcgg atgtcttccc gcatcctgac ggcctcttcc
     6121 ctggccgcat gatcgaatcc gctttctccg tatttctcca gccatgcttc agatttccat
     6181 gcgcacatga tgctcctgga cgcattcact atggcgccaa gtccgtcgtc gttgaaggcg
     6241 caggctgcgt cgcgggctgt gccgccctgg gcgccgtatc ctggtacgag gaagtatgac
     6301 cttttcatta tctttctcag gacacaagcc tgttcgggat acgtggcgcc aaccacggct
     6361 cctatgctgc tgtagccgtt ctcacccatg gctgccatac tccattcgtc gaccagtcct
     6421 gcaacttttt cgtaaacgtg ttttccgtcc atggttataa ggtcctgtat ctgtccggac
     6481 gatttatttg acgttttcac aagcacgaat atgcctttgc ctgaagcggc gcattcatcg
     6541 atgaacggct gaacgccgtc gatgccgagg taagggttga cggtcagcgc gtccgtatcg
     6601 aaagcagaca gcgcagcatc atctatttcc gtctttccaa gatatgcgtc cgaatatgcc
     6661 cgggctgtcg agcctatatc gttgcgtttt ccgtcagcga tgacaatcat gccccgcgtc
     6721 ctggcgtacc tgcaggtctc ggcgaacgcc ctgatcccgt cgacgccgta catttcataa
     6781 taggcaagct gcggcttcac agcgggcacg atatcgcaga gggcatctat cagcctttta
     6841 ttgaactcca ggatcgcctc cgctgcgccg gcgaatcctt tcccatgttc gctgaaagcc
     6901 ttttccctga tacatgacgg tatatactcc agtttcggat caaggcctgc cacggccggg
     6961 ttcccatatt tccttatggc tgcgatcagt ctgtcgataa acattcctgc aactcctcta
     7021 cagcaaaata ttctcgctaa cgaccggttt gccgttcacg atcgtgcccc agaccagtcc
     7081 gtaaagctcc atgcctccga atggtgaatt ttttgacctg gattcgaact tttccggatc
     7141 gaccgtaaat tttgcatcta tatctacgat cgttatgtcg gcagtacttc ccggctccag
     7201 tgtgcctttg cttatcccca gaagctttgc cgggttcagg ctcatttttt caaccagctt
     7261 tcccatcgtg atgataccgg gtcttaccag tttagtgacg gcaaggggca gtgcggtctc
     7321 gaaacccacc atgccgtttg cggcaatgct gaactcaact cttttttcat cgatatggtg
     7381 aggtgcatgg tctgttgcta tgatgtcgat cgtgtcatca gcaatgcctt ctatgatggc
     7441 ctgcacatcc ttggtggtcc tgagcggcgg gttgaccttt gcattggtgt cataacccat
     7501 gcaggcctct tcagtcagtg agaaataatg cggacatgtt tcacatgtca cctttacacc
     7561 gcgtttcttt gcattcctga tgagttccac cgacagtgcg gtactgacgt gtgctatatg
     7621 tatcggtata tccaggtatt cggaaaggat caattcacgg gctaccatgg tctcttcagc
     7681 ggcagcgggt atgcctttca gccccagcat tgtcgatgtg tagccttcgt tcatcacccc
     7741 gtcttcagcc atatctgtgt cttcacagtg ggatatgatc gcgatatcga acatggaagc
     7801 atactgcatc gcctttttca tcagggagga acttttcacc gggtttccgt catcagacac
     7861 ggcaacggcg ccggcaaact tcaactctcc gatctctgca agttcttcgc ctttcagtcc
     7921 ctttgtaatg gctccgatcg gatagacgtt tacataccct tctctttttg ctttgttgag
     7981 gatgtatttg acgacggatt cattatcgat gaccggatta gtatttggca tacaggcgac
     8041 tgaagtgaag cctcccctgg cagcactcct tgtaccgctc tctatatcct ccttgtattc
     8101 gaatccgggg tccctgagat ggcagtgggc atccacaagg ccgggaagta catacttgcc
     8161 cccggcattg attatttcac atccttctgt atccatatcc tttgaaatat ctgctatcct
     8221 gtcatcttcg acgagcagat cgtaaatgcc gtcgatcccg gttttcacat cgattatatg
     8281 cccgttttta atcagcagct tcattgatcc ccctcctggt cagaagataa agcaatgcca
     8341 tccttacagc aacaccgttg gtgacctgct catttatcgc ggaatgctca tggtccgcaa
     8401 cggcactggt cagttcgacg ccacgattta tgggtcccgg atgcatgatg agtacatctt
     8461 ttttggccag gcgcagcctg ttttcatcca gcccgaagaa cctggaatac tcccttatcg
     8521 aagggaacag tgcctttttc tgccgttcaa gctgcattct cagcgaaatt atcacatcgg
     8581 cgtttatcag tgcttcatgc acggtactgt agatctttac gcccatcatt tccattttcg
     8641 ggggcagcaa tgtagccgga ccggaaagcg acacttctgc gcccattttg gtcaatcccc
     8701 agatgttgct ccttgccacc ctgctgtggt agatgtcgcc gattatggca accttgagcc
     8761 ccctgagggt acctttcttc tccctgattg tgaacatgtc cagcagagcc tgcgttggat
     8821 gttcattcat cccgtctcca gcattgatca ctgatgcttt cacatgcttt gccaaaagat
     8881 gcggagcgcc tgactgcgga tgccttatga cgattacgtc cgtccccatc atatcgattg
     8941 tttttccggt gtctataaga gattcaccct tggctacact gcttccggaa gagttgatgt
     9001 ttgcggaact tgcgctcata tattttgatg caagctcgaa tgaaagccga gttctggtac
     9061 tgttttcata aaacagcgtc actatggatt tgccctggag atgaggcgtc cttttattgt
     9121 tggatgtaat aacgtatttc attgcctttg cagtatcaag gatgtattcg atttcctcag
     9181 ccgtcagatc tttaagccca agcagatctt tggatttcag aaccatatgt tttaccgcct
     9241 tttatcagat ttgaccagcc ggagagcatg ttgatgctcc cgtattctaa aaaagagtaa
     9301 ggaaactatc cctactcaat gctttttgcc aggttcgata tcgctcagtg tcactttgtt
     9361 gactccgtcg aattccacga ctttaacatt tatcatctcc gttcttgacg tcggaacatt
     9421 cttgcccaca taatcagcac gtatcggcag ttccctgtgg cccctgtcga tcagtatggc
     9481 aagctgtatt atcctcggcc tccctatgtc catcaccgcg tctattgccg cacgggccgt
     9541 cctgccggta tatatgacgt catccacgag cactatgacc ttgtccgtta caggaaaatt
     9601 gatttccgta ccgttgacga ccgggtgctc cgacagcatg ctcaggtcgt ccctgtaaaa
     9661 agtgatgtca agtatgccta ccggcacatc cttgccttcc acttcccgga tctttgacgc
     9721 gatcatcttt gccaggggca ctccgcgcct ttggatgcct atgaggcgca cgtcttcaac
     9781 gcctttgttc ctctcgatga tttcgtgcgc gatcctggta attgccctct tcatggcgct
     9841 ttcatccatg atttcagctt tgtcgatcat attaaggcca cctcccgttt cctgcaattt
     9901 tcttaaatat actaaaaaag cttcctgtca gcggcaggaa gcaataatat cacaggcaca
     9961 gcctgtaaac aacagaccca tatgataccc ttccagcctc acaggactaa ttaaaaggtt
    10021 tttccaaatt ccagtttata ttaacatata aaattacatg ttgtctatat ctttttttag
    10081 aatattatac gttttcgata tattaaaagt ggatccgttc tcaggatatg atccgaatca
    10141 tgggataacc ggttatttca acttgatttt tgagtccaga tgaagtcatg gtatgactta
    10201 tctaagtcca gactatttga ttcggcaaat tgaataacct ttacgtctta atatttactt
    10261 ctcctggcgc agtttctcga ccaggtccat gaactcctgc ggcggctctg ccgaatactc
    10321 cacatattcg cctgtacccg ggtgtacgaa cccaagcacg gccgcatgga gcgcctggcc
    10381 ttcaaagccg tatttctgct ttttcctgcc gtataccgtg tccccgacca gtggatggcc
    10441 tatatacgac atatggaccc ttatctgatg agtccggccg gtctccagtc tcaattccag
    10501 taaagtggca gatttgaagc gttccagcac cctgaagtgt gtcacggctc tcctgccgct
    10561 cttcaggttt acagccatcc tcttcctgtc cacaggatgc cttcctatag gcgcgtcgat
    10621 tttcccgcgg tcggcgggga cgatcccttc cgcaacagct atataggtcc tgttgatatc
    10681 atgttctttc agcatatgcg acaaaacaga atgggcattg ttattcttcg cgataaccag
    10741 cacacctgaa gtgtccttgt cgatcctgtg aacgatgccc ggcctgatca cgccgttgat
    10801 atccgaaagg gaaccgctgc aatgtttcag cagagcattg acaagagtgc cgctgtaatt
    10861 gcctgcggca ggatgcacca ccatgcccct tggtttgttg atgacgatta tatcttcatc
    10921 ttcatgtatg atatccagcg ggatatcttc agcttcgact tccagcggct tcggcccggg
    10981 tacgcttacc tgcacgatgt cgccgtcctt gagcttatag cctgctttga cagttttccc
    11041 gcctactgtt acgtttccct gctctacgag cctttccaca tatgacctgg agtaatctcc
    11101 aagtttgctt gtcagccaga tgtccaggcg tttgccggca tcctctgaag ttatggcaaa
    11161 ggtctcattc ctcatttttt tcctcttcct ggtttgctgt ttttggttct ttgtatataa
    11221 aaagtatata gacggccagc atcacagtac cgcatgtcac ggcaatatcg gcggcattga
    11281 ataccgggaa aggatatgga cctatgtaaa acagcagaaa atcgatcact ttcccttcag
    11341 ccacccggtc tatataattc cctatagctc ctcccagtat cagccccagg atcgccctga
    11401 gggacctgtc attgttcctg atgatcgtat agaccaggaa agccgagatc accggtatca
    11461 gtacaaggaa catcacgccg ctgttctgga atatccccca ggcgccgccc gggttcctgt
    11521 gaagcgtcag gtaaaaaaac ctgtcaatta ccggtatcat ctccgtgggt tcgatatttc
    11581 ttacaactat gtatttggtg atctgatcta tggcaattac cagtatcgtt atgattatcc
    11641 agagcatttc atggactcct tccgtgtctt cctgtatttt tctatacggg ttcatcaggc
    11701 cgtcatggca caggtttata ccaacggata aaatcattga acctgttcca tgtattcggg
    11761 ttcatcggcc ccttgacatt cctgccgtat acaaccgcat ccttcaggaa ataaagcccg
    11821 atatacgggc aatcggccag gacctggcct ttcagtgcct tatagaccgc tttccgtgat
    11881 cctgcatcgt tttccatgaa taattcagat atatattcgt ctgcggccgg gctgccgtaa
    11941 ccggatacat tataagcttc ggaatagccg gacgatgacg catatttcag gtaactgtcc
    12001 gaatacatga acgatatgtc cggtatctgc ggcaccctgc atccgagaaa agccattttg
    12061 tatctgccgt tattgaccct gttcagcatg tctttccatg gaagctgtac aagggtagtc
    12121 tttatgcctg cttcttctag ctggctgcaa atcatctgcg cagctttgac gcgcatgctg
    12181 ttgttggaat tgaccaggag ttccaggctg agatacctcc tgcttccttt gatgaccttg
    12241 taatacccct gctcgttttt cttccatcct cccagagtca gcacttcatc agccgtcttt
    12301 gcggtactgg gatcggttac gttcttgctt tcatcctgag cactggaatc agcaccagtg
    12361 ttgtctcctc ctgcagtgtc ggtctccgga ccgtccggcg gccgcaaggc attttcctct
    12421 accttgtcgg gaatattccc tttctctgtt tctttcgcgt cttcaagcca gcaagtggga
    12481 agtacaggca aatccgcttc ttcagcagct cccctcagca actcgtttat gatccttccc
    12541 ctgtcgatgg acagatatat agcacgcctt gcatatatat cggataatac gggatcttcc
    12601 aggttcagcg aaatgaattc gaaattcctg gacgcataac ttttgatggt gagatcggtc
    12661 ctgtctatat acttgaggta ctcattgaaa ccaaggccga ccacatccac ttcgccagac
    12721 tggaatgccc ccattgcatc ttcgattcta ttgaatacat tcacctggat tttttctata
    12781 tacatgcctc ccgataccag tgtctcatcg acatccggat accaccagtc atcattcagg
    12841 acgagcatga tactgcctcc gtcatatgat gcgaacctgt atggacctgt cccgaccggt
    12901 ttgaagtctt tcgacgctgt gagtacatcc accgccgcga actggtgctt tgggattaca
    12961 gggaaggtca tcatttcagg catgaatgaa ttaggcttct tcaatgctat cctgatggtt
    13021 gacgagtcga cggcaacaca ttgggacaca ttggcaagca gggacttata aggtgaattg
    13081 atcgacggat tcagtatcgt ctgtatcgtg aactccgcat catatgcagt aaaaggctct
    13141 ccgtcatgcc agaggatccc gtcgcgtatg tggaattccc atatcagtcc gtcatctgat
    13201 accgaccatc tgtctgaaag cagaggaact gctttctggc tttgatcaag acgtgtcagg
    13261 ctctcataca cgaaaccaag gaaatcagca gtatatgagt tggaagtcag caacggattc
    13321 agtgtatccg gttttgtcgt gaaaagatta agggtgcctc ccttttccgg acccttgtcc
    13381 agtatgtcat catcattgac atatttatga ccgctcatat cagactcgta tctctcatgg
    13441 agcctctgaa gctgctccag ttcatggcct gtctggcacg cggataatat gaccgaaaat
    13501 attaccgcaa aaatgattat gctgatcctt ttcatctata gctccttact gtcttatctg
    13561 cataaaagct gccatactgc ctgtcctccc catgtcgccc ctgtatgaaa agaacaggtc
    13621 gctgcggcat ttagtgcata tgccgctgtc gtgtattttc ccggtatcga cgcccgcttc
    13681 agccatcctt gctttaatta tccctttcag atcgatcttc catttcctgt ctgatacacg
    13741 gcagtagaac gagtcattat cgtatttttc cctgaatagc atgtaaacgt cttcgtccac
    13801 ttcaaaacag caaaatccga tggatggccc gataactgcc tcgacccttc ctgcatcgaa
    13861 accagggatt tttttcattt cacttatcgc attcgttact atatccgcaa gtgtcccttt
    13921 ccaccctgaa tgcagcaacg ccgcagcttt caccgaaggc tcgaaaagaa ataccggcga
    13981 acagtctgcg tggaacgtga ccatcgtgac tcccggagca gtcgtgataa ggccgtccgt
    14041 ccctttcagg tcgctttctt tcataagtcc ctttcctatg tccccttcgt cgacccggca
    14101 tacttttgtc ccatgcacct ggttagtcaa aacgaggctt tccattccga ctgcagtggc
    14161 gttgcaaatt atcctgaagt tttctatcac gttgtccctg tcatcattcc gcccaaatcc
    14221 taggttcagc gtgctgcatt ctccggagct cacaccgccg tgcctcgtgc tcatgaaatg
    14281 cgtaagcatg tccttgtatt tgttcaggct ttcaaactca atatatttca aatgtccgtc
    14341 agtgacaagg accgtacctg ttcctatctt ttcgtattcc ttcaattact tccctccgct
    14401 ctgaccacat tcacatatta tcatatccta tatccattct caatacggga caggttttgc
    14461 acaggccaaa gccgggacag aaaaaaactg ccagtcatga cataaccggc agcatacatg
    14521 ggatattttt ttattttctt ggctctacta taagttttat agccgttctc tcctcgccat
    14581 cgataacgat atccgtgaaa gccgggatac agatcagttc aattccggac ggcgccacga
    14641 acccacgggc aatagcaact gcctttactg cctggttcaa agcaccggcg cctatagcct
    14701 gcatttctgc cacgcctctt tctctaataa cgcctgccag tgcccctgct acggagtttg
    14761 gatttgactt tgctgagatt tttaagacat ccatgataat aaccccctat tgcatgccat
    14821 attatatttt atattattat agaaaaattc tacattaaaa cccaaattcc tctttaatag
    14881 tctaaaattt agtaaaataa aatcaattta tacgtttatt cattgtattg cgtatacaat
    14941 cgtatatttg cattttccgg cctggaacag tcacgccctg taagtatagc aaaatatgat
    15001 atgtcatacg ctgaacacat ttgatatacg gcggatccca gtagccctcc ctgtatcctc
    15061 atcgatatcc aggagcactg cattgaactg cacagggcct tttgccactt cgaaacgtac
    15121 aggcatcgaa gtgcggaatt tttcgatcac aagttccttt tttactccga tcacgccttc
    15181 ataaggccct gtcatgcctg cgtcgcttat gaaagccgtt ccgcatggca atatcctctc
    15241 gtctgcagtc tggacatggg tatgggtgcc gatcacggcg ctgactctgc cgtcagcatg
    15301 ccaagctagc gcgcatttct cggaagtagc ctcagcatgg aaatccacga ttactgcctt
    15361 cacctgtctt ttcagcatct caagttccat gtccagagtt ttgaacgggc aatcgacagg
    15421 ttccatgtag atccttccaa gaaggcttat aacaccaagc cttccgtcta ttatcacagt
    15481 gcctttcccg ggtagttccg caggatagtt ggcaggcctg gcgatccttg tgtcactgtc
    15541 gatgaaagtg gtgatttctt tcttggacca tgtgtggttc cccattgtga cagcatgtat
    15601 tccgcagctg taaagttcct gtgccgtcac atatgtaatt ccggaaccgc cggctgcgtt
    15661 ttcggcattt gcgacgcagt aatcgaacct gtactggctg cgcagttctc cgatatattg
    15721 ttttaccgct ttccttccag ggccgcctac tatatctcct ataaacaata ccttcaaatc
    15781 tcttatcctc cgtcaataat aatatgtgcg tgatttattt tactataaca taaaaagcgt
    15841 gcaatgcacg ctttttattt cgattccgat ttcattttgc atattcgatc gctcttgttt
    15901 cacgaatgac attgaccttt atctgtccgg gatattcgag ttcgctttcg atcttcttta
    15961 ctatttccct tgctttcaat actatttcat cgtcagaaac atcttccggc ttgactatta
    16021 tccttatttc cctgcctgcc tgtatggcat agcacttgtc aacaccgggg aaggaattcg
    16081 ctatctcctc cagtttctcg atccgtttga tatacgattc aagcgtctct cttcttgctc
    16141 ccggtcttgc tgcggaaatc gaatcggctg cctgtacaag tacggctatg atcgatgtag
    16201 gttccacgtc tccgtgatgt gccattatgg cattcactac ttcgtttcct tccttgtact
    16261 tcttggcaag ttctgcgcca atcgtgatat gagaaccttc catttcatgg tcgacagctt
    16321 tgcctatgtc atgcagcagc ccggctcttt tcgccagatt cacatctgct cccagctcgg
    16381 ctgccatcag gcctgcgagg gtcgccacct cgatggagtg gttcaatacg ttctgaccgt
    16441 aacttgtcct gaacttgagc ttcccgagaa gtttgaccag ttcgggatgc aatccgtgca
    16501 ctccggtctc gaatgtggcc ctgtcgcctt cctggcgtat cgtgttgtcg acttctttct
    16561 tggccttttc taccatttct tcgatcctgg cgggatggat ccttccatca agaataagct
    16621 tttcaagggt aatccttgcc acttctcttc ttatgggatc aaaacctgag agaataactg
    16681 cttccggagt atcatcgatg ataaggtcta ttccggtaag agtttcgaga gtcctgatgt
    16741 tccttccttc gcggcctata atgcggcctt tcatctcatc attgggcagt ggtacgacag
    16801 aaactgttgt ctccgataca tggtcggcag cacatttctg tatcgctgtg gcaataatct
    16861 ctcttgcctt tatgtctgcc tcttccctgg cctttgcttc cagatccttc acaagtactg
    16921 ccatttcgtg cttcacttca tcttcaacgt tcctgaggag atactgtttc gcttcttcaa
    16981 cggtcagtcc ggatatcctc tccagttcct caagctgctt cctgtgaagt tccgctgtct
    17041 tttcctgggt ggcctgtatc tctttcagct ttctgttcag caactcatcc ttctgctcaa
    17101 gtgattccac ttttttgtcc agagcttctt ctttctggac gagccgcctt tccagcctct
    17161 ggatctcgtt ccttctctcc ttgatttccc tgtcgagttc gatcctgctt ctgtggacct
    17221 cttccttagc ttcgagcaat gcttcccgct ttttgctttc ggctgttttc aaagcttcat
    17281 tgatgatccg cctggattcc tgctcagcgc ttccgatttc agcttcagcc tggatctttc
    17341 tgcggttata accgatccga aatgatataa aagaagcaat tactgctccg attgcaaggc
    17401 caatcaaaat cccaatagcc aaatcaaaaa tagcacacac ctcctgttat tcagttttca
    17461 atggtcagtc gccggtcccg ggaatgaacg gccggcttca atcaacagcc tgcccttctg
    17521 cacgggcagg cccgatttaa gccaacaacc ggtcctttcc tgtcagcacc ggctggtttc
    17581 aatgctcaaa cagcctgtaa tgtctgtttt cttacgaaac atagcttaat tgtaaacttt
    17641 tttaattttt atgtcaagaa aaacaaaact tccgtcacaa tgcacaaacg catgaccggc
    17701 ttgccagagc cgttccgtct cttataagag tctacccggc ctgatgcgtc cggaaacagt
    17761 aaaatttcag gcagaagctg gagataacag ctttttgaat tcagttctgc cgcgcatgcc
    17821 gtcaaaagcg ctgttgttcc ttatatcctc gataagggac ctgtcagctt caactgcggc
    17881 tttcaaattc tccagggcta tgtcatacct tccaagcatt gcatatacgg cagcaaggtt
    17941 atactgcagt tcaccgtcag ccggctttat tttcagagct gtactgtatg catcgatggc
    18001 gtcgttgtaa tggcgcatct ctgtcaacac cgcgccaaga taataatata tctcaagttc
    18061 ttcaggattc agatcaagag cattcctgaa agcggcagcc gcatccatgt accttttttc
    18121 ttccatcagg caaatgccca tattgaaaaa caggctgtaa ttgccgggat cccttttaat
    18181 accctcctca taaatgtcca gcgcttcttc ccgcctgccc tccaggctga gtgcgatccc
    18241 aaggttgtta tacgcttcgg caaagtcggg gcacaactca atggttttca tatatgcttt
    18301 tatagccgcc tgcctgtcac cggactcatc cagcgccagt gccatattgt accacccttt
    18361 gtggaaatcc ggtctgagct tcactgcttc attataaacg gccgcggcat ctttccagag
    18421 cccctttttc gccagcgcgt ttgctttgtt ataccaggcc ggatggttga caggatccag
    18481 catcaggatc tcgtctaata tggtaatggc ctcatctgtc ttattctgtt tcatcaatac
    18541 ctttgccagt tcttcgcggg cttctttact gtccgctttc agcctgcacg cttttctgag
    18601 ttcttcttca gcctcttcca gcctcccggc ctcagaatac agccttcccc atacgtaatg
    18661 cggccgatag gacgaatcgt cggcaggaat ctttttcatg acctccgtac ctttgtcagc
    18721 ctgacccatc atcacatagg tatatgacag gttctcgagc gtttcggtat catcaggcct
    18781 aatttcaagg gatttctcat aaaaacccgc agccttttcg tatttcccca gcataaatga
    18841 agtattccca agattatagt aaatagtaca cctctcatcg tccgcaccgc tgaaaagctg
    18901 gagggatctt tggaatgcat tgtgtgcctc cccgtaaaaa ccctgttcgt agcagaggca
    18961 tccgaaattg taaaacagcg cgaaagagtt gccggctgct ttcgctaaag tccagtaaac
    19021 attcatagcg ctttcatttt cgcccttgtt cagcaaagta ttggcatgta caagacattt
    19081 cctgaaatta gtcctgatat catcggacaa gaagcccagg tcatcgatta tctcggaaag
    19141 cggcttcttc tttttgatgc aattgatgcc ttccgccgga aaagatgaaa agggcttgtc
    19201 cggagcaacg ctcctgacaa gtccttcata tacgcccttc gctctgaagt ttttgatcat
    19261 tttcctgtaa tcatggatca gcagcactga cggagggatt attccgaaaa gtatgtaaac
    19321 cgcttctacc tgcatcagca catcaacatc gtatataaag ctcattatcg atatcgcagc
    19381 cgcaaagacc tgcaggcaga cgggaataag cataagaagg ctctttcttt tcattgccct
    19441 gtatagtgaa tagacaagca ataaaagcat caggaaaatg atgaacccgt tgacgatcgt
    19501 catgacgatc ccccttaatc attcatcttt ttgagccatc tgcatttcgt gccactgctg
    19561 tcttgttata agcacctgcc ttggtttgct gccttcatag ccgctgatga tgcccctggc
    19621 ttccatctgg tctatgatcc ttgccgctcg tgcatatccc accctgaact tgcgctgtat
    19681 aaaggatact gacgcctgtc ccgcatcgat cacgagctcg atagcctgag gcagcagttc
    19741 gtcattgtca cctccgctgt caccctcatc agttgctccg tcattattta tctcttcgat
    19801 aatttcgtca tcatacctgg tatcgccctg ggccttgacg aattcgacca ctctctccac
    19861 ttccgcgtcc gttatgtttg cgccctgtat cctgatcggt ttcggttttc caacgggatg
    19921 gagcagcatg tcgcctcttc cgagcagttt ttcggctccg gccatatcga gtatcgtcct
    19981 ggaatccacc tgtgaagaaa ccgcgaatga gatcctggat ggtatattcg ccttaatgac
    20041 tcccgtgatg acatccaccg aaggcctctg tgtcgcaagc accaggtgca tcccggccgc
    20101 tcttgccatc tgtgcaagcc ggcatatggc gtcttcgacc tcattgggcg cgaccatcat
    20161 caaatcggca agctcgtcta ttattataac tatctgcggc ataaggggct ggtcgcctgt
    20221 ccgggacacc agttcgttat aacctctgat atccctgaca ttcttatcag caaaaagctt
    20281 gtacctgttc accatctcct gtactgccca gttgagcgct cctgcagctt ttttggggtc
    20341 agtgacgaca gggatcagca gatgtggtat gccgttatac acgttaagtt caactacctt
    20401 cggatctata agaagcagcc tgacctcatc cggagaagcc ttgtaaagga tgctcactat
    20461 aaggcagttg atgcagacac ttttgccgga gccggtcgcg ccagcgatca acagatgcgg
    20521 catctttgct atatcagcca ccaccggttc tcccgaaatg tcttttccca cggcaaatgc
    20581 cagttttgag gtatgctgaa cgaattcatt ggactcaagc acttccctca gtcgtaccat
    20641 gttgatttcc ttgttgggca cttctatgcc aatggcggct ttccccggta taggcgcttc
    20701 tatccttatt ccgggcgcag ccaggttcaa tgctatgtcg tccgacagat tgactatcct
    20761 gctcaccttg acaccggcac tcggctgaag ttcatacctg gttaccgtcg gtccccttgt
    20821 gacatttatg actttagctg aaacaccgaa gctgtacaat gtctcttcaa gtttcttcgc
    20881 gccttcaagg gcctggttgc ggtgggccct tgagtttcct gaatcagaag gctcttctat
    20941 cagcagatca taaggcgggt atgtatattt cgtgttctgc tttttcgcag tcgacggccc
    21001 ttttattacg atctcctgca gaccaggtcc gcggtcttct tcactgccgt catcagcgct
    21061 tttcctggcc ggtttcttct tctcccggga tttccttata tcttcggcga caagctcaac
    21121 aggtgatatc tctggtccgg caaaatcttc atctgttccg gattcaatcc caatttcagt
    21181 ttcgtcctca tcttcaggga caggatcgtt tttcgccgcg cctcccctgg ttctctgctg
    21241 cttttttagt tccctcgccg ttttttcgat cctgaaatct ataactttcg gtttatcgaa
    21301 acgcagcgtt atctcgtccc catcttcctc tatcacaggc tgcgtatcgt ccatatcctc
    21361 ttcgtcaaca cgttttggct tctttttcct tacaaaaagg ccccgtatat tcctgatgaa
    21421 atcggcgatg gatttgttgg tcagcaggat tatatcgatc agtgacaatg ctgtaagtat
    21481 aatgatggtc cccggcacct ggaacgtgac aaggaaagga aggctgataa ggccgccaat
    21541 gacgcctcct cccgacagcg cagtaccatc ggtgtagaac cgttccagcg ccttgatcgg
    21601 agtgaggcct gtatagtttt caggtttata tatccccgtc tgtataagcg ccgatacgag
    21661 cgcaaacaat atcaacacgt acagcggttt tctccttatg tcgagcctgt catacgcgac
    21721 tatgagaagc gctccgtaca ggatgaccag aggaggtatc aggaacgcag gtatccccag
    21781 tattccgaga gacgcttctc tcaggaacat gccgaactgt cctatcgagt tctttatata
    21841 gaagctgagc agcatcagta ctccgaaagc gagcaccagt attccctgga tttcgttaaa
    21901 gtatgcaact tgcacctgct ttgaagccct gcgttttttc ttctgtactt tcttgctttt
    21961 caaaacaaca cccccggtga atgattcaaa taagcgcatg aatttcagga tgcatatccc
    22021 ggcagttgat cggtcagcaa atatcacagg gtcgtccccc gattctccaa tatcactata
    22081 ccatattcct gcctcatatt ccagagttgt acagcagcgc tctcctgcct gcgataacgt
    22141 tggctttttc cgtattctat acataagccg gtatattgtc ccctaattct gtacaggcat
    22201 gtttcgacag caaattcaat atgttgtatt atttcaacaa agaaaaaagg attttagcat
    22261 tttatgtcta attattacat aaacaatatt tatttatgtc taattattgc ataaacaata
    22321 tttatttatg aaggttggta tcgaactatg aaaatactat cttcaatagc agagtctatc
    22381 aaatcagttc cgggcttttt gaagaacatc ccgaagtttt tcaaggatat aaggaaaata
    22441 cgcacaaaac tgatccttgt attcatgata ccgatcctgc tgatagcagt acagggcatc
    22501 atgacctata ccaattcttc aaggatggcc agggaaaaca tgacggaatc attcgtgtca
    22561 tcgatggaga acagcgggaa atacctcgag gtcatactcc agacgatg
//
LOCUS       DAOSCC010000011        20632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1837_length_20632_cov_4.198766, whole genome shotgun sequence.
ACCESSION   DAOSCC010000011 DAOSCC010000000
VERSION     DAOSCC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20632
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_20632_cov_4.198766"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1674
                     /locus_tag="PLG72_01415"
     CDS             172..1674
                     /locus_tag="PLG72_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOJ79495.1"
                     /translation="MLKYVFLVLFIAVMILVGIISRRKIRNVQDFFLGGRKMGPWLSA
                     FAYGTTYFSAVIFIGYAGKIGWNFGISATFIGIGNALLGSLLAWLILARRTRKMTHEL
                     GVSTMPEFFEKRYDSKAMKIVTALIIFIFLVPYSASVYQGLAYLFETTFGIPFIYCMA
                     AMALLTGVYLLLGGYVATALNDFIQGIIMLAGVAFMVFYILSSPEVGGLSGGLQKLSQ
                     IPGDGPGLVSVFSAQPLNLLGLIVLTSLGTWGLPQMVHKFYAIKDDQAVKRGTVISTV
                     FAFIIAGCAYFVGAFGRLFLNNTMPVGANGRPNADMVMPMVLEKAMPDALIGIIVILV
                     LSASMSTLSSLVLVSSSAISLDLVKGTLFPDMKKENVMLLMRALCGLFVICSFMVAVT
                     PNSILALMSFSWGTVAGAFLAPFLYGLYWKRTTKAGAWAGFISGFAVSLAGAIIYRMD
                     AAVAPNIGAVAMLVSLAVVPVVSLMTAKMPQKQLDRVFGKTEAGKLEVEM"
     gene            1952..3694
                     /gene="iorA"
                     /locus_tag="PLG72_01420"
     CDS             1952..3694
                     /gene="iorA"
                     /locus_tag="PLG72_01420"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOJ79496.1"
                     /translation="MKELLLGNEAVARGAYEAGVRVATAYPGTPSTEITENIAKYDEI
                     YSEWSPNEKVALEVAIGASVAGARSICSMKHVGLNVAADPLFTASYTGVNGGLVIMVA
                     DDPGMHSSQNEQDSRFYARASLVPMLEPSDSQECKDYVKTAFEISEKFDCPVIVRLTT
                     RVAHSRSLVELEGRNDIELKPYTKDPSKYVMMPAMARGRHLAVEKRMAELRSYAETTP
                     LNRIEWGSREIGVITSGISYQYAREALGDVSYLKLGMVYPLPEGLIREFVSKVDKLYV
                     IEELEPFLENQVKKMGIKVTGKELLPVTGEYSATLLREKLRGIKFEKVYDIDLQSPAR
                     PPVMCPGCPHRGTFHVLKKLKLTVTGDIGCYTLAALPPAQSMDTCVCMGASIGMAHGM
                     EKARGREFGKKTVAVLGDSTFIHSGITGLIDIVYNKGCSTVIILDNSITGMTGHQHNP
                     TTGFTIKGEPTKQVDLEMLAKAVGIERVRIADPFDIKNFEKVVKEEVEADEPSVIIAQ
                     RPCALLKQVRYEGPQRINENCRKCRSCMRIGCPAIVDKGDAIEINSALCVGCKLCSNI
                     CAFNAIEKAGDNNG"
     gene            3687..4262
                     /locus_tag="PLG72_01425"
     CDS             3687..4262
                     /locus_tag="PLG72_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOJ79497.1"
                     /translation="MDDLNIMIVGVGGQGTLLASRLLGKVAQKQGYDVKVSEIHGMSQ
                     RGGSVVTYVKMGRKVYSPIIEKGEADFLLAFEKLEALRWIDYLKKDGTVVLNDQEIDP
                     MPVITGSTMYPEGIIEKLAEAGAHVRSIDALGIARECGNIKAVNMVLIGLLAGTSSIS
                     KDIWLESLKEAVPQKFLDVNVRAFNAGYMVR"
     gene            4505..5806
                     /locus_tag="PLG72_01430"
     CDS             4505..5806
                     /locus_tag="PLG72_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ79498.1"
                     /translation="MLYWNQSRECMPREEMQKLQNERLIKTVKRVYHNVPFYRARMQE
                     KGIEPGDIKGIEDLPKLPFTYKQDLRDNYPFGTFAAPLSEIVRIHASSGTTGKKTVVG
                     YTRSDIERWSEVMARTLVSAGATKESVIQVAYGYGLFTGGLGAHYGAEKIGASVIPIS
                     GGNTRLQVEIMKDFGTTILACTPSYALYMAEEIEEAGIKLSELKLKAGVFGAEPWSEA
                     MRNEIERRLGILAIDIYGLSEVIGPGVASECPCKCGLHVQEDHFIPEIIDPETEEVLP
                     PGSTGELVFTTITKEGLPLIRYRTRDISSLDYSACACGRTTVRMSKVSGRTDDMLIIR
                     GVNVFPSQIESVLLDMGETAPHYQIIVDRVSNLDVMEVWVEMTPTMFSDEVKRIEDLE
                     KNIRKRIEDTLGISVKVKLVEPKSIERSQGKAKRVIDKRRI"
     gene            5888..6319
                     /locus_tag="PLG72_01435"
     CDS             5888..6319
                     /locus_tag="PLG72_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ79499.1"
                     /translation="MFVKQISIFLENKSGRLAEVTSILGDNGIDISALSLADTTNFGI
                     LRLIVNDPDKAEEVLRKDGFTTSVTSVTAIGVKDEPGGLAVALRILKDAGIEIEYMYA
                     FVSKKEDEALVILKTGDIGKTAEVLSSNGINVLSAEEVYNL"
     gene            complement(6431..8935)
                     /locus_tag="PLG72_01440"
     CDS             complement(6431..8935)
                     /locus_tag="PLG72_01440"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOJ79500.1"
                     /translation="MLKSIAGKLPDVLLSVLVSFSITYALTSATGFTYPPVPTLLIII
                     AITAVLIVAFYNRTTSVITAVLLGIVFFSAAVYAIWFVKPHRVIRFFDDYFYWLYDFI
                     LYPDVPDPTYQLISVIGLCFLVCVLSYVFVIRRFSFFVILAAGAGIFAVQASYRVYVT
                     LVPFYVYLAAVLMSYLKHVYLVKSSKAENDYVKPPALLLWSLPVSVVLILLASSIQAS
                     NYPIQWKWLDQKINSAYNYLRNKFDYETFDYFSLSASSGFGDSDDFLGGRVRLDKTNV
                     LYVTTSRRIYLRGATKDVYTGNRWVNSVEELTPVEGEPFSLYNDTEEMLEGLKILAGS
                     DELPDGLLSLNPITVTFLNLKTKSLFIPPKVSSFEPYSSDINSFISDTGDLSAEKRLS
                     KNFQYGLMAYVPTTGSEQFHEILRKSKRGLYAEYLDKHKSLVISSLSVSGKPVIVYPD
                     GTITELSDDISSEINGLIDSNTFAGVVIRSLDEFRQRYNSVRRFKENSDAVYEKYLQL
                     PDELPQRVKDLAASLVIAADSDYDKAKAIEKFVAENYAYNLDVRSTPRDRDFVDYFLF
                     DLQEGYCSYFASAMTILARCVGLPARYVEGYMLPPDPVKDRHDAYVVTNLQAHAWTEI
                     YFEGYGWLPFEPTPPFRSAYYTVDLHENVNLSQYYNPAYEDYMEMMKRYYTQGGSGFT
                     NPGTYRDNADPEPDMIVLWTLCVIGCLFLLLVIVNAVRSKVRLFRLAALPPRTSILKH
                     YDYYIGLLKLAGFAIEPAETPYQYSARIDKLLFFSPVRFKAITDIFVKIRYSTHDATD
                     KEKRLFTEFNAGFLSEIKVSMGKMKYFAFKYILGRI"
     gene            complement(8955..10154)
                     /locus_tag="PLG72_01445"
     CDS             complement(8955..10154)
                     /locus_tag="PLG72_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ79501.1"
                     /translation="MKHTRILFICLFASALVFTYFNGGKIPYMCLYTMLLLPVFSILY
                     TLVIYLRFKFGQDLDKRFVTKGDKVNFLFSINNEDFFLYPYLKVSFYGSNTIFENQYQ
                     IRNFSLPPFSGRNYAFELQCNYRGNYEIGVDSVEIEDFFGLIKFRYNVGEPKYVTVYP
                     RIICLEKFRLKTDYMSETHTMLNSRDEDMTTVSDVRKYQYGDSLKRIHWKLTARTREL
                     MVKKFQSTSETNTLMLLDLRRNYFPPAKNIMLEDKLIEAVVAVLYYCLNKWIPVELVY
                     FSETLYSIQAKNHLMFNEIYETLAKVKFTDDVAVEDLLSIYTESVRMNSNIIVFTANL
                     GYDLYNQLYKTASSGYNVSLVYISPEKITGVRDPEIENILTSLPEIGIECYRIGIDDD
                     IKAILES"
     gene            complement(10156..11097)
                     /locus_tag="PLG72_01450"
     CDS             complement(10156..11097)
                     /locus_tag="PLG72_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ79502.1"
                     /translation="MNDSVRVLRNIIENVENVIVGKRSAVELAVISLICNGHVLIEDV
                     PGVGKTSLVSALARSINASFRRIQFTPDILPSDITGFSVYNQKTGDFEYRPGAIMSQI
                     ILADEINRTSPKTQASLLEVMEERQVTVDGVTYKVPKPFMVLATQNPVEYIGTYPLPE
                     AQIDRFFMKISIGYPTNSEESYILSRFQHSNPLDKLMPVAESNDIISLQEEVKNIHVD
                     KSLSNYIVEIVSQTRKHPDVTLGSSPRGSLALFRASQAWAFYNGRDFVIPDDIRKMVS
                     PVLSHRLMLKQEARLKKINPDEILNNIVSRINVPVVS"
     gene            11321..12562
                     /locus_tag="PLG72_01455"
     CDS             11321..12562
                     /locus_tag="PLG72_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOJ79503.1"
                     /translation="MKAEVLAVGTELLMGQIANTNAQYISSRLPDVGIGVYFHDVVGD
                     NPERLKQSFLLAMSRSDVIILTGGLGPTQDDITKEIVSEVLGRRLLLDRESLDRIEEF
                     FRRRNRMMTQNNIKQAYFPEGCTIIRNDNGTAPGCIIEENGKTIVILPGPPSEMKPMF
                     EETVMPYLAAKSEYRLESVYLRVFGIGESSVEDALLDLIDGQTNPTIATYAKDGEVTL
                     RITARHPKNGSGTDLIRPVADEIKRRFGDAVYSDDDRSLEEVVAGLLMEKKKTISLAE
                     SCTGGLLAARLTDIPGISSVFDRAAVTYSNRAKMEELGVRKETLERYGAVSEQTAKEM
                     ALGIRKVSGTDLGVSVTGIAGPGGGTADKPVGLVYTALAHKDGVIVKRLDLWGDRAKI
                     RNMTCLHVFDMIRRCLISMEG"
     gene            12589..14169
                     /gene="murJ"
                     /locus_tag="PLG72_01460"
     CDS             12589..14169
                     /gene="murJ"
                     /locus_tag="PLG72_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ79504.1"
                     /translation="MANDSENSISTAAIVVMGSIILGRFTGFLRETVLSWKVGLSWVQ
                     DAYVAAFTVPDIVYMLLVGGTISAALVPFLSGKVEKGEEKDGWLAASSFINVITLIMA
                     GICVFGIIFADRIIPAVAPGFTDSSPQTRELAVKLSRILFPSVVFIMLAGICNGVLNS
                     YRKFTAAAFGPSLYNIGCTVSIYLFADTDPESMTKAALFVTLSALGYFLLQLLFSIRK
                     FKFYRPVISIADEGFRKLFGQAVPSLLNSATAQLNTVISTAFVSLSAAEGSLAAFRNA
                     NTLWQMPHGIFAVGIGTAVLPSLSGRYATGGYKEYKTLLMKTLTSVLFFAIPSSVGFL
                     VLREQLVRAVYKWGGRFTEENVPIVAEILAFFCIAMITHSTVTILNRAFYAAQDTRTP
                     LAAGMISVVLNLALGYVFYEYTDLGAAGMAFSYSLISFVNSLLLLLLMGKRLKGINAG
                     ELAGFLIKSVLSAAIMGAVLWGIQEIMPYPERKALQLLYLAMEIIAGCVVYLIMMLIL
                     RSQDAIYLVNNIRQRLKL"
     gene            14266..15324
                     /gene="recA"
                     /locus_tag="PLG72_01465"
     CDS             14266..15324
                     /gene="recA"
                     /locus_tag="PLG72_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ79505.1"
                     /translation="MLERKKALEMALSQIEKQFGKGAVMRLGEEAHLNIETIPTGAIG
                     LDIALGVGGVPRGRIVEIFGPESSGKTTVALHIVAEAQKMGGDAAFIDAEHALDPAYA
                     KNLGVDIDNLIVSQPDTGEQALEIAEALVRSGAIDVIIIDSVAALVPKAEIDGEMGDA
                     HVGLQARLMSQALRKLAGVVSKSKTTAIFINQLREKVGIMFGNPETTPGGRALKFYAS
                     VRMDVRRIDSIKQGTDVIGSRTRVKIVKNKVAPPFREAEFDIMYGTGISKEGSILDVG
                     VSMDIINKSGAWFSYNGQRIGQGRENAKQFLKENPEVAKEIEMKIRENCQQAFARNTT
                     GIHAEDNDGAEDMDFDIE"
     gene            15360..15992
                     /locus_tag="PLG72_01470"
     CDS             15360..15992
                     /locus_tag="PLG72_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOJ79506.1"
                     /translation="MEITSVERNRKYKDKLSVFIDGKFSFNISEDDYLSLNLYEKSEI
                     DEKTIDYIKNTINFQEAKSRAVRYLSLRIRTEKEVRDKLRNEGYDRDCISRVIDELKA
                     IGYINNQLYAQKYVYDRSKLKPMSKKMLKLEMMSKGIPEETAEEVLADWKAEDSDVAR
                     SLVKRKFGKYDLRDEKIMKKAYMFLAHRGFSRDTIKEVLREFDTEISDEL"
     gene            16185..17522
                     /gene="rimO"
                     /locus_tag="PLG72_01475"
     CDS             16185..17522
                     /gene="rimO"
                     /locus_tag="PLG72_01475"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ79507.1"
                     /translation="MGKKIGMVSLGCPKNLVDSELMLGKLRSEGFEIVNDADAADVII
                     INTCGFIESAKQESINTILEMAALKERNCELLVVAGCMAERYRREILDEIPEVDVILG
                     TGSYHEIADAIKEAYSGKRVSRHQELADVSYLDGERIVTTGKGYAYLKIAEGCDNRCT
                     YCIIPALRGPYRSRKMESIIEEASRLADAGIKELIVTAQDTTRYGLDLYGRKMLPELV
                     RNLSSIETVEWIRLLYCYPEEMDTALLKEIANNDKVCKYIDIPIQHASQAVLRKMGRR
                     GSISDICSLIDTIRDEIPDAVIRTTVITGFPGETEEDFETLAEFIKKYRFDRLGTFMY
                     SKEEGTAAYNMPDHVPSRVKASRYNRLMKIQKRISAELNRQRLGKVYKVLVEGVADDG
                     IFYYGRSYAEAPEIDGLIYFTSEEPLEMNQFVNVRILDTGEYDLTGEAQHESS"
     gene            17509..18126
                     /gene="pgsA"
                     /locus_tag="PLG72_01480"
     CDS             17509..18126
                     /gene="pgsA"
                     /locus_tag="PLG72_01480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOJ79508.1"
                     /translation="MNLPNKITLSRILLVPVLMIFIIPIPDVLINAEILSFMKPQMIA
                     VNEFLESFGRYIAAGIFIIASSTDAVDGYIARKRQMVTRFGKFLDPIADKLLVTAALI
                     ALVQSGDINGWVAMIIISREFIVTGLRLVAAGEGIVIAASNLGKLKTVLQDVAIVASL
                     LNNFPLSLFTNFQFDDYAMLLAVIITIISLYDYLKKNSGVIDTSK"
     gene            18300..18875
                     /gene="fapR"
                     /locus_tag="PLG72_01485"
     CDS             18300..18875
                     /gene="fapR"
                     /locus_tag="PLG72_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HOJ79509.1"
                     /translation="MSRSSSSKRERQQVLIEKIKEDPFLTDEELAELLNVSVPTIRLD
                     RLEMGIPELRERIKSVAEKNYSKVKSLQGGEIIGELIDITLGKYGVSILETDDSMAFE
                     RTKIIRGHFIYSLAESLAIAVIDADVALVGVANIKYKNPVYSGTRLIAKAEVKQIRGS
                     RYIVWVRIYDRQVEMFRGKFILVSLEKPQEK"
     gene            18936..19931
                     /gene="plsX"
                     /locus_tag="PLG72_01490"
                     /pseudo
     CDS             18936..19931
                     /gene="plsX"
                     /locus_tag="PLG72_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            19939..>20632
                     /locus_tag="PLG72_01495"
     CDS             19939..>20632
                     /locus_tag="PLG72_01495"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOJ79510.1"
                     /translation="MAKGQKENVSVGILGIGSCVPEKILTNSDLEKMVDTSDEWIIQR
                     TGISERRIMDKNTPTYTLAVEAAKQAIMDSGISPEQIGLILVATSTPDFLTPSTSCIV
                     QKETGAVNAAAFDIMAACSGFIYGLTIAKQFVETGYYEYVLLVSSEGMSKAVDWTDRK
                     TCVLFGDGAGAVVIGKVEEGYGILSTYLGADGASGHHITIPCLHLPEEELEKRKDYEN
                     KIAIYQDGHEVFK"
BASE COUNT         5767 a         4455 c         5498 g         4912 t
ORIGIN      
        1 gagtcaaaac atgcaggtaa ccgcgaatca atgactggaa gaacacagga cagcgaaaaa
       61 acttcttccc attttcatat aaatatagta taatactttt gatttttcga agaatggcag
      121 gtattgcagg taaggctgat caaatcaaga tagcacggaa ggatggtagg tatgcttaag
      181 tatgtctttt tagttctttt catcgctgtc atgattctcg tagggatcat cagcaggaga
      241 aaaatccgga acgtacagga tttctttctt ggcggcagga agatgggacc atggctttcg
      301 gcgttcgctt atgggaccac atatttttca gccgtcattt tcattggtta tgcaggcaag
      361 attgggtgga attttggcat atccgctaca ttcataggca tcggcaatgc attgctgggc
      421 agcctgcttg catggctgat tctggcaagg cgtacaagaa agatgacaca tgaactcgga
      481 gtgtcgacga tgcctgaatt ttttgaaaag cgttatgaca gcaaagccat gaagatagtt
      541 acggcgctga tcatattcat tttcctggtg ccgtattcgg cgtctgtata ccaggggctt
      601 gcatatctgt tcgagaccac gttcggcatt ccattcatat actgcatggc ggccatggcg
      661 ctgcttaccg gcgtatacct gctgctgggc ggctatgtcg caacagcgct gaatgatttc
      721 atacagggca taataatgct cgccggtgtg gcattcatgg tgttttacat cctatcaagc
      781 ccggaagtcg gaggcctgtc cggtggatta cagaaacttt cgcagatccc aggtgacgga
      841 cccggattgg taagcgtatt cagcgcacag cctttgaacc tgctgggcct gatcgtgctg
      901 accagccttg gcacatgggg acttccccag atggtgcaca agttctatgc aataaaggat
      961 gaccaggctg ttaagcgagg aactgtcata tccaccgtat tcgcatttat catcgctggc
     1021 tgtgcgtatt ttgtgggagc tttcggccgt cttttcctca acaataccat gcctgtagga
     1081 gcaaacggaa ggccaaatgc ggacatggta atgcctatgg tgcttgaaaa ggcaatgccg
     1141 gatgcgctga tcggtatcat agtcatactg gtgctttctg catcgatgtc aactctttca
     1201 tcactggtgc ttgtatcgag ttccgcaatt tcgctggacc tggtgaaggg gacgctgttc
     1261 ccggacatga agaaggagaa tgtgatgctc ctgatgagag cgttgtgcgg attgtttgtg
     1321 atatgctcgt tcatggtagc tgtgacacct aattccatcc ttgcgctgat gtccttttca
     1381 tggggcactg tggccggcgc tttccttgct ccgttcctgt acggactgta ttggaaaagg
     1441 acgaccaaag caggagcatg ggcaggcttc atcagcggtt ttgcagtgtc gctggcagga
     1501 gcgataatat acaggatgga tgcggcggtt gcccccaaca tcggcgcggt ggctatgctc
     1561 gtatcacttg cagtcgttcc tgtagtcagc ctgatgactg caaaaatgcc tcagaaacag
     1621 ttggacaggg tgtttggcaa aactgaggca ggaaagcttg aggtggagat gtaaaagact
     1681 gttgctgggc tgttggcggc gccggcgcat gtctatggta ctttccggcg cctgatacag
     1741 gtatccggaa gttatggatg cacaactttc ctgtcatggc atgatcatga taacgggaca
     1801 tcccattggt gggcttgtcc ctttttgcta agggtttaca aaaaacacac aaatgattaa
     1861 aataaaacaa gacgaaaaaa ggtctggctt ttgcggaagc cattaaacag agaaaataaa
     1921 aagacagaaa agaaatggat gaggagatta tatgaaagaa ttgcttttgg gaaatgaagc
     1981 agtcgcaagg ggggcatacg aggcaggcgt gagggtcgcc actgcgtatc ctggaactcc
     2041 aagcacggag ataaccgaaa acattgccaa gtatgatgaa atctacagcg aatggtcacc
     2101 caacgagaaa gtggcgctgg aagtcgctat aggcgcatcg gtggcggggg caaggtctat
     2161 atgctcgatg aagcacgttg gcctcaatgt agcggcagat ccgctcttta ccgcatccta
     2221 tactggagtg aacggagggc ttgtcataat ggtcgcagac gaccctggca tgcacagttc
     2281 ccagaacgaa caggattcaa ggttttatgc ccgtgcgtcg ctggtgccga tgctggagcc
     2341 gtctgacagc caggaatgca aagattacgt aaagacagcc tttgaaataa gtgaaaagtt
     2401 cgactgccct gttatcgtga ggctaaccac acgggtcgcc cattcacgca gtcttgttga
     2461 actggaagga agaaacgata tcgagctcaa gccgtataca aaggatccgt cgaagtatgt
     2521 gatgatgcct gccatggcga gaggacggca tcttgcagtt gaaaaaagga tggcggagct
     2581 gagaagttat gctgaaacga cgccgttaaa caggatcgaa tggggaagca gggagatagg
     2641 cgtcataaca tcgggtattt cataccagta cgcccgtgaa gctctcggcg acgtttcata
     2701 cctgaagctt ggaatggtat atccgctgcc ggaaggtctg atcagggagt tcgtttccaa
     2761 agtggataaa ttgtacgtta tagaggagtt ggaaccgttt cttgaaaacc aggtcaaaaa
     2821 gatggggatc aaagtgaccg gcaaagaatt gctgcctgtc acaggagaat attcggccac
     2881 tttactcagg gaaaagctgc gcggcatcaa gtttgaaaag gtatatgaca ttgatttgca
     2941 gtcgcctgca aggcctcctg tcatgtgccc cggatgccct cacagaggca ctttccacgt
     3001 actgaagaaa cttaagctga cagtaacggg agacataggg tgttacacac tggccgcgct
     3061 tccgccggca caatccatgg atacatgcgt atgtatggga gccagcatcg gaatggctca
     3121 cggcatggag aaggcacgcg gcagggagtt cggcaaaaag actgtggcag tgctcggcga
     3181 ttccacgttc atccactccg gtataaccgg gttgatagac attgtttaca ataagggctg
     3241 ttccacggtg ataatactgg acaattccat cacgggcatg acagggcatc agcacaatcc
     3301 caccacaggc ttcaccatca agggagaacc tacgaaacag gtcgaccttg aaatgctggc
     3361 aaaagcagtt gggatcgaaa gggtaaggat agctgacccg ttcgacatta agaatttcga
     3421 aaaagtcgtg aaggaagagg tcgaagcaga cgagccgtct gtgataattg cccagaggcc
     3481 gtgcgcactg ctgaagcagg tgcgttacga aggcccgcag aggataaacg aaaactgccg
     3541 gaaatgcaga agctgcatga ggataggatg tccggcaata gtcgacaagg gagacgcgat
     3601 agagatcaac agcgcgctgt gtgtcggctg caagctctgc agcaatatat gcgctttcaa
     3661 tgcaatagaa aaggccggtg ataataatgg atgacctgaa tatcatgata gtgggagtcg
     3721 gaggtcaggg aacgctgctt gcaagcagat tgctgggcaa agtggcccag aagcagggat
     3781 atgatgtaaa agtttctgag atccacggta tgtcgcagag aggcggcagt gtcgtcacat
     3841 atgtcaaaat gggcagaaag gtttattcac caattataga aaaaggcgag gcggacttcc
     3901 tgctggcttt tgaaaaactt gaagcactgc gctggataga ttaccttaaa aaggacggaa
     3961 cggtggtgct gaatgatcag gaaatagacc ccatgcctgt catcaccgga agcaccatgt
     4021 atccggaagg gataatcgaa aagcttgcag aggcgggcgc acatgtgcgt tccatcgatg
     4081 cacttggaat agcaagggaa tgcggcaaca taaaagctgt aaatatggtt ctgataggcc
     4141 ttctggcagg gacttcctcc atctcgaaag atatctggct ggaatccctt aaggaggcag
     4201 tgccgcagaa attcctcgat gtcaacgtca gggcgttcaa cgcgggatat atggtacggt
     4261 gacactcctc agcaggaagg tattcctttc ctgatgaggg atgccatacg cagaaatgca
     4321 gtgtcagttg tgagaaataa atgagaacgg tattttacgt aattttatcg tgtatggtgt
     4381 aatcagcttt tttctgtaat tcagcctgtt gcttttgcgt ggggcgggca ggtgcccgta
     4441 aaccctgaaa gctattctcg cgggaggata aagggcataa aaaatatcag aggagggttc
     4501 ttctatgttg tactggaacc agtcgcgtga atgcatgccc agagaggaga tgcaaaagct
     4561 tcagaatgag cggctgatca aaactgtcaa aagagtgtat cacaatgttc ccttttacag
     4621 ggcgaggatg caggagaaag gtatagaacc cggggacata aagggtatcg aagacctccc
     4681 gaagcttccg tttacttaca agcaggatct cagggacaat tatccttttg gtacatttgc
     4741 agcgccattg tctgagatcg ttaggatcca tgcctcgtca ggaacgaccg gtaaaaagac
     4801 ggtagtcgga tataccagga gcgatatcga gagatggtcc gaggttatgg caaggactct
     4861 tgtcagtgcc ggggcgacaa aagaatcggt aatacaggta gcatatggct atggtctttt
     4921 caccggcgga cttggtgccc attatggcgc cgagaaaatc ggagcgtccg tgatcccgat
     4981 ctcgggaggg aataccaggc tccaggtcga gataatgaag gacttcggca ccacgatact
     5041 tgcatgtacg ccttcctacg cgctgtacat ggcggaagag attgaagaag caggcataaa
     5101 gctcagcgaa ctgaagctga aagccggcgt attcggagca gagccatggt cggaggcgat
     5161 gaggaatgaa atagaaagaa ggctgggaat acttgcaata gatatttacg ggctcagcga
     5221 agtcattggt ccgggcgtgg catccgagtg tccctgcaag tgcggcctgc atgtgcagga
     5281 agaccatttc atacccgaaa ttatcgatcc cgaaaccgag gaagtactgc ccccgggatc
     5341 cacgggcgag ttggtgttca ccactatcac gaaggaaggc ctgccgttga tacgttacag
     5401 gaccagggac atatcttcgc ttgattattc ggcttgtgca tgcggaagga ctactgtcag
     5461 gatgagtaag gtatcgggac gtactgacga catgctgata atacgaggag tgaacgtatt
     5521 ccccagccaa atagaaagcg tactgctcga tatgggagaa accgcgcccc actaccagat
     5581 catcgtggac agggtttcta atctcgatgt catggaagta tgggtcgaaa tgacgccgac
     5641 tatgttctcg gatgaggtaa agaggatcga ggatcttgag aagaatatca gaaagaggat
     5701 agaagataca cttgggataa gcgtgaaagt caagcttgtc gaaccaaaat ccatagagcg
     5761 cagccagggc aaagccaaaa gagtgatcga caaacgcagg atatgacaaa gcataataaa
     5821 tatgcggcgt tattgctgct gcagtatatc cctgatcgta tccaattttg aacgggaggt
     5881 aggatccatg tttgtaaagc aaatatcaat ctttctggaa aacaaatccg gacggcttgc
     5941 ggaagtaacc tcgatacttg gcgacaatgg aatagacatc agtgctttgt cattggcgga
     6001 cacaacgaat ttcggcatac tcaggctcat tgtgaacgat cctgataaag ccgaggaagt
     6061 attgcggaaa gacggattca caacgagcgt gacctcggta acggcaatag gcgtcaagga
     6121 tgagcccggc ggccttgctg tggcgctgag gatccttaag gatgcaggga tcgaaataga
     6181 gtacatgtac gctttcgtca gcaaaaaaga ggatgaagcc ctcgtgatcc tgaagacggg
     6241 agatatcggg aaaacggccg aggttttgag ttcaaacggg atcaacgtac tttcggcaga
     6301 agaagtatat aatctttgat cgatcatcct ggtttacagc tgtaatagat atctgtataa
     6361 aaaacgaaag ccgttcattg ctgaacggct tttggatttg atgcgatatt ttcggtttga
     6421 cactatagct tcatatcctg ccaaggatat atttaaacgc gaaatacttc attttaccca
     6481 tggagacctt tatctcactg agaaaaccgg cgttgaactc ggtaaacagc cgcttttcct
     6541 tatccgtggc atcatgtgtg ctgtatctta tctttacgaa gatgtcggtt atcgctttga
     6601 accggacagg actgaagaac agaagcttat cgatccttgc agaatactgg tacggcgtct
     6661 ccgccggttc gatggcgaaa ccggcaagtt tcagcagccc gatgtaatag tcataatgtt
     6721 tgagtatgga tgtccttggc ggcaaagctg ccaacctgaa caacctgacc ttgcttcgga
     6781 ctgcattgac gataactaaa agcaggaaca ggcatccgat aacgcagagg gtccacagta
     6841 cgatcatatc gggctcagga tccgcattgt ccctgtaagt gccgggattc gtgaaaccgc
     6901 ttccgccctg ggtgtagtat cttttcatca tttccatata atcttcataa gcaggattat
     6961 aatattggct taagttaaca ttttcatgca gatcgaccgt atagtaggcg gacctgaacg
     7021 gaggcgtcgg ttcaaaggga agccagccgt agccttcgaa atagatctcg gtccacgcat
     7081 gggcctgcag gtttgtgacc acataagcgt catgcctgtc tttcaccgga tctggaggaa
     7141 gcatgtagcc ttcgacatac cttgcgggaa ggcctacaca tctggcaagg atggtcattg
     7201 cagatgcgaa gtatgaacaa tagccttcct gcagatcgaa caggaaataa tctacaaagt
     7261 ccctgtccct tggagtcgaa cgcacatcca ggttatatgc ataattctcg gcaacgaact
     7321 tctcgatggc cttggctttg tcatagtcac tgtcagccgc tatgaccagc gatgctgcca
     7381 ggtcctttac cctctgggga agttcatccg gaagctgaag gtatttctca tatacagcat
     7441 cgctgttttc cttgaacctg cggacactgt tatatctctg gcggaactca tcaagggatc
     7501 ttatgactac tcccgcaaaa gtattggaat cgataagtcc atttatctct gaagaaatat
     7561 catccgacag ttcggtgatt gtgccgtctg ggtagacgat caccggcttt cctgataccg
     7621 ataaggatga tataaccagg gatttgtgtt tatcaaggta ctctgcatat aagcctcttt
     7681 tacttttcct caatatttca tggaactgtt ccgatccagt cgtcgggaca tatgccatca
     7741 aaccgtactg gaaatttttc gacaaccttt tttcagcaga aagatcgccg gtatcgctga
     7801 taaaagaatt aatgtcagaa gaatatggct caaaggaact gaccttcggc gggatgaaga
     7861 gcgactttgt cttcaggttg aggaatgtga ccgttatggg attcaaagac agaagaccgt
     7921 caggaagttc atcgctgccg gccagtattt tcaggccttc cagcatttcc tcggtatcgt
     7981 tgtaaagact gaagggttca ccttcaacag gggtaagctc ctcaacactg ttgacccatc
     8041 tgttgcccgt atatacatcc ttggtggcgc ccctgaggta gatccgcctg ctcgttgtga
     8101 catacaatac attcgtcttg tcgagcctga ccctgccgcc caggaaatca tcgctgtcac
     8161 caaagccgga agatgcggaa agggagaaat agtcgaaggt ttcatagtcg aatttgttcc
     8221 tgaggtagtt ataagcactg ttgatcttct ggtcaagcca tttccactgg atcggataat
     8281 tgctcgcctg tatcgaggac gcaagcagga tcagcacgac gctgacaggc aaactccaga
     8341 gcagcagggc aggaggtttt acgtaatcat tttctgcttt tgagcttttt accaggtaaa
     8401 cgtgtttgag atacgacatc agtaccgcag caagataaac gtaaaacgga accagcgtaa
     8461 catacacgcg gtatgatgcc tgtactgcaa atatgccggc gcctgctgcc aatatcacaa
     8521 agaaactgaa tctcctgatg acgaaaacat acgaaagcac gcatacaaga aagcataagc
     8581 cgataacgct gataagctgg tacgtcggat caggcacgtc cggatacaat atgaaatcat
     8641 aaagccagta aaaataatcg tcgaaaaacc ttatcaccct gtggggcttt acaaaccaga
     8701 tcgcatatac tgccgcggag aagaaaacaa taccaagcaa tacagcagtt atcacagagg
     8761 tagtcctgtt atagaaagca acaatgagca cagctgtgat cgcgatgatt atcagcagcg
     8821 tcggcacagg agggtaagtg aagcctgtgg ctgatgtgag ggcatatgtg atgctgaacg
     8881 atacaagcac tgataataat acatccggta atttcccggc aatacttttc aacacgatat
     8941 cattcctccc gctcctagct ttcaaggatc gcttttatat cgtcgtctat accgatcctg
     9001 taacattcga taccgatctc gggaagcgac gtaagtatgt tctctatttc cggatccctg
     9061 acacccgtta ttttctcagg tgagatatat acgaggctta cattgtagcc ggagcttgca
     9121 gttttgtaca gctggttgta aaggtcataa ccaaggtttg ccgtaaacac gataatattt
     9181 gaattcatcc ggacgctctc cgtgtatatg ctgagaagat cctccactgc gacgtcatcg
     9241 gtgaatttga cctttgccag tgtttcatat atctcattga acatgaggtg gttttttgcc
     9301 tggatgctgt atagcgtttc gctgaaataa acaagctcaa caggtatcca tttgttcagg
     9361 caataataaa gaaccgcgac gacagcctcg atcagtttgt cttccagcat gatgttttta
     9421 gccgggggaa aatagttcct tcgcaggtcc agcagcatca gggtgttggt ttcagatgta
     9481 ctctggaact tcttcaccat aagttcccgg gtccgtgccg tcagtttcca gtgtatcctt
     9541 ttcagcgaat caccatattg atacttcctg acgtcactta cggtagtcat gtcttcatcc
     9601 cttgaattga gcatcgtgtg cgtttccgac atgtagtccg ttttgaggcg gaacttctcc
     9661 aggcagatga tcctcggata tacggtgaca tacttgggtt cgccgacatt gtacctgaat
     9721 tttatgagcc caaaaaaatc ttctatctcg acggaatcga ccccgatctc ataattaccc
     9781 ctgtaattgc actggagttc aaacgcatag tttctgcctg aaaacggggg cagcgagaaa
     9841 ttcctgatct ggtactggtt ctcgaaaatc gtgttggatc cgtagaacga taccttaagg
     9901 tacggataca ggaagaaatc ttcattgttt atgctgaaca ggaagttcac tttgtcgccc
     9961 ttggtcacga accgtttatc aaggtcctgc ccgaacttga acctgaggta tatgacgagt
    10021 gtatataaaa tggaaaatac aggcaggagc agcatggtgt aaaggcacat gtaaggtatc
    10081 ttgccgccgt tgaaataggt aaataccagg gcggaagcaa acaggcatat gaaaaggatc
    10141 ctggtgtgct tcatgctagc tcaccaccgg cacatttatt ctggaaacga tattattcaa
    10201 tatttcatcc gggtttatct tcttaagcct tgcttcctgc ttgagcatga gcctgtgtga
    10261 taataccggg gacaccattt tcctgatgtc atcgggaatc acgaaatcac ggccgttgta
    10321 gaatgcccat gcctgtgacg ccctgaacag ggcaagcgaa cccctggggc tcgatcccag
    10381 cgtgacatcc gggtgcttcc tggtctggct cactatttcc actatataat tgctgaggct
    10441 cttgtcaaca tgtatgtttt tcacttcctc ctgaagggat attatgtcgt tgctttcggc
    10501 aacaggcatc agtttgtcaa gaggattcga gtgctggaat cttgaaagga tgtagctttc
    10561 ctccgagttg gtcgggtatc ctatggatat cttcatgaaa aagcggtcta tctgtgcttc
    10621 aggcaacgga tatgtgccga tgtactcaac agggttctgc gttgcaagta ccatgaacgg
    10681 tttcggcact ttgtatgtaa ctccgtcgac cgttacctgc ctttcttcca tgacctcgag
    10741 aagactggcc tgtgtcttgg gcgacgtcct gttgatctca tcggcgagga tgatctggct
    10801 cattattgcg ccgggcctgt attcgaaatc gccggtcttc tggttgtaga ctgaaaagcc
    10861 agttatatcg gagggaagta tgtcaggtgt gaactgtatc ctccggaatg atgcgttgat
    10921 ggacctggca agggctgaaa ccaggctcgt cttgccgaca ccaggtacgt cttcgatcag
    10981 gacgtggccg ttgcagatca gcgagatcac tgcaagttcc acggcggaac gcttgccaac
    11041 gataacattc tccacatttt cgataatgtt cctgagaact ctgacagaat cgttcataac
    11101 acccatcctt ctgtttatgt aattttaatt ttatgatagt atagcggtta tttttgatga
    11161 agtagttttt ttcaggagaa ttgtagtgtt ttttttcaac tggcggacag aggcaatctc
    11221 atgtttacat taaaaaaact ggattatata atagaaacga atgccggaaa ctgtgatgac
    11281 acggcccgac agatacagat caacaggaga tggagaacat atgaaggcag aggtccttgc
    11341 agtcggaaca gaactgctca tggggcagat agcaaatacg aacgcacagt atatatcttc
    11401 gaggctcccc gatgttggta tcggggtata ttttcacgac gtagtcgggg acaacccgga
    11461 aaggcttaag caaagctttt tgctggccat gtcacgttcc gatgtcatca tactgaccgg
    11521 cggtcttgga cctacgcagg atgacatcac caaggagatc gtatcggaag tcctcggacg
    11581 caggctcctg cttgaccggg aaagccttga caggatcgag gaattcttca gaagacgaaa
    11641 cagaatgatg acacagaaca acatcaagca ggcttatttc cccgaaggat gcactatcat
    11701 caggaacgac aacggtacgg ctcccggctg cataatagag gaaaatggca aaacaatagt
    11761 gatattgcct ggcccgcctt cagaaatgaa gcccatgttc gaggaaacgg tgatgccgta
    11821 cctggctgcc aaatccgaat atcggctcga atcagtatac ctcagggttt tcgggatcgg
    11881 ggaatcgtct gtcgaagatg cgctccttga tcttatagac ggccagacaa atccgacgat
    11941 tgcaacatac gccaaggatg gagaagttac attgcgcata acggcaaggc acccgaagaa
    12001 cggcagcggc acggatctca tcaggccggt ggcagacgag ataaagcgaa ggtttggcga
    12061 tgccgtttac agcgatgacg accgcagcct cgaagaagtg gttgccggac tgctgatgga
    12121 aaagaagaag acgatctctc tggccgaatc ctgcacagga ggccttttgg ctgcaagact
    12181 caccgatatc ccgggtattt cctcggtttt tgaccgggct gctgtgacat acagcaacag
    12241 ggccaagatg gaggaacttg gcgtaaggaa ggaaacactt gaaagatacg gcgctgtcag
    12301 cgaacagact gcaaaagaga tggcactagg catacggaaa gtatcaggaa ctgatctggg
    12361 cgtatcagtt accggtatag ccgggccagg cggaggaaca gccgataagc ccgtaggact
    12421 cgtatacact gcattggccc ataaagacgg agtgatcgtg aaaaggctcg atctctgggg
    12481 agacagggca aagatccgga atatgacttg cctgcatgta tttgacatga taagaagatg
    12541 ccttatcagt atggaaggct gaaaatttgg cgctctggag gacctgaatt ggcaaacgac
    12601 agtgaaaaca gcatatcaac ggcagctata gtggtcatgg gttcgatcat cctgggaagg
    12661 ttcacgggat tcctccgtga aacagtgctc tcatggaagg ttgggctcag ttgggtacag
    12721 gacgcatatg tcgctgcttt tacagtgcct gacatcgtat atatgctgct cgtcggaggt
    12781 actatatcag cggcgctggt cccgtttttg tcaggaaaag tcgaaaaggg tgaggaaaag
    12841 gacggatggc tggccgcaag ttcatttatt aatgtaatta cactgatcat ggcgggaatc
    12901 tgcgtattcg ggatcatttt cgcagacagg ataatacctg ctgtcgctcc tggtttcacc
    12961 gacagcagtc cccaaaccag ggaacttgcc gtgaagcttt caaggatact gttcccgtca
    13021 gttgttttta ttatgctggc ggggatctgc aacggcgtgc tcaactcata caggaagttc
    13081 acagcagcgg cattcggacc ttccttatac aacattggat gtaccgtaag catatacctg
    13141 tttgcagata cggaccccga aagcatgaca aaagccgcat tattcgtaac gcttagcgcg
    13201 ctcggatatt ttctgctgca actgctgttt tcaataagga agttcaaatt ttacaggccg
    13261 gtgataagca tagccgacga aggcttcagg aaacttttcg gccaggccgt tccgtcattg
    13321 ttaaattctg cgacagcgca actgaatacg gtcatatcga ccgcatttgt cagcctgagc
    13381 gccgcagagg gcagccttgc tgcattcagg aacgccaata cattatggca gatgccccat
    13441 ggcatattcg cggtagggat cgggacagcc gtgctcccgt cgttgtcagg aaggtatgca
    13501 acgggcggat acaaggagta caaaactctg ctgatgaaaa cgctgacgtc tgtcctgttt
    13561 tttgcgatac cgtcgtcagt gggcttcctg gtactcaggg agcagcttgt cagggctgtg
    13621 tacaaatggg gcggcaggtt cacggaggag aatgtgccta tcgtggcaga gatactggca
    13681 ttcttctgta ttgcgatgat cacgcactcc acggtgacga tattgaacag ggctttctat
    13741 gcggcgcagg ataccaggac gccgctggcg gcaggcatga tttccgtagt gctcaacctg
    13801 gcgctcggat atgtttttta tgagtatacc gatctcggag cagcagggat ggcgttttcg
    13861 tattcgctga tcagtttcgt gaattccctg ttgctgctcc tgctgatggg gaagaggctg
    13921 aaaggcataa atgccggcga actggcgggt ttccttatca aatccgtatt gtccgcagca
    13981 atcatgggcg ccgttttgtg gggcatccag gaaataatgc cgtatccgga aagaaaagca
    14041 ttacagctgc tgtatcttgc gatggagatc atcgcgggat gcgtcgtata tttgataatg
    14101 atgctgattc tccggtcgca ggatgctatt tatttggtga ataacatcag gcagagattg
    14161 aaattgtaaa aatatgttat attgttattg actattcccg agaaataata tataatctac
    14221 atcagaacaa gtgttcgttc attcggatta aggaggtctg ctcaaatgtt ggaaagaaag
    14281 aaggcattgg agatggcact gagccagata gagaagcagt tcggcaaggg tgctgtcatg
    14341 aggctggggg aagaagccca tctgaacatc gaaacgatac ccacaggcgc gataggtctt
    14401 gatatagccc ttggcgtagg gggcgtaccg agagggagga tcgttgagat attcggacct
    14461 gagtcttcag gaaagacgac cgttgccctg catatagtcg ccgaagctca gaagatgggc
    14521 ggagacgctg ctttcatcga tgcagagcat gcattggatc ccgcatatgc aaaaaacctg
    14581 ggtgtcgata tcgacaacct tatcgtatcc cagccggata caggagagca ggcgctcgaa
    14641 atagccgaag cacttgtaag gagcggagcc attgacgtta tcattataga ctcggtcgca
    14701 gctttggtgc cgaaagcgga gatagacggc gagatgggag acgcacatgt cgggctgcag
    14761 gcaaggctca tgtcccaggc cctcaggaaa cttgcaggcg tcgtcagcaa atcgaagacc
    14821 acggctatat tcataaacca gctcagggaa aaggtcggca tcatgttcgg caatcccgaa
    14881 acgactccag gaggaagggc attgaaattc tatgcatctg taaggatgga cgtcaggagg
    14941 atcgattcca tcaagcaggg gactgacgtg atcgggagca ggacaagggt caagatcgtc
    15001 aaaaacaagg tcgctccgcc cttcagggaa gcagagttcg atataatgta cggtacaggt
    15061 atttcgaaag agggcagcat cctcgatgta ggcgtcagca tggacatcat aaacaagagc
    15121 ggagcgtggt tctcttacaa tggccagagg ataggccagg ggagggaaaa cgcaaagcag
    15181 ttcctcaagg aaaatcccga agtggcgaaa gagatagaaa tgaagataag ggaaaactgc
    15241 cagcaggcat ttgcaaggaa caccaccggt attcatgccg aagataatga cggtgccgaa
    15301 gatatggatt ttgacatcga atgaacaatg tccagagacg atgcgggagg tggctgttat
    15361 tggagataac atcggtagaa aggaacagga aatataagga taaactctcg gttttcatcg
    15421 atggaaagtt ttctttcaat atttccgaag atgactatct ctcgctgaat ctctatgaaa
    15481 aatccgagat cgatgagaag acaatagatt acatcaaaaa tacgataaat ttccaggaag
    15541 caaaatcgag agcggtcagg tacctttccc tcaggataag gaccgagaag gaagtcaggg
    15601 acaagctgag gaacgagggc tatgaccgcg attgcatatc acgtgtcata gatgaactca
    15661 aagccatcgg gtacataaac aatcagctgt atgcgcagaa atacgtatat gacagaagca
    15721 agctcaaacc gatgtcgaaa aagatgctga aacttgagat gatgtcaaag ggtatccctg
    15781 aggagacggc agaagaggtg ctggcagact ggaaggctga agattcggac gtagccagga
    15841 gcctggtgaa aagaaagttc ggcaaatatg atctgcggga tgagaaaata atgaaaaaag
    15901 cttatatgtt ccttgcccac agagggttca gccgggatac cataaaagaa gtactgaggg
    15961 agtttgacac agagataagc gacgaattgt gatctttccc atatatcaga ccaggctttc
    16021 tctacataac ttcagccgag atgctgtgaa aaaacggggt gccgaaggcg ccttttactt
    16081 ttttgccgat ttgaaatggc aaaatcgtga atgggttttt tgtattgtga aaaagttata
    16141 tatgctgtat attatactga gcacggcaac agggagtgtt atgtttgggc aaaaagatag
    16201 gaatggtatc gctgggctgt ccaaagaacc tggtagacag cgaactgatg cttggaaaac
    16261 tgagatccga aggatttgaa atagtcaacg acgctgatgc cgcagatgtg atcatcataa
    16321 atacctgcgg ctttatagag agcgcaaagc aggaatcgat aaacactatc cttgaaatgg
    16381 cggcgctgaa ggaacgcaat tgtgaactgc tggtcgttgc agggtgtatg gcggaaaggt
    16441 accgcaggga gatactcgac gagatccccg aggtggatgt gatactggga acaggcagct
    16501 atcatgaaat tgcagatgcc ataaaggagg catacagcgg gaagcgtgta tcgcggcatc
    16561 aggagcttgc ggacgtatct tacctcgatg gagagcgtat agtcaccacc ggtaaaggat
    16621 atgcctatct gaaaattgcc gaaggatgcg ataaccgttg tacttattgc attattccgg
    16681 ctttacgcgg accgtacagg agcaggaaga tggaatcgat catcgaggaa gcaagccgcc
    16741 tggctgatgc cggcataaaa gaactgatcg tcactgccca ggacactaca agatacggcc
    16801 ttgacctgta cggcagaaaa atgcttcccg aacttgtaag gaatctgagc agtatcgaaa
    16861 cggtcgaatg gatcaggctg ctttactgtt atcccgaaga aatggacacg gcattgctga
    16921 aagagatcgc taacaatgac aaagtgtgca aatatatcga tataccgata cagcacgcca
    16981 gccaggcagt actcaggaaa atggggcgca ggggtagcat ttccgatata tgcagtctga
    17041 tcgatacgat aagggatgag attcccgatg cagtcatcag aacaacagta ataacaggtt
    17101 ttcctggcga aactgaagaa gatttcgaaa cccttgctga atttataaaa aaatacaggt
    17161 ttgacagact tggaacattt atgtattcta aagaagaagg tacggcagca tacaacatgc
    17221 ctgaccatgt gccgtcaaga gtgaaagcat cgagatacaa caggctgatg aagatacaga
    17281 aacgcatatc ggccgaactg aaccggcaaa ggctgggaaa ggtatataaa gtacttgtgg
    17341 aaggtgtcgc agacgacggg atattctatt acggcagaag ctatgctgaa gcaccggaaa
    17401 tagacggact gatatatttt accagcgaag aaccgctgga aatgaaccag tttgtaaatg
    17461 tcaggatact tgataccgga gaatacgatt taacaggaga ggcacagcat gaatcttcct
    17521 aataaaataa ctctttctcg aatacttctg gtacccgtac ttatgatttt catcattccc
    17581 atacctgatg tgctcatcaa tgccgaaata cttagtttca tgaagcccca gatgatcgca
    17641 gtcaatgaat ttctggaatc gttcgggcgt tacattgccg caggtatttt cataatagca
    17701 tcaagtacgg acgctgtaga cggatacatt gcaagaaaga ggcagatggt aacaaggttc
    17761 ggcaaattcc tcgatccgat cgcagataaa cttctggtga ctgcagcact tatcgccctg
    17821 gtgcagagcg gggatataaa cggctgggtc gcgatgatca tcatctcacg agagttcatc
    17881 gtaacaggac tgaggctggt tgctgccggc gagggaatag tcattgccgc aagcaatctg
    17941 ggcaaactga agacagtact gcaggacgtg gctatagtcg cgtcgctgct caacaatttc
    18001 ccgttgagcc tgttcacgaa ttttcagttc gacgattacg ccatgctgct tgcggtcata
    18061 attacgatca tttcattgta tgactacctt aagaagaatt ccggcgtgat cgataccagc
    18121 aaatagacaa ggacgacaac aacggggaga atagccttcc gcagatgcgg accggacatc
    18181 tccccgtttt tttattaaag ttgttgcata cttctgaaat atattatata cttttagtaa
    18241 taggacatag tatcaggtaa taattatagg actaagcatg caaagcaggt gttgacgaaa
    18301 tgagccgctc atcctcatcg aaaagggaaa gacagcaggt attgatcgaa aaaataaaag
    18361 aagatccgtt ccttacggat gaggaactgg cggaactgct caatgtcagc gtgccgacta
    18421 tcaggcttga caggctcgaa atgggtatcc cggaactgag agaaaggata aagagcgttg
    18481 ctgagaagaa ttacagcaaa gtcaagtcgc tccagggcgg agagatcata ggcgaactga
    18541 tcgacatcac gcttgggaaa tatggagtgt ccatcctgga aactgatgat tcgatggctt
    18601 tcgaaaggac gaagatcata aggggccatt tcatctactc gctggccgaa tctcttgcca
    18661 tagctgtcat cgacgcagat gtcgctcttg taggcgttgc gaatatcaag tacaagaatc
    18721 ccgtatactc cggcacaagg cttatagcga aggccgaagt gaagcagatc aggggaagca
    18781 ggtatattgt ctgggtcagg atctacgaca ggcaggtcga gatgttcaga ggtaaattca
    18841 tactcgtttc ccttgaaaaa ccccaggaaa aatagtattt tattaaatgt agaagaaacc
    18901 gggaaactta cagaaatata cgggagtgaa tcagtttgaa gatattgata gatgctatgg
    18961 gcggagacta tgcaccggat gcggtcgtca acggaagcat cgacgcggtt aaaatgcgtg
    19021 aaggcttcga aatcgcgctt atcggcgaca gggagcagat cgaaaggatc ctcagggcaa
    19081 ggaatttcaa aagcgacagg attgaagtga ttcatgcgaa agaagtgata accaacgatg
    19141 acattccgac acgggcaatc agaagcaaaa agaattcgtc catggtcgtc ggtctcaata
    19201 tgctcaagga caagaaagga gacgcgtttc tttccgccgg gagcaccggg gcgctcctga
    19261 ccggagcgct gttcatactg gggaggatga atggcgtcga caggccggca cttggagcca
    19321 taatccctac caaagccggg aaatgccttc tgatagacgc cgggctgaac acaagatgca
    19381 agcccgtgaa ttacttgcag tttgcgattt ttggttcggc atacatgaaa gggctgttca
    19441 acctggagaa accgagggta ggccttgtca atatgggcac ggaagccagg aaaggcacgg
    19501 agatactcaa gcatgcttac tctctgctga agtcgtccga cctgaatttc gtaggcaata
    19561 tcgaaggcaa ggacatcccg ctgggtaatg tcgatgttgc ggtttgcgac ggatacgtcg
    19621 gaaacgtaat gctgaagttc cttgaaggga ccggatcttt ctttgcaggg cttctgaaag
    19681 gtatgttcac gaagaacatc ttttcgaaaa tatccaccct gttcgtcatg aaagacatga
    19741 aggccttcaa aaagatggta gatccgtcgg aagacggagg agcgcctgtc cttggtgtca
    19801 acggcctcgt tgtgaagagc catggcaact cggatgaaaa aaccatcagg aacgtgatat
    19861 tcaagacatt aagtctcgca aacagctctg tattcgacga gatacgcgac agtgttgaaa
    19921 agatggaggt tgctattgat ggcgaaagga caaaaagaga acgtaagcgt tggtatttta
    19981 ggtataggaa gctgcgtccc agaaaaaatc ttgaccaact cggatcttga aaaaatggtc
    20041 gacacatctg atgaatggat aatccaaagg acagggatct ctgaaagaag gataatggat
    20101 aaaaacactc ctacatatac actggctgta gaagcagcaa aacaggcaat catggattcg
    20161 ggaatatcgc ccgaacagat cggtctgata ctggtagcca ccagtactcc ggattttctc
    20221 accccatcca cttcatgcat cgtccagaag gaaacgggag ccgtcaatgc agctgcattt
    20281 gacataatgg ccgcatgctc aggctttata tacgggctga ctatcgcaaa gcagtttgtt
    20341 gaaaccggtt attatgaata tgttctcctt gtcagcagcg aaggcatgtc aaaggcggtc
    20401 gactggacag acaggaagac atgcgtactt ttcggcgacg gggcaggtgc cgttgtcatc
    20461 ggcaaggtgg aagagggcta cgggatcttg tcgacatacc ttggcgccga cggcgccagc
    20521 ggacatcata taacgatccc atgcctccat ttgccggagg aagaattgga gaaacggaaa
    20581 gattacgaga acaagatcgc gatataccag gacgggcatg aagtattcaa at
//
LOCUS       DAOSCC010000012        20311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1869_length_20311_cov_4.298529, whole genome shotgun sequence.
ACCESSION   DAOSCC010000012 DAOSCC010000000
VERSION     DAOSCC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20311
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_20311_cov_4.298529"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLG72_01500"
     CDS             complement(<1..952)
                     /locus_tag="PLG72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOJ79511.1"
                     /translation="MFAEMIKAFLLIFIAEMGDKTQILAMAFATRFPVRKVLMGIGLG
                     VFLNHGLAVVLGSYLSQVVPINTIQMIAGAAFVGFALWTLKPEAEEEEEKEARMKFGP
                     AATVALAFFMGELGDKTQLTAITLAADARYTFMVLLGTVSGMVATGALGIIIGRKLGD
                     KIPEVGIKLLAASIFMFFGLQKLYQTVPGQFLKAYYVVPFISILALVTAWMVYSMIRR
                     RREGFRSAFINKSKMLHDYYLHMKKDLNNICLGPEYCNRCQGNQCAVGQSKEIVQAAL
                     DSHGRKKLSQETEIKHSEKPFVEEKVLDCLVDTLLLISTIR"
     gene            complement(1676..1768)
                     /locus_tag="PLG72_01505"
                     /pseudo
     CDS             complement(1676..1768)
                     /locus_tag="PLG72_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT9"
     gene            complement(1984..2502)
                     /locus_tag="PLG72_01510"
     CDS             complement(1984..2502)
                     /locus_tag="PLG72_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOJ79512.1"
                     /translation="MERRKYIGRVREDYVKSFSSRVVRVDEKDTAGYASLTEIREVHR
                     PFMAGDVCLYNNGYSELCYIPDRENWTLFAIYDSSGEIVEWYIDITRKNAVDDEGNPY
                     CDDLYLDVALLPDGTALVLDEDELKNALDRGDITRQEYDMAYNVLDEIKSRKILDTGY
                     MESLCSRLLALF"
     gene            complement(2584..3285)
                     /locus_tag="PLG72_01515"
     CDS             complement(2584..3285)
                     /locus_tag="PLG72_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOJ79513.1"
                     /translation="MGKCLLSIDWDYFIYTRDNWGSYIENNRSLIDSWYKRYIQAKTR
                     GEDIRKSFQLSQELDGFWTEISKYFELSNNVKTFVSDSHALSYKITSENNCKAVYLFD
                     SHADLGYGGLSSLNFEVNCSNWLGKLLKDGRIDEANIIYSPYTTEKPDHFKPMNSIYN
                     VRYCGLNDLAGKCTAVSAIHICRSGAWTPPWLDGMFYRFIDALGLPYEIVDCPERKWD
                     PDHISFSNMIDYLMA"
     gene            3989..5767
                     /locus_tag="PLG72_01520"
     CDS             3989..5767
                     /locus_tag="PLG72_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOJ79514.1"
                     /translation="MDITMIVDTIWVVFAALLVFFMNLGFAAVESGFARSKNTVNILS
                     KNFIVFAVSTLGFMLLGWGLMFGGNSPFAGSQHLSVLDSSSIDFYKDTLTSNVPFWGK
                     FFFQLVFCGTAATIVSGAVAERVKYISFILFSFVLTLVIYPIVGHWIWGGGWLANLGF
                     LDFAGDTVVHSVGGWAALAGALILGPRHGKYDKDGKPKAIPGHNMSLAVIGLFVLWLG
                     WFGFNPGSTMSFQNPGDVVHIVVTTNTAAIAAVLTATATSWIFIGKPDLGMTINGCLA
                     GLVGITGSCAFVSVTSSLIIGAIAGVIVVFAVLFFDRVKVDDPVGATSVHLVCGVFGT
                     ICVGLFAQEGVTSLSSVNGLFFGGGLSLLSTEIIGVIAVGAFTFVSSALVWYLLKATV
                     GIRVSLEEEIQGLDIGEHGNSAYPDFAPAVPVLAADNGTTIGAASLAGVHAVSHTETQ
                     KPAYGPVSPEVAIPVINKARPGVKMTKVTIITNQDKFTQLQTALDKIGITGLTVTNVL
                     GYGMQKGHTEYFRGAPVKTRLLPKVQVDIVVCKIPVETVVDTVKKALYTGYAGDGKIF
                     IYDVENVIRIRTGEEGYDALQDEEEK"
     gene            complement(5906..6127)
                     /locus_tag="PLG72_01525"
     CDS             complement(5906..6127)
                     /locus_tag="PLG72_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79515.1"
                     /translation="MIFLLLIYALIFAVTAPGLIKRKEWREFAAFLVMYAIAFGLGLM
                     YVLDIPIPSPMKGLQYLISDKLGLKYPQK"
     gene            complement(6124..7230)
                     /locus_tag="PLG72_01530"
     CDS             complement(6124..7230)
                     /locus_tag="PLG72_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ79516.1"
                     /translation="MSIEKGRISGSQLLFMFAGFVQGSALLVDFTIGITEQQSWMVIL
                     AGLVMMVPFILSYAALAKRFHGKNLVQINDMVYGRFLGKVLSLYYIFFLSMTLSFNIL
                     DVGELYKNFLMPDTPFIFLLAVFAAACAYAVAKGIEVLARVSHLFVITGIFVIVSTFI
                     LLIDQMDFSNFLPLFELPLIRIIQGIHILSAIPYGETLVFFMIMGRLNNTERAAGYAL
                     GGLLIGAMNLLAVSVRNTAVLGRTQSIWTSASFQSIRLIDIGTVLTRMDFLIGIAQTI
                     LIFFKCSLFFYALATALSQLFGLKSYLPLILPLAGIEVILAATVFQSPVDHAVITQNA
                     GIIYSSPLIYVFSPLTLLIAKIRSLPGHERGGGT"
     gene            complement(7232..8419)
                     /locus_tag="PLG72_01535"
     CDS             complement(7232..8419)
                     /locus_tag="PLG72_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ79517.1"
                     /translation="MRKKKGVTIAVIVLCVFMVFALSGCLGGREINDLEIVIGMGVDK
                     DEKTGNILLTAQIVKEQAVGRSSGGSGGGGDEKAFWNVSSTGESIFEAVRQLTHKTGN
                     RLFVSHNQAVIFGNDIASEGLQKYIDFFLRAHEMRPTVLILIAEGRASDVMDAKPDTE
                     KFPAMNIAKLVKTYGYTSHLYKVNMKDFSECLMCSEKASFAPLITVSDNEGSKDVFVP
                     GMAVFKNGKMVGKLGHGEVRGVLWVLGKVKSGVITVPSPDGGGKAILEIIEAKSKAIP
                     EIIDGKIIIHVMVEVELSLSEQTTAENLATVPAFGKIQDASEEVVRKEIMAAFKKTSE
                     LKADIFGFSEMLYKKYSKEWKSMKDNWDDIYPTIELDIDVASRVVTTNLLRNPASPKE
                     GEK"
     gene            complement(8437..10056)
                     /locus_tag="PLG72_01540"
     CDS             complement(8437..10056)
                     /locus_tag="PLG72_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ79518.1"
                     /translation="MFRYIFRKIRSSYLKKENERALREARSGKDNSAPLLPDLDRNLE
                     AVKSILRDSSDVTFHEFNFGHDRRYRAALVFINSMTDKEEIHKNILQPLMFGSLLLSS
                     DADMDFTQIDSIRKNLISITENQKVSLLNDLVDNLLTGAAILLIDGSKEALAVKSRKR
                     ETRSVDEPATEAVVRGPREGFIEDVNVNIALIRRKIKVPDLCVEKLVIGEKSKTDVYI
                     VYVKSVADPKLIEEIRIRLDRIKIDAILESGYIEQFIEDDPWSIFPTVSNTEKPDKVA
                     AKILEGRAAILVDGTPFVLAVPMVFVESFQSVEDYYSRPFLASITRMIRFLSYIISTL
                     GPAIYVALTVFHQELIPTQLLISIAEGRERVPFPAILEASLMIFAFDILREAGVRLPK
                     PVGQTVGIVGAIVLGEASVQAGLISPIMVIVVSSTAIASFAVPAQTDSGTVLRYIYLA
                     LAGIAGGFGVITGLLVTLLHLASLRSFGTPYLWPIAPLNLSGLKDVFVRMPLWMQSKR
                     PKAIAGNGRDLRNQGPELMPSPYKGDNSDSG"
     gene            10415..11926
                     /locus_tag="PLG72_01545"
     CDS             10415..11926
                     /locus_tag="PLG72_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ79519.1"
                     /translation="MRSRKKLEKIMEEALSIRCNTEQMRSGDLDIKIDTGNFNFLKDL
                     AEDINQINITFNAYINEVTHVLSHLSAGNMTVAFSEDLSYKGDFLPIKNALHKIKHSL
                     NNSFEEISKLSAEIDAMSRQVENGSSFLASNTTEQAAFISDLTSTVYDITDKTMNNAA
                     NAKEAAKAIEAITKETETGRLYMNQMLSSVDKVKSSIDDISHIIGLINEIAEQTKLLA
                     LNAAIEAARAGADGQGFSVVAGEIGKLAQKSSEAVRQTKQLINNGIVAAGESAKITKK
                     TAESFESINSSIEGAAGLCNEIAELSNTQAENLRETSAIVANISEAVQGIAAYAQENS
                     AGAMNLSGISAQLKKVLQRFRLAGQENAKSTDMDTGEESIRELAGKLTEKLIHATTAQ
                     AADKILENEIRDLEDVECLYVISGNGRQLSHTIMNPRIMVEHDENFKPALPGEDYSSK
                     KYFRQAMKNRKELYISQEYISKATGNLCRTVSYTYQGIDNEYYVLCIDLLCRF"
     gene            complement(11995..13740)
                     /locus_tag="PLG72_01550"
     CDS             complement(11995..13740)
                     /locus_tag="PLG72_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006777223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79520.1"
                     /translation="MNMDNGSVLKRILRFVKPYIHYLALSLVTAVIYVVSTLYTPVLI
                     GKGVDLILGKGDVDFHGLAPVLMRLGAAIAVTVISQWLLGLCNNKTAYNTARDIRTKA
                     FERLQTVPLKFIDSSQHGDILSRVTTDVDQISDGLLMGFTQLFTGVITILGTIGFMFS
                     VDFRITAIVVLITPLSLFVASFIAKRTFRMFKLQSETRGELTSLVEEMIANQKVVKAF
                     GYEKEAQARFEEINARLQKHGLSATFFSSTTNPATRFVNGLVYTSAGIAGAMLAIGGR
                     ISVGQLSAVLSYANQYTKPFNEISGVVTELQSAFASARRIFDIIDQEPQTPDDPDAVE
                     LTEADGSVSFRDVEFSYRPDVELIKDLNLSVKPGQRIAIVGPTGSGKTTLINLLMRFY
                     DVDSGEIIVSGHPIKKIRRDSLRSMFGMVLQDTWLKTGTIRENIAYGRPDATFEEIVE
                     AAKAAHAHSFIRRLPRGYETRVSEEGENISQGEKQLLSIARVMLTLPPMLILDEATSS
                     IDTRTEIRIQKAFAKMMEGRTSFIVAHRLSTIREADCILVMNNGKIIEQGTHDELLKK
                     GGFYADLYNSQFDIL"
     gene            complement(13737..15557)
                     /locus_tag="PLG72_01555"
     CDS             complement(13737..15557)
                     /locus_tag="PLG72_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79521.1"
                     /translation="MLKLIKYLKGYIKESVIGPLFKLLEACFELIVPLVMANIIDIGI
                     KNGDKAYILKMGGIMVLLGVLGLVCSLTAQYFAARAALGFGTALRNDLFAHVNSLSLS
                     EIDKIGTSTLITRITGDVSQVQTGVNLVLRLLLRSPFIVAGAVIMSFTIHAELALIFL
                     AVSLLISLVIYLIMTRSVPHYKSAQSKLDRVSLITRENLSGVRVIRAFSRQKDELERF
                     GQAHDDLTGTQLLVGRISALLNPATYLVLNAGIIAILWFGGITVYHGDISQGEVIALI
                     NYMSQILLALLALSNLIISVTRASASAVRINEIFAVKPSMADGGAALPGEASEDQTAG
                     LEVKKTGPGHPKAGSMEAKAGPGAPKVEFRDVSFSYNRSDEKALSDITFQAGSGETIG
                     IIGGTGSGKTTLVSLIPRFYDVTGGEVLIDGINVKDYPFSELRKKVAVVPQKAVLFRG
                     TIRQNMQWGKKDATDEEIYKALEIAQAREFVDAKPDGLDTMVMQGGTNLSGGQRQRLT
                     IARALVAGPQVLILDDSSSALDFATEARLRKSLMAQTRDMTVFIVSQRASSIKHADRI
                     IVLDNGMVAGIGKHDELLETCEVYREICLSQLSEDEVKRK"
     gene            complement(15754..16656)
                     /locus_tag="PLG72_01560"
     CDS             complement(15754..16656)
                     /locus_tag="PLG72_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ79522.1"
                     /translation="MDSSILRDCTLCPRECHVDRTAGKKGYCRATDELAVARAALHMW
                     EEPCISGEEGSGTVFFSGCPLGCIYCQNHNISRGLAGKAITTERLAEIFLELQGKGAN
                     NINLVTPSHYVPQITEALDISRRKGLTIPIVYNCSGYEKVETLKLLEGYVDIYLPDLK
                     YISYEPAAKYSNCRDYFPVAAKAIEEMVRQAEEAKFDERGIMQKGVIVRHLALPGYLE
                     DSKRIIKYLYETYGNRIYISIMNQYTPIIMSDVYPELNRRITEEEYKELVDYAVELGV
                     ENGFIQEGETALESFIPEFNGEGV"
     gene            complement(16671..18107)
                     /locus_tag="PLG72_01565"
     CDS             complement(16671..18107)
                     /locus_tag="PLG72_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ79523.1"
                     /translation="MKQTASTGIFSKIRNTFIGDRRFYAAVFSLLLPVIVQNAISNFV
                     NLLDNIMIGQVGTPQMSGVAIANQLIFVFFLAVFGSLSGAGIFGAQFYGAGDHDGVRY
                     TMRFKLLTAVVISAAAITIFLACGDQLISLYLTGEGDDAERTAILVYSRNYLNIMLWG
                     LLPFAITQSYAGTLRETGETMIPMTASLAGVITNLCLNYVLIYGKLGFPALGVEGAAI
                     ATVISRFVELAIIVVYVHRHTGRFLFVEGLYRSMRIPGDLAVDIIKKGTPLLANELLW
                     SLGMTTLTQIFSTRGLNVVAGLNIASTITNLFNVVVFSMGTAVAVMVGQALGAGDIPG
                     AKRTTWRLIFLSICISIVFGGALAGLSPFIPYLYNTTGEVRMMATRFMMTSAMYMGFN
                     AVCHSSYFAIRSGGKTFITFLFDSVYTWLILVPYTYALTHFTALDILVLYPVCYLADI
                     IKCIIGIAIVKSGYWARNMVSDNSPKDS"
     gene            complement(18194..18496)
                     /locus_tag="PLG72_01570"
     CDS             complement(18194..18496)
                     /locus_tag="PLG72_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79524.1"
                     /translation="MDIPVKTERGAVLNVPVLKDSPWVFELEVKQSIPLDDGEVYLCK
                     IRNTLAAKELGDKSISADERLRMAAPVLWIGEGKYYTLNYSSLGRTGDWKDLSGKK"
     gene            complement(18574..20307)
                     /locus_tag="PLG72_01575"
     CDS             complement(18574..20307)
                     /locus_tag="PLG72_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOJ79525.1"
                     /translation="MITGVALFLFGMTLMGEGLKQVAGNKLEIVLFKLTGSPLKGLLF
                     GAGVTTVIQSSSATSVMVVGFVNSGIMKVRQAIPIVLGAIFGTSITGWVICLSGLNAG
                     SGWLALFSAGTLTCATALVGIYFRMFSKNRAKNHIGNVLLGFAVLMCGMSVMSGAVAP
                     LKDNELFTGIITEFSNPILGILVGIFFTSILQSASAAVGILQALAMTGTIRFDTALPL
                     IMGIAIGAAVPVLLSAIGATTDGKRTALAYLTSNTIGVILGAAVFYGLDAFLRFPFMN
                     ETMTMTSIAVLNSVYRFLEVAVLFPLIRQLEALSRWLVRPGSEEGLDELPLKPLEERF
                     LAYPSLAVEQCRVAINDMAAKAKENLFLSFELLHSFSQESYERVQLIENTVDRYEDRL
                     GTYLLKITSRELNEDQNESTGKFLHTIADFERISDHAVNLAEAAREIYEKEIVFSQDA
                     EHELFVIENAVKEIVGTAFDSFLSNDLELAAKIEPLEELIDNLCDELKLHHVQRLKNG
                     VCSISSGFVFNDILNDYERIADHCSNIAVAMIALESDSFDTHAYLDSVKRLKTETYAR
                     YFEAYSRKYAI"
BASE COUNT         5147 a         5269 c         4687 g         5208 t
ORIGIN      
        1 cccggatcgt actgataagc aatagagtat ccacaaggca atccaaaact ttctcttcca
       61 cgaaaggttt ttcactatgc tttatctcag tctcctgcga taattttttc cgtccatggc
      121 tatccagcgc cgcctgtacg atctctttcg actgccctac ggcacactgg ttcccctggc
      181 atctgttaca atactccggg cccaggcaga tgttgttcag atctttcttc atatgcaggt
      241 aataatcatg aagcattttt gatttgttga taaatgcaga cctgaatcct tcacgtcttc
      301 tgcggatcat tgaatatacc atccaggcgg ttaccagtgc caatatggaa atgaagggaa
      361 cgacataata ggcttttaaa aactggccag gtactgtctg atacagcttc tgaagcccga
      421 aaaacatgaa aatggatgct gccagcagct tgatgcctac ttccgggatc ttgtcgccca
      481 gcttcctgcc gatgattatc ccgagggctc cggtggctac cattcctgaa acagttccca
      541 acagtaccat gaacgtatac ctggcatccg ccgccagggt tattgccgtc agttgcgttt
      601 tatcaccaag ctcacccatg aagaatgcaa gtgcgactgt tgcggccggt ccaaatttca
      661 ttcgtgcttc cttctcttct tcttcctctg cctcgggctt cagtgtccag agtgcaaacc
      721 cgacgaacgc tgccccggcg atcatctgga tagtattgat cgggaccacc tgggacaggt
      781 aacttcccaa aactactgca aggccatggt tcagaaatac tcccaaccct atccccatca
      841 gcacttttct tacagggaac cgggtggcaa acgccatcgc gagtatttgg gtcttgtccc
      901 ccatttctgc gataaatatc agcagaaatg ctttgatcat ctcagcaaac ataccagttc
      961 tcctccgaca caaaatccac tgaaaacagc acggcgccca ccggcggctt aagtccgctg
     1021 aacagtctgc tgataacagc acgacgccca ccggcggttt aagtcagctg aacagcctgc
     1081 aactaaaaaa gactttcaga ttatcaggcg cattctgata atctaaaagt ctcgctttcc
     1141 ttaacatacg ttaaagagat aagaccagga tcgctccagt atgttgatct tatcccggtt
     1201 gcccgaagct actccctttt agagctaatg taattttatc agcacatgat atttttgtca
     1261 aacatcgctg ccggcgctct tggttccctt gttctgaagc attttacact gtgtgccggg
     1321 gcccggcgtc tcaatggctg cgctgcctgt tctcttgggt cggcacacct tcctgcatag
     1381 ggctgaatgt tcacttgatc gatgccccct taatgtcttc gtttctctcg aagcatttcc
     1441 gcttcactta cagtatttaa ctgtaccatt tttccgtttc tgggcggcaa gtaacgttgg
     1501 tcctgaaaaa atcccactcc agttgtacag aattttcgtt tatgggcgct taaatgtcat
     1561 attttcccat ccttactata cataatgtga aatagcgcac tttatgtcaa gtaactcggt
     1621 ttaaaataca tgcttcagga gtttttagtg cccagaaacg aaaattttgc acacacaacc
     1681 gggctaacag gataaccaga tccagatttt cggtttgccc ggggatctcg ccgttttcga
     1741 actgctctct cagccattcg ccggtcattc gcgatgatgc ggaatagtcc acgcataacg
     1801 tgtcatatag gattcaaaaa ccttcaccat gcattcaggc aggagaaccg cctccatgtc
     1861 cgggcgaaag gctcaagtcc aggcatggga actgcatctg tgtccgggcg aaaggctcgg
     1921 gttcaggcac gagaaccgcc tccgtgtccg gaaaaaccat ccctatgtct gaagcatcca
     1981 cgtttaaaac aaagccagca gccgcgaaca cagcgattcc atgtaccctg tatccaatat
     2041 ctttctactc tttatttcat caaggacgtt atacgccatg tcatactcct gcctggttat
     2101 atctcccctg tcgagagcat ttttcagttc gtcctcgtca aggaccaggg ccgtaccgtc
     2161 aggcagaagc gcaacatcaa ggtacagatc gtcgcagtac ggattgcctt catcatcaac
     2221 ggcatttttc ctggttatat cgatgtacca ctccacgatc tccccgctgc tgtcataaat
     2281 ggcaaaaaga gtccagttct ctctgtcagg gatgtagcac aattcactgt agccattgtt
     2341 gtagaggcat acatctccgg ccatgaacgg ccggtgtact tccctgatct ccgtcagcga
     2401 cgcatagcct gccgtatctt tttcatcaac cctgaccact cttgatgaaa aagatttaac
     2461 gtagtcttcc ctcacacgtc cgatatactt tcgcctttcc acgagctatc acgctccgtt
     2521 tggtttttgt aattataaca tatcctgcat ttaaaccaaa ccatatctat tttttctctg
     2581 cggtcatgcc atcaggtaat cgatcatgtt ggaaaaactg atgtgatccg gatcccattt
     2641 cctttccggg caatccacga tctcatatgg cagccccaaa gcgtcaataa atcgatagaa
     2701 cataccatcc agccacggag gagtccaggc gccggacctg catatatgga tcgccgacac
     2761 cgcggtgcat ttaccggcca aatcattcaa accacagtac ctgacgttgt aaatgctgtt
     2821 catcggtttg aaatgatccg gcttctcagt cgtatacggg ctgtagatta tgttggcttc
     2881 atcgatccgg ccatctttca ggagtttgcc gagccagttg gaacagttga cttcaaagtt
     2941 gagggatgaa agcccgccat aaccaagatc ggcatgtgaa tcaaacaggt ataccgcttt
     3001 gcagttgttt tccgatgtta ttttataaga aagtgcatga gaatccgaaa cgaaggtttt
     3061 tacattgttt gagagctcaa agtatttact gatttcagtc cagaaaccgt ccagttcctg
     3121 ggaaagctga aatgatttcc ggatatcttc tccccgggtt ttggcctgaa tgtacctctt
     3181 gtaccaggaa tctatcaggc tcctgttatt ttctatgtac gaaccccaat tgtcacgggt
     3241 gtatatgaaa tagtcccaat ctatcgataa caggcacttt cccatacctt aaccccctcc
     3301 tgaccgtggc tcaaacgtta aaatatgagt gctctggtat ctgcctaaat atcctgcctg
     3361 aaacaaagtc ttcgcggtta tttaagaaag atcaggaccc cggtttatcc gaacgggctg
     3421 tgatattcaa aagtatgtca tgaacagcgt cctccctgta ccagtatgtc atgcattatt
     3481 ttaccacaca ggcgttcgtt tgtgaatcag cctttcctgg cttttgattt accgtattcc
     3541 tttttggacc cgatcacgct catacccgag taccgaacgc tcccaggtcc gggcggacag
     3601 caccggttgt ctgttgggca atttcaataa ttcatgggtg ctttggacga ctttaattta
     3661 tcatttttcc ttattgacaa tcgaacaggt tttggatatc atgtgttatt atagcaaagg
     3721 cgctgagggc gaaagtccgc agcgcctttt gttatatttg ggtgagatgg taagggcaat
     3781 tcttcgggat atgccgggca ataatgaaac gcggccattc agccggaact acgccaggca
     3841 tccggaagac tcgaagaatt gcagggaagg cgttccggaa ccgcccgaag cgaaaccatt
     3901 tataactaac ttaatatgag tacgcacaaa ggcgtgtatt ttaccgggtt aaaatacacg
     3961 cctcaatatt tttaaggggg aatagatcat ggatatcaca atgatagttg ataccatatg
     4021 ggtagtgttt gcggctcttt tggtattctt tatgaacctt ggcttcgctg cggtagaatc
     4081 cggatttgcc agatcgaaga atacggtcaa catcctgtcg aaaaacttca tcgttttcgc
     4141 cgtatccacg cttggcttca tgctcctggg ctggggcctg atgttcggcg gaaacagccc
     4201 gtttgcagga tcgcagcatc tgtctgtcct ggacagttcc agtatcgatt tctataagga
     4261 caccctgaca tcgaacgtgc ccttctgggg taagttcttc ttccagttgg tcttctgcgg
     4321 gacggcagct accatagtat ccggtgcggt cgcagagcgc gtcaagtaca tttcatttat
     4381 cctgttctct tttgtactga cgcttgtcat ttacccgatc gtcggccact ggatatgggg
     4441 cggcggctgg cttgccaacc tcggtttcct tgatttcgca ggcgatacgg tcgtccactc
     4501 ggtcggcggc tgggcagctt tggccggtgc tctcatactc gggccccgtc acgggaaata
     4561 tgacaaagac ggtaagccta aagcgatccc gggccacaac atgtcactgg cagtgatagg
     4621 cctcttcgtc ctgtggcttg gctggttcgg cttcaacccg gggtctacca tgagcttcca
     4681 gaacccggga gacgtcgtgc atatcgttgt gactaccaat actgccgcca ttgcggctgt
     4741 actcactgca accgctactt catggatctt cattggcaaa cctgacctcg gcatgaccat
     4801 caacggctgc ctggcagggc ttgtcggcat caccggaagc tgcgcatttg tcagcgttac
     4861 aagttcgctg atcatcggcg ccatcgcagg tgtgatcgta gtgttcgcag tactcttctt
     4921 tgaccgcgtg aaagtcgacg atcctgtcgg cgccacttcc gtacacctgg tatgcggtgt
     4981 tttcggcacg atctgcgtcg gactcttcgc ccaggaaggc gtaacgagcc tttcttcggt
     5041 caacggcctg tttttcgggg gcggcctgtc gctcctctcg acagagatca tcggtgtaat
     5101 tgcagtcggc gcgttcacgt ttgtctcgtc cgcgttagtc tggtaccttc tgaaggcaac
     5161 ggtcggtatc cgtgtcagcc ttgaagagga aatccagggc ctcgatatcg gcgagcacgg
     5221 gaactccgct tatcctgatt tcgctcccgc agtacctgtt ttagctgctg ataacggcac
     5281 cacgatcggg gcggcaagcc tggcaggcgt gcatgcagta agccacactg aaacacagaa
     5341 gcccgcttac ggcccggtat cgcctgaggt tgcgatccct gtgatcaaca aggcacgtcc
     5401 gggagtcaaa atgacaaagg tcacgatcat aacgaaccag gataagttta ctcaactcca
     5461 gactgccctt gacaagatcg gcatcaccgg acttacggta acgaacgtcc ttggatatgg
     5521 catgcagaaa ggccatacgg aatatttccg cggtgctccg gtgaaaacaa ggcttcttcc
     5581 gaaggtccag gtcgacatcg tcgtgtgcaa gatacccgtc gaaaccgttg tcgatacggt
     5641 gaagaaagcc ctgtatacag gatatgccgg cgacggcaag atcttcattt acgatgttga
     5701 aaacgttata aggatcagaa caggtgaaga aggatacgat gcattgcagg atgaagaaga
     5761 aaagtaaacc ctcagggagc atgccaggct tgtcctgcat gctcccgcct cctgtcataa
     5821 gcaaggaaag gggacactcc ttccccagag gaataccact tgtaaaggag tgtcccctta
     5881 cgttgctccg gaatgtcccc tgctatcatt tctgcggata tttcaggcca agcttgtcag
     5941 atataaggta ctgcaggcct ttcatcggac ttggtatagg tatgtcgaga acgtacatca
     6001 ggcccaggcc gaaagcaatg gcatacatga caagaaaagc tgcaaactcc ctccactcct
     6061 tcctttttat aaggcccgga gcagtgactg caaagatcag cgcataaatc aataaaagaa
     6121 atatcatgtc cccccacccc tttcatgccc gggtaagctg cggatcttcg cgatcagtaa
     6181 cgtcaggggg gaaaaaacat atataagcgg ggaagaatat ataatacctg cattctgggt
     6241 gatcaccgca tgatctacag gcgactggaa aacagtagcg gcaaggatca cttcgatgcc
     6301 ggccaaaggc aatatcagcg gtaaatatga cttcaggccg aataattgtg aaagcgctgt
     6361 tgccagcgca taaaagaaaa gactgcattt gaaaaaaatc aatatcgtct gtgcaatgcc
     6421 tatcaaaaaa tccatcctcg tcaacacagt accgatatcg atcaggcgta tggactgaaa
     6481 cgacgcggaa gtccaaatag attgcgttct gcccaaaacc gcagtattcc tgactgacac
     6541 ggcaagaagg ttcatcgcgc cgataagcaa tccgcccagg gcgtatccgg ccgcacgctc
     6601 agtattgtta aggcgtccca tgatcataaa gaaaacaagc gtttcaccat aaggaatggc
     6661 cgataatata tggataccct ggattatcct gataagcggc agttcaaaaa gcggcaaaaa
     6721 attggagaaa tccatctggt cgatcagaag gatgaatgtg ctgactatta caaatatccc
     6781 tgtaattaca aacaaatggc ttaccctggc caggacttct atgccttttg caacggcata
     6841 tgcacaggct gcggcaaata ccgccagcag gaaaataaaa ggtgtgtccg gcattaaaaa
     6901 atttttatac agttcaccta catcaagaat attaaaggac aaagtcatag ataaaaagaa
     6961 aatgtaatat agcgataaaa ctttgcctaa aaaacggcca tacaccatat cattgatctg
     7021 gaccaggttc tttccgtgga acctttttgc aagcgctgcg taggaaagga taaaagggac
     7081 catcataacc aaccctgcca atataaccat ccatgattgc tgctcagtta ttcctatcgt
     7141 aaaatccacc agcaaagcag aaccctggac aaaaccggca aacatgaaaa gtaactggct
     7201 gccgctgatt ctcccttttt caatgctcat atcatttctc tccttccttt ggggatgccg
     7261 ggtttctcag caagttggtc gttactaccc tcgacgctac atcgatgtca agttcaattg
     7321 tcggatatat atcatcccag ttatctttca tacttttcca ctctttgctg tattttttat
     7381 acagcatttc gctaaagcca aaaatatcag cctttagttc actggttttt ttaaaggctg
     7441 ccatgatttc tttgcggaca acttcttccg aagcatcctg tatcttgcca aatgccggaa
     7501 cagtagccag gttttcggca gtggtctgct cagagaggct cagttccact tctaccatta
     7561 catgtattat tatcttgccg tctattatct ccggtattgc tttactcttt gcttctatga
     7621 tttccaaaat ggccttgcct ccgccatcag gcgaaggaac cgttattacg ccgcttttca
     7681 ctttgccgag cacccacaga acgccccgga cttcaccatg gcccagcttg ccgaccattt
     7741 ttccattttt gaagacagcc ataccaggaa caaaaacatc cttgcttccc tcattgtccg
     7801 aaacagtgat caggggcgcg aacgaagcct tttctgaaca catcaggcat tcggaaaaat
     7861 ccttcatatt aactttatac aaatgggatg tatatccata ggttttgacc agctttgcta
     7921 tattcatggc aggaaacttc tcagtatccg gtttggcatc cataacatca gaagcccggc
     7981 cttcggcaat gaggatcaac acggtaggcc tcatctcatg cgcgcgtaaa aagaagtcaa
     8041 tatatttttg caggccttct gaagcaatat cattaccaaa aatcactgcc tggttgtgtg
     8101 agacgaaaag cctgtttccc gttttgtgag tgagctgcct taccgcctca aaaatcgatt
     8161 ccccagtgct tgagacattc cagaatgcct tttcgtcacc accgccgccg ctgcctcccg
     8221 acgatcttcc cacagcctgt tccttaacta tctgcgccgt aagcaatatg tttcctgttt
     8281 tctcatcctt atcgactccc ataccaataa cgatttccag gtcgtttatt tctcttccgc
     8341 ccaggcagcc ggacagggca aacaccataa agacgcataa tactattact gcaattgtca
     8401 cacccttttt tttcctcatt ggcagccact ctccatttat ccggaatcgc tgttgtctcc
     8461 cttatacggc gacggcatca gttccgggcc ctggttccgt aaatcgcggc cgttcccggc
     8521 aatagctttt ggacgttttg actgcatcca cagtggcatc cttacgaaaa catccttcag
     8581 ccctgaaagg ttcaatggtg cgattggcca taaatatggc gttccaaaag atcttaagga
     8641 cgccagatgc agcaatgtca ccaaaaggcc tgtaatcacg ccgaaaccgc cggcaatccc
     8701 ggcaagtgca aggtatatgt accgcaatac ggtcccgctg tcagtctgcg ccggaacggc
     8761 aaagcttgca atggctgtcg aggatacaac gataaccatg atcggactta taagtccggc
     8821 ctgaacggat gcctcgccca gtacgatggc acctacgata ccgactgtct gacctaccgg
     8881 cttcggcagg cgtactcctg cttccctcaa aatgtcaaaa gcaaatatca tcagcgaggc
     8941 ttccaatatt gccggaaacg gtacacgttc acgcccttct gctatggata tcagcaactg
     9001 tgtgggtatc agttcctggt ggaaaacggt caatgccaca tatattgcgg gccctaacgt
     9061 gctgatgata tatgacagaa accttatcat ccgtgtaatg cttgcaagaa acggccgtga
     9121 ataatagtcc tccacgcttt ggaagctttc tacaaacacc atgggtaccg caaggacaaa
     9181 aggcgtaccg tcgacaagga tggcagcacg gccttcgagt atttttgcag cgaccttgtc
     9241 aggtttttca gtatttgaaa cagtggggaa aatggaccag ggatcatctt ctataaactg
     9301 ctcgatatat cctgattcca ggatcgcgtc tatttttatc ctgtccaggc ggatcctgat
     9361 ttcttctatc agtttcggat cagccacact tttcacatat acgatgtaca cgtctgtttt
     9421 tgacttttca ccgattacaa gtttttccac acaaaggtca ggaactttga tttttctgcg
     9481 gataagggca atgtttacat ttacgtcctc aataaaacct tccctgggtc cgcgcactac
     9541 agcctcggtt gccggttcat ctacacttcg tgtttccctc ttcctggact tgaccgccaa
     9601 agcctccttt gagccatcta tcagcagaat agcagcccct gtcagaagat tgtcaacaag
     9661 atcgttcagc agtgacactt tctggttctc agttatcgag atcaggtttt tcctgatgct
     9721 gtcgatttgt gtgaagtcca tatccgcatc gctgctgagc agcaatgagc caaacattaa
     9781 aggctgcagg atgtttttat ggatttcttc cttgtctgtc atgctgttga taaatacaag
     9841 agccgccctg tatcttctgt cgtgtccgaa gttgaactcg tggaatgtca catcgctgct
     9901 gtcgcgcaaa atgcttttga cagcttccag gttcctgtcc aaatctggta ataacggcgc
     9961 gctgttgtcc ttgcctgatc tggcttccct gagcgcacgt tcattttctt tttttaaata
    10021 cgaactgcgg atcttcctga aaatatatct gaacataagc aattcttccc aaccggtttt
    10081 tgcttatttt ctgcacatac aggcaattta ttcatgtact gagaaatacc cggcactgca
    10141 tatatgagcc ggaaacgggc tgcagcattt atcattgcca cgttcttgac aattttcaat
    10201 ttgtaatata tagtaattaa caagcaacga ggcgaaaacc ttgttgcttt ttttgattaa
    10261 tgccggtcaa gcgccgcttt caaagggcat tggcagatat atttttacgg gaaaacaggt
    10321 tccgtgccgc tgaaaaaccg gtattgcaat aactgctgat tcccaggcgg acgatgaaaa
    10381 accggaaaga ttggtgcaaa aggaggcgac ggggatgaga agccggaaaa aactggagaa
    10441 gataatggaa gaggcactca gtataagatg caatacggag caaatgcgca gcggcgatct
    10501 ggatataaaa atcgatacgg gcaattttaa ttttcttaaa gatctggcag aggatatcaa
    10561 tcagataaac attaccttta atgcttacat aaacgaggta actcatgttc tgtcccattt
    10621 gtcggccggc aatatgacgg tagccttttc agaggattta agttataagg gagatttttt
    10681 gccaataaaa aatgctctcc ataaaatcaa acattctctg aacaattcgt tcgaagagat
    10741 ctctaagctg tccgcagaaa tagacgccat gagcagacag gttgaaaacg gttcttcatt
    10801 tttggcttca aatacgacgg aacaggcagc ttttatttcc gacctgacat caaccgttta
    10861 tgacattacg gacaagacaa tgaacaatgc ggcaaacgcc aaagaagcag cgaaagcaat
    10921 tgaagcgata acgaaagaaa ccgaaacagg caggctctat atgaaccaga tgctgtcatc
    10981 cgttgataaa gtaaaatcct ccatcgatga tatatcccat atcattggat taataaatga
    11041 aattgcagag caaaccaagt tgctggctct gaacgcagca atcgaagctg cgagggccgg
    11101 tgcggacggg caggggtttt cggtggttgc aggtgaaata ggcaaactcg cacagaaaag
    11161 cagcgaagcc gtaaggcaaa ctaaacagtt aatcaacaat gggatagttg cagccggtga
    11221 aagtgcaaaa atcacgaaaa aaacagcaga aagttttgaa agtataaaca gttcgatcga
    11281 aggagccgcc ggtttatgca atgaaatagc ggagctttcc aatacccagg cagaaaatct
    11341 cagggaaacg tcggctatcg tcgccaacat atcggaagct gtacagggta ttgccgcata
    11401 tgcacaggaa aacagcgccg gagcaatgaa cctgtcaggt atatctgctc aattgaagaa
    11461 ggtacttcag cgtttcaggc tggcagggca ggaaaatgca aagtcaactg atatggatac
    11521 aggggaggaa tccatacggg aactggccgg caaattaacg gaaaagctga tccatgcaac
    11581 taccgcccag gctgcagata agatactgga aaatgagatc cgggatctgg aggatgttga
    11641 gtgcctgtat gtcatcagcg ggaacggaag gcagttgagc catacgatca tgaatcccag
    11701 gatcatggtg gagcatgatg aaaacttcaa accggcactg cccggcgagg actacagttc
    11761 aaagaagtat ttccgtcagg ccatgaaaaa caggaaggag ctgtatatat cccaggaata
    11821 catatcaaag gcgaccggaa atctatgcag gacggtttca tatacatatc agggtatcga
    11881 caatgaatat tatgtgctgt gcatcgacct gctgtgcagg ttctagacca acgccggaat
    11941 gcagccgatt ccctgatgtt ttttttgcag gaggtcgtcc ttatggcagc aatactatag
    12001 aatatcgaac tggctgttgt acaggtcggc ataaaacccg ccctttttca gaagttcatc
    12061 atgggtcccc tgttctatga tcttaccgtt gttcatgacc aggatgcagt ccgcttccct
    12121 gatggtagaa agcctgtggg cgacgataaa gctcgtacgg ccttccatca ttttcgcaaa
    12181 ggccttctgg atcctgatct cggtcctcgt gtctatggag gaagtagctt cgtcaaggat
    12241 cagcatcggc gggagcgtga gcatgactct tgcaatggaa agcagctgtt tttctccctg
    12301 ggagatgttt tctccttcct cggacacccg ggtctcatat ccccggggaa gacgcctgat
    12361 gaagctgtgg gcatgcgccg cctttgccgc ttcaacgatt tcttcaaacg ttgcgtcagg
    12421 cctgccgtat gcaatatttt cccgtatggt cccagttttc agccatgtat cctggagcac
    12481 catcccaaac attgaacgca ggctgtcccg cctgattttc tttatgggat ggccgctgac
    12541 tatgatttcc ccggaatcca cgtcgtaaaa tctcataagc aggttgatca gggtcgtttt
    12601 cccgcttccg gtcggcccaa cgatggcgat cctctgcccg ggctttaccg aaagattaag
    12661 gtctttgatc aactcgacat cagggcggta ggagaactcc acatcccgga aactgacact
    12721 gccgtcggcc tcagtcagtt ctactgcatc cgggtcgtca ggcgtctggg gttcctggtc
    12781 tatgatatcg aatatcctcc ttgcggacgc gaaggcgctt tgaagctcgg tcaccacgcc
    12841 cgatatctcg ttgaaaggtt tcgtatactg gttcgcatag cttaaaacgg ctgaaagctg
    12901 cccgactgaa atcctgcctc cgattgcaag catggcgcct gctatacccg ctgaagtgta
    12961 caccagtccg ttcacgaacc gagtagcagg attcgtcgta gaggagaaaa aggtcgcact
    13021 cagtccatgc ttctgcaggc gggcattgat ctcctcaaac cgtgcctggg cttccttttc
    13081 atagccgaat gccttgacga ccttttgatt ggcgatcatc tcctctacaa gcgacgtcag
    13141 ttctcccctt gtttccgatt gcagtttgaa catccggaaa gtgcgcttcg cgataaaact
    13201 tgcgacaaat aaagaaaggg gcgttatgag gaccactatg gccgtgatcc tgaaatcgac
    13261 agaaaacata aagccgatgg tgccaaggat agtgatcact ccggtaaaaa gctgggtgaa
    13321 tcccatcagc aggccgtctg aaatctggtc cacatccgtg gtcaccctgc tcaggatgtc
    13381 tccatgctgg ctggaatcga taaatttaag gggaacggtc tgcagccgct caaaagcttt
    13441 agtccgtata tccctggcag tattgtaagc cgtcttgttg ttgcaaagtc ccaaaagcca
    13501 ttgggaaatc accgtgacgg caatggcggc gcccaaacgc atcaaaacgg gagcaaggcc
    13561 gtgaaagtcc acatctccct ttcccaggat aagatccact ccctttccta tcaaaacagg
    13621 ggtgtaaagg gtagacacaa catatatgac cgccgtgacc agcgacaaag caaggtaatg
    13681 gatatatggc tttacgaatc gaagtatgcg tttgagtacg ctgccattat ccatattcat
    13741 tttctcttca cctcgtcttc cgaaagctgc gacaggcaga tctccctgta aacctcgcag
    13801 gtctcgagca gttcatcgtg ctttccgatc ccggccacca ttccgttatc aagtacgatg
    13861 atcctgtcag catgctttat cgatgaagct ctttgagaaa ctataaatac agtcatatcc
    13921 cgggtttggg ccataaggga tttcctcagc cttgcttcag tggcaaaatc gagcgcggac
    13981 gagctgtcat ccaggatcag tacctggggt ccggccacga gtgccctcgc aatggtcagc
    14041 ctttgcctct gtcctccgga taggttcgtc cctccctgca tgaccatcgt gtccaggccg
    14101 tcaggttttg catctacaaa ctccctggcc tgggcgatct ccaaagcttt atatatctcc
    14161 tcgtcggtcg cgtccttttt gccccattgc atgttctgcc tgatggtccc cctgaagagg
    14221 accgctttct ggggcacgac ggccaccttt ttcctgagct cggagaacgg gtaatccttc
    14281 acgtttatcc cgtcgatgag gacttctccg ccggtgacat catagaaccg tgggatcagg
    14341 ctgaccagcg tcgttttgcc cgagcccgtg ccgcctatga tccctatggt ttcaccgctt
    14401 cccgcctgga acgtgatatc ggaaagagcc ttttcatcgg atcggttata agaaaacgaa
    14461 acatctctga attcaacctt tggggcgccg ggtccggcct tcgcttccat ggatccggcc
    14521 tttggatgcc cgggtccagt ctttttgact tccaacccgg ctgtttgatc ctcggacgcc
    14581 tcgccgggca gtgcggcgcc gccatccgcc atagaaggct ttacggcaaa gatctcattg
    14641 atcctgacag ctgaagccga tgctctcgtg acagaaatga tcaggttcga cagggcaagc
    14701 agcgccagga ggatctgtga catgtaattg ataagcgcaa tgacctcgcc ctgggaaatg
    14761 tctccgtgat atacggtaat gccgccaaac cacaggatcg cgattattcc ggcattcaaa
    14821 accaggtagg tcgccgggtt cagcaaagcc gatatccttc ccaccagaag ctgggtcccg
    14881 gtcagatcat catgggcctg cccaaaccgt tccaactcat ctttctgcct tgaaaaagca
    14941 cggatcaccc tcacgcctga aaggttctcc cttgtaatga gggaaaccct gtcaagtttg
    15001 ctttgggcgc ttttataatg cgggacggaa cgtgtcatga tcagatatat aaccagggat
    15061 attagcagtg acacggccag gaaaataagg gccagttccg catggatcgt gaacgacatg
    15121 atgacagccc cggcaacgat aaacggggaa cgcagcaaaa gtcgcaatac caggttgacg
    15181 cctgtctgca cctggctgac gtctccggtg atcctcgtga tcagcgtcga tgtcccaatt
    15241 ttatcgattt cggagagcga aaggctattt acatgggcga acaggtcatt cctcagggca
    15301 gtgccaaacc ccagcgccgc cctggcggca aaatactggg cagtaagcga gcaaaccagt
    15361 cccaggacgc caagcagcac catgatgccg cccatcttca ggatataggc tttgtcgccg
    15421 ttcttaatgc ctatatctat gatattggcc atcaccaggg gcactatgag ttcgaaacag
    15481 gcctccagca gcttgaagag cggcccgatg acactttcct taatataacc tttcagatat
    15541 tttatgagct tcagcataat tcccgtccac ctgtcagcat cttgagcatc cgttcatcat
    15601 tttgccaggg cacaccagca tacggcaata catcttctta aatactttct catatattat
    15661 cacatttttg acaaaaatct cgacctgtct atgtacggct tcatgaaaaa atcacctccc
    15721 gggtatctgt ccggtggtga tccgtgatca tgattatacg ccttccccgt tgaactccgg
    15781 gataaagctt tccaaagccg tttccccttc ctgtatgaag ccgttctcca ctccgagttc
    15841 cacagcgtaa tcaacaagtt ctttatattc ttcctcggtt attctcctgt taagctccgg
    15901 atacacatca ctcattatta tcggggtata ttggttcatt atgcttatat atatcctgtt
    15961 tccataggtt tcataaaggt atttgattat ccttttggag tcctcaagat atccgggcaa
    16021 tgccaggtgc ctcactatca cgcctttctg catgatgccc ctttcatcga atttcgcttc
    16081 ttccgcctgc ctgaccattt cttctatcgc cttagcggca acggggaaat aatccctgca
    16141 gttggagtac ttcgccgccg gctcgtacga tatatatttg agatcaggca ggtatatatc
    16201 gacatatcct tcgagaagtt tcagggtctc gaccttctca tatccgctgc agttatatac
    16261 gatggggatg gtgagccctt ttctgcgtga aatgtccaaa gcctctgtaa tttgcggaac
    16321 ataatggctc ggggtgacca ggttgatatt gttggccccc ttaccctgca gttccaggaa
    16381 tatctccgcc agcctttcag ttgtgatcgc ctttcccgcc aggcctctcg aaatattgtg
    16441 attctggcag tatatgcaac ccagcggaca gccggagaaa aacaccgtac cggagccttc
    16501 ttcgcctgaa atgcacggct cctcccacat gtggagagcc gcccttgcca ccgccagttc
    16561 atcggtagcc ctgcagtatc cctttttacc ggcagtcctg tccacatggc attccctggg
    16621 gcaaagcgtg caatctctca aaattgatga atccatcaat gatccccgtt tcatgaatcc
    16681 ttcggtgagt tgtccgaaac catgttccgt gcccagtacc cggatttcac tatggctata
    16741 ccgattatac actttataat gtcggcaagg tagcaaaccg gataaagcac taaaatatcc
    16801 agggcagtaa aatgtgtgag cgcatatgtg tacgggacaa ggatgagcca ggtgtaaaca
    16861 ctgtcaaaga ggaacgtaat gaacgttttt ccccccgacc ggatagcgaa gtatgagcta
    16921 tgacatacag cattgaaccc catatacatg gcgcttgtca tcataaaacg cgtagccatc
    16981 atgcgcactt cgccggtggt attgtataaa tacggaatga atggcgataa acccgcaagt
    17041 gcgccgccaa acacgatact gatacagatg ctcaggaata tgagcctcca ggtggtccgc
    17101 tttgcccctg gaatatcgcc cgccccgaga gcctgtccga ccatgacggc aactgcggtt
    17161 cccatggaaa atacgacaac attgaacagg ttcgttatgg tgctggctat gttgaggcct
    17221 gcaaccacat ttaatccacg ggtcgagaat atctgagtca aagtggtcat gccaagagac
    17281 cacagcagtt cattcgccag caggggcgtt ccctttttga ttatatcgac cgcaaggtct
    17341 cccgggatcc tcatcgaccg gtaaagccct tcaacaaaca ggaatctgcc ggtatgcctg
    17401 tggacataca caacaataat ggcaagttcc acaaaacgag agataactgt cgcgatggct
    17461 gccccttcga cacccaaggc agggaacccc agctttccgt aaatcaatac gtaattcagg
    17521 cagagatttg tgatcacgcc ggcaaggctt gccgtcatcg gaatcattgt ctcccctgtc
    17581 tcgcgtagcg ttcctgcata cgactgggta atggcaaacg gcaggagccc ccacagcatt
    17641 atattaagat agttcctgct gtacacgagg atggctgttc tctcggcatc gtccccttca
    17701 ccggtcagat acagtgatat caattggtcc ccgcaagcca ggaatatggt tatcgcagcc
    17761 gcagaaatga caacagcggt caggagcttg aaccgcatcg tatagcgcac gccgtcatgg
    17821 tcgcccgcgc cgtaaaattg tgcgccgaat atcccggctc ctgaaaggct gccaaaaacg
    17881 gcaagaaaga atacgaatat aagctggttg gcaatggcca caccagacat ctggggggtg
    17941 ccgacctgcc ctatcatgat gttgtccaga aggttgacga agttcgatat ggcgttctgc
    18001 acgattaccg gcagcagcag ggagaatact gcggcataga acctgcggtc gccgatgaat
    18061 gtattgcgta tcttgctgaa aatgcctgtt gatgctgtct gtttcatata tggccctccg
    18121 tttttgccga tagaagccat tgtatcacaa aaactgcacc atagtccagg caaaacttcc
    18181 ctctgtgttt agtctatttt ttgccggaca ggtctttcca gtcgccggtc cggcctaaag
    18241 acgaatagtt cagtgtataa tacttccctt ctccgatcca taatacggga gcggccatgc
    18301 gcagccgttc atccgcgctt atcgacttat ctccgagctc tttggccgca agtgtattgc
    18361 ggatcttaca gaggtacacc tccccgtcat ccagaggtat ggactgcttt acttcaagct
    18421 caaacaccca cggactgtct ttcagtaccg gaacgttcag tacagccccg cgttcagttt
    18481 tgacaggaat gtccattttc ccgtcatgtt gtcctgtaac ttttcaccat aaccgggaca
    18541 tattactcag gatggattta taattgccag atcttatatc gcgtattttc tcgaatatgc
    18601 ctcgaaatac ctggcgtaag tttctgtttt taggcgtttt acgctgtcaa gatatgcgtg
    18661 cgtgtcaaag gagtccgatt ccagcgcgat catcgccacg gcgatatttg agcagtggtc
    18721 cgctattctc tcataatcgt tcaggatatc gttgaacaca aagccggaac tgatggagca
    18781 tacgccgttt ttaagtctct ggacatgatg gagtttgagt tcgtcgcaca aattgtcgat
    18841 cagctcttcc aaaggttcga tcttggctgc aagttccagg tcgttgctaa gaaaagaatc
    18901 gaatgccgtt cccacgattt ctttgacagc attctcgatt acgaacaatt catgctcagc
    18961 atcctgagaa aagacgatct ctttttcata gatttctctc gcagcttcgg caagattgac
    19021 cgcatggtcc gaaatgcgct caaagtcggc aatggtatgg aggaactttc cggtactctc
    19081 attctgatcc tcattcagtt cgcggctggt aattttcaac aaatacgttc ccagcctgtc
    19141 ttcataccgg tctactgtgt tctctatcag ctgcacacgc tcgtaactct cctgcgagaa
    19201 actgtgaagc agttcgaacg aaaggaagag gttttctttt gccttggctg ccatatcatt
    19261 gatcgctacc ctgcattgct cgacagcaag cgacgggtaa gcaagaaagc gttcctccag
    19321 tggtttcagg ggcaattcat caaggccttc ttcgctgccg ggacggacca gccatctgga
    19381 gagggcctca agttgcctga tcaacgggaa caggacagcg acttctaaaa atcgatatac
    19441 actgttaagt accgcaatgg atgtcatagt cattgtttca ttcataaaag ggaacctgag
    19501 aaaagcgtcc agcccgtaga ataccgccgc gccaaggatc acaccgatgg tgttgctggt
    19561 caggtaagcc aatgcggtac gctttccatc cgtggtcgcg cctattgcgg ataacaggac
    19621 cgggacagca gcgccaatgg caatgcccat tataagagga agcgctgtat caaaccggat
    19681 ggtccccgtc atggccagtg cctgtaaaat ccccacagcg gcggaagcgc tctgcaggat
    19741 gcttgtaaag aaaatgccca ccaggatgcc taatatagga ttggagaatt ccgtaataat
    19801 gccggtgaac agttcgttat cctttaacgg tgccacggcg ccgctcatga cggacatgcc
    19861 gcacatcagg accgcaaacc cgagcagtac gttgccgata tggtttttgg cacggttctt
    19921 cgagaacatg cggaaataga tgccaaccaa cgcagtggcg catgtcagcg tgccggcaga
    19981 gaacagcgca agccaccccg aacctgcatt gagcccggac aggcagatca cccagccggt
    20041 gatgctggtg ccgaaaatcg caccaagtac gatgggaata gcctgtctta ccttcatgat
    20101 gcctgaattt acaaaaccaa cgaccatgac cgacgtcgcc gatgaagact ggatcaccgt
    20161 agtgaccccg gctccgaaca ggagtccttt cagcgggctt cccgtcagtt tgaaaagtac
    20221 gatctccagc ttgtttccag ctacctgttt caatccttct cccatcagcg tcataccgaa
    20281 caggaaaagg gctacaccgg taatcagttc g
//
LOCUS       DAOSCC010000013        19902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1918_length_19902_cov_4.667960, whole genome shotgun sequence.
ACCESSION   DAOSCC010000013 DAOSCC010000000
VERSION     DAOSCC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19902
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_19902_cov_4.667960"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..517
                     /locus_tag="PLG72_01580"
     CDS             101..517
                     /locus_tag="PLG72_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79526.1"
                     /translation="MAYFDIEKVSKEIESYFGSEDECGKIKGAAVRHYTANEMDKVLE
                     PYVGKPEEFISFIEETWGWKITFDRDAGIIIADENKPFCVCPLDDRVKCPKLCNCSEG
                     FAEMMFSKVFGRPVRATVKASVLRGDKSCVYEVKLA"
     gene            complement(578..994)
                     /locus_tag="PLG72_01585"
     CDS             complement(578..994)
                     /locus_tag="PLG72_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ79527.1"
                     /translation="MKSYRKVLYFNTPTRRAYINITQQVQKCIDESGIKEGLVLVNAM
                     NITASVFVNDDEPGLHHDFEVWLEKLAPEKPYSQYEHNGFEDNADAHLKRQIMGREVV
                     AAITNGKLDFGTWEQIFYGEYDGRREKCVLVKIIGE"
     gene            complement(1112..2554)
                     /locus_tag="PLG72_01590"
     CDS             complement(1112..2554)
                     /locus_tag="PLG72_01590"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ79528.1"
                     /translation="MVENNLTMLTDFYELTMANGYLQSGMKDRIAYFDMFFRRVPDDG
                     GFAIMAGLEQFIQYIKQLRFNEEDIEYLRSKNQFSEEFLDYLRNFRFECDVWAIPEGT
                     PIFPYEPIVTVRGPVMQAQFIETMLLLSINHQSLIATKANRIVTAAQGRPVMEFGSRR
                     AQGYNGAVEGARAAYIGGCVGTACTIADREYGIPALGTMAHSWIQMFPSELEAFRAYA
                     RIYPSNCTLLVDTYNVLKSGVPNAIRVFNEEVVPRGYRPKGIRIDSGDITYLSRKARK
                     MLDEAGFKDCAIVASNSLDEYIIRDILSQGACVDSFGVGERLITSRSEPVFGGVYKLS
                     AVEEDGKIIPKIKVSENVEKITNPAFKQVWRLYDRDSGKALADVITLHDEVINDNEPY
                     EIFDPEYTWKRKTVTNFRARKLMVKIFDRGRCVYESPDLRTIRDYCREQVDTLWEEVK
                     RFENPHRYYVDLSQKLWDIRAKLLHEYSMR"
     gene            complement(2603..3124)
                     /locus_tag="PLG72_01595"
     CDS             complement(2603..3124)
                     /locus_tag="PLG72_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79529.1"
                     /translation="MDGISLFELKKDDREKADELAELAREIWTEHYTPLIGPGQVSYM
                     LSKFQSAGSILNDIAANGYRYFIVYDGSKPAGYFAVKPEPDKKALFLSKFYLEKSCRG
                     RGISRIMLDRIMRIARESGLGYIWLTVNKYNETSIKIYQRLGFRIVEDIVTDIGNGFV
                     MDDYKMRLDMDQG"
     gene            complement(3290..5086)
                     /locus_tag="PLG72_01600"
     CDS             complement(3290..5086)
                     /locus_tag="PLG72_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79530.1"
                     /translation="MPINNFREDEELIESLNITIIRRLLTYLKPYIGPVIKTLVLMAA
                     VIAVELFNPYLMKLGIDKFIAEGDWKNLFLLGGAMIIVNIVAVVCSRLRTLIMGRVSN
                     NIILTIRQQLYSHIQKLSFSFFDSRPTGKILSRIIGDVNSLNDVFANSVTSLIPELVK
                     IVSVVIIMMLMNYRLGLVALATLPFMLAAFFFISTRSRKRWQVHRKKSSNLNAFTHED
                     ISGIRVVQSFAAEHETSKTFLQLCHEWRDSFIRAVRINDCFWPTVEMSWGVGTILVFW
                     FGASMIETGNITPGLLVAFTSYVSMFWQPIMNISNFYNQLVTNMSGAERIFEILDIEP
                     DIKDRPDTKPLPEIKGSVSFRNVTFGYDEGQEVLKNVSFDVSPGQTIALVGSTGAGKT
                     TIVNLISRFYEVTSGEVLIDGYNVNDVTLESLRSQIGVMPQDTFLFSATIMENIRYGK
                     LDATDEEVIEAAKAVSAHEFIMKLEKGYQTEVNERGTRLSAGQRQLIAFARALLANPR
                     ILILDEATASIDTHTERLVQQGIRRLLEGRTSFVIAHRLSTIRNADRIMVVEEGGIVE
                     SGTHEELIRQKGQYYNLYTSQFRFLNGEEEAG"
     gene            complement(5086..6822)
                     /locus_tag="PLG72_01605"
     CDS             complement(5086..6822)
                     /locus_tag="PLG72_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79531.1"
                     /translation="MKRLLRFIAKRWFTYFLGFAALVTAIVLDMFNPYYVGQIIDRVI
                     LGGEMPYLRTALLALAVITFGRAVLGYTKEYMFDLSSSRVIVDLRKELFDHIQKLSFA
                     FFDRSNTGELMSRIKEDTDNIMSAICYGFMLFLEQTIYFIVAAVILFSISWKLALVSL
                     VTMPLIEYVVFRLERQVGQQYEKISDQRAVLNMTAQENIAGVRVVKAFGREKHEILKF
                     LKQNEENFRINYDLVKSMANYNPLIEFLSNLVSVLVITVGGYFVIGKEMSLGTLVAFS
                     NYVWMLIWPLRMMGWLTSVLAQCIASVKKIDKLFAEEPDIKNCPEPKLPKKISGHVEF
                     RDVCFDYEGVSVLKNINIDAKPGSTIAIMGMTGAGKTTLINLIGRYYDCTAGSVYFDG
                     VDVREMDLAALRKNVSVVMQDTFLFSDTIEANIKFGAGEVPDKVFMAACRDAMVDEFV
                     SQMPEGYQTVIGERGIGLSGGQKQRISIARALVRDAKVLILDDSTSALDMETEYAIQR
                     AMERRKGFTCFIIAHRVSAVKNADEILYFEDGRIVERGTHDELLRLRGRYYETYREQY
                     RDYVEALEKEVV"
     gene            complement(7356..8408)
                     /locus_tag="PLG72_01610"
     CDS             complement(7356..8408)
                     /locus_tag="PLG72_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOJ79532.1"
                     /translation="MSEKNINTILYFMLVIAVILYVSVNMPEMVCVYLARDTEYVSAG
                     TGLITGDADGHGNCSCSGPERQQADVSDENADEYVPEQSAADADIETSAESGGPGGDT
                     GNGSVDEGVRPETGSSDQREDASSVYEEEKPKETKHNVSPEGWHLYIDLDDKLMYVYK
                     DGELVKKYEVSGGKPSSPSPLGTWRIISKDTWGEGFGGAWLGFNVPWGKYGIHGTTEP
                     WAIGKSNNSRGCIRMRNKEARELYKLVPYNTLVTVVHENRPFYPMRDGDVGSDVLAVE
                     KALKKLGYYHGGEDGIFGSSLKKAVMEFQKDNKLYVTGVINNSTYEKLMKLEKEYDEE
                     QEGLKKERKDGLKKLE"
     gene            8636..11719
                     /locus_tag="PLG72_01615"
     CDS             8636..11719
                     /locus_tag="PLG72_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ79533.1"
                     /translation="MKRLLSKTTTIILMTAMLITLASANACASDRDPAIPASSDGNGS
                     FSNPAASNTLALAAGVTGAVMKSTAVMYANPVLANNSAVNGKTDQSSSVSAKKEMRGL
                     WVATVVNIDYPSKPTTDPEVLKKEAVEILDNAKNTGLNAVFLQVRPTADAFYKSNFFP
                     WSRFLTGTQGKAPDGGFDPLAFWIEEAHARGIELHAWINPFRITKKTASEAKPTVSSL
                     HSSHPARKNPGWVVQYSDGNLYFDPGIPEVRKLVVDGVMELVNNYDIDGIHFDDYFYP
                     GRDFNDSKTYAAYGKGFKNIGDWRRDNINKLVSDVYKAIKSSGRDVRFGISPFGIWAN
                     KKNNSLGSDTNGLETYYDHFADSRKWVKDGIVDYICPQLYWNIGYKVADYSKLLTWWK
                     DVANGTSVDLYIGHAAYKTGDTDKTSPWYGVAEIERQMLLNRNYPEVKGSLFYNYTSL
                     KNSPALRALIRGVYDRIDGKTSSEVQLKVSWPSKDITTSYTQYYLTGASDPSKPLYIN
                     GKPVESRSPKGYFGMLVDLAKGANTFTFTQEGAYVTRTITRSAGSSTAAKMSKAEIIA
                     SSVFPQSQEYRMPGEKITLSCQAPAGAKVTVKIGSKTYNMTTASKSGGLYKATFTYTY
                     TIPSYTGTPRVIDLGAPVYTMSYNGTTKSVTAPAKVGVVMKGAPFYAEVQNEDIDTFE
                     TPNSANGAEYELRKGMVDYVTGMTGSYARLASGLWVRKTSISTYTSKATLSPKVTSAV
                     YTTGKKWDTLKLTYQSSLAATASFDGSKLKVNISAAEWGVIPALPSDAPFSSATFSKN
                     GHVGQYVLTLKSGSNIEGYYIEKVSDGIIVHIKRPVIASPGDKPLEGITIMLDPGHGG
                     SDTGTTGPLGTEYPEKRINLENALMLKSELEKRGAKVLMTRTADVTISLSDRLTASRD
                     AKPDMFLSIHANSMAENVDISKIQGFSTHYKEAIAKPLSQALCNAAAEAGRVNKGVRW
                     ANFYVVRGTWAPSMLIEVGFVPNPTEFEILTDDLEQKYLAMKLADAIVDYFSSKR"
     gene            12057..12434
                     /locus_tag="PLG72_01620"
     CDS             12057..12434
                     /locus_tag="PLG72_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ79534.1"
                     /translation="MKLDFSDEKSIYLQIAESIEDDIIKGVIEEETQVPSTNQMAVIY
                     KINPATAGKGINLLVDKGVLYKRRGIGMFVAAGARKKILEERKNSFYSSYIVTLLEEA
                     KKLGIGTDEIIDMIRKGENTHER"
     gene            12424..13314
                     /locus_tag="PLG72_01625"
     CDS             12424..13314
                     /locus_tag="PLG72_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79535.1"
                     /translation="MSDNILLETRSLTKRYGKVTALNNFSVALESNKIYGLLGRNGAG
                     KTTLLDIITSRRFAGAGDVRLFGQDAKENQKVLPNVCYMPEKNYFIPAMKVFEILETS
                     AMFFDSFDMEYADGLCAKFGLDKRKKYKALSRGYESILRIVVGLASRAPLTIFDEPVL
                     GLDAAVRDLFYRELLEDYTSHPRTFIVSTHLIEESADVFEDVLIINNGELICRHQVAE
                     LREMAFYVTGRPSDVDAAVNGMKVIHSEMVGSVKVSAVFNKINDEMKNRLKTCNVDIS
                     PIPVQKLFIYLTNSNKGVFQ"
     gene            13311..14045
                     /locus_tag="PLG72_01630"
     CDS             13311..14045
                     /locus_tag="PLG72_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79536.1"
                     /translation="MNRISKCLRFQLKNMLKSTLWFLIIFCLAAYGLSLLTYILSGGE
                     QQTFRSGLSVGAAFYIFVYVISDYRTTFNYLMINGNTRTTIYISNIIANIILSVAMSI
                     LSYLSGFADAFFTKALSGIIPGQISILQFIYPGSVGVLELPYLAALFILITAFSKLYG
                     VLNYKFGKYFVTIFWVGFGLAVISLPLSGSDSFLKIGTIIEKYLWLGHPNGVLLAQAS
                     FIITALVFGAAAYLISRRQPQTEPVS"
     gene            complement(14231..15082)
                     /locus_tag="PLG72_01635"
     CDS             complement(14231..15082)
                     /locus_tag="PLG72_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ79537.1"
                     /translation="MDGSVPFHINAEKGDFEGNGGHGRYVLLPGSEGRAEKIASMWKD
                     VAVKKHPRNHNLYLGRMESGAGDIDMAVISTGMGAPSADIIINELLNLGARNLIRIGT
                     SGSMQPERVPAGSVVVPTGSVKDESVSARYLPVEMPAVPSLEFLTAALRAWEETGAAV
                     KTPDNPESPEVFFGVVHSKDSFYAREMLVGPKRLENEEYMRILKDGGVLASEMETSIL
                     FTLGQVYSQQFVREGDRPVLTGAVLAVVGGSTPFAGKAAEASAIDAAISFCLKTVEVL
                     HRTRMQY"
     gene            complement(15153..15569)
                     /locus_tag="PLG72_01640"
     CDS             complement(15153..15569)
                     /locus_tag="PLG72_01640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79538.1"
                     /translation="MREIHPDDYYEIVELWKNTPGVGVSKADSENSILGFLLRNSGLS
                     WCYKDADRIVGTSLCGHDGRRGYIYHTAVLPEYRGRGIGAMLVRKNLEQLKAAGIDKC
                     HIFVFRDNEPGNAFWNATGWTRRNDIFVYSHDICNT"
     gene            complement(15624..15908)
                     /locus_tag="PLG72_01645"
                     /pseudo
     CDS             complement(15624..15908)
                     /locus_tag="PLG72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(16185..16844)
                     /locus_tag="PLG72_01650"
     CDS             complement(16185..16844)
                     /locus_tag="PLG72_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ79539.1"
                     /translation="MRNKEYILFDLDGTLTDPAEGITKSVAYALDHFGIRADDPGTLY
                     RFIGPPLKESFRKFYGFDDEMALKAIEKYREYYRAKGIYENRLIEGMDDLLRELSEDG
                     RQLIVATAKPEVFAEVVLEHFDIRKYFRFVCGADLHGPRISKDDVIVYALDRAGIGEP
                     SPAVMVGDREHDVIGARKAGLDCIGVLFGYGSREELQDAGASLVVETVEELGTVLLGA
                     G"
     gene            complement(16955..17449)
                     /locus_tag="PLG72_01655"
     CDS             complement(16955..17449)
                     /locus_tag="PLG72_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ79540.1"
                     /translation="MPDQERLDEIKRKLRKLKKLEIRIRFGGSFHAANYGEDIMSKGN
                     LAKGSRVRLVWGDFFNLGDKSNPLAKYRLEDLAAMDREEYKNVVDEFFFNVYYRYYTE
                     NGITGSHLYDPEILRWMGLPPDAAAEDIKRRFRELAKRYHPDTGGNSRDFIMLMENYR
                     KLVD"
     gene            complement(17579..18610)
                     /locus_tag="PLG72_01660"
     CDS             complement(17579..18610)
                     /locus_tag="PLG72_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HOJ79541.1"
                     /translation="MMDKNQVISAYLYSRMDWNAGSITGEYLTHINYSFALVRDGLIS
                     AEELDKLDEIRLVKRRFPHLKVLISVGGWGADGFSDAALTDGSRSLFAKSAAEFIRQN
                     SFDGIDIDWEYPCSSDAGIRSRPEDRENFNLLLEELRRELDRCGAEDGKRYLLTAALG
                     GTAYTLRFYDLKSISGILDYMNLMTYDFAHGSSKETGHHTCLFGPKGKTGVPSADTAV
                     KICLDNGIPADKLVLGCAFYGRGWDVADGSSNGLYASVTSECAFYSYRELVLKYIGKN
                     GFRRYWDDCAKAPYLWNGKHFITYDDPESLEHKVSYVKTNGLGGVMFWELSQDHGDEL
                     LGTLFSKLK"
     gene            18967..19851
                     /locus_tag="PLG72_01665"
     CDS             18967..19851
                     /locus_tag="PLG72_01665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ79542.1"
                     /translation="MKISEFAKMNNVTAKMLRHYDEIGLLKPAYIDEATGYRSYDASQ
                     SNCLNWIIILKELGFTLSQIREMLNGPVDAGKMIREMKQKRIEISSVLNEMIQRKIEI
                     DHLIKILEKEGFHMDKKINLLDISQDSVHEIKKNMPNMEVFLEEAYSIADASGDSKYI
                     TIFRFDINHFKQLNDDYGFDVGDRVIVACYNIIKENIEKYFERYAIGRAHGDEFIVFA
                     PVGRELAEKAAQSIIQGMSQFDFSQIGSDRQVKCCIGGVTGQNDLTIIRKMIDQSIEV
                     IYEAKGKGPNSVEIQIFG"
BASE COUNT         4841 a         5477 c         4579 g         5005 t
ORIGIN      
        1 ctggatgccc caggtattat ccaacagccc tgcataactc ttgactggct gcctgctgta
       61 acatattatt tttacataaa aaccgattcg ggggacagtt atggcatatt tcgatatcga
      121 aaaggtttct aaggaaatcg aatcttattt cggcagcgag gacgaatgcg gaaagataaa
      181 gggagccgcc gtccgccact atactgcgaa tgaaatggat aaggtacttg aaccgtacgt
      241 gggaaaacca gaggagttta tttcttttat tgaagaaaca tggggatgga aaataacgtt
      301 cgacagggac gccggtataa tcattgcaga tgaaaacaag cctttttgcg tatgcccgct
      361 ggacgacagg gtgaagtgcc cgaaactgtg caactgttcc gaaggatttg cggaaatgat
      421 gttcagtaaa gttttcggcc gtcccgtgcg ggcaactgta aaagcatccg tgctgcgcgg
      481 ggacaaatca tgtgtgtatg aagtgaaact tgcttagctg tcagcgtacc tgctcgagcg
      541 gagacgcaat gacggcttgc aaaatcgtca tctatggcta ttcgcctatt atcttgacta
      601 gtacgcactt ctcgcgtctg ccgtcgtatt cgccgtaaaa aatctgctcc caggtcccga
      661 agtcaagctt cccgttggtt atggcagcta cgacttcgcg gcccatgatc tgccgtttca
      721 gatgagcgtc cgcattatct tcgaagccgt tatgttcgta ctggctgtat ggcttctccg
      781 gcgcaagttt ctcgagccac acctcaaagt catgatggag cccgggttca tcatcgttca
      841 caaatacgct ggcagtaata ttcattgcat tgaccagcac aagcccttcc tttatcccgc
      901 tttcatcgat gcatttctgg acctgctgcg ttatgttgat ataagcccgc ctggtcggcg
      961 tattgaaata aagtactttg cggtatgact tcaaatgtac ctcctccgaa atccatccgt
     1021 attgctcaaa tcctgcctgc gccgctgaga gccgtttgcg ggtttcagcg cagtgcggcc
     1081 gcaaccggcg cttccgccgg tatccggtct ctcatctcat cgaatactca tgcagcagct
     1141 tcgcccttat gtcccacagt ttctgcgaca ggtccacgta atacctgtgg gggttctcga
     1201 acctcttcac ttcctcccac agcgtatcta cctgttcacg gcagtaatcc cttatcgtgc
     1261 gcaggtcggg agattcatag acgcatctgc ctctgtcaaa tatcttaacc ataagctttc
     1321 tggcgcggaa gttggtgacg gtctttctct tccacgtata ttccgggtcg aatatctcgt
     1381 acggctcatt gtcattgatc acttcgtcgt gcagggtgat cacatccgcc agggccttgc
     1441 cgctgtccct gtcgtaaagc ctccatacct gcttgaaagc cggattggtg atcttctcga
     1501 cattctcgct cacttttatc ttcgggatta tcttcccgtc ctcctcaacg gcgctgagct
     1561 tgtaaacgcc gccgaagaca ggctccgacc tcgaagtgat gagcctttcc ccgactccga
     1621 acgaatcgac gcaggcgccc tggctgagga tgtccctgat tatatactcg tcgagggaat
     1681 tcgaagctac gatggcgcag tccttgaaac cggcctcgtc cagcatcttc ctggccttgc
     1741 gcgacaggta cgtaatatcg cctgaatcta tcctgatgcc tttgggcctg tagcccctgg
     1801 gcacgacctc ttcgttgaat accctgatcg cgttgggaac tccggatttc aaaacgttat
     1861 acgtgtccac gagcagcgta cagttggaag gatagattcg ggcatatgcc ctgaacgctt
     1921 caagctccga cgggaacatc tgtatccagc tgtgcgccat ggtaccaagc gcaggtatgc
     1981 cgtattccct gtcggctatg gtgcatgccg tgccgacaca tcctcctatg taagccgccc
     2041 ttgcgccttc cacggcgccg ttgtacccct gggcccttct tgagccgaac tccattaccg
     2101 gccttccctg cgcagcggtg actatcctgt tggccttggt tgctatcagg ctttgatggt
     2161 ttattgacag cagcagcatc gtttcgatga actgcgcctg catgaccggc cccctgaccg
     2221 ttactatggg ctcgtagggg aatatggggg tcccttcggg aatggcccac acatcgcatt
     2281 cgaacctgaa attcctcaga tagtcaagga attcctccga gaactggttt ttgcttctca
     2341 ggtattcgat atcttcctcg ttgaacctga gctgctttat atactgtatg aactgctcaa
     2401 gtcctgccat aatggcaaaa ccgccgtcgt caggcaccct tctgaaaaac atatcgaaat
     2461 aggcgatcct gtctttcata ccgctctgca gatagccgtt cgccattgtc aactcgtaaa
     2521 aatccgtcag catcgtaagg ttgttttcaa ccaaaagagc acacctccag atgtattatt
     2581 ggccgaatat gcaggaaacc gctcatccct ggtccatgtc cagcctcatt ttgtagtcgt
     2641 ccattacaaa accgtttccg atatcggtca caatgtcttc gacgatcctg aagccaagcc
     2701 tttgatatat tttgatggac gtttcattgt acttgttcac tgtcagccag atatagccaa
     2761 gtccggattc ccgggctatc ctcattattc tgtccagcat gatcctggat atgccccgcc
     2821 ccctgcagct tttctcaaga taaaacttgc tgaggaaaag cgctttttta tccggttccg
     2881 gttttacggc aaaatatccg gcaggctttg aaccgtcata cacaatgaaa tacctgtatc
     2941 cattcgccgc gatatcattc aggatactgc cggcgctctg gaattttgac agcatataac
     3001 tgacctgtcc ggggcctatc agcggagtgt agtgctccgt ccatatttct cttgccagtt
     3061 ccgcaagttc atcagctttt tccctgtcat cctttttcag ttcaaaaagg cttatgccat
     3121 ccattgcaat actccttcat acggtatacc tgcccggaac caaagatccg tgcaggtcaa
     3181 atctactgtt attcatagca ctttatcaca aaacagtcaa taagattata aaggggacgg
     3241 atcaaatgtc cgtccccgct ccttttttta tctgtccata cccgcaggat cagcctgctt
     3301 cctcctcgcc attcaggaat ctgaactgcg aagtatacag attatagtat tgtcctttct
     3361 gcctgatcag ttcctcatgg gtgcctgatt cgactatgcc tccttcttcc acgaccatga
     3421 tcctgtcggc atttcgtatc gtcgaaagcc tgtgggctat gacaaaggag gtccttccct
     3481 cgagcagcct ccttatgccc tgctgtacga gcctttcagt gtgtgtatcg atacttgccg
     3541 tagcctcgtc aagtataagt atgcgcggat ttgccaaaag cgccctcgcg aaagctatca
     3601 attgccgctg tccagcggac agtctcgtgc cgcgctcatt cacctcggtc tggtatcctt
     3661 tctccagctt cataatgaat tcatgggccg acacggcctt cgccgcctct attacttcct
     3721 cgtcggtggc atccagcttt ccgtaccgta tgttttccat gatggtggcc gagaacagga
     3781 acgtatcctg cggcatcacg cctatctggc tcctcaggct ttccagcgtc acatcgttca
     3841 cgttgtaccc gtcgatcagc acctcgccag acgtgacttc ataaaagcgg cttatcaggt
     3901 tcacgatagt cgtcttgccc gctcccgtcg aacctaccag cgctatcgtc tgccccggcg
     3961 acacgtcgaa gctgacattc ttcaggactt cctgcccctc gtcatatccg aacgtaacgt
     4021 tcctgaatga gacgctgccc tttatctccg gcaacggctt cgtatccggc ctgtctttta
     4081 tgtcaggttc gatgtcgagt atctcgaata tcctttcagc ccccgacata ttggttacaa
     4141 gctggttata gaaattgctg atattcatta taggctgcca gaacatcgac acatagctgg
     4201 tgaacgccac gagcagtccg ggcgtgatgt tgcccgtttc gatcatgctc gcgccgaacc
     4261 agaacacaag tatcgtcccg actccccacg acatttcgac ggtgggccag aaacagtcgt
     4321 ttatcctgac ggccctgatg aatgaatccc tccactcgtg gcaaagctgc aaaaacgtct
     4381 tcgatgtttc atgttccgcc gcaaagctct gcaccaccct tatgcctgaa atatcctcat
     4441 gggtgaacgc attgaggttc gacgacttct tcctgtggac ctgccatctt ttccttgaac
     4501 gggtcgaaat gaagaagaag gcggccagca tgaacggcag cgtcgccaga gccacaagtc
     4561 caagcctgta gttcatcagc atcattataa tgaccaccga aacgatcttg acaagctcag
     4621 gtatcaggct cgtgacgctg tttgcgaaca catcgttcag cgagttgaca tctcctatga
     4681 tcctggacag gatcttgcct gtgggcctgc tgtcgaagaa ggagaacgac agcttctgta
     4741 tgtgcgaata aagctgctgc cttatcgtca gtattatgtt gttggacacc ctgcccatta
     4801 tcagcgtcct cagcctcgag catacgactg ccacgatatt gacgattatc atcgcgccgc
     4861 cgagcaggaa caggttcttc cagtcgcctt cggcaatgaa tttatcgatc ccaagcttca
     4921 tcaggtatgg attgaacagc tccaccgcaa tgacagcggc catcaggacc aatgtcttta
     4981 tgaccggtcc gatgtacggc ttaagatatg tcagcagtct tcttattata gttatattca
     5041 ggctctctat gagttcttcg tcttccctga agttgtttat cggcattata ccacctcttt
     5101 ttccagcgct tccacgtaat cgcggtactg ctcgcggtac gtttcataat acctgccccg
     5161 cagcctgagg agttcatcat gggtgcccct ctccacgatc ctgccgtctt cgaaataaag
     5221 gatctcatcg gcattcttaa cagccgaaac cctgtgggct atgataaagc aggtgaagcc
     5281 tttcctgcgc tccatcgccc tctgtatcgc gtactcggtc tccatgtcaa gcgcagatgt
     5341 cgaatcatcc agtatcagca ccttcgcatc ccggacaagt gcccttgcaa tcgatatcct
     5401 ctgtttctgg ccgcccgaca gcccgatgcc cctctctcct atcaccgtct ggtagccttc
     5461 cggcatctgg gatacgaact catcgaccat cgcgtccctg caggcagcca tgaaaacttt
     5521 gtcaggcacc tctccggctc cgaattttat attcgcctcg attgtatccg aaaacaggaa
     5581 cgtgtcctgc attacgaccg atacgttctt cctgagcgcc gcaaggtcca tttcccttac
     5641 atcgacgccg tcgaaatata cgcttcccgc agtgcagtcg taatatctcc cgatgaggtt
     5701 tatcaacgtg gtcttcccgg ctcccgtcat gcccattatt gctatcgtgc tgccgggttt
     5761 cgcatctata tttatatttt tcagcacgct gaccccttca tagtcgaagc acacatccct
     5821 gaactccaca tgtccgctta tctttttcgg taatttcggt tccgggcagt tctttatgtc
     5881 gggctcctcg gcgaacagtt tgtctatctt ctttacggaa gcgatgcact gggccaggac
     5941 gcttgtcagc catcccatca tcctgagcgg ccagatcagc atccagacgt agttgctgaa
     6001 tgccaccagc gtgcccagcg acatctcttt gccgatgacg aaataccctc ccacggtgat
     6061 gacgagcacg gacacaagat tggacaggaa ctcgatgagc gggttgtagt tcgccattga
     6121 tttcacaaga tcataattga tcctgaagtt ttcctcgttc tgcttcagga acttgagtat
     6181 ctcatgcttc tccctgccga aggccttgac gaccctgaca ccggcaatgt tctcctgggc
     6241 ggtcatattg agcaccgctc tctggtcgct gatcttctca tactgctggc cgacctgtct
     6301 ttcaagcctg aacaccacat actctatcag cggcatcgtc accaggctga ccagtgccag
     6361 cttccaactg atactgaaca ggatcaccgc agcgacgata aaatagatgg tctgttccag
     6421 gaacagcata aaaccgtaac agatggcgct cattatgtta tcggtatctt cctttatcct
     6481 tgacatcagc tctccagtgt tgctcctgtc aaagaacgca aaggaaagct tctgtatatg
     6541 gtcaaagagt tctttccgca gatccacgat cacccttgaa ctggacagat cgaacatgta
     6601 ctccttggtg tatccgagca ctgcacgtcc gaaggtgatc acggccaacg ccagcaaggc
     6661 cgtcctcagg tagggcattt cccctccaag tatgaccctg tcgatgatct ggccgacata
     6721 atacgggttg aacatatcga gcactatggc cgtcaccagt gctgcaaacc ccagaaaata
     6781 tgtgaaccaa cgctttgcta taaaccttag cagccgtttc aatcgaattc ccccttagct
     6841 ccgcgttcac aaagcttaat cgtttaccgg cagcagccgt tccatcatcg gcatgtttcc
     6901 cggatcctgc cgcagctacc catggatccc ctgcatttga tcaaaatcaa ctgccgccgc
     6961 acttattcaa ctgaactttt cctgccgtac ttctcagatc ctgccatgct tcacggaaat
     7021 ggccagccgc atcagaaatg aaaaacaccg cagctcgtgc cgcggcccat caataattaa
     7081 aggccgcgga gataacccac ggcctgcttt ttctgaaatg ctgaacttca gaaagtactc
     7141 ccgggcaagg cagggttatc tgatgccatt cctcttatat tgagttttgc tgcattttcc
     7201 agtgtgatct tttttctgtc gagtacgcct gacatcttcc tgcctccttc ctttctgttt
     7261 tatagactca cataacgata tcttaattta aaaccggcca atagtcaaga agaaatttac
     7321 attcctcagt tgccaaactt ttttataaca aaatattatt ccagcttctt cagcccatcc
     7381 ttccgctctt ttttgagtcc ttcctgttcc tcgtcgtatt ctttttccag cttcattaac
     7441 ttttcataag tactgttgtt gatcacgccc gtcacgtaca gtttgttgtc cttctggaac
     7501 tccatgacgg cttttttcag cgaactgccg aatataccgt cctcgccgcc gtgatagtaa
     7561 cccagctttt tcagcgcctt ttcaacggcc aggacatcag atccgacatc cccgtccctc
     7621 atcggataaa acggcctgtt ctcatggacc acagtgacaa gcgtattgta cgggacgagt
     7681 ttgtataact ccctcgcttc cttgttgcgc atcctgatgc atcccctgga gttgttgctc
     7741 ttgcctatgg cccacggctc agttgtcccg tgtatgccgt acttccccca gggcacgttg
     7801 aaaccaagcc acgcgcctcc gaagccttca ccccatgtat ccttgcttat gatcctccat
     7861 gttcccaggg gcgacggcga actgggtttg ccgccggaca cctcgtactt ttttaccagc
     7921 tcgccgtctt tataaacata catgagctta tcatccaggt cgatatatag gtgccagccc
     7981 tcaggactga cattgtgttt cgtctccttc ggcttttctt cctcatacac ggaactggca
     8041 tcctcacgct ggtcactgct gccggtttcc ggcctgacgc cttcgtccac ggatccgttg
     8101 ccggtatccc caccgggccc gccgctttcg gcagaagttt cgatatccgc atcggcagct
     8161 gattgctccg gtacatattc atccgcattt tcatcggaca cgtccgcctg ctgccgttcc
     8221 ggcccactgc agctgcagtt gccatgtccg tcggcatcac cggtgatcag gccggttcct
     8281 gcggacacat attccgtatc ccttgccaaa tacacgcaaa ccatttcggg catattcacg
     8341 gacacataca ggatcaccgc aatgacaagc atgaagtaca ggatcgtatt tatgtttttc
     8401 tcagacaagg catgcaccac ctgacgcata aatgcactaa taaaataata tgctgttccg
     8461 gaaacaatac aaccgtatac aagcggcagc acaacagcat tgaaatatta ccggttgttt
     8521 accgaatcgt taaaatatct ttaaattaca ggtaaaagtg caacttttgc agaattaata
     8581 tgtcgaaata agtggtaatg aaaccaatga ttctgcataa ggtaaggagc caatgatgaa
     8641 gagattactg agcaaaacaa caacaataat actgatgacc gcaatgctga tcaccctggc
     8701 atctgcgaac gcctgcgcct cggaccgtga tccggcgata ccggcgtcat ccgacggaaa
     8761 tggcagcttt tcgaatcctg ctgcctcaaa cacgctcgcc cttgcggccg gagtgactgg
     8821 cgcagtcatg aaaagcactg cggttatgta cgcaaatcca gtactggcca acaacagcgc
     8881 cgtaaacggc aagacagacc agtcttcatc ggtcagtgca aaaaaggaga tgcgcggatt
     8941 atgggtggca acggtcgtca acatcgacta tccgtccaag ccaactacag atccggaagt
     9001 ccttaaaaaa gaagcggtcg aaatactgga caatgcgaag aacaccggat tgaacgcagt
     9061 gttcctgcag gtacggccta cagccgacgc attctataaa tccaatttct tcccatggtc
     9121 caggttcctc acaggcaccc aggggaaagc gccggacggc ggcttcgacc cgctggcgtt
     9181 ctggatagag gaagcccatg cccgcggcat tgaactgcat gcatggatca atcctttccg
     9241 cataaccaaa aagaccgcat ccgaggcaaa gcccaccgtc agttcgcttc attccagcca
     9301 tcccgcccgt aagaatccgg gctgggtcgt gcaatattcc gacggcaacc tgtacttcga
     9361 ccccggcatc ccggaggtac gcaagctcgt cgtggacggc gtcatggaac ttgtaaacaa
     9421 ctacgatatc gacggtatcc attttgacga ttacttctat cccggaaggg acttcaatga
     9481 ttcgaaaacc tatgcggctt acgggaaagg ttttaagaat ataggcgact ggagaaggga
     9541 taacataaac aagcttgttt ccgatgtata caaggccatc aagtcgtcgg gcagggacgt
     9601 ccgcttcgga ataagcccgt tcggcatatg ggccaataag aagaacaact ctcttggaag
     9661 cgataccaac gggcttgaaa catattacga ccatttcgcc gattcaagga aatgggtgaa
     9721 agacggcata gtcgactata tctgcccaca gttgtactgg aatatcggct acaaagttgc
     9781 cgactacagt aagctcctca cgtggtggaa ggatgtcgcg aacggtacat ccgtagacct
     9841 gtacataggc catgccgcat acaagaccgg cgatacggac aaaacgagcc cgtggtacgg
     9901 tgttgccgaa atagaaaggc agatgctgct caacaggaat tatcccgaag taaaaggcag
     9961 cctgttctac aactatactt cactgaaaaa cagccctgca ctcagggccc tgatcagggg
    10021 agtctacgac aggatcgacg gcaaaacctc gtcagaagtc cagttgaagg tatcgtggcc
    10081 ttcaaaggac atcacgacaa gctacaccca gtactatctt accggcgcgt cggatccgtc
    10141 aaagcctctt tacatcaacg gaaagccggt cgaatcacgt tcaccgaagg gatatttcgg
    10201 catgctggtc gacctggcaa agggagccaa cacattcacg ttcacacagg aaggcgcata
    10261 cgtgacacgc accatcacca ggtccgccgg gtcttccacc gcggcaaaga tgagcaaggc
    10321 tgaaataata gcgtcgtccg tgttcccgca atcccaggaa taccggatgc ccggcgaaaa
    10381 aataacgctg tcatgccagg cgcctgccgg agcgaaagtg accgtaaaga tcggcagcaa
    10441 aacgtacaat atgacgactg catcaaaaag cggcgggctt tataaagcta cgttcaccta
    10501 tacctataca atcccgtcct acaccgggac accgcgggtc atcgatctcg gagcgccggt
    10561 atacacgatg agctacaacg ggaccaccaa atcggtcacc gctcccgcaa aagtgggtgt
    10621 tgtcatgaag ggcgccccgt tctacgctga ggtgcagaat gaggacattg atacgtttga
    10681 aacgcccaat tcggccaacg gcgccgaata tgaactccgc aaaggcatgg tcgattacgt
    10741 gaccggcatg acaggaagct atgcccggct ggcatccggc ctttgggtca ggaaaaccag
    10801 catatccaca tatacttcaa aggcaacgct gagtccgaag gtcacatcgg cagtatacac
    10861 gacaggcaaa aaatgggata cactgaagct cacgtaccag tcatcgcttg cggcaacagc
    10921 ctcattcgat ggatcgaagc tgaaagtgaa catatctgcg gcagaatggg gcgtcattcc
    10981 tgcactgccg tcggacgctc cgttttcatc agccacattc tcaaagaacg gccacgtagg
    11041 ccagtatgta ctgacattga agtccggatc gaacatcgag ggatattata tcgagaaagt
    11101 atcggacggc atcatagtcc atataaagag gcccgtcatt gcatcgcccg gagacaaacc
    11161 gcttgaagga ataacgatca tgctggatcc cggtcacggc ggaagcgaca cggggaccac
    11221 ggggcctttg ggtaccgaat accctgagaa aaggatcaac ctcgaaaacg cgctgatgct
    11281 taagtcggag cttgagaagc gcggagcgaa agtgcttatg acaaggacgg cggacgtgac
    11341 catctcgctt tccgacaggc tgacagcctc aagggacgca aaacccgata tgttcctgtc
    11401 gatacacgca aacagcatgg ctgaaaacgt tgacatttcg aaaatacagg gcttttccac
    11461 acattacaag gaagccatcg ccaagcctct gtcgcaggcg ctctgcaatg ctgccgccga
    11521 agcagggcgc gtcaacaagg gcgtccggtg ggcgaacttc tacgtcgtgc gcggcacatg
    11581 ggcgccgtcc atgctgatag aagtcggctt cgtgccaaac ccgacagagt tcgagatact
    11641 gaccgacgat ttggaacaga aataccttgc catgaaactg gctgacgcta tcgtggacta
    11701 cttctcaagc aaacgttaac gccggtgccg gaaaaatcgc cggccggtgc gccggcaaac
    11761 ctgtatctgc gggagatgct ccaacagata cgacaaccac cgttgattca accggaaaaa
    11821 cagttcatat acgcctgtct ctgatctaat aaagaggcag gcgtttgttt atccgcgaaa
    11881 aaccgacgtt ttgtcatccg tcagacactc ccccttccgc caaacgccgc atacggccgt
    11941 caatttccca aaattgtgag aaaattttta aaaaggtatt gactataact tgcatatgtt
    12001 gtatagttag ttacacaagt aatgaaccac ttaactaatc aacgggaggt gttatattga
    12061 aactggactt cagcgacgaa aaatcgatat atctgcagat cgcggaaagt atcgaggacg
    12121 acatcataaa aggcgtaata gaagaagaga cccaggtacc gtctacgaac cagatggcgg
    12181 taatatacaa aataaaccct gcgacagcag gcaaaggcat caacctgctc gttgacaaag
    12241 gagttctgta taaaaggaga ggaataggaa tgttcgttgc cgccggcgcc agaaagaaga
    12301 tactggaaga acggaaaaac agcttttaca gcagctatat cgtcacgctg ctggaagaag
    12361 caaaaaaact cgggatcggg acagacgaga tcatcgacat gatacggaaa ggagagaata
    12421 ctcatgagcg ataatatttt gcttgaaacg agatcattga caaaacgtta cgggaaagtg
    12481 accgcactga acaatttctc ggtagcgctg gagagcaata aaatctacgg actgcttggc
    12541 cgcaacggcg caggcaaaac gacgctgctg gatataatca cctcaaggag attcgcaggc
    12601 gccggcgatg tcaggctttt cgggcaggat gcgaaggaaa accagaaggt gctgcccaat
    12661 gtctgctata tgccggagaa aaactacttc ataccggcca tgaaagtgtt tgaaatactt
    12721 gaaacatcgg caatgttctt cgacagcttc gatatggaat atgccgacgg gctctgcgca
    12781 aagttcggcc ttgacaaaag aaagaaatat aaggctcttt ccagggggta cgagtccata
    12841 ttgcgcatag tggtcggcct tgcgtcaaga gcgcccctta cgatattcga tgaacctgta
    12901 ctgggccttg atgcggcagt cagagatctc ttctacaggg aactgctgga agactacaca
    12961 agccatccac gcacattcat cgtttccact catctgatcg aagaatcggc agatgttttc
    13021 gaagatgtcc ttataatcaa taacggagaa ctcatatgcc gtcaccaggt agccgaactc
    13081 agggaaatgg cattctacgt gaccggcagg ccgtcagacg tggatgccgc cgtgaacggt
    13141 atgaaagtca tccacagcga aatggtcgga agcgtaaaag tatccgccgt tttcaataaa
    13201 atcaacgatg aaatgaaaaa tcggctcaag acatgtaacg tcgatatttc accgataccg
    13261 gtgcagaaac tgtttatcta tctgaccaac agtaacaagg gggtttttca atgaacagga
    13321 tttcaaaatg ccttaggttt cagttaaaga acatgctgaa aagcacactg tggtttttaa
    13381 tcatattttg cctggcagct tatggcttat cgctgctgac gtacatcctc agcggcggag
    13441 agcagcagac attcagatcg ggtttatctg tcggggctgc attctacatc ttcgtatatg
    13501 ttatatccga ttacagaact actttcaatt accttatgat aaacggcaac accaggacca
    13561 cgatctatat ttcgaacatt atcgcaaaca tcatactttc tgttgccatg tcaatattgt
    13621 catatttatc cggattcgca gatgcctttt tcacaaaggc cctttccggc attattcctg
    13681 ggcagatctc catccttcag tttatctatc cgggttccgt cggtgtgtta gaattgcctt
    13741 atttggctgc actattcata ttgatcacag cattttcgaa gctgtacgga gtacttaact
    13801 ataagttcgg caagtatttc gtcaccatct tttgggtcgg ctttggcctt gcggtcatat
    13861 cattgccgct gtcgggcagc gacagcttcc taaagatagg aacgatcatt gaaaagtacc
    13921 tgtggttggg ccatccgaat ggcgtgcttc tggcccaggc cagcttcata ataaccgctt
    13981 tggtgttcgg tgctgccgca tacctgatct cacggcgcca accgcagaca gaaccggttt
    14041 catgaccgca tcgggcacca ggcggcagtc taaatgaata aagccggaag gaataacaca
    14101 tgatcagaat ggctgcagca tccggacaac tgcactggaa atcagcaata tgcaaggcga
    14161 tacatgcctg acatattgct gatttttcac tcttttcctg cttcatcaat ctttccgcac
    14221 atcaccgttt tcagtattgc atgcgtgtcc tgtgcagtac ctccacagtc ttcaggcaga
    14281 aggagatcgc cgcatcgatg gcagacgctt ccgcagcttt gccggcaaat ggcgtagatc
    14341 cgccgaccac cgccaggact gcgcccgtga gcacaggcct gtccccttct cttacaaatt
    14401 gctggctgta gacctgccca agcgtgaaca gaatgcttgt ctccatctcg gaagccaata
    14461 ctcctccgtc tttcaggatg cgcatgtatt cctcattctc caggcgtttc ggccccacga
    14521 gcatttccct ggcgtaaaag gaatcctttg aatgtacgac tccgaaaaat acctccggtg
    14581 attcgggatt gtccggtgtt ttcaccgctg ccccggtctc ctcccatgct ctgagcgcgg
    14641 ctgtcagaaa ctcgagcgaa ggcacggcag gcatttccac agggaggtac ctggctgaga
    14701 ccgattcgtc cttcacactg cctgtcggca cgacaacaga tccagccggc acacgctccg
    14761 gctgcatgga acccgatgtt cctatcctta ttagattccg cgccccgaga tttaacagtt
    14821 cgtttatgat tatatcagca cttggcgcac ccatgcctgt ggatatcacg gccatatcga
    14881 tgtcccctgc cccggactcc atcctgccaa ggtacaggtt atggttcctc ggatgtttct
    14941 taacggccac atctttccac atcgacgcga tcttttccgc ccggccttct gagccaggca
    15001 gcagtacata gcgcccgtgg ccgccgtttc cttcgaaatc gcctttttcc gcattgatat
    15061 ggaacggtac ggatccatcc atgcccaaca ccccttcctt tgccggtata tcagtccatt
    15121 ccgggcattg ccggctactg ccgcagaatg cgttaagtat tacagatgtc gtgtgaatac
    15181 acaaaaatat cgttgcgcct ggtccagccc gtcgcgttcc agaaagcatt gcccggctca
    15241 ttgtcccgga acacgaaaat atggcattta tctatacctg ccgccttaag ctgttcgagg
    15301 ttcttccgga ccagcatggc gcctatgcct ctgcccctgt attcgggcaa taccgcagtg
    15361 tggtagatgt aacctctcct gccgtcatgg ccgcacaggg acgtgccgac tatcctgtcc
    15421 gcatccttgt agcaccagct taggccggaa tttctcagta aaaagccaag tatgctgttt
    15481 tcggaatcgg ctttgctgac accgactccg ggagtattct tccatagttc gacgatttcg
    15541 taatagtcgt ccggatggat ttccctcatg cctgtatcta tatccaaccg gatccagtac
    15601 aggcacctgc cgtccacgat tttatccagg acgccgccgt tgttttcgat aacacgcctt
    15661 gaaccggtat ttccctcata acatattata agggcacggg ggatatttat ctccgccgcc
    15721 ttcttgagca tttcccgaag aattatcgtc ccgtagcctt tccttctttc agaaggccgg
    15781 atgcaataac cgatatgccc tccctctttc ctgagcttac cggttaggta cttcctgagc
    15841 ttggccatcc cgacgggcct tccgtcagca aacagccagt aaaccgcctg cgggacaaat
    15901 cccggcataa gccctatccc tcgcgacatg tctatccatc ggtatataag gtccttgaaa
    15961 tcctctggtt tcgtattgta tgcactgttc tggaacccgt tttcaccggg gccgatctca
    16021 tgcagcattt caagcacgtc atacccgtcc tgcggagaac actcgcgcag ttcgatctcc
    16081 ggcattcgtc cgttcgatcc ggtatacagt ctgctgtcaa aagtcccgtt tctcatttca
    16141 gcctccacat ttaatttttc gtatcatccg gcatattttg aatatcaccc ggcgccaagc
    16201 aatactgtcc cgagttcctc cactgtctca accaccagtg aagcgccggc atcctgcagt
    16261 tcctcacggc tgccgtagcc gaacaggacg cctatgcagt caagtcctgc tttgcgcgcg
    16321 cctatgacgt catgctcccg gtcgcctacc atgaccgccg gcgaaggttc gccgatgccg
    16381 gcccggtcaa gcgcgtacac gatcacatcg tccttgctga tcctgggtcc gtgaagatcc
    16441 gcgccgcata cgaaccgaaa gtacttcctg atatcaaaat gttcgagtac gacttcagcg
    16501 aacacttccg gttttgcggt cgccacgatc agctgccttc catcctccga cagttccctt
    16561 aacaggtcat ccataccttc gatcagccgg ttttcataga tcccttttgc cctgtaatac
    16621 tccctgtatt tctcgatggc cttcagcgcc atttcatcgt cgaaaccgta aaacttcctg
    16681 aaagattcct tgagcggcgg gccgataaac ctgtacagtg taccgggatc atcggcgcgt
    16741 atcccgaaat gatccagggc ataagccact gacttcgtga ttccttcggc cggatccgtc
    16801 agtgtcccgt ccaggtcgaa caaaatgtac tctttattcc tcatctcatt tccctttgct
    16861 gccagtcagc gtttttctca tgtttatggt tatcaatcat aatacatact ttccatgctg
    16921 tcatgataaa tacatctggt accgcggcat ccggtcagtc caccagtttg cggtaattct
    16981 ccatcagcat gatgaaatcg cggctgttcc cgcccgtatc aggatggtat ctctttgcaa
    17041 gctccctgaa ccgccgcttg atatcctcag cggcggcatc cggcggaagc cccatccatc
    17101 tgaggatctc cggatcgtac aggtgggacc ccgtgatccc gttctcggtg tagtacctgt
    17161 aatatacatt gaagaaaaac tcatcgacga cattcttata ctcttccctg tccatcgccg
    17221 ccaggtcctc aagcctgtat tttgccaatg gattgctttt gtcgcccagg ttgaaaaaat
    17281 caccccagac cagcctgaca cggcttcctt tagccaggtt tcccttcgac atgatgtctt
    17341 cgccgtaatt ggccgcatgg aaggatccgc cgaaacgtat cctgatctca agtttcttca
    17401 gtttcctcag ttttcttttg atttcatcaa gccgctcctg gtccggcatc ggtcgatcct
    17461 cccctataca gcattttctg cgtgttcccg ggacaatgcc gctgctcttg ccaaatacat
    17521 gcagtggatg atcttacgat gtgtgcagcc attgtatgcc aagttcacct tatcatattt
    17581 atttcagttt tgaaaatagc gttccaagca gttcatctcc atggtcctgc gaaagttccc
    17641 agaacatgac gcctccaagt ccgtttgttt tgacgtagga aaccttgtgc tccagcgatt
    17701 cgggatcgtc gtacgtgata aaatgcttcc cgttccagag gtacggggct ttcgcgcagt
    17761 catcccagta ccttctgaag ccgtttttac cgatatactt caaaacaagt tccctgtaac
    17821 tgtaaaaagc gcattccgag gtcacggacg catacagccc gttcgacgag ccgtccgcga
    17881 catcccatcc cctgccgtag aaggcgcatc ccaaaaccag tttgtccgca ggtatcccgt
    17941 tgtccaggca tatcttcact gctgtatcgg cagaaggtac gccggttttc cccttcggtc
    18001 cgaaaaggca ggtatggtgg ccggtctctt ttgaagaacc gtgtgcgaaa tcatatgtca
    18061 tcaggttcat gtagtccagt atcccggata tgctcttcag atcatagaag cgcagtgtat
    18121 atgccgtccc tccaagagcg gccgtcagca ggtacctttt cccatcttcg gctccgcacc
    18181 tgtcaagttc ccgtctcagt tcctccagca gaaggttgaa attctccctg tcctcgggcc
    18241 gcgatctgat ccctgcgtcg gaagaacacg ggtattccca gtcgatgtct ataccgtcaa
    18301 aagagttttg tcttataaat tcggctgctg atttagcaaa cagcgatctc gatccgtcgg
    18361 tcagcgccgc gtcggaaaac ccgtcggcac cccagccgcc taccgagatg agcaccttca
    18421 ggtgaggaaa cctccttttc acaagcctga tctcgtccag tttgtccagt tcctctgctg
    18481 atatgaggcc gtccctgacc agggcgaaac tgtagttgat gtgcgtgagg tattcgcctg
    18541 ttatgctgcc ggcgttccag tccatcctgc tgtaaaggta ggctgatatg acctggtttt
    18601 tatccatcat ttttccccct ttccctgata cggcattaat acatttatat catatgccga
    18661 ctgtcggaac aagaaaagtt tccttttcct gccgtttata gattatattt atgttcacta
    18721 ttgaccttcc cactacggga aggtttatag tagtagtaga aaataccggc cgcgtatttt
    18781 tataaaggag ggagtgcctg acctcgtttt ttattccgga ataagcaaag atgtctttta
    18841 tgaacgttca aaattccatg gattattgca taatctgccc ggacggttca ttttgaagat
    18901 gatgaaaatc taaggaagag tgagatattt aaagaaaaat gaagatctaa caggaggtct
    18961 gttcatttga aaataagcga attcgcaaaa atgaacaatg tgactgcgaa gatgctgcgt
    19021 cattatgacg agatcggcct gctcaagccg gcatacattg acgaggcgac aggctaccgt
    19081 tcatatgacg caagccagtc caactgtctc aactggatca taatactcaa ggagctcggt
    19141 tttactctgt cccaaatcag ggagatgctg aacgggcccg tcgatgcggg gaagatgata
    19201 cgggaaatga aacagaagcg catcgagata tcttcagtac tcaatgagat gatacagagg
    19261 aaaatcgaga tcgaccatct gatcaaaatt cttgagaagg agggctttca tatggataaa
    19321 aaaataaacc tgctggatat ttcccaggac agcgtgcatg agatcaaaaa gaacatgccg
    19381 aacatggagg tattcctcga agaggcatac agtatcgcag atgcaagtgg tgacagcaaa
    19441 tacataacga tattccgttt cgatatcaac catttcaagc agctaaacga cgactacggc
    19501 tttgacgtcg gtgacagggt catagtcgca tgttacaaca tcataaagga aaacatcgaa
    19561 aagtattttg aaaggtatgc gataggccgt gcccacggcg acgaattcat agtattcgcc
    19621 ccggtcggaa gggaactggc tgaaaaagcg gcccaatcca taatccaggg tatgtcgcag
    19681 ttcgactttt cacagatcgg cagcgacagg caggtaaaat gctgcatagg cggtgtaaca
    19741 gggcagaacg atcttacgat aatacgcaag atgatcgacc agtctattga agtaatatat
    19801 gaagcaaaag gaaaagggcc gaattcagtt gaaatccaga tattcggata gagatacgca
    19861 gacaggggcc gcagttattt gattatcctg tccgaaaccc tt
//
LOCUS       DAOSCC010000014        19830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_1929_length_19830_cov_4.497750, whole genome shotgun sequence.
ACCESSION   DAOSCC010000014 DAOSCC010000000
VERSION     DAOSCC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19830
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1929_length_19830_cov_4.497750"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /locus_tag="PLG72_01670"
     CDS             <1..1644
                     /locus_tag="PLG72_01670"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ79543.1"
                     /translation="VDLEGDDFDIISLHGSWARERHIQRIPAGLGKKVFSSNRGEPGH
                     QCNPFIAVVGSDATEDFGEVYGFNFVYSGNFCACVEGGQFETTRVVMGINPTDFEWLL
                     EPGEQFVAPEVVMVYSYQGIGAMTRTFHDLYRGHLIRSPYRNEKRPVLINNWEATYFN
                     FDTEKLLAIAKEASALGIEMLVMDDGWFGRRNDDNCSLGDWVVNEEKLKGGLKALVDE
                     VNKLGMKFGIWFEPEMISPDSDLYRAHPDWCIHIEGRAPGLARNQLVLDLTNPEVVET
                     VYGMMKKVLTEANIAYVKWDMNRPLSDLGSNWLPRERQGELCHRYVLAVYRMLDRLTT
                     DFPYILVEGCSGGGARFDPGILYYCPQIWCSDDTDAIERLAIQRGTAMVYPLSTIGAH
                     VSDCPNHVLGRTTPFETRGYVALAGTFGYELDVTRIPKEDREMIPEQIKMYHKYNDLV
                     REGDYYRIADYCENHLYDAWMVVSKDKKEALVTYIQVMQRPNYKSRRIRLKGLDEDTV
                     YRDEKTGETYSGSALMYAGINITGLHGDFKGKLIYFTAV"
     gene            1736..2200
                     /locus_tag="PLG72_01675"
     CDS             1736..2200
                     /locus_tag="PLG72_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOJ79544.1"
                     /translation="MKTGIIVYSHTGHTLSVAQKLEQAIKAAGHAVSLERVEPVDDNP
                     NSNAPVQLKSMPDVLQYDAVIFASPVHAFSLARAMKTYLSQLPDMTGKKVSCFVTQQL
                     KWPWMGGYHVVRQITEVCRAKGAEIISSGVVNWSRKNRERQIDDIVRRLSIL"
     gene            2258..3094
                     /locus_tag="PLG72_01680"
     CDS             2258..3094
                     /locus_tag="PLG72_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HOJ79545.1"
                     /translation="MKTKPQSWTNCDKGIRDYITGLVDLFRHRLGSNLAGVYLHGSLA
                     MGSYFPPKSDIDLIIVVYNGLDAGFAGSLNRSIAGYAEKRPTTGSIELSVITLGTART
                     VPSQMPYELHYSEAWHRMVLDDRVEYGKHFTDPDLPAHLMCVKKRGVCLYGKPVDEVF
                     GEVDWNDFMKSVLADFDWTVSDKNICESPYYCILNICRVLQLLEEDGRQILSKYEGGL
                     WGIRNLPQEYSALVGKALEVYSSDRAVDEAERKTGGVTWDRAELLAFRDYAKSRRERN
                     RK"
     gene            3131..3934
                     /locus_tag="PLG72_01685"
     CDS             3131..3934
                     /locus_tag="PLG72_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ79546.1"
                     /translation="MRTADINYDINLEMRGVGHVTREVIQIGHHGRTIYGIAEFPDGT
                     GKWPIVIFCHGFNGSADDFAEYSEYLVSRGVAAYRFDFCGGSVNSRSSLKTYEMSLFT
                     EKEDLIAVLENVRKWDRVDANNIFVFGMSQGGAVAALTADEHGADIKGLVLDFPALCI
                     PDDWNKRFPTRESIPDLYEFWGVTLGRAYFEAVHGYDIFEHIGKYDRQVLIFHGDKDN
                     VVPVGYGQRAASLYPNAKIEVYQGEGHGFSAPYHWRVIEKTYDLVMQNK"
     gene            4021..5355
                     /locus_tag="PLG72_01690"
     CDS             4021..5355
                     /locus_tag="PLG72_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ79547.1"
                     /translation="MANPYLPNWEYIPDGEPRVFGNRVYVYGSHDNAASGKFCDYKLK
                     VWSADVNDLNNWICHGDSFHTRADRDHESDTPWTDGELYAPDVVEKDGKYYLYAYIFY
                     TKGCVAVSDRPEGPFRLISQYSVPAVYENDMDKGVFCDPGVLVDDDGRVYVYWGFERS
                     YMAEIDPDNMHEILPGSVRKDIIPAGNRPSDFYEACSPRKIGDTYYLIYSPRKGSRLD
                     YAVSRSPMGPFEYGGTIIDNGIDYPGGNNHGSICKINGQWYIFYHRMTNNSVFSRRAC
                     VERIEILSDGRIPQVEMTSLGFEKSLSPYRYTPADIACVLKGGCYITERNIFQRPVVN
                     ITNGCVVGFKYFDFGEDYSSRTMEFSAKIRGCGSEGQVRILIDDWKDGMEIGRCDIGP
                     DDGILYSVVRCVTGRHALYFVFEDKYGDVWEWAKGGFENRPICELEGFVFSK"
     gene            5445..6314
                     /locus_tag="PLG72_01695"
     CDS             5445..6314
                     /locus_tag="PLG72_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ79548.1"
                     /translation="MHFKEAKSILSSQNGMNIYRGCSHGCIYCDSRSKCYNMEHEFED
                     IEVKVNAPELLEAALKKKRKKCMISTGSMCDPYMHIEEKLGYMRKCLEIIDKYGFGVT
                     VLTKSNRILRDLDLLTSINRRSKCVVQMTLTTYDENLCRILEPAVCTTRERFEVLKIM
                     NQNGIPTVVWLDPILPFINDTEENLRGILDYCAKSKVYGIICFGMGMTLREGNREYFY
                     SKLDVHFPGLKQKYHKKYGYSYEVTSDNNDKLMDIFYRECRKHGIVCDNAKIFRYLHT
                     YEVRQPEQLSFFD"
     gene            6474..7286
                     /locus_tag="PLG72_01700"
     CDS             6474..7286
                     /locus_tag="PLG72_01700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ79549.1"
                     /translation="MYLELCALLKKPDLYERTHEKFWNDPHISKGMLEAHLDPNTDAA
                     SRRPEFIDRSVEWIISLLPEGASLLDIGCGPGLYTKRFADRGFSVTGMDLSEHSISYA
                     REHDKKSNYVFMDYLQMDYENAFDMITLIYCDYGALIPVERAGLLQRVYRALKPGGRF
                     LFDVFTPVYTSGIQEGTSWEICRQGGFWSDEPYICLNAQYRYNENISLDRYVIIKENA
                     VHCYNIWDTSFTTDFLLSEVLPAGFIYDGFYADVAGNPYYEGSETLCAVFRK"
     gene            7379..8179
                     /locus_tag="PLG72_01705"
     CDS             7379..8179
                     /locus_tag="PLG72_01705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOJ79550.1"
                     /translation="MAKPDITLRLERPEDYHAVEELTREAFWRSIRGFCDEHLLVHRL
                     RKVPVFIPELDYVAEADGRIVGNIIYTRARIEDPSGITHEVLTFGPLSVLPEYQNMGV
                     GKALMLHTFEKARKLGYRAIVIFGHPDYYPRVGFRRASEFGLTTSDGNTFDAFMALPL
                     YEGALDGIQGRFFIDPVFESLDDKDVLEFDKSFPPKDRYVPVPIEVLLDRLDAGAREA
                     VEGLGCTYLDEFTHRSERDISSATGLDEKAMDIVRQVMLEHGWRWGSK"
     assembly_gap    8346..8445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8768..10501
                     /locus_tag="PLG72_01710"
     CDS             8768..10501
                     /locus_tag="PLG72_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ79551.1"
                     /translation="MKNGIRKLISVVLCFTLLFSVSSLAFAKSAQSNSKQFKDVKPGH
                     WAYDAIMWMLERSIIDGVGGDRFDPNGIVTRAQFAKMMVNTLNLKKYSPETPSFLDVK
                     KNSWEYPYVESAKPYLTGFRTSSGDYFKPSLAAVREDMAVALVNALGYQGESIDTSIL
                     NQFADKDQISPNLRKHVALSVKYGLIVGKTENGRLLFDPQGNLTRAQAAVLLKRAFEK
                     NEEKVTYDEEKVTYDPNTGYTYERPIIELKTENNVQVIRWNRIESDKFREYRVVISKN
                     DDTPQYPDNGYLYTITDRNRTYAVIDNTDKYNGGDFGNYLTKGQTYYISVTAVYSDRN
                     VPGNTVSFVYNGPENPELYVAPDVSSSVENGKLVLRWNRIDSPKLTGYIVSASKNDST
                     VTYPENGYLYKINDVNRTYAVIDNSTRYTDGDFEGYFINGEQYYFSVTAVYNDKSVTG
                     NTIRCTYVGEDSPELFPAPQVSAAYEDGKLVVKWNKLDSSLLTEYRVVISEKSDKPAY
                     PANGYYDQAYGKDTTSVVIDSAKQYHNGDFEKLTYATEYYISVTAVYGNKYVAGNAVK
                     ILYLINDQQAE"
     gene            10684..12084
                     /locus_tag="PLG72_01715"
     CDS             10684..12084
                     /locus_tag="PLG72_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ79552.1"
                     /translation="MGGIFGVVSKNDCVMDLYFGTDYHSHLGTSRGGMAVFSGNGFSG
                     YIHNIENIQFRAKFESDLAEMSGNMGIGCISDTEAQPLTVCSHHGHYALTTVGRVNNI
                     DELVKKAFQRNYIHFLEMSRGEINPTELVAALIDQEETIKDGILGAQEAIDGSCTMLV
                     LTHEGIYAARDRLGRTPLLVGKKDESYCVSLESSALPNLGYSLEYELGPGEIILLTPE
                     GIVPLAPPRQEMKICAFLWVYYGYPASTYEGRNVEIMRYKCGQALARGDTREVDAVAG
                     VPDSGIAHALGYSNESGIPYSRPLVKYTPTWSRSFIPQEQSVRSLVAKMKLIPIPELI
                     RNKRLLICDDSIVRGTQLGQTVALLHYFDAREVHVRPACPPLLFGCKYLNFSRSRSEM
                     DLITRRSIVEIEGREPSGFDEYCDPKTEKYHCMVECIRKKLSFTSLRFQKLEDMIDAI
                     GLPACKVCTYCWNGRE"
     gene            complement(12119..12997)
                     /locus_tag="PLG72_01720"
     CDS             complement(12119..12997)
                     /locus_tag="PLG72_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOJ79553.1"
                     /translation="MAGYKRRFGDRYDGRRLRSLDPFFKIIPYIMRSRLDSQIFFEEK
                     IDITHAEAYLRKRNKTSDTRLSMLHIFIAAAVRTLALRPALNRFVAGQKLFARFEYLV
                     SLAIKKEMTFDSPETTIIFKFEPTDTLYDVARKVEQQVKENKEAESDNTTDKTARIVT
                     LCPGFIVKFIVFVVRLLDYFGLMPKIIYKASPFHTSVFITDVGSLGISPVYHHLYDFG
                     TTSVFVSFGTKRTEKVIDSDNNIVNRRFITMRIVCDERIVDGHYFASAVKLYKTLIES
                     PEKLEVPPVKVVPDVD"
     gene            13376..14071
                     /gene="deoC"
                     /locus_tag="PLG72_01725"
     CDS             13376..14071
                     /gene="deoC"
                     /locus_tag="PLG72_01725"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ79554.1"
                     /translation="MVLTVNDIAQMFDLSCVRTNSDEKDIKDLAENARRYNCGHVSVM
                     QCFIPVIKKLLSDRPDIKVVGNVSFPSGSDTTSVKVFQAVELRYAGCDEIDMVINVGK
                     LRSGRYAEVEDDVKAVVEAADRLPVKAIIEVSCLDRKEVEKACEISIKAGAAFIKTGT
                     GWMPGGTTIEDIKLIKSIVGDRIRIKASGGIRTLDTLLEMYSLGVTRFGVNMCSGIAI
                     LDECIARGGEVTI"
     gene            14221..15759
                     /locus_tag="PLG72_01730"
     CDS             14221..15759
                     /locus_tag="PLG72_01730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ79555.1"
                     /translation="MGNRIISYDLGTGGNKASLYDFEGNCLASVFIPYDTLYPRAGWH
                     EQRPEDWWHAVVLGTRKLLEKTGEDRKNIECLAISGHSLGAVPVDKNGRLLRKTASIW
                     SDARAQEQARKFFANVDEHEWYMTTGNGFPAALYAVFKIMWYMDNEPDIYRDTFKIIG
                     TKDYINMKLTGVVCTDNSYASGSGVYDLKWNRYHDGYIGASGISADKLPEIVPSSEIL
                     GTVTPEASEETGLGTNARVVCGGVDNSCMALGAKNLEEGRVYTSLGSSAWIAVSSEDP
                     ILDLKARPYVFAHVIPGMYTSAVSIFSAGTSFRWLRDNICADLKMQELDGGKNTYAIM
                     DELAEKSPVGANRLLFNPSLAGGTSQDASANLRGAFMGLDLKHDRADLIRACMEGIAM
                     NLRLRLDVLKKHCRLQNEMLLVGGGSKSPLYRRIFADVYNMDIVKTNIDQDAAALGAA
                     GVAAVGCGIWKDFSRIDEIHRTTEIMKPDPANNAKYEKILEVFSFASDRQSEISDRLF
                     ALEL"
     gene            15889..16629
                     /locus_tag="PLG72_01735"
     CDS             15889..16629
                     /locus_tag="PLG72_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ79556.1"
                     /translation="MLDKSRPIPLYYQVKESLLEKIKSRQFNVGDLIPSEAELQEQYN
                     VSRITVRRAIQELVQEGHLYTKQGRGTFVSRPKVSQELNLITSWAETMEEMGMRPETK
                     KIEYSEEPAPVNIARLLNVPIGDMVYKIERLRYADGEPTCIMTNYLSPDVVPGLLDKG
                     IKGESLYETLEKHYNIVLSHAEETVEAKAAKTSEAELLNIKRGAPLLHATRVTYDITD
                     RPVEVVISISRADRYSYKIRLSGRKKTI"
     gene            complement(16973..18034)
                     /locus_tag="PLG72_01740"
     CDS             complement(16973..18034)
                     /locus_tag="PLG72_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ79557.1"
                     /translation="MARMMRAQVIEAPGKMVLKQIPVPEISPDEVLIKVSMCGICGTD
                     WKIYKGEYASEFLPMISGHEFWGVIEEVGSNARGLKKGDRVAVDICLPCGTCYYCRRG
                     DELLCETFTQLGIHTDGAFAEYVKAPWTNCYLIPDEVDALSAAFVEPLTAVMQAAKKM
                     DVAIASSVVVIGTGLGILHGALAKLRGAAPVIVIGRSESKLRLAKQLGADFTININEV
                     DDPVAEVKRLTGGVGADYVLESVGTPETYEQAFSMVRRGGKVEAFGICPPDRKACFSP
                     YEFVLGEKKVSGSCAGIGNDWGDVLTLLKYKRIDPTPMFSMIVPLEELEQALHELRTN
                     KDLIKVFVDPSATERKILR"
     gene            complement(18068..19054)
                     /locus_tag="PLG72_01745"
     CDS             complement(18068..19054)
                     /locus_tag="PLG72_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ79558.1"
                     /translation="MKLGFFTANFTERPLEEVAKLAAEHGYEALEIPAYEGNGQFEPR
                     EVLEGDNAKKIKKMVEGYGLTISALSNHADSLLILGPHGVDTDTIYKGTKEEKIRFGT
                     ESLLRTAQAANALEVPVVNGFTGVTNFGRYFPFPYAQGWADIEKEFVELFVPILDKFK
                     EYGVKFAVEPHPNNFIYDIHTAEKALKLVDYHPCLGFNLDPANLIYLGLRVENFIDRL
                     GDRIFHVHAKDGEIVEHNLPVGGILMQGEWQRLDRAFRFRIPGWGSVPWKKVITELSM
                     VGYNYVLSYEHEDVTMSRMDGVVKTVAFLKPLLIGAPYEGRQDKLFKGKDVL"
     gene            19385..>19830
                     /locus_tag="PLG72_01750"
     CDS             19385..>19830
                     /locus_tag="PLG72_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HOJ79559.1"
                     /translation="MDYKKTLLSPVKIGTRISPNRFFIQAMECTDADKDGNPSDLTYK
                     RYENLFRGEAGVVTLEAISITDKSRSRQEQLLIMPRNEKALANFVKRMKEINPNSLFL
                     FQLTHSGELSHPDFSRRVTVEPLPGFGGELLSEEEVDRIMDQFVLAV"
BASE COUNT         5263 a         4439 c         5285 g         4743 t
ORIGIN      
        1 gtcgacctgg aaggggatga cttcgacatc atatcgctgc atggctcgtg ggcgagggaa
       61 aggcatatac agaggatccc tgcaggactg gggaaaaagg tgttctcttc gaaccgcgga
      121 gagccaggcc atcagtgcaa ccctttcata gctgtcgtcg gatccgatgc gacggaagat
      181 ttcggtgaag tctacggttt taactttgtt tattccggta acttctgtgc ctgcgtcgaa
      241 ggcggacagt ttgaaacgac cagggtggtc atgggcatca acccgactga tttcgaatgg
      301 ctgctggagc ccggtgagca atttgttgcc cctgaagtcg taatggttta ttcatatcag
      361 ggcatcggcg ctatgacacg gactttccat gacctgtacc gcggccatct tataaggagc
      421 ccgtacagga acgaaaagcg tcctgtcctt atcaacaact gggaggcaac ttattttaac
      481 ttcgataccg aaaagctgct ggcgatagca aaggaagcgt ccgctctcgg catcgagatg
      541 ctggtaatgg acgacggctg gttcggcaga cgcaacgatg acaattgctc actcggcgac
      601 tgggtcgtaa atgaagagaa attaaaaggc gggctgaagg ctctcgttga tgaagtgaac
      661 aagcttggca tgaaattcgg tatctggttt gagcctgaaa tgatctctcc tgattcggac
      721 ctgtaccgtg cccatcctga ctggtgcata cacatagaag gccgcgctcc cggcctggcg
      781 cgaaaccagc tggttttgga tctgacaaat cccgaagttg tcgaaactgt ttacggcatg
      841 atgaaaaaag tactgacgga agccaacatt gcttatgtga agtgggatat gaaccgccct
      901 ctgagcgatc ttggcagcaa ctggctgccg cgcgaaaggc agggcgagct ttgccaccgg
      961 tacgtgcttg ctgtctatcg gatgctggat cgcctaacga ccgatttccc gtacatcctg
     1021 gtcgaaggct gctcaggcgg cggtgcaagg tttgatccgg gcatactgta ttactgcccg
     1081 cagatctggt gctccgacga taccgatgca atagaacgcc tggcaataca gcgcggcact
     1141 gccatggtgt atccgctttc cacgatcggg gcgcatgtga gcgactgccc caaccatgtc
     1201 ctcggcagga caacaccctt tgagacaaga ggatacgtgg cgctggcggg aacgttcggg
     1261 tatgaacttg atgtgacgag gataccgaag gaagacaggg aaatgatccc tgaacagata
     1321 aagatgtatc acaagtacaa cgacctggtc cgcgaaggcg actactacag gatcgccgat
     1381 tactgcgaga atcacctgta tgatgcgtgg atggtcgtgt ccaaggataa aaaggaagcg
     1441 cttgttacat atatacaggt gatgcagagg ccgaactaca agagccgcag gatacgcctg
     1501 aaagggcttg acgaggatac cgtctacagg gatgaaaaga ccggagaaac atactcaggc
     1561 agcgcactga tgtatgccgg tataaatatc acaggcctgc acggcgattt caaagggaag
     1621 ctgatctact ttacggcagt ataaagcgga agtttccggt ttgggaccct ggacgatatt
     1681 tttcgtatat tggtatgtta aaggctgtaa aagtttatag ataggggatg tgaatatgaa
     1741 aacaggtata atcgtttatt cccataccgg gcatacgctg tcggttgccc agaaacttga
     1801 gcaggccata aaagctgcgg gacatgcagt cagcctggaa agagtggagc ccgtggacga
     1861 caacccgaat tcgaacgcgc ctgttcagct taaatcaatg ccggatgtat tgcagtatga
     1921 cgcggtgatt ttcgcatcgc cggtacatgc attctccctg gcgcgtgcta tgaagacgta
     1981 tctttcgcag ctgcccgata tgaccggcaa aaaagtcagc tgttttgtga cacagcagct
     2041 gaagtggccg tggatgggcg gataccatgt ggtccgccag ataactgagg tatgcagggc
     2101 gaaaggtgct gagatcatat cgagcggtgt cgtcaattgg tcgcggaaaa accgcgaaag
     2161 acagatagac gatattgtac gcaggctaag tatattgtga ggtgcgacag atcatacggt
     2221 ttgccgtacg taccctttaa cagtcatgga gcctcatatg aaaaccaaac cgcagtcgtg
     2281 gacgaactgc gataaaggga tacgcgatta tattacgggt ctggtggatt tgttcaggca
     2341 caggctgggc agcaaccttg ccggtgtcta tctccatggt tcattggcga tgggcagcta
     2401 cttcccgccc aagagcgata tcgacctcat aatcgtcgtt tataatggtc ttgatgccgg
     2461 ttttgccgga agcctcaacc ggtcgattgc cggatatgcg gaaaaaagac cgacaaccgg
     2521 aagtatcgag cttagcgtga taacgctcgg gactgcacgg accgtgcctt cccaaatgcc
     2581 gtatgaactg cattacagcg aggcatggca ccgcatggtg ctcgacgacc gtgtcgaata
     2641 cggcaaacat tttaccgacc ccgaccttcc cgcacacctg atgtgtgtga aaaaacgtgg
     2701 agtgtgcctg tatggaaagc ctgtcgatga agttttcggc gaggttgact ggaatgattt
     2761 catgaagtcg gtgcttgcgg attttgactg gaccgtttcg gataagaaca tatgtgaatc
     2821 gccgtattac tgcatcctga atatctgccg tgtcttacag ctgcttgagg aggacggaag
     2881 acagatactc agcaaatatg aaggcggttt gtggggtatc aggaatctgc ctcaggaata
     2941 ttcggcgctc gtcgggaagg cattggaggt ctatagctcg gacagggcgg tcgatgaggc
     3001 tgagaggaag accggcggcg tgacctggga cagggcagaa ctgctggctt tccgcgatta
     3061 tgcgaaaagc cggagagaga gaaatcgaaa gtgacctggc ttacattgat gaacttgccg
     3121 aaactaactg atgagaactg ccgatatcaa ttatgatatc aatctggaaa tgagaggagt
     3181 gggacatgtg acgagggaag tcatacaaat cggacatcat ggccggacaa tatatggcat
     3241 agctgaattc cctgatggga cgggcaagtg gccgatcgtt atattctgcc acggattcaa
     3301 cggttctgcg gacgatttcg cagaatacag cgagtacctg gtatccagag gcgtggctgc
     3361 ttatcgtttc gatttctgcg gaggatccgt aaactccagg agtagtctca agacatatga
     3421 aatgtctctg tttactgaaa aagaagattt gattgctgtc ctggaaaacg tcaggaaatg
     3481 ggaccgagtg gacgcaaaca acatattcgt gttcggaatg agccagggcg gagcagtcgc
     3541 ggcactgaca gccgatgagc acggtgcgga tataaagggc ctggttttgg actttcctgc
     3601 gctttgcatt ccggatgact ggaacaaacg gttcccgaca cgagagagca tacccgatct
     3661 gtacgaattc tggggagtga cgcttggcag ggcgtatttt gaagcggtcc acggatacga
     3721 catttttgaa catataggga aatatgaccg gcaagtgctg atattccacg gcgacaagga
     3781 taatgtcgta cctgtcggat atggacagag agcggcttca ctatatccca atgcaaaaat
     3841 tgaagtatac cagggagaag ggcatggctt ttcagcaccc tatcactggc gcgtgataga
     3901 gaagacttat gatttggtta tgcagaataa atgaaacgtg ctgccaggca catcagccgg
     3961 aagggcggtt gagacgctgg aacggatctc gtgcctggca accggcaaag gaggataaaa
     4021 atggccaatc catacctgcc gaactgggaa tacataccgg acggtgaacc aagagttttc
     4081 ggaaacaggg tctatgttta cggttcacat gacaacgcag catccggcaa gttttgcgac
     4141 tataaactga aagtctggtc tgcggacgtg aacgatctga ataactggat atgccacgga
     4201 gactcgttcc atacaagggc ggaccgcgac catgaaagcg atacgccatg gacagacggc
     4261 gaactgtatg cgccggatgt agtcgagaaa gacgggaagt actaccttta tgcgtatatt
     4321 ttctatacaa aaggctgcgt tgccgtgagc gacaggccgg aaggaccgtt cagactgatt
     4381 tcacagtaca gcgttcctgc cgtctacgag aacgatatgg ataaaggggt cttctgcgat
     4441 ccgggcgtat tggtggatga tgacggcaga gtctatgttt actggggatt cgaaaggtca
     4501 tacatggctg agatagatcc ggacaacatg catgagatcc tgcccggatc ggtcaggaag
     4561 gatataatcc cggcaggaaa caggccgagc gatttttatg aggcatgttc gccgagaaag
     4621 atcggagata cgtattacct gatctactcg cccaggaaag gaagccggct ggactatgcg
     4681 gtcagcaggt cgccaatggg cccgttcgaa tacggcggca ccatcatcga taatggtatc
     4741 gattatcctg gcgggaacaa ccatggctcc atatgcaaaa taaacggcca gtggtatatt
     4801 ttctatcaca ggatgaccaa caattctgtc ttttcaaggc gtgcctgcgt cgaacgcatt
     4861 gagatcctca gcgacggaag gatcccgcag gtcgagatga catcactggg ttttgaaaag
     4921 tctctttccc cgtaccgata tacgccggct gacattgcat gcgtgctgaa aggcggatgc
     4981 tacataactg agaggaacat tttccagcgg cccgtagtca atattacgaa tggatgtgtc
     5041 gtcggcttta aatattttga tttcggagaa gattactcca gcaggaccat ggaattctcg
     5101 gccaaaatca ggggctgcgg ctcggaagga caggtcagga tcctcatcga cgactggaaa
     5161 gacggcatgg aaattggcag atgcgatatt ggcccggatg acgggatcct ttattcagtc
     5221 gtgcggtgcg tgactggaag gcacgcattg tatttcgttt tcgaggacaa atacggcgac
     5281 gtctgggagt gggcaaaagg aggcttcgaa aacaggccga tctgcgaact ggagggtttt
     5341 gtgttcagca aataatgctg caggacgcgt caaacagagg gatggctgag atacggggac
     5401 ggttttcctg cccggtgtac ccggcagcca taagggaagt cgatatgcac tttaaggaag
     5461 ccaaatcgat attgtcgtca cagaacggta tgaatatata ccgtggctgc agccatggct
     5521 gcatttactg tgacagcagg agcaaatgct acaacatgga gcatgaattc gaagacatag
     5581 aagtcaaggt caacgcgcct gaactgctgg aagctgcgct gaaaaagaag cgcaaaaagt
     5641 gcatgataag cacaggcagc atgtgtgatc catatatgca catcgaagaa aagctgggct
     5701 atatgagaaa gtgtctcgag atcatagaca aatatggttt cggcgttacc gtcctgacaa
     5761 aatccaaccg catactcagg gacctggacc ttttgacaag cataaacagg agatccaaat
     5821 gtgttgtcca gatgacgctt actacatacg acgaaaacct ctgcaggatc ctggagcctg
     5881 ctgtctgtac aaccagggaa cgttttgagg tgctgaagat catgaatcag aacggtattc
     5941 caacggtcgt atggctggac ccgatcctgc ctttcataaa cgatacggag gagaatctga
     6001 gaggcatcct ggactactgc gccaagtcga aagtatatgg gatcatatgt tttggcatgg
     6061 gtatgacatt gcgcgaaggc aacagggaat acttttattc gaaactcgat gtacattttc
     6121 cgggtcttaa gcagaagtac cataaaaaat acggttacag ttacgaagta acgagcgaca
     6181 acaacgataa actaatggac attttttaca gagagtgcag aaaacacggc atagtctgcg
     6241 ataatgccaa gattttcaga tacctgcata catacgaggt caggcagccg gagcaattga
     6301 gctttttcga ttgagatacg ggaacattca ggcatgggac agtacgtgat atttttaatg
     6361 ataacattat attcacttgc ggcgtgacaa ctgcagggat aaaatcaaac aaacggtata
     6421 tcccgggtgc tacggcatcc ggagcgactt atcgacgtaa ggaatgatcc gatatgtacc
     6481 tggaattatg tgcgttgttg aaaaaacctg atctgtatga gagaacacac gagaagttct
     6541 ggaatgatcc gcatatatcc aaagggatgc tggaagccca tctggatccc aacaccgacg
     6601 ctgcgagccg caggcccgag ttcatcgacc ggtctgtcga atggatcatt tccctgctgc
     6661 cggaaggggc aagcctgctg gatatcggct gcggacccgg gctatatacg aagcgctttg
     6721 cggacagggg attcagcgtc acgggcatgg atctctcgga gcattccatt tcctatgccc
     6781 gggaacatga caaaaagagc aattatgttt ttatggatta cctgcagatg gactacgaaa
     6841 atgccttcga tatgatcaca cttatttatt gcgactatgg agcgctgata cccgttgagc
     6901 gcgccggcct gctgcagcgc gtttaccggg cgctgaagcc gggaggcagg ttccttttcg
     6961 atgttttcac gccagtctac acctcaggca tacaagaagg cacatcatgg gaaatctgcc
     7021 ggcagggcgg attctggagc gatgaaccct atatctgcct gaacgcgcaa tatcgctata
     7081 atgaaaacat ctctctcgac aggtatgtta ttataaaaga gaacgcggtg cactgctaca
     7141 atatatggga caccagcttc acaacggact tccttctgag cgaggtacta ccggcaggat
     7201 tcatatatga cgggttttat gccgatgtgg ccggaaaccc ttattacgaa ggatcggaaa
     7261 cattatgcgc tgtgttcagg aaataaaaat ggtacctggc acggcggcag tcacttatta
     7321 taaagagtat tggacaattg gcggccgaat gtcgcttcat taagactgga ggattggaat
     7381 ggcgaaaccg gacatcaccc tgaggcttga acgccccgaa gattaccatg ctgttgagga
     7441 actgacgcgc gaagcgttct ggagaagtat ccgaggtttc tgtgacgaac atctgcttgt
     7501 gcacaggctg cgcaaagtcc cggtttttat cccggaactt gactatgtgg ctgaggcgga
     7561 cggcaggatc gtgggcaaca ttatttatac gagggccagg atcgaggatc catccgggat
     7621 aacacacgag gtgctcacgt tcggacctct cagcgtgctg ccggaatacc agaacatggg
     7681 ggtcggcaaa gccctgatgc tgcatacgtt tgagaaagcc agaaaacttg gttaccgtgc
     7741 catagtgata ttcggacatc ctgattatta tccccgcgtc gggttcaggc gggcgtcaga
     7801 gtttggcctt accacctccg acgggaatac tttcgatgcc ttcatggcgc tgccgctgta
     7861 tgagggagcg cttgacggca tccagggacg ttttttcatc gatccggttt ttgagagcct
     7921 tgacgataag gatgtgctgg agtttgacaa gagcttcccg ccaaaggaca ggtatgtccc
     7981 ggtccccata gaagtattgc ttgaccgcct tgatgccggc gcaagggaag ctgtcgaagg
     8041 actgggctgc acttatcttg acgaatttac ccacagaagc gagcgtgata tttcatctgc
     8101 aacgggtctg gatgaaaagg cgatggacat agtccgccag gttatgctgg aacatggctg
     8161 gcggtgggga tcaaaataaa agaatggtgt tgaaagcgag ggaaagtagt gcggcaggat
     8221 ttcacaggtg taacgaagga ggaactttct gaactgtttc cggtaaaact cgagaagcat
     8281 gatcccgcat ggaaggatca ttatgcggct gaagcggatt tcctgcggtc tgttttcggc
     8341 gataannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccca actggcccgt
     8461 gcagaattcc cgggcagata tgcaccatag caaacatatg gtaagcgaaa ataccgttgt
     8521 gaagctcagg gacccgatcc cattcagttg gcgatgacga cattaaggca tctgttaatc
     8581 cctccgtctt accggtatgt gaatatcagg acataatatc ccggtgatat taccgggata
     8641 ttacatttat gttacgaatc aaaatcacct cggaatattt cacaatgccg gcgaatctat
     8701 attacaaaag cagaaatgta ttataaaacc agaaatgtat tataaaacca atggagggag
     8761 aaatggtatg aaaaacggga taagaaagct tatttcagtt gttctgtgtt ttacactgct
     8821 tttctctgtt tcgtctttgg cttttgcaaa gtcagcgcag tcgaactcca aacagttcaa
     8881 agacgtaaaa ccaggccact gggcatatga tgccatcatg tggatgctgg aaaggagcat
     8941 catcgacggc gtcggaggcg accgttttga tccgaacggt atagttacca gggcacagtt
     9001 tgccaagatg atggtaaaca ctctcaatct gaaaaaatac tccccggaaa cgccttcgtt
     9061 cctggatgta aagaagaatt cgtgggagta cccgtatgtg gaaagcgcca agccatacct
     9121 cacaggcttc aggacttcca gcggagatta cttcaaaccg tcgctggcgg cggtcagaga
     9181 agacatggct gttgcacttg tcaatgcact tggctatcag ggtgagtcaa tagatacaag
     9241 tatccttaac cagttcgcag acaaggacca gatctctccg aacctcagga agcacgttgc
     9301 attgtcggtc aagtacggcc ttattgtggg gaaaacggaa aacggcaggc tgctgttcga
     9361 tccgcagggc aatctgacgc gtgcacaggc ggccgttctt ctgaagcgtg catttgagaa
     9421 gaacgaagag aaagtgacat atgatgaaga aaaagtcacc tatgacccga acaccggtta
     9481 tacgtacgag aggccgataa tagaactcaa aacggaaaac aatgtgcagg tgatcaggtg
     9541 gaaccggata gagtctgata aattcaggga gtacagggtc gtgatctcta aaaacgatga
     9601 tactccccag tacccggata atggttacct ctacacaatc acggacagga acaggacata
     9661 tgccgttatc gacaacaccg ataagtacaa tggcggcgat ttcggcaatt acctcaccaa
     9721 agggcagacc tattatataa gcgtgacggc ggtatactcc gacaggaatg tgccggggaa
     9781 cacggtcagc tttgtctata acggtcctga gaaccctgaa ctgtacgtgg cgcctgatgt
     9841 cagttcttcc gtggagaacg ggaaactggt gttgagatgg aacaggatcg attcgcccaa
     9901 gctgacagga tatattgttt cggcatccaa gaatgacagc actgtaacat acccggagaa
     9961 cggctacctg tataagatca atgatgtaaa caggacttac gcggtgatag ataactccac
    10021 caggtatacc gacggcgatt tcgaaggcta ttttatcaat ggtgagcagt attacttcag
    10081 tgtgacggct gtttacaatg ataagtcggt gaccggcaac accataaggt gcacgtatgt
    10141 cggcgaagac agcccggaac tgttcccggc gccgcaggtg agcgcagcgt acgaagacgg
    10201 caagctggtg gtgaagtgga acaagctgga ttcttcgctt ctgacggaat accgtgttgt
    10261 catctcggag aaaagcgata aacccgcata tcctgcaaac ggctattatg atcaggctta
    10321 tggtaaagat accacatcgg ttgtcatcga ctctgccaaa cagtatcaca acggagattt
    10381 tgaaaaactc acctatgcaa ctgagtatta tataagcgta acagcggtat atggcaataa
    10441 atatgtcgca gggaatgcgg tcaagatcct ttacctgata aatgaccagc aggcggaata
    10501 acagcaaagg cggtacggat tcgaaaacag gaagcggcag aaaggaatgc agcaggcgag
    10561 gggcaaaaaa gcgtccctcg tttgctgttt ttacttgaca ctttctgatt tccaaataca
    10621 atgatagtat gttaattatt gcattaaacc taaatggata caaacagacg ggatgaggta
    10681 attatgggcg gtatattcgg tgttgtctca aaaaacgact gtgtaatgga tctgtatttc
    10741 ggaactgatt accattcgca tctgggtacg agcaggggcg gaatggcggt gttttccgga
    10801 aacggatttt ccggatatat acacaatatc gagaatattc aattccgggc gaaattcgag
    10861 agcgatcttg ccgagatgtc cggaaacatg ggcataggat gcatcagcga taccgaggcg
    10921 cagccgctta cggtgtgctc ccaccatgga cactatgcgc tgacgaccgt cggtcgcgta
    10981 aacaacatcg acgaactggt caaaaaggct tttcaacgca attatataca ttttctcgag
    11041 atgagcaggg gcgagataaa ccccacggaa ctggtcgccg ctctcatcga tcaggaagaa
    11101 accatcaagg atggtatcct tggagcgcag gaagcaatag acggttcgtg tacgatgctc
    11161 gtccttaccc atgaaggcat ctatgctgca agggaccggt tgggcaggac gccgctgctg
    11221 gtggggaaaa aggacgaatc gtactgtgtt tcgcttgaat ccagtgcgct tccgaacctt
    11281 gggtacagcc tggaatatga actggggccg ggcgagataa ttttgctgac gcctgaaggg
    11341 atcgtgccct tggcgccgcc aaggcaggaa atgaagatat gcgcgttcct gtgggtctat
    11401 tacggctacc ctgcgtccac ctatgagggc aggaatgtgg agataatgag gtacaaatgc
    11461 gggcaggccc ttgccagggg cgacaccagg gaggtggatg ccgtagcggg agtccctgat
    11521 tccggcattg cacacgcgct gggttattcc aatgaatcag gcataccgta ttccaggcct
    11581 cttgttaagt atactccgac atggtcaaga agtttcatac cccaggagca gagtgtccgg
    11641 agccttgttg caaaaatgaa actcatcccg ataccggaac ttatcaggaa taagcggctg
    11701 cttatctgtg acgactccat agtccgcggg acacaactgg gacagactgt agcgcttctg
    11761 cattacttcg atgccaggga agtacatgtc aggccggcgt gcccgccgct gctgttcggg
    11821 tgcaaatacc tcaacttttc aagatcgcgt tcggaaatgg acctgatcac acgacgttcc
    11881 atagtggaga tcgaaggccg tgagccctcc ggctttgatg agtactgtga tcccaaaacc
    11941 gaaaaatacc attgcatggt ggagtgcatc agaaagaaat tgagttttac aagcctcaga
    12001 ttccaaaagc ttgaggatat gatcgatgcc ataggccttc cggcgtgcaa ggtctgcaca
    12061 tattgctgga acggcaggga atgatccgca atgaccggat caaggctgta atttatattc
    12121 agtccacatc aggcacgact ttcaccggag ggacttccag cttttcaggg ctctcgatca
    12181 gagttttgta aagcttgact gctgaggcga aatagtggcc gtcgacgatc ctttcatcac
    12241 acactatcct catcgttata aaccttctgt tgacaatgtt gttgtccgag tctatgactt
    12301 tttcggtcct tttcgtgcca aatgagacga atacggatgt cgtaccgaaa tcataaaggt
    12361 gatgatatac aggagaaata cccagtgatc cgacatcggt tataaaaacg cttgtatgga
    12421 aaggacttgc cttgtatatg atctttggca taagcccgaa gtaatccagg agtctgacca
    12481 cgaacactat gaatttcacg atgaagccgg gacaaagcgt aacgatgcgc gcagtcttat
    12541 ctgtcgtgtt gtcggattcc gcttccttgt tttcctttac ctgttgctct actttcctgg
    12601 ctacatcata caatgtatct gtcggttcga atttgaatat tatggtcgtt tcagggctgt
    12661 cgaaagtcat ttccttcttt attgcaaggg ataccaggta ttcaaagcgg gcaaaaagtt
    12721 tctggcctgc gacgaacctg ttgagagcgg gtctcagggc aagagttctg acggcagcag
    12781 caataaagat atggagcatg ctcagcctcg tatcggaggt tttgttcctt ttccttaaat
    12841 atgcctctgc atgtgtgata tcgatttttt cttcgaagaa tatctgcgag tccaaacggc
    12901 tccgcatgat gtaaggtatt atcttgaaga aaggatccag agatctcaga cgcctgccgt
    12961 cgtatctgtc gccaaaccgc cttttgtaac ctgccatcag atgaaaacct cctgtaaaat
    13021 acaagttatt tgatttatgt tgcacgattc ctttctagtt taacattgct tcgacagatt
    13081 tacaatgcat tacggcacat ttggacagtt tcaacaatat gtaaactcaa cagccaactc
    13141 gtgcggaacg taaatgttgt tataacaagc tactaaaata tccaggttat aaaacaatca
    13201 ttcaatgcca tatatcagcg gagcacagtt gacgtgctta tatgcttctg ctatcatagt
    13261 aataacatgt tataacgtta taacacttat tttgagtaat tttatacata ttgtacaggc
    13321 gaggttcagc cggaaaccct gatctgcgcc atataatacg aaaggtggaa acgacatggt
    13381 actgacagtg aatgatattg cacagatgtt tgatctaagc tgtgtcagga cgaacagcga
    13441 tgaaaaagat atcaaagacc tggcagagaa cgccagacgt tataattgcg gacatgtgtc
    13501 cgtcatgcag tgctttattc cggtcataaa gaaactgctt tcggacaggc ccgatataaa
    13561 agtagtcggg aatgtgagct tcccgtccgg gtccgacaca acaagcgtaa aggtgttcca
    13621 ggcagttgaa ctgagatatg ccggctgtga tgagatagat atggtgataa atgtgggcaa
    13681 gctgcgttca ggaagatacg cggaagtcga agatgatgta aaggcagtgg tggaagctgc
    13741 cgacaggctg ccggtaaagg ccattatcga agtatcgtgc cttgacagga aagaagtgga
    13801 gaaagcctgt gaaataagca taaaggcagg cgctgcgttt attaaaaccg gcacggggtg
    13861 gatgcccggg ggtacgacga ttgaagatat aaagctcata aaatcgatag tgggcgaccg
    13921 tatcaggatc aaggcatcag gaggaataag gaccctcgat acgctgttgg agatgtacag
    13981 tctcggggtc acccggttcg gcgtgaatat gtgcagcggc atcgcgatac tcgacgagtg
    14041 cattgccagg ggcggcgagg ttacaatata agcagaagac ggtcacgata agcagaaggt
    14101 ggtcacgttg accgtgtatg aagcggcttt gaggaaaaag agcaaatcag catggatccg
    14161 ggccataatc caagtatatg aatgcggcat cagatttcga tataggcgag aggtggcaga
    14221 atgggaaaca ggattatctc atatgatctc ggcacaggcg gcaacaaagc ttcgctgtac
    14281 gactttgaag gcaattgcct ggcatcggta ttcatcccgt atgatacctt atatccccga
    14341 gcgggatggc acgagcagcg gccggaggac tggtggcacg ctgttgtgct cggcacaagg
    14401 aaactgctcg aaaagacagg cgaggaccgc aaaaatatcg aatgcctggc tatttccggg
    14461 catagccttg gcgccgtacc tgttgataaa aatgggcgcc ttctcagaaa aacggcctcg
    14521 atatggtcgg atgccagggc ccaggagcag gcacgtaaat ttttcgcgaa tgtcgatgag
    14581 cacgaatggt acatgacgac cggaaacggc tttcccgccg cattgtatgc tgtttttaaa
    14641 ataatgtggt acatggacaa tgaaccggat atatacaggg ataccttcaa aattattggg
    14701 accaaggatt acattaacat gaagctgacc ggcgtcgtat gtacagacaa ttcatatgcc
    14761 tcagggtcag gcgtatacga tctgaaatgg aacaggtacc atgatgggta tatcggtgca
    14821 agcggcatat ccgccgacaa actgcccgaa atagtgccct cctccgagat tttgggaact
    14881 gtaacgccgg aggcatccga agaaacggga cttgggacga atgccagggt agtctgcggc
    14941 ggcgtcgaca actcatgcat ggcattgggc gcgaaaaacc tggaagaggg cagggtatac
    15001 acctccctcg gttcgtcggc atggatcgcc gtatcttccg aagaccccat acttgacctg
    15061 aaagcaaggc cttatgtgtt tgcccatgtc atacccggaa tgtacacttc cgccgtcagc
    15121 atcttctctg cgggtacttc gttcagatgg ctcagggaca acatatgcgc cgatctgaaa
    15181 atgcaggaac tggatggcgg gaaaaacacg tatgcgataa tggatgaact ggcggagaaa
    15241 tctcctgtgg gagcaaacag gctgctgttc aacccaagcc tggcaggagg cacttcacag
    15301 gatgccagcg ccaatctgag aggagccttt atgggactgg acctgaagca tgacagggca
    15361 gatcttatcc gtgcatgtat ggaggggatc gcgatgaacc tcaggctgag gctggatgtg
    15421 ctgaaaaagc actgcaggct ccaaaacgag atgctgctgg tgggaggagg aagcaaaagt
    15481 ccgctgtaca ggaggatatt tgctgacgtt tataatatgg atatcgtgaa gaccaacata
    15541 gaccaggatg ccgctgctct tggcgctgct ggcgtggcgg cggtcggatg cggcatatgg
    15601 aaagactttt ccaggatcga tgagatacac aggacgacag agatcatgaa gcctgatcct
    15661 gcaaataacg ccaaatacga gaagatcctt gaagtattca gctttgcttc ggacaggcag
    15721 tcggagatct ccgacaggct ttttgcactg gaactgtgat gcaatgcaag atttatccgg
    15781 tttgttttct gtctctgcac ctgtttgatt tatgtctact gtacttggat gtcaaaatat
    15841 tatataattg gaataaaaat aacctttaag acataagggg tgaagaactt gcttgataaa
    15901 tctcgcccta ttcctctgta ttatcaggta aaggagagcc tgctggagaa aataaagagc
    15961 aggcagttca atgtaggcga tcttataccg tcggaggcag aactgcagga gcaatacaat
    16021 gtaagccgga tcacggtcag gcgggcgata caggaactgg tacaggaagg acatctgtat
    16081 accaaacagg gcagggggac tttcgtaagc aggcctaaag tcagccagga actcaatctt
    16141 ataacaagtt gggctgagac catggaagag atgggtatgc gcccggaaac aaagaaaatt
    16201 gagtattctg aggagccggc tcctgtaaat atagccaggc tcctgaacgt accgatcggc
    16261 gacatggtgt ataaaatcga aagattgcgt tatgcggacg gcgaaccgac atgtatcatg
    16321 acgaactacc tttctcctga cgtggttccc ggtcttttgg ataaagggat aaaaggcgaa
    16381 tctttatacg aaacgctcga gaagcattac aatatagtat tgagccatgc cgaggagacg
    16441 gtggaagcga aggcggcgaa aacctcggaa gctgaactgc tcaatatcaa aagaggcgct
    16501 cctttgcttc atgcaacgag ggtgacatat gatataaccg accgtcccgt ggaagtagtt
    16561 atatcgattt ccagggcgga ccgctattca tacaaaatca ggctcagcgg caggaaaaag
    16621 acgatctgag caaggatacg gcattgagta atggcataat atgcccgaca gtattatttg
    16681 tcgtggcgta ttatgctgat cagaatcagg cttgcgtgat gtcctgttat gccattgctg
    16741 tgattgtcat gtccaaaatt tcggccaggt tcaattctca atgagcattc tatataatta
    16801 cgctataatt acgttttgtt ttgtcacaaa aattactgcc atgaaaaagg gactacccct
    16861 tgatcagatt tttctgattt tgagacagcc cctttttatc actcatttgg atatttattt
    16921 ttcttattct taagcctctg cattttcagg ggtttgcttt ttacccgtct ttttatctaa
    16981 ggatctttct ttcagtggcg gaaggatcga cgaatacctt gatcagatcc ttgttcgtcc
    17041 tgagttcgtg aagagcctgt tccagttctt ccagcggaac gatcatcgag aacatgggag
    17101 tcggatctat gcgcttgtat ttgagcagtg tcagcacgtc tccccagtca ttccctatac
    17161 cggcgcatga cccgctgacc tttttctcgc cgagtacgaa ttcgtaaggg gagaagcagg
    17221 ctttccggtc gggcgggcat ataccgaagg cttcgacctt accgcccctt ctgaccatgc
    17281 tgaatgcctg ctcatatgtt tcgggggttc cgaccgactc cagtacgtaa tcggctccta
    17341 cgcctccggt gagtcttttt acttccgcca cgggatcgtc cacctcgttg atgtttatcg
    17401 taaagtcagc gccgagctgc tttgccagtc tgagcttgga ttcgcttctt ccgatcacga
    17461 tgacgggagc ggcgccgcgc agtttcgcaa gggcgccgtg caggatgccg aggcctgtgc
    17521 ctatgacaac aacgctggaa gcaatcgcga catccatctt ctttgcagcc tgcatgaccg
    17581 cagtgagagg ttcgacaaat gctgccgaca gcgcatccac ttcatcaggt atgaggtagc
    17641 agttcgtcca gggcgctttt acatactctg caaatgctcc gtccgtatgg atcccaagct
    17701 gcgtaaaggt ctcgcagagg agttcgtcgc ctctgcggca gtaatagcag gttccgcagg
    17761 gaaggcatat atcgactgcg accctgtcgc cttttttcag gcctcttgcg ttgctgccca
    17821 cttcttcgat aacaccccag aattcgtgcc ccgatatcat cggcaggaat tcggaagcgt
    17881 attcaccttt gtatattttc cagtcagtgc cgcatatgcc gcacatggag accttgataa
    17941 gcacttcgtc tggagatatt tcaggtaccg ggatctgttt gaggaccatt ttgcccggcg
    18001 cttcgataac ctgggctctc atcattcttg ccatggatat tacctccgta atttagaatt
    18061 atacagctta cagaacgtcc ttccccttga agagcttatc ctggcggcct tcatacgggg
    18121 caccgatcag gaggggttta aggaatgcaa ctgtcttgac tacgccatcc atacggctca
    18181 tggtgacgtc ttcgtgctca tagctgagta cgtaattata tcctaccatc gacagttcag
    18241 ttatgacttt cttccacggc acgctgcccc agccggggat cctgaacctg aatgcgcggt
    18301 cgagcctttg ccattctccc tgcataagta ttccgccgac cggaaggtta tgctccacga
    18361 tctccccgtc ctttgcatgt acatggaata tcctgtcgcc aagcctgtct atgaagttct
    18421 cgactctgag gccgaggtaa atcaggtttg cggggtccag attgaatccg aggcatggat
    18481 ggtaatcaac cagtttgagg gctttttccg cggtatggat atcgtagatg aagttgttcg
    18541 gatggggttc taccgcaaat ttgacgccat attccttaaa tttgtccagt atcgggacaa
    18601 agagttccac gaattctttt tctatatcag cccagccctg ggcatagggg aacgggaagt
    18661 acctgccgaa attcgttact cctgtaaatc cgttaactac aggaacttcc agggcatttg
    18721 cagcctgggc cgtcctgagc aggctttcgg ttccgaatct gatcttctct tccttagtgc
    18781 ccttataaat ggtgtccgta tcgacgccgt ggggcccgag gatcaggaga gaatctgcat
    18841 ggttgctcag tgcagatatc gtcaggccgt aaccttcgac catctttttg atttttttgg
    18901 cattgtcgcc ttccaggact tccctgggct cgaattgacc attgccttcg taagccggga
    18961 tctccagtgc ttcataaccg tgttctgctg ccagttttgc tacttcttca agcggtcttt
    19021 cggtgaaatt tgctgtgaag aatcctagtt tcattagatc agcctccgtt tcaagttata
    19081 tgtagttagc attacctgaa tatgctatgt ttattttagc ccgaaaatgt tgaaatgtca
    19141 acgaaattac atataataaa aggtggagtg tgtaaaaatg tttgtttatg atttttacag
    19201 cttgaaatcg gaaattgcat atgatattgt tattatgatg ttataacgag gcgataaaat
    19261 ttcccgcttg tattccactt gcctcgttga aacgcgcagg ggcaggaaaa atttctgcaa
    19321 tcgaaatttt tctttgatgc gttatatcat gtgcagcata tttttatcag aggaggcaat
    19381 tcttatggat tacaaaaaaa cacttctatc gcctgttaaa ataggtacga ggataagccc
    19441 gaaccgtttt ttcatccagg caatggaatg tactgatgct gacaaggacg gcaatccctc
    19501 agacctgact tacaaacgct atgagaatct gttccgcggc gaagccggag tcgtaacgct
    19561 tgaagcgatt tctatcactg ataaaagcag gtcgaggcag gaacagctcc tgatcatgcc
    19621 caggaatgag aaggcgcttg ctaacttcgt caaaaggatg aaagaaatca atcctaattc
    19681 gttgttcctt ttccagctca cccattcggg cgaactgagc catccggact tctcgcgccg
    19741 cgtgaccgta gagcctttgc cgggcttcgg cggcgaattg ctcagcgaag aggaagtcga
    19801 ccggatcatg gatcagttcg tgctggccgt
//
LOCUS       DAOSCC010000015        17657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2221_length_17657_cov_4.839223, whole genome shotgun sequence.
ACCESSION   DAOSCC010000015 DAOSCC010000000
VERSION     DAOSCC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17657
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_17657_cov_4.839223"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..725
                     /locus_tag="PLG72_01755"
     CDS             75..725
                     /locus_tag="PLG72_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ79560.1"
                     /translation="MMESRISVLIADDHPLFRQGIKQILELEKDIIVVAQAANGDDAV
                     QLAREHKPDVILMDINMPGSNGLQAIKEIKDENISSKIIVLTIHEDREYLIKTIQMGA
                     EGYVLKDAEPQVLIDAIRNVHIGQSYIQPNMTKELVMEFNRLTLHNKDRNGENRLTAR
                     EREVLKLIAEGMINKEIATKLYISEKTVKNHVSSIFRKLNVSDRTQAAIYAIKHNI"
     gene            1370..1576
                     /locus_tag="PLG72_01760"
     CDS             1370..1576
                     /locus_tag="PLG72_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ79561.1"
                     /translation="MKVKEFMTKLMVRFQSRKGQGMVEYGLIIGIIAVILVASLTVLR
                     APLTTLFGNIRDIITDNTPAVPAP"
     gene            1807..2211
                     /locus_tag="PLG72_01765"
     CDS             1807..2211
                     /locus_tag="PLG72_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ79562.1"
                     /translation="MVRNRNKTGKRSGQSLVETALVLPLLLLILMGIIDFGLLFNSYL
                     VVSNASRDGARHAVTGDTDAQIRAAVCSAASTLDLTRLDITISPDEGEGRSTGDSVTV
                     TVKFEYSMVTPIIGAFFPDTFDIESSTTMRCE"
     gene            2226..3005
                     /gene="cpaB"
                     /locus_tag="PLG72_01770"
     CDS             2226..3005
                     /gene="cpaB"
                     /locus_tag="PLG72_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOJ79563.1"
                     /translation="MEKKNRKMLIIALTLSLITAALIYIYISGIKPEQAPEKEYTKVC
                     TAARTITEFTQITAPDIAEVEVDKELYREGMVTDKEQIIGKWALQDIVKDEFIRKERI
                     GDEESLNFSYYIPEGTRAVSMNVTEQVNAANLLRPGDYVDVIASFNKEEEVYGERTVT
                     YPKITKIILQNVRVLALGQDRKLPAEKLQEQPATITLAVKKEDVEKFVYAAEYGIIRL
                     ALRPAGDNSRTDSQGAIREDVTGTKGVIVTDNADGSAGASE"
     gene            3223..3990
                     /locus_tag="PLG72_01775"
     CDS             3223..3990
                     /locus_tag="PLG72_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79564.1"
                     /translation="MEKINVMVVDDSYETRSNIKQLLSFSKNIKVAAEAGSGEEAIML
                     ANEMKPDVILMDINMPGIDGIKATEEISLNVPDSVVIIMSVQGETEYVRKAMAAGARD
                     YLCKPFGGDELSETIIKTYENEMRRREKIAPVTAKEEIRSRVITVFSTKGGVGKTTIA
                     SNLAVSIARSEKKRVALLDFDLQFGDVAIMLNVSVKNTISDLVKEFGQLDESIVEDYL
                     VTHFSGVRVLPAPAKPEYAEYITANHVEKIINILRDS"
     gene            4051..4419
                     /locus_tag="PLG72_01780"
     CDS             4051..4419
                     /locus_tag="PLG72_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79565.1"
                     /translation="MSDKILVISTLDLPTIKNVKSGLDVMETLNYPQDKIHVVLNKAS
                     EQFGIRYRDFEDVLHKQIWSYVPEDSQTVVTSANKGFPFVMTRTETKVAKAIIDMGNE
                     LMMELEGGVNKKPPLRKLFG"
     gene            4435..5802
                     /locus_tag="PLG72_01785"
     CDS             4435..5802
                     /locus_tag="PLG72_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOJ79566.1"
                     /translation="MSLLERLQKERAVETSESGKSQGTRNVQAPKEDPFEDLKVRIHM
                     RIIEEINSGTVRDVEAEGKESDLEQAISGIVESYLDEEGVFITKLDKQKLITEIIDET
                     IGFGPINRLIRDPSISEIMVNGPQNVYVEKKGRLIRTDVVFKDDQHVMKVIEKIVAPL
                     GRRIDESSPMVDARLPDGSRVNAIIPPLALNGPSLTIRKFSERPFTVKDLINFGTMTP
                     EVAMFLKACVEARLNIVVSGGTGSGKTTTLNVISSFIPDDERIITIEDAAELQLAQEH
                     VVRLETRPPNIEGKGAITIRDLVRNSLRMRPDRIVVGEVRSGEALDMLQAMNTGHDGS
                     LTTGHANSPRDMLSRLETMVLMAGMDLPVRAIREQISSAIDLIVQQARLKDGSRKITH
                     ITEVTGMEGDVITMQDIFIFKQHGRDEKGRIIGSIVPTGIKPKFVEKLADAGISLPPD
                     IFVAK"
     gene            5893..6864
                     /locus_tag="PLG72_01790"
     CDS             5893..6864
                     /locus_tag="PLG72_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ79567.1"
                     /translation="MIGLIILMSFISAAVLYYQLLMLFSSGKDYMARLRRYTAPEGMY
                     EEKMKKRRDSSSLLRDVLSKGIGKIKFLDGYKKRIQADLARAHLLLKAEEFLVFEVIL
                     FSLFFLLTILIKGTRSWILAIIFGIAGWILPSFLLKNRIKKRMKLLNEQLGDAITMMS
                     NSLKAGYSFLQTVDIVAEEMTGPVSEEFAIFSKEVSLGLNTEKALENLSARVRSEDLD
                     LAVTAVMIQRQVGGNLAEVLDKITETIRDRVRIKGELKAVTAQGRTSGLVISLLPVVL
                     CLIIYMINPAQMSLLFTRPLGIIMVAVAAVMELTGIMLIRRIVRIEI"
     gene            6878..7798
                     /locus_tag="PLG72_01795"
     CDS             6878..7798
                     /locus_tag="PLG72_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ79568.1"
                     /translation="MPVLIILLAFASCFLLFYTVLSIAGSDELIISSRLKSIKDSGTK
                     KEDGELDQPLLNRVIRPMLDHLGKVMMRITPKEMISSLEYKITRAGTPWNLSVRDWVN
                     IQAFLVAGIPLVTFIALKKSGVPGGTILLFLISEAALGFALPGFILGKMLTARQKAVL
                     NSLPDVIDLITVIVEAGLGFDGALMKVVEKKPGPLAEEFDKVLQEIKVGRNKKDALRD
                     MAQRLDIHDFTAFVGAIIQAEQFGVGIAQVLRIQSEQIRLKRKQRAQERAMKVPVRML
                     IPMVMFIFPTLFVVLLGPVVIRLIEQFSGM"
     gene            7986..9290
                     /locus_tag="PLG72_01800"
     CDS             7986..9290
                     /locus_tag="PLG72_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ79569.1"
                     /translation="MGFLDDIRNKILIHDGSKGYMLQRMGLKGGECGEYWNLTNQDAV
                     RAIYRAYLEAGSDVLQTNTFTGNRIHLGRFGLGDRTYEINYWGTRLAKEVAGDRAYVS
                     ASVGPTGSLMEPLGNLSFESACEVYREQVKALADGGADVINFETFTDLSELRAAYLAA
                     RDVTDLPVICSLAFETNGRTLMGTDPFIAVNVLKSMGADMVGANCSFGPEHMKGIIEG
                     MYKAGGGYLSVKPNAGLPSVSGNAVKYHETPEHFADLSAEFVKFGARLIGGCCGTTPD
                     FIKALKNKLSETQPVTVAGRTAICITSDVKYINVKDLDKANIYRIDAAKDQTLYEALK
                     NNDFSWAEETALDLAAEGYDAILVNTDSAGGDAGLLANVIDRLQWYVKDPFIIETNNA
                     AALERALRIYRGTAGVVINSSSDDELRNIAEKYGSVIISRDI"
     gene            9417..10358
                     /gene="murA"
                     /locus_tag="PLG72_01805"
                     /pseudo
     CDS             9417..10358
                     /gene="murA"
                     /locus_tag="PLG72_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
     gene            10417..11277
                     /locus_tag="PLG72_01810"
     CDS             10417..11277
                     /locus_tag="PLG72_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ79570.1"
                     /translation="MKLRRIKPMQEPAYVINRRKRRKQRIWRAVKSILVTALFVFTVI
                     YASLSPFFNIKDIRVAGSTRYSTAELSTASGIVTGRNGFRVLFSSKSKPGLFRIGDAE
                     QAIIDSCPYVKSAKVRYIIPGTVRIDITEREPAAIIDIKGIQLLIDKEGYLLEIDPVF
                     DAETDESGLPVIRTAEPGSANPGKKLDIPEEMLLSAFKVFDTIKEMDAVNDDKLLPMV
                     DYVDTSDLSNIRLSLQARIVVNLGKADELHYKINAAASIFTRNIKKTERGTLDFSVGK
                     DPVFTPESGG"
     gene            11284..11634
                     /locus_tag="PLG72_01815"
     CDS             11284..11634
                     /locus_tag="PLG72_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HOJ79571.1"
                     /translation="MLPILGMIIGILIGVFLIPVNIPQQYSIYLAVAILAALDSVFGG
                     VASSLQGKFDMKIFLSGFFGNAILAAILAYIGDQMGLQLYLAAIFAFGNRIFLNFAVI
                     RRYMLNKSDKKDNI"
     gene            11666..12898
                     /gene="ftsA"
                     /locus_tag="PLG72_01820"
     CDS             11666..12898
                     /gene="ftsA"
                     /locus_tag="PLG72_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ79572.1"
                     /translation="MSDWIVSIDIGTSNVCALIGKVNKYSQIEIAGRGMAPCSGLKKG
                     VIVDIDSTAASIRKAVEEAETDANMRVDSAYVNIHGMHVNIVNNRSWMSINGREITRD
                     DLDDILRDVRDIDIPEDRQLIDVIPRQFIIDGYDEITDPVGMVGTRLEVDADMLVGKI
                     TSAQNIIKSMERADVKLDGIVAEAFASAEICLTPDERDIGAVLLDVGGSITDVSVFQG
                     KRLVFYDSIPVGGDHITNDIAIGLRISNTEAEKIKRQFELALTSLIKNDQEISVLDIN
                     ENRKKNVKVSEVVEIIEARVYEIFSLCKGLLDKAGINIPREGGVIMTGAGISYVDGCK
                     QLAQEVFDLPVRVVQAGQIPGVLKPEFIASAGMIRYVASVRKGGSRASEIRLRKQEKQ
                     KKDESVFEKILNFIKKMF"
     gene            13009..14106
                     /gene="ftsZ"
                     /locus_tag="PLG72_01825"
     CDS             13009..14106
                     /gene="ftsZ"
                     /locus_tag="PLG72_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ79573.1"
                     /translation="MLEFDIDMEQFAQIKVIGVGGGGNNAVNRMITAGLRGVEFIAIN
                     TDKQALFLSKANTKIQIGDKLTKGLGAGANPEIGEKAANESKDEIAQAIKGADMVFVT
                     AGMGGGTGTGAAPIVAQIAKEMGILTVGVVTKPFMFEGRKRMQYAEQGIENLKGVVDT
                     LVTIPNDRLLQVAEKKTSIIEAFKMADDVLRQGVQGISDLIAVPGLINLDFADVKTIM
                     YNTGLAHMGIGRASGDGRAEEAAKQAIQSPLLETTIDGARGVIINITGGPDLGLFEVN
                     MAAELVQKSADPDANIIVGAVIDENLKDEILITVIATGFDKSPSMKKIDKIAEKSPAK
                     QEQTVPRRQTASDISEDELEIPTFLRKNRFR"
     gene            14664..15569
                     /gene="spoIIGA"
                     /locus_tag="PLG72_01830"
     CDS             14664..15569
                     /gene="spoIIGA"
                     /locus_tag="PLG72_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOJ79574.1"
                     /translation="MEIYLDIVILENIVINYLILLVTSRFSKNRTSSLRLFLGSVAGA
                     AYLVLMILLPDTKVYATLLSKFLLSVGMVAITFSFRKLSVFLKTLALFYATTFIFAGA
                     AFALMFFSKDWGIIRNGVLITPLSFIDAKWSELLLAVAVALIIFRVIWDALQSKFIKE
                     KLLVNISIAMGNKSIELPALVDTGNSLHDPLTKLPVVVVEFSAIKDLLPDDIKSIFEQ
                     NNENDLNSVTSTISSSNWFTRFRLIPFKSLGRENGMMIGFRPDYIEIGADEGKKDIRD
                     VIVGIYNRTLSANEQYRALMNPELI"
     gene            15595..16326
                     /gene="sigE"
                     /locus_tag="PLG72_01835"
     CDS             15595..16326
                     /gene="sigE"
                     /locus_tag="PLG72_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOJ79575.1"
                     /translation="MKVFNKVRLILRIHYWELLARIRRRIGLPIYYIGGSEALPPPLT
                     VDEETYLLARLEEQDYGVKSVLIERNLRLVVYIARKFENTGVGVEDLVSIGTIGLIKA
                     INTFNPSKNIKLATYASRCIENEILMYLRRNNRVKTEISIDEPLNVDWDGNELLLSDI
                     LGTESDIIYRSLEDEIDRELLNTAMKKLSSREKKIMELRFGLTNGVEKTQKEVADMLG
                     ISQSYISRLEKRIISRLKKEINRMV"
     gene            16443..17216
                     /gene="sigG"
                     /locus_tag="PLG72_01840"
     CDS             16443..17216
                     /gene="sigG"
                     /locus_tag="PLG72_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOJ79576.1"
                     /translation="MMVNKVEICGVNTSKLPVLSNEQKKALFERMHKGDASAREEFIN
                     GNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDVSQNVKFSTYAVPMIIGEI
                     RRYLRDNNSIRVSRSLRDIAYKALQAKERLTNKNSKEPTISEIAEELKLPKEEVVFAL
                     DAIQDPISLFESVYHDGGDAIYVMDQVKDEKNIDENWLEGISLKEAMRKLNDREKLIL
                     NLRFFEGRTQMEVAEEIGISQAQVSRLEKTALMHMRKHI"
     gene            17281..17466
                     /locus_tag="PLG72_01845"
     CDS             17281..17466
                     /locus_tag="PLG72_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79577.1"
                     /translation="MKIKRHDNITESINVQTVLTHIGHSKEKNGKVPSEWRTVPEVRE
                     LFPGWRTGPGMEIGTSP"
BASE COUNT         5296 a         3629 c         4626 g         4106 t
ORIGIN      
        1 gagaataagc tccgagccgg ggaaggggac ccggttgaat ataatcatac ctttaacaag
       61 agaagaggag gttaatgatg gagagcagaa ttagtgtcct tattgccgat gaccatccgc
      121 tgttcaggca gggaatcaaa cagatacttg aacttgagaa agatataatt gtcgtggcgc
      181 aggctgccaa cggagacgat gcagtccagc ttgccagaga gcacaaacct gatgttatac
      241 tgatggatat caacatgcca ggctcaaatg gtttgcaggc aatcaaggaa atcaaggatg
      301 aaaacataag cagtaaaatt atcgtactga cgattcacga agacagggaa tacctgatca
      361 agaccattca aatgggagca gagggctatg tgcttaagga tgcagaacca caagtcctga
      421 tcgatgccat aaggaacgtt catataggac agtcctacat ccagcctaac atgacaaagg
      481 agctcgtaat ggagtttaac aggcttactt tgcataacaa ggacagaaac ggcgaaaaca
      541 gactcacggc aagagaacga gaggtgctca aactcatcgc cgaaggaatg ataaataaag
      601 aaatcgccac caagctttac ataagtgaaa aaacggtcaa aaaccatgtt tcgagcatat
      661 tcagaaaact gaacgtttca gacagaacac aagctgcgat ttatgctatc aaacacaaca
      721 tttaagcagc gggtttttta cagtcatatt atccggaaat gtcccgcctg gctgtatatg
      781 ccccggcggc cgtccgttaa ttcgcaaata ttttgggctg ggagtgcctg tcatttacgg
      841 caatcctctc tcagactctc tttcactcag ttctcaaccc ttctatctca aaaagatcat
      901 cagttacact gcataaagtc aatatgaatg ttccctataa tgtttgatca atcatcagca
      961 atcttgataa tcactgtatt caatcaaaca tatctgttaa ttgcatttat cttgtatatg
     1021 atccaatgag ttggacggtc atgtagtgaa ggactgtccc caagaacatt cctgctgaaa
     1081 ttcctgaatc agcataatgg actgtgttga cgtaaattag ttgagaactg tccccaatgc
     1141 acctgcttct ttaccagttg agttggtaag atagtcccag gtgggttgag acttatggga
     1201 taccgcacgg gacactatgg ctcaggctaa cgtttagggc taaataatca gcaaaataag
     1261 ggcttacggt ggattttatc gcaatttatc tcattttata ataaaaccag acaaagttaa
     1321 agggaacgtc aggaactgtt taaccaaatt atgaaaggtg gtacgggaaa tgaaagtaaa
     1381 agaattcatg acaaaactga tggtcaggtt ccagagcaga aaaggccagg gcatggtgga
     1441 gtacggcctg atcataggga tcattgctgt gatcctggtt gcatctttaa cagtcctcag
     1501 agctccgctt acgactcttt ttggcaacat cagggatata atcaccgaca acacacctgc
     1561 agtacccgca ccttgattgt attggcctta acatcctaaa gataaagagt taccctatct
     1621 gtctccaggt agggtaattt tataaaatcc attgaggctt tattgtaatc aattacagca
     1681 ttatttgaaa tgaaaaaata aggcctgtaa agtgctggca gtatgaagag ccagcgaatc
     1741 gatgataatg aagaactgtc cccgaatttg aaaagttgag aactgtcccc gaaggagtga
     1801 cgcattatgg taagaaacag gaacaaaaca ggaaaacgaa gcggccagtc gctggtcgag
     1861 acggcgctgg ttttgccgct gctgttgctg atcctgatgg gtatcatcga ctttggcctt
     1921 ttgttcaaca gctacctcgt agtaagcaat gcatcacgtg acggcgcaag gcatgcggta
     1981 acgggagata ccgatgcgca gatcagggct gctgtctgca gtgccgcatc gacactggac
     2041 ctgacacgcc tggacatcac gatttccccg gatgaaggcg aaggcaggag cacgggagat
     2101 tcggtaactg tgacggttaa atttgagtac agcatggtca ctccgataat cggggcgttt
     2161 ttccccgata ctttcgatat agagtcaagt acgaccatgc ggtgtgaata atgccggagg
     2221 tgcttatgga gaagaaaaac aggaagatgc tgattattgc gctgactctg tctttgatta
     2281 cagccgccct gatctatatc tacatttccg gcataaaacc cgaacaggcc ccggaaaaag
     2341 aatacacaaa ggtatgtacc gcggcaagaa ccataactga gtttacacag attaccgccc
     2401 ctgatatcgc agaggtcgag gtggacaaag aactgtacag agaaggtatg gtaacagata
     2461 aagaacagat aatagggaaa tgggcattac aggacattgt aaaagacgag tttatcagga
     2521 aggaaaggat cggagacgaa gagagcctga atttttcata ttacatacca gaagggacaa
     2581 gagcagtcag catgaatgtc accgagcagg tcaatgcagc gaacctgctc agaccgggag
     2641 actatgtgga tgtgatcgca agcttcaata aggaagaaga agtatacggt gaacggaccg
     2701 tcacctatcc gaagattaca aaaatcatat tgcaaaatgt cagagtactt gctttaggcc
     2761 aggacagaaa actgcctgcc gaaaagctcc aggagcagcc agcgacaatt acgctggctg
     2821 taaaaaagga agatgtcgaa aaattcgtct atgctgccga atatggaatc atcaggctgg
     2881 cactcagacc tgcgggcgat aattcacgga ctgacagcca gggagccatc agggaggatg
     2941 tcacagggac gaaaggcgtg attgtgacag ataatgcgga cggttctgcc ggcgcttctg
     3001 agtgaaaact aacgaatcgg cctgattcag aacaatcagc tacattatga caggctgcaa
     3061 aactgttccg acggggccgg aaatacatgc ttcggaatgg attgccgtaa aatggccgat
     3121 aaacgccgca gagccgtttt tgactgcagt atcggaaaca tcgggtcggg aatcatcaga
     3181 cggaaagaat cggaatgtga aaaccgtcag gaggaaaatg atatggaaaa aatcaacgta
     3241 atggttgtag atgactcata tgaaacaagg agcaatataa aacagctttt gtcattcagc
     3301 aaaaatataa aggttgctgc ggaagccggc agcggagaag aagcgatcat gctggctaac
     3361 gaaatgaaac ctgacgtcat cctgatggat ataaacatgc cgggcataga cgggataaag
     3421 gctacagaag aaatttcgct gaatgttccc gattccgtag tgatcataat gtctgtgcag
     3481 ggcgagaccg aatatgtaag aaaagccatg gctgcaggcg cccgcgatta cctgtgcaag
     3541 ccgttcggcg gggacgagct ctccgagacg ataatcaaaa cttatgaaaa tgaaatgaga
     3601 cggcgggaaa aaattgctcc tgtgacggcg aaggaggaaa tcaggtccag agtcatcaca
     3661 gtattcagca caaagggcgg cgtcggaaaa acgacgatag cttccaacct ggccgtatct
     3721 attgcaagaa gcgaaaaaaa acgcgtggcg ctgttggact ttgaccttca gttcggcgat
     3781 gttgcgatta tgctgaacgt ttcggtcaag aacacaataa gcgacctggt gaaggagttc
     3841 ggtcaactgg acgaaagtat agtcgaagat tacctggtaa cgcatttttc aggcgtcagg
     3901 gtgctgcctg ctcctgcaaa gcccgaatat gccgaataca taacggcgaa tcatgttgag
     3961 aagataatca acattttaag ggattcataa aactatataa tcattgacac gtctgcgagt
     4021 ttccatgaaa cggttctgac atccctggac atgtcagaca aaatccttgt catatcgact
     4081 cttgaccttc cgacgataaa gaacgtcaaa tcagggctcg atgtgatgga aacgctcaat
     4141 tatccccagg ataagataca cgtggtcctg aacaaggcat ccgagcaatt cgggatcagg
     4201 tacagggact ttgaagacgt gctgcataag caaatctggt catatgtgcc cgaagacagc
     4261 cagacagtag ttacttcagc aaacaaaggc ttcccgttcg taatgaccag gactgaaaca
     4321 aaggttgcca aagcgatcat agacatggga aacgaactga tgatggaact ggagggcggc
     4381 gtaaacaaga aaccgccgtt aagaaagctg tttggttaac gggagggaaa tcaattgtct
     4441 ctgttggaaa gattgcagaa agaaagggca gttgaaactt cagaaagcgg gaaaagccaa
     4501 ggaacaagga atgtgcaggc tccgaaagag gatccgtttg aagatctgaa agtccgcata
     4561 catatgagga tcatcgaaga gataaactcc gggacagtca gagacgtcga ggctgaaggg
     4621 aaggaaagcg accttgaaca ggcgatttcc gggatagtgg aaagctatct tgacgaagag
     4681 ggcgtattta taacgaaact ggacaagcaa aagctgataa ccgagataat agatgaaacg
     4741 atcggattcg gcccgatcaa ccggcttata agggatccgt ccatatcgga gataatggtc
     4801 aacggccccc aaaatgtcta tgtcgaaaag aaaggcaggc tgatccgcac cgatgtggtg
     4861 ttcaaggatg atcagcatgt catgaaggtc atagagaaaa tcgttgcacc gctgggaaga
     4921 aggatagacg agagctcgcc catggtggat gccaggcttc cggacggttc gagagtgaac
     4981 gcgataatac cgccccttgc actgaacgga ccttcgctga cgatcagaaa gttctctgaa
     5041 aggccgttta cggtaaagga tctcataaac ttcggaacga tgacgcctga agtggcgatg
     5101 ttccttaaag cgtgcgtaga agcaaggctc aacatcgttg tatcgggcgg caccggaagc
     5161 ggaaaaacaa ctaccctgaa tgtgatttct tcattcatac cggatgatga aaggataata
     5221 acgattgaag atgctgcaga actgcagctt gcccaggagc atgtcgtcag gctcgagacg
     5281 cgtccgccca acatcgaggg caaaggagcc ataaccataa gggatctggt aaggaactcg
     5341 ctcaggatga ggccggacag gatagtagtc ggagaggtgc gaagcggcga agcgctggat
     5401 atgctgcagg ctatgaatac cggccatgac ggttcgctga caaccggaca cgccaattct
     5461 cctcgtgata tgctttcaag gctcgagacg atggtcctga tggcaggcat ggatctgccc
     5521 gtcagggcca tccgcgagca aatatcatct gcaatcgatc tgatcgtaca acaggccagg
     5581 ctgaaggacg gttctcgaaa aataacacat atcaccgagg tcaccggcat ggaaggcgat
     5641 gtcataacga tgcaggacat attcattttc aaacagcacg gcagggacga aaaaggcagg
     5701 ataatcggct caatagtacc gacaggcata aagcccaaat tcgtcgaaaa acttgcggac
     5761 gccggcatat cgcttccgcc ggacatattt gtcgccaagt aaatttttac gtggtcaggg
     5821 cagctaatcg ggaaggagta ccgtactaaa aatgcgaaga tcatgaacga acttaccgaa
     5881 ggaggagttc cattgatagg ccttataatc cttatgagtt tcatatcagc cgcggtcctc
     5941 tattatcagc tgctcatgtt attttcctcc ggaaaagact atatggccag gttaaggcgc
     6001 tacacagcgc cggaagggat gtatgaagaa aaaatgaaaa aaaggaggga cagttcttca
     6061 cttttgcgtg atgtcctttc aaaagggatc gggaagataa aattcctgga cggatacaag
     6121 aaaaggatcc aggcggatct tgcaagagcg cacctactgt tgaaagcaga agaattttta
     6181 gtatttgagg tcatcctttt ctcccttttc ttccttctga ccattttaat caaaggaaca
     6241 agatcatgga ttttggcaat aatcttcggg atcgccggtt ggattctgcc ttcgttcctg
     6301 cttaaaaaca ggatcaagaa gagaatgaaa ctgctgaacg aacagctcgg agatgcgatc
     6361 acaatgatgt ccaactcact gaaagcgggt tacagtttcc tgcagacggt agatatcgtg
     6421 gcagaagaaa tgaccggacc tgtttcggag gaatttgcaa tattcagcaa agaagtgagt
     6481 cttggactca atactgaaaa agcgctggaa aacctgtctg caagggtcag aagcgaggac
     6541 cttgaccttg cggtgaccgc tgtgatgata cagcggcagg taggaggcaa cctggccgaa
     6601 gtgcttgaca aaataacgga gacgatcagg gacagagtca ggatcaaagg cgaactcaaa
     6661 gcagtgacgg cgcaggggag aacgtcggga ctcgttattt cgctgctccc tgtcgtcctg
     6721 tgtctaatca tttatatgat aaatcctgct caaatgagcc tgctgtttac acggccgctg
     6781 ggaataataa tggttgcggt tgctgcagtc atggaactga caggcataat gctgatccga
     6841 aggatcgtaa gaatagagat ttaaggaggc gggacatgtg ccggttttga ttattctgct
     6901 ggctttcgca agttgttttt tgcttttcta tactgttttg tcgatcgccg gcagtgatga
     6961 attgataata tcctccaggc taaagagtat aaaagactcc gggacgaaaa aggaagacgg
     7021 cgaactggac cagccgctgc tgaacagggt aatcaggcca atgctcgatc atttgggcaa
     7081 agtcatgatg cggataactc cgaaggaaat gatctcatcc ctggaataca agattaccag
     7141 ggccggaacc ccatggaacc tctcggtcag ggactgggtc aacatacagg cgtttctggt
     7201 tgccggaatc ccgctggtca cttttattgc attgaaaaag tccggcgtac ctggaggtac
     7261 gatactttta ttcctgatat ccgaagcggc ccttggattt gctctgccgg ggtttatcct
     7321 ggggaaaatg ctcacggcga ggcaaaaagc ggtattgaat tcactgccgg atgtgatcga
     7381 ccttattact gtaatcgtcg aagccggatt ggggtttgac ggcgcgctga tgaaagtcgt
     7441 ggaaaaaaag ccgggacctc tggctgaaga gtttgacaag gtcctgcagg aaataaaggt
     7501 cggaaggaat aagaaagacg cattgcggga catggcgcaa agactggata tacatgattt
     7561 taccgctttc gtcggcgcca ttatccaggc agaacagttc ggagtgggta ttgctcaggt
     7621 gcttaggatc cagtcggagc aaatcaggct caagaggaaa cagcgtgccc aggagcgcgc
     7681 aatgaaggtg cctgtcagga tgttgatccc aatggtcatg ttcatattcc cgacgctgtt
     7741 cgtcgtgctt ctcgggcctg tagtgataag gcttattgaa cagttttccg gcatgtagac
     7801 gcagttattc acagttctcc ggcatgcagg cattatactg atggcatatg gaattccgga
     7861 tggtcgtaaa gattcataga aattggttta atatagttga agatagttgt agatgatagt
     7921 ataatgtgtc ttacataatc aggtgatcgt tcataagtga ctaatggatt ttcggaggct
     7981 gtgaaatggg ttttttggat gatataagaa acaagatcct tatacatgac ggctcaaagg
     8041 gatacatgct gcagagaatg gggctgaaag gcggggagtg cggcgaatac tggaacctga
     8101 ccaaccagga tgcagtacgt gcaatctaca gggcatatct cgaagccggg tcggatgtgc
     8161 tgcagaccaa cacttttacc ggaaacagga tccatcttgg ccgattcggc ctcggggaca
     8221 gaacatatga aatcaattac tggggaacca ggttagcaaa agaagttgca ggcgacaggg
     8281 cctatgtgag cgcttctgta ggtcctaccg gctcacttat ggaaccgttg ggaaatctct
     8341 ccttcgaaag tgcctgcgaa gtttaccgtg aacaggtgaa agcgctggcc gacggcggtg
     8401 cggatgttat caatttcgag acgtttacgg acctgtcaga gctgcgggct gcgtacctgg
     8461 ctgccagaga tgtgacggat ctgccagtga tctgctcact ggctttcgaa accaatggac
     8521 ggacgctgat gggcaccgat ccgtttatag cggtcaacgt gcttaagtcc atgggagccg
     8581 atatggttgg ggcaaactgt tcttttggcc ccgagcatat gaaaggcatt atagagggta
     8641 tgtacaaggc aggcggcggg tatctgagcg ttaagccaaa cgcaggtctg ccgtcggtat
     8701 cgggcaatgc agttaagtat catgaaacac ccgagcattt cgccgacctg tcggcagaat
     8761 ttgtaaaatt cggggcaagg ctcataggcg gctgctgcgg aacgacgccg gacttcataa
     8821 aagcgctcaa aaataaactg tcggagacgc aacccgtgac cgttgccgga aggacagcta
     8881 tatgcattac atccgacgta aaatacatta atgtgaaaga ccttgacaag gccaatatat
     8941 acaggatcga tgccgcaaaa gatcaaacgc tgtatgaagc gttgaaaaac aatgactttt
     9001 catgggcgga agaaacagcg ctcgatctgg cagccgaagg ctatgatgca atcctggtaa
     9061 acacggacag tgcgggcgga gacgccgggc tgctggcgaa tgtgattgac cggctgcaat
     9121 ggtatgtgaa ggatcctttc attattgaaa ccaacaatgc agcggctctt gagagagcgc
     9181 tgaggatcta cagggggact gccggtgtcg ttattaacag cagttcagat gatgaattaa
     9241 ggaatattgc agaaaagtac ggaagtgtga ttatttcccg agatatatag cataaatgcc
     9301 aatccatgtt tgtccagact gttcggctgt gcgaacctgg ttgactggcg agcatattga
     9361 agttgatacc tgtacatgcg gatgctaaaa ttaaataaag gaaatcgcgt tttcgtgccc
     9421 gaaatgtttc gcacccgggt gggtgcgaaa taggcccgag accgatagat ttgcatctga
     9481 aagcgctcaa aagcatgggc gcgaaaataa acgacgcgca tcgcggtttc atccactgtg
     9541 aggcagaaag actgaaggga tgcgacatac agcttgatta ccccagtgta ggcgcaacgg
     9601 aaaacatcat gcttgcatcg gtctttgccg acggggagac gatcatcagg aatgcagcaa
     9661 aggagcctga gatagttgac ctgcagaact acctgcaggc aactggcgta cgggtgacag
     9721 gtgcagggac gagcatcatc aggatcgccg gaggtgaaag caagctaagg aatgcggagc
     9781 atacgataat caccgaccgg atagtggcag gtacatatat ggcggcagca ggtataacag
     9841 gcgggaacct gcttttgaaa aacgtcatcc ccgaaaacct cagttcagtt atttctgtcc
     9901 tcagggaaag cggatgcagg atccgcgtca aagacggtat ggtacacgtt gccggtcctg
     9961 ccaggctgcg tgcagtagat atcgtaagaa cgcttccata cccgggtttt ccgacagata
    10021 tgcaggcaca gctggtggcg atgctgacga tagccagagg taccagcata gttgttgaaa
    10081 ctgtttttga aaacaggtac aaacatgttg acgaactgct gaggatgggc gccaatatca
    10141 agctggaggg gcgtatagcg gtcataaagg gagtcaggaa gctggtcgga gcaaatgtat
    10201 gggcaagaga cctcagaggc ggagcagcac tggtcctggc cgggcttgcg gccgaggggg
    10261 agacagtggt atcggatatc cggcatattg acaggggata cgaaagaatc gaagagaaat
    10321 tgcggcaagt cggagcgatg atcaaaagag tagactgaga cagacagtac aacttgataa
    10381 aatcctctca ggaccactaa cgtcaggagg ataaatatga agctgcgcag gataaaaccc
    10441 atgcaggaac ctgcatacgt gataaacagg agaaaaagaa gaaagcagag aatatggcgg
    10501 gcagtaaaat cgatccttgt taccgcatta tttgttttta cagtaatata tgcttctctt
    10561 tctccttttt ttaatataaa ggatattcga gtggccggtt cgaccaggta cagcactgcg
    10621 gaactgtcaa ctgcttcggg catcgtgacg ggccgcaacg gattcagggt gctgttttcc
    10681 agcaaatcaa agcccggcct tttccgcata ggcgacgccg agcaggccat aatcgacagt
    10741 tgcccgtatg ttaagagcgc gaaagtcagg tatattatcc cgggaacggt caggatcgat
    10801 ataacggagc gtgaacctgc cgccataatc gatattaaag gaatccagct tctgatagat
    10861 aaagaaggat accttctgga aatagatccc gtcttcgatg cggaaacgga tgaaagcgga
    10921 cttcctgtga taaggaccgc tgaaccaggt tcggcaaatc cgggtaaaaa attggatatt
    10981 cctgaagaaa tgctcttatc agcattcaaa gtatttgata caataaaaga gatggatgcc
    11041 gtgaatgatg ataaattgct gccgatggtt gattacgttg atacaagcga cctttcgaac
    11101 attcggcttt cactgcaggc tcgcatcgtc gtcaatcttg gcaaggctga tgagcttcat
    11161 tacaaaatca acgcagcagc aagcatattc accaggaaca tcaaaaaaac tgaaaggggt
    11221 acgctggact tctccgttgg gaaagatccg gtatttactc ctgagagcgg aggttaggtg
    11281 cttatgttgc caatattggg aatgatcatc ggaatactga tcggtgtttt cctgatacct
    11341 gtaaacatac cccagcagta ttccatttat cttgctgtgg ccatccttgc cgcacttgac
    11401 tccgtattcg gcggagtcgc atcgtctttg cagggtaaat tcgatatgaa gatctttctt
    11461 tcgggatttt tcggcaatgc gatccttgcg gcgatattgg cgtatatagg cgatcagatg
    11521 gggctgcagc tgtaccttgc ggcaattttc gcatttggca acagaatttt cttgaatttt
    11581 gcagttattc gcagatatat gttgaacaag tcggataaaa aagataatat atagtttgta
    11641 gggtagttta cagggggtca tttccgtgag tgattggatc gtaagtattg acatcgggac
    11701 gtcgaatgtt tgtgcactta tcggaaaagt caacaaatat tcacaaatag agatcgcagg
    11761 cagaggtatg gcgccttgca gcgggcttaa aaaaggcgtc atcgttgata tagacagtac
    11821 cgctgcaagt atcaggaaag ctgtcgaaga agccgaaaca gatgcaaaca tgcgtgtcga
    11881 ttcagcttat gtcaacatac acggcatgca tgtcaatatc gtaaataata gaagctggat
    11941 gagcataaac ggaagggaaa taaccaggga cgatctggat gatatactcc gggatgtcag
    12001 ggatatcgat atcccggaag acagacagct tatagatgtt atcccgaggc agttcataat
    12061 cgacggatac gatgagataa ccgatccggt gggaatggta ggcaccaggc ttgaagtcga
    12121 tgcggacatg cttgtcggga aaatcacatc ggcccagaat attataaaaa gtatggaacg
    12181 ggcagatgta aagctggatg gaatcgtcgc ggaagcgttt gcttcggcag agatatgtct
    12241 gacgccggat gaacgagata taggagctgt gctgctggac gtcgggggaa gcataaccga
    12301 cgtttcggta tttcagggca aacggcttgt tttttatgac tcgatacctg tcggcggcga
    12361 ccatataacg aatgacattg ccataggtct caggatatcg aatacggaag ccgaaaagat
    12421 aaagcgtcag tttgagcttg cgctgacttc gctgataaag aatgaccagg aaatatccgt
    12481 acttgatata aatgaaaaca ggaaaaagaa cgtgaaagtt tcggaagttg tagaaatcat
    12541 cgaagcccgc gtgtatgaga tattctcgtt atgcaaaggc ctgctggaca aggccggaat
    12601 aaacattcca agggagggcg gcgtcattat gaccggcgcc gggatatctt atgtcgatgg
    12661 ctgcaagcag ctggcgcagg aagtgttcga tcttccggta agagttgtgc aggcaggaca
    12721 gatacccggg gttttgaaac cggaattcat agcatctgca ggcatgatca ggtatgtcgc
    12781 cagtgtcagg aagggcggca gcagggcgag cgagatcagg ctcaggaaac aggagaagca
    12841 gaaaaaggat gaatcggttt ttgaaaagat attgaatttt atcaaaaaaa tgttttaatt
    12901 catcaatgct cctttgtttg aactttttgc taaactcatc ttgctttaat gaatggaata
    12961 ggatattatt tatttgtgcc aaaccaatga caaaaggggg attcgatttt gctggagttt
    13021 gatattgata tggaacagtt tgcccagata aaagtcatcg gtgtcggcgg cggaggcaac
    13081 aatgccgtaa acaggatgat aactgccggt ctcagaggtg ttgagttcat agcgataaat
    13141 acagataagc aggccctttt tctttccaag gcaaatacaa aaatccagat cggagacaaa
    13201 ctgacaaaag gactcggagc aggcgcaaat cctgaaatcg gagagaaagc tgctaatgaa
    13261 agcaaagatg agatcgccca ggccattaag ggggccgaca tggtcttcgt aacagcaggt
    13321 atgggcggcg gaaccggtac cggcgctgca cctatagtag ctcagatagc aaaagagatg
    13381 gggatactca cagtaggtgt ggtaaccaaa ccttttatgt ttgaaggccg caagagaatg
    13441 cagtatgcgg aacagggcat agagaacctc aagggtgttg tggataccct tgttacgata
    13501 cctaacgaca gattgcttca ggttgcagaa aagaaaacgt cgattatcga ggcgttcaaa
    13561 atggccgacg acgtactgcg tcagggtgta cagggaatat cggatcttat agcggtacct
    13621 ggtttgataa acctcgattt tgcagacgtt aagacgataa tgtacaatac cgggcttgcg
    13681 cacatgggaa tcggcagagc ttcaggcgac ggcagagccg aagaagctgc aaaacaggca
    13741 atccagagcc ccctgcttga aacgacgatc gacggtgccc gcggcgttat tatcaatatc
    13801 accggcggac cggatcttgg actcttcgaa gtcaatatgg ctgcagaact tgttcagaaa
    13861 tctgctgacc ctgatgccaa cattatcgtt ggtgcggtca tcgatgagaa cctcaaggat
    13921 gaaatactca ttaccgttat agccaccggc tttgacaaga gcccgtcaat gaagaaaata
    13981 gacaagatag cggaaaaatc tccggcaaag caggaacaga ctgttccaag acgtcagacg
    14041 gccagcgata tatccgaaga tgagcttgag ataccaactt tcctgaggaa aaacagattc
    14101 agataaacct gacaacgaac aaaaaccctg ggcaatctgc ccggggtttt ttgcgcttgc
    14161 cgggacacag cgccagccag gggacagttc ttcgaaatgg gggacagttc ttcgaatgag
    14221 ggacagttct tcagaatttg ctgacagatg cggcctttca aattcgtgtt ttttactgat
    14281 gaagaattct ttccgtgaaa accatgtttg caagatgaat gttcagcagc taacggatca
    14341 tataaagtaa ctgctctgat gaattttgga taaatgctct tagtgaatat tcaaaggacc
    14401 atcccttagc attaactcgc agaactgtct ccattgttaa cttctgcaga actgtccctc
    14461 ttcctttaac ttctgcagaa ctgtccctct tcctttatct cctgaaactt cccttgaaaa
    14521 aaccttattt ctacaggtgt ttgtgacata aaaatcatgt ttgatcaaat ataatttaag
    14581 tatgttgtta tacataaatg cccggacata tgaatatatg taaaataggg gatgtctggc
    14641 atttcagctt gtgaggtaat actgtggaga tttatctgga tatagtcatc ctggagaata
    14701 tagtcatcaa ttacttgata cttttagtta catccagatt ctcaaagaac agaacgagca
    14761 gcctcaggct ttttctcggc tctgtagcag gcgctgctta tcttgtgctt atgatactgc
    14821 tgccggatac gaaagtgtat gccacactgc tgtcaaaatt tctgctgtcc gtcgggatgg
    14881 ttgccataac gttcagtttc agaaagcttt cggttttcct gaaaacgctg gcgctgtttt
    14941 atgcaacgac gttcattttc gcaggagccg cttttgcgct tatgttcttc agcaaagact
    15001 gggggatcat aagaaacggc gttcttatca caccgctgtc ttttattgat gcaaaatggt
    15061 cagaactgct gctggcagtc gcagtcgcat tgattatttt ccgtgtgatc tgggatgcat
    15121 tgcaaagcaa atttatcaaa gaaaaactgc tggtcaatat aagcatcgca atggggaaca
    15181 agtccatcga gttgcccgcg ctggtggaca caggcaactc cctgcacgat ccgcttacaa
    15241 aactgcctgt agttgtcgtg gaattttccg cgataaagga cctgcttcct gatgatatca
    15301 aaagcatttt tgagcaaaac aatgaaaacg atctgaattc ggtcacttca acgatctcgt
    15361 cttccaactg gttcaccagg ttcaggctga tcccgtttaa gtcgcttggc agggaaaacg
    15421 gcatgatgat cgggttcagg ccggattaca tagagatagg tgctgacgaa ggcaagaaag
    15481 atatccggga tgttatcgtc ggcatttaca acagaacgct gtcggcaaat gagcagtaca
    15541 gagctctgat gaatcctgag cttatataga tatagcaacg aaaggagatt gcagatgaaa
    15601 gtattcaaca aagtcaggct gatcttaaga attcattact gggagttgct tgcgaggatc
    15661 agaagaagga tcgggcttcc aatatattat ataggaggca gcgaggcatt gccgccgccc
    15721 cttacagtcg acgaggaaac gtatctgctc gcaaggctgg aagaacagga ttacggagta
    15781 aaaagcgtgc tgatagagcg caatctcagg cttgtcgtat acatagcccg aaagttcgag
    15841 aataccggcg ttggagtcga ggatcttgtt tccatcggaa cgattggcct tattaaagca
    15901 ataaatactt ttaacccgtc aaagaatata aaactggcca cttacgcatc gagatgcatt
    15961 gaaaatgaaa tactgatgta cctgaggaga aacaacaggg tcaaaacgga aatctcgatc
    16021 gatgaacccc tcaatgtgga ctgggacgga aatgaacttc tgctttcgga tatactggga
    16081 actgaaagcg atatcattta caggagcctc gaagatgaga tcgacagaga actgttgaat
    16141 acggccatga aaaaactgtc gagccgtgaa aagaaaatca tggaattgag gttcgggctg
    16201 acgaacggag ttgaaaaaac acaaaaggaa gttgccgaca tgcttggtat ttcccagtcc
    16261 tatatctctc gtctggagaa gaggataata agtcgtctga agaaagagat caacaggatg
    16321 gtgtaaccac caaacccgct gttgtcaagg cctttagcga atattttgtg aagaagtttc
    16381 agtataaaaa agacctcccg ggcaataatg ttactgtaca aaaagtgccg ggaggttttt
    16441 acatgatggt caacaaagtt gaaatctgcg gagttaacac ttcaaaactg ccggttttat
    16501 ccaatgagca aaagaaagct ctctttgaaa gaatgcacaa gggcgatgct tctgcccgtg
    16561 aggaattcat caacgggaat ctcaggctcg ttttgagcgt aatccagaga ttcaacaaca
    16621 gaggcgagta tgtggatgat ttgttccagg tgggctgcat aggcctcata aaagcaattg
    16681 acaattttga cgtatcccag aatgtgaaat tctctaccta tgcagttccg atgataatag
    16741 gcgaaatccg gagatacctg agagataaca attcgatcag ggtcagcaga tctttaaggg
    16801 acatcgcgta taaagcactt caggcaaaag aaaggctgac gaacaagaat tccaaggaac
    16861 ctacgatttc tgaaatcgct gaagagttga agcttcccaa agaagaggtc gtttttgcgc
    16921 tcgatgccat ccaggatcct atttcgctat ttgagtctgt gtaccatgat ggcggtgacg
    16981 ccatatatgt aatggatcag gtcaaagatg aaaaaaatat cgatgaaaac tggcttgaag
    17041 gcatttcgct gaaggaagcg atgaggaaac tgaacgacag ggaaaaactg atactgaacc
    17101 ttcgtttctt cgagggcagg acacagatgg aggtagcgga ggaaatcgga atatcacagg
    17161 cccaggtatc aaggctggag aaaactgcgc ttatgcatat gagaaaacat atatgactaa
    17221 gcggaacatg aactgcgaaa tactcatgtg aatgaggaat atgagtatca gaatgcagat
    17281 atgaagataa aacgacatga caacataacg gaaagcataa atgtgcaaac agtgcttacg
    17341 catatagggc acagcaaaga gaaaaacgga aaagtgcctt cggaatggag gactgtcccc
    17401 gaggtcagag aactcttccc gggatggaga accggccctg gaatggaaat tggaacttct
    17461 ccttgatcag aaaattagaa ctgtctccgg gtgtccctgg gtaaaatcga gaactgtccc
    17521 ctgccaatgt cccattatga taattgagcg gcagcggaca tataatttat atgaaaccgt
    17581 acttcagtcc atttagttga cggaacgata gtaaagattg gtgggggtcc tatgaatcgt
    17641 tcttcagact tcaggca
//
LOCUS       DAOSCC010000016        17438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2254_length_17438_cov_4.778864, whole genome shotgun sequence.
ACCESSION   DAOSCC010000016 DAOSCC010000000
VERSION     DAOSCC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_17438_cov_4.778864"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3638
                     /locus_tag="PLG72_01850"
     CDS             <1..3638
                     /locus_tag="PLG72_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79578.1"
                     /translation="GSNQCNADFNIEEYIVQNEPPFLMEPKCVIPQTGFDIVEFVAYD
                     GTDMNGIAERKVLINGVEVDDEFFFSGKFIFGDGQDGLKKIDVYYTDYDGNTAFHSTW
                     AYIYNTRPTAQFRFSGTFKQNRKLTVTDISNLAGSDIVNSAYPIDSWSWKIRAVSGDQ
                     SSIRKKDISKTQKDLLFKKPGSYEVELTVTNTLGRVSEPYIIRFEIFPDYEPALEIDL
                     DNSVISRTETVSAWNYNACSTDNDIISKNIIELWYDSDNDGEYDKLLETYDGRNGFPE
                     FRPVGLGRYKFVNTVEESFGEDTIEEFITESDRVTRTLEREILVDNIQPMAGLYISMP
                     LNRPQIDTFIMLDSGLDEEDVRYIKDNRIEINNSLRSANILSNVEVWDLRTYRYEMPA
                     SKSVHTGSSLPPETVEYSSNGYYGVLKRTSYINNRYQTDEGYYVTKVDKKTVTGTLSG
                     WSEAQFVYTAAGWRQISSYSTDQPTMTYNQDGYYGVLSKVSFTVDTDNGRPSGSGKIG
                     DTYTWRKTYTGYYSGEVTKTISIWIPNLVWHNDYTGFYSGTVSKEVKQPYTDPFRPTS
                     QKYIVYIAGDSLHDMNDLSFALNKSGARLILIGHESIRRQVGHDHFIEKGRPLSELMS
                     EALDIIADTGVAEKYTLLAGLDDLHLSVADFDEENDPIAERKFLYVHDPNYFDNGSGL
                     EQFCAEHYSDSDGWVDIFLTKLNKPGKYTIYRRVKDDPSEDPAFDSFDRYSGTPFVEV
                     YAHRKPVAGATLDWDYDPEENVYRTTWVDNSYDPDHQYSRMDKGIVERKIMYREAGGM
                     WNYTIPDKLKPGTYELYYYVRDPENTWSDPFILNFRLEEAPPIQFDASLRALDNAFSL
                     SDIPASEKLEAYDIRTRYPGNLKLEMALFKGALRESQVKTVVFGNDTGTRVNNDIYWN
                     NVIYTIPADLPDGIYDFRISAVADDGKSAAKSFPVTVSTPLNLVPHMPAELSGGARAH
                     VSAETSKYAGAVSITLFHGTEHARTFSLTAPAGAEGAVKHWESVVSVPYGIPDGEYTA
                     RFTATAPNGKSQSKDVRFGLTNIAITDVKLSGYWNHWRGQTDIFGKRMTVEPHRFLSL
                     ECVKVDIRTKGDPEKVVIRFSPELEAMRYTDPNGHTYDYGKDFFGYQVRFPEDSTLTV
                     TDNQAQWEYYLPLAPSTKDWDNRRLRPQYRMTVTVYKGESFAEYVIDDIDITGNVYDL
                     TYIQPKR"
     gene            3746..4729
                     /locus_tag="PLG72_01855"
     CDS             3746..4729
                     /locus_tag="PLG72_01855"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ79579.1"
                     /translation="MSILRNAKIFAGSSGRVFAEKMCEFIGGRLSESEVIRFSEGNTY
                     VKASETVRGMDVYLVQPIGLRPNDEFVEILFWADAFKRASANTVTVIMPYFGYAKADK
                     KDEPRVSIRARVCAECMELAGVDRIVTMDLHSPQVQGFFKKPLDHLRALPVLCEYIRA
                     TVNDTLVVVSPDAGFAKQARKYADYLGASIAIGDKTRRAHDEKAQILDLIGDVAGKTA
                     LIVDDFTLSAGTLADLSHLLKERGAVRIIACVTHLLLSEKSVERINGSPIDLVIGTDS
                     VENRNLLKSDKVRLVSVAPLFAQALIRIHNNESVSPLFTNISENVKYQSYR"
     gene            4822..5589
                     /locus_tag="PLG72_01860"
     CDS             4822..5589
                     /locus_tag="PLG72_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOJ79580.1"
                     /translation="MWTRAELKYRAREALRGNYWKAFLVSLVIMIATEGLGGGSGRAG
                     HDEISSSGGSFFGSIGSIEWAAVIAAILGFFATIFVISLALQIFVMNPLEVGGRRYFI
                     RTAQYMDNSRCFRFAFNGHNYLGIVGGMALKDLYLFLWTLLLIIPGIVKSYAYRMVPY
                     ILADNPNIGAGRAIELSERMTYGHKFDIFILDLSFLGWYLLGLLCLLVGGLFVKPYEY
                     ATNAELYLTLRRNAIESGYCTPWELNLYDPVHDEYDL"
     gene            5637..6503
                     /locus_tag="PLG72_01865"
     CDS             5637..6503
                     /locus_tag="PLG72_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79581.1"
                     /translation="MWLDKLEKKIGRYCIRNLMLYITTLNGVVFVLTYLAPELDFVNK
                     LTLVPSLVMKGEVWRLVTFLFIPMNFNPVWVIFSLYFYYMIGSSLEHEWGSFKFNVYY
                     LVGVLGTIAGAFLGGLGTSTFLNLSLIFAFAYLYPNYQLVIFFFFPVKIKYLAIFYAG
                     GLVLTFMLSPIIGLITVLGSAFNFILFFWKDIYYRLKFNRKAYMSQKEFKAKIPKIHV
                     MHRCTICGKTDVEDRNMDFRYCVECEGDYEYCMDHLYNHEHVKANASAGNDDSTGNDG
                     NMGNNGGNARAN"
     gene            complement(6553..7698)
                     /gene="wecB"
                     /locus_tag="PLG72_01870"
     CDS             complement(6553..7698)
                     /gene="wecB"
                     /locus_tag="PLG72_01870"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ79582.1"
                     /translation="MRKLRIMPIFGTRPDAVKMAPLVKELGKCPEIEPVVCVTAQHRQ
                     MLDQVLDIFRIKPDIDLDIMLDRQTLEQVAARTLDRLSVVLERERPDMVLVHGDTSTC
                     FISSLAAFYKKIPIGHVEAGLRTFDKYSPFPEEMNRKLTAALADLHFAPTPSNKSNLL
                     SEGINPDKIYITGNTVIDAMQTTVREDYVFNSDEMRKIDIRGRRVIAVTAHRRENLGS
                     PLEQICLALNDIASEYDDVCVIYAVHLNPAVRETAFRILGSNPKVHLIDPLDVQDMHN
                     LMARSYIVMTDSGGLQEEAPALGKPVLVMRKETERPEAVEAGTVKLAGIERERIKALA
                     SQLLDDPAEYEKMAKAVNPYGDGKASQRIVKAILYEFGITNEKPAEF"
     gene            complement(7695..8855)
                     /locus_tag="PLG72_01875"
     CDS             complement(7695..8855)
                     /locus_tag="PLG72_01875"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ79583.1"
                     /translation="MSYITEYVITFTLAFIISFSATSIVRRLAFKVGGVDVPKDDRRM
                     HKKPIALMGGLAIICGFIISLVFDLATASNIITPGKELLGLVSGIAIIMVMGILDDTR
                     TLKPWAKLSFQLVAAICTVAISGTRISLVTNPFGDDKYIELSPYISIPLTIIWIVGIT
                     NAINLIDGLDGLAAGVSSISSLSLFFVTLLRADLDPAKAVYIALITAALSGSTLGFLP
                     FNFNPAKIFLGETGAAFLGFTLGVISIQGVMKSYAAISIVIPLLVLGLPLFDTAFAII
                     RRLFRHKSIMQADRGHLHHRLIDMGLSQRQSVLILYTASASLGLCAIVLADRGALSAI
                     VMLLAAAIFVAGGAKYINDRGLVDDRKTTDDGPSKPAVPDTDIKKQSDGVTK"
     gene            complement(8871..9308)
                     /locus_tag="PLG72_01880"
     CDS             complement(8871..9308)
                     /locus_tag="PLG72_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOJ79584.1"
                     /translation="MRPSWDEYFMGIVELVKERSTCLRRQVGALIVKDKRILATGYNG
                     APSGCKHCSEVGCLREQMGIPSGQRHELCRAIHAEQNALVQAAYSGTSVNGGTLYVTH
                     QPCVLCAKMAINAGIKKIVFKGDYPDDLAMELLKEAGVRVVRV"
     gene            complement(9444..10073)
                     /gene="upp"
                     /locus_tag="PLG72_01885"
     CDS             complement(9444..10073)
                     /gene="upp"
                     /locus_tag="PLG72_01885"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOJ79585.1"
                     /translation="MEKVFVFDHPLIQHKISLLRDKNTNVKEFRELVSEIAMLMGYEV
                     TRNMPLKEVEIETPVGIAKTKVISGKKLGIVPILRAGLGMVDGMLNLLPMAKVGHIGL
                     YRDPETLEPVEYYCKLPVDAHEREIVVLDPMLATGGSASAAIRFIKEKGVSNIKLMCL
                     IAAKAGIERINKDHPDVEIYCAAVDEKLNDHGYIVPGLGDAGDRLFGTK"
     gene            complement(10089..10547)
                     /gene="rpiB"
                     /locus_tag="PLG72_01890"
     CDS             complement(10089..10547)
                     /gene="rpiB"
                     /locus_tag="PLG72_01890"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ79586.1"
                     /translation="MLAIASDHAGFSLKSEIIKHLEEINVPFEDFGTCDCTTSVDYPD
                     YGLKVAEAVASGTHERGIIICGTGIGISIAANKVPGIRAALCTDTYMARMSREHNDAN
                     VLALGARVLGVGLAIDIVDAWLNASFLGGRHKQRVDKFADIESKYSKTHT"
     gene            complement(10618..11067)
                     /locus_tag="PLG72_01895"
     CDS             complement(10618..11067)
                     /locus_tag="PLG72_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOJ79587.1"
                     /translation="MKKILFVCTGNTCRSPMAAALFNRLAATAGIGYTAASAGLAAAE
                     GSPASINAIKAMEGYPGADLSYHASKRLSHADIEEACLILTMERIHRQYILSMYPEAY
                     QKVFTLKEYVCGTDGDITDPYGGDESVYKHCAEILSQAVEKLIEKLK"
     gene            complement(11078..12145)
                     /locus_tag="PLG72_01900"
     CDS             complement(11078..12145)
                     /locus_tag="PLG72_01900"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ79588.1"
                     /translation="MKTQIIKIDTSTSNWDKQLDQAAEVLRSGGLVAFPTETVYGLGA
                     NALDEEAVKSIYRAKGRPSDNPLIVHIADTAAVKDLTDSIPGTAQALMDAFWPGPLTL
                     VMPRSSLVPDIVTAGLDTVAVRMPSHPIASALIKKAGVPVAAPSANSSGRPSPTLARH
                     VIEDMMGKIDVIIDGGNAEVGVESTVLDITVDPPVILRPGGVTLEQLRHVLGNVISDQ
                     TPGISDMAGTPKSPGMKYRHYSPKATMLLIQGDPEKVAAEISKRAGLYHKEGTAVGIL
                     VTDETAGLYESSLYSYCKILSLGSRKEPKTLAANLFRCLREFDEKGVEVIMAETTDVR
                     GIGQAVMNRLMKAAGGNIVKL"
     gene            complement(12230..12970)
                     /locus_tag="PLG72_01905"
     CDS             complement(12230..12970)
                     /locus_tag="PLG72_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ79589.1"
                     /translation="MGHLLKITFLGLFSGLAGTGIGGLSAFLISKTSNRIMSTILEFS
                     AGLMTAVVCFELLPEAFSLGGTVFTFLGIITGILTVVAIESFLKKSRSLGKGQNTGLL
                     RAGILMAIAIAMHNFPEGFAVGSGFEASTSLGVTITAVIIIHDIPEGIAMAVPMRAGG
                     FSKFKAFAYTVLSGVPMGLGALLGAVLGSVSDSLTAACLGFAGGAMLYIIYGELVPES
                     KRLYLGRLSSLANVLGILCGIIISVYNT"
     gene            complement(13177..14256)
                     /gene="prfA"
                     /locus_tag="PLG72_01910"
     CDS             complement(13177..14256)
                     /gene="prfA"
                     /locus_tag="PLG72_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ79590.1"
                     /translation="MFDKLDAAENRYEEISMKLSDPQVIADQDEYRKLMKEFSGLEEI
                     VSKYREYKKTKKDIEETRELLEENSDKEFRELVQSELDEAKTKLGTIENELKQLLIPK
                     DPNDNRNVIVEIRGGAGGEEAALFAGDLFRMYTRYCERHQWKSEILDIHETEIGGIKE
                     VIFSIEGKGAYSRLKYESGVHRVQRVPVTEAGGRIHTSTVTVAVLPEVDEVDVQIDPN
                     DLQIDTYRASGAGGQHVNKTESAIRITHIPTGIVVTCQDERSQHKNRDKAMRILRSKL
                     YEMAQQQQESEIAQTRKSQVGTGERSERIRTYNFPQGRVSDHRIGLTLYKIGEIMDGD
                     LDELIDALITADQSQKLGSAAGGED"
     gene            complement(14578..15798)
                     /locus_tag="PLG72_01915"
     CDS             complement(14578..15798)
                     /locus_tag="PLG72_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOJ79591.1"
                     /translation="MSISEEKMMILKMLQEGRINSEEAAKLLEALEAKKEPSSYGTGP
                     RGSHGPKPPQRNYYDEVAKVRERIDEWRRELSKNYNQNDFDKMVEEFSAKAEKFGKNV
                     ATAAFGIADKVADFIGSIIDTSAFNIFGSCVTEEQQYEVAAAEGMNLELQATNGQVTV
                     KKHQEDKILITAKIRTPQENAKNAIAFSNENGTVSVKLAKPDTYNLSVSYDVLLPAVK
                     FNQVALETKNGKIYVEDVTSEEFVSVTKNAVIDLTGVNSGRIAAETKNAKVSANYIIG
                     RDVNFGTSNATIEIKNLKAVKLTAVTSNGKILVDNVQNYDNENVAELNLRTSNANIKA
                     NMNDSENKGYKISARTSNGDINLLIPNLLYRNAMRTDRFNRQADAETENYAAAAQKVN
                     IQAETSNGYIEVIK"
     gene            complement(15836..16204)
                     /locus_tag="PLG72_01920"
     CDS             complement(15836..16204)
                     /locus_tag="PLG72_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOJ79592.1"
                     /translation="MKKEALGRCPVCGQDTEVTAIYCSNCDTKIEGHFTLCRFCRLTA
                     EQKSFIETFIKCRGNIKEVERELGISYPTVKSRLEDAANALGYGMQSYENPESQKRME
                     ILDMLDNGLISVEEALELLK"
     gene            16450..17199
                     /locus_tag="PLG72_01925"
     CDS             16450..17199
                     /locus_tag="PLG72_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79593.1"
                     /translation="MQPGNANSGEVIPVIQTRANGRSVTELQVDFVVDGEIEQVPMFV
                     VGDLRTQDTLDEVRIYFHCSNVPGLQAYRKPMFKSAHLEMGDPGLLTGAVREYYEALH
                     RAPSLDVDRIIGCMSENCIFGGYEPYNSDHKPAATLAEIREKYEYMSRYIPRCVGMRY
                     ETIIDNGRTCVIEWVHIVSRAGQEEMARVAMSGIAAYERGDDGLLCSIRICDYAGLER
                     TIDWTKTPLTKEEAQRINFVEEFPQGVGLKK"
BASE COUNT         4182 a         4201 c         4372 g         4683 t
ORIGIN      
        1 agggaagcaa ccagtgcaat gcagatttca atatagaaga gtacattgtt caaaatgaac
       61 cgccctttct gatggaacca aagtgtgtca taccgcagac aggcttcgac atagtggagt
      121 tcgttgctta tgacggcacg gacatgaatg ggattgcaga acgcaaagta ctcataaatg
      181 gcgtcgaagt ggatgatgaa ttctttttca gcgggaaatt catttttggc gacggccagg
      241 acggtcttaa gaaaattgac gtatattaca ctgattatga tgggaatacc gcctttcaca
      301 gtacatgggc ttacatatat aacaccaggc caacagcaca attcagattc tcaggcactt
      361 tcaagcagaa caggaagctg actgtcacgg atatatcgaa tctggcgggc agcgatatcg
      421 tcaacagtgc ttatccgatc gactcctggt catggaagat cagggctgtc agcggggatc
      481 agtcatccat aaggaagaaa gatatcagca aaactcagaa ggatctgctg tttaaaaaac
      541 cgggttcgta cgaggtcgag cttaccgtta cgaatacgct gggccgtgta tctgagccat
      601 atatcataag gtttgagatt ttccccgact atgaaccggc actggaaata gatctcgaca
      661 attctgtgat ctccaggacg gaaacggtca gcgcctggaa ttataatgca tgcagtacgg
      721 acaatgacat aatcagtaaa aatatcatcg aactctggta cgacagtgac aatgacggag
      781 aatatgataa actgcttgag acatacgacg gaaggaacgg cttcccggaa ttccgtccgg
      841 ttgggcttgg aagatataaa tttgtcaaca cagtagagga aagtttcggt gaagatacga
      901 tcgaagagtt catcactgaa agcgatcgtg taaccaggac cctggagcgg gaaatactgg
      961 tggataacat acagcccatg gcaggcctgt atatcagtat gcctctcaac aggccgcaga
     1021 tcgacacatt tataatgctg gactccgggc ttgatgaaga ggatgtgaga tatataaagg
     1081 acaacaggat cgagatcaac aactctcttc gctcggccaa cattctttct aatgtggagg
     1141 tgtgggacct tcgcacatac aggtatgaaa tgccggcaag caagtctgtc cacacaggga
     1201 gcagtctgcc cccggaaaca gtggagtaca gcagcaacgg gtattatggg gtgctgaaaa
     1261 ggacaagcta tatcaacaac aggtatcaga cagatgaagg ttattatgtt acaaaagtgg
     1321 ataaaaagac cgttacggga acgctttcgg gatggtctga agctcaattt gtttataccg
     1381 ctgccggctg gagacaaatc tcgtcatact ccacagatca gccgacaatg acatataacc
     1441 aggacggtta ctacggcgtg ctcagtaaag tatcattcac ggtcgatact gataacggca
     1501 ggccttccgg atcgggcaaa atcggagata cgtatacgtg gagaaaaaca tacaccgggt
     1561 actacagcgg cgaagtgaca aaaacaatct caatttggat ccccaacctg gtatggcata
     1621 acgattatac cggattctat tccggaacag tcagtaaaga ggtaaaacag ccatacacag
     1681 acccgttcag acctacgtca caaaaatata tagtatatat agcaggagac agcctgcatg
     1741 atatgaatga tctgagcttt gcgctgaaca aaagcggcgc cagactgata ctgatcggcc
     1801 atgaaagtat aagacggcag gtcgggcatg accatttcat agagaaaggc agacctcttt
     1861 ccgaactgat gtcggaagcc ctggacataa tagccgatac cggagtcgcc gaaaaatata
     1921 ctcttcttgc agggttagac gatctgcacc tgagtgtcgc ggattttgac gaagagaacg
     1981 atccaatagc cgagagaaaa ttcttatatg tccatgaccc gaactatttc gacaatggaa
     2041 gcggcctgga acagttctgc gcagaacatt actctgacag tgacggatgg gttgacatct
     2101 tcctgacaaa actgaacaaa ccgggcaagt acaccatata cagaagggta aaggatgacc
     2161 cttctgagga tccggcgttt gacagctttg acagatactc aggtacaccg tttgtcgagg
     2221 tctatgctca cagaaagcct gtagccggtg ctacactgga ctgggattat gatcctgaag
     2281 agaatgtata ccggacgacc tgggttgaca actcctatga tcctgatcac cagtacagca
     2341 ggatggacaa aggaatagtc gaaagaaaga tcatgtacag ggaagcggga ggcatgtgga
     2401 actacacgat cccggataaa ctgaaaccag ggacatatga actgtattat tatgtaagag
     2461 accctgaaaa cacatggtcg gatcctttca tattgaattt cagactggaa gaggcaccgc
     2521 cgatacagtt cgacgcatct ctccgggcat tggacaatgc gttcagcctt tcggacatcc
     2581 ctgcgtctga aaaacttgag gcttatgata tccggacgcg ttacccgggc aatttgaaac
     2641 tggagatggc attattcaaa ggagccctga gggaatcaca ggtcaaaaca gtagttttcg
     2701 gaaacgatac gggtacaagg gtgaataatg atatatattg gaacaatgtg atatatacga
     2761 tccctgcaga ccttccggac ggaatatatg atttcaggat ttccgctgtg gctgatgatg
     2821 ggaaaagcgc tgcaaagagt tttccggtga cggtttcaac gccgctaaac cttgtccctc
     2881 atatgcccgc agaattgtca ggaggcgcga gagcgcatgt atccgctgaa acatccaaat
     2941 atgcgggtgc tgtcagcatt acactgttcc atggtactga acatgccagg acattcagcc
     3001 tgacagctcc ggccggtgcg gagggagcag ttaagcactg ggaaagcgtg gtttccgtac
     3061 catacgggat accagacggc gaatatacgg caagattcac ggcaactgcg ccgaatggca
     3121 agtcccagag caaggatgta cgattcggct taacaaacat agctataacc gacgtgaagc
     3181 tgagcggcta ctggaaccat tggaggggac aaactgacat cttcggcaaa aggatgactg
     3241 tggagccgca caggttcctg tcgctggagt gtgtcaaggt cgatatcagg accaagggcg
     3301 atccggaaaa agtagtgata cggttcagtc ctgaacttga ggcgatgcga tataccgatc
     3361 ccaacggcca cacctatgac tacggcaaag atttcttcgg ctaccaggtc aggttcccgg
     3421 aagattccac attgacagtc accgacaacc aggcacagtg ggagtattat ctgccgctgg
     3481 ctccttccac caaggattgg gacaaccgaa ggctcaggcc gcagtaccgt atgacggtca
     3541 cggtatataa aggggaaagc tttgctgaat acgtgatcga cgatatagac attaccggta
     3601 atgtctacga tttgacctat atccagccga aaagatgaca aattttagta gttctcataa
     3661 tggaaatgct taaacgggga atgctaaaat gaagttgtag ggaaataatc tgttgttcgg
     3721 gcatcagatg aaagaggtgc ggcatatgtc cattctgagg aatgcgaaga tattcgcagg
     3781 cagttccggc agggtttttg ccgaaaaaat gtgtgagttc atcgggggca ggctatcaga
     3841 atcggaagta atcagatttt ccgaagggaa tacgtatgtc aaggcatcgg agactgtaag
     3901 aggcatggac gtttaccttg tccaacccat cggtttgagg cccaacgacg aattcgtaga
     3961 gatactcttc tgggcggatg ccttcaaaag agccagtgcc aatacggtta cggtgataat
     4021 gccgtatttc ggctatgcta aggctgacaa gaaggatgaa cccagggtat cgatcagggc
     4081 aagggtctgt gcggagtgta tggagcttgc cggtgttgac aggatcgtaa cgatggacct
     4141 gcacagtccg caggtacagg gatttttcaa aaagccgctg gaccatctga gggcgcttcc
     4201 ggtgctgtgc gaatatatca gggcaactgt caatgatacc ctggtggtcg tgtcacccga
     4261 tgccggattt gcgaaacagg ccaggaaata tgcggattac ctgggtgctt ccattgcgat
     4321 aggtgacaag accaggcggg cccatgacga aaaagcgcag atcctcgacc ttatcggaga
     4381 tgttgctgga aaaactgcgc tgatcgtgga tgacttcacc ctcagtgccg gtaccctggc
     4441 cgatctgtcg cacttgctga aggagcgggg tgcggtcagg ataatagcgt gtgtcaccca
     4501 tctgctgcta agtgaaaaga gtgtagagag gataaacggg agtcccatcg atcttgtgat
     4561 cggaacagat tccgtcgaga acaggaacct gctgaaatcg gacaaagtaa ggctggtatc
     4621 ggtggcgccc ctttttgcac aggctctgat caggatccac aataacgagt cggtaagccc
     4681 gttgtttacg aatatttctg agaacgtaaa gtaccagtcg tacaggtgaa attaagttta
     4741 agcctggtcc cgcagacata ttgcatattc ttacggtata gtttagtgta agaaagtggt
     4801 gcacagaaag gcgggatcaa tatgtggacg agggccgaac tgaagtacag ggcaagggag
     4861 gctttgcgcg gcaactactg gaaggccttt cttgtcagcc ttgtgatcat gatcgcgacg
     4921 gaaggattag gaggcggttc aggaagagcc ggtcatgatg agatatcatc gtccggggga
     4981 agtttcttcg gcagtatcgg cagtattgag tgggccgctg taatcgcagc cattctgggg
     5041 ttttttgcga caatattcgt aatatcgctt gcactgcaga tatttgtgat gaatccgctg
     5101 gaggtaggcg gaaggaggta tttcatccgt actgctcagt acatggacaa cagcagatgt
     5161 ttcaggttcg ctttcaatgg gcacaattat ctcggtattg tcggaggtat ggcgctcaag
     5221 gacctgtacc tgttcctgtg gacacttctg ctcataatcc cgggaatcgt caagtcgtat
     5281 gcttacagga tggtcccgta tatactggcc gataatccca atataggagc gggaagggcc
     5341 atcgaactga gcgaaaggat gacatatggc cataaatttg atatatttat acttgacctg
     5401 tcattcctgg gctggtatct tcttggcttg ctttgcctgc tggtaggcgg tttgtttgtc
     5461 aagccgtatg agtatgcgac aaatgccgag ctttacctta ccttgaggcg gaacgccatt
     5521 gaaagcggtt attgcacccc ctgggaactt aacctgtatg atcctgtcca tgatgagtat
     5581 gatttatgat ataatttgtg acatgataac ttatgctgtc agggaggatg gtacaggtgt
     5641 ggctggataa actggaaaag aagataggga ggtactgcat caggaatctg atgctttaca
     5701 tcactacttt gaacggtgtg gtgttcgtcc tgacgtatct ggctcccgag cttgattttg
     5761 tgaacaagct gacgcttgtg ccctcactgg tgatgaaggg cgaagtatgg aggctcgtca
     5821 cgttcctgtt cattccgatg aatttcaatc cggtatgggt gatattttca ttgtattttt
     5881 attatatgat cggttcgtca cttgaacatg aatggggttc attcaagttc aatgtgtact
     5941 atttagtcgg ggtgctggga accatagcgg gcgctttcct tggaggattg gggacttcca
     6001 cgttcctgaa cctttccttg atatttgcct ttgcatacct gtacccgaac taccagctgg
     6061 tgattttctt cttcttcccg gtaaagataa aataccttgc aatattctat gccggcgggc
     6121 tcgtgcttac gttcatgctc tcgccgatca tcggcctgat cacggttttg ggctctgcgt
     6181 tcaacttcat actgttcttc tggaaggaca tatattacag actgaagttc aaccggaagg
     6241 cctatatgag ccagaaggag ttcaaggcga agataccgaa gatacacgtg atgcaccggt
     6301 gtacgatatg cggaaaaacc gacgtcgagg acaggaatat ggacttccgg tactgtgtgg
     6361 aatgtgaagg cgactatgaa tattgcatgg accacctgta caaccatgag catgtaaaag
     6421 caaacgcatc cgcaggcaat gacgacagta cgggaaatga cggcaacatg ggaaataacg
     6481 gcggaaatgc cagagcgaac tgataagtac ggatacaatc aaaaaccgga ataatatcat
     6541 caaaaaaccg gcttaaaact cagccggttt ttcattcgta atcccaaact cgtaaaggat
     6601 cgcttttact atccgctggg aggctttacc gtcgccataa ggatttacgg cctttgccat
     6661 tttttcatac tcggccggat catccagaag ctgcgaggca agagccttta tcctttcacg
     6721 ttcgatgcct gccagcttga cggtgccggc ttccacagcc tcgggccgtt cggtttcctt
     6781 tctcattacg agtaccggtt tgcccagggc gggagcctct tcctgcaggc cccctgaatc
     6841 agtcatgact atgtaggacc ttgccatcag gttgtgcata tcctggacat ccagggggtc
     6901 tataaggtgg accttcgggt ttgagcctag tatcctgaat gccgtttcac gtactgccgg
     6961 gttcagatgc acggcatata tcacgcatac atcgtcatat tcggaggcga tgtcgttcag
     7021 ggcgagacat atttgctcca gcgggctgcc gagattctcg cgcctgtggg ccgttacggc
     7081 tatgactcgc ctgcctctga tgtctatttt gcgcatttcg tcgctgttga aaacatagtc
     7141 ttctctgaca gtggtctgca tcgcatcgat gacggtgttg cctgttatgt aaattttgtc
     7201 cgggtttatg ccctcactaa ggaggttgga cttgttagat ggcgtagggg cgaagtgcag
     7261 gtcggcaagt gccgctgtga gcttcctgtt catttcttcc gggaaagggg agtatttgtc
     7321 gaaagtcctc agcccggctt caacatggcc gatgggtatt tttttataaa aggcggcaag
     7381 cgatgatatg aagcatgtgg aagtatcacc gtgcaccagg accatatccg gcctttctct
     7441 ttccaggacg accgagagcc tgtccagggt cctcgccgct acctgctcca gtgtctgccg
     7501 gtccagcatg atgtcaaggt cgatatccgg ttttatccgg aatatgtcca gcacctggtc
     7561 cagcatttgc ctgtgctggg cagtcacgca cacgaccggt tctatttcgg gacatttccc
     7621 gagctccttg accagcggcg ccattttcac cgcatctggt cttgttccga atatgggcat
     7681 tattctaagc tttctcattt ggttacccca tctgactgct tttttatatc agtatccggg
     7741 actgctggtt ttgaagggcc gtcatctgtc gtttttctgt catctaccag tccgcggtca
     7801 tttatgtatt tcgcgccgcc tgcgacgaaa atcgctgccg cgagcagcat gacgatcgcg
     7861 ctcaaagcgc ccctgtcggc aaggacgatg gcgcataggc ccaaagatgc gctggcggta
     7921 tataatatca ggacggactg cctctggctc agccccatat cgatgagcct gtggtgcaga
     7981 tgtcctctgt cggcctgcat tatggacttg tgcctgaaca gccttcttat gatcgcaaac
     8041 gccgtatcga acagcggcag gccgagcacc agcagcggta tcactatcga tattgcggcg
     8101 tacgacttca ttacgccctg gatcgaaata acgccaagcg tgaagccgag gaaagccgct
     8161 cctgtttcac ccaggaaaat tttcgccggg ttgaaattga atgggaggaa acccaacgtg
     8221 gatcctgaca gtgctgccgt tatcaatgcg atatatactg cttttgcagg gtccaggtct
     8281 gcccgcagca gcgtaacgaa aaagagagat agcgatgata tggaggaaac tcctgcagcg
     8341 aggccgtcca ggccgtcgat caggttgatg gcattcgtta ttcctactat ccatattatt
     8401 gtcagcggta tcgatatata tggactgagt tctatatatt tgtcatcacc gaacgggttg
     8461 gtcacaagtg atatccttgt ccccgaaatc gctactgtgc atatggcagc taccagttgg
     8521 aacgaaagct tggcccacgg cttcagagtc cttgtgtcgt cgagtatgcc catgaccatg
     8581 atgatcgcta taccggatac aagacccagc aactctttgc ccggagtaat gatgttggat
     8641 gcggtcgcca ggtcgaacac gagcgatatg ataaaaccgc agatgatggc aagtccgccc
     8701 atcagggcaa tgggcttttt atgcatcctg cggtcatcct tcggaacatc gacgccgccg
     8761 accttgaatg cgagtcttct tactatggat gttgctgaaa atgatattat aaaagctagc
     8821 gtgaatgtga ttacatattc agtaatatat gacaaatcgg acacacctgc ctatactctg
     8881 accactctta caccggcttc cttcagcagt tccatcgcca ggtcgtcggg gtagtcacct
     8941 ttgaaaacta ttttcttgat accggcgttg attgccatct ttgcgcacag tacacatggc
     9001 tggtgggtaa cataaagtgt accgccattc acgctggtac ctgagtaagc agcctgtacc
     9061 agggcattct gttcggcatg gatagccctg cataactcgt ggcgctgccc ggatggtatc
     9121 cccatttgct cgcggaggca gcctacttcg gagcagtgct tacagccgga tggcgctccg
     9181 ttgtatcctg tggcgagtat acgcttgtcc tttacgatca gcgctcccac ctgacgcctg
     9241 agacatgtcg atctttcttt gacaagttcg acgatcccca tgaaatattc gtcccatgat
     9301 ggtctcatgt tattgcctcc ccaaaataac catatcttga ccccgcaatg caacgggaca
     9361 tggtaaccag gccagtttcc gagagctgtc cctttttaag acgtcctttt ggatgccata
     9421 cccgcttttt gagaactgtc cccttacttg gtaccgaaaa gtctgtcgcc ggcgtcgccc
     9481 agaccgggga cgatgtatcc gtgatcgttg agcttttcat ccactgcggc gcagtagatc
     9541 tccacatcgg ggtggtcctt gtttattctt tctatgcctg cttttgcagc tatcaggcac
     9601 atcagtttta tgttcgacac gcctttttcc tttatgaacc tgatcgctgc tgaagctgaa
     9661 cctccggtcg cgagcattgg atcaagcact acgatttcgc gctcgtgagc gtcaacaggc
     9721 aacttgcagt aatactcgac cggctccagc gtttcaggat cgcggtaaag cccgatatgg
     9781 ccaacctttg ccattggcag caggttcagc atcccgtcca ccattccgag cccagccctg
     9841 agtatcggta cgatgccaag cttcttgccg gatatcacct ttgtttttgc tataccgaca
     9901 ggcgtctcta tctcaacttc cttaagcggc atgttccttg tgacttcgta gcccatgagc
     9961 atggcgatct ctgataccag ttccctgaat tccttgacgt ttgtgttttt gtccctcagt
    10021 aaggagatct tatgctgtat cagcggatga tcaaatacga atactttttc catatgcagc
    10081 acctccggtt atgtatgtgt ttttgaatac ttactctcaa tgtcggcgaa cttgtccact
    10141 ctttgcttgt gtcttcctcc caaaaaagag gcgttcagcc aggcatcgac tatatcgatc
    10201 gcaaggccta cgcccaaaac ccttgccccg agggcaagaa cgttcgcatc attgtgctcc
    10261 cggctcatcc tggccatgta tgtatcggta cacagagcag ctctgatgcc cgggactttg
    10321 ttggctgcta tggaaatgcc gatacctgtt ccgcagatta tgatacctcg ttcgtgggtg
    10381 cctgaagcta ctgcctcggc caccttgagc ccgtaatcgg ggtaatccac ggatgtagta
    10441 cagtcacatg taccaaaatc ctcgaacggg acgttgatct cttcaagatg ctttattatc
    10501 tcgcttttca ggctgaaacc tgcatgatcg cttgctatag ccaacatagt accaccctcc
    10561 tgccaacttc ctattcaatt ttatattatc gatatattaa agtcaaagcg ctatcgacta
    10621 ttttaacttt tctataagtt tttcgacagc ctgcgataat atttcggcac aatgcttata
    10681 taccgactca tcaccgccgt aaggatcagt tatgtctccg tcggtcccac agacatattc
    10741 tttcaatgtg aagacttttt gatacgcttc cgggtacatt gacagaatgt actgcctgtg
    10801 gatcctttcc atggtcagta tcagacaggc ttcctctata tctgcgtgag acagtctctt
    10861 tgatgcatgg tatgacagat cggcgccggg ataaccttcc attgctttta tcgcgttgat
    10921 tgacgccggg ctgccttctg cggctgccag gccggctgaa gcagccgtat aaccgatacc
    10981 ggccgttgcc gccagacggt tgaacagcgc cgctgccatc gggctcctgc atgtgttgcc
    11041 tgtgcatacg aaaaggatct tcttcatgac gactccctta cagttttact atattaccgc
    11101 ccgctgcctt cataagcctg ttcatgactg cctgtccgat tcccctgaca tcggtggttt
    11161 cggccattat cacttcgacg cctttttcgt cgaattccct gaggcagcgg aacaggtttg
    11221 cagcaagagt ctttggttcc tttctgctgc cgagagagag tatcttgcag taggaataga
    11281 gtgaagattc atacagcccg gctgtctcgt ctgtgacgag tatgcccacg gctgtgcctt
    11341 ccttatgata tagccccgct cttttgctta tttctgcggc gaccttttcc ggatcgccct
    11401 ggatcaggag catagttgcc ttcggagaat aatgcctgta tttcatgccg ggagatttcg
    11461 gcgtgccggc catgtcggag atacccggcg tctggtcgct gatgacattg cccaatacat
    11521 gcctcagctg ctcaagcgtg actccgccgg gcctgagtat aacaggcggg tcgaccgtga
    11581 tatccagcac tgtggactct acgcctactt ctgcgttgcc gccgtctatg ataacatcta
    11641 ttttccccat catgtcctcg atgacatgcc ttgcaagtgt cgggcttggc ctccctgaac
    11701 tgttggcgct cggggctgct accggcactc ctgctttttt tataagagcg gatgcgatgg
    11761 ggtgggaagg catcctgacc gcaactgtgt ccagacctgc cgtgacgata tcgggtacca
    11821 gcgaagacct tggcatcaca agagtgagag gaccaggcca gaacgcatcc atcagcgcct
    11881 gtgcagttcc agggatgctg tccgtcaggt cttttaccgc ggctgtatct gctatatgta
    11941 ctatcagcgg attatcagaa gggcggcctt ttgccctgta gatggatttg acggcctctt
    12001 cgtccagcgc attggcgccc agaccgtata ccgtttcagt agggaaggcg accaggcctc
    12061 cggatctcaa tacctctgca gcctgatcca gttgcttgtc ccaattgctg gtgctagtgt
    12121 ctatctttat tatctgtgtc ttcaagctat gcgactccct tttcctttgc cattgataat
    12181 gtcgtttgat acgggaaaat tatcctgtca ccgtaaattt tcccttacct caggtgttat
    12241 aaaccgatat tattatacca cataatatcc ccaaaacatt tgccaatgac gacagtcttc
    12301 cgagataaag ccgtttcgat tcggggacca gttcgccgta aatgatatac aacattgctc
    12361 cgccggcgaa tccaaggcag gctgcggtca ggctgtccga gacagatccg agcactgcgc
    12421 caagcagtgc tccaaggccc atcggcacgc cggaaagcac cgtatacgca aaggccttga
    12481 actttgagaa gcctcctgct ctcatcggga cagccatggc gatcccttca gggatgtcgt
    12541 ggatgattat cacggcggtg atcgttactc ccaggctggt ggaagcttca aaacccgaac
    12601 ctactgcaaa accctccggg aaattatgca tcgcaatggc tattgccatc aggataccgg
    12661 cccgaagcag tcccgtattc tgacctttcc ccagcgaccg ggatttcttc agaaagcttt
    12721 cgatagcaac taccgtcagg ataccggtga ttatgcccaa aaatgtgaaa acagtccctc
    12781 caaggctgaa tgcctccggc agcagttcaa aacagactac tgcggtcatc agtccggctg
    12841 agaactccaa tatggtgctc atgatacggt tgctcgtctt tgagatcaga aacgccgaaa
    12901 ggccgccgat accggttccg gcaagacctg aaaacaatcc caggaatgtg attttgagca
    12961 agtgtcccat acagccctcc agtactgccg atcagtatcc gttcgattta tgggccaata
    13021 ttgtatggca gcagcccggg caaaccaata catgcgattc aggccggcgc aaaaccaatg
    13081 ccggtatggc tgtggttcac ataaacggat acatgcacga cttgtgtgac atttaaatgt
    13141 ttattcacag gagggcttgt attattagta ttttggtcaa tcttcgccgc cggcggcgct
    13201 gccaagtttt tgggactggt ccgccgttat cagcgcatcg atgagttcat cgagatctcc
    13261 gtccattatt tcgccgatct tgtaaagggt aaggcctatc ctgtggtcag agaccctgcc
    13321 ctgcgggaag ttgtaggtgc ggatcctttc gcttctttcg ccggtcccga cctgggattt
    13381 gcgggtctgc gctatttcag actcctgctg ctgctgggcc atctcgtaaa gtttcgaacg
    13441 gaggatcctc attgccttgt cccggttttt atgctgtgag cgctcatcct ggcatgtcac
    13501 gactatgccc gtgggtatgt gggtgatcct tattgccgat tcggtcttgt tgacatgctg
    13561 gccgcctgcg cctgatgcgc ggtatgtgtc aatctgcagg tcgttcggat ctatctgtac
    13621 atcgacttca tccacttcgg gaagaacggc cacagtgact gtggaagtat ggatcctgcc
    13681 gccggcctca gtgaccggta cgcgctggac gcggtgcact ccgctttcat atttcagcct
    13741 gctgtaggcg ccttttcctt ctattgaaaa tatgacttcc ttgatgccgc cgatctcggt
    13801 ttcatgtata tccagtatct ccgatttcca ctgatgcctt tcgcagtatc tcgtatacat
    13861 cctgaacagg tcgcctgcaa aaagggcagc ttcttctccg ccggctcctc cgcgtatctc
    13921 gacgatgacg ttccggttgt cgttgggatc tttcggtatg agcagctgct tgagctcatt
    13981 ttcgatagtt ccaagctttg ttttcgcttc gtccagttcg ctctggacaa gctcgcggaa
    14041 ttccttgtcg gagttttctt ccaggagttc ccgcgtctct tcgatatctt tcttcgtctt
    14101 cttatattca cgatatttgg atacgatctc ttcaagcccg gaaaactctt tcatcagctt
    14161 tctgtactcg tcctgatccg ctatcacctg gggatcgctg agcttcatgc ttatctcttc
    14221 atacctgttt tccgctgcat caagcttgtc aaacatactg cacctcatga ctaaaattat
    14281 cggtgtttta ttatagcata gaccggatgt aatatctata tagatcggtc cgtgatgcat
    14341 tctttgtaat gctcctggtt tcatgccgtt acaggctgcg gcagaaatct tgcggcgttc
    14401 gtacaaaaaa ggcaggacaa aacaggccgg ataaaggaaa acgggccggg caggacattg
    14461 cagcctgccg cggcccgttt cgattgttgt ttgtgatcaa attatcggat atggagggtt
    14521 tcgccatgac aatttgatca caacgtgctt ataccgtggt gatttgattg tttgcggtta
    14581 tttgatcact tcgatgtagc cgtttgatgt ttccgcctgg atattgacct tctgggcggc
    14641 tgcagcgtag ttctcggtct cggcgtctgc ctgcctgttg aacctgtctg tcctcattgc
    14701 attgcggtaa aggagattcg gtatcagcag gtttatatcg ccgttcgagg tccttgcact
    14761 gatcttgtag cctttattct cagagtcgtt catattggcc ttgatgttgg catttgatgt
    14821 cctgagattc agttcggcca cattctcgtt gtcgtagttc tgtacattgt cgaccaggat
    14881 cttgccgttg gatgttaccg ctgtaagctt gacagccttg aggttcttga tctcgatagt
    14941 agcgtttgat gtgccgaaat tgacatcccg gccgatgatg taatttgcgc ttacctttgc
    15001 gttcttggtc tcggcggcga tcctgccgct gtttacgccc gtcaggtcga tgactgcatt
    15061 ctttgtaacg ctcacaaatt cttcagatgt tacgtcttcc acatagatct tgccgttctt
    15121 ggtctcaaga gcgacctggt tgaatttcac agcgggaaga agaacatcgt atgaaacgct
    15181 cagattgtaa gtatccggtt tggccagctt tactgaaaca gttccgttct cgttggagaa
    15241 tgcaatggcg tttttggcat tttcctgcgg tgtgcggatc tttgccgtta tgagtatttt
    15301 gtcttcctgg tgttttttca ccgtgacctg gccgttcgtg gcctggagtt ccaggttcat
    15361 gccttctgcc gccgccactt catactgctg ctcttctgtc acgcaactgc cgaagatatt
    15421 gaatgcgctc gtgtcgatga tgcttccgat gaaatccgcc accttgtcgg cgatcccgaa
    15481 tgcggccgtg gcgacatttt tgccgaattt ctcagccttg gcgctgaact cttcgaccat
    15541 cttgtcaaag tcgttctgat tgtaattttt ggagagttcg cgcctccact catcgatcct
    15601 ttcccttacc tttgccacct catcatagta gttgcgctgc ggcggtttcg gtccatggga
    15661 tcccctggga ccggtaccgt aggaggaagg ttcctttttg gcttccagtg cttcgagcag
    15721 ctttgctgct tcttcgctgt tgatcctgcc ctcctggagc attttaagta tcatcatttt
    15781 ttcttcactt atggacattt tgatacctcc tgtttgataa tgttcgtcga tcatgttatt
    15841 tcaacaactc tagtgcctct tccactgaaa tcagcccgtt atcgagcata tcaagtattt
    15901 ccatcctttt ttggctttca gggttttcat agctttgcat gccgtatccc aaagcgtttg
    15961 cggcgtcttc gagcctgctt ttcactgtcg ggtaagaaat gcctagttct ctctccacct
    16021 ccttgatatt gcctctgcat ttgataaatg tttcgatgaa tgatttctgt tccgctgtca
    16081 gcctgcagaa cctgcacagc gtgaaatgtc cttcgatttt tgtatcgcag ttgctgcaat
    16141 atattgcagt tacttcagta tcctgtccgc agaccgggca cctgcccaat gcctctttct
    16201 tcataataag cactccttat acgcaagatg tattatgtgc attaagatta ttaaagtgca
    16261 tattaattat tttgaccttc cattttatat tttatattta cagctttttc ttgtcaaggt
    16321 tcgtcgggca agtaccttaa aaatatcaag ccatatctta atttatttaa tgtttcaata
    16381 aattatacgt gataataagt ttattgtctc actaatttta aaatttattt tagtttgccg
    16441 aggccgataa tgcagccagg aaatgcgaac agtggcgagg tgatcccggt aatacagacg
    16501 agagcaaacg gccgttctgt tactgaactc caggtagatt tcgttgtgga cggtgaaata
    16561 gagcaggtgc cgatgtttgt ggtaggggat ctaaggacgc aggatacgct ggatgaagta
    16621 cgaatttatt ttcattgctc caatgtaccg ggtcttcagg cctacaggaa acctatgttc
    16681 aaatcggcgc acctcgaaat gggagatccc ggactattga ccggcgctgt aagagaatat
    16741 tatgaagcgc tgcaccgggc tccttcttta gacgtcgaca ggataattgg ctgtatgagc
    16801 gagaactgca tatttggcgg ttatgaacct tacaattcgg atcacaagcc ggccgccacc
    16861 ctggccgaaa tcagggaaaa atacgagtat atgtcgaggt atattccaag atgcgttgga
    16921 atgcgttatg aaactataat cgataacggg cgcacctgtg tcatagagtg ggtgcatatc
    16981 gtatcgagag caggacagga agaaatggcg agagtcgcaa tgtccggtat tgccgcatat
    17041 gaacgtggtg atgacggcct tctctgttcg atccgtatat gtgattatgc gggactggag
    17101 cgtacgatag actggactaa aacccctttg acaaaagagg aggcgcaaag gataaatttc
    17161 gtagaagaat ttccgcaggg tgtcggcttg aaaaagtaat acgttgatgg tgtctgcgaa
    17221 gagacactta ccgccggacg cttttactga aagataacag gagttttata cgggcaatgc
    17281 cgtcaaactt tttcacgctg tctatgcaaa aataaacggg ccgattctgt attgcccgtt
    17341 tatttttata atttagctac cgtgttcaga ttttgaatag ttttatgcac ctcaaacaaa
    17401 cttctttccc ttttcaaata aagtcctttt atagacag
//
LOCUS       DAOSCC010000017        17431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2255_length_17431_cov_4.525725, whole genome shotgun sequence.
ACCESSION   DAOSCC010000017 DAOSCC010000000
VERSION     DAOSCC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17431
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_17431_cov_4.525725"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /gene="hisB"
                     /locus_tag="PLG72_01930"
     CDS             <1..306
                     /gene="hisB"
                     /locus_tag="PLG72_01930"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634832.1"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOJ79594.1"
                     /translation="ASVPMDEALAMVSVDLGGRPYLVFNVKFSGELAGSIETGLIEEF
                     FRAVSVNAGMNIHINVPYGSNNHHICEAIFKAFGRALDEASRTDSRVKGVMSTKGVL"
     gene            387..1100
                     /gene="hisA"
                     /locus_tag="PLG72_01935"
     CDS             387..1100
                     /gene="hisA"
                     /locus_tag="PLG72_01935"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOJ79595.1"
                     /translation="MVIYPAIDIRDGKCVRLVQGRFDDVTVYSDDPVEAARSFVRAGA
                     QYLHVVDLDGARLGSPQNADIICAIAAQTGIPVQTGGGIRTAETMEMLLNRGIKRVIL
                     GTSAVNDPTLVKKAAEKFSGRFAVAIDAKDGFVATEGWERTSGFTAVGFARKMEEFGA
                     GVIIYTDISRDGMLKGPNLKAMEEMVKAVGIDVIASGGVTSIQDIKNLKDAGVAGAII
                     GKALYTGNIDLGEAIEAAK"
     gene            1270..2031
                     /gene="hisF"
                     /locus_tag="PLG72_01940"
     CDS             1270..2031
                     /gene="hisF"
                     /locus_tag="PLG72_01940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ79596.1"
                     /translation="MSTKRIIPCLDVIGGRVVKGVNFVNFRDAGDPAENAMFYEKAGA
                     DELVLLDITASAEGRDTIVDVVRRVAGCISIPFTVGGGIRTCEDFKRMLEAGADKVSV
                     NTAAVKRPELISEAAERFGSGRVVVAVDAKRREDGNGWEVYIGGGRVNTGLDAVEWSA
                     RATELGAGEILLTSMDRDGTKDGFDIELTRAVAENTGIPVTASGGAGSKEHFYDALTE
                     GRADAALAASLFHFREIDIRELKHWLAEKGIEMRV"
     gene            2042..2746
                     /gene="hisIE"
                     /locus_tag="PLG72_01945"
     CDS             2042..2746
                     /gene="hisIE"
                     /locus_tag="PLG72_01945"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOJ79597.1"
                     /translation="MPDMVKFNRDGLVPVIAQDVRTDEVLMLAYMNEEALNETIRTGM
                     AHYYSRSRQKLWLKGETSGHYQKVRSISIDCDGDALLIKVEQTGAACHTGHRSCFFTK
                     FSSSGQGGTPGTAETAGSRTTAADAAGSGGCGSDSTAGHTGPEILTEVFSVILDRKVN
                     PKEGSYTNYLFAKGLDKILKKVGEEAGELIIASKNGSPSEIRAEAADLFYHIMVLLAE
                     RGMSLSDVFDELKNRA"
     gene            2935..3222
                     /locus_tag="PLG72_01950"
     CDS             2935..3222
                     /locus_tag="PLG72_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ79598.1"
                     /translation="MTIKPLGERVVIKMLETEEVTKGGVVLPGSAKERPQVAEVVAVG
                     PGGYDDKGREIKMEVKVGDKVLISKYAGTEVKIDDVEYTILKQSDILAKVE"
     gene            3281..4906
                     /gene="groL"
                     /locus_tag="PLG72_01955"
     CDS             3281..4906
                     /gene="groL"
                     /locus_tag="PLG72_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ79599.1"
                     /translation="MAKEIKFSEDARRALERGVNQLADTVKVTLGPKGRNVVLDKKFG
                     TPVVTNDGVTIAKEIELEDKFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     MKNVAAGANPMILKKGLARAVEVAVDAIKENSRKIQGKEDIARVAAISANDEYIGELI
                     SDAMEKVTNDGVITVEEAKTMGTSLEIVEGMQFDRGYISAYMVTDTDKMEAILDDPYI
                     LITDKKLSNVQDLLPLLEQIVQAGKKLLIIAEDVEGEALTTLVLNKLRGTFISVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISDELGLDLKEVKLSQLGRARQVKVQKENTIIVDGA
                     GKPEEIKKRISSIRNQIEETTSDFDKEKLQERLAKLAGGVAVIQVGAATEVEMKEKKL
                     RIEDALAATKAAVEEGIVAGGGTAYVNAIPKVAKLLNEVHGDEKTGVQIILKALEEPV
                     RQIAANAGLEGSVIVDKVKNSPVGVGFDVLKEEYVDMIKAGIVDPTKVTRTALQNAAS
                     IASMVLTTESVVADKPEKEPPMPAGGGGMGGMY"
     gene            5266..6591
                     /locus_tag="PLG72_01960"
     CDS             5266..6591
                     /locus_tag="PLG72_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ79600.1"
                     /translation="MDDRIFYVAAGIIAVSAIIISGYAKNKRRVRKLEQELVGQWGRA
                     PEGKYKDTDMKAIAGFFRELSRNSGGDFLVDDITWNDLDMDRIFMRINNTQSTVGEEI
                     LYSLLRRPVFDGSELMERGRLIEYFRTNPDECLELRKILAALGKKRHAQVYEYFFGEP
                     VTFRYGRYVLQAAALLFSPLLMAIDVSFGFAAIVGLFIFNMLAYHKAKSEYEIYLAGL
                     CYLVDMIRCSKKIAAAEIPGIKEYAARMGNLSGEMRSFSINSFYQLFYQTGDYMVFEP
                     LKSMFLLELIAFGRLLKTIYAHKQVLREMYETIGYLDSMVSAASYRESLDFWTVPALY
                     TSDGAKPVSLSIRDACHPLIENAVANSIDAVRPVLITGSNASGKSTFLKTAAINAILA
                     QTIYTCVARGYRSCYFKVFTSMALLYSRDQLAEKDTGQPGRTGTAAVRH"
     gene            6575..6931
                     /locus_tag="PLG72_01965"
     CDS             6575..6931
                     /locus_tag="PLG72_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79601.1"
                     /translation="MLCVIDEVLRGTNTIERIAASTEILRHFAGGNCILIAATHDIEL
                     AEILKNLYDNYHFTEKYEGDEIKFEYKMHPGKSTTHNAIKLLKVIGYPEHIVKNAEDR
                     AEKFLAEGRWHTVNAE"
     gene            7036..7857
                     /locus_tag="PLG72_01970"
     CDS             7036..7857
                     /locus_tag="PLG72_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ79602.1"
                     /translation="MAIIAAGLFILTIFAAFYDGLSVRRYRIGTGKLERGQNVRIVLI
                     ADLHSSIYGENQEHLVNLVKRQNPDLIALAGDIADDDAPHAGTEMFLAGIRDAAPVFY
                     VSGNHECWSREIGEIKDMIRGYGVTVLEHSCTTIAVNGARIIICGVDDPDIRLEKGTG
                     YDWKQEMHEAFDSLADEPGYKILLSHRPERVNTYTEFSFDLVLSGHSHGGQVRIPFIL
                     NGLYAPDQGWFPKYAGGVYRHGSMTHVVSRGVSYNPGLPRIFNPPEVVVVDIYGK"
     gene            7954..8466
                     /locus_tag="PLG72_01975"
     CDS             7954..8466
                     /locus_tag="PLG72_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HOJ79603.1"
                     /translation="MDTFMEKLVTRKKTLTDHLITIGAVVGSALLILLALSIPILIQL
                     GLSLVLAAAAAYLGYRVITSRNIEYEYIVTNGDLDIDMIVSKRKRKRIFSANCKEFDI
                     VSPVKSSHFDQSVQSIKNRIDASSSIDSPDAYFITLNYNGERTLVIFEPTEKMLNNFR
                     LYIPRKVFRS"
     gene            8476..9135
                     /locus_tag="PLG72_01980"
     CDS             8476..9135
                     /locus_tag="PLG72_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ79604.1"
                     /translation="MPFELEILARLLLAVLLGGFIGYEREHTNRPAGFRTHILVCAGA
                     ALVMATSEYMVRRYSISAVDPARLGAQVISGIGFLGAGTIIRDGVNVRGLTTAASLWA
                     VSCVGIAVGCGFYLGAVIATILIFITLITLKKAERRFSRRNRFRTIIVESENISGQVG
                     LVTNLLEKNSIEIKNIQLYKSKDSAQMIKLLVKLPGGAMDVQTLNELQAIEGVREVYE
                     E"
     gene            9448..>11430
                     /locus_tag="PLG72_01985"
     CDS             9448..>11430
                     /locus_tag="PLG72_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ79605.1"
                     /translation="MMREDAEAALGLSSREAERRLLEYGPNLLTEKKKVSPVKLMLDQ
                     FTDIMVIILMVSTVISAFMGEITEAVTIIAIVTLNAVLGFIQEYRTEKTMEALKSLTA
                     PEAKVIRDGRQVSVPADRVVPGDIIVLEAGDRIAADAVLLDSVNIQADESLLTGESVP
                     VEKTAVSPGKDPARAGRNSRVFMGTSVTAGRAKALVTATGMNTEMGRIADMIQNIEEE
                     QTPLQKRLDKLGKIIAAGCLAICAVVAGVGIIRGEDVFNMLLSGISLAVAAVPEGLPA
                     LVTISLALGVQRMMRRNALIRKLPAVETLGCAGVVCSDKTGTLTQNRMTVRQIYADGR
                     IIGVADEKKVGRRFDKNPEGYFHVNGRVLDPTDRALRRVLEIAALCNNAVPAGETGLK
                     LPSPDVPASRSGHADKMRKFAGDPTETALMAAAEKAGLSFEELGMIYRRTDELPFDPE
                     RKCMSVVCVDRNGEAFLFTKGAPDMLLKKCSRVERAGTVKTMDHMDRSGILKANDRMA
                     AGALRVIAVAYKRLGKGRYSRNGLETDLIFAGLLGMIDPPRPEAAEAVMKCKMAGIRP
                     VMITGDHKLTAVAIAKELDIYGEGDTVLTGEELDSMDEESLEKAAATTTVYARVSPKH
                     KLAIVRALKRLGSIVAMTGDGVNDAPALKRSDIGVAM"
     assembly_gap    11431..11440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11441..12193
                     /locus_tag="PLG72_01990"
     CDS             <11441..12193
                     /locus_tag="PLG72_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ79606.1"
                     /translation="PAVKEADIGVAMGRTGTDVTKEASSMVLMDDNFATIVAAIEEGR
                     VIYSNIRKFIRYMLSCNLGEVLTMFLGMLAGLPVPLLPIQILWVNLVTDGLPAVALGF
                     DPPEKDIMKQRPRDPEESIFSQGLAGLIIVRGILIGLATLAVYASLLWYTADIGLART
                     GGFVTLVLSQLIHVFECKSETRNIFRIPLFNNIFLVLAVICSSVMMLGVIYIPVLQNI
                     FKTVPLGLNEWMIAGGLSFLAPVLTSMFMPRR"
     gene            12627..13574
                     /locus_tag="PLG72_01995"
     CDS             12627..13574
                     /locus_tag="PLG72_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79607.1"
                     /translation="MIEIQNLTKRYGQITAVKDLNFTVEKGEILGFLGPNGAGKSTTM
                     NIITGYIPSTEGTVKVDGYDILESPKEVKRRIGFLPEQPPVYMDMTVADYLDFVADLK
                     KVDKKQKRSQISDIMELVKIGDHRNRLIKNLSKGYKQRVGLAQALVGSPDVLILDEPT
                     VGLDPKQIIEIRKLIKALGKQHTIILSSHILPEVSAVCERVVIINKGEIAAIDTPENL
                     SRGLGSASKLTATIAGPRSSVENAIREIYGVKYVESAADKGKEAISYIIESDKDVDIR
                     KPLFFAMAKLGYPIIELKSLSLSLEDIFLQIVTEEKEVG"
     gene            13578..14282
                     /locus_tag="PLG72_02000"
     CDS             13578..14282
                     /locus_tag="PLG72_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79608.1"
                     /translation="MAAIFWKEVKSYFYSPMAYILIGLFILLTSIMFLPYLRYGYGLF
                     NDVLGTMGFILLVVVPILTMRILAEDRKNGTEVLLITSPVNITSIVVGKFLAVYFVFL
                     VMTTLTFVYPIVQLAFGAKFTVQLIGGYIGFLLLGATYIAVGVFASSLTENQVIAVII
                     SYVSLLIMWLADSVSSLVGGFVAKVLNWFSILSRYDDFNRGILGLSPVVYYLSFTAVF
                     LFLTVRVIEKRRWSQG"
     gene            14288..15751
                     /locus_tag="PLG72_02005"
     CDS             14288..15751
                     /locus_tag="PLG72_02005"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOJ79609.1"
                     /translation="MAKIDKKKIKNFLNKKSLKYGTNAAVITAAVIVMAIVLNLLVGL
                     LDLKLDLTPNKLFSLSEATVNILNELDQDVEIIGLFDDGAISSNDEYKQVTDLLNLYA
                     KYPHITVRYVDPDRNPGIISQLDPDDTLDLGSTDFLVRSTVNGAEKKKKLEYYDLFEL
                     EYDQYSISSYITGSNAEQGFTGAIKYVTSEYTPVVYFTEGHDEYDVDYYYRTLKGYLE
                     KNNILVKKINLLTVDQMPEDAELVVVASPKKDFQYAERDVLERYFNVGGKAIFMFDYL
                     KNDPSFDELNKLLNKYNLEINYDKVKETDDSRHLAQDPYTLVVDVKSNGIVSRAFNTF
                     LTNCRSISILKNEKEYITTTSLISTSPTAVGEMVNKSRGDDLPGPLELAVAVEYKGGE
                     KPSRIIAMGNSSFVTDSAYQVYGDFYFSNINFFLSAVNWMIEIKDEVIVPTKSYDVNR
                     FNITGQQATVMTWILVVVFPLLILGTGLMVYLRRRHL"
     gene            15748..17148
                     /locus_tag="PLG72_02010"
     CDS             15748..17148
                     /locus_tag="PLG72_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOJ79610.1"
                     /translation="MKFYRNAIILVVVVALLAGAYFLVRTKKANQQSDVAKKYDKLTD
                     YISTDIESVTLKNSDGTFVIVKKDDEWALSTPTDLRYDSSQLSSIVINSAAIVADKMV
                     EENAEDLSIYGLDDPATAIIKAKDGTSVTLEIGNQTPTGGGYYVKRAGENKVYTISKY
                     TGDKLTSGRNDIRTKQLFDITPDDIQKFGLNRKGQNIFISEKIDDTWNMVSPIKGSMN
                     DTSFLPMLEAVAGAEVVDFVDDDPEDLSVYGLDKPTYELIFSTEATGEIKLQMGLELK
                     NDSTIYAKLDGNDEVFTISTSPFTFLDKPLKEIVNVFAYIVNIQDVRQIDLTMDGKTT
                     HMDLDVYLDENGNMDSDKDKFYVNGVDASGRNENGKQPFRQFYQALIGISIDEIDVDG
                     DPSGGKADITIDYTLKDGKMKVEFIPKDENFYYVVRNGEYAGVLVKRKNKGEFGVDGM
                     KEAYNTMMEFLAGQQE"
     gene            17244..>17431
                     /locus_tag="PLG72_02015"
     CDS             17244..>17431
                     /locus_tag="PLG72_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79611.1"
                     /translation="MDDERRNILRVAGIYAASITGAGFASGQEIMQFFSVYKTGGFCG
                     ILLAGSLFSVLGCIVLGKV"
BASE COUNT         4820 a         3990 c         4942 g         3669 t
ORIGIN      
        1 gccagcgtcc cgatggatga ggcgcttgcg atggtcagcg tggatctcgg cgggcggcct
       61 taccttgtgt ttaatgtaaa gttcagcggc gaattagcgg gaagtatcga gaccggactg
      121 atagaggagt ttttcagggc ggtgtccgtc aatgccggca tgaatataca tataaacgtg
      181 ccgtacggga gcaacaacca ccacatatgc gaagccatct tcaaggcgtt cggaagagcg
      241 ctcgacgagg cgtcgaggac agacagccgg gtaaaaggag tcatgtccac caagggagtg
      301 ctgtgacacg gttttggcgc cggacagcag ccgaatggat ggcagcgcaa ggcttatgac
      361 aatgcatgtg atcggagtga tggcggatgg tgatttaccc tgcgatagat atcagggatg
      421 ggaaatgtgt gcggctggtc cagggaaggt tcgacgatgt gacggtatat tccgatgatc
      481 ctgtggaggc ggcccgcagc tttgtacggg ccggtgcgca gtatcttcac gtggtggacc
      541 tggacggtgc caggctgggg agcccgcaga atgcggacat aatatgcgca atagcggcac
      601 agacggggat accggtgcag acgggcgggg gtatccggac cgcggagaca atggaaatgc
      661 tgctgaacag gggcataaaa agagtcatac tcggtacttc ggcagtaaat gatccgacgc
      721 tggtaaaaaa agccgccgaa aagttcagcg gcagatttgc ggtagccatc gacgcaaagg
      781 acggcttcgt cgcaacggaa ggatgggaaa ggaccagcgg gttcacggcg gtcggatttg
      841 ccaggaaaat ggaggagttt ggcgccggag tgataatata caccgacata tcaagggacg
      901 gaatgctgaa aggccccaat ctcaaagcga tggaagaaat ggtgaaagct gtcgggatcg
      961 atgtgatagc ttcgggcgga gttacgagca tacaggacat aaaaaacctg aaggatgccg
     1021 gagttgcagg agcgataatc ggcaaggccc tttacacagg gaatatcgac ctgggggaag
     1081 ctatcgaggc ggcaaaatga atccggcagc atactgcctg tgccaactgc ggatttaccg
     1141 cagcttcgaa tgatgatccg ggaaaagata cccggggccc gaaggaagga cacatttgat
     1201 acgggatcga tgaaagcccg tgtcatgtgt gaaaaatatt cgtaaaatca gaatgagaga
     1261 ggtcaaaata tgagcactaa aagaataata ccatgccttg acgtgatagg cggcagggtc
     1321 gtcaagggag tgaatttcgt caacttcagg gatgcggggg atcctgcgga aaacgcgatg
     1381 ttttacgaaa aagccggggc agacgagctt gtattgctgg acataacggc gtcggcggag
     1441 gggcgtgaca cgatcgtaga tgtggtcagg cgcgttgcag ggtgcatttc gatccctttc
     1501 acggtgggag gcgggatcag gacctgcgag gatttcaaaa gaatgctcga agccggggcg
     1561 gacaaggtct cggtgaatac cgccgccgtg aagaggcctg aactgatatc ggaggcggcc
     1621 gaacgcttcg gaagcgggcg cgtggtagtc gccgtcgatg caaaaaggcg tgaagacgga
     1681 aacggctggg aagtgtatat tggcggagga cgtgtaaaca ccggcctgga tgcggtggaa
     1741 tggagcgccc gggcaacgga gctcggcgca ggagagatcc ttctgacgag catggaccgt
     1801 gacggcacga aagatggatt tgatatcgaa ctgaccaggg cagtcgcgga aaatacgggc
     1861 ataccggtga cagcgtccgg cggcgcaggc tcaaaagagc atttctatga tgcgctgaca
     1921 gaaggcaggg ccgatgcggc gcttgcagca tcgctgttcc atttcaggga gatagacata
     1981 cgtgaactga aacactggct ggcagaaaaa ggtatagaaa tgagggtgtg aaatgacgaa
     2041 cattcccgat atggtgaaat tcaacaggga tggtctcgtc cctgtgatcg cacaggatgt
     2101 caggactgat gaagtgctga tgctggcata tatgaatgaa gaggcgctta atgagacgat
     2161 aaggaccggc atggcgcact actacagcag gagcaggcag aagctgtggc tcaaggggga
     2221 gacgtcgggc cattaccaga aggtcaggag catcagtatc gactgcgatg gagacgcatt
     2281 gctgataaaa gtcgagcaga ccggcgctgc ctgccatacg gggcaccgtt cctgcttctt
     2341 tacaaagttc agttcatcag ggcaaggtgg gactccaggt acggctgaga cagcgggcag
     2401 caggaccacg gccgctgacg cggcaggcag cggaggatgc ggcagtgaca gcacggcagg
     2461 ccatacaggg cctgagatcc tgaccgaagt attcagcgtc atccttgaca ggaaagtcaa
     2521 tccgaaggaa ggctcctaca ccaattacct gtttgccaag ggactcgaca agatactcaa
     2581 gaaagtcggg gaagaggcag gcgagttaat aatagcttcg aagaacgggt ctccttcgga
     2641 aatacgggca gaggcggcgg acctgtttta tcacataatg gtcctcctcg cagagagagg
     2701 gatgtcactg tccgatgtat tcgatgaact gaaaaacaga gcgtaagaat gaaaaacggt
     2761 gaaacaaggc ggattatcca tgtaatttaa aatatcggcc caaaagcggc acattttaga
     2821 aaatccgcat atttgattaa aaaatgggta ttatataata taattagtac gattagcact
     2881 caacattagt gagtgctaat aaagtgcgaa aaaatataat aggaggtata ctttatgaca
     2941 ataaagccat tgggtgaaag agtcgttatc aaaatgcttg aaaccgaaga agttaccaag
     3001 ggcggcgtcg tacttcccgg cagtgccaag gaaaggccgc aggttgccga agtcgtggca
     3061 gtcggacccg gcggctatga cgacaagggc agagagatca agatggaagt gaaagtcggc
     3121 gacaaggtac ttatcagcaa atatgcagga accgaagtaa agatcgacga cgtggagtac
     3181 acgatcctca aacagagtga tatcctggcg aaagtggaat gatccccgga tcgtttgttt
     3241 ggttcaataa attattagcg gacaaggagg cataaaacat atggcaaagg aaatcaaatt
     3301 ctcggaagat gccaggcgcg cgctggaaag aggcgtaaac cagctggctg atacggttaa
     3361 ggtaactctc ggaccgaagg gcaggaacgt agtcctcgac aagaaattcg ggacccctgt
     3421 tgttaccaat gacggtgtta caattgctaa ggaaatagaa cttgaagaca aattcgaaaa
     3481 catgggcgct cagctcgtca aggaagtagc aacaaagacc aacgacgttg caggtgacgg
     3541 tacgaccaca gcgaccctgc ttgcccaggc tataatcagg gaaggcatga agaacgtggc
     3601 tgccggcgca aacccgatga tactcaaaaa aggtcttgca agggcagtag aggtggcagt
     3661 tgatgctata aaggaaaaca gcaggaagat ccagggcaag gaagacatag caagagtcgc
     3721 tgctatatct gcaaacgacg aatacatcgg cgaattgatc tcggacgcca tggagaaggt
     3781 cacaaatgac ggcgtcatca ctgtcgagga agccaagacc atgggtacga gccttgaaat
     3841 agtcgaaggt atgcagttcg acaggggata tatttccgca tacatggtaa ccgatactga
     3901 caagatggaa gctatcctcg atgatccgta catcctcatt accgacaaga agctcagcaa
     3961 cgtacaggat ctcctgccgc tcctcgagca gatagtacag gcaggcaaga aactgctgat
     4021 aatcgcggaa gacgttgaag gtgaagcgct gacgaccctg gtgctcaaca agctccgcgg
     4081 cacattcatc tctgttgctg tcaaagctcc cggctttggc gacagaagga aggaaatgct
     4141 cagagatata gctatcctga ccggcggcca ggtgatttcc gacgagcttg gactggatct
     4201 gaaggaagtc aagctttctc agcttggcag ggcaagacag gtcaaggtcc agaaggaaaa
     4261 caccattatc gtcgacggcg caggcaagcc ggaagagatc aagaagagga tcagctcgat
     4321 caggaaccag atcgaagaga ccacttccga tttcgacaag gagaaactcc aggagagact
     4381 cgcgaaactg gcaggcggcg tagccgttat acaggtcggc gctgcaacgg aagtggaaat
     4441 gaaggaaaag aagctccgca tcgaggatgc tcttgccgca acgaaggctg cagtggaaga
     4501 aggtatcgtt gcaggcggcg gtacggcata tgtaaacgct ataccgaagg ttgcaaagct
     4561 gcttaatgaa gtccatggag acgaaaagac tggcgtacag atcatactga aagcccttga
     4621 ggagcctgta aggcagatcg cagccaatgc cggactcgaa ggctccgtca tcgtcgataa
     4681 ggtcaagaac agcccggtcg gcgtaggctt cgatgtactg aaggaagagt acgtcgatat
     4741 gatcaaggca ggtatcgtcg atcctaccaa ggtcacaagg actgccctcc agaatgccgc
     4801 atccatagcc tctatggtgc tcaccaccga gagcgtggta gcagacaaac cggagaaaga
     4861 gcctccgatg cctgcaggcg gcggcggaat gggcggaatg tactaaaaac aggctgcccg
     4921 cggaatatgc ggcatgaatt gacaacagaa cggtttacag gcctgaaagg ggtgccccgg
     4981 tgtagaacca ccaggacacc ccctttttat atgagcgtac aaatgctttt cttgctgtat
     5041 attaaggccg cgattactgc agatgccgcg tcagaccgcg taatctgtgt ccggcacaga
     5101 cccctcgatc ccggacccgg actgccgcgc ggatcggcgt gatctttcat gcagcccggt
     5161 catcctgtgt ccggaggttt gtttacagac aattctttct gatctgtggt atatttgaaa
     5221 ggaaaagcct gtacggcgaa tccagcggac gggaaaggga aagcgatgga cgatcggatt
     5281 ttttacgtag cagccggtat catagctgtt tcagcaataa tcatatccgg gtacgccaaa
     5341 aacaagcgca gggtaaggaa actggagcag gaactcgtag ggcagtgggg cagggcccct
     5401 gagggtaagt acaaggacac cgacatgaaa gccatagccg gcttcttcag ggaattgagc
     5461 cgcaacagcg gcggagattt cctcgttgat gatattacgt ggaatgacct ggacatggac
     5521 aggattttta tgaggatcaa caacacgcag tccactgttg gcgaggagat cctgtattcc
     5581 ctgttgagaa ggccggtatt tgacggatcc gaactcatgg agcggggccg tctcattgag
     5641 tatttcagga cgaatcccga tgagtgcctg gaactccgga agatactggc cgcgctgggg
     5701 aagaaaaggc atgcgcaggt ttacgaatat ttcttcggcg agcctgtcac gttcagatac
     5761 gggagatatg tcctgcaggc agcggcgctg ctcttttcgc cgcttctcat ggccatcgat
     5821 gtttccttcg ggtttgcggc aattgtcgga ttgttcatat ttaacatgct ggcttaccac
     5881 aaggcaaaaa gcgagtacga gatctacctg gccggtctct gttacctggt cgatatgata
     5941 aggtgctcga aaaagatcgc cgcagccgaa atccccggta taaaggaata tgccgccagg
     6001 atggggaacc tgtcggggga aatgagatcc ttcagcataa attctttcta ccagcttttt
     6061 taccagaccg gcgattacat ggttttcgag ccgctcaagt cgatgttcct cctggagctt
     6121 atcgctttcg ggaggctcct taagaccata tatgcccata aacaggtgct ccgcgaaatg
     6181 tatgagacca tagggtacct cgacagcatg gtatctgccg cttcatacag ggaatcgctg
     6241 gacttctgga ccgtacccgc gctttatact agtgacgggg caaagccggt gtcgctttct
     6301 atcagggacg cctgccatcc cctgatagaa aatgctgttg cgaactccat tgatgccgtg
     6361 agaccggtac tgatcaccgg ctcgaatgca tccggcaagt cgactttttt gaagaccgcc
     6421 gccataaatg ccatcctggc acagaccata tacacctgtg tcgcccgcgg ataccgttcc
     6481 tgctatttta aagttttcac gtcaatggcg ttattatata gccgagatca actcgctgaa
     6541 aaggatactg gacaacctgg gaggaccggt accgctgctg tgcgtcattg acgaggtgct
     6601 gagaggaacg aacaccatcg agaggatagc cgcttccacc gagatacttc ggcatttcgc
     6661 cggcgggaac tgtatcctta ttgctgcaac tcatgatatc gagcttgcgg agatactgaa
     6721 aaacctgtat gacaattatc atttcactga gaaatacgaa ggcgatgaaa taaagttcga
     6781 atataagatg catcccggga aatcgacgac gcataatgcg ataaaactgc tcaaagtcat
     6841 cggctatccg gagcatattg tgaaaaatgc ggaagacagg gctgaaaagt tccttgcaga
     6901 gggcagatgg cataccgtta atgcagagtg acagtataag gaggaggagc agaaatagac
     6961 agcgggatca ggaacggcag caaaatcgaa attgaaagca aaaccggcag gagaacaaaa
     7021 aagaaaattc gggttatggc catcatagcc gcaggtcttt tcatcctgac catatttgct
     7081 gctttctatg acggcctgtc agtcaggaga tacaggatag gcaccggcaa actggagcgc
     7141 gggcagaacg tgaggatagt gctgatcgcc gacctgcaca gcagtatata cggagaaaac
     7201 caggaacacc ttgtaaacct ggtaaagagg caaaatccgg atctgatagc tcttgcaggg
     7261 gacatagccg acgacgatgc ccctcatgcc ggaacagaga tgttccttgc cggcatcagg
     7321 gacgcagctc ccgttttcta tgtttccggg aaccatgaat gctggtcccg tgagatcggg
     7381 gagatcaaag atatgataag gggatacggc gtaaccgtgc tggagcacag ctgcaccacg
     7441 atcgcggtca atggcgcgag gatcatcatc tgcggcgtcg acgaccctga catcagactc
     7501 gaaaaaggta cgggctatga ctggaaacag gagatgcatg aggcattcga cagtcttgcg
     7561 gatgagcccg gatataagat actgctgtcc caccgtccgg aacgcgtaaa cacatacacg
     7621 gaattcagct ttgacctggt actgtccgga cattcccacg gagggcaggt caggattcca
     7681 tttattctga acggattgta cgcgccggac cagggatggt tccccaagta cgcagggggc
     7741 gtgtaccgcc acggaagcat gacacatgtg gtaagcaggg gagtttcata taatcccggg
     7801 ctcccaagga tattcaaccc tcctgaggtc gtggtcgtcg acatatacgg caaatgaccg
     7861 gcacaatact gaaatattgt taatcgatga atggtatgat acaatcagaa ttacctgtta
     7921 cactaatttg gatgccaggc gggaggattt cggatggata cttttatgga gaagctcgtg
     7981 accaggaaga aaactcttac tgatcacctt atcacgatag gggccgtcgt cggatcggca
     8041 ttgctgatac tgctggccct ttcgataccg atactgatac agctcgggct gagcctggtg
     8101 ctggcggccg ctgcagcata ccttgggtac agagtgataa cttcgcgtaa tatcgaatat
     8161 gaatatatag tgaccaacgg cgacctggat atcgacatga tagtatccaa aaggaagaga
     8221 aaaaggatat tcagcgccaa ctgcaaggaa tttgacatag tatccccggt aaagagcagc
     8281 catttcgacc agtcggtgca gagcataaaa aacaggatcg atgcttcaag ttccatcgat
     8341 tcgccggatg cgtacttcat cacgctcaat tataacggag aaaggacttt ggtcattttc
     8401 gagcccactg aaaagatgct gaataatttc aggctctata taccgaggaa agtattcaga
     8461 agctgaggtg gtccaatgcc tttcgaactg gaaatattgg caaggctgct gcttgcggtg
     8521 ctgcttggcg gattcatagg ctacgaacgg gaacacacca acaggcccgc cggcttcagg
     8581 acgcatatac tcgtgtgtgc gggagcagca ctggtcatgg ctacttcaga atacatggtc
     8641 aggaggtatt ccatttcagc cgtggaccct gcgagactgg gtgcccaggt cataagcggc
     8701 atcggtttcc ttggcgccgg cactatcata cgcgatggcg tcaatgtcag gggccttacg
     8761 acggcagcta gcctgtgggc agtatcatgt gtcggtatag cggtcggatg cggcttctac
     8821 ctgggagccg tgatagcgac gatattgata ttcataacgc ttatcacgct taaaaaggcg
     8881 gaaaggcgtt tttcgaggcg gaacaggttc aggacgatca tagtcgaatc cgagaatatc
     8941 agcggccagg taggacttgt gaccaatctt ctcgaaaaaa acagcatcga gatcaagaat
     9001 atccagttat ataaaagcaa ggacagcgcc cagatgataa agctgctcgt aaagctgccg
     9061 ggaggcgcca tggatgtgca gacgctgaat gaactgcagg ctatcgaagg cgtcagggaa
     9121 gtatacgagg aatagaagta tgcgaggtat aagtatactg taaacagaat gagtataccg
     9181 ggaacaacgc atgtacgaat gctcacggaa ttatccatat ccgggcggtt ccctgatgat
     9241 catatcttca agatttccgt atgatttctg cacggtttgg gacaacttat tgacacatat
     9301 gacaggacaa ccctgtagtt tgccactttt ccgcatgaag cggcatggtc taatccatgc
     9361 cgcttttgaa tagaaatagg acatagacca aactaatccg gcggaagaaa aggggtgtct
     9421 tacttgcgcg ggcagaatac tcggaggatg atgcgtgaag atgcggaggc agctttgggc
     9481 cttagcagca gggaggctga acggaggctt ctggagtacg gcccgaacct gctgaccgaa
     9541 aagaagaaag tttcgcctgt aaagttgatg ctggaccagt ttaccgacat aatggtcata
     9601 atactcatgg tttccaccgt aatttccgca tttatgggcg aaataaccga agccgtcacg
     9661 attatcgcga tcgtcaccct caatgcggtc cttggcttca tacaggagta cagaacggaa
     9721 aaaacgatgg aagcccttaa gagccttact gcgcctgagg caaaagtcat cagggacggc
     9781 aggcaggtca gtgtgcctgc cgaccgggtg gtcccgggag atataatcgt gctcgaggcc
     9841 ggcgacagga tagctgcaga cgcagttttg ctggacagtg taaatatcca ggccgatgaa
     9901 tcgctgctga caggcgaatc cgtccctgtg gaaaagactg ccgtatcacc cggaaaagat
     9961 cccgccaggg caggcaggaa cagcagggta ttcatgggta cgagcgtaac ggccggccgg
    10021 gccaaagcgc ttgtgactgc cacgggcatg aacactgaaa tgggccgcat cgcggatatg
    10081 atacagaata tagaagaaga acagacgccg ctgcagaaaa ggctcgacaa gctgggcaag
    10141 atcatcgctg caggatgcct tgccatatgt gctgttgtcg ccggcgtcgg tataatacgc
    10201 ggcgaagacg ttttcaacat gctcctttca gggatcagtc ttgcagtcgc ggcagtgcct
    10261 gaagggctcc ctgcgcttgt gacgatctca ctggcgctgg gcgtccagag gatgatgcgg
    10321 aggaatgcgc tcatcaggaa gctcccggcg gtcgagaccc tcggatgcgc aggcgtagta
    10381 tgttcagaca aaacaggcac gctgacgcag aacaggatga cagtcaggca gatctatgcc
    10441 gacggcagga tcatcggggt ggcggatgag aaaaaagtcg gaagacgttt tgataaaaac
    10501 ccggaagggt atttccacgt aaacggcagg gtgctggatc cgacagacag ggccctcagg
    10561 agggtcctgg aaatagctgc gctctgcaat aacgcggtcc cggccggaga aaccggcttg
    10621 aaactgccct cgccggacgt accggcgagc aggtccggac atgcggacaa aatgcggaag
    10681 ttcgcaggag atccgactga aacggcgctg atggctgctg ctgagaaagc agggctgtcc
    10741 tttgaggaac ttggcatgat ctaccgaagg acagacgaac ttcccttcga tccggaaagg
    10801 aaatgcatgt cggtggtctg cgtggaccgt aacggcgaag ctttcctgtt cacgaaaggc
    10861 gcacccgaca tgctgctgaa aaagtgcagc agggtggaga gagccgggac ggtgaagacg
    10921 atggaccata tggacagatc cgggatactg aaggcaaacg accgtatggc ggcaggagcg
    10981 ctgagggtga tagccgtcgc atacaaaagg cttggaaagg gcaggtattc aaggaacggc
    11041 ctcgaaacag acctcatttt cgcaggactg ttgggtatga tcgacccgcc caggcctgaa
    11101 gcggctgaag ccgttatgaa atgcaagatg gcgggcatca ggcctgttat gataaccgga
    11161 gaccacaagc tgaccgcagt ggcgatcgcg aaagaacttg acatatacgg ggagggtgac
    11221 actgtcctta caggcgaaga actggactcg atggacgagg aatcgcttga gaaggcggcg
    11281 gcaaccacca ctgtctatgc aagggtatcc ccgaaacaca agcttgcaat cgtaagagcc
    11341 ctcaaaaggc tcggaagcat cgtagcaatg accggggacg gcgtgaacga cgctcccgcc
    11401 ctgaaacgca gcgacattgg tgtggcgatg nnnnnnnnnn cccgccgtca aggaggccga
    11461 tatcggcgtc gctatgggca ggaccggcac cgatgtcaca aaggaagcgt cgtcgatggt
    11521 gctgatggat gataatttcg ccaccattgt tgccgctata gaagagggca gggtgatcta
    11581 cagcaatata aggaagttta taagatacat gctttcctgc aacctcggag aggtgctgac
    11641 catgttcctc ggcatgctgg caggccttcc ggtgcccctg ctgcccatac agatactgtg
    11701 ggtcaatctt gtgacggacg ggctgccggc tgtggcgctc ggtttcgacc cgcccgaaaa
    11761 ggatatcatg aaacagagac ccagggatcc cgaagagagt attttttcac aaggccttgc
    11821 agggctgatc attgtgagag ggatcctcat cggcctggcg accctggcgg tatacgcttc
    11881 gcttctttgg tatacggctg atatagggct tgccaggacc ggaggttttg tcacgctggt
    11941 actgtcgcag ctcatacatg tattcgaatg taaatccgaa acgcggaaca ttttccggat
    12001 accgctgttc aacaacattt tccttgtact tgccgtgatc tgctcatcgg tcatgatgct
    12061 cggcgtcata tatatccctg tcctgcagaa catcttcaaa acagttccgc tggggctgaa
    12121 cgagtggatg atcgcagggg gactttcctt cctggctccg gtcctgacca gcatgttcat
    12181 gccgcgccgc taaataaaaa aaggggacat tcctcgcaaa acaaggtatg gtgacactca
    12241 tctgcaaaga agtattcctc tatggctgag gaatgtcacg catgcctgct gaggagtgtc
    12301 cccaaacctt ttacatgggg acattcctcg cagaaaaagg tatggtgaca ctcctctgcg
    12361 aagaagtatt cctctatggt agaggaatgt cacgcatgcc ttgttgagga gtgtcccctt
    12421 acccttttcc tgataaaata ttccttgaaa tgacattata aaaaatatat aattagtgga
    12481 gcctgaaatg tccttaatga aagcattttt ggctatcata aattgcatgc gctttttaac
    12541 agattgttca caatttatta aaatgtatct ctcataattg tattggcgca gtgtgtcaag
    12601 agacgtttgt tgcaggaggg gcaaatatga tagaaataca gaacctgacc aaacgatacg
    12661 gtcagataac agctgtgaaa gacctgaatt tcaccgtcga gaaaggtgaa atacttgggt
    12721 tcctcggtcc gaacggagcg ggaaaatcca caacgatgaa cataatcaca ggctacatac
    12781 cttcgaccga agggactgta aaggtggatg gatacgacat cctggaatcg ccgaaggaag
    12841 tgaagaggcg cataggtttt ctgccggaac agccgcctgt ctacatggac atgactgtcg
    12901 cagattacct ggattttgta gccgatctga aaaaggtgga caagaagcaa aagagaagcc
    12961 agataagtga cataatggaa cttgtcaaaa taggggacca caggaacagg ctcataaaga
    13021 acctgtcgaa agggtacaaa cagagagtcg gccttgcgca ggcccttgtc ggaagccccg
    13081 acgtactcat cctcgacgaa cccaccgtcg gtctcgaccc caagcagatc atcgagataa
    13141 ggaagcttat aaaggctctt ggcaaacagc atacaatcat cctcagttcg catatcctgc
    13201 ctgaggtcag tgccgtctgt gaaagggtag tcattatcaa taaaggcgag atcgcggcca
    13261 tcgatacgcc tgaaaacctg tccaggggtc ttggctcagc ttcgaagctg acggctacga
    13321 tagcaggtcc caggagttct gtggagaacg ccatcaggga aatatacggc gtcaaatatg
    13381 ttgagtctgc agcagacaag ggaaaagagg caattagcta catcatcgaa tccgacaagg
    13441 atgtggacat caggaagcct ctgttctttg cgatggcaaa gctgggttat ccgataatcg
    13501 agctcaagag tctcagcctg agcctggaag acatattcct gcagatcgtg acagaggaaa
    13561 aggaggtcgg ttgacgtatg gcagcgatat tctggaaaga agtcaaatcc tatttttatt
    13621 cccctatggc ttatatactc ataggtttgt tcatcctcct gacatcgata atgttccttc
    13681 catatctcag atatggttac ggtcttttca acgatgtcct cgggaccatg ggattcatac
    13741 tcctggtcgt agtgccgatc ctgaccatga ggatcctggc tgaagaccgc aaaaacggca
    13801 ctgaggtgct tctgatcaca tcgcctgtga atatcacctc gatagtcgta ggaaaattcc
    13861 ttgcggttta ttttgttttc cttgtaatga caacgcttac attcgtatat ccgatagtgc
    13921 agctggcatt cggagccaag ttcactgtac agctcatagg aggctatatc gggttccttc
    13981 tcctcggcgc gacttatatc gctgtcggcg tttttgcttc atcgctgact gagaaccagg
    14041 tcatagctgt gatcatcagt tatgtatcac tcctgataat gtggctggca gacagcgtga
    14101 gcagcctggt gggcggtttt gttgcaaaag tgctcaactg gttctcgatc ctgtcgagat
    14161 acgacgactt caacagaggc atcctgggac tcagccctgt tgtttactat ttgagcttta
    14221 ccgcggtatt tctgttcctt acggtaagag tcatcgaaaa aagacgctgg agtcaggggt
    14281 gatatgaatg gctaaaatcg ataagaaaaa gatcaaaaac tttttaaata aaaagagttt
    14341 gaaatacggc acgaacgcag ccgtcataac tgccgcggtc atagtcatgg caatcgtgct
    14401 gaaccttctc gtagggctgc tggacctgaa actggacctg acgcccaaca aactgttttc
    14461 tctttccgag gccacggtga acatactgaa cgaactggat caggacgttg agatcatcgg
    14521 cctgttcgac gacggcgcaa tttcgtccaa cgacgagtac aagcaggtga cggatctgct
    14581 gaacctgtat gcgaagtacc cgcatataac ggtcaggtat gtggatcccg acaggaatcc
    14641 cggcattatc agccaactgg accctgacga cacgctcgac ctggggtcga cagacttcct
    14701 cgtaaggagc acggtcaacg gcgccgaaaa gaaaaagaaa cttgaatatt atgacctttt
    14761 tgaattggaa tacgaccagt attcgatcag ttcgtacatc accggttcca atgcggagca
    14821 gggtttcacc ggcgcgatca aatacgtgac gtccgaatat actcctgtag tctacttcac
    14881 tgaaggacat gacgagtatg atgtggatta ttattacagg actctgaagg gatacctgga
    14941 aaagaacaat atactcgtga aaaagatcaa cctgctgacg gtcgaccaaa tgccggaaga
    15001 tgcggaactt gttgtcgtcg cttcgccgaa gaaagacttc cagtacgccg agagggatgt
    15061 cctggaaagg tatttcaacg tcggaggaaa agcgatcttc atgttcgact atctcaagaa
    15121 cgacccctct ttcgatgaac tgaacaagct gcttaacaag tacaaccttg aaatcaatta
    15181 tgacaaggtg aaggagactg atgacagcag gcacctggcc caggatccgt atacgctggt
    15241 ggtggatgtg aaatccaatg ggatcgtatc cagggcattc aatactttcc tgaccaactg
    15301 caggagcata agcatactga agaatgaaaa ggaatatata accacgactt ccctgatcag
    15361 cacgagtccg accgctgtgg gcgaaatggt caacaaatcc cgcggagacg acctgccagg
    15421 tcccctcgaa cttgcggtgg cggttgagta caagggcgga gagaagccgt cgagaataat
    15481 cgcaatgggc aactcttcat tcgttaccga cagtgcttac caggtatacg gcgacttcta
    15541 cttcagcaac atcaacttct tcctgtcggc agtgaactgg atgatcgaga taaaggacga
    15601 agtaatagtg cccaccaaga gctatgatgt gaacaggttc aatataaccg ggcaacaggc
    15661 gacagtgatg acatggatcc tggtggtagt attcccgctt ctgatacttg gcacaggtct
    15721 gatggtatat ctgaggaggc gtcatttatg aaattttaca ggaatgcgat cattctcgta
    15781 gtcgtagttg cgcttcttgc cggtgcgtat ttcctggtca ggaccaaaaa ggccaatcaa
    15841 cagtcggatg tagccaaaaa atatgataag ctgacggatt atatttccac cgacatagag
    15901 tctgttacat tgaaaaacag cgacggcacc ttcgtgatcg tcaagaagga cgatgaatgg
    15961 gcgctgtcca ctcccacgga cctcaggtac gattcgtcgc agctcagcag tatagtcatc
    16021 aattcagctg caatcgtggc agacaaaatg gtggaagaga atgcggagga cctttccata
    16081 tacgggctcg acgatccggc aactgccata ataaaggcaa aagacggtac cagcgtgacg
    16141 ctggagatcg gaaaccagac gcctaccggg ggcggatact acgtaaagcg ggcaggcgaa
    16201 aacaaggtat ataccataag caaatatacc ggggacaagc ttacttcagg aaggaacgac
    16261 ataaggacga aacagctgtt tgacattacg ccggacgaca tacagaagtt cggcctgaac
    16321 aggaaagggc agaacatttt tatttcagag aagatagacg atacatggaa catggtatca
    16381 cccataaagg ggagcatgaa tgatacgtcc ttccttccga tgctggaagc cgtcgccggc
    16441 gccgaggtcg tcgatttcgt cgatgacgat cccgaggatc tctccgtata cggacttgac
    16501 aaaccgacat acgaactgat cttcagcacc gaagccacag gcgagataaa gctgcagatg
    16561 ggtcttgaac tgaaaaacga ttcgaccata tacgcgaaac ttgacgggaa cgacgaggtg
    16621 ttcaccataa gcacaagccc gtttacattc cttgacaaac cgcttaagga gatcgtaaat
    16681 gtctttgcct acatcgtgaa tatccaggat gtcaggcaga tcgatctgac gatggacgga
    16741 aaaacgaccc acatggatct tgacgtatac ctggatgaga acggcaatat ggattcagac
    16801 aaggacaagt tctatgtgaa cggagttgat gccagcggca ggaacgaaaa cggcaagcag
    16861 ccgttcaggc agttctacca ggcgctgatc ggcatcagca tcgatgagat agacgtggac
    16921 ggcgacccgt ccggcgggaa ggccgatata acgatagact atactctcaa ggacgggaag
    16981 atgaaagtgg aattcatacc gaaggatgaa aacttctact acgtggtgag gaacggcgag
    17041 tacgccggcg tgctggtaaa aaggaagaac aagggcgaat tcggcgttga cggaatgaaa
    17101 gaggcgtaca ataccatgat ggaattcctt gccggacagc aggaataacg cagccaggta
    17161 cagcacgata aatatataat gtaatttggt aaatagctcc gacatagaat gaactggtac
    17221 attctttgat cggagctgtt tttatggatg acgagcgcag gaacatactg agggtcgcag
    17281 gtatatacgc ggcaagcata acgggggcgg gattcgcgtc agggcaggag attatgcagt
    17341 ttttctccgt ttacaaaacc ggaggctttt gcggtatatt gcttgcaggc tcgctgttca
    17401 gcgttttggg ctgtatcgtc ctcggcaagg t
//
LOCUS       DAOSCC010000018        17414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2258_length_17414_cov_4.666340, whole genome shotgun sequence.
ACCESSION   DAOSCC010000018 DAOSCC010000000
VERSION     DAOSCC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_17414_cov_4.666340"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLG72_02020"
     CDS             <1..985
                     /locus_tag="PLG72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ79612.1"
                     /translation="DDIQSHFGDWTFLSHPQPKPHEAPKLPVHTILGGVRELAEKRGI
                     KVVYHKGCDVMDMDDEDISGAVECASGSDVIIAVVGDCLAQNGEFKDRADLSLSGNQQ
                     KLLESLKECGKPLIVVLVNGKPLAIPWVAENADAVLEAFNPGLFGGKAVAEIIFGELN
                     PNGKLPISFPYHSGQLPVYYNQLPGWHGGRYMDMPCKPLYSFGYGLSYSQYEYSNLRL
                     SRTRCSANDTVTVSVDVTNRGPYDGKETVQLYVNDVISSVVTPVRQLKGFVKTEIRSG
                     ETKTVEFALPVSELYIIDENENQVVEPGDFEILVGPDSRPESLLKAVLTVV"
     gene            1265..2236
                     /gene="pfkA"
                     /locus_tag="PLG72_02025"
     CDS             1265..2236
                     /gene="pfkA"
                     /locus_tag="PLG72_02025"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ79613.1"
                     /translation="MSDIKTIGVLTSGGDAPGMNAAIRAVVRTGIYHGFKVMGIYRGY
                     NGLINGDMFEMDLRSVSDIIHRGGTILKTVRCQAFKTEEGIQKAISMAQVFGLDAIVV
                     IGGDGSFKGARDLARRGVKVMGIPGTIDNDIGCSEYTIGYDTCLNTVQDALDKIRDTA
                     YSHERCSVLEVMGRHAGYIALNVGIASGAEVVILPERPFDFNKDIIKPILEGRNRGKK
                     HYIVIVAEGVGGAVNLAEKIEEITGIETRATILGHIQRGGSPTVRDRVMASMMGAKAT
                     ELLANGIYNQIIAVKAGEIVGIDIEEALETKKEIPKDMLELSTILSL"
     gene            2459..2890
                     /locus_tag="PLG72_02030"
     CDS             2459..2890
                     /locus_tag="PLG72_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ79614.1"
                     /translation="MEQVELNKMLDNREFLDKLYSFAYVRCSSSHEAEDLCSDILVSI
                     MASAQKSPEIHNLNAFVWTIAHRVYADHCRSRKKERSVQITGNFSDMVFAITDDPIDH
                     VLELVFQPVINYAIEKCIENGLLNIPETSPCAEGTWMVVSK"
     gene            2947..3963
                     /locus_tag="PLG72_02035"
     CDS             2947..3963
                     /locus_tag="PLG72_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79615.1"
                     /translation="MLSRKEFTEMAMRLYEFCEYPAVRYKILYHLLDRPYDDPELSSL
                     RPAFLNSDIVEELWREQDEYGGWGPLRDKDYSVKARFPTSLVAIDRCLYIGLTIDDRD
                     ILFNAYEYLQDFLRGTSREKLYDKNERAIPWQIAVICETIESIRPYNELCDRTYSEWM
                     YIVSRAYEDGEYSYERERDAQHEVFGTREKRLVPMQFGLLLKRRENVPPKLEHAMLLH
                     HGRHAYYHGYFWENCPAKLPDNFVYEKTRRWMYSFNYINQFRGSALFLADAVEWLLSC
                     RNADGLWDWGPQVKDPWGYFRYFSTNRHYQHNRIVDCTMEILDFLKKYIDNNTGSNVE
                     PVSG"
     gene            complement(4089..5171)
                     /locus_tag="PLG72_02040"
     CDS             complement(4089..5171)
                     /locus_tag="PLG72_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOJ79616.1"
                     /translation="MGRKSAKPGKIRAFLERKGIVISGRLYAIDAMSAMAQGLFCSLL
                     IGTIINTIGTQTGLAFLNKIGKFASDMSGPAMAVAIGYALKCPPLVLFSLAAVGYSAN
                     QLGGAGGPLAVLIIAIIAAEIGKAVSKETKVDILVTPLVTIFTGVGVSALIAPPIGAA
                     ASSIGEVIMWATEQQPFIMGILVSVIVGIVLTLPISSAAICHMLGLTGLAGGAALAGC
                     CANMVGFAVISFRENRWGGLVSQGLGTSMLQMGNIVRNPKIWIPAILASAITGPISTV
                     VFRLEMNGPAVASGMGTCGLVGPIGVYTGWINDIAAGTKTAITAFDWFALAMISIVLP
                     AVLSYIFGLICRKIGWIKEGDLKLDN"
     gene            complement(5346..6728)
                     /locus_tag="PLG72_02045"
     CDS             complement(5346..6728)
                     /locus_tag="PLG72_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ79617.1"
                     /translation="MDISKHHDMDMCNGPLFGKILIFALPIMAMNILQLLFNAADMII
                     VGQFSGKEALAAVGATGALINLLVNTFMGLSVGTSVIVAQDYGAGHPEEVSRSVHTSI
                     AVSIISGVIVMAAGLVLCKPMLAWMGTPEDIIDLSVLYMRIYFLGVPANMVYNFAAAI
                     LRSVGDSRHPTYYLFISGVANVFLNMFFVIAVGMSVDGVAWATIITQYLSAFLTLACL
                     YRSSLSIRFIPRRLRIDGQKLKILLRVGIPAGLQSALFSISNTLIQSAVNSFGSTMVA
                     ANSAASNVEGLAGTTMNAYYNAAVTFTGQNMGAKKYDRIDTIAKICAALVFATWLVLG
                     GAILLFGRPLLSLYTTEPEVIELGMERMYIMMAAYFTCGIMNVFPGLTRGMGYSILPM
                     LCTLIGACLMRIVWLATFFTWYRSIFMLFICYPITWALAGFGQVASFFYARHRIRKRA
                     ATENGAASAG"
     gene            complement(7024..7557)
                     /locus_tag="PLG72_02050"
     CDS             complement(7024..7557)
                     /locus_tag="PLG72_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ79618.1"
                     /translation="MDLKGTKTEKNLWEAFAGESQARNKYTYFASVARKEGYEQIAAI
                     FEETANNEKEHAKLWFKALGELGGTAENLLSAANGENYEWTDMYERFAKDAEEEGFTV
                     LAKQFRMVAAIEKAHEERYRKLLSNVEMQQVFEKSEMTIWECRNCGHLVMGKKAPEVC
                     PVCGHPKSFFEVRKQNY"
     gene            complement(7586..7942)
                     /locus_tag="PLG72_02055"
     CDS             complement(7586..7942)
                     /locus_tag="PLG72_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ79619.1"
                     /translation="MTRQRKTILNIIKTSNEHMTAEEIYIKAKKEMPSIAIGTVYRNL
                     GLMTDAGEIRRIPIPNSPDRYDKSTIPHEHLVCQKCGTVHDMFIEGLREFLQRQTGIE
                     ITGYDLNLKFICENCK"
     gene            complement(8096..8863)
                     /locus_tag="PLG72_02060"
     CDS             complement(8096..8863)
                     /locus_tag="PLG72_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79620.1"
                     /translation="MRRKDIYTDEELEANKMAAGKLMMVFIIILLITVMAAGCVPIKY
                     SGNVKTDTHVGGSTDMDVRQDDGSASATVQPGGSGDVAVEGPSETGAAEISPLTDEEV
                     AGAYQKAVEAFGWFEFGTMPADYEDVKEADGYRYYRVTHDTIKTYEDLRSYLQAMFTD
                     EIVERLLAGDGSGILLYRDFDGSLYTISAGRGSDITKGEETYEMIREGDKKVIYRVTV
                     EVCDDPFEETVAGTEQYDFPLEYIDGRWVFTSFRLVR"
     gene            complement(9193..10248)
                     /locus_tag="PLG72_02065"
     CDS             complement(9193..10248)
                     /locus_tag="PLG72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ79621.1"
                     /translation="MQFGIRLNSKDISIRSSLILVLSYAMAAIIAFVVYTAGGTSKVY
                     AHLMHIPITIVSSTNGKKHGIINAIAGGLLLGPFMPMDAEHNIMQPPVNWIIRTVIYV
                     IIAYVIGFFADHYRQELTNVRKRDDEIFEAHMATIYSLVKLAESRDYNTGEHVLRVAE
                     LCRLLADKLRSREKYRGYINDSYIEYIFKAAPLHDIGKVGIPDSILLKPGKLTPEEFE
                     IMKTHTIIGEETLLEVKQRYSGYKFLEMAVNIARHHHEKWDGTGYPDGLSGDKIPLSA
                     RIMAVADTYDALRSKRVYKDALSHAESLEVIRQGAGTHYDPEIVEVLLEYEDEINEIY
                     KKAGPGNPETVPLEIRE"
     gene            complement(10399..11217)
                     /locus_tag="PLG72_02070"
     CDS             complement(10399..11217)
                     /locus_tag="PLG72_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79622.1"
                     /translation="MPNSKKYLEWVVLMSAIFVIVLGIGVAGGRAVPAETPGTEEQFE
                     ISEDGDIPGMPGGESGTDTLITGRDPGIAGKDDMELVAVADLDRDGDEDEICIDKSLM
                     EGTNYITLRIIDSKGNEIWSESANTAHAGWNSLFLCEHEGGYYLLRYNPTMYQGYATY
                     AYTLFTLEDGRQKVYRTNSLEFDINGTCELDVPKMIAFADDVNAYLGKSTLLMSTEGG
                     SLCLGPSPAEPFLERYSWLDAFPYVYEADDDLGTRLAKYSEYAVSNRDNTGDRT"
     gene            complement(11348..12127)
                     /locus_tag="PLG72_02075"
     CDS             complement(11348..12127)
                     /locus_tag="PLG72_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ79623.1"
                     /translation="MSELRIAAQLYTVREFMKTPKDTVNTLKRVKEIGYDAVQVSGTG
                     YIDREKAELVKKAAEELGLAICATHVGFDQLEREMDWMIELHKMWNCRYVGLGAMPER
                     FRGSREEYVEFAGIANGFGKVLSRNGLRFIYHNHNFEFHKFDGMTGMQILLTETDPEY
                     FGFEIDTYWVQAGGADPIEWIRKLKGRVDVIHFKDMVMHQGKQTMAEVGEGNLNWNGI
                     IDACRETGVKWAAVEQDICLRDPFESLGISLRNLEEYGCMS"
     gene            complement(12257..13288)
                     /locus_tag="PLG72_02080"
     CDS             complement(12257..13288)
                     /locus_tag="PLG72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ79624.1"
                     /translation="MSKIGVGIIGCGSIFGVHADAVKKSWIAELKAVADIKESAAKEA
                     AIRHGCDFYTDYRKMLKDPGIQAVHICTPHYLHASMAIDALKAGKHVLTEKPVAVNVE
                     DAAKMAETARACGKHLAVCFQNRFNPTSVKAKEVIDSGTLGKVKGIKGIVTWCRNEAY
                     YTESGWRGDREKAGGGVLINQALHTLDLMQWFGGEVERISGHADTRCFRGMIDVEDTA
                     EASIWFKNGARGILYATVCFTDNSPVIIEIHCEKGSLLIWDGELYIARDGKRELLASD
                     QPAYGEKAYWGLSHEKLIRRFYECLMYDWTDYISVCDAAVSVEMVRGIYESSVTGREY
                     RLKGCRMMK"
     gene            complement(13493..14650)
                     /locus_tag="PLG72_02085"
     CDS             complement(13493..14650)
                     /locus_tag="PLG72_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ79625.1"
                     /translation="MDKVRVGIIGIGNMGSSHAMNLAAGKVPDARLAAVCDLRPERLE
                     WAKKALGEDVQRFSTPDELFRAKAIDAVMVCTPHYDHPPLAVRAFENGLHVLVEKPAG
                     VYTKQVREMNEAAAQSGKVFGIMYNQRTNPLYQKARELVKSGELGDIRRTNWIITSWY
                     RSQSYYDSGGWRATWSGEGGGVLLNQAPHQLDLWQWICGMPVRVRAFMSFGKMHDIEV
                     EDDVTAYVEYENGATGVFITSTCDTPGTNRLEITGDRGKIVIEDGKIVFHRLRVSERQ
                     FNKEYKGGFGEPECWKCEIPVNGHQTDHVGIMNNWIDAITKGTPLLAPGEEGILGLTI
                     SNAMHLSAWTDGWVELPIDEDLFYDKLQERVKTSTFKKDAKDVTLDVSGTY"
     gene            complement(14650..15516)
                     /locus_tag="PLG72_02090"
     CDS             complement(14650..15516)
                     /locus_tag="PLG72_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ79626.1"
                     /translation="MSRFILSAFADEIDPGLDVQMDVLEQHGISYIEMRGVNGKNITQ
                     HSPEEVKGIKKQLDKRGFRISAVGSPIGKINITDDFGPHLELFRHTLEIAKILETRYI
                     RMFSFYIPEGEKPETYRDEVMDRWRRFLDAAEGTGITLLHENEKGIYGDTPQRCLDLV
                     KEMDSENLKLIFDPANFVQCDVETYPEAYELLKEHVVYLHIKDAVYSDHHVVPAGQGD
                     GKVKEILESLYKRGFDGFLSIEPHLWNFVGFAELENRKAETSAEDSGPDKFAIAAYAL
                     KKLIKEITGEAK"
     gene            15732..16571
                     /locus_tag="PLG72_02095"
     CDS             15732..16571
                     /locus_tag="PLG72_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79627.1"
                     /translation="MDPHLYEYSYENQFNISHTADPKAPAEYPVHIHDRYEIYYFLSG
                     DVTYFIEGQSYPLSRHDLLVINTGELHRPVFNSSRPYERITIHFRPEYISSFQQKDYN
                     LLYCFERRKLGHQNRIDGSDIIESQADQYFYKIRKYATERVRESDLMIKTYFLQMLVA
                     LNRIFADKSTTTKNTMAYDVKIVEILDYINNHLHEQITLDSLEEKFYVSKYYLCHLFK
                     KNTGVTVGEYIAYKRILKAKELLIMGAPVLETCHTVGFRDYSNFYKTFRRLEGVSPRE
                     YKQ"
     gene            complement(16605..17129)
                     /locus_tag="PLG72_02100"
     CDS             complement(16605..17129)
                     /locus_tag="PLG72_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ79628.1"
                     /translation="MELIDIYDELGQKCGTEEKYEAHRKALIHKGVCVWIINSNNEIL
                     LQTRNSHAKFPNMLDISFSGHIRTGETSLEAAIREGKEELGIDLDIDKLQYLFTCREY
                     LKADGYFENEIHDVFLYRKDIPVEEYSFSDNEVKEVSYVPLEQFRIMVKTKSAMLMPF
                     KTHYKFLLTALDGG"
BASE COUNT         4212 a         4626 c         3952 g         4624 t
ORIGIN      
        1 cgatgatatc cagtcgcatt tcggcgactg gactttcctg agccatccgc agccgaagcc
       61 gcacgaggcg ccgaaactgc cggtacacac gatcctcggc ggggtcaggg aacttgccga
      121 aaagagaggt atcaaagtcg tataccacaa gggctgcgat gtcatggaca tggatgacga
      181 ggatataagc ggcgctgtag aatgcgcctc cggatcggat gtgatcatag ccgtggtcgg
      241 ggactgtctc gcacagaacg gcgaattcaa ggacagggcg gatctgtcgc tttccgggaa
      301 ccagcagaag ctgctggaga gcctgaagga atgcggaaag ccgctgatag tcgtacttgt
      361 caacggcaaa ccgctggcaa taccctgggt ggcggagaac gccgatgccg tgctggaagc
      421 cttcaaccca ggcctgttcg gaggaaaggc tgtggcggag atcatattcg gcgaactgaa
      481 ccccaacggg aaacttccga tatccttccc ataccattcc ggccagctgc cggtatatta
      541 caaccaactg cccggctggc acggcgggcg gtatatggat atgccctgca aaccgctgta
      601 cagcttcggt tatgggctct cctacagcca gtatgaatac tccaacctgc gcctgtcacg
      661 caccaggtgt tccgccaatg atacggtgac agtatccgta gacgttacca acagaggtcc
      721 ttacgacggc aaagaaactg tgcagcttta tgtgaacgac gtcataagtt ccgtggtcac
      781 gcctgtcagg cagctaaaag gcttcgtcaa gacagagatc aggagcgggg agaccaaaac
      841 ggtggagttc gctcttccgg tcagcgaact ttatataata gatgaaaatg aaaaccaggt
      901 agtggaaccg ggagatttcg agatactcgt aggtccggat tccaggccgg agagcctgct
      961 gaaagcggtc ctgactgttg tatgacaaaa gtgatattct cggaaatgat aattggagac
     1021 acagcaaatg catataacac acttacttaa cagaactgcc atctgttatc cggtgtatct
     1081 ccggattgta caaactgccc ggtaaatccg ggcttttttc tatccctttt tcacttgaca
     1141 gggcatctga ttctgataat ctgtatgtag aaacatatat ggacatgggt cgatccgaca
     1201 gattgtcctc cgatatattg ttctgagtcg taagacagga atgtttgcac agggaggttg
     1261 ttttatgagc gatataaaaa ccataggcgt actgacaagc ggcggagacg cgccgggaat
     1321 gaacgccgcg ataagggcag tggtcaggac aggcatatac cacggcttca aagtcatggg
     1381 tatatataga ggttataacg gtctgataaa cggcgacatg ttcgaaatgg atttaagaag
     1441 cgtttcggat atcatccaca ggggcgggac catcctcaag acagtccgct gccaggcatt
     1501 caaaaccgag gagggcattc agaaagctat ttcgatggca caggtattcg ggctcgatgc
     1561 tatcgtagtg ataggaggag acggctcctt caaaggcgca agggacctgg caaggcgcgg
     1621 tgtcaaggtg atgggcatac ccggtaccat cgataatgac atcgggtgct ccgaatacac
     1681 cataggatac gatacctgcc tcaatacagt ccaggacgcc cttgacaaga taagggacac
     1741 ggcgtattcc catgagcgct gcagcgtcct tgaggtaatg ggaaggcatg cagggtatat
     1801 tgccctgaac gtcggtatag ccagcggcgc tgaagtggtg atactgccgg aaaggccgtt
     1861 tgatttcaac aaggatatca taaaaccgat actcgaaggc aggaacaggg gaaaaaagca
     1921 ttatatcgtg atagttgccg aaggggtcgg cggagcagta aaccttgcgg aaaaaatcga
     1981 agaaatcacc gggatcgaaa caagggcaac aatacttggg cacatacagc gcggcggaag
     2041 ccctacggtg cgcgaccgtg tcatggcaag catgatgggc gccaaagcga cggaattgct
     2101 ggctaatggg atctacaacc agatcatcgc agtaaaagcc ggcgaaattg tcggcataga
     2161 tatagaagaa gcgctggaaa ccaagaagga aataccgaag gacatgctgg aactcagcac
     2221 gatactgtca ctgtaatggc ggaatcttct tcgtggtcat ataattgagt ctgatcagca
     2281 caattatttc agtaacttaa tatgcaaatg ttgttctcct gaatcggtca gcccagttta
     2341 tataccgggc tgatttttat ttcagcagtt ttgttataca atggtgctaa ccggactgat
     2401 tttccaccga ctatatatat gaaatcattc atgaatggtt gagaggcagc agaacggtat
     2461 ggaacaagtc gaactgaata aaatgcttga caacagggag tttcttgaca agctttacag
     2521 cttcgcctat gtgcggtgca gcagttcaca tgaagcagag gacctgtgtt cggatatcct
     2581 ggtatccata atggcgtcgg cccaaaaaag tcccgaaatc cacaatttga acgctttcgt
     2641 gtggacgatt gcgcacaggg tctatgctga ccattgcaga tcacgaaaaa aggaaagatc
     2701 tgtgcaaatt acgggcaact tttcggatat ggtttttgcc ataacggacg atccgataga
     2761 ccatgttctc gagctggttt ttcagcctgt aatcaattat gctatcgaaa aatgcatcga
     2821 aaacggactg ctcaacattc cggaaacctc accttgcgct gaaggtacat ggatggtagt
     2881 gagcaaataa taatgtgtta taatataggc aaatgcaacc aaaagaacat gtcgtgaggt
     2941 ccgctgatgc tgagcaggaa agaattcacg gaaatggcga tgaggctata tgagttctgc
     3001 gaatacccgg cggtgaggta taaaatactt taccacctgc tggacaggcc atatgacgat
     3061 ccggaactca gcagcctgcg ccctgcgttt ctcaacagtg acatagtcga agaactctgg
     3121 cgcgagcagg atgaatacgg cggatggggt cctttgagag ataaggatta ttcggtaaag
     3181 gccaggttcc ccacatcgct ggtcgcgatc gaccgctgcc tgtacatcgg tcttaccatc
     3241 gatgaccgcg atatactttt caacgcttat gaatacctcc aggatttctt aaggggcacc
     3301 agccgtgaaa agctgtatga caagaacgag cgggcgatac catggcagat agccgtcatc
     3361 tgcgaaacga tagaatcgat caggccttac aatgaacttt gcgacaggac atacagcgag
     3421 tggatgtaca tagtgtcccg cgcctacgag gacggcgaat acagctatga acgggagcga
     3481 gacgcccagc atgaagtttt cggaacccgt gaaaaaaggc tcgtgccgat gcagttcggg
     3541 ctgttgctga agcgccgcga aaatgtccct ccgaaactcg agcatgcgat gctcctccac
     3601 cacggaaggc acgcctatta tcacgggtat ttctgggaga actgccccgc gaaactgccc
     3661 gacaatttcg tctatgaaaa gacaaggcgc tggatgtatt cattcaacta tataaaccag
     3721 ttcaggggtt cagcgctgtt cctcgccgac gcagtggaat ggctgctttc atgcaggaat
     3781 gcagacgggc tctgggactg gggtccgcag gtcaaagacc cctgggggta cttcagatac
     3841 ttctccacga accggcacta ccagcataac cgaatagtcg actgtaccat ggagatactg
     3901 gattttctca aaaagtatat agacaataat accggttcga atgtcgaacc tgtttccggt
     3961 tgaccggtat gtatgaacag ggaccgcctt aacggagaac aggccgatcc ccggataacc
     4021 gccgcaataa catgcacatc gcacctgata aaacggccgg ctccaggagc cggccgtcac
     4081 ggttttcatc agttatccag tttgaggtct ccttccttta tccagccgat cttccggcag
     4141 atcaacccga atatataact caaaaccgca ggcagcacga tacttatcat tgccagcgcg
     4201 aaccagtcga acgctgtgat cgcagttttt gtacctgctg ctatgtcatt gatccatccg
     4261 gtatacactc ctatcggacc tacaagaccg caggtaccca taccggacgc taccgccggg
     4321 ccgttcattt ccagcctgaa cacgaccgtt gagatcggtc cggtgatggc acttgccagg
     4381 atggcaggta tccatatctt cgggttccgg actatattcc ccatctggag catcgacgtg
     4441 ccaagtccct gggagaccag tccgccccag cggttttcac ggaaagatat gacggcgaac
     4501 cccaccatgt tggcgcagca ccccgccagg gcagcgcccc cggccagccc ggtcaggcct
     4561 aacatgtggc agatcgccgc gctgctgata ggcagtgtca gcactattcc tactatgacc
     4621 gaaaccaata tgcccatgat gaacggctgc tgctcagttg cccacattat gacctctcct
     4681 atggaacttg ctgccgcacc gatgggaggc gcgataagcg cagaaacacc aaccccggta
     4741 aatatggtca caagcggagt gaccaggata tcgacctttg tttcttttga aaccgccttg
     4801 cctatttcag cggcaataat agctataata agtactgcca gcggacctcc cgcgcctccg
     4861 agctggtttg ccgaatatcc cacggctgca agcgaaaaca ggaccagcgg cgggcatttc
     4921 aaagcgtacc ctatggcaac tgccattgcc gggcccgaca tgtctgaagc aaacttgcct
     4981 attttattca ggaaagccag tcccgtctgg gtaccgatgg tattgattat cgtccctatg
     5041 agcagcgaac agaacaggcc ctgcgccatg gcactcatgg cgtctatggc atagaggcgg
     5101 ccggaaatga ctatcccttt tctttcaaga aatgcacgta tcttacctgg ctttgcactc
     5161 ttccttccca tatgaagcta tcccccatat cctgtatatt tcgggcagaa tccacagcgc
     5221 caaatatagc agaacgttat ctctgcaacc ttaagttatc atgtttagta caattttatc
     5281 cgaaatatcc gatttgtcaa tcatgaagtt tacgatattt cagcccggga tcccaacgtt
     5341 ttcagtcatc ctgccgatgc agccccgttt tccgtcgccg cacgcttccg tatcctgtgg
     5401 cgggcataga agaagctcgc tacctggccg aatcctgcta atgcccaggt gataggatag
     5461 caaatgaaaa gcatgaatat cgagcgatac catgtgaaga aagtggccag ccagacgatc
     5521 ctcataaggc acgcgccgat cagcgtacag agcatcggca gtatagaata gcccattccg
     5581 cgggtcaggc cggggaaaac gttcatgatg ccacatgtga aatatgcagc catcatgatg
     5641 tacattcttt ccatgccaag ctcaatgacc tccggttcag tcgtgtacaa gctgagcagc
     5701 ggccttccga aaagcagtat cgccccgccc agcaccaacc atgtggcaaa gaccagtgca
     5761 gcgcatatct ttgcaatggt gtcgatgcgg tcgtactttt tcgcgcccat attctgtccc
     5821 gtaaatgtaa ccgccgcgtt gtaatatgca ttcatagtag tgcccgcaag gccctccaca
     5881 ttgctggcag cggaattggc cgcgaccatg gtggatccga aagaattgac agccgactgt
     5941 atcagtgtat ttgatatgga aaaaagcgca ctctgcagcc cggcaggtat gcccaccctc
     6001 agtaatattt tgagtttttg cccgtcgatg cgcagcctcc gtggtatgaa acgaatcgac
     6061 aggctgctcc tgtaaaggca cgccagggtg agaaacgccg agaggtactg cgtgatgatc
     6121 gttgcccatg ccaccccgtc gacactcatg ccgactgcaa tgacaaaaaa catgttcaaa
     6181 aatacattgg ccacaccgga gatgaaaagg taatacgtag gatggcggct gtcacctact
     6241 gatcgcagta tggctgccgc aaagttgtac accatgttgg cgggtactcc caggaagtat
     6301 atgcgcatgt acagcaccga caagtcaatg atgtcttcag gagtacccat ccaggccagc
     6361 ataggcttgc acaggacaag ccctgctgcc atcacgatca caccgcttat gatgctgaca
     6421 gcgatcgaag tatggactga ccggcttact tcttccggat ggccggcccc gtaatcctgt
     6481 gccacgatga cgctcgtacc cactgacagt cccatgaacg tattgaccag caaattgatt
     6541 agcgcgcctg tggcacctac cgccgcgagc gcctctttcc ctgaaaactg ccctacgatt
     6601 atcatatcgg ctgcgttgaa cagcaactgc aggatgttca ttgccatgat aggcagcgcg
     6661 aatatcagga tcttgccgaa cagcggtccg ttgcacatat ccatgtcatg atgttttgaa
     6721 atgtccaagt aaatctcttc cttcgatata ttgaatattt tgatctttac gcagtgtcta
     6781 tcctgcctgc ttctgcgtca taaccaaatc gccattgttt acataacagg agtccgactt
     6841 tgttgataaa tatcataaca caataagcat tccagttcca tgtagtatcc tgtgcaatgt
     6901 ttgtaaattc tgcatatgtt aacacaaaca ccccactaag cgagaaaatt ttatgcaatt
     6961 aatcaaaaac accgtctcaa ttgatatgag acggtgttcc ggcgggacga tcccttgccg
     7021 ttttcagtag ttctgcttcc tgacctcgaa gaagctcttc ggatgtccgc acacagggca
     7081 aacctcggga gctttctttc ccattacaag gtgaccgcag ttgcggcatt cccaaatggt
     7141 catctcggat ttttcgaaca cctgctgcat ctccacattg ctgagaagct tgcggtatct
     7201 ttcctcatga gctttctcga tcgccgctac catcctgaac tgctttgcaa gtacggtaaa
     7261 gccttcttcc tcggcatcct ttgcaaaacg ttcatacata tcggtccatt cataattttc
     7321 gccattggcg gcactgagca ggttctcagc cgtgcctccg agctcgccga gagccttgaa
     7381 ccagagtttt gcatgctctt tctcattatt ggcagtctct tcgaaaattg cagcgatctg
     7441 ctcatatccc tcttttctcg ctaccgaagc gaaataggtg tacttgttcc ttgcctgcga
     7501 ctcgccggca aatgcttccc aaagattctt ttctgttttt gttcctttca gatccataac
     7561 taactctcct tttccatatg attttttatt tgcaattttc acaaataaat ttcagattca
     7621 ggtcataccc ggtgatctcg atgcctgtct gcctctgcag aaactctctc aggccttcga
     7681 tgaacatgtc atgcaccgtc ccgcacttct ggcagacgag atgctcatgt ggtattgtcg
     7741 acttgtcgta cctgtccggt gaattgggaa tagggatgcg ccttatctct ccggcatccg
     7801 tcataagacc aaggttcctg taaacggtgc ctatggcaat ggaaggcatc tccttctttg
     7861 cttttatata gatttcctcg gccgtcatat gctcattgga cgtttttata atgttcagta
     7921 tggtttttct ttgtcttgtc aattgagcac ctcaggaata agaattattc ttaattttat
     7981 tatatataaa ataaccccga tgtcaatatc tgaaccagga ttttttatga tttatttact
     8041 aaaatatata ccgatagttt gcggcttaaa atgtactgca ttattcctta tcatattatc
     8101 tcaccagccg gaaggaagtg aagacccacc tgccgtcgat atactcaagc ggaaaatcat
     8161 actgctctgt gcctgccacc gtttcctcga aaggatcgtc gcagacttcc accgtgaccc
     8221 ggtatatgac ctttttatct ccttcgcgga tcatctcgta tgtttcctcc cctttcgtta
     8281 tgtcgctgcc cctgcccgcg gagatagtgt acaatgatcc gtcaaagtcg cgataaagca
     8341 ggatacctga gccatcgccg gccagaaggc gttcaacgat ttcatctgtg aacattgcct
     8401 gcaggtaaga tctgaggtct tcataagttt taatcgtatc atgggtcacc ctgtagtacc
     8461 ggtacccgtc tgcttctttt acatcttcgt aatccgcagg catggtgccg aattcgaacc
     8521 agccgaaagc ctcgaccgcc ttttggtaag ctcctgcgac ctcttcatcc gtaagcgggg
     8581 atatttcagc agcacccgtt tcgcttggac cttcaacggc aacgtctccg cttccgccag
     8641 gctgtaccgt cgcagacgcg ctgccatcat cctgtctcac atccatatct gtactgccgc
     8701 cgacatgagt gtctgttttc acgttgccgc tgtatttgat gggaacacag cctgccgcca
     8761 taactgtgat gagcaggatg atgataaata ccatcatcag tttgccggct gccattttat
     8821 ttgcctcaag ttcctcgtca gtatatatat ctttcctccg cacaacgttc ctccccatct
     8881 cagcatcccc tggtattgcc tgcagagccg gcacaggacc gccagcctgt ttcaagcatt
     8941 tttcgcataa tattccaaag gttttcagtc attattatat tatataatta taaaaaatgc
     9001 actgtttcct atcaggccgg aattatcact ttattctgca tccgttctgg tttgcggcgc
     9061 tgaataagtt ttgcctgtat ggcaaataag ttattctgaa acgataaagc cgttaagctt
     9121 ataaaaatcc ataaccatgc aaaagtggcg gataaaaccg cactgaagag gaggaaatga
     9181 agaacccggg attcactccc ggatctccaa tggaaccgtc tccggattgc ccggtcctgc
     9241 ttttttataa atttcattta tctcatcttc gtactcaagc aatacttcta caatctccgg
     9301 gtcgtaatgg gtcccggcgc cctgcctgat cacttcgagg ctctcagcgt gggagagggc
     9361 gtctttgtag actctttttg accgcagtgc atcgtatgtg tccgctacag ccattatcct
     9421 tgccgacagc gggatcttat cgcccgacag tccgtccgga tacccggtgc cgtcccactt
     9481 ctcatggtgg tgccttgcga tgttcaccgc catttccagg aacttgtacc ccgaatatct
     9541 ctgctttact tccagcaagg tttcttcgcc gatgatcgta tgtgtcttca tgatctcaaa
     9601 ttcttccggc gtcagtttgc cgggtttgag cagtatgctg tccggtatcc cgacctttcc
     9661 aatatcgtgc agcggagccg ccttgaagat atattcgata taactgtcat tgatatatcc
     9721 cctgtatttt tccctgctgc gaagtttgtc tgccagcagc ctgcagagtt ccgcaactct
     9781 caatacgtgt tctccggtat tgtaatcccg cgattcagcc agtttgacca gcgaatatat
     9841 cgtggccata tgcgcctcga atatttcgtc gtcccttttc ctgacattag tgagttcctg
     9901 cctgtaatga tcggcaaaaa agccgatcac atatgcgatt atcacatata tgactgttct
     9961 tatgatccag ttgaccggag gctgcattat attgtgctcc gcatccatcg gcatgaacgg
    10021 cccgagcagc agtccgcctg ctattgcgtt gataatgccg tgttttttgc cgttggtgga
    10081 cgaaacgata gtgatcggta tatgcataag gtgcgcatat accttacttg tcccgcctgc
    10141 agtataaaca acaaaggcta taattgcagc catcgcatat gaaagcacca aaatcagact
    10201 gcttctgatg ctgatatctt ttgaattcag tcttataccg aactgcatcc ctacacccgc
    10261 cagacagtat ttatgaacat gcgcttccct gaaaaaatga taattcattc agattcttgc
    10321 ggtaatttgc attattctgc gtttcttctt ataaatttta ccacattttc cggcggttta
    10381 aatcattatt ttctgaattt acgtccgatc tccggtatta tcccggtttg aaaccgcata
    10441 ttcgctgtac ttcgccaggc gcgtcccgag atcgtcatcg gcctcataaa catatggaaa
    10501 agcatcgagc catgaatacc gttcaagaaa aggttcggcc ggggatggcc ccaggcagag
    10561 cgaaccgcct tcggtactca tgagaagtgt gcttttgccg aggtaagcat ttacatcatc
    10621 cgcaaaagcg atcatcttag gcacatcgag ttcgcatgtc ccatttatgt cgaattcaag
    10681 ggaattggtc ctgtaaactt tctgcctgcc gtcttccagg gtgaaaaggg tatatgcgta
    10741 ggtggcatat ccctggtaca tggttggatt gtaacgcaat aaataatacc cgccttcgtg
    10801 ctcacaaagg aacagggaat tccagcccgc gtgcgcggta ttagccgact cgctccatat
    10861 ctcgtttcct ttgctgtcga tgatgcgcag cgttatatag ttagtgcctt ccattaggga
    10921 tttgtctatg cagatctcat cttcatcgcc atctctgtca agatcggcaa cggcaaccag
    10981 ttccatgtca tcttttccgg caatcccggg atccctcccg gtgatcaggg tatcggttcc
    11041 gctctcgccg ccaggcatac ccggtatgtc gccgtcttcc gaaatttcga actgttcttc
    11101 ggtgcccgga gtctcggcag gtacagctct gccgcccgcg acgccgatac ccaggacaat
    11161 tacgaatatg gcggacatca gtacgaccca ctcaagatat tttttggagt tcggcatatt
    11221 catgctcctc tcttttgtat ctctctgctt ttaccacgtt gccggatttc agcctgatag
    11281 tccctggttt taactcatcc atgcccctgc tgcccatgta cgtatatgaa agggatgtgc
    11341 ctgattgtta tgacatgcag ccatattcct caaggttcct caggcttatc cccaaactct
    11401 cgaacggatc tcgcaggcag atgtcctgct ccaccgcggc ccatttcact cctgtctccc
    11461 tgcatgcatc tatgatcccg ttccagttga gattgccttc gccgacttcg gccatagttt
    11521 gtttcccctg gtgcattacc atgtccttga agtggatgac atccacgcgg cccttcagct
    11581 tcctgatcca ttcgatggga tcggctcccc cggcctgtac ccagtaagta tcgatttcga
    11641 acccaaagta ttccgggtcc gtttcagtaa gcaggatctg cattcctgtc atcccgtcga
    11701 atttatggaa ttcgaaattg tggttatggt aaataaatcg cagtccgtta cgactcaaaa
    11761 ccttcccgaa cccgttggct attcctgcaa attcaacgta ttcttccctg ctgccgcgga
    11821 aacgttccgg catggcgcca aggccgacat acctgcagtt ccacatctta tgaagctcga
    11881 tcatccagtc catttcacgc tccagttggt caaagccgac atgtgtggca catatcgcca
    11941 gccccagttc ctctgccgcc tttttcacca gctccgcctt ttcccggtcg atatagcctg
    12001 tcccggacac ctgcaccgca tcgtatccga tctcttttac cctcttaagt gtatttacag
    12061 tatccttagg ggttttcata aactccctga ctgtgtaaag ctgcgctgcg attctaagtt
    12121 cactcatatt tcttccccct gtcagtgttt cactcgtaaa gcctgcatat atccggatca
    12181 gttttgtggt acagtcaata tcaatttccg cggtcctgcc agatccaata ttcagcgacc
    12241 tgccttctct ttcaggctat ttcatcatcc tgcagccttt gagccgatat tcacggccgg
    12301 tcacggacga ttcatatatg cctctgacca tttcaacgga aacagccgca tcgcaaacac
    12361 tgatatagtc cgtccagtcg tacatgagac actcataaaa gcgcctgata agcttctcgt
    12421 ggctcaggcc ccagtatgct ttctcaccgt aagccggctg gtcgcttgcc agcagttctc
    12481 ttttaccgtc cctggcgata tacagttctc cgtcccatat caggagcgac cctttctcgc
    12541 agtggatctc gataatcaca ggcgagttgt ccgtaaagca aaccgtggca tagagaattc
    12601 cccttgcgcc gtttttgaac caaatggaag cttcggccgt atcctccacg tcgatcatac
    12661 cgcggaaaca ccgggtgtct gcgtggccgc ttatccgctc cacttcaccg ccgaaccatt
    12721 gcatcaggtc aagcgtatgg agagcctggt ttatgaggac gcctcctcct gctttttccc
    12781 tgtcccctct ccagccgctt tcggtatagt aggcttcatt cctgcaccag gtaacgatcc
    12841 cctttatccc ttttaccttc cccagagtcc cggaatcgat cacttccttg gcttttacag
    12901 atgtcggatt gaaacggttc tggaagcaaa cggccagatg ctttccgcaa gccctggctg
    12961 tttccgccat tttcgctgca tcctccacgt taacggcaac gggcttttca gtcagtacat
    13021 gctttccggc tttcagcgca tctatagcca tggaagcatg gagataatgg ggcgtacaga
    13081 tatggaccgc ctgtatcccc ggatctttca gcattttcct gtaatcagtg taaaagtcac
    13141 agccatgccg gatggcagct tctttcgctg cactttcctt aatgtccgca actgccttta
    13201 gttcagcgat ccatgatttt tttactgcat cagcgtgtac tccgaatatt gagccgcatc
    13261 cgataatgcc gactccgatt ttgctcatgt attttcactt ccgtctatat aatcagcctg
    13321 tattgtgttg tgcatgtact gtggccggac tgtattgccc ggcctgtgtg cgtcaactcc
    13381 tgacggcaga ttatccggca tgcaggataa acatatccgg ttatatgcga gcaacgcacg
    13441 tcctgttaag aaggaaagac atatcatcat cgcatgtcag gttccctgcc tgtcaatacg
    13501 tcccgctgac atccagggtc acatcttttg catccttttt aaaggtcgaa gtttttaccc
    13561 tctcctgcag tttatcatag aacagatcct cgtctatggg cagttccacc cagccgtctg
    13621 tccatgcgga cagatgcatc gcattggata tcgtcagccc aaggatccct tcctcgccgg
    13681 gagccagcag cggcgtcccc ttggtgatgg cgtcgatcca gttgttcatt atgccaacat
    13741 gatcggtctg atgcccattt acggggatct cacacttcca gcattccggc tctccaaatc
    13801 cgcctttgta ttctttattg aactgcctct ccgatacgcg gagccggtgg aatactattt
    13861 tcccgtcctc tatcacgatc tttccccggt ctccggttat ctcaaggcgg tttgtaccgg
    13921 gcgtgtcaca tgtggatgtg atgaacactc cggttgcacc gttttcatac tccacgtatg
    13981 ccgtgacatc atcttccact tctatatcat gcatcttgcc aaagctcatg aatgcccgga
    14041 cccggacagg catcccgcat atccattgcc acagatccag ctgatggggc gcctggttca
    14101 gaaggacccc tccgccctct ccgctccagg tagctctcca tccgccgctg tcatagtagc
    14161 tctgtgaacg gtaccaggat gtgatgatcc agttggtcct gcggatatcc ccgagttctc
    14221 cgctttttac cagttccctt gctttctgat acagcgggtt agtcctctgg ttgtacatga
    14281 tgccgaagac cttgccgctt tgggccgcgg cctcgttcat ctcccttacc tgcttcgtgt
    14341 aaacgccggc cggtttttcc accaacacat gcaggccgtt ttcaaaagcc ctgaccgcaa
    14401 gagggggatg gtcgtagtgc ggagtacaga ccattaccgc atcgatcgct ttcgccctga
    14461 acagttcatc cggagtgctg aacctttgca catcttcccc caacgccttc ttcgcccatt
    14521 ccagcctttc gggccgcagg tcgcaaaccg ccgccagcct ggcgtccggt actttccctg
    14581 cagcaaggtt catcgcatgg gagcttccca tattcccgat gccaatgatt cctacacgta
    14641 ccttatccat tatttcgcct ccccggtgat ttcctttatc agcttcttca gagcatatgc
    14701 cgctatggca aacttatcag gcccggaatc ctccgcgctt gtctccgctt tcctgttttc
    14761 aagttcagca aagcccacga agttccacag atgcggttct attgaaagaa atccgtcaaa
    14821 gcctcttttg taaaggctct ccagtatctc cttcaccttc ccgtctccct gtccggcagg
    14881 gaccacgtga tgatcgctgt atacggcatc cttgatatgc aggtagacga catgctcctt
    14941 cagcagttca taggcctccg gataagtctc cacatcgcac tgtacaaagt tggccggatc
    15001 aaatatgagc ttcaggtttt cgctgtccat ctcctttacc aggtcaagac accgctgagg
    15061 cgtatcgccg tagataccct tctcgttttc atggagcagc gttatcccgg tcccttccgc
    15121 cgcgtccagg aaccttctcc accggtccat gacttcatcc cggtaagttt caggcttttc
    15181 tccctcaggg atataaaaac tgaacatacg gatataccgt gtctccaaaa tttttgcaat
    15241 ctccagtgta tgcctgaaca gttccaggtg cggcccgaaa tcgtcggtga tatttatctt
    15301 ccctatcggc gacccgacag cggagatcct gaaacccctt ttgtcgagtt gtttctttat
    15361 tcccttcact tcttccggcg agtgctgcgt gatgtttttc ccgttcaccc cacgcatttc
    15421 gatatagctg atcccgtgct gttccaggac atccatctgc acgtccagtc ccggatctat
    15481 ctcatctgca aaagccgaca aaatgaacct gctcattttc atcaccccgc ctttgttctc
    15541 atggaaaccc tacatactat tatagagaat actccggatt gtctctttga ctttattgct
    15601 tcaaacattg caaattttgc tgtataatat aacaaggttc atacatgcct ggcgccttgt
    15661 tgaaacagcg ctgatgtatg ggattctttt ttgaataatg cgttgctgca gcttgaagga
    15721 acggtatttt tatggacccg catttgtatg aatattcgta tgagaaccag ttcaatatca
    15781 gccatacggc cgatccgaaa gccccggcgg aatacccggt ccatatccat gacaggtacg
    15841 agatatatta cttcctttca ggagatgtga catattttat agaagggcag agctatcccc
    15901 tcagcaggca tgatcttctt gtaatcaaca ccggagaact ccacaggccg gttttcaact
    15961 catccaggcc atatgagcgg atcacgatcc acttccggcc ggaatatatc tcttccttcc
    16021 agcagaagga ctacaacctg ctttattgtt ttgagcgcag gaaactgggg catcaaaacc
    16081 ggatcgacgg ttccgatatc atcgaaagcc aggcagacca gtacttttat aaaatcagaa
    16141 agtatgccac ggaacgggtc cgcgaaagcg accttatgat aaaaacgtat tttttgcaga
    16201 tgctcgttgc tctaaaccgt atttttgcag acaaaagcac aactaccaaa aacaccatgg
    16261 cctatgacgt gaaaatagtt gaaatcctgg actacatcaa caaccacctg catgagcaga
    16321 taacgctgga ttcgctggaa gagaagttct atgtcagtaa gtactacctc tgtcacctgt
    16381 ttaagaaaaa tacaggggtg accgtaggag aatatattgc ttacaaaagg atacttaaag
    16441 ccaaggaact gctgatcatg ggcgccccgg tccttgaaac ctgtcacacc gtgggtttca
    16501 gggactattc gaatttctac aaaaccttca ggcgcctgga aggggtttcg ccaagggagt
    16561 ataaacaatg aacattgcct gaagctcgtc tgttcggcag taatttatcc tccatccaag
    16621 gctgtcagca gaaatttata gtgtgttttg aaaggcataa gcatagcaga ttttgttttt
    16681 accattatcc tgaattgctc aagcggtaca tatgatactt ctttcacttc attatcgctg
    16741 aaagagtact cttctacagg aatatctttt ctataaagaa aaacatcatg tatttcattt
    16801 tcaaaatatc catcagcctt caaatattcc cggcaagtaa agagatattg tagtttgtct
    16861 atatccaaat caatgccaag ttcttctttc ccttcccgta tagctgcttc cagggatgtt
    16921 tcgccagtcc gtatatgtcc cgaaaaagat atatcaagca tatttgggaa cttagcgtga
    16981 ctattgcggg tttgcagcag tatctcatta tttgaattaa tgatccatac acaaacacct
    17041 ttatgaataa gtgcttttct atgagcttcg tatttttctt cggtgccgca tttctgacct
    17101 aattcgtcat aaatatcgat cagttccata tagcaatacc tccgtataat cctgtttgct
    17161 gcccttatgt tatgaatcat accacaattt agtagttttt ctcaataaat actataaagg
    17221 cggtcgcgtc aacttgtttt aggttttgtg ttactgtttg cccctccttt taggccaagg
    17281 atcatatagc aaatccatta tctcgtatcg atctaagtgt ccgtgtcagc agagttactt
    17341 ttccgctatt cagcagtgct atgttatgta tcagctctct tgaggcatcc ttgtacactt
    17401 ttgttgcttt actc
//
LOCUS       DAOSCC010000019        17387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2263_length_17387_cov_4.380395, whole genome shotgun sequence.
ACCESSION   DAOSCC010000019 DAOSCC010000000
VERSION     DAOSCC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17387
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2263_length_17387_cov_4.380395"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLG72_02105"
     CDS             complement(<1..682)
                     /locus_tag="PLG72_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOJ79629.1"
                     /translation="MSVDAVTNQKTIQQIIKENESKTGSRDTTDELGKDDFLNLLVTS
                     LRFQDPLNPTDDKEFIAQLAQFSALEQMQNLNTSFTATRAYSLIGKNILANVKDSSTG
                     EVTEVSGDVTSVTYSGGKYYVVVRGMEVPVEDIIEVAEGAYASQNNLSQFTGIIGCLV
                     NGIVYNPLDGDMIKVSGVVKSLQKGIYEDYAVLDGVSVEIAGLNTTVKTTDVEYVRNK
                     LTTAWENGE"
     gene            complement(694..2268)
                     /locus_tag="PLG72_02110"
     CDS             complement(694..2268)
                     /locus_tag="PLG72_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ79630.1"
                     /translation="MQLAEIFTGNIAAGMDVPAPRTNGSQTRSYGGNSAGFGKILETT
                     MSRTQNRPGRTQGASARRSDPSDFYDDSSLQGPQRSPKEKFERPSVSDTQNRRKAENL
                     NDDERPDTESNKRAESVDMLEVLAQLTGLDRTALKEILTALGISEEALSAQQGFAQTA
                     AGLAEMLGLDTVQKDALAELMQLIEDAGGIWNTADLAAGHGDGQPAGDMQAGIAINAA
                     GFPDAVQKLLASVSEISGQLRAAILRKLNGHAAKLEDDGGDGNAEVRDAVLNMLEKVR
                     FSRKNAVGQGTGDVSESEQAGDESLSDSAKAAPNAAEDAEMAGTVVANESGTAQKPVL
                     QQDTGTMNDGEMPDAAMIAVQQNNPGPDAAPQAAAERTANQTVPVRQIITQITEKASA
                     ILAHDRSEMVIELKPESLGRLSLKVVTENGIVMAKFVAENSQVQRLLESNMQMLRESL
                     ERQGIVVQDLSVSVRQDGRQARENGPQNRSLSGVRPKGVTPMAAARIPDMSGIAEAPG
                     TGDPWLWQGSTINLTA"
     gene            complement(2299..3102)
                     /locus_tag="PLG72_02115"
     CDS             complement(2299..3102)
                     /locus_tag="PLG72_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79631.1"
                     /translation="MAKPEESAVNKDGKNKKRSKPLSIFLIVLDVIIIIAAFCAVFYF
                     IVYNNVGGVTERYYSSIKSIPFLRLALHEPPDPLNPKYMTQDEIREKYLEFRDEVEKL
                     KEQLAQANARADELNVYKDDYDKLVQEASAKLEELNKREAAIAEKEKELEELKKQVEK
                     SIVNGDKEAFADYYESIDPENAAAIYESVIVERQTAENVKKFAQVYAEMDADSAAAIF
                     EQMGVSKLDMIAETLQAMNRKNAASIIQAMSPDFAARVTEKLNELYRGE"
     gene            complement(3105..3554)
                     /gene="fliJ"
                     /locus_tag="PLG72_02120"
     CDS             complement(3105..3554)
                     /gene="fliJ"
                     /locus_tag="PLG72_02120"
                     /inference="COORDINATES: protein motif:HMM:NF028099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOJ79632.1"
                     /translation="MAKFIFKLQSVLNLRKQKEDSIKNELAAAMRKLEAEKRKLSELE
                     NALEDTVREFNKKAKKATVHELIKFNGYLSLLNSRIKSQKENVNNAAKYVDKVREELV
                     KAMKDRKILEKLKDRQYEDFLLEQKKLEQKTNDEIVSFNYNDSNTGD"
     gene            complement(3593..4933)
                     /gene="fliI"
                     /locus_tag="PLG72_02125"
     CDS             complement(3593..4933)
                     /gene="fliI"
                     /locus_tag="PLG72_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOJ79633.1"
                     /translation="MRADNSRGGTVNIKLQRYHDLLDKSSFVKYFGRVSKVVGLTIES
                     DGPEVDIGSLCKLYASKGKKIIQTEVVGFRDNKVLLMPLGEMSGVGPGNLVVASDSTL
                     RAGVGTELTGRVLDGLGFPIDDKGPFRPEKYYPVDNDPPHPLFRKRITDPLPLGIKAI
                     DGLLTVGKGQRMGIFAGSGVGKSTLMGMIARNTKADVNVIALIGERGREVREFIEKDL
                     GEEGLSRSVVVVATSDQPALIRLKGAFIATAIAEYFRDQGKDVLFMMDSLTRFAMAQR
                     EIGLATGEPPVSRGYTPSVYGIMPRLLERAGNSEKGSITGLYTVLVDGDDLTEPVTDT
                     ARSILDGHIVLSRSLANRNQYPAIDVLASVSRVMPDIITDDHKAAAAEIKKALAVYRD
                     AEDLINIGAYVKGSNEKIDHAISLIDKILGFVQQSTEERFDYDEIIDMMYGILQ"
     gene            complement(4920..5672)
                     /locus_tag="PLG72_02130"
     CDS             complement(4920..5672)
                     /locus_tag="PLG72_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOJ79634.1"
                     /translation="MYNRVFKNNQVTYGRPYQVRLPDNLIKFVNDTDSEHDVTDEESS
                     AISPEELLRKAEKKSEEIIAEAEAEAKRLIEQARAKAEEDAKAIAEEAWQRGYAEGMD
                     AAAAQSRSLLEEAEKIKQDAAEEYEKIMAGMEADILELALKIARKAVAGELKTNRDVI
                     LQLVRSALAECSEKKGAVIRVSPEDSDYLNENRKRLEQLAEGADDTEIRPDAAMKPGE
                     CIVETPLGSIDAGAETRLEKIEEALKEEYEGR"
     gene            complement(5665..6687)
                     /gene="fliG"
                     /locus_tag="PLG72_02135"
     CDS             complement(5665..6687)
                     /gene="fliG"
                     /locus_tag="PLG72_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOJ79635.1"
                     /translation="MARVSGMKTERTGREKAAMLLISLGPERSAEIFKHLKEEEIEQL
                     TLEIANIRTVTPEEKEKVLEEFYQICLAQEYIAEGGISYAKEILEKALGTQKALDVIN
                     KLTVSLQVRPFEFVRKADPAQLLNFIQKEHPQTIALILAYLKPQQAAVVLASLPQDKQ
                     ADVARRIAVMDRTSPEVIKEVERILEKNLSSLVTEDFTAAGGVQAIVNILNTVDRGTE
                     KYIMETLEIEDTDLAEEIRKRMFVFEDILTLDNRSIQRFLREVDNNLLAVALKGATEE
                     VQKVIYSNMSKRLAEMIREDIEYMGPVRLKDVEEAQQKIVNIIRKLEDAGEIIISRGG
                     GDEIIV"
     gene            complement(6866..8437)
                     /gene="fliF"
                     /locus_tag="PLG72_02140"
     CDS             complement(6866..8437)
                     /gene="fliF"
                     /locus_tag="PLG72_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOJ79636.1"
                     /translation="MPESIKKYLDQLKEFWESLDKSQKTRFYITSAIVVIAVTISIIV
                     LTRPQKITLFTSSDKKQIGEMINILNENGIWNEAGNDGTSIIIDKKNNNKAQIVLAQS
                     GYPKEGFTFADAISSIGLTTTQQDREQIWMHQKISDLETKLEMLDNIEEAAVTLAVPE
                     KSIFLNASNEQPRPTAYVMVRPREKLSQTQVQGIVMLVSRSVENLDPKDVTVVDNNSN
                     ILNANYEDDSIGAVSSQEELRRKKELELQQKVLDYFSVGQFDSFDTLRVVANVVLDFD
                     KEKSQIKRITNPEGMDGGAVISSSISEEIVKNAEGGGEPGLDSNPGGVTTYQIGEEGA
                     SEYSNRQEDINYGYDEVLSETEKAAGKMIPEESGLALSLWYGQKVTDDSRLSDEFISE
                     LRTALSTATGIPARNISISKFKLAPLETVEKTTADKIRELINDYGFFALMLLLIIGML
                     IFGIPRRKETGEPAVAQAASGPRFVVPEEEEPLPEIELEERSEIKKQIEKFVKQKPDA
                     VAQLLRNWLSEDWDT"
     gene            complement(8598..8903)
                     /gene="fliE"
                     /locus_tag="PLG72_02145"
     CDS             complement(8598..8903)
                     /gene="fliE"
                     /locus_tag="PLG72_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOJ79637.1"
                     /translation="MAFKVGTVDSLAPSRSIFETENRSATAGAIPFSDYLKEALYRTN
                     DLLLEADKLADDFAAGRTDNIHQVILAGEKANIALQFTLQIRNKLLDAYTEIMRMQI"
     gene            complement(8971..9414)
                     /gene="flgC"
                     /locus_tag="PLG72_02150"
     CDS             complement(8971..9414)
                     /gene="flgC"
                     /locus_tag="PLG72_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOJ79638.1"
                     /translation="MSYFGALDIGASALTAQRLRMDIISQNIANANTTRTEDSTPYRR
                     RVVVFQESSDGIPFSEYLSESSKARYLGKGVKVSRIVEDTSPFKRVYDPGHPDADEDG
                     YVQMPNVDVVTEMVNMISATRAYEANVTAMNSTKSMAMKALEIGR"
     gene            complement(9418..9828)
                     /gene="flgB"
                     /locus_tag="PLG72_02155"
     CDS             complement(9418..9828)
                     /gene="flgB"
                     /locus_tag="PLG72_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOJ79639.1"
                     /translation="MFDKLTSNLRLREKALDAAWIRNEVIAQNIANVDTPGYKKSSVA
                     FEEYLQGASEKSGFKGNTTDRRHIPIGKSNENAKIRIVRDNSELSTRLDGNNVDIEAE
                     MAEMAKNSIRYYTLVQSVSSSYQRIRTVINEGRR"
     gene            complement(10143..11459)
                     /gene="trmFO"
                     /locus_tag="PLG72_02160"
     CDS             complement(10143..11459)
                     /gene="trmFO"
                     /locus_tag="PLG72_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOJ79640.1"
                     /translation="MAERYINVIGAGLAGCEAAWQAAKRGVQVRLFEMKPKKYTPAHK
                     MDFFAELVCSNSLRSDSLTNAVGLLKEEMRRLDSIIMKCADETRLPAGGALAVDREGF
                     SKAVTKAVKDHPNVIVYSEEVTSLPEDGITVVATGPLTSDAFAEHIMKVTGRDSLYFY
                     DAAAPIVTYESIDMTKAFRASRYGKGTDDYINCPMDREQYELFWNELVNAETVPIRDF
                     EKMILFSGCMPVESMAANGRDTLRFGPLKPVGIIDPATGKEPYAVVQLRQDNKAGTLY
                     NMVGFQTHLRWPEQKRVFRLIPGLENAEFIRYGVMHRNIYIDSPGLIDPTYRMRSRPE
                     LYFAGQITGVEGYVESASSGLVAGINAARQCLGESLVIFPENTAIGSLAAYVSGNSVG
                     RLQPMNASFGLIRAPEQRIRNKNEKHRIMAEIALDQIEKIKMSEKI"
     gene            complement(11568..13688)
                     /gene="topA"
                     /locus_tag="PLG72_02165"
     CDS             complement(11568..13688)
                     /gene="topA"
                     /locus_tag="PLG72_02165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOJ79641.1"
                     /translation="MANNLVIVESPAKANTISKFLGKDYKIVASVGHVRDLPKSQMGV
                     DIENDFEPKYITIRGKGDIISKLRKEARNSKKILLATDPDREGEAISWHLAQLLNIDE
                     NEECRVTFNEITKNAVRNAIKQPRKINMDLVNAQQARRVLDRIVGYKISPLLWRKVRK
                     GLSAGRVQSVAARLVCDREEEIENFIPEEYWTITALLHKKKSSRTFEAKFFGKMDQKI
                     ELKDEEQVREILKQLEGKEYIVHKVKYGEKKKTPPAPFTTSTLQQEASRKLGFPTKKT
                     MMIAQQLYEGLEVKGHGSVGLVTYIRTDSTRISAEAQQAAREYIHASYGEKYLPNEPR
                     VYKNRSASQDAHEAIRPTYVELDPASVKGSLTNDQYKLYSLIWSRFIASQMASAAYDT
                     IAADILAGDLLFKANGSKMRFDGYLTVYTEGRDDDTKENEVSIPDLEQDEMLMLKKIE
                     DKQHLTQPPMRYTEATLVKALEEKGIGRPSTYAPIITTILARGYVVKDKKFLVPTELG
                     RIVNELMKNNFKDIVDIQFTAQLEQKLDDVEEGAKKWVELMKEFYEPFAKTLKDAETR
                     IENIDIPDEVTDVICEKCGRNMVIKTGRYGKFLACPGFPECRNAKPILEEAGVNCPLC
                     GGKVLIKKTKTKKSYLGCENYPECKFMSWDMPSGEMCPKCGNFMVKKYSGRKVTLVCS
                     NEKCGFTKSPDRKKQPEEAESSDE"
     gene            complement(13804..14997)
                     /gene="dprA"
                     /locus_tag="PLG72_02170"
     CDS             complement(13804..14997)
                     /gene="dprA"
                     /locus_tag="PLG72_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ79642.1"
                     /translation="MNAVAELKPVGISSSMKIDLMDKILQQDVGDEAMSNLKYWIWLS
                     SMVNASPGKKYQLLQYFRDPALVWEAGEADLAASGLCSKKLITHLIDKTARKETERAF
                     ESVKKCDADVITIYDRTYPELLKHIADPPLILYCRGKPEPDETCMAVVGSRRATWYGL
                     DMAKRLAGDLARHGVTVVSGMARGIDSQAHKGALEAGGRTIAVLGCGIDIAYPPENRG
                     LMEEIIRHGAVISEYLPGTEPARYNFPARNRIISGISLGVTIVEAGEKSGSLITAEYA
                     LDQGRDVFAVPGNINSYNSAGTNRLIRDGAKIVTGVGDILDELNIRHDSKNTYSSAKK
                     LAVMKLNRDEKTIAQRLLNGPVHLDVICRDCGISVQMASSILLMLELSGFVEQLPGKY
                     YRLVE"
     gene            complement(14994..16526)
                     /locus_tag="PLG72_02175"
     CDS             complement(14994..16526)
                     /locus_tag="PLG72_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOJ79643.1"
                     /translation="MYSKIISCGVLGIDGYIVEIETDISNGLPAFEIVGLGDTAVREA
                     RERVRSAVKNSGYEFPLKKITLNMAPANIKKEGSSFDLAIAMGILTSSGYIDQSAASK
                     SMFIGELALDGGIKPVKGVLPMAVCAHRNGFRFLFLPEANAREASVVKELHIVPVKDL
                     RETAAIINGEIEIKYFEDTGGEVVPVYSDTVDFADVKGQENVKRALEVAASGAHNCLM
                     LGAPGCGKTMLARRLPTILPSMTFEEALEVTKIHSIAGILPPGVSLVNTRPFRSPHHS
                     ISDTGLVGGGSIPKPGEISLAHYGVLFLDELPEFSKKALEALRQPLEDGVITISRVNA
                     SLTYPARTTLICAANPCRCGYFLDQPGKCTCTPKQIQQYLSRLSGPLLDRLDIHIEVS
                     PVKYAELESDKNVESSLAIRERVERTRKIQLDRYKGLNIYSNSQLQPAMFAKFCRLDK
                     KCRSILRDVFDRLGMSARAHSRILKVARTIADMDMSEDIKPVHLTEAIQYRSLDRKAW
                     NI"
     gene            complement(17004..>17387)
                     /gene="nrdR"
                     /locus_tag="PLG72_02180"
     CDS             complement(17004..>17387)
                     /gene="nrdR"
                     /locus_tag="PLG72_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOJ79644.1"
                     /translation="KVESIPLMVIKKDKTRQPFDREKLINGLLRACEKRPVSVDTLEK
                     LADEIELQILNSLKREITTKEIGEMVMARLKDIDEVAYVRFASVYRQFKDINTFMEEL
                     HKLLKENEKLQENEQQEENGQDDHK"
BASE COUNT         3502 a         4758 c         3776 g         5351 t
ORIGIN      
        1 actcgccgtt ttcccaggcg gtggtcagct tgttccttac gtactcgaca tccgtcgtct
       61 tgaccgtcgt gttcagtcct gcaatctcga cgcttacgcc gtccagtacg gcataatctt
      121 cgtagatgcc tttctggaga gatttgacga ctccgctgac cttgatcata tcaccgtcca
      181 gcggattgta aacgatgccg ttcacaaggc agccgataat gcctgtaaac tgggacaggt
      241 tgttctgaga agcatatgct ccttcagcca cctcgattat atcttccacc ggcacctcca
      301 tgccccggac aactacataa tatttcccgc cggaatatgt aacgctggtc acatctcctg
      361 acacctcggt aacctcacct gtcgaggaat ccttcacatt ggcaaggata ttcttcccaa
      421 taaggctgta tgcccttgtt gcagtaaagc tcgtgttcag gttctgcatc tgttccagtg
      481 cgctgaactg cgccaactgc gcgatgaact ccttgtcgtc cgtagggttc aatggatcct
      541 ggaatctcag tgaagtcacg aggaggttga gaaaatcgtc tttcccaagc tcgtcggtgg
      601 tgtccctgct gccggtcttt gattcatttt cttttattat ctgctgtatc gttttctgat
      661 tggtcactgc atcaacgctc attcattcac ctcctatgcc gtcaggttga tcgtgctgcc
      721 ctgccacagc catggatcgc cggttcccgg agcttctgca atgcctgaca tatccggtat
      781 cctggctgcc gccatcggtg tgacgccttt aggccggaca cctgacaggc tcctgttctg
      841 cggtccgttc tcccgcgcct gtcttccgtc ctgcctgaca gatacgctga gatcctgcac
      901 gacgataccc tgtctttcca gcgactccct cagcatctgc atgttggatt ccagcagcct
      961 ttgcacctgt gagttttctg ccacgaattt cgccatgact atgccgttct cggtaacgac
     1021 ctttagcgaa agccttccga ggctctccgg cttaagttcg ataaccatct ccgacctgtc
     1081 gtgcgccagt atggcagagg ccttctcagt tatctgagtg atgatctgcc ttacgggtac
     1141 cgtctggttt gcggttcttt cagcagctgc ctgcggcgct gcatcagggc cggggttatt
     1201 ctgctgtacc gcaatcatcg cggcatccgg catttcccca tcgttcatcg tcccggtatc
     1261 ctgctgcagc acgggttttt gagcagtacc gctttcattt gccacgacgg ttccggccat
     1321 ttccgcatct tccgctgcat tcggggccgc tttggcgctg tcggacaatg actcatcgcc
     1381 tgcctgctcc gattcggata catcgccggt accctggccg actgcatttt tcctgctgaa
     1441 acgcactttc tccagcatgt tcagtaccgc atccctgact tctgcatttc cgtctccgcc
     1501 gtcatcttcc agctttgcgg catgcccgtt cagtttccgg agtatagcgg cacgaagttg
     1561 accggaaatt tccgacaccg acgcgagcag cttctggact gcatcgggaa aacctgcggc
     1621 gttaatggct atgccggcct gcatgtctcc ggctggttgt ccgtcaccgt gaccggcagc
     1681 caggtcagcc gtattccata tgccgcctgc atcttcgatg agctgcatca attccgccag
     1741 ggcatctttc tgcaccgtgt caaggccaag catttccgca agtccggccg cggtttgagc
     1801 gaaaccttgt tgagcggaca gtgcttcctc agatatgcca agtgccgtca gtatctcttt
     1861 cagtgccgtc ctgtccaacc cggtaagctg tgccaatacc tccagcatgt cgacagactc
     1921 agctcgctta ttgctttcgg tatcaggcct ttcatcatca ttcagatttt ctgcttttct
     1981 cctgttctgc gtgtcagaaa cggacggtct ttcaaatttc tctttgggag atctttgcgg
     2041 tccctgcagg ctgctgtcgt catagaaatc cgaaggatcg ctccgcctcg cggaagcacc
     2101 ctgtgttctg ccaggcctgt tttgtgtcct tgacattgtc gtttcgagta tcttcccgaa
     2161 tcccgcagaa ttccctccat agctgcgggt ctggctgccg ttggtcctgg gtgcgggaac
     2221 atccatacct gctgcaatgt tgccggtaaa tatctctgcg agttgcaatt tttcacctcc
     2281 tttcgaaaga ttgatatgtt attcccccct gtacagctca ttgagttttt cagtaactct
     2341 cgctgcaaaa tcaggagaca ttgcctgtat tatgcttgca gcattcttcc tgttcatggc
     2401 ctggagggtc tcggctatca tatccagctt tgagacgccc atctgttcga aaatagccgc
     2461 tgccgaatct gcatccatct cggcgtacac ctgtgcaaac ttcttcacat tctcggccgt
     2521 ctgccgttcc acgatgacag attcgtatat ggctgcggca ttctccggat ctatggattc
     2581 gtaataatcg gcgaatgctt ctttatcgcc gtttacgatc gatttttcga cctgtttctt
     2641 cagttcttcc agttctttct ctttttcagc aatggccgct tctcgtttat tcaattcctc
     2701 cagctttgcg ctcgcttcct gcacgagctt gtcgtaatca tccttgtaga cgttgagttc
     2761 gtcggccctc gcattcgcct gtgccaactg ttctttcagt ttttcaactt cgtctctgaa
     2821 ttcgagatat ttctcacgta tctcatcctg ggtcatatat ttcggattga gcggatcagg
     2881 cggttcatgg agcgcaagcc tgagaaaagg gatcgacttg atcgaggaat aatacctttc
     2941 tgtcacaccg cctacattgt tgtagactat aaaatagaaa acagcgcaaa aagctgcaat
     3001 gattatgatc acatccaaaa cgatcaggaa tatggaaagg ggtttgctcc tctttttatt
     3061 cttaccatct ttattgacgg cgctttcttc cggttttgcc atgttcaatc tcccgtattg
     3121 ctatcgttgt aattgaagct tacgatttca tcattcgttt tctgttcgag cttcttctgc
     3181 tccagaagga aatcctcgta ctgcctgtcc ttcagctttt ctaggatctt cctgtctttc
     3241 atggccttta caagctcttc tctaacttta tcgacatatt tagcggcatt atttacattt
     3301 tctttttggg atttgatcct ggaattgagt aaagaaaggt atccgttgaa ttttatcaac
     3361 tcatggaccg tcgctttttt cgccttttta ttaaattccc tgacagtgtc ctccagcgcg
     3421 ttttccagtt cggaaagctt gcgtttttct gcttccagct ttcgcattgc ggccgcaagt
     3481 tcgtttttta tgctgtcttc cttctgtttt ctcagattca ggactgactg aagcttgaat
     3541 atgaactttg ccattatact ctcccgcggg ttttttgggg atgtgcggat gatcactgaa
     3601 ggatcccgta catcatatcg attatttcgt catagtcaaa gcgttcctcc gtagactgct
     3661 gcacaaagcc aagtatttta tcgatcaggc ttattgcatg atcgattttt tcattgctgc
     3721 cttttacgta ggcgcctata tttataaggt cttcagcgtc cctgtaaacg gcaagggctt
     3781 ttttgatctc agcggctgcc gccttatgat cgtccgttat aatatcgggc atgacacggc
     3841 tgacgctggc aagtacatcg atggcagggt actggttcct gtttgccagt gaacgtgaaa
     3901 gcactatatg tccgtcaaga atgctccttg cagtgtctgt cacgggctca gtcagatcgt
     3961 caccgtcaac aaggacggta taaagcccgg tgatcgaacc cttttccgaa tttcccgccc
     4021 tttcgagcag cctgggcatt atcccgtata cggacggcgt ataacctctc gacaccgggg
     4081 gttctcccgt tgcaagccct atttctctct gggccattgc aaaacgggta agcgaatcca
     4141 tcataaacag cacatctttc ccctgatccc tgaaatactc ggcgatggcg gtggctatga
     4201 acgcaccctt aagccttatg agagccggct ggtcagatgt ggccacgaca acgacggaac
     4261 gggacagacc ttcctctccc aggtccttct cgatgaattc cctcacttcc ctgccgcgct
     4321 ctccgatcag cgcgatcaca ttcacgtcgg ccttggtgtt tcttgcgatc atccccatga
     4381 gcgtgctttt gccaacgccg ctgccagcaa aaatacccat acgctggcct ttgccgacag
     4441 tcagaagtcc gtctatcgct ttgatgccaa gcggaagcgg atcggttatc cttttcctga
     4501 ataaggggtg cggcgggtcg ttgtctaccg gataatattt ttcaggcctg aaggggcctt
     4561 tgtcgtcaat gggaaaaccg aggccatcca gcacccggcc tgtaagttcc gtgccgacgc
     4621 cggccctgag agttgaatca gaagcgacaa ccagattgcc ggggcctacg cctgacattt
     4681 ctccaagggg cataagcagg actttattat ctctgaagcc tacgacttcc gtctgtatta
     4741 tctttttccc ctttgaagcg tacagtttgc aaagactgcc gatatcgacc tcgggaccgt
     4801 ccgattcgat ggtgaggcct acgactttgg atactcttcc gaaatatttc acaaaacttg
     4861 atttgtccag cagatcgtga tatctctgaa gttttatatt caccgtacca ccccgggaat
     4921 tatctgccct catattcttc tttcagcgcc tcttctatct tttcaaggcg ggtttccgcc
     4981 cctgcgtcga tgcttcccag cggagtctct acaatgcatt cgccgggttt cattgcagcg
     5041 tccggcctga tctcggtgtc gtcggcgccc tcggcaagct gttcgagtct tttcctgttt
     5101 tcatttaggt aatcgctgtc ttccggcgat accctgatga cagcgccttt tttctcggag
     5161 cattccgcca gcgcacttct caccagttgg agtatcacat ccctgttcgt tttgagttcg
     5221 cccgcaaccg ccttgcgggc tatctttaag gcaagctcaa gtatatctgc ttccatgccc
     5281 gccatgatct tttcatattc ctcggccgcg tcctgtttta tcttttctgc ttcttccagt
     5341 aaggacctgc tctgtgccgc cgcagcatcc atcccttcgg cgtagcccct ctgccatgct
     5401 tcttctgcga tggcctttgc atcctcttcg gcttttgccc ttgcctgttc gatcaggcgt
     5461 ttggcttcag cctccgcttc tgctattatt tcctcagact ttttttctgc ctttctcagt
     5521 aattcctcgg gactgattgc ggaggattcc tcatcagtga cgtcgtgttc gctgtcagta
     5581 tcgtttacga actttatcag gttgtcgggc agcctgacct ggtaaggcct gccgtatgtg
     5641 acctggttgt ttttgaacac cctgttatac aattatttca tctcctccgc ccctggaaat
     5701 gatgatctcg ccggcatctt caagtttcct gataatattg acgatcttct gctgagcttc
     5761 ctcaacatcc ttgagcctga caggtcccat atattcgatg tcttccctga tcatttcggc
     5821 aagcctcttt gacatgttcg aatagatgac tttctgtact tcttccgtag ctcccttcag
     5881 tgcgacggca agcaggttgt tgtccacttc tcgcaggaac ctctggatcg aacggttgtc
     5941 cagcgtaagg atatcttcga atacgaacat cctcttcctg atctcctcgg ccaggtcggt
     6001 gtcttcgatc tccagtgttt ccatgatgta cttttcagta cctctgtcaa ccgtattgag
     6061 tatgttgacg atagcctgca caccgcctgc ggcagtaaag tcctcggtta caagcgacga
     6121 gaggttcttt tcgagtatcc tctcgacttc ctttattacc tccggagaag tcctgtccat
     6181 cacagcaatg cgccttgcga catcagcctg cttgtcctgc ggcagggacg caagcaccac
     6241 cgctgcctgc tgcggtttta ggtacgcaag tataagcgct atcgtctgcg gatgttcttt
     6301 ctggataaag ttcagcagtt gggccgggtc agccttcctg acaaattcga acggcctcac
     6361 ctgcagcgat accgtcagct tgttgataac atcgagagct ttctgtgtac ccagcgcttt
     6421 ctccagtatc tccttggcgt agctgatacc gccctctgct atatactcct gggcaaggca
     6481 gatctgatag aactcttcaa ggaccttttc cttttcctcc ggggtcacgg tcctgatatt
     6541 ggcgatttcg agcgtaagct gttctatctc ctcttctttc agatgtttga atatctcggc
     6601 cgacctttca gggcccagtg aaatgaggag catcgcggcc ttctcccggc cggttctttc
     6661 agttttcata ccggatactc ttgccataca gaacgacctc tcaaatcatt atccataata
     6721 acccctcgtc gattgtgttc tccgtcttaa tatatcatct gctatatcat ccgctgctgc
     6781 agaagcattc agggcggttg cagcatcctg tgccacaaaa ctgcagtatt gcccgtttta
     6841 ccccggcttt cccttacttc cggagtcagg tatcccagtc ttcggaaagc cagtttctca
     6901 gcaactgtgc aaccgcatcc ggcttttgct tgacgaattt ttcgatctgt ttcttgattt
     6961 ccgacctttc ttccagttct atctccggca aaggttcttc ctcctcgggg actacgaacc
     7021 tcggcccgct ggcggcctgg gccactgcag gctcccctgt ctcctttctg cgcgggatac
     7081 cgaatatgag catcccgatg atcaggagca gcatcagtgc aaagaatccg taatcgttta
     7141 tcagttccct gatcttgtct gccgtggttt tttcaactgt ttccagcggc gccagcttga
     7201 acttgctgat ggatatgttg cgggcaggta tccccgtggc tgtgctcagc gcagtcctta
     7261 gttcacttat aaactcgtcg ctcagtcgtg aatcatcggt cactttctgc ccgtaccaca
     7321 atgacagcgc caggcccgat tcttcaggga tcatcttgcc cgcagccttt tcagtttcac
     7381 tcaacacttc gtcatatccg tagttgatat cttcctgcct gttcgaatat tcagatgctc
     7441 cctcttcacc tatctgataa gttgttactc ctcccggatt tgaatcaagt cctggttcgc
     7501 cgccgccttc ggcgttcttg acgatctctt cgctgatact gctgcttatg acggctccgc
     7561 cgtccattcc ctcaggattc gttatcctct tgatctgtga cttctccttg tcgaaatcga
     7621 gcactacatt ggctactacc ctcaacgtgt caaaactgtc gaactgcccg acgctgaagt
     7681 aatcgagcac tttctgctgc agttcgagtt ccttcttcct tctgagctct tcctggctgc
     7741 ttacggcgcc gatcgaatca tcttcatagt tagcgttcag gatgttgctg ttgttgtcga
     7801 ccactgtgac gtccttggga tcgaggttct ccacgcttct cgacaccagc atcactatcc
     7861 cctggacctg ggtctgcgaa agcttttctc ttggccttac catcacatat gcggtaggtc
     7921 tcggctgctc attgctggcg ttgagaaaaa tcgacttttc aggcacagcg agcgttactg
     7981 cggcttcttc gatattgtcg agcatttcca gctttgtttc gagatctgat attttctgat
     8041 gcatccagat ctgctccctg tcctgctgcg tcgttgtcag cccgatactc gaaatggcgt
     8101 cggcaaatgt aaagccctcc ttgggataac ccgactgtgc cagcactatc tgtgccttgt
     8161 tgttgttctt tttgtcgatg atgatggagg tgccgtcatt gccggcctcg ttccagatgc
     8221 cgttctcatt gaggatgttg atcatctctc cgatctgttt cttgtcagag cttgtgaaaa
     8281 gtgttatctt ctgcggcctt gtaagtacta ttatggatat ggttacggca atgactacga
     8341 tagctgaagt gatatagaaa cgagtcttct gggatttgtc caggctttcc cagaattctt
     8401 tgagttgatc aagatacttc tttatggatt ccggcacagt ttcaccaccc tgcaacagta
     8461 tttgtcattt gtaaatcata tatggcatca tctgtgtctg tgttttcgta tctgtgtctt
     8521 cctgtttctc tcatcagacc ggtcggcggc ataagccgta agatgccaaa tgcctgatgc
     8581 cctgatatat cgatttatta tatctgcatc ctcattatct ctgtatatgc atcaagcagc
     8641 ttgtttctta tctgtaatgt gaactgcagt gcaatattcg ccttttcacc ggccaggatc
     8701 acctggtgta tgttgtcggt ccgtcctgcc gcaaaatcat ccgccagctt gtcagcttcc
     8761 agcagcaggt catttgtcct gtaaagagct tccttaagat aatccgaaaa cggtatggca
     8821 cctgcagttg cacttctgtt ttcagtttcg aatatggatc ttgatggtgc aagggaatca
     8881 accgtgccaa ccttgaaagc catggcatcc accttccttt aaactgtctt tggtatcttc
     8941 tctatccgtt cgtcgctgtc agtccgttca ttatcttcct atttccagtg ctttcattgc
     9001 catgctcttt gttgagttca tcgctgttac attggcttca taagctcttg tcgccgatat
     9061 catgttgacc atttcagtca cgacatctac attgggcatt tgcacatatc cgtcttcatc
     9121 cgcatcagga tgtcccggat catatactct tttgaacggg ctcgtgtctt caactatcct
     9181 tgaaaccttg acacctttgc cgagatatct tgccttgctg ctttcgctca gatactcgga
     9241 aaaaggtatc ccgtcggaac tctcctggaa aactacgact ctccgcctgt aaggcgtact
     9301 gtcttctgtc cttgtggtat tcgcatttgc gatattctgg gagattatat ccatacgcag
     9361 tctttgtgcc gtaagcgcag atgcaccgat atcaagagca ccaaaatagc tcataaatta
     9421 tctcctccct tcgtttatca cggtccttat cctctgatac gaactgctta cgctttgcac
     9481 cagcgtataa tagcggatac tgttcttggc catttccgcc atttccgctt caatgtccac
     9541 gttgttcccg tcaagacgcg tgctcagttc gctgttgtcc ctgacgatcc ttatttttgc
     9601 gttttcattg ctcttgccta ttgggatatg tctcctgtcg gtcgtgttcc ctttgaatcc
     9661 gcttttttca gatgcgccct gcagatattc ctcgaacgcg acagatgatt tcttgtagcc
     9721 tggtgtatcc acattggcga tgttctgtgc gatgacctca ttgcggatcc aggctgcatc
     9781 aagcgctttt tcgcgcagtc tcagattgct ggtcagctta tcgaacattt gacccgtctc
     9841 ctttgtaaaa ataatgcatt ccaaaactac cagatatata ttatttcgac attatatatc
     9901 caattccttt agcgttttcc ggaatacatt ctcgcacaga tacaatatag cactgattct
     9961 ttagagtaaa ttttagcata tcataatatt atttacaact atataatgtg cctttgagtc
    10021 gttattgtca atatgttgta tcgatttgtc ataaattatg gaaatatgca ttatttatca
    10081 ggatattcag tctcatggtt atgaaagtct tgataaaata ttcagcaaag ttgtttttta
    10141 agctaaattt tttcggacat ctttattttc tcaatctggt ccagggcaat ttccgccatt
    10201 atcctgtgct tttcattctt gtttcttatc cgctgctcag gcgccctgat caatccgaag
    10261 ctggcattca taggctgcag ccgtcctacc gaattaccgg acacataggc tgccagtgaa
    10321 cctatcgccg tattttcagg gaaaatcacc aaactttccc caaggcattg tcttgcggca
    10381 tttataccgg caacaagacc tgaagaagca gattcgacat aaccctccac acccgtgatc
    10441 tgtccggcaa agtacaattc aggtcttgat ctcatcctgt aagtcggatc gatgagtccg
    10501 ggcgaatcga tataaatatt cctgtgcatg acgccatatc tgatgaattc cgcattttca
    10561 agcccgggta tcaggcggaa aactcttttc tgttccggcc agcgcagatg tgtctggaac
    10621 ccgaccatgt tatagagtgt gcctgccttg ttatcctgcc ttaactgcac caccgcatag
    10681 ggttctttcc cggtggcggg atcgattatt ccgacgggtt tcagcggtcc gaaccgcagg
    10741 gtgtcccgcc cgtttgcagc catactttcc accggcatgc atccgctgaa gagtatcatt
    10801 ttttcaaaat cccttatagg gaccgtttcc gcgttgacaa gctcgttcca gaacaactcg
    10861 tactgctccc tgtccatcgg acagtttata tagtcgtcgg tacctttccc gtagcgtgaa
    10921 gcgcgaaaag cctttgtcat atctattgac tcatatgtga ctatcggtgc ggcggcgtca
    10981 taaaaataaa ggctgtccct gcccgtgacc ttcattatat gctccgcaaa ggcatctgac
    11041 gtcaacggtc cggtcgcaac gacagtgatg ccgtcttccg gcaggctcgt gacttcttca
    11101 ctgtacacaa taacattggg atggtctttc actgccttag tcacagcttt ggagaaccct
    11161 tctctgtcga ctgccagagc ccctccggcc gggagcctcg tttcatccgc acatttcatt
    11221 ataatggagt caagtctcct catttcttcc tttagcagtc ccactgcatt cgtcaggctg
    11281 tcggatcgta aagaattgct gcagaccagt tccgcaaaaa aatccatttt atgcgcaggc
    11341 gtgtatttct tcggcttcat ctcgaaaagc cttacctgca ctcccctttt tgccgcctgc
    11401 catgcggctt cgcatcctgc aagtcccgcg cctataacat tgatgtatct ttcagccaaa
    11461 tgcgaggccc tcctgtttca gaaaccagta tcattctctg gaatacttgt tttccgccca
    11521 tattgcgata tctactgcac cgttgccgtc agcctgttcc gcctgcatca ttcgtcactg
    11581 ctctcagcct cttccggctg ctttttcctg tccggacttt ttgtgaatcc gcatttttca
    11641 ttgctgcata caagagtcac ttttctgccg gagtacttct ttaccatgaa gtttccgcat
    11701 ttggggcaca tctcgcccga aggcatatcc catgacatga atttgcattc gggatagttt
    11761 tcacacccga gatagctctt ttttgttttc gtctttttta tcagtacctt gccgccgcag
    11821 agcgggcaat tgactccggc ctcttcaagt atcggctttg cgttcctgca ctcggggaaa
    11881 cccggacagg ccaggaattt accgtagcgt ccggttttga tcaccatgtt cctgccgcat
    11941 ttttcacata tcacatcggt cacttcatca ggaatgtcta tattttcaat cctggtttcc
    12001 gcatctttca gcgttttggc aaagggttcg tagaactcct tcatgagttc gacccacttc
    12061 ttggcgccct cctcgacatc atcgagtttt tgctccaact gggccgtaaa ctggatatca
    12121 acgatgtctt tgaagttgtt tttcataagc tcgttgacta tcctccccaa ttccgtcggg
    12181 accaggaact tcttatcctt gaccacgtat ccccttgcaa ggatcgtcgt aattatcggc
    12241 gcatatgtac tcggtctccc gatgcctttt tcctcaaggg cttttacaag cgtagcctct
    12301 gtatatctca taggaggctg cgttaaatgc tgcttgtctt cgatcttttt cagcatcagc
    12361 atttcatcct gttccagatc aggtatggat acttcgtttt ctttggtatc atcatccctg
    12421 ccttcggtat aaactgtaag gtacccgtcg aatctcattt tcgacccgtt ggctttgaaa
    12481 agcaggtctc ctgcaagtat atcagctgca atggtatcat aggcagccga tgccatctgg
    12541 cttgcgatga acctggacca tatcaggctg tacagcttgt actggtcatt ggtcagcgac
    12601 cctttgacgc ttgcgggatc aagttccaca tatgtcggtc ttatggcttc atgggcatcc
    12661 tgggacgccg aacggttttt gtatacccgc ggctcattcg gcaggtattt ctccccgtag
    12721 ctggcatgta tatactctct tgccgcctgc tgcgcttcag ccgatatcct ggttgaatcg
    12781 gtacggatat aagtaacaag accgactgaa ccatgtcctt tgacttcaag gccttcatac
    12841 agctgctggg ctatcatcat cgttttcttt gtcgggaaac ccagtttcct cgacgcttcc
    12901 tgctgcaaag tgcttgtcgt gaacggagca ggcggagttt tcttcttttc gccgtatttt
    12961 accttatgga cgatatattc cttcccttca agctgcttaa gtatctccct tacctgttcc
    13021 tcatccttca gttctatctt ctggtccatt ttgccgaaga attttgcttc gaatgtgcgg
    13081 gaggatttct ttttatgaag caatgccgtg atcgtccagt attcttccgg tatgaaattc
    13141 tctatctctt cctccctgtc acataccagc cttgcggcga ccgactggac acgtcccgcg
    13201 ctcaggcctt ttctcacctt cctccacagc aggggactta tcttgtagcc tactatcctg
    13261 tccaggacgc gccttgcctg ctgggcattg accaggtcca tgtttatttt tcttggctgt
    13321 tttatggcat ttcggactgc gttttttgtt atctcattga acgtgaccct gcattcctca
    13381 ttttcgtcta tattgagcaa ctgtgcgaga tgccaggata tggcctctcc ttcacggtca
    13441 gggtcggtag caagtagtat ttttttcgag ttccttgctt ctttcctcag tttgctgatt
    13501 atgtcgcctt tgccccggat ggtgatgtat tttggttcaa agtcattttc tatatccacg
    13561 cccatctggc ttttaggcaa gtcccttaca tgtcctaccg atgcaactat tttataatcc
    13621 tttcccagga atttgcttat ggtattggcc tttgcaggcg actcaactat tacaaggttg
    13681 tttgccatca tgtcctcctg aaggttgttt atgataactt taaagcttat cgttctatat
    13741 tgattaaatt caactcatat attaccacat tttacgcttg tgtctacagt aatgataatc
    13801 attttattca acgagcctgt aatatttgcc cggcaattgt tccacgaaac cgctcagttc
    13861 cagcatcaac aggatcgaag atgccatctg gacactgatc ccgcagtccc tgcatatgac
    13921 gtccagatgg acaggaccat tgagcagccg ctgagctata gttttttcat ctctgttcag
    13981 tttcattaca gcaagttttt tagcgctgct atatgtattt ttagaatcat gtcttatatt
    14041 caattcatca agtatgtcgc ccactcctgt tacgatcttc gcgccgtcgc ggatcagcct
    14101 gttggtgcct gcgctgttgt aagagtttat attgcccggc actgcaaaaa cgtctcttcc
    14161 ctgatccagg gcatattctg cggtaataag ggagccgctt ttttcaccgg cttcgactat
    14221 ggtcactcca agcgatatac cgcttattat gcggtttctc gcaggaaaat tgtaccgtgc
    14281 gggttccgta cccggaagat attccgatat tacggcgcca tgccgtatta tttcctccat
    14341 aagtccacgg ttttcaggcg ggtatgcaat atcgataccg caccccagca ccgctatcgt
    14401 cctgcctcct gcttccagtg ctcctttgtg cgcctgcgag tcgatccccc tggccatgcc
    14461 gctgacaacc gtgactccgt gcctggccag atcccccgct aaacgttttg ccatgtcgag
    14521 accgtaccat gtcgccctcc tggaaccgac cacggccata caggtctcat ccggttccgg
    14581 tttgcccctg cagtaaagga tcagcggcgg gtcagcgatg tgcttgagca gttcgggata
    14641 tgtcctgtcg tatattgtta ttacatcggc atcgcacttt ttgacggatt cgaatgccct
    14701 ttcggtttcc ttccttgccg ttttgtcaat aagatgcgtg atcagttttt tagagcagag
    14761 cccggaagca gccaaatcag cttcaccggc ttcccagacc agagcggggt ccctgaaata
    14821 ttgcagaagc tgatatttct tcccgggact tgcattgacc atggaactca gccaaatcca
    14881 gtatttcaga ttgctcattg cctcatcccc cacgtcctgc tgtaggattt tatccattaa
    14941 gtcgattttc atactgctgc tgataccgac cggtttgagt tcagcaactg cgttcatata
    15001 ttccatgctt ttctgtcaag gctcctgtac tgtatcgcct ctgtcagatg tacaggtttg
    15061 atatcttcgc tcatgtccat atcggcgatg gtcctggcca ctttgagtat cctgctgtgg
    15121 gcccgtgcgc tcattccgag cctgtcgaac acatcccgaa ggatacttct gcatttttta
    15181 tcgagcctgc agaattttgc aaacattgca ggctgaagct gggagtttga gtaaatgttc
    15241 agccctttat acctatcaag ctggattttt ctggtccttt ccactctttc cctgatggca
    15301 agcgaactct cgacgttttt atcgctttcc agttccgcat atttgacagg cgaaacctct
    15361 atgtggatat ccagcctgtc aagcagtggc ccggataacc tgctgagata ttgctgtatc
    15421 tgtttgggcg tgcatgtgca ctttccgggc tgatccagga aatacccgca cctgcatgga
    15481 tttgccgcac atatcagtgt ggtccttgcc ggataggtga ggctcgcgtt cacacgggaa
    15541 atggtaatga caccgtcctc cagcggctgt ctcagggcct ccagggcttt cttgctgaac
    15601 tcgggaagtt cgtcaaggaa caatacgccg taatgagcga ggcttatttc gccgggtttt
    15661 ggaatggaac cgccccctac aagtcccgta tccgagatac tgtggtgcgg gctcctgaac
    15721 ggtctcgtgt tcacaaggga tactccaggg ggtagtattc ccgctatact atgtattttc
    15781 gttacctcca gcgcttcctc aaaagtcatc gacggcagta tagtcggaag cctccttgcc
    15841 agcatcgttt tgccgcatcc gggagctcca agcataaggc agttgtgtgc tcccgacgca
    15901 gccacttcca gtgccctctt tacattctcc tgtcccttca catcggcaaa gtcgactgta
    15961 tcagaataaa ccggcacgac ttcgcctccg gtatcctcaa agtattttat ctctatctct
    16021 ccgttaataa ttgccgcagt ttccctgaga tccttcacgg gtacgatatg gagttctttt
    16081 acgaccgaag cttcccttgc gttcgcttca ggaaggaata aaaaacggaa accgttccgg
    16141 tgtgcgcaga cagccatggg caggactcct tttacaggct ttataccccc gtcaagggca
    16201 agctctccga taaacatcga tttactggcg gcagactggt caatgtaacc cgatgatgtc
    16261 aggatgccca tggcaatggc aagatcgaat gatgaacctt ctttctttat gttcgcgggg
    16321 gccatgttca gcgtgatctt tttcagcggg aactcgtatc ctgagttctt cacggcagac
    16381 cggacacgct cccgtgcttc ccgtacggcc gtgtccccaa gcccgactat ttcgaacgca
    16441 ggcagaccgt tgctgatatc tgtctctatt tcgactatat atccgtcgat ccccaaaaca
    16501 ccgcagctta tgatctttga gtacatatta ctacccatag ctagaattag tttctatgtt
    16561 atacagtaat ttccatcaca atattactat atccgcatga ttttgtacat acccaaatta
    16621 tcatctgaca cttttttata catgatgaaa catcacgacc gctataagac aatatacagg
    16681 ctgaattcgc agagaagttg caaagaatgt aaagaatccg atagtatcgg aaatacatcc
    16741 ccggatcggg aaaatcaaat catataatac ctgataaggc aaaagaaatg atgcagattg
    16801 atacgaaagc aactcgaaaa ataatcaggc aacaaaagtt tcaaatatgg cacagcagcc
    16861 tgccgatctg cggattctgt cggcgttgcc gttgtatggc tttgagttgg cctgctcgct
    16921 aattattctt atttcccggc aggttctcag tctcatgtat atttcccagc gggttccgca
    16981 tcccgtgaac atttcccggt tgcttattta tggtcgtcct gtccgttttc ttcctgctgt
    17041 tcgttttcct gaagcttctc attttctttc agcagtttat gtaactcttc catgaatgtg
    17101 tttatgtcct tgaactgcct gtagactgaa gcgaacctga catatgcgac ttcgtcgata
    17161 tccttaagcc ttgccatgac catttcgccg atctcttttg tggttatctc cctcttgaga
    17221 gaattcagta tctggagttc gatctcatca gccagttttt caagagtgtc caccgagacc
    17281 ggcctcttct cacaggctct cagcagtccg ttgatgagct tttcacggtc aaacggctgc
    17341 cttgtcttgt cctttttgat taccatcagc ggtatgctct ccacttt
//
LOCUS       DAOSCC010000020        17331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2270_length_17331_cov_3.710928, whole genome shotgun sequence.
ACCESSION   DAOSCC010000020 DAOSCC010000000
VERSION     DAOSCC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17331
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2270_length_17331_cov_3.710928"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLG72_02185"
     CDS             <1..836
                     /locus_tag="PLG72_02185"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79645.1"
                     /translation="LWVANQVFYRALLAGQEVRRKRKTLTGEQIYRRVGRGSSPVLAL
                     LKREWRLMIRTPLYVLNGLTGSIAGPLIIVLMFAVRGSDPELARLAESLKDPEVIPYA
                     ALGGLGIMLFTAGMNLVASTALSREGKTIWVAKMIPVTARQQVDAKFLCSYIVSAAGV
                     LATSLIMALLLRIPILWVVLPAIAGLIGSVPMVALNLLIDVFHPKLVWNSEQEAMKQN
                     MNAGLGMLVSFVVILIMGACAAGALLLGLPVTAAYAAVAVVSVPALHAVAEKKYSEIE
                     A"
     gene            868..1446
                     /locus_tag="PLG72_02190"
     CDS             868..1446
                     /locus_tag="PLG72_02190"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ79646.1"
                     /translation="MSKQIWKPSTLLNPVPVVMVTCADTSGKPNIITVAWAGTVNSEP
                     PMVSISVRKERYSHNLIKENGQFAINLATEKLAFATDICGVKSGRDVDKFQLTGLTPV
                     KASAIDVPIIKESPVALECVVKNVLELGSHDMFIAEVVAVQADESLLNEKGKLVLEKA
                     DLICYSHGKYWTLGRELGFFGFSVAKKKNIVR"
     gene            complement(1502..2749)
                     /locus_tag="PLG72_02195"
     CDS             complement(1502..2749)
                     /locus_tag="PLG72_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ79647.1"
                     /translation="MSELRGAAIFGQSGGPTSVINASACGVFQEALRQDCITAVYGAA
                     HGIKGVLEEKFYDIGQEDPYELELLKTTPSSALGSVRYKLKNAEDDDTDYKRLLEVFK
                     KYNIRYFFYNGGNDSMDTCNKISKYMQSVGYECRVMGVPKTIDNDLWGTDHCPGYGSA
                     AKYIATSIMEVYHDARVYDKGQITVLEIMGRNAGWLTASAALAANAGFGPDLIYLPEL
                     PFDMDKFIEETMDVYRRQNNVIIAVSEGIKDKDGMYISEYGSDLAKSKDAFGHAQLGG
                     LANTLANIVKEKTGAKVRGIEFSLLQRCAAHCGSLTDVNEAYMAGQMAVRYAVEGKTD
                     YMVAFERVAGPEYKCVTKLINLTDVANTEKKIPREWINEAGNGLNQQFIDYALPLIQG
                     ESHPPLENGLPRFAKLKKVLAAK"
     gene            3047..4006
                     /locus_tag="PLG72_02200"
     CDS             3047..4006
                     /locus_tag="PLG72_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ79648.1"
                     /translation="MNYRTLGKSDVKVSEIILGCWAMGGGYFGDVADRDSIEAIRASF
                     ELGVNTFDTAEVYGRGHSERVLGKALEGIDREKCVISSKVFKHNMRHDLLIRACEQSL
                     RNLKTDYLDIYFLHYPVDNVPIGESMEAMLELKERGLIRAIGLSNFSLEQQKEAMKYG
                     PVDVIQPCYSLLWRYHDKDLLPFCIENDIGVVPYSPLAQGLLTGKYKKTDVFTDGRSR
                     AALFQPENYRRCLDVTEVVKAVSARHGKTPAQGAINWLLMTPGITAPIVGAKNAEQAR
                     ENIGAAGWRFTDEEYAEIDEASRKFTDTLPKYALFFSTETVKE"
     gene            4043..4912
                     /locus_tag="PLG72_02205"
     CDS             4043..4912
                     /locus_tag="PLG72_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ79649.1"
                     /translation="MSLVKVNELLAHATSKRYGVAAINIFNYETIKWAVQAAESEHAP
                     IIIQFYPGFENHLPCRYVAAIAKDMAERASVPVAVHLDHSRDYETVAAGIRDGFPSVM
                     VDGSALPFEENVRLTAEVVRIASIFGVDVEAELGHVGSGANLEDFADSGRYTSVEQAV
                     EFVARTGCNSLAVAVGNAHGVYIREPKLDMDRIRALRRALDIPLVLHGCSDIPDDQIR
                     ESVELGISKFNIATEYDRAIYRVFAGTIDTDEEKGSFFNLIKQSEETIIDFVRRKIRL
                     LNPNRYTLQPAEI"
     gene            4962..5939
                     /locus_tag="PLG72_02210"
     CDS             4962..5939
                     /locus_tag="PLG72_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ79650.1"
                     /translation="MFDIVGLDMPCMDLLVNVDNFPKPNGMALVNSLSWQGGNKVSTG
                     MVAAARLGAMCAIMGAVGDDSYGRFCLWDFERHGIDTSGMLIRKDATTSLSIVISDRE
                     TRGRSFVFHKGSAEPCSIDEIDHCKLEQARFFFVSTLDETAIHCCRTAKQAGAEVFVD
                     ADTYSETMMDNMHLIDWFIASEFVYEALFDNSDYEANCRKILENGPKAVVFTLGGKGC
                     VGISSEGFFRLEAYPVDVVDTTGAGDVFHGAFLAGLLQNWSIRKCADFSNAVAAIKCT
                     RQGGRAGIPDMATALRFMETGEIDCTDIDKRVELYERGISNVGTVWTQV"
     gene            5911..7317
                     /locus_tag="PLG72_02215"
     CDS             5911..7317
                     /locus_tag="PLG72_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOJ79651.1"
                     /translation="MSELYGHRYEAKITLGVAPTKRSFLSMEEAKRQKDRFMAVIRSI
                     RPDIVKIVDIDDICENGIMYRTTDTDKVIGKLRRAGIDALFIPHCDFGEEQVSAAVAA
                     ALKVPTLIWGPRDERPNTDEARGRDTQCGMFACTKVLLRYGVKFSYIFNCATESREFS
                     EGYTNFIRTAAVLKALKGLRIAKLGERPVPFLSVTANEGDLMTKFGITTVPVRLNDVV
                     EDAVAIERENSSFFSSYVADLKSRMDCSATPEDMLRRSAALKLAVKKRMLENGCTVGA
                     MECWPARDLFGVMICSVLGEMADDGYPISCETDVNGAVTMAILRACSLDEGSVFLADL
                     TIRDPENDNAELLWHCGCFPYSLKDPSSGAKLVNGVESFRLRKGNLTVCRFDDLNGDH
                     YLFAGEGKAISGPETNGTYVYLETENWKRWEEKFIFGPYIHLVGCVYGSYFPALREAA
                     RYLDVKFDRVNDGGPFSL"
     gene            8109..8987
                     /gene="nifH"
                     /locus_tag="PLG72_02220"
     CDS             8109..8987
                     /gene="nifH"
                     /locus_tag="PLG72_02220"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HOJ79652.1"
                     /translation="MAKKIKQIAIYGKGGIGKSTTTSNLSAALSVAGFRVMQFGCDPK
                     ADSTSTLRNGSYIPTVLDTLREKGKVNAYDVIYEGFNGIYCVEAGGPAPGVGCAGRGI
                     ITAVELFRQQRIFEELDLDYVIYDVLGDVVCGGFAVPIREGIAQHVFTVSSGDFMSIY
                     ASNNLFKGIKKYSNSGGALLGGIIANSINHGYSREIIDDFAARTKTQIMQYVPRSITV
                     TQSELQGKTTIEAAPDSQQAQIYRQLAERIISHEESRVPSPLELDELRNWAAGWSDKL
                     LELESGVVTGGIQAGI"
     gene            9100..9402
                     /locus_tag="PLG72_02225"
                     /pseudo
     CDS             9100..9402
                     /locus_tag="PLG72_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
     gene            9421..10278
                     /locus_tag="PLG72_02230"
     CDS             9421..10278
                     /locus_tag="PLG72_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ79653.1"
                     /translation="MRRPPFVGYKRREVIDDHPCFSEKAHNRSGRLHLPVSPACNIRC
                     RFCKRARNTDEVRPGVAAGLLRPEEAVDTVRRALQLCPQIKVVGIAGPGDTLATDHAL
                     ETFRLVHAAWPDLIKCLSTNGLDLPGKARELWDAGVRTVTVTVNAVDPGILGKIVSWV
                     KDGRDLISAQLQGIRECADIGMAVKVNTVLIPDINDTHVADIAKAVSEAGAVRQNIIP
                     LIPQSEFSDMQPPTCGEIEKARQEAGKYIEQFRHCKHCRADACGIPGVSDLTKELYQG
                     RELETFSHG"
     assembly_gap    10281..10380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10381..11202
                     /gene="cysK"
                     /locus_tag="PLG72_02235"
     CDS             <10381..11202
                     /gene="cysK"
                     /locus_tag="PLG72_02235"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ79654.1"
                     /translation="EYFNPLGSVKDRIAYAMIRDAEESGRLTKGMTIIEPTSGNTGIG
                     IAFVAAALGYKVILTMPETMSVERRNLVKALGADVVLTPGAEGMKGAVRRAEELNAQI
                     PGSIILQQFENPANPAIHYSTTAEEIWRDTDGKIDIFIAGVGTGGTVTGVGKNLKEKD
                     PGIKVIAVEPFESPVLSGGQPGPHRIQGIGAGFIPLVYDPDVVDEVITVRGDEAIETS
                     RELGRSEGLLVGISSGAAAFAAAAVGKRAENRGKTIVAVLPDTGERYLSTILYQY"
     gene            11222..12529
                     /locus_tag="PLG72_02240"
     CDS             11222..12529
                     /locus_tag="PLG72_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOJ79655.1"
                     /translation="MPDFIESPRTACALAGALSTISCLPGVIPIIHTTLGCGGNLANA
                     VAFGSGYLGGGYCSGAQAPSSGVTETEIVFGGAERLHEQIRNTLELIDGKLYIVATGC
                     MTEMIGDDTDGVANGFLDEGEPVIAVSTPSFKGDSYHGYEIVLDAIFNRYLPRAEHKN
                     PKLVNIFGLIPGYDPFFRGDLEEMERLLTSLGLEVNTFFTPSQTFENITSAPTAALNI
                     VFSRVWGKTLAGRFEQRHGTPYIICDLPIGPEATDGFLRRIAEWFGLDAGHVIERENR
                     IFYGYFERAADAFCDQDLKFYAITVTNSNYAIPLARFLSRELGWVVADSFVTDKLTDE
                     QKAALSGDYPLQFRTGVQEIARAISKNHPNSRGRRYFDDITPLYIIGSSLEKRTANAR
                     GAMHLSVSFPVYDRLITDRGYAGYRGGLHLFEDLTGRIMSLKG"
     gene            12534..>13168
                     /locus_tag="PLG72_02245"
     CDS             12534..>13168
                     /locus_tag="PLG72_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOJ79656.1"
                     /translation="MGFYEQSVPPVRDTRLKIGDSYHGNACGLVNCGRDGCLLQKNRG
                     FWQANACQMALTLMMAATVENAAIVMHAPGGCGALLHSLSVQSAKGKARRGKEPKAVP
                     WLSTNLTEADVIGGGEKKLLETIKYADREFRPEIIFVVVTCAPSIIGDDAEEVVRQAR
                     PQVSADVVSLHCPGFRSRVVASAYDAFYHGILRGLELSPKPWVDFRPLPQGS"
     assembly_gap    13169..13268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13269..14206
                     /locus_tag="PLG72_02250"
     CDS             <13269..14206
                     /locus_tag="PLG72_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOJ79657.1"
                     /translation="LELSPKPWVDFRPLPQGSPEAKLAEKNYQFERSRTVNVWNATSI
                     GPADEGEVRRLLTALGLKVRFFAEYSSRDELRMVSEAALNVSMCNVHDDYMLKFLEEK
                     FGTPYVIAGMPIGFRHTRQWLVSVARHFGLEKQANALADYEENLAREAIGPFVEQVRG
                     KKVLICGGVVRVGAEATMLAELGLDVLGIRAYHYDDGAEPVYEDVVRNLPDTPVAVSN
                     QLFELTHQIKTLKPDIVISHNGTQGYIAKLGVPSVQLWNVDSAFFGYTGIYQILRRIV
                     FELKNTSYRDRLSKHVKLPYKPAYFEGDTFKYIKD"
     gene            14296..15435
                     /locus_tag="PLG72_02255"
     CDS             14296..15435
                     /locus_tag="PLG72_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ79658.1"
                     /translation="MLILTLVFSITGCSGKNDAGSAKDASPAAGQTGTGGESQTDCCD
                     DADATASADGQTGKGSGAGEQTIDKEAAFLDEPASKRTIHIGYDGGLCQAAIPIAHHK
                     GFFEAEGLKTELTRTGGTHDNVRDALAAGKIDTTAGMIAGWLKPITNGIDLKFTVGLH
                     TGCASAFVLADSPIRSFADAKGKTVAINGGIGGVYHNIGYRFIAHDGFAPEDFIWRDF
                     PADQTLLILQKGEADVAVFSDQLAEKWVQDGTLRRIRSLHEDEDFRNESCCVLGISGS
                     FIAENPVASEKISRAIYKASLWIGESDANKEEAVQILLDNDYISGTKEYALQLMKLFR
                     WGLPNELTEATLDLSIKDYQRLGVISDSIDPAELKAQIWVPLDIE"
     gene            15493..16515
                     /locus_tag="PLG72_02260"
     CDS             15493..16515
                     /locus_tag="PLG72_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ79659.1"
                     /translation="MKLTAWELHRLESSPKSKLRRTFERILTILPLLLVMAHMAEYKL
                     MPDFPKYVANRIAYPVSDTYTVFLGILAFALLIYLILSFFSDRKYERLVHSSPLYCVI
                     AIILSIYDIATLKTGILQLPYFPWADRILLAIIEDRKMLLDCVTNSLILLFAGYLAGA
                     AAGLFCGVGAGWSKKIRYWIMPLLKLLGPIPSTTWISIILVVASSLFAGSVFMIALGV
                     WYPVTMAALNGVANVNRSNFEVAKTFGANRFELVFKVAIPAAMPSIFNGLTQGMGIAC
                     TALMVAEMLGVESGLGWYINWQRGWAEFAKMYAAVIIICLTFTAVNFVLTLIKKRVLR
                     WKEEYV"
     gene            16512..17291
                     /locus_tag="PLG72_02265"
     CDS             16512..17291
                     /locus_tag="PLG72_02265"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79660.1"
                     /translation="MSGQVEIRNLSKVFARPGSDDTLQALDNISLDIRPGEFVALLGA
                     SGCGKSTLLRIIAGLEIPTKGEVLFNGGRICGTSPKRGLVFQEHSLFAWLTVRQNIEF
                     ALKTSRQYQDRKCEIDRWLELAGLAEFAGNYPHQLSGGMRQRAALVRALAVSPDVLLL
                     DEPLGALDSFTRMSLQDELVRLWQERGNTMVMVTHDIDEAIYLSQRIVIMSPRPGRIS
                     RIIEVRRSYPRDRASGDFTVLRNEILRFMNFAHEQQYDYVI"
BASE COUNT         4202 a         4284 c         5001 g         3644 t
ORIGIN      
        1 tgctgtgggt ggcaaaccag gtgttttaca gggcactgct cgccggccag gaagtgaggc
       61 ggaagagaaa gacgctcacc ggcgaacaga tctacaggcg tgttggcagg ggcagcagtc
      121 cagtgctggc tctgctgaag cgtgaatgga gactgatgat acgcacgccc ctgtacgtgc
      181 tcaatggcct tactggcagc atagcaggtc cgcttattat agtgctgatg ttcgccgtgc
      241 ggggaagcga ccctgaactt gccaggctgg cagaaagcct gaaagatccg gaagttatac
      301 cctatgccgc gcttggcggg cttgggataa tgctgtttac tgcaggcatg aacctggtcg
      361 cgtcaactgc cttgtcaaga gaaggcaaaa ccatatgggt cgccaaaatg atacctgtga
      421 ccgcaaggca gcaagttgac gccaagttcc tgtgcagcta tatcgtatct gccgcaggcg
      481 tgctggcaac cagccttatc atggcattgc tgctgaggat acccatattg tgggtagtgt
      541 tgccggccat agccgggctt atcgggtccg tgccgatggt ggcgctgaac ttgctgatcg
      601 atgtattcca cccgaaactc gtatggaaca gcgaacagga agccatgaag caaaatatga
      661 acgcaggact tgggatgctc gtctcatttg tggtaatact cataatgggt gcttgcgcag
      721 ccggagcatt gctcctggga cttcccgtaa cagcagctta cgcggcggta gccgttgtat
      781 cggttccggc gctccatgcc gtagccgaaa agaaatacag tgagatcgaa gcctgatatc
      841 tgtactatca actgggaggg ataaaaattg tcgaaacaga tctggaaacc ttctacatta
      901 ctgaaccctg tgccggtggt gatggtaaca tgtgccgaca cgtcagggaa gcccaacata
      961 atcacggttg catgggcagg aacggtaaat tccgagccgc cgatggtatc catatccgta
     1021 aggaaggaaa ggtattccca taatctcata aaagaaaatg gacagttcgc aatcaacctg
     1081 gcaacggaaa agctggcttt tgccaccgat atatgcggcg taaagtccgg ccgcgacgtc
     1141 gacaagttcc aactgaccgg cctgactcct gtaaaagcct cggccatcga tgtgccgata
     1201 ataaaggaaa gccctgtcgc attggaatgc gtggtgaaaa acgtgctgga gcttggcagc
     1261 cacgacatgt tcattgccga ggtggtcgct gtccaggcgg acgaatcgct gctcaatgaa
     1321 aaggggaagc tggtccttga aaaagccgat ctcatttgct actcccacgg caaatactgg
     1381 acactgggca gggaactggg gttcttcgga ttctccgtag caaaaaagaa aaatatagtc
     1441 agatgaaaaa cggagtgccg taaggcactc cgttgtgttt cggtcgatta tcgggccgtg
     1501 tttattttgc cgcgaggacc tttttgagct ttgcgaacct gggcaggccg ttctccagcg
     1561 gaggatggga ctcgccctgg atcaggggca gtgcgtagtc gatgaactgc tggttcagcc
     1621 cgttgccggc ctcgttgatc cactcgcgcg ggatcttctt ttcggtattt gcaacgtctg
     1681 tcaggttgat aagcttggtt acgcacttgt actcaggacc tgcgactctt tcgaaggcca
     1741 ccatgtagtc ggtcttgcct tcgacggcat atctgaccgc catctggccg gccatgtatg
     1801 cttcattcac gtctgtcagc gatccgcagt gggcagcgca cctctgcagg aggctgaact
     1861 caatgccgcg cactttagcg ccggtctttt ccttcacgat gtttgcgagt gtatttgcca
     1921 gtccgcccag ctgtgcatgt ccaaatgcat ccttcgactt tgcaaggtcg gagccgtatt
     1981 ctgagatgta catgccgtcc ttatccttga taccttcgga aacggctata ataacgttgt
     2041 tctgccttct gtacacatcc atcgtctctt cgatgaattt gtccatgtca aacggcagtt
     2101 ccggcagata aataagatcg ggcccaaatc cggcgtttgc tgcgagagcg gccgaagctg
     2161 tcagccagcc ggcgttcctt cccattatct cgagcactgt gatctggcct ttgtcgtaaa
     2221 cgcgtgcgtc atgataaact tccattatcg aagttgcgat gtacttggcg gcactgccgt
     2281 agccggggca gtggtccgtt ccccagaggt cgttgtctat ggtcttggga acgcccatca
     2341 cacggcattc ataaccgacc gactgcatgt acttgcttat cttgttgcag gtgtccatgg
     2401 agtcgttgcc gccgttatag aagaaatacc tgatattgta ctttttgaat acctcaagca
     2461 gcctcttgta atcggtatcg tcatcttcgg catttttaag cttatagcga accgatccca
     2521 gcgccgagga aggtgtagtc ttcaaaagtt ccagttcata cggatcttcc tggcctatat
     2581 cataaaactt ctcttcgagc acacctttga tgccatgtgc cgcaccgtaa accgcggtga
     2641 tgcagtcctg cctgagcgct tcctggaaaa cgccgcaggc actggcgttg ataactgaag
     2701 tcggaccgcc cgactgtccg aatatagctg cacctcttag ttcgctcata atcacacctc
     2761 ctgataaaat caagataaaa tatagcataa tatgtaccaa aatgcaatta aagatagata
     2821 tatttttaac ataatcgact gcgggctggt ttcctgcata tcggaccgtt ttgaatgcaa
     2881 ttgcttgggc tttatgcgtg ggtttctgcc aatggtttga ttgacatgcc ggctcgtgga
     2941 aactacaatg gtaataggag ccaaatactg cttcctgcca tggctcagac catgattcgg
     3001 gcacttgcag cagatctgtt atcataaaga gggaagaagg ttgcgtatga actacagaac
     3061 gctggggaaa agtgatgtga aggtatccga gatcatactt ggatgctggg caatgggcgg
     3121 cggctatttc ggcgatgtag ccgaccggga ctcgatagaa gcgatcaggg catcgtttga
     3181 actgggtgtc aacacgttcg atacggccga agtatacgga cgcggacatt ccgaaagggt
     3241 ccttgggaaa gcccttgaag ggatcgacag agaaaagtgc gtcataagct ccaaggtctt
     3301 caagcacaat atgaggcacg acctgcttat cagggcctgc gagcaaagcc tcaggaatct
     3361 gaagaccgac tacctggata tctatttcct gcattatccc gtcgacaatg tgcccatcgg
     3421 cgagtcgatg gaggcaatgc ttgagcttaa ggaaagaggg ctgatcaggg ccataggcct
     3481 ttcgaatttc agcctggaac agcagaagga agcgatgaaa tacgggcccg tggatgtgat
     3541 ccagccgtgc tacagcctgc tgtggcgtta ccacgacaag gacctcctgc cgttctgcat
     3601 cgaaaacgat ataggcgtcg ttccatacag tccgctggca caagggctcc tgacaggcaa
     3661 gtataaaaag acggatgtct ttacagacgg ccgcagccgc gcagcgctgt tccagcctga
     3721 aaactacagg cgctgcctcg atgtgaccga agtggtgaag gctgtttcgg caaggcatgg
     3781 caagacgcct gcccagggcg ctatcaactg gctcctcatg acgcctggca tcaccgctcc
     3841 gatcgtcggt gcaaagaacg cggagcaggc cagggagaac atcggagccg ccggatggag
     3901 attcaccgac gaggaatacg cggaaatcga cgaagccagc cggaaattca cagacacact
     3961 gccgaaatat gccctgtttt tctccactga aacagtaaaa gagtgaaata tatcatatac
     4021 aaaaagaatg agaggaagcg gcatgtcact ggtaaaagtc aacgaactgc tggcgcacgc
     4081 aacatcgaaa aggtacggcg ttgccgcgat caacattttc aattacgaaa ccatcaaatg
     4141 ggccgtccag gctgccgaat cggaacatgc cccgatcatc atacaattct accccgggtt
     4201 tgaaaaccat ttgccgtgcc ggtatgtcgc agccattgca aaagatatgg ccgaaagggc
     4261 aagcgtaccg gtggcggtgc acctggacca ttccagggat tatgagacgg ttgccgccgg
     4321 gataagggac ggtttcccgt ccgtcatggt ggatggttcc gcgctgccgt tcgaagaaaa
     4381 tgtgagattg accgcagaag tggtacgcat tgcgtcaatc ttcggagtcg acgtggaagc
     4441 cgagcttggc catgtgggat caggcgcaaa cctggaggac tttgccgatt ccggcaggta
     4501 tacatctgtg gagcaggcag tcgagtttgt cgcaaggacc ggctgcaatt ccctggctgt
     4561 cgctgtcggc aatgcccatg gggtatatat ccgggagcca aagctggaca tggaccggat
     4621 aagggcgctt cgcagggctc tggacatccc gctcgtgcta catggctgtt cggacatccc
     4681 ggatgaccag atccgggaga gcgtggaact gggaataagc aagttcaaca ttgccacgga
     4741 atatgaccgc gcgatatacc gggttttcgc cggaactatc gacacggacg aagaaaaggg
     4801 atcgtttttc aatcttataa aacagtcgga agaaaccatt atcgactttg tccgcaggaa
     4861 gatccgcttg ctgaatccca accggtatac attgcaaccg gccgaaatat gactgctgtt
     4921 atcaagggac gctggattcc aataatgttc aggaggccga tatgttcgat atcgttgggc
     4981 ttgatatgcc ttgtatggat ttactggtta atgtggacaa ctttccgaag ccgaatggaa
     5041 tggcattggt gaacagcctc agctggcagg gcggcaacaa ggtttcgaca gggatggtgg
     5101 ccgctgcgcg tcttggcgcc atgtgtgcca tcatgggtgc cgtcggggac gacagttacg
     5161 gccgcttttg cctctgggac tttgaacgcc atgggataga tacttcagga atgttgatca
     5221 ggaaagacgc cactacttcg ctttccatcg tgatcagcga ccgcgagacc cggggcagga
     5281 gtttcgtgtt ccacaaggga agcgccgaac cgtgctcgat cgatgaaatc gatcactgca
     5341 agcttgagca ggcacggttc ttttttgtct ccactctcga cgaaacagcc atccactgct
     5401 gcaggacggc aaagcaggca ggcgcggaag tattcgtcga tgcggacacg tactccgaaa
     5461 cgatgatgga caatatgcat ctgatcgact ggttcattgc ttctgaattc gtatatgagg
     5521 cgttgttcga caacagtgac tatgaagcga actgcagaaa aatactggag aatgggccga
     5581 aagccgttgt atttacgctg ggcggcaaag gctgcgtcgg gatcagcagc gagggcttct
     5641 tcaggctgga agcttacccg gtggacgtag tcgataccac cggagcgggc gatgtcttcc
     5701 acggcgcatt cctcgcaggg ctgctgcaga actggagcat ccggaaatgc gctgatttct
     5761 ccaacgcagt tgctgccatc aaatgcaccc gccagggcgg ccgggcaggc atacctgaca
     5821 tggcgaccgc actgagattc atggagaccg gggaaataga ctgtacggat atagacaaac
     5881 gtgttgagct gtatgaaagg gggatatcca atgtcggaac tgtatggaca caggtatgaa
     5941 gcaaaaatca cccttggagt cgcaccaacc aaacgcagct ttttgagcat ggaagaggca
     6001 aaacggcaga aggacaggtt catggccgtt attcgcagca tcaggccgga tatcgtgaag
     6061 atcgtggata tcgatgacat ttgcgaaaac gggataatgt acaggacgac ggacacggat
     6121 aaagttattg gcaagttgag aagggcagga atcgatgcgc tgttcatacc ccattgcgat
     6181 ttcggcgagg aacaggtgtc ggctgccgtc gctgctgcgc tgaaggtccc gacacttata
     6241 tggggtccca gggatgaacg gccaaatacg gacgaagctc ggggccgtga cacacaatgc
     6301 ggtatgttcg cctgcaccaa ggtattgctg cgttatggag tcaagttcag ttacatattc
     6361 aactgcgcca ctgaaagcag ggaattcagc gaaggatata ccaacttcat acggacggct
     6421 gcggtcctga aagcgctgaa gggactgcgt attgcaaagt taggcgaaag gccggttcct
     6481 ttccttagcg ttacggcaaa tgaaggagac ctgatgacga aattcggcat aacgactgtg
     6541 ccggtcaggc tcaacgatgt cgtggaagac gccgttgcta tcgaaaggga aaacagcagc
     6601 tttttcagca gttacgtggc ggatctgaaa agcaggatgg actgctcggc cacgccggag
     6661 gatatgctga gacggtctgc ggcgctgaag ctggcagtga aaaagaggat gctcgagaac
     6721 ggctgtaccg ttggagcgat ggaatgctgg cccgcaagag accttttcgg agtcatgata
     6781 tgcagtgtac tgggcgaaat ggctgatgac ggctacccca tctcatgcga gacggacgtg
     6841 aacggcgccg tcaccatggc gatccttcgg gcatgttcac ttgacgaagg atcagtcttc
     6901 ctggcagatc tcacgatccg cgaccctgaa aacgacaatg ccgaactttt gtggcactgc
     6961 ggttgtttcc cgtactcgct gaaagatccg tccagcgggg ctaagctggt gaacggcgtc
     7021 gaatcgttca ggctcaggaa gggaaacctg acagtctgcc ggttcgacga cctgaacggc
     7081 gatcattatc tcttcgccgg cgaggggaaa gccatcagcg ggccggaaac caacggcacg
     7141 tatgtatacc ttgagacgga aaactggaag cggtgggaag agaaattcat attcgggcca
     7201 tatatccacc ttgtcggatg cgtgtacggc agctatttcc cagccctgcg cgaagcggcc
     7261 cgttaccttg acgttaaatt cgaccgtgtc aacgatgggg gacctttcag cctgtaagtg
     7321 cgggtgccgg acacgcaggc tggacagtta taatgagttt gggcgccagg tatggcgatc
     7381 aaacagtaat tgcggattcg attgcctggc gctgcacccg gacgctttcc gttacatact
     7441 cattgaaaaa aatccttccg gagttctgta aatgcagatt acgggaggct ttttgctgtc
     7501 atcagaaaca tcgatggtat cctggaaata tttttttgca aaaacatctt gacaaatgat
     7561 ggttatttct gtaacatata atgcatacta atcatatatg tattataaca atatcactct
     7621 ccaacccagc aaacatcgaa gctaagaaat aaaatgtgag gtcgtcggta tgttgatttc
     7681 agatcatgga aaaaccgaaa cggatgcgat agctgagaca ggcagcatga acagctttat
     7741 gtgctttcgt atgagatgtt gtatggacag ccatatgagc ggctgtatgt gtcgcagcat
     7801 gcgccctcat atgagcggga cctgttgcag cattcgcggt tcggcttttc gacggctctg
     7861 atcaggcata gatggtcttg cctggcgttc ggatgccgaa ttgcatgatc cggacgcttt
     7921 tttcataacc caaaacatac taaacatata tgtaatataa taaactggca gatatccctg
     7981 tccttattcg gcagatatcc atgcttcgct taatactccc gggacggaag tactctgcca
     8041 ggcaaaagca agttttcttc aagaagcaaa atcgttgtga acaacagcaa tgtaaaggag
     8101 aatggaccat ggcaaagaag atcaagcaaa tcgcgattta cgggaaaggc ggcatcggga
     8161 aatcgaccac caccagcaac ctttctgccg cactgtctgt tgcgggcttc agggtcatgc
     8221 agttcggatg cgacccgaag gccgacagta cgagcacgct gcggaacggc tcatacatac
     8281 cgaccgttct cgacacgctc agggaaaaag gcaaggtgaa tgcctatgat gtcatttatg
     8341 aaggtttcaa cggcatctac tgcgtggaag cgggaggtcc cgcaccgggt gtaggctgtg
     8401 ccggtcgtgg gatcatcacg gctgttgaac tgttcaggca gcagaggatc ttcgaagaac
     8461 tggatcttga ctatgtgata tacgacgttc tcggagacgt agtgtgcggc gggttcgccg
     8521 tgccgatacg cgagggcatc gcccagcatg tattcacggt ttcaagcggt gactttatga
     8581 gcatttacgc aagcaacaac ctgttcaagg ggatcaagaa gtactccaac agcggcggcg
     8641 cgctgctcgg cgggataatc gccaattcca tcaatcacgg ttattcccgc gagataatag
     8701 atgatttcgc cgcgaggacg aaaacacaga tcatgcagta tgtccctcgt tcgattaccg
     8761 taacccagtc ggaactgcag gggaagacca ccatcgaagc cgctcctgac agccagcagg
     8821 cgcagatcta ccgacagctt gctgagcgga tcatcagcca tgaagaaagc agggtgccgt
     8881 cgcccctgga acttgacgaa ctccggaact gggcggcagg atggagcgac aagctgctgg
     8941 aactggaaag cggtgtagtt acaggcggca tacaggcagg catataagcg tgcggaaatg
     9001 ggtcgattgc tgccgggagc attggaacgg aagggcaatg aaatcaccct ggccgatgtg
     9061 tgtgatgcct acatgtttat ataaatggga gtggagctta tgaaaatagc atttgcttca
     9121 aacgatggga tatatgtcag ccagcatttc ggacacgctc gaaggttcct gatcgtggag
     9181 atagacgacg tcacccatgc caggaggtat cttgagaccc gcgaaaacga cagcccatgc
     9241 aagtacggcg atcacgacga agagaaattc aacaggagcg tcgaactggt cgccgactgc
     9301 aaagcgctgt tctgcgtcaa ggcggggagc tatgcgcagg taaggctggg caggatggga
     9361 gtgcaggtca tcgagaagca cggttttatc ggggaactgc tcgatgatta tatcaggtac
     9421 ctgagacgtc cgccgtttgt cggatataag cgaagggagg tcatcgacga ccacccgtgc
     9481 ttcagtgaaa aggcgcacaa caggagcgga aggctgcacc tgcctgtcag cccggcgtgc
     9541 aacatccggt gccgcttctg caaaagggcc aggaacaccg acgaggtccg tccgggtgtg
     9601 gcagcagggc tgctaaggcc ggaagaagcc gtagacacgg taagacgtgc gctgcagctc
     9661 tgcccgcaga taaaagtggt cggcattgcc ggaccgggag atacgctggc gacggaccat
     9721 gcgctggaaa cgttcaggct cgtccacgct gcctggccgg acctgatcaa gtgcctgtcg
     9781 accaacggcc tggacctgcc gggcaaggcg cgggaactgt gggacgcagg cgtgaggacg
     9841 gtgacggtca cggtgaatgc tgtcgacccc ggcatactcg gaaaaatcgt gtcatgggta
     9901 aaagacggcc gcgatcttat ctccgcccaa ttgcagggga tacgtgaatg cgctgatatc
     9961 ggcatggcag tcaaggtaaa tacggtgctt atcccagaca tcaacgatac gcatgttgcc
    10021 gacatcgcaa aggcagtcag cgaggctgga gcagtacgcc agaacatcat cccgctgata
    10081 ccgcagagcg agttttccga catgcagccg ccgacctgcg gagagataga gaaagcgcgg
    10141 caggaggcag gaaagtacat agagcagttc cgccactgca agcattgccg ggcggacgcc
    10201 tgcgggatac cgggcgtgtc ggacctgacg aaggagttgt accagggacg tgaacttgag
    10261 acgttcagcc acggatgagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 gaatacttca atccgctcgg cagtgtaaag gaccgcatcg cctacgccat gatcagggat
    10441 gcggaagaaa gcggacggct gacaaaaggg atgacgatta tcgagccgac cagcggcaat
    10501 acaggtatcg gcatagcgtt cgtcgcggct gcgctgggtt ataaagtgat cctcactatg
    10561 cccgaaacga tgagcgtcga gcggcggaac ctcgtcaagg cccttggggc ggacgtcgtc
    10621 ctcactcccg gcgccgaagg gatgaaaggc gctgtccgcc gtgcggagga actcaacgcc
    10681 cagataccgg gcagcataat actgcagcaa ttcgagaatc ccgccaaccc ggcgatccat
    10741 tacagtacga cggcagaaga gatatggcgg gatacggacg gaaagatcga catattcatt
    10801 gccggtgtcg gtacgggcgg gacggtgaca ggcgtcggca aaaacctgaa ggagaaggat
    10861 cccggtataa aagtcatagc tgtcgaacct tttgaatcgc ccgtcctgtc cggaggccag
    10921 ccgggacctc acaggataca gggcatcgga gccggattca taccgcttgt atacgatccg
    10981 gatgttgtcg atgaagtgat aaccgtccgc ggcgatgaag cgatagaaac ctcgcgcgag
    11041 cttggacgct cagaaggcct gctcgtaggg atctcgagcg gagctgcggc attcgcggcg
    11101 gctgccgtcg gaaagcgtgc cgaaaacagg ggaaagacga tagtcgctgt gctgcctgac
    11161 acaggcgagc gttatctttc cacgatactg taccagtatt agtccaaaag ggggagtgaa
    11221 aatgccggac tttatcgaaa gcccgcgtac ggcctgcgct cttgcaggtg cgttgtcgac
    11281 gataagctgt ctgcccgggg tcataccgat catccatacc actctcgggt gcggcggcaa
    11341 cctggccaat gctgtggcgt tcggttcggg ataccttggc ggaggttatt gcagcggcgc
    11401 ccaggctcca agctccggag tgacagagac ggaaatcgtc tttggcggcg ctgaaaggct
    11461 gcacgagcag atacgcaata ctcttgaact catcgacggg aaactgtata tagttgcgac
    11521 aggctgcatg accgagatga tcggcgatga cacggacggc gtggcaaacg gattccttga
    11581 cgaaggagag cccgtgatcg cggtgagcac cccgagtttc aagggcgaca gttaccacgg
    11641 atatgaaatc gtgcttgacg cgatattcaa caggtacctg ccaagagccg aacataaaaa
    11701 cccaaagctt gtcaacattt tcgggctcat accgggatac gacccgttct tccggggcga
    11761 cctggaagag atggaacgac ttctcacatc gctggggctc gaagtgaata cttttttcac
    11821 gccgtcgcag acattcgaaa atataacgtc agcaccgacg gctgcgctga acatcgtttt
    11881 ctcgagggtt tggggcaaaa cgcttgccgg gagattcgaa caacggcacg ggaccccgta
    11941 catcatctgc gatctgccca tcggacctga ggcgacggac ggattcctgc gccggatagc
    12001 ggaatggttc gggctggacg ccgggcatgt catcgagagg gaaaacagga tattctacgg
    12061 ttatttcgag cgagcggctg acgcgttctg cgaccaggat ctcaagttct acgcgataac
    12121 cgtcacgaac tcgaattatg cgatcccgct ggccaggttc ctatcaagag agttgggatg
    12181 ggtcgtggcc gacagcttcg tgacggataa gctgacagac gagcagaagg ctgctctttc
    12241 aggcgattat ccgctgcagt tccgcaccgg cgtccaggag atcgcaaggg ccatcagcaa
    12301 aaaccatccg aacagcaggg gccggcgata ctttgacgat ataacgccgc tctatatcat
    12361 aggcagttcg cttgaaaagc ggacggcgaa cgcgcggggc gcgatgcacc tgtcagtgag
    12421 cttcccggtc tatgacaggc ttattacgga caggggctat gcgggatacc gcggcgggct
    12481 gcacttgttt gaagatctta cgggaaggat catgtcttta aaggggtgag attatggggt
    12541 tttatgagca atctgtacct cccgtgcgcg acacccggct gaaaatagga gacagctatc
    12601 acggcaatgc ctgcggcctg gtcaattgcg gacgcgacgg gtgcctgctt cagaaaaaca
    12661 ggggattttg gcaggcaaac gcctgccaga tggcactgac gcttatgatg gcggcaactg
    12721 tagagaatgc ggcgatcgta atgcatgctc ccggcggctg cggcgcgctg ctgcattcgc
    12781 tgtcggtaca gtcggccaaa gggaaagcgc gccgcggcaa ggagccgaag gccgtaccgt
    12841 ggctgtccac aaaccttacg gaagccgatg tgatcggagg cggggagaaa aaactgctgg
    12901 aaaccatcaa atatgcggac agggagttcc gccccgaaat catattcgtt gtggtgacat
    12961 gtgctccgag cataatcggc gacgatgcgg aagaggtggt acggcaggca cgtccacagg
    13021 tcagtgccga cgttgtgagc ctccactgcc cgggtttcag gagccgtgtg gtggcaagcg
    13081 cgtatgatgc gttctatcac gggatcctgc gcgggcttga actgtccccg aaaccgtggg
    13141 tcgacttccg gccgctgccg cagggatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnngg cttgaactgt ccccgaaacc gtgggtcgac ttccggccgc tgccgcaggg
    13321 atcgccggaa gcaaaactgg ccgagaaaaa ttaccagttc gagagatcgc gcacagtgaa
    13381 cgtgtggaat gctaccagta tcgggccggc cgatgaagga gaggtcaggc gcctgctgac
    13441 ggctttgggg cttaaggtaa ggtttttcgc ggagtattca agccgtgacg aactgcgaat
    13501 ggtttccgaa gcggccctga atgtcagcat gtgcaacgtt cacgacgact atatgctcaa
    13561 gttcctcgaa gagaagttcg gaacgcctta cgtgatcgcc ggcatgccga tagggttcag
    13621 gcacacgaga caatggctcg tatcggtcgc caggcatttc gggcttgaaa aacaggccaa
    13681 cgccctggcg gactacgagg aaaacctcgc cagggaagcg atcgggccgt tcgtggagca
    13741 ggtcaggggc aaaaaagttc tgatctgcgg cggcgtggta cgggtcggcg ctgaagcgac
    13801 gatgctggcg gaacttggac tggatgtcct cggtatccgc gcgtatcact atgacgacgg
    13861 agctgagcct gtttacgagg atgtcgtcag gaacctgccg gatacgccgg tggctgtcag
    13921 caaccagctg tttgaactga cacaccagat caaaacgctg aagcccgaca ttgtcatatc
    13981 acacaacggg acgcagggct acatagcgaa acttggcgtt ccgtccgtcc agctgtggaa
    14041 cgttgacagc gcgttcttcg gctataccgg gatataccag atactgcgcc ggatagtgtt
    14101 cgaactgaaa aacacatctt accgcgacag gctttcgaag cacgtaaaac tgccgtacaa
    14161 accggcttat ttcgaaggcg acactttcaa atatataaag gactgaaagg ggatcggttt
    14221 atgaaactta atgattctgt atttcgactt agacctgttt tacaaggtaa atttgcttcc
    14281 aggcttatat cagccatgct tatcctcaca cttgtgtttt caataacggg ctgctcaggg
    14341 aaaaatgatg ccggcagtgc aaaagatgct tcgcctgcgg caggacagac cggaaccggt
    14401 ggagaatcac agacagactg ctgtgatgat gctgacgcca cggcttcggc tgacgggcag
    14461 accggcaagg gatcaggggc cggagaacag accattgaca aagaagcggc gttcctggac
    14521 gaaccggctt cgaagcgtac gatccacata ggctatgacg gcgggctttg ccaggcggca
    14581 atacctatag cgcaccacaa gggatttttt gaagctgaag gcctgaaaac agagcttacc
    14641 aggacaggcg gcacacatga caacgtgcgc gacgcgcttg cggccggaaa aatcgataca
    14701 actgcgggaa tgatcgcagg ctggcttaag cccatcacca acggcatcga cctcaagttt
    14761 acggtcggac tccacacagg gtgcgcttcg gcattcgtcc tggcagattc gccgatccgg
    14821 agctttgccg atgcgaaggg aaaaaccgtt gccatcaacg gcgggatcgg cggcgtatat
    14881 cacaacatag gttaccgttt tatcgcccat gacggatttg ctcccgaaga ctttatctgg
    14941 agagatttcc cggccgacca gacgctgctg atcctccaga agggagaagc cgatgttgcg
    15001 gtgttctccg accagctggc cgaaaaatgg gtgcaggacg gtactctgcg caggatccgt
    15061 tcgctgcacg aagacgaaga tttcaggaat gagtcctgct gcgtgcttgg catatccgga
    15121 tcattcatag ctgagaaccc ggtcgcgagc gagaaaatca gccgcgccat ttataaagcc
    15181 tccctgtgga tcggcgaaag cgatgcgaac aaagaagaag ccgtacagat tctgctggac
    15241 aacgattata taagcgggac gaaagagtat gcactgcagt tgatgaaact cttccggtgg
    15301 gggctgccca acgaactgac cgaggcaacg cttgaccttt ccataaaaga ttaccagagg
    15361 ctcggcgtga taagcgacag catcgaccct gccgaactca aggcacagat atgggtcccg
    15421 ctggatatcg aatgatcgtg caggcactga ctatataaac tgcaaaaatg aaatcgaagc
    15481 gaggtgtccc tcatgaaact taccgcctgg gaactgcaca ggctcgagag ttcgccgaag
    15541 tcaaaacttc ggcggacctt cgaaagaata cttacgatcc tgccgctgtt gctggtcatg
    15601 gcccacatgg cagagtacaa gctcatgcct gactttccga agtacgttgc gaacaggatc
    15661 gcatatcctg tcagcgacac atatacggtt tttcttggaa tactggcctt tgccctgctg
    15721 atatatctga tattgtcatt cttttcagac aggaaatatg agaggctggt gcactcatcg
    15781 ccgctatatt gtgtgattgc aataatcctt tcaatatatg atattgcaac gcttaaaacc
    15841 ggtattctgc agctgccgta ttttccgtgg gcagaccgga tactgcttgc cattatcgaa
    15901 gacaggaaga tgctgctcga ctgcgtcact aattcgctta tccttctgtt tgcaggctac
    15961 ctggcaggcg ccgccgccgg tctgttctgc ggcgtcggtg caggctggag caaaaagatc
    16021 aggtactgga tcatgccgct tctgaaactg cttggaccta tcccttccac tacgtggatc
    16081 tccatcatac tggttgttgc cagttccctg ttcgcgggca gcgttttcat gatcgcgctt
    16141 ggcgtatggt acccggtgac gatggcagca ctcaacggcg ttgccaatgt gaacaggagc
    16201 aacttcgagg tggcgaagac atttggcgca aacaggttcg aactggtctt caaagtggcg
    16261 atccctgccg ccatgccgag tattttcaac ggcctgacac agggaatggg cattgcgtgc
    16321 acggcgttga tggtcgccga gatgctcggg gtcgaatccg ggctgggatg gtatatcaac
    16381 tggcagcgcg gatgggccga gttcgcgaag atgtatgccg cggttataat catctgcctg
    16441 acgtttacgg ccgtaaattt tgtgctgacg ctgatcaaaa agcgtgtgct ccgctggaag
    16501 gaggagtatg tatgagcgga caggtggaaa tcaggaacct ttcaaaggta tttgcgcgac
    16561 ccggatcgga tgatacgctc caggcgctgg acaatatcag cctagacata cgcccgggag
    16621 aattcgtagc gcttcttggc gccagcggat gcgggaaaag cacgctgctg cgcatcattg
    16681 ccggccttga gatcccgacg aagggggagg tgcttttcaa cggcggcagg atatgcggca
    16741 cgagccccaa gcgcgggctc gtattccagg agcattcgct gttcgcctgg cttacggtac
    16801 ggcagaacat agagtttgcg ctgaaaactt caaggcagta ccaggacagg aaatgcgaga
    16861 tcgacagatg gctggaactt gccggactgg cggaatttgc ggggaactat ccgcaccagc
    16921 tgtcaggcgg gatgcggcag cgggccgcgc ttgtacgggc gctggcagtc tcgccggatg
    16981 tgctcctgct cgacgaaccg cttggcgcgc tcgacagttt cacccggatg agcctgcagg
    17041 acgaactggt ccgactctgg caggaacgcg gcaataccat ggtcatggtg acccatgata
    17101 tagacgaagc gatatatctt agccagcgca ttgtgataat gtcaccgcgg ccgggacgta
    17161 tcagcaggat catagaagtc cggcggagct atccgcgcga ccgggcttcg ggcgatttta
    17221 cagtgctgcg caatgaaata ctcaggttca tgaactttgc acatgaacag cagtatgatt
    17281 atgttatata aaaatatacc ggttcacgcc gggttgctgt gaaggcgaaa a
//
LOCUS       DAOSCC010000021        16998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2331_length_16998_cov_4.599894, whole genome shotgun sequence.
ACCESSION   DAOSCC010000021 DAOSCC010000000
VERSION     DAOSCC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_16998_cov_4.599894"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLG72_02270"
     CDS             <1..383
                     /locus_tag="PLG72_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ79661.1"
                     /translation="QKAARLKQLLVDGLASSDIEHRVNSPDDALPYIINISFRNVRAE
                     VLLHHLEQRGIFVSTGSACSSHKKNRSHVLTAMNVDPAMIDGALRFSLSHLNTEEDIR
                     LTLDALKDIIPVIDISKKKRSGRK"
     gene            383..1609
                     /gene="thiI"
                     /locus_tag="PLG72_02275"
     CDS             383..1609
                     /gene="thiI"
                     /locus_tag="PLG72_02275"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOJ79662.1"
                     /translation="MKKVILVRYGEIMLKGLNRPVFEDKLIRNIRRVLYGLGKTTVSC
                     SQARIFIEPEQDDFDFENAVSRLVKVFGIVSVSIVWKVASDFEEIKKYSAEMARDYVA
                     RLGRNTAAKNPAAESGTSGGAVGFKVESKRGNKRFPMESPEISRQLGGYLLKNVPGLK
                     VDVNNPDFIFFVEVREYTYIYCEIIPAFGGLPVGTSGKGVLLLSGGIDSPVAGWMMAK
                     RGMEIEAVHFHSYPYTSERAKDKVITLAKILSQYCQRIRLHIVPFTDIQLEINDRCPQ
                     EYLTIIMRRAMMKIAERIAAENGAIALITGESLGQVASQTAQALAVTNNAVSMPVFRP
                     LIGMDKNEVVEIARKIDTYETSILPYEDCCTVFVAKHPKTKPKLEEAKDYENRLDLDE
                     MIEKAVRYTETMVIGD"
     gene            1683..3074
                     /locus_tag="PLG72_02280"
     CDS             1683..3074
                     /locus_tag="PLG72_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ79663.1"
                     /translation="MTVARRKSLKWPIYAAILIIVAVSAAFLVLANKYTGDTIRKGII
                     IEGTDVSWLTAEDARTLVENGLKKNYADEKVTLAYGGRNWDIRLSDIDYRFEVDKTVE
                     QAFSIGREGNIFKRTFDAISLSRNGLALDVGVSFSREKLRSKLEEIKKQCDTAAKNAS
                     VTYNGGKFEYTPGVPSRVLDVDTNLKLVENYLTKRDFRNIELVVEEKEPEITYEEVKN
                     VNSVLSSFSTTFNSGDVNRTDNIKLACSRINNILLMPGDEFSMNRELGPRTYANGYKQ
                     APIIFKNELVPGTGGGVCQVSSTLYNAVLLTGLKVTEREHHSMPLTYISPGRDATITE
                     SSIDFRFMNNLKYPVLINANVKGNKLNISILGRKRDDGIEFRLKTKTVAVYKPKPEQI
                     VLDQTLEPGRKVVERKAKNGIRVILYREAYKNGVLQWREKLNEDYYRPVQGITRVSSD
                     IWHQYYAQGMPAD"
     gene            3194..3865
                     /locus_tag="PLG72_02285"
     CDS             3194..3865
                     /locus_tag="PLG72_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOJ79664.1"
                     /translation="MIEGLYSKEVVCPVCSQKFHATKVKARACKVKSRDSDFCVHYEG
                     INPILYDAWVCENCGYANQSEKFEEITQSEADILRKNLMPKWKKHSLSGERDVDTAIE
                     AFKLVLISHDFRGAKASEFAKVCLRIAWLYRYKGDSEEEAKFISHALDYYKQAYENEH
                     FPVDKLDESTCEYMIGELSFRAGNYSEAVKWFSRLIGSEEARKKPALIEAAREQYQLV
                     KEKLK"
     gene            3876..4856
                     /locus_tag="PLG72_02290"
     CDS             3876..4856
                     /locus_tag="PLG72_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOJ79665.1"
                     /translation="METGKLPCSVLEKLIFSKIKKVHDEILISPGIGLDCSAIDFGEY
                     ACVLSCDPITGTAKEIGRLAVHINCNDIASAGVLPLGLLAVILCPEDSTEEELDAIME
                     QFTEAARSVNVDILGGHTEITGAVNRFVISCTAVGKCLKDKIISASGAKNGDSLVITK
                     HAGLEGASIIAHEKEEELVPVLGRQTVEEAKSFMNSISVVKDGITAAKFGVNAMHDVT
                     EGGVLGAVWELCEASGTGAEVFLDKIPIHMATRKICEYYKIDPYRLISSGCMLISAAD
                     GEGLVSRLKQEGIDAAVIGKLDDSGRRLMLRDGRAEEIVPPASDELYKIL"
     regulatory      5044..5136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5229..6467
                     /gene="glyA"
                     /locus_tag="PLG72_02295"
     CDS             5229..6467
                     /gene="glyA"
                     /locus_tag="PLG72_02295"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ79666.1"
                     /translation="MYQIDEIRKIDPEVAQSIENEVERQRSKIELIASENFVDGAVLQ
                     ALGTPLTNKYAEGYPGKRYYGGCEHVDVIEQLAIDRAKAIFGAEHANVQPHSGAQANS
                     AVYFAVLEPGDLILGMDLAHGGHLSHGMKKNISGKYYRSEFYGVRKDTGTIDYDMLHK
                     KAMELKPKIIVAGASAYPRIIDFKAFSEIAKDCGAYLMVDMAHIAGLVAAGLHPNPVP
                     YADFVTTTTHKTLRGPRGGMILCKAEYAKMIDSAVFPGMQGGPLMHVIAAKAVSFKEA
                     MTEEFRKYQQQIVKNAIALADALMDEGFQLVSGGTDNHLMLVDLSNFSVTGKAAQLML
                     DEVGITVNKNAIPYDTQKPTVTSGIRIGTPAVTTRGMKEDDMREIARLIKMVITDFDN
                     SADLVRQKVAALCKKYPLYE"
     gene            6695..7984
                     /locus_tag="PLG72_02300"
     CDS             6695..7984
                     /locus_tag="PLG72_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOJ79667.1"
                     /translation="MDRKKEPLAYRMCPRTIDEFVGQEHIIGKGKLLYRMIKADRISS
                     IILYGPPGTGKTSLARVIAATTRANFEKLNAVTAGVADIKRIISDTQNPMFNQTGRTV
                     LFIDEIHRFNKAQQDALLPYVENGTIVLIGATTENPYFEVNKALISRSTVFMLKPLES
                     RDVERIIRMALTDSERGLGNYPLKVDEEAIRYLADISNGDARRALNALELAAVTTDLD
                     AEGQIHVTLEVIEECVQKKSIAFDKSGEAHYDNISAFIKSMRGSDPDAALFYLARALY
                     AGEDPEFLIRRIIICASEDVGMANPTALQVAVAAAQGIHMIGMPEARLLLAHAAVMVA
                     VSPKSNSCYKALEKAMHDVETKKTGEVPMHLRNAPVKGMEKLGYSIGYKYAHDYPGNY
                     VKQQYFPEGMEGTVYYEPTSNGYEARIKEWLERVRNN"
     gene            8099..8326
                     /locus_tag="PLG72_02305"
     CDS             8099..8326
                     /locus_tag="PLG72_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79668.1"
                     /translation="MVRRILAWVVLAGFILLLLNVMFFRFYWQLSVAIYIIVMLGFIL
                     YSSKARRMEDLKKVIKMEENNAGSNNEEDGE"
     gene            8399..9658
                     /gene="larA"
                     /locus_tag="PLG72_02310"
     CDS             8399..9658
                     /gene="larA"
                     /locus_tag="PLG72_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOJ79669.1"
                     /translation="MHFKYGYGKGYREFYIPDKNIMAELRQNNVDVGLTGLDEVMRAL
                     REPIASKRLSEIVKSGERAVIITSDITRPMPSKTVLPPVIDELLQAGARHEDITVVFA
                     LGSHRKHTEEEMRYLVGDDIYEKVRCVDSDVNRCRMLGTTSRGTPVEIFEEVADADRV
                     ICLGNIEYHYFAGYSGGAKAIMPGVSTRAAIQANHSAMVRDEARAGALDDNPVRLDID
                     EVVKFVPIDFIVNVVLDEEKNIIKAVAGHYLHAHREGCRFLDRLYKVAIPEKADIVIT
                     TPGGFPKDINLYQAQKALDNAKHAVRDGGIIILLASCSEGYGERVFERWINASDSPDS
                     LIESIRSNFELGGHKAAAIALVEKKARVFIVSDLDPCMCRRIYMEPFGSMQEALDCAF
                     SVMGQDARVLLMPHGGSTLPVVEQAGA"
     gene            9919..10350
                     /locus_tag="PLG72_02315"
     CDS             9919..10350
                     /locus_tag="PLG72_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ79670.1"
                     /translation="MKLSTKGRYGLKAMLDLAVHSADGQVSLNSIAERQGLSENYLEQ
                     LFASLKKARLVKSVRGPQGGYMLADSPENISVGDILRALEGSLAPADCVSEDLGATRC
                     GNSDKCVTRNVWERIRDGINNVIDNITLKELIDDYNRNNNN"
     gene            10428..11636
                     /gene="nifS"
                     /locus_tag="PLG72_02320"
     CDS             10428..11636
                     /gene="nifS"
                     /locus_tag="PLG72_02320"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOJ79671.1"
                     /translation="MSTRTIYLDHAATTPVKKEVLEAMLPWFTENYGNPSTVYSAGRT
                     SKKAIEEARETVARIIGAEPREIFFTGSGTEADNWAIKGAAYANMKKGKHIITTSIEH
                     HAVLHTCQYLESEGFEVTYLPVDEYGLVTPEQVEAAVRPDTILISIMFANNEIGTIQP
                     IAEIGKIARAKGICFHTDAVQAMGNIRINVSDLNVDMLSMSAHKFYGPKGVGALYIRK
                     GVKLTSFMHGGAQERGRRASTENVPGIVGLAKALELADRNMDEYNRKLIALRDRTIEE
                     IEKKIPFARLNGHREKRLPGNVNFSFEFIEGESLLLMLDLKGIAASSGSACTSGSLDP
                     SHVLLAIGLPHEKAHGSLRLTFGEENTDEDVDYLMEVLPGIVQRLREMSPLYEEIRNK
                     NSDGQECNKN"
     gene            11706..12158
                     /gene="nifU"
                     /locus_tag="PLG72_02325"
     CDS             11706..12158
                     /gene="nifU"
                     /locus_tag="PLG72_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOJ79672.1"
                     /translation="MMYSEKVMDHFMNPRNVGEIADADGVGQVGNPKCGDIMKMYLKI
                     ENDVITDVKFKTFGCGAAVATSSMATELVKGKTIDEALKITNKAVAEALDGLPPVKMH
                     CSVLAEEAIKAAIDDYKRRKGLIDDSGACDVCCKRCEDIHHHLNDEDE"
     gene            12166..13254
                     /gene="mnmA"
                     /locus_tag="PLG72_02330"
     CDS             12166..13254
                     /gene="mnmA"
                     /locus_tag="PLG72_02330"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOJ79673.1"
                     /translation="MSKGKVMLGMSGGVDSSVAAAILKEQGYEVIGVTMQIWPDMDED
                     RRQTEGGCCSLSAVDDARRVANRLGINYYVLNFKDVFQQKVIDYFTNEYLAGRTPNPC
                     IACNRFVKFEALLAKAVSMGIDYVATGHYARIAYDDASGRYLLKKSVTAEKDQTYVLY
                     TMTQYQLERTLFPIGEYKKDQVRALAREYGLPVASKPDSQEICFVEDNDYGRFITENT
                     DAEINPGYFIDRDGNILGRHRGIIHYTIGQRKGLGVSFGKPMYVVDIRPEDNTVVLGG
                     ADDVFSGGLIASDPNFISMDKLNDEMRVTAKIRYSAKEAPAIIRPLDVNRVEVTFDEP
                     QRAITPGQAVVFYDGDTVVGGAVIDRSF"
     gene            complement(13370..14164)
                     /locus_tag="PLG72_02335"
     CDS             complement(13370..14164)
                     /locus_tag="PLG72_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOJ79674.1"
                     /translation="MFDCHTHTTFSTDSVMSAELACESAIELGLDGIAFTDHLDYDFP
                     GEMDFLIDFDEYFSILDPVREKYEDRLKVLVSVEVGIQPHVLRESEEVIRRYPFDYVL
                     ASVHLVDRQNPYDKEYYRGKSKKEAYSRYLEEIYFMVKNLESFDMLGHFGYITRYADY
                     ADRTLRYADHSDLFDMIFKELIDHGRGFELNTGTYRIERPDAVYDTEILKRYRQLGGE
                     LVCLGSDAHQADHIAARFGYFAQVLRDAGFKYTVHFEDRKPVFDKL"
     gene            14347..15780
                     /locus_tag="PLG72_02340"
     CDS             14347..15780
                     /locus_tag="PLG72_02340"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ79675.1"
                     /translation="MSDAKTEGQKLEERLSYKRRNAWEKVDEEYIKKAFDFCEKYKDF
                     LNASKTEREFAANTEKLAVAAGFKKLDDLIASNKRLKPGDKVYHINRKKSAVLAVIGK
                     KPLSEGVNILGAHIDSPRLDLKQNPLYEDTEMAFFKTHYYGGIKKYQWLTIPLAIHGV
                     VVKGDGTSVEIVIGEDDDDPVFSITDLLPHLAREQMQKKMGEAVSGEGLNVLLGSMPF
                     NDEKVKEKVKLNILDILNKRYGIVEEDFQSAELELVPAQKFRDVGFDKSLVGGYGQDD
                     RVCAYPALKAILDIDTPERTAVCLLTDKEEIGSMGNTGAQSNLLQDFLANIIALADDA
                     YSDLKLRACLSKSRMLSADVNAAVDPNYESVYEKRNATYLGHGIVLQKYTGARGKYDS
                     SDASAEFVGEVRKLFNDNGIFWQTGELGKVDEGGGGTIAQYAANLGIDVLDCGLSVLS
                     MHSPFEITSKIDIYITYNAYKVFCSIK"
     gene            complement(15899..>16998)
                     /locus_tag="PLG72_02345"
     CDS             complement(15899..>16998)
                     /locus_tag="PLG72_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ79676.1"
                     /translation="ILTIVAAVVLFVFGSGILYYINTNIEIISSDVFASGDQDNHLAH
                     IINPLVTKKEPFNILLLGGDKVNNLTDTMMLANFDPETCKINIMSIPRDTRVFINNKE
                     RKINYAFPNGGVELSAKTVSELLDVNIKYYAFVDTSAFRKIIDLLDGVDYYVPVDMDY
                     DDPVQNLHIHLKKGQQRLYGEQAEQYVRFRKPNKWNKEIRKYYDGSDLKRNEAQQQFI
                     RELMRQKLTLQYLPKLTSVINTMFEHVQTNLTLNETVKLTSYITKFKIENVNFIQMPG
                     TTYDASPYYYICDVQAARQIMAENFKCDESFISADPDVKAFYLTKKAKYQPPQPKPAE
                     NEQQASNTGNGEVSGNPSNSETLITGSDQDP"
BASE COUNT         4947 a         3678 c         4509 g         3864 t
ORIGIN      
        1 atcaaaaggc agcacggctc aaacagttac tggtggacgg acttgcgtcg tctgatatcg
       61 aacaccgcgt caactcgcct gacgatgctt taccctatat aataaacatt tcgtttagga
      121 atgtcagggc ggaggtcctg ctccatcacc tggagcaaag gggcattttc gtttcgaccg
      181 ggtcggcctg ttcctcacat aaaaagaaca ggagccatgt cctgacagct atgaacgtcg
      241 atcctgccat gatagacggc gctttgagat tcagtctatc gcatctgaat accgaggaag
      301 atatcaggtt gacattagat gcattgaagg atatcattcc ggtaatagat atcagcaaaa
      361 aaaagagatc cgggaggaag taatgaagaa agtcatactt gtcagatatg gcgagataat
      421 gctcaagggt ctgaacagac cagtatttga agacaaactc atacgaaaca tcagaagggt
      481 tttgtacgga cttgggaaaa caactgtaag ctgttcgcag gcgaggatat ttatcgaacc
      541 ggaacaggat gattttgatt tcgagaacgc cgtatcaagg cttgtaaaag tattcgggat
      601 cgtttctgtc agtatcgtat ggaaggttgc atctgacttc gaagaaataa agaagtattc
      661 tgccgagatg gccagggact atgtcgcaag gctgggcagg aataccgcag caaagaatcc
      721 ggcagcggaa tccggaactt ccggcggcgc cgtcggattc aaggtagaat ccaaaagggg
      781 caacaagagg tttccaatgg aatcccccga aatatccagg cagcttggcg gatacctgct
      841 gaaaaacgta cccgggctga aagtcgatgt aaacaacccc gacttcattt tctttgtcga
      901 agtcagggag tacacatata tttattgtga aataatcccc gcattcggag gactgccggt
      961 aggcacaagc ggtaagggcg tgctgctgct ttcaggaggt atcgacagtc cggtggccgg
     1021 atggatgatg gcaaaaaggg gcatggagat agaagcggta catttccaca gttatcccta
     1081 taccagcgaa cgtgcaaagg acaaggtcat tacgctggca aagatccttt cgcagtattg
     1141 ccagcgtatc aggctccata tagttccgtt cactgatata cagcttgaaa taaatgacag
     1201 gtgccctcag gaatacctta cgataatcat gcgcagagcg atgatgaaga tagcggaaag
     1261 gatcgccgct gagaacggcg ctatagctct catcaccggt gaaagcctcg ggcaggtcgc
     1321 cagccagacg gcgcaagccc ttgcagttac gaacaacgct gtaagcatgc cggtattcag
     1381 gcccctgatc gggatggaca agaatgaagt ggtggaaata gcaagaaaga tcgatacgta
     1441 cgagacatcg atccttccat atgaagactg ctgcacggtt ttcgtggcga aacaccccaa
     1501 aacgaaaccg aagctggaag aggcgaagga ttatgaaaac aggctcgatc ttgacgaaat
     1561 gatagagaaa gctgtcaggt atacggaaac tatggtcatt ggtgactaat tccacgcatg
     1621 tgcggcagac cgttatgaca acaatatact tgtgcagagt gttacaggaa aggttgttgc
     1681 caatgacggt cgctcgcagg aaaagcctga aatggccgat atacgccgcg attttaataa
     1741 ttgtcgccgt ttctgctgcc tttttggttc ttgcaaataa atatacagga gatacgatcc
     1801 gaaaaggcat catcattgag ggtacagacg tttcatggct tactgcagag gatgccagga
     1861 ccctggtgga aaacgggctt aaaaaaaatt acgccgacga aaaagtgacc ctggcatatg
     1921 gcgggcgaaa ttgggatatc aggctgagcg acatcgatta cagattcgag gtcgacaaaa
     1981 cagtcgaaca ggcattcagc atcggacggg aaggcaacat attcaaaagg acatttgatg
     2041 cgatctcgct ttcaagaaac gggctggcgc tggatgtagg agtgagcttc agcagggaga
     2101 agctcaggag caaactggaa gagatcaaga aacagtgtga tactgctgcc aaaaacgcat
     2161 cagtcaccta caacggaggg aaattcgaat atactccagg tgtcccgtcc agggtgcttg
     2221 atgttgacac caatctcaaa ttggtggaaa attatttaac aaagagggat ttcaggaata
     2281 ttgagcttgt tgtggaagaa aaagagcctg aaataacata cgaagaagta aagaatgtca
     2341 acagtgtgtt gtcgagtttc agcaccacgt tcaacagcgg cgatgtcaac aggaccgaca
     2401 acataaaact ggcttgcagc aggataaaca acatactgct gatgccgggg gatgagtttt
     2461 cgatgaacag ggaactgggg ccgaggactt acgcaaatgg ttacaaacag gctcccatca
     2521 tttttaagaa cgaactggtc cccggcaccg ggggaggtgt atgccaggtt tcgtcaacgc
     2581 tctataatgc ggttttgctg acaggactta aagtaacgga acgggaacac cattcgatgc
     2641 cgctgacata tatcagcccg ggaagagatg caacgatcac ggaaagctca atagacttca
     2701 ggttcatgaa taatctgaag tatccggtgc ttataaatgc gaatgtgaaa ggcaataaac
     2761 tgaatataag catacttgga aggaaaaggg acgacgggat agagttcagg ctgaaaacga
     2821 aaacggttgc cgtttacaag ccaaagcctg agcaaatagt acttgaccag acacttgaac
     2881 ctggccggaa agtcgttgaa cgcaaggcaa agaacggcat cagggtgata ttatacaggg
     2941 aagcctataa gaacggcgtg cttcaatggc gtgaaaaact gaatgaagat tattacaggc
     3001 ctgttcaggg catcacaaga gtaagcagcg atatctggca ccagtattat gcgcagggaa
     3061 tgccggccga ctgaccaccg caggcattac acaggatgac acagagatct gtgcgacgga
     3121 tcggcccggg gttttcctgc cataacagac ggagccggtt gatataaaag agagaatgaa
     3181 atgaggggtc atgatgatcg aaggactgta cagcaaggag gtcgtatgtc ctgtctgttc
     3241 gcagaaattc catgcgacaa aggtaaaagc gagggcatgc aaggtcaaaa gcagggattc
     3301 cgatttttgt gtacattatg aaggcatcaa tcccatttta tatgatgcct gggtctgcga
     3361 aaactgcggt tacgcgaacc agtcggaaaa atttgaagaa ataacgcaga gtgaagccga
     3421 tatcctcagg aaaaatctta tgccgaaatg gaagaagcac agcctgagcg gagaacggga
     3481 tgtggatacg gcgatagaag cattcaaact tgtactcata agccacgatt tcagaggagc
     3541 aaaggccagc gaattcgcaa aggtatgtct caggatcgcc tggctgtaca gatacaaggg
     3601 cgacagtgaa gaagaagcca aattcatcag ccatgccctg gattactata aacaggcgta
     3661 tgagaatgaa catttcccgg tcgacaagct cgatgaaagc acctgcgaat atatgatcgg
     3721 cgaactgagt ttcagggccg gcaattacag tgaagctgta aaatggttca gccggctcat
     3781 aggttcggag gaagcaagaa aaaagcctgc gctcattgaa gccgcaaggg agcagtatca
     3841 actggtcaag gaaaagctga aatgacggag gtagtatgga aacaggaaaa ttgccatgca
     3901 gtgtgcttga aaagctgata ttcagcaaaa tcaaaaaagt gcatgatgaa atactgatca
     3961 gtccgggtat tggcctggac tgctctgcga tagatttcgg cgaatatgca tgcgtccttt
     4021 catgcgatcc catcaccggg accgcaaaag agatcgggcg gcttgcagta cacatcaact
     4081 gcaatgatat agcttccgcc ggcgtgctgc ccctcgggct gcttgccgtc attctttgcc
     4141 ctgaagattc aacggaggaa gaactggatg ccataatgga gcagttcact gaagcagcaa
     4201 ggtccgtaaa tgtagacata cttggcgggc atacggaaat caccggcgcc gtcaacagat
     4261 tcgtcatatc atgcacggca gtgggcaaat gcctgaaaga taaaatcatc agcgccagcg
     4321 gcgctaaaaa cggtgacagt ctcgtcatca caaagcatgc cggcctggag ggcgcttcga
     4381 tcatagccca tgagaaggaa gaagaactgg taccggtact tggcagacag acggtcgagg
     4441 aagccaagtc cttcatgaac agcataagtg ttgtgaaaga cgggataacg gctgcgaagt
     4501 tcggcgtgaa tgccatgcat gatgtgaccg aaggcggagt gctcggagct gtctgggaac
     4561 tctgcgaagc atccggtacc ggcgccgagg tattcctgga taaaataccg atccatatgg
     4621 caacccgaaa aatatgcgaa tattataaaa tagatccata caggcttata tccagcggct
     4681 gcatgcttat atcagcagca gacggggaag ggctcgtcag caggctgaag caggagggta
     4741 tcgacgctgc agttatcgga aagctcgacg attccggcag gaggctgatg ttaagggatg
     4801 gcagggctga agagatagtc ccgcctgcaa gtgatgagtt atataaaatt ttgtaacgag
     4861 gctttagtat gcctgaagcc tcgttgaaac accgctagag tgaggaattt ttctgcaacc
     4921 gaaaattcct gtgaatattg tgttataact tgacaggatt ttgtcaaatg ctataatatt
     4981 tttattaaat tttataaaaa cgcggatgat ttccatggaa ggacaggttg tccgactgtg
     5041 gagggacgca cctctggaga gaccgtcaag gcgccgaagg ggcaagtccc gtggacacgg
     5101 gatacaactc tcaggcaaaa ggacagagga tgctcatatc atagttctat gatatttggg
     5161 tatcacaggt caaatccgag ggtttgacct gtatttttta ataaaaaaac gtaaaatgga
     5221 ggtatatcat gtatcagatt gatgaaatac gaaagatcga ccctgaagtt gcgcaaagta
     5281 ttgagaatga agtcgaaagg cagaggagca aaatagagct catcgcttcg gaaaatttcg
     5341 tggacggagc tgtattgcag gctcttggga ctccccttac caataaatac gccgaaggat
     5401 acccgggaaa aaggtattac ggaggctgcg agcatgtcga cgttatcgaa cagctggcaa
     5461 tcgacagggc caaggccatt ttcggcgcag agcatgccaa tgtgcagccg cattccggcg
     5521 cacaggcaaa ttcggcagtg tactttgcag tgctggagcc cggtgatctt atactcggca
     5581 tggatcttgc ccacggcgga cacctgtccc acggcatgaa gaagaacata tcgggaaagt
     5641 actacaggtc tgagttttac ggcgtaagaa aagatacggg caccatcgac tatgacatgc
     5701 tccataagaa ggccatggaa ctcaagccga agatcatagt cgcaggagcg agtgcttacc
     5761 cgcgcataat cgattttaag gcattcagcg agatagcaaa ggactgcggt gcttacctta
     5821 tggtagacat ggcgcatatc gcaggactcg tcgcagcagg tctccatccg aatccggttc
     5881 cgtacgcgga tttcgtaact acaacgaccc acaagacatt gagaggcccg cgcggaggaa
     5941 tgatactctg taaggctgaa tatgcgaaaa tgatcgattc agccgtattc ccaggcatgc
     6001 agggcggtcc gctgatgcat gtgatagctg caaaggcagt tagtttcaag gaggctatga
     6061 cagaagaatt cagaaaatac cagcaacaga tcgtcaagaa tgcaatagcg ctggccgacg
     6121 cactgatgga tgaaggattc cagcttgttt ccggaggtac ggacaatcat ctgatgcttg
     6181 tcgatctgag caacttctcc gttacaggca aggctgccca gcttatgctc gatgaggtcg
     6241 gcatcacggt caacaagaat gcgatacctt atgatactca aaaaccgact gtaacaagcg
     6301 gtataaggat aggcactcct gccgtcacca ctaggggcat gaaggaagat gacatgagag
     6361 aaatagcaag gcttattaag atggtcatca cagatttcga caactcggca gacctggtaa
     6421 ggcagaaagt agccgctttg tgtaaaaagt acccgcttta tgaatgatgg acggtaaaat
     6481 atttgtggtc agtcaagttg tgcggatgga ttggttaata ttgaaaatcc ggtcagcaag
     6541 ttgctattga ccaatgtaca tgttaatgtt gaattaagtt caccgaaggg ctaagatagt
     6601 aggagaacga cctttatctt ggtccttttc ttaaattgcg cacggcttga atgatagttg
     6661 ccagtattat agccttgttc tgcgggaggg tgacatggat cggaaaaaag aaccgctggc
     6721 atacaggatg tgtcccagga ccatcgatga attcgtcggg caggagcaca tcatcggcaa
     6781 aggaaaactg ctttaccgga tgataaaggc agacaggata tcatcgataa tactctatgg
     6841 tcctccaggc acaggcaaga cttcccttgc aagggtcata gcagccacga ccagggccaa
     6901 ctttgaaaag ctcaatgccg ttaccgcagg agtggcggat ataaaaagga tcatatccga
     6961 cacccagaat cccatgttca atcagaccgg caggacggtg cttttcatag atgagataca
     7021 caggttcaat aaagcccagc aggatgcgct gctgccatac gtggaaaacg gcaccatcgt
     7081 acttataggc gcaacgaccg aaaatcctta tttcgaagtg aataaggcgc ttatctcacg
     7141 ttcgaccgtc tttatgctaa aacctctcga aagcagggat gtggagagga tcataaggat
     7201 ggccctgacc gacagcgaaa gagggctcgg caactatccg ctaaaggttg atgaagaagc
     7261 gatcaggtac cttgcagata tctcgaacgg cgatgccaga cgggcgctca atgcgcttga
     7321 actggctgcc gtcaccaccg atctggatgc ggaaggacag atacatgtaa cccttgaagt
     7381 aattgaggaa tgtgtccaga aaaaatcgat agcttttgat aagtccggtg aagcgcatta
     7441 tgacaacatc agtgcgttta taaaatcgat gaggggcagc gatccggacg cggcgttgtt
     7501 ttaccttgca agagccctgt atgcgggaga agatccggag ttcctcataa ggaggatcat
     7561 tatatgcgca tcggaagatg tcggcatggc gaacccgacg gcgctgcaag ttgcagttgc
     7621 tgccgcacag ggcatacaca tgatcggaat gccggaggcc aggcttttgc tggcccatgc
     7681 agcggtaatg gtggcagtca gcccaaaatc gaactcatgc tataaagcgc tggaaaaagc
     7741 aatgcatgat gtggaaacga agaaaacagg agaagtgcct atgcacctca ggaatgcgcc
     7801 tgtgaagggc atggagaagt taggttacag tatcggttat aaatatgccc atgattatcc
     7861 tggcaattat gtaaaacagc agtatttccc ggaaggcatg gaaggaacgg tctattacga
     7921 acctacttcc aatggatatg aagccaggat aaaggaatgg ctcgaaaggg tcaggaacaa
     7981 ttaaaaggca gcacagtaag tttgaatacc aggtttattt cactggaata taacgctgcc
     8041 ttgaataata aatactgttg cggaacttgg acttaagcat ggagaaacgt agggaaatat
     8101 ggtcagaagg atattggcct gggtggtcct ggcgggattc atactgcttt tgctgaatgt
     8161 tatgttcttc agattctatt ggcagctcag cgtggcaata tacatcatag tcatgctggg
     8221 gttcatcctg tacagcagta aggcaaggcg gatggaagat ctgaaaaagg tcataaaaat
     8281 ggaagaaaat aacgccggaa gcaataacga agaggatggg gagtgatacc taagaagacc
     8341 ggctgccggg aaggcattcc aatgcattaa aacttgcagt tgacagggag gcaaacatat
     8401 gcatttcaaa tatggatatg gcaaaggata cagagaattt tacatacctg acaaaaacat
     8461 aatggcggaa ctgcgccaaa acaatgtaga tgtcggactt accggtcttg acgaagttat
     8521 gcgtgcactg agggagccta tcgcgtcgaa gaggctatca gaaatcgtaa aaagcggcga
     8581 aagggcagtt ataatcacca gcgacatcac aaggccgatg ccgagcaaaa cagtacttcc
     8641 gcctgtcatt gatgaactcc tgcaggccgg cgcaagacat gaggacataa ctgttgtatt
     8701 tgccctgggc agccacagga aacataccga ggaagagatg aggtaccttg tcggcgatga
     8761 tatttatgaa aaggtgagat gcgtcgacag cgatgtgaac aggtgcagga tgctcggcac
     8821 gacttcaagg ggaacgcctg tggagatatt tgaagaagtc gctgatgcgg accgcgtcat
     8881 atgccttggg aatatcgaat accactattt cgccggatac agcggcggcg ccaaggcgat
     8941 aatgccaggc gtttcaacgc gtgctgccat ccaggcgaac cacagcgcaa tggtgcggga
     9001 tgaggcaagg gcaggcgcgc tggacgacaa tccggtcaga cttgatatcg atgaagtggt
     9061 taagttcgta cccatcgact ttatcgtgaa tgtcgtgctg gatgaggaaa aaaacatcat
     9121 aaaagccgta gcaggccatt acctgcatgc ccacagggag ggctgccgtt tccttgacag
     9181 gctgtataag gttgccatac ctgaaaaagc ggatatagta atcacaacgc ccggaggatt
     9241 cccgaaggat ataaaccttt accaggccca aaaagcgctg gacaacgcga aacacgccgt
     9301 ccgcgacggc gggatcataa tccttctggc atcgtgcagc gaagggtacg gcgagagagt
     9361 tttcgaaaga tggatcaacg catcggattc accggacagc ctcatagaaa gtatcaggag
     9421 caatttcgag cttggcggtc acaaggccgc agccatagcc cttgtcgaaa agaaagcccg
     9481 ggttttcatc gtttcagatt tggacccatg catgtgcagg cgtatttaca tggaaccgtt
     9541 cggcagcatg caggaagccc ttgactgcgc attttccgtt atggggcaag atgcccgcgt
     9601 gcttctcatg ccgcacggcg gatccacgct gccggtggtt gaacaggcag gtgcctgata
     9661 cttcattcca ggacaagggg acggttctcc tgtctgacgt acgacacact tcggacagga
     9721 aaaccgtttt tattaagtgc ttttgctttt gcttttccga ttttcttctg tcccatgcca
     9781 ggctattctt gattacatgc cggatgttat ttttctattg acaaattaaa aactaaatgt
     9841 taatctttac ttgaataacc gagtaattta ctaggaatta tcgaaggatg cacggcaggc
     9901 atcatcggag gtaaaacgat gaagctttca acaaaaggcc gttatggctt aaaagcgatg
     9961 ctggacctgg cagtccacag cgccgacgga caggtatccc tgaacagcat agcggagcgc
    10021 cagggattat cggagaatta tcttgagcaa ctgttcgcct cattgaagaa ggcaaggctt
    10081 gtaaaaagcg tgagaggacc gcagggcggc tacatgcttg cagacagccc ggaaaacatc
    10141 agcgtgggcg acatactgag agccctggag ggatcccttg ctcctgcaga ctgtgtatcg
    10201 gaagaccttg gcgcaacccg gtgcggcaac tctgacaaat gtgtcacgag gaatgtttgg
    10261 gaaaggatcc gggacggtat aaataatgtt atcgacaaca taacactcaa agaattgatc
    10321 gatgactata acaggaataa taacaattag attattgaca atacttgaaa tatatcaggt
    10381 tttcaaaaac tggcgatata catttttcat gaaaaggaga cagaagtatg agcacaagga
    10441 ctatatatct cgaccacgca gcaacgacac ctgtaaagaa ggaagtactg gaggcgatgc
    10501 ttccctggtt cactgagaat tacggcaatc cgtccacggt atattcagca ggcaggacga
    10561 gcaaaaaggc cattgaagaa gccagggaaa cggtagcgcg tatcatcggg gcggaacccc
    10621 gtgagatatt cttcacaggt tcgggcacag aggcggacaa ctgggctatt aaaggcgctg
    10681 cctatgcgaa catgaagaag ggcaagcata taataaccac ttccatcgag catcacgcgg
    10741 tgctccatac atgccagtat ctcgaaagtg aaggcttcga ggtgacatac cttccggtgg
    10801 atgagtacgg acttgtcact cctgaacagg tggaagcagc cgtcaggccg gatacgatcc
    10861 ttatttcaat tatgtttgcc aataacgaga tcgggaccat acaaccgatc gcagagatag
    10921 ggaaaatcgc aagggcgaag gggatatgct tccataccga tgccgttcag gcaatgggga
    10981 atatacggat aaatgtttcg gacctgaatg tggacatgct ttccatgtca gcccacaagt
    11041 tctatggccc taagggagtc ggtgctcttt acataaggaa gggcgtcaag ctgacttcct
    11101 tcatgcacgg cggcgcccag gaaaggggca gacgcgcaag caccgagaac gttccgggca
    11161 tcgtgggcct ggcaaaggca ctggaactgg cagacaggaa catggatgaa tacaacagga
    11221 agcttattgc cctgcgtgac aggaccatcg aggaaatcga aaaaaagata ccttttgcga
    11281 gactgaacgg ccacagggaa aaaaggcttc cgggcaatgt caatttctca tttgaattca
    11341 tcgaaggcga gtcccttctg ttgatgctgg atctgaaagg catagcggct tccagtggtt
    11401 cagcctgcac gtcaggttca ctggatccgt cgcatgtgct cctcgccatc gggcttcctc
    11461 atgagaaggc gcatggatcg ctgaggctca ctttcggaga ggaaaatacg gatgaggatg
    11521 tggactatct tatggaggta ctccccggga tagtccagag actgagggaa atgtcgccac
    11581 tttatgaaga gatacgcaat aaaaattctg acgggcagga atgcaataaa aactgatatc
    11641 gttataatac acagttttta agcttgattg ctgggtgtta tacagacaga aagagggatt
    11701 gatttatgat gtatagcgaa aaagtaatgg accatttcat gaacccgaga aatgtcggag
    11761 aaatagccga tgcggacgga gtaggacagg tcggcaaccc gaagtgcgga gatataatga
    11821 aaatgtatct gaaaatagag aacgatgtca ttaccgacgt caagtttaaa accttcggtt
    11881 gcggagccgc tgtcgctacg agcagcatgg caacggaact cgtgaagggc aagaccatcg
    11941 atgaggcgct gaagataacc aataaggcag tggcggaagc acttgacggt ctgccgccgg
    12001 tgaagatgca ttgttctgtg ctcgcagagg aagcgatcaa agccgcgatc gacgattata
    12061 aaaggagaaa aggcctgata gatgacagcg gcgcatgtga cgtatgctgc aagagatgtg
    12121 aggatataca ccaccacttg aatgatgaag acgaataggt gaaacatgtc aaagggtaaa
    12181 gtaatgctgg gcatgagcgg cggagtcgac agttccgtcg ctgctgcgat tttgaaggaa
    12241 caaggttacg aagtcatcgg agtaacgatg cagatatggc cggatatgga cgaagaccgg
    12301 aggcagacag aaggcggatg ctgttctctt tccgcagtgg atgacgcccg cagggtcgcg
    12361 aacaggctcg ggataaacta ttatgtattg aacttcaagg atgtattcca gcagaaggtc
    12421 atagactatt tcactaacga ataccttgcg ggcaggacgc cgaacccctg catcgcctgc
    12481 aaccgtttcg tcaagttcga agcactgctg gcaaaagctg tttcaatggg gatcgattat
    12541 gtagccacag gccattacgc ccgtatcgca tatgatgatg catccggcag gtacctgctg
    12601 aagaagtccg ttaccgctga gaaggaccag acatatgtcc tgtacacgat gacccagtac
    12661 cagctggaga ggacgctatt cccgataggc gaatacaaga aagaccaggt gcgtgccctt
    12721 gcaagggaat acggccttcc ggtagcatca aagccggaca gccaggagat atgctttgtg
    12781 gaagataacg attacggcag gtttatcacc gagaataccg atgcggagat aaatccaggc
    12841 tattttatcg acagggacgg caacatcctt ggcaggcaca gggggatcat ccattacacc
    12901 ataggccaga ggaaaggcct tggagtatcc ttcgggaagc cgatgtatgt cgtggatata
    12961 aggcctgagg acaacactgt ggtgcttggc ggcgcagatg atgtcttttc cggcggactg
    13021 atagcttcgg atccaaactt tatttccatg gacaagttga atgatgagat gagagtaacc
    13081 gcaaagatca ggtattccgc caaagaggct ccggcaatta tcaggccgct ggatgtgaac
    13141 agggtggagg taacgttcga tgaaccgcaa agagccataa ctcccggcca ggctgttgta
    13201 ttctatgacg gagacacagt ggttggaggc gctgtgatag acaggtcgtt ctgaacgaat
    13261 aatgtggcaa ggatgcgggg aaggtcctcg tttcaaactc aatatcggga tttatacaca
    13321 aggaccgtcc ccacttcccg ggcaatgatt gtcgtgcaag tcaacgtact tacagcttgt
    13381 caaaaacggg tttcctgtct tcaaaatgca ctgtgtattt aaaccccgca tcacgcaaga
    13441 cctgggcaaa atatccgaac ctggccgcta tgtggtctgc ctggtgcgcg tcggaaccga
    13501 ggcatacaag ttctccgccc aattggcggt accttttgag tatttcagta tcataaacgg
    13561 catcgggcct ttcgatcctg tatgtgccgg tattcagttc gaatcccctg ccatggtcta
    13621 tgagttcctt aaatatcata tcgaaaagat ccgagtggtc agcatatctt aacgtcctgt
    13681 cggcataatc ggcatatctt gttatgtatc cgaaatggcc cagcatatcg aaagactcaa
    13741 ggtttttaac catgaaatat atctcttcaa gataccgcga ataagcttct ttctttgatt
    13801 ttcccctgta atactctttg tcgtacggat tctgtctgtc tacgagatgt acggatgcaa
    13861 gcacgtaatc gaacgggtac ctgcggatca cttcttcaga ctcgcggagc acatggggct
    13921 gtatcccaac ctccacggaa acgagcactt tcagacggtc ctcgtatttt tccctgacag
    13981 ggtccagaat actgaaatac tcgtcaaagt ctatcaggaa atccatctct ccgggaaagt
    14041 cataatccag atggtctgtg aaagcgatac cgtcaaggcc aagctctatt gctgattcgc
    14101 aagccagctc agcactcatc acactgtctg tcgagaatgt agtatgtgta tggcagtcaa
    14161 acataatttc cctccatggt ctttttccgg agacaatact atcactgtaa atggataaaa
    14221 tcaatgccaa aaacagcggt catgtcaatt caagaaatat atggtttata aaatggaaac
    14281 gtaggtatat aataaaggaa gtccgatatt ttcgactaaa cataccaata caggaggaat
    14341 ctgatcatga gcgatgcaaa gaccgaagga cagaaacttg aagaacggct ttcctacaag
    14401 aggcggaatg cctgggagaa agtcgatgaa gaatatatca aaaaggcatt tgatttttgt
    14461 gagaaatata aagatttcct caacgcgtcg aaaaccgaac gcgaatttgc cgcaaatact
    14521 gaaaaacttg cagttgcggc cggattcaaa aaactggacg atctgatagc ctcaaacaag
    14581 aggttaaagc caggggacaa ggtttatcac ataaaccgta agaaatccgc cgtcctggcc
    14641 gttataggca aaaagccgtt atcggaaggt gtcaatatcc tcggcgccca tatcgattcg
    14701 ccgcgcctgg atctcaagca gaatccgctt tatgaagata ctgaaatggc ctttttcaag
    14761 acacattatt acggcggtat caagaagtac cagtggctca ccatacctct cgctatccat
    14821 ggcgtcgtgg taaagggtga cggtacgtca gtcgaaattg tgatcggtga agatgacgac
    14881 gaccctgttt tcagtataac cgacctgctg ccccaccttg cccgcgagca gatgcagaag
    14941 aaaatgggcg aggcagtgtc aggggagggg ctcaacgttc ttctcggttc gatgcccttc
    15001 aatgatgaaa aggtaaaaga gaaggtgaaa ctgaatatac tggatatcct taacaagagg
    15061 tacggtattg tcgaggaaga cttccagtcg gcagagctcg aactggtgcc tgcccagaag
    15121 ttcagggacg tcgggttcga taaaagcctg gtcggaggat acggccagga tgacagggtc
    15181 tgcgcgtatc ctgccctgaa ggccattttg gatatcgata ctcccgaacg tacggcagta
    15241 tgtctgctga cagacaagga agagatcggc agcatgggca ataccggtgc acaatccaac
    15301 ctgctccagg attttcttgc aaatatcatt gcactggcag acgatgccta ttctgatctt
    15361 aaactccgtg catgcctttc gaagtccagg atgctgtctg ctgatgtgaa cgcagctgtt
    15421 gacccaaact acgaaagtgt ttacgaaaaa cgtaatgcaa cgtatcttgg ccatgggatc
    15481 gtactacaga agtacaccgg ggccaggggg aaatacgatt ccagtgatgc aagcgccgag
    15541 tttgtcggag aagtacggaa acttttcaat gacaacggca tcttctggca gacaggcgaa
    15601 ctggggaaag tggacgaagg gggaggaggc acgatagcgc agtatgcggc aaatctcggc
    15661 atagatgtcc ttgactgcgg cctttcagtg ctttcgatgc attctccgtt cgagatcacc
    15721 agcaagatcg acatctatat aacatacaat gcctataagg tattctgcag cattaagtaa
    15781 tcgtatacga tagcaggaat aaagtccggc agtctgccgg actttatttt ttgatccagg
    15841 ataggtcgtt ataaaatttt aaagtgatcc gaattatcag aaatatgatc aaagcacatc
    15901 acggatcctg atccgagccg gttattaaag tttcgctgtt cgatggattc ccggaaacct
    15961 ctccgttgcc ggtattactt gcctgttgct cgttttctgc cggtttgggc tgaggaggct
    16021 gatacttggc cttctttgta agataaaagg ccttaacatc aggatctgcg gatatgaagc
    16081 tttcatcgca tttgaagttt tcggccatta tctgcctggc tgcctgcacg tcacagatgt
    16141 agtaataggg agatgcgtca taagtcgtac caggcatttg gatgaaatta acgttctcta
    16201 tcttgaattt cgtaatataa gaagtaagct taacagtctc gttaagtgtc agattggtct
    16261 gtacatgctc gaacatggta ttgataacgc tggtaagctt cggcaggtac tgcagcgtca
    16321 gcttttgcct catcaactcc cttatgaact gctgctgggc ctcatttctt ttcaggtcgc
    16381 tgccgtcgta gtatttcctg atctctttgt tccacttgtt gggtttacgg aacctcacat
    16441 actgttcggc ctgttcgcca taaagccttt gctgcccttt tttgaggtgt atgtggagat
    16501 tctgtacagg atcgtcataa tccatatcta cgggtacata atagtcaaca ccgtccagca
    16561 aatcgattat tttcctgaag gctgaagtat ctacaaaagc gtaatacttg atattgacat
    16621 ccagcaattc ggatacggtc ttggctgaaa gttcgacacc cccgttaggg aaagcatagt
    16681 tgatctttct ttccttattg tttataaaaa ccctcgtatc cctgggtatc gacatgatgt
    16741 ttattttgca tgtttcaggg tcgaaattgg caagcatcat cgtatctgtc aggttgttga
    16801 ccttgtcgcc gccaaggaga agaatattaa agggttcctt tttcgttacc aggggattga
    16861 ttatatgggc caggtggttg tcctggtcgc ctgatgcaaa aacatcactg cttataatct
    16921 ctatattggt gttgatgtag taaagtattc ctgagccaaa aacgaataaa acaactgctg
    16981 cgactatcgt caatataa
//
LOCUS       DAOSCC010000022        16303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2454_length_16303_cov_4.892110, whole genome shotgun sequence.
ACCESSION   DAOSCC010000022 DAOSCC010000000
VERSION     DAOSCC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_16303_cov_4.892110"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..149
                     /locus_tag="PLG72_02350"
     tRNA            65..149
                     /locus_tag="PLG72_02350"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Tyr,seq:gta)
     gene            complement(362..1756)
                     /gene="murD"
                     /locus_tag="PLG72_02355"
     CDS             complement(362..1756)
                     /gene="murD"
                     /locus_tag="PLG72_02355"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOJ79677.1"
                     /translation="MNTKLEQFKKEIVNKRVAVLGIGISNTPLIRYLGKLGVEVTAFD
                     KAGKDDLIPALKTLEGLNIEYSLGPDYLEKLRGFDVIFKTPKIRFDIPELLREAEAGA
                     EITSEMEVFCRLCPARIFGITGSDGKTTTTTLIYKILEHSGYKCWLGGNIGTPLLDRI
                     DEIDESDMVVLELSSFQLHTMRNRINTAVVTNLSPNHLDVHKSMQEYIDAKKNIFLYQ
                     NENDTLVLNYDNDITRGFASEAQGKIIMFSRTNGLDEGMALSGGKIVYRHGGRDQEIV
                     DASDIRIPGVHNIENYMAAAAATLDFAKPSDVRAVAMSFGGVEHRNEHVRTVNGVSFY
                     NDSIGSSPTRTMATIRAFNDKVILISGGYDKHLSYDEMGEVLAERVKCLVLFGQTSDK
                     IEAALKAEVQRSGRGADIPVFRCTSMEEAVRKAYENAVPGDVVLLSPASASFDHYRNF
                     EERGNHFKKIVMVL"
     gene            2004..2090
                     /locus_tag="PLG72_02360"
     tRNA            2004..2090
                     /locus_tag="PLG72_02360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2038..2040,aa:Leu,seq:cag)
     gene            complement(2256..3200)
                     /locus_tag="PLG72_02365"
     CDS             complement(2256..3200)
                     /locus_tag="PLG72_02365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79678.1"
                     /translation="MERTMAYMRKAYRDEDFIRIRDFLKYTLYENPSRKNWLIDRWNF
                     GRYFSQTMHGTFDTWPETVGIWEDENNEIIAVVNSEGEVINRKAGSAFFQLGNRDFTD
                     DFLSELIDYAESRLALTTEEGTYVNVFVDEDAFSIRKLLKEKDYVLLEWKDPMSCMDI
                     SGCLKVELPEGFRIADANEVSDCQKGFAHGRAFGYYINDAPDDDAAERCFQALRNAPD
                     YLPELDLSILDRNGEVASFAGVWYDDLNRIGILEPVGTIPKYRRMGLGKAVVYEGINR
                     VLKMGANRIFVGSDQQFYLSIGFRAIYYKQIWQKKLRD"
     gene            complement(3112..3975)
                     /locus_tag="PLG72_02370"
     CDS             complement(3112..3975)
                     /locus_tag="PLG72_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOJ79679.1"
                     /translation="MIHLIRKIRNNAHDGSERLFAAEGLWAHKLLIETGSAIRCVVIC
                     PELLYSAEALELAESCLDKASAAYIVSGKLFEKISERDGPDGLISLVQLPVYKPESLQ
                     IKEKALIVVLDGLENPGNIGTILRTCDGAGADAVFICNRKARMTNPKLIKSSMGAVFT
                     VPVIEFDDTGLCIDWLKARNFSIYLADAKAGRTYRDFDYTGNTALVMGSERYGISEKW
                     YSCDPQPISIPMMGICDSLNVGAAASVIIYEISMKRLQKGYGANNGIYEKSVQGRRLY
                     QDKGFSEVYFI"
     gene            complement(4202..4465)
                     /locus_tag="PLG72_02375"
     CDS             complement(4202..4465)
                     /locus_tag="PLG72_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HOJ79680.1"
                     /translation="MKYIKAEEILPEKLLREIQDYVCGELIYIPSLEGMRKGWGEKSG
                     TRDYLRKRNMEIRTRFREGMSIDQLSEIFCLSCDSIKKIVYAK"
     gene            complement(4593..4742)
                     /locus_tag="PLG72_02380"
     CDS             complement(4593..4742)
                     /locus_tag="PLG72_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79681.1"
                     /translation="MCRIKLMDAVSLALRLPYIAKSSKGVVLMAELIIMKGFLGKKLH
                     ARSAG"
     gene            complement(4735..4911)
                     /locus_tag="PLG72_02385"
                     /pseudo
     CDS             complement(4735..4911)
                     /locus_tag="PLG72_02385"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4926..5339)
                     /locus_tag="PLG72_02390"
     CDS             complement(4926..5339)
                     /locus_tag="PLG72_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOJ79682.1"
                     /translation="MWKCPKCGREFKKEGQHHFCGDASTMTVDEYIEAQLPEIRQILD
                     KVRETIRKVLPDAEERIAYGMPTYRGKRNIIHFAAQKKHLGLYPGDKAIEHFADRLSG
                     YKTSKGAVQFPYDRPIPLDLITEIAQWCRDTGSHH"
     gene            complement(5427..5902)
                     /locus_tag="PLG72_02395"
                     /pseudo
     CDS             complement(5427..5902)
                     /locus_tag="PLG72_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(6057..7292)
                     /locus_tag="PLG72_02400"
     CDS             complement(6057..7292)
                     /locus_tag="PLG72_02400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ79683.1"
                     /translation="MGRSGKKVIFLVDMNAFFISCEMSINPELKGRPAAVAGDPKSRS
                     GIILAANYEARAYGVKTTMLVHEALSICPGLVIVPPTRGLYEKKSNEFMEILSRYAPV
                     IQKNSIDEAWLDLTGCEKLFGEPVQIARRIMREIQYELDLWCSIGISENKFLAKMASE
                     MKKPLGITELWKEDVPVKMWPLEIRQMYGIGKKTEERLKRIGIMTIGDLAQCDDRLLN
                     GNFGKYGQELKRLANGIDDSPVEPEPKSVTRSISRSTTLAKDITDINEARTVLLSLAE
                     EVGEEARRSDFRGKSVSIVIRYNDFTTITRQKTIVPSFLTKDIYRTGAELLGEHWDRK
                     RPIRLLGIGISNVDEEMPVQLSIFDINDELPDSVKREEKLERAIDSIRSRFGSDKLKR
                     ATLVEGRKKNTGETAGVSE"
     gene            complement(7294..8070)
                     /locus_tag="PLG72_02405"
     CDS             complement(7294..8070)
                     /locus_tag="PLG72_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ79684.1"
                     /translation="MDYEGFKQEILKMSDIDLSLYKEKQMKRRIDSLIRKNGYDRYDD
                     YVHALQQDRELYNEFINYLTINVSEFYRNPDQWEVLRDQIIPMLLQRSPMPRVWSSAC
                     STGDEPYTLAMVLSEHMPLNRIHILASDIDAEALKKAKAGVYSAKSLEKLPERYIREF
                     FIKDGNSYHVRDELKRCVEFKRINLLRDPYPNECDLIVCRNVLIYFTEEAKSQIYVKF
                     NKALKDKGVLFIGSTEQIIMSARYKLTPVKTFFYIKDGDI"
     gene            complement(8181..10250)
                     /locus_tag="PLG72_02410"
     CDS             complement(8181..10250)
                     /locus_tag="PLG72_02410"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOJ79685.1"
                     /translation="MEIILAKTAGFCFGVNNAIRIAEDMLGKGGAISTLGYLIHNDQV
                     VNDLKEKGIRAISCISEAPEGSKVIIRAHGVAPGIYEEAKKRNIEIVDATCPYVKKIH
                     KLVEKMHKDGYTIVIAGDREHPEVIGVNGWCGNSALIANTPEEAEAFAPIDGKCCLVA
                     QTTITRERFDGIYDKLKEKCKSVVKFDTICSATSSRQEEAEKIARISDVMLVIGSRKS
                     SNTRKLYEICSKYCKKTYLIETYGDLPPINTKEVQTLGITAGASTPERTIKEVIEKMD
                     ELNKQEKEMSFEEAFENSMITLRSGEIVKGRIIGYNNAEIFVDLGYKSDGIIPVEEFS
                     DDPDFKPETGVNIGDEVDVFIVRVNDGEGSVMLSKKRVDSMRSVEMIEEAYKNKTPVT
                     GKVVEVTNGGVIVSVGGVRVFVPASQLSERYVKDLGEFLKQTVTIRIIEMNRQKRKFV
                     GSRKVVLAEEREQKEKQVWSNIEVGKKYTGTVKSIMDFGAFVDIGGVDGLVHVSEMSW
                     IKVKHPSELVKVGDKVEVTVLEFDPEKKRISLSMKKEEDNPWFRVSEKYKVGDIVKGT
                     VVRLVPFGAFIELEKGLDGLVHISQISSVRIAKPGDVLKTGQEVEAKIIEVDEEAKKI
                     SLSIKEVNPIYPEPAVREQKAPEKAQDEAQKEKADTEAEEEIPKEHKEDLNNTIGDVI
                     GSLDEIK"
     gene            complement(10251..10832)
                     /locus_tag="PLG72_02415"
     CDS             complement(10251..10832)
                     /locus_tag="PLG72_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ79686.1"
                     /translation="MRKFLRILTNIVFKIFFKVEVIDYHKVPEEGPALLCASHNTMID
                     MFFLGFKLKRWIYWMAKEELFRNPLFACILKKLGAFPVRRGTADISSIKHAYKLLREN
                     KIVGIFPQGTRTRTAKRKYPAKSGTVLIAANAGVPVIPATVCGTYRLFSKMKVVYGDP
                     YYIEKKGDKLPKEYLSEMSKDMMRRIDALSEVR"
     gene            complement(10829..11512)
                     /gene="cmk"
                     /locus_tag="PLG72_02420"
     CDS             complement(10829..11512)
                     /gene="cmk"
                     /locus_tag="PLG72_02420"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ79687.1"
                     /translation="MINIAIDGPAGAGKSTVAKAISKELGIIYLDTGAMYRTVALKAI
                     RSGIDTGDKEAIESMVKDIDIRIEYKDNEQRIYLDGSDVTGFIRTPEVSVGASDVAVI
                     PAVRKKMVELQRKIAKENDVVMDGRDIGTYVLPDASYKFFLTASLDERAKRRFNEMKA
                     NGMTGVSLEEVKKDIEYRDKNDSSRSFAPLAKAPDALEIDTTGISPEEVVRIIMDSIK
                     NRKTVGAAK"
     gene            complement(11521..11901)
                     /gene="aroH"
                     /locus_tag="PLG72_02425"
     CDS             complement(11521..11901)
                     /gene="aroH"
                     /locus_tag="PLG72_02425"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOJ79688.1"
                     /translation="MVRAIRGATTVKDNTREEIFSATTELVSEIIEQNGLTRDDIISI
                     IFSVTSDLDAAFPAAAVRQMGFDRTAMLCTYEMDVPGSLKKCIRVLMHVETGKRNDEL
                     KYIYLKEAKKLRPDIVQQNGRMED"
     gene            complement(11908..12333)
                     /locus_tag="PLG72_02430"
     CDS             complement(11908..12333)
                     /locus_tag="PLG72_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HOJ79689.1"
                     /translation="MEQTVKEYGSRDIAAAAVKIALTKDRTEEKQLQQELARLGIRTA
                     AVDYGGEFINSVMKIIERAVVSSKREGVIQESHVEEGAVAGATREALSQIMPKALGLN
                     VGGKIGIARYRNHISVSIFFGIGLLHLNEVAIGLGHRVV"
     gene            complement(12440..12937)
                     /locus_tag="PLG72_02435"
     CDS             complement(12440..12937)
                     /locus_tag="PLG72_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79690.1"
                     /translation="MKTIISKETDNSSPGKGNNVRICDGIEKILRNIFIIVFVIFITI
                     QAVLTDDGIRGLLVRDALDGKPIGDEVFLFEKCKMELRLKGMPNCPELKVLVNGVEAG
                     AFNGNAVLLELKEGDVVELDASRLLVNTEVQITAVSRNLAHILGRTVSAADGIVPVAV
                     ADTDE"
     gene            complement(13003..14232)
                     /locus_tag="PLG72_02440"
     CDS             complement(13003..14232)
                     /locus_tag="PLG72_02440"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ79691.1"
                     /translation="MRRRVIVIGAGAAGLMAAGKAAERGLDVLVLEKNDKPGRKLRIS
                     GKGRCNITNDTDIDGLIRNIPGNGSFLYSSFYTFSNEDIMQFFTDEGLELKVERGGRV
                     FPVSDDAKDVVSVLVRHAVESGAEIRYRSPASSIEVSNGAVKGVKLKNDETIECDSVI
                     LATGGESYPGTGSTGDGYRMAQKLGHTVTPLRPSLVPLVSSEKWVRDLQGLTLKNISI
                     TLYDRQRKKIYNDFGEMLFTHFGVSGPVILSASRHIMQYDYRNVFLSIDLKPALSPEV
                     LDSRIQRDFEKYSRKQFKNSLDDLLPKKMIPVIVELSQIPADKPVNQITKAERRSLAS
                     LLKGLTLEICGSRPISEAIVTAGGVSTDEIDPATMESKLVKGLFFAGEVIDVDGYTGG
                     FNLTIAFSTGCVAGLNC"
     gene            complement(14229..15125)
                     /locus_tag="PLG72_02445"
     CDS             complement(14229..15125)
                     /locus_tag="PLG72_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOJ79692.1"
                     /translation="MDNKSFDLINRINSIKKLSKGQKLIVDYITHHYDKAAYMTAAKL
                     GEAVGVSESTVVRFALELGYEGYPKMQKVLQEMVKNKLTAIQRMEISSSRIDEYNILK
                     SVLQSDMEKIKITMEEIDYEAFNATVECILNARHVYILGVRSSAPLAYFLGFYLNLIQ
                     DNVRLVHTNSISEMFEQIIRASKGDVVIGISFPRYSKRTAKAMEFVKKQGAAVIAITD
                     STESPVAGNADHCICARSDMASFADSLVAPLSVINALIVAIGMRKKKSLYETFEKLEK
                     IWDEYQVYEKYDLDEPDRKVDY"
     gene            15301..15978
                     /locus_tag="PLG72_02450"
     CDS             15301..15978
                     /locus_tag="PLG72_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79693.1"
                     /translation="MIQIDDSGSGSFVGGTCIGVYRPETNEYYFDIIPVELYNGENFA
                     QKKYLDYVVNIVKSAFMALKPAKGEMIEICRGYMFDRLKAWLTANGYLWYSTQITGRV
                     QEIVEKCFELYAERLGLPGHYIKYTRYPFHFHKLLRWVYADFDNRIRLCKVGWKCWQK
                     IAGQVPDVSDACVCVPSFICMKCGRYIRSGSEISVIRFVSNRENYVYVHKKCRIKHGS
                     CHREFSV"
     gene            complement(16064..>16303)
                     /locus_tag="PLG72_02455"
     CDS             complement(16064..>16303)
                     /locus_tag="PLG72_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400767.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOJ79694.1"
                     /translation="IARKVLGDREPITCRPADLIEPELEKIRKQIGQYIEQDEDVLSY
                     ALFPQVAENFFKQRQAAKYKVDGTMVDYEFRVHPV"
BASE COUNT         3591 a         4393 c         3405 g         4914 t
ORIGIN      
        1 gagcaaccgg ctcataaccg gttggtccgg ggttcgagtc cctgaaggcc caccagttca
       61 atacggaggg atacccaagc ggccaaaggg ggcagactgt aaatctgttg tcgatgactt
      121 cgatggttca aatccatctc cctccaccaa aaagaaaacc tcctggtacc cgggaggttt
      181 tcctttttat gcgccgggca ctccggccgg tcagttgctc aggacatgga acggttccag
      241 tatccggcag gtgtcatttt ccgggaccga atacagttct cctgatttgg tgccaatcta
      301 cgcgcttgtg aggcgcggtg acggtacgtc tcttccgcac gccccgtgcc tggcgccatt
      361 cctacagtac catgacgatc ttcttaaagt ggttccctct ttcctcgaag tttctgtaat
      421 ggtcgaagct tgcgcttgcc ggcgagagca gcacaacatc cccgggcacg gcattctcat
      481 atgcttttct taccgcttct tccatcgaag tgcagcggaa gaccggtatg tcggctcctc
      541 tgcccgacct ttggacttcg gctttcagcg cagcttctat cttgtccgaa gtctgtccga
      601 acaacacaag gcacttcacc ctttccgcca ggacttctcc catttcatcg tatgaaagat
      661 gtttgtcgta acctcctgaa atgagtatta ccttgtcatt gaatgccctg atcgttgcca
      721 tggtccttgt tgggctggaa ccgatggaat cgttgtagaa acttacgcca ttgacggtcc
      781 tgacatgctc gtttcggtgt tccaccccgc cgaatgacat ggcgacagcc ctgacgtccg
      841 aaggttttgc gaagtccagc gttgcagcgg cagcagccat ataattttcg atattgtgga
      901 caccggggat cctgatatca ctggcatcga cgatctcctg atccctgcct ccgtgcctgt
      961 aaactatttt gccgccggac agcgccatgc cttcatccag gccattcgtg cggctgaaca
     1021 tgatgatttt tccctgggct tccgatgcaa agcctcgggt gatatcgttg tcataattca
     1081 ataccagtgt gtcgttttca ttctgataga ggaagatgtt ctttttggca tcgatgtatt
     1141 cctgcatcga tttgtggaca tccagatggt tcggcgaaag attcgtcacg actgctgtat
     1201 ttatcctgtt cctcatcgtg tgcagctgga aactgctcag ttccaggacc accatgtcgg
     1261 attcgtctat ctcatctatc ctgtcaagca gcggcgttcc gatattgcca ccgagccagc
     1321 atttgtaccc gctgtgttcg agaattttat atatgagcgt ggtcgtggtg gtcttgccgt
     1381 cgcttcctgt tatgccgaag atccttgccg ggcacagcct gcagaacact tccatctccg
     1441 aagtgatctc agcgccggct tcggcttccc gaagaagttc cggtatatcg aagcgtatct
     1501 tcggtgtttt gaatattaca tcaaagcccc tgagtttctc caggtaatcc gggccgaggc
     1561 tgtattcgat attcaggcct tccagcgttt taagcgccgg tatcagatcg tcctttccgg
     1621 ctttgtcgaa ggctgtgact tcgacgccga gcttgccgag atacctgatc agcggcgtgt
     1681 tgcttattcc gatgccaaga acggcaactc tcttattcac gatttccttt ttgaactgct
     1741 caagcttcgt attcaatcca gtatacctcc aaactgctaa aagcattata acgcatcaaa
     1801 gaagaacttc cgactgcata aaactttctt gcctctgcgc ttctcaaaca ggcgggagat
     1861 atgctcaccg cctaaaaggc agatcggtac cccgccgtaa atagagggtg gacatctgtc
     1921 tccttgtaat aaattttata aattaccact tgcaattata ctacatttct agtagaataa
     1981 atattgcgtt ttgaaaagcc cacgcgggaa tggcggaatt ggcagacgcg ctagtttcag
     2041 gtactagtga gctgaagctc atgcaggttc aagtcctgtt tcccgcacca gtagaaaaag
     2101 agatcgcggt aaacgtgatc tcttttttac atccttagct ggtttgatgt ccggaacgtc
     2161 aggtaggact cagggacgtt acccgggtcg tgatcccgca cgattcgggc atgggaaccg
     2221 tccccgtcgc tgcagaaacc gttcttctgt cttcttcagt cacgcagttt cttctgccag
     2281 atctgcttgt agtagatcgc cctaaagcct atagaaagat aaaactgctg gtctgaaccg
     2341 acaaagatcc tgttcgctcc cattttcaga acccggttta tcccctcata aacaacggct
     2401 tttccgaggc ccatccttct gtacttcggt atcgtcccaa ccggttccag tatcccgatc
     2461 ctgttcagat catcgtacca gacacccgca aaagaagcaa cctcgccatt cctgtcaagg
     2521 atcgacaggt ccagttccgg caaataatcc ggcgcatttc ttaatgcctg gaaacagcgt
     2581 tcggcagcat catcatcggg agcatcgttt atataatagc caaatgcgcg tccatgggca
     2641 aagccttttt gacagtcgct gacttcattt gcgtccgcga tcctgaatcc ttcgggcagt
     2701 tccaccttga gacagcctga aatatccata caggacatcg gatctttcca ctccagaagc
     2761 acataatcct tttctttcag tagtttccgg atactaaacg catcctcatc gacaaacaca
     2821 ttgacgtacg ttccttcttc tgttgtcagt gccaacctgc tttctgcgta atctatcagt
     2881 tcactcagaa aatcatcggt aaaatccctg tttcccagtt ggaaaaatgc actgcctgcc
     2941 ttcctgttta tgacttcacc ctcagagttg acaactgcaa tgatctcatt attttcatcc
     3001 tcccagatac cgacagtttc aggccaggta tcaaaagtgc catgcatggt ctggctgaag
     3061 tatctgccga aattccatcg gtctatcagc cagtttttcc gggacgggtt ctcatataaa
     3121 gtatacttca gaaaatccct tatcctgata aagtcttcgt ccctgtacgc ttttctcata
     3181 tatgccattg ttcgctccat atcctttctg caatcttttc atgcttattt cgtaaatgat
     3241 caccgaggct gcagcaccaa cattcaggga gtcgcatatc cccatcatcg gtatggatat
     3301 cggctgcgga tcgcatgaat accatttttc ggatattccg taccgctcgc tgcccattac
     3361 gagtgcagtg ttacctgtat agtcgaaatc cctgtatgtt ctccctgcct ttgcatctgc
     3421 caaataaatg ctgaagttcc tggccttcag ccagtcaatg cacagtcccg tgtcatcgaa
     3481 ttctatgaca gggactgtaa aaacagcgcc catactggat tttatgagct tcggattggt
     3541 catccgggct ttcctgttgc aaataaaaac tgcatctgcg cccgccccgt cacaggtacg
     3601 gaggatagta ccgatattcc ccggattctc caggccatcc agtacgacta tcaacgcttt
     3661 ttctttaatt tgcaggctct caggtttgta taccggcaac tgcaccagcg aaataagtcc
     3721 gtccggtccg tccctttcgc tgatcttctc aaaaagcttt cctgatacga tatatgcagc
     3781 ggaggcctta tccagacagc tttctgccag ttccagcgct tcagcgctgt acagcagttc
     3841 aggacatatg acaacacagc ggatagctga acctgtctca ataagcagct tgtgagccca
     3901 cagcccttcc gcggcaaaaa gcctttcgga tccatcatgg gcattgttcc ggatttttct
     3961 gatcagatgg atcaccggat gctgccgtcc aatatacctg atttttcttt tatgcttgcc
     4021 gagaatctta tctcttatgt cgccatacat tacgatcagc ccgctcttgt tcgttcctga
     4081 tataataata agggaatcag caggccgttt atatataaaa aacgccttaa cctatttatt
     4141 gcacaaaccc gacgggttcg ccgggtttgc atatgacact gttctgtttt tcctctgtgg
     4201 gtcattttgc atagactatt ttctttatac tgtcacatga gagacagaat atttctgaaa
     4261 gctggtcgat agacatacct tcgcggaacc tggtcctgat ttccatgttt cttttcctga
     4321 ggtaatccct ggtcccggat ttttctcccc aacctttgcg catgccttca aggcttggga
     4381 tataaataag ctcgccgcat acataatcct gtatttccct gagcagcttt tcaggcagga
     4441 tttcttcagc ctttatgtat ttcattccaa ctccgctcct taaaattttc ctgattttaa
     4501 ggatgcaaag cccgcgatat aaacggctca tctgaagtca gaatgaacgt ggcgatcatt
     4561 tgttcggctc catgcaatca atcgccactc gtttatcctg cggaccttgc atggagcttt
     4621 ttgcccaaga aacccttcat tattatcagt tctgccatta ggaccactcc tttcgaactt
     4681 ttcgctatgt atggaagccg caatgccagt gatactgcat ccatcagttt tattctacac
     4741 atcaggacca tttcctgcaa tgatttccgt aatcccctgc atcggagcat gcatatacct
     4801 ttcaagccgt tcgatgaccc tgcttttgcc cattatctgc attatctcat aaagatccgg
     4861 ggtattcgta cggcctgtca gtgcgatcct caggaccatc gccacatcgc cgacatggca
     4921 gcccgttaat gatggctccc ggtatcacgg caccattggg cgatctcagt gatgaggtcc
     4981 agcggtatcg gcctgtcata cggaaactgc acggcgccct tgctggtttt atagccggac
     5041 agtctgtccg caaaatgctc aatggccttg tcgccgggat aaaggcccag gtgctttttc
     5101 tgcgcggcaa aatggatgat gttgcgttta ccacggtaag tcggcatgcc atacgcgatg
     5161 cgctcctccg catccggaag gactttgcgg atggtttccc ttaccttgtc aagtatctgc
     5221 cttatttccg gtagctgcgc ttcaatatat tcatcgaccg tcatcgtcga cgcgtcgccg
     5281 cagaaatggt gctggccttc ttttttgaac tcgcggccgc atttcgggca tttccacatg
     5341 aatgaaccac cctttgcctt aacttgatag atattataac atacatattc ggatatgaat
     5401 gccatcagcc gggtctgaaa atcatatcag gctttgcagc atttatttat gaagtccatc
     5461 tcataacgga cagtgccgtg atcttcgagt actgtcgcac tggtgatacg gatcgcaaga
     5521 ataatgcagt tttcatcatt ttcattattt gcgtcgtcat accattcggc aaaagcttcg
     5581 cggagcttag ccctcacgcc ggcatttttg gggtccagga cccaaccgag attttcaccg
     5641 atgccgtttc ccgatatcca cttaccgctg acagcaaacg caacttccgg gttttgggct
     5701 atctgctgca tttttgcaga ttttgcccat gtagtgatat agaacacacc attttcataa
     5761 agcgcatcca cttcacggac acgcggacgc ggtttcccct ctgcattctc ttctgtagta
     5821 aggtagccag ggctatgata ttatccttcc cgttgccgca cctttcatcg aatattttca
     5881 acccttcttc aaacctgtcc attttgatct ccccctgtac cacttttttg cctggtgttt
     5941 tcatgcggct gtttgccggt cagcttggcc gtatggatat ttacattata ccataatctg
     6001 tgatcaattc aggaatgcct ggatgattca gagcagttgt tagtaacaac tgatttttac
     6061 tcactgacac cagctgtctc acctgtgttc ttttttcttc cttccactaa cgtcgccctt
     6121 ttcagtttgt cgctgccaaa acgggacctg atactgtcta tcgccctttc gagcttttcc
     6181 tccctcttta cggaatcagg cagttcatcg ttgatgtcga atatgcttag ctgtaccggc
     6241 atttcctcat ccacattcga gatgcctatg cccagaagcc ttatcggcct cttccggtcc
     6301 cagtgctcac caagcagttc cgctccggtg cggtatatat ccttggtgag gaaagaaggc
     6361 actatggttt tctggcgggt gatcgtagta aagtcgttgt aacgtatcac tatggatacg
     6421 gatttccccc tgaaatcact tctcctcgcc tcttccccga cctcttccgc aaggctgaga
     6481 aggaccgtcc tcgcttcatt aatatctgtt atatcttttg caagtgtggt cgacctgctt
     6541 atcgacctgg tgacgctttt gggttccggt tccaccggcg aatcgtcgat cccgttggcc
     6601 agccttttta gttcctgccc gtatttcccg aagttcccgt tcagcagtcg atcgtcacat
     6661 tgcgccaggt caccgatggt catgatgccg atccgtttca gcctttcctc ggttttcttc
     6721 cctatcccgt acatctgcct tatttccagc ggccacatct tcaccggcac atcttctttc
     6781 cagagttctg ttatgcccag cggctttttc atttcagacg ccatcttggc aaggaatttg
     6841 ttttccgata tccctatcga gcaccagaga tccagttcgt actgtatttc cctcatgatg
     6901 cgccgggcga tctgaacagg ctcgccgaaa agtttctcac agcctgtcag gtcgagccat
     6961 gcttcgtcaa tgctgttttt ctggataaca ggagcatatc gcgacagtat ttccatgaat
     7021 tcgtttgact ttttctcgta aaggcccctg gtggggggta ctatcaccag gccggggcat
     7081 atcgacagcg cctcatggac aagcatcgta gttttgacgc catatgccct ggcttcgtaa
     7141 tttgcagcca gtatgatacc cgacctgctt ttcgggtcgc ctgccaccgc ggccgggcgg
     7201 cctttcagtt caggattgat tgacatctcg catgatatga aaaatgcgtt catatcgacg
     7261 agaaatatga cctttttccc agacctgccc atttcagata tcgccgtcct tgatatagaa
     7321 aaacgtcttt acaggcgtca gcttatatcg tgcggacatt atgatctgtt ctgtgctccc
     7381 tatgaacagc actcccttgt ctttcagcgc tttattgaat ttgacgtata tctgggattt
     7441 tgcttcttcg gtgaaatata tcagcacatt cctgcatacg ataagatcac attcatttgg
     7501 atacggatcc ctcagaagat taatgcgttt gaattccacg caccgtttaa gttcgtccct
     7561 gacatgatac gaattcccgt cttttataaa gaattccctt atatacctct caggcagctt
     7621 ttcaaggctt tttgccgaat acacgccggc tttcgccttt ttcagcgctt cggcatcgat
     7681 atctgaagcc agtatatgta tcctgttcag cggcatatgt tcactcagca ccatggcaag
     7741 ggtatacggc tcgtctcctg tcgagcatgc cgaactccac actctcggca tcggcgatct
     7801 ctggaggagc atcgggatta tctggtccct aagcacctcc cattggtccg gatttcgata
     7861 gaattcagac acattgatgg taagataatt gatgaactca ttgtatagtt ccctgtcctg
     7921 ctgcagtgca tggacataat catcatatcg gtcatagccg tttttcctga tgagcgaatc
     7981 tatccttcgc ttcatttgtt tctctttata caggctgagg tctatgtctg acattttcag
     8041 gatctcttgt ttaaaccctt cgtaatccat gaccggtccc ccgaatcaaa ttataaaatg
     8101 aaataaaatg agtaaactga aaatgcaaga catatgtcct gcattttcaa gatgtcaaat
     8161 cgtcggtcaa ttttttcgat ctatttgatt tcgtcaagcg atccaatcac atcgccgatc
     8221 gtgttgttga gatcttcctt gtgctctttt gggatctcct cttcggcttc cgtatctgct
     8281 ttctctttct gtgcctcgtc ctgtgccttt tccggggctt tctgctccct taccgcaggt
     8341 tcgggataaa tgggattgac ctcttttata gaaaggctta tcttcttcgc ctcttcatct
     8401 acctcgatga tctttgcttc cacttcctgc ccggtcttga ggacgtctcc aggcttcgcg
     8461 atccttacgc tcgatatctg tgaaatgtgt acgagcccat ccaggccttt ttctagctcg
     8521 atgaacgcgc cgaacggcac aagccttacc acggtcccct tcacgatatc tccgaccttg
     8581 tatttttcgg atacccggaa ccacggattg tcctcttcct tcttcatgct cagcgatatc
     8641 ctctttttct cgggatcgaa ttccagtacg gtgacttcga ctttgtcgcc taccttaacc
     8701 agttccgagg gatgcttgac ctttatccat gacatttcgg aaacatgaac aaggccgtcg
     8761 actccgccaa tatcgacgaa tgctccgaag tccataatgc tctttacggt accggtatat
     8821 ttctttccga cttctatatt cgaccagacc tgtttttctt tctgttccct ttcttcagcc
     8881 aggacaacct ttcttgaacc gacaaacttc cttttctgtc tgttcatctc tattatcctg
     8941 atagttacgg tctgtttaag aaattcgccg agatccttta catatcgttc ggataactgg
     9001 gaagcaggta caaataccct gacgccgccg acactgacta tgactccgcc gttggtaact
     9061 tcgacgactt tgccggtcac aggggtcttg tttttatatg cttcttctat catctcgaca
     9121 ctgcgcatcg agtctactct tttctttgaa agcattacgc tgccttctcc gtcgtttacc
     9181 ctgactatga atacatcgac ttcatcgcct atatttacgc cggtttcagg tttgaaatcg
     9241 ggatcatccg agaattcttc gacgggaatg ataccgtcag atttatatcc cagatcgacg
     9301 aaaatttctg cgttgttgta gcctatgatc cttcccttga cgatctcacc gcttctcagc
     9361 gtaatcatcg agttttcgaa tgcttcttcg aaactcattt ccttctcctg tttatttaat
     9421 tcatccattt tctctataac ctccttgatt gtgcgttccg gtgtagatgc tccggctgtt
     9481 atccccagcg tttgtacttc ttttgtattt atcggcggca aatcaccgta cgtctctatt
     9541 aaatatgttt ttttgcagta tttactgcat atttcataca atttacgggt gttggagctc
     9601 tttctgctcc ctatcaccag catgacatcc gatatgcggg ctattttttc agcctcttcc
     9661 tgtcggctgc tggtcgcact acatattgta tcaaatttga cgacactttt gcatttttct
     9721 tttagtttat catatattcc atcgaacctt tcacgcgtaa tcgtggtctg ggccacaagg
     9781 cagcatttcc cgtcgatggg tgcaaaagct tccgcctctt caggcgtatt cgctataagc
     9841 gcagagtttc cgcaccatcc gtttacccct atgacttcag gatgttccct gtctcccgct
     9901 attactatcg tataaccgtc cttatgcatt ttctcaacca gcttgtggat ctttttaaca
     9961 taaggacatg tcgcatcaac tatttctatg ttccttttct tcgcttcctc atatatcccg
    10021 ggtgcgactc cgtgcgccct gataattacc tttgatcctt caggcgcctc gcttatgcat
    10081 gatatcgctc gtataccctt ttccttcagg tcgttaacta cctggtcatt gtgtatcagg
    10141 taaccaaggg tactgatggc gcctcctttg cccagcatat cttcggctat cctgatcgca
    10201 ttattcactc caaagcaaaa acccgcggtt tttgccagaa ttatctccat ttacctgacc
    10261 tccgacaggg catctattct cctcatcata tctttgctca tctccgaaag atattctttt
    10321 ggcaatttgt cccctttttt ctctatgtaa tacggatcgc cataaacgac cttcattttg
    10381 ctgaatagcc tgtaggtgcc gcaaaccgtt gcgggaatga ccggcactcc tgcatttgca
    10441 gctattagaa ccgttccgga ctttgcgggg tattttcttt ttgccgtcct cgtcctcgta
    10501 ccctgtggaa aaatcccgac gatcttgttt tctctcaaca gtttgtaagc atgttttatg
    10561 gagcttatgt cagccgttcc cctccttacg gggaaagctc ccagcttttt caggatgcag
    10621 gcaaacaaag ggttcctgaa cagctcttcc tttgccatcc aatatatcca tctcttcagc
    10681 ttgaaaccga ggaaaaacat gtcgatcatc gtgttatgac tggcacacag caatgccgga
    10741 ccttcctcag gcactttatg ataatctatg acttcaactt tgaaaaaaat cttaaatacg
    10801 atattggtca agatccgaag aaactttctc atttagctgc accaactgtt ttcctgttct
    10861 taatgctgtc cattatgatc ctgacgactt cttcaggact tatcccggtg gtatcgatct
    10921 ccagcgcgtc aggcgccttg gctaacggag cgaaggatct cgaactgtcg tttttatccc
    10981 tgtattcgat atcctttttc acttcctcca ggctcacgcc ggtcatacca ttcgctttca
    11041 tctcattgaa ccttcttttt gccctttcgt caagcgatgc agtgagaaag aatttatatg
    11101 atgcatccgg caggacatat gttcctatat cgcgtccatc catcacaaca tcgttttctt
    11161 tcgctatctt tcgctgcagt tccaccattt ttttccttac tgcaggtatt acggcaacat
    11221 ccgaggctcc cacagatact tccggcgttc tgataaaccc tgtcacatcg ctgccatcga
    11281 ggtatatcct ttgttcatta tccttatatt cgatcctgat gtcaatatct ttcaccatgg
    11341 attcgattgc ttctttgtcg ccagtgtcga taccggatcg gatagctttt agtgcaacgg
    11401 ttctgtacat ggctcctgtg tcgagataaa ttatgccgag ttcttttgat attgcttttg
    11461 ctactgtact tttcccggct ccggccgggc cgtcgatcgc aatgttgatc atctgtgatg
    11521 tcagtcctcc atacgcccgt tttgctgtac gatatcagga cgcagttttt ttgcttcctt
    11581 caggtatatg tatttcagtt catcattcct cttgccggtc tcgacgtgca tcagcactct
    11641 gatgcatttt ttcaggctgc ccggcacatc catctcgtac gtacagagca tcgccgtcct
    11701 gtcaaaaccc atctgcctga cggctgccgc aggaaacgca gcatccaggt cagacgtcac
    11761 cgagaatatg atgctaatta tatcatctct ggtcagcccg ttctgttcta ttatttcact
    11821 gacaagttca gtcgtggcgc tgaatatctc ttctctcgta ttgtctttta cagtcgtggc
    11881 ccctctgatc gctctgacca ttgtgtctca caccaccctg tgccccagcc cgatggcgac
    11941 ttcattgaga tgcagcagac ctatgccgaa aaatatggat acgcttatgt ggttcctgta
    12001 acgggctatg ccaatcttac cgcctacgtt gagcccaaga gctttcggca ttatctggga
    12061 cagcgcctct cttgtagccc cggcaacggc accttcttcc acatggcttt cctgtattac
    12121 gccttccctc tttgatgaaa ctacggctct ttcgattatc ttcatcaccg agtttatgaa
    12181 ctctccgcca taatcaacgg ctgccgtacg gatacccagc cttgcgagtt cctgctgcag
    12241 ctgtttctct tccgttctgt cttttgtgag cgctattttg acagcagcgg cggcaatgtc
    12301 cctgctgccg tattctttta ctgtctgttc catattcata tcctctcaga acttcttaat
    12361 tattatatag gaataatgga aattgtacaa tattgttatt tacagttatt caattttgcc
    12421 gcgcagccgt ccaaaaagtt cactcatctg tgtcagcgac agccaccggg actatcccgt
    12481 ccgccgctga aacagtcctg cccaggatat gtgcaagatt cctgctgaca gcagttatct
    12541 gtacttctgt atttacgaga agcctgctgg catccagttc gactacgtct ccctctttca
    12601 gttcgagcag taccgcattg ccattgaaag ccccggcctc gacgccgttc acaagtactt
    12661 tgagttcagg gcagttcggc attcctttca gtctgagttc cattttgcat ttctcaaaaa
    12721 ggaacacttc atcaccgatc ggctttccgt ccaaagcatc cctgaccagc aggcccctaa
    12781 ttccgtcatc tgtcagcacg gcctgtatcg ttataaagat gacgaacacg atgatgaaaa
    12841 tgttcctcag tattttttca atgccgtcac atatccgtac attatttccc tttcccggac
    12901 tgctgttatc tgtttccttg ctgatgatgg ttttcatggc tttctccata atttgctaat
    12961 atcgttatta tgtattatgc tcatgcatcc atgaataata catcagcaat tcaggccggc
    13021 aacacagccc gtggaaaagg ctatggtaag gttgaaaccg ccggtgtatc cgtcaacatc
    13081 gatcacttct cctgcgaaaa acaacccttt aaccagtttg gattccattg tcgccggatc
    13141 tatctcatcg gtactgacac cgcctgcagt cactattgct tcgctgatgg gccgcgaacc
    13201 gcatatttcc agtgtcagtc ccttgagcaa tgatgccagc gaccttctct cagccttcgt
    13261 gatctggttc acaggcttgt cagctggtat ttgtgataat tcgacgatca cagggatcat
    13321 ttttttcgga agcaggtcgt cgaggctgtt cttgaactgc tttctggaat acttctcaaa
    13381 atccctctgt atcctgctgt ccagcacttc aggactgaga gcaggcttta agtctatcga
    13441 aaggaagacg ttcctgtagt catactgcat aatgtgcctg ctggcgctca gtatgaccgg
    13501 gcctgacaca ccgaaatggg taaagagcat ttcgccaaaa tcattgtata ttttctttct
    13561 ctgccggtcg tataaagtga tcgatatgtt tttcagcgta aggccctgaa ggtccctgac
    13621 ccatttttcc gaagaaacca gcggtacgag ggacggcctc agcggtgtca cagtatgtcc
    13681 tagtttttgc gccatcctgt agccatcgcc cgttgaacct gtgcccggat aggattctcc
    13741 accagtagcc agtataacag aatcgcattc gatggtttcg tcatttttca acttgacgcc
    13801 tttcactgcg ccgttcgaca cctctatgga actggcaggc gaacggtacc ttatttccgc
    13861 gcccgattcc acagcatgcc ttaccaatac ggataccaca tcttttgcgt catccgaaac
    13921 gggaaaaacc cttcctcccc tctctacttt cagttccagt ccttcatccg taaaaaactg
    13981 catgatatcc tcatttgaga acgtataaaa ggaggaatac aaaaagctcc cgttgccggg
    14041 aatgtttcgt atcagcccgt ctatatccgt atcatttgta atgttgcagc ggcccttgcc
    14101 cgaaatcctc agcttcctgc ccggcttgtc gttcttttcg agcaccagga catcaagtcc
    14161 gcgttccgca gctttgcctg cagccatgag ccccgctgcg ccggctccaa tgacgatcac
    14221 tcttcttctc aataatccac cttcctgtcc ggttcatcaa gatcgtattt ctcatatacc
    14281 tggtattcat cccatatctt ttcaagtttc tcaaaagtct catacaggga tttctttttt
    14341 ctcatcccga tggctactat cagcgcattt attacactca gcggcgcaac aagcgaatcc
    14401 gcgaaggatg ccatatcgct tctcgcgcag atacagtggt cggcattgcc tgcaacagga
    14461 gactcagtac tgtctgtgat cgctatcacg gctgcgccct gctttttgac aaactccatc
    14521 gccttggcag tccttttcga gtaccttgga aaacttatcc caatgacgac gtcgcctttc
    14581 gacgccctta ttatttgctc gaacatctcg cttatactgt tcgtgtgcac cagcctcaca
    14641 ttgtcctgta taaggttcag gtagaagccg aggaaatatg caaggggcgc ggagctcctg
    14701 actccgagga tgtatacgtg cctggcgttc aatatgcatt ccaccgtagc attaaatgct
    14761 tcatagtcta tctcttccat tgtgatcttg atcttttcca tgtccgactg cagcacgctt
    14821 ttcagtatgt tgtactcgtc gatcctgctt gatgatatct ccattctctg tatggccgtc
    14881 agcttgtttt tcaccatttc ctgaagcact ttctgcattt tgggatagcc ttcatatccc
    14941 agttccagcg cgaacctcac cacggtcgat tcacttacgc caacagcctc gccaagtttt
    15001 gccgcggtca tgtacgccgc tttgtcatag tggtgtgtta tgtaatcgac gatgagtttc
    15061 tgccctttgc tcagtttctt aatgctgttt atcctgttta tcaggtcaaa gcttttatta
    15121 tccataaatc ctccgaagaa ataacacact tttggtaacc tgccccaaca atctaatatt
    15181 acattcagca tgcgcaaaaa tcaaggcaac agggatacgt tcgtctcccg gaacgccggc
    15241 gcattttttc agcggtattt gataatgata gaatttgtat gcacttctgt aggaggcagc
    15301 atgatacaga tagacgattc cggaagcgga agcttcgtag gcggtacctg catcggagtg
    15361 tacaggcccg aaacaaatga atactatttt gatatcatcc ctgttgagct ttataacggg
    15421 gagaactttg ctcaaaagaa ataccttgac tacgtggtga atatcgtcaa atccgcattc
    15481 atggccctca aacctgcaaa gggtgaaatg attgagatat gcagaggcta catgtttgac
    15541 aggctgaagg cctggcttac cgcgaacggg tacctgtggt attccacgca aatcacggga
    15601 agagtgcagg aaatagttga gaaatgtttc gaattatatg ccgaaaggct tgggctgcca
    15661 gggcattata taaaatacac gagatatccc ttccacttcc ataagctcct tagatgggtg
    15721 tatgcagact tcgacaacag gatcaggctg tgcaaggtag gatggaaatg ctggcagaag
    15781 atagccggtc aggtacccga cgtatcggat gcctgcgtgt gcgtcccttc gtttatatgc
    15841 atgaaatgcg gcaggtatat caggtccggc agtgaaataa gcgtgattcg tttcgtcagc
    15901 aaccgggaga actatgtata tgtccacaaa aagtgccgga taaaacatgg ctcatgccac
    15961 cgtgaatttt ctgtctgaga catggaaacg gttctcgcgt acggattccg cagaacccgg
    16021 acacaagaac cgtcccctta tctgccagtt ataatattcc ttatcatacc ggatggaccc
    16081 ggaattcgta atccaccatg gtaccgtcga ctttgtattt tgctgcctgg cgctgtttga
    16141 agaaattctc ggcgacctgc gggaacagag cgtatgagag tacgtcctcg tcctgctcga
    16201 tatactgtcc gatctgcttc cttatttttt ccagttcagg ttcgatcagg tcagcaggcc
    16261 tgcatgtgat cggttcgcgg tcacccagca ctttgcgggc aat
//
LOCUS       DAOSCC010000023        15924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2523_length_15924_cov_4.191505, whole genome shotgun sequence.
ACCESSION   DAOSCC010000023 DAOSCC010000000
VERSION     DAOSCC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_15924_cov_4.191505"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLG72_02460"
     CDS             complement(<1..703)
                     /locus_tag="PLG72_02460"
                     /inference="COORDINATES: protein motif:HMM:NF023325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HOJ79695.1"
                     /translation="MKPVGRITVQTVMPQRIFRLRDIAANLWWTWNCEAADMFRMADP
                     SLWEETGGNPAALIKRIGNQRLEQLAGDQDFLKIYDNVVSKFDAYMNRTDTWFSMTYP
                     DLDGHMVAYFSAEYGLSETIPIYSGGLGVLSGDHCKSASDLGIPFTAVGLFYRQGYFN
                     QKINHEGIQETSFSTLNIDELPVSQVTDKNGEPLYISVELPGRTVYASIWQIKVGTVN
                     IYLLDSDVPSNAEQDR"
     gene            925..1326
                     /locus_tag="PLG72_02465"
     CDS             925..1326
                     /locus_tag="PLG72_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79696.1"
                     /translation="MLKRFLFYGLAGWGIEMVWTGMHSLLGGDLTLPGQSNLWMFFIY
                     GCAVFLEPLHDAISGWNWMLRGIVWVIVIWIIEYASGTILYNVLGAYPWYYSDRLSVN
                     GLITLAYGPAWFVAGLIFEKFHRSLDRYGIA"
     gene            2126..>2982
                     /locus_tag="PLG72_02470"
     CDS             2126..>2982
                     /locus_tag="PLG72_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ79697.1"
                     /translation="MPLLIFYGPFEEVKGTIVGASWNTLRHQYIAKFFLSDEAIARIL
                     RDSYADDPTAKGESIEKIELSGEHDSTIEVYNIEGGDFKGKLMVVHDPTRIIVGYSSL
                     FPESGETTSRIAKNNGGVAGINAGGFLDSGWTGTGGIPMGYIIHDGKVVYDQYEGKDV
                     KQETVAFTNEGMLIVGKHAIKQLMEYGVKEGVSFGPPLIVNGKPTIKKGDGGWGIAPR
                     TAIGQRKTGEVLLLVIDGRTIESLGATLREVQDILLEYGAVNAANLDGGSSTTMYYNG
                     KVINRPSDKL"
     assembly_gap    2983..2992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3077..4420
                     /locus_tag="PLG72_02475"
     CDS             3077..4420
                     /locus_tag="PLG72_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79698.1"
                     /translation="MKNIKTIYKWILISVILQTAFLSWLNYVYLPNRGHFKSTMYEAE
                     FLAVKNRSYRLPEGADDISVSYDGLYAAYMLDGQIVIADLDGGREIKRLGPAGGKITY
                     HRWLPDREMLIYAIKEPEGRSGRVRISTHDVIPDLDRSYPDIKGLPEGSAVIDIELSP
                     MTNIVYPKIQTSKTRVRIYRFDIMDNLSLMFKTDLTTVVKETLYSDNLVYQVAGERTR
                     VRNGKTGKTSYIPVKQAHLLLDIDGRDVIYAGITDKSGSVSEIRYGKAGTEAAEWNSI
                     SLSDPAAADDIMITADGSVYIADRQARTVECVKGVEGRKGQTDGSSEKQSGSVGQDGS
                     TGQRNGLSDFKNGLSDWLDGMKEQLNGSKRQNRSSGRNSRSSGQNNISTGQHDVSSGQ
                     NGNASGQNVPASARQGVTAAQDRKLIRYEGRLITMLDQYIVYLDGENLVLRTLEK"
     gene            4530..4859
                     /gene="trxA"
                     /locus_tag="PLG72_02480"
     CDS             4530..4859
                     /gene="trxA"
                     /locus_tag="PLG72_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ79699.1"
                     /translation="MAEGKALALTKENFDEILNGTDKLVVVDFWAAWCGPCRAVAPII
                     EELARDYEGKAVIGKVNVDEQNEISYRYRIMSIPTIMLFKGGQVVDKVVGARTKSELA
                     ELIDKNL"
     gene            4995..5579
                     /locus_tag="PLG72_02485"
     CDS             4995..5579
                     /locus_tag="PLG72_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79700.1"
                     /translation="MAREKRFRTSFMGGFKKSDVNAYIERMLKEFDEKLKSKDDEISV
                     LRNQNRELKSKYEELQQNADEINEARVKIGDVLIQAREKAEAMLEDARAEAAEEKRRL
                     EEANEQEREKLVDIRSDIRNMRTEIVKLLKKYEEQLSEYDGRVTDILKDDAEETADTE
                     TAEAEAATASDHGSGAENAPGYSNNTWNGGSADY"
     misc_binding    5666..5915
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6095..6574
                     /locus_tag="PLG72_02490"
     CDS             6095..6574
                     /locus_tag="PLG72_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ79701.1"
                     /translation="MEEILEILEKNARASVEEIALMLDMSVEEVQAAIRKLEEDKVIV
                     GYNTLINWDNSNKETVTALIELRVTPQRGQGFDKIAERIYRYPQVKACYLMSGSFDLT
                     VIIEGKNMKEVALFVAEKLAPLEGVVSTSTHFVLKKYKNDGVIFEENGKDEREALRL"
     gene            6571..7740
                     /locus_tag="PLG72_02495"
     CDS             6571..7740
                     /locus_tag="PLG72_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ79702.1"
                     /translation="MNLQEMIKPTVRNMPPSGIRKYFDLLNEMKDAISLGVGEPDFVT
                     PWAIREAGIYSLEKGHTHYSSNPGFIELRRQISRYVAKKYNVEYDPKTEIIVTVGASE
                     AIDIALRALGGPGDEVIIPEPSFVAYKGCTMFTGATPVVIELKEEDEFRLKPEQLEEA
                     LTERTKVVILPYPNNPTGAVMRADDQRKIVDVLKDRDVIIISDEIYAELTYSGKHVSF
                     ASYPEIKDKTILINGFSKAFAMTGWRIGYACGPHVLIEAMNKIHQYAIMCSPTMAQYA
                     ASEALNSCEDEVQAMVREYNRRRRVMVKGFRDAGLSCFEPMGAFYVFPSIKSTGMTSD
                     EFCEKLLMEEKVLTVPGTAFGACGEGYIRATYANSYENIAEAMRRIKRFVSRHRK"
     gene            complement(8269..9153)
                     /locus_tag="PLG72_02500"
     CDS             complement(8269..9153)
                     /locus_tag="PLG72_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79703.1"
                     /translation="MLHYIHMKGHISHFSAAMMWNIPYIESVIGDDALGCDSSDITVN
                     SYNARFRINGKKVRSTKLVLPEKSVKTLNGKRVSSPELLFLELANRLDIHRLILLGLQ
                     LCSHPPGNPSLAVTTKQKLEKFVSKTPGHRGHKKALRALKYIENGSASIMESLAYMIL
                     TLPYVLGGYGMRGAIFNYEVKLNNDMSIRLKQDRCFVDLYFKQAKVGVEYDSFAHHNR
                     PSEQGKDSVRSAILGRQGIEILHMNTIQLYDKNACRDFAHNLAARLGKRIHIRTRKFE
                     QMHNHLRTLLPVRSNVCF"
     gene            9700..10801
                     /gene="prfB"
                     /locus_tag="PLG72_02505"
     CDS             join(9700..9774,9776..10801)
                     /gene="prfB"
                     /locus_tag="PLG72_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621577.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOJ79704.1"
                     /translation="MLELEQFKQEIESLGKDIKEMGASLDIARLKLEIEELENKAAEP
                     DFWNDIENSQKVLQRTKTLKTKVETYEKLLSDLEDLKVLNELGIEEQDESVIPEVKQE
                     LERLKDSYSKLKLETLLSGPYDRNNAIMTLHAGAGGTEAQDWVQMLLRMYTRWAEDHQ
                     YEVKTLDYLDGDEAGIKSVTIQIIGENAYGYLRSEKGVHRLVRISPFDASGRRHTSFA
                     SVDVMPELDEEIEVEINPEDLKIDTYRSSGAGGQHVNKTESAVRITHIPTGIVVACQN
                     ERSQIQNRETAMKMLKAKLLELKEREQKEKIEDLKGVQMDIAWGSQIRSYVFCPYTMV
                     KDHRTNYETGDVQAVMDGDLDKFIHAYLSMKQ"
     gene            10848..11894
                     /locus_tag="PLG72_02510"
     CDS             10848..11894
                     /locus_tag="PLG72_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256154.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOJ79705.1"
                     /translation="MVYNKFSDYLKKRYGAKVYKLPVSLSTSCPNRDGTVRMETDAVI
                     RTQTDDGGSRAENYDDTVDCAVDEGSYCGCIFCGIEGSGFETLPAENSIREQLETNMR
                     YIGEKYSAEKFIAYFQNYSNTYLPFDRFSKAMNEACIEGIVALYIGTRPDCIDDRRVD
                     FLKQLKEEKGVDIVIELGLQSVNHHTLRWLNRGHGLAEFIDAVLRIKRAGLEVCVHMI
                     NDLPADDICDVIEAAKVLSALGTDQVKCHSLYILENTVLGRAYKEGRFAPLSMDEFIE
                     RTISFLEYLDPDIVVQRLIGRSPSGRSLFCNWYTSWWKIHDMIVEKMVKEGRYQGKYF
                     DYLNGHALKHFKDV"
     gene            11986..13059
                     /locus_tag="PLG72_02515"
     CDS             11986..13059
                     /locus_tag="PLG72_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOJ79706.1"
                     /translation="MISPKIKDLNKWIAQIDYLSGSVCKLEYLKNVIKGIVIGIAMMV
                     PGVSGGTMAIVLGIYDRLVHSVATIFRDFKNNLLFLVQVGIGGAAGVLIFSRLMEGAM
                     TSYPYIMRYLIMGVIIGGLPLLFKKAAESRSGVRGEKACAREDGYYIKEDKAGRRESK
                     AAVRESTARPQKSRDTVTAGKADVLKNGPSAQSVWSRASDYIFLALGAAIVMLMSLEP
                     EAMIDMATDGSLTSYIFLVISGFIIAVGVVLPGISWSFMLLVLGMYEITLNAINTFNI
                     PFLIPLGIGIALGTFGTAKAVERFLQKYPRKTYMLIIGFVAGSLAEVYPGIPRGWQLP
                     ASAAAFIVGFAAIFILGKKGFAE"
     gene            13461..14831
                     /locus_tag="PLG72_02520"
     CDS             13461..14831
                     /locus_tag="PLG72_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOJ79707.1"
                     /translation="MDKLFGLKAQGTNARTEIVAGITTFMTMAYIIFVNPSILSQTGM
                     DWGAVFVATILAAAIATFIMAFVANVPYAQAPGMGLNAMFTYVVCLGMGLPWEQALAI
                     VFICGIINIVITVTKIRKMIIYAIPKSLQYAISGGIGLFIAYIGLIGGHILSFTPDGE
                     ANAAGAFSKIVPSIVDFTSPDSILTIIGLVIIVVLMLLKVKGAILIGIIATTLIGIPM
                     GVTQLPESFSFTVPSLAPTFLKLDIAGLFSDTSQLFRILALILTFSLADTFDTIGTFL
                     GTGRKTGIFDQLDEEALRNGKGFSSRLDRALFADATATSIGALLGTSNTTTYVESAAG
                     IAVGGKTGLTSATVGILFLLSLFLSPIAAMVPSAATSAALIIVGILMAESIGKIAWDE
                     LEEAIPAFFTVVVMPLAYSITTGIQVGFVFYCLTKIFTKKAKEVHPIMYIVTVIFLLD
                     YLIKAL"
     gene            14978..15514
                     /gene="purE"
                     /locus_tag="PLG72_02525"
     CDS             14978..15514
                     /gene="purE"
                     /locus_tag="PLG72_02525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOJ79708.1"
                     /translation="MATTADKGVKKGPKAALVMGSDSDYPVLKDCIKTLKDFGVETEV
                     LVCSAHRTPDKAARFASEAESSGIEVIIAAAGKAAHLPGVLAAYTPLPVIGVPVRSST
                     MDGLDSLLSIVQMPPGIPVATVAIDGAVNAALLAVQVMSGTHPELREKMRQYKAKLAR
                     KVEKKNEELQKKLREEGL"
     gene            15606..>15924
                     /locus_tag="PLG72_02530"
     CDS             15606..>15924
                     /locus_tag="PLG72_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOJ79709.1"
                     /translation="MIDYKAAGVDVEAGYEAVRLMRDDVRRTFRPEVLTDIGGFGGLF
                     ALDKAKYEEPVLVSGTDGVGTKLKIAFMMDRHDTVGIDAVAMCVNDIVCGGAEPLFFL
                     DYIA"
BASE COUNT         4382 a         3624 c         4347 g         3561 t
ORIGIN      
        1 gcctgtcctg ctcggcattc gatgggacat ccgagtcgag caggtagatg tttacggtgc
       61 ctacttttat ctgccagatc gaagcgtaga ctgtcctgcc gggcagttcc acgctgatat
      121 acagaggttc gccatttttg tcggtcacct ggctgacagg gagttcgtca atattgaggg
      181 tactgaaact tgtctcctgt atgccttcat ggtttatctt ctggttgaaa taaccctgcc
      241 tgtaaaaaag gcctactgcc gtgaacggga tacccagatc gctggcagat ttgcagtggt
      301 cgccggaaag cacgccaagc ccacccgagt atatggggat agtctcactg aggccgtact
      361 cggcggagaa atacgctacc atgtggccgt ccaggtccgg gtaggtcata ctgaaccaag
      421 tgtctgtgcg gttcatgtaa gcatcgaatt tgctgacgac attatcgtat atcttcagga
      481 agtcctggtc gccggccaat tgttccagcc tttgatttcc gatccttttt atcaaagcgg
      541 ccggatttcc gcccgtctct tcccacagcg acggatcagc catcctgaac atatcggcgg
      601 cttcgcagtt ccaggtccac caaagattgg ctgcgatgtc cctgagccgg aaaatccgtt
      661 gtggcatcac tgtctgtacg gttattctgc ctacaggttt cattcgatca ctccattgat
      721 tgtgtgaatt gcttaatata atgctgcata accagaatat tcactacagg ttttattaat
      781 gatagcacat ttttctcaat ctaaaattaa attttggtta agttttttta gccaatatat
      841 ttaaaatttt ccacggaaga aatggctata tctgcttaaa ctagcagtgt ataaaaatcg
      901 cctcggaaca ggcaggtcgg tattatgctc aagaggtttt tattttacgg attggcggga
      961 tggggcatcg agatggtctg gacgggcatg cactcgctgc tcggcgggga cctgaccctt
     1021 cccggacagt cgaatctctg gatgtttttc atctatggct gcgccgtttt cctcgaaccg
     1081 ctgcacgatg cgatctccgg atggaactgg atgctgcgtg gaatcgtctg ggtaatagtg
     1141 atatggatca tcgagtatgc cagcgggacg atactgtaca acgtgcttgg ggcatatccg
     1201 tggtactact cggacaggtt gtccgtgaac ggcctgataa cgcttgccta cggtccggca
     1261 tggttcgtgg cgggcctcat atttgagaag ttccacagga gccttgacag gtacggcata
     1321 gcctaagtat cgcagattgc gtttcagccc atattccatt aaaataatag ggcaaggcgg
     1381 aacggcaggt ccggctgaga atcggaattt ctgactgccc ggcatagaca gcctgacagg
     1441 cggctgaccg atacgatcgg gcggcttgca gcagatgccg gatatggggt tgagctgctt
     1501 ggacgttcgg caggagaagc cggatatggg attgagccgc ttggacgttc ggcaggagaa
     1561 gccggatata gggttgagtt gcttggacat tcagcagcag aagccggata tggggttgaa
     1621 ctgcttggac gttcggcagg agaagccgga tatgggattg agccgcttgg acgttcagca
     1681 gcagaagccc aatatagaat tgagttgctt aaccgttcgg caagagaaga aggtataagg
     1741 tttgcttata tgactgcccg gcagtataat ccggtatgca ttttatttga ttgttttccg
     1801 gcagaggatg aaaccgtctg ccggtgtgag aggggtcagg atggacaaaa ccgttgaaac
     1861 cggcaataaa acatttaaaa gcagatcata cccggcatac gcagacgaaa cgtaccgggg
     1921 atacgcggac atttcgcgcc tggaatatac aaccgtgaca cgcagcccaa atgcaggagg
     1981 aacaaggcat ggccacgcgg aaagaaccag gcgaaatcca ggcacggaag aaaggatccg
     2041 gcgcagcgca aaaccggcgc gcggatcatg gaaaaagcgt ctcttcgcgt ttttggtttt
     2101 ccagttcata tttgcgctgg tcacgatgcc tctgctcatc ttttacggcc cctttgagga
     2161 agtaaagggg accattgtcg gagcgtcgtg gaatacgctg agacaccagt acattgcgaa
     2221 gtttttcctc tcagatgaag caatagcgcg gatcctgagg gattcctacg ccgacgaccc
     2281 cacggcaaag ggtgaatcga tcgagaagat cgaactcagc ggtgaacatg acagcaccat
     2341 cgaagtttac aacatcgagg gcggcgattt caagggaaag ctcatggtgg tgcacgatcc
     2401 cacgaggata atagtcggct attcgtcgct gttccctgaa tcaggagaga ccacgagccg
     2461 gatagcgaag aacaacggcg gggtcgcagg catcaacgcc ggcgggttcc tcgacagcgg
     2521 atggacaggc accggcggca taccgatggg gtatatcatc cacgacggca aggtggtcta
     2581 cgaccagtat gagggcaagg atgtaaagca ggaaacagtg gcattcacaa atgaagggat
     2641 gctcatagta ggcaagcatg ccataaaaca gctgatggag tacggggtga aggaaggcgt
     2701 aagtttcggc cctcctctca tagtcaacgg caagccgact atcaaaaaag gcgacggagg
     2761 atggggcatc gctccccgta cggcgatcgg gcagagaaag accggcgaag tgctgctgct
     2821 tgtgattgac ggacgcacga tcgaatcatt gggagcgaca ctgagagaag tccaggacat
     2881 ccttctcgag tacggcgcgg taaatgccgc aaatcttgac ggcggttcat caacgacgat
     2941 gtattataac ggaaaggtaa tcaacaggcc ttcggataaa ctnnnnnnnn nncggcgaaa
     3001 ggctggtgcc ttcggtcttc gtggtgatgc cggagggcga acaggccggc atcggctaag
     3061 tcggaggtga caggagatga aaaacataaa aacgatttac aagtggatac ttatttctgt
     3121 catccttcag accgcattcc tttcctggct gaattacgtc taccttccca acagggggca
     3181 ctttaaatcc acgatgtatg aagcggagtt cctcgccgtc aaaaaccgga gctacagact
     3241 gcctgaggga gcggacgata tcagcgtgtc atatgacggc ctctatgccg cttacatgct
     3301 tgacgggcag atcgtgatcg cggatttgga cggcggcagg gaaataaaaa ggctcgggcc
     3361 tgccggcggg aaaatcacat accacaggtg gctcccggac agggaaatgc tcatatatgc
     3421 cataaaggag ccggaaggca ggagcgggcg tgtccggata tccacgcatg acgttatccc
     3481 cgacctggac aggagttacc cggacataaa gggcctgccc gagggcagtg ccgtcattga
     3541 tatagaactg tcccccatga ccaacatagt ttatccgaag atacagacca gcaaaacgag
     3601 agtcaggatc tacaggttcg atataatgga caacctgagc cttatgttca agacggacct
     3661 tacaacagtg gtaaaggaaa cgctgtactc cgacaacctg gtataccagg ttgccggtga
     3721 aaggacgcgc gtcaggaacg gcaaaaccgg gaaaacctca tatattcctg tgaaacaggc
     3781 ccacctgctg ctggatatag acggcaggga tgtcatatat gcgggtatta cggacaagtc
     3841 gggcagtgta tcggagatac gatacggaaa agcagggacg gaagcggctg aatggaacag
     3901 catcagcctc agtgatccgg cagcggcaga tgacataatg atcacggccg acgggtcagt
     3961 ttatatagct gaccggcagg cgaggactgt cgaatgcgtg aaaggcgtag aaggccggaa
     4021 gggccaaact gacggatcat ccgagaagca aagcggatcg gtcggacagg acggatcgac
     4081 cggtcagcgg aacggtttgt ccgattttaa gaatgggctc tctgactggc tggatggtat
     4141 gaaagagcag ttgaacggat caaaacggca gaacaggtca tcaggccgga acagcagatc
     4201 atccggccaa aataacatat ctaccggcca gcatgacgta tcatccggtc agaacggcaa
     4261 cgcatccgga caaaacgtcc cggcatccgc ccgacagggc gtgacggccg cacaggaccg
     4321 taagctgatc aggtacgaag gccggctcat cacgatgctt gaccagtaca tcgtttatct
     4381 cgacggtgaa aacctcgtgt tgagaacact ggaaaaatag atcagaatta ttccggacaa
     4441 tgctcatgcc gttgttgcct gttttattat gaaaattaag ggtaacaata atagaaatga
     4501 agcatttatt tgacagaagg ttaactgaaa tggcagaagg aaaagcgtta gctttgacaa
     4561 aagagaattt tgatgagata ctgaacggaa ctgataaact tgtggtcgtg gatttctggg
     4621 ctgcatggtg cggtccgtgc agggccgttg caccgataat cgaggaactg gcccgggatt
     4681 acgaaggcaa agccgtgata ggcaaggtga acgttgacga acagaatgag atatcataca
     4741 ggtaccggat catgagcatt ccgacgatca tgcttttcaa aggcggacag gtagttgata
     4801 aagtcgtcgg cgcgaggacc aaaagcgaac ttgcagaact gatcgataag aacctgtaat
     4861 ttttcggtct ggaacgtttc cagccatata ttccgcagac ccgttcctca aaagattatt
     4921 ttttattcag gcaggaattt gcgaatcggt attgaatata tagaaagaag attgctttga
     4981 gaagggggat tcctgtggct cgggaaaaac ggtttaggac ttctttcatg ggaggattca
     5041 agaaatccga tgtaaatgcg tatatcgaac ggatgcttaa agagttcgat gaaaaactaa
     5101 agagtaaaga cgacgaaatt tccgtactca ggaatcagaa cagggaactg aaatcgaagt
     5161 atgaagagtt gcagcaaaac gccgatgaaa tcaatgaagc cagggtaaag ataggcgatg
     5221 tgctgatcca ggccagggaa aaagccgaag ccatgctgga agacgcccgg gccgaagccg
     5281 cagaggagaa aaggcggctc gaggaagcca acgagcagga gagggagaag ctggtcgata
     5341 tccgaagcga tataaggaat atgagaacgg agatcgtgaa gctgctcaag aaatacgaag
     5401 aacagctttc ggaatatgac ggtcgtgtta cggacatcct gaaggacgac gcggaagaaa
     5461 cggccgatac tgaaacggcg gaggcggaag cggcaacagc ttcggatcat ggatccggtg
     5521 cggagaacgc gcccgggtat tcaaataata catggaacgg cggatctgca gattattagg
     5581 cgacttgtca gccggaagtc cggagttttt ccaaaaaaca cttgaaattg ccgcaggtaa
     5641 ttgctataat ctatgaaaat cacatataac ggcgatgatg gggaagagta gtcctgtgaa
     5701 tttcaaagag agccggaggt cggtgcgatc cggtaaagga gcaggatgaa ggcagccctg
     5761 gagcactggc ttgaaaaacc ggagtcttga gacagccggg caaaagtcag cgtggcctca
     5821 cgttacaggg gaagggcatt ggaatgtgcc cggaatgaga gaacgcttac agcgttaatt
     5881 agggtggtac cgcggattgc tccttcgtcc cttgcaaggg atgaaggagt tttttgttac
     5941 cacggcttcg gcaggctgat gccgccggag ctggccggat caaagccggt cgtatcgaag
     6001 ccggctgtgt tgaaccgata ttttgcatta ttaatttgta ataattttat atcaggcaaa
     6061 acgcgcagaa ggggtgttca ttatgagcag caagatggaa gaaatccttg agatcctcga
     6121 aaaaaatgca agggcgagcg ttgaagaaat tgccctcatg ctcgacatga gcgttgaaga
     6181 agtacaggcg gcgataagga agctggaaga agacaaggtc atagtcggct acaacacgct
     6241 tataaattgg gacaactcca acaaggaaac agttacggca ctcatcgagt taagggtcac
     6301 tccccagaga ggccagggct ttgacaagat cgcagagcgc atatacaggt acccgcaggt
     6361 caaggcatgc tacctcatgt ccggcagctt cgacctgact gtgatcatag aaggcaagaa
     6421 catgaaggaa gtggccttgt tcgtggccga gaagcttgct ccgctggagg gcgtggtcag
     6481 cacctctacc cactttgtat tgaaaaaata caagaacgac ggcgtgatat ttgaagaaaa
     6541 cggcaaagac gaaagagagg cgctgagact atgaaccttc aggaaatgat aaagccgacg
     6601 gtcaggaata tgccgccttc cggcatcagg aagtatttcg acctgctgaa tgaaatgaag
     6661 gacgccattt ccctgggcgt gggcgagcct gatttcgtca ccccctgggc gatcagggaa
     6721 gccggtatat attcccttga aaaaggccat acccattact cctccaatcc cgggttcatc
     6781 gagctaagga ggcagatttc acggtacgtg gcaaagaagt acaacgtgga atatgatcct
     6841 aaaacggaga tcatagtgac tgtcggcgcc agcgaagcga tagacattgc gctgagagcg
     6901 ctcggcggac ctggcgacga ggtcatcata cctgagccca gttttgtcgc atacaaggga
     6961 tgcaccatgt ttaccggggc gactccggtc gtcatagaac tgaaagaaga agacgagttc
     7021 aggctcaaac cggaacaact ggaagaggct ttgacggaaa ggacgaaggt tgtcatcctc
     7081 ccgtacccga acaaccccac aggcgccgtg atgcgggccg atgaccagag gaagatcgtc
     7141 gatgtgctta aggacaggga tgtgataatc atatccgacg agatatatgc cgaacttact
     7201 tacagcggaa agcacgtttc attcgcaagt tatcctgaaa tcaaggacaa aacgatcctg
     7261 atcaacggtt tctcaaaggc atttgcaatg accggatgga ggataggtta cgcctgtggg
     7321 ccccatgtac tcatcgaggc aatgaataaa atccaccagt atgccataat gtgttcgccg
     7381 acaatggccc agtacgccgc atctgaggct ctcaacagct gtgaggatga agtgcaggcg
     7441 atggtcaggg aatacaacag gagaaggcgc gtaatggtca agggattcag ggacgccgga
     7501 ctcagctgct tcgagccgat gggcgcgttc tatgtgttcc ccagcattaa atcgacgggc
     7561 atgacatccg acgaattctg cgagaaactc ctcatggagg agaaggtgct cacggtaccg
     7621 ggcacagctt tcggcgcctg tggcgagggc tatatcaggg ccacatacgc caactcgtac
     7681 gagaatatcg ccgaggcaat gaggcggatc aaaaggttcg taagcaggca caggaaatga
     7741 gaccgggccg catgttgtaa gcaggtacag gaaaaatgac agtgctgtgt gcgtgatgaa
     7801 cagatgcagg aaatttggca aggctgcaca cgatagctgg tacagaaagt gaaactagcc
     7861 tgaatgtggt aagcaggtaa agggaacgtg acaaagctat gtatgataag ccagtacacg
     7921 aagcgtgaca aggcggcttg taaaacactg gcaggcactg tcctcctgta ccactagctt
     7981 tatctttctg aactattact ctgtccttat ataccgctgt gctactgcac cgcttaacgc
     8041 tatccttctg taccattttt tcatttatgg gcgctggaaa ggtctgactt caaacttttc
     8101 tatgtttttt gtaccaaatt ttcgtttatg ggcgctcaag ttaagagtca agcttctttt
     8161 tacatcaata attgcaacaa agcgggcttt atgtaaacaa gaaagctata aaatggtaac
     8221 tttaacaaaa attacagccc ataaacgaaa aaacggtaca aattgcgctc aaaaacatac
     8281 attgcttcta acaggtaata aagtcctcaa gtgattatgc atctgttcaa acttcctggt
     8341 acgtatgtgt atacgtttgc cgaggcgggc ggcgagatta tgcgcaaaat ctctgcatgc
     8401 atttttatca tataattgaa tggtgttcat gtgtaagatt tcaatgcctt gtctcccgag
     8461 gatggctgaa cgtactgagt ccttgccctg ttctgatggc ctgttgtgat gagcaaaact
     8521 gtcatattcg acccctacct ttgcttgctt gaaatacagg tccacaaagc agcgatcttg
     8581 tttcagacgt atgctcatat cattgttcag cttaacttca tagttgaaaa tggcgcctct
     8641 catgccataa ccacccaata cgtatggcag cgtcaggatc atatatgcta gtgattccat
     8701 aatcgatgcg gaaccgtttt ctatatattt aagggctctt aacgcttttt tatgtccccg
     8761 atgtcccggg gtttttgaaa cgaatttttc aagcttttgc tttgtcgtaa cagccaatga
     8821 cgggttgccg ggcgggtgcg agcacaactg caaacctaac aggatcaggc gatgaatatc
     8881 caacctgttt gcaagttcca aaaacagcaa ctccggtgat gatactcttt tcccgttaag
     8941 tgtttttact gatttctccg gtagaaccag ttttgttgaa cggacttttt tgccgttaat
     9001 gcggaaacgc gcattataac tgttgactgt tatatcggag ctgtcacagc ccagggcatc
     9061 gtccccgata acagattcga tatatggtat attccacatc attgcagccg aaaagtgact
     9121 aatatgccct ttcatatgaa tatagtgcag catttttcgc cggatgtcaa ataatgggaa
     9181 atagcatcaa gttaaagttt cctctcactt atgcctttta cataatcggg ttcggaccag
     9241 gtttctgacc gacgctccgc tctttcggaa tatcgctcca ctcttcatac caacgattca
     9301 ctctttcgta ctagcactcc gctctcagaa cgtattcccg ataacatcga aaaaggcaga
     9361 gccactggat ataacgtatc tctgaaagag ttgcgtcaca gtttatccct gcgctgtgaa
     9421 gggccctgga caactgcttt ttcgtgaaca ggtgacggag cccgtacttt aagccggcag
     9481 tgacaaaccg ctcgtaagct ttctgctgat tctcagggat atccggcagg ctttttttct
     9541 tcagatggaa cagcaccacg tcgttacatg ccaaaaaagt gagaactgtc cccataaaaa
     9601 acttttacaa ccgcccgttt tctgttatag tatataggtg cattgtcaca atctatataa
     9661 aaatataata gaagagaaaa aacaaaggag ttgatcagga tgcttgaact ggaacagttc
     9721 aaacaggaaa tcgagagcct gggcaaagat ataaaggaaa tgggggcttc tctttgacat
     9781 cgccaggctg aaactcgaaa tcgaggaact ggaaaacaag gctgcagaac cggatttctg
     9841 gaatgatatc gagaactccc agaaggtcct gcagagaacg aagactttga agacaaaagt
     9901 cgaaacctat gaaaaactgc tttcggatct cgaggatctc aaagtcctca atgaactggg
     9961 catcgaagag caggacgaat cggtcatacc tgaagtgaag caggaactgg agagacttaa
    10021 ggactcatac agcaagctca aactcgaaac actgctcagc ggcccatatg acaggaacaa
    10081 cgccattatg acgctgcatg cgggggcggg cggcacggag gcgcaggact gggtgcagat
    10141 gctgctcagg atgtataccc gatgggccga agaccaccag tatgaggtca aaacgctgga
    10201 ttacctggac ggggatgaag ccgggatcaa aagcgtgacg atacagataa tcggcgaaaa
    10261 tgcatacgga tacctccgtt ccgagaaggg ggtacaccgt ctcgtcagga tatcgccgtt
    10321 cgacgcatcg ggcaggaggc acacatcttt cgcatcggtg gatgtgatgc cggaactgga
    10381 tgaggaaata gaggttgaga taaaccctga agacctcaag atagatacct acagatccag
    10441 cggcgcgggc ggccagcacg tcaacaagac cgaatcggcc gtaaggatca cacacatccc
    10501 gacaggcatc gttgtggcat gccagaatga gcggtcgcag atacagaaca gggagaccgc
    10561 gatgaaaatg ctcaaggcaa agctgctgga actgaaggaa cgtgaacaga aagagaagat
    10621 cgaagacctc aagggcgtcc agatggacat cgcctggggc agccagataa ggtcgtatgt
    10681 attctgtcca tacacgatgg tgaaggacca caggaccaac tacgagacag gcgacgtgca
    10741 ggcagtgatg gacggagatc tggataaatt catacatgca tatctttcaa tgaagcagta
    10801 atggggatgc cggctcggcg tccccgcttc cgaagaggta tcggagcatg gtttacaata
    10861 agttttcgga ttaccttaaa aaaagatatg gggcaaaagt atacaaactg cccgtcagcc
    10921 tgagtacgag ctgccccaac agggacggca ctgtcaggat ggaaacggac gccgtgatca
    10981 ggacacagac ggatgacggc ggcagccgcg cggaaaatta cgacgacacc gtagactgcg
    11041 ctgttgacga aggatcatac tgcggctgca ttttctgcgg catcgaggga tcaggcttcg
    11101 agacgctgcc ggcggaaaac agcatcaggg aacagcttga aaccaacatg cgctatattg
    11161 gcgaaaaata cagcgctgaa aaatttatcg catatttcca gaactattca aacacctacc
    11221 tccctttcga cagattctca aaagccatga acgaagcgtg catcgaaggg atagtcgccc
    11281 tttacatcgg gaccaggccc gactgcatcg acgacaggcg cgtggacttc ctgaagcagt
    11341 taaaggaaga gaaaggcgtc gacatcgtta tcgagcttgg gctgcagtct gttaatcacc
    11401 atacccttcg gtggctcaac aggggccacg gcctggccga gttcatcgac gccgtgctcc
    11461 ggatcaaaag agcaggcctt gaggtctgcg tccatatgat aaacgacctg cctgcagatg
    11521 atatctgcga cgtcatcgag gcggcaaaag tgctttctgc ccttggaacg gaccaggtca
    11581 agtgccactc gctgtacata cttgaaaaca ccgtgctggg ccgggcttac aaggaaggca
    11641 ggttcgcgcc gctaagcatg gacgagttca tcgaaaggac catcagcttt cttgaatacc
    11701 tggacccgga tatcgtggtc cagcggctta tcggaaggtc gccttccggc aggtcgctat
    11761 tctgcaactg gtataccagc tggtggaaga tccatgatat gatagtcgaa aagatggtaa
    11821 aagaagggcg gtaccagggg aagtattttg attatctcaa cggccatgcg ctgaagcatt
    11881 tcaaagacgt gtaaagtaca aaagccctca aaaacgtata acctgtgaaa aaagacatgc
    11941 ctgcaataag catataactt atggagaaag caaggcgtgt gaaaaatgat aagcccgaaa
    12001 ataaaagacc ttaacaaatg gatcgctcag attgactact taagcgggag tgtgtgcaaa
    12061 ttggagtatc ttaagaatgt tatcaaaggt atcgtgatcg ggatcgccat gatggtgccg
    12121 ggcgtaagcg gcggaacaat ggcgatcgtg cttgggatat atgacaggct ggtccacagc
    12181 gttgcgacta ttttcaggga ttttaagaac aacctgctgt tccttgtaca ggtggggatt
    12241 ggaggagcag ccggagtact catcttcagc cgcctgatgg aaggcgcgat gaccagctat
    12301 ccgtatataa tgcggtatct tatcatgggc gtcatcatag gcggactgcc gctgctcttc
    12361 aaaaaggcgg cggagagtcg ttccggtgtg cgcggggaaa aagcctgcgc ccgggaagac
    12421 ggatactata taaaggaaga caaagcaggc aggcgggaga gcaaagctgc cgtccgggag
    12481 agcacggcac gcccgcaaaa aagcagagat actgtgaccg caggcaaagc agatgtgctg
    12541 aaaaacggac cctccgcgca gagtgtatgg agccgtgcct ccgattacat attccttgcg
    12601 cttggtgcag ccatagtcat gctgatgtcg cttgagcccg aggctatgat agacatggcg
    12661 accgatggga gcctgactag ttatattttc ctggtgatct cgggattcat tatcgctgtc
    12721 ggagtcgtgc tcccgggcat aagctggtcg ttcatgcttt tggtgctcgg gatgtacgag
    12781 atcacgctca acgctataaa tactttcaac atcccgttcc ttatcccgct gggcattggc
    12841 atagccctcg ggacattcgg cactgcgaaa gcagttgaga gattcctgca gaaatatccg
    12901 agaaaaacat atatgctcat aatcggtttt gttgcaggtt ctctggcgga agtatacccc
    12961 ggcatccccc ggggctggca gctgccggca tccgccgcgg cattcatcgt tggtttcgca
    13021 gcgatcttca tcctcggcaa aaagggattt gcggaataat cgcgtaaaag ctttgccggg
    13081 atatgcatat aaggggattc gcgagcattt aaatgaatat gcgcgattat acgaataata
    13141 caaaagagac catcgaaaat gttcgtgttt tgtgttttta ttggttgaca aaatgagttc
    13201 tcatttgata gtattagaaa cgtgaaagga atattggaat aacgacgtga aatgcttgaa
    13261 atataagggt ccggaggact ttgaccatat gaacgttttt tagcgggcag ttacagtttt
    13321 acgtaagcat aatgtaccaa cccagtataa ctcaggaatg aaagtacgaa cattggcgtc
    13381 ttgcggtaca ggcttgaccg ggcgtgccga acgtccgtta agtaattatc tcttaaatct
    13441 catgggaggg gaattttatg ttggacaagc tgttcggact caaggctcag ggtacgaatg
    13501 ccaggactga gatcgtagca ggcataacca cattcatgac gatggcttac atcatcttcg
    13561 tgaacccgag catactcagc cagactggca tggactgggg tgccgtattc gtagcgacga
    13621 tcctggcggc tgccatcgca acgttcatca tggctttcgt ggctaacgta ccgtatgcgc
    13681 aggcaccagg aatggggctc aacgccatgt tcacctacgt ggtgtgtctt ggaatgggac
    13741 tgccctggga acaggcattg gcaatcgttt tcatctgcgg tattatcaac atcgtcatta
    13801 cagtcacaaa aatcaggaaa atgatcatct atgcgatccc gaaatccctg cagtacgcaa
    13861 tcagcggcgg tatcggactg ttcatcgcat acatcggtct gatcggcggc cacatcctca
    13921 gctttacgcc tgacggagaa gcaaatgcag ccggcgcttt cagcaaaata gtaccttcga
    13981 tagtggactt tacaagccct gattcgatcc tgacgatcat cggactggta ataatcgtag
    14041 tattgatgct cctcaaggtc aaaggtgcga tcctcatcgg aattatcgca actacgctga
    14101 taggcatacc gatgggagta acacagcttc cggaatcttt ctcattcaca gtgccgagcc
    14161 ttgcgccgac attcttaaag cttgatattg caggactgtt cagtgatact tctcagttat
    14221 tcaggatact tgcactcata ctgacattca gccttgccga tacgttcgat accataggga
    14281 cattcctcgg caccggcagg aagacaggca tattcgacca gttggacgaa gaagcgctcc
    14341 gcaatggcaa aggattctct tccaggcttg acagggcgct gttcgctgac gcaactgcaa
    14401 catccatcgg tgctctgctc ggtaccagca acactaccac gtatgttgaa agtgcagccg
    14461 gtatagcagt aggcggaaag accggtctga catctgcaac ggtcggaata ctgttcctgc
    14521 tgtcgctctt cctctcgcct atcgcagcaa tggttccttc ggcagctacc tcggcagcac
    14581 ttataatcgt cggtatactg atggcagaat ccattggaaa gatcgcatgg gacgaactgg
    14641 aagaagcaat acccgcattc ttcacagtag tcgtcatgcc tttggcttac agcatcacga
    14701 caggtattca ggtaggattc gtattctact gcttaaccaa gatcttcacc aagaaggcaa
    14761 aagaagtgca tcccattatg tacatcgtta cggtgatctt cctgctggat tacctgatca
    14821 aggcgctgta atcgtgacaa taaaatgcat ttcaatgatg aagtccgtca tatcaggcgg
    14881 acttctttag cataaggaag atttttgtat tacaatatat gtatatatcg tttgtgcgat
    14941 aagatttaca tatgcataac cggggaggaa gcaatttatg gcgacaactg cggataaagg
    15001 cgtgaaaaaa gggcctaaag ctgcactggt gatgggcagc gattccgact atcctgtact
    15061 gaaggattgc atcaaaacat tgaaggactt cggagtggaa acagaggtgc tggtctgctc
    15121 ggcacaccgc acgccggaca aggccgcccg ctttgcttct gaagctgagt ccagcggcat
    15181 agaagtgata atagcagcag caggtaaagc tgcgcacctt ccgggagtcc tcgcggcata
    15241 tacgccgctt ccggtcatag gggtaccggt caggtcatcg acgatggacg gactcgactc
    15301 attgctttcc atcgtgcaga tgcctccggg gatacccgtt gcgactgtcg caatagacgg
    15361 agctgtaaac gcggcgctgc tggcggtcca ggtgatgtca ggaacacatc ccgaactgcg
    15421 tgagaagatg cggcaataca aggctaagtt ggcaaggaaa gtggaaaaga aaaacgaaga
    15481 actgcagaag aagctccggg aagaagggtt ataggcagaa gtgtcgctgc actttctgca
    15541 gtaagtacag aatattgagc aatgtttgat gcccaaaatg aaaattcatt ggaggatgat
    15601 tttgcatgat cgactacaaa gcggccggcg ttgatgtaga agccggttac gaagccgtaa
    15661 ggctgatgag ggacgatgta aggcgtacat tcaggcccga ggtgcttacc gatataggcg
    15721 gcttcggagg actgttcgca ctggacaagg ccaagtacga ggaaccggtg ctggtgtcgg
    15781 gtacggatgg cgtcggtaca aagctgaaga tcgcgttcat gatggacagg cacgacacgg
    15841 tgggcatcga cgctgtggca atgtgcgtca acgatatcgt atgcggagga gccgagccgc
    15901 tgtttttcct cgattacata gcct
//
LOCUS       DAOSCC010000024        15603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2591_length_15603_cov_4.562645, whole genome shotgun sequence.
ACCESSION   DAOSCC010000024 DAOSCC010000000
VERSION     DAOSCC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15603
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2591_length_15603_cov_4.562645"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLG72_02535"
     CDS             <1..1086
                     /locus_tag="PLG72_02535"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOJ79710.1"
                     /translation="GPAKGGIRFHPNETVDTIRALSMWMTWKCAVADIPLGGGKGGII
                     CDPRTMSEREQEQLCRGYVRQLARNMGPNLDVPAPDVMTNARHMLWMLDEYEAINGGR
                     YPGTITGKPVGMGGSLGRTEATGFGVIYVLREALKKLNMKPESTTASIQGFGNVAQYA
                     AKMYSQLGGKIVAISCWDNTDKKAYTYRKADGINCDELVSITDSFGSINKEKAKQLGY
                     EILDGGAWIGQDVDIIIPAALENQITAENVNRISRQVKIIVEAANGPTTTEADKVIND
                     RGIFLVPDFLANAGGVTCSYFEQVQCNMNYFWDKDEVLTKLDRVMTSAFHAVYDLSVS
                     RKLNMRDAAYVIAIERVAKAVKSRGWT"
     gene            1181..1624
                     /locus_tag="PLG72_02540"
     CDS             1181..1624
                     /locus_tag="PLG72_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79711.1"
                     /translation="MIDKRKAAGIILAGALVFTGTVSLAANEPAQQTPPCKAENVPAS
                     PVTREQVKERINNALDKLVDDKVITQEQKDAVLKAMEARRPCKVKERDGKPGDKGGKP
                     GDKRLRCSKHGVLQDLVKDGTITQEQADAIREAIRSVYKSIKKPE"
     gene            complement(1739..2878)
                     /locus_tag="PLG72_02545"
     CDS             complement(1739..2878)
                     /locus_tag="PLG72_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HOJ79712.1"
                     /translation="MKGAFYTGKYRNVFSEYGYDEEEIRNRVEDTWNKLFYGDENTRI
                     YWPVGDDMGYILDTGNNDVRTEGMSYGMMMCVQMDKKEEFDRIWKWTYTYMLHRSGKY
                     KGYFAWSLNSDGTRRAEGPAPDGEEYFAMALLFASKRWGDGPEPFDYSRQARNILREV
                     VHKGEDGVGDPMWEPNLKLIKFIPESPYTDPSYHLPHFYELFSIMGNPEDSDFWKEAA
                     EASRRFLKTACHPVTGLAPEYSEFDGRPRKAPWGYGHHHFYSDSYRVAANIGLDYEWF
                     GKDEWQRTEADRIQAFFAPMGANADYRMYAIDGTPLEEKALHPVGLLATNAMASLAAN
                     DTENRRKCVELLWNTPVRTGVRRYYDNCLYFFAMLALSGNYRIWL"
     gene            complement(2958..3422)
                     /locus_tag="PLG72_02550"
     CDS             complement(2958..3422)
                     /locus_tag="PLG72_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOJ79713.1"
                     /translation="MNVEYINPFIEASQTVFRQIASMDAKLGKVYLKTSSYRSEDIII
                     MVGLTGKIRGQANFSMKKEFGLKLASSMMMGMPVTELDDMSKSAIAELANMIMGNAAT
                     LLYNRGIQIDITPPSLLTGENIHISQSRMRTICIPLLVGDDTVELDISTEEL"
     gene            complement(3627..3983)
                     /gene="spoVAE"
                     /locus_tag="PLG72_02555"
     CDS             complement(3627..3983)
                     /gene="spoVAE"
                     /locus_tag="PLG72_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOJ79714.1"
                     /translation="MQYINAFVIGGLICAAGQVLIDKTKLTPARILVLFVSLGVVLTG
                     LGIYQSIVEVGGAGATIPLTGFGYSLARGAFNEVDKYGLLGAFTGGIRATAAGVAAAV
                     FFSYLAAVAVKPKAKT"
     gene            complement(3998..5023)
                     /gene="spoVAD"
                     /locus_tag="PLG72_02560"
     CDS             complement(3998..5023)
                     /gene="spoVAD"
                     /locus_tag="PLG72_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOJ79715.1"
                     /translation="MFEKKTGKQTFQMQNPPSIYATASIVGPKEGRGPLADYFDQAVD
                     DEFWGEKSWEKAESKFVREVLAKVVAKASMPMDALDFVIAGDLLNQCIAANFGIRESN
                     VPFYGIYGACSTIGEAMQLGAMLIDGGFASNVACLTSSHFCSAEKQFRFPLELGSQRP
                     PTAQWTVTGSGAVILTAEGDGPYITRITTGKIVDMGIKDQNNMGAAMAPAAADTIYAH
                     FEDTGLPHDYYDMIVTGDLGVVGKRILDDLLSSNGIRIGGILNDCGLMIYDQKQDPHA
                     GGSGCGCAATVFAGFLYDRLKKGKLNRILFVPTGALLSPTSVQQGETIPCIAHAVAVE
                     NTEGGDQ"
     gene            complement(5045..5491)
                     /gene="spoVAC"
                     /locus_tag="PLG72_02565"
     CDS             complement(5045..5491)
                     /gene="spoVAC"
                     /locus_tag="PLG72_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOJ79716.1"
                     /translation="MQRVMTKDEYRKYVDKVSPDSKTARNVLRAFMVGGFICVIGQFF
                     LNSFKNMGLDQDTASSLTSIVMILIGVSLTALNLYDDIGRFAGAGSIVPITGFANSIA
                     SPAIEYKSEGYVLGVGARMFLVAGPVLVYGISMSIIFGIIHYLLRS"
     gene            complement(5511..5663)
                     /locus_tag="PLG72_02570"
     CDS             complement(5511..5663)
                     /locus_tag="PLG72_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79717.1"
                     /translation="MLIILNRYKVLFAILFIILIAAVIWLTLSARSSKIPSNGVFVIR
                     ELSLPI"
     gene            complement(5791..6555)
                     /gene="sigF"
                     /locus_tag="PLG72_02575"
     CDS             complement(5791..6555)
                     /gene="sigF"
                     /locus_tag="PLG72_02575"
                     /inference="COORDINATES: protein motif:HMM:NF004052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HOJ79718.1"
                     /translation="MAEDTYNDREAYKDKDTTELILLAKGGDRLAQSMLVENNIGLVW
                     SIVRRFQNRGHETEDLFQIGCIGLIKAINKFDTSYDVRFSTYAVPMIIGEIKRFLRDD
                     GIIKVSRSLKEVSNKVRIVKEILVKELGREPTISELAQRLDIPMDEIVMAIEAGHSPE
                     SLFSPINEGDSSSLMLIDKLNDECSDDEMDIIDRIALREVMDTLKPREKQIIILRYFK
                     EKTQVQIARMLGISQVQVSRIEKKILEDIRNRISVR"
     gene            complement(6738..7172)
                     /gene="spoIIAB"
                     /locus_tag="PLG72_02580"
     CDS             complement(6738..7172)
                     /gene="spoIIAB"
                     /locus_tag="PLG72_02580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOJ79719.1"
                     /translation="MQPVNEMKLEFLSKSSNEAFARVVAAAFVSQLDPTIEELADVRT
                     AVSEAVTNAIIHGYENEIGIITMVCRLYDRSVEIEITDKGKGIEDIELAMQPLYTSKP
                     EMERSGMGFTVMESFMDRVKVRSKPGEGTTVTLFKTFSSEKK"
     gene            complement(7238..7582)
                     /locus_tag="PLG72_02585"
     CDS             complement(7238..7582)
                     /locus_tag="PLG72_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512129.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOJ79720.1"
                     /translation="MEIRFSNRGSTLIAAFSGELDHHFAEYARSKIERELFKATTRDV
                     IFDLTGLSFMDSSGIGVLVGRYANISKLGGKAAIICRNSRIRKILEISGVLKLMPVYD
                     ELDDAMRALHSA"
     gene            complement(7707..8696)
                     /gene="whiA"
                     /locus_tag="PLG72_02590"
     CDS             complement(7707..8696)
                     /gene="whiA"
                     /locus_tag="PLG72_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOJ79721.1"
                     /translation="MSFSSSVKGELCRLENMDECCVLSELAAVVWLNGAAAPVPGDGF
                     NIRITTENAAFARWIYSSIRKYCGIVPEVTIRKSRKLKKHVTYMLFITSVSGSDILLK
                     KTGMSAVAYGDRCELALARKIEQQQTCCVKSFLRGAFLAGGSISDPEKTYHLELTSRT
                     AEQADEIRRLMALYGLNGRTIIRKGSYVTYLKEGENIVDFLNVIGAHRALMELENVRI
                     LKGMRNNVNRIVNCETANLGKTVNASVRQVENILYIVENIGLDKLPPGLREIAELRLE
                     HKDLSLTELGQMLNPPLGKSGVNHRLRKLDRIAEKHRGGPPEHKEEEAGEEDS"
     gene            complement(8793..10826)
                     /locus_tag="PLG72_02595"
     CDS             complement(8793..10826)
                     /locus_tag="PLG72_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOJ79722.1"
                     /translation="MRFGKSEWSSFDRGIEREWLLTNGIGGFSSSTVIGASSRRYHGL
                     LVASLEPPVERHLVLSQLHETVTLNGQEFSLSSFSTGNYINTGYLFQASFELDPLPVF
                     TYAIADVLIEKTVSLVYGENTAVVCYRIITGNDDAEIKVAPLVNFRDYHGDSSKHHMK
                     FNQTYGNDHTVIRPYDLDLRILVGCAGSSYEALDDCWFEGMYYPVESERGLNAFEDHY
                     IPGVFRITAKAGSDTCFYFICTVCQDGPDVLASRAWNAGGYMDPQYGKSAVEAEIKRI
                     GSLTAGCSDDFHRALVRSADHFIVHRRSTNSKTIIAGYPWFTDWGRDTMISLPGLTLP
                     TGRYEDAADILAAFSRYVRHGLVPNMFPDRGGEPVYNTVDAALWYFEAICKYVEETGD
                     TALVKDILYDSMLQIIDSYIDGTLYGIHMDEDMLIAAGSGDTQLTWMDAKAGDTVFTP
                     RHGKAVEINALWYNALRVMENISGSFGYDAGRYGRIADRVKESFTRVFWYEEGKYLYD
                     VVGGTRDKRVRPNQILAVSLSYPVIDGEMARQVVAKVWKELYTAYGLRTLSRDSEDYR
                     GRYLGDMYQRDSAYHQGTVWPWLIGHFITAFARTLGRDPVYADMPAKFIRPFIDHMRH
                     ACIESISEIFDGDEPHMPRGCFAQAWSVAEVLRAYVGHVLKRDGHGQDADAQA"
     gene            complement(10888..11970)
                     /locus_tag="PLG72_02600"
     CDS             complement(10888..11970)
                     /locus_tag="PLG72_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOJ79723.1"
                     /translation="MMLGETTGSIFGKRARQGDGLRGDGRSDPRQEQLMKGPKVVAIG
                     GGTGLSTMLRGLKAFTSNITAVVTVADDGGGSGVLRQELGMLPPGDIRNCILALADTE
                     PLMEQLLQYRFKEGNLKGQSFGNLFLAAMDGISSSFVEAVKRMSNVLAVTGRVLPVTL
                     ENVTLCAELEDGSIVCGESRIGQHRNFNKCRIKRVYMEPADAEPLEDVIDALDQADVI
                     VLGPGSLYTSILPNLLVRGICQAIQRSNALKIYVCNVMTQPSETEDYSVSDHIRALED
                     HSCKGIIDLCIVNNSVIPDDIREKYQNDGADQVVIDRETVENAGIRIIEGDYASLRNN
                     LVRHDPHKLAEAVIKAAIEDMAIKRR"
     gene            complement(11967..12842)
                     /gene="rapZ"
                     /locus_tag="PLG72_02605"
     CDS             complement(11967..12842)
                     /gene="rapZ"
                     /locus_tag="PLG72_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOJ79724.1"
                     /translation="MRFIVITGISGAGKSLVANYLEDAGFYCIDNLPPLLIPKIAEIC
                     SQSYRKMDKIALVIDIRGGELLNDLFPALDALKKDGYTYEILFMEASDNVVIKRYKES
                     RRSHPLDHGGSLKKAIAEERRVLEKIKDHANYVIDTSNLTPKQMKEAITGIIVNSEEF
                     GGLIINIISFGFKYGIPIECDLVFDVRFIPNPYYIESMKKHTGKHEDVRNYVLGMPET
                     REFNDKLNALLDFLIPNYVKEGKSQLVIGIGCTGGRHRSVVIADELMKTLTEKKHRVV
                     IEHRDIDKDGRSGGR"
     gene            complement(12886..13800)
                     /gene="murB"
                     /locus_tag="PLG72_02610"
     CDS             complement(12886..13800)
                     /gene="murB"
                     /locus_tag="PLG72_02610"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041719580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOJ79725.1"
                     /translation="MDKFKAAELIRQIPGSGTVLIDEPMSRHTSFRIGGPADIFIRPG
                     SIESAVGIINACRDNCIPAVVIGNGTNLIVRDGGIRAAVIQLAGNVSGYEVTGEAITA
                     EAGILISRLSRIALEHGLSGLEFAEGIPGTLGGAVTMNAGAYDGEMSKVVSRTEYLRP
                     DGSICWLDNEQHRFGKRSSIIQADGGTVLRTVVRLEKGDKKGIKEKMDDFNARRHEKQ
                     PLDMPSAGSIFKRPEGYYAGKLVQDCGLRGYRIGGAEVSALHCGFIVNTGNATARDVI
                     SLIEYIRDSVYKKFGVVLETEVKIIGED"
     gene            complement(13986..14738)
                     /locus_tag="PLG72_02615"
     CDS             complement(13986..14738)
                     /locus_tag="PLG72_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOJ79726.1"
                     /translation="MKLVADTHTHTISSGHAYSTIAENAAQAKINGMEAIAMTDHGPA
                     LRGASTEVHFMNLGVIPEYIDGIRIFRGAETDIMDFSGTLDIPDKILSRLEFVIASYH
                     DIVIRPGSVEENTEGMINALKNPFVDAVAHPGNPVFQVDIDRVVKAALEYGKLIEINN
                     GSFRVRKGSYDNCFEFVKKCKKYGVSVTCGTDAHICYDIGKFGKVLEILVEGQMPEEL
                     VLCTSPEKVEDFVKRRKAAKKSAAAELSGKQA"
     gene            complement(14868..>15603)
                     /gene="hprK"
                     /locus_tag="PLG72_02620"
     CDS             complement(14868..>15603)
                     /gene="hprK"
                     /locus_tag="PLG72_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOJ79727.1"
                     /translation="TYLASLSPQERKKRLDDYFRTGFPCLVIARDQDPFPEMMEASEK
                     YSIPILRTKDITSRFMSGLIRYLNVMLAPRISMHGVLVEVYGEGILILGESGVGKSET
                     ALELVKRGHRLIADDLVEIRRVSDTTLLGAAPENIRHLIEIRGIGIVDVKHLFGMGAV
                     KMQENINLVIKLELWKEGKMYDRLGLEDHFTDILGISVPSLVIPVRPGRNLAIIVEVA
                     AMNHRQKKMGYNAAKFLSERFFKFDD"
BASE COUNT         3433 a         4430 c         3586 g         4154 t
ORIGIN      
        1 ggccctgcca aaggcggtat caggttccat cccaatgaga ccgttgatac gatccgcgcc
       61 ctctccatgt ggatgacatg gaaatgcgcg gttgcagata tcccgcttgg cggcggcaaa
      121 ggcggaataa tttgcgatcc caggaccatg tcggaacgtg aacaggaaca actgtgccgc
      181 ggctatgtac gccaactggc gcgcaatatg ggccccaacc tggacgtgcc tgctcctgac
      241 gtcatgacga atgccaggca catgctctgg atgctggacg aatatgaagc aatcaacggc
      301 ggcaggtatc ccggaacgat cacgggcaaa cccgtaggca tgggaggatc cctcggaagg
      361 actgaagcca caggcttcgg cgttatctat gtcctccgcg aggcgcttaa aaaactgaat
      421 atgaagcctg aaagcacaac ggccagcatc cagggattcg gcaacgtcgc ccagtatgca
      481 gctaaaatgt acagccagct tggcggtaag atagtcgcca tctcctgctg ggacaacacg
      541 gataagaaag catacactta ccgcaaggca gacggtatca actgtgacga gctcgtttcc
      601 atcaccgatt cattcggatc cataaacaag gaaaaggcaa agcaacttgg ctatgagatc
      661 ctggatggcg gagcatggat cgggcaggac gttgatatca tcatcccggc agccctggag
      721 aaccagatca cagccgagaa tgtgaacagg ataagcaggc aggttaagat aatagtcgag
      781 gctgccaacg ggccgacgac caccgaagca gacaaggtca tcaacgaccg cggcatattc
      841 cttgtacctg atttcctggc aaatgccgga ggagtcacct gcagttattt cgaacaggta
      901 cagtgcaaca tgaattactt ctgggacaag gacgaagtcc tgacaaagct cgacagggtc
      961 atgacttcag ctttccatgc agtatacgac ctgtccgtaa gcaggaaact caacatgcgc
     1021 gacgcagctt atgtcatcgc tatagagcgt gtggccaaag cagtgaaaag ccgcggctgg
     1081 acctgacata aatttccttt cgtcatgtgc atcataaata cacgctcctg tacaaaaaat
     1141 aagcacagtc tgacttgatc aattttaagg agtgatgcac atgatcgaca aaaggaaagc
     1201 cgccggcatc atactggcag gagccctggt tttcaccggg actgtctcgc ttgccgccaa
     1261 tgagccggcg cagcagacgc ctccctgtaa ggcggagaat gtaccggcat ccccggtgac
     1321 ccgggaacag gtaaaggaac gcatcaataa tgctcttgac aaacttgtcg atgacaaagt
     1381 catcacacag gagcagaaag acgcggtgct gaaggcaatg gaagcaaggc ggccgtgcaa
     1441 agtcaaagaa agagacggga aaccgggaga taaaggcgga aaaccagggg acaagcgctt
     1501 aagatgcagc aaacacggag tgcttcagga cctggtaaag gacgggacca ttacgcagga
     1561 gcaggctgac gcgatcaggg aagcgatcag gtctgtatac aaaagcataa aaaaaccgga
     1621 gtgattccgg tgaaaaagat ccgggccctc aaacgggtcc ggatcttttt agtatagtta
     1681 tcaatgctgc gttcaaattc tataaatgat atagaccatc atacagttga taatatcatc
     1741 acagccatat cctgtagttg ccgctgagag ccagcatggc aaagaaatac aggcagttgt
     1801 cgtaatagcg cctgactcca gtgcggacag gagtattcca cagcagttcg acgcatttcc
     1861 gcctgttctc cgtgtcgttc gcagcaagcg aagccatcgc gttggtcgct aacagcccga
     1921 caggatgcaa tgctttttcc tccagcggcg taccgtcgat ggcatacatg cggtaatcgg
     1981 cattcgcacc catcggggca aaaaatgcct gtatcctgtc ggcctctgta cgctgccatt
     2041 catcctttcc gaaccattcg taatcaagcc ctatattggc ggctacacgg taagaatcgc
     2101 tgtaaaagtg gtggtggcca tatccccatg gagctttacg gggccttccg tcaaactccg
     2161 aatactcggg cgcaagccct gtcacaggat ggcatgcggt tttgaggaag cgtctgctgg
     2221 cttcggcagc ctctttccag aaatcgctgt cctccggatt tcccattatg gaaaacaact
     2281 catagaaatg cggcaggtga tacgacggat ctgtataggg gctttcagga atgaacttga
     2341 tcagtttaag gttcggttcc cacatcggat caccgacacc gtcttctccc ttatgtacga
     2401 cttctctcaa tatattcctg gcctgcctgc tgtaatcgaa cggctccggt ccgtcgcccc
     2461 agcgcttcga agcaaaaagc agagccatgg cgaaatactc ctcgccgtcc ggcgcgggcc
     2521 cttctgcacg ccttgtaccg tccgaattca gcgaccacgc aaaataccct ttatatttgc
     2581 cggaacggtg gagcatatat gtatatgtcc atttccagat ccggtcgaac tcttccttct
     2641 tgtccatctg cacgcacatc atcatgccgt aggacatgcc ttccgtgcgc acgtcgttat
     2701 tgccggtatc cagtatgtat cccatgtcat cgccgacggg ccagtaaatc ctggtgttct
     2761 catccccgta gaacagcttg ttccatgtgt cttcgaccct gtttcggatc tcttcctcat
     2821 cgtacccgta ttcactgaaa acattcctgt acttacccgt ataaaacgcg cctttcatcc
     2881 gctcatcctc ccatcagcaa aagataaccc atataaaagc ctgcgccacg atatgggcac
     2941 gattggaata ctgcacacta taattcttcc gtagatatat ccagttctac cgtatcgtct
     3001 ccgaccagca ggggaatgca gatggtcctc atcctgctct gagatatatg gatattttcg
     3061 cctgtcagca gcgacggcgg cgttatgtcg atctgtatgc ccctgttgta cagcagcgtc
     3121 gccgcatttc ccatgatcat gtttgccagt tctgcgatgg cgcttttaga catgtcatca
     3181 agttcggtca caggcatgcc catcatcatg ctggaagcca gcttcagccc gaactccttt
     3241 ttcatcgaga agttcgcctg tccgcggatc tttcctgtca ggcccaccat tattattatg
     3301 tcttcgcttc tgtaggagga ggttttaaga taaacctttc ctagttttgc atccatcgat
     3361 gctatctgtc tgaaaactgt ctgacttgct tcgataaatg gattgatata ctcgacattc
     3421 atttaaacac ctcacattta agcatatttc tttcggatgt actatttagt attctatatc
     3481 cgagaataaa atccttcctt tccggacttt tttcgctctt ttttgacatt tatttgtatc
     3541 cgctgcagca taaatcaagt tatgtttata tataacacca atacagataa caataacacc
     3601 ggatcgtatt aaatttttca attttatcag gttttcgcct tgggtttgac tgcaacagcc
     3661 gcaagatatg aaaagaaaac ggctgcagcg acaccggcgg cagtagccct gataccgccc
     3721 gtgaatgcgc ctaacaggcc gtatttatcg acttcgttga acgctcccct ggcaaggctg
     3781 tacccaaagc ctgtcagcgg gatagtagca ccggcgccgc cgacttccac tatgctctga
     3841 tatatcccca agccggtcag caccaccccg agcgatacga acaggaccag tatccttgcc
     3901 ggcgtcagct tggtcttgtc gatgagcacc tggcctgctg cgcagataag tccccctatt
     3961 acgaaagcgt ttatatactg catgatcgtt tcccgcatca ctgatctcct ccttcggtat
     4021 tttccactgc aaccgcatgg gctatacacg ggatcgtctc tccctgctgg acgcttgtcg
     4081 gactgagcaa tgctcccgtg ggcacgaaaa ggatcctgtt cagtttccct tttttcagcc
     4141 tgtcataaag gaaaccggca aaaaccgtcg cggcacagcc gcagccgctt ccgcccgcat
     4201 gcgggtcctg cttctggtca tagatcataa ggccgcagtc gttcaggatg ccgcctatgc
     4261 gtatgccgtt cgaagaaagc agatcgtcaa gtatgcgttt gccgaccacg cccaggtcgc
     4321 cggtcacgat catgtcataa tagtcatgag gcaggcccgt gtcctcgaaa tgcgcatata
     4381 tcgtgtcggc ggctgccggc gccatcgccg cgcccatgtt gttctggtcc tttatgccca
     4441 tatccacgat ctttccggtc gtgatcctgg ttatatacgg accgtcccct tccgccgtca
     4501 gaataacggc gcccgagccc gtcactgtcc actgagccgt cggcggcctc tgggatccca
     4561 attccagcgg gaacctgaac tgcttctccg cagaacagaa atggctcgac gtcaggcatg
     4621 ccacgttgga ggcaaaacct ccgtcaatca gcatcgctcc aagctgcata gcctctccta
     4681 tagtcgaaca tgcgccgtat atcccgtaaa atggcacgtt ggattcacgt atgccgaaat
     4741 ttgcggcgat acactggttc aaaaggtcgc cggcaatgac aaagtcaagc gcatccatcg
     4801 gcatggacgc ttttgcgacg acctttgcaa gcacttccct cacgaacttg ctttctgcct
     4861 tctcccagct tttttctccc cagaattcat catccaccgc ctggtcgaaa taatctgcga
     4921 gaggccctct tccctctttc ggccctacaa tggatgccgt tgcatatatc gatggagggt
     4981 tctgcatctg aaacgtctgt ttaccggttt ttttctcaaa catgcacgtt tctccctttc
     5041 aacgtcagga tcttagcaga taatggatta ttccaaagat gatggacatg gatatcccgt
     5101 ataccaggac aggcccggct accaggaaca tccttgcgcc tactccgagc acataacctt
     5161 cactcttgta ttctatagcc ggagacgcta ttgaatttgc aaatcctgtg atcggcacta
     5221 tggacccggc tcctgcgaat ctgccgatgt catcgtacag gttcagcgcc gtcagtgaga
     5281 ctccgatcag tatcatgact atcgatgtga gcgaagacgc ggtatcctgg tcgagtccca
     5341 tgtttttgaa ggaattaagg aaaaactggc cgattacaca aataaaaccg ccgaccataa
     5401 atgccctgag gacgttcctt gccgtttttg aatcagggga caccttgtcg acgtatttcc
     5461 tgtattcgtc tttcgtcata acccgctgca caggctgtct cctccttcgg tcatattggt
     5521 aacgagagct ccctgatgac gaaaaccccg ttcgagggga tcttgctgct cctggccgag
     5581 agcgtcagcc agatcaccgc tgcgatgagg attatgaaaa ggatcgcaaa aagcaccttg
     5641 tacctgttca gaatgataag catggtatac actccgattt atccgatttt aattttatta
     5701 ttggaacatt ggagaaaagt atgcgcggaa attttgcgcc ggctgcaaaa tgtgccggca
     5761 agccgcgcat ttatatttga tatggctgcg tcacctgacg cttatgcggt ttcggatgtc
     5821 ttccagtatc tttttttcga tccttgagac ctggacctgt gaaatgccca gcatccttgc
     5881 tatctggacc tgtgtctttt ccttgaaata cctgagtatt atgatttgct tttctctcgg
     5941 cttcagcgta tccataactt ctctcagcgc tatcctgtcg atgatatcca tctcgtcgtc
     6001 actgcattcg tcattgagct tgtcgatgag catcagcgat gaactgtccc cttcgtttat
     6061 cgggctgaac agcgactccg gcgaatgccc ggcttcgata gccattacga tctcatccat
     6121 cggtatatcc aggcgctggg ccagttcgct tatggtaggc tccctaccaa gttccttgac
     6181 cagtatttcc tttactatcc ggaccttgtt ggatacttcc ttcagcgacc tgctgacctt
     6241 tatgatgccg tcatccctga ggaatctctt tatctcgcct atgatcatgg gaacggcata
     6301 tgtggagaac cttacatcgt atgatgtgtc aaatttgttt atcgctttaa tgagcccgat
     6361 gcaccctatc tggaacagat cctctgtctc gtgccccctg ttctggaacc tgcgcacgat
     6421 gctccacaca aggcctatat tgttctcgac aagcatgctc tgggcaagcc tgtctccccc
     6481 cttggccaac agtatgagct ctgtcgtgtc cttgtcctta tatgcttctc tgtcgttata
     6541 tgtatcctca gccatatatc cgaactatcc cccgcctgat catcattcgt acagggcatt
     6601 atgtctacta tatgcattta ataacattat tgccaataat ttactgcttc atgcaaaaaa
     6661 aataaaattt ccgttttcat caagcagaga gcacgcttct tcatgatgaa agcctgctcc
     6721 ttctccgaaa agcatcttta cttcttctcc gaactgaatg tcttgaaaag ggtcacagtg
     6781 gtcccctctc ccggtttcga cctgactttt accctgtcca taaagctttc catgacggtg
     6841 aatcccatcc cggaacgttc catttccggt tttgaagtat acagcggctg catggcaagt
     6901 tcgatgtcct ctatgccctt gcccttgtcc gtaatttcta tctcgacgct tctgtcatac
     6961 agccggcaaa ccatcgtgat gatgccgatc tcgttctcat agccatgtat tatcgcgttc
     7021 gtaacggctt cagacactgc tgtccgtaca tccgccagtt cctctatggt aggatcgagc
     7081 tgcgaaacga aggcggccgc caccaccctc gcgaaagctt cattgctcga tttgctcaga
     7141 aattcaagtt tcatttcatt aacaggctgc atatgatttt ctccccttca gattcactta
     7201 agcaaagaac atacggccat aacgaagaac atggacgtca cgcgctatgg agcgctctca
     7261 tggcatcatc cagttcatca tacaccggca tgagcttcaa tacccctgat atttcaagta
     7321 tcttcctgat cctgctgttc ctgcagatga tcgccgcctt tcccccgagc ttgctgatgt
     7381 tcgcatacct gcccaccagg acgccgatgc cggaactgtc catgaaactg agacccgtga
     7441 gatcgaatat cacgtctctc gtcgtggcct tgaacagttc cctttcgatt ttgcttcggg
     7501 catattcagc aaagtggtgg tcaagttcgc cggaaaatgc tgcgatcagg gtggaccccc
     7561 tgttagaaaa ccttatttcc aatactatcc ctccgaaaat tcctttgcgt tactgtatta
     7621 ttctttaaac tccaggaatt tccttcatag aattatggaa taatttgtaa cttggataag
     7681 ttttttattt caaataacga caaacttcag ctatcttctt cgccggcctc ttcttcctta
     7741 tgctcaggcg gtcctcccct gtgtttttcc gcgatcctgt cgagtttcct cagcctgtgg
     7801 ttcacaccgg acttaccaag cggagggttc agcatctgtc ccagttccgt caggctgagg
     7861 tccttgtgtt ccagcctgag ttccgcgatt tcgcgcaagc ctggcggcag cttgtccagg
     7921 ccgatatttt caacgatata gaggatgttt tccacctgcc tgactgaagc gttcacagtc
     7981 tttccaaggt tggccgtctc acagttcact atcctgttca cattgttgcg catgcccttg
     8041 aggatgcgta cattctccag ttccatcaac gccctgtggg ctccgatcac gttaaggaaa
     8101 tccactatgt tttccccttc cttgaggtat gtcacatagc ttccctttcg tatgatcgtc
     8161 cttccgttca gcccgtataa ggccatcagg cgtctgatct catcggcctg ttcggcggtc
     8221 ctggacgtaa gctccaggtg atatgtcttt tccggatcgc ttatggaacc tccggcaagg
     8281 aatgcaccac gcaggaacga cttgacgcag catgtctgct gctgttcgat tttccgggca
     8341 agcgccagtt cacaccggtc gccgtacgcc acggcagaca tgcccgtctt tttcagcagt
     8401 atatctgaac cggacaccga tgttatgaac agcatatatg tgacatgctt cttaagcttc
     8461 ctgcttttgc ggatcgtcac ttcaggaacg ataccgcagt atttccttat gctgctgtat
     8521 atccagcggg caaatgccgc attttccgtg gttatcctta tattgaagcc gtctcccggc
     8581 accggcgctg ctgcgccgtt gagccatacc accgcggcaa gctccgacag cacacagcat
     8641 tcgtccatgt tttccagcct gcacagttcg cccttgaccg acgacgaaaa tgacaattgc
     8701 atcacctctg tttttatctt atccgatagt ccggtccatg ttgatgtcag ctaagtctat
     8761 acttatgctc atatttgaat caatgccggc attcatgcct gcgcatcagc atcctgcccg
     8821 tgtccgtcac gcttcagcac atgtccgaca taagcgcgga gcacttccgc cacgctccag
     8881 gcctgcgcga aacagcctct cggcatatgc ggctcatcgc cgtcgaatat ctcggaaatg
     8941 ctctcaatgc aggcatggcg catgtgatcg atgaagggcc ttatgaattt cgcgggcata
     9001 tcggcataaa ccgggtccct gccaagcgtc cttgcaaacg ccgtgatgaa atggccgatc
     9061 agccacggcc acacagtacc ctggtgatat gccgagtccc tctggtacat atcgcccagg
     9121 taacgccccc tgtaatcttc cgagtcccgc gaaagagtcc tgaggccgta tgcggtatat
     9181 agctccttcc agaccttcgc caccacctgc cgtgccattt ctccgtcaat tacgggatat
     9241 gacaggctca cggccaggat ctggttgggc cttaccctct tgtccctggt gccgccgaca
     9301 acatcgtaca ggtatttgcc ttcttcatac cagaacaccc tggtgaagct ttccttcaca
     9361 cggtctgcga tccgtccgta cctgccggcg tcatatccga acgaccctga tatgttttcc
     9421 attactctca gcgcattgta ccacagcgcg tttatctcca cagcttttcc atgccttggc
     9481 gtgaacacag tatcgcccgc ctttgcgtcc atccacgtca gttgggtatc cccgcttccg
     9541 gcagctatga gcatgtcttc gtccatgtgt atgccataga gcgttccgtc gatataactg
     9601 tcgattatct gcagcatcga atcatataat atgtctttta ccagggcagt gtcgcctgtt
     9661 tcttccacgt atttgcaaat cgcttcaaaa taccacaggg cggcgtcgac cgtattgtag
     9721 acgggctcgc ctcccctgtc ggggaacatg ttcggtacaa ggccgtgcct tacatacctg
     9781 ctgaatgctg caaggatatc ggctgcatct tcgtacctgc ccgttggcaa tgtcagcccc
     9841 ggcagagaga tcatcgtgtc ccttccccag tcggtgaacc atggatatcc ggcgattatg
     9901 gtctttgagt tggttgaacg cctgtgtacg ataaagtggt ccgcgcttct gaccagtgcc
     9961 cggtggaaat cgtcgctgca gcctgcggtg agggacccta tcctttttat ctcggcctcg
    10021 acggcagact tcccatactg cggatccatg tatccgcctg cattccaggc acggcttgca
    10081 agaacgtcgg gcccgtcctg gcaaacggtg cagatgaagt aaaaacatgt gtcgctgccc
    10141 gcttttgctg ttatcctgaa aacaccgggt atatagtggt cttcgaaggc atttagcccc
    10201 ctttcactct ccaccgggta atacatgccc tcgaaccagc agtcatccag ggcttcgtag
    10261 ctgctgccgg cacatccgac cagtatcctg aggtccaggt cataaggccg gatgaccgtg
    10321 tggtcattcc cgtacgtctg gttgaacttc atgtgatgtt ttgaggaatc gccgtgataa
    10381 tccctgaaat ttacgagcgg tgcaactttg atctctgcat cgtcgtttcc ggttatgatc
    10441 cggtagcaga ctaccgcagt attttcacca tagacaagcg agactgtttt ctcgatgaga
    10501 acgtccgcga tagcatatgt gaagacaggc agcggatcaa gttcgaagct tgcctggaaa
    10561 aggtatcccg tattgatata gttccctgtg gaaaaggacg aaaggctgaa ttcctggccg
    10621 ttgagagtca cggtttcatg cagttgggac aggaccaggt gcctttcaac aggaggttcg
    10681 agtgatgcaa ccaacagccc gtgatatctg cggctgctgg ctccgatgac cgtagaagag
    10741 gaaaatccgc ctatgccgtt cgtaaggagc cattcccgct cgatgcccct gtcaaagctg
    10801 ctccattccg atttaccgaa cctcatccgc ccgccccctc ctctcgtttc agtccgccat
    10861 aagcactatc agcatgccgg aatcagttca tcgtctttta atggccatgt cttcaatggc
    10921 ggccttgatc acggcttccg caagcttgtg cggatcatgc cttacaaggt tgttcctgag
    10981 cgacgcataa tcgccttcta tgatccttat gcctgcgttc tccaccgtct cccggtcgat
    11041 gaccacctgg tcggctccgt cgttctgata tttttccctt atgtcatcag gaataaccga
    11101 gttgttgacg atgcacaggt cgatgatgcc cttgcacgaa tggtcctcca gggccctgat
    11161 atggtcggaa acactgtaat cttccgtttc cgacggctgg gtcatcacat tgcatacgta
    11221 tattttaagc gcgttgcttc tttgtattgc ctgacagatc cccctgacca gcaggttagg
    11281 aaggatgctc gtataaaggc tgcccggtcc gagcactata acatctgcct ggtctaacgc
    11341 atcaattacg tcttcaagcg gctcggcgtc cgcaggctcc atgtacaccc tctttatcct
    11401 gcatttgttg aaattcctgt gctgccctat gcgcgactcg ccgcagacta tgctgccgtc
    11461 ctccagttcg gcgcacagcg tcacattttc cagtgtgaca ggaaggaccc tgcctgtaac
    11521 cgcaaggacg ttgctcatcc ttttgaccgc ttccacgaag ctggaagata tgccgtccat
    11581 tgccgcaagg aacaggttcc cgaaactctg ccctttcagg ttcccttcct tgaacctgta
    11641 ctgcaggagc tgttccatca gcggttctgt atccgcaagc gcaagtatgc agttccgtat
    11701 gtcaccagga ggcagcatgc ccaattcctg cctgagcacg cccgaaccgc ctccgtcatc
    11761 ggctacggtg acgacagcgg tgatattcga tgtgaatgcc ttcaatccgc gcagcatcgt
    11821 cgaaaggcct gtgcctcccc cgatcgccac gaccttggga cctttcatga gttgttcctg
    11881 ccggggatcg cttcttccgt ctccccgcaa accatcaccc tgacgggctc ttttgccgaa
    11941 tatgcttccg gtggtttccc cgagcatcat ctgccaccac tcctgccatc cttatcgata
    12001 tccctgtgtt ctatgactac cctgtgtttt ttctccgtca gggttttcat aagctcatcg
    12061 gcaatgacta ccgaacggtg acgtccgccg gtacagccta tgccgatcac cagttgtgac
    12121 ttgccttcct ttacgtagtt gggtatcagg aaatccagca atgcgttcag tttatcgttg
    12181 aattctctgg tctcgggcat cccaagcacg taattccgga catcttcatg tttgcctgtg
    12241 tgttttttca tggattctat ataatacgga ttaggtatga acctgacatc aaacaccagg
    12301 tcgcattcta tcggtatgcc atatttgaac ccgaaagaaa ttatatttat gatcaggccg
    12361 ccgaattcct cgctgttgac tatgatgcct gtgatggctt ccttcatctg cttcggcgtc
    12421 aggttggagg tgtctatgac ataattcgca tggtccttta ttttttcaag gacccgcctc
    12481 tcttccgcga tcgccttttt caggctgcct ccatggtcaa ggggatgtga acgcctggat
    12541 tctttataac gctttatgac aacgttgtcg gaagcctcca tgaaaaggat ctcgtaggta
    12601 taaccgtctt ttttaagggc gtcaagcgca ggaaacaggt cattcaggag ctctccgccg
    12661 cgtatgtcga tgaccagcgc tatcttgtcc atcttcctgt agctctgcga acatatctcg
    12721 gctatcttgg gtatcagcag cggcgggagg ttgtctatgc agtagaatcc cgcgtcttcg
    12781 agataattgg ccacaaggct ctttcctgcc cccgatatcc cggttattac gataaatctc
    12841 atacttgatc tctccgtttc ttacctccgg tttttgcttt cgctttcagt cctcgccgat
    12901 tatcttcact tccgtctcga gaactactcc gaacttctta tatacggaat ccctgatata
    12961 ttctatcaga gatatgacat ctcgtgccgt cgcgtttccg gtattgacga taaagccgca
    13021 gtgcagcgcc gatacttccg ccccgcctat cctgtaccct ctgaggccgc agtcctggac
    13081 aagcttcccg gcataatagc cttccggcct tttaaaaatg cttcccgcgc tgggcatatc
    13141 cagcggctgt ttctcgtgcc tcctggcatt gaagtcgtcc atcttttcct ttattccttt
    13201 tttatcgccc ttttccagcc tgacgacagt ccgcagcacc gttccgccgt ccgcctggat
    13261 gatgctcgag cgtttcccga acctgtgctg ctcgttgtca agccagcaga tgcttccgtc
    13321 cggccggagg tactcggtac gactgaccac cttcgacatc tctccgtcat atgcccctgc
    13381 attcatggtg acagcacctc caagggtccc cgggatgcct tcggcgaatt ccagcccgga
    13441 aagcccgtgt tccagcgcta tccgggacaa cctggatatg agtatgcctg cctcggcggt
    13501 tatagcttca ccggtcacct catatccgct gacattgccg gccaactgga taactgcagc
    13561 ccggatgcct ccgtccctta ctatcaggtt tgtgccgttc ccgatgacta ctgccggtat
    13621 gcagttatcc ctgcaggcgt ttattattcc aacggcgctt tcgatgctcc cgggccttat
    13681 gaatatatcg gcaggcccgc cgatccgaaa cgatgtatgc cgggacatgg gctcatcgat
    13741 caggacggtc ccgctgccag gtatctgtct tatcagttct gctgctttaa atttatccaa
    13801 accggattct ccctcgataa tattctctta aaatatattt tcttaagtca tattttaccg
    13861 gtacattcta tccaaaaaac cggatccagc ctttttactt ttgtttcatt tatcttttta
    13921 taatacagcc ctgtttattt ctctcattta cgtcttatcc cgttattacg gctgcctcaa
    13981 tccggctatg cctgtttccc gctcaattcg gcggctgccg atttcttggc agctttcctt
    14041 cgtttgacga agtcctcgac cttttccggg gatgtacaca gcacgagttc ttcgggcatc
    14101 tgtccttcga caagtatctc aagcaccttt ccgaatttcc ctatatcata acatatatga
    14161 gcgtcggtgc cgcaagtgac actcacgcca tattttttgc atttctttac gaactcgaag
    14221 cagttatcgt aagacccttt cctcaccctg aaagagccgt tgttgatctc tatcagcttt
    14281 ccgtactcca gcgcggcttt cacgaccctg tcgatgtcga cctggaatac tgggttgccc
    14341 ggatgggcaa ctgcgtcgac aaagggattt ttaagcgcat ttatcatgcc ttccgtattc
    14401 tcttcgacac tgccgggcct gatcacgatg tcatggtagc tcgctatcac aaactccagc
    14461 ctcgaaagta ttttatccgg tatgtcaagg gtacctgaaa aatccattat atcagtctcg
    14521 gctcccctga atatccttat gccgtctatg tattcaggta tcacgccaag attcatgaaa
    14581 tgcacttcag tggaagcccc tctcagggca ggcccgtgat cggtcatcgc tatggcttcc
    14641 ataccgttta tcttcgcctg ggcggcgttt tcagcaatcg tgctgtacgc gtgcccgctt
    14701 gaaatcgtgt gtgtatgtgt atctgcgacc agtttcaaaa cgtaactcct cctgttttcc
    14761 agtttcatgt gtgtactaat gcacggatca gtatttcccc gccgctgtcg tgctgcagga
    14821 taccaggccc ggctgctgcg gtttaagtca tcggatttgc cgagccttca gtcgtcgaac
    14881 ttgaagaagc gctcgctcag aaacttagcc gcattatagc ccatcttctt ctgcctgtgg
    14941 ttcatcgccg caacttccac gatgatggcg aggttcctgc ccgggcgcac cggtatgacc
    15001 agggacggca cggaaatgcc gaggatatcc gtaaagtggt cctccaggcc caggcggtcg
    15061 tacatcttgc cttctttcca gagttccagt tttatcacaa ggtttatatt ctcctgcatc
    15121 ttgaccgcgc ccataccgaa caggtgcttt acatcgacta tccctatgcc gcgtatttct
    15181 atcaggtgcc ttatattctc aggcgctgcg cccagcagcg tagtatccga tactctcctg
    15241 atctccacca ggtcatctgc gatgagcctg tgccctcttt ttacaagctc cagggccgtc
    15301 tcgctcttac cgacgccgct ttctccaagg atcaaaatgc cctcgccata tacctccacg
    15361 agcactccgt gcatggatat cctcggagcc agcatcacat tgagataacg gatgaggccg
    15421 ctcatgaacc tggatgttat atcctttgtc ctcaggatag gtatcgagta tttctcagat
    15481 gcctccatca tttccgggaa cggatcctgg tccctcgcga tgacgaggca ggggaatccc
    15541 gtgcggaaat aatcgtccag cctctttttc cgctcctggg gcgacaggct tgcaaggtat
    15601 gtc
//
LOCUS       DAOSCC010000025        15431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2621_length_15431_cov_4.637812, whole genome shotgun sequence.
ACCESSION   DAOSCC010000025 DAOSCC010000000
VERSION     DAOSCC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15431
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_15431_cov_4.637812"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLG72_02625"
     CDS             <1..736
                     /locus_tag="PLG72_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ79728.1"
                     /translation="SCTSPLRAAYGWQRDGGMAPYILADEKDLVPLPDELTYTDGAQI
                     ACGFGTVYEGLEKIGISGNDAVLVVGLGPVGLATLMLAKAMGAQKLIGVDVVKERCEI
                     ALDKKLADHVFISGPDTLEKVLALTGGHGVERAVDSSGHTSGRQLAIRATRKWGRIAF
                     VGEGGTCEFNPSPDIIHDQKTIYGSWVTSIWKMEELTEKLVRWGIHPEDLVTHRFTLD
                     NVAEAYRLMAEGKCGKVAVVFDEEIK"
     gene            complement(886..2307)
                     /locus_tag="PLG72_02630"
     CDS             complement(886..2307)
                     /locus_tag="PLG72_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ79729.1"
                     /translation="MTEKKQGFNPYLPSWEYIPDAEPYVFDGRVYIYGSHDRFNGYVY
                     CLNDYVCWSAPVDDLTDWRYEGVIYRKTDDPLNPDGSMCLYAPDVTVGPDGRYYLYYV
                     LDKVPVVSVAVCDKPAGKYEFYGYVHYPDGTRLGEREGDQPQFDPGVLTEGDRTYLYT
                     GFCPRGDKSRKGPMATVLGPDMLTIVEEPVFIAPSEPYSKGSGFEGHEFFEAPSIRKR
                     GDTYYFIFSSVVFHELCYATSKYPTKGFTYRGVIVSNSDLGIDTYKPADKPMFYGGNN
                     HGSIVEINGQWYIFYHRHTNGTNFSRQACLEPITFREDGSIIQAEMTSCGPNGGPLAG
                     RGEYPTYIACNLFCSEESVYTDWTASWMNNQFPKITQDGKDGDEEIGYIANMKDSATA
                     GFKYFDCRGVKRIKIKTRGYARGVFEVRTKWDGPVLGTIPIEFSNIWKEYSADIAIPD
                     GVHALYFTYKGHGSAHFGSFTLE"
     gene            2764..3576
                     /locus_tag="PLG72_02635"
     CDS             2764..3576
                     /locus_tag="PLG72_02635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HOJ79730.1"
                     /translation="MQINLLVTLDSKYIRPLKVMLASMFINNPEENFHVYLVHSSIKD
                     EEIADLGQFMDGRGQKLSVIKIDDGYFQDAPLVFHYTKEMYYRLLAYKFLPEELDRIL
                     YLDPDILVLNPVRGFYETDLDGYLYAAAFHNVIAVKGLNQIRLYPYKINAYINSGVLL
                     MNLELQRKLIDEEAIYTFIEKNRAKLFMPDQDIINALYADKIKCLDEKIYNYDARFYN
                     YYKMVTSNKCDMDFVIRNTVFIHFCGKKKPWRKGYSGKFHSLYKHYEKLAIS"
     gene            complement(3670..4539)
                     /locus_tag="PLG72_02640"
     CDS             complement(3670..4539)
                     /locus_tag="PLG72_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOJ79731.1"
                     /translation="MKPDDFYSTFEKDFTSWAVATDDIRAAFVVGSRARADHPADQWA
                     DLDIILYADNSDFYLHNIDWLKSLGNIWVTFAYQTSGGEPERLTLFEGGFQVDIVFLA
                     SDSLYRLVKDRSIPYNFRRGVRVLVDKDNTSRYILPPDFKPAASPAIDERSFVQVVSM
                     FFFGALYTAKQILRNELWAAKARENDLRKLMLQMMEWHAKALNGNDYDVWHAGRFLHE
                     WIDQKVLQELKTTFSLYDRADSLRGLLASIDLFRRISVETADKLHLKYPADTDHHITN
                     WIRENTRDLANGE"
     gene            complement(4976..5515)
                     /locus_tag="PLG72_02645"
     CDS             complement(4976..5515)
                     /locus_tag="PLG72_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ79732.1"
                     /translation="MVSRSRLALNNQLRLLWEQHVVWTRLTIISMVFDLPDVDPVTGR
                     LLRNPKDFEALLKPLYGDGTAARFADLLTSHLVIAAELVKASKAGDTGAAADAEKRWY
                     ANADEIAVFLGSINPCWSARQWQAMLREHLALTKSEAVNILTGKYAEGITVFDQIEKQ
                     ALEMADVMVNGIIRQFPNI"
     gene            complement(5667..7223)
                     /locus_tag="PLG72_02650"
     CDS             complement(5667..7223)
                     /locus_tag="PLG72_02650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79733.1"
                     /translation="MTKDRPKGKLLKLSNTGCGVLDMFDPEQVRRFYNDYGEKEWDRL
                     DKSAYQRLVYHNHVKFMEPHIGPGKRVLDAGCGPGRFSIHIAQSGSKVTLLDISESQL
                     TLARQKINERGLDVQVERYIRADVTNLDMISDSYFDTVICYGAVLNYLFSNDRKAVDE
                     LKRVTKNNGVLLLSVNSLYGVLRSCAVNEGMVPENFWGRPEYWKIHEVARTGDLIHPD
                     VAHPSRHLYTAAELKKLLEDAGLADIELGASPSIMSGLRPQAELLEKDETAWKTILDI
                     EDLSYCVGHLADAGEHLLAKGKVRKGSKPETEDAGSAAARNGAPDAVDSGDGVRNPDI
                     TIETDRLILREFREEDWKEIHHYSSDEDVIRFMPWGPNTEVDTRNFVDMALSEKEKIP
                     RKEYHLVVTEKRTGSLIGAVELSLEGYDNRQGMIGYCYSKKAWGQGYGTEAAAALIKF
                     GFEKLNLHRIRANCDTFNIASQRILEKNGMRREACFRKSAFIRGEWRDSYQYAILKEE
                     WEAKAKGKKP"
     gene            complement(7598..7827)
                     /locus_tag="PLG72_02655"
                     /pseudo
     CDS             complement(7598..7827)
                     /locus_tag="PLG72_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361551.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
     gene            complement(7958..8806)
                     /locus_tag="PLG72_02660"
     CDS             complement(7958..8806)
                     /locus_tag="PLG72_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOJ79734.1"
                     /translation="MRSEQEMMDLILSVAQADERIRAVLMSGSRANPAVPKDKYQDYD
                     ITYFVEDITPFRNDPAWAEERFGKPLIMQMPEAMRYPEGDGTFAYMMIFPDGVRIDIT
                     FDHRKYIDNGEPAVVLLDKDNGRGFLPPLPAPSDEHWHIEPPSPLFYYSCCNNFWWCL
                     NNVAKGIARDELPYVMHMLNEVVRHELHDMINYYIGAKRGFSLSTGKSGKYFKKYLPQ
                     ELYAQYAATYSGSDYADIWAAIDTMCDLFHTLAVEVAAHFGFTYRQEEEDGIREYLRM
                     VREQVL"
     gene            complement(8872..9360)
                     /locus_tag="PLG72_02665"
     CDS             complement(8872..9360)
                     /locus_tag="PLG72_02665"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ79735.1"
                     /translation="MSMQNGGIKGKVLKALAEKKMCYIATAGENNVDNAMVAYYSDGF
                     DLYFGSFSDTLKCRNIGVNPNVAVCIDNIQIHGRAQQLEYGSSEYLKYKEKYLAKFPG
                     YKFYFELENNELYKITPLVIWYYDSSKGTMHRDVIIFDDGYYKEIAPYEPPGEFKKRR
                     EV"
     gene            9786..11762
                     /locus_tag="PLG72_02670"
     CDS             9786..11762
                     /locus_tag="PLG72_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ79736.1"
                     /translation="MKYYVDANAPKDGDGSVNRPFKRISEAAKVALPGDEVLVAPGIY
                     REYVDPVHSGTEDARITYVSTTPLGAVITGAEQVKTWKHYQGNVWVCRIPNSVFGDYN
                     PYTTLVYGDWYFATPNKHTGCVYLNDKAMYEAVTLEECIKGEVYECSWVPEDSIYKWY
                     TEQDQDADETVIYANFQGLDPNKENVEINVRRRCFMPSKTGVGYITVRGFKINKAATT
                     WAPPAAFQDGMIGPHWSKGWIIEDCEISHSKCAGISLGKYLDPDNEQYFTHKHVKSPT
                     QMERDAVCRGQYHGWLKEKVGSHIVRRNNIHHCEQGGIIGRMGAVFSIIEDNHIHHIN
                     NMSELGGAEIAGIKIHAAIDVIMRRNHIHHCTMGIWCDWEAQGTRITQNLLHDNQRPP
                     YAKFLPGSMMCQDIFVEVGHGPTLIDNNVLLSDISLRIATEGVAMVHNLICGAFTSVG
                     SGTHPRYTPYHIPHRTEVMGFMTILHGDDRFYNNIFIQKWPADKKDGDEKEGQDNRQV
                     GTHVFDEYPTYDEWIAQFDFTKRPDMMGLAPAHSGHLPVWCEGNVYLGGAKPWKKETK
                     FLAVDNCGQEPKVELVEKDGEYYLDTNIYDLIGDFSGRMINTEVLGKAFEPEQYFENP
                     DGTPIRFDADYFGNHRGIRVIPGPFAAPAGHIKL"
     gene            12150..13055
                     /locus_tag="PLG72_02675"
     CDS             12150..13055
                     /locus_tag="PLG72_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79737.1"
                     /translation="MLYQETFQSGENPSEISVSSYIYINGKLEDNMITVGFPDHCHSY
                     FEIQYFFSGSGETRIDGKPIRTVPNSLYLIPPLHVHGQSGHERMVNLVIQFSHTFLSS
                     NAFAWKKNSTLLPAGEMKKSGSIMVEPGSRLSRYIAEIEEVSPRFETWLPESEKKFEY
                     TVEYEWKLHSATLGIITELLDTGNLVISENSGDVSETIKLHAVLNRLVTHPEEKLDMK
                     KAAKLACMSYSNFSRAFKEAIGRSYVDYCNSLRVHRAEELLKTTKISITNISQMLGFG
                     SVSYFNRIFRAYTGSTPMRYRKESM"
     gene            13290..14657
                     /locus_tag="PLG72_02680"
     CDS             13290..14657
                     /locus_tag="PLG72_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ79738.1"
                     /translation="MKKLLAILLAVVMIVMVGACGTKNNGGTTTPNSPGGSNEEPGTP
                     APGGESDTLTGELDIWAWGADDEATARKDAIEVFIKNHPNLKVTYTVIPTADQVWDQK
                     SSAALASGSAGDVMQMSPDWYGMNTKYYIDLNPLVQRDGIDLDAVITPGLIDRYYDAD
                     GKLEGLPLHTNCFVIAYNKDMFAEAGVPEPKEGWTLQDLADWGKAFAKGSGANQTYAM
                     AKHWCMNNFMIYSMGGIPYTEDLSTPKMGTEEIVKALGLYKELLDNGIIPTDTAQKTI
                     PAQTLFTSGKAAMYTMGGFEAAQVIRDAEENGINLGFAPMPSDPNGKEINVQYATGWA
                     ITTTAKNVDAAWAFLKESSFANDEMGTINARVGIPANRKIAESVYAQTKIGNVEFTNN
                     YYVQHISNAHMNPFGGTLASSGNIWTTMVEEVTLNGQDPAAVVAKYAPQIEAEFASYE
                     FNAKK"
     gene            14754..>15431
                     /locus_tag="PLG72_02685"
     CDS             14754..>15431
                     /locus_tag="PLG72_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ79739.1"
                     /translation="MKLKERSLSLKRREALNFYLYISPWLLSFLVLTIYPMLDSFLLS
                     FTDAAIGGDFNFIGLENFRKAFTVDPQFWVSFRNTILYVLMYVPANLVISFFLGYLLS
                     RNIKALGFFRTVFYIPYITSGVAVTVMWGWIFNGSYGLINYLLSLVGIQGPNWLADKR
                     YALICIVIISLWSIGNGIIIMLAGIQDIPATYYESAQIDGASSLRQVFSITIPLTTPT
                     IYFNLVIG"
BASE COUNT         3996 a         3955 c         3627 g         3853 t
ORIGIN      
        1 aagctgtacg agtcccctgc gggcagcata cggctggcag agggacggtg gtatggcgcc
       61 gtatatcctg gcggacgaaa aggatcttgt tcctcttccc gacgaactga cttataccga
      121 cggggcccag atcgcctgcg gattcggtac tgtatacgaa ggtctcgaaa aaataggcat
      181 ctccggaaac gatgcggtgc tggtcgtcgg tcttggaccg gtcggccttg caacgctgat
      241 gctcgccaaa gcaatgggcg cacagaaact cattggcgtc gatgtagtaa aggaacgctg
      301 tgagatcgct ttggataaga agctggcaga ccatgtattc atcagcggac cggatacgct
      361 ggagaaggtc ctggccctga caggcgggca cggagtggaa cgcgctgtcg acagctcagg
      421 ccatacatcc ggcaggcagc ttgcgatacg cgcaacaagg aaatggggcc gcatcgcatt
      481 cgtaggcgaa ggcgggacct gcgagttcaa tcccagcccg gatattatcc atgatcagaa
      541 gacgatttac gggtcatggg taacgagcat ctggaaaatg gaagagctca ccgaaaagct
      601 tgttcgttgg ggcatccatc cagaggacct tgtgacacac cgcttcactc tcgacaatgt
      661 tgcggaagcc taccgcctga tggcagaagg caaatgcgga aaagtcgccg tagtcttcga
      721 cgaggagata aaataagtgt cgtgccaggc acggcagacg gacagttatt cattgaaatg
      781 accctttgat attcggcgga catagatacc gaaagggttt tcaagggtca tcggcagtct
      841 tgggattact gtctgcctgt tgtgcctggc acccgatccc gtcgctcact caagcgtgaa
      901 cgacccgaaa tgggcgctgc cgtgcccttt ataagtaaag tacagggcgt gtactccgtc
      961 agggatcgct atgtctgcag agtattcctt ccagatgttc gagaactcga tcgggatcgt
     1021 gccgagcacc ggtccgtccc atttcgtcct tacctcgaag acgccccttg catatcctct
     1081 cgtttttatc tttatccttt ttactcccct gcagtcgaaa tacttgaatc ccgcagttgc
     1141 cgaatctttc atgtttgcta tatatccgat ctcctcatcc ccgtcttttc cgtcctgggt
     1201 tatcttcggg aactggttgt tcatccagga cgcggtccag tcggtataaa cggattcttc
     1261 gctgcagaag aggttgcagg caatgtatgt cggatattcc ccgcgtcccg cgagagggcc
     1321 tccgttgggg ccgcaggatg tcatttccgc ctgtattatg gatccgtcct cacggaacgt
     1381 tataggttcc aggcatgcct gcctgctgaa gttcgtaccg ttggtatgcc tgtggtagaa
     1441 aatataccac tggccgttta tctcaacgat gctgccgtgg ttgttgccgc catagaacat
     1501 tggtttgtcg gccggcttat atgtgtctat gcccaggtcg ctgttgctca cgataactcc
     1561 gcggtatgtg aaaccttttg tcggatattt gctggtggca tagcacagct catggaaaac
     1621 gacagaagag aatatgaaat agtatgtgtc tccccttttc cttatcgacg gtgcttcgaa
     1681 gaactcatgg ccctcaaatc cgctgccttt gctgtacggt tcgctcggag cgatgaaaac
     1741 cggttcctcg actattgtga gcatgtcggg gcccagcacc gtagccatgg gacctttcct
     1801 ggacttgtcg ccccttgggc agaatcccgt atacaggtat gtcctgtcgc cttcggtcag
     1861 cactcccggg tcgaactggg gctgatcgcc ttccctttca cccaggcgtg tcccgtcggg
     1921 ataatgtaca tagccgtaga attcgtactt gcccgcgggt ttgtcgcaga cggccacgga
     1981 caccacagga actttatcga gcacataata caggtaatac cgcccgtcgg gacctacagt
     2041 gacatcgggg gcatacaggc acatgctccc gtccgggttc agcggatcgt ccgtcttcct
     2101 gtagatcacg ccttcatagc gccagtcggt gagatcgtca acaggcgcgg accagcatac
     2161 ataatcgttc aggcagtaca catacccgtt gaagcggtca tgggacccgt agatatagac
     2221 cctgccgtca aaaacatacg gctccgcatc ggggatatac tcccaggaag gaagataggg
     2281 gttgaatccc tgttttttct ctgtcatcgc aaacactcct ttgattgttg tagatattat
     2341 attatataat cccgggattc atgcagtcaa acgtttgacc acatggtaca ggagagctat
     2401 tgctatatag cttatttcat tgccctgcag cgacaaatat agtcaaggca gcgaaatagt
     2461 gacccggaaa tcctcctggt aagggaaact gccgagtgcc gctccgaagg tcagttttct
     2521 cccgttttcc ctaccaggtg aatggcagcg taattgccgt tctcatatta gtaattggat
     2581 gccaggttca gtaaaaacct tatggacaac atggttctgc attgctaaaa taaatcatgc
     2641 gacagatgct atcgaaaagt tgatatttgg ccttttatca cctgccaggt tttgaagttt
     2701 ataaacagtt tattttaagt ctttaaaatg aatacgactc aggaatcgac ggaggggtga
     2761 taaatgcaga taaatctgct cgtaacgctg gattcgaagt acatcaggcc gttgaaggta
     2821 atgctggcgt cgatgttcat caacaatccg gaggagaatt ttcacgtcta cctggtccat
     2881 tcaagcataa aagatgagga aatagccgat ctcggacagt tcatggacgg tcgggggcaa
     2941 aaactgtcag tgatcaaaat agatgacggt tacttccagg atgcgccctt agtattccat
     3001 tatacaaagg agatgtacta ccggctgctg gcctataagt ttttgccgga agagctggac
     3061 cggatcctgt acctggaccc ggatatcctg gtactcaacc ccgtccgcgg attttacgaa
     3121 acagaccttg acgggtacct ttacgcagcg gctttccaca atgtgattgc agtaaaagga
     3181 ttgaaccaga tccgcctgta tccatacaaa atcaatgcat acataaattc gggcgtactg
     3241 cttatgaacc tggaactgca gcgaaagctc atagatgagg aagcaattta tacttttata
     3301 gaaaagaacc gcgcaaaact tttcatgcct gaccaggaca tcataaacgc gctctacgcc
     3361 gataaaataa agtgccttga cgagaagata tataattacg acgccaggtt ctacaactat
     3421 tataaaatgg taaccagcaa caagtgcgac atggactttg tcatcaggaa cacggtattc
     3481 atccatttct gcggcaagaa gaaaccctgg cgaaaaggtt acagcgggaa gttccattca
     3541 ttgtacaagc attatgagaa gctggcaata tcgtaaggct gcagaaacaa aatttggggt
     3601 ttatgcataa agctattatt ggaaggtatg atgggtaatg gcatgctgcg cagatgtacg
     3661 gcgttctgtc tactcaccat tggcaagatc ccgggtgttt tccctgatcc agttggtaat
     3721 atgatgatca gtgtccgccg gatacttaag atgcagttta tcagccgttt cgacagaaat
     3781 cctgcggaac agatcgatcg atgccagcaa gccccttaag ctgtctgccc tatcatacag
     3841 ggaaaaagtg gttttcaatt cctgcaatac cttttgatcg atccattcat ggaggaaccg
     3901 tcccgcatgc cagacatcat aatcattgcc attcaatgct tttgcatgcc attccatcat
     3961 ttggagcatc aatttcctga gatcattttc cctggctttg gctgcccata attcattgcg
     4021 tagaatctgc tttgccgtat acagggcgcc aaagaaaaac atactgacaa cctggacaaa
     4081 cgacctctca tcaatcgccg gagatgccgc gggcttaaaa tcgggcggta atatgtacct
     4141 gctcgtatta tccttgtcca ccaataccct tactcccctg cggaaattat acgggatcga
     4201 cctgtccttt accaaccggt acaggctgtc gctggccaaa aatacaatat cgacctgaaa
     4261 tccgccttcg aataaagtaa ggcgttccgg ctcgcctccc gaagtctggt atgcaaatgt
     4321 cacccaaatg tttccaagac ttttcagcca gtcaatattg tgcagataga aatcactgtt
     4381 atctgcatat agaataatat ccaggtcagc ccactgatcc gcaggatggt cggcacgggc
     4441 ccttgatccc acaacaaaag cagccctgat gtcatctgtc gctactgccc atgatgtaaa
     4501 atctttttca aaagtactgt aaaaatcatc cggtttcatc gcctgcaagc aatcctttcc
     4561 gttacgacgt tatcggcatc actgatcgta atatggcaac agaacgatat agtcagagta
     4621 atattatata aacgacagca caatatgtgt ttactatacc aaaaaagtaa atgactgtca
     4681 aaagcgatgc tttcactccc aggatcactt ctgaacagtc attcttttgc tgtaacctgg
     4741 gtcaacgtca cttaaatccc tccgtaagta tcaggctgta tcctggatac cctgctttga
     4801 agcgatacga tattgatcga tccactctgt ttactattat agttgccgtt tacgcaaaca
     4861 aaaaacaggc aagagagacc gggtttcccc cgatctgcat tcaaaagttc ccgttaagag
     4921 aagaatctta ataaaaaact atccggttgt tttctctcta aataatcaaa atgacttaaa
     4981 tgttggggaa ttgccgaatt atgccgttca ccatcacatc ggccatctcc agggcctgct
     5041 tttcgatttg gtcaaaaaca gttatgccct cagcatattt cccggtcagg atattgaccg
     5101 cttcagattt ggtcaatgcc aggtgttcac gcagcatggc ctgccattgc cgcgccgacc
     5161 agcaagggtt tatactgcca aggaaaacag cgatctcatc agcatttgca taccaccttt
     5221 tttctgcatc cgcagcagct cctgtatcac ctgcttttga tgcttttacc agttcggcgg
     5281 caataacaag atggctggta agcagatctg caaacctggc ggctgttcca tccccataaa
     5341 gcggcttcag caacgcctca aagtctttgg gattcctcag cagacggccg gtgaccggat
     5401 caacatcagg caaatcaaaa accatgctga tgatagtcag cctggtccag acaacgtgct
     5461 gctcccagag caaacgaagc tgattgttca gagcaagtcg tgatctgctg accatggttg
     5521 gggaaacata ctggcagggt aagaattgca cccctggcat atatgcataa ttataagggt
     5581 atgctcctgt agtacctgga tacataatca tcactgccta aatacttctt tgtatataat
     5641 atgattatgt aaacaaaatg tacctgtcac ggctttttcc ctttagcttt cgcttcccat
     5701 tcctctttca ggatagcgta ttgataacta tcccgccatt cgcctctaat gaaagctgat
     5761 tttctgaaac acgcttccct tcgcatcccg tttttctcca ggatcctttg cgacgcgata
     5821 ttgaaagtat cgcaattcgc ccttatgcgg tggagattca gtttctcaaa cccgaatttt
     5881 atgagagccg cggctgcttc agtaccatag ccctgtcccc aggctttctt cgagtagcag
     5941 tagccaatca ttccctgccg gttatcatat ccctcaagcg aaagttcgac agcgcctatc
     6001 aggctcccgg ttctcttctc tgtcacaacg agatggtatt ctttgcgagg gatcttctcc
     6061 ttttcactca atgccatgtc aacaaaattc cgggtgtcaa cttcggtatt gggtccccac
     6121 ggcatgaacc gtattacgtc ttcgtcagag gaatagtggt gtatttcctt ccagtcttct
     6181 tccctgaact cccttaaaat gagcctgtct gtttctatcg tgatatcagg attccggaca
     6241 ccgtctccac tgtccacggc atcaggagcg ccgtttcgtg cagcagcaga tccggcgtct
     6301 tccgtctcag gtttgctgcc tttccggact ttgcctttcg ccagcaaatg ttcgcccgca
     6361 tccgcaagat gcccgacaca gtaggataaa tcctcaatgt caagtatagt cttccacgca
     6421 gtttcatcct tttcaagcag ctctgcctgt ggtctcaatc ctgacatgat cgaaggagac
     6481 gctcccaact caatatcagc caacccggca tcctcaagca gttttttcag ttcagccgca
     6541 gtatataaat ggcgggaagg atgggcaaca tcaggatgta ttaagtctcc tgtcctggcg
     6601 acctcatgga ttttccagta ctcaggcctg ccccagaagt tttcggggac cattccttca
     6661 ttgacggcgc acgagcgcag tactccatac aaactgttga cgcttaaaag cagcacccca
     6721 ttgtttttcg tgaccctctt cagttcgtcg actgccttcc tgtcattgct gaaaaggtaa
     6781 ttcagcactg ctccgtagca aataaccgta tcgaaataac tatccgagat catatcaaga
     6841 ttcgtcacat cagccctgat ataccgctcc acctggacgt ccaggccccg ttcgtttatc
     6901 ttctgccttg ccaatgtgag ttggctttcc gatatatcca ggagggtcac tttgctgccg
     6961 ctttgagcga tatggatact gaacctccct ggcccgcagc cggcgtcaag cacccgtttc
     7021 ccgggtccta tatggggctc catgaattta acatggttat ggtaaaccag cctttgataa
     7081 gcagacttat ccaggcggtc ccattccttc tcgccataat cattatagaa ccggcgcacc
     7141 tgttcaggat caaacatatc aagcaccccg catcccgtat ttgataattt caataatttc
     7201 cccttcggtc tgtcctttgt cataatcacc tgttagctga tttttataat gatatgctat
     7261 gccgttttcg ttgtttagtt caggcagcgt atgagtgact cctttccatg atcgttccgc
     7321 atcacaaaac tcaagtctca tctcctgtgt atcttgattg cttccggacg aaccccggtt
     7381 tttcatatac aggcttgccg gcatcagtgg cggaaagttc aaaggcctgt gatccgtcac
     7441 ggcttgtcat caaaacttgc caatatatgt atataaaata gcagaaaatt aaatggccgt
     7501 caaaagaaat ttgacggcca ttgccggggg ggacttccgg caagtggatt actgtatgcc
     7561 gactttcctg cccgctgttt ccggtcttat caggacatta caggactttc tcgaccggga
     7621 cccggatctc gcagtaatag tcttccgggt tttcagcggg tggcgtatac atttcgatgt
     7681 tatagttccc tgcaagcttg taatccttgc agcttggcag ccactcgctc cagatcctcg
     7741 tgctgacatc cgcagtgatt tcggcagcgc gcctttgcac gggaataccg cctatgtccc
     7801 ggcaggtatc actttggtca cttgcccata acagtgaatt gagctttttc aagcggatat
     7861 cggacttctc cctatggatt tatatccata cgtgcctctg atttcagatg ggcagcttta
     7921 ttcagtccgg cctgtgcagg ttcctcccca ggtccgctta cagcacctgc tccctaacca
     7981 tcctcagata ctcccttatc ccgtcttctt cctcctgccg gtaggtaaag ccgaagtgcg
     8041 ccgccacctc gacagccagc gtatggaaca gatcgcacat agtatcaatg gctgcccaga
     8101 tatcggcata gtcactgccg gaataggtgg ctgcgtattg cgcataaagt tcctgaggca
     8161 gatatttctt aaagtacttg ccgctcttgc ccgtggaaag gctgaatcca cgctttgccc
     8221 cgatatagta gttgatcatg tcgtgcaatt catgccgtac cacttcattg agcatatgca
     8281 ttacataggg aagttcatct ctcgctatgc ccttggcgac attgttcaga caccaccaga
     8341 aattgttgca gcaggagtaa tagaatagag gcgacggcgg ctcgatatgc cagtgctcgt
     8401 cgctcggggc cggcaggggc ggaaggaacc ctctgccgtt gtccttgtcg agcaggacga
     8461 ccgcaggttc cccgttgtca atgtactttc tgtgatcgaa agtaatgtcg atacggactc
     8521 catcaggaaa gatcatcata taagcgaaag taccgtctcc ttcaggatag cgcatggctt
     8581 ccggcatctg catgatcaga ggcttgccga atcgttcttc cgcccacgcg ggatcgttcc
     8641 ggaagggcgt tatgtcctca acgaaatacg tgatatcgta atcctggtat ttatctttcg
     8701 gtacggcagg gttcgcccgg gaaccggaca tcagtactgc ccgtatgcgc tcatcagcct
     8761 gggccacgct taaaataaga tccatcattt cctgttcgct tcgcatgttc tccggtcccc
     8821 tgtctgtgag aattatatca atcggttata tggaagaagc agaactattg ctcaaacctc
     8881 tcttcttttt ttaaactccc ctgggggttc ataaggagcg atctctttgt aatacccgtc
     8941 atcaaagatg atcacatccc tgtgcatcgt cccttttgaa gagtcgtaat accatatcac
     9001 aagaggcgtt atcttataca gttcattgtt ttcgagttcg aaatagaatt tatagcccgg
     9061 gaatttcgcc aggtacttct ctttatattt caaatactcg ctgcttccgt attcgagttg
     9121 ctgcgccctc ccatggatct ggatattgtc gatgcatact gccacattgg gattgacgcc
     9181 gatgttcctg catttcaatg tatcagaaaa tgaaccgaag tacagatcga acccgtccga
     9241 ataatatgcc accatcgcat tgtcaacgtt attttcgccg gcagttgcta tatagcacat
     9301 tttcttttca gcaagcgctt tcaggacctt accctttatc ccgccgtttt gcatgctcat
     9361 tcaaacctcc tcagcatatt ataccataag tggccgccgg gttccccggt atattccagc
     9421 agatagtcag caacagtgat aatcatcgca cttgcggcag ccagtatgct gaaaagagta
     9481 tgttttctca gaatcatatc gcacctcgac cttcaggcaa aaccgcacat tgcccttctt
     9541 ctatagaaca gccggatgta aggtgcggct gtaaatgcac atgtttccat taatttctcc
     9601 gactaatcat atattacctt accagccttt gcaatcgcaa gactgctgca ggtatcagat
     9661 tcaaaaccct gagcagaata atatttgaac cgtcccttat cttgttacct gcgaaatcat
     9721 ggaatataat gaataatgca gttaagcatt cataaaataa cacacatcac aacaaggagg
     9781 ttgccatgaa gtactatgta gatgcaaatg caccaaaaga cggcgacggc tctgtcaaca
     9841 ggccgttcaa aagaatcagc gaagcagcaa aggtcgctct acccggcgac gaagtgctgg
     9901 tagccccggg gatctaccgt gaatacgtgg acccggtcca ttccgggacc gaagacgccc
     9961 gtatcaccta tgtcagcaca acgcctcttg gcgcggttat aacaggcgca gaacaggtaa
    10021 agacctggaa gcattaccag ggaaatgtct gggtctgccg gatcccgaac agcgttttcg
    10081 gcgattacaa tccgtatacg acgctggtat acggggactg gtatttcgct acgcctaaca
    10141 agcatacagg ctgcgtctat ctgaacgaca aggccatgta tgaggctgta actctggaag
    10201 aatgcatcaa gggcgaagtt tatgaatgca gttgggtacc ggaggattcc atttacaaat
    10261 ggtacaccga acaggaccag gatgccgatg aaacagtcat ttacgccaac ttccagggat
    10321 tggacccgaa taaggaaaat gtggagatca atgtacgccg caggtgcttc atgccttcca
    10381 aaaccggcgt cggttatatt accgtcagag gtttcaaaat caacaaagca gctaccacat
    10441 gggcgcctcc tgcagctttc caggacggca tgatcggccc tcactggagc aaaggctgga
    10501 taatcgaaga ctgtgagatt tcacacagca aatgcgcagg catttcattg ggcaaatacc
    10561 tggatcccga caatgagcag tatttcacgc ataaacacgt aaagagcccg acccagatgg
    10621 aacgcgacgc ggtctgccgc ggccagtacc acggatggct gaaggagaaa gtcggcagcc
    10681 atatagtccg ccgcaacaac atccaccact gcgagcaggg cgggatcatc ggccgcatgg
    10741 gcgccgtgtt cagcattatc gaggataacc atatccacca catcaacaac atgtcggagc
    10801 ttggcggtgc ggaaatcgcc ggtatcaaga tccacgcggc catcgacgtc atcatgcgcc
    10861 gcaaccatat ccaccattgc accatgggca tctggtgcga ctgggaagcc cagggcacac
    10921 gcatcacgca gaacctgctc catgacaacc aacgcccgcc gtatgcaaag ttcctgccgg
    10981 gcagcatgat gtgccaggat atattcgttg aagttggcca tggtccgact ttgatcgaca
    11041 acaacgtcct gctctccgat atcagcctcc gcattgcaac agagggcgtg gccatggtcc
    11101 acaaccttat ctgcggcgcg tttaccagcg taggcagcgg gacccatccg cgctatactc
    11161 cgtaccatat accccatcgc acggaagtga tgggcttcat gaccatactt cacggcgacg
    11221 accgcttcta caacaacatc ttcatccaga agtggcccgc cgacaagaag gacggggacg
    11281 aaaaagaagg ccaggacaac aggcaggtcg gcacgcacgt attcgacgaa tacccgacct
    11341 atgacgagtg gatcgcacag ttcgatttca cgaagcgtcc cgacatgatg ggactggcgc
    11401 ctgcccattc aggccatctg ccggtctggt gcgaaggcaa cgtatacctc ggcggcgcca
    11461 agccatggaa aaaggaaaca aaattccttg ccgtcgataa ctgcggccag gagcctaaag
    11521 tcgaacttgt ggaaaaagac ggcgagtatt accttgacac aaacatatat gatctcatcg
    11581 gcgatttcag cggccggatg atcaacaccg aagtcctcgg caaggctttc gagcctgaac
    11641 agtactttga aaatccggac gggacgccga tccgtttcga cgcagactac ttcggcaacc
    11701 accgcggtat ccgcgtcatt ccgggcccgt tcgcagcgcc cgctggtcat atcaagctgt
    11761 aaaaatggcg cctggcacgc cggttagtca gttatttcag ccggccatgc cgggcacgat
    11821 gtaataagaa tccttccggt atctgaattt acggatcacc ggaaggattt ttcaatgaat
    11881 agaacgataa tgtatggatg agaatttcgg atattataat ataataatcg cttatattta
    11941 ctgttggtta tcgcccgttt ttatgataaa ctattgaaag aaagcaataa attgaacagg
    12001 ctggtacggc ttatctgctc ttaaaccgcg gatccgggga ctgaaactgt ttgtcccgcg
    12061 gcatcggtgc aaaagagaga tcttatccgg caggtttaat cttacatacg agcaggcagg
    12121 aaaaacctgc agaatgagga gaggagtata tgctgtatca ggaaaccttt cagagtggag
    12181 aaaatccgtc ggaaatcagt gtcagcagtt atatttacat taatggcaag ctggaagata
    12241 acatgatcac agtcggtttt ccggaccatt gccattctta tttcgagata caatactttt
    12301 tttcaggatc aggagagaca cggatcgacg gaaaaccgat aaggacagtt cccaactcac
    12361 tgtacctgat accgcctctg cacgtacacg gacagtccgg gcatgagagg atggtcaatc
    12421 ttgtcataca gttcagccac accttcctta gcagcaatgc tttcgcatgg aagaaaaatt
    12481 caacgcttct gcctgccgga gaaatgaaaa aaagcggcag tatcatggtg gagcccggca
    12541 gcaggctgag caggtatatt gctgaaatcg aagaagtttc tcccaggttt gaaacctggc
    12601 tgcctgaaag cgaaaagaaa ttcgaataca cggtggagta tgaatggaaa ctccactcgg
    12661 ctacgctcgg tatcatcacc gaactgctgg atacaggaaa cctggtcatt tcggaaaact
    12721 ccggggacgt ttcggaaacg atcaagctcc atgcagttct taaccgcctc gtgacccatc
    12781 ctgaagaaaa acttgatatg aaaaaggcgg caaagcttgc atgcatgagc tactccaact
    12841 tcagccgggc ctttaaggag gccatcggac gttcatatgt ggattactgc aacagcctga
    12901 gagtgcacag agcggaggaa ctcctgaaaa caacgaagat ctccatcacc aatatttctc
    12961 aaatgctcgg cttcggatcc gtcagctact tcaacaggat attcagagca tatacgggca
    13021 gtacgcccat gcgataccgc aaagagtcaa tgtgatacca aatatttttt accgtcctgt
    13081 catgcaaaat cagactattt ttcaagagga tttttactag tataacaatt accccaagct
    13141 gtacaattta tatataagaa tggttctttt aactgtgttt attcagcaaa atccacaaag
    13201 aaaggagcca attcttaatt tgcccatgga tccgggcagc cggatgacaa agggcacaaa
    13261 atcacaaacc aagaaggggg ataaaagaga tgaaaaaact tttggcaatt cttctggcag
    13321 tagtcatgat tgtcatggta ggcgcttgcg gcaccaagaa taacggtgga acgacaacgc
    13381 cgaacagtcc tggcggcagc aacgaagaac ccggaacacc cgctcctggc ggcgaaagtg
    13441 acacactgac aggcgaactg gacatctggg catggggcgc ggatgacgaa gcaacagcaa
    13501 ggaaagacgc aatcgaagta ttcatcaaga accatccgaa cctgaaagtt acttacacag
    13561 tgatcccgac agcagaccag gtttgggacc agaagtcgtc agctgcactt gcttcaggat
    13621 ccgcaggcga tgtaatgcag atgagccctg actggtacgg catgaacacc aagtactata
    13681 tcgatctcaa cccgttagtt cagagagacg gtatagattt ggatgctgtc attactccgg
    13741 gcctcattga caggtattat gacgccgacg gaaaactgga aggtctgccg cttcacacga
    13801 actgcttcgt aatagcttac aacaaggata tgttcgctga agcaggcgtg cctgaaccta
    13861 aggaaggctg gaccctgcag gatctcgccg actggggcaa agcattcgca aagggatccg
    13921 gcgccaacca gacatatgcc atggcaaagc actggtgcat gaataacttc atgatctact
    13981 ccatgggcgg cattccgtat accgaggacc tgtcgactcc gaaaatgggc accgaggaaa
    14041 tcgtcaaagc actggggctc tataaggagc ttctcgacaa cggtataatc ccgaccgata
    14101 cagcgcagaa gaccattccg gcccagacac tgtttacatc cggaaaggct gcaatgtaca
    14161 cgatgggcgg atttgaagca gcccaggtta taagggacgc agaagaaaac ggcattaacc
    14221 ttggatttgc tcccatgccc tccgatccta acggcaaaga gatcaacgtc cagtatgcta
    14281 caggttgggc aattaccacg actgccaaga atgtcgacgc agcatgggct ttcctgaaag
    14341 aatcctcatt cgcaaatgac gaaatgggca cgatcaatgc aagggttggt atccctgcaa
    14401 acaggaagat cgcagaatcc gtctatgcac agacaaagat aggcaatgtc gaattcacca
    14461 acaactacta tgtacagcat atcagcaatg cccatatgaa cccgttcggc ggcactctgg
    14521 cttcctcggg caatatctgg acaactatgg ttgaggaagt taccctcaac ggtcaggatc
    14581 cggcagcagt cgtagcaaaa tacgctccac agatcgaagc tgagttcgca agctatgaat
    14641 tcaacgccaa gaaataaccg ctgaatcgac gttcagagca ttgagatgca gagggagcag
    14701 ggttgtctgc gccctctgca tcagtctctt tatacttgaa tgaggtgaaa tggatgaaat
    14761 taaaggaaag atcccttagt ctgaaaagaa gggaagccct gaacttctac ctgtatattt
    14821 ccccatggct gctgagtttt ctcgtcctga cgatatatcc gatgctggat tcgttcctgc
    14881 tgtccttcac ggacgctgca attggcgggg attttaactt catcggactg gaaaacttca
    14941 ggaaggcctt tactgtggat ccccagtttt gggtttcctt ccgcaatacc atattatatg
    15001 ttctgatgta tgtgcctgcg aaccttgtta tatcattttt cctcggctat cttctgagcc
    15061 gcaatataaa agcgcttggt tttttccgga cggttttcta tataccttac attacgtcgg
    15121 gcgtagccgt tactgtcatg tggggttgga ttttcaacgg cagttacggt cttatcaact
    15181 accttctttc cctcgtcggg atacaggggc ccaactggct cgctgataaa agatatgctc
    15241 tcatatgcat agtaataatt tcgctctggt cgataggtaa cggcatcatc ataatgctgg
    15301 cgggcatcca ggacatacct gccacctact atgagagcgc ccagatcgac ggagcatcga
    15361 gtctccgcca ggttttctcg atcacgatcc ctcttaccac cccgaccatt tattttaacc
    15421 tggtcatcgg c
//
LOCUS       DAOSCC010000026        15215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2673_length_15215_cov_4.684301, whole genome shotgun sequence.
ACCESSION   DAOSCC010000026 DAOSCC010000000
VERSION     DAOSCC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15215
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2673_length_15215_cov_4.684301"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLG72_02690"
     CDS             complement(<1..770)
                     /locus_tag="PLG72_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOJ79740.1"
                     /translation="MKIYVGVTDINWYNYLSNIQPDEVNFWQPGGKQVFKALEPNELF
                     LFKLHSPLNFIAGGGFFVRHSFLPVSLAWEAFENKNGTVDYYSFRQAIYKYRHTDRNT
                     EPDPQIGCIVLTTPFFFKRSDWIPAPEDWKPSIVQGKTYSTEDLIGRRLLKQVEEKLQ
                     NNNITTGSDELKICEANARYGSEQIIKPRLGQGAFRVLVTEAYQRRCAITGEKTLPVL
                     EAAHIKPYSQGGIHETNNGLLLRKDLHTLFDRGYITVT"
     gene            complement(1255..1743)
                     /locus_tag="PLG72_02695"
     CDS             complement(1255..1743)
                     /locus_tag="PLG72_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ79741.1"
                     /translation="MSDIIFKTENYVFSYRVAGILIHNGKVLLQKPTNDTGYAFPGGH
                     VEFGETNEETLIREFKEEIGADIRVKEIKWVAEIFFPWGNKHCHQICLYYTIELTDET
                     QIPLDGRFIAHEHIEERDFDIEFYWIPLDKVKNVEVYPSNAGELMGHINEGVKHFVYR
                     EN"
     gene            complement(2091..2954)
                     /locus_tag="PLG72_02700"
     CDS             complement(2091..2954)
                     /locus_tag="PLG72_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOJ79742.1"
                     /translation="MNRRTRYTVLILAVIAIAAVYRFSAGSSVKSAGESIPNHGMPAG
                     KHTDTEYRGVHISDIDISDELMLVNSDYKLDEDFAADLSSAYRMIPLSTSDIKINGTV
                     LEAVGKLFAEAEKMGYKDFFVNSGYRSFQKQKEIYDSAEDKSYVQEPGASEHQTGYAV
                     DIAYIGLSGEAFGKSPQGKWLFENAHKYGFILRYPENKTDITKISYEPWHYRYIGIPH
                     SYYCYENNLCLEEYISFLKNGGSYSMSIDKTSYTVYYATENAGSIDIPADKTCKASSD
                     NTSGYIVVVTG"
     gene            complement(3087..5726)
                     /locus_tag="PLG72_02705"
     CDS             complement(3087..5726)
                     /locus_tag="PLG72_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79743.1"
                     /translation="MNIFLQVTLKTLSRNKTRTLVTIIGIILSAAMLTAVTSFVTSLQ
                     EFMIDVGVYEEGDWHGCLYDVGPNKISELKDDPGILRVAACENIGYSALPNPKFGSSP
                     YIFVIGADSNFNSMMPVHITKGRLPEDTDEIILPVHLLKNGGTEFSIGERITLDIGVR
                     MYDNRILSQQYAFTANEDGTAREELVIRETRSFTIVGFYERPSFESFTSPGYTAITVM
                     DGTVREGETFDVYFKLKKPAGIYDFMREEEQKEGYAGSSYNDDLLMYIGVSKYSGFYT
                     VLYSLSSILIILIMAGSVSLIYNAFSISVSERTKQIGLLSSVGATHKQIRKCILYEAM
                     FVSAIGIPIGILSGLAGIGITLSIVGQRLATIYSGYAPYKLSLHVNIWTIVSAVLLAM
                     VTVLISAWIPARRATRITAIEAIRQTKDINVKAKDVRTSKLTYRLFGLEGMLARKHFR
                     RSRRRYRTTVVSLFMSIVLFISASSFCSYLKDSVTGVLGPRDYDISWVLNTQAYTEMT
                     PRQVYEMLAQTEGLTDSVYAFVEFGSTIVDNEDIFDEFKKRYRSQAAGSEENGEEDSD
                     VVELPVCVFIVPDEVFDGFASDEGINGADAVVMAYTEGIDTATNKYTKTKILRDTLSV
                     LDLRMNLAYEGPFPEDLTEEELEALLFKDISIDVGAVVDSLPFAINGLNFGCNVTVMV
                     PERCVPKYFADMEISKFVYYFFKGKDHAALADKLSAVLDEHGLPAGNLRDHADYEETN
                     RNMIFIINVFAYGFIVLISLIAAANVFNTISTGINLRRREFAMLKSVGMTQKGFHKMM
                     DYECLMYGTKSLLWGLPAAVGVTYLIYRSINSGYVTNFYIPWVSIIAAVFSVFAVVFT
                     TMLYSTNKLKNENIIDALRNENL"
     gene            complement(5723..6406)
                     /locus_tag="PLG72_02710"
     CDS             complement(5723..6406)
                     /locus_tag="PLG72_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79744.1"
                     /translation="MDILRIENLTKIYGTADNMVKALDSVSFTVKKGEFIAIVGPSGS
                     GKSTLLHIIGGVDKPTSGKVYMDGQDIYAQDDEQLAIFRRRQVGLIYQFYNLIPVLDV
                     RENITLPVLMDGKKVNEERLDELITFLGLKGREKHLPNQLSGGQQQRVSIGRALMNAP
                     AVVLADEPTGNLDSKNSHEIVELLKMSNKKYNQTLIIITHDENIALQADRIIALEDGR
                     IIRDEVIRK"
     gene            complement(6606..7613)
                     /locus_tag="PLG72_02715"
     CDS             complement(6606..7613)
                     /locus_tag="PLG72_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ79745.1"
                     /translation="MDILRNPWVKKNVLIHLALGAAATVTGFLIGTACGILALCLCLL
                     FTLVHYLSDRKRYRKISELSDEINKILHSRHTLDINEYAEGELSILSSEIYKMTVRMR
                     EQAELLEKDKQYLVDSMANISHQIRTPLTSVNLIATLLQKPDLTYERRLQLTQDMMKL
                     LEHIDHLVTVILKLSKFDAGTVELKKETVSVADLISKASEPFLVPMELRNQQLCVNIN
                     GNASFSGDLAWTAEAVGNILKNCMEHTPEGGVIKIDASENALFTEIVISDSGSGIDRD
                     DLPHIFERFYRGKNASGQNYGIGLALARLIISEQNGTIKAENAAGGGARFTVRFYKSV
                     V"
     gene            complement(7689..8360)
                     /locus_tag="PLG72_02720"
     CDS             complement(7689..8360)
                     /locus_tag="PLG72_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ79746.1"
                     /translation="MTRILLVEDDKSIVDNLTEFLKSEGFSVESVSGQAAAIRKIEND
                     SYDLLLVDISLADGNGFAVCAAAKANTNIPVIFLTASGDEYSVVTGLDMGADDYIAKP
                     FRPRELVSRIKNALRHSGKAQSVIELEGLRVDTVKGTVTKNGSEIFLSALEYRLLLVF
                     LNNRGTVLSRSKLLENIWDIAGEFVNDNTLTVYIKRLREKIEDDPQNPKIIKTVRGIG
                     YKVDD"
     gene            complement(8460..9284)
                     /locus_tag="PLG72_02725"
     CDS             complement(8460..9284)
                     /locus_tag="PLG72_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ79747.1"
                     /translation="MIGAIVGDIVGSRFEFNNYRNKNFELFTGDCKVTDDSIMTLAVA
                     KAVMEARRIRKSAPDGCDSEYYAAAERLAVKYMQQIGRRYPNCGYGGRFIHWVFSDTP
                     QPYNSFGNGAAMRISPVGFASETEEEVIRLSRAVTGVTHNHPEGIKGAEAVAMAIFMA
                     RKGYSKKEIRDRIVKDYYSLDFTIDEIRSTYEFNETCQNTVPQAIEAFLEAESFEDAI
                     RTAVSVGGDSDTIAAITGSIAEAYYGVPEDVKNRALTYLDVDLRNILEEWYSFSYN"
     gene            complement(9338..9757)
                     /locus_tag="PLG72_02730"
     CDS             complement(9338..9757)
                     /locus_tag="PLG72_02730"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOJ79748.1"
                     /translation="MKYAGIIRRGVAIVLDFIFFCIVFFPITYMVKGVWLMQPEDHLW
                     IIFDPICGVFLVIIILYYILLEGITGYTLGKLIVGIRVRDPDGGRITLKQSVLRNLAR
                     LVDGIFFNLVGAVIIYKSPLNQRLGDKIAKTVVCRIR"
     gene            complement(9892..10278)
                     /locus_tag="PLG72_02735"
     CDS             complement(9892..10278)
                     /locus_tag="PLG72_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ79749.1"
                     /translation="MSLVPKSIILKSEILTDTETALMKQHCELGMSSLVSIGFPRKYL
                     DVVAQHHEMLDGSGYPKGLKGDEISRNAKISIVANADDDISSARPGMQAQPMEAVIWH
                     LKTENEKYPQEYVEVLEEVLGFRHAN"
     gene            10896..11180
                     /locus_tag="PLG72_02740"
     CDS             10896..11180
                     /locus_tag="PLG72_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79750.1"
                     /translation="MFFARAPDVDGDIKGIDLIPGAEPEACGIFLRIGHHDLNGVRKG
                     IFLSSCIAKLFIKFRLIKTKKAKAPDSFKLLQFCLSYTNIKKMIIDIPLG"
     gene            complement(11301..12236)
                     /gene="cysK"
                     /locus_tag="PLG72_02745"
     CDS             complement(11301..12236)
                     /gene="cysK"
                     /locus_tag="PLG72_02745"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ79751.1"
                     /translation="MARIYKNLTDLIGRTPLLELTNYGKKHKLGATVIGKLEYYNPAG
                     SVKDRIAWAMIDDAEKRGVLKPDSVIIEPTSGNTGIGLASVAAARGYRIILTMPETMS
                     IERRNLLKAYGAELVLTEGSKGMPGAIAKAKELAAEIPNSFIPGQFDNPANPRIHRET
                     TGPEIWEDTDGKIDIFVSGVGTGGTITGAGEYLRSKNPNIKIVAVEPDASPVLSGGNP
                     GPHKIQGIGAGFVPAVLNTNVYDEIIRVKNEDAFETGRELARTEGLLIGISSGAAVWA
                     ATELAKRPENAGKVIVAILPDTGERYLSTPLFSEQ"
     gene            12573..13019
                     /locus_tag="PLG72_02750"
     CDS             12573..13019
                     /locus_tag="PLG72_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ79752.1"
                     /translation="MRISAKGRYALAAVINMAQQYDSGECITVISISERLGISKIYLE
                     QVFSMLKRGDIVRSVKGAQGGYQLVRPPEQITVLDVLSAVETSLFEAVEDTVPEKAPE
                     IEAAMRSSVFEAMDRSLSDALSRITLEDLVLETEKYSGGQSIMYYI"
     gene            13270..14406
                     /locus_tag="PLG72_02755"
     CDS             13270..14406
                     /locus_tag="PLG72_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOJ79753.1"
                     /translation="MEFETLCIHGNDTRYDSTGAISVPIFQSATFAHPGVGLSTGYDY
                     SRLQNPTREHLEKTIAKLENGIDAMAFSTGMAAIAALMELFSPGDHFIASDDLYGGSH
                     RLFCHISGKNGLSFDFADTSDIEKVSSLIRPETKAIFVETPTNPMMQVTDIAAVSELC
                     KEHGLLLVVDNTFLTPCFQKPLDLGADIVVHSGTKYLGGHNDTLAGFLAVSDAALSER
                     LRFIYKTTGACLSPFDSWLMIRGVKTLAVRMERQQENAMKIAQWLCTHKKIKKVYYVG
                     LPSHPQYEISKKQTTGFGAMISFETDSEKTALGLLQRVNVIQYAESLGGVESLVTYPM
                     LQTHADIPKEAREAKGINERLLRLSVGLESAADLIADLEQALGD"
     gene            14492..>15215
                     /locus_tag="PLG72_02760"
     CDS             14492..>15215
                     /locus_tag="PLG72_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ79754.1"
                     /translation="MTYSFDKVIDRHGTDSLKYDLAAKRGLPEDILPLWVADMDFPSP
                     PEVLEALVDKSLHGIFGYSDAINDDYYNALADWYASRFGWHLSPKWLVKTPGVVFAVC
                     TAIRALTNEGDSVLIQQPVYHPFAGSVLANDRKLVVNELVYNDGKYSIDFDDFEDKIV
                     KNNVKLFILCSPHNPVGRVWTKDELAAMGEICLKHGIKVVADEIHADFVYPGYRHHVF
                     ETVRPEFSDITVTCTAPTKTFNL"
BASE COUNT         3922 a         3802 c         3216 g         4275 t
ORIGIN      
        1 tcggtaactg taatgtatcc cctatcaaac agagtatgca aatcctttct cagtagtagc
       61 ccattatttg tttcatggat acctccttga gaatatggtt tgatatgagc agcttccagt
      121 acaggtaacg ttttttctcc agttattgca catcttcgtt gatatgcttc agtaaccaat
      181 actctaaaag caccctgccc aagacgaggc tttattattt gctctgatcc atatcttgca
      241 tttgcctcac aaatttttaa ttcatcagag cctgtagtaa tattattgtt ctgaagcttc
      301 tcctcaacct gtttgagcag tcttctacca attaagtcct cagtgctata agtttttccc
      361 tggacaatac ttggcttcca atcctctgga gcaggtatcc aatcagaacg tttgaagaaa
      421 aagggagtgg tcaatacaat gcagccaatt tgcgggtctg gctcagtatt tcggtcggta
      481 tggcggtact tatatatagc ttgacgaaag gaataataat caactgtacc atttttgttc
      541 tcaaatgctt cccaagccaa ggaaacaggc agaaacgaat gacgcacaaa gaacccacca
      601 ccagcaataa agtttagtgg actatgtaat ttaaataaaa agagttcatt tggctcaagt
      661 gctttaaaca cctgtttccc accaggctgc caaaaattta cttcatccgg ctgaatgttt
      721 gatagatagt tgtaccagtt tatatctgtg accccaacat atattttcat tgtatcacct
      781 aatactagaa ggtttgtcaa tgatatttta ctactcttac atttatttga aaagatagac
      841 aatgatactc aataatttaa ttccttcagc caaatgcaaa tttaaatcat gttctcctac
      901 tttaaggtat atgcattttt tgaatgtatt aaaacatact catattgctc caaaccctgc
      961 tgtattaacg ctttacctcc tatatagcac tgttatcgtc tccacatggc acaagttgat
     1021 gggattgatg tcaatgggcc agtttttata gtatctgttc gcgggaacat atctatatac
     1081 gtgtctattt aacctttata aagttataaa tatatccttt tttacatcct caaattttaa
     1141 actatcgtta tgtattttcc attgataggg actgatcaag ttccagcgaa tcgcttcgcg
     1201 tagatcagct atctgcattg cagaagtcgg agcatttaca acataattca tatgtcaatt
     1261 ctccctatat acaaaatgtt taacaccttc attaatgtgc cccatcagtt caccggcatt
     1321 gcttggatat acttctacat ttttaacttt atcaagagga atccaataga attcaatatc
     1381 aaaatctcgt tcttcaatgt gctcatgagc aatgaatctt ccatcaagcg gtatctgcgt
     1441 ctcatctgta agttcaattg tgtaataaag acaaatttga tggcagtgtt tattgcccca
     1501 tgggaagaat atttcagcaa cccacttgat ttcctttacc ctaatgtctg caccgatttc
     1561 ctctttgaat tcacgaatta aagtttcttc atttgtttca ccaaattcta catgcccgcc
     1621 gggaaaagca tatcctgtgt cattggttgg tttttgcagc agtaccttgc cattatggat
     1681 aagaattccg gcaacgcggt agctgaacac gtaattctct gttttgaaaa taatatcgga
     1741 catattactc aacctcactt tcaactatgg aaaaatgaat ttttttattc aagtacaaaa
     1801 cctattaaac tagatttaac tcattatttt tcctatctcc tcaaccctct caaaaatgcc
     1861 cgttatctat tctgtcaact caactctacc cattttcggc ctcatatttt atccacaaat
     1921 acaccttcga tatgttcccg tgtacgcatt ttacctcatt aggtcgagta gataacttgg
     1981 atgtaattcc tgaaactcgc ttaaataaat acttacagct ttgtaatcaa tactacacac
     2041 tccacgtgtc ctggtcatcg tcacatatca atatgctata ttccaaaaac tcaccccgtt
     2101 acaactacga tatacccgct tgtgttatct gacgaggcct tgcatgtttt atccgccgga
     2161 atatcgatgg aaccggcgtt ttcagttgca taatatacag tatagcttgt tttgtcaata
     2221 gacatggagt aactgccgcc gtttttcaga aagctgatat attcctcgag gcacagattg
     2281 ttttcatagc agtaatatga atgtggaatg ccgatatatc gataatgcca tggctcataa
     2341 ctgattttcg ttatatcggt cttattttca gggtatctca aaatgaaacc gtatttatgt
     2401 gcgttttcaa aaagccattt cccctggggt gatttcccga atgcttcgcc gctcagcccg
     2461 atatatgcta tatcgacggc atatcccgtc tgatgctcgc tggcgcctgg ctcctgcaca
     2521 taacttttgt cttctgcact gtcgtatatc tctttctgct tctgaaaaga tctgtacccg
     2581 ctattgacga aaaagtcttt ataccccatt ttttctgcct ccgcgaacaa tttgccgacc
     2641 gcttccagga cggtaccatt tatttttatg tcacttgttg acagcgggat catcctgtat
     2701 gccgaagaca gatcggcagc aaaatcttca tcgagcttgt aatcggagtt tacgagcatg
     2761 agttcatccg atatatcgat atcagatatg tggactcccc tgtattcggt atctgtatgt
     2821 ttcccagccg gcattccatg attcgggatg gactctccgg cacttttcac gctgctgccg
     2881 gctgaaaacc ggtatacggc agcaatagct atgactgcaa ggatcaatac cgtatacctg
     2941 gtccttcgat tcattctttg tcctggtctt ctgctgttcc gcaacataca aacacacctc
     3001 ttcgttggtt ttattacatt atgaagaggt gtgtcgaagt tgaaggttat aaaaagttaa
     3061 gaattttcaa atatttctgt cgtccatcac aggttttcat tcctcagcgc gtctatgata
     3121 ttttcatttt tcagtttgtt cgtggaataa agcattgttg taaagacgac cgcaaacacg
     3181 ctgaacacgg cagcaatgat cgatacccat gggatataga agttcgtcac atagcctgaa
     3241 tttatgctgc ggtaaatcag ataagtgact ccaactgccg caggcagccc ccacagcaaa
     3301 gattttgtcc catacatcag gcattcataa tccatcatct tatggaagcc cttttgcgtc
     3361 atgccaacgg atttgagcat agcaaattcg cgccgccgca ggtttatgcc ggtcgatatc
     3421 gtattgaaca catttgcggc cgctatcagc gagatcagga ctatgaagcc ataggcgaac
     3481 acgttgatta tgaatatcat attcctgttt gtttcttcat agtctgcgtg atcacgcagg
     3541 tttcccgccg gcaggccgtg ctcgtccaga acggccgaca gtttgtccgc aagggcggca
     3601 tggtccttcc ccttgaagaa gtaatagacg aatttcgata tttccatgtc ggcgaaatac
     3661 ttgggaacgc agcgttccgg aaccatcacc gtaacattgc acccgaagtt gagtccattt
     3721 atggcaaacg gcaaagaatc gacaaccgcc ccgacatcta tagatatatc cttaaacaac
     3781 agcgcttcca gttcttcttc tgtcagatcc tcgggaaaag gcccctcata cgcgaggttc
     3841 atccgcagat ccagtacgga taacgtatca cgcagtatct ttgtcttagt atacttgttc
     3901 gtcgctgtgt cgatcccctc ggtatatgcc ataacaactg cgtccgcgcc gtttatgcct
     3961 tcatcggacg caaatccgtc gaaaacctca tccggcacta tgaatacgca tacgggcaac
     4021 tcaaccacat cactgtcctc ttcgccgttt tcttcgctgc cggcggcttg actgcgatag
     4081 cgtttcttaa attcatcgaa gatatcttca ttatcaacga tcgtgctccc aaactcgaca
     4141 aaagcataaa cagaatctgt caggccttct gtttgggcca gcatctcata cacctggcgc
     4201 ggggtcattt ccgtataagc ctgggtattt aacacccagc tgatgtcata atccctgggt
     4261 cccaatacac ccgttacact atccttcaga taggaacaga atgaactcgc agatataaac
     4321 agcactatgc tcatgaacag ggatacgacg gtagtccggt accttctcct gctgcgcctg
     4381 aaatgcttcc tggcaagcat gccctcaagg ccgaataacc tgtaggtgag ctttgaagtg
     4441 cgtacatcct tcgcttttac attaatatcc ttagtctgcc ggatcgcttc tatggctgtt
     4501 atgcgagttg cacgccttgc agggatccac gcggaaatca ggacagttac cattgccagc
     4561 agcactgctg aaactatcgt ccagatattg acatggagcg aaagcttata tggcgcgtat
     4621 ccgctgtaaa tcgttgccag cctttgtccg actatggaca aggttatgcc tataccggcc
     4681 aatcccgaaa gtataccgat cggtatccct attgcactga caaacatggc ctcatacagt
     4741 atacatttcc tgatctgctt atgagttgcg cccactgatg acaggagccc gatctgcttc
     4801 gtcctttcac ttacagatat ggaaaaggcg ttgtagataa gggaaacaga accggccatg
     4861 atcagtatga tcaggatgga cgacaggctg taaaggacag tatagaaacc ggagtatttc
     4921 gatacgccga tgtacataag caaatcatcg ttgtatgagc tgccggcata gccttccttt
     4981 tgctcttcct ccctcataaa atcatatatg ccggcgggtt ttttaagctt gaaatataca
     5041 tcgaaggttt cgccttccct cacagtgccg tccattactg tgatggcagt atatcccgga
     5101 gacgtgaagg actcaaaact cggacgttca taaaaaccga ctatggtgaa gctgcgggtt
     5161 tcccgtatga caagctcctc acgcgccgta ccatcttcat tagctgtgaa ggcatattgt
     5221 tgactgagta tcctattatc atacatccgc acaccgatat ccagggttat cctttctccg
     5281 attgaaaact ctgttcctcc gtttttaagc agatgaacgg gcaatatgat ctcgtcggta
     5341 tcttccggga gtctgccctt tgtaatatgg acaggcatca tcgaattgaa gttgctgtct
     5401 gcgccaatga caaaaatgta gggcgaagac ccgaattttg gattgggcag ggcggaatag
     5461 ccgatattct cgcatgccgc aacccgcagt attccagggt catccttcag ttccgagatt
     5521 ttattcggcc caacatcata aaggcatcca tgccagtctc cttcctcata tacccccaca
     5581 tcgatcataa attcctgaag gcttgtaaca aaggatgtca cagcggtcag catcgccgcg
     5641 gacaaaataa tgccgattat tgtgacgagg gtgcgcgttt tgttccttga cagcgttttt
     5701 aacgtgacct gcagaaaaat attcatttcc ttatcacctc gtcgcgtatg atcctgccgt
     5761 cttcgagcgc aattatgcgg tcagcctgca atgctatatt ctcatcgtgg gtaattatga
     5821 taagtgtctg gttgtacttc ttattcgaca ttttcagcag ttcaactatc tcgtggctgt
     5881 ttttggagtc aaggtttcca gtcggttcgt ctgccaggac aacagccggc gcgttcatca
     5941 gcgccctgcc aatggaaaca cgctgctgct gtcccccgga aagctggttg ggaagatgtt
     6001 tttccctgcc tttcaggcca agaaatgtta taagctcatc caggcgttct tcattgactt
     6061 ttttgccgtc catcagcacc ggcagcgtta tgttctcccg gacatccaaa acagggatga
     6121 gattataaaa ctggtagatc agcccgacct gcctccggcg gaagatcgca agctgctcgt
     6181 catcctgggc atatatatcc tgtccgtcca tgtatacctt cccggacgtc ggcttgtcca
     6241 ctccgccgat tatatgcaaa agagtggatt tgcccgatcc cgagggaccg actatggcga
     6301 tgaattcacc ctttttcacg gtaaagctca cgctgtcaag cgcctttacc atgttgtcag
     6361 cagttccgta aattttggta agattctcga tcctcaatat atccatcatt atcctcctct
     6421 gcgtttatgg atatatcata tcgctgccag gtgacactgt cgtgacttta ttattacaaa
     6481 aatgtcacct gggcttgcct tacgatcgcc acccgggctt gtttagctca tgcaatataa
     6541 atcccggtca acgcttggga taagtctgcg cttaagcctc tgaaacttgg actgcgcccc
     6601 tggccttata ctacgctttt ataaaacctg actgtgaatc tcgcaccgcc gccggccgca
     6661 ttttcagcct ttatagtgcc gttttgctca gatatgatca ggcgcgccag tgccagcccg
     6721 atcccgtaat tctggccgga agcgtttttc ccgcgataga agcgttcgaa aatatgcggc
     6781 aggtcgtccc tgtctatccc gctgccgctg tcagatatga ctatttcagt aaacagggca
     6841 ttctcagaag cgtcgatttt gattacgccg ccttcaggcg tatgctccat gcaatttttc
     6901 aatatgttgc cgactgcctc ggcagtccat gcaaggtcac cggagaaact cgcgttgccg
     6961 tttatgttta cacaaagctg ctggttcctc agttccattg gtaccaggaa cggttccgaa
     7021 gctttgctta taaggtccgc tacagaaacg gtttcctttt taagctctac cgtcccggca
     7081 tcgaatttcg aaagcttaag aataacggta acaagatggt caatatgctc cagcagtttc
     7141 atcatatcct gtgtaagctg caggcggcgt tcatacgtta aatccggctt ctggagcaac
     7201 gtggcgatca ggttcacaga agtgagcggc gtccttatct gatgcgagat attcgccatg
     7261 gaatcgacaa gatactgctt gtctttctcc aggagctccg cctgttccct catgcggacc
     7321 gtcatcttgt agatctcgct gcttaggatc gacagctccc cttcggcata ttcattgata
     7381 tcaagggtat gccgggaatg gagtatcttg tttatctcat cgcttaattc agaaattttc
     7441 cggtatctct tccgatcgga caaatagtgg accagggtaa aaagtaagca caggcacaga
     7501 gccaggatgc cgcatgccgt tccaatcaga aatccggtta cggtcgccgc cgctccaagt
     7561 gcaagatgta tcagtacatt ctttttgacc caggggttcc gtaaaatgtc catttattcg
     7621 cctgccttca tttgcctgtc ctgatcagac gcctgcatta taccgacgtc cccgctcacc
     7681 tgtactgttc agtcgtccac cttatacccg atgccgcgga ccgtttttat gatttttgga
     7741 ttttgcgggt catcctcgat tttttcacgc agtctcttta tatatacggt cagcgtattg
     7801 tcattgacga actcccctgc gatatcccag atattctcaa gaagcttgct cctggaaagc
     7861 acggtgcccc tgttgttgag aaacaccagc aataagcggt actcaagcgc cgagaggaag
     7921 atctcggagc cgtttttcgt tactgttcct ttcactgtat ccacacgaag cccttcgagt
     7981 tctatgacag actgcgcttt ccccgaatgc ctcagggcgt ttttaatgcg cgacacaagt
     8041 tcccggggcc tgaacggttt ggcaatatag tcgtcggccc ccatatccag accggtaact
     8101 acgctgtatt catcgccgga agcagtaagg aagatcacgg ggatattcgt attggccttg
     8161 gcggctgcgc atacggcaaa accgtttcca tccgccagcg atatgtcgac aagcagcagg
     8221 tcgtagctgt cgttctcgat tttccggatc gcagcggcct gcccgctgac tgattccact
     8281 gaaaagcctt cgcttttgag gaattcggtc agattgtcga ctatgctttt atcatcttca
     8341 acaagcaata tccttgtcat tggtcacctc ggccatatcg gacactgaac tctgcattaa
     8401 aactataatt ccgtaatgtg cttggatgca aggaatacag ctcttagctg ccttaacaat
     8461 taattatacg aaaaactata ccattcttct aaaatgttgc gcaaatcaac gtcgaggtac
     8521 gtgagtgccc tgtttttaac atcttccggc acgccatagt aagcttccgc gatcgaaccg
     8581 gtaatggcgg caatcgtatc gctgtcaccg ccaactgaga cagccgtcct tatggcgtct
     8641 tcaaaggact cggcctccag gaaagcttcg atcgcctgtg gaactgtatt ttgacaggtc
     8701 tcattgaatt cgtatgtgct cctgatctca tcgatcgtga agtcaagtga gtaatagtct
     8761 tttacgatcc tgtcgcggat ctcttttttg ctgtatcctt tccttgccat gaatatggcc
     8821 atggcgactg cttctgcgcc cttgatccct tccgggtggt tatgggtcac accggtaaca
     8881 gcccgggaca accggatcac ttcttcttca gtttcagaag cgaaccctac cggactgatg
     8941 cgcatcgcag caccattacc aaagctgttg tacggctggg gcgtatcgct gaagacccaa
     9001 tggatgaacc tgccgccgta gccgcaattc ggataccttc gcccgatctg ctgcatatat
     9061 ttgacagcca gcctttcggc agcagcgtag tattcactgt cgcagccatc cggtgcgctc
     9121 tttctgatgc gcctggcttc catgacagct ttggcaacag caagtgtcat tatactgtca
     9181 tctgttactt tgcagtcgcc cgtgaacaac tcaaagtttt tattgcgata attattgaat
     9241 tcaaaacgcg aaccgacaat atcgccaacg atcgctccaa tcatctattc atgacctcgt
     9301 ccatattatt gattttccca gtaacaatac tgtaatttta acgtatacgg catacgacag
     9361 ttttggctat tttatctcca agccgctggt ttaaaggtga tttatagatg ataacagcac
     9421 ctaccaggtt aaaaaatatg ccatcgacca atctagcgag attcctcagc acagactgct
     9481 tcagcgttat tctgcctcca tcgggatccc ttacccttat gcctacgatt agcttgccca
     9541 gtgtatagcc agtaatacct tcgagcaata tgtaatataa gatgataatc accagaaaca
     9601 ctccacaaat gggatcgaat attatccaca aatgatcctc gggctgcatc agccagacac
     9661 ccttgaccat gtatgtgatc gggaagaaaa cgatgcaaaa gaatataaag tccaatacta
     9721 ttgccacacc tcttctgata atgcctgcat acttcatatg gctccccttc tctgcagcag
     9781 gtctccaaat aagactttac atctaaagtt tattatatag aaagcaagat ttacattcaa
     9841 ttaagcggct tatcaattcc acgatcataa tacattaact ccggcaacag cctagttggc
     9901 atgcctgaaa cccagaactt cttccagcac ttcgacatac tcctggggat atttctcgtt
     9961 ttcagttttt aagtgccata tgactgcttc catcggttgt gcctgcatgc caggtctggc
    10021 agaactgata tcgtcatcgg cattggcaac gatcgatatc tttgcgttcc ggctgatctc
    10081 gtctcctttc agccctttcg gatatccgct tccgtcaagc atttcatgat gctgcgcgac
    10141 aacatccagg tattttctcg gaaaaccgat gctcacaaga gaactcatgc ccagttcaca
    10201 atgttgtttc atcaatgccg tttccgtgtc tgtcaaaatt tcagatttca gaatgattga
    10261 ctttggcaca agggacataa agttttataa tatttccctc ttttatatat ctttagtcct
    10321 aacctttcga aaacctcggt aatttttacg tttttcttta tttcattata ataatcattg
    10381 aaagtataat cactaattgt aatcatctcc ttcaagctta cagtatacgt gtaaatttat
    10441 tacataattc gacgtaaaaa ttaaagttcc ttcataagtt caattatctt tgcataaaat
    10501 tgttgtatga taatttttaa gggtattttt ctaaatgtta accatataat gttaacccga
    10561 tattagtatt cagtgtctgc ctctcgtacc attactcata ataacggccc actgtttaga
    10621 tacaagctat gatcccaaca agtttttctg catataagta cataaatgtt ctatactaaa
    10681 tcaattcaac aaaaattcaa aaaaatccta ttacttagca aacgaacagg aggcaggtgc
    10741 ttgtttcacc tgcctcctgt tcgtttgtcc catatgattc ctttattgta tatgtcaggg
    10801 tgcgtgagtg tacacgtcat cctagcacgg cttcgacaaa cctttgtata atagccaccg
    10861 gcagccagat agttatcgca tagcagggag cgggtttgtt tttcgctcgt gcccctgacg
    10921 tagatggaga tataaaaggg atagacctca tccccggcgc tgagcccgaa gcttgcggca
    10981 tcttcctgcg tattggtcac catgacctca atggagtgcg aaaaggcatt ttcctttcat
    11041 catgtattgc caaacttttt atcaaattcc ggctcataaa gacgaaaaag gctaaagctc
    11101 ctgactcctt caaattgctt cagttctgtc tttcgtatac caacattaag aaaatgatca
    11161 tagacatacc gttaggataa ggctcttcat gaaaaaaagc tgtctcctca atgggacagc
    11221 ttttttattc attaagtatg acttacatta tcacaaaatt cgaataagtt aaatcttaac
    11281 ctccaaaaat ccatccccca tcactgctca gagaacagcg gtgtcgacag gtagcgctcg
    11341 ccggtgtcgg gcaggatcgc gactatgacc ttgccggcgt tctcgggacg ctttgcaagc
    11401 tcggtcgctg cccagactgc cgcgccggag gaaataccga tcaggagtcc ctcggtacgg
    11461 gcaagttcgc gtccggtctc aaacgcatct tcgttcttca cgcggatgat ctcgtcatat
    11521 acatttgtgt tcagaaccgc cggaacaaag cctgccccga tgccctggat cttatgaggc
    11581 cccgggttgc cgcccgacaa taccggagaa gcgtccggtt ctactgccac tattttgata
    11641 ttcggattct tcgacctgag atactcgcct gcgccggtta tggtaccgcc ggtgccgact
    11701 ccggaaacga aaatatcgat cttcccgtca gtatcctccc agatctcggg acccgtcgtc
    11761 tccctgtgta tccgtgggtt ggccgggttg tcgaactggc ccgggataaa ggaattcggg
    11821 atctccgccg caagctcctt tgcctttgcg attgcgccgg gcattccctt ggaaccctcg
    11881 gtaagtacca gttcagcgcc gtatgccttc agcaggttcc tgcgctcaat gctcatcgtc
    11941 tcaggcatcg taagaataat acggtaacca cgcgctgcgg cgactgacgc aaggccgata
    12001 cctgtatttc cgctggtcgg ctcgataatg accgaatcag gcttcagcac ccctcttttt
    12061 tcagcatcgt cgatcatcgc ccaggcgatc ctgtccttca cgctgccagc cggattgtag
    12121 tattccagct ttccaatgac tgttgctcca agcttatgct tcttcccata gttggtgagt
    12181 tccagcaaag gcgtccttcc gataagatcc gtaagattct tgtaaattct cgccataaca
    12241 catcctcctt aacctaaaca tatccttata ttaatttcat tacttcacaa tctgcatcgt
    12301 tccgtcttct acaattatag cctcatttat tacagccctg cttcaaggcc tcgtttctgc
    12361 taacctgtgt tgtctcttcc ggttcagtca atcgaacata taattcatat atgtttagta
    12421 tgctataata atacagcacg acgaaatatt tgtcaatagt tttatcggaa aaatatataa
    12481 aaaatatatg aattgtatgc tattgacaaa cacagtattt tagcctatca tatttaatac
    12541 atactaatta tatatgaaat gaggagtttg ccatgagaat ctccgccaaa ggacgctatg
    12601 cgctggcagc cgtaataaat atggcgcagc aatatgacag tggtgagtgt atcacggtca
    12661 tcagcatttc ggaaaggctg ggcatctcaa aaatttacct cgagcaggtc ttctcaatgt
    12721 tgaagcgcgg cgacatcgtc cgatcggtca aaggtgcgca gggcggctat cagctcgtcc
    12781 gtccgccgga acagataacc gtgctggatg tgctgtcagc cgttgaaacc tctttattcg
    12841 aagcagtgga agacaccgtg ccggaaaaag ctccggaaat cgaggcggca atgcgctcgt
    12901 cagtattcga ggccatggac aggtcactga gcgatgcgtt gtcccgtatc accctggagg
    12961 atctggtctt agaaacagaa aaatacagcg gtggacagtc gatcatgtat tatatatgaa
    13021 gtttatatat gatgaagtgt acgaggtaag agatatagat atattcagca ggaagtaatc
    13081 agcagcggac aggcaaagac ctggtgataa ggtataagac ctggaaagaa gataataggt
    13141 aaaggacgaa gataaacgat atgaaacatg agactcgaca tatgcaatat gatgcctggg
    13201 cctaaagata tgacgatatg tgaaaatctt gatccgagat atgagatttg ccgtaagagg
    13261 ggtatgttta tggaattcga aactttatgc atccacggaa acgatacaag atatgattcc
    13321 accggtgcga tcagcgtccc gatcttccag tctgccacct tcgcccaccc cggtgtcggg
    13381 ctgagcacgg gatacgatta ttccaggctg cagaacccga cgcgggaaca cctggaaaaa
    13441 accattgcga aactcgagaa cggcatcgat gccatggctt tttccacggg aatggcagcc
    13501 atcgcagcgc taatggagct cttcagcccc ggggaccatt tcattgcctc ggacgatctt
    13561 tacggaggtt cccaccggct gttctgccac atatccggaa aaaacgggct ttcattcgat
    13621 tttgccgata cctcggatat agaaaaggta tcctcgctta tcaggccgga gacaaaagcc
    13681 atctttgtcg aaacgcctac caacccgatg atgcaggtca cggacatcgc agccgtctca
    13741 gagctctgta aggagcacgg ccttctgctg gtcgtggaca acactttcct gacgccgtgt
    13801 ttccagaagc ctctggatct aggcgccgat atagtggtcc acagcggcac gaaatatctc
    13861 ggcggacata acgacacgct ggcaggattc cttgcagtgt cggatgcagc cctgtccgag
    13921 cggctgcgct tcatatacaa gacgacgggg gcgtgcctct cgccattcga cagctggctc
    13981 atgatccgcg gtgtcaaaac gctggctgtc cggatggaac gccagcagga aaacgcgatg
    14041 aagatcgccc aatggctctg cacccataaa aagatcaaaa aggtatatta tgtcggtctc
    14101 ccgtcccatc cccagtatga gatatcgaaa aagcaaacga caggtttcgg agcgatgatc
    14161 tcatttgaaa cagacagcga gaaaacagcc ctgggactcc tgcagcgggt gaatgtcata
    14221 cagtatgccg aaagtctcgg cggcgtggaa agcctcgtca catacccgat gctgcagacc
    14281 cacgccgaca taccaaagga agcgcgggag gccaagggta taaatgaacg tttgctgcgc
    14341 ctctctgttg ggctggaatc cgcggctgac ctgatcgcgg acctggagca ggcattgggt
    14401 gattaaccga tacagtttat gaagaaacag aactttccga aaacaagtgt tggatcacca
    14461 acttatactt cgaaccggag gcaaacaaga tatgacatac tcatttgata aagtaatcga
    14521 ccgtcacgga actgactcgc tgaagtatga tcttgcggca aaaagggggc tgccggaaga
    14581 tatcctcccg ttatgggtgg ccgacatgga tttcccttca ccgccggaag tattggaagc
    14641 gctcgttgat aaaagcctcc acggcatttt cggatacagc gacgccataa atgacgacta
    14701 ctacaacgca ttggccgatt ggtacgccag ccggttcggc tggcatcttt cacccaagtg
    14761 gcttgtaaaa acacccggcg tcgtttttgc tgtctgcact gcgatccgcg cactgacgaa
    14821 tgaaggcgac tctgtcctta tccagcagcc ggtctaccat ccgttcgcag gatctgtcct
    14881 cgcaaacgac cgcaaactgg tcgtaaatga actggtatat aatgacggta agtattcgat
    14941 agattttgac gatttcgaag acaaaatagt caaaaacaac gtgaagctct ttatcctctg
    15001 cagcccgcac aatccggtcg gacgcgtatg gacgaaggac gagcttgccg cgatgggaga
    15061 aatatgctta aaacacggca taaaggtagt tgcggatgag atacacgccg atttcgtcta
    15121 tcccggatac cgccaccacg ttttcgaaac tgtaaggccg gagttttccg atataacagt
    15181 tacctgcacg gctccgacca agacattcaa cctgg
//
LOCUS       DAOSCC010000027        14995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2716_length_14995_cov_4.850134, whole genome shotgun sequence.
ACCESSION   DAOSCC010000027 DAOSCC010000000
VERSION     DAOSCC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_14995_cov_4.850134"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..924
                     /locus_tag="PLG72_02765"
     CDS             211..924
                     /locus_tag="PLG72_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79755.1"
                     /translation="MIKVEHLVKKYDKVTAVNDISFTVHPGEVTVLLGPNGAGKSTAI
                     KSIAGLLNYKGEITICGMPNKSIEAKRVFGYVPEIPALFELMTIWDHVQFIAKAYRLE
                     EGWQEEAEQLLKRFEILDKKDSLSRELSKGMLQKVSIVLALMINPKAVLFDEPLIGLD
                     PKAISEMLAIFEELKRKGVSILISTHIIDTISDVWDRAYIMNKGSIVYETTREGTNGK
                     DLKSLFFEMTDVNDAEVSA"
     gene            921..2480
                     /locus_tag="PLG72_02770"
     CDS             921..2480
                     /locus_tag="PLG72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HOJ79756.1"
                     /translation="MNAVVYLLLRQYANRIRRLFRKPLNALLTIIAALFILSGPILML
                     VIPEFYEGLVGARGREIAVAGVQLFIGMTLILSSLSQQGGLFTYSEASILFSAPLTKR
                     TVLLYSTLQSLPGSILTSIFMCFYFPWLIGSAMTPLKLLATLLVMALLIFCISILYYY
                     IYIQDIAHEGLKKRLKKYLLIFFGAVAVIYAVIWLTNGHDFGEAAITFFTSPFYNAFP
                     LFGWAKWAVAALLNDQYITGFIPAILLLLVSAFVLARIYYSQDVDFYEKAQLDSIRLQ
                     KVVDALRSNGYDAAGLTEQKVRRTKGSFREGAAAIMSRQFLEMKKRGPMLAFRDLING
                     LIYIVIGLAAGFDFTIVFTMIVFSMIVNTTTDSWNAEFKKPYVYLIPESPFRKLMYAI
                     LPSIVKNMLGESIIVVIAAALFGAGVREAVFSVILLAAYTLLFTSAGVFTYRIMGHMT
                     NTVLLMFMRQLFIMAAAVPGGVIAIVLNLAAGIGDPVLLMIPVILANLLCSLLFMFLS
                     RKLLLQSELMN"
     gene            complement(2961..4514)
                     /locus_tag="PLG72_02775"
     CDS             complement(2961..4514)
                     /locus_tag="PLG72_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ79757.1"
                     /translation="MSNENSIKFSGVPASLWIGTTSDTNYASLEDDTDVDVAIVGAGI
                     TGLTTAYLLRGHGIRIAVIEANRIAKGVSAHTTAKITSQHALKYYKMINAVGEEKAKQ
                     YAQANQSAIDLIDKIIHDNNIDCDFKRVPAYVFTQDRNMIQKLEDETGSALRLGLPAE
                     YAETLDLPFGIEGAMKFTDQAYFHPRKYLLALAGLIEGEDCKIYENTRAVDIREGDPN
                     IVVTDNGRKIRARYVVQATRYPFFDKPGLYFARVYPQRTYLMALRIRDSFPEGMYINA
                     EEPTRTLRPHTGSDISAILVGGENHKTGHGKNLHNHYEIIRDFAKPMFDIESIPYRWS
                     AQDYTAMDEIPFIGPMTSGRETIFVATGFDKWGMTNGTAAAMIISDLIIKGESPWEEV
                     FSPSRFTPAASAKNFIKENFDVAVNLISGKLNIPEGTEKIEKGNAEITEADGYKVGVY
                     RDNDGKLHYVSTTCTHLGCELKWNSAELSWDCPCHGSRFTYTGEVIEGPALESIKPME
                     SDLCTGAGK"
     gene            complement(5033..6163)
                     /locus_tag="PLG72_02780"
     CDS             complement(5033..6163)
                     /locus_tag="PLG72_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HOJ79758.1"
                     /translation="MNETKTGFFKSFFYSITSFSKYRLFLRQSTGRVVAYLILLSLVL
                     SLATCASWFSQVSELIDIFSEEVLDDVPDFKLENGKLEIYAEMPIVIDGDLPIVIDTR
                     TDADPEDILYQYDNVILITSEKMIQKNYLQRQEVPWSIYGNATMTRDSLVAAIPILKP
                     TLVIVFVIIAIFFSVFFIAGKFFSAFIVSLIGRAVNAARGTRLSSRNIFKISVYSMTL
                     PLIVGTVLDIFRISIPFLWVLFYVGCSIYVYGALSSIKAALDTMYGNFGDFGNSGTFG
                     GFDDFGGFGGPGNGSDDGGESGNGNYDENDRGKDHDGTGNHDAQGDSPGTDDSTEPDK
                     QAGSDASAGTNEPLGSDHSPGPDDNTGPDDSSKSDDPAGSAE"
     gene            6530..6913
                     /locus_tag="PLG72_02785"
     CDS             6530..6913
                     /locus_tag="PLG72_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ79759.1"
                     /translation="MFIALSNNDPTPLYEQIKKQIIEQIMSGELPPGYMLPSIRALAK
                     ELEISVITVKKAYEDLEAGGYIVTNQGKGSFVAQAGAEFVRESKLKEIQKSFEEGIAL
                     CRELGFDDDGIRKVFGDILDEFGQV"
     gene            7058..7945
                     /locus_tag="PLG72_02790"
     CDS             7058..7945
                     /locus_tag="PLG72_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79760.1"
                     /translation="MDAVLNVDRLVKKYEKFSLKEVSFQIPRGYIMGFIGRNGAGKTT
                     VIKCIMDLIPFDAGSIEVFGLDSRRDSDEIRRRVGYVSEEQYFYENMTAGWNLDFFGG
                     FYPNWNRDLGLSLMKKFDIDPSKNVGELSKGMKVKLALALALAHKPEFLILDEPTSGL
                     DPVIRNELLELLLEVVQDENCSIFFSSHITSDIEKIADFVTFIEDGRIVISDEKDAIL
                     DKWRLFKVENSCGAAGLSERLIGVKKGEFGFSGMTDDPESFIRELTGNYPDVRYKVEK
                     VSLDDLLVRIVREEEENEE"
     gene            7935..8615
                     /locus_tag="PLG72_02795"
     CDS             7935..8615
                     /locus_tag="PLG72_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOJ79761.1"
                     /translation="MKNETIRLAVKDFQIQVKWIKSYVITLLVFLAMFLLMSPKQYQM
                     AFSMAALVTLYRFINLTMYEDEKNNTLRLIDSLPVGRETVVKARYLSTGILTAALIIV
                     LGAVSFIADSTDRLASAAATICVFLIYVILMAVYMPIGFKLGYIKAVNVNRFVFIGIF
                     LLIGIGSMLMGNLAGQRGYDAAGSLTSMFADTSPALVTAVFAVAAILLYIVSMKVSVV
                     FYKKRELF"
     gene            8624..9787
                     /locus_tag="PLG72_02800"
     CDS             8624..9787
                     /locus_tag="PLG72_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOJ79762.1"
                     /translation="MSKLQLIATSAFGIESVVARELKWLGYEDQQVENGKVTFAGDEE
                     AICRSNIWLRTADRVLVKMGEFEALTFDELFEGTKALPWDEWLPENAEFPVDGKSVSS
                     KLASVPDCQAIVKKAIVEKMKQRYKKQWFEENGPLYRIEVSLLKDRATLTIDTSGPGL
                     HKRGYRDLVGEAPLKETMASALLLISRWRPGRELIDPFCGSGTIPVEAAMMAANMAPG
                     AKRSFSAEKWRQAPAALWKRAREEAADKVKIDGEIRIHGSDIDDGVLSLARHHAEKAG
                     VSQLIHLQRRDFREISSKHKYGFIITNPPYGERIGELPEVERIYRDLGGIFKKFDTWS
                     FYVLTSHQRFEALVGRRADKKRKLYNGRIQCNYYQFFGPPPPKPAREEADPST"
     gene            10137..11456
                     /gene="aroA"
                     /locus_tag="PLG72_02805"
     CDS             10137..11456
                     /gene="aroA"
                     /locus_tag="PLG72_02805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ79763.1"
                     /translation="MLINVTRSRTRGTVRIPGSKSHTIRALFIASLAEGRSVISHPLL
                     SKDAFSAVEVCKAFGACIENTGDSFIIDGFGGVPAIPDDVVDVGNSGTTLRFAMMTAG
                     LAEGWTVFTGDSQIRRRPLGPLIKAMNQLGGNVFSTRGNDMAPVAVRGRLKGGSTDLD
                     AFTSQYLSSILINAPLLDQDTEVIITRLNERPYVEMTMWWLDGQGIEYANEDFRRIKI
                     KGRQRYRAFSTEIPGDFSSATFFMALAAISGEEFVLKNLDMTDPQGDKMVLRYLEDMG
                     AAVSCREDGIVIRGNGLKGIEIDMNATPDALPAMAVAGCFAKGETRLVNVPQARVKET
                     DRIRVMCSELSKMGADVEELPDGLIIRESKLRGCRVNGHGDHRVVMALAIAGLNAEGE
                     TCIETAEAVDVTFPEFPQLIRQCGGDIRMIRESSDETGDGAETGTAI"
     gene            11511..12692
                     /gene="aroC"
                     /locus_tag="PLG72_02810"
     CDS             11511..12692
                     /gene="aroC"
                     /locus_tag="PLG72_02810"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ79764.1"
                     /translation="MLGNTFGRMFRITTCGESYSGGFRKHLPVPPELYGGQIVIVDGV
                     PPGLRITAEVIEEELAKRRPGLSEFSTPRREKDRVFIFSGVMENDLTTGAPVGMIIPN
                     TDIGDVHLQQHKANRELVRPGQASYTYFRKYGRYYDWAGAGRASGRETAARVAGGAVA
                     KLILDAMGIDVIAYITEIHGIKAAPVTYETAKANYRRNEINCPDPEAAQKMIEELRNI
                     KAAGDSCGGVVEILARGVPAGLGEPVFDKLSAMIAHAMLSIGGVKGVEFGDGFEHARM
                     TGSESNDTPYFDEKTGRIRFRTNRAGGLLGGISNGEDIRVRVAVKPTPTILKSQETAD
                     IAKSENVTHVFASRSDPTICTRIYPVCEAMMRIVLVDAIYMAEGYRNVISAIDPEWEG
                     I"
     gene            12689..13195
                     /locus_tag="PLG72_02815"
     CDS             12689..13195
                     /locus_tag="PLG72_02815"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ79765.1"
                     /translation="MTPDTKGYGNMILIGMPGSGKSTVGPLLARKTGKSFADTDDIIR
                     KTDGRELGEIVRTEGHIRFLELQERIITSMDFRDHVIATGGGVVKSDGLMQFFRQIGT
                     VIFLDEDPATLEKRLAPGRRLARPEGQTFKAVYEERKPLYTKYADRIIDCRGKSADEI
                     VMEILSDE"
     gene            13188..14006
                     /gene="spo0A"
                     /locus_tag="PLG72_02820"
     CDS             13188..14006
                     /gene="spo0A"
                     /locus_tag="PLG72_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOJ79766.1"
                     /translation="MSDPVSVLIVDDNKQLCELLTESINSTGDMRVAGTASDGKTAMD
                     MIRELEPDVVLLDIIIPNLDGLAVLEGIAGRKDGKRPAIIVSTSVGSESIIKRAMELG
                     ADYYILKPFEISVLITRIRQICLEEPAYGRVIARSDVEAQPYGGDAARVVTELIRSIG
                     VTPNLSGFNYLREAVLLGMEQPARLENVSRNIYTILADRHGTKARNIDRAIRCALTSA
                     QNKTRNSSNLMNIMQDSILFNNGRRPNNSQVIAFLAKKAGRRLLEARSGDCNMV"
     gene            complement(14106..14387)
                     /locus_tag="PLG72_02825"
     CDS             complement(14106..14387)
                     /locus_tag="PLG72_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ79767.1"
                     /translation="MNKTDLINSIASKSGLNKKNSEAALNAFISSVEEALVKGDKVVL
                     VGFGTFDVKQRAARKGRNPQTKKEIQIPASKAPVFKAGKVLKEKVNKKK"
     gene            complement(14515..14886)
                     /locus_tag="PLG72_02830"
     CDS             complement(14515..14886)
                     /locus_tag="PLG72_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ79768.1"
                     /translation="MNILVCVTQQKTCERLINQAHELAREYGGNLFVINVVRSDMSFL
                     DSSRESEALEYLFGISKKVGANLAVLKSDDVVKTIAEYADENDIDYIVIGKSPDSNAG
                     ERFVKKLKSLLKNDAEIRILS"
BASE COUNT         3856 a         3418 c         4071 g         3650 t
ORIGIN      
        1 gaactgggtt tagcctcata aacaattttt ggatttattt ttgctcattt atagattttc
       61 tttgaactat gtgttataat attccataaa atttctggca tactgtgcac atcagaccag
      121 ttatgggata tgcgcgtaaa ctatatacgg atcatacaca acagcttctt tccggtttat
      181 agcaggaagc ggatcatgag gaggtcagaa gtgatcaagg tagaacatct tgtaaaaaag
      241 tatgacaaag ttacagcggt aaacgacata agtttcaccg tccaccccgg tgaagtgacc
      301 gtgctgcttg gccccaacgg cgcgggcaaa tcgacggcca tcaagagtat cgcaggtctg
      361 cttaattata aaggcgagat cacaatttgc ggaatgccta ataaaagtat cgaggcaaaa
      421 agggttttcg gttacgtgcc ggagatcccg gcgctgttcg aactgatgac gatatgggac
      481 catgtgcagt tcatagcaaa ggcatacagg ctagaagaag gatggcagga ggaagcggaa
      541 cagctgctga aaagattcga gatactggat aaaaaagatt cgttgagcag ggaactgtct
      601 aaaggcatgc tccagaaagt cagtatcgtt ctggctctga tgataaatcc aaaagccgtg
      661 ctgttcgatg agccgctgat agggcttgat ccaaaggcta tcagcgaaat gctggccatt
      721 ttcgaagagc ttaagcgcaa gggggtcagc atcctgataa gcacgcacat catcgacact
      781 atcagtgatg tttgggacag ggcttatatc atgaacaagg gatccatcgt atatgagact
      841 accagggaag gaacgaacgg aaaggatctc aagtccctct tctttgaaat gactgatgtg
      901 aacgatgcgg aggtatcagc atgaacgcgg tggtttattt gctgctccgg cagtatgcca
      961 acaggatcag gcggctgttc agaaaaccgc tgaatgcctt attgacgata atagcggccc
     1021 tgttcatatt gagcggacct atattgatgc ttgtgatccc tgagttttat gaaggcttag
     1081 tcggcgccag gggaagagag atcgctgtcg caggagtaca gctttttatc ggaatgacac
     1141 tgattttgtc ttcactgtcg cagcagggcg ggcttttcac atactcggaa gcgagcattt
     1201 tgttcagcgc gccgctgacc aagaggacgg tacttctgta ttccacgctg cagtcgctgc
     1261 ccggctcgat cctgacgtcg atattcatgt gtttctattt cccatggctc atcggaagcg
     1321 cgatgactcc gctgaaactg ctggcaacgc ttttggtgat ggcgctgctg atcttctgta
     1381 tatccatact ctactattat atctatatcc aggatatcgc ccatgaaggc ctgaaaaaga
     1441 gactgaagaa gtatttgctg atattcttcg gagcggtcgc agttatatat gcagtgatct
     1501 ggctgacgaa cggacatgat ttcggggagg ccgcaataac atttttcacc agtccgttct
     1561 acaatgcatt cccgctgttc ggctgggcaa aatgggcggt tgcggctttg ctgaatgatc
     1621 agtatattac aggtttcata cctgcgatac tgcttctgct cgtttcagca tttgtacttg
     1681 ccaggatata ctattcgcag gatgtggact tctacgaaaa ggcgcagctt gactccatca
     1741 ggctgcagaa ggtggtggac gccctcagga gcaacgggta cgatgcagcc ggcctgactg
     1801 aacaaaaagt ccgccggacc aaaggcagtt tcagggaagg ggcagcggcc ataatgtcga
     1861 ggcagttcct tgagatgaaa aaaagaggcc cgatgctggc gttcagggat ctgatcaacg
     1921 gtttgatcta tattgtgatc ggactggcgg ccgggttcga ttttacgatc gtgttcacaa
     1981 tgatcgtttt ttcgatgatc gtaaacacga ctacggacag ttggaatgcg gaattcaaga
     2041 aaccatacgt atatcttata cctgaaagtc cgttcagaaa gctgatgtat gcgatactgc
     2101 ccagcatcgt taagaacatg ctgggagaat cgatcatagt ggtgatagcg gcggccctgt
     2161 tcggagcagg agtacgggag gctgtgttca gtgtgatcct gcttgcggca tatacgctgc
     2221 tttttacttc agcaggcgtt tttacataca ggatcatggg acatatgacg aatacggtat
     2281 tgctgatgtt catgaggcag ttgttcataa tggcggcagc ggtaccgggc ggtgttatag
     2341 ccatagtcct gaacctggca gcaggtatcg gagacccggt gctgctgatg atacccgtca
     2401 tcctggccaa cctgctttgc tcgctgctct tcatgttcct gagccggaaa ctgcttttac
     2461 agagcgagct aatgaattga agcttatgga ttggtacgga tgcctgctgg aatggacagc
     2521 gggcattttt actgtgtgct gcctgatttg attacatgca ggaaaagccg gcggatgccc
     2581 ggtcagaaag ctcgcccgta aagcacagat atctggggct attttatgca tcaagtatgg
     2641 tagaagccgg cggatgcccg atcaagatat ttatgagttg caagtgatac agatacggcc
     2701 gggtcatgca gcccgaatga ggcaacagga accacaaccg gcagcatgga cgctgccggc
     2761 gctggtcccg agccacggac ggcgagtgac ctgcgccctc attggagcaa atgagccggt
     2821 cagcagctgt tcacgaagca actccaatgc atgatcgggc atccgccggc ttctgcatca
     2881 gtgtatgacg gggtagccgc actgagtcgg taagttgccg actgcctggc acagatccgg
     2941 atgcgattgc cggacgccgg ctattttcct gcgcctgtac agaggtcgct ttccataggc
     3001 tttatcgatt caagcgcagg gccttcaatg acctcgccgg tgtatgtgaa tcttgatccg
     3061 tggcacgggc agtcccacga cagttctgcc gaattccact tgagttcgca gcccaggtgg
     3121 gtgcaggtcg tgctcacata gtggagtttg ccgtcgttgt cacggtaaac gccgacctta
     3181 tagccgtccg cttctgtgat ctccgcattt cctttctcga ttttttcggt cccttcgggg
     3241 atgttcagct tgccggatat caggtttacc gcaacatcga agttttcttt tatgaagttc
     3301 ttggccgatg ccgcgggcgt gaagcgcgaa ggactgaaca cttcttccca cgggctttct
     3361 ccctttatga tcaggtcgga gatgatcatt gccgctgcgg taccgttcgt catgccccat
     3421 ttgtcaaagc cggtggctac gaatatcgtt tccctgcctg acgtcatggg acctatgaac
     3481 ggtatctcgt ccattgcggt ataatcctgg gccgaccatc tgtatggtat gctttcaata
     3541 tcgaacatcg gctttgcgaa atccctgatg atctcgtagt ggttgtgcag gtttttgcca
     3601 tgccctgtct tgtggttctc gcctcccacc agtatcgctg aaatatcgct gcctgtatgc
     3661 ggacgaagag tcctcgtcgg ttcttctgca ttgatataca tgccttcagg gaaagagtcc
     3721 cttatccgga gcgccatcag gtatgtgcgc tgagggtaga ctctggcaaa ataaagtcca
     3781 ggcttgtcaa agaaaggata ccgtgtggcc tgtaccacgt atcttgccct gattttcctt
     3841 ccgttgtcag taacgactat attggggtcg ccttcccgga tatcgacagc ccgggtgttt
     3901 tcatatattt tgcagtcctc gccctcgatc agcccggcca atgccagcag atatttccgg
     3961 ggatggaaat atgcctggtc tgtgaatttc atcgctccct cgatgccgaa gggaagatcg
     4021 agcgtttccg catattcggc gggcagtcca agcctgagtg ccgatcctgt ttcatcttca
     4081 agcttctgta tcatgttcct gtcctgggtg aacacatatg cagggaccct tttgaagtca
     4141 cagtctatat tgttgtcatg gattatttta tctatcaggt cgatggcaga ctggtttgcc
     4201 tgggcatact gctttgcctt ttcttctccg acggcattga tcattttgta gtatttgagt
     4261 gcatgctgcg acgtgatctt ggctgtcgta tgcgcagata cgccttttgc aatgcggttg
     4321 gcctcgataa cggctatcct gatgccgtgc ccgcgaagaa gatatgcagt cgtaagtccg
     4381 gtgatacccg cgcccacgat cgccacatcc acatccgtgt catcttccag ggatgcgtaa
     4441 tttgtgtcgc ttgtggtacc gatccagagc gatgccggta cgccgctgaa ctttatactg
     4501 ttttcattgc tcatattcat gtgatcctcc aaaaaaaatg catgatagat cgtatgtaat
     4561 cagttgttca ctatgcagct gcgcggtttg gcggcatgtg ccgcagacag tgcagtgcgg
     4621 catacgcgca aagttgctca gcaacatacg ccggcattta tatatattct tcaaaagtcg
     4681 gattttcatg caatatagtg cgatataagc aggcaggaat caagtgatga aataagcggc
     4741 cggtaaaatg ccgaaccggg tgtcgcccgg acatgcctgc cgatttttgc attcggttgg
     4801 caaaatcacg ccggtaactt gttggaaaaa ccgatcagta atttcagtac aggataacag
     4861 ggtggtgcgg tacggttcaa aaggcggtgc ataatatgcg taataaaaga gactgcacca
     4921 cagggccgga ccctcagtgc agtctttctc cggatcacat gattcgcctt gccgtgaccg
     4981 gaccaggccg attatcagtt ccgacataat gatccatttg cttttggtgc gttcattcag
     5041 ccgatccggc aggatcatcc gatttggaag agtcatcagg acccgtgtta tcatccggcc
     5101 ctggagaatg atctgatccc aaaggctcgt tcgttccggc agaagcatcc gatcctgcct
     5161 gtttatccgg ttctgtggag tcatccgtcc cggggctgtc gccctgggcg tcgtgatttc
     5221 ctgtgccgtc atgatctttc ccacggtcat tttcgtcata attgccattg ccggattcgc
     5281 cgccgtcgtc cgatccgttg cctggaccgc caaagccgcc gaaatcgtca aaacctccga
     5341 aagtaccgga gttgccgaaa tcgccgaagt tcccgtacat cgtatcaagt gcggctttta
     5401 tgctgcttaa ggccccgtag acgtatatgc tgcagcctac gtagaacaat acccacagga
     5461 aaggtatgct gatcctgaat atatccagta cggtaccgac tatcaggggc aatgtcatgg
     5521 aatatacact gatcttgaat atgttgcggc ttgaaagccg tgtccccctg gctgcattga
     5581 cagcccggcc gatcaggctg acgatgaatg cgctgaagaa tttccccgct atgaaaaaca
     5641 cgctgaagaa aatggcaatt ataacgaaaa cgatcacaag tgtcggtttc agtatcggga
     5701 tcgccgctac aaggctgtcc cgtgtcatag ttgcattgcc gtatatactc cacggcactt
     5761 cctgccgctg cagataattc ttctggatca tcttttcgct ggtgatcagt attacattat
     5821 cgtactggta caggatatct tccgggtctg catcggtcct ggtatcgatc acgatgggaa
     5881 gatcaccgtc gatgacgata ggcatttcag catatatctc cagtttgccg ttttcgagct
     5941 tgaaatccgg cacgtcatcg agtacttctt ccgaaaatat gtctatcaac tcgcttacct
     6001 gggagaacca acttgcacat gtggccaatg aaagcacaag agacagcagt atcagatagg
     6061 cgacgaccct gccggtgctt tgcctcaaaa acagcctgta tttgctgaag cttgtgatac
     6121 tgtaaaaaaa gctcttaaag aatcctgttt tcgtttcgtt cataagaacc tccggatatt
     6181 tgttatactc ggcattatcg attattggca ttatagataa ccggtattat cgcatttaca
     6241 caaacgcccg tttccggtgc tgtactcggg aaataaactt cggacaggcg catggtattt
     6301 ctccgctcgc gatccgtgcg gtcatcagcc tgttttatcc cgagtctgcg gctgtatgag
     6361 cggcacgtgg aagtgctgcc gataaccata cttattatac aataagggga tattttttca
     6421 aatttaatta ttgtcgaaat attgacagtg tatatacaca gtgataaact gtatatgaac
     6481 tgtatatata tagacataca cagttgagaa ttccgcaaga ggtggttttt tgttcatagc
     6541 actgtcgaat aatgatccga ctccgctgta tgagcaaatc aagaagcaga taatagagca
     6601 gatcatgagc ggggaactgc cgccgggata tatgctgccg tcgatcaggg ctcttgccaa
     6661 ggaacttgag ataagcgtga tcacggtgaa aaaggcatat gaagaccttg aggccggcgg
     6721 atatatagtg acaaaccagg ggaaaggctc atttgtcgcc caggccgggg cagagttcgt
     6781 cagggagtcc aaactgaaag agatacaaaa aagctttgaa gaggggatcg cactgtgcag
     6841 ggagcttggc tttgacgacg acggcatccg aaaggtattc ggggatatac ttgatgagtt
     6901 cggacaggtg tgacccgcag ccatggcacg gacgcatgca gagcatgaat atgttcagat
     6961 catgcatcaa gccatgcact tgctcaaagc gcagatatat gcaaagttcg gtcatgtcca
     7021 aacgatcatt ttatataaac agattggagt ggtttgaatg gatgctgtac tgaatgtgga
     7081 cagacttgtc aaaaagtacg agaagttttc attgaaagaa gtgagctttc agatccctag
     7141 agggtacatc atgggattca tcggcaggaa cggagccggc aagactactg tgatcaaatg
     7201 cataatggac ctgatacctt ttgatgcagg ttcaatcgaa gtattcggcc ttgacagcag
     7261 gcgggactcc gatgagatca ggagaagagt aggttatgtg agtgaagagc agtactttta
     7321 tgaaaacatg acggcaggct ggaacctgga tttctttggc ggcttttatc cgaactggaa
     7381 cagggacctg ggtctgtcgc tgatgaaaaa attcgatatc gacccatcaa aaaacgtcgg
     7441 tgaactgtca aagggcatga aagtcaaact ggcccttgca cttgcactgg cacacaagcc
     7501 ggaattcctc atacttgacg agcctacttc cgggcttgat cctgtcatca ggaacgaatt
     7561 gctggaactc ctgctggaag tggtacagga tgagaactgc tccatattct tttcttcgca
     7621 tataacttca gatatcgaaa aaatagctga tttcgtaaca ttcatcgaag acggcaggat
     7681 agtgatcagc gatgagaaag atgcgatatt ggacaaatgg aggctgttca aggtcgagaa
     7741 cagttgcggc gccgccggcc tgtcagagcg gctgatcggc gtcaaaaaag gcgaattcgg
     7801 tttcagcggc atgacagacg atccggaaag ctttatcagg gaattgaccg gtaattatcc
     7861 ggacgtccgt tataaagtcg aaaaggtatc gctggatgac cttctggtca ggatagtaag
     7921 ggaggaagag gaaaatgaag aatgaaacga tccgccttgc ggtaaaggat tttcagatac
     7981 aggtaaagtg gataaaaagt tacgttatca cgctgctcgt gtttcttgcc atgtttcttc
     8041 taatgagccc aaagcaatac cagatggcgt tttcaatggc tgccctggtc acgttgtacc
     8101 gctttatcaa tttgactatg tatgaggacg agaaaaacaa cacgctgaga ttgatcgact
     8161 ccctgccggt cggacgggag actgtcgtaa aggccaggta cctcagcacc ggcatcctta
     8221 cggccgcact gatcatagtt cttggcgccg tatcgttcat tgctgacagc actgacagat
     8281 tggcttcagc ggcagcgacc atatgcgtat tcctgatcta tgtgatactg atggcagtgt
     8341 atatgcctat aggattcaaa ctgggatata taaaggcagt gaatgtcaac aggttcgttt
     8401 ttatcgggat tttcctgctg atcgggattg gttcgatgct gatgggcaac ctggccggac
     8461 agcgtggtta cgatgcggca gggagtttaa catcgatgtt cgccgacacg agccccgcac
     8521 tggtaacggc agttttcgca gtggctgcga ttttgctgta tattgtatca atgaaggtat
     8581 cggtggtatt ctataaaaaa cgggaactgt tttaggagga aacatgtcaa aacttcaact
     8641 gatcgccacg tcggcgttcg gcatagaatc ggtcgtagca agggaactga aatggctggg
     8701 atatgaagac cagcaggtcg aaaacgggaa agtcactttt gccggtgacg aagaggcaat
     8761 ctgcagatcg aacatatggc tgcgtactgc cgaccgtgtg ctggtgaaaa tgggcgaatt
     8821 cgaagccctt acctttgacg aactcttcga ggggacgaaa gccctcccgt gggacgagtg
     8881 gctccctgaa aatgcggaat tcccggtgga cggcaagtcg gtgagttcga agcttgccag
     8941 tgtgccggac tgccaggcca tcgtcaagaa ggccatcgtg gaaaagatga agcagcgcta
     9001 taaaaagcag tggttcgagg aaaacggacc gctgtacagg atagaggtga gccttctcaa
     9061 ggacagggcg acccttacca tagatacatc aggtccgggg ctccacaagc gcggttacag
     9121 ggatctcgtc ggcgaagccc cgctgaagga aacgatggct tccgccctgc tgcttataag
     9181 caggtggagg ccgggcagag aactgatcga tccgttctgc ggttcaggta cgatcccggt
     9241 cgaggcagcg atgatggcgg ccaacatggc gccgggagcg aaacgcagtt tttcagccga
     9301 gaaatggcgc caggcacctg ccgcactgtg gaaacgggcg agggaagaag cagcggacaa
     9361 ggtaaagatc gacggtgaga tcaggataca cggctccgat attgacgacg gggtactcag
     9421 ccttgccagg caccatgccg aaaaagccgg tgtttcacag cttatacacc ttcagagaag
     9481 agatttcagg gagataagtt caaagcacaa atatgggttt atcataacca accctccgta
     9541 tggcgagcgg ataggcgaac tgcccgaggt ggagcggata tacagggacc tgggcggtat
     9601 tttcaaaaaa ttcgatacat ggtctttcta cgtgcttaca tcccatcaga ggttcgaggc
     9661 gctggtcgga agaagggctg acaagaagcg gaaactgtat aacggcagga tccagtgcaa
     9721 ctactaccag tttttcggac cgccgccgcc aaagccagcc agggaggaag cagacccttc
     9781 gacctaatca ttcgatatcc ggctttgtac cgatatcagc aactcaatca ttcaataagc
     9841 agatttgtgc cgtttttcag cttttgggca catggatgtg catttattca acttttggga
     9901 cagcaaaatg atacataatc agtataaacc taatattatt tttactgttc tgatataaaa
     9961 gatgaatgat cgcagcatga atggagcacg ttctcgaatg atgataagaa aagagcggta
    10021 tgcagatgtg atcccggcca cggaacatgt atttgacgta atagctgcca tatattaata
    10081 tataaataac aaataacaga tatatggcag tttttgcaga acggtaggtt gggaaaatgc
    10141 tgataaacgt tacaaggtcc agaacccgcg gtaccgtcag gatacccggg tccaaatcac
    10201 ataccatcag ggcgcttttt atcgccagcc ttgcagaagg cagatccgtt atttcacatc
    10261 cccttttatc gaaagatgca ttttcggcag tcgaagtatg caaagctttt ggagcctgca
    10321 ttgaaaatac gggcgacagt ttcatcatcg acgggttcgg cggcgttccg gcgatcccgg
    10381 atgatgtggt ggatgtcggc aattcgggta caacgctgcg ctttgcgatg atgaccgcag
    10441 gactggctga aggatggact gttttcaccg gcgacagcca gatacgccgc aggcctctcg
    10501 ggcccctgat aaaggcgatg aaccagcttg gcggcaatgt gttttccaca cggggcaacg
    10561 acatggcgcc ggtagctgtg cgagggaggc tgaagggcgg aagcacggat ctggacgcct
    10621 tcacttcaca gtatctttct tcgatactga tcaatgcccc tctcctggac caggataccg
    10681 aggtcatcat tacaaggctc aatgaaaggc cgtatgtgga gatgaccatg tggtggctgg
    10741 acggccaggg aatcgaatat gccaacgaag atttcaggag aataaagata aaaggaaggc
    10801 agaggtacag ggctttcagc acggaaatcc ccggcgattt ctcatcggct actttcttta
    10861 tggcgctggc cgccatatca ggagaggaat tcgtactgaa gaacctcgat atgacggacc
    10921 cgcagggcga caaaatggtg ctgcgctacc tggaagacat gggtgctgcg gtcagttgcc
    10981 gcgaggatgg gatagtgatc aggggaaacg ggctgaaagg cattgagatc gatatgaacg
    11041 cgacacctga cgccctccct gcgatggctg tcgcgggatg tttcgcgaag ggcgagacaa
    11101 ggctggtcaa tgtgccgcag gcaagggtga aggagactga ccggatccgg gtgatgtgct
    11161 ccgagctttc gaagatgggc gccgatgtcg aggaactgcc tgacgggctt atcatacgtg
    11221 aaagcaaact caggggatgc agggtgaacg ggcacggcga ccacagggtc gtcatggcgc
    11281 tcgcgatagc ggggctgaac gcggaaggcg agacctgcat cgagacggca gaggcagttg
    11341 acgtcacgtt ccctgaattc ccgcagctta taaggcagtg cggcggtgac ataaggatga
    11401 tcagggagag ttcggatgaa accggcgacg gtgccgaaac cggaactgca atttgaatgg
    11461 atcagggcag atcgaaacaa gctggaacaa tacaggatcg gaggtaagag atgctgggaa
    11521 acacattcgg cagaatgttc aggataacta catgcggcga atcatattca ggaggtttca
    11581 ggaagcactt gcccgtgcct cccgaattgt acggcgggca gatagtgatc gtcgacggtg
    11641 tcccgccagg actcaggatt acggcggagg tcatcgagga ggagttagcc aagcggaggc
    11701 ccgggctttc ggaattcagt acgccgcgaa gggagaagga cagggttttc atattctccg
    11761 gggttatgga gaatgacctg accacaggag cccctgtagg gatgatcata cccaacaccg
    11821 acatcgggga cgtgcatctg cagcagcaca aggccaacag ggaactggtc aggccagggc
    11881 aggcatccta cacctatttc aggaaatacg gcaggtatta tgactgggcg ggcgcaggac
    11941 gggcatccgg acgtgaaact gccgccagag tggcgggagg cgctgtcgcc aagctcatcc
    12001 ttgatgcaat gggcatagat gtgatagctt acataaccga gatccacggg atcaaggctg
    12061 cgcctgtcac atatgagacg gctaaggcca attaccgcag gaatgagata aactgccctg
    12121 atccggaagc cgcgcagaaa atgatcgaag agttgagaaa tatcaaagct gccggggatt
    12181 catgcggcgg cgtggtcgag atccttgcaa ggggtgtgcc ggcagggctg ggagagcctg
    12241 tgttcgacaa gctcagcgcg atgatagccc atgccatgtt atccatagga ggagtcaaag
    12301 gcgtagagtt cggtgatgga ttcgaacatg cgaggatgac cggttctgag tcaaatgaca
    12361 cgccttattt cgacgagaaa accgggagga tccgcttcag gaccaatcgg gccggcggcc
    12421 tgcttggcgg tatctccaac ggtgaagata taagggtcag ggtggctgtg aagcccacgc
    12481 ctacaatttt gaaaagccag gagacggcag atatcgcaaa gtcggaaaac gttacccatg
    12541 tttttgcttc ccggagcgat ccgacgatct gtaccaggat atatccggtc tgtgaggcaa
    12601 tgatgaggat cgtcctggtg gatgcgatat atatggccga agggtacagg aatgtcatat
    12661 ctgccataga tcctgaatgg gagggtatat gacgccagat acaaaaggat acggaaatat
    12721 gatcctgata ggcatgcccg gttcggggaa aagcacggtt gggcctttgc ttgcccggaa
    12781 aaccgggaag agttttgcgg ataccgatga tataatacgc aaaaccgacg gaagggagtt
    12841 gggcgagatc gtaaggaccg aagggcatat aaggttcctt gaactgcagg agcggataat
    12901 cacatcgatg gatttccggg accacgtgat cgcaacaggc ggcggtgtgg tcaaaagtga
    12961 cggtctcatg cagttcttca ggcagatcgg gacggtcata ttccttgatg aggatcccgc
    13021 aacacttgag aaacggcttg ccccgggaag aaggcttgca aggccggaag gccagacatt
    13081 caaggctgtc tacgaggaac gaaaacctct ttatactaaa tatgcagaca gaataatcga
    13141 ctgcagaggc aaatctgcag acgaaatcgt aatggagata ttaagcgatg agtgaccctg
    13201 tttctgtttt gatcgtagac gacaacaagc aactgtgcga actgctgaca gaaagcatca
    13261 acagtaccgg agacatgcgc gtggcaggga cagccagtga tgggaaaacg gcaatggaca
    13321 tgatacggga actggaacct gatgtagtac tgctggacat cattataccg aacctggacg
    13381 gacttgccgt cctggaaggc atcgccggcc ggaaagacgg gaaaaggccc gccataatcg
    13441 tgtcaacctc ggtcggaagt gaaagcatca ttaagcgggc gatggaactg ggcgcagatt
    13501 attacatatt gaaacctttc gaaatcagtg tactgataac ccggataagg cagatctgtt
    13561 tagaggaacc ggcatatggc cgggtgatcg ccaggagcga tgttgaggcg cagccgtacg
    13621 gaggcgatgc tgcccgggta gttacggaac tgatcaggag cattggagtg actcccaacc
    13681 tgtcaggctt caattacctg agggaggcag tcttgctggg gatggagcag cctgccaggc
    13741 tagagaacgt gtcgaggaac atatatacca tacttgcgga tagacatggc acaaaagcgc
    13801 gaaatatcga cagagccata agatgcgccc tgacaagcgc gcaaaataag acaagaaatt
    13861 ccagtaacct gatgaatatc atgcaggatt cgattttatt caacaacgga aggcggccga
    13921 ataattccca ggtcatcgcc tttttggcga aaaaagcggg caggcggctt ttggaagcca
    13981 ggtccggcga ttgtaacatg gtgtgactat atagcataat ataggtcaac gataccaggc
    14041 atttcaaaac tgcggagaaa agctaaaaag ctgcctaaac ggcagctttt tatttattgg
    14101 tccgattatt ttttcttgtt tactttctcc tttaatactt tgccggcctt gaatactgga
    14161 gcctttgaag cggggatctg tatctctttc ttggtctgag gatttctgcc ttttcttgct
    14221 gctctctgtt ttacgtcaaa tgtaccaaaa ccaacaagaa caaccttgtc gccctttacg
    14281 agagcttcct caacggagct gatgaatgcg ttgagagctg cttcgctgtt tttcttgttc
    14341 agacctgatt ttgaggcaat agagttgatt aaatctgttt tgttcatcaa tacaacctcc
    14401 taaatttgga attcgacatt attttataac gaaaagcgcg tgaagtcaag catttcacga
    14461 taaattagta atttggtact acataaaata tgtaaaacag actaaatttt tttattacga
    14521 caaaattctt atttcagcat catttttcag caggcttttc agtttcttta cgaagcgttc
    14581 acccgcattc gaatcgggcg acttgccgat cacgatatag tcgatatcgt tctcatccgc
    14641 gtattcggct atggttttca ctacatcgtc agatttcagg acggcaaggt ttgccccgac
    14701 cttttttgaa atgccgaaca gatactccag cgcttcggat tccctggaac tgtccaggaa
    14761 gctcatatcg ctcctgacca catttatgac gaacagattg cctccgtatt cccttgcaag
    14821 ctcatgtgcc tggtttatca ggcgttcaca ggtcttttgc tgtgtaacgc aaacaagtat
    14881 gttcatggta cctccggtgg tgttttttcc gtagtatatt taccacatcc attatactta
    14941 taactataaa tgagcaaaag cgctaacata taatgtaaaa ttaaaggaga cacct
//
LOCUS       DAOSCC010000028        14961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2723_length_14961_cov_4.974037, whole genome shotgun sequence.
ACCESSION   DAOSCC010000028 DAOSCC010000000
VERSION     DAOSCC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_14961_cov_4.974037"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..854
                     /locus_tag="PLG72_02835"
                     /pseudo
     CDS             42..854
                     /locus_tag="PLG72_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            938..1501
                     /locus_tag="PLG72_02840"
     CDS             938..1501
                     /locus_tag="PLG72_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOJ79769.1"
                     /translation="MDNVKSINTPLTSDMARQLKAGDHVRISGVVYAARDAAHKRFIE
                     MLDAGKELPFDIKDQVIYYVGPCPARPGHVIGSAGPTTSGRMDAYAPRLIELGLTGMI
                     GKGLRSGEVVEAMKEYGAVYFGAVGGAGALLSKCIISDEIAAFPELGPEALRRLVVRN
                     FPAVVVIDSEGRDLYSTGRKKFQRVFV"
     gene            1608..2378
                     /gene="nadE"
                     /locus_tag="PLG72_02845"
     CDS             1608..2378
                     /gene="nadE"
                     /locus_tag="PLG72_02845"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ79770.1"
                     /translation="MIGNESIIKQFGVAKEYDVEADIKRRVDFLAKYLDANPWAEGYL
                     IGISGGIDSAVVAALLKMSVLDTNKRVLAVLNPYHSKNIDYALEVVHALDLEYRIRDI
                     GEIVDLAVRSFNKNASKLQIGNRMARQRMIEWYDIAAEERLIVAGTDHATESVIGYFT
                     KYGDGGVDINPIRTLDKRQIIQLAKYEWPFGKIPESVISRAPSADLWDGQTDEGEIGM
                     TYEVICDYLEGKDVDKEFAEKIEQMYHKSMHKRQIPFI"
     gene            2522..2731
                     /locus_tag="PLG72_02850"
     CDS             2522..2731
                     /locus_tag="PLG72_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOJ79771.1"
                     /translation="MAKVTKDMIISDILALDRGTAPILLKIGMHCLGCPASAGESLEE
                     ACAVHGVDADMIVKELNDYLESKQG"
     gene            2887..2962
                     /locus_tag="PLG72_02855"
     tRNA            2887..2962
                     /locus_tag="PLG72_02855"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Lys,seq:ttt)
     gene            3163..3519
                     /locus_tag="PLG72_02860"
     CDS             3163..3519
                     /locus_tag="PLG72_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79772.1"
                     /translation="MEHNLKIVLPYEGVKKYISAVRAAESPDHGRLWLEYAFDPYWNE
                     WAAGQFTEERTRSEMSDPVTDIDELETAADLLIASDTEGLLRNTYDKINTTVEAQISS
                     DTAGRFSCLACAARQA"
     gene            complement(3551..4837)
                     /locus_tag="PLG72_02865"
     CDS             complement(3551..4837)
                     /locus_tag="PLG72_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOJ79773.1"
                     /translation="MNNVKKLSDFIDSLNDEKKPTVKADSEELNRMFDAVRQIKSLKS
                     PAMPDEDFPHRIIQNLTEKNRHTAKSGKRTWAGGFAGIAAVLAVALLANFAGPIKNSY
                     VVYAVEQAYNDVKAYHGTLEVVFSNEAGETQTQSVLDVWADKSGNYYIEVLEGSYKGL
                     KTVSDGKNVWQSPTDENAMNVLPVFSETNEFIFELGNEVDELQNAESARIIGDDDIAG
                     RPAYVMEVTPKGGLPYKLWVDKGTKIPLQKQSSMYKAIQYTTRYTVINFAESIPEELL
                     TVGPDTGFKEVDPDGYKNTVKNPDKPEDITADIAENNKADSDTAKYTPEITVDVDMDI
                     EKAEQIAADSGSSPWKLDPVYVAQVFISLQISPEGIVGEYPIHYNEFVIAESNGTSAK
                     IEVISEKTHIKAVYLEKLIRQDDTGIWTVTGYDISP"
     gene            complement(4830..5348)
                     /locus_tag="PLG72_02870"
     CDS             complement(4830..5348)
                     /locus_tag="PLG72_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ79774.1"
                     /translation="MAEDNLRSIEDICEQTWEPLYRFIYYKVQNREEAEDITQDTYVR
                     SLSWPGFDRIRAGRYMAFFKTVAMNIIRDRWRKKKTRGTSIDIDSIAQTKVLSDDITD
                     IANRHLLIRRSLEKLNEDQRKVIELRIIEGYSVSETAGLMGIKAGNVRVLQYRALKNL
                     VIIMNEEENHHE"
     gene            5529..5879
                     /locus_tag="PLG72_02875"
     CDS             5529..5879
                     /locus_tag="PLG72_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOJ79775.1"
                     /translation="MTAERVYKMPFSKVYPLLVQKAERKGRTRSEVDTVICWLTGYDE
                     SGLQEQIAKNVDYETFFNEAPLINPNAVKITGVICGHRVEEIRDPLMQKIRWLDKLVD
                     ELAKGKPMEKILRS"
     gene            6042..6614
                     /locus_tag="PLG72_02880"
     CDS             6042..6614
                     /locus_tag="PLG72_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ79776.1"
                     /translation="MPDMERMDKHLLDLGGTYMLNHARRCLVIAKKLAEEENLAYDED
                     ILAFSCYFHDISAYKPYRPDGPFDHAEESAKIVPAIAEEYGINDADKIRIIVEAVKYH
                     DKRGQGTANETILVRNADAVDYLGFVAVARDFSKQPNEMTKAVAALKKHLAGFSALLE
                     LDSARRMAGPRIKKLETFLHEFEQETFGLY"
     gene            6864..7157
                     /locus_tag="PLG72_02885"
     CDS             6864..7157
                     /locus_tag="PLG72_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79777.1"
                     /translation="MKKKLLLLCIMAIFLAAILSGCIPGDGTHTPSEPAGFLWGIWHG
                     WISPVSLVIGLFKKDIRIYEIYNTGWWYDFGYYIAIIGGCGGIALFRNRKGKG"
     gene            7397..7606
                     /locus_tag="PLG72_02890"
     CDS             7397..7606
                     /locus_tag="PLG72_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79778.1"
                     /translation="MDRIIVRNFRPEDIGRISELDRKYKKVFPDSAEVQAELYNSPEF
                     EGGRDVFCAFDETGALVGYALSHTG"
     gene            7719..8345
                     /locus_tag="PLG72_02895"
     CDS             7719..8345
                     /locus_tag="PLG72_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ79779.1"
                     /translation="MEIDNSAPVKNTKICCNYLTTETESIDYFLSKGFKPTDGIYNMA
                     RDLTKPVTEAVAPEGLEIIEWRMETEGEKARYIEAFNTVFPEKPWSVEGLDYFMQSDM
                     WACGTAITAFSGGDIASSIMLYWAPGPKEGERNAACTENIFVMPQWRRKGLGSCLIGK
                     GLSYLARHGAKTAFLEVRANNIKALDIYKRMGYEIIKEQKVLEYSIGN"
     gene            complement(8499..8819)
                     /locus_tag="PLG72_02900"
     CDS             complement(8499..8819)
                     /locus_tag="PLG72_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOJ79780.1"
                     /translation="MHSHYYLGKTTIDDRHWHNYEGMTSCDPNEPGHIHYMSGKTSLD
                     DGHVHEYHNATGPAIYVGGKHYHMYCAVTKEADGHVHKYEDATHKYYDEYYKKCPHKK
                     DLCK"
     gene            9028..9639
                     /locus_tag="PLG72_02905"
     CDS             9028..9639
                     /locus_tag="PLG72_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ79781.1"
                     /translation="MVIDPQHELLLNIYSIDYLLRCNVVELTDKILTLSPVSGKTDMF
                     QVLDPVVLISVGGSDIMTASAEVTAIDGRSGTVSLSMRDEAVSEERRIFERYPVSLEI
                     SARKKFSSKRMHMLVKNISLYGMMVVSGTELDKEEQIDIDLITEKNMFYLSGMVVWKR
                     EVANNCFEYGLQLTHYDVATRYLFQDYLNRQKTNFANMIPRAR"
     gene            complement(9734..11911)
                     /locus_tag="PLG72_02910"
     CDS             complement(9734..11911)
                     /locus_tag="PLG72_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79782.1"
                     /translation="MEELITRQKEYGAYIINSISSLILNTYQSLENVLSLCCATDSIY
                     RNLCAYYVTLSNTYGFSMVPGDISETGGSAISLLNEKGTAIPAAFSRQFAESGETAAP
                     AFVREAAGLQDQVRYYRQLNGISPNIRKLFFAADAYVTEKHAVEASKYLESIVDELCR
                     VFGHLEKTIGKLYGTDENNIYDAFIKAAFDMKEKGFDHTPALDAASYIFVKLKEISSQ
                     IASEYKHDAGIDFDYFEHVHSDRTAALSAYGTADSKPVDVSVSSDSVLSLPEELENSA
                     VKILEYAEVPEDKATYFLMNLTAFRNLKDKAASDESARAVRKAVADQFFDIYAAVFRK
                     AVRTKDDSRLIRMFLSYGYMDEKLLDVSQTMAIYKLAGMNHAADGTAGVYFMYEWLTE
                     IYDMTRDPSVNSFGNDYADTFRELKKQGRLTENDKAAYMSNREDRLDFEINNMIKTNH
                     KLVQGKISEYFPILHRDAAPYDPCRSFVSPSVICEKLNRMLGIDFSLFHREINYINAE
                     KGIEKELVMMKVMPDIILMPVYGTRAMMWQEIAGRVRSNPGRFILPVFTDENIDDMIV
                     RLAGNFRWELCRTMMGPAWNDITQSSLTSEYADYIQFYRKNRDLSEESKEKVKALIAK
                     YHNRLREIFTAEYEIWVNNESNGNPRLNRVARSIFVKYCPFSKPIREYLAKQPIYSDL
                     IMHFNIQRNKKVKELENRYKMYIKANNPLDPVLQENLDFYKDM"
     gene            complement(12385..14001)
                     /locus_tag="PLG72_02915"
     CDS             complement(12385..14001)
                     /locus_tag="PLG72_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOJ79783.1"
                     /translation="MDKISNIKAETERRRTFAIISHPDAGKTTITEKLLLYGGAIRLA
                     GAVKARKAGSFAVSDWMDIEKQRGISVTTSVMQFEYGGYFINILDTPGHQDFSEDTYR
                     TLMAADSAVMLIDGAKGVEAQTIKLFHVCRMRGIPIFTFVNKMDRASKDPFLLMEEIE
                     NVLGIRSYPMNWPIGTDGDFKGVYNRKLRQVELFEGGRHGSTIVNSIRGSVDDPVFAD
                     LLGSHYHNRLREEIELLDIAGDEFSMKKVLTGELTPMFFGSAMTNFGVRPFLEEFLEM
                     APCPGARMSDAGEIDPVSGNFSGFVFKIQANMNPTHRDRLAFIRICSGKFTKGMSVWH
                     VQAGKEVRLSQPQQFMAQERAIVEEAYPGDIIGVFDPGIFRIGDTLCEGGSHFRFEGI
                     PVFPAEHFARVSPADSMKRKQFVKGISELSEEGAIQVFRQPDSGMESVIVGAVGVLQI
                     EVLEHRLRHEYGVELRVSRLPYRHARWIVSEIDDPGRLNLTSSTMVVEDNWGRPVLLF
                     ENEWNIRWAEEKNNGLVLTDIQGAMSGQRA"
     gene            14312..>14961
                     /locus_tag="PLG72_02920"
     CDS             14312..>14961
                     /locus_tag="PLG72_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOJ79784.1"
                     /translation="MAYTKEQIKVTLDSGKTVTVQKGTALLELARQFSSLRRETIIAA
                     RVDNDIKELTYELKNDCNVNFIDLTEEDGMRIYRRSLYFIFMKAANDLFPDRRVIISH
                     AVGGGLYCELRGDRELEPHDVEMLEKRMHEIAEQAIPFEKREIPLPEAEALFESTGRM
                     DRYRAIEHRNKKLVTIYNCGGMDDYFYGYMAPDTGYIKVFGLKYYKGGLIIRFPDKS"
BASE COUNT         3717 a         3391 c         3877 g         3976 t
ORIGIN      
        1 caagggcagc cagtatatcg ccgttcagtt cataattcac ggatgtcgtt gagaagctct
       61 gtatccaggc gaattatgaa ctgaacggcg atatactggc tgcccttgag gagggcatgg
      121 aacttgagga gtcggagacg ggacggagag tgctgtgcca gctggctgaa aacgccagga
      181 tcgctgccag ggagcggatg gctatttgcc aggacaccgg gatggccgtt gtgttcatcg
      241 aactgggaca ggacgtccac atcaggggag gcagcctgag cgaagctgtc aatgaaggtg
      301 tcagaagggg ctatgaaaag ggctttctga ggaaatccgt tgtttcggat ccttttgaga
      361 ggataaacac aggggacaac accccggcag tcatccatta cgacatcgtg gaaggggacc
      421 gtatcagaat agccgttgcg cccaaaggtt tcggaagcga aaatatgagc ggtgtcagga
      481 tgctgaagcc ttccgacggg atagagggcg taaagtcctt tatactggaa atggtggaca
      541 gagcgggacc gaacccctgt ccgcccattg tggtcggagt cggcgtcgga gggacgatgg
      601 agaaggcggc gcttctggcc aaaaaagcat tgctgcggcc ggtggacagg cgcagcggtg
      661 atgaaaggat aagaaaactc gaggaggaaa tgaaggagct tataaacggg ctcggcatcg
      721 gacctgccgg gctgggcggc aggcttacgg caattgctgt gaatatcgag gttttcccga
      781 cccatattgc cggcctgccg gtagcggtca acataagctg ccacgtcacc aggcacgcgg
      841 aggcagtgat ctgaaaaggc tgtaatccgt ctccggagca accgtgctca gggccagcat
      901 agggttggga acaggtatgc cgaatggggg ggtatcggtg gataatgtaa aaagtataaa
      961 tacaccgctg acgagtgata tggcaaggca gcttaaagcc ggtgatcatg tccggatcag
     1021 cggggtcgtc tatgctgcaa gggatgcagc gcacaaaagg ttcatcgaaa tgcttgatgc
     1081 gggaaaggaa ctgccctttg atataaagga ccaggtcata tattatgtcg ggccgtgtcc
     1141 ggcaagacca ggccatgtta tcggatccgc agggcccacc accagcggga ggatggatgc
     1201 gtatgcgccg aggctcatag aacttggact tacggggatg atagggaaag gcctcaggag
     1261 cggcgaggtg gtggaagcga tgaaggaata cggggctgtt tatttcggcg ctgtgggcgg
     1321 tgccggagcg ttgctttcca aatgcatcat ttctgatgag atcgccgctt tcccggagtt
     1381 ggggcctgaa gccctgagaa ggctcgtagt caggaacttc ccggctgtcg ttgttattga
     1441 cagtgaaggc agggacctgt acagcaccgg acgtaagaaa ttccagcgtg tttttgtatg
     1501 aaacccggat cattggcggc acagtgtgcg gaataccgga aaagacagta ttgatgacga
     1561 taaaccaggg acgtttcaat atttgcaaat taccgggagg atggaagatg atcgggaatg
     1621 agagtataat taagcaattc ggagtcgcaa aggaatacga tgttgaagca gatatcaaac
     1681 gaagagtgga ttttttggcg aagtatcttg atgccaatcc gtgggcggaa ggttacctga
     1741 tcggcatatc cgggggtatc gacagcgcgg ttgtggcggc tttgctcaaa atgtcggttc
     1801 tggatacgaa taaaagagtt cttgccgttc tgaacccata ccattcaaag aacatagatt
     1861 atgcgcttga agtggtgcac gcattggacc tggaataccg cataagggat ataggcgaga
     1921 tcgttgatct cgcggtcaga agcttcaaca agaatgcttc gaagcttcag atcgggaaca
     1981 ggatggcccg ccagaggatg atcgaatggt atgatatcgc cgccgaagag agattgatcg
     2041 ttgcaggtac tgatcacgcg actgaatcgg tcatcgggta ctttaccaaa tacggcgatg
     2101 gcggtgtcga tataaatccg atcaggacat tggacaagag gcagataata cagctggcaa
     2161 aatatgaatg gcctttcggg aaaataccgg aaagcgttat ttccagggcg ccttcggcag
     2221 atctgtggga cgggcagacc gatgaaggcg agatagggat gacgtatgag gtcatttgtg
     2281 actatcttga aggcaaagat gtggataagg aatttgcgga aaaaattgaa cagatgtacc
     2341 ataaaagcat gcataagcgt cagatcccgt ttatatgact gacgcgaacc attgcaacag
     2401 ttggatgttt tatcgtgatt tgattatcgg acataaaacg gggtatattt taagtcagca
     2461 atagtgtata ataaactgta atttggaaag tgtaaactat attttttcag gaggttaagc
     2521 catggcaaag gtaactaaag atatgatcat ttccgatatc ctggcacttg acaggggcac
     2581 agcgccgata ctgctcaaaa tagggatgca ctgcctcggc tgcccggcat cggccggcga
     2641 aagccttgaa gaagcatgcg cggtacatgg cgtcgatgca gatatgatcg ttaaggaact
     2701 caacgattat cttgagtcca aacagggctg atatcagcct cagaaacatt cagctgccag
     2761 cttctgcgga gaccggccgg cgcgatgata cggccagcca aaatctgtgg ttgacaaaac
     2821 ggctgataat actgtatcat atatacctgt gtggtgaatg cgcacaagtt tatatgatac
     2881 ggatatgagc cattagctca gtaggcagag cacttgactt ttaatcaagg tgtccggagt
     2941 tcgaatctcc gatggctcac caaagagtct ttcattatat gtgaaaggct cttttttagt
     3001 tatatctgat ctttattttg acccatacgt ttacatgtgg gagaatgtaa atgatcggcg
     3061 caatggtata gatgcaagtg gtttacatga cgaagcgcaa atggcggttc catacctgta
     3121 tgttaataag actgaccaat ctataagaat ggagtaaaaa caatggagca taacctgaag
     3181 atcgtgttgc cgtatgaggg agtgaagaag tatataagtg cggtcagggc tgctgagagt
     3241 ccggatcatg gaaggctgtg gctggagtac gcgttcgacc cttactggaa tgagtgggca
     3301 gcagggcagt ttacagagga aaggacccgc agtgaaatgt cggatcctgt tacggacatc
     3361 gatgaactgg aaactgcggc agatttgctg attgcctctg atacagaagg actgctgagg
     3421 aacacatacg ataagataaa tacaacggtg gaagcacaga ttagcagtga taccgcagga
     3481 cggttctcat gtctggcgtg tgctgcccgc caggcatgag aaccgtccca ggtgctgtgc
     3541 agccttcagg ttacggggag atatcatatc ctgtaactgt ccagatccct gtgtcgtcct
     3601 gcctgatcag cttttcaaga taaacagcct ttatatgcgt tttttcgctg ataacttcaa
     3661 ttttcgcgct ggttccattg ctttcggcta tcacgaactc gttgtaatgt atcggatatt
     3721 ccccgactat gccttcaggt gagatctgca ggcttatgaa tacctgggcg acatagaccg
     3781 ggtcgagctt ccaaggagaa ctgcctgaat cggcggctat ttgctccgcc ttttcaatat
     3841 ccatgtccac gtccactgtg atttccggag tgtatttggc ggtatcgctg tctgctttgt
     3901 tattttcagc gatatctgcc gttatgtctt ccggcttatc cgggttttta actgtatttt
     3961 tatatccgtc aggatccact tctttgaaac ctgtatccgg accaaccgta agaagctcct
     4021 caggtattga ttctgcaaag tttatcaccg tataccttgt tgtatactgg atcgctttat
     4081 acattgaact ctgcttctgc agcgggatct tggttccctt gtcgacccag agtttgtatg
     4141 gaagcccgcc tttgggcgtt acttccatca cataagccgg ccttcccgct atgtcgtcat
     4201 caccgatgat ccttgctgat tctgcatttt gtagttcatc cacttcattc ccaagttcga
     4261 atatgaattc attggtctcg gaaaatacag ggaggacatt catcgcattt tcatcagtcg
     4321 gggactgcca gacattcttt ccgtcgctta ctgttttcag gcctttatat gagccttcca
     4381 gcacttcgat ataataattt ccacttttat ccgcccacac gtcaagaacg gattgcgtct
     4441 gcgtttcacc ggcttcattg ctgaatacga cttccagcgt accgtgatag gcttttacat
     4501 cgttatatgc ctgttctacg gcatagacca cgtacgaatt tttgatggga cccgcgaaat
     4561 tcgccagcaa agcaacagcc aacacagcgg cgatacctgc gaatccacct gcccaggtcc
     4621 tttttcctga ttttgccgta tgcctgtttt tctcggtaag gttttggatg atcctatgcg
     4681 gaaaatcttc atcaggcatg gcaggagact tcaggctctt gatctgcctc actgcatcaa
     4741 acatcctgtt taattcttcg gaatcagctt taactgtggg ctttttctca tcgttcaggc
     4801 tgtcgatgaa atcggaaagc ttttttacat tattcatggt gattctcctc ttcattcatg
     4861 ataattacca ggtttttcag cgcacggtac tgcaatacgc ggacatttcc ggcttttata
     4921 cccatcagcc ctgcagtctc ggataccgaa tatccttcta tgatcctgag ttctatgact
     4981 ttacgctgat cttcgtttaa tttttccagg gaacgcctga tgagcagatg cctgtttgcg
     5041 atatctgtga tatcatcgct taagactttt gtttgagcaa tggaatctat atcgatactt
     5101 gttcccctgg tttttttctt tcgccagcga tccctgatta tattcattgc tactgttttg
     5161 aaaaatgcca tatatctgcc ggccctgatt ctgtcaaatc cgggccacga cagcgacctg
     5221 acgtatgtat cctgcgtaat gtcttctgct tcttccctgt tttgaacttt gtaatatatg
     5281 aaacggtaca aaggctccca tgtttgctcg catatatcct caattgatct caagttatct
     5341 tcagccataa tatgtcacca tctttcagtg cgatcgtctc tactgactaa aacgaacggc
     5401 ggcgttatta tgttacatcg aaattgcaaa agatataaaa acaatgatcc gcaaggttag
     5461 ttgccagctt tatagtgtaa tatgatatca ttatcaatat aatagagatg ttgatgggag
     5521 agatatgcgt gacagccgaa cgcgtttata aaatgccgtt ttcaaaagtc tacccgctgc
     5581 tcgtccagaa ggcggagcgc aaaggacgca ccaggtccga ggtagatact gttatttgct
     5641 ggctgacggg atatgatgaa tcgggtttac aggagcagat tgcaaagaat gtggattacg
     5701 aaaccttctt caatgaggct ccgctgataa atccgaacgc ggtaaaaatc accggcgtga
     5761 tctgcggcca tcgcgtagag gagatcaggg acccgctcat gcagaagatc cggtggctgg
     5821 ataaactggt cgatgagctg gccaaaggca aaccaatgga gaagatcctg cggagttaac
     5881 taaaaaggta aggaccgcat gaatggttga tcatttatga ggggattatg atatctttta
     5941 ttgtatattg aaggaatatt tgctatttta aagtataata atcgaatatt acatactgca
     6001 tgttcatacg gatttcgaaa atgttttgtg gggtgagaag tatgccggac atggagagga
     6061 tggacaaaca cttgctggac ctgggcggga cctatatgct caatcacgca agacgctgcc
     6121 ttgttatcgc gaaaaagctg gccgaagaag agaatcttgc atatgacgaa gatatacttg
     6181 cgttttcctg ttatttccac gatataagcg catataagcc gtaccgtccc gacggaccgt
     6241 tcgatcacgc ggaggaatca gccaaaatag tgcccgctat cgcagaagag tatggtatca
     6301 atgatgccga caagatcagg atcattgtgg aagcggttaa atatcatgac aaacgcggcc
     6361 agggcacagc caatgaaaca atactggtaa gaaatgccga tgcggtcgat tatcttggtt
     6421 ttgtcgccgt ggcacgtgat ttctcaaagc agcccaacga gatgacaaaa gcagttgcag
     6481 cattgaaaaa gcatctggcc ggtttttctg cccttcttga actggattcg gccagacgca
     6541 tggccggacc caggatcaaa aagctggaga catttttgca tgagttcgag caggagacct
     6601 tcggccttta ttaggaaaca tgtataaatg cggggccagg caagacaaac agcgttttcc
     6661 aggaaattaa acctatagcg aaagaatggc ttggcaataa ctgttttgtg ctgtataaat
     6721 gtgaatgtac agatgaaatg tttttcagca gggatctgta ttattacctg cgtgatgcat
     6781 tttcagcgtt gttccatgta tatgtcttgc ttaaggacgc attgcgggat tgatctttcc
     6841 cggtgagaag ggagtgtatt gatatgaaaa agaagctgtt gcttttatgc atcatggcaa
     6901 tatttctggc tgccatactg tcgggatgca ttcccggaga cgggacccat acgccttcgg
     6961 aaccggcagg cttcctttgg ggcatctggc acggctggat atcgcctgtg tctcttgtga
     7021 tcggcctttt taagaaagat ataaggatct atgagatcta taatacagga tggtggtatg
     7081 attttggcta ctatatagca attataggag gatgcggggg gattgcactg ttccggaaca
     7141 ggaaaggtaa gggctgaggg acggaaatca acagtcctga agattcatcg taccgcattg
     7201 ggcagatcga gcggtctgcc ggctgcaagg cagtcgtatt cagaaacaag ctggactggg
     7261 atgcctgcgg cagccaatga gatataccgt aagcgggctt ggacaggaaa tcggtcgata
     7321 tccggcagtt tcagaagctt ttggtatcag caaaacttaa ggtatcagtt tataaaatga
     7381 aaggagaagt aaaattatgg acagaataat cgtcaggaac ttcagaccgg aggatatcgg
     7441 ccggatatcc gagctggaca ggaaatataa aaaggtgttt ccggattcag cggaggtcca
     7501 ggccgaactc tacaattctc ccgaatttga gggaggcagg gacgtctttt gcgcttttga
     7561 cgaaacgggc gcattggtgg gatatgcgct atcccatact ggctgaagcg gggagtggat
     7621 atccgaatgt tttatgggcc gatataaagg tcgacccgga tttccgggat aaaataccgc
     7681 ttcgggatct gctttttgat agtatggtgg ctcgggccat ggagatcgat aacagcgcgc
     7741 ctgttaagaa tacgaagatc tgctgcaatt atctcacgac agagaccgag agcatcgatt
     7801 atttcctttc aaaaggattt aaacccactg acggcattta taatatggca agggatctga
     7861 ccaaacctgt gaccgaagcc gtggcccctg aaggcttgga gatcattgaa tggaggatgg
     7921 aaacagaagg ggagaaagcc agatatattg aggcatttaa tacggtcttc cctgagaaac
     7981 catggagtgt ggaaggcctg gattacttca tgcagtctga tatgtgggcc tgcggtactg
     8041 cgatcactgc tttcagcggc ggcgacattg ccagcagcat aatgctgtac tgggcgcccg
     8101 gcccaaaaga aggggaaaga aatgcggcgt gtacggagaa tatattcgtc atgccgcagt
     8161 ggaggagaaa aggactgggc agctgcctga tcggcaaggg gctttcttac cttgcgcggc
     8221 acggcgcaaa aacagccttc ctcgaagtgc gggccaacaa tatcaaggca ttggacatct
     8281 acaagcggat gggatatgag atcataaaag agcaaaaagt gctggagtat tcaataggta
     8341 actgattgca aagtccatcg ccatggtggt gaaacggtat aaggcagctt cctgcatacg
     8401 gatgctgcct taaattttgg atatggctgt attatagcat gtgcatggct gtttgcctgt
     8461 caggtatcat cctaaaactt tactgcttcc ttgccacatc atttgcacag gtcttttttg
     8521 tgcggacact ttttgtaata ctcatcatag tacttgtgtg tcgcgtcctc gtatttatgc
     8581 acgtggccgt cagcttcctt tgtaacggcg cagtacatgt gatagtgttt tccgccgacg
     8641 tatatcgccg gtccggtggc attgtggtat tcatggacat gcccgtcgtc aagcgaggtt
     8701 ttcccgctca tgtaatggat gtggccgggt tcgttcgggt cgcagcttgt cattccctca
     8761 taattatgcc agtgccggtc atctatcgtc gttttgccca gatagtaatg tgaatgcata
     8821 gaaaccacct ccgatatatt gtatattatg ttaactccgc ttcttgtgtg aatggtccgg
     8881 gcaaatagcc agtttgataa atgcgtaaca ttctgttaaa atatatatgt aaactgctaa
     8941 acaatttcca taaagaatat agaaatgagt tccgaatatg tttgatctta tccgctaatg
     9001 ttacgtcata gtgttcctgg aggtaatatg gtaatagacc ctcaacatga attgcttctt
     9061 aatatctaca gcatcgatta ccttttgaga tgcaacgtgg tggaattaac ggataaaatt
     9121 ctcacgcttt cgccggtctc gggaaagaca gacatgttcc aggtccttga tcccgtagtg
     9181 ctgatttctg tcggcggcag cgatataatg accgcatcgg cggaagtgac cgccattgac
     9241 ggccggtcgg gcacagtttc gctgtccatg cgcgatgagg cggtgtctga ggaacggcgt
     9301 atctttgaaa ggtaccctgt atcacttgag atcagcgcca ggaagaagtt ctcgagcaaa
     9361 cggatgcaca tgctcgtaaa gaatatcagc ctgtacggca tgatggtggt ttccggtact
     9421 gagcttgaca aggaggagca gatagacatc gaccttatca ctgaaaagaa tatgttttac
     9481 ttaagcggca tggtcgtgtg gaaaagggaa gtggccaaca actgtttcga atacggcctt
     9541 caactgaccc actatgatgt ggccaccagg tatctgttcc aggattatct taacagacag
     9601 aaaaccaatt ttgcaaacat gataccaagg gccagatgat catggccgca gacctcattg
     9661 gatgcagatt gcagccgctg caggtgatcc ggccgctttg aggtattgcc tggtgcagtt
     9721 tgcatcggtc cggtcacatg tccttataaa aatccaggtt ctcctgcaat accgggtcaa
     9781 gcgggttgtt agccttaatg tacatcttat acctgttttc cagttctttt acttttttgt
     9841 tgcgctggat attgaaatgc attatcaggt cggaatagat cggctgtttc gccaggtatt
     9901 ccctgatagg cttggaaaac gggcagtact ttacgaatat gctccttgcg accctgttga
     9961 gcctggggtt cccgttggat tcgttgttta cccatatctc gtattctgcg gtgaatatct
    10021 cgcgcagcct gttatggtat tttgcgatca gcgccttgac tttttccttt gattcttcgg
    10081 aaaggtcgcg gttcttgcgg taaaactgta tataatcggc atattccgat gtaagcgaac
    10141 tttgcgttat gtcgttccag gcagggccca tcatggttct gcacagttcc cacctgaaat
    10201 tgcccgccag cctgacgatc atatcatcta tattctcatc cgtgaaaacc ggcagtatga
    10261 atcttcccgg gttgctcctg acccttccgg cgatttcctg ccacatcatt gccctggtgc
    10321 cgtaaaccgg catgagaatt atgtcgggca tcactttcat cattacaagt tctttttcga
    10381 tgcccttttc ggcatttata taattaattt ccctgtggaa gagggagaaa tctatcccga
    10441 gcatcctgtt cagcttttcg cagatgacgg aaggcgaaac gaacgaacgg cagggatcat
    10501 acggcgcagc gtccctgtgc aggatgggga aatactctga gattttcccc tgcaccagct
    10561 tgtggttggt ttttatcatg ttgttgatct cgaaatccag cctgtcttcc cggttgctca
    10621 tataggccgc tttgtcgttt tcggtcagcc gcccctgctt tttaagctca cggaatgtat
    10681 ctgcgtagtc gttcccgaag ctgttgacag aaggatccct ggtcatgtcg tatatttcgg
    10741 tcagccattc atacatgaag tatacaccgg cagtgccgtc agcagcgtga ttcatccctg
    10801 ccagcttgta tatggccatc gtctggctga cgtcaagcag tttttcgtcc atataaccgt
    10861 aggagaggaa catcctgata agcctggaat catcttttgt cctgacggct ttcctgaaga
    10921 ccgcggcgta tatgtcaaag aactggtctg ccactgcctt cctgacagcc cgcgcggatt
    10981 cgtcggaagc ggctttgtct ttcaggttcc tgaaagcggt caggttcatc aggaagtaag
    11041 tcgccttgtc ttccgggacc tcggcgtatt ccagtatttt gacggcactg ttctccagtt
    11101 cctcgggaag gctgagcacc gaatcagatg aaacggatac gtcgaccggt ttgctgtcag
    11161 ccgttccgta agcggacagt gcggctgtcc tgtccgaatg cacgtgctcg aagtaatcga
    11221 agtctatgcc tgcgtcatgc ttgtattccg aagcgatttg cgacgatatt tctttcagtt
    11281 ttacaaatat gtatgaagcg gcatccaggg ccggagtatg atcgaagcct ttctccttca
    11341 tatcgaacgc agcctttatg aatgcgtcgt atatattgtt ctcatcagtc ccgtaaagct
    11401 tgccgatcgt tttttccaga tgcccgaaga cacggcaaag ttcgtcaacg atgctttcga
    11461 ggtacttgga ggcttcgacg gcgtgctttt cagtcacata tgcatctgcc gcgaagaaca
    11521 gcttccttat gttgggcgaa ataccattca actggcggta atagcgaacc tggtcctgta
    11581 atccggcggc ctcccgtaca aacgcgggcg cagccgtctc tccggactcc gcgaactgcc
    11641 tgctgaatgc cgcaggtatc gcggtacctt tttcgttcag cagggatata gccgatccac
    11701 ccgtttcaga tatgtccccc ggcaccatgc tgaatccgta cgtgttgctt agcgtaacat
    11761 agtaagcgca cagattgcgg tatatgctgt ctgtggcgca gcatagcgag aggacgtttt
    11821 cgagagactg gtatgtgttc agtatcaggc tgcttataga gttgattata taagcgccgt
    11881 actctttctg cctggttatc agttcctcca catcctgcct gttttgcgca gggaaggaat
    11941 agaagcagca gtccgtttca gttacatatg tcagtaagct cgtcccgccg tcgaacagtt
    12001 cattggctcc tatgaagatg ttctggtcaa catcgaacag tttatatccg gcgcgggctg
    12061 ggccttccgg acctgtggat ccctgctgcc cgcacgtggg ggaaatatat gcttccagtt
    12121 ttccttgcag cagcagttta agtgaatcag gtttttcacc ttcgattatg atttttggtt
    12181 tgcgggcacg ttgaacgtgc cgttgctgac tggttgagga ttcatttgac cgctccgtta
    12241 tgacattata gtgaacatta gctgcaaaaa tgacagtatt tatctttatt tttctttata
    12301 ttatacatta attttcagcc atgttaaagt aatgcagcca tttgtcagac ggtccggcca
    12361 ggtcggcatc gcaggcgccg cgtttcaggc acgctgtccg gacattgctc cctggatgtc
    12421 tgtcaggaca aggccgttgt tcttttcctc agcccatctg atgttccact cgttttcaaa
    12481 gagcagcacc ggccttcccc agttgtcctc cacaaccatc gttgagcttg taaggttgag
    12541 ccttccagga tcgtcgatct ctgagactat ccatcttgca tgcctgtacg ggagacggct
    12601 gaccctgagt tcgacgccgt attcatgcct gagcctgtgt tcgagcacct cgatctgcag
    12661 taccccgaca gcgcccacta ttacggactc catgccggaa tccggctgcc tgaacacctg
    12721 tattgcccct tcttccgaaa gttcgctgat ccccttgacg aactgtttcc ttttcatgga
    12781 atccgcgggg gagaccctgg caaagtgttc cgcagggaat acggggatgc cttcgaacct
    12841 gaaatggctg ccgccttcgc ataaagtatc gccgatcctg aatatgcccg gatcaaacac
    12901 gcctattata tcgcccggat acgcctcttc aactatagcc ctctcctggg ccatgaattg
    12961 ctggggctgc gacagcctga cctctttgcc ggcctggacg tgccagaccg acatgccctt
    13021 tgtgaatttg cccgaacaga tgcgtatgaa cgcaagcctg tccctgtgtg tcgggttcat
    13081 gtttgcctgt attttgaaaa caaatcctga gaagttcccg gatacgggat cgatctcgcc
    13141 tgcgtccgac atcctcgcac cggggcatgg agccatttcc aggaattcct cgagaaacgg
    13201 ccggaccccg aagtttgtca tggcgcttcc gaagaacatg ggggtgagtt ccccggtgag
    13261 gacttttttc attgaaaact cgtcgcctgc tatatccagc agttcgatct cctcccgcag
    13321 cctgttgtgg taatggctgc ccagaaggtc cgcaaataca ggatcgtcga cagatcccct
    13381 gatagagttc acgatggtgc tgccgtgcct gcctccttca aaaagttcga cctgcctgag
    13441 ttttctgttg tacacgccct tgaaatcgcc gtccgtaccg atgggccagt tcatcggata
    13501 ggaacgtatg ccgagcacgt tttctatttc ctccatcagc aggaaaggat ccttgctggc
    13561 cctgtccatt ttattgacaa acgtaaatat cgggatcccc ctcattctgc agacatggaa
    13621 cagctttatc gtctgcgctt cgactccttt tgcgccgtcg atgagcatca ccgcgctgtc
    13681 ggccgccatg agcgtcctgt aggtatcttc gctgaaatcc tggtggccgg gagtgtcaag
    13741 tatgtttatg aaatatccgc catattcgaa ctgcatgacg ctggttgtga cggatatgcc
    13801 cctctgcttt tctatatcca tccagtccga aacggcaaag ctgcccgctt ttcttgcctt
    13861 gaccgcgcct gcgagcctga tggctccgcc gtaaagcagg agtttttcgg ttatcgtggt
    13921 ttttcccgcg tcagggtgcg atattattgc gaaggtcctt cttcgttctg tttcggcctt
    13981 tatattgctg atcttgtcca tttcagtctt ttctcccatt tgtactatca ttcccagaaa
    14041 gaatttttca tttcatatta gcgcataata aacattcctg tcaaacactt gataaatata
    14101 ctataccggg gcgtatcggg gagcgtatcc tccgcttcgt tgaaacgcaa cagaagcgaa
    14161 aaattttctc tcatgtgtta taacgaggct tttgtatacc tggggcctcg ttgaaacgca
    14221 acagaagtga gaaaaatttc tacaatcgaa atttttccct tatgcgttat aataaaaata
    14281 tagtatatcc ggaaaagtga ggagaaaaca gatggcatat acaaaagagc agataaaagt
    14341 cacgcttgat tcaggaaaaa ccgtaactgt gcaaaagggt acggcgctgc tggaactggc
    14401 gcggcaattt tccagtctcc gccgcgaaac gataatcgcg gcaagagttg acaatgatat
    14461 aaaggaactg acatatgaac tgaagaacga ctgcaatgtg aacttcatag accttacgga
    14521 agaagacgga atgaggatat acaggaggag tctgtatttc atattcatga aggcagccaa
    14581 cgacctgttc cctgacagga gggtcattat cagccatgcc gtaggcgggg gcctgtactg
    14641 tgaattgaga ggagacaggg aactcgagcc tcatgatgtg gagatgctgg aaaaacgtat
    14701 gcacgagata gcggagcagg cgatcccgtt tgagaagagg gagatacccc ttccggaggc
    14761 cgaagcattg ttcgaatcca ccggcaggat ggacaggtac cgtgccatag agcacaggaa
    14821 caagaagctt gtcacgatct acaactgcgg aggtatggat gactatttct acggctatat
    14881 ggcgcctgat accgggtata taaaggtttt cgggcttaaa tactataaag gcggccttat
    14941 tataaggttc cccgataaat c
//
LOCUS       DAOSCC010000029        14845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2741_length_14845_cov_4.557945, whole genome shotgun sequence.
ACCESSION   DAOSCC010000029 DAOSCC010000000
VERSION     DAOSCC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14845
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2741_length_14845_cov_4.557945"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..2817)
                     /locus_tag="PLG72_02925"
     CDS             complement(82..2817)
                     /locus_tag="PLG72_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ79785.1"
                     /translation="MGKTVELLAPAGNAESLHAAVENGADAVYLGGKLFNARQQASNF
                     DMPELRDGLRYAHARGVSIYLTLNTLVNNEEISQALAYAEEAYNAGIDGIIVQDLGLA
                     ALIRKTLPELPLHGSTQMTVYDLEGVRMLEEMGFRRVVLARELTLEQAASIARNTSLE
                     VEIFVHGALCVCYSGQCLMSSMIGGRSGNRGKCAQPCRLAYTLVGPGGRRGSADRPRY
                     LLSPKDMNTLEYMPGIVSSGVRSLKIEGRMKSPEYVATVVRIYRKYLDIAMGQANRGG
                     AAKLDIDEKDRHDLLQIFNRGGFSSGYLKGKTGARMMSYDKPNNSGVCIGSVLSYDRK
                     RRTVNIKLDEALATGDGIEIWTGGSDSPGGTVSMIKKDGLNVKGAVEGDIVEVGFFSG
                     NISPGRKVFKTTDIELTRESRRTWSDGNTRRVRIKGAAVLENGKPMALAAEDDDGHRI
                     TVSGTVPAETAVNRPITRERLIDQLSKTGSTPFDFARLDVEMQDGLTIPVSEINEVRR
                     KALDALYEARADRYPERRRKRAGRGQDSDNGEFAGSGQQISEPNDLHDEQVPNLASST
                     ALRTSYDTGMNQAVDPGRKIKRPSISLYFYRWDDGRDIAGLGADRIYVPVTAAWKPGF
                     RHAAETARKTATELFCWLPSITKGNYEKLIGRFIAEYSGSGDIDGILAANAGTVHKLS
                     GTSGLRLAGDISLNLFNSCSVAEIAGYGLESAAISVELTIRQISGLAAGLRQAVSISD
                     FGQARGKCQGGPDGEGSRSGSMDRRRSIDLEAAVYGRLPLMISEYCPVGCIEGGFDGT
                     AKCSGCCGKGVYALRDRLGVEFPVLCDNVDCRSTILNSKALFVPGSLGSLAEAGIGIF
                     RLYIWDEDDDTVKELVRLYRSNLTGNGGDDGTDFVRLTEKIKAAGFKKGHYYRGV"
     gene            complement(3210..3626)
                     /gene="zapA"
                     /locus_tag="PLG72_02930"
     CDS             complement(3210..3626)
                     /gene="zapA"
                     /locus_tag="PLG72_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOJ79786.1"
                     /translation="MTAKNKVELRIAGKDYTIVGSEPEEYIHRVGLYVDKKMTDILKI
                     NNKLSTSMAAVLTAINVVDDLFKSRDSEQELKNQLKKATEELEKLKRENKALQQENTI
                     LGNQNTELKLELAKREAELNEVRNTLDKAERTKEQD"
     gene            complement(3786..>5191)
                     /locus_tag="PLG72_02935"
     CDS             complement(3786..>5191)
                     /locus_tag="PLG72_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ79787.1"
                     /translation="ALVVVIFILISVFVGRYVKVGPDEALIVSGRKKRLPNGQVVGFR
                     IVKGGATFVWPILEKAKTISLRIMPLDLNSSAYTSQGVQVSVDGVAQVKIDSTTEAIA
                     TAAEQFLSLKEEEIRRIATQTLEGHLRSIVGNLTVEEIMANRDAFAQKVQELAASDLA
                     NMGLKIISFTIREIADKNGYLESLGKAQIAKVQRDAVIGQAEAKRDADIKSAEAMQAG
                     QTARYMAETKVAEANRDKEMKMAEYQASINEKKAEADLAYDKKKYTLQQEVEKEAMQV
                     EIVKKQKEIELQEQEVLRKAQELDATIRKPAEAEKYRTELSAEADKVKKSMEAEAQAF
                     AIKAEGAAKAEAIKAAGLAEAEVIKAKGEAEAEALAKKAEAYKQFNEAAIAQMVIEKM
                     PEIAAAIAQPLAKTEKIVMIGDSGASKITRDITEIMAQLPETIKGLTGIDLTNILRNY
                     AEPQKAEQADEAKEQQG"
     assembly_gap    5192..5291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5492..6274)
                     /locus_tag="PLG72_02940"
     CDS             complement(5492..6274)
                     /locus_tag="PLG72_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79788.1"
                     /translation="MSFILGGHGDSGSDGIDIGHDADIGGMDAGGVDIGDADIGSVDV
                     GDADIGSGIGADADGFDAAGPDADMDIGAGHAIDAAHGAETGQAAEAHHHDASDTTGS
                     PSPFSPVVMASAISTFGAVGIISMKGFGLSGLASTFVALGFAGAIGAAIFFGIVKFMY
                     GSQSNSLFSLDDLVGAEADVITTVPAKGLGEIAYTIKGIRYTLAARSLEGCEIRRGTP
                     VIIREIAGNAAVVQQKLTIDDIELEEGVLKRKEDITETKNRN"
     gene            complement(6598..8310)
                     /locus_tag="PLG72_02945"
     CDS             complement(6598..8310)
                     /locus_tag="PLG72_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79789.1"
                     /translation="MHTSYTNRFQRNFMIFVNEDSGFEEGGKPSGYIKLEIREGKGKL
                     ETVIHNLKEGKGKFLYALYLVRQSGDRTDYVRLGDITHTAGRAEFRCGFDPMYVGGLP
                     YPIDQYDIAAVVVEYGDGPANNVICPLAAYRRKAAGWRNGLRKALQAADKTAGIQKTD
                     SGLFHLRNAGTVHPGPAEYTATGKTEAASHQDSGQKKPVSYPFSGQADPRYQNEPADS
                     LQPLDFSGQQEHLTDGSAGREPYALPDQKPFAADGSGTLARPGKEAGPETGSGPEFGP
                     GPGHEREPEPETEPVLEQKQEHVQEQEQKPGQELKSEPEAGPEIGPGQEQKQDHGPGR
                     EAEPKQEQKPEPVQPAAGGRPGPGVRPGDGISGTEDIKDTSHGQTGGRTDEVSPPNLH
                     KVNTDCVYLNGNICDALVNNRQDPDPCGSCRINQGRASAKANPPADIDSLEYDLDLNF
                     EVCDPFRSRRSDYKWWKVTNPVNLNNILYQNNIRSPLMFNPAVMLAHYRYKHLIIGIF
                     RHKNGRKYVVCGVPGKYAEDMKPFGEMNTWVQAQGTRPRYGAFGYWLVYIDPSDGKVL
                     HPYR"
     gene            8828..10621
                     /locus_tag="PLG72_02950"
     CDS             8828..10621
                     /locus_tag="PLG72_02950"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOJ79790.1"
                     /translation="MTKNKKLLDWVKEMAEMCQPDKIYWCDGSEEENKRLLQEMVDAG
                     MAIPLNPEKRPGCYLFRSHPSDVARVEDRTFISSKTQDDAGPTNNWIDPVELKKTMKE
                     LYTGCMKGRTMYVVPFSMGPIGSPISKIGIELTDSPYVVVNMRIMTRMGKHVLEALGD
                     NGDFVPCLHSVGAPLAEGQKDAAWPCAPMEKKYISHFPEERTIWSYGSGYGGNALLGK
                     KCFALRIASVQARDEGWMAEHMLILKITDPKGRKFYVTGAFPSACGKTNLAMLIPTIP
                     GWKVETIGDDIAWMRFGEDGRLYAINPEAGFFGVAPGTSMESNPNAMLSCAKNTIFTN
                     VALTDDGDIWWEGIGTDAPDHLIDWKGNDWTPASGEKAAHPNARFTAPASQCPVIAPE
                     WEDPNGVPISAILVGGRRPSTVPLVHESFDWNHGVFMGSIMGSEITAATISANIGKVR
                     RDPFAMLPFIGYHVGDYLQHWLDMGKKTTPDKLPKIFYVNWFRKDKDGNFLWPGFGDN
                     SRVLKWIVERVSGEGKAVETPIGYMPTVDAIDTTGLGISEETMKELLKVDRNEWLKEV
                     ESIREHYASYGPKMPKELYAQLDALEKRLKG"
     gene            complement(10700..11569)
                     /gene="rsmA"
                     /locus_tag="PLG72_02955"
     CDS             complement(10700..11569)
                     /gene="rsmA"
                     /locus_tag="PLG72_02955"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ79791.1"
                     /translation="MEADIKSIFRKLNIHPSRSLGQNFLTDGWVLQKIAEAAELTPND
                     TVLEIGPGLGSLTSYLAEDAGCVVAVEIDKRLVQVLHDLLIGYRNVHIINADILRLDL
                     KKELAPFMTSPDGTLRSLKVVANLPYYITTPVIMKLLEDGLEAECMVIMVQKEVAARM
                     MAKPGGKDYGALSVSVGYYSRPTVITDVPPDCFTPRPGVDSTVVRLDLYKTPPVELKD
                     RDLFFKVVKAAFGQRRKTLLNALSNAGYIGIGKDQVRLLLDKAGIEEMQRGETLSIQQ
                     FARLANEIYDLRR"
     gene            complement(11588..12655)
                     /locus_tag="PLG72_02960"
     CDS             complement(11588..12655)
                     /locus_tag="PLG72_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOJ79792.1"
                     /translation="MLTLVRSFKRFFSLKRHLLLFAAAAVLSSAAGYGVFSWLEKDVV
                     INDNGRVISFRTMKNTFGEVLDQLGIEVAEYDYVSASADTKLQTAKSNELYIKRAVPV
                     YITADNKRTEVMTYRDTVEEMFEDSPVKPGSKDRLAGASMEDRITSGMELRIIRVSED
                     YVSETEEIPFVVHRRPNKMMDEGTERVYQEGREGVLQKVYKVVSEDGVQVTKQLVSET
                     ILQNPVNMIIEYGTVLNFRTSRGEVVRYSKVLDMKATAYTASLKDTGKAPDHPLFGIT
                     ATGMKARKGVIAVDPKVIPLYTKLYVEILDKNTPDYGFCIAGDVGSAIKGNKIDLYYD
                     SQEYVDRFGVKKVRVYILNDQ"
     gene            complement(12927..13583)
                     /locus_tag="PLG72_02965"
     CDS             complement(12927..13583)
                     /locus_tag="PLG72_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79793.1"
                     /translation="MKEDRRMKDISKAVPGGTMPYVGPKQDLKAAPGTSPAQTLPKES
                     AVKETPKTSPEKTVSGVSPEKAADVLSSKTVSDLPSMQAMPGTLPAQTVQDKMAAQSA
                     EGGAPANKMPVQPGPYGVMPMMQQIPIICCPYLMNLQCPMIYGANLMGLNAMNGAMPF
                     GAGPAADDMMPYTGGGSVPSITGGPMAGIPGSNMYPPYGMGALPSAGSQYFPMSGMDY
                     "
     gene            complement(13673..14374)
                     /locus_tag="PLG72_02970"
     CDS             complement(13673..14374)
                     /locus_tag="PLG72_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79794.1"
                     /translation="MKKFFLVMIFALLIAVFIAFNYLLWDRESKLAEIKNLESVNASY
                     SASISVQKREIGALEDEVKSLGNQIEQYKAEIDRLQKEKEQMAGEKLQEEAELRAKID
                     LINILKENADIQFLSKPVVSWVEALNSGSFDEAYAIEFEGVPQKDRNISLSAYVEQME
                     STVQKIEITEVRVDRYRGSSNGEIYLDVTLNVGLAENADESVSRFAEGENEIYVKLDY
                     SKEKKGFIISSINIY"
     gene            complement(14396..>14845)
                     /locus_tag="PLG72_02975"
     CDS             complement(14396..>14845)
                     /locus_tag="PLG72_02975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ79795.1"
                     /translation="QKTWFAKQISLAGELKLPVIVHDRDAHEDTLNILKSEGARDVGG
                     VLHCFSGSVEMAREVMELGFMISIAGPATFRNARKLPDVIRYVPDDMLLIETDSPYLA
                     PEPHRGRRNEPAYVKFVAEKIAKIKGKPFEYICETTTANAKRLFGIP"
BASE COUNT         3113 a         4189 c         3404 g         4039 t
ORIGIN      
        1 tgacggttct cgcgtcccgg acacggggac ggttctgctg cttcgattat ctccgtatat
       61 ccttccgcat ccgtaccgct tttacacgcc cctgtagtaa tggcctttct taaaacccgc
      121 agctttgatc ttttcagtca gccgtacaaa atccgttcca tcatcgccgc cgtttcctgt
      181 caaattactc ctgtatagtc ttacaagctc ttttacagta tcgtcgtcct catcccatat
      241 ataaagccgg aaaatgccaa tgcccgcttc agcgaggctt ccgagcgaac caggcacgaa
      301 cagcgctttt gaatttagaa tggtgctcct gcagtcgaca ttgtcgcaca ggactggaaa
      361 ttccacgccc agcctgtccc gcaatgcata aacgcctttc ccgcagcagc cgctgcattt
      421 cgccgtgccg tcaaagccgc cttcgatgca gccgacaggg cagtactcgc tgatcatcag
      481 cggcagcctg ccgtacacgg ctgcttccag atctattgac cgacgccggt ccatggatcc
      541 ggaccggctg ccttctccat ccggtccacc ctggcatttt ccacgtgcct gcccaaagtc
      601 agaaatggaa actgcctgcc gcaaaccggc cgccaaacct gatatctgcc ggatggtcag
      661 ttcaactgat attgcggcgc tttcgagccc atacccggca atttctgcaa ccgagcacga
      721 gttgaagagg ttgagggata tgtcgccggc cagccgcaat ccgcttgtcc cggataattt
      781 atggactgtc cctgcatttg ccgcaaggat gccgtcgata tcaccactgc cgctgtattc
      841 agcaatgaac ctgccgatca gtttttcata gttccctttt gtgatggaag gaagccagca
      901 gaataattcc gttgccgtct tccttgctgt ttccgcagcg tgcctgaatc ccggcttcca
      961 ggccgccgtg accggcacat atatcctgtc ggcgccgagt cctgcaatgt cccttccatc
     1021 atcccacctg taaaagtaca gcgatatcga tggccgtttg atttttcgtc cgggatcgac
     1081 tgcctgattc atgccggtgt cataagatgt acgcagcgcc gtgctgctcg ccaggttagg
     1141 aacctgttca tcgtgcagat cattcggctc tgaaatttgc tgcccgctgc cggcaaactc
     1201 gccgttatca ctgtcctgac ccctgccggc gcgtttccgc cgcctttccg gatacctgtc
     1261 cgccctcgct tcatataatg catcaagcgc tttccttctg acttcattga tctcacttac
     1321 cgggatggtc aggccatcct gcatttcaac atccagtcgg gcaaagtcaa aaggcgtcga
     1381 acctgttttg ctcagctgat ctataagcct ttccctggta atcggcctgt tcacagcagt
     1441 ttccgccggg actgttcctg aaactgttat cctgtgcccg tcatcgtcct ctgccgcaag
     1501 cgccatcggc ttcccatttt ccaggacggc ggcacctttt atccggacgc gcctcgtatt
     1561 cccgtcgctc catgtccttc gggattccct ggtcagttcg atatccgtgg tcttgaaaac
     1621 tttcctgccc ggtgaaatat ttccgctgaa aaagccaact tcaacgatgt cgccctccac
     1681 agcgcccttc acattcaggc cgtccttttt tatcatgctg acagtgccgc cgggactgtc
     1741 ggacccgcct gtccagatct ctatcccgtc gccggtggcc agcgcttcgt ccagctttat
     1801 attcacggtc cggcgtttcc tgtcgtatga caatacactt ccgatacaga ctccgctgtt
     1861 gttcggtttg tcatagctca tcatgcgggc gcctgttttc cctttcaggt agcctgaaga
     1921 aaacccgccc ctgttgaata tttgcaaaag gtcatgcctg tctttttcat caatgtccaa
     1981 ttttgccgcg ccgccccggt ttgcctgccc catggcgatg tccaggtatt tcctgtatat
     2041 ccggactacg gtggccacat actccgggct cttcatcctg ccttctatct tgagcgacct
     2101 gacgcctgaa gaaacgatcc cgggcatgta ttccagcgtg ttcatatcct tcgggctcag
     2161 caaatacctc ggccggtccg cagaccctct tctgccgccg ggaccgacaa gcgtatatgc
     2221 aagcctgcaa ggctgtgcgc attttcccct gttgccgctc ctgccgccga tcatgctgct
     2281 catgaggcac tgccccgaat agcagacgca caacgctcca tgcacgaata tttcaacttc
     2341 caggctagta ttgcgagcga tcgatgcagc ctgctccagg gtaagttccc tcgcaagcac
     2401 gaccctcctg aatcccattt cttcaagcat ccgcacgcct tccaggtcgt acaccgtcat
     2461 ttgcgtgctc ccgtgcaggg gcagttccgg aagcgtcttc ctgatcagag ccgccaggcc
     2521 aagatcctgc actattatgc cgtctatccc cgcgttataa gcctcttcgg cataagccag
     2581 ggcctgggag atctcttcat tattgaccaa cgtattgagg gtcagataaa tgctcacacc
     2641 cctggcatgg gcatatctca acccgtccct gagttcaggc atgtcaaaat tgcttgcctg
     2701 ttgccttgca ttgaaaagtt tgcctccaag gtaaaccgca tctgctccgt tttcaactgc
     2761 cgcatgcaaa gactccgcat tgcctgccgg agccagcaat tctactgttt tccccatggt
     2821 ttctcctata tgttttcaac ctgttcctgg cttatattca cttaagcagc atcagattta
     2881 tacccgataa tcaataccgg ttttcattat tcggtattac gctccggtaa attcaattaa
     2941 atactgtctc atattttata cccatacaca ttgcctgtat tattgctgcc aaatacaata
     3001 ttgccgccat acgctattat tatttcgaat gaacaccgat aaataaacac cggcataaaa
     3061 ggcctgacgg cataaaaaaa gctctgcagg actgcctcgc aaagcggttt ttacttatgt
     3121 attgacggca ccggaatata tgatcgacag gtcggtccag ggcatcatcg tacacggagc
     3181 cggctgcccg tatcgccgcc ccggtcagcc taatcctgtt cttttgtccg ttctgccttg
     3241 tccagcgtgt ttctcacttc attcagttcc gcttcccttt ttgccagttc aagcttcagt
     3301 tccgtatttt gattgccaag tattgtattc tcctgctgca gtgccttatt ttccctcttc
     3361 agtttctcca gttcctcggt ggctttctta agctgatttt ttagttcctg ttcagaatcc
     3421 cggctcttga acaggtcatc gaccacattg attgccgtca gaacagcagc catcgaagtg
     3481 ctgagcttgt tgtttatttt aagtatatcc gtcatctttt tgtcaacata aaggccaacc
     3541 ctgtgtatgt attcctccgg ttccgatcct actatcgtat aatccttgcc ggcaatccta
     3601 agttcgacct tgtttttggc agtcaatgaa atcagcatcc tttcggcttt ataaagaaat
     3661 tcctggcacc cgtcgaagaa aaattataag gagatcattt ccattaagtc agcgcatcgg
     3721 aaatgtctcc ttatattata caatatttcg ccaaaaaagc atatctttta atattttttg
     3781 actgtttatc cttgctgttc ttttgcctcg tcggcctgct ccgctttttg cggttcggca
     3841 tagttcctga gtatgttagt cagatctatc cctgtcaacc cttttatcgt ttcaggcaac
     3901 tgggccatga tctccgtgat atccctcgtt atcttcgaag ccccggaatc tccgatcatg
     3961 acgatctttt cagtcttcgc aagaggctgt gctatggctg cggctatctc aggcatcttc
     4021 tcgatgacca tttgcgcaat ggcggcttcg ttgaactgct tgtaagcttc agccttcttt
     4081 gcgagcgctt cagcctcggc ctcgcccttt gccttgatta cttccgcctc cgcaagacct
     4141 gcggctttga tagcttccgc ctttgcggca ccttcagcct ttattgcgaa tgcctgtgcc
     4201 tcggcttcca tcgacttttt gaccttatcg gcttcagcgc tcagctccgt cctgtacttt
     4261 tcggcttcag caggcttcct gatagttgcg tccagttcct gtgcctttct gagcacttcc
     4321 tgctcctgca gttcgatctc tttctgcttc tttacgatct cgacctgcat ggcttccttt
     4381 tcgacttcct gctggagcgt gtactttttc ttgtcgtatg cgagatcggc ctcagccttc
     4441 ttttcattta tcgaagcctg atactcggcc attttcattt ctttgtccct gttggcttca
     4501 gcgactttgg tctcagccat gtatcttgcc gtctggcctg cctgcatagc ctcagcgctc
     4561 tttatgtcag cgtccctctt tgcttctgcc tggccgatca cggcatctct ctggactttt
     4621 gcgatctgcg ctttaccaag gctttcaagg taaccgttct tgtctgcgat ctctctaatc
     4681 gtaaacgaga ttatcttaag gcccatgttc gccagatcac tggctgccag ttcctgcacc
     4741 ttctgtgcga acgcgtccct gtttgccatg atctcttcga cggtgaggtt gccgacgatg
     4801 gaacgcagat ggccttcaag ggtctgcgtc gctatccgtc tgatctcttc ctccttcagg
     4861 ctcaggaact gctctgccgc agtcgcgata gcctcggtgg ttgaatcgat ctttacctgg
     4921 gccactccgt cgacactgac ctgtactccc tgcgacgtat atgcgctgga gttcaggtcc
     4981 aggggcatga tcctcagaga tatggttttg gccttctcaa ggatcggcca tacgaatgtg
     5041 gctccgccct taacgatcct gaacccgacg acctggccgt tcggaagcct ctttttgcgt
     5101 cctgacacga tcagcgcttc atccgggcct accttgacat atcttcccac gaataccgat
     5161 atcagtatga agatgacaac gacaagggca annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nacatatctt cccacgaata ccgatatcag tatgaagatg acaacgacaa
     5341 gggcaatggc tatcagcact ggtataaggt atcccatctc aaatcctcct ttatatcgtt
     5401 ttttttattt catgcatcgc tgcatgtttt tcccttctgt tcccggtgtg cggaaacaac
     5461 gcatgcattc ggcagggtct gcccgcatgc ctcagttccg gtttttcgtt tccgttatgt
     5521 cttcctttcg tttcagtact ccttcttcca actctatgtc atcgatggtc agcttctgct
     5581 gtacgactgc tgcgttgcct gcgatctccc ttatgatgac gggtgtcccc cttcggatct
     5641 cgcagccttc cagcgacctg gcggcaagag tgtatctgat gccttttatc gtataggcta
     5701 tctcacccag cccttttgcc ggaacagtag ttatgacatc tgcttcggca ccgacaaggt
     5761 catcgagtga aaatagcgaa ttggactggg agccgtacat aaacttgacg atgccgaaaa
     5821 atatcgctgc acctattgcg ccggcaaaac ccagcgccac aaatgtactg gccaggccgc
     5881 tcagtccgaa gcctttcata gatatgatcc cgaccgctcc aaatgtggaa atggcgcttg
     5941 ccataaccac cgggctgaaa ggcgaaggcg aacctgtcgt gtctgaagca tcgtgatgat
     6001 gagcctctgc tgcttgtccc gtttctgctc cgtgggcagc atctatcgca tgccctgcgc
     6061 ctatatccat gtcagcgtca ggtcctgctg cgtcaaaccc atccgcatca gcaccgatgc
     6121 ctgaaccgat atctgcgtcg ccgacgtcta cgctgccgat atctgcatcg ccgatatcaa
     6181 caccgccggc atccatgccg ccgatatcgg catcatgccc tatatctatg ccgtcgctgc
     6241 cggaatctcc atgcccgcca agtatgaacg acatggccgc gtagaaagct ccgaatgcaa
     6301 agcatccgat aaatacataa agcagagtat cacccccgtt tcatgtgctc cagattattt
     6361 taccatactt agccgagcat tacattgcca taacatccac acatgttacg cggcaaacag
     6421 aaaaaatgtt tatccggtcc cgggaaccgg accggataat ttacatgaat agatttacgg
     6481 atttttcagg gtggtttggt tttgaccgga taattcaatt accttcaaag ccggatatca
     6541 gggtactgat atcagagagt ccgcaggtcg ttcagcctat tgtccggaag tgcccggtca
     6601 cctgtatggg tgcagtacct tgccgtcaga cgggtcaata taaacgagcc agtatccgaa
     6661 cgctccgtac ctgggccggg tgccctgtgc ctgcacccag gtattcattt ctccgaaagg
     6721 tttcatgtct tccgcatatt tgccggggac tccgcagacc acatatttcc tgccgttttt
     6781 gtgccggaat atccctatta tcaggtgctt gtacctgtaa tgggccaaca tcacggcagg
     6841 gttgaacatc agcggggacc gtatattgtt ctggtacaat atattgttca ggtttacagg
     6901 gttcgtgacc ttccaccatt tatagtcgct cctcctgctg cggaacgggt cgcacacttc
     6961 aaagttcaag tcaagatcgt attccagact gtctatatcg gcgggagggt tggcctttgc
     7021 agacgccctg ccctggttta tccggcatga accgcatgga tcgggatctt gccggttgtt
     7081 gaccagtgca tcgcagatat tgccgttgag ataaacgcag tcggtgttta ctttatgcag
     7141 gttcggtgga gagacttcat ccgtccttcc tcccgtctgt ccatgtgaag tatctttgat
     7201 atcttcggtt ccggaaatcc cgtctcccgg cctgacgccg ggtccaggcc gcccgccggc
     7261 cgcgggctgc accggttcgg gtttctgctc ctgttttggt tctgcttccc gtcccggtcc
     7321 atgatcctgt ttttgttcct gccccggtcc gatttcaggt cctgcttccg gttccgattt
     7381 cagttcctgt ccaggttttt gttcctgctc ctgtacatgt tcctgttttt gttccaatac
     7441 aggttcagtc tccggttccg gttcccgctc atgtccaggt cccggcccaa attcaggtcc
     7501 ggatcctgtt tccggtcctg cttccttccc gggccgggcc agggttccag acccgtcagc
     7561 ggcaaacggc ttttgatccg gcagggcata tggttccctt ccggctgatc catccgtcaa
     7621 atgctcctgc tgtccgctga aatccaacgg ctgcaatgaa tcggccggct cgttctgata
     7681 acgcggatcc gcctgcccgc taaatggata tgacaccggt ttcttctgtc cggagtcctg
     7741 atgagatgct gcttcggttt tcccggtcgc tgtgtattct gccggcccgg gatgcacggt
     7801 gcccgcattc cgcaaatgga aaagcccact gtccgttttc tgtattccgg ccgtcttatc
     7861 tgcagcctga agcgctttcc tgagtccgtt tctccatccc gctgctttcc gcctgtacgc
     7921 agccaggggg catatcacat tattcgcagg accgtcccca tactccacca caaccgcggc
     7981 gatgtcatac tgatcgatgg gataaggtaa gccgcctacg tacatgggat cgaacccgca
     8041 tctgaactcg gcgcggccgg ccgtatgcgt tatatcgcca agcctgacat aatctgtcct
     8101 gtcgccggac tgccttacca ggtacagcgc atacaggaat ttcccttttc cttccttcag
     8161 gttatgtatg actgtttcga gtttcccctt tccttcccgg atctcaagct tgatatagcc
     8221 tgaaggtttc cctccttctt cgaagccgct gtcttcattg acaaatatca tgaaatttct
     8281 ttggaacctg ttcgtataag aagtatgcat atgaaccctc ccaccgtccg gctgctgtta
     8341 ttcatattga caaacctttg cctcatatac tatgtatatg taccgtcccc caaaaaaatc
     8401 acacgggccg gcgccacaag gcccgtgtct tttgctgtcc tggcttttgg ccggtacccg
     8461 ctacagcgcg gctttcggct tatgaaaaaa tgcaggaaca agttctcgaa attgcaaaaa
     8521 tttaatacga gtgataattc ccggcccaga ctgcacagaa gataccacag cgcatcatcc
     8581 ttttcatttc aggcagtttt tgcagcccga cggcgctatc ggcgccgtat tgatcctgtt
     8641 aagatttttg aatgtgttta tattgaaagt ttcataatta tgtgctaata tgatgaagat
     8701 ggttagtgaa aaaattaacc aagtctgcat gtgtatttac tcgtgttttg gctgattata
     8761 accttgttag caacaaatcc gttcagggaa ataatgaata tcacgtcacg atgaaaggag
     8821 agttaccatg acgaagaaca agaagctgct ggattgggtt aaggaaatgg ctgaaatgtg
     8881 tcagcccgac aagatttact ggtgcgatgg ttctgaggaa gagaataagc gtttgctgca
     8941 ggagatggtc gatgcaggta tggcgatccc gctgaatccg gaaaagaggc ccggatgcta
     9001 tcttttccgc agccatccgt ccgatgtggc acgtgttgag gatagaacct ttatttcatc
     9061 caaaacccag gacgatgcag gtccgacaaa caactggatc gaccccgttg aactgaagaa
     9121 aacgatgaaa gaactttaca ccggctgcat gaagggaagg acaatgtatg tcgtcccatt
     9181 ctcgatgggc ccgatcggtt cgcccatttc aaagataggt atcgagctta cagacagccc
     9241 ttacgttgta gtcaacatga ggatcatgac aaggatgggc aagcatgttc tggaagcgct
     9301 cggagataac ggagatttcg ttccgtgcct gcattcagtc ggcgctccgc tggctgaagg
     9361 ccagaaagat gctgcatggc cctgcgcccc gatggaaaag aagtacatca gccacttccc
     9421 cgaggagagg acgatctggt cctatggatc aggttacggc ggaaatgccc tgcttggaaa
     9481 gaaatgcttc gcactccgta ttgcatcggt ccaggcgcgc gacgagggtt ggatggctga
     9541 acacatgctc atcctgaaga tcaccgatcc caagggcaga aagttctatg tgacgggcgc
     9601 attcccgagc gcatgcggaa agaccaacct ggcgatgctc atcccgacga tccccggctg
     9661 gaaagtcgag accatcggcg acgatatcgc ctggatgagg ttcggggaag acggcagact
     9721 ctatgccata aatcctgaag ccgggttctt cggagttgct cccggtacat ccatggagtc
     9781 gaatccgaac gcaatgctca gctgcgcaaa gaataccata ttcacgaatg tcgcattgac
     9841 cgacgacggc gacatatggt gggaaggcat aggtactgat gctccggatc acctgatcga
     9901 ctggaaagga aacgactgga cgccagcttc cggtgaaaag gctgcgcatc ccaacgcaag
     9961 gttcacagcg cctgcaagcc agtgccctgt aatcgctcct gagtgggagg atcccaatgg
    10021 cgtgccgata tccgcaatac ttgtcggagg acgccgtccg agcacagttc cactggtaca
    10081 tgagagcttt gactggaacc acggtgtatt catgggttcc ataatgggtt cagaaataac
    10141 tgccgcaacc atatccgcaa atatagggaa agtgcgccgc gacccgttcg ctatgctgcc
    10201 tttcatcggc taccatgtag gcgattacct gcagcactgg cttgatatgg gtaaaaagac
    10261 gactcccgac aagctcccga agatcttcta tgtaaactgg ttccgcaagg ataaggacgg
    10321 caatttcctg tggcccggat tcggcgacaa cagccgtgtc ctcaagtgga tagtggagag
    10381 agtatccggc gaaggaaagg cagtcgagac tcctatcggt tacatgccca cagttgacgc
    10441 catcgatacc acaggcctcg gcatttcaga ggagaccatg aaagaactgc tgaaggtcga
    10501 caggaacgaa tggctcaaag aagtggaatc gatcagggaa cactatgcat catacggtcc
    10561 gaaaatgccg aaggaactct acgctcagct cgatgccctg gaaaagaggc tgaaaggctg
    10621 agatacggta tacgcgtaat acacagtaat aaagcccggt ttgctttaac cgggctttta
    10681 ttgtaccagt gaagttgtat cagcggcgca gatcatatat ctcattggca agccgtgcga
    10741 actgctgtat cgacagagtc tctccccgct gcatttcttc tatgccggcc ttgtccagca
    10801 gcagccggac ctggtccttg ccaatgccga tatagccggc gttgctcaac gcattcagaa
    10861 gtgttttccg tctctgaccg aacgccgctt ttactacctt aaagaaaagg tccctgtcct
    10921 ttagctcaac cggcggcgtt ttgtacagat ccagcctgac gaccgtcgaa tcgacgcccg
    10981 gccggggtgt aaagcaatcc ggcggtacat ccgttatgac cgtcggcctg gaatagtacc
    11041 ctaccgatac cgagagggct ccatagtcct tgcctcccgg ttttgccatc atcctggccg
    11101 ccacttcctt ctgcaccatg ataaccatac actcggcttc aaggccgtct tcgagcagct
    11161 tcattatgac aggcgtcgtt atatagtacg gcaggttcgc gacgaccttc agcgaacgca
    11221 gcgtgccgtc gggagaagtc ataaacggcg ccagctcttt cttcaggtcc agcctgagta
    11281 tatcggcatt tattatatgg acattgcggt acccgatgag caggtcgtgc agcacctgca
    11341 ccagccgctt gtcgatctcc acggccacca cacagcccgc atcctcggcc agatatgatg
    11401 taaggctacc gaggccgggg cctatctcga gcactgtatc atttggcgta agttcggcag
    11461 cctctgctat cttttgcagc acccagccgt ctgtcaggaa attctggccc aatgacctgg
    11521 acggatgtat gttgagtttc ctgaagatgc ttttgatatc tgcttccatg ccttttcctt
    11581 tctgcggcta ctgatcattc agtatgtata ccctgacttt tttgacgccg aagcggtcca
    11641 catactcctg cgagtcataa tagaggtcga tcttgttgcc ttttatcgcg cttcccacgt
    11701 ctcctgctat gcagaatccg taatccggcg tatttttatc cagtatctca acatacagct
    11761 tggtatacag cggtattacc ttgggatcca ccgctattac gccctttctg gccttcattc
    11821 ccgtggcggt tatcccgaac aacggatggt ccggcgcttt gcccgtatcc ttgagtgatg
    11881 cggtataagc cgtggctttc atatccagga ctttgctgta ccgtaccact tcacctcttg
    11941 aagttctgaa attcagcacc gtgccatact cgattatcat gttcacagga ttctgcaata
    12001 tggtctcgct taccagctgc tttgtgacct gtaccccgtc ttcgcttacc accttgtata
    12061 ccttttggag tacgccttcc ctgccttcct ggtatacccg ctcggttccc tcatccatca
    12121 ttttgttcgg ccttctgtgc actacaaaag gtatttcttc cgtctcgctt acatagtcct
    12181 ccgataccct tatgatcctc agttccatac cgctcgtgat cctgtcttcc atggacgcgc
    12241 ctgcgagcct gtctttgctc cctggcttta cggggctgtc ttcgaacatc tcctccacgg
    12301 tatccctgta tgtcatgacc tctgtcctct tgttgtcggc tgtaatataa accggtactg
    12361 ctctttttat gtacaattca ttggattttg ccgtctgcag tttcgtatca gccgaagcac
    12421 tgacataatc atactctgct acttcgatgc cgagctgatc cagcacttct ccaaaggtat
    12481 ttttcattgt cctgaaggaa atgactctgc cattgtcatt tataacgaca tccttctcaa
    12541 gccaggagaa aacgccgtac cctgcggcgg acgacaggac cgcggcggcc gcaaacaaca
    12601 gcaggtgcct cttcagtgaa aaaaaccttt taaagcttct gacaagtgtc aacaaccaaa
    12661 aaaacctcct gtaaaaatat tgctaaagga ctgcaagcta ttttattcca atccggtttg
    12721 tttgtcaaat ttattaccat aatgttccta ctaacatgat tatacacata cttttgggga
    12781 tttataccag ggcaggcgat tgcgccggat atgacaaaag atgctgacga tgcggtggtc
    12841 tatgccgccg gctctgcata atgcaaaagg agctgtattc atacagctcc aggggtgcag
    12901 gccggcgaaa ataaagctgc ggacgatcag taatccatcc cgctcatcgg gaaatactgg
    12961 ctgcccgcag aaggcaaagc gcccatgccg taaggcggat acatattgct gcccggtatt
    13021 cccgccattg gtccgccggt tattgacggc actgatcctc ctccggtata cggcatcata
    13081 tcatcagcag ccggtcccgc cccgaaaggc attgcgccgt tcatcgcatt caatcccata
    13141 agatttgccc catatatcat cgggcattgc agattcatca ggtatggaca gcaaattatc
    13201 ggtatctgct gcatcatagg cataactcca taaggaccgg gctgtaccgg cattttgttc
    13261 gcaggagctc cgccttccgc agactgtgcc gccattttat cctgtaccgt ttgcgccggc
    13321 agcgtaccag gcattgcctg catcgacggc aaatcggata cggtctttga tgaaaggaca
    13381 tcggcagcct tttcaggcga tacgccggaa acagtttttt cgggcgatgt tttgggagtt
    13441 tccttaacag cggattcttt cggaagtgtc tgtgcaggcg acgtcccggg cgcagccttc
    13501 agatcctgct tggggccgac atacggcatc gtgcctccgg gcacagcttt ggaaatatcc
    13561 ttcatccttc tatcttcctt catataatga ttcctccggt ttgggtacat tcagtatcat
    13621 tatatgagtc agataatatt atgtgaggct ttttatgtcg attgggacac gatcagtaaa
    13681 tattgatgga agatatgatg aaaccctttt tttcttttga atagtccagc tttacatata
    13741 tctcgttttc gccttcggca aagcgggaaa cggattcgtc cgcattttcc gcgagcccga
    13801 cgttcagcgt tacatccaga tagatctcgc cgttgctgga acccctgtac ctgtccactc
    13861 ttacttccgt gatctctatt ttttgcacag tggattccat ctgctcgaca taggcgctga
    13921 ggcttatgtt cctgtctttc tgcgggaccc cctcaaactc gatggcgtac gcttcgtcaa
    13981 aactgccgct gttcagcgcc tcaacccacg aaacgaccgg cttggacagg aactgtatgt
    14041 ctgcattttc cttcagtatg tttataagat cgatctttgc cctgagttcg gcttcttcct
    14101 ggagcttttc gccagccatc tgctcttttt ccttctgcag cctgtcgatt tcagccttgt
    14161 actgctcgat ctgattgccc aggcttttta cttcgtcttc aagtgcgcct atttccctct
    14221 tttggacgct tatgctggca ctgtagcttg cattgacgga ttcgagattc ttaatctccg
    14281 ccagcttgct ttccctgtcc cacaacaggt aattgaacgc gataaataca gcgatcagaa
    14341 gggcaaatat cattacgagg aaaaactttt tcatatcaca ccaccggatc gatttttatg
    14401 ggatgccgaa cagtctcttc gcattggctg tagtggtttc acagatgtac tcaaaaggtt
    14461 tccctttgat tttggctatt ttttctgcca caaacttcac gtacgcaggt tcgttcctcc
    14521 tgcccctgtg tggttccggg gccagatacg gtgaatccgt ctcgatcagc agcatatcgt
    14581 cgggaacata ccttatcacg tccggcagtt tccttgcgtt tctgaacgtg gccggcccgg
    14641 ctatagaaat catgaatcca agttccatga cctctctggc catttcaacg ctgccgctga
    14701 aacaatgcag gacgccgccc acatcccttg cgccctcgct tttgagtata ttgagcgtat
    14761 cctcatgggc gtccctgtca tggacgataa caggcagctt cagttcccct gcaagcgata
    14821 tctgttttgc aaaccaggtc ttttg
//
LOCUS       DAOSCC010000030        14788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2754_length_14788_cov_4.616643, whole genome shotgun sequence.
ACCESSION   DAOSCC010000030 DAOSCC010000000
VERSION     DAOSCC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2754_length_14788_cov_4.616643"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLG72_02980"
     CDS             complement(<1..1533)
                     /locus_tag="PLG72_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ79796.1"
                     /translation="MLFGVLVFIICSGLGLASYITALNSVLDVLEETLPKAAVEASAT
                     IENATQNQLNKLNIIASLDFMKVLKNGNTGDPALKAFMSDEISRSGHKLLILADKNGK
                     VLYSNGTVTDVKNNPFFESALSGKDTVSEPMPDKNGSGIVMVYAVPVKVDGETAGVLM
                     AYRDGLELSEFAGRVKYGESGQAYIINKQGRTIAHADKNIILQILEASAANADQSSAS
                     DGNGSDAVSSAATGAQSSDAVSSATTSAETADAASSATAPAGGENTGVQLRARESVSA
                     QVGFDGFFEIQKQMTEGKTGFGEYVYNGVPKFTGFAPVDKYGWSIGIAVDRDEVLSGL
                     SSLRLTVLAISVIFLAAGLAIAYILGKGISTPITYLSEECRIMSGGDFSKAMADKYTM
                     RRDEIGELARAFNNINVNVSGIISNVVGEAGSVGEAIANINENMAALTSEINLMSGII
                     NKLSQKMDENSASAEEMNATTEEIEDAVDSIANDSMQSAETAGEVSRRAEKLKATALD
                     SQK"
     misc_binding    1856..2157
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2557..4539
                     /locus_tag="PLG72_02985"
     CDS             2557..4539
                     /locus_tag="PLG72_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ79797.1"
                     /translation="MHANRPVFPKRAVITAGMPYGNKELHLGHIGGVFVHADVFARFM
                     RDRIGKGNVIFVSGTDCYGSPIVENYRQLVESGSFDGTIEEFVRSNHEKQKEVLDEYR
                     ISVDLFAASGIGRAAEIHREYSAEFIRRLYDNGHLIKMTTAQFFDAEAGVFLNGRQVV
                     GQCPVAGCASEKGYADECSLGHQYMPVELINPRSTITGKKPEMRDVTNWYFRLDNFRE
                     LLVQWVEKLEKDPASRKFVIKSIREFLEPPVIYVKKDYTGQLEAIRQLFPDFELVDEE
                     GKSSAVMVFKELDSREMACSVLTEHGIRYRTGKTIVPFRLTGNIGWGVPAPCIEGLDG
                     LTVWVWPESLWAPISFTMTYLEMTGKDREDWKKWWCTGESKVYQFLGEDNVYFYGPAE
                     MAMFMGQQGKNPASGPPEGELQLPELIVNNHILFLNKKASSSGEIKPPTARDLLNYYT
                     AEQLRAHFFGLGLGIRSVGFQPKPLNPAAAERDSDPVLKEGNLLSNVFNRAVRSCFYT
                     AQKYSGGKIPVREISSRVLEDARDTVLEYEWLMYRHEFHLVMNLMDTYIRNINKYWTA
                     KMKEADDTGNDELRLQVLADAFHMVRTAAVLMHPIAPEGTEMIREYLGLGEEFWSWDR
                     IFDTVYSFMEDPGEHKLKFLEPRVDFFKKHPSQIAS"
     assembly_gap    4562..4661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4662..6487)
                     /locus_tag="PLG72_02990"
     CDS             complement(<4662..6487)
                     /locus_tag="PLG72_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ79798.1"
                     /translation="MHDTGKTPNTKMRSFPYSDVELLPGQLSRKTESNRRYLLSLDTE
                     NLLRNHYIEAGIWQSNQLPDNIHGGWESPTCQLRGHFLGHWLSAAARVYAATGDAEVK
                     GKADRIVSELARCQKENGGEWAGSIPEKYLEWIARGKSVWAPQYTIHKTFMGLMDMYK
                     LAGNRQALEIADAWSNWFYRWSGKFTREQFDDILDYETGGMLEIWADLYGITDRKEHY
                     ELMQRYYRRRLFDPLLAGEDVLTNMHANTTIPEVLGAARAWEVTGEQRWRDIVEAYWQ
                     LAVNKRGYFCTGGQTCGEIWTPPYEMSARLGEMNQEHCTVYNMMRLAGFLLKWTGDPV
                     YADYWERNLYNGIMAQGHWEGFYPSGVKPQHPLTGLIAYFLPLHEGAEKHWGSHTHDF
                     WCCHGTLVQANAAHTEDIYFEDPDGVAVCQYIPSALRWDRNGSMIRIQQHINHRAGSC
                     NIVNKTNIEYMHRPMSLAVDFTVECDSPAQFEMKFRIPWWIMGDVKIYVNGELQNIQC
                     KPSSFCTISRTWSKDTVNIELPKGLTACPLPDDPETFAFMEGPVVLAGLCDEGRVLYG
                     DAEHPETILVPDDERKWSSWNTGYRTKGQDRNIRFIPLYDVG"
     gene            7047..7922
                     /locus_tag="PLG72_02995"
     CDS             7047..7922
                     /locus_tag="PLG72_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79799.1"
                     /translation="MGSEILLGLKDIVKVRIVDFCRADRPFVHADRILDFNVFIYIVS
                     GEMRIWECGTEYVIGERDAFFLKKGLHHYGKHEIPAGTTWYYIHFHGGADDGNVRQLS
                     GYRPVPASGEFTEEDYDQYIRLPKLLKVDNPKLIERKLDALTQMCKSSNDLKMAYLSY
                     GTMELFFDLFNQERSRSELDRGDIITRKIIMFLESNISRKLSSGEISDYMCMNYNYIS
                     GVFREKTGMSIMEYHGKIRINEAARLLRNSNMNVSEVSGRLGFEDPLYFSRVFKKVMG
                     YSPSDYIKQAYFRLR"
     gene            complement(8442..10019)
                     /gene="gpmI"
                     /locus_tag="PLG72_03000"
     CDS             complement(8442..10019)
                     /gene="gpmI"
                     /locus_tag="PLG72_03000"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ79800.1"
                     /translation="MNKKVVLVVMDGIGKGRGGYGDAVSMANTPTLDMLLQNYPNTYI
                     KAHGTAVGLPTDNDMGNSEVGHNALGCGQIYSQGAKLVNESIESGEIYRSQSWHEIVS
                     NCIKSDSTLHFIGLLSDGNVHSNINHLKSMIARAKEDGVKKIRVHALLDGRDVPALSA
                     TTYINDLEAFLAELNGDSFDAMIASGGGRMVVTMDRYQADWDMVRRGWETHVLGEGRR
                     FASATEAIMTYREELNVTDQYLPPFIIEKDGQPAGRIVDGDSVVLFNFRGDRAIEISM
                     AFEYEDFSKFGRRYMPKVVFAGMLQYDGDLLLPKRYLVSPPNIKNTLSELLVKNGIRQ
                     FAVSETQKYGHMTYFWNGNRSGKFSEELETYCEVPSDIVPFDERPWMKSAEITDKVIE
                     AIKSGEYGFIRCNYPNGDMVGHTGNLDAAVIAVEAVDLGLARILKAAEEHDAALIVTA
                     DHGNADDMILTDLSGKAQPKTAHSLNPVPFIIYDGHKTYRLRQGNFGLANVAPTIVQI
                     LGLDIPVMWEESMLDIY"
     gene            complement(10245..10949)
                     /gene="sfsA"
                     /locus_tag="PLG72_03005"
     CDS             complement(10245..10949)
                     /gene="sfsA"
                     /locus_tag="PLG72_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOJ79801.1"
                     /translation="MKYDKIIEGKFISRSNRFVAYVSVGGMKTKAHVKNTGRLKELLV
                     DGATVYLQKHDDPKRNTALSLIAVKKGDQIVNIDSQAPNKAMHEWLKGSPEIIIGTDE
                     KISAIRPEYGYRDSRFDFLIETDKRKILMEVKGVTLEEKGTALFPDAPTERGVRHITE
                     LCGSMRDGYYPVIAFVIQMDGVRQFSPNAKTHKEFADALVSAHEQGVCIIALDCEVTR
                     GSMVIRDFVDIVLQQT"
     gene            11319..12854
                     /locus_tag="PLG72_03010"
     CDS             11319..12854
                     /locus_tag="PLG72_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOJ79802.1"
                     /translation="MDTKTVGAKFGWNFDNTYARLPETFFSFVGPTPVRSPEVAVLNH
                     DLARSLGLDPDALSGSEGAEIFAGNRIPEGAQPIAQAYAGHQFGYFTMLGDGRAILLG
                     EHISPSGMRYDIQLKGAGRTPYSRGGDGRAALGPMLREYIISEAMYALGIPTTRSLAV
                     ATTGEKVYRETALPGAVLARVAASHIRVGMFEYAARWRTVEELKALADYTIRRHFSCP
                     EDPKCCGGSDRNGDMPGHIHRRNENPYLCLLKEAVRRQAELVAKWQLVGFIHGVMNTD
                     NMALSGETIDYGPCAFMDAYDPDTVFSSIDIHGRYAYGRQPGIAAWNLARFAETLLPL
                     LHEDENKAVELARGAVSGFGELFGATWLRGMRSKLGIFDEESRDADLINSLLEIMYKN
                     KADFTNTFRALTYNEDLDIGMFSTDEFRGWRARWHERLGRQRHVESAVFRLMRESNPV
                     IIPRNHRVEEALGAAEKRGDYSAMERLVRALSKPCENTPDKDYFTAPPGPSFCGYRTF
                     CGT"
     gene            13012..13995
                     /locus_tag="PLG72_03015"
     CDS             13012..13995
                     /locus_tag="PLG72_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ79803.1"
                     /translation="MQLLKKMMYRRIFPVVLSISASIFSMLVTLRWNRQLSSVINEIN
                     AAAHIPTDAIITAAAAMLVMAASNYVLGLSSAWACECMAHDLRMGFAKHFATLSLAEI
                     ENINAGEQMSKLQNEISEVVGFLNADLFTFIDDVIRFIGYFTWLLLLDPKLTLISFIP
                     TAIQMWYASYASRAISEAAQQSQHANGRLNGFAESLVAVFPVMKLFDAVKLVRSKYDA
                     ALEEWERAAVRQERRRAALMSPSGLMSCIPLLLLFAVGGMQVVRGELSTGGLYIFINL
                     SGIVSGVMMNMPGRIASFRRFAVNLRRIGPYMTAGFAAGKADGGSGVSAGV"
     gene            14147..14785
                     /locus_tag="PLG72_03020"
     CDS             14147..14785
                     /locus_tag="PLG72_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ79804.1"
                     /translation="MSIRAENLWFTYNDAPVLRGIDLFADTGEHIGITGMSGCGKSTL
                     LKLMAGLYRAQDGAVEVHGARRPEDIRKNVAMVMQNAVLFPASIRENITCGHQMSEEN
                     LRQVCEAAQLTEWIASLPDGIDSFVGERGGKVSGGQAQRIAIARAIAKNAPVILLDEA
                     TSALDSDTGSAVLAALESLTRGRTVVSVSHRYETLAGCSRIYKLDGGRLYEL"
BASE COUNT         3401 a         3619 c         3995 g         3673 t
ORIGIN      
        1 cttctgtgaa tcaagggcgg tggccttgag cttctccgcc cgcctgctta cttcgcctgc
       61 ggtttcggcg ctttgcatgg aatcgttggc tatggagtcg acggcgtctt cgatttcttc
      121 ggttgtagcg ttcatttctt ccgctgaggc agagttttca tccatcttct gcgacagctt
      181 gtttatgata ccggacatga ggtttatctc ggaagtcagc gccgccatgt tttcattgat
      241 gttcgcgatg gcttcgccga cgctgccggc ctccccgacg acattgctga ttatccctga
      301 aacgttgaca tttatgttgt tgaaggccct ggcaagttcg cctatttcgt cacgcctcat
      361 ggtatattta tccgccatgg ctttcgaaaa atcaccgccg gacataatgc ggcactcttc
      421 cgacagataa gtgatgggcg ttgatatgcc ttttccaaga atatatgcga tcgccagccc
      481 tgccgccagg aatatgacgg atatggccaa aaccgtcagc ctcagtgatg acagtccgga
      541 cagcacttcg tccctgtcca ccgcgatccc gatcgaccag ccgtacttat ctacgggtgc
      601 gaatccggtg aattttggta caccgttgta tacgtattcg ccgaaacctg ttttgccttc
      661 cgtcatctgt ttctgtattt caaagaaccc atcaaagccg acctgtgcgg acacgctttc
      721 cctggcgcgc agctgcacgc cggtgttttc tccgcctgcc ggggcagttg cagatgaagc
      781 agcatcagcg gtttctgcgc tggttgttgc agaggaaacg gcatcagagg attgagcgcc
      841 tgtcgctgcc gaggataccg catctgatcc atttccatcg gaagcggagg attggtctgc
      901 attggcagcg gaagcttcca gtatttgcag gatgatgttt ttgtcggcat gggcgatcgt
      961 ccgtccctgc ttgtttatga tataggcttg tccgctttct ccgtatttga ccctgccggc
     1021 aaattcgctc aactccaggc cgtccctgta agccatgagt acgccggctg tctcgccatc
     1081 caccttcaca gggaccgcat acaccatgac aatgccggat ccgttcttgt caggcattgg
     1141 ttccgagact gtgtccttcc cggatagtgc gctttcaaag aagggattgt ttttaacgtc
     1201 cgtgacagtg ccgttgctgt ataaaacctt tccgtttttg tcggccagta tcaacagctt
     1261 atggcccgaa cggctgatct catcagacat aaaggctttc agcgcaggat cgcctgtgtt
     1321 gccgtttttc agtactttca tgaaatccag cgaagcgatg atgttcagct tattaagctg
     1381 gttctgcgtt gcgttttcga ttgtcgccga cgcttctaca gcggctttcg gcaaggtttc
     1441 ttcgagaaca tccaggaccg aatttaaggc tgtaatgtat gatgccagac ccagaccgga
     1501 acaaataatg aatacgagaa caccgaataa cacaataatc cttgtactga tcctccgcat
     1561 atacccaagc tccatttttt tgaatatttt cacagaatat aaattcgtag aatatatacc
     1621 cagtttactg ctgcgactat catgtgcttt ataaaatatt tatatcagga aatgggtttc
     1681 ctggcaacaa aaaatggtta cccggaatac tgctgattga atggcctgca gttcctgata
     1741 ccggacacca ccgggaaaat gcagtttttt acaacgccgt gggataataa tcaggcttta
     1801 caataccggc ataaatacat ataatatttg aaaacaagct gaaacgaaaa cagacatgaa
     1861 tgcgatgaca agggatagta tgccggcgct tactttcaca gagaacccgg gttggtggga
     1921 gcggggactg gagaccggtt gaacaggcct tggagcactg caccgaacag gcggcatatg
     1981 atactttgcc caagtaggct gcggcggatg cacccgttac agtgccgggg aatgatatgt
     2041 gaaattgact ttccgttatg ttagccggtc ttttcgcatt cgtccctgtt gaggctgatg
     2101 ccgcaaggta tcggtaaacc ggggtggcac cgcggagtta aggctctcgt ccccaggatc
     2161 gtatcctgga ggcgggggct tttttgatgt gttccgcagg attgacggca gacgggttca
     2221 gccggatgat tcacccaaat gtttttgcaa aattgctcag ccagccgcca tgttattccg
     2281 acgtgtttcc cgtatccctt gctccgtctg cgtttcagcc ataaaccgat cagatactgc
     2341 accgtgcatg gtacgtgacg tatttggcac agcggttgtc ggcagactta aaatctgacg
     2401 taacgtgtat aagcatacag aaaagcctat ccggcataac aggcgccgac gaacggagca
     2461 ggaatttaaa gagaaggaat acatatatag aaaaacaagg gaggagatca ttttggggca
     2521 gaattgattc aaagcaaaac gggcactttt tccgatgtgc acgcaaaccg gccggtgttt
     2581 ccaaaacggg ccgttatcac ggcagggatg ccgtatggca acaaggaact tcaccttggc
     2641 catattggcg gggttttcgt ccatgccgat gtgtttgcgc ggtttatgcg cgatcgcata
     2701 ggcaagggaa acgtgatctt tgtttccggg acggactgct acggttcgcc gatagtggag
     2761 aactaccgcc agctggtgga aagcggctca tttgacggta ccatcgaaga atttgtccgg
     2821 agcaaccatg agaagcagaa ggaagtactg gatgagtacc gcataagcgt ggaccttttt
     2881 gcagcatcgg gcatcggacg ggcagcggag atacacaggg aatactctgc cgaattcatc
     2941 agaaggttat acgataacgg gcatctcatt aaaatgacca cggcccagtt cttcgatgca
     3001 gaagcgggcg tattcctgaa cggacgacag gtcgtggggc agtgccctgt tgccggctgt
     3061 gcgtctgaaa aaggatatgc cgacgagtgt tcgctcggcc accagtacat gcccgtcgaa
     3121 cttataaatc ccaggagcac catcacgggc aaaaaacctg aaatgcggga tgtgacgaac
     3181 tggtacttca ggcttgacaa cttccgtgag ctgctggtgc aatgggttga aaagctggag
     3241 aaagaccctg cttcaaggaa gtttgttata aagagcatcc gggagttcct ggagccgccg
     3301 gttatatacg tgaagaagga ttataccggc cagttggaag ccatcaggca attgtttccg
     3361 gattttgaac tggtcgacga agagggtaaa tcttctgcgg tcatggtctt taaagagctt
     3421 gatagcaggg aaatggcctg ctcggtgctc actgaacacg ggatcaggta ccgcacgggc
     3481 aagacgattg tgccgttcag gctgaccggg aatatagggt ggggagtacc cgcgccgtgt
     3541 atcgaagggc tggacggcct gactgtatgg gtttggcccg agtccctctg ggcgcccata
     3601 tctttcacga tgacttacct cgagatgacc gggaaagaca gggaggactg gaagaaatgg
     3661 tggtgtaccg gcgagtcgaa ggtgtaccag ttcctaggag aggataacgt gtacttctac
     3721 gggcctgcag aaatggcgat gttcatgggg cagcagggga aaaatcccgc atccggtccg
     3781 cctgaaggcg aactccagct gccggagctt attgtgaaca accacatcct tttcctgaac
     3841 aagaaggcca gcagcagcgg agagataaag ccgcctacgg cgagggacct gctcaattac
     3901 tataccgccg aacaactcag agcgcatttc ttcggccttg gcctggggat caggagcgtc
     3961 gggttccagc ctaagccgct gaatcctgcg gctgccgaaa gagacagcga tcctgtgctc
     4021 aaggaaggga acctgctgtc caatgttttc aaccgtgctg taagatcatg tttttatacc
     4081 gcccagaagt acagcggcgg gaagatcccg gtcagggaaa tcagcagccg ggtcctggaa
     4141 gatgcccggg acaccgttct cgagtatgag tggctgatgt accggcatga attccatctc
     4201 gtaatgaacc tgatggacac atacatccgc aatatcaaca agtactggac tgcaaagatg
     4261 aaggaagcgg atgataccgg caatgacgaa ctccggctgc aggtgctggc ggatgcgttc
     4321 catatggtgc ggacggctgc cgtactcatg cacccgatag cgcccgaagg cacggaaatg
     4381 atacgggaat acctggggct tggggaagag ttctggagct gggacaggat attcgatacg
     4441 gtctattcgt tcatggaaga tcccggggag cataagctga aattcctcga acccagggtc
     4501 gacttcttca aaaagcatcc gagccagatc gccagttaac tgtttggcgg ctgtcaggca
     4561 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntagcccacg tcatacaacg
     4681 gtatgaagcg gatgttcctg tcctggcctt tcgtcctgta gcctgtgttc caactgctcc
     4741 actttctttc atcatccggg acgaggatgg tttcaggatg ttcagcgtcg ccataaagga
     4801 cgcggccttc gtcacacagg cctgccagaa ctaccggacc ttccatgaag gcgaaggttt
     4861 ccgggtcatc aggcagcggg catgcggtaa ggcctttggg cagttcgatg tttaccgtat
     4921 ctttgctcca tgtcctgctt attgtgcaaa agcttgacgg cttgcactgt atattctgta
     4981 gctctccatt gacgtaaatt tttacgtcgc ccattatcca ccagggtatc ctgaatttca
     5041 tttcaaactg tgccgggctg tcgcattcca ccgtaaagtc aacggccagc gacattggcc
     5101 tgtgcatgta ttcgatattt gttttgttga cgatgttgca gctgcctgca cggtggttta
     5161 tatgctgctg tattctgatc atgctgccgt tcctgtccca gcgcagggcc gaaggtatat
     5221 actggcatac cgccacacca tcgggatctt cgaagtaaat atcctcggtg tgggcagcat
     5281 tggcctgtac tagtgttcca tggcagcacc agaaatcatg cgtgtgggat ccccagtgtt
     5341 tttcagcgcc ttcgtgcaga ggcaggaagt aagctatcag accggtcaat ggatgctgtg
     5401 gtttgacacc gctcgggtaa aagccttccc aatgcccctg ggccattatt ccgttataca
     5461 ggttcctctc ccagtaatcg gcgtaaacgg gatctcctgt ccatttgagc aggaatcctg
     5521 caaggcgcat catgttgtag acggtgcagt gctcctggtt catttcaccg agcctggccg
     5581 acatttcgta cggaggcgtc cagatctcac cgcaggtctg tccgcctgtg cagaaatatc
     5641 cgcgcttgtt taccgcaagc tgccagtatg cctccacgat atcccgccag cgctgttcgc
     5701 ccgtaacctc ccatgcccgg gcagcgccca gcacttcggg gatcgtggtg ttcgcgtgca
     5761 tattggtgag cacgtcttcg cctgcaagca atggatcgaa caggcggcgg cggtagtatc
     5821 tctgcatcag ttcataatgt tccttccggt cggttattcc atacaggtcg gcccatattt
     5881 ccagcattcc gcctgtttca tagtccagga tatcgtcgaa ctgttcgcgg gtgaatttgc
     5941 ctgaccaccg gtagaaccag ttggaccatg cgtcggctat ttcaagcgcc tgcctgttcc
     6001 cggccagctt atacatgtcc ataaggccca tgaaggtttt atggatcgtg tattgtggcg
     6061 cccagacgga cttgcccctt gcgatccact ccaggtattt ttcagggatc gagccggccc
     6121 attcaccgcc gttttctttc tggcaccttg ccagttcgga cacgatcctg tcagcctttc
     6181 cttttacttc ggcatcaccg gtcgcggcgt atactcttgc cgccgctgaa agccaatgcc
     6241 cgaggaaatg gcctctcaac tggcatgtgg gcgactccca gccgccatgg atgttatccg
     6301 gcagttgatt ggactgccat atgcctgcct cgatgtagtg gttccgcagc aggttttcgg
     6361 tgtcaaggct caacaggtat ctacggttcg attcggtttt tctgcttagc tgccccggca
     6421 gcagttccac atcgctgtat gggaaacttc tcattttggt attgggcgtt ttgccagtat
     6481 catgcatttt gtataccctc cctgtaatcg tgtttttcct gcacgtcgat atcgaatatt
     6541 atcgatgata caggctgaaa tgcctgtatg gatgcatctg cgcaaactcg gatgcaacgc
     6601 ctggtgcaca tgactaaatt ccgcgagcaa atgcggccgt gtagattcgg atcctgtaaa
     6661 agcaaatccc gcataagcaa acagcgcaat taccctgaac aactttctgt acaacttaat
     6721 gatagttttc cgggacgctt ttgtgaataa aggaaacttc agaaaacatt cactattttc
     6781 atttgccgat cgtcatttca tactgacagt catttcacct gatttgcctg cttcggttta
     6841 tttactctcc atttgctcga cgaatctgca cgcatattgt atattattag cataaaggaa
     6901 atttatattt cggcaaaggt cgacttttgc ctgtttcaag gtttagcgtc agcctgtttg
     6961 aagtttcctg ctgccggcgg atggcttttg gaacagcgaa gatttttaag cggcaataac
     7021 ggatggacgg aggtcacggt gtcgttatgg ggtcggaaat actgctggga ctcaaggata
     7081 ttgtaaaagt caggatcgtg gatttctgca gggcggacag gccttttgtc catgccgaca
     7141 ggatactcga cttcaacgtc tttatttaca tcgtctcagg cgagatgagg atatgggaat
     7201 gcggcaccga gtacgttatt ggggagaggg atgccttttt cctgaagaaa gggctgcatc
     7261 actacgggaa gcatgagatc ccggcgggaa cgacgtggta ttatatccat ttccacggcg
     7321 gcgctgacga cggaaatgtc cggcagttga gcggatacag gcctgttccg gcttcgggcg
     7381 agtttaccga ggaggattat gaccagtata taaggctgcc gaaactcctg aaagtggata
     7441 atccgaaact cattgaaaga aagcttgacg cgcttaccca aatgtgcaaa tcgtcgaacg
     7501 accttaagat ggcatatctc agctatggta caatggaact gtttttcgac ctcttcaacc
     7561 aggaaaggag ccggtccgag cttgacaggg gcgacataat cacgcggaag atcataatgt
     7621 ttcttgagag caacatttcg agaaagctga gttccggtga gataagcgat tacatgtgca
     7681 tgaattacaa ctatatcagc ggcgtcttca gggagaagac aggaatgagc atcatggagt
     7741 accacgggaa aatcaggatc aacgaagccg ccaggctgct cagaaactcg aacatgaatg
     7801 tttccgaggt cagcggcagg cttgggtttg aagaccctct ctacttcagc cgcgtgttta
     7861 agaaagtcat gggctattcc ccgtcggatt atattaagca ggcatatttc aggttaagat
     7921 gaaaaagctt cgtccggcta aggtgaaata ttcctcgtca actgtgcaaa attttcgttt
     7981 atgggcagga aatcgcaatg gaatggttgc taaaatggct aattggttta cataacatgt
     8041 aattttcttt cattatcgcg gttttatatt gaaaaatatc catgtcggtt gcccagaagt
     8101 gaaaaaattg cacatccagg cccagaaaaa tgggtcagcc tgcccagaaa cgaaaaaact
     8161 gcacatccag gcccgattat atcaggagat atctggttaa taaggaggaa tctataagag
     8221 atcctgctta catgaagatc tcacgggaga tttcttaatg aaaggtggat caaagcgtaa
     8281 tacaggcccc acaggaggaa attccatgcc gtgccatgcc agaagccggt gagcgactaa
     8341 gaaggttcgg gcatgcctcg gcatgggccg ggcattcacc agcatgggct ggaaatgcct
     8401 cagcataggc agggcatacc tcaataaggg ctgggcatgc ctcaatatat gtcgagcatg
     8461 ctctcctccc acatcacggg gatgtcgaga cccagtatct gcacgatcgt cggcgccacg
     8521 ttggcgagcc cgaaattgcc ctgcctgagc ctgtatgttt tgtgcccgtc gtaaatgatg
     8581 aaaggcaccg ggttgagcga atgggccgtt ttcggctggg ctttgccgga caagtcggtc
     8641 aggatcatgt cgtcggcgtt gccgtggtcc gctgtcacta tcagtgccgc gtcatgctcc
     8701 tccgcagcct tcagtatcct tgccaggccg agatcgacgg cttccacggc gatgactgcc
     8761 gcatccaggt tcccggtgtg gccgaccatg tcgccgtttg gatagttgca gcggatgaat
     8821 ccgtattcgc cggattttat ggcttcgatc accttgtcgg ttatttccgc tgatttcatc
     8881 catggacgtt catcgaacgg aactatatcc gacgggacct cgcagtatgt ctcaagttct
     8941 tcgctgaatt tgccgctcct gttcccgttc cagaaatatg tcatgtggcc gtacttctgg
     9001 gtctcggata cggcaaactg ccttatgccg tttttcacaa gcaattcgga caaggtattt
     9061 tttatgtttg gcggactcac aaggtacctt ttcggcagga gcaagtcgcc gtcgtactgg
     9121 agcatgccgg cgaacacgac tttcggcatg tacctgcggc cgaacttgct gaaatcctcg
     9181 tactcaaacg ccatgctgat ttctatcgcc ctgtcgcccc tgaagttgaa cagcaccacg
     9241 ctgtccccgt caacgatcct gcccgcaggc tgtccatctt tttctattat gaatgggggc
     9301 aggtactggt cggtcacgtt cagttcttcc ctgtatgtca ttatggcttc agttgcagat
     9361 gcgaaccgcc tgccttcgcc cagcacatgg gtttcccagc cgcgccgcac catgtcccag
     9421 tctgcctggt acctgtccat cgtgactacc attctgccgc cgccggacgc tatcatggca
     9481 tcgaagctgt cgccgttgag ctccgccagg aacgcttcaa ggtcgttgat gtaagtcgtg
     9541 gcagacaggg cggggacgtc gcggccgtcc agcagggcat ggaccctgat ttttttcacc
     9601 ccgtcctctt ttgccctggc tatcatcgac ttgagatgat tgatgttcga gtggacgttt
     9661 ccatcggaca gaagcccgat gaagtgcagc gttgaatcgc tttttatgca attcgaaact
     9721 atttcgtgcc agctctgtga cctgtaaatt tcgccgcttt cgatgctttc attcacgagc
     9781 tttgctccct gggaatatat ctggccgcag ccgagagcgt tgtgcccgac ctcgctgtta
     9841 cccatgtcat tgtcggtggg caggccgacc gcggtaccgt gagcttttat atatgtgttc
     9901 ggatagttct gaagcagcat atcaagagta ggcgtgttgg ccatcgagac agcgtcgccg
     9961 taaccgcccc tgcctttccc gatgccgtcc ataactacca gcacaacttt tttgttcatg
    10021 acggatcctc cttttacatg tgaacttagc tttatctgca tatatacctg ataattgcca
    10081 ccgcagcagt tatgccttat cttttatcgg gcattatcgt ttcttcataa tcgtttttaa
    10141 ggagcatttt tccgggagca tcgccttaga tccggatatt gctttcggta actcactttg
    10201 ctgcgccaaa atacatgatt tgccgtttat agatcatgaa acattcaggt ctgctgcagc
    10261 actatgtcga caaaatccct gatcaccatt gagccccggg tcacttcgca atccagcgct
    10321 ataatgcata ctccctgttc atgcgcggag acaagggcat ctgcaaactc cttgtgggtc
    10381 tttgcattcg gtgagaattg cctgacgccg tccatctgta tcacgaaggc aataacgggg
    10441 taatacccgt ccctcatact gccgcacagt tccgtgatgt gcctgacgcc gcgctcggta
    10501 ggcgcgtcgg gaaacagcgc cgtgcctttc tcctccagcg tgaccccttt gacttccatc
    10561 agtatttttc ttttgtccgt ttctatgaga aagtcgaacc tggaatccct gtagccgtat
    10621 tcgggcctga tggcggatat tttttcatcc gttccgataa taatttccgg agatcctttc
    10681 agccactcat gcattgcctt gttcggagcc tgcgaatcta tgttgactat ctggtcccct
    10741 tttttcaccg cgataagcga taatgcggta ttgcgcttcg gatcgtcatg cttctggaga
    10801 tagacggtgg ctccgtcgac caggagttct ttgagcctgc cggtgttttt cacatgggct
    10861 tttgtcttca tgccgccaac acttacatat gcgacaaaac ggttagaccg tgatatgaac
    10921 ttgccttcaa tgattttatc gtatttcatg tgctgcctcc gcttatctta ttgtagcaca
    10981 agtgcatata atgcaggaac actgttgcgg aacacgccgg aacttgtgtt tagaggaata
    11041 ttaaggcatg aggaaacgca tatggcaaat cagggcatga gttttgcact gtatttataa
    11101 acccgtttcc gggtaacata atatagattt tataaaaaag ccagtcggga actgcagcgg
    11161 tgtcggcgac agccaacggt tttcagggat cagtgacggt ctcgggggcc agcagtggtt
    11221 taagggaccg gcaacggttt ctgatgtatg cctggcatgg caaataatgc cggaagagga
    11281 ctcgtataca aaactcgaat acagcagaat gaggaattat ggatacaaaa actgtcggag
    11341 caaaatttgg ctggaatttt gacaacacct atgcgcgatt gcctgaaaca ttcttttctt
    11401 ttgttggacc aacaccggtg cgttcaccgg aagtggcagt gctcaaccat gatttggcgc
    11461 gttcgctcgg actggatccg gatgcgcttt caggcagcga gggagcagag atatttgcgg
    11521 ggaacaggat cccggaaggc gcgcagccga tcgcccaggc gtatgcgggc caccagttcg
    11581 gatatttcac catgcttgga gacggacggg cgattttgct cggcgagcat atatcccctt
    11641 ccgggatgag atatgacata caactcaagg gcgccggcag gaccccctat tcgagaggcg
    11701 gggacggccg ggcggcgctt ggcccgatgc tgagggagta tataatcagt gaggctatgt
    11761 acgcacttgg gatacctacc acccgaagcc ttgccgtggc cactacggga gagaaggtct
    11821 accgggaaac ggctttgccg ggagccgtat tggccagggt ggctgcgagc catatcaggg
    11881 tcggtatgtt cgagtatgcc gccaggtggc gtacggtcga agaactgaag gcgctggcgg
    11941 attatacgat caggaggcac ttctcctgcc cggaggatcc gaaatgctgc ggcgggtctg
    12001 acagaaatgg ggacatgccg ggccatatcc acagaagaaa tgaaaacccg tatctctgcc
    12061 ttcttaaaga agctgtcagg cggcaggcgg aactggtcgc aaaatggcag ctggttggtt
    12121 tcatccatgg cgtgatgaac accgacaata tggctttgag cggcgagacc atcgattatg
    12181 ggccgtgcgc attcatggac gcctatgacc ctgatacggt gttcagttcc atcgatatac
    12241 atggccggta tgcatatggc agacagcctg gcattgctgc atggaacctg gcaaggtttg
    12301 ccgaaacact gctgccgctg ctgcacgaag acgaaaataa agctgtggag cttgcccggg
    12361 gcgctgtttc ggggtttgga gaattgttcg gcgccacatg gctcagaggg atgaggtcca
    12421 agcttggcat ttttgatgaa gagtcccggg atgcagatct catcaacagc ctgcttgaga
    12481 tcatgtataa gaacaaggcc gacttcacca ataccttccg ggcgctgaca tataacgagg
    12541 accttgatat aggcatgttc agcaccgatg agttcagggg atggagggcg agatggcatg
    12601 aaaggcttgg caggcagagg cacgtggaat cagccgtgtt ccggcttatg agggaaagca
    12661 atcccgtgat catcccccgt aaccaccgtg tggaggaagc ccttggagcc gctgagaaac
    12721 gtggggatta cagtgccatg gaaaggcttg tccgagcttt gtcaaagccg tgtgaaaata
    12781 cgcctgacaa ggattacttt acggcgccgc ccgggccgtc attctgcggt tatagaacgt
    12841 tctgcggcac atgaccggcc tttgaggcaa cagtgcagaa cacctgttga ctctgcccct
    12901 gggacatggt ttaaactgaa accgaggacg aaaaactagt gtgcggcgtg cttgccaacg
    12961 catctttggg gaaagtgttt ccattaagaa tgattttcag ggggcagagc catgcagttg
    13021 ctgaaaaaga tgatgtacag gcgcatcttt cctgtcgttt tatctatttc cgcgagcata
    13081 ttttccatgt tggtaacctt acggtggaac aggcaattaa gttctgttat aaacgaaata
    13141 aacgcagccg cccatatccc gacagatgca ataatcacgg cggctgcagc aatgcttgtc
    13201 atggcggcgt caaactatgt ccttggctta agttcggcct gggcatgcga gtgtatggca
    13261 cacgaccttc gcatgggatt tgcaaagcat tttgcaacgc tgtcattggc ggagatcgaa
    13321 aatataaatg caggagaaca gatgtcgaaa ctccagaatg agattagcga agttgtcggc
    13381 tttcttaatg ctgatttgtt cacgtttatc gatgatgtca taaggtttat cggctatttt
    13441 acatggctgc tgctgcttga cccgaagctg acgctcattt ccttcatacc aacagcgata
    13501 cagatgtggt atgcgtcata cgccagccgt gcgatcagtg aagctgcgca gcaaagccag
    13561 catgcaaacg ggcggctgaa cggatttgca gaatcgcttg ttgcggtttt tccggtaatg
    13621 aagcttttcg atgcggttaa gcttgtcagg agcaagtatg atgctgcttt ggaggaatgg
    13681 gaacgggcag ccgtcaggca ggaacgcagg cgcgccgccc ttatgtcgcc ttcaggcctg
    13741 atgtcatgca ttcctttact gcttttattt gcagttggcg ggatgcaggt cgtcagggga
    13801 gagctgtcga cgggcggcct gtatattttc atcaaccttt ccgggattgt gtccggtgtg
    13861 atgatgaaca tgccgggccg gatcgcttcg ttccggaggt ttgccgtgaa cctgagacgg
    13921 atcgggcctt atatgacagc aggctttgca gccggaaagg ctgacggggg atcaggggtt
    13981 tctgccgggg tataagcgat atagccggcc tggcgttgga agctgccagg atatacagaa
    14041 agatgcaatg aaaacggtgc aagaagaaag atagtacaca gtcaattaca gtaatgaagc
    14101 attctgatac agagatgaca ggcacagaaa tgaaaggtga tgctggatga gcataagagc
    14161 cgaaaatctg tggttcacat ataatgatgc gccggtcctg cgcggtatcg acctttttgc
    14221 agacaccggg gaacatatag gcatcacagg tatgagcgga tgcggcaaaa gcacgctgct
    14281 taagctgatg gcagggctat accgggcgca ggacggagcc gtggaggttc acggagcgcg
    14341 caggcctgag gacatccgca aaaatgttgc gatggtgatg caaaatgccg tgctgttccc
    14401 tgcgagtatc agggaaaaca ttacatgcgg gcatcaaatg agtgaagaaa atttacggca
    14461 ggtttgtgaa gccgcgcagc tcacggagtg gatcgcgtcg ctgccggacg gcattgatag
    14521 ctttgtcggg gaacggggcg gaaaggtatc cggagggcag gcgcagcgca tcgccatagc
    14581 ccgtgcgatc gctaaaaacg ctccggtgat tttgcttgac gaggcaacca gcgcgcttga
    14641 cagcgataca ggcagtgctg tcctggctgc gctggagagc ctgaccaggg ggaggactgt
    14701 cgtgagcgtg agccacaggt atgaaacgct ggcaggatgc agccgcattt ataaacttga
    14761 cggcggccgg ctttatgaat tgtgagat
//
LOCUS       DAOSCC010000031        14385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2861_length_14385_cov_4.637334, whole genome shotgun sequence.
ACCESSION   DAOSCC010000031 DAOSCC010000000
VERSION     DAOSCC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14385
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_14385_cov_4.637334"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLG72_03025"
     CDS             complement(<1..832)
                     /locus_tag="PLG72_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ79805.1"
                     /translation="MQKDENRSKYSENQTKYSMLKAYLKDEILMGNIRPGEKIPSENN
                     LAERFSLSRQTVRKAISVLVHEGFLYTRQGKGTFCKDRTAGRRESRNIGVVTTYISEY
                     IFPRVIQGIDKVLSENGYSIILKNTGNSIEKEAACLDDLLSKDIGGLIIEPTKSAVLS
                     DNLKYYRALDKHGIPYVFIHGSYKMLEDKPRVLLDDEAGEYLATEHLIKLGHRNIVGI
                     FKVDDIQGINRHKGYARALADGGIAYDPGKVVWFHTEDRDTKPGAAVRDIIAGGVRID
                     A"
     gene            1049..2545
                     /gene="araA"
                     /locus_tag="PLG72_03030"
     CDS             1049..2545
                     /gene="araA"
                     /locus_tag="PLG72_03030"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOJ79806.1"
                     /translation="MQDQKNFTFWFVTGSQHLYGPETLEMVDAHSRTIAEGLDKSPYV
                     PYKVVFKPVLTTPDAIRRLCLDANSDDSCAGIITWMHTFSPAKMWIAGLSELRKPMLH
                     LHTQFNRDIPWADIDMDFMNLNQSAHGDREFGFIGARMRIARKVVVGHWEDPDTCRRI
                     GLWMRSAVAYTLGASLKVARFGDNMREVAVTEGDKVEAQIKFGWSINGYGIGDLVDRI
                     KNVTDSDIDKLVQEYGELYEIPAEVRSSREAMDAVREQARIEIGLRSFLTEGGFGAFT
                     TTFEDLHGMKQLPGLAVQRLMADGYGFGAEGDWKTAAMVRMTKIMTKGLPGGTTFMED
                     YTYHLAPGNEMILGAHMLEVCPTVAGTKPRLEVHPLSIGGKAAPARLVFDGAAGRAVA
                     ASLVDMGNRFRLIINEVEAVKVEKDMPKLPVARLLWKPLPSLKQGAEAWILAGGAHHT
                     VFSFAATAEQLIDWAEMSGIEYVLINENTNILNLRNELRWNELAYDRR"
     gene            3139..3816
                     /locus_tag="PLG72_03035"
     CDS             3139..3816
                     /locus_tag="PLG72_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ79807.1"
                     /translation="MLDMYNPYGFNNAYLPPNCNKMQAYGPGKQYGVQANTPGSAYGV
                     YGIHQYINPYLNANQYQNMYQSPCPCSAQGQYHMPGTIYGSERPAIQDYGPAPLVINI
                     EDAASQNNTFRSALWTGKHLQVTLMSIMPGEDIGLEMHPDVDQFIRIEDGNGFVTMGD
                     SRDMLDFRRYVSDDHIIVIPAGKWHNLVNIGREPLKLYSIYAPPQHPHGTVHQTKAEA
                     EAAERSH"
     gene            complement(3853..4725)
                     /locus_tag="PLG72_03040"
     CDS             complement(3853..4725)
                     /locus_tag="PLG72_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ79808.1"
                     /translation="MWIVFAFGSAIFAGLTAILSKIGIRNTDSNVATAVRTIVVLVFS
                     WLMVFIAGSHTTITDISGYTLLFLVLSGLATGASWLCYFKALQIGDVNKVTPIDKSST
                     ILTMIMAFIFLGEDITLIKAAAIVLIGAGTYLMIQKKEAADTDSAPKKGGYLWLIYAV
                     GAAVFASLTSILGKIGIQGVESNLGTAIRTIVVLIMAWVIVFITKKQHTIKDIDAKSM
                     LFLVLSGFATGGSWLCYYRALQDGPASVVVPIDKMSIVVTIAFSSLILKEKLTPRSLI
                     GLVMISAGTLSLLI"
     gene            complement(4933..6351)
                     /locus_tag="PLG72_03045"
     CDS             complement(4933..6351)
                     /locus_tag="PLG72_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ79809.1"
                     /translation="MVSRKARSTAACLAVFMLAVIILAGCGAKSGGQASTDADGSAPG
                     ASAEMPDEADSVPAPAEPEEGQLPDEDEGSEDNGEGQPAEAGDASADQTPADTSAQED
                     ENRIIIPEINIEKRPIPDNEALAFVKDMKAGWNLGNTFDAVDVTGLSNKLDYEKAWVG
                     VKTTEAVAIAVKNAGFNTIRIPVSWHNHVSGDDYKIDEDWLNRVQEVVDYAVKHDMYI
                     IINIHHDMGKEYIYTSKEYLEQSEKYVKSIWSQVAERFKDYDHHLIFESLNEPRMVGH
                     SNEWWLDLKKQDCRDAVECLNELNQVFVDTVRSSGGNNKDRYLMVPGYAASYAGALAD
                     GFRIPDDTADNRIIVSVHAYTPYNFALEAGSVSTFDIGKQSSTSEIDYFMTELYSKFV
                     SKGIPVVIGEFGARNKNNNLQDRVNFTAYYIASASARGITCIWWDNHSFIGDGELFGL
                     LDRRYYRWRYPEIVEAIMKYAQ"
     gene            complement(6678..7475)
                     /locus_tag="PLG72_03050"
     CDS             complement(6678..7475)
                     /locus_tag="PLG72_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79810.1"
                     /translation="MTEQEFNKIVVDIHYYNHRICTPSWIIEERLNSFVDLTYVVAGK
                     AEYTIDGVCYTVRAGDLICVRKGCRESAVAVPDDLMDCYSVNGYIYDLTGREMVLPFK
                     TVSHIGVRQDIIALYQELNAAWLVRDPGYMAKARAIFMLILHRFYQLICHSGYQRNYD
                     KRIKKVLQHVIEHYFEPLTVTEMAKMVNLSPLYFGNFFKQETGQTFRQYLTLIRLNQA
                     ESMLNSGMYKVKEVAVECGFSDIYYFSKVFKEHFGIAPSEVLKSSTP"
     gene            7886..8743
                     /locus_tag="PLG72_03055"
     CDS             7886..8743
                     /locus_tag="PLG72_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ79811.1"
                     /translation="MLVFPIAFFVIFRYLPMTNIVMAFKDYNMFKGVWQSDWAGFKWF
                     EKAFASKDFWRSLRNTIMLNFLDLLMGFPAPIILAILLNEVVFYRFKRVTQTIVYLPH
                     FLSWIIISGMAFQLLAPVSGVINQFLAKLGAGPVEFLGDKKLWVCTYVVLGLWKEVGW
                     NAIIYLAAISGINTELYEAAEIDGAGRFRKIWHVTLPGIRSTIIVLLVMNLGRILGIE
                     FDRPYSLQNTSVMDVADVISTYVYRVGIRGLQFSLTAAVGLFQSVVCVIFLLTANAIA
                     KKFGERGIW"
     gene            8758..9678
                     /locus_tag="PLG72_03060"
     CDS             8758..9678
                     /locus_tag="PLG72_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ79812.1"
                     /translation="MDTARNTQFAEISMVSEKRRRTRRLKTGDCVIAAICVFLIIIYL
                     APFLNIIARSLSSPNALVNNRVSFWPVDFTPDSYVYVFSDAAFSRSLVFTAILTVICT
                     IVSMTMTVFAAYPLVYDLKGGRFINLMIIFTMYFSAGTIPNYLLMKQLHLLDNPLVLI
                     LPNCLSVFNMIILRSFFFNIPDSLRESAELDGAGPVRILMNIYLPLSGPVLATLALFY
                     AVGRWNGFSDALMYITDIKYQPIQLKLWQIINNRQSIEIASQEGFATPAASEGLKAAS
                     VMFATVPILVVYPWLQRYFIAGVTIGAVKG"
     gene            9738..11375
                     /locus_tag="PLG72_03065"
     CDS             9738..11375
                     /locus_tag="PLG72_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ79813.1"
                     /translation="MKKVLVLLLAFAMLLTAAACGSKTESGNAVPAPSSGSDTSAQTS
                     SDKPADTKPERTKIRVEVFDRGTDGGKTDPTNNYWTDWIKAKVLEDENIEVEFVAVSR
                     YEETDMLNNLMASGTAPDVCYTYSGDLIALYRDQGGLTNLVPYMDLLKDLDVLLGEDT
                     SLPGRRLIERNRVLETGEIHSVPARRMNVAGANTFIRKDWLDKLGLPIPNTRQEWTDA
                     LRAFKNNADKLGVDVIPFTMTPDIRWRAGNLIDSFIDPNISPKDRWINTVVDRNFLLP
                     GVKEGYRYLNMMYLEGLIDPEFYLYKDDVKNEELIKAGRVGSFIHNWDQAFRNSPSLL
                     DGLRENVPDGEYVACDPFENAAGKKYKMKYNAAGLFVFIPASSKVPEAAVRYINWLAK
                     PEIYRFLQFGEEGKNHKIVNGLPATIPATGPTIQNSVNNIDYTLNMNGIWLGDEETTA
                     KAIANGYTCDPKYIIDAMTCAMNDAEAGLVVPVTLSVAGPYTEDLTAKGEEGTCNAVR
                     APAGQFDAIWDAFIADWRASGADEIIAERAAKYVDPF"
     gene            complement(11489..12667)
                     /locus_tag="PLG72_03070"
     CDS             complement(11489..12667)
                     /locus_tag="PLG72_03070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ79814.1"
                     /translation="MDNFTFYSPTYFVFGKDQENETGKYVKRFGGSRVLIHYGGGSAV
                     RSGLIDRVKASLEREGLSYTELGGVQPNPRAGLVYKGIDLCRKEKVDFILAVGGGSVI
                     DSAKAIAAGVPYDGDFWDFYKGRPVTEALPIGTILTISAAGSEGSPNSVITNENGMYK
                     RGAAGEALRPVFSILNPALTQTLPPYQVACGITDMMAHLFERYFTRTSNVEVTDRMIE
                     GLLLAIINEGPKAVKDPADYDAQANLMWAGMMAHNNSCGVGRSQDWASHGMEHELSAL
                     YDVPHGAGLAVVFPAWMTYILERGGVNRLAQLAVRVWGCQMDFKNPENTARAGIEALK
                     RFLKSIGMPSNFAELGAKEEDIGKMAHKACYGNGGSGAIGGFVRLNEEDVANIYRLML
                     "
     gene            complement(12729..13544)
                     /locus_tag="PLG72_03075"
     CDS             complement(12729..13544)
                     /locus_tag="PLG72_03075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ79815.1"
                     /translation="MKYKLITLDIDGTLINSAGIMTPETVEAIRRCRDAGVMVTISTG
                     RPIQGVRDFIDELKLDAPIITYNGAMIVDAATGGILFEERLLRRDAEQVLRLGREMDT
                     TIIAWSNNELYVNKINDRTDDYKKLSGNEPVVFSDADRILDQGITKIIWIDDVDRIEA
                     FKMGIKGIVNDSVTYCTSQPHFLEFFSSSVSKAVALEFIGRRYGIRREEMIAIGDGFN
                     DLSMIEYAGLGVAMGNAAEGVKERADYVTASNDEDGVAKVIYRFVLENGHKSG"
     gene            complement(13541..>14385)
                     /locus_tag="PLG72_03080"
     CDS             complement(13541..>14385)
                     /locus_tag="PLG72_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ79816.1"
                     /translation="VRNYMHVESYFKRAAADEAADGEEDETRTGSIPEKWLTTDEVDR
                     ILAQTSFANQGKRSSWKKHKNTYYSGRNGDEPRKGAPLSEPEGDEPDNVPDKGNPDAG
                     KAPDVSAVQEEREKYLLVDGYNIIFAWPELKDIAKDNMDSARTRLLDLLSKYQGMRKC
                     HVITVFDAYRVQRNHEDVFDYDNVHVVFTKEAQTADEYIEKFTHDNHKKYDITVATSD
                     NLQQIIILGAGCSVLSAGDLKKEIESLEESISKAWLDRQVRHRNYLRDLLPDEMKKRM
                     KEDL"
BASE COUNT         3481 a         3861 c         3406 g         3637 t
ORIGIN      
        1 ccgcatcgat acggacgcct cctgcgatga tatccctgac agcggcgccc ggcttggtat
       61 ccctgtcttc tgtgtggaac catacgactt tcccgggatc gtacgcgatc cctccgtcgg
      121 ccagtgctct cgcataacct ttatgcctgt tgatgccctg gatatcgtcg actttgaaaa
      181 tgccgactat gtttctgtgt ccaagcttta taagatgctc tgtcgccaga tactcgccgg
      241 cctcgtcgtc aagcagcacc ctgggcttat cctcaagcat cttataggac ccgtggatga
      301 agacataagg tatgccgtgc ttgtcaagag ccctgtaata tttgaggttg tcagaaagca
      361 cggcgctttt ggtcggttct atgatcagcc cgccgatatc cttgcttagc agatcgtcca
      421 ggcaggcagc ctccttttct atgctgtttc cggtgttttt caggattatg ctgtatccgt
      481 tctcagacag caccttgtct ataccttgta tgaccctcgg gaaaatatac tcggagatat
      541 atgtggtcac tacccctata ttccttgatt ctctcctgcc cgcggtcctg tccttgcaga
      601 acgtcccctt tccctgcctg gtatacagga atccttcgtg cacaagtacg gaaatggcct
      661 tcctgactgt ctgccggctg agcgaaaatc tttccgccag gttgttttca gacggtatct
      721 tttctcccgg cctgatattt cccatcagta tctcatcttt gagataagct ttcagcatcg
      781 agtattttgt ctggttttca gagtattttg aacggttttc atctttttgc atacagcaga
      841 tttgcctttc ttgtacgtac atgttcgcat tacaaattat acaccatccc gttgcgatta
      901 tcaacggata catcgtgcaa agcccgcatg caggcccaat gttccgtgga tccggatgca
      961 aagccgccgt tgcttttaca caggcaaagt agtagaataa ttatatgaac ttgttcgtac
     1021 atgttatact ggtaaggagg tatagcttat gcaggatcag aaaaatttca ccttctggtt
     1081 cgtaacggga agccagcact tatatggacc tgaaacactg gaaatggtag atgcccattc
     1141 aaggacgatc gcagaaggtc tggacaaaag cccttatgta ccgtacaaag tcgtattcaa
     1201 acctgtactt acaacaccgg acgcaataag gagactgtgc ctcgacgcca attcggatga
     1261 cagctgcgcc ggcatcataa catggatgca tacattctcg cccgcaaaga tgtggatagc
     1321 aggcctttca gaactccgca aacctatgct ccacctgcac acccagttca accgcgatat
     1381 cccgtgggcc gacatagaca tggatttcat gaatctgaac cagtccgccc atggcgaccg
     1441 tgaattcgga ttcatcggcg cacgcatgcg cattgcccgc aaggtagtcg tcggccactg
     1501 ggaggacccg gacacatgca ggcgcatagg cctctggatg cgctccgctg tcgcatatac
     1561 gctcggagca agcctgaaag tcgcaaggtt cggcgacaat atgcgcgaag tggcggtaac
     1621 cgaaggcgac aaggtggaag cccagatcaa attcggctgg tcgatcaacg gctatgggat
     1681 aggagacctt gtcgaccgca tcaaaaatgt aaccgacagc gatatcgaca agcttgtaca
     1741 ggagtatggc gaactttatg aaataccggc cgaagtgcgt tcttctaggg aagcgatgga
     1801 tgcggtaagg gaacaggccc gcatcgagat cggcctgaga tctttcctta cggaaggagg
     1861 ctttggggca ttcaccacca cattcgaaga cctccatggc atgaaacagc ttcccggcct
     1921 tgcagtacag aggctaatgg ctgacggtta cggcttcgga gccgagggag actggaagac
     1981 ggcagcgatg gtccgcatga cgaagatcat gaccaagggg ctccctggcg gcacgacttt
     2041 catggaagat tacacgtacc acctggcgcc gggcaatgaa atgatactcg gagcccatat
     2101 gctcgaggtc tgtccgacag tagcaggtac aaaacccagg ctcgaagtcc atccgctgtc
     2161 catcggcggg aaagccgctc cggcccgtct cgtattcgac ggcgcagcgg gcagggcagt
     2221 cgccgcttcc ctcgtcgaca tgggcaaccg cttcaggctg atcataaatg aagtggaagc
     2281 agtaaaagtt gaaaaggata tgccgaaact cccggtcgcc aggctgctgt ggaaaccgct
     2341 gccgtcgctc aaacagggag ccgaagcatg gatactggca ggcggcgcgc accatacggt
     2401 cttctcgttc gcagcaacgg cggagcagct tatcgactgg gcggaaatgt ccggcataga
     2461 atatgtgctc ataaacgaga acaccaatat cctgaacctg aggaacgaac tcaggtggaa
     2521 cgagttggcg tacgacagga gataacatgg cagcgtgatc acgaaaacag ccatgtgatg
     2581 tttttgtgaa aaaccttatg acgcctttag acgtacgccg acacaaaaaa cagcataaag
     2641 ccaggcagca attgctgaag aagcatgccg tttcataaac cggcatgctt ctttctgttc
     2701 acagacattc ttttttattg gtctgcagta gtaatacagt tttcgggtaa ggaccagggt
     2761 gtacattttt ttaattgttg caatatcgtt tgatcatttt ttcatataaa aggaaccata
     2821 aacaggcaaa ttcctgtatt atgtaaaatc gtttaatgag aattatcaat atatacgccg
     2881 cttaaacaca caacaattaa aattttgtac accctgctct atatttcccg gggaaataaa
     2941 catctggcaa ggagtcctct aaaaatcggg cattgacaga taagataacg gcagatccga
     3001 tgaaacagat ctggaaaaaa ataaatccga atgaagaaga catatccggc aaatttgaag
     3061 catgatatat acctgcacgg ctcattgaca taaagcataa aataaagcgt gatccataat
     3121 caggaaaggt ctgatcttat gcttgacatg tacaacccat atggatttaa caacgcgtac
     3181 ctgccgccca actgcaataa aatgcaggca tacggtcccg ggaagcaata cggagtgcag
     3241 gcgaacactc cgggcagcgc atatggagta tatggcatcc atcagtacat caacccatat
     3301 ctgaacgcaa atcagtatca gaacatgtat cagagtccgt gcccctgctc cgcacaaggc
     3361 cagtaccata tgcctgggac catatacgga agtgagcgtc cggcaataca ggattacgga
     3421 ccggctccgc ttgtcatcaa catcgaagat gcagcttcgc aaaacaatac gttccgcagc
     3481 gcattatgga cagggaaaca tctgcaggtc actctaatga gcatcatgcc cggcgaggac
     3541 atcggactgg aaatgcatcc cgacgtggat cagttcatcc gcatcgagga cggcaacggg
     3601 ttcgtaacga tgggcgacag cagagacatg ctggatttcc gccgttatgt aagtgatgat
     3661 cacatcatcg tgatacctgc cggcaaatgg cacaaccttg tcaacatcgg gcgggagcct
     3721 ctgaagctgt attccatata cgcgcctccg cagcatccgc acggcacggt ccaccagaca
     3781 aaggcagaag ccgaagccgc tgagcgcagt cattgagaac aggcgccgtt gcccccgttt
     3841 tgcttcagaa tgtcagatca gcagcgacag cgtgcccgcc gatatcatta caaggccgat
     3901 caatgatctg ggcgtgagct tctctttcag aatcagggaa gagaacgcga tggtgacaac
     3961 tatgctcatt ttgtctatcg gcaccaccac gcttgcaggt ccgtcctgca gagccctgta
     4021 atagcacagc catgaaccgc ccgtggcaaa accggacagc acgaggaaca gcatgctctt
     4081 tgcatctata tcctttatgg tatgctgctt ctttgttata aaaacgatta cccacgccat
     4141 gataaggacc actatcgttc ttattgccgt gccaaggttc gactcgacgc cctgtatccc
     4201 gattttcccg agtatggatg tcaggctggc gaacaccgca gcgccgacgg catagatgag
     4261 ccagagatat ccgccctttt tcggtgcgga gtccgtatcc gccgcctctt tcttctggat
     4321 catcaggtat gtgccggcgc cgatcagcac gatagccgca gccttgatca gcgttatgtc
     4381 ttccccgagg aaaataaagg ccattatcat cgtcaggatc gtacttgact tgtcgatggg
     4441 ggtaaccttg ttgacgtcgc cgatctgcag ggctttgaaa tagcacagcc acgatgcgcc
     4501 tgtggcaagg ccggacaata caaggaacag gagcgtgtag ccgcttatat ccgtgatcgt
     4561 ggtgtgcgag ccggcaataa agaccatcag ccatgaaaaa acgaggacca cgatcgttct
     4621 gaccgccgtt gccacattcg aatcggtatt cctgatgccg atctttgata agattgccgt
     4681 cagccctgcg aatatcgctg aaccaaatgc aaaaactatc cacatttttt gtgcacttcc
     4741 ttaataaaat atttcctgct tatggtccat aaactgtcat aaaagccgtt ataatatgcg
     4801 gaacaaacga acgccggcgc ttgtggacag cacaaacacc ggcgcctgca tatcatcccg
     4861 cggaattgct ccaaagaccg atgggtcatg ccgtaaaaga ccgatggatc atcccacgga
     4921 ccgcatcaat gattactggg cgtacttcat tatcgcttcg acgatctccg gatacctcca
     4981 tctgtaatac cttctgtcca gcaacccgaa cagttcaccg tcgcctataa atgagtggtt
     5041 gtcccaccat atgcatgtta ttccccttgc ggaagcggaa gcgatataat acgctgtaaa
     5101 gttgaccctg tcctgcagat tgttgttttt gttcctagcc ccgaactcgc ctatcactac
     5161 cgggatgcct ttggaaacga atttgctgta cagctcggtc ataaaataat cgatctcgct
     5221 ggtacttgac tgtttgccta tatcaaacgt gctgacgctg ccagcctcaa gagcaaagtt
     5281 gtaaggcgta tatgcatgga ccgatactat gatcctgttg tccgcagtat cgtccgggat
     5341 cctgaaaccg tcagccagcg cgccggcata cgaagccgca taaccgggga ccatgagata
     5401 gcggtccttg ttgttgccgc cgctggaacg tacggtgtcg acgaagacct ggttaagctc
     5461 attcaggcac tcgacagcat ccctgcaatc ctgttttttg agatccagcc accactcatt
     5521 gctatggccg accatacgag gctcgttcag cgattcgaaa atcaagtggt ggtcataatc
     5581 tttgaatcgt tctgcaacct gtgaccagat acttttcaca tatttttcgg actgctccag
     5641 gtattccttt gacgtatata tgtactcctt gcccatgtca tggtggatat tgatgatgat
     5701 atacatatca tgcttaacgg cgtaatcgac gacttcctgt accctgttca gccagtcctc
     5761 gtcgatcttg tagtcatcgc ctgatacatg gttatgccat gacaccggga tgcgtatggt
     5821 gttgaaaccg gcattcttca ctgctatggc cactgcctcc gtagtcttga cgccgaccca
     5881 agccttttca tagtccagct tattggaaag tcctgttaca tccacagcat cgaatgtgtt
     5941 cccgaggttc caccctgcct tcatgtcctt tacaaaagcg agggcttcgt tgtcgggtat
     6001 gggccgcttt tcgatattga tctcagggat gatgatcctg ttttcatctt cctgtgcaga
     6061 tgtatctgcc ggcgtttgat ccgctgaagc atctccggcc tccgccggct gtccttcccc
     6121 gttgtcttcc gaaccctcgt cttcgtccgg caactgccct tcctccggtt ctgccggggc
     6181 aggtaccgaa tccgcttcgt ccggcatttc cgcagacgcg cccggcgcgg atccgtccgc
     6241 gtccgtcgat gcctgtccgc cggattttgc gccacaacct gcaagtatta tgaccgccag
     6301 cataaaaaca gcaaggcagg cggcggtcga tctcgctttt cgactgacca tattcttttt
     6361 cctccttttc tgtcttccat acatctactc aatacatctg caatgacaaa tatgccgtac
     6421 atacaaaatt ttaccgcata ccgcagccag ttacaagtaa atggccgtaa aaccccgccg
     6481 cgctgagata tttccgggac aggatatctg agaagtgtcg cgtttgatca ggaatgggga
     6541 cacttctcgg aaagaggaat ggggacattt ctctgcaagt gggtataaac aatggggaca
     6601 ttcctcatcc atagaggaat gcttcttgca gaggagtgtc cccacacctc ggaaagagga
     6661 gtgtcccctt tcctctttca gggtgtcgag gatttgagaa cttctgacgg agcaatgccg
     6721 aaatgctcct tgaatacctt gctgaagtag tatatgtcgg aaaacccgca ttcgaccgcg
     6781 acttccttca ctttgtacat tccgctgtta agcatgcttt cagcctggtt taaccttatc
     6841 agcgtcaggt attgcctgaa cgtctgtccg gtctcctgtt tgaagaaatt tccgaagtaa
     6901 agcgggctga gattgaccat ctttgccatt tctgtcacgg taagcggttc aaagtaatgc
     6961 tctatcacat gctggagcac cttttttata cgtttgtcat agtttctctg gtaaccgctg
     7021 tggcagataa gctggtagaa ccgatgcagg atcagcatga aaatcgccct cgctttcgcc
     7081 atgtagccag ggtcgcgtac gagccacgcc gcattcagtt cctggtaaag ggcgattata
     7141 tcctgccgta caccgatatg cgacactgtc ttgaaaggaa gtaccatttc cctgccggta
     7201 aggtcataaa tatacccgtt gacactgtag caatccatca ggtcgtcagg gaccgcgacc
     7261 gccgactcgc ggcagccctt ccggacgcaa atgagatcac ctgcgcgcac cgtgtagcaa
     7321 acgccgtcta tagtgtattc agccttccct gcgacgacat atgtcaggtc gacaaaactg
     7381 ttgagacgct cctctatgat ccaggaaggc gtacaaatcc tgtggttgta atagtgtata
     7441 tcgactacta ttttattgaa ttcctgttcg gtcataccga tcaccacaaa ctgattatat
     7501 catcgtacaa gtaaaagtac aaatgtaccc atgattatta caaattttgt ttgcttctca
     7561 attgtttgcc tccgcagttc tgctacacta tgtatgaatt gcattgaata tgcttgattt
     7621 cacgttagat ttttatataa attcaacaaa cttgtttaca tatttaacta taaacggaat
     7681 tactttgatt cttaccgggc aacgataatt tccaacttgc gaagatatct ttgacgtcag
     7741 tctgattgca tcatattaag acatacacat tactaatcct aaaaggaggg agtatgcctt
     7801 gagcagtaag tccgcagtac agcagtctcc gcggtctgca tcaaagtttg gcagttatct
     7861 tcgccgcacc tggccgctct atgtgatgtt ggtttttcca attgcatttt tcgtcatttt
     7921 caggtatctt cccatgacga atatcgttat ggctttcaag gactacaaca tgttcaaagg
     7981 cgtatggcag tccgactggg ctggcttcaa atggttcgag aaagcattcg catcaaagga
     8041 tttctggaga tctctgagaa atacgatcat gctgaacttc ctcgatttgc tcatgggctt
     8101 ccccgcaccg atcatacttg cgatactgct caacgaggta gttttctacc ggttcaaacg
     8161 cgttacgcaa accatcgtat atcttcccca cttcctatca tggataatca tcagcggtat
     8221 ggcattccag ctgcttgcgc cggtttcggg cgtaatcaat cagttccttg caaagttggg
     8281 agcaggccct gtcgaattcc ttggcgataa gaaactctgg gtatgcactt atgtggtatt
     8341 gggactctgg aaagaagtcg gctggaatgc gatcatttat ctggccgcga tctccggcat
     8401 caacacggaa ctctatgaag ccgccgagat agacggcgcc ggaagattcc gcaaaatatg
     8461 gcatgtcact ctgcccggga tccgctcgac cataatagtt cttctcgtta tgaaccttgg
     8521 ccgtatcctc ggcattgaat tcgaccgccc atattcgctg cagaacacat ccgtcatgga
     8581 tgtagccgac gtcataagca cctatgtata ccgcgtaggt atcaggggac tgcagttttc
     8641 gctcactgcc gcagtcggtc tgttccagtc ggtcgtctgc gtcatattcc tgctgacggc
     8701 aaatgcaatc gcgaaaaaat tcggcgaacg cgggatctgg taaaggaggg aatatagatg
     8761 gacactgccc ggaatacaca atttgcagag atttcgatgg taagtgaaaa acgccgccgc
     8821 accaggagat taaaaacggg cgattgtgta attgctgcga tctgtgtttt tcttatcatc
     8881 atttatcttg cgccgtttct gaatatcata gcacgttcac tcagttcgcc gaatgcactg
     8941 gtcaataaca gggtctcgtt ctggccggtg gacttcacgc cggattccta cgtatatgtt
     9001 ttttccgatg ccgcattttc acgttcactg gtatttacag cgatactgac cgtgatctgc
     9061 acgatcgtct caatgaccat gacagtattc gccgcctacc ctctcgtata tgatctcaaa
     9121 ggcggcagat tcataaatct catgatcatt tttactatgt atttcagtgc aggcaccatt
     9181 cccaactatc tgctgatgaa gcagctccat ctgctggata accctcttgt gctcatactt
     9241 ccgaactgtc tgagcgtatt caacatgatc atactccgga gcttcttctt caacataccg
     9301 gacagcctgc gcgaatcagc cgaactggac ggagcgggtc cggtcaggat acttatgaat
     9361 atatacctgc cgctgtccgg tcctgtgctg gcaacacttg cactgttcta tgccgtcgga
     9421 cgctggaacg gtttttccga tgcgctgatg tacatcaccg atataaaata tcagcctata
     9481 cagctaaagc tctggcagat catcaacaac aggcagtcta tcgagatcgc ttcccaggaa
     9541 gggttcgcca cccccgcggc aagcgaaggg ctgaaggcag cctcagtcat gtttgcgaca
     9601 gttccgatcc tggtcgtgta cccgtggctg cagcgctatt tcatagccgg cgttacgatc
     9661 ggggctgtga aaggctgatc tggcaaccaa aggcgaaaaa ccgccttgga tataaaaatt
     9721 aagggaggta cttcatcatg aaaaaagtat tggttctgct gctggcattt gctatgctgc
     9781 tgaccgccgc cgcctgcggc agcaagaccg aatcgggaaa tgctgtacct gcgccgtcat
     9841 caggcagtga cacatcggcg cagacaagca gtgacaagcc ggcagatacg aaacctgaaa
     9901 ggacaaaaat cagggtcgag gtatttgatc gcggcactga cggtggaaag accgacccca
     9961 ccaacaacta ctggacagac tggataaaag caaaggtact ggaagacgaa aatatcgaag
    10021 ttgagttcgt cgcagtatcc cgttatgaag agactgatat gctcaacaac cttatggcca
    10081 gcggaacagc gccggatgtc tgctatactt acagcggaga cctgattgcg ctgtaccgtg
    10141 accagggcgg tctgaccaac ctcgtaccgt acatggatct gctgaaagat cttgatgtgc
    10201 tgctgggaga agacacatca cttcccggac gccgcctgat cgaacgcaac agggtccttg
    10261 aaacaggaga gatccactct gtacccgcac gccgtatgaa cgttgcaggt gcaaacacat
    10321 tcatccgcaa agactggctg gacaaactgg gacttcccat accgaacacc cgccaggaat
    10381 ggaccgacgc actcagggca ttcaagaaca atgccgacaa gctcggagtg gatgtaatac
    10441 cgtttaccat gacgcctgat attcgctggc gcgcaggcaa cctgatagac tcgttcatag
    10501 atcccaacat ttcaccgaag gaccgttgga tcaatactgt tgtagacagg aatttcctgc
    10561 tgcctggcgt aaaggaaggt tacaggtacc tcaacatgat gtacctggaa ggcctgatcg
    10621 accccgagtt ctacctgtat aaggacgacg tgaagaatga agaactgatc aaggcaggac
    10681 gcgttggttc gttcatccac aactgggacc aggcattccg caattctccg agcctcctgg
    10741 acggcctcag ggagaacgtg cccgacggcg aatatgttgc ctgcgacccg ttcgaaaatg
    10801 ccgccggcaa gaaatataag atgaagtata acgctgcagg gctcttcgta ttcattcctg
    10861 cgtcgagcaa agtgcccgaa gcagccgtac gttacatcaa ctggcttgca aaaccggaaa
    10921 tatacaggtt cctgcagttt ggcgaagaag gcaagaacca caagatagtt aacggcttgc
    10981 cggcaaccat ccctgcaacc ggaccgacga tccagaactc agtgaacaac atcgattata
    11041 ccctcaatat gaacggtatc tggctcggcg acgaagaaac cactgcaaaa gctatagcca
    11101 acggatatac atgtgatccc aagtatatca tcgacgccat gacatgcgcg atgaatgacg
    11161 ctgaagcagg tctggtcgtt cccgtcacgc tgtcggtagc aggtccgtat actgaagacc
    11221 ttacggccaa aggtgaagag ggtacctgca acgcagtcag ggctcccgca ggacagtttg
    11281 acgcgatctg ggatgcattt atagctgact ggcgtgcttc cggcgctgac gagatcatcg
    11341 cagagcgcgc agctaaatac gtagatccct tctgatgaat gattggcaga tatcagatag
    11401 ttcaatcagg gcatctggaa gaggcccgga ccgcatgatc gtccgggcct cttctttata
    11461 tctgcatcgg ttttgccgcc taagcgtttt acagcatcag gcggtaaatg tttgccacgt
    11521 cttcctcgtt cagcctgaca aaaccgccta tggccccgct gcctccattt ccgtagcacg
    11581 ctttgtgcgc catcttcccg atatcttctt cctttgcgcc aagctctgcg aaattcgacg
    11641 gcatcccgat ggatttaagg aacctcttca atgcttctat acctgccctg gcggtatttt
    11701 caggattttt gaaatccatc tggcatcccc acacgcgtac agccaactgt gccagcctgt
    11761 ttacaccgcc gcgctccagg atgtacgtca tccacgccgg aaataccact gcaaggcctg
    11821 ctccgtgcgg aacatcatag agcgcggaca attcatgctc catgccatgg cttgcccagt
    11881 cctgcgacct tccgactccg catgaattgt tgtgcgccat catgcctgcc cacatcaaat
    11941 tcgcctgggc gtcataatcg gcaggatcct tcacagcttt cggtccttca ttgattattg
    12001 ccagcagcag tccttctatc atcctgtcag tgacctcgac attgcttgtc ctggtgaagt
    12061 agcgctcaaa caggtgcgcc atcatatcag tgatcccgca tgcgacctgg tacggcggca
    12121 aagtctgagt cagcgccgga ttcagaatgg aaaacacggg ccttaaggct tctccggccg
    12181 ctccgcgttt gtacattcca ttctcgttgg ttataactga attcggcgag ccttcgctgc
    12241 ccgcggcaga aatagtcagt atcgtaccta tcggcagcgc ctctgtaacg ggcctgccct
    12301 tgtaaaagtc ccagaaatcg ccgtcataag gaacacctgc cgctattgcc ttggcagagt
    12361 cgataacgct gccgccgccg acagccagaa tgaaatcaac tttctctttc ctgcaaaggt
    12421 cgataccctt gtagacaagc ccggccctcg ggttgggctg tacgccgcca agctccgtgt
    12481 aactcagtcc ttcccgttcg agggaagcct tcaccctgtc gataaggccc gaccgtaccg
    12541 ccgaaccccc tccgtaatgg atgagcaccc tgcttccgcc gaatcgtttc acatatttgc
    12601 cggtctcatt ctcctggtct ttaccaaaca cgaaatacgt agggctgtaa aatgtaaagt
    12661 tgtccatatc tatcctcctt gtataaggta tggtgacact ccccggcaag atacgctgag
    12721 gaatgtcatc atccgctttt atggccgttt tcaagcacga acctgtaaat gactttggcc
    12781 acgccgtctt catcattgga agccgtcacg taatcagccc tttccttcac accttcggcg
    12841 gcgttcccca tggcgacgcc aagtcccgca tattctatca tcgacaggtc attgaacccg
    12901 tctccgatag cgatcatctc ctcccggcgg ataccatacc gcctgccaat gaactccagg
    12961 gcaacggctt ttgatacgct gctgctgaag aattcgagaa aatgcggctg tgacgtgcaa
    13021 tatgtcacac tgtcattcac tatacctttt atacccattt tgaaggcttc tattctgtca
    13081 acatcgtcga tccatattat ttttgttata ccctggtcca gtatcctgtc tgcatcggaa
    13141 aaaacgacgg gttcattgcc cgaaagtttt ttatagtcgt cggtgcggtc attgatttta
    13201 ttgacataga gctcattatt ggaccaggct atgatcgtgg tatccatttc ccggccaagc
    13261 ctgagcacct gttcggcatc ccgcctcaac agcctctctt cgaacagtat cccgccggtc
    13321 gccgcatcga cgatcatcgc gccattgtat gtgattatcg gcgcatcgag cttaagctcg
    13381 tcaataaaat cccgtactcc ctggatgggt ctccccgtgc ttattgtgac catgaccccg
    13441 gcatccctgc acctcctgat ggcctcgacc gtctccggcg tcatgatccc ggcgctgttt
    13501 atcagcgtcc cgtcgatatc caaagtgata agtttgtatt tcatagatcc tccttcatcc
    13561 tctttttcat ttcatcgggc aacagatccc ttaggtagtt cctgtgccgt acttgcctgt
    13621 ccagccatgc tttgctgatg ctttcttcga ggctttcgat ctctttcttc agatcgccgg
    13681 cggacaatac ggagcagccg gcgcccagga ttattatctg ctgcaggttg tccgacgtcg
    13741 cgacagtgat gtcgtacttc ttatgattgt catgggtgaa tttctctata tattcgtcgg
    13801 ctgtctgcgc ttcctttgta aacacaacat gtacattatc ataatcgaat acgtcttcat
    13861 ggttcctttg gacgcggtat gcgtcgaata ccgttatgac atggcatttc cgcatgccct
    13921 ggtatttgct caggagatcc agcagtctgg tcctggcgct gtccatgttg tctttagcga
    13981 tatccttaag ttccggccat gcaaatatta tattataccc gtcaacaagc aggtactttt
    14041 cccgctcttc ctgtaccgct gatacatcgg gcgctttccc ggcatccggg tttcccttgt
    14101 ccggcacatt atcaggctca tcgccttccg gctcgcttaa aggcgcacct ttgcgcggtt
    14161 catctccgtt gcgtcccgaa taataagtat tcttatgttt cttccatgaa gacctcttac
    14221 cctggttggc aaaagatgtt tgtgccagta tcctgtccac ttcatccgtc gtcagccact
    14281 tctccgggat ggatccagtc cgtgtctcat cttcttcccc gtctgcagcc tcatctgcag
    14341 cggctctttt gaaataactc tccacgtgca tgtagttcct gactt
//
LOCUS       DAOSCC010000032        14315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2878_length_14315_cov_4.845231, whole genome shotgun sequence.
ACCESSION   DAOSCC010000032 DAOSCC010000000
VERSION     DAOSCC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_14315_cov_4.845231"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1905)
                     /locus_tag="PLG72_03085"
     CDS             complement(121..1905)
                     /locus_tag="PLG72_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79817.1"
                     /translation="MARNKFDIDEELEAKFDSKQLKRLLGYLKPYRGKVAFTVMLMIV
                     SSLLALLGPYLVKVSIDEMIPSGNIAGLFVLALIYFVILVFNTICMKYRIRTMSYIGQ
                     SVIYNIRDDIFRHIQKLPFDYYDSRPHGKILVRVVNYVNSLSDLLSNGIINVITDAFT
                     LVAIIVFLLVLNVKLALVCFTGLPVLAAITLLIKNAQRKAWQKVSRKQSNMNAYIHES
                     ICGMKVTQSFAREDENMNIFLGQNSEYRKSWIEAIKIQFLMWPMIDIISVTSVSAIFF
                     AGIHWLGTGGITTGIIIAFTSYVWRFWEPISNLGNFYNSIINAVAYLERIFETIDEKP
                     SVADLPGAVEMPQIKGAVEFKDVVFCYEKDEVILDHVSFSVRPGETVALVGPTGAGKS
                     TIVSLVSRFYDVREGSVMIDGMDVKSVTLESLRKQMGVMLQDTFIFSGTIMDNIKYSR
                     LDATDEEAIAAAKAVCAHDFIMTLPDGYKTEVNERGSRLSVGQRQLISFARALLADPR
                     ILILDEATSSIDTKTEMALQAGLQRLLKGRTSFIIAHRLSTIKNADRIMYIDNGMIIE
                     QGTHGELMEKKGAYCKLYTAQYQLLEAV"
     gene            complement(1906..3642)
                     /locus_tag="PLG72_03090"
     CDS             complement(1906..3642)
                     /locus_tag="PLG72_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79818.1"
                     /translation="MREFKWVWGLMRKYRFLFFVALGLVFAVNLLNLINPFVQGIIVD
                     DVIGKGQTDRLLTLVLIMVGNTVLRAIFRYTYLYMFENISQNVVYNARRDVFRKIQEL
                     DFDFFDRTKTGDIMARITGDLDAVRHFTAYVIYSVFMHAVTLILVLIVLFTINTTFTL
                     ILLAITPITGILAVRLSSTVKPAFSAIREQFSRLNSVVQENISGNRVVKAFAQEDYEI
                     YKFSRENAGFRDCNLDASKIWEKYLPVMDAVAGFLSVIIILAGGILVINGNITLGELV
                     TFNSFLWALNNPMRMIGWLINDTQRFATSAEKVMTLLNREPKIRSKEGAVSKKLEGRV
                     EFRDVSFRYGKEPVLEDISFCANPGQTVAIVGPTGSGKSTLVSLISRFYDCTRGTVLI
                     DGRDVKDYDVRTLRDNIAVAMQDIFLFSETIEGNIAYGASDVPVEKVQWAAELACAHE
                     FITEFPEGYDTIIGERGVGLSGGQKQRIALARAIVKEAPIIILDDTTSSVDIETEHQI
                     QQNLKEFCKNRTTFIIAHRISSVKHADLILVLEGGRIVERGTHEELLAMRGRYYNIFI
                     NQYGDFDKVVNE"
     gene            3813..4697
                     /locus_tag="PLG72_03095"
     CDS             3813..4697
                     /locus_tag="PLG72_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79819.1"
                     /translation="MCDLGIKPENFNPQVYFTFKGRYEENHVTKVHSHDFVTMIYVMS
                     GNCTYTINGASYRVRKGDMLVFNANVTHGKNVGAGEEIMELHVGLGNICIDGLPRNHL
                     VASDAPPVYHLQDYEQQFINCCSDICTEQENNLPGCELMIKVHVMRMMVLFMKATKTG
                     IHRSANTLSGIDSSDKAVIVNTLMKYMNENYMRQISLETISKKIYLSPAYISKVFKDE
                     TGETPINYLIKIRLAKAREMLMAGGRSVKSIARAVGYDDAYYFSKLYKKYHGVAPSKV
                     LKERGHSGLYSTQCNGSK"
     gene            complement(4667..5608)
                     /locus_tag="PLG72_03100"
     CDS             complement(4667..5608)
                     /locus_tag="PLG72_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ79820.1"
                     /translation="MEIQAEAQDQDPGKLHGTVDYVLQRTGNSISALNTDNTGNFPND
                     EDTGQHKGSDNEQDIADNKYGISDEQHGSNPAVITPAAPRLVAHAGGDIYGIRMTNSL
                     QALNKSYEEGFRYIEMDISLTSDGIPVFIHDWGNANWFAGIKYSTEEYGYEDFKKRTG
                     ILGLEFMDLDKLELWLEMHDDAYIITDIKENNVEVLGYIRNNYPEASKKIIPQIYSLD
                     EYEPVKNLGYEHIILTLYRIETIGDDVFEFCSEQPLFGLTMSQSRAKTDSLKKFSDLG
                     IPVFVHTVNDYNEYVKVRDNGAYGVYTDYFEPLHWVE"
     gene            6097..6360
                     /locus_tag="PLG72_03105"
     CDS             6097..6360
                     /locus_tag="PLG72_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79821.1"
                     /translation="MIVLVLLVFKMSKKELPFCRSFQHTVDGNTGGVVFLSFFLIAFM
                     AGIHFALSFAPPFGVIANIAVSVLLSVLLWRSCFRISWKDVEY"
     gene            complement(6400..7530)
                     /locus_tag="PLG72_03110"
     CDS             complement(6400..7530)
                     /locus_tag="PLG72_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ79822.1"
                     /translation="MKENSNLKIYSLSDIRNMKVHGRMTGRLSPLTLFWTGSGVEFNV
                     KGSELWIEVETDYDVYEPWISILINSYPVARQMVTAGRHWLCVFRGMNAGAAKNVRIM
                     KDVQPMSFDKGCLVHIHSVRFDGEFLPVEDRPYKIEFIGDSITSGEGTVGASCEQDWI
                     PMWFSAADNYTYLTAKALNADYRVVSQSGWGVLTGWDNNPNCSIPRYYEQICGILPKE
                     NCAVADAFGQNDFDEWQPDVIVVNLGTNDANAFSTPEWVDEASGARHKQRLNEDGTFN
                     EEDLAAFENAAAGFLGKLRKYNKNAHIVWAYGMFGAPLMPSIYRAADTYIRSTGDRKV
                     SIFQLPNTTNETMGSRSHPGKHAHEKAAKELTNYIIGLLPQK"
     gene            complement(7649..8128)
                     /gene="rlmH"
                     /locus_tag="PLG72_03115"
     CDS             complement(7649..8128)
                     /gene="rlmH"
                     /locus_tag="PLG72_03115"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOJ79823.1"
                     /translation="MKVTIIAVGKIKEHYLKEGIEEYSKRLSRFCDLRIIEAADEPAP
                     DDLSAAREEQIKSREASRVLGKVKDGDYLIVLDVKGAKKSSEEFAKVLKNCMISGNSS
                     ITFVIGGSLGLAPELIRKANVRLSLSDMTFPHQLTRLILLEQIYRAFKIINGETYHK"
     gene            complement(8386..9174)
                     /locus_tag="PLG72_03120"
     CDS             complement(8386..9174)
                     /locus_tag="PLG72_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ79824.1"
                     /translation="MKICSLFSGSSGNAVFIAAERTKLLVEAGLSGKKIAGAMCSIGE
                     SLSEISAILVSHEHIDHIRGAGVLSRKYDLPIYASRGTWEAMDQMIGPVRECNKKIFS
                     SYVPFEIGDITITPFPIPHDANEPVGYSFSSAGRKATIATDIGHISMDLLRNFEDSDL
                     LLLESNHDLEMLKMGPYPWPLKQRIAGKHGHLSNDAAGEVVAYMAERGTKKFLLGHLS
                     KENNFPELAYQTVRSVLCEKCVNAGADITLDVALRDRASRVIEI"
     gene            complement(9244..10506)
                     /locus_tag="PLG72_03125"
     CDS             complement(9244..10506)
                     /locus_tag="PLG72_03125"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ79825.1"
                     /translation="MEKIIIHGRKPLKGDIYISGAKNAAVAVLPAALLIEGSCRIENL
                     PDVKDTRILEETLTQLGAKVTFEDRNTVLIDSTDVNSYIASYDMIKSMRASYYLLGAL
                     LGRFGKAEVALPGGCDFGHRPIDQHIKGFEALGAKVVIENGMIKLSAEKLTGNQIYLD
                     VISVGATINLMLAAVMAEGTTVIENAAKEPHIVDTANFLNAAGADIKGAGTDVIKIRG
                     VKSLKGGNVHSIIPDMIEAGTFMIASAATDGDVTVRNVIPKHLESLSAKLQEMNINVE
                     EGDDWIRVTGGNGTIRKVNIKTLPYPGFATDLHPPVTVLLCLADGNSTITESIWPQRF
                     QYVDELRRMGAKIRANGGMAIIEGPVTLTGAQVNAIDLRAGAAVVLAGLAAEGRTEVS
                     DVKYIDRGYENFVVKLQSLGADIVRIEE"
     gene            complement(10873..11691)
                     /gene="yycI"
                     /locus_tag="PLG72_03130"
     CDS             complement(10873..11691)
                     /gene="yycI"
                     /locus_tag="PLG72_03130"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HOJ79826.1"
                     /translation="MDVSKAKNVVIVLLLVFNVFLLANMLIFRSSQGVSKETLENTRL
                     ILEQRGIRLECEIPSKPGGNSRLVYIKSELDGGNIAERLLGTKYEASGDGSHFFSSNG
                     KIEFTGIDSFIYTAGTEARAQDAVSVKEAEDAAMKFMKEKGLLAGKYVLDRSEENGDG
                     GWTLDYIETYGGSLLFDNCFSITLRGREVSRLEYRRHQFKGFSNENIEQFEAYQALLA
                     YFKEKSDMVITSIDSGYKLEEPSMGDVETVELLPVWRVKIKGMHEPVYISPHDT"
     gene            complement(11695..13020)
                     /locus_tag="PLG72_03135"
     CDS             complement(11695..13020)
                     /locus_tag="PLG72_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79827.1"
                     /translation="MGILWNYQNQGTPFSFLSALFSKDIQVSNEAIRENLFVPDKLVV
                     SNGDNDYWIISNDHDHYMKFFNEVIAGLSRIATGNAELNVSDEKWEEIVTKRGILVDF
                     GFYLEPDLLGWFLGMGSPVQDMPRFSKLMVRKDIVREDTGTFYICGDDGTVYSTDQVR
                     YEQAANLTNVISKLEEGSKNKYRKYKTLSGSKLNKSGDEPDVLYVVSSPRYWPYRVLS
                     ADLPAAANDEDMLANIILGDEEGRYNKYTYNGDMIQYTYGNNIYRYYSDGYLTYRYLG
                     SADTSGTKASEALMNAYKFMAGINELVGPETDIILSSVEKKAGGIYEFGFDYRVYGLK
                     VRTEYETKDGNGGKLGYAIRILADSRRVLECDWLIRSFRIGRTHNYNDRLMELLSNEN
                     ILWRNINISRIDTGYYILSSKDKLLEPALIITTGEGATAYLDMIAEEGD"
     gene            complement(13116..13931)
                     /locus_tag="PLG72_03140"
     CDS             complement(13116..13931)
                     /locus_tag="PLG72_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79828.1"
                     /translation="MLKKLSVVFIMLLIFIVAVSAAYAKPEEGNTGTSSKTTVNAPSG
                     TSATSNAAAALPANSIVAPSNIPASLPSNNSTQKDGGSQTGSNVAFKTTGTGETKTNA
                     ASAEKETSGGTEKDKEQKEDPDAAFWRAIRKVLEAGKPVSNGQSVVTIKKPENIEGDV
                     TTYGKTFEISFESEYTDVEILIAMLNSETGVYETIELPDGDRVLNIGSGALSTELELD
                     PGEYDLLLIAYRTSEKDPEHIQYTPIAVTVFKDSWWAQLKKTGKQIIDWFGSK"
     gene            complement(14076..>14315)
                     /locus_tag="PLG72_03145"
     CDS             complement(14076..>14315)
                     /locus_tag="PLG72_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ79829.1"
                     /translation="VYFKVVDTGIGIPEEDIDRIFERFYRVDKARSREMGGTGLGLSI
                     AKEIVEAHNGSINISSKVGAGTEVTVRLPMRQTSQ"
BASE COUNT         3309 a         3802 c         3151 g         4053 t
ORIGIN      
        1 ctggtagcgc ccgctgtagc gcgaagccac gggcaccgga taccggcccg gctctggcca
       61 aacacagcgc cgggacacgg ccaggcagtg acggaagctg aacacggtac cgggtttatt
      121 ctaaacagcc tcgagcagct ggtactgcgc cgtataaagc ttgcagtacg cgcctttctt
      181 ctccatcagc tctccatgcg tgccctgctc gattatcatc ccgttgtcta tatacattat
      241 cctgtctgca ttcttgatcg tggaaagcct gtgggctatt atgaacgacg tccgcccttt
      301 cagaagcctc tgaagccctg cctgcagagc catctcagtt ttggtgtcga tgctcgaagt
      361 ggcctcgtcg aggatcagta tcctcgggtc ggcaagcagc gccctcgcaa atgagatcag
      421 ctgcctctgc ccaacggaca gccttgaccc tctttcattg acttctgtct tataaccatc
      481 cggcagcgtc attatgaaat cgtgtgcaca gacggctttg gcggcggcaa tggcttcctc
      541 gtccgtcgcg tcgagcctgc tgtatttaat gttgtccatt attgtgcccg agaatatgaa
      601 cgtatcctgc agcattacgc ccatctgctt cctcagcgac tcaagcgtta cgctttttac
      661 atccatgccg tcgatcatta cgcttccttc ccgcacatcg tagaaccggc ttaccaggct
      721 gacgatggtc gatttgcccg ctcccgtggg ccctaccagc gcaacagtct caccgggcct
      781 gacgctgaag ctcacatggt ccaggatgac ctcatccttt tcgtagcaga acacaacatc
      841 cttaaattcc accgctccct ttatctgcgg catctcaaca gcacccggca ggtctgccac
      901 cgagggtttc tcgtcgatgg tctcgaatat cctttcaagg tatgcgactg cgtttataat
      961 ggaattgtag aagttgccca ggttgctgat aggttcccag aaccgccaca cgtagcttgt
     1021 gaaagcgatt atgatacccg tggtgatccc gccggtcccg agccagtgaa tacctgcgaa
     1081 aaatattgcg ctgacgcttg tcacagatat aatgtctatc atcggccaca tcaggaactg
     1141 tatcttgatg gcttcgatcc acgacttcct gtattcgctg ttttgaccga ggaagatgtt
     1201 catgttttca tcctccctgg caaaagactg ggtcactttc atgccgcaga tgctttcatg
     1261 tatgtatgcg ttcatgttcg actgtttcct gctgactttc tgccatgcct tacgctgggc
     1321 atttttgatc agtagcgtga tggctgccag taccggaagc ccggtaaaac agacaagcgc
     1381 caacttcaca ttaaggacaa gcaggaagac tattatcgca acaagggtaa aagcgtccgt
     1441 aataacgttg ataattccgt ttgaaagcag gtcgctgaga gaattgacat agttaacgac
     1501 ccttaccagt atctttccgt gcggcctgct gtcataatag tcgaagggca gtttctggat
     1561 atgcctgaag atatcgtccc ttatgttgta aataacgctc tgccctatat acgacatcgt
     1621 ccttatcctg tacttcatgc atatcgtgtt gaatacaagt attacaaaat atatcagggc
     1681 aagtacgaac agtccggcta tattcccgga aggtatcatc tcgtcgatgg agaccttgac
     1741 caggtacggc cccagcagcg cgagcaaact tgaaacgatc atcagcatca cggtaaaagc
     1801 caccttgcct ctgtaaggct tcaggtatcc cagcagtctt ttcagttgct tcgaatcaaa
     1861 tttcgcttca agttcttcat ctatgtcaaa cttgttcctt gccatctatt cgttcaccac
     1921 cttgtcaaaa tcgccgtact ggtttatgaa tatgttgtaa tagcggcccc tcatcgccag
     1981 gagttcttcg tgcgtgcctc tctcgactat cctgccgcct tcgagcacca ggatgagatc
     2041 ggcatgtttc acagacgaga tcctgtgggc gattatgaat gtagtcctgt ttttgcagaa
     2101 ttccttgagg ttctgctgta tctgatgctc cgtttcgatg tccacgcttg atgtggtatc
     2161 gtcaagtatt attatcggcg cttccttcac gatcgcgcgc gccagcgcta tcctttgctt
     2221 ctggcctccg gacaggccca ctcccctttc tccgattatc gtgtcataac cttccgggaa
     2281 ttccgttata aactcatggg cacaggcaag ttccgcagcc cactggacct tttcgaccgg
     2341 aacgtcggat gcgccgtaag cgatgtttcc ctcaatggtc tcggaaaaca ggaatatatc
     2401 ctgcatcgca accgctatgt tgtccctgag ggtcctgaca tcatagtcct ttacatccct
     2461 tccgtctatg agcacagtac cgcgggtaca gtcatagaat ctcgaaataa ggctcacgag
     2521 cgttgacttt ccggaccctg tcggtccgac tatggccaca gtctggcccg gattcgcgca
     2581 gaagcttata tcttccagta cgggctcctt tccgtatctg aagctcacat ccctgaattc
     2641 cacacgtcct tcaagctttt tagagacggc cccctctttg ctcctgatct ttggctccct
     2701 gttgaggagc gtcatgactt tttccgcgga agtggcaaat ctctgcgtat cgttgatcag
     2761 ccagcctatc atcctcatcg ggttgttcag cgcccagagg aaactgttga aagtgacgag
     2821 ttctcccagg gtgatgtttc cgttgatcac aaggataccg cccgccagga tgattatgac
     2881 cgagaggaat cctgccaccg catccatcac agggagatat ttctcccata tcttcgaagc
     2941 atccaggttg cagtccctga aaccggcatt ctcccttgaa aatttataga tttcgtagtc
     3001 ttcctgtgca aatgccttta cgaccctgtt cccgctgata ttttcctgga ccaccgaatt
     3061 gagcctggag aactgttccc ttatcgcgga aaatgcaggc ttgacagtgc ttgaaagcct
     3121 cacagcgaga atgcctgtta tcggagtgat agcaagaagg ataagggtga atgtggtatt
     3181 tatggtaaac agcacgatca gcaccaggat cagtgtaaca gcatgcatga aaacagaata
     3241 tatgacatag gcggtgaaat gcctgaccgc gtcaaggtcg ccggttatcc tggccattat
     3301 gtcgccggtc ttcgtacggt cgaaaaagtc gaaatccagt tcctgtatct tcctgaagac
     3361 atctctcctg gcattgtaca ccacattctg tgaaatgttc tcgaacatgt agaggtacgt
     3421 atacctgaaa atggccctca ggactgtgtt gcccaccatg ataagaacca gggtaagcag
     3481 cctgtcggtc tggccctttc caataacatc gtcaactatg atcccctgta cgaatgggtt
     3541 gatcaggttc agcaaattca ctgcaaatac aagtccgagg gcgacaaaga aaaggaacct
     3601 gtacttcctc atcagtcccc agacccattt gaattctctc atgtaaatca actgcctttt
     3661 tctatctcgt gttggatcgt ttccaacaat aattatcaac gatttgcttt gacaagaaaa
     3721 tgtacatttc tgcctttgtg atgtataatc ttataatgac gtattacgac ggccaattta
     3781 cattgcaggt tttgtcaaag gttggatgat ctatgtgtga tcttggcatt aagccggaaa
     3841 atttcaaccc gcaggtgtat tttactttta aaggcagata cgaagaaaac catgtgacca
     3901 aagtacattc ccatgatttc gtcacgatga tctatgtaat gtccgggaac tgcacgtaca
     3961 ccatcaacgg agcttcatac cgcgtaagga agggagatat gctcgttttt aacgccaacg
     4021 tgacacacgg caaaaacgtc ggcgccggag aagaaataat ggaactgcat gttggactcg
     4081 ggaatatatg tatcgacgga ttgccccgca accacctggt ggcttccgat gcgcctcccg
     4141 tttatcacct gcaggattat gagcagcaat tcatcaactg ctgcagcgat atctgcactg
     4201 agcaggaaaa caacctgccc ggatgcgaac tgatgataaa agtgcacgta atgaggatga
     4261 tggtgctttt catgaaagcc acgaaaacgg gcatccatcg cagtgcaaac acgctttcgg
     4321 gcatcgattc ctccgataag gcagtgatcg ttaataccct catgaaatat atgaacgaga
     4381 actacatgcg ccagatatcg ctcgaaacga tctcgaaaaa aatctacctt agtcctgcct
     4441 acatttcgaa ggtattcaag gatgagaccg gcgagactcc gataaattat cttataaaaa
     4501 tacgtctcgc caaagcgcgg gaaatgctga tggcaggcgg ccggtccgtc aagtccatcg
     4561 cccgggccgt cggctacgac gatgcatact acttcagcaa gctgtataag aaatatcacg
     4621 gcgtcgcccc gtcgaaggtg ttaaaggaaa ggggacactc cggtctttat tccacccagt
     4681 gcaatggttc gaagtaatcc gtgtagactc cgtaagcgcc attgtccctt accttgacat
     4741 actcattata gtcgtttaca gtatgtacaa atacaggaat gcccaaatca gagaattttt
     4801 tcagcgaatc ggtttttgcc cggctttgag acatcgtcag cccgaataat ggctgttcac
     4861 tgcagaattc aaaaacatcg tccccgatag tttcgatcct gtaaagtgtc aggataatat
     4921 gttcataacc aaggtttttg accggctcgt actcatcaag ggagtatatc tgaggaatga
     4981 tcttcttgct cgcttcggga taattattcc tgatataccc cagtacctcg acattgtttt
     5041 cctttatgtc agtgatgata tatgcatcat catgcatttc gagccacagc tcaagtttat
     5101 ccagatccat gaactcaagg cccaggatgc ccgtcctttt tttgaagtct tcatatccat
     5161 actcttcggt ggaatacttg atcccggcaa accagttggc gttgccccaa tcatgtatga
     5221 ataccggaat accgtcggaa gtgagactta tatccatttc tatataccga aagccttctt
     5281 catatgactt gttaagcgct tgcagggaat tagtcatacg gatgccgtaa atgtcgcctc
     5341 ctgcatgggc tacaagcctg ggcgccgccg gggttataac agcagggttg ctgccatgct
     5401 gttcatcgct gatgccatat ttattatcgg caatatcttg ctcgttgtca ctgcccttat
     5461 gttgaccggt atcttcatcg ttcggaaaat ttcctgtatt gtcggtattc aatgctgata
     5521 tactgtttcc tgtcctctgc agcacataat cgacagtccc gtgcagcttt cccggatcct
     5581 gatcctgcgc ttctgcctgt atctccacac cggcttgcac ctgggccccg gcctgttcct
     5641 ggccgggatc atggtcctgc gcctgattct gctgttgtat ctggctatta tccgggtttt
     5701 gaaacgtata tgagactgtt ctcatgcatg cagtcaacaa gaaaaaaaca tttacggtta
     5761 aaaggaccag taaagccttc ccccgtctca ttctccactc accccaacct tagcgctttc
     5821 aaggcaaaca tatattttaa gcttaccctt tgcgtttcgt atcgtcaata ttttatgctt
     5881 gactgaaaat ataataagaa gtatttttgt aacgatatgg tataatatcc tttacatgaa
     5941 tatattggga gagtagggtc aagtgagcat aacagaggcg cagttaaaag ctttctgttc
     6001 aggtacatca tcccctcttt ccttttaccg gctgttgttc tcgtgctgat catggggata
     6061 aaagtgatcc ctgatgtaat attggtattt atcaatatga ttgtcctggt tctgctcgta
     6121 ttcaagatgt caaaaaaaga gctcccgttc tgcaggagct ttcagcacac agttgatggg
     6181 aacaccgggg gagtggtatt cctgtcattc ttcctgatag catttatggc ggggatccac
     6241 ttcgcgctgt catttgcccc gccttttggt gtgattgcaa atatcgccgt ttcagtgctt
     6301 ctcagtgttt tattgtggag aagctgcttc aggatctcgt ggaaggatgt agaatattaa
     6361 aaagtacatt gcgtactgga aaatcagttg cgcactgcat cacttttgcg gcaggagccc
     6421 gatgatatag ttggtaagct ccttcgctgc tttttcatgg gcatgtttgc ccgggtggga
     6481 tctcgatccc attgtttcgt ttgtagtgtt gggaagctgg aatatggaca ccttcctgtc
     6541 tccggtgctg cggatatatg tatcggccgc ccggtagatc gacggcatca ggggtgcgcc
     6601 gaacattccg taagcccaca ctatatgcgc gtttttattg tactttctca gtttcccgag
     6661 gaaacctgca gccgcgtttt caaatgccgc cagatcttcc tcattgaatg tgccgtcctc
     6721 attcagcctc tgcttatgcc gggctccgct tgcctcatcg acccattccg gtgtgctgaa
     6781 cgcattagca tcgttagttc caaggttgac aactatcaca tccggctgcc attcgtcaaa
     6841 atcgttctgc ccaaatgcat ccgccacagc acagttctct ttcggcaata ttccgcagat
     6901 ctgctcatag taacggggta tgctgcagtt cggattattg tcccagccgg tcaggacgcc
     6961 ccatccgctt tgggaaacga ccctgtagtc cgcattcagc gcttttgccg tcagataggt
     7021 gtaattgtcc gccgcgctga accacatggg gatccagtcc tgttcgcatg atgccccgac
     7081 agtcccttcc cctgatgtta tgctgtcgcc tatgaactcg attttgtacg gcctgtcctc
     7141 aacgggaagg aattccccgt caaatctcac agaatgtata tgtaccaggc atcctttgtc
     7201 aaagctcatt ggctggacat ccttcattat tcgcacattc tttgcggcgc ccgcgttcat
     7261 ccctctgaat acgcataacc aatgtcttcc cgcagtcacc atctgccttg ctacaggata
     7321 tgagttgatg agtatgctta tccacggctc atacacgtca tagtccgtct cgacctcgat
     7381 ccacagttcc gaacctttga cattgaactc gacgccgctt ccggtccaga aaagcgttaa
     7441 cggtgaaagg cgcccggtca tgcgcccgtg gaccttcatg tttcggatat cagataatga
     7501 atagatcttc agattgctgt tttctttcat ttgaacacca ccctaactat tggttatctc
     7561 atatgtcctg tgttcattca ggaaaaggaa ctcttcatct tttaagtcaa atctgtcgct
     7621 taataatatt tataaggcat aactgcagtc acttatgata cgtctcaccg ttgatgatct
     7681 tgaatgcccg gtatatctgc tcgagcaata taaggcgggt cagttgatga ggaaatgtca
     7741 tgtcagacag ggacagcctc acgttagctt ttcttataag ttcaggagca agtcccaggg
     7801 aaccccctat aacaaaggta atactggagt tcccgcttat catacagttt ttcagcacct
     7861 ttgcaaactc ttcggaactc ttttttgcac cctttacgtc caggactatg aggtaatctc
     7921 cgtccttcac tttgccaaga actctggatg cttccctgct tttgatctgt tcctcccggg
     7981 ctgcgcttaa atcatcaggc gccggttcat ccgctgcttc aatgatcctg agatcgcaga
     8041 accgggacag gcgttttgaa tactcctcaa tgccttcctt caaatagtgt tctttgatct
     8101 ttccgactgc tatgatggta accttcatat gatttttcca cgtaaacttt cttatttata
     8161 atctatccaa aaaccatctt ttacacggca ctttccctgt gaatcttcca aacagccaga
     8221 ctgttctcaa tgtcaccggg ccagcctgcc tgtttccgca aaaaaatact tcataattcc
     8281 gttatttcgc aggactgtcc ccaataaatc ctgatttcca ggagcaagcc caattctgca
     8341 gaaccgtccc ccagttttgc agaactgtcc tccaaaaccg tgccgtcaga tctcgatcac
     8401 tctgcttgcg cggtcacgga gggctacgtc aagcgtgatg tcagcgccgg catttacgca
     8461 cttctcacac agtacgctgc gtacagtctg gtaggccagt tccgggaagt tgttctcctt
     8521 gctcaggtgc ccgagcagga acttttttgt gccgcgctcc gccatatagg caactacttc
     8581 gccggcggcg tcattggaaa gatggccgtg cttgcctgcg atgcgctgtt tcagcggcca
     8641 cggataaggc cccattttaa gcatttccag gtcatggttg gattccagca gcaacaggtc
     8701 gctgtcctcg aaattgcgga gcagatccat gctgatatgg ccgatatcgg tagcaatggt
     8761 agctttcctg cccgctgaag aaaagctgta gcccaccggc tcattggcat cgtgcggtat
     8821 cgggaacggg gtaatggtaa tgtcccctat ttcaaaaggc acatatgaag agaatatttt
     8881 tttattacac tcacggacag ggccgatcat ctgatccatc gcttcccatg tgccccggct
     8941 cgcatatatg ggcaggtcgt actttcgcga caggacgcct gcgcccctga tatgatcgat
     9001 gtgttcgtgg gatactaaaa tggcgctgat ttccgacagt gattctccta tcgaacacat
     9061 cgccccggct atctttttac cgctcaatcc cgcttcgacg agcagcttcg ttctctctgc
     9121 cgctatgaat acggcattgc cgctgctgcc gctgaataaa ctgcaaattt tcatccctgt
     9181 atcaccgtaa taaagcactg cagcccaaag tgctgctttt cttcagtaat gacgccaata
     9241 tagttattct tcaatcctta cgatatctgc cccgaggctc tgaagcttta cgacgaagtt
     9301 ttcataaccc cggtctatgt actttacatc gcttacctct gtccttccct cagcagcgag
     9361 gcctgcaagc acgaccgctg ccccggcccg caggtcgatg gcattgacct gggcgcccgt
     9421 taacgtcacg ggaccttcaa taatagccat gccgccgttt gcgcgtatct tcgcacccat
     9481 cctgcgcagc tcgtccacgt actggaacct ttgcggccat atgctttccg taatggtgct
     9541 gttcccgtcc gcgagacaca gtagtacggt taccggcgga tggagatcgg tcgcaaaacc
     9601 aggataggga agcgttttta tattcacctt ccggatagtg ccgttgccgc ccgtcaccct
     9661 tatccagtca tctccctctt caacattgat gttcatttcc tgaagcttcg ccgagaggga
     9721 ttccagatgc ttcggtatga cattccttac ggtcacgtcg ccgtcggtgg cggctgatgc
     9781 tatcataaac gttcccgcct cgatcatatc cggtattatg gagtggacgt tgccgccttt
     9841 aagggacttt actcctctta tctttatgac atcggtacct gcgcccttta tatccgctcc
     9901 ggcagcattg aggaaattgg ccgtatccac tatatgaggt tcctttgcgg cattttcgat
     9961 aacagtggta ccttcggcca tgactgccgc cagcatgaga ttgatcgtcg ctccgacact
    10021 gataacgtca aggtatatct ggttccctgt cagtttctcg gcagaaagtt ttatcattcc
    10081 attttcaatg acgaccttcg cacctaaagc ctcaaaacct tttatatgct gatctatcgg
    10141 cctgtgaccg aaatcgcagc cgccgggcaa agctacttcc gcttttccga aacggccgag
    10201 cagtgccccc aaaagataat atgaagccct catgcttttt atcatgtcat aggatgctat
    10261 ataggaattt acatcggtgg aatcgatgag aactgtgttc ctgtcttcga atgtgacctt
    10321 tgctccaagc tgggtaagcg tttcctccaa tatgcgggtg tcttttacat caggaagatt
    10381 ttctatcctg cagcttcctt cgatcagcaa tgccgcaggc aatacagcta ccgctgcgtt
    10441 tttggcaccg cttatataga tatcgccttt cagcggcttg cgtccgtgta tgatgatttt
    10501 ctccaatccc tgcacacctg ctttcttgtc attcgtgtaa aaaggcagaa tatgcccttg
    10561 ttccctgtta cattatatca ctgtttcatg caatactaca acggcatttt gatgcgcggg
    10621 cttcagccac cggattacag gcggtggatg caagctaaca ggtcctatgt gccagataag
    10681 agaagcagat atagaataac aggtaccctg cgccaggcgt cggactgcag gcaaaatgtg
    10741 ccgagtgcag gccggcagat atcatgcggc aggcgtcagg cagcaggtac catgtaccaa
    10801 gtatcataca tcccgcggca agcgccatgt acctggtata gagcgacaag cgtcatatgc
    10861 caggcatcat gctcaggtgt catgcggact gatatacaca ggctcatgca ttccctttat
    10921 tttgacacgc cagactggca gtagctctac tgtttccaca tctcccatcg atggctcctc
    10981 cagcttgtat ccgctgtcta tggatgtgat gaccatgtcg cttttttctt taaaataggc
    11041 aagcaatgcc tgataggcct cgaactgttc gatattttca tttgagaatc ctttgaattg
    11101 atgtctccgg tattcgagac ggcttacctc cctgcccctg agcgtgatgg aaaagcaatt
    11161 gtcgaacagc agcgatccgc catacgtttc tatgtaatcc agtgtccagc cgccgtcacc
    11221 gttctcttct gaacggtcca gcacatactt tccggcaagc agtccttttt ccttcatgaa
    11281 tttcattgca gcatcttccg cttccttaac gctgacagca tcctgggccc gtgcttcggt
    11341 tccggcggta tatatgaaac tgtcaatgcc ggtgaattcg atcttcccgt tgctgctgaa
    11401 aaaatgagaa ccgtccccgg aagcttcata cttcgttccc aaaagccttt cggcgatatt
    11461 tcctccgtcc agttcacttt ttatgtacac aagcctgctg ttgccgcccg gcttcgaagg
    11521 gatctcacat tcaagcctga tacccctttg ctccagtatc agcctggtgt tttccagggt
    11581 ctccttgctg acgccctggc tgctgcggaa tatcagcatg tttgcaagca gaaaaacgtt
    11641 gaaaaccagc agcaggacaa taactacgtt tttagctttt gatacatcca tatatcaatc
    11701 tccttcttcg gcgatcatat caagataagc cgtggcccct tcccccgtcg ttattatcag
    11761 cgcaggctca agtaacttat ccttgctgct gaggatataa tagcctgtat ctatccttga
    11821 tatgtttata ttgcgccaca ggatgttttc atttgataaa agctccatca gcctgtcatt
    11881 ataattgtgc gtacgcccga tcctgaaact tctgatcagc cagtcgcatt ccagtacccg
    11941 tctgctgtcg gcaagtatcc ttatcgcata gcccagttta ccgccgtttc cgtcctttgt
    12001 ttcatattcc gtcctgactt tcaggccgta tacacggtag tcgaagccga actcatatat
    12061 gccgccggct tttttctcga cagacgataa gattatatct gtttccggcc ctaccaattc
    12121 gtttattccg gccatgaatt tatatgcgtt catcagcgct tcgcttgcct ttgtcccgct
    12181 cgtgtctgcg ctgccgagat accggtatgt aagatatccg tctgaataat acctgtaaat
    12241 gttgttccca tacgtatact ggatcatatc tccgttatat gtatacttgt tgtacctgcc
    12301 ttcttcatct ccaagtatga tatttgccag catatcttca tcatttgccg ccgccggcag
    12361 atccgccgac aggaccctgt aaggccagta cctcggcgaa gaaactacat aaaggacatc
    12421 aggctcatcc ccggacttat tcagcttgct tccggacagg gtcttgtatt tacggtactt
    12481 attcttgctg ccttcttcaa gtttgcttat tacgttcgta agattggcag cctgttcata
    12541 cctgacctgg tccgtactgt ataccgtacc gtcatcaccg cagatgtaga atgtgccggt
    12601 atcctcgcgc actatatcct ttcttaccat aagtttgctg aatcgtggca tatcctgcac
    12661 aggacttccc atcccgagga accatcccag cagatcgggc tccagataaa atccgaaatc
    12721 gacaaggata cccctctttg taactatttc ttcccatttc tcatctgaca cgttcagttc
    12781 tgcgttaccg gtcgcgatcc tgctcagccc tgcgatcact tcattgaaaa atttcatgta
    12841 atggtcatgg tcatttgaga ttatccagta atcattatct ccattggaaa cgacaagctt
    12901 gtcaggtacg aacagattct cccttatggc ttcattgctg acctggatat ctttgctgaa
    12961 aagtgccgat aaaaaactaa aaggagtccc ctggttctga tagttccaaa ggatccccac
    13021 ctgtaaaaga cctgttatta tcaagataaa aataaggacc gtcttcagtc gttctttcaa
    13081 ttcacggctc atttaagtcc tcgctgttat gtagtttact tactgccgaa ccagtcaatg
    13141 atctgcttcc ctgtcttctt caactgggcc caccagctgt ctttgaaaac ggtaaccgca
    13201 atcggggtat actggatgtg ttccgggtcc ttctcggaag tcctgtatgc tatgagaagc
    13261 aagtcgtatt cgccggggtc aagttcgagt tcggtcgata atgcaccgga accgatatta
    13321 agcactctgt ctccgtcggg aagttcgatc gtctcataca cgcctgtctc actgttcagc
    13381 atcgctatca atatctcaac atccgtatat tctgattcga aggagatttc aaaagttttt
    13441 ccatatgtgg tgacatcccc ttcgatattc tcgggcttct ttatggttac gacagactgc
    13501 ccgttgctta caggcttgcc tgcttccagc acttttctga tagcgcgcca gaatgcggcg
    13561 tcgggatctt ccttctgctc cttatccttc tcagtacccc cggaagtttc tttttccgcg
    13621 gaagcggcat ttgttttggt ttcaccggtg ccggtggtct taaaagcaac atttgatccg
    13681 gtctggcttc cgccgtcctt ctgggtgctg ttgtttgaag gcagagacgc aggtatattt
    13741 gaaggcgcga caattgaatt tgcaggtaat gccgcagctg cattcgaagt cgctgacgta
    13801 cccgacggtg catttacggt cgtttttgaa gatgtgcccg tattaccttc ttcaggtttt
    13861 gcatatgctg cagatacggc tactatgaat atcagcagca ttatgaaaac aacactcagt
    13921 tttttcagca ttgaccatat ctcccaaact gctcagccgt catcggccgc agataaatgc
    13981 attttagcta gctttattat acagaaatag tgttacagaa atattacatg ccatttaaga
    14041 ttaaattaca taaaaatccc tcaaaaagcg ctattttact ggcttgtctg cctcatggga
    14101 agcctgaccg taacttccgt accagcgccg actttactgc ttatgttaat cgatccatta
    14161 tgggcttcca cgatctcctt cgctatggaa aggccgagac ccgtgcctcc catttccctg
    14221 gaccgggcct tgtccaccct gtagaacctt tcgaatatcc tgtcgatatc ctcttcaggt
    14281 atgcctatgc cagtgtccac gaccttgaaa tatac
//
LOCUS       DAOSCC010000033        14225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_2895_length_14225_cov_4.291249, whole genome shotgun sequence.
ACCESSION   DAOSCC010000033 DAOSCC010000000
VERSION     DAOSCC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_14225_cov_4.291249"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLG72_03150"
     CDS             <1..728
                     /locus_tag="PLG72_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ79830.1"
                     /translation="GRQPKLYKVSGVGILEPSQDEKSYAVFVNKDYLKKIIKENQRAQ
                     GGRGSSSMDDGYWQVKVKVKDMKDVERVQEEIKSMGYGTYSLLDILKSMQETAATMQT
                     VLGGIGAISLLIAAIGITNTMVMSIYERTREIGVMKVLGCMLGDIRNLFLFEAALIGF
                     IGGLLGIGFSYGVSVLLNRYAGGLFGGGYYYGDPSESIVSLIPPWLALAALVFATLVG
                     IISGFMPARRAMKLSALEAIRTE"
     gene            complement(888..2552)
                     /locus_tag="PLG72_03155"
     CDS             complement(888..2552)
                     /locus_tag="PLG72_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ79831.1"
                     /translation="MLDKFLPRQDFSSYEDFRENYRVNLPDNFNFAYDVVDEWAARQP
                     GKTAMVWCNDHGCERIFTFADMKRLSDRGANFFRKYGIKKGDTVMLLLKRHWLYWVCT
                     LALEKIGAISIPATIQLTKKDIIYRCNAASVKMIISAIDHEIVSYIEEAAAESPTVMY
                     KALVGEKREGWIDIETEIENAPDEWTRPVGSEDAGGRDNMLMYFTSGTTGMPKIVVHD
                     FYYPLGHIVTARYWQRLGSEGLHLTVSETGWAKCAWGKIYGQWICGVAVFVYDMDKFV
                     PVNLLKMMEKYRITSFCAPPTIYRFLIREDLSKYDLSSIKTACTAGEPLNPEVYNQFK
                     NATGLDIIEGFGQTEATVLCANFEWIKPKPGSMGKPSPLYNIDIVDDNGNTCPPGVEG
                     RIVIKGLDREVPPGLFKGYYRDEESTSRVWHDGMYDTGDVAWRDEDGYFWFVGRSDDV
                     IKCSGYRIGPFEVESALMEHPSVLECAVTAVPDPVRGQVVKATIVPAKGWHPSDELIK
                     ELQDHVKRVTAPYKYPRIIEFVDELPKTISGKIKRKEIRQHDLDRY"
     gene            complement(2593..3159)
                     /locus_tag="PLG72_03160"
     CDS             complement(2593..3159)
                     /locus_tag="PLG72_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ79832.1"
                     /translation="MSEKIKEMADRIREIREVSGISAESLAKKLNIPTDLYKRYENAE
                     EGVPISTLYQIAGILKVDMTELLTGSTPRLQSYCLVKNGEAPFIERYKGYKFQSLAYS
                     FMNRKIEPLLVTIDPEENNKNMSLVTHPGQEFNFALEGRIKVILGGKEIILEPGDSLY
                     FDPTIPHGQTAADDKPCRFLTVILNDKL"
     gene            3347..3625
                     /locus_tag="PLG72_03165"
     CDS             3347..3625
                     /locus_tag="PLG72_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79833.1"
                     /translation="MSEHERRLLKMARKGGVKAFEELTAQHQRNVYNYFLINCANEYE
                     ACRLTQNVFVKVFEKLTSNAIESDLFSYIYRTAGEILRQQDSASKMIS"
     gene            3787..4485
                     /locus_tag="PLG72_03170"
     CDS             3787..4485
                     /locus_tag="PLG72_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ79834.1"
                     /translation="MSEAKTKVLVVEDEAPIRKFIAINLERSGFTVLEAETGEKGLEM
                     ARAYKPEIMVLDVMLPGIDGFEVCKKLRDEMPELIIIMLTARGQDIDRIAGLEIGADD
                     YMVKPFNPRELTARINSVLRRIERIKMPDEEPLVYKNLVMDLKSQKFYKDNAEIELTP
                     TEFAVLKMFMSNVGRALSRNELFNAVWGKNYFGDLKTLDVYIRRIREKIEDNPSKPKY
                     IETVWGYGYRWCDR"
     gene            4508..5914
                     /locus_tag="PLG72_03175"
     CDS             4508..5914
                     /locus_tag="PLG72_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ79835.1"
                     /translation="MSLGIRSRMTFGFIVIILLTILVMEALIINIVRVNYYNNLEAGL
                     YNQLKIACEMYSRYFSDASLSENVMNNVDTFWRQTSAQVQIIDTDGRVLMDSIGYMPE
                     DDEKMPDVEKALETGRGSWTGKVPYDSYGVMAVSASLTADGKTVGVLRFISSLRGVDE
                     DVRSVSRIFIVIGAVVAVMSMLLSLLLSSTIVNPLKKVTYAAARMASGDIHARSVKTY
                     NDEIGKLSDTLNYLAEEVEKRDRMKNEFISSISHELRTPLTSIKGWAVTLKEVGSEDR
                     ELHENGLDIIENECDRLTAMVEELLDFSRLVSGKLMIKRENVQLEEFVDTVGRQLAPR
                     AARDGLHFSVEMEGELPEIVTDGNRLKQVIINVLDNAFRFTPAGGSVLFKTVASGGEV
                     IFTVSDTGCGIPEDELPYIKEKFFKGRNSKLGSGLGLSICDEIIKMMGGSINIYSREG
                     KGTKVVIRIPCIMEGGAA"
     gene            5911..7494
                     /locus_tag="PLG72_03180"
     CDS             5911..7494
                     /locus_tag="PLG72_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79836.1"
                     /translation="MMKKQHCGRLRRIFMMLLALALTLSACTQAVPESTDRIISPVNM
                     AVPAAGTWVLERRLTDEITDISDADDSLTGEKVGFTQNTMFYAGQVFTDISYKIKRVN
                     VYEYFLHKNRDILKKLNYENNEILVVNVYSKDSFLFEFIIDSNDEKIAVIDDRLYVMR
                     KVSDDVSGEQEITAHDTPESDMEDAIAQKQPLRSGLLMGVRYPVQTSDGLGDYKYGTY
                     WISCINGAINPVLYADAIYLPRMDGFWKLMVEKRPGPSGLVDTLRAFKVTNTSNKAVD
                     RPSAASPENVSERMETRVRRAIIYVGNDYACVENTIYGTDSDNAGAIYEKNLRTLPVD
                     NLMNVDGIKISDMAGENGTMAMENAITEVLVNSGYEGIVILDDDEQQKNFALYRKTGH
                     WFFKGRIDPDRQGNLPYMDFNLNLIPPSNMVAYDVLHVPWKEMKDKLPNAIDIYTSPN
                     RDLAVILTRSHILLYAIEGSKLADEPLAKIQLEEGSAVIMAEWCMGEYVSRWEQSFIR
                     NNDTREVFDGTGDSEAVQD"
     gene            7804..8247
                     /locus_tag="PLG72_03185"
     CDS             7804..8247
                     /locus_tag="PLG72_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ79837.1"
                     /translation="MESKSMYLLVDSAILPEVFAKVIEAKKLLSSGKAKTVNDAVSAA
                     GLSRSAFYKYRDHVFPFYETSRGRVITLYFAVEDFSGILSRIINKIAEARANIITINQ
                     NVPINGLADVTISISTAGMTMDLDKLMEGIAEIEGVRKQQILARD"
     gene            8263..9510
                     /locus_tag="PLG72_03190"
     CDS             8263..9510
                     /locus_tag="PLG72_03190"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ79838.1"
                     /translation="MMDIAILGYGVVGSGVAEVIRKNGQSIADRAGEKIKVRKILDIR
                     DFPDSPDRDLMTKDPEEVFGDPDIKVVVETIGGTGVAREYTKRAFNAGKHVVTSNKEL
                     VAVHGPELLKLAADNGVSYLFEASVGGGIPIIRPLSQCLAANIINGITGILNGTTNYI
                     LTKMRRENCSFDEALKNAQLNGYAEADPTADIEGHDTCRKIAILSSIAYNEYVDCSRI
                     HTEGISRITLTDMLYADAMDSVIKLIAVSRREGDRVFARVSPAVVSREHPLAGVEDVF
                     NAIVVSGDAIGDAMFYGRGAGKLPTASAVVADVIDIVKHNESTRSVWQRKETDNMVPF
                     GESVTRCLVRVAVKNRNEAAADIGKIFGGVEYVSADIKGIDDELAFTTGKMTESSLSE
                     RVGELKSMRSVKEVVNVIRILEQ"
     regulatory      9634..9830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10078..10605
                     /locus_tag="PLG72_03195"
     CDS             10078..10605
                     /locus_tag="PLG72_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOJ79839.1"
                     /translation="MKGLIHIYTGDGKGKTTAAIGLGVRACGRGMKVMMAQFLKSAPT
                     GEVYSLKSLEPDFVLCRGTGSKKFTWQMDESEKVQAAAEQQAIFNYAVNAAQNGECDL
                     LILDEVLGAITTGMIEKEQLVSFIRNKPDRLELVLTGRNADPELIGLADYVSEIMAVK
                     HPAEKGIHARKGIEF"
     gene            10658..11890
                     /locus_tag="PLG72_03200"
     CDS             10658..11890
                     /locus_tag="PLG72_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ79840.1"
                     /translation="MVFMKSFAKNADINPRQERKTADCGKKINLIGRFAVRRGLVLLV
                     AVIMLLAVLAGCSGQDRAGSISDNAGLQSGAGSEDAVAGNGTAGASGDGSGSISGGSF
                     DTSGGSETGAGGDGTAAGTNASSGFPLTVTDANGDVMILENPPKRIISLTLGSDEILL
                     DLVDKSRIVSLTRYADDEGISNIAAEARGVGVRTTMDQIENIIALEPDLVITDTWADV
                     NYIKQLRDSGITVYAFRTPNNIDEQKAVIAELAHVVGADEKGQELINWMDGILKAVEE
                     RLSVLEPGQKLTVMDYGELGSSGKGTNFDDIVTRAGLVNVISRAGIEGWPVISKEKII
                     EFDPDIIILPSWYYDPGNTFESMRDSLVNDPSLQTVSAIKNNRIISVPNPHISAISHY
                     VVLAVEDVAKAAYPELFE"
     gene            11918..12967
                     /locus_tag="PLG72_03205"
     CDS             11918..12967
                     /locus_tag="PLG72_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ79841.1"
                     /translation="MLKTGNLTVYKKMSVILPVLVALLAFMIILAVALGAVYVPFGRS
                     FRIILDNIGLIDDPTLPAEQVSIIYMVRLPRVIVACVAGAALSACGAVMQGLFRNPMA
                     DPGVLGVSSGAGLGAIISITTGLAARSIYTMPMFAAVGALGAVAVIYLLSLRRGRIAP
                     LTLILSGIAVSTFIGAVTQFILIKSNTYEVRRYVFWTMGGLSGMMWEQVRLVTFPVLV
                     LLAALMMFSRDLNLLQLGDEEAQSLGLEPSRTRKLLLLITSLITAVTISVCGPVSFVG
                     LIVPHIMRLIAGPDHRLLIPASALGGAIFLTGCDILGRMPANGEIHVGIITAMLGAPY
                     FLYLLIRTRKGGAAF"
     gene            12964..>14225
                     /locus_tag="PLG72_03210"
     CDS             12964..>14225
                     /locus_tag="PLG72_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79842.1"
                     /translation="MNGERSGYDGGRSGSSGQDSKLLRVDNLNYHTDGVHILKDISLE
                     AEKGEFIGLIGPNGAGKTTLLKCINGINRGTGLVELGGRDIRTMSSRQIASMVAMLHQ
                     DTAVAFPFTVLDVVLTGRYHKLGRFRPETPEDYAVARRCMEYTDTLRLESRAINTLSG
                     GERQRALFARVLAQETDLILLDEPSASLDIAYEEQLFKNARELGEGGKAVIAAVHDLK
                     IAVRYCTRLIMMKDGRIIADGSPESVITRENLRQSYGVDAIVYRNRVTGLVDFHISGE
                     SRSRKDISVHVIGGGGSASGVIRQLFELGCNITAGVFSHGDSDLACAGIFGVRTVTCR
                     PFSEIDDDSFRENVELVKASDMTILCDMPFGSQNIRNLEAARHASELVIIEDSDPQSR
                     DYTGGRALEIYNELKKRAVVVESARLHEV"
BASE COUNT         3721 a         3364 c         3990 g         3150 t
ORIGIN      
        1 gcggcaggca gccaaaactg tacaaagtca gcggcgtcgg tatactggaa ccttcccagg
       61 atgaaaaatc ctacgcggta ttcgttaaca aggattatct gaagaaaatc ataaaggaaa
      121 accaacgggc ccagggcgga cgtggctcca gcagtatgga cgatggctac tggcaggtaa
      181 aggtcaaggt aaaagatatg aaggatgttg aaagggtaca ggaggaaatc aagagtatgg
      241 gctatggtac ctacagcctg ctcgacatcc tgaaaagcat gcaggaaacg gcggcgacga
      301 tgcagaccgt actcggcggc attggagcaa tatcgctgtt gatagcagcg attggtatca
      361 cgaacaccat ggtcatgtcc atatatgaaa ggacaaggga aatcggcgtc atgaaagttc
      421 tcggctgcat gctcggcgac ataaggaacc tgttcctctt tgaagctgcg ctgatagggt
      481 ttataggcgg cttactcggt ataggcttca gctacggcgt ttcagtgctc ctgaaccgct
      541 atgcgggagg cctgttcgga ggcggatact attatggtga tccttcggaa agcatcgttt
      601 cgctgatacc gccatggctt gccctggcag cactggtctt tgcaacgctc gtcggcatca
      661 tatcaggctt catgccggca aggcgtgcaa tgaagcttag cgcgctggag gctatcagga
      721 cagagtaagg ataaaactca ggcaggacaa gtgcacacga aaaacgaaaa agagaatcag
      781 gataagtaat aaatgcggaa aagggggaca cgaatccccc ttttccatac ttgattgaac
      841 atcatcggta catcatcagg cttaaataag actgtatcag tatttcatca atacctgtca
      901 agatcgtgct gcctgatttc ctttctcttt atcttgccgc ttatcgtctt gggaagctcg
      961 tccacaaatt cgatgatcct cgggtattta tacggagcag tcactctctt tacgtggtcc
     1021 tgcagttcct ttatgagttc gtcgctggga tgccatccct ttgccggcac gatggtcgct
     1081 ttcacgacct gtcccctgac aggatcaggc actgccgtga ccgcgcattc gaggactgac
     1141 ggatgctcca tcagcgcgct ttcaacttcg aaggggccga tcctgtagcc ggaacatttt
     1201 attacatcgt ccgagcgccc gacgaaccag aaatacccgt cttcatcccg ccatgccacg
     1261 tccccggtgt catacatgcc gtcgtgccag actcgtgaag tagattcctc atccctgtaa
     1321 taacctttga acagcccggg aggaacttcc ctgtccaggc cttttatcac tatcctgcct
     1381 tcgacgcctg gaggacaggt gttgccgttg tcatccacta tgtcgatatt gtaaagcgga
     1441 gacggtttgc ccattgagcc gggcttcggt tttatccatt caaagttggc acagagcacg
     1501 gttgcctctg tctggccgaa accttctatt atatccagtc cggtcgcatt tttgaactgg
     1561 ttgtacactt cggggttcaa cggctctccg gccgtacacg ctgtttttat gcttgaaagg
     1621 tcgtatttcg agaggtcttc cctgatcagg aagcggtata tggtcggcgg cgcgcaaaaa
     1681 gaagtgatcc tgtacttttc catcattttg agaagattca caggtacgaa cttgtccatg
     1741 tcataaacaa atactgcgac accgcagatc cactggccgt atatcttgcc ccatgcgcat
     1801 tttgcccatc ctgtctcgga taccgtaaga tgcagacctt cgcttccgag gcgctgccag
     1861 taacgggcgg tcacgatatg gcccagcggg taatagaagt cgtgtacgac tatcttgggc
     1921 atgcccgtgg tcccggatgt gaaatacatc agcatattgt cccggcctcc ggcatcttcg
     1981 cttccgacag gccttgtcca ctcatcaggc gcattttcaa tttcggtttc gatatctatc
     2041 cagccttctc ttttttcgcc caccagggcc ttgtacatga cggtggggga ttcggccgcc
     2101 gcttcctcta tgtacgagac gatctcgtgg tctattgctg aaatgatcat tttgacagag
     2161 gctgcgttac acctgtatat gatatctttt ttggtaagct ggatcgttgc agggatagat
     2221 atggctccta tcttttccag ggccagcgta catacccagt aaagccagtg ccttttgagc
     2281 agaagcatta cggtgtcgcc ctttttgatc ccgtactttc tgaaaaagtt ggcgcccctg
     2341 tcgctgagcc tcttcatgtc ggcaaatgtg aaaatacgct cgcagccgtg atcgttgcac
     2401 cataccattg cagtcttgcc gggctgtcgg gcggcccatt cgtctacgac atcataagcg
     2461 aaattgaagt tgtcaggaag atttaccctg tagttttccc tgaaatcttc atatgagctg
     2521 aaatcctgtc tgggcaaaaa tttatcaagc atttgtatct gaactccttt tgtttatata
     2581 ctcatgtctc tttcacagct tatcgttaag gatgaccgtc aggaacctgc acggcttgtc
     2641 gtccgccgcg gtctggccgt gggggatggt aggatcgaaa tacagcgaat caccgggttc
     2701 aagtattatc tccttgcctc cgagtatgac ctttatccgg ccttcaaggg caaagttgaa
     2761 ctcctgcccg ggatgggtca caagagacat atttttgtta ttttcctccg ggtctatcgt
     2821 aacgagaagc ggttcgatct tcctgttcat gaaactgtac gcgaggctct ggaacttgta
     2881 tcccttgtaa cgctctatga acggggcctc accgtttttg acaaggcaat agctctgcag
     2941 ccggggcgtg cttcctgtca gcagttccgt catatctacc ttcaggatgc ccgctatctg
     3001 gtacagcgtc gatatgggca cgccttcctc ggcgttttca taacgcttgt acaagtccgt
     3061 cggaatattg agctttttgg ccagagattc ggccgatatc ccggaaactt ctcttatctc
     3121 cctgatacga tccgccattt cttttatctt ttctgacaaa tctatccctc ccagcgcatt
     3181 ttgagacatt gttttttgca ttaataccta taataacaca atatagccgc ggaattaaac
     3241 aaaattttaa cgcatcattt gaattgtaat aaatgaaaat tttaaccaat tattaatcta
     3301 ttgtttttga taaaatcgta tgattattta tggaggatgc gggattatga gcgaacacga
     3361 aagacgactc ctgaaaatgg ccagaaaagg cggcgtcaaa gcatttgaag agctgacggc
     3421 tcaacatcag cgcaatgttt acaattactt tctgatcaac tgcgcaaatg aatatgaggc
     3481 atgccggctt acacagaacg tctttgtcaa agttttcgaa aaacttacct caaacgccat
     3541 tgaaagcgat ctgttttcat atatataccg gacggccgga gagatcctcc ggcagcagga
     3601 cagcgcatcc aaaatgattt cctgaaccgg cggtcctggc atccgggaac cgcggacaat
     3661 gttccagttt ctgcaaaatc tgtataaaac ggagttatcg acgatccaat ggcacaagag
     3721 ccaaaaatat tgtagaataa gggtgtcact ttgtttatct gcaatcacgc aaacggggtc
     3781 atcataatga gcgaggcaaa aacaaaggta ctggtagttg aagatgaagc gccgatcaga
     3841 aaattcatag caataaatct tgaaagaagc ggctttacgg tgcttgaggc cgaaacaggc
     3901 gaaaaggggc tggaaatggc cagggcatac aagccggaga taatggtgct tgatgtaatg
     3961 cttcccggta tcgacggctt tgaggtgtgc aaaaagctcc gtgacgagat gccggagctc
     4021 attattatca tgctgaccgc caggggccag gatatcgaca ggatcgcggg ccttgaaata
     4081 ggcgccgatg attacatggt aaagccgttc aacccgaggg aactgacggc aaggataaac
     4141 agcgtgctca gaaggatcga gcgtataaag atgcctgacg aggagccgct tgtctataaa
     4201 aatctcgtaa tggacctgaa gtcccagaag ttttacaagg acaatgcgga gatagagctg
     4261 acaccgacgg aattcgccgt tctgaagatg ttcatgtcga atgtcggaag ggcgctgagc
     4321 cgaaacgaac ttttcaacgc cgtatggggg aagaactatt tcggcgacct gaaaacgctg
     4381 gacgtttaca taagaaggat cagggagaaa atagaggaca atccttcgaa gcccaaatat
     4441 atcgagaccg tatggggata cggctaccga tggtgcgaca gatgacggat cccggggaga
     4501 atccagcatg agtcttggta tcaggagcag gatgacattt ggtttcatag tgatcatatt
     4561 gctgacgatc cttgtgatgg aagcgctcat catcaacatc gtacgcgtca actattacaa
     4621 caacctggaa gcgggcctct acaaccagct gaagatcgcg tgcgagatgt attcaaggta
     4681 tttttccgat gcttcccttt ccgaaaatgt aatgaacaac gtggatacgt tctggagaca
     4741 aacgtcggcg caggtccaga tcatcgacac cgacggcagg gtcctcatgg attccatcgg
     4801 ttacatgcct gaagatgatg agaagatgcc cgatgtggag aaagccctcg aaacgggaag
     4861 gggttcatgg accgggaaag tgccatacga cagctacggt gtgatggccg tgtcggcttc
     4921 gcttacggca gacggcaaaa cagtcggggt gctgcgcttc ataagttcgc tcaggggtgt
     4981 ggacgaggac gtaaggtcgg tctcaaggat cttcatagta ataggggccg tggtggcggt
     5041 catgtccatg ctgctgagcc tgctgctttc cagcacgatc gtaaatccgc tgaaaaaggt
     5101 gacatatgcg gctgcccgaa tggcatccgg agatattcat gccaggagcg tcaaaacata
     5161 caacgacgag atcggcaagc tgtccgacac attgaactac cttgccgagg aggtcgagaa
     5221 aagggacagg atgaagaatg aattcatatc ctccatctcc cacgaactga ggacgcccct
     5281 cacatcgatc aagggctggg ccgtcacgct gaaggaagtt ggcagcgaag acagggaact
     5341 gcacgagaac ggactggaca taatcgaaaa cgagtgcgac aggctcacgg cgatggtgga
     5401 ggaactgctg gatttctcaa ggctggtttc gggcaagctt atgataaaaa gggagaacgt
     5461 gcagctggag gaattcgtcg acacggtcgg caggcagctt gcgcctaggg ctgcaaggga
     5521 cggtctccac ttcagcgtcg agatggaagg agaactgcct gagatcgtta ccgatggcaa
     5581 caggctgaag caggtcatca tcaacgtgct cgacaacgca ttcaggttca ccccggccgg
     5641 cgggagcgtc ctgttcaaga ccgtggccag cggcggcgag gtgatcttta ccgtgagcga
     5701 tacgggctgc gggataccgg aggatgaact gccgtatata aaggagaaat tcttcaaagg
     5761 gaggaacagc aagcttggca gcggtctggg cctgtccatc tgcgatgaga taatcaagat
     5821 gatgggcggc agcatcaaca tatacagcag ggaaggcaaa ggcaccaagg tagtgatacg
     5881 gataccctgt atcatggaag gaggcgcggc atgatgaaaa aacagcattg cggaagactg
     5941 cgccgcatct tcatgatgct gttggccctg gcgctgacgc tgtccgcatg tacgcaggcg
     6001 gtgccggaga gcacggacag gataatcagc ccggtcaaca tggcagtgcc tgccgcgggc
     6061 acatgggtgc tggaaagacg gcttacagat gaaataactg atatatcgga tgcggatgac
     6121 agcctgaccg gggagaaggt cggattcact cagaacacca tgttttacgc cggccaggtc
     6181 ttcactgata taagctacaa gataaaacgc gtcaatgtgt acgagtactt ccttcacaaa
     6241 aacagggata tcctgaaaaa actgaattat gaaaacaacg agatacttgt tgtcaacgta
     6301 tattcgaagg acagcttcct gtttgaattc ataatcgatt cgaatgatga aaaaatagct
     6361 gttatcgacg accgtttata tgtgatgaga aaggtatccg atgatgtcag cggcgaacag
     6421 gagataaccg cacatgatac gccggaatca gacatggaag atgccattgc gcagaaacag
     6481 cctctgcgct caggactgct gatgggtgtg cgctatcctg tccagacttc tgacgggctc
     6541 ggcgattaca aatacggaac atactggata tcatgcatca acggcgcgat aaatcctgta
     6601 ctgtatgctg acgctatata cctgccgagg atggacggct tctggaaact gatggtggaa
     6661 aagaggcccg gtccatcagg ccttgtagac acactgcgcg cctttaaggt cacgaacact
     6721 tccaataaag ccgtcgacag gccttctgcg gccagccctg aaaatgtatc ggaaaggatg
     6781 gagaccaggg tacgccgggc gatcatttat gtgggcaatg attacgcctg cgtcgaaaac
     6841 accatttacg gcacggacag tgacaatgca ggagcgattt atgaaaaaaa cctcaggacg
     6901 ctgcccgttg ataacctgat gaacgtcgac ggcataaaga tatccgacat ggcgggggaa
     6961 aacgggacca tggcgatgga gaacgccata actgaagtgc tggtcaattc aggatatgag
     7021 ggcatagtta tccttgacga tgatgagcag cagaagaatt tcgcactcta caggaaaaca
     7081 ggccattggt tcttcaaagg caggatcgac ccggaccgcc agggcaatct gccttacatg
     7141 gatttcaacc ttaatctaat cccgccgtcg aacatggtag cctatgatgt gctccatgtg
     7201 ccgtggaagg aaatgaagga caagcttccc aatgcaatag acatatacac gtcgccaaac
     7261 agggacctgg ctgtcatcct gacaaggagc catatcctgc tttatgcgat cgaaggaagt
     7321 aaacttgcgg acgagccgct ggcgaagatc cagcttgagg aaggcagcgc cgtgataatg
     7381 gctgagtggt gcatgggcga gtacgtatcc aggtgggagc agtctttcat caggaacaac
     7441 gatacgcgcg aagtttttga cgggaccggt gacagtgaag ctgtacagga ttgagcttga
     7501 atagatcgat cccgggccgt catgatatcg aactattgga tatagaatta tctgagacat
     7561 ctgactgaca ggaaatcgat atcaaacagg atctgatctg ccttattttc taaagcggac
     7621 cgtaatgaaa catgtcctgg gtcaaggttc gggtatatcc gggtcacgac tggaattcag
     7681 cctgtccttt tttgttttcc atattgattt taagcgaaat atgtgctaat attacacaaa
     7741 tgaaacacaa atgtgcgcct accgtggaca ggtgccagat accttgcgtg gcgggaggag
     7801 aatatggaga gcaaatcgat gtacctgctg gtggacagtg cgatcctgcc ggaagttttt
     7861 gcaaaggtca tagaggccaa gaaactcctg agttccggaa aggcaaagac ggtgaacgat
     7921 gcggtttctg cagcaggcct gagccggagc gctttttata agtacaggga ccacgtgttc
     7981 ccgttctatg agacttcaag gggcagggtg atcacactgt actttgccgt ggaggatttt
     8041 tccgggattc tctcaaggat catcaacaag atcgccgagg cgagggcgaa tatcatcact
     8101 ataaaccaga acgtgccgat aaacggcctt gcggatgtca ccatatcgat ttccaccgcc
     8161 ggaatgacca tggatcttga taagctcatg gaaggcattg ccgagataga aggcgtccgg
     8221 aaacagcaga tacttgcgag ggactgactt aaggaggctg atatcatgga tatagcgata
     8281 cttggatacg gagtggtcgg ctcgggcgta gccgaagtaa taaggaaaaa cggccaaagc
     8341 attgcagaca gggccgggga aaagatcaag gtaaggaaga tactcgacat cagggatttc
     8401 ccggacagcc ccgaccgtga cctcatgacc aaagatcccg aagaagtatt cggcgacccc
     8461 gatattaaag tcgtcgtaga gactataggc ggcaccggcg ttgcccggga atatacgaag
     8521 agggcgttca atgcagggaa acacgttgtg acatcgaaca aggagcttgt cgccgtgcat
     8581 ggtcctgaac tgctgaaact tgctgccgac aacggcgtaa gttatctctt tgaggccagt
     8641 gtcggcggag gcatacccat aatcaggccc ctgagccagt gccttgcggc gaatatcata
     8701 aatggcatca cgggaatact gaacggcacc accaactata tactcacgaa gatgcgcagg
     8761 gaaaactgca gcttcgatga agccctgaaa aatgctcagc tgaacggtta tgccgaagcg
     8821 gatcccaccg ccgacataga gggacatgac acctgcagga aaatcgcaat actttcgtcc
     8881 attgcctaca atgagtatgt ggactgcagc aggatccata ccgaaggcat atcccgcatc
     8941 acgcttaccg atatgctgta tgctgatgcg atggattccg taataaaact gatagcggtc
     9001 agcaggaggg aaggggacag ggtgttcgcc cgtgtatcgc ccgcagtcgt cagcagggag
     9061 catccccttg ccggcgtcga ggatgtattc aacgctatcg tggtaagcgg cgatgccatc
     9121 ggagacgcca tgttctacgg aagaggggcg ggcaagctgc ctactgcgag cgctgtagtg
     9181 gccgacgtta tcgatatagt gaagcataac gagagcacaa gatccgtatg gcagcggaaa
     9241 gaaacggaca acatggtgcc cttcggcgaa agcgtgacca gatgcctggt cagggtcgca
     9301 gtaaagaaca ggaacgaagc tgccgctgat atcgggaaga tattcggcgg ggttgaatat
     9361 gtcagcgcag acataaaagg catcgatgac gaacttgcat tcacgaccgg gaaaatgaca
     9421 gaatcgagcc tttcggaaag ggtcggcgaa ctgaagtcga tgaggtctgt aaaagaggtc
     9481 gtgaatgtca taaggatact ggagcagtga gctgcaggaa ggcggaaaag ccgcagcggt
     9541 aatggaacag aaatggtccg gcaattggac atgtccggaa aaataaacat tgaaatgtcg
     9601 aacacattga tataaactag tagtataaaa agacaactta atacatgtac aggtatccgg
     9661 ccgtcaggcc ggtgcagagc gtaagctcga acagggaagt tgggtttgaa tcccacgcgg
     9721 tcccgccgct gtattagaag agttctgccg cattaccact gggcaaccgg gaagggcggc
     9781 agagcgatga cgcttaagcc agaagacctg cctgtacgtc aacaccgtta actctacgag
     9841 agataggggg tgtgatggat gtcgaaagaa catcttattg tgtatatcag agcctcctga
     9901 ccttttgtcg ggaggctttt atcacgtatg gacgcgcctg tgcggaccag gccggacgcg
     9961 gcaggctacc ggatgccgtt atcagcgctg ccgtctttgt gaacacttct gtttgcgtaa
    10021 aagcgctccc gttgttttca ttgcaggaaa cgatcatgtc aggaaaggaa aaagcgtatg
    10081 aaaggtttga tacatatcta caccggcgac ggcaagggca aaacgactgc tgccataggt
    10141 cttggagtca gggcctgcgg ccgcgggatg aaggtgatga tggcgcagtt cctgaaaagc
    10201 gctccgacag gcgaggtgta cagcctgaaa tcactcgagc cggacttcgt actctgcagg
    10261 ggcacaggat ccaaaaagtt cacctggcag atggacgaga gcgaaaaagt ccaggctgcc
    10321 gcggagcagc aggcgatttt caactatgcg gtcaatgccg cccaaaacgg agaatgcgac
    10381 cttctgatcc ttgatgaggt attgggagcg atcacgaccg gtatgatcga aaaggaacaa
    10441 ctggtcagct ttatccgaaa caagccggac aggctggagc ttgtactgac cggcaggaat
    10501 gccgacccgg aactgatcgg gctggccgac tacgtgtcgg agatcatggc tgtgaagcat
    10561 ccggctgaaa aaggcataca tgcaagaaaa ggcatcgagt tttagcagaa ggacattagt
    10621 agaaggacaa aagaagattt tgcagggagg atgaaaaatg gtttttatga agagttttgc
    10681 gaagaacgct gatataaatc caaggcagga aagaaaaacg gctgattgcg gaaaaaaaat
    10741 aaatcttatc ggcaggtttg ccgtcaggag gggtttggtt ctactggtgg ctgtaataat
    10801 gctgcttgca gtccttgctg gctgctccgg acaagaccgg gcgggcagca taagcgataa
    10861 tgccggtctg cagtccggcg ccggatctga agatgccgta gccggaaacg gaactgctgg
    10921 cgcatccgga gatggatcgg ggagtatttc gggaggatca tttgatactt ccggcggttc
    10981 tgagaccgga gccggcggag acgggaccgc tgccgggaca aatgcatcct cgggattccc
    11041 gctcactgtg accgatgcca acggggatgt aatgatactt gagaatccgc cgaaaaggat
    11101 catatcgctg accctcgggt ccgacgagat acttctggac cttgtcgata agagcaggat
    11161 cgtttcgctg acaaggtatg cggatgacga aggtatatcg aatattgccg ccgaagccag
    11221 gggcgtcggg gtcaggacta cgatggatca aatcgagaac atcattgccc tcgaacctga
    11281 ccttgttatt actgacacct gggcggatgt gaattatata aaacagctga gggattcagg
    11341 gatcaccgtc tatgccttca ggacaccgaa caatatagac gaacagaagg ccgtcatcgc
    11401 ggaactggcg catgtggtcg gcgcagatga gaaggggcag gagctgataa actggatgga
    11461 tgggatactg aaggccgtgg aagagaggct ctcagtcctc gagcccgggc agaagctcac
    11521 ggtgatggat tacggcgaac ttggcagcag cggaaagggt accaactttg acgatattgt
    11581 gacacgggcc gggctggtta acgtaatatc gcgggcaggc atagagggct ggcctgtgat
    11641 ctccaaggaa aagataatcg agttcgatcc ggatatcata atccttcctt cctggtatta
    11701 tgaccccggt aatacattcg agagcatgag ggattcactg gtaaatgatc cgagcctgca
    11761 gaccgtcagc gccatcaaaa acaacaggat catttccgtc ccgaatcccc acataagcgc
    11821 gatatcgcat tatgtcgtgc tggcggtgga ggacgtggca aaagcggcct atcctgaact
    11881 gttcgaataa gaaaattgca atggagaagt cataaagatg ctgaaaacgg gcaatctcac
    11941 tgtatataaa aaaatgagcg tgatcctgcc ggtattggtg gcgctgctgg cgttcatgat
    12001 aatacttgcg gtagccctgg gcgcggtata cgtgcccttt ggcaggtcat tcaggataat
    12061 actcgacaat atcggcctga tagacgatcc gacgctgccc gcggagcagg tctccataat
    12121 ctacatggta aggctcccgc gcgtcatcgt cgcatgtgtc gcaggtgcgg cgctgtcggc
    12181 atgcggtgcg gtcatgcagg ggcttttcag gaaccccatg gccgatcccg gtgtgcttgg
    12241 agtgtccagc ggcgcaggac ttggcgctat catctcgata acgacagggc tcgctgccag
    12301 gagcatatac acgatgccga tgttcgcggc ggttggggcg cttggcgccg ttgcagtcat
    12361 ttacctgctg tcattgcgca ggggcaggat cgcgccgctg accctgatat tgtcgggaat
    12421 agctgtcagt actttcatag gcgcggtaac gcagttcatc cttatcaaaa gcaacacata
    12481 cgaagtgcgc aggtacgttt tctggaccat gggcggcctc agcggcatga tgtgggagca
    12541 ggtaaggctc gtcactttcc ccgtgcttgt gctgttggcc gcgctgatga tgttttcacg
    12601 cgacctgaac ctgctgcagc ttggcgatga ggaggcccag tcccttgggc ttgagccttc
    12661 ccgcacaagg aagctgctgc ttctcataac atcgctcata actgctgtga cgatatccgt
    12721 atgcggccct gtaagcttcg taggcctgat agttccgcac ataatgaggc tgatagccgg
    12781 tccggaccat cgcctgctca tacccgccag cgccctcggg ggggccatat tcctcaccgg
    12841 atgcgacata cttggccgta tgcctgcaaa cggcgagata cacgtaggta taataacggc
    12901 gatgctcggc gcgccttatt tcctgtacct gctgatcaga acgaggaagg gcggtgctgc
    12961 gttttgaacg gcgaaagatc cggatatgac ggcggcaggt ccggcagcag cggccaggat
    13021 tccaaactgc tcagggttga taatctgaat taccatacgg acggcgtcca tatccttaaa
    13081 gatatttcgc ttgaagctga aaaaggggaa ttcatcggac ttatagggcc gaacggggcc
    13141 ggaaagacca cgcttttgaa atgcatcaac ggcatcaaca ggggcaccgg gctcgtagag
    13201 cttggcggca gggacatcag gacgatgagc agcaggcaga tcgcttcgat ggtcgcaatg
    13261 ctgcaccagg atactgccgt tgcgttcccg tttacggtgc tggatgtcgt tttgacggga
    13321 agataccaca agctcggccg attcaggccg gaaacgcctg aggactatgc ggtggcgcgc
    13381 aggtgcatgg agtacaccga cacgctgaga ctggaaagca gggcaataaa caccctttcc
    13441 ggaggagaga ggcagagggc gcttttcgcc agggtgcttg cacaggagac cgacctgata
    13501 ctccttgacg aaccgtctgc cagcctcgat atagcctatg aagaacagct tttcaaaaat
    13561 gcgcgcgaac tgggcgaagg cgggaaagca gtcattgcgg cggtacatga tctgaagatc
    13621 gccgttcgct attgcacaag gctcataatg atgaaagacg gcaggataat agccgacggc
    13681 agccccgaat ccgtaattac ccgggaaaac ctgagacagt cgtatggcgt tgacgccatc
    13741 gtgtacagga acagggtgac cggcctggtg gacttccata tatccgggga aagccgcagc
    13801 aggaaagata tcagcgtaca tgtcataggc gggggaggtt ccgcatccgg cgtcatacgc
    13861 cagcttttcg aactcggctg caatataacg gccggcgtgt tttcccacgg tgacagcgac
    13921 ctggcgtgtg ccggcatatt cggggtcagg acggtgactt gcaggccatt cagcgagata
    13981 gacgatgaca gtttcaggga gaacgtggaa cttgtgaagg cctcagacat gaccatattg
    14041 tgcgacatgc cattcggctc ccagaatata aggaatcttg aagcagccag gcatgcttcg
    14101 gaactggtca tcatagagga cagcgacccc cagagccgtg actacacggg aggcagggcg
    14161 ctggaaatat acaatgaact taaaaagaga gcagttgtgg tggaatccgc aaggctgcat
    14221 gaagt
//
LOCUS       DAOSCC010000034        13809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3000_length_13809_cov_4.855315, whole genome shotgun sequence.
ACCESSION   DAOSCC010000034 DAOSCC010000000
VERSION     DAOSCC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3000_length_13809_cov_4.855315"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /gene="thrS"
                     /locus_tag="PLG72_03215"
     CDS             complement(<1..1455)
                     /gene="thrS"
                     /locus_tag="PLG72_03215"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ79843.1"
                     /translation="MINVTLKDGSTKQYPDGITVKEVAGSISAGLARVALAAEIDGEV
                     KDLSTPLDKDCTLNLLTFDSEGGKHAFRHTASHVLAQAVKRLFPSVKLAIGPAIENGF
                     YYDFDADRPFTQEDLEKIEAEMEKVVKEDLQLERFTLPREEAIRFMEEKNEPYKVELI
                     RDLPENAVISFYRQGDFIDLCAGPHIPSTGRIKAFKLTHVAGAYWRGDEKNKMLQRIY
                     GTAFPKKSELDEYLAAMEEARKRDHNKLGRELGYFTTVDYIGQGLPILMPKGAKVIQI
                     LQRFVEDEEERRGYMLTKTPFMAKRELYKISGHWDHYRDGMFILGDPAKFEDPNEEIF
                     ALRPMTCPFQFQVYLSRLRSYRDLPLRYNETSTLFRNEASGEMHGLIRVRQFTISEGH
                     IACLPEQLEEEFAGCLDLALFMLRTVGLESDVSYRFSKWDPNNKEKYIGTEEQWEEVQ
                     GRMRQILDDLKLDYTEAEGEAAFYGPKADFMVRDV"
     gene            complement(1971..2864)
                     /locus_tag="PLG72_03220"
     CDS             complement(1971..2864)
                     /locus_tag="PLG72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79844.1"
                     /translation="MITNILTPFGIAQPQLAEGVQQLDAAGNPLFGPAADYGLLTIIS
                     TMSWGLGYFGMPQVLLRFMAINKSTELKKSRRIAIIWCVISLFAAVAIGLIGRSLYPA
                     ELLTKSQSENIFIIMSAGLLPAFLAGIVMSGILAATMSSSDSYLLIASSALSRDIFKG
                     VFKKDATDNQVMIVSRITLLVVSLFGIIIALDEESVIFDVVSFAWAGFGATFGPIILF
                     SLFWKRTTRAGAIAGMVTGGSMVFIWNKLISRLGGVFAIYELFPAFVLSCIVIVIVSL
                     LSKKPDQEIQDEFVAAKTFEC"
     gene            2886..3200
                     /locus_tag="PLG72_03225"
     CDS             2886..3200
                     /locus_tag="PLG72_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79845.1"
                     /translation="MLIIAVITVTAILSAIFAAASGCRSSGTIRTKLLYTLGLCLIDT
                     SAEVAFFMFSITVAITFGIMIFSFTRLSSLWVMTPDILITLSSLILIRCHRLKIHSLA
                     SI"
     gene            3349..3819
                     /locus_tag="PLG72_03230"
     CDS             3349..3819
                     /locus_tag="PLG72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79846.1"
                     /translation="MQITGGVNIYITYLTAKEAGFDYLRSFLAMSPYDLVQRPFNYAI
                     VDEADSILIDEARIPLVIAGKIEKTHDIDPVRMAEVIEGLVPGKDYDLDEYLVLSGAK
                     KAKGVKKGGAADVRLVFPGNCSIRCYRLELYFKHKSHDEAKKYCAYAAIWEILK"
     gene            3816..3965
                     /locus_tag="PLG72_03235"
     CDS             3816..3965
                     /locus_tag="PLG72_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79847.1"
                     /translation="MRYKRKTYFRKLRHKKVRKALLYGLVMPSALILLSYLVASFVIL
                     PAMSG"
     gene            complement(4040..5101)
                     /locus_tag="PLG72_03240"
     CDS             complement(4040..5101)
                     /locus_tag="PLG72_03240"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ79848.1"
                     /translation="MKKLLFAIMITILSISLAACSLPGKDAATSGNDAGNQQDDGETG
                     FVETGDDEQNSGDAGEDRDIPGDDGGGTDEDTKMIKVALYFPTIDNSALKKVEKEIPV
                     VNKAILKACMLALIEGPGDPALRKPIPPGTEILGISIKDGTAIVDLSKEFLNSSGLDE
                     VTSRLSIVNTLTEIEGVDRVRLRIEGEDMAGPSGKPLGDMEPARLDEEGRPLPGKTMT
                     VVLYFSDNEAMYLVGEPRDIEIPAGVSPEEAVLAELMAGPRNDDLWDAIPDGTKLLSV
                     DTKDGVCTVNFSREYIENSPGGTAAERMAIFSVVNTLTELEGIDKVQFLVEGRKEKIY
                     THAIFDEPFSRDESIISKQ"
     gene            complement(5242..6081)
                     /locus_tag="PLG72_03245"
     CDS             complement(5242..6081)
                     /locus_tag="PLG72_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ79849.1"
                     /translation="MKVRFLGKEMEIKTELLFSMVSALVLLGVLAGIFLFGGRGDIII
                     EEGQAIMQQTSGNGETNDAERDGALAGNGNRADDNYGSKAMEIANADQDPGGGPGIPG
                     NAGEDAKAGIGSNGKEDEIKVYVVGCVKNPGIVTLTRGQMVFDAVREAGGLTEDADAD
                     NINMVYSLNENVMLVIKSKKENKAVGEGAVVRSDSGPGAEVIGGGDDAANGRDDMRLV
                     NINTAGVDELDTLPGIGEAKARDIIAYREKHGRFGRIEDIMKVPGIKQNRFESIKDFI
                     TVD"
     gene            6224..7513
                     /locus_tag="PLG72_03250"
     CDS             6224..7513
                     /locus_tag="PLG72_03250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ79850.1"
                     /translation="MKKLLTLAMIVLLTASSIGIAYAQPPSLDARAYILIDAQTGDVL
                     CEKNSRETLYPASTTKIMTAILALELGDLDEVMTASQSAIDAIGYNGSNIGIIPGEKI
                     RMENLLQAMLISSANEAANIIAENISGSMEEFVELMNKRAKELGTVETHFANPSGIHD
                     TMHFTTAADLACISRYAMTLPKFREIVGTHSFTMPATNKHSKWPELTNSNKLMLNNTS
                     DLYEINGIKTGFTGPAGNCLISSARNEDGMELIAVVMGVKNENAAENVRKYSKELLDY
                     GFTDFERVELIQKGRVYRNIKVEDAADIYGLDLVTEDSLVRVLPKDLSQRNIQEISHI
                     NGEIHAPVNKGDRIGYVEFLKDGVSIGKVDLIAARDIEYKPNPVSLTALTQHPETILD
                     NIYFKIGICCAAFIIFLILVRLFVKALSRRSRSRRYY"
     gene            7607..9319
                     /locus_tag="PLG72_03255"
     CDS             7607..9319
                     /locus_tag="PLG72_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ79851.1"
                     /translation="MTSKPNREVRQIRDLKDMLVQSAELYGDNEAFYLKSNDGTYKGI
                     TYREFKREVDSFGTALVDLGLKGKSIAVIGENRYEWCLTYLAVANGVGVIVPLDRELP
                     KDEIENLLIRSHASAVVFSGKHEKDMRLISSAVPQVEYFINMDADEDDGRFLSMKKLV
                     RKGGDLLDEGDRTYIDAQIDPDAFGILIFTSGTTDLAKGVMLSHRNIVSNIMSVCSVL
                     YIDDTDSTLIMLPLHHTYACTTSFLLILYNGCRISFIEGLKHIAKNMKETNPSIIMAV
                     PLLLESMYKRIWDSINKQKSAYKVKVALAFSKLLLLLGIDARKKLFRQIHENMGGRLR
                     LIISGAAALDPAVSKGFRDFGIPLMQGYGLTECSPIVTVNQLERFRDDSIGLPLPCNE
                     VMIYKPNKEGVGELIVKGSNVMLGYFENSLATEKAFRDGWFHTGDLAKMTREGFFSIT
                     GRKKNVIVTKNGKNIYPEEVEAYLNKSPFIAESMVYGKEDEASEETFVYAMIVPAMDI
                     IKERLGKSEVTEDEVFRIIDAEVKNVNKKMPLYKRILRFTIRQEEFAKTSTRKIKRYM
                     EKIS"
     gene            complement(9500..10798)
                     /locus_tag="PLG72_03260"
     CDS             complement(9500..10798)
                     /locus_tag="PLG72_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79852.1"
                     /translation="MFFILVSFALTFIFSQILNKVNFSNRLEKNPHERKRAYVHRVFY
                     LNEYAKELRLNPDIAGRLFEEFDESNRKIRDVEGKYARKRTVLGFVSNYLTNNFISDV
                     IYISYLVFRAAVSRAISYSSVVVLWNTSGGLKRNLHDIAGVVPQIAENSLYIEKIRGF
                     LDYKQKITSKRNLPVPEKQGTLELRNVSFGYNENDGYILNNISLMIHPGERVALVGYN
                     GAGKTTLIKLIMRLYDPNEGEILYNGIDIRDYDVHEYRSRIGTIFQDFRIFAATVGEN
                     VVLDKDEETIEKESRPAVLKALEYSGFKERLDKLSNGLATELTTEFEQEGIDLSGGES
                     QKLAIARAFFKDAHMVILDEPSSALDPIAEYQLNKTMLQAAEGKSVVFISHRLSTTRH
                     ADRILMMENGRVIEEGNHDELIRLQGKYAAMWKAQAGRYI"
     gene            complement(10929..12206)
                     /locus_tag="PLG72_03265"
     CDS             complement(10929..12206)
                     /locus_tag="PLG72_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79853.1"
                     /translation="MEKQQAGKTRAIGNILYAVRLMWGMCRGIVIHTAVISAIDYFGW
                     VFYDLFFIRYLIGAIENQESFSYIMRFILISGLVFCMIFAYISYIDGSFLPRAGVTLY
                     RKLYGMLYKKAGSVELGCFEDSDFYNRYALAVDKADENLSMIVRNLFGIVFSGIAAVI
                     VFYTMYTIDSIAVLFVFFPIIGNFVFGYIYNKMLFKRDQELIPYKRRIEYVNRVMHLA
                     DFSKEMRLSNVYNLMKYKYKKALEGIFDTVEKYVLKINIPLWFRNYFTFTIIFEGVLL
                     YGAYRTIVSRTMDLSELAVLSTAMVSATWILIGFTDHILNSVKLGIFMENIRSFMEYE
                     PKLPEDQDGIIPGKEIESIEFRNVSFAYKDCGELPFDRIRGVGRFVPAQRDLSEPCVT
                     KGSAQNRKETAGYDVCQGKGTGPRMLRRSRVLQ"
     gene            complement(12468..>13809)
                     /locus_tag="PLG72_03270"
     CDS             complement(12468..>13809)
                     /locus_tag="PLG72_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ79854.1"
                     /translation="DTLPFVEDGILVDSAEFTGMGSAQARKEIVKKLNKQGRGEFKVN
                     YRLRDWLVSRQRYWGAPIPVIHCEHCGIVPVPEKDLPVELPYNVEFTPDGESPLAKCE
                     EFINTTCPKCGMPAKRDPDTLDTFVCSSWYFLRYPDNKNSEKAFDTEWINKMLPVDKY
                     VGGAEHAAMHLLYARFVTKALRDMGYLDFDEPFLSLVHQGTILGPDGNRMSKSKGNTI
                     SPDDYIQEYGSDVFRMYLGFGFAYTEGGPWSDDGIKAISRFVGRLERLIESASALDRT
                     IGGEYGSEEKELDFVRNTAIKGVTEDAERFQFNTSIARIMELVNALYKYESTGRKNNR
                     LYVDTVTDLLKLLAPFAPHLAEELWEKMGGPYSIFSQEWPKWDEKALVRDETEIVIQL
                     NGKIKEKVMVPSGLDKTQTEEAVMKNGKVAALLEGKTVVKVIAVPGRLVNIVVK"
BASE COUNT         3317 a         3663 c         3222 g         3607 t
ORIGIN      
        1 cacgtcgcgg accatgaagt cggccttcgg tccgtagaat gccgcctcgc cttcagcttc
       61 cgtataatcg agcttcagat cgtccagtat ctggcgcatg cgtccctgga cctcttccca
      121 ctgctcttcg gtgccgatgt acttttcctt gttgttcgga tcccattttg agaaacggta
      181 gctgacatcg gactcaaggc cgactgtcct gagcatgaag agggcaaggt ccaggcatcc
      241 tgcaaattcc tcttccaact gttccggcaa acaagctata tggccttctg aaatcgtgaa
      301 ctgcctgacg cgtatcagcc cgtgcatttc gccggaagct tcgttcctga acagcgttga
      361 agtctcattg tacctcagcg gcaggtcgcg gtagcttctc agcctgctca ggtagacctg
      421 gaactggaac gggcaggtca tcgggcgcag cgcgaatatc tcctcgttcg gatcctcaaa
      481 ttttgcagga tcacccaaaa tgaacatgcc gtccctgtag tggtcccagt ggcctgagat
      541 cttatacagt tcacgcttcg ccatgaacgg ggtcttggtg agcatgtaac cgcggcgttc
      601 ttcttcatcc tcgacgaacc tctgcagtat ctgtatcacc ttcgcccctt tcggcatcag
      661 tatcggcagg ccctgcccga tatagtccac ggtagtgaag tagcccagtt ctcttccgag
      721 cttgttgtgg tcgcgtttcc ttgcctcttc catggctgcg aggtattcat ccagttcgct
      781 ttttttcggg aacgcagtcc cgtatatcct ctgcagcatc ttgttctttt catccccgcg
      841 ccagtaagcc ccggcgacat gggtaagctt gaaagccttg atccttccgg tggacgggat
      901 atgtggccct gcgcacagat cgatgaaatc gccctgcctg taaaaagata tcaccgcatt
      961 ttcaggcaga tcgcgtatca gttcgacctt gtaaggctca ttcttttcct ccatgaaccg
     1021 tatcgcttct tcccttggca atgtgaatcg ttccagttgg aggtcctctt ttacgacctt
     1081 ctccatttcc gcttctatct tttccagatc ctcctgtgtg aatggcctgt ctgcgtcaaa
     1141 gtcatagtag aagccgttct cgatggccgg accaatggcc agtttcacgc tggggaacag
     1201 tctctttacc gcttgggcca atacatgcga cgccgtatgc ctgaatgcgt gtttgccgcc
     1261 ctcgctgtcg aacgtgagca gattgagggt gcagtcctta tccaggggtg tcgacaggtc
     1321 cttcacctca ccgtcgatct cggccgccag ggcgaccctc gccaggccgg cgctgatgct
     1381 cccggccact tcttttacag ttatcccatc cggatactgt ttggtgcttc cgtccttcaa
     1441 agtaacattt atcatacaga ctcctccttt tcacgatatt cgtcgaacca ggttcagcag
     1501 aaccagtacg gcattttctg tccgctgcgg cttttttaga tcgtaccggc tatgaccgta
     1561 ccgttatcca tgcttcattg ctcttccttc ttcagccgca tatactaaaa aagccctcgt
     1621 ccctgcattc tgcgcaaggg acgagagcgt gctcactcac gtggttccac cctatttggt
     1681 tctcattcga ccgtaacgcg gatcagcgtt cgcactcaca ggggcggtat tcggctgctt
     1741 tccttcaagg ccgcttccag ccggcggcgg cctctctctg ggaaggtctg tgacagctta
     1801 cttgatccct ttcatcgtgc gataactttt tatacgtttt aatttattat aatatatccg
     1861 acccgggccg atgtcaaccg gaatttactg aattttgaga taccggcatc tcagcctgac
     1921 cttcgggatg acatgcaggc tgaattttca tactgccatt taaaagttca ttaacattcg
     1981 aaagtctttg cagccacgaa ttcgtcctgt atctcctggt ccggtttctt cgacaaaagc
     2041 gaaacgatta ctataactat gcaggagagt acgaatgccg ggaacagttc atatatggcg
     2101 aacacaccgc caaggcgcga aatcagcttg ttccatatga ataccatgct gcctccggta
     2161 accatgcctg caattgcacc tgccctcgtc gtcctcttcc agaacagcga gaacagtatg
     2221 atgggcccga aggtcgctcc gaagcctgcc catgcaaatg ataccacatc gaagatgaca
     2281 ctttcctcgt ctagtgctat gatgatcccg aatagcgaaa caaccagcaa agttatacgc
     2341 gaaacgatca ttacctggtt gtcggtcgca tcctttttaa atactccttt aaaaatatca
     2401 cgcgataatg ccgacgaagc aatgagcaga tacgagtcag acgagctcat cgtagcggcc
     2461 aggatgcctg acattacgat acctgccagg aatgcgggga gcaacccggc ggacatgatg
     2521 atgaaaatgt tctcagactg gctctttgtg agcagttcag cgggatacag cgatcttccg
     2581 atcagtccga tcgcaaccgc tgcaaacagc gatataacgc accagataat tgctatgcgc
     2641 cttgacttct tcaactcggt ggacttgttt atcgccatga acctcaggag gacctggggc
     2701 atgccgaaat atccgaggcc ccatgacatt gtggatatga tcgtaagcag gccataatcc
     2761 gcagccggcc cgaacaaagg gttcccggcc gcatcaagct gctggacgcc ttccgccagc
     2821 tggggctggg ctataccgaa aggtgttaat atatttgtga tcataaacag ggaaagatgc
     2881 ctgagatgct gataattgca gtaattactg ttacagcgat actgtccgcc atatttgccg
     2941 cagcttcagg atgcagaagt tcggggacga tcaggacaaa gctgctgtat actttgggct
     3001 tgtgtcttat cgacacatca gccgaggtcg ctttcttcat gttttcaatt acagtcgcaa
     3061 tcacattcgg tatcatgata ttttcattca cccgactctc ttcgttatgg gtaatgactc
     3121 ccgacatact catcacatta agcagtctga tacttatcag gtgccatcgc ctgaaaatac
     3181 actcactggc ttcgatctga tcccggcatt cgatttgata ccaaaatgtc aggtcagaag
     3241 ctgcgccgac agaaatgcaa agcacgcttc tgtcttctcc gcatctttgc aaatcactga
     3301 tctgtatacc ggacaaaatc atcattgcag agattccgcc acttgtttat gcaaattaca
     3361 ggaggagtaa atatatacat aacatacctg acagccaaag aagccgggtt cgattacctg
     3421 aggagttttc ttgccatgag cccgtatgac ttggtgcagc gtcctttcaa ttatgcgatc
     3481 gtcgatgagg cggactcgat ccttatcgat gaggcaagga taccgctggt gatagccggt
     3541 aaaatcgaaa aaacgcatga catcgaccct gtccgcatgg cagaggtcat agaaggcctg
     3601 gttcccggaa aagattacga cctggatgaa tacctggtat tgtcaggtgc aaaaaaggca
     3661 aaaggcgtaa aaaagggagg agcagctgat gtccggctgg tatttccagg aaattgcagt
     3721 atcagatgtt ataggttaga attatatttc aaacacaaat ctcatgatga agcaaaaaaa
     3781 tactgcgcgt atgctgcaat ctgggagata ctgaaatgag gtacaaaaga aaaacatatt
     3841 tcagaaaact gcgccataaa aaagtgcgga aagcattgct gtatggactg gtaatgcctt
     3901 ccgcactcat cctgctcagt tatcttgtag cttccttcgt catactgccg gccatgtcgg
     3961 gctgaagcag gagtttgcat attgcttcag tgatattcgc ttcagcagca aattgaagcg
     4021 atttttttgc ttgccggtct tactgttttg aaatgatcga ttcatctctt gaaaacggct
     4081 cgtcaaatat ggcgtgagta tatatttttt ctttcctgcc ttctaccagg aactgtactt
     4141 tatcaatgcc ttcaagctcc gttaaagtat ttacgactga aaaaatggcc atcctttcag
     4201 cggcagtgcc tcccgggctg ttttcgatgt attccctcga gaagttcacg gtgcataccc
     4261 cgtcctttgt gtcgacagac aggagtttcg taccgtccgg tatagcgtcc cacaggtcat
     4321 cgttcctcgg acctgccatc agttcggcga ggactgcctc ttcaggtgaa acgcctgcgg
     4381 gtatttctat gtctcttggt tctccgacca ggtacattgc ttcgttatcg gaaaaataca
     4441 ataccacggt catcgttttt ccggggagag gccgtccttc ctcatcgagc cgggcaggct
     4501 ccatatcgcc gaggggcttg ccgctcggac cggccatatc ctctccttcg atcctgagac
     4561 gcaccctgtc cactccctcg atctccgtaa gggtattgac tatggaaagc ctggatgtga
     4621 cttcatcgag cccgctgctg ttgagaaact ccttcgacag gtctacgatc gccgttccgt
     4681 ctttgatcga tatgccaagg atctcggtgc ctggaggtat cggtttcctc agtgcaggat
     4741 cgccaggacc ttcgatcagc gccagcatgc atgccttcag tatagccttg ttgactacag
     4801 gtatttcttt ttcaactttt ttaagtgcac tgttgtcgat ggtcggaaaa taaagagcga
     4861 cctttatcat ttttgtgtct tcgtcagtac ccccgccgtc atccccagga atatcccggt
     4921 cctccccggc atcacctgag ttttgctcat catcgcctgt ctccacgaaa cccgtctcgc
     4981 cgtcatcctg ctggttcccg gcatcattgc ctgacgtcgc ggcatctttg ccgggcaaac
     5041 tgcaggctgc cagcgatata ctcaatattg tgatcattat tgcaaacagc agttttttca
     5101 tctgacccct ccgctcccgc catttgttca cgttactccc gcataacttc atttcattgc
     5161 cggctgcccg tatacccttt acagacggtt acgcgccatc gtatgtatag ttgagacgta
     5221 ccgccgcacg ggtatgttcc ttcagtccac tgtgataaaa tccttgatgc tctcgaacct
     5281 gttctgcttg atcccgggaa ctttcatgat atcttcgatc ctgccgaacc tgccgtgttt
     5341 ctccctgtaa gcgatgatat cccttgcttt cgcttcccct ataccaggaa gggtatcgag
     5401 ttcatccaca ccggcagtgt ttatgttgac cagcctcata tcgtcccggc cgttggccgc
     5461 gtcatcgccg cctcctatca cttcagcgcc ggggccgctg tcgctgcgta ccacagcgcc
     5521 ttcaccgacg gccttgttct ccttctttga ttttatgacc agcataacgt tttcattcaa
     5581 agaatacacc atgtttatgt tgtcggcgtc cgcatcctcg gtgagtccgc ccgcctccct
     5641 gacggcgtcg aataccatct gccctctcgt cagcgtaacg atgcctggat ttttcacgca
     5701 tcccaccaca tagaccttga tctcgtcctc tttaccattg gatccgatgc ccgctttcgc
     5761 gtcctctccg gcatttcccg ggatgcccgg ccctccgccc ggatcctggt cagcattcgc
     5821 tatttccata gctttgctcc cataattgtc atcggctctg ttcccattcc cggccaaagc
     5881 cccgtcacgt tcagcatcat tggtctcacc gtttcctgac gtctgctgca tgatcgcctg
     5941 tccttcttct atgattatgt ctcccctccc gccgaaaaga aagatccccg cgagcacgcc
     6001 cagtaaaacc agagcggaaa ccatcgaaaa aagaagttct gtttttatct ccatttcttt
     6061 ccctaaaaac cggactttca tagtatatta cctccatttt ttgtataatt ctccttccga
     6121 tgttattttc ctttattttc cgatacactt ttaatatttt ttaacttaca cagtaatatt
     6181 tttgctatac tttaaatgag aaaacatatc ccggagggta cagatgaaaa agctgctgac
     6241 attggcaatg attgtgctgc tgacagcttc gagcattggt atcgcatatg cgcagccgcc
     6301 ttcactggat gcaagggctt acatattgat agatgcacag accggcgacg ttctgtgtga
     6361 gaagaattcc cgcgaaacac tgtatcccgc cagtacgacc aagataatga ctgcaatact
     6421 tgcgctggaa ctcggagatc tcgacgaagt gatgacagcg agccagtccg ccatagatgc
     6481 catcggttat aacggaagca atatagggat aatacccggc gagaagatcc gcatggaaaa
     6541 cctgctccag gcgatgctta tcagttccgc aaacgaagcc gctaatataa tcgcagaaaa
     6601 cataagcggc agcatggagg agtttgtgga actgatgaac aagcgggcta aagaacttgg
     6661 gacagtcgag acccatttcg ccaatccgag cggtatccat gataccatgc atttcacaac
     6721 tgcggctgac ctggcatgca tttcaaggta tgcgatgaca ttgccgaaat tcagggaaat
     6781 agtgggcaca cacagcttta cgatgcctgc tacgaacaag cactcgaagt ggcctgagct
     6841 gacaaattcc aataagctca tgctcaacaa cacaagcgac ctgtatgaga tcaacggcat
     6901 caaaacaggg tttaccggac ccgccggcaa ttgcctgatt tcatccgcaa ggaacgaaga
     6961 tggcatggaa ctgatcgcag tcgtaatggg cgtcaaaaat gagaacgctg ccgaaaatgt
     7021 caggaaatac tcaaaagaac tgcttgatta cggtttcacg gatttcgaac gtgtcgaact
     7081 gatacagaaa ggccgggtat acaggaatat aaaggtggaa gatgcggcgg acatatacgg
     7141 cctcgacctg gtaacggagg actcgcttgt ccgtgtgctg cctaaagatc tttcacagag
     7201 gaatatccag gagatctcac atataaacgg agagatccac gccccggtca acaagggcga
     7261 caggatcggg tatgtcgaat tcctgaagga cggtgtttcc atcggtaaag tggacctgat
     7321 cgccgcaagg gatatcgaat ataaaccgaa ccctgtctca cttaccgctt tgacgcagca
     7381 tccggagacc atcctggaca atatttactt caaaatcggt atatgctgtg ccgccttcat
     7441 catatttctc atactcgtca ggctgtttgt aaaggcgctt tcacggagat cccgcagcag
     7501 aagatactat tgacaggcag gtatttacta gcaaactcaa cataatatat aatatatggg
     7561 cagagaaacg gcttattctc gccatttttt tgagaggtgt tatcaaatga catcgaaacc
     7621 aaaccgtgaa gtgcgccaga tacgtgacct caaggatatg ctggtgcaaa gcgccgagtt
     7681 gtacggcgac aatgaagctt tttacctcaa atcgaatgac ggtacctaca aaggaataac
     7741 atacagggaa ttcaaaagag aagtcgattc attcggcact gccctcgtgg atctagggct
     7801 gaaaggaaaa tccatagcgg tcatcggcga gaaccgttat gaatggtgcc tgacctacct
     7861 ggcagttgca aacggcgtcg gcgtcatcgt gccgcttgac agggagctgc caaaagatga
     7921 gatcgaaaac ctgctgatcc gctcacacgc cagcgcggtg gtcttttccg gaaagcacga
     7981 aaaagatatg cggctcattt catcagcagt tccgcaggtg gagtacttca taaacatgga
     8041 cgctgatgaa gacgacggca ggttcctctc catgaagaaa ctcgttcgta agggcgggga
     8101 cctgctggat gaaggcgatc gtacatatat cgacgcgcaa atcgatccgg acgcattcgg
     8161 catcctgatc ttcacttcag gcacaacgga cctggcaaaa ggcgtcatgc tttcgcacag
     8221 gaacatagtc tcgaacatca tgtccgtttg ctcggtgctg tatatcgacg ataccgattc
     8281 gaccctgatc atgctcccgc tccatcatac atatgcctgc accacaagtt tcctgctgat
     8341 actgtacaat ggctgcagga tatcctttat cgagggactc aaacatatcg caaagaacat
     8401 gaaggaaacg aatccgtcca ttatcatggc cgtaccgctt ttgctcgaat ccatgtacaa
     8461 gaggatatgg gattccataa acaagcagaa gtcagcatac aaggttaaag tggcgctggc
     8521 tttcagcaag ttgctgctgc ttcttggcat agacgcaagg aagaagttgt tcaggcaaat
     8581 acatgaaaac atgggcggca ggctcaggct gatcatttca ggtgctgcgg ccctcgatcc
     8641 tgccgtatcg aagggtttcc gcgatttcgg aattcccctg atgcaaggat acgggcttac
     8701 ggaatgttcc cccatcgtta cggtcaacca gttggagagg ttcagggacg attccatcgg
     8761 gcttcctctt ccctgcaatg aagttatgat ctacaagccg aacaaggagg gtgtcggaga
     8821 gcttatcgta aagggcagca acgtgatgct cggatatttc gaaaacagcc tggctactga
     8881 aaaagcgttc agggacggct ggttccacac cggcgacctg gcgaagatga ccagggaagg
     8941 tttcttcagc ataaccggca ggaagaagaa cgtcatagtc acaaaaaacg gcaagaacat
     9001 atatcccgaa gaggtcgagg cttacctcaa caaaagcccg ttcatagctg aatcgatggt
     9061 ctacggcaag gaagacgaag cttccgaaga gactttcgta tatgccatga tcgtcccggc
     9121 catggatatc ataaaagaaa ggcttggcaa gtccgaggta acggaggacg aagtcttcag
     9181 gatcatagac gccgaagtca agaatgtcaa taagaagatg ccgctgtata agcgcatact
     9241 caggttcacg atccgccagg aagagttcgc caagacttcg accaggaaaa taaagcgcta
     9301 tatggagaaa atcagttgaa agaggtacgg tgacactcct ctgcaagggg tattcctctg
     9361 tggccgagga atgtcacacg aatgaaaagt acggtgacac tcctctgcaa ggggtattcc
     9421 ttgagggatg aggagtgtcc cttttttatt gcgccttctg gcagcagcca tgtcctggta
     9481 cagcctatat ttcagatcat taaatatacc tgcctgcctg ggccttccac attgcggcat
     9541 atttgccctg cagcctgatc agttcgtcgt ggtttccctc ttcgatgact ctgccgttct
     9601 ccatcattaa tatgcggtct gcgtgacgcg tcgttgaaag cctgtgggaa atgaacacga
     9661 cggatttgcc ttcggccgcc tgcagcatgg ttttgtttaa ctggtactcg gctatcgggt
     9721 caagagcgct ggatggttcg tcaaggatca ccatatgggc atccttgaaa aaagcacggg
     9781 ctatggcaag cttctggctc tccccgcccg acagatcgat accttcctgt tcaaactcgg
     9841 tcgtcagctc tgttgcaaga ccgttggaca gcttgtccag gcgttcctta aaaccgctgt
     9901 actccagagc tttgagcacg gccggcctgc tttccttttc aatggtttcc tcatccttgt
     9961 cgaggacaac attttctccg acagttgccg caaagatcct gaagtcctga aaaatcgtcc
    10021 cgatacggct tctgtactca tgtacgtcat aatccctgat atcgatgccg ttatacagga
    10081 tctctccctc attgggatcg tagagtctca ttatgagctt gatcagcgtt gtcttgcctg
    10141 ccccgttgta tccgaccagg gccactcttt caccggggtg gatcataagg ctgatattgt
    10201 tcaggatata accgtcattt tcattgtagc caaaagaaac attcctcagt tcaagcgtac
    10261 cctgtttttc agggacaggc agattccgct tgctggttat cttctgctta taatcgagga
    10321 acccgcggat tttctcaata taaagactgt tttccgcgat ctggggtact acgccggcta
    10381 tatcatggag atttctcttc agtccgccgg atgtgttcca cagcacgacg acgctgctgt
    10441 aggaaatggc tctgctcacg gcagcacgga atacaaggta ggaaatatat atgacatcac
    10501 ttatgaaatt gttggtcaga taattcgaga caaacccaag gacagtgcgt ttcctcgcat
    10561 acttcccttc cacgtcccgt atttttctgt tcgactcgtc aaattcctca aaaagcctgc
    10621 cggcaatgtc cgggttcagt cttaactcct tggcgtactc attgaggtaa aataccctgt
    10681 gaacgtaggc cctcttcctt tcatggggat ttttctcgag cctgttgctg aagttgacct
    10741 tgttgagtat ctgcgagaat atgaatgtca gtgcgaagga cacaagtata aagaacatcg
    10801 aaggccagtc cgtcgtcagg aagaaaacgc ccctggttat cagcgccgtc atcccggaaa
    10861 aaatggattc cagtatctgg tgcgtccttg tgatggactt ctcagcttcc gataccgaaa
    10921 gcacgaattc attgtaatac ccgggatcgt cgtagcattc gaggtccagt tcctttacct
    10981 tggcatacat catatcccgc agtttctttt ctattttggg cagacccttt tgtgacacaa
    11041 ggttctgata gatcccgctg agcaggaacg aaacggccca cgccccgaat acgatcaaaa
    11101 ggaagttcac cacaatcctt gtatgcgaag ctgacattcc tgaactctat ggattctatt
    11161 tccttcccgg gaatgatccc gtcctggtcc tccggcagct ttggctcata ttccatgaaa
    11221 ctgcggatat tctccatgaa tatgcccagc ttgacgctgt tgagtatgtg gtccgtaaag
    11281 ccgatcagta tccaggtggc ggataccatg gcggtagaga gcacagcaag ttccgacagg
    11341 tccatggtcc tgctgacgat ggtacggtaa gccccgtaga gcagcactcc ttcaaagatg
    11401 atcgtaaagg taaaatagtt tctgaaccat agtgggatat ttattttaag gacgtacttc
    11461 tcgacagtgt cgaatatccc ttccagcgcc tttttgtact tatacttcat taggttataa
    11521 acattggaaa gccgcatttc ctttgaaaaa tccgcaaggt gcatgacccg gttcacgtat
    11581 tcgatccttc tcttgtacgg gatcagctcc tggtcgcgtt tgaagagcat tttgttgtat
    11641 atgtatccga aaacgaagtt gccgattatg gggaagaaaa caaaaagcac ggctatgctg
    11701 tctatcgtgt acatagtgta aaacactatc acggcagcaa ttccgctgaa cactatccca
    11761 aacaggttcc ggacgatcat ggacaggttt tcatcggcct tgtcgacagc cagcgcgtac
    11821 ctgttgtaaa agtcgctgtc ttcaaagcaa ccgagttcga cgctcccggc ttttttatac
    11881 agcatgccgt aaagcttgcg gtacagcgtc acaccggccc tcgggaggaa cgatccgtca
    11941 atgtatgaaa tataggcgaa aatcatgcaa aacaccagcc cggagatcaa aatgaacctc
    12001 attatgtatg aaaagctctc ctggttctct attgctccga tcagataacg aatgaaaaac
    12061 agatcataga atacccaccc aaaataatca atggcactta taacagccgt atgtatgact
    12121 attcccctgc acatgcccca catgagcctg acagcatata aaatattgcc gatggcacgg
    12181 gtcttgccgg cctgctgctt ttccattctt taggtacctc cccgtaatgc ggaacgcatg
    12241 ataatatatt acaccattac cgttatttcg aggtaaataa aaaataatta taggcaatat
    12301 gatgttacgt gtcatcaaca gaagtgatca aaatgacagc ggacagcctt tcttaagaca
    12361 tttgcagctt caaggcatat ctgtttcatg ggcatatttg catagatcct gtactgtgtt
    12421 tgccgtaagt ataaaatatg ggctgccttt caggcagccc tccttaatca cttcaccaca
    12481 atgttgacca gcctgccggg caccgcgatc accttgacga ccgtcttgcc ctccagcagc
    12541 gccgcgacct tcccgttctt catcacggct tcttcggtct gtgttttatc aaggcctgaa
    12601 ggtaccatga ccttttcctt gatcttgccg ttgagctgga taactatctc cgtttcgtcc
    12661 cttacgagcg ctttctcgtc ccacttgggc cattcctggc tgaagatcga atagggtccg
    12721 cccatcttct cccagagttc ctcggcaaga tgcggcgcga aaggagccag cagcttcaga
    12781 aggtccgtca cggtatcgac atagagccgg ttgttctttc tgcctgtact ctcatacttg
    12841 taaagcgcat ttacaagttc cattatcctc gctatcgatg tgttgaactg gaacctttcg
    12901 gcatcttcgg tgactccctt tatcgcggtg ttcctcacga aatccagttc cttttcctca
    12961 ctgccgtatt cgcctcctat agtcctgtca agtgcggaag ccgactcgat cagcctttcc
    13021 agccttccca cgaaccttga tatggccttg atgccgtcgt cgctccacgg cccgccttcc
    13081 gtatacgcaa agccgaagcc caggtacatc ctgaaaacat ccgaaccata ctcctgtata
    13141 tagtcatccg gcgatatggt attgcccttg gatttgctca tcctgttgcc gtccgggcca
    13201 agtatcgtgc cctggtggac cagcgaaagg aacggctcgt cgaaatccag gtatcccata
    13261 tcacgcaggg ccttcgttac gaaccttgca taaagcaggt gcatcgccgc gtgctctgcg
    13321 ccgccgacat acttgtccac aggcagcatc ttgttgatcc actcggtgtc gaacgccttt
    13381 tcggagttct tattgtcagg atagcgcagg aagtaccatg acgagcacac gaacgtgtcc
    13441 agcgtgtcgg gatccctttt agccggcatg ccgcatttcg ggcatgtggt gtttatgaac
    13501 tcttcgcatt ttgccagggg tgattctccg tccggagtaa attctacatt gtacggcagt
    13561 tctaccggca gatccttttc aggcacgggc acgatgccgc agtgttcgca gtggatgacc
    13621 ggtatcggcg ctccccagta gcgctgcctg gataccagcc agtccctgag cctgtaattt
    13681 accttgaatt cgcctctgcc ctgcttattg agctttttga cgatctcctt ccttgcctgt
    13741 gcggaaccca tgcctgtgaa ttccgcgctg tcgaccagta tgccgtcctc gacgaaaggc
    13801 agtgtgtcg
//
LOCUS       DAOSCC010000035        13502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3090_length_13502_cov_4.302893, whole genome shotgun sequence.
ACCESSION   DAOSCC010000035 DAOSCC010000000
VERSION     DAOSCC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_13502_cov_4.302893"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLG72_03275"
     CDS             complement(<1..1228)
                     /locus_tag="PLG72_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ79855.1"
                     /translation="MSFISRQLKKYLFFEEPEKLEKFILKPDGGTAGSKGAGTGSFEK
                     VREKKIAESDKPPEPGDEVKSDIDANITHMKDVYSIPVNSDIILREFDITYGCKSVRA
                     FIIFFDGMTDREVINNYILKPLMVNSVMPLKDTAENLEDYVARQLMPHCQVSIDKSYK
                     KAVEAVNFGGCALFVDGLAVCFNADVKGWDHRGVEKPNNEIIIKGPQEAFNEVLRTNS
                     AQIRKRLKDEDLIVEDVIVGKVSRTPCSIMYIKDVANEALVAEVRRRLEGIGVDYMLD
                     AGALEQLIEDSTVYPSPQMISTERPDYVADLLVSGRVAIIVDGSPNVLVVPVTAFDLM
                     HSPEDNYIRFPYVNLLRYIRYIAGFLALLLPGLYVAITNYHHEMIPTDLLLAIEASRE
                     KVPFPSLVEILLMEFSF"
     gene            1505..1780
                     /locus_tag="PLG72_03280"
     CDS             1505..1780
                     /locus_tag="PLG72_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79856.1"
                     /translation="MKNLKSISPAGLAIVSALAGIVLVLILDEDSLDAVGNVFVGIGG
                     ILLIAAAQNDYLDELEKAKLQNEIIEKQLEYFSRKRNSHKLSVKYVD"
     gene            complement(1964..2041)
                     /locus_tag="PLG72_03285"
     tRNA            complement(1964..2041)
                     /locus_tag="PLG72_03285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2004..2006),aa:Pro,seq:ggg)
     gene            complement(2158..2328)
                     /locus_tag="PLG72_03290"
     CDS             complement(2158..2328)
                     /locus_tag="PLG72_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79857.1"
                     /translation="MKNIIKLLVAVVIIAALVYVALGLLGFTIRVVLPIAILAFIAYI
                     IYVLVTGKRPNF"
     gene            complement(2469..3620)
                     /locus_tag="PLG72_03295"
     CDS             complement(2469..3620)
                     /locus_tag="PLG72_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ79858.1"
                     /translation="MIYLDNAATSFPKPECVCREMDRCMREYCANPGRGGYALSLRAG
                     MAVMRARDTVCRLFNIRDSMRLAFTKNATEALNIAIKGLASPGCHVITTAMEHNAVMR
                     PLRTLERDLGVEISVVKGNGFGEIDPFDIRREIKRNTALIVCTLSSNVNGIIMPIAET
                     GRIAREYGIPFLVDASQGAGSMPIDVEAWHIDMLAFPGHKGLMGPQGTGGLFVREGIR
                     LRALMEGGTGSNSEYVFQPEIMPDFLESGTLNTPGIVGLGAGADYVMKIGTSTVHEHK
                     SRLTRLLAEGLKNIPGVTLYSIVDKDRNSGIVAMNLSDMDPAKLSFLLDKDYGICTRA
                     GLHCSPAAHSSLGTLGRGAVRFSVGCFNTESDVIYAVEAVERTAYSASV"
     gene            complement(3714..4265)
                     /locus_tag="PLG72_03300"
     CDS             complement(3714..4265)
                     /locus_tag="PLG72_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ79859.1"
                     /translation="MKNRIQFLTRTALLLAVVIAFQLLGKAMPTYNNFIVGPVVNAAL
                     LIATAAAGLWSGIAISVIAPLVSAFTNKAPIAPLVLGFSPFIIIGNIIIVLAFWLFRK
                     KDRSPGNILGSVLGVVVGSVLKFGFLFGSITVFTRLVEMKPQQAATLTGLFSWPQLFT
                     AITGGVIAIAVLRLAGKALGLKE"
     gene            complement(4447..5025)
                     /locus_tag="PLG72_03305"
     CDS             complement(4447..5025)
                     /locus_tag="PLG72_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HOJ79860.1"
                     /translation="MQLKNVKIGFAFTGSFCTFSEVIAELEKLSAAGADITPIISYAV
                     DKFDTRFGSAEQWKIKIEAVTGRSVIRTITEAEPIGPKALFDILVVAPCTGNTLAKLA
                     NGITDTPVTMACKAHLRNGRPLLLAPSTNDGLGINAKNIGLLQSCKNIFFVPYRQDDP
                     QRKCNSLVARFDLIIPAIQAALEGRQLEPVLV"
     gene            complement(5029..5901)
                     /gene="dpsA"
                     /locus_tag="PLG72_03310"
     CDS             complement(5029..5901)
                     /gene="dpsA"
                     /locus_tag="PLG72_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HOJ79861.1"
                     /translation="MDSRKYAVIGGDLRNVKLAEAINSDGNHVSLFGFKNAGFSSSLT
                     ETEDLETAIAGSDIVIGPLPCTNDNEILNAPFHGSKIHISDVFSIMKENQLFIAGRIN
                     ERILQIAQAYNVRPIDILEREEMAVLNAIPTAEGAIQIAMEELPITLNGSRVLVLGYG
                     RLGKVLAKMLSGLGSEVYVAARKCSDIAWLKAYGIRPVYINELSKYLAEMDLIINTIP
                     SRILDYEKLELIGKECLVIDLASKPGGVDFDKAKLIGIKVIWALSLPGKVAPVTAANY
                     MKQTIYNILQELGV"
     gene            complement(6210..7463)
                     /locus_tag="PLG72_03315"
     CDS             complement(6210..7463)
                     /locus_tag="PLG72_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ79862.1"
                     /translation="MIRKFTLKNGVRVVCENIPYVRSVSTGIWVRTGSRNENPKNNGI
                     SHFVEHMLFKGTSTRTAAQIAECIDNIGGQINAFTGKECTCFYTKTLDEHIEIALDVL
                     GDMFFNSIFDKKDIALEKKVILEEISMYEDSPEELVHDLLSETVWADSSIGRPILGTK
                     SSLRNINRQMILDYINERYTQTNTVISVVGNYDEDRFEALLEKYFGDWEPKNGKDQEL
                     QRVVFKPGTGIKEKDTEQVHICIGYEGIKTGDDDIYPLLAISNILGGGMSSRLYQKIR
                     EEKGLVYSIYSYPTTYTDTGLFTIYAGMKPENLKVVASLIEEEIGSLRKNGIRQAELE
                     RTKEQMKGNYILGLESTSSRMNSIGKSELLLGYIYTPDEILDKISSITMDDIDRMIVR
                     IFGSDRKGLSVVGKINAGQCAAILG"
     gene            complement(7585..9693)
                     /locus_tag="PLG72_03320"
     CDS             complement(7585..9693)
                     /locus_tag="PLG72_03320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ79863.1"
                     /translation="MYRKFSMELAGRNLTIETGQLAQLANGSALVRYGDTVILSTATA
                     SPAPREGIDFFPLSVDYEEKLYAVGKIPGGFIKREGKPSEKAILTARVIDRPIRPLFP
                     KDLRNDVVVVNTVLSVDQDNSPEIAAMIGTSVALSISDIPFNGPIGGVVLGLVDGEII
                     INPNAAQREKSEMYVTLAGTSEKITMIEAGAKEVSEEIMMKAVKKGHEEIKKITEFIK
                     GIVAEVGKPKFEYQSAEVPKELYDAVYELTYEKLREAVLTDDKTVRDERVSAITEEVT
                     QQLAETFPESAAQIAEAIYKIERKIVRDYILKEGKRVDGRRLDEIRPMSAAVSLLPRA
                     HGSGLFQRGQTQVMTIVTLGSISEAQMLDGVDLEEEKRYMHHYNFPGFSVGEAKTSRG
                     PGRREIGHGALAERALEPVIPSEDEFPYAIRLVSEVLMSNGSTSQGSVCGSTLALMDA
                     GVPIKAPVAGISAGLVIDEENPERFVTFMDIQGIEDFFGDMDFKVAGTKKGITAIQMD
                     IKVDGLSYEIIEQALQLTRKGRWQILDEIILPVISEPRKSLSPYAPKMYRLDIDPDKI
                     REVIGLGGKVINRIIDETGVKIDIEDDGRVLVSTPDEEAGKRALAIIEAIARDVQPGQ
                     VFLGRVTRIMNFGAFVEFAPGKEGLVHISKLDNKRVSRVEDVCKVGDKMLVKVLDIDK
                     QGRINLSRKDALPENDTDKK"
     gene            complement(9912..10175)
                     /gene="rpsO"
                     /locus_tag="PLG72_03325"
     CDS             complement(9912..10175)
                     /gene="rpsO"
                     /locus_tag="PLG72_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ79864.1"
                     /translation="MQKEKKAEIIEKYKLHENDTGSPEVQIALLTERINHLNEHLKVH
                     KKDHHSRRGLLKMVGQRRGLLNYLMRKDIERYREIIDKLNLRK"
     gene            complement(10401..10559)
                     /locus_tag="PLG72_03330"
     CDS             complement(10401..10559)
                     /locus_tag="PLG72_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79865.1"
                     /translation="MKNIKGSGIVWIMLAVVLLTASVTSCTGGNLGIADTPGGQTADN
                     RQGRPRIM"
     gene            10834..11136
                     /locus_tag="PLG72_03335"
     CDS             10834..11136
                     /locus_tag="PLG72_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOJ79866.1"
                     /translation="MFGTNIMPEAYANIPSEVFAGSKAKEKAAAAQKISCHDFDMCKL
                     QYYDPQPIVGARLAHAYVPFQCLCCLYPPLLGLKQGTIFPELDRPYGADPAYSYDG"
     gene            11169..11414
                     /locus_tag="PLG72_03340"
     CDS             11169..11414
                     /locus_tag="PLG72_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOJ79867.1"
                     /translation="MHDAREKMLREIQALEFMEVELNLYLNTHPYDQQALMIFMNTAR
                     QQKMLRDNYERMYGPLTASSSNSFPWPWIESPWPWVA"
     gene            11458..11994
                     /locus_tag="PLG72_03345"
     CDS             11458..11994
                     /locus_tag="PLG72_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOJ79868.1"
                     /translation="MWVYEKKLQYPVKIKNCNPRLAKYIITQYGGPDGELAASLRYLS
                     QRYSMPTEVAKATLNDIGTEELAHLEIVGTIVYQLTKGVPPQILEKEGLGGYYADHDR
                     GIYPVNGDGVPFTAAYIGVKGDPIADLVEDLAAEQKARATYEGLINMTDDPDVIDPLR
                     FLREREVVHFQRFGETNR"
     gene            12250..>13502
                     /locus_tag="PLG72_03350"
     CDS             12250..>13502
                     /locus_tag="PLG72_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOJ79869.1"
                     /translation="MKNILINNVVEATLKYLQTMPKEKRKAIGQFFTSAETAQYMATM
                     FDKPKKKELSILDPGAGSGILSAAIIDRLQEDDTVKRIRLTCYETSEDILPILRTNLQ
                     YMKENSQKSMDYEIIEENYITSQAADFNQGMFASSNPIKYDWIIGNPPYMKIAKDSSE
                     ALAMPAVCYGAPNLYFLFAAMSLFNLDKQGEMVYIIPRSWTSGAYFKAFREYLLTEGT
                     LRQVHLFVSRDEVFENESVLQETMIIKVDKSNNRGTVKITSTRSNKDFNNINTIEVPY
                     NVIVYGVDKYVYLVTTKEELKVLQTLEKWKDTLPSIGLRMRTGLTVDFRSREFLRSEP
                     GEGVVPLFYAQHIQAGRVVFPVGKEGEYITTERSGLIQRNKNYLLIKRFTSKEEKRRL
                     QCGIYLASTQPDYNAISTDNKVNFI"
BASE COUNT         3353 a         3440 c         2923 g         3786 t
ORIGIN      
        1 caaaggaaaa ctccatcaga agtatttcca ccagtgaagg gaaagggacc ttttctctcg
       61 aagcctctat tgccagcagc aggtctgtgg gtatcatttc atggtggtaa ttagttattg
      121 cgacatacag cccgggcagc agcagtgcaa ggaatcctgc gatatagcgt atgtaacgca
      181 gcaggttgac gtacgggaac cttatatagt tatcctcagg agagtgcatc aggtcaaatg
      241 ccgtgacggg gaccaccagc acattcggcg aaccgtcgac gataatggcg accctgcctg
      301 ataccagcag atccgcaacg tagtctggcc tctccgtgga gatcatctgc ggcgacggat
      361 atacggtact gtcttctata agctgctcca gggctccggc gtccagcata tagtcgacgc
      421 cgattccctc cagtcttcgc ctgacctcgg ccacaagcgc ttcattggcc acatccttga
      481 tatacattat cgaacagggt gtcctgctta ctttgcccac gatcacatcc tcaacgatca
      541 gatcttcgtc tttcagcctt ttccttatct gtgcgctgtt tgtcctcagc acttcgttga
      601 atgcttcctg cggccctttg atgattattt cattattagg cttctcaaca cccctgtgat
      661 cccagccctt tacatccgca ttaaagcaaa cggcaagccc gtcgacgaag agtgcacaac
      721 cgccgaaatt cactgcttcg accgcctttt tatagctctt atctatgctt acctggcagt
      781 gaggcatcag ctggcgcgcg acataatctt caaggttttc ggcagtatcc ttgagaggca
      841 tcacggagtt taccatcaac ggcttcagta tgtaattgtt gatgacctcc ctgtcagtca
      901 tgccgtcaaa aaaaatgatg aatgccctga cagacttgca accgtaagtt atgtcgaatt
      961 ctctgagtat aatatcgctg ttcaccggga tgctgtagac atccttcatg tgcgtgatgt
     1021 tggcatcgat atcgcttttc acctcatcac cgggctccgg cggtttgtca gactcagcta
     1081 tctttttctc cctgaccttt tcaaagcttc ctgtacccgc gcctttactc cctgcagttc
     1141 ctccgtcagg tttcagtatg aacttttcca gcttttcagg ttcttcaaag aataagtact
     1201 tcttcagttg cctgctgata aacgacaaat cgaaagcctc ccgcatgtta agattttgtg
     1261 tgcgcagatc cgatatacga gccgtgtacc aatccgccag gcattacatt gcccggatct
     1321 gcctttgcgt atgccaactg aaatgttatg ccatattatg cctcattagt atcatcctga
     1381 tcggccgatt ttatgcagaa cgctgcgctc atggcaatta tggccgggct ttggcagtac
     1441 atacgcatta ctgcacatgt agactgcctt aaaagtatta tgtaatgaga agggaatgaa
     1501 acatatgaaa aacctgaaat ctatttctcc ggcaggtctg gctattgtat ccgctttggc
     1561 cggtatcgtt ctggtcctta tactggacga agacagcctg gatgcagtag ggaacgtatt
     1621 cgtcggaata ggcggcatac tgctcattgc agccgcacag aacgactatc ttgacgaact
     1681 ggaaaaagcg aaactgcaga atgaaataat tgagaagcag ctggagtatt tcagcagaaa
     1741 gagaaacagc cataagttgt cagttaagta tgtggattag tcaaccatca tgatttccgg
     1801 atggcagtca ccagttcggt cagctccgct ggcaaacgtc gaataatact tcatatttag
     1861 catgattagt aaattatcaa taaatcataa aataaattat tggttaatta tcgtgaaaaa
     1921 ataaaacagc ggatttcaga aatcctgaaa tccgctgttt cgctggtcgg agtgacctga
     1981 ctcgaacagg cgacctcttg caccccaagc aagcactcta accaaactga gctacacccc
     2041 ggcaacgcat aagaattata gcacaccagg agggtgttgt aaagcatcta tttgatcatt
     2101 cttctttttg cagaccggtt tatctgctca tttcgtcaga ttatccctgc tgttctgtca
     2161 aaagttcggc ctttttcccg tgaccagtac gtaaattatg tatgcgataa atgcaaggat
     2221 ggctatcggg agaacgaccc tgatcgtgaa accaagcagg ccaagcgcca catacaccag
     2281 tgcagctatg atgacgaccg caaccagcaa tttaataatg tttttcacaa ttatcacctc
     2341 tcataacatt ttatatttct gcaagctcac atattaccat ttatggaacc tgcatctgat
     2401 aataaatacg taaatgcatg gcaaatgtct tgcagaaata ctataaaaca tcatatcgca
     2461 gcccgtcatc aaaccgaggc actgtatgct gtcctttcga ctgcctccac agcatatatc
     2521 acatcacttt cggtattgaa gcatccgaca ctgaacctga ccgcccctcg gccaagagtc
     2581 ccgagtgaac tgtgggcggc aggcgaacaa tggaggcctg cccttgtaca tatcccataa
     2641 tctttgtcca aaagaaagct cagctttgca ggatccatat ccgacaggtt catcgcgact
     2701 attcctgagt tcctatcctt atccacaatg ctgtacagcg taactcccgg tatattcttc
     2761 aggccttccg ccaggagcct ggtcagccgg ctcttgtgtt catgaacggt gctggtcccg
     2821 attttcatca catagtcagc ccctgcccca agcccgacta tgccgggagt attcagtgta
     2881 ccgctttcca gaaagtcagg catgatttca ggctggaaca catattcgga gttgctcccg
     2941 gtacctcctt ccatcagcgc tcttagcctg atgccctccc tcacgaaaag gccgccggtg
     3001 ccctgcggcc ccataaggcc cttatgtccc ggaaatgcca gcatatctat atgccaggct
     3061 tccacatcta ttggcattga gcctgccccc tgcgaagcgt ccacgaggaa cgggatcccg
     3121 tattcacgcg cgatccggcc tgtttcagcg ataggcatta tgatcccgtt cacattggaa
     3181 gataaagtgc atactatcag ggctgtattc ctttttattt ctctgcgtat gtcgaaaggg
     3241 tcgatctctc cgaatccatt tccctttacc acggaaattt ctacgcccaa atcgcgttca
     3301 agcgtcctta atggcctcat tacagcattg tgttccattg ccgtggttat tacatggcat
     3361 ccgggtgaag ccagaccctt gatggcaatg ttcaacgctt ccgttgcatt tttggtaaat
     3421 gccagccgca tggaatccct gatgttgaac agcctgcaaa ctgtgtcccg ggcccgcatc
     3481 acagccatcc cggccctaag cgacagggca tatccccctc tgccgggatt ggcacagtat
     3541 tcccgcatgc acctgtccat ttcgcggcag acacattccg gcttagggaa agatgtggct
     3601 gcattgtcca gatatatcat caaaaaagcc cctcagcatt tagtccagta atatactatg
     3661 acattcgccg ggggctgtta actcatattt accggtattt ccgctcatcc atgtcattcc
     3721 ttgagcccga gagcttttcc tgccagcctg aggactgcga tcgcgataac accgcctgtt
     3781 atcgccgtaa acaactgcgg ccagctaaac aggccggtca gtgtggcagc ctgctgaggc
     3841 ttcatctcga ccagcctggt gaaaacagtt atcgagccaa acaggaagcc gaatttaagt
     3901 acagatccaa caacaacgcc cagtacgctc cccaggatat tgccgggtga cctgtctttt
     3961 tttctgaaca gccagaaggc cagtactatg atgatatttc cgattattat aaatggtgaa
     4021 aatccgagta ccaacggcgc tattggcgct ttgttggtga atgccgatac aagcggggca
     4081 attactgata ttgcaatgcc gctccacagc cctgctgcgg ctgttgcaat cagcagtgca
     4141 gcatttacga ccggtccgac aataaaattg ttgtatgtcg gcatcgcctt tccgagcagc
     4201 tggaatgcaa tcacgactgc aagcaataac gcggtgcggg ttaaaaactg tatcctgttc
     4261 ttcatattga ttctccccaa tatcctgctt tatatatatt tctattaaat tataccaccc
     4321 gggcagaaaa aataaaggta cagtaacgta cctcttccat ttatatattt acttcacctg
     4381 tttcctgcat ttctatatgc gaagagccag gtatgccggc agtaccggca ctgcaaacag
     4441 caggtctcaa accagtacag gctccagctg cctgccttca agagccgcct gtatcgcggg
     4501 tattatcaga tcgaatcgtg caaccagtga gttgcatttc ctttgcggat cgtcctgcct
     4561 gtatggcacg aagaatatat ttttacagct ttggagcagc ccgatgtttt tcgcattgat
     4621 cccaagcccg tcgtttgtgg acggtgccag caacaggggc ctgccgttcc gcaggtgtgc
     4681 cttgcatgcc atggtaaccg gagtgtcggt aatgccgttg gccagctttg ccagcgtgtt
     4741 tcccgtacag ggcgcgacca ccagtatgtc gaacaatgct ttgggaccga tcggttctgc
     4801 ttcggttatc gtccttataa cgctcctgcc cgttacagcc tctatcttta tcttccactg
     4861 ttcggcgctg ccgaacctgg tgtcgaattt gtctactgca tatgatatga tcggcgttat
     4921 atccgctccc gcagccgaca gtttctccaa ttcggctatc acttcggaaa aagtgcagaa
     4981 tgaaccggtg aatgcaaatc cgatcttcac gttcttcagc tgcatttttc acacccccag
     5041 ttcctgcagg atgttgtaaa tcgtctgctt catataattc gcagctgtca ccggggctac
     5101 cttgccgggc agcgacagcg cccaaatgac cttgatcccg atcagttttg ccttgtcgaa
     5161 gtccactcct cccggtttgg acgccaggtc tatgacaagg cattccttcc cgatcagttc
     5221 gagtttttca tagtcgagga tcctgctcgg gatagtattg atgatcagat ccatttcggc
     5281 aagatattta gatagctcgt ttatgtaaac cggccttatg ccataagctt tcagccacgc
     5341 tatatccgag cacttccttg ccgcgacata aacctccgaa ccaagtccgg acagcatttt
     5401 cgccaatacc ttgccaagtc ttccatagcc gagcaccagc accctgctgc cattcagcgt
     5461 tatgggcagt tcctccatcg cgatctggat agcgccttct gcagtcggta tcgcattgag
     5521 gacagccatc tcttcacgct caagtatgtc tatgggtctg acattatatg cctgagctat
     5581 ctgcaggatc ctttcgttga tccttcccgc gatgaacagc tggttttcct tcataatact
     5641 gaacacatca ctgatatgta tcttgctgcc gtgaaacggt gcattcagta tttcattatc
     5701 attagtacac ggcaatggac ctatgacgat atccgaaccg gcaatggccg tctccagatc
     5761 ttccgtttcc gtaaggctcg aactgaaacc tgcatttttg aagccgaaca ggctgacatg
     5821 gttcccgtcg gaattaatag cctcagccag cttgacattc ctcagatcac cgccgatcac
     5881 ggcatatttc ctggaatcca taactttacc tcccgatgct gacatggaca tcccctgttt
     5941 gtacaaaaac cgatctgtgc cggaatgtcc ttccaataac atgctaattc atattatgta
     6001 tatccccgga aacaggtgct tgttcagtaa gtgacgctgg caggcagaca cgggaaccgt
     6061 cgagacattt aaaacagggt gacggttctc atgtcagaga gccaggaacc gtcccctgta
     6121 ttaaaaagct ccgggactgt gctgatcgtt ccggagcatg actcttcaca ttgggatatg
     6181 gtctggcgag ggtcctgtac attggtatat catccgagaa tcgccgcgca ctggccggca
     6241 ttgatctttc cgacaactga taatcctttc ctgtcgcttc cgaatatacg gactatcatc
     6301 ctgtcgatgt catccatcgt tatcgaactg attttgtcaa gtatctcatc gggtgtgtat
     6361 atatatccga gcagcagttc ggatttgcct atgctgttca tcctgctgct tgtactttca
     6421 agaccaagta tatagtttcc tttcatctgc tctttcgttc tctccagctc agcctgtctt
     6481 atcccgttct tcctcaggct cccgatttct tcttcgatca ggcttgccac gactttcagg
     6541 ttttccggct tcataccggc atatatggta aaaaggccgg tatccgtgta tgtcgtagga
     6601 tatgaatata tcgaatagac aagccctttc tcctccctta tcttctggta cagccgggaa
     6661 ctcatgcctc ccccgagtat gttgctgatg gcgaggagcg ggtaaatatc atcatctccg
     6721 gttttaatac cttcataccc gatacagata tggacctgtt ccgtatcttt ttcctttatg
     6781 ccggttccgg gtttgaatac gacacgctga agctcctggt cttttccgtt tttgggttcc
     6841 cagtcgccga agtacttctc cagcagtgcc tcaaacctgt cttcatcata attgccaact
     6901 acggaaatga ccgtattggt ctgagtatac ctttcattta tgtaatcaag tatcatctgc
     6961 cggtttatat tcctcagcga gctttttgtc ccgaggatcg gtctgccgat ggaactgtcg
     7021 gcccagacag tttccgagag gaggtcatgt accagttctt cgggagaatc ctcatacatg
     7081 cttatttcct cgaggatgac ttttttctcc agtgctatat cctttttgtc gaagatggag
     7141 ttgaagaaca tgtcgcccag tacatccaac gctatctcta tatgttcatc gagcgtcttg
     7201 gtataaaagc acgtgcattc cttgcccgtg aaggcgttga tctgtccgcc tatattgtct
     7261 atgcattctg ctatctgtgc cgcagtccgg gtactcgtgc ccttgaaaag catatgctcc
     7321 acaaaatgcg atatgccgtt gttcttcgga ttttcattcc ttgatcccgt cctgacccag
     7381 atgcctgtcg agacagatct tacgtaaggt atattttcgc ataccaccct tacgccgttt
     7441 ttcaatgtaa actttctgat catattgcct ccctggatat tattatcgtc tacgcattat
     7501 tttatctata atgcacgaac caaataaaaa aggacggttt caacagttgc ctcagcctct
     7561 gcggaaccgt cctttttcac gatatcattt cttgtcggta tcgttctccg gcagcgcatc
     7621 tttcctcgac aggttgatgc ggccctgctt gtctatgtcc agtaccttga caagcatttt
     7681 gtctccgacc ttgcatacat cttccaccct ggacacccgc ttgttatcaa gcttggaaat
     7741 atggacaagc ccttccttgc cgggcgcgaa ttctacgaat gcgccgaaat tcattatcct
     7801 ggtcactctt ccaagaaaca cctggccggg ctgtacatcc ctggctattg cttctatgat
     7861 agccagcgcc ctcttaccgg cttcttcatc cggggtcgag accaataccc tgccgtcgtc
     7921 ttcgatatcg atcttcacgc cggtttcatc gattatcctg tttattacct tcccgcctag
     7981 tccgatgact tccctgatct tgtcgggatc tatatccagc ctgtacattt tgggcgcata
     8041 tggtgaaagc gacttcctgg gctcggatat cacaggcagg atgatctcgt cgagtatctg
     8101 ccagcgtccc ttcctggtaa gctgcagtgc ctgctcgatt atttcatatg aaagtccgtc
     8161 cactttgata tccatctgga tcgcggtgat gcctttcttc gtgcccgcca ccttgaaatc
     8221 catgtcgccg aagaaatctt cgattccctg tatatccatg aatgtgacga atctttccgg
     8281 attttcctcg tcgataacca gacctgcgga aatacctgca acgggcgctt tgatcggcac
     8341 accggcatcc atgagcgcaa gcgtgcttcc gcatacgctg ccctgcgagg tggaaccgtt
     8401 ggacatcaaa acttccgaaa ccagcctgat ggcatacggg aattcgtctt cgctaggtat
     8461 gaccggctcc agtgcccttt cagcaagcgc tccatgtccg atttctcttc ttccggggcc
     8521 tcttgatgtt ttcgcttctc ctacgctgaa gccggggaag ttgtaatggt gcatatacct
     8581 tttctcttct tcaagatcca caccgtcaag catctgggcc tcacttatgg aaccgagcgt
     8641 gacgatggtc atcacctgtg tctggcctct ctggaagaga cctgacccat gggccctcgg
     8701 aagcagagat acagccgccg acatgggcct tatctcgtcc agccttcttc cgtccaccct
     8761 cttgccttct ttgagtatat aatcccttac tattttcctc tcgatcttgt aaatggcttc
     8821 agcgatctgc gcagcacttt cagggaacgt ttcagccagt tgctgcgtta cttcctcggt
     8881 gattgccgac acccgttcat cccttacggt cttgtcatca gtcagcacgg cttcgcgcag
     8941 tttttcataa gtgagttcat atactgcatc atacagttcc ttcgggactt ctgccgactg
     9001 gtattcgaat ttgggctttc cgacttcggc aacgatgcct tttatgaatt cggtgatctt
     9061 tttgatctct tcatggcctt ttttgacggc cttcatcatt atttcctctg atacttcctt
     9121 cgcgccggct tcgatcatcg tgatcttttc gctcgtacct gccagcgtta cgtacatttc
     9181 gctcttttcc ctctgcgctg cgttggggtt tatgattatc tccccgtcga ccaatcccag
     9241 aacgactcct ccgatcgggc cgttgaacgg tatgtcggag atgcttagcg cgactgacgt
     9301 accgatcata gccgctatct cgggcgaatt atcctggtcg accgagagca ccgtattaac
     9361 aactacgaca tcgtttctca gatctttcgg gaataacggg cgtataggcc tgtcaatgac
     9421 cctggcggtc agtatagcct tttcagacgg ttttccttcc ctttttataa atcctccggg
     9481 tattttccct acggcataca gtttttcttc atagtctacg ctgagaggga agaaatcgat
     9541 cccttccctt ggagccggtg atgctgttgc cgtggacagg atgaccgtgt ccccgtatct
     9601 tacgagcgca gatccgttag caagctgcgc aagctggcct gtctcgatgg tgagattcct
     9661 gccggcaagt tccatactga attttctgta catataatgc ctcctttgta atactgcatg
     9721 acggaagagg cagttgatac agaaaacgga caccagaagc cgtaacccgc atgcccgctc
     9781 cgttttacgg atcctggtct ctgttctctg tcaactacgc aatcttcctg tctgcagctt
     9841 gatttatata tttaataaag atattattgc accgtcatgt aaaaataatg agcgggataa
     9901 cccgcccact gtcactttct gaggttgagc ttgtcaatga tttcacggta tctttctatg
     9961 tctttcctca tcaaatagtt cagcagacct cttctctggc caaccatctt cagcagacct
    10021 ctccttgaat ggtgatcctt tttgtgcacc ttgaggtgtt cgttgagatg gttgatcctt
    10081 tccgtgagca gcgcgatctg cacttccggc gaaccggtat cgttttcgtg aagcttgtac
    10141 ttttcgatta tttccgcttt cttttctttc tgcatcaaga ttgcctcctt ttctttttaa
    10201 ccaccgtgag ccaggtagcc ggtcggagct tccggcaacc gagcacatgg ttgaactatg
    10261 cactgatgta ttctaacata ttattcccat aaagtaaatg atatattttg aataaaaatc
    10321 agaagcctaa aaatcaaacg tccagttgta tgccttgcct gccagctttt ttactttatc
    10381 ggcaaaaccc tcggccgttt ttacatgata cgaggtctcc cctgccggtt atccgcagtc
    10441 tgtccgccgg gcgtatctgc aattcccagg ttcccgcctg tacaggatgt gaccgaagca
    10501 gtcagcagaa caactgccag cataatccat acgatccctg aaccctttat gtttttcata
    10561 gctttgcctc cccgttattt caagtcaata cctccttacg atagtttctg cgatcagaca
    10621 gtttataaca cttagtttta aaggaaattt catcctgtag aaaattttgc atgatcaatg
    10681 ttcattggcc agcaaattct atcgcagctt ttatgtatac cacgcatagc gcagtaaggc
    10741 aatgagatgc attcacaaat caggacgatg cagttcgaac atttttatgt aaacatgaat
    10801 attatataca aaaagtatat tggagggagt ctgatgttcg gaaccaacat tatgcccgag
    10861 gcatacgcaa atataccgtc ggaagttttc gccggcagca aggccaagga aaaagccgca
    10921 gcagcacaaa aaatatcttg ccacgatttc gatatgtgta aactgcagta ttacgaccct
    10981 cagcctattg tcggcgccag gcttgcacat gcctatgtac cctttcagtg cctttgctgc
    11041 ctttatccgc ctctgctggg tctcaagcag ggcaccatat tcccggaact cgacagaccg
    11101 tacggagccg atccggcata ctcgtatgac ggatgatgcc ggtttaattt tgattggagg
    11161 tgttttgcat gcatgatgcc cgcgaaaaaa tgctgaggga aatccaggct ttagaattta
    11221 tggaggtaga acttaatctt tatctcaata ctcacccgta tgaccagcag gcgctcatga
    11281 tcttcatgaa tacggcccgg cagcaaaaaa tgctgcgcga taattatgaa cgcatgtacg
    11341 gtccgctgac tgccagctca agcaattcgt tcccgtggcc ctggatcgaa agtccgtggc
    11401 cctgggtagc atgacaaaca agtacagtat tccagtcact tgaaggaggt tgtataaatg
    11461 tgggtttatg agaaaaagct gcaataccct gttaaaataa agaactgcaa tcccaggctt
    11521 gccaaataca tcatcacgca atacggagga cctgacggcg aactggcagc ttccctcaga
    11581 tacctgagcc agcggtacag catgccgacg gaagttgcaa aggcaactct caacgatatt
    11641 gggacagaag aactcgccca tctggaaata gtcggcacca tcgtatacca gcttacaaag
    11701 ggagtgcctc cgcagatcct tgagaaggaa ggcctcggcg gatattatgc cgaccacgac
    11761 aggggcatct atcccgtgaa cggggacggc gtaccgttca ccgcggcata tatcggcgtc
    11821 aagggtgatc cgatagccga tctcgtagag gatctggccg cagagcaaaa agcacgtgcg
    11881 acatatgagg gcctcatcaa tatgacagac gatcctgatg tcatagatcc tctacgcttc
    11941 ctcagggaac gtgaagtggt ccatttccag cgcttcggtg aaactaatcg ttaagatgac
    12001 aatggatata aaaccagtaa aataagtaca tacagcgagt tttaataata ccataaatat
    12061 cttatacaac agtcacgata aatgttatta gctctctgaa ggcacagagg gctaaattta
    12121 atttgaatta cctccatttt aatgtgatgc ctgctgaaat tttctgcaca ttgaattttt
    12181 tatctaacaa taacgatgat ttatatgcta aaatatttaa gacaaattga gtaagagaag
    12241 ggacaagata tgaaaaatat attaataaat aatgttgttg aagcaacttt aaaatatctt
    12301 caaacaatgc caaaagagaa gcgtaaggca attggacaat ttttcacatc agctgaaaca
    12361 gcacagtata tggcaacgat gtttgataaa ccaaaaaaga aagaattaag tatattagac
    12421 ccgggagcag gttcaggtat tttatcggca gcaataatag acaggttgca agaggatgat
    12481 actgttaaaa ggattagact tacttgttac gaaaccagtg aggacatact acctatccta
    12541 aggactaatc tacaatacat gaaagagaat tcacaaaaat ctatggatta cgaaatcata
    12601 gaagagaatt acataacttc tcaggctgcc gactttaatc aaggtatgtt tgcttcgagt
    12661 aatccgatta agtatgattg gataattgga aatccaccat atatgaaaat agctaaggat
    12721 tcatctgagg ccttggctat gcctgctgtc tgttatggtg cacccaactt gtacttttta
    12781 tttgctgcta tgagcctatt taatcttgat aaacagggtg aaatggttta tatcatacct
    12841 cgctcttgga cttctggggc ttattttaaa gcatttagag agtatttact gacggagggt
    12901 acattacgtc aggtgcattt atttgtaagc agagacgagg tctttgaaaa tgagagcgtt
    12961 ttacaagaaa caatgataat caaggtagac aagtctaata acagaggtac agtaaagata
    13021 acttcaacaa ggtccaataa ggattttaac aatataaaca ctattgaagt accttacaat
    13081 gtcattgttt atggtgttga caagtatgtt tatcttgtca ccaccaagga agaattaaag
    13141 gtactgcaaa cacttgaaaa atggaaagat acgctgcctt ctatcggcct gcgaatgaga
    13201 acgggtttaa ctgttgactt tcgcagtcga gagtttttga ggagcgagcc aggtgaagga
    13261 gtagttccct tgttttatgc acagcatatt caagctggca gagtagtttt ccccgttgga
    13321 aaagaaggtg aatatatcac tactgagcgc agtgggctaa tccagagaaa taaaaactac
    13381 cttttaatta aaagatttac gtcgaaagaa gaaaaacgac gattacaatg tgggatttac
    13441 ttggcctcta cacagccaga ctataatgcc ataagtacag ataacaaagt aaattttatt
    13501 ga
//
LOCUS       DAOSCC010000036        13233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3174_length_13233_cov_4.553346, whole genome shotgun sequence.
ACCESSION   DAOSCC010000036 DAOSCC010000000
VERSION     DAOSCC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3174_length_13233_cov_4.553346"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLG72_03355"
     CDS             <1..462
                     /locus_tag="PLG72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HOJ79870.1"
                     /translation="GIFAFMGKFGSQVDKLIWKAPSYIKLLPFFDEPYDIGQEIHMSG
                     MNRIVNVQKAFELMTLPEGKGEIVISVEDPFFPVNTGNYRIVWNDGERSVQKTDAGPD
                     LACSVQALSQLVTGFLTPDDLEFAGKVSINGKRDILAKVFRPKRLFINDYF"
     gene            complement(768..2858)
                     /gene="fusA"
                     /locus_tag="PLG72_03360"
     CDS             complement(768..2858)
                     /gene="fusA"
                     /locus_tag="PLG72_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ79871.1"
                     /translation="MKDYTAGKLRNVCLMSHGGAGKTTLAEAMLFNTGVLDRFGKVND
                     GNTTMDYDPEEVKRKISISTSIAPCEWKDHKINIIDTPGYFDFVGEVKQGIRVADSAV
                     ILVPGKGGVAVGTEKSWAYAKEQGIPRMFFVSKMDEEHANFFEVLDQLKDTFGKSVIA
                     FQLPILEGEKFVGFVDVVNMKAKKFDKDKLVDTDIPASLNDRIAEAKEALNEAVAETS
                     EELMEKYFGGEEFTAEEMINGLRAGIADCSIVPVLCGAALLNQGITALMDMIVDYMPS
                     PEYKPVIKAVKPGTDTVVELKNSADEKLSALVFKTIADPFVGKISIFRVYSGTLKSDS
                     TVYNSRAEKNEKIAQIFMLRGKKQVPADKLIAGDIGAVAKLQYTSTNDTLCDPGNQVE
                     LDKIVFPEPALSLAVEPKAKGDEEKISSGLSRLQDEDPTFRVEINAETKQTIISGMGE
                     QHLDVIVSKLKSKFGVDVNLTDPKVPYRETIRKKVKVEGKHKKQSGGHGQYGHVWIEF
                     EPGETEDLTFEEKIFGGSVPKQYHPAVEKGLREAIQHGVLAGYPVVNLKATLVDGSYH
                     DVDSSEMAFKIAARLAYKKGLPEAAPVLLEPIMHVEVYIPDSYMGDIIGDLNKKRGRI
                     LGMNPYEDGLQQVVAEVPMAEMFKYAIDLRSMTQGRGYFKQTFERYEEVPANIAQKVI
                     DEAKKTMSDEEDDD"
     gene            3359..4387
                     /gene="aroF"
                     /locus_tag="PLG72_03365"
     CDS             3359..4387
                     /gene="aroF"
                     /locus_tag="PLG72_03365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ79872.1"
                     /translation="MIIVMKPGAKESDIREVSNVLQNAGLGVHVSKGAERTIIGVIGD
                     KRLLNGIPLELMQGVDKLVPIMESYKLASRTFKPEPSTIRVGGVEFGGRQFVMIAGPC
                     AVESREQIMEAAKAVKKSGAAMLRGGAFKPRTSPYSFQGLEEEGLKMLKEASEATGLP
                     TITEVISEHSVEIASKYVDMFQIGARNSQNFHLLREVGRSGKPVLLKRGSATTIDEWL
                     NAAEYIMSEGNYNVVLCERGIRTFETATRNTLDISAVPVVKKHSHLPIIVDPSHAAGK
                     ADYIIPLSRAAVAAGADGLIIEVHPSPKCALSDAAQQLDPETFDSLCKDIGRIAEIVG
                     REFRYGAE"
     gene            4389..5480
                     /locus_tag="PLG72_03370"
     CDS             4389..5480
                     /locus_tag="PLG72_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOJ79873.1"
                     /translation="MKITIIGLGLIGGSLARAMHGKLGVQSITAVDRDSASLGLALEQ
                     GVIGKGLVTPDDSVYDSDIIFICTPIKQIIEYIDELASKVKGTCIITDVGSTKGAITS
                     HVERLPHPICFIGGHPMAGTEKSGYANSLTHLFENAYYILTPASGATEDSVRRLSSLV
                     EAIGAIPVVMTPEEHDMAVAGISHLPHVLAAALVNTVKDIDNSTGRLQALSAGGFRDI
                     TRIASSSPGLWENIVMSNRDRLLEVLKQLDRKLADFAGYLENGSSGEIYGFFDSARQF
                     RDSISDYGHGLLPRTHRIIVDVQDRPGIIGEIATLLGRHNINIKNIYVANSREFEQGC
                     LVITLQDMESMNTAFDLLTEKGYKVFRYK"
     gene            5535..6809
                     /gene="aroA"
                     /locus_tag="PLG72_03375"
     CDS             5535..6809
                     /gene="aroA"
                     /locus_tag="PLG72_03375"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ79874.1"
                     /translation="MLVENRNRLRGEITVPGDKSISHRAIVIGALASGTTEIENFLLG
                     EDCISTIDCFRRMQVSIEILKDNKVRVQGKGLYGLKAPASSLNAGRSGTAIRLLLGVL
                     SGQPFTSTVIRSEESMKKPVGNTVNFLRQMGAAITAKDNGSMCPLTVSPAKLKGISCE
                     LPIHEMHIKSPLLLAGLYAEGETVVSEPTSSRNHTELMLNYFGADIKTEGTVVRSHPV
                     ENLYAQQVTIPGDISMAAYFITAALLVPDSDIVIRNVGVNPTRSGILEVYKSMGGKIE
                     LLDKRTVSGEEVADIHVRSSRLKAVRIDEKLIPGIIDELPVIITACALAEGTTEITGL
                     FDFKVKKTGKIKAISLELSKMGARITETEDGLVIEGKESLRGTVVDSNNIHSHAMALS
                     VAGLVAQGETMIRKTQAVDIVYPAFYQTLNRL"
     gene            complement(7062..7703)
                     /locus_tag="PLG72_03380"
     CDS             complement(7062..7703)
                     /locus_tag="PLG72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOJ79875.1"
                     /translation="MASKKNVSIAVIKRLPRYYRYLSDLLKMDITRISSKELSKRMGI
                     TASQIRQDLNCFGGFGQQGYGYNVEALFIEISKILGVDKQFNCIIIGAGNMGQALAHY
                     RNFEKRGFKTIGIFDIDPRLIGKKMNDIEIMDMKDLEDFVGRNHVDIAMLTVPYEATR
                     EIAERVARLGIKGLWNFSPMDLKLPYDTVIENVHLSDSLMVLGYKIKEQFGRD"
     gene            7876..9795
                     /locus_tag="PLG72_03385"
     CDS             7876..9795
                     /locus_tag="PLG72_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ79876.1"
                     /translation="MIILSCNNISFSYGTDIILNSVSLRLQQGEKAGLVGVNGAGKST
                     LFKIITGQLKQDSGEIFISKDLRIGYLEQSSGLESDNTIWDEMLVTFSPLIKMENRIR
                     QLATEMAGEKDESSLNSMMKEYDGLLERFSREGGYEYNSRIRGVLKGLGFEESQFGLH
                     IRALSGGQKTRLALARLLLEEPDLLLLDEPTNHLDISAIEWLEDFLKNYRKSLIVISH
                     DRYFLDTVTMKTFELENNKCTTYEKNYTGYAEQKARDREIQQKHYEQQQKEIARLEAF
                     IEKQRQWNRQRNIIAAESRQKAIDRMEKTEAPDRLPDSINIRLQSSRASGNDVLSVKD
                     LSKSFPGKQLFSGINFEVKKSERLFIVGPNGCGKSTLLKILAGKLHQDSGGFKYGHNV
                     ILGYYDQELEGLDDNNTVLEEVWNGNDWLTHTQIRNVLAQFLFTGEDVCKNIGVLSGG
                     EKSRVALAKLMLSGANLLLLDEPTNHLDINSREALEEALLSYDGTIIAVSHDRYFMRK
                     LATRVVEMANGGIADYKGGYAFYLEHRRDPEADRAAGRDRAVTASKLEHLESKEDRAR
                     KRKLKKMLAECESEINRAEARLAAISVEMSREDALSDHILLTALHDEQTELENRLEEL
                     YRQWADLAEQCGDDD"
     gene            complement(9810..10523)
                     /locus_tag="PLG72_03390"
     CDS             complement(9810..10523)
                     /locus_tag="PLG72_03390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ79877.1"
                     /translation="MIIVLRKSNLILIGLIFALLLAIYSLNMDVDSQVAMTADRDAAK
                     RTVIVDAGHGGEDPGKISSYSGLKEKDLNLKIAFKVKELLEQDNYNVIMTREEDKLVY
                     KEGTTDIYYKRLQDLTRRKEIMDNSGADIVVSIHANSFTDPRYYGAQTFYPAESPESR
                     KLAECIQKSLRENVDKNNKRQPQVKQPIVIIKNLKTPTVIVETGFLSNAEEERKLATD
                     EFQDLIAKAIRKGIDDYFR"
     gene            complement(10611..11306)
                     /locus_tag="PLG72_03395"
     CDS             complement(10611..11306)
                     /locus_tag="PLG72_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79878.1"
                     /translation="MKRYISKGFKVLYTYVIADLVFVVFLYPLLGLMKERETFLRWLP
                     WYSILIFIFFFFILYIDQKELATKEKKPVYDLHPYPLKGLVYGLIGVAPLAVVTAVFK
                     IFVQFQTEIGENIKRVIINGLFGPLLFIIKWCNDAIPAYFAAMLLIPLISAFGYIAGY
                     YGINIKEKIFGKKPVQEKGFTKSPWNPSLDGNKPKKKKKKKSTGKTQSAGKNQNAANG
                     RHTDKTQNPDKTQ"
     gene            complement(11322..12257)
                     /locus_tag="PLG72_03400"
     CDS             complement(11322..12257)
                     /locus_tag="PLG72_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79879.1"
                     /translation="MFKKKILVIDDTELMVRLTTDILTKHGYEVVSANNGVDGIKMVA
                     SEKPDLVLLDVVMPGIDGFEVCKLLRKDESNNLIPIIMLTAQGNEEDKLAGLEIGADD
                     YITKPFNPRELVSRVRNTLLRIDRNRLANPLTGLPGNIDIQSEINYRIAKGMIFSVLY
                     LDVDNFKAYNDVYGFSHGDRAIKLIADILMYNVKTFGSKNDFIGHIGGDDFVVITDPE
                     HADVLCENIIKEFDERVPELYSDVDKRAGFISTVNRRGQITQYPIMTLSIAVVSNEHR
                     ELRNYLEVGEVAAELKKKAKSIVGSVYLKDKRKDK"
     gene            complement(12496..12570)
                     /locus_tag="PLG72_03405"
     tRNA            complement(12496..12570)
                     /locus_tag="PLG72_03405"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12536..12538),aa:Cys,seq:gca)
     gene            complement(12730..12804)
                     /locus_tag="PLG72_03410"
     tRNA            complement(12730..12804)
                     /locus_tag="PLG72_03410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12770..12772),aa:Gly,seq:gcc)
     gene            complement(12899..12974)
                     /locus_tag="PLG72_03415"
     tRNA            complement(12899..12974)
                     /locus_tag="PLG72_03415"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12939..12941),aa:Phe,seq:gaa)
     gene            complement(13013..13089)
                     /locus_tag="PLG72_03420"
     tRNA            complement(13013..13089)
                     /locus_tag="PLG72_03420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13053..13055),aa:Asp,seq:gtc)
BASE COUNT         3328 a         3432 c         3282 g         3191 t
ORIGIN      
        1 ggcatcttcg ctttcatggg caagttcggg tcacaggtcg ataaactgat atggaaagcg
       61 ccgtcatata taaagctgct cccgttcttc gacgaaccgt atgatatagg gcaggagatc
      121 catatgtcag gaatgaaccg catcgtgaat gtacagaagg cattcgaact catgacactg
      181 cccgaaggca agggcgaaat agtcatcagc gtcgaagatc ctttcttccc tgtgaatacg
      241 ggaaattacc ggatcgtatg gaacgacgga gagcgcagcg tgcaaaagac agacgccgga
      301 cctgaccttg cctgcagcgt ccaggcatta tcccagcttg tcaccggttt tcttaccccg
      361 gatgatctgg agtttgcagg taaggtctcg ataaacggca aaagagatat cctggcgaaa
      421 gtcttcaggc caaaaaggct gttcataaac gattatttct gataaatgtg gaaagaccgg
      481 caaagggacg gtcgagcctg ccgaaaaagt cccggacgat tcctcgaatt tgctgaaccg
      541 gacttgagaa ccgtccccgc gtctcgcggg aaccatcccc ctgtcctgga ttacgctcgt
      601 gacggacacg ctgaccctgg ctaacggttg gcgctgccaa cccccgtaac ggactttcac
      661 cgtcaaggtc gcgcgcatgc cgcgcgcaca accaaaaggg ctgcccctgc gggcagccct
      721 ctgtgtttgt tttttcaacc gatgtatcag cgatccaaac cgccaaatca atcgtcgtcc
      781 tcttcgtcgg acatggtctt ctttgcctca tcgatgacct tctgggcaat gttggccggg
      841 acttcctcat atctttcaaa tgtctgcttg aagtaacccc tgccttgcgt catgctcctg
      901 aggtcgatgg catatttgaa catctcggcc atcgggacct cggcgacgac ctgctgcagg
      961 ccgtcctcat acgggttcat cccgagtatc ctgcctctct ttttgttcag gtctcctatg
     1021 atatcgccca tgtagctgtc ggggatatat acctcgacgt gcatgatagg ctcgagcagt
     1081 accggtgcag cctcaggcag acctttcttg tatgcgaggc gtgcagcgat cttgaatgcc
     1141 atttcagatg agtcgacatc gtggtatgat ccgtcgacga gcgtcgcctt caggttgaca
     1201 acaggatatc ctgcaagcac gccgtgctgg atcgcctcgc ggaggccttt ttcaactgcc
     1261 ggatgatact gcttcggtac cgatccgccg aatatctttt cttcaaatgt caggtcttcc
     1321 gtttcaccgg gttcgaattc gatccataca tgtccgtact ggccgtgtcc gccggactgt
     1381 ttcttgtgct ttccttcgac ctttactttc tttctgatcg tctccctgta aggtaccttg
     1441 ggatcggtaa ggttcacgtc gacaccgaac ttggacttga gcttgcttac gataacgtca
     1501 agatgctgct ctcccatacc ggatatgatg gtctgttttg tctcggcatt gatctccacc
     1561 ctgaaagtcg gatcctcatc ctggagcctg ctgaggcctg agcttatctt ttcctcgtcg
     1621 ccctttgcct tgggttcgac tgcgagcgac aaagccggtt cggggaagac tatcttgtca
     1681 agctcgacct ggttgccggg atcgcacaaa gtatcgttgg tactcgtgta ctggagcttc
     1741 gcaactgctc ctatatcgcc tgcgatgagc ttgtctgcag gcacctgctt ctttcctctc
     1801 agcatgaata tctgggcgat cttttcgttc ttttccgctc ttgaattata taccgttgaa
     1861 tcggacttca gtgtgcccga gtaaactctg aatatcgata tctttccgac gaacgggtct
     1921 gctatcgtct tgaaaacaag ggctgacagt ttttcatccg cggagttctt cagttcgacg
     1981 accgtatcag taccaggctt gacagcctta ataacaggct tgtactcagg tgatggcata
     2041 taatcgacta tcatgtccat cagcgccgtg atgccctgat tcagcagtgc cgctccgcag
     2101 agcacaggaa ctatcgagca gtctgctatg cctgcgcgaa ggccgtttat catttcttcg
     2161 gcagtaaact cctcaccgcc gaaatatttc tccatcagtt cctcgctggt ctctgcgact
     2221 gcctcgttga gggcttcctt ggcctcggcg atcctgtcgt tcagagatgc agggatatcg
     2281 gtatcgacaa gcttatcctt gtcgaacttc ttagctttca tattaactac atcaacaaat
     2341 ccaacaaact tttccccttc gagaatagga agctggaacg cgataacgct cttgccgaat
     2401 gtatctttca actggtcgag gacttcgaag aaattggcat gctcctcgtc catcttgctt
     2461 acgaaaaaca ttctcggtat tccctgttcc tttgcataag cccaggactt ctcggttcct
     2521 accgcgacgc cgcccttgcc cggtaccagg attacggcgc tgtccgcaac tctgattcct
     2581 tgcttgacct ctccgacgaa atcgaaatat ccgggagtat cgatgatatt gatcttatga
     2641 tccttccatt cacagggtgc aatggaggtg ctgatggaaa tctttctctt gacctcttca
     2701 gggtcatagt ccatggtggt attgccgtcg ttaaccttgc caaacctgtc aagcactccg
     2761 gtattgaaca gcatggcttc tgccagagtc gtcttgcccg ccccgccgtg cgacatcagg
     2821 cacacgttgc ggagctttcc cgcggtataa tctttcatat gaattccccc ttgtattgtt
     2881 tacgctctaa tatattttcc tgtacttagg aaaacaattc aagaataaat gggtattatg
     2941 acacaatgta tattataaca tacatcgagg ccagttcaag gattttctta cggctgccgc
     3001 aatagtcggc cttcatttga aaaagtaata tcaacccttt aattatgaac agcggaatta
     3061 acttttgcaa atccgttttc cctgtacatg agcagttgat cccgcgctcc tttttcttga
     3121 caattatatg atatcattgt acattaaaat caaaattgcg cccgccgtaa acgcggcgcg
     3181 tttcatcagc gggcggcgtt ttcaaaccgg aagtacggca aaacggcaga acggaaacaa
     3241 taagttcagt aaaggccaat gcacgcgcaa atacacaggc cgctccactg gccggcgtac
     3301 atgcgtaggt gcgggccaaa acgtgtaaaa acgaaatacg gcagaaagga aggtaaatat
     3361 gatcatcgta atgaaacccg gcgcaaagga gtccgatatc cgggaagtct ccaatgtgct
     3421 gcagaacgcc ggtcttggcg tccatgtatc caaaggcgcc gaaaggacca tcataggagt
     3481 cataggcgac aaaaggctcc tcaacgggat cccgctcgaa ctgatgcagg gcgtcgacaa
     3541 gctggtcccg ataatggaaa gctacaagct ggcaagcagg accttcaaac ccgagccgag
     3601 cacaatacgc gtcggcggag tggagttcgg cggcaggcaa tttgtcatga tagcaggtcc
     3661 atgcgcggtg gaaagccgcg aacagataat ggaagcggca aaagcagtca aaaaatcagg
     3721 cgctgcgatg ttaaggggcg gcgcattcaa accaaggaca tcgccttact cattccaggg
     3781 gctggaagag gagggcctga aaatgcttaa ggaagcaagc gaagccaccg gccttccgac
     3841 aataacggaa gtaattagcg agcactcggt tgaaattgcg tcaaaatatg tagacatgtt
     3901 ccagataggc gcgcgcaatt cacagaactt ccacctgctg agggaggtgg ggcggtcagg
     3961 aaagcccgtc ctcctcaaga gagggtccgc caccacgata gatgaatggc tgaacgcagc
     4021 ggagtacatc atgagcgaag gtaattacaa cgttgtactc tgcgagcgcg gtataaggac
     4081 attcgagacg gctacgagga acacgctgga tataagcgcg gtcccagtgg taaaaaagca
     4141 cagccacctg cctatcatcg tcgacccaag ccacgcggca ggcaaagcag attacatcat
     4201 ccccctgtcc agggcggctg tcgcagcggg agccgacggg ttgataatag aagtgcaccc
     4261 aagcccgaaa tgtgcgctct ccgacgcggc acagcagctt gacccggaaa cgttcgacag
     4321 tttgtgtaaa gatataggaa ggattgccga aatagtaggc agagagttca gatatggagc
     4381 cgaataattt gaaaatcaca attatcgggc ttgggctgat aggcggctct cttgcaaggg
     4441 caatgcacgg aaagctgggg gtgcagagca tcacggcggt agaccgcgac agcgcgtcac
     4501 tcggactggc actggaacag ggagtgatag gaaaagggct tgttactccg gacgacagtg
     4561 tctacgactc ggatatcatt ttcatctgta ctccgataaa gcagattatt gagtatatag
     4621 acgaacttgc ttcaaaagta aagggaacat gcatcatcac tgatgttggc agcacgaagg
     4681 gcgccattac cagccatgtc gaaaggctcc cgcaccccat ttgcttcatc gggggccatc
     4741 cgatggccgg tacggaaaag tcgggctatg caaacagcct gacgcacctg tttgaaaacg
     4801 catattatat actcaccccg gccagcggag ccacggaaga ttccgtgagg cggctttcat
     4861 cgctggtgga agccatcggg gccatcccgg tggtgatgac gccggaagaa cacgacatgg
     4921 cggtagccgg gataagccat ctgccccacg tcctcgcggc cgccctggtc aatacggtaa
     4981 aggacatcga caacagcacg ggaaggctgc aggccctgtc tgcaggcgga ttcagggata
     5041 taaccaggat agcgtcctcc agcccgggcc tttgggagaa catagtaatg agcaacaggg
     5101 accggctgct tgaagtattg aagcagttgg acaggaaact ggcggacttc gccggatacc
     5161 tggaaaacgg cagttccggg gagatctacg gcttctttga cagcgcaagg cagttcaggg
     5221 attccatatc agactacggg cacgggctgc tgccacgcac ccacaggatc atcgtagacg
     5281 tccaggacag gcctggaata atcggcgaga tagcaacgtt gctgggccgt cataatataa
     5341 atattaagaa catatacgtc gcaaacagca gagaattcga gcaaggctgc ctggtaataa
     5401 cgctgcagga tatggagagt atgaatacag cttttgactt gctcacggaa aagggatata
     5461 aagtattcag atacaaatga ggatcgttta gatacaagcg atcagcaatt tttatatcag
     5521 gaggggaaaa caacgtgtta gttgagaaca ggaacagact gaggggagaa atcacagtac
     5581 ccggcgacaa gtccatatcc cacagggcga tcgttatcgg cgcgcttgca agcggtacga
     5641 ccgaaatcga gaattttctg ctcggagagg actgcatcag caccattgat tgcttcagga
     5701 ggatgcaggt cagtatagag atactgaaag acaacaaggt cagggtccag ggcaaaggcc
     5761 tgtacggact gaaagctccg gcatcctcgc tcaacgccgg ccggtcggga accgcaataa
     5821 gactgctcct tggtgttctc agtggacagc cgttcacatc caccgtcata cgaagcgaag
     5881 aatcgatgaa aaagcctgta ggaaacaccg tgaatttcct gaggcagatg ggcgctgcaa
     5941 tcaccgcaaa ggataacggc agcatgtgcc cgttgacggt atcccccgca aaactaaaag
     6001 gcataagctg tgaactgccc atacacgaaa tgcatataaa atcgccgctg ctccttgccg
     6061 gactttatgc ggaaggcgaa accgtcgtga gtgaaccgac cagttcaagg aaccataccg
     6121 aactgatgct caattacttc ggcgcagata taaaaacgga aggaacagtc gtcaggtcac
     6181 atccagttga aaatctgtat gcccagcaag tcaccatacc cggagacata tcgatggctg
     6241 cctatttcat tacagccgcg ctcctcgtcc ctgattccga tatcgtcata aggaacgtgg
     6301 gcgtcaaccc caccaggtcg ggcatacttg aagtatataa gtcgatgggc ggcaagatcg
     6361 aactgctgga caagaggacc gtctcaggag aagaggtggc ggacatccat gtccggtctt
     6421 caaggctgaa agccgtcagg atcgacgaaa aactgatacc gggcatcatc gacgaactgc
     6481 cggtcataat cacagcctgc gccctggccg aaggtacgac cgaaattacc ggcctcttcg
     6541 acttcaaggt caaaaagacc ggcaagatca aggcaatatc gctggagctt tcaaagatgg
     6601 gcgccaggat aaccgaaacc gaggacggac tggtgataga aggaaaagaa tcgctcaggg
     6661 gcaccgtcgt tgacagcaac aacatccatt cccacgcgat ggctctgtcc gtggccggac
     6721 ttgttgccca gggcgaaacg atgatcagga aaacgcaggc tgtcgacata gtctatcccg
     6781 cattctacca gacgctcaac aggctctgaa aaatgtatgg ggacactctt caactgggaa
     6841 tgcttaacat tcctcaacca tagaggaata cttctttgca gaggagcgtc gccatacctt
     6901 taacccggag tatgactgta cgaaaaccaa agcccgcata ttcattgtgc gggcttttat
     6961 ctgtatatca ttttccttta tctatatcat ttttcatttc attttttgcg ttcatcgtta
     7021 ttacaatttt tcgtccaggc cttacaactc ccgttcatat cttagtctct gccgaactgt
     7081 tccttgatct tgtaccccag gaccatcagg ctgtcgctga ggtgcacatt ttcaatcaca
     7141 gtgtcgtacg ggagtttaag atccatcggc gagaaattcc ataatccttt tatgcccagc
     7201 cgggcaaccc tctcggctat ttcgcgggtc gcttcatacg ggaccgtcag catcgctata
     7261 tccacgtggt tgcggcctac gaaatcctcg agatctttca tgtccatgat ttcgatatcg
     7321 ttcattttct tcccgataag cctgggatcg atgtcgaata tcccgatcgt tttgaaacct
     7381 cttttttcaa aattcctgta atgggccagt gcctgtccca tgttgccggc gccgatgatt
     7441 atgcagttga actgtttgtc aactcccagt attttcgata tttcgataaa cagagcttct
     7501 acattgtaac cgtagccttg ctgcccgaat ccaccgaaac aattgagatc ctgtcttatc
     7561 tgggatgcag ttatgcccat ccttttgctg agctctttgg atgagatcct cgttatgtcc
     7621 attttcaaca gatcagacaa atacctgtaa tacctgggaa gcctcttgat gaccgctatg
     7681 gaaacattct tttttgaagc catggatagc ctccttccct ttgatttcat gacatcccaa
     7741 cgacgcatca tatctacttt tgattatatt tctttgatat ttttttgtca atattgccgc
     7801 aaaacctgta ttctgctaga attatttagc ggcatatttc ccgcgtcatc catcatgttt
     7861 gaagcggtgc tgaaaatgat aatactttca tgcaataata taagtttttc atatggaacg
     7921 gacattatcc tgaacagtgt ttccttaagg ctgcagcagg gcgaaaaagc cggccttgtg
     7981 ggagtcaacg gcgcgggcaa atccacgctg ttcaaaatca taacgggaca gctgaagcag
     8041 gattcgggtg aaatcttcat atccaaagat ctgcggatcg gataccttga acagagttcc
     8101 ggccttgaat cggacaatac gatatgggat gagatgctcg tcacgttttc accgctgata
     8161 aagatggaaa accgcatcag gcagttggcc acagaaatgg ccggcgaaaa ggacgagtcc
     8221 agcctcaact caatgatgaa ggaatatgac ggcctgctcg aacgtttctc ccgtgaaggc
     8281 ggttacgagt acaacagccg tatcagggga gtgctgaaag gtctgggttt cgaagaatcc
     8341 cagttcggcc tgcatataag ggcgctgagc ggcggccaga agacacggct cgcgctggca
     8401 aggctgctgc tggaagagcc tgaccttttg ctgctggacg agcccacaaa ccacctggac
     8461 atatcggcga tagaatggct cgaggatttc ctgaaaaact acaggaaatc actgatcgtt
     8521 atatcgcatg acaggtattt cctcgataca gtgaccatga agaccttcga gctcgagaac
     8581 aacaagtgca ccacatatga aaagaactac accggctatg ccgaacagaa agccagggac
     8641 cgtgagatcc agcaaaagca ttatgaacag cagcagaagg aaatagcccg cctggaggca
     8701 ttcatagaaa aacagaggca atggaacagg cagcggaaca tcatagccgc ggaaagccgc
     8761 cagaaggcaa tagacaggat ggaaaagacc gaagctccgg acaggctgcc cgacagcata
     8821 aacatcaggc tgcagagcag cagggccagc ggaaatgacg tactgtccgt caaagacctg
     8881 tccaaaagct ttcccggcaa gcaactgttc agcggcataa actttgaagt aaaaaaaagc
     8941 gaaaggttat tcatagtagg tccgaacggc tgcgggaagt ccacgttgct gaaaatactc
     9001 gcaggaaagc tgcaccagga cagcggcggc ttcaaatacg gccataacgt catactggga
     9061 tattacgacc aggaacttga agggctggac gacaacaaca ccgtccttga agaagtctgg
     9121 aacggcaacg actggctcac ccacacccag atacgcaatg tactggccca gttcctgttt
     9181 acaggcgagg atgtctgcaa gaacatcggc gtcctcagcg gcggcgaaaa aagcagggtg
     9241 gcgctggcaa aactgatgct gtcaggcgcg aacctgctgc tcctggacga gccgaccaac
     9301 cacctggaca taaactcaag ggaagccctc gaagaagcgt tgctttcata tgacggaacg
     9361 atcatcgccg tgtcccatga ccgctatttc atgcgcaaac tggccacacg ggtggtcgaa
     9421 atggcgaacg gcggcattgc agattacaag ggcggctatg ctttctatct cgagcaccgc
     9481 agggacccgg aggcagaccg tgccgcaggc agggacaggg ccgtcaccgc ctcgaaactc
     9541 gaacacctgg agagcaaaga ggaccgggca aggaaacgaa agctcaaaaa gatgctggca
     9601 gagtgcgaaa gcgagataaa cagggcagaa gccaggctgg ccgcaatatc cgtggagatg
     9661 tcgcgcgaag atgccctgag cgaccatata ctgcttactg ccctgcacga cgagcaaacc
     9721 gaactggaaa acaggcttga agagctttac cggcaatggg ccgacctcgc ggagcagtgc
     9781 ggggatgatg actgagcatt ttttgtttat cacctgaaat agtcgtctat ccctttcctg
     9841 atcgctttgg caataaggtc ctggaactca tcagtagcta atttgcgctc ctcttcggca
     9901 ttcgaaagga aaccggtttc cacgataaca gtcggagtct taagattttt aattattacg
     9961 ataggctgct ttacctgcgg ctggcgcttg ttgttcttat ccacgttctc cctcagcgat
    10021 ttctggatgc attcggccag tttcctgctc tcaggcgatt cggccggata gaatgtctgc
    10081 gcgccgtagt accgcgggtc tgtgaagctg ttggcatgga tgctcactac gatgtccgcc
    10141 ccgctgttgt ccattatttc cttacgcctg gtcaggtcct ggagcctctt gtaatatatg
    10201 tccgtggtcc cttccttgta tacgagctta tcttcctcac gcgtcattat cacattgtag
    10261 ttgtcctgtt ccagcagttc cttgacttta aacgcgatct tcaggttcag gtccttttct
    10321 ttcaggccgc tgtaactgct tattttcccg gggtcctcgc ctccatggcc cgcatccacg
    10381 atgaccgtcc ttttggccgc atcccggtcg gccgtcatcg cgacctgcga atccacatcc
    10441 atgttcaggc tgtatatggc cagcagcagt gcaaaaatca gtcctataag aatcaagttg
    10501 gactttctca atacaataat cataaacccg tccccctatt cagattcaat ataagaatat
    10561 tcggggacgg gttggacata ttacagcgct attcaggccg tttcaggtat ttattgagtt
    10621 ttgtccggat tttgagtctt gtcagtgtgc cggccatttg ccgcattctg atttttgccc
    10681 gcactctgag tcttgcctgt gctcttcttt ttcttcttct ttttgggttt attcccgtcg
    10741 agcgacgggt tccacgggct cttggtaaag cctttctcct ggaccggctt cttgccgaaa
    10801 atcttctctt tgatattgat gccgtaataa cctgcaatgt acccaaacgc cgatatcaaa
    10861 ggtataagca gcatcgccgc gaaatatgca ggaatggcgt cattgcacca ttttataatg
    10921 aaaaggagcg ggccgaaaag gccgttgatt atcacacgtt ttatgttttc tccgatctca
    10981 gtctgaaact gtacgaaaat cttgaatacc gccgtgacga cagccagggg ggcaacgccg
    11041 atcaggccgt agaccaggcc tttgagcgga tacgggtgca ggtcgtaaac cggctttttt
    11101 tcctttgtgg caagctcctt ctggtcaatg tacaagatga aaaaaaagaa aatgaaaatc
    11161 agtatgctgt accatggcag ccatctgagg aacgtttccc tttctttcat caacccaagc
    11221 aggggataaa ggaaaaccac aaaaacaaga tccgctatga cgtaagtata caaaactttg
    11281 aaacctttgc tgatatatct cttcataaac acattctccc gtcacttgtc ttttcgttta
    11341 tccttaagat atacgctgcc tacgatggac ttggctttct ttttcagttc ggctgcaact
    11401 tcgccgacct cgagataatt gcgcagttcc ctgtgctcgt tcgatacgac ggctattgac
    11461 agggtcatta tcgggtattg ggtgatctgc cctctcctgt tcaccgtgct gatgaacccg
    11521 gcgcgtttgt ccacatctga ataaagctcg gggaccctct catcgaattc cttaataatg
    11581 ttctcgcaaa gcacatccgc atgctcggga tcggtgatca ctacaaaatc gtctccgcct
    11641 atgtgcccga taaaatcatt cttcgaaccg aaggttttta cattgtacat caatatgtcg
    11701 gctataagct tgatggcacg gtcaccatgg gaaaatccgt atacgtcgtt gtacgcctta
    11761 aagttgtcca catcaagata taatacggaa aagatcattc ccttggcgat cctgtagttg
    11821 atctcggact gtatgtcaat atttcccggc agtccggtaa gcgggttcgc cagcctgttc
    11881 ctgtcgatcc tcaggagcgt gttccttacc ctgctcacga gttcgcgggg attgaacggt
    11941 ttcgtgatat aatcgtccgc tccgatctcc aggcctgcca gcttgtcttc ctcatttccc
    12001 tgcgccgtca gcatgatgat cggtataaga ttgttgctct catccttcct gaggagcttg
    12061 caaacctcaa aaccatcgat ccccggcatc acgacatcaa ggagcacaag gtcgggtttt
    12121 tcggatgcga ccatttttat gccgtcaacg ccattgtttg ctgaaacgac ctcgtacccg
    12181 tgttttgtca gtatgtccgt ggtcagtctg accatcagtt ccgtatcatc gatcaccagt
    12241 atttttttct taaacatagc gctccccgac aaaacgcttt tcagcctgac aaccatatta
    12301 agcggcaaag cggatttgcc gtatcgttac agtacatgcc gacagacgat actgctccat
    12361 ttatatataa tatcactttt agtgcacaat acaagagaag caattaaaat atgcggcagg
    12421 cagatatccc gggacgacag gcacttgtgt attaaaataa cgaaagtcct taatacaagg
    12481 gcttccgtta ccgtttggag gcgccaccca gattcgaact ggggaataag ggttttgcag
    12541 acccgtgcct tacctcttgg ctatggcgcc gtttcgctgt gttcagttgg atgaccactt
    12601 tatcataaca tgaatgcaaa tcgatgtcaa cacctgcgag accggctaaa acacccattt
    12661 tgccgtgatg ccgcactgtt ctgcctctgc ctgacgaacg gtgcaaaaaa agcgccttac
    12721 gcgctttttt ggagcgggat acgagattcg gactcgcgac tttcaccttg gcaaggtgac
    12781 actctaccac tgagttaatc ccgcatgtat gcgtcccggt gtctccgccg cctgccggat
    12841 atgctgtgcc gcttttatca ggttttccgt ttcccgtccg cttcgggagg gtagatcatg
    12901 gtgcccagag ccggaatcga accagcgaca cgaggatttt cagtcctctg ctctaccaac
    12961 tgagctatct gggcaaatca tccacagaaa gcgctgcctt ctatggatta agtggcgacc
    13021 cggaagggac tcgaaccctc ggcctctagc gtgacaggct agcgttctaa ccaactgaac
    13081 taccgggcca catttctgcc gctctccgat ttcaggacct cttctccgga agcctcacgg
    13141 ccatcccaga gagaaatcgc tggtgggcac tatagggctc gaacctatga ccccctgctt
    13201 gtaaggcagg tgctctccca gctgagctaa gcg
//
LOCUS       DAOSCC010000037        13112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3208_length_13112_cov_4.561308, whole genome shotgun sequence.
ACCESSION   DAOSCC010000037 DAOSCC010000000
VERSION     DAOSCC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3208_length_13112_cov_4.561308"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLG72_03425"
     CDS             complement(<1..640)
                     /locus_tag="PLG72_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79880.1"
                     /translation="MPVVRSHHKLFFENQDTAGKVSHDINTIISGITNDILRECPSLV
                     ADVSAGRAPRSALETVIIKDMDLHRYHHGIDRDELIRKVMDFMFGYGLLQPYIDDEDI
                     TDIDGTNYDQFVIKRHGVRSKIPVNFGSEKIFDTYCKLVAIRNGGILNENDSHCRVTD
                     EKNRLRINVSIKPRNISSPAISIRKHRKTSYTIDDLKRLGMLDDEVAALLRDL"
     gene            complement(645..2564)
                     /locus_tag="PLG72_03430"
     CDS             complement(645..2564)
                     /locus_tag="PLG72_03430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ79881.1"
                     /translation="MQAGDYFAEKYLIEGILGRGGMGTVYLAKNVKTGSHWAVKEIGN
                     GQVTDTGMPSEASLLARLDHPALPRLYDLVEQDGKLYMITDYIDGISLDRKLEAEGRI
                     AEDTVIDWAIQLCMVLDYLHSIKPNPVIYRDIKPSNIMLTGSGMLKLIDFGTAREYKP
                     QSDADTVYIGTRGYAAPEQFGTGQTSAVSDIYSLGVTLHHLVTGKSPVEPPFRLEPIR
                     SYDPSLSLELEAVILKCTCESPSGRYQSAAELLADLDILRERKSHVPDRDAGSGPYRS
                     MGCIPYQDRRLFTGRIGFPASGLAECAEKEHDGIGETFADMEGNIRDSENKRRERNNE
                     MESQITASVPDDADHESNTPVFSFRRMVITVWDNAEFGCELAYTAAKLTGSDVLLADL
                     DLLAPKADLILNVGKYPLQLAKHGIPGRSGLDIIMDAAGKRMLTSELLRRAAETRKEM
                     KNLYIITGNYRLDNYEYYSEDSVTYLIDKCYRYFDITVLLVNRSIYDAFTLAALLRSD
                     VNIAAVKGETGQLREFNTYIAFLDEKQHLPPENTKFVLFEYDGTSDISKAEAAQATQG
                     NLLGTVSFSRKRVLYRNMNSAYVCHMEKDILRDYSRIHKKLGFMPSAGFMPVLKIFGN
                     LAGGMNRKKSRPMEG"
     gene            2919..3887
                     /locus_tag="PLG72_03435"
     CDS             2919..3887
                     /locus_tag="PLG72_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ79882.1"
                     /translation="MKITGVTYAVRKTHRTRYVVLTVVILLLVAIAAVIALSVYESWQ
                     LLHPDKKPIDAFSSNIVPEYRDITFRGADSTIVLKGWLFGVKNTNRAVIFVHSYGKNR
                     LEFGVDTVDLIKEFMNRGYSVLTFDLRNSGESGGKDCTFGYNEKEDVKAAIKYVRDQG
                     YSDITLLGFSTGASAAIMACAESTNADGTAMVDAVIADSPYSDLKSYFLSSLHRWTGI
                     PSFPFNRTISFAFSVTGGFKMKDASPVDVMTAENLPHLMLIHSRNDDLVPVINSIELY
                     QKYSALNASGAEFWQTEYKGHASGYMENRDEYIERIFSFLDKVYRD"
     gene            complement(4086..4574)
                     /locus_tag="PLG72_03440"
     CDS             complement(4086..4574)
                     /locus_tag="PLG72_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOJ79883.1"
                     /translation="METDNRNYFARMTELLARKKVLLAEMLELTRDQTDAIDEKSLDR
                     LQKLIEEKQKRIDEIDKLDVEFAACMKRLKAAVGVKELSELDASRFPGARELKLATAE
                     VLSLVEEISGAEKVNSAKSKELLDELGRQVRRLNQAKKLNNAYNRQDAGGAPSFFLDK
                     KK"
     gene            complement(4669..5073)
                     /gene="fliS"
                     /locus_tag="PLG72_03445"
     CDS             complement(4669..5073)
                     /gene="fliS"
                     /locus_tag="PLG72_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOJ79884.1"
                     /translation="MVTSNAYDTYKQNAIMTASPEELTLMLYNGLIKFMMVARKAIEE
                     KDIPKAHESIIRAQDILNEFKATLDMQYEISHNLNQLYDYFLDRLFEANIKKDTAILD
                     EVLRFVRELRDTWAQAMKIAKQQNKKAAGANE"
     gene            complement(5108..7348)
                     /gene="fliD"
                     /locus_tag="PLG72_03450"
     CDS             complement(5108..7348)
                     /gene="fliD"
                     /locus_tag="PLG72_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ79885.1"
                     /translation="MSYIYTSYNPTTRITGLSGLDVDGLVYQLMQVERLKVDSVSKSR
                     QLLVWKQEQYREIISALQSFSNEYFNSLKPATDMRNSNIYNAFEIKYGGQDTSPYFTA
                     SAASGARAGSYIIRNIRTAKTARVLGAEVAGHLTGKPLESVNISGEKGNNVFDVEFNG
                     VKKNIVLNDNLTDINSLRADLQSKLDEAFGSGKITVRADGGRISFETSSSNTLTFSNT
                     VNDGYAAIFNKDLSSGITLTGLNNKFRITLGSEYKDFELPAGEYTDANALAAVLQTLV
                     DDQEFGFGEGKVTVKVDNNKIVLESTDPDVKVSASAVQNSGLEAIGLGGINNSSKINL
                     DANIYDIKDRFAIPLGISDGNTEISFEINGQTFTFDAKTTSLNKIMAAVNSNTAAGVR
                     MSYDSLNNMFILETKTTGATASIMATDNSGGLLASLGLTTNEAVRGEDASITYIDPTV
                     TYFDPEDKKEKPVEHVITRSTNSFNINGIVFNLQKDFEGEVELSVKSDPTKAVELIKG
                     FVEKYNELLGKINAMLSEERDYKYEPLTDEQKDAMTEKDIELWEKKAKSGLLNGDNLL
                     RSIVTGFRNAVISSVEGTGLTLASIGIKSNSWVDRGKLYVDEEKLKQALAENPTEVFE
                     LFTKQSDVSYNSAVGSASARNQRFNESGLIYRLYDVIQDNIRTTTINGHRGALLEKAG
                     MTGDRSLYNNTLYQQIAAYDKEIAELNEELIMKENQYYIQFAQLERLISEMNNQSLWL
                     ARQFGF"
     gene            complement(7355..7732)
                     /locus_tag="PLG72_03455"
     CDS             complement(7355..7732)
                     /locus_tag="PLG72_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOJ79886.1"
                     /translation="MRVQGMDATAGMKVGNEPQFFGNRAADAVQVKTGQERVAQQKKI
                     ETAEYPINERVVSEAVDKMNKVVEGYNRRFEYKIHEGTNDIMIRVIDETTKEVIREIP
                     PKKILDMIASMLEMAGLMVDERR"
     gene            complement(7818..9140)
                     /locus_tag="PLG72_03460"
     CDS             complement(7818..9140)
                     /locus_tag="PLG72_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ79887.1"
                     /translation="MDNIDIKTDLLNKISNKTAVVGVIGLGYVGLPLAVEKARAGYKV
                     IGFDIQEQKVRMVNNGVNYIGDVVQADLKKLVESGRLKATGDYSFVADVDCIAICVPT
                     PLDEYSQPDISYVVNSAKEMAEYLHRGMLVVLESTTYPGTTEEVLMPILESTGLKCGT
                     DFYLAFSPERVDPGNKIYKTKNTPKVVGGVTPDCTEVAAALYNNVLDNEVFRASSPRV
                     AEMEKILENTFRNVNIALANELAIICDKMGIDVWEVIEAAKTKPYGFMPFYPGPGVGG
                     HCIAVDPFYLTWKAREYGYHTKLIETAGEINNYMPEFVLERAMKILNRFGKPLNGSEI
                     LVLGAAYKQDIDDVRESPALKVMDLLVRHGAVLKYNDPYVPKFTYKGRSYESVPLTAE
                     ALEAADLVIITTMHGCYDYEFIEKHSRFIFDTKNAMKNIKNRAKIETL"
     gene            9335..10372
                     /locus_tag="PLG72_03465"
     CDS             9335..10372
                     /locus_tag="PLG72_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ79888.1"
                     /translation="MKDIINFGLVGCGRISKNHIEAISMLKDSARLVAVCDIDPVRAQ
                     KTGEENSVDYYTCLDDMLARDDLDAVSICTPSGLHPAHGIAAAKRKLHVITEKPMATS
                     LKKADELISACDENGVQLFVVKQNRLNPTLQLVKRAIDKGRFGRIYMAIVNVLWSRPQ
                     SYYDQAKWRGTWEFDGGAFMNQASHYIDMIEWLIGPVESVCAYTGTLARRIETEDTGT
                     ACIKFRSGAMGSINVTMLTYPKNFEGSLTIIGENGTVRVSGIAVNKIEHWEFRDYDDD
                     DKLVGMSSYNPPNVYGYGHTGYYKNVIDTLKGIAEPDTDGREGRKSLELILAIYKSAR
                     EGRSIPLPLEY"
     gene            10446..11021
                     /locus_tag="PLG72_03470"
     CDS             10446..11021
                     /locus_tag="PLG72_03470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ79889.1"
                     /translation="MKDYFAHESSYVDEPCEIGEGTKIWHFSHIMAGCRIGRNCNIGQ
                     NVVISPDVVIGDNVKIQNNVSVYTGVVCEDDVFIGPSAVFTNVINPRSHINRKNEFRK
                     TIIRKGASIGANATIVCGHEIGMYAFIGAGAVVTRNVPDFALVVGNPARHAGWMCRCG
                     SRLDFSNDKNTAVCRECGSEYIMEGSRVRLK"
     gene            11088..12233
                     /locus_tag="PLG72_03475"
     CDS             11088..12233
                     /locus_tag="PLG72_03475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ79890.1"
                     /translation="MNIPLLDLKAQYSQIKDEIRQAVDEVLESQQFILGPRVSELESE
                     IASYSRCKYALGVSSGTDALLLALMALGTGHGDIVITTPYTFFATAGCIARLGAKPVF
                     ADIDPATFNIDPRKLETLLEKMTSEELSRVRAVIPVHLFGQMADMQSIVPICRKYGLK
                     IIEDAAQAIGSECPFGGGVQRAGSIGDIGCFSFFPSKNLGGIGDGGMVVTNDADLYEK
                     MKILRVHGSSPKYYHRAVGGNFRLDEIQAAVLLVKLKYLDTWTEARQKNARIYDELLS
                     GVVSEHFRTPAVLPGYRHIYNQYCIRTAKRDDLKVHLRENGVATEIYYPLPMHLQECF
                     RYLGYKKGDFPEAEEAALTSLALPVYPEMGADGLEYTADLIRNFIMR"
     gene            complement(12257..>13112)
                     /gene="rfbG"
                     /locus_tag="PLG72_03480"
     CDS             complement(12257..>13112)
                     /gene="rfbG"
                     /locus_tag="PLG72_03480"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOJ79891.1"
                     /translation="VSPGLQRVEYPLAAQPLVRVSYDRPAETYETNVMGTVNLLDAIR
                     RCPSVKAVVVVTSDKCYENTESSRGYRETDRLGGYDPYSSSKACQELVTSAYINSFFN
                     TAEYSRHGVAIATARAGNVIGGGDFAQDRLVPDIIRSIISGEQLRIRYPRAVRPWQHV
                     MEPLFGYMTLAQKLYTDGPAYSGAWNFGPDQGSIKTVEWIVGRLLDYFGREYGYVADT
                     ENNPHETNCLMLDSAKAKQLLGFRPRLDIEKALEKTAYWTTAFLDKADMMRVTLEQID
                     EYENIGNP"
BASE COUNT         3034 a         3370 c         3081 g         3627 t
ORIGIN      
        1 ccagatccct gagcagggct gcgacctcat cgtcaagcat gccgagccgt ttcagatcat
       61 ctatcgtgta gctggttttc ctgtgcttgc gtatgctgat ggccgggctg gatatgttcc
      121 ttggtttgat cgaaacattg atcctgagcc tgtttttctc atcggtcacc ctgcagtggc
      181 tgtcgttttc gttcaggatc ccgccgttgc ggattgccac cagcttgcag tatgtgtcga
      241 atatcttttc actgccgaaa ttgacaggta ttttacttct tactccgtgc ctctttatga
      301 caaactggtc ataattggta ccatcgatat ccgtaatatc ttcgtcgtca atatatggct
      361 ggagcaggcc gtatccgaac atgaagtcca tgaccttcct tatcagttcg tccctgtcta
      421 ttccgtggtg gtatctgtgc aaatccatat cttttatgat caccgtttcc agtgcgctcc
      481 tcggagcacg ccctgctgat acgtccgcca ccagggacgg gcattccctc agtatatcat
      541 tggtgatgcc ggatatgatc gtattaatat cgtgactgac tttcccggca gtgtcctgat
      601 tctcaaaaaa cagcttatga tgcgacctga ccacaggcat atcgttatcc ctccataggc
      661 ctgctctttt tcctgttcat gccgccggcc aggtttccga atattttcag gacaggcatg
      721 aaacccgccg acggcataaa accgagtttt ttatgtattc gggagtaatc cctgaggatg
      781 tctttttcca tatggcacac atatgcactg ttcatgtttc tgtataaaac gcgtttcctg
      841 ctgaatgaca ccgtccccag cagattgccc tgcgttgcct gcgcagcttc ggctttgctg
      901 atatcagatg tcccgtcgta ttcaaacagg acgaatttgg tgttttccgg cggcagatgc
      961 tgtttttcgt ccaggaacgc aatataagtg ttgaattcgc gcaattgtcc tgtctcgccc
     1021 tttaccgccg caatattgac gtccgatctc aaaagcgctg ccagcgtgaa cgcatcatag
     1081 atcgaacggt tcacaagaag cacggtgatg tcgaaatacc tgtagcattt gtcaatgaga
     1141 tatgtaacgc tgtcctcgct gtaatattcg taattgtcaa gccggtaatt ccctgtgatg
     1201 atataaaggt ttttcatctc ttttcttgtc tcagcagccc ttctcagcag ttcggacgta
     1261 agcatccttt tccctgcggc atccatgatg atgtcaagtc ccgaacggcc gggaatgcca
     1321 tgctttgcca actgcagcgg gtatttgccc acattcagta tgagatcggc tttgggcgcc
     1381 agcaggtcga gatctgcaag gagcacatcg cttcccgtca gctttgccgc agtatatgca
     1441 agctcacatc cgaactctgc attgtcccat actgttatga ccatccttct gaaactgaat
     1501 accggggtat tgctttcatg atctgcatcg tccgggacag atgcggtaat ctgagattcc
     1561 atttcattat tcctttctct tcttttattt tcactgtccc tgatgttccc ttccatgtcc
     1621 gcaaatgttt ctccaatccc gtcatgttct ttttcggcgc attctgcaag gccggatgca
     1681 ggaaacccga tcctgccggt gaacagccgg cgatcctggt acggtatgca tcccatgctc
     1741 cggtatggac cggatccggc gtccctgtcc gggacatggc ttttcctttc acggagtata
     1801 tccagatcag caagcagttc tgccgcactt tggtatcgtc ctgaaggcga ttcgcacgta
     1861 cacttcaata taacggcctc aagctcgagg gaaaggctgg gatcatagga ccttatgggt
     1921 tcgagcctga atggcggctc gaccggactt ttgcccgtca caaggtggtg cagtgtcacc
     1981 ccgaggctgt agatgtcaga taccgcactg gtctggccgg tgccgaactg ctcgggagcc
     2041 gcatatccgc gggtgccgat atacacggtg tcggcatccg actgaggctt gtattcgcgt
     2101 gcagtgccga aatctatcag cttaagcatg ccgctccccg tcagcattat gttggatggc
     2161 tttatatccc tgtatatcac aggattgggt tttatcgaat ggagatagtc cagtaccatg
     2221 caaagctgta tggcccagtc gataacggtg tcctccgcta tccttccttc tgcttcgagt
     2281 ttcctgtcca gcgagatgcc gtcgatataa tccgttatca tatatagttt tccgtcctgc
     2341 tcaaccaaat cgtataacct cggcagcgcc ggatgatcca gcctggcaag caggcttgct
     2401 tctgacggca tccccgtatc ggtgacctgc ccgttgccta tttccttgac cgcccaatga
     2461 cttcctgtct ttacgttctt tgcaaggtat acggtaccca tcccgcctct gcccagtatc
     2521 ccctcgataa ggtatttttc agcaaagtaa tctcccgcct gcataatgat ccctcacacc
     2581 agcaaatgca tactgttgtc gtttgatgta cattctttgg ttgttatttt tttacatatt
     2641 ctcgtgactg attttatcat aacctatatc caaaatctat acatgctgta aatattttct
     2701 tgctttgtaa tataatatct tgccaaatgt agattttaag cagaaaatga agtatgcaga
     2761 aaatgtatta ttcgggaaat aatcttcggt acggagtgca ttttgcggtc cggacagtgc
     2821 cgtttggtga gcctttactt tacatataat aaaaaatata ataaaggtaa acataaggtg
     2881 taccgaacct ggaaagcaat atggaggtat ctggaaatat gaagattacc ggagttacat
     2941 atgcagtgag gaaaacgcac aggaccaggt acgtggtact gactgtggtt atccttcttc
     3001 ttgtcgcgat agcggccgtg atagctttgt ctgtttatga aagctggcag ttgctgcatc
     3061 cggacaaaaa gccgatagat gcattttcct ccaatatagt gcctgaatac agggatataa
     3121 ccttcagggg agcagacagc acgatagtgc tgaaaggttg gcttttcggg gtcaaaaaca
     3181 ccaaccgtgc ggtcatattc gtacattcat acggtaaaaa caggcttgag ttcggagtgg
     3241 ataccgtgga tctgatcaag gaattcatga accgcggcta cagcgtactt accttcgatc
     3301 tgaggaactc aggcgaatca ggcggtaagg actgcacttt cggatataac gaaaaagaag
     3361 atgtgaaagc tgccatcaaa tatgtaagag accagggtta cagcgatata acgcttttag
     3421 gtttttctac gggagcgtcc gcggcgatca tggcatgcgc tgaaagcacc aatgcagacg
     3481 gtactgcgat ggtcgacgca gtcatcgccg acagtcccta ctcggatctg aaaagttact
     3541 ttctcagcag tctgcacaga tggacaggca tcccttcttt cccgttcaac aggacgatat
     3601 catttgcgtt cagcgttacc ggcgggttca aaatgaagga cgccagtccg gtcgatgtca
     3661 tgacggccga aaacctgccc catctgatgc tgatacacag ccgcaatgac gacctggtac
     3721 ctgtcataaa ctcaatagaa ttgtaccaga agtattccgc gctgaacgct tccggcgccg
     3781 aattctggca gactgagtac aaagggcacg ccagcggcta tatggaaaac agggatgaat
     3841 atatcgaaag gatattctcc ttcctcgaca aggtttatcg ggattgaccg gtcctgtata
     3901 acggctccga ctgcaaaccc cgaccattca tcaggaccgg gccggttcag ttggtaaacc
     3961 agctgaagtt atattggaag agaaccaagg cagaggaatt ctggccagac aagtattaga
     4021 caactttagc ataaatcaat ataaaaagcg ggtctgtctg acccgctctt ttttttacga
     4081 cccgctcatt tctttttgtc aaggaaaaac gaaggggctc cgccggcgtc ctgcctgttg
     4141 tatgcgttat tcagcttttt cgcctggttc agcctcctga cctgcctgcc gagttcatcc
     4201 agaagttcct tgctctttgc gctgttgact ttttcggcgc cgcttatctc ctcaacaagc
     4261 gaaaggacct cggctgtcgc cagcttcagt tccctggccc ctggaaaacg ggatgcgtcc
     4321 agttcactga gttcctttac gccgacagcc gctttcagcc ttttcatgca agcagcgaat
     4381 tccacatcca gcttgtcgat ttcatctatc cgtttctgct tttcttctat caatttctgc
     4441 agcctgtcca gcgatttttc gtctatggca tcggtctggt ccctggtcag ctccagcatt
     4501 tcggccagca ataccttttt ccttgccaga agctccgtca tcctggcgaa atagttcctg
     4561 ttatcggttt ccataacccc tcgtctatta tagagacttg cctgcgattc atttattccg
     4621 ttaatgcata tgcagtgata ttttcagcag catcgtgctg ttcacgttct attcgtttgc
     4681 ccctgccgcc ttcttgttct gctgttttgc gatcttcatc gcctgtgccc atgtatcccg
     4741 gagttccctg acaaagcgga gcacctcgtc cagtattgcg gtgtcctttt tgatgtttgc
     4801 ctcaaaaagc ctgtcaagga aatagtcgta caactggttc aggttgtgcg atatttcata
     4861 ctgcatgtcg agcgtcgcct tgaactcgtt caggatatcc tgcgccctga ttatgctctc
     4921 atgggctttg gggatatcct tctcttcaat agccttccgg gctaccatca tgaatttgat
     4981 cagcccgttg tatagcatta gggtcagttc ttccggactg gcggtcatga tcgcgttttg
     5041 cttgtaagta tcatatgcat tcgatgtaac catttccaca ccttctgcct ttccgtttta
     5101 tgttgattta aaagccaaac tgccgtgcaa gccacaaact ctggttattc atctcgctga
     5161 tcagtctctc cagctgtgcg aactggatgt aatactggtt ttccttcata atgagctctt
     5221 cattcagttc cgctatctct ttgtcataag ctgctatctg ctgataaagc gtattgttgt
     5281 acagcgaccg gtcaccggtc attcccgctt tctccagcag agctcccctg tgaccgttga
     5341 tcgtggtcgt ccggatgttg tcctggatta cgtcatatag cctgtatatg agtccggatt
     5401 cattgaacct ctggttcctc gcacttgcac tgccaaccgc cgaattgtat gaaacatccg
     5461 actgtttagt gaacagttcg aaaacttccg tagggttctc ggccagggct tgctttagct
     5521 tttcttcatc aacataaagc ttgcccctgt ccacccaact gttggatttt atccctatac
     5581 tcgcaagcgt aaggccggtg ccttcaacac ttgaaataac ggcattacgg aaacctgtaa
     5641 caatggatct gagaaggttg tcaccgttga gcaaccccga ttttgccttt ttctcccaca
     5701 attctatgtc tttttccgtc atcgcatcct tctgttcgtc cgtcaacggc tcatatttat
     5761 aatctctctc ttcgctcagc atggcattga tcttgcctag caattcgttg tatttttcca
     5821 cgaaaccctt gatcagttcg acagcctttg taggatcgga ttttaccgac agctcgacct
     5881 caccttcaaa gtctttctgc aggttgaata cgatcccgtt tatattgaag ctgttggtag
     5941 atcgtgttat aacgtgttca acaggcttct cttttttatc ttcgggatcg aaataggtca
     6001 cggtcggatc gatataggtt atgctcgcgt cctcgcccct gaccgcttca ttggtcgtca
     6061 gccccaggga agccagcaat cctccactgt tgtcagttgc catgatgctg gcggtcgcac
     6121 cggtcgtctt cgtttccagt atgaacatgt tattgaggct gtcatagctc atcctgacgc
     6181 ccgcagccgt gttcgaattg acagcagcca ttatcttgtt cagggatgtc gttttcgcat
     6241 cgaatgtgaa agtctggccg tttatctcaa agcttatttc ggtattgccg tcactaatgc
     6301 caagaggtat tgcaaacctg tcctttatat cgtaaatatt ggcatcgaga tttattttgc
     6361 tggaattgtt gataccaccc agcccgattg cctcgagccc gctgttctgc accgcggatg
     6421 ccgagacttt gacatccggg tcagtgcttt ccaggactat cttgttatta tcaactttaa
     6481 ctgttacctt tccctctccg aacccgaact cctgatcatc caccagggtc tgaagtactg
     6541 cggccaaagc atttgcatcc gtatattctc ctgctgggag ttcaaagtcc ttgtattctg
     6601 aaccgagggt gatcctgaac ttgttgttca ggcccgtcag tgttatcccg gaactcaggt
     6661 ccttgttgaa tatcgcggca tatccgtcat tcacggtgtt ggagaatgtg agggtattcg
     6721 aactgcttgt ctcgaatgaa atcctgcccc catccgctct cactgtgatt tttccgcttc
     6781 cgaacgcttc atccagcttg ctttgcagat ctgcccgaag cgagtttatg tcggtcagat
     6841 tgtcgttaag gacaatgttt ttcttgacgc cattgaattc aacgtcaaat acgttgttgc
     6901 ccttttcacc gcttatattt accgattcga gcggcttgcc cgtgaggtgt cctgcaactt
     6961 ctgcgccaag taccctggcg gttttggcgg tcctgatatt cctgattata tagctgcccg
     7021 ctcttgcgcc tgaagctgcc gaagccgtga aataagggct cgtgtcctga ccaccatatt
     7081 tgatctcgaa tgcattgtaa atgttggagt tcctcatatc ggtcgcaggc ttcagtgaat
     7141 tgaaatactc gttactgaag ctctggagcg cgctgattat ctccctgtac tgctcctgct
     7201 tccataccag cagctgcctg ctttttgaca cggaatccac tttgagcctt tcgacctgca
     7261 tcaactgata aaccaggccg tccacatcca agccggacaa tccggtgatc cttgtggtgg
     7321 ggttgtatga tgtatagatg taactcatca gaaatcacct tctctcgtct accataagcc
     7381 ctgccatttc gagcatgctt gctatcatgt ccagtatctt tttcggaggt atttccctga
     7441 tgacctcttt ggtagtctcg tcgatgaccc tgatcatgat atcatttgta ccttcatgta
     7501 tcttgtactc gaatctcctg ttgtatccct ctaccacttt gttcattttg tccacagctt
     7561 cagaaactac ccgttcattt atgggatatt cagctgtctc aatctttttc tgctgtgcaa
     7621 ctctttcctg acctgtcttc acctgtacag catccgcagc cctgttgccg aaaaactgcg
     7681 gttcattgcc gactttcatg ccggctgtgg catccatgcc ctgaaccctc atataaatcc
     7741 ctccatgaaa tcaccaagtt aaacataatc tctcttctta tttatcggaa taatatagaa
     7801 aaggatttat atgtttttta aagtgtctct attttcgctc tgttcttgat atttttcatt
     7861 gcattttttg tgtcgaaaat gaacctgctg tgcttttcta taaactcata atcataacaa
     7921 ccgtgcatcg tcgtgatgat caccagatcc gcagcctcca gggcttcagc ggtcagaggc
     7981 accgattcgt aggatcttcc tttgtatgta aatttaggta catacgggtc attgtacttc
     8041 aggacagcgc catgcctgac aagcagatcc atgactttga gcgccggcga ttccctcaca
     8101 tcatcgatgt cctgcttgta tgccgcgccg agcaccagta tttcggaacc attgagcggt
     8161 ttgccgaagc ggttcagtat cttcatcgct ctttcaagca cgaattccgg catataattg
     8221 ttgatctctc ctgcagtttc tatcagcttt gtgtggtaac cgtactccct ggctttccat
     8281 gtgagataaa acggatcgac agctatgcag tgccctccga cacccggtcc cgggtaaaaa
     8341 ggcataaatc cgtacggttt ggttttcgcc gcttcgatta cctcccagac atctatgccc
     8401 attttatcgc atatgatcgc caactcgttc gccaatgcaa tgtttacgtt cctgaacgta
     8461 ttttccagta ttttctccat ttccgcgacc ctcggcgacg atgccctgaa cacttcgttg
     8521 tcgagcacat tgttgtaaag cgcggcagcc acttcagtac aatcaggagt tacccctccg
     8581 actactttcg gcgtattttt tgttttatat atcttgttcc ccgggtctac acgttccgga
     8641 gaaaaagcaa gataaaaatc tgttccgcat ttgagcccgg tggattccag tatcggcatc
     8701 aaaacctctt cggtggtccc cggatatgta gtgctttcaa gtacgaccag catgcctctg
     8761 tggaggtatt ctgccatttc tttcgctgaa tttacgacat acgatatatc aggctgggaa
     8821 tattcatcca gcggtgtcgg tacacaaatg gctatgcagt ccacatcagc gacaaagctg
     8881 taatcgccgg tcgccttcag tctgcctgat tccaccagtt ttttcagatc tgcctggact
     8941 acatcgccaa tataattgac tccgttattg accatcctga ccttctgttc ctggatatcg
     9001 aagccgatga ctttgtatcc cgccctggcc ttctccaccg ccagcgggag tccgacatat
     9061 ccaaggccga tcactccgac gaccgcagtt ttattgctga tcttgttcaa aaggtctgtt
     9121 ttgatatcaa tattgtccat gcatacccgc cgccttatca tatactcgtt ctatatatcg
     9181 gctttataca gaatttacat cataacgtat ttcatcaaaa gccgggaagt ctttttcggc
     9241 agccaatgcc gaagttaacg gattcgacgg ttttgccgat aagatttata tgacggcaca
     9301 gtcagaagct gcagtatcaa gaggtgaatt ttcaatgaaa gatatcataa actttggtct
     9361 ggtgggctgc ggcaggatat caaagaacca tatcgaagcg atttcaatgc tgaaagacag
     9421 tgccagactt gttgccgtat gcgatataga cccggtaaga gcgcaaaaga cgggagaaga
     9481 gaattccgtt gactattaca cctgccttga cgacatgctt gccagagatg atctggatgc
     9541 agtgtcgatc tgcactccaa gcgggctcca tcctgcacat ggtattgccg ccgcaaaaag
     9601 aaaactccac gtgataaccg aaaaacccat ggctaccagc ctgaaaaagg cggatgaact
     9661 gatatcggca tgcgacgaaa acggcgtcca gttgttcgtg gttaagcaaa accgtctcaa
     9721 ccccacgctt cagcttgtaa aacgcgcaat tgacaaaggg cgcttcgggc ggatatacat
     9781 ggccatagta aatgtgctgt ggtcaaggcc gcaatcgtac tacgaccagg ccaaatggcg
     9841 cggcacatgg gagttcgacg gcggtgcctt catgaaccag gcaagccact atattgatat
     9901 gatagaatgg ctcatagggc ctgtcgaaag cgtgtgcgca tataccggca ccctggcgcg
     9961 ccgcatcgag accgaagaca ccggcaccgc ctgcatcaag ttcaggagcg gggccatggg
    10021 gtcgatcaac gttacgatgc tgacataccc gaaaaacttt gaaggatcgc tgaccattat
    10081 aggagaaaac ggcaccgtca gggtaagcgg aattgcagtc aataaaatag agcactggga
    10141 attccgcgat tacgatgacg acgacaagct ggtcgggatg agttcataca atcctcccaa
    10201 cgtatatggc tacgggcata caggctacta caagaacgtg atagatacgt taaagggaat
    10261 tgcggaacct gataccgacg gcagggaagg ccggaaatcg cttgagctta tccttgccat
    10321 atataaatcg gcaagggagg gccgaagcat cccgcttccg ctggagtatt agggtatggg
    10381 gacactcctc tgcaaagagg cataccatag cggatgagga atgtcctatt cttcaggagg
    10441 gcaatatgaa ggattatttt gcacatgaat caagttacgt ggatgaaccc tgcgaaatag
    10501 gcgaaggaac caaaatatgg cacttctctc acataatggc cggctgcagg atcggacgta
    10561 actgcaacat cgggcagaac gtggtaatat cccccgacgt cgtcataggt gacaatgtca
    10621 agatccaaaa caatgtttcg gtctacaccg gcgtagtttg cgaagatgat gtcttcatcg
    10681 ggccttccgc ggttttcacc aacgttataa accccaggag ccatataaac aggaaaaatg
    10741 aattcaggaa gaccattata aggaaaggcg ccagcatagg cgccaacgcg actatcgtct
    10801 gcgggcatga aatcggcatg tacgcattca tcggggccgg agcggtggtc acaaggaatg
    10861 tcccggattt tgcgctggtc gtcggcaatc cggcaaggca tgcaggctgg atgtgcaggt
    10921 gcggttccag gctggacttc agcaatgaca agaacaccgc agtttgccgc gagtgcggca
    10981 gcgaatacat tatggagggc agcagggtcc gtctgaaata gacaggtttg aaaaaatata
    11041 accgggcata tttgttcatg caaaataata aaatgggagg cagcgtcatg aatatccctc
    11101 tcctggatct caaagctcag tattcgcaaa taaaggatga aataaggcag gctgtcgacg
    11161 aagtactgga atcgcagcaa ttcatactcg gtcccagggt gtccgaactg gaatcggaga
    11221 tagcttctta cagccggtgc aaatacgccc ttggcgtgtc ttcaggcacc gatgcgcttc
    11281 tgctggcgct gatggccctg ggcacaggcc atggagatat cgtcattaca acgccttaca
    11341 cgtttttcgc aacagcgggc tgcattgcaa ggctcggtgc aaaaccggta tttgccgata
    11401 tcgatcccgc caccttcaat atcgatcccc ggaagctgga aacactcctg gaaaagatga
    11461 caagtgaaga actgtcccgc gtcagggccg tcatacctgt ccacctgttc gggcagatgg
    11521 ccgatatgca aagcattgta ccgatctgcc gaaaatacgg cctgaaaata atagaggacg
    11581 cagcccaggc gatcggttcc gagtgcccgt tcggcggcgg tgtgcaaaga gccgggagta
    11641 tcggcgatat aggctgtttt tcgttcttcc catccaaaaa cctgggaggt atcggtgacg
    11701 gcggcatggt cgttacaaac gatgctgacc tgtatgaaaa aatgaagatc ctgcgcgtcc
    11761 acggatcgag ccccaaatac tatcacagag ccgtcggagg aaatttcagg ctggatgaga
    11821 tacaggccgc tgttttgctg gtgaagctga aatatctgga tacgtggacg gaagccaggc
    11881 agaaaaacgc gcgcatatat gatgagcttt tatccggcgt ggtttcggaa catttccgga
    11941 caccggccgt ccttcccgga tacaggcaca tatacaacca gtactgcatc aggacggcga
    12001 aacgagatga tctgaaagtt catctgagag aaaacggtgt tgcgacagag atatactatc
    12061 cgcttccgat gcacctgcag gaatgcttcc gttatctcgg atataagaaa ggcgactttc
    12121 ctgaggcgga agaagcggcg cttacatcgc ttgcgctgcc ggtttatccg gagatgggcg
    12181 cggacgggct ggaatatacg gctgatctga tcaggaactt tatcatgcgg taaagggccc
    12241 gggctctccg gacctgttat ggattgccga tgttttcata ttcatcgatc tgctcaaggg
    12301 tgacccgcat catatctgcc ttgtccagga aggcggtcgt ccagtatgcg gtcttctcca
    12361 gcgctttttc tatatcgagt ctcggtctga atccgagcaa ttgcttcgcc tttgcagaat
    12421 cgagcatcag gcagtttgtt tcatgcgggt tgttttccgt atcggccacg tatccgtatt
    12481 ctcttccaaa gtaatcaagg agccttccga ctatccactc cacagttttt atacttcctt
    12541 ggtcaggccc gaaattccag gcgccggaat aggccggtcc atcagtgtac agcttctgcg
    12601 ccagggtcat gtatccgaaa agaggttcca tgacatgctg ccatggcctg acagcccggg
    12661 gataccttat gcggagctgt tccccgctga tgatgcttct tattatgtcc ggcacgagcc
    12721 tgtcctgagc aaagtctcct ccgccgatca cgttcccggc acgtgcggtg gctatagcca
    12781 cgccatgcct gctgtattcg gcagtattaa agaaagagtt gatgtaggcc gaagttacaa
    12841 gttcctggca ggctttgctg ctggaatacg gatcgtatcc cccgagcctg tcggtttccc
    12901 tgtaaccccg ggaagattcg gtattttcgt aacatttgtc ggatgtaacg actacaacgg
    12961 ccttgacgga agggcagcgt cttatcgcat caagcaggtt caccgtaccc atgacgttgg
    13021 tctcatatgt ctcagccggc ctgtcatatg aaacccttac cagcggttgt gcggccaggg
    13081 gatactcgac gcgctgcagg cccggcgaca cg
//
LOCUS       DAOSCC010000038        13073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3222_length_13073_cov_4.623752, whole genome shotgun sequence.
ACCESSION   DAOSCC010000038 DAOSCC010000000
VERSION     DAOSCC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_13073_cov_4.623752"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..2131
                     /locus_tag="PLG72_03485"
     CDS             170..2131
                     /locus_tag="PLG72_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ79892.1"
                     /translation="MESFRRRILIADDNKDIHDDIKYILDTSSSGMEEYQEAKLLKKE
                     LFGDRDAASDQSETVIDIRYSIDDAYQGNEAVEMVRTAKESGDPYSLVFMDVRMPPGM
                     DGIETIESIWKIDPDVGVVICTAYSDYSWNQIVTKLGQNDNLLFIRKPFDHVSLKQIA
                     LAMTTKWSLKMQIREHIENLENLVAQRTNELTETVGRLREEISLREEKEKQLAYSAHY
                     DSLTDLLNRRSFYTSLAALTGNGEDRRDSFSLFYIDLDNFKNVNDLYGHDVGDRLLVE
                     VGRRISSGLEGHACMIRDYVSKDGKVKAVFRLGGDEFTAVIEETDRRTVGEIAQKLID
                     MIKAPYFIASHELDISCSVGICVYTSDSTQADMLLKYADIALYEAKKIHGIYRFHELS
                     SSYMHLNEIRIESELKKAIERNQIDVYLQSLVDTAERIVGVQALVRWLHPEYGELEPG
                     QFLHIAQKSDMIITLGHYVLRKALMYLKKIHESGHDDLFIMISCTAREFYHPHFVSNI
                     KSALDETGIAPEHLKLNLEDKFSFQAAQNALAIIRELSEMGVQFAVSGFETAYPAFAF
                     LQQMPKDTMIKLNKAYVQNIVADKRNRNFLLSLMDIISTWDLEVIISGIETKEQKQLL
                     DSRRCILQGYHFNMPKPFEDYLKDLGQGV"
     gene            2133..4694
                     /locus_tag="PLG72_03490"
     CDS             2133..4694
                     /locus_tag="PLG72_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ79893.1"
                     /translation="MLQYYYLDSGPVLKDRYTIGFLDDNIENDYHNLILQGISEAARE
                     LGVQIIRFGYYSSYIAYKFSHQVDMILDYIKQYDLDGLMFIGWTQAGAMYNREDFMER
                     FKNTPLLSIGTIFSDMPSVYFPGDMAVYKLTRHLIDEHHYQRIAYIEHFREDNRKEAY
                     ELAMNEAGLYDPSLYIGNDLLPPFTDLKGRIKRIADILVSIGARAVISMSYPYTFLLM
                     QELRERGISIPDEMAVVSYEEGEFAKYSVPGCTTAYFPWRELGYEGCKTLVRLLRTGS
                     CLKELPLDDKCMIIYRESCGCMPYYVRPVDAEYIRPAGCSPCAMTPSEKDAIIRALQA
                     LYSDSGILFGELVDAFIQSCDFRDSEIFLAELDSQLRSLGEIPGISRLVADIRYLFYP
                     YLISNIEMMLWSGDLFLQAQALVSRREAAVTGSAALKSKADDQILQDMTQQLLLSFSL
                     ENLLSTLERCMKLFRVDYCQIFISNSVLTGSDAEENLFDNSVLIFRYRDGMRKETTGK
                     ADSLKRQLAGIQAGELGRVSLAYLLHVTDEILGFALFGMRDGTPDEMILQSLTNSIST
                     ALHGIVLLKRLDIAYRKLVEHAQKEGMADIAANILHNIGNILNSINVSVHMMAECAVS
                     PVIDDIAMACELLKQNADRLEEFICTDGKGKKLMEFYLRLGGPAGKLRDQLLSILGRL
                     GAKTGTINEAIAAQQSYAGVDTKLEELLIAPIVDDALKLNEDSFEKLQITVEKDYMPG
                     LKAYIHRAKLFFVIFNIISNARDALVNSDAKDRKLAVRLYEDETGKYLCISDNGRGIP
                     KSLIDSIFEYGFSTKEGRYGYGLYSCAAYMADMGGSIRAESEGINKGASFILKFS"
     gene            4709..7237
                     /locus_tag="PLG72_03495"
     CDS             4709..7237
                     /locus_tag="PLG72_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ79894.1"
                     /translation="MEKKITIGFIDEDAYDEYHNMLTRGVHEYAREHGINVIRFGHFL
                     VHSTLRSLYHEKMLLDQIKQFRLDGLVFLGWARVTHNSEFLSMFSDIPLVSVGSTRPG
                     IPGVFFKGEYYLSQILHHLVQKHNYRKIAYISPFMPDSRNEMYLSIMSEYNIFDPRLV
                     VDRTELFGLDVNERGKRAVEILLDERKVIPQAIVSLYNEETLGVVSAIRDRGLRVPED
                     IAVTSYEDGEIGRFSSPAYTTVYFPWKELGYYACETLHRLIRGEGVPMQAEVPGKVVY
                     RESCGCVPRLAVSLDADKRNITGRTFEELDDRELDDIAGKISGITPFTPEELSEILGS
                     FRQAFCKMEYRPFLMKFEKMLRRIVYSDEPSDYEAAAADFRKVLMPYFLPYFRTDIKK
                     MIWADNIFNQMQGVLQNKLGTAIFMDHVEYNRIQLILREVGKILLTNFNLDTLKESLA
                     SNLPRIGVKGCWLYLFDEPSEDMEFSDYHPEFVYYDGEQIRDCTRHERSESGCLTDGI
                     FPDGDTHFLLSHLLYMGDEFMGFVLIETDHTDIRNMRVLSNYISLALKGIILFERLDR
                     SYRKLMEQAHRKGMADTTGILHNIANIMNSVNTTQQALESLMENSCLDDLLMANEMLS
                     KRIDDLESFVKNEEKGKLLMKFYAALGDSFIKFREDLKGYVDRLLERVSLIEALINTQ
                     QSLTGVKSSLERLDVIQVIENVLKLNQGSIEKAGIKIARRYNKSVRALAQSTKLFHVL
                     TNVVKNAIESMEGVEDREKVLTIEVTRDNGHIFIRVSDTGPGIEEGKLESIFAYGFTT
                     KQSGHGFGLHSCANYMTEMKGRIWAENAKEGKGAVFVMQFRAPS"
     gene            complement(7307..8833)
                     /gene="spoVB"
                     /locus_tag="PLG72_03500"
     CDS             complement(7307..8833)
                     /gene="spoVB"
                     /locus_tag="PLG72_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HOJ79895.1"
                     /translation="MKTRPWDHIDKFYRDSFILTLSNMTTGTIAFAFSILLSRKLGAE
                     GLGLYGLVMPVYGLLLCVTSDGIITAISKISTVYFNRREYKNLDRTLSSIFVLTALWA
                     CAVAMLVIIFHRPLAVYVVRDARASGALMVLSPALVFVPLSAIIKGYFYGMGKYRITA
                     AIDILEKLLRVGVLLTSIALLEPKSVSGTVAAAFLALTAGELVSFIALNTCYRIKKSR
                     LKGGTGRIKSRIQLVFDVMVISVPLCFNGIVSSVISTVSALILPRRLMAAGFTYRDAL
                     ALIGRFGGMALNISTLPFIIISSMLTVLVPDVSLNISKKDYWSAEERIAQVMRISCVI
                     GIATSIVCFVIPGTLGRVFYQRNDLTEMIRFSAPICLMTFISSPTFGVLNALGKQNIL
                     LRNSLINSLQNLVLTAVLAGIPALNIYGYGLSVIITSATSLILNTREIRKICEIKIGL
                     QDIAIMVFSGTVSWLAALLAGNALLAAPPPARMAGVVIACFGTMAAITSYAFKLRAES
                     "
     gene            complement(8890..9648)
                     /locus_tag="PLG72_03505"
     CDS             complement(8890..9648)
                     /locus_tag="PLG72_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine gamma-glutamyltransferase"
                     /protein_id="HOJ79896.1"
                     /translation="MIRFEDGFTDLPSIMARYRRGSPEYEVLSKLSESSRVYTYLSPE
                     ELEFEIGLRRGIIEASLALFRGRLAFRIFQESFCNERYWERRQDGGFVLRAGVRPSEA
                     VDDIFANTRRYGTECATAIVMIFYKAVLDAYKPDLFDRTFTRIVLMNWRDMDPLIDPA
                     TYRGLADYIPGDCRYIRNPDVDPLTPEWQGENVIDLGGGRYYGHGIGLGDMDFFIEIL
                     NRNRREGAQVSAYLVDSATRPDFRGLYLYWKNAS"
     gene            complement(9645..10694)
                     /locus_tag="PLG72_03510"
     CDS             complement(9645..10694)
                     /locus_tag="PLG72_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79897.1"
                     /translation="MTGSPVSLLDNIRISDGPEILRNYFSELLHTSRNRLPELLSDRR
                     LHFGTLYLLKSEIKSSGLQDTLPDMYRRALELSEELSGTGPGDTRPAGRRPGARPSYG
                     HRAGYKGRNIGVMLSDAVTVRSLGWIVRTGWDSDMPEGPYECLMERCTSLLLAHMRDK
                     SFLPLIADKIFERNRQGRLIHNLVWAFFEARDRESLYFLAHRLCSPDMRDAMLARKLL
                     GFIPGTDDYSADSHVLYINALGWLSVNMPFMYYTGESLQMSPKPVFYAVSYEAKYLCR
                     KVPADSGRPAVPPDKHEAGRLECFRRLDETQRQLLADVSYALFMQDKRRWESWIRLPV
                     RDQLESAVHMTGGSA"
     gene            complement(10741..11694)
                     /locus_tag="PLG72_03515"
     CDS             complement(10741..11694)
                     /locus_tag="PLG72_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79898.1"
                     /translation="MHPPKGSGLEALDRMVRRSVKFYSDLFDTDRFRRNMLSALKKLP
                     TVELPTLADLRKPVDNLVKTLNNAVSQFTYKPPKFDYRNIVNGFLPPGARLISPRYPE
                     GAGEIRFADLDGDGRSELVTSYTAADGLRTLVLKKDEVQWYKMAEISSPGFSSIHYLT
                     CADISGEGKKDLLLGLDSEQNSRTLFAYSVSDGGSRKIFSKKYNKLEVIRSSSRTSRD
                     AVALWHEDSPGIYRIELIRWNGIDLEQIDKTRYLAAKVLPYYIRKLRQDPSDITGWYN
                     LAGTFMEAGDRANAAKAVRYTLEFNPDPVMKDRLMELRQKL"
     gene            11857..12225
                     /locus_tag="PLG72_03520"
     CDS             11857..12225
                     /locus_tag="PLG72_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79899.1"
                     /translation="MSKSRPTILICDDSLLVRKKMRDCLNEYGEFDIIEASSGQSSIE
                     LFKQHDPDLVFLDIVMPDKNGIDALKEMMAVKKDVKVVMLSSSGTRSHLKAAMEAGAH
                     DFIQKPWEKSQIEHIIRNIF"
     gene            12241..>13073
                     /locus_tag="PLG72_03525"
     CDS             12241..>13073
                     /locus_tag="PLG72_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOJ79900.1"
                     /translation="MFSQYFGNYLLNRGIIKPEQLTDALEYQRSVHLKLGVIAVNAGY
                     MTPAQVEEIHNMQKKADKRFGELAIEKGYITGQQLEEMLATQRQGHLMLGQALIDRGH
                     LTIEQLQKALDEYKNEYGMSTRQFNVVRKDDSQEIEQIFRDYGEALLGKMYSDYVTLL
                     MKNLIRFVDDNPTIEVWTALSFMAARWPLFKFGPMVLGQSYRNPALMAKMAATLQILT
                     GGRLIFGLGAGWKEDEYHAYNWPFPSVGTRLQQLDEALEIIKRLWTQSGPVTWEGKHY
                     K"
BASE COUNT         3429 a         3172 c         3480 g         2992 t
ORIGIN      
        1 aattgaaata atttgtcgca gatattataa tggaaatact ggtacataat tgacgaccaa
       61 tcgacatcag caaagcaaat tcagataaat ttcgcacctg tgcacaagcc gtgtcgaaaa
      121 ttcttataaa gccgtcagag aataaacttg caccaaagtg aggattatta tggagagctt
      181 tcggagaagg atactgatag cagacgacaa caaggatatc cacgacgaca ttaagtatat
      241 acttgatacg tcttcctccg ggatggagga gtatcaggaa gcgaaactgc tgaagaagga
      301 attgttcggg gacagggacg cagcgtcaga tcagagcgaa acagtaatag acatcaggta
      361 ttccatcgac gatgcatatc agggcaatga agctgtcgag atggtaagga cggccaagga
      421 atccggcgac ccgtattcgc ttgtgttcat ggatgtcagg atgccgcccg gtatggatgg
      481 tatagagacg atcgaaagca tctggaagat cgaccctgac gtcggtgtcg tcatatgcac
      541 cgcatattct gattattcgt ggaaccagat cgttacaaag cttggccaga acgacaatct
      601 cctgttcata agaaaacctt tcgaccatgt gtccctcaag cagatcgcgc ttgcgatgac
      661 aacgaagtgg agcctgaaga tgcagatcag ggagcacatc gagaaccttg agaatctggt
      721 ggcgcagagg accaacgagc tcaccgaaac agtgggcagg ctcagggagg agatatctct
      781 cagggaggaa aaggagaagc agcttgccta ctccgcccat tatgattcac ttacggacct
      841 tttgaacagg cgttccttct acacttccct ggcggcactt accgggaacg gcgaggaccg
      901 cagggacagt ttctcgttgt tttacatcga tctggataat ttcaagaacg taaatgattt
      961 gtacggacat gatgtgggcg acaggctgct ggtcgaggtg ggcagaagga tcagcagcgg
     1021 gctcgaaggc catgcatgca tgatccggga ttatgtgagc aaagacggca aagtcaaggc
     1081 tgtattcagg ctcggaggcg atgaattcac agccgttata gaagagacag atcgtaggac
     1141 ggtcggagag atcgctcaga aacttataga catgataaaa gcgccgtatt tcattgccag
     1201 ccatgaactg gatatttcct gcagcgtagg gatctgcgta tacacgagcg acagcacgca
     1261 ggccgacatg ctgctaaagt atgcggatat agcgttatac gaagcaaaaa agatccatgg
     1321 catttatagg ttccatgaac tttcgagttc atacatgcac cttaatgaaa tcaggataga
     1381 gagcgaactg aagaaagcga tcgaaaggaa ccagatagac gtatacctgc agagcctggt
     1441 cgatacggcc gaaagaatag taggcgtgca ggcgctcgta cgctggctcc atccggaata
     1501 cggagaactg gagcccggcc agttcctgca tatcgcgcag aaatccgata tgatcataac
     1561 gcttggacac tatgtcctgc gcaaagctct catgtacctc aaaaagatcc atgaatccgg
     1621 acacgacgac cttttcataa tgatcagctg cactgccagg gagttctatc atccgcactt
     1681 tgtcagcaat ataaagtctg ccctggacga aaccgggatc gcgccagagc atctcaaact
     1741 caacctggag gataagttct cgttccaggc tgcccagaac gcgcttgcca tcatcaggga
     1801 actgagtgaa atgggggtcc agttcgctgt tagcggattc gagactgctt atcctgcgtt
     1861 cgcgttcctg cagcagatgc caaaggacac gatgatcaag ctgaacaagg cttatgtgca
     1921 gaacatcgta gctgacaaaa ggaaccgcaa cttcctgctc tcgctcatgg atatcatcag
     1981 cacgtgggat cttgaagtga taattagcgg tatagagacc aaagaacaga aacagcttct
     2041 cgattcaaga cggtgtatcc tgcagggtta tcatttcaac atgcctaaac cgtttgagga
     2101 ttatttgaag gacctggggc aaggagtatg atatgctgca atattattac ctggattccg
     2161 gacctgtcct gaaggaccgt tacactatcg gctttctgga cgacaatatc gagaacgatt
     2221 accacaacct gatactgcaa gggatatccg aagcagccag ggaactggga gtacaaatca
     2281 tcaggttcgg ttattactcc tcttatatag cctacaaatt ctcccaccag gtggacatga
     2341 tcctggacta cataaagcag tatgacctgg acgggctgat gttcatcgga tggacgcagg
     2401 ccggcgcgat gtataaccgg gaagatttca tggaacgatt caaaaacacg ccgctgctca
     2461 gtatagggac tattttcagc gacatgccaa gcgtgtattt tcccggtgat atggctgttt
     2521 acaaactgac ccggcacctg atagatgaac accattatca aaggatagcc tatatagagc
     2581 atttcaggga agacaacagg aaggaggcat atgaactggc aatgaatgaa gccggtttgt
     2641 atgatccttc cctttatatc ggaaatgatc tccttccccc cttcacggac ctgaaaggca
     2701 ggataaaaag aatagctgac atactggtca gtatcggggc cagggcagtc atatccatgt
     2761 catatccgta tacctttctt ctcatgcagg aactcaggga gagggggatc agcataccgg
     2821 atgaaatggc tgttgtcagt tacgaggaag gcgaattcgc aaaatactct gtccccggat
     2881 gcaccaccgc atactttccg tggcgggaac tgggttatga gggatgcaaa accctggtca
     2941 ggcttctgag gacgggcagc tgcctgaaag aattgcccct ggacgacaag tgcatgatca
     3001 tatacaggga gtcctgcgga tgcatgcctt attatgtcag gcctgttgat gccgaatata
     3061 tcagacctgc cgggtgttcc ccgtgcgcca tgacgccttc tgaaaaggat gcgataatac
     3121 gggcattaca ggctttatac agtgattcgg gcattttgtt cggggaactc gtggatgcgt
     3181 tcatacagtc ctgcgatttc agggacagcg agatattcct ggcggaactg gattcacagc
     3241 tccggtccct gggggagatc cccggaataa gcaggctggt ggctgatatc aggtatctct
     3301 tctatccgta tctcatttcg aacatcgaga tgatgctgtg gtcgggcgac ctgttcctcc
     3361 aggcacaggc gctggtcagc agaagggagg cggcggtcac cggcagtgcc gctcttaaaa
     3421 gcaaagcaga cgatcagata ctccaggaca tgacacagca gctgcttttg agtttttccc
     3481 ttgagaatct gctcagtacc cttgaaaggt gcatgaaact gttccgcgtg gactattgcc
     3541 agatattcat ttcaaactcc gttttgaccg gctcggatgc agaggagaac ctgtttgaca
     3601 acagcgtgtt gatcttcaga taccgtgacg ggatgaggaa agagaccacc ggcaaggccg
     3661 acagcctgaa aaggcagctt gccggaatac aggccggtga actcgggagg gtatcgctgg
     3721 catatctgct gcatgtgacc gatgagatcc ttgggttcgc gctgtttggc atgagagacg
     3781 gaacgcctga cgaaatgatc ctgcaaagcc tgacaaacag catcagtacg gcactgcacg
     3841 gtatcgtcct gctcaaaagg ctcgatatcg catacagaaa actggtggaa catgcgcaga
     3901 aggaaggcat ggctgatatc gcagcaaata tcctgcacaa tataggcaac atactcaaca
     3961 gtataaatgt ttccgtgcac atgatggcag aatgcgctgt ttcgcctgtc atcgacgata
     4021 tagccatggc atgcgaactg ctgaaacaga acgccgacag gctcgaggaa ttcatatgta
     4081 ccgatgggaa aggcaaaaag ctgatggagt tttacctgcg cctcggcgga ccggccggga
     4141 agctgcgtga ccagcttctc agcatccttg gcaggctcgg ggcgaagaca ggtaccatca
     4201 acgaagcgat cgctgcccag cagagttacg ccggcgtgga tacaaagctg gaagagcttc
     4261 tgatagcgcc gatagtcgat gacgcgctca aactcaatga agattctttc gaaaaactgc
     4321 agataacagt ggaaaaggat tatatgcctg gtttaaaggc gtacatccac cgggcgaagc
     4381 tgtttttcgt cattttcaat atcatttcga acgcaaggga cgcgcttgtc aattcggatg
     4441 ccaaagacag aaaacttgcc gtaaggctgt atgaagacga gaccggcaag tacctgtgca
     4501 taagtgacaa tgggaggggg atccccaaat ccctgataga cagcattttc gaatacgggt
     4561 tttcgacaaa ggaaggccgg tacggctatg gcctatatag ctgcgctgca tatatggccg
     4621 acatgggcgg atcgatccgg gctgaaagcg aaggcataaa caagggagcg tcttttatcc
     4681 tgaaattttc atgacaggtg gaagctgtat ggagaaaaag atcaccatcg gttttattga
     4741 cgaggatgct tatgacgaat accacaacat gcttacaagg ggcgtacacg agtatgcgcg
     4801 ggagcatggc ataaacgtga ttcgtttcgg gcatttcctt gtccattcga ccttaaggag
     4861 cctgtatcat gagaaaatgc tgcttgacca gataaaacag ttcaggctgg acgggcttgt
     4921 tttccttggt tgggcacgcg taacgcacaa cagcgaattt ctcagcatgt tcagcgatat
     4981 cccgctggtc agcgtaggat cgacaagacc cggtatcccc ggtgttttct tcaaaggtga
     5041 atattacctg agccaaatac tgcatcatct cgtacagaag cacaattacc ggaagatagc
     5101 ctatatctcg ccatttatgc ctgacagccg gaacgaaatg tatcttagta taatgagcga
     5161 atacaatatc tttgatcccc ggcttgtcgt ggaccggacg gaacttttcg ggcttgatgt
     5221 gaatgaaaga gggaaaagag ccgtagagat cctccttgat gaaagaaagg ttattcccca
     5281 ggctatcgta tctctttaca atgaagagac cctgggcgtg gtcagtgcga tcagggacag
     5341 gggcctgagg gtacctgaag atatcgctgt gaccagctat gaggacggag agatcggaag
     5401 gttttcatcg ccggcataca ccacggtcta ctttccgtgg aaggagctgg gatattatgc
     5461 gtgcgaaact ctgcacaggc ttatcagggg cgaaggcgtc ccgatgcagg ctgaagtccc
     5521 aggaaaggta gtgtacaggg aatcgtgcgg ctgcgttccg agactggcgg tttccctgga
     5581 cgcagataaa aggaacatta ccggccggac ttttgaagaa ctggatgacc gcgaactgga
     5641 tgacatcgcc gggaaaatct ccggtatcac gcccttcacg cctgaggaac tgagtgagat
     5701 attgggttcg ttcaggcagg cattctgcaa aatggagtat cgcccgttcc tgatgaagtt
     5761 tgagaaaatg ctccgcagga tagtttacag cgatgaaccc agcgattatg aagcggcggc
     5821 cgctgacttc aggaaggtgc tcatgcctta tttcctgccc tatttcagaa ccgatataaa
     5881 gaaaatgata tgggccgaca acatcttcaa ccagatgcag ggcgttttgc agaacaagct
     5941 gggcaccgcc atattcatgg atcatgtgga gtacaacagg atccagctca tattgaggga
     6001 agtcgggaaa atattgctta cgaatttcaa cctggatacc ctgaaggagt cactggcatc
     6061 gaatctcccg aggatcggag tgaaaggctg ctggctttat ctgttcgatg aaccttcgga
     6121 ggatatggag ttttcagatt accatcccga atttgtatac tatgacggcg aacagatcag
     6181 ggattgcacg cgccatgaac ggagcgaatc cgggtgcctg accgacggca ttttccccga
     6241 tggcgatacg cactttctcc tgtcccatct gctgtatatg ggcgatgagt tcatgggatt
     6301 cgtgcttatc gagacggacc atacagacat aaggaacatg cgtgtactca gcaactatat
     6361 cagtcttgca ctgaaaggca taatactgtt cgagaggctc gaccgaagct acaggaaact
     6421 gatggaacag gcacatcgga agggtatggc tgatactacc gggatactgc acaatatagc
     6481 caatataatg aacagcgtga acactacgca gcaggcgctt gaaagcctga tggaaaacag
     6541 ctgcctggat gacctgctga tggcgaacga aatgctctca aaaaggatcg atgatcttga
     6601 gtccttcgta aagaatgagg aaaaaggaaa actcctgatg aagttttatg ctgccctggg
     6661 cgattcattc atcaagttca gggaggatct taaaggatat gttgacaggc tgcttgagcg
     6721 tgtcagcctc atcgaggcgc tcatcaacac tcagcaaagc ctgacaggcg ttaaatccag
     6781 ccttgaacgc ctggatgtga tacaggtcat tgaaaatgta ctgaaactaa accagggatc
     6841 tatagaaaag gcaggaataa aaatcgccag acgttataat aagtcggtcc gggcccttgc
     6901 acaaagcaca aaactcttcc acgtgcttac gaatgtggtg aaaaacgcaa ttgaatcgat
     6961 ggaaggcgta gaggacaggg aaaaagtcct taccatcgag gttaccaggg acaatgggca
     7021 tatctttatc cgcgtcagtg atacgggacc ggggatagag gaaggaaaac tggaaagcat
     7081 ctttgcctat ggctttacga ccaagcagag cggacatggc tttggccttc acagctgcgc
     7141 caattacatg acggagatga aaggcaggat atgggctgag aacgcaaagg aaggaaaagg
     7201 agccgtgttc gtgatgcagt tcagggctcc gtcctgatgc ggtccgggtt tccgtcaaaa
     7261 tgcagtccgg ttgtcatgac gcagttcgtt ttcgtcatga tacggttcag ctttcagccc
     7321 tgagcttaaa agcgtaagaa gtgatagccg ccattgtacc gaaacaggct atgaccacac
     7381 ccgccatccg cgcaggaggc ggcgccgcca gcagcgcatt ccccgccaga agggccgcaa
     7441 gccaggatac cgtaccggaa aagaccatta ttgcgatatc ctgcagacct atctttatct
     7501 cgcagatttt tctgatctcc ctggtattca atatgagcga cgtggcagaa gtaatgatga
     7561 ccgagaggcc gtaaccgtaa atgttgagcg ccggtatgcc ggcaagcacg gctgtgagca
     7621 caaggttctg cagggaattg atcagcgagt tccgaaggag gatgttctgt tttccaaggg
     7681 cattgagcac gccaaaagtc ggagatgata tgaaagtcat caggcagatg ggcgccgaga
     7741 accggatcat ctccgtgagg tcgttccgct ggtagaaaac ccgcccaagc gtcccgggta
     7801 tgacgaaaca gacgatggaa gtcgctatgc cgatgacgca ggagatcctc atgacctggg
     7861 cgatcctttc ctcagcggac cagtaatcct ttttgctgat attgagcgac acgtccggca
     7921 ccagtacagt gagcatggaa cttatgatta tgaaaggaag cgttgatata ttgagcgcca
     7981 tcccgccgaa cctgccaatc agcgccaggg cgtccctgta cgtgaatccc gccgccatca
     8041 gcctgcgggg cagtatcagc gctgaaacgg tcgatatcac cgacgaaacg ataccgttga
     8101 aacagagagg gacggatatg accattacgt cgaaaaccag ctgtatcctt gatttgatcc
     8161 tgccggtgcc gcccttgagc ctgctctttt ttatcctgta gcaggtattg agggcaataa
     8221 agctgaccaa ttctccggcc gtcagggcaa gaaaggccgc tgcgaccgtg ccggatacgc
     8281 ttttgggctc cagcagcgcg atggatgtca ataaaacgcc taccctgaga agtttttcga
     8341 gtatatctat tgcggctgtt atcctgtatt tgcccatacc ataaaaatag ccctttataa
     8401 tggctgaaag cggtacgaag acaagcgcgg gagacagcac catcaaagct cccgatgccc
     8461 gggcgtccct cacgacatat accgccagcg gcctgtggaa tataatcacc agcatggcga
     8521 cggcgcaggc ccacaaagcg gtcagcacga atattgaact gagcgtcctg tccaggtttt
     8581 tatattccct gcggttgaag tagacggtag aaattttgga tatggctgtt atgatgccgt
     8641 cgctggtgac acacagcaaa aggccgtaga caggcattac cagcccgtac agtccaaggc
     8701 cttccgcgcc cagtttcctg gaaagcagga tggaaaaggc gaaggcgatg gtgccggtgg
     8761 tcatgtttga gagcgtcagt atgaaagaat cccgataaaa cttgtctata tggtcccagg
     8821 gccgggtctt caaaatatgt acaccaaata accttttcat aagtaaaagc tattcattgg
     8881 cgatgttttt tatgaagcat ttttccagta caggtacaaa ccccggaaat caggccttgt
     8941 agccgaatca acgagataag cggacacctg ggccccctct ctcctgttgc ggttcagtat
     9001 ctcaatgaag aaatccatgt cgcccaaccc tataccgtgc ccgtaatacc tgccgccgcc
     9061 aaggtctatg acattttcgc cctgccactc cggcgtcagc ggatccacgt cggggttcct
     9121 gatatatctg cagtcgcctg gtatataatc agcgagcccg cggtatgtcg caggatcgat
     9181 aagcgggtcc atatcccgcc agttcatgag aacgatcctc gtgaatgtgc ggtcgaacag
     9241 gtcaggttta tatgcgtcca ggacagcttt gtaaaatatc attactatgg ctgtggcgca
     9301 ttcggtcccg tacctgcggg tattggcgaa aatatcgtcc accgcttcgg aaggcctgac
     9361 accggcccta agcacaaaac cgccgtcctg cctcctttcc cagtagcgct cgttgcagaa
     9421 ggactcctgg aatatccgaa atgccagcct ccctctgaac agggcaagag aggcttcgat
     9481 tatgcctctt ctcaggccga tttcaaattc cagttcttcc ggggacaaat aggtataaac
     9541 gcggctgctt tccgaaagct tgctcaaaac ttcgtattcc ggactcccac gcctgtaccg
     9601 ggccattatg gatggaaggt cggtaaaacc gtcttcgaac ctgatcatgc tgatcctcct
     9661 gtcatatgga ctgcggattc gagctggtct ctgacgggaa gtctgatcca ggattcccat
     9721 cggcgcttat cctgcatgaa aagcgcatag gatacatctg ccagcaattg cctctgagtt
     9781 tcatcaagcc tgcggaaaca ctcgagacgt ccggcttcat gcttgtcagg cggcacggcc
     9841 ggtctgccgc tgtctgcagg aaccttacgg cacaggtact tcgcttcgta tgaaacggca
     9901 taaaagaccg gcttcggact catttgcagg ctttcgccgg tatagtacat aaaaggcatg
     9961 ttaacggaca gccagcccag cgcattgatg tacaggacat ggctgtcggc gctgtaatca
    10021 tccgttccgg gaatgaagcc gagcagtttc cttgccagca tcgcatcacg catgtccggt
    10081 gaacacagcc tgtgggcgag gaagtagaga ctttcccggt cgcgtgcttc gaaaaatgcc
    10141 cagaccaggt tatggatcag cctgccctgc ctgttgcgtt cgaaaatctt atcggctatc
    10201 agcggcagga acgacttgtc tctcatgtgt gccaggagca atgacgtaca gcgttccatc
    10261 aggcattcat agggaccttc cggcatatcg ctgtcccatc ctgttctgac gatccagcca
    10321 agcgaccgga cagtgaccgc atcagagagc atcacgccaa tgttccggcc tttgtatcct
    10381 gcccggtgtc catacgacgg ccgtgcgcct ggacgtcttc cggccggcct ggtatcgccg
    10441 ggtcctgtgc ctgataactc ttcggacagt tcaagagccc ttctgtacat atctggcaat
    10501 gtatcctgaa ggccgctgct tttgatttcg gatttcagca gatacagcgt gccgaaatgg
    10561 agacggcggt cgctgagcag ttcaggaagc ctgttcctgc ttgtatgcag cagttctgag
    10621 aaatagttcc tcaggatttc cggaccgtcg ctgatcctta tattgtcgag cagggaaacc
    10681 gggctgcctg tcatatgcgt ctgcctcctt cacggttgtg tcatccggcg gaccgaactg
    10741 tcaaagcttt tgccgaagtt ccatcagccg gtctttcata accgggtcag ggttgaattc
    10801 gagcgtgtat cttaccgctt tggcggcatt tgccctgtcg ccggcttcca tgaatgttcc
    10861 ggccaggttg taccaaccgg tgatgtcgga gggatcctgc ctgagtttcc tgatataata
    10921 gggcaatacc tttgcagcca ggtatcttgt tttatcgatc tgttcgagat cgatgccgtt
    10981 ccatcttatg agttcgatcc tgtatatgcc cggtgaatct tcatgccaca atgccacggc
    11041 gtctctcgaa gtacgtgagg aactcctgat cacttccagc ttgttgtatt tcttgctgaa
    11101 tattttcctg gagcctccgt ccgacaccga atacgcgaac agcgtccggc tgttctgttc
    11161 cgagtcaagg ccgagaagga gatccttttt gccttctccg gagatatcgg cgcatgtgag
    11221 gtagtggatg ctgctgaaac cggggctgct gatttcggcc attttatacc actgcacctc
    11281 gtcttttttc agtacgagcg tcctcaggcc gtcggccgcg gtatacgatg ttacgagttc
    11341 gctgcgcccg tcgccgtcaa gatcggcaaa ccggatctct cctgcgcctt ccggataccg
    11401 ggggcttatc agccttgcgc cgggcggcag gaaaccgttt acgatgttcc ggtaatcgaa
    11461 tttcggaggt ttgtatgtga actgcgaaac agcgttgttg agagttttta caaggttgtc
    11521 gaccggcttt ctcagatcgg ccagggtcgg cagttcaaca gtcgggagtt ttttcaatgc
    11581 tgacagcatg tttcgtctga accgatccgt atcgaaaaga tcggagtaaa atttgacgct
    11641 tctccggacc atcctgtcca gtgcttcaag tcccgagcct tttggaggat gcatgtggaa
    11701 gactccttta caatatatac tgcttctata tactatgcag aagtgaacaa aacgttaaaa
    11761 catatcaaat ataagcattc ctttacatat cgggaaaatt aagatattat aataatataa
    11821 caaaattcta cattttttct cggggagggt attgtaatga gcaagagccg gcctacaatt
    11881 ctcatctgcg acgattctct gctggtcagg aaaaaaatga gggattgcct taatgaatac
    11941 ggtgaatttg acataattga ggcatcaagc gggcaaagct ccatcgaact gttcaaacag
    12001 cacgatcccg accttgtctt cctggatatc gtgatgcctg ataagaacgg gatagatgcc
    12061 ctgaaagaga tgatggccgt gaagaaggac gtaaaggttg taatgctgtc atcttcggga
    12121 acaaggtcgc atctgaaggc ggcaatggaa gccggtgccc acgattttat acagaaaccg
    12181 tgggagaagt cccagatcga gcatatcatc aggaacattt tctgagaagg ggtgttgaat
    12241 atgtttagcc agtatttcgg caattacctg ctgaacaggg ggattatcaa acccgagcaa
    12301 ctgacagatg ctctcgaata ccaaagatcc gtacatctca agcttggcgt gatagccgtc
    12361 aatgcgggct atatgacgcc cgcgcaggtc gaagagatcc acaatatgca gaagaaagca
    12421 gacaagagat tcggagaact ggcgatagaa aaaggataca taaccgggca gcagcttgaa
    12481 gaaatgctgg ccacgcagag acaggggcat ctgatgcttg ggcaggccct gatcgacagg
    12541 ggacacctga ccatcgaaca gcttcagaag gcgcttgacg agtacaagaa tgaatacggc
    12601 atgtccacca ggcagttcaa tgtggtcagg aaggatgatt cacaggagat agagcagata
    12661 ttccgggatt atggcgaagc ccttcttgga aaaatgtaca gcgactatgt cacattgctc
    12721 atgaaaaacc tgatccgttt cgtggacgac aacccgacca tcgaggtttg gaccgccctg
    12781 tccttcatgg cagctcgctg gccgctattc aagttcggcc cgatggtcct cggccagagc
    12841 taccgcaacc cggccctgat ggccaagatg gccgccaccc tccaaatcct caccggcgga
    12901 cggctgatct tcggactggg cgcgggctgg aaggaggacg aataccacgc ctataactgg
    12961 cccttcccct cggtcggcac ccgcttgcag cagctcgatg aagccctcga gatcatcaaa
    13021 cgcctgtgga cccagtccgg cccggtgacc tgggaaggca agcactacaa gat
//
LOCUS       DAOSCC010000039        13072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3223_length_13072_cov_5.344473, whole genome shotgun sequence.
ACCESSION   DAOSCC010000039 DAOSCC010000000
VERSION     DAOSCC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13072
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3223_length_13072_cov_5.344473"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLG72_03530"
     CDS             <1..1147
                     /locus_tag="PLG72_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOJ79901.1"
                     /translation="MSSDFRGDPASAMLEVLDGEQNFAFRDHYLELPVDLSDVLFLTT
                     ANSLDTIPRPLLDRMEVITISGYTEEEKVQIAQRYLVNKQMKAHGLQKKNLKITEDAI
                     RCIINYYTREAGVRNLEREIANICRKAAKLLVSGSRKSVTVTPSNIEKYLGTRRYRFD
                     KANEKDEVGVATGLAWTPVGGDTLAIEVTVMDGSGKLELTGQLGDVMKESAMAAVSFI
                     RSRAELLGIDREFYKKYDIHIHVPEGATPKDGPSAGITLATAMVSALTGRAVKRNVAM
                     TGEITLRGRVLPIGGVKEKVLAAHRAGIDTVILPAENRKDIDEIPVNVRSGLKFVFVE
                     EMDTVLNTALTPEKNHRDIESGREDQEAVLLAEESAAVDGQSSTHRM"
     gene            1352..2641
                     /locus_tag="PLG72_03535"
     CDS             1352..2641
                     /locus_tag="PLG72_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79902.1"
                     /translation="MHDYMIAVICAVAVSGIIMLVVFHRLEVLSFRTVASITPITLAS
                     LITALIIPAIYSAISGGETDRADPTVIIMTTVAAFIAYVVIVLILSILISVIVPGVKP
                     RDKDGKTSKTNKKDVKSDGSMREKTILEQVGALYFNDRAGESREEQEGAVSGSGTEPV
                     AASIGSAEITAEAVHSETLPGMPEQPESGQDISYALDDQLYSAGMMPDHGTSNQLDTA
                     AASGTETEQYAASVANAGSGMDTAKVSEISSCTDGIQPEHEAVQTPEVQDFPDAKLHK
                     PLLAGDNYLEQIYLNYVAHGDELKEDAGEMTGEAASDAEKSVDSAEIIDKMGIENKVY
                     DSEAMTIEECITEAFRLKEAGDLEGAILYFMYALDKKPQKELTFWIILDICVMYKTLG
                     QQDLALEILNGYYEAYGDTMDDRLRDEIIRNLTDAGA"
     gene            2685..3239
                     /locus_tag="PLG72_03540"
     CDS             2685..3239
                     /locus_tag="PLG72_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOJ79903.1"
                     /translation="MKKTQKILGLPIISISDGMEVGKVKSIIINAEKGAIDYIAVDSG
                     IQIFSARVIATEDILGIGEYALTIENEDVITDISKIPSAIQLLQKDIRVKGTKVLTKK
                     GSLIGEIGDIYVDENNGCRITGLEFIADITQKQIRLIPRDSVITFGKNLTVVTEDVES
                     RLLDTPSQLDSDRGSLESEKKKYY"
     gene            3241..3624
                     /locus_tag="PLG72_03545"
     CDS             3241..3624
                     /locus_tag="PLG72_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79904.1"
                     /translation="MIGQFSAQDAEPEIKTGLDGGSAVDDLASALDEAAAIAGEETGS
                     EIAAGADVPSESADQPAEDDRDNASILFEQRQRQYLKGRYATKTITDNDGNVIIYEGM
                     QIDDAVIDEAKKKSRLVELVMNNRA"
     gene            3722..4936
                     /locus_tag="PLG72_03550"
     CDS             3722..4936
                     /locus_tag="PLG72_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ79905.1"
                     /translation="MKKTGNKLLIFLLILVQSLLAIGAGIAVLFNASGRVIPVNVYAG
                     DLDIGGKGFEEAARAIEADYEARFGVQHIRLKADDDQIYEIPFSSIEATVDGMATIEP
                     IMSMDGIEDIPRLIGTHFGNHTTVLSPVVKFNESKLRMKLIELSESINRDPVDARIYY
                     KDGLIEKEPDDPGVSLNVNNAAELIRRQLSADPFGVIDLRAGKAMDTVYADVTMKDLD
                     AIQVVLGEYSTSIMDGAVEDSIETAVEGINGIVLAPGGDPSGRSVFSFAETMLSLNPD
                     MTFDEGYCQVASTMYAALLEAGIPAENITRLPHKLTVDYIEPGLDAMVAEDAYDLRFS
                     NPFGNNLAIYAVKKGTVVTVAVAGSRDDRPDKSVISTQTVNKSVPPVYYVENRDLKPG
                     EQVVLDPGKESL"
     gene            complement(5046..5672)
                     /locus_tag="PLG72_03555"
     CDS             complement(5046..5672)
                     /locus_tag="PLG72_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HOJ79906.1"
                     /translation="MKIERIGDNIIRVTITYNDLEERNVDLESLNYNSPAAQEFFWDL
                     MEQAEEQLGFSLADSQLIIEPVPDSDEGFVINITRIDDEGEFESIHKYIKNRMKKSDL
                     RVKKKSRRVSSPFLIYSFKSMDDISELARRLEMHYSGESTLYRCGDSYYLLLTKSGLH
                     SYGMRMFELVLAEYGVKITNNSFMEGYLSEHGEKIIENDALEVLRRFY"
     gene            complement(6083..7153)
                     /locus_tag="PLG72_03560"
     CDS             complement(6083..7153)
                     /locus_tag="PLG72_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ79907.1"
                     /translation="MKKRITFLAVLLLAVAVTLSACGSPGSDNGNGTERVTLNVFNWG
                     DYIDESVIEDFEDKYPNIKIVYETYTTNEDMYVKIKTGSSNYDIAIPSDYMIKRMIDE
                     GLLKEINIDNIPNYKYIGDQFKNLAFDPENKYSVPYMWGTVGILYNKTMVTEPVDSWK
                     ILWDEKYKKQIIMSDSERDSIGITLKMLGYSMNSKSIDELEEAKQKLIEQKPLVLAYL
                     VDEAKDMMIAESAAMAVVWSGDALIAMRENENLAYAIPKEGSNYWFDSMVIPKTGKNQ
                     EAAELFINFLCDPEIALRNVNYIGYATPNVETMKLLDPEVITNSSAYPTEDQLVNCEI
                     FEYPGDEINRVYNRIWTEIKAG"
     gene            complement(7238..8032)
                     /locus_tag="PLG72_03565"
     CDS             complement(7238..8032)
                     /locus_tag="PLG72_03565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79908.1"
                     /translation="MKKAIKRIYLIIIFVFLYAPVLTLIVFSFNKSKSRGNWGGFTLK
                     WYGELFHDRQVLSSLYNTVIIALLSALVATVAGTAAAVGIHNMKKTVKSFVMNLTYLP
                     VVNPDIVTGISLMILFVFIKLDLGFTTLLLAHITFNIPYVVLSVLPKLKQLNKHMYEA
                     ALDLGAKPAYAFFRVILPEIMPGVVTGFLLAFTLSLDDFIISFFTTGSGVSTLSITIY
                     SMARLGISPKINALSTLMFIVVLVLLLIVNKRSSANGKNGAPIVNI"
     gene            complement(8029..8853)
                     /locus_tag="PLG72_03570"
     CDS             complement(8029..8853)
                     /locus_tag="PLG72_03570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79909.1"
                     /translation="MKKTWVSYPYILWMIIFTIVPLLLVLYYSFTRTENGTVVFTLDN
                     FIRVMEPTYLKVLWRSIVLAVISTVVCFILGYPVAMILAGKDISRKSTLVMLMVVPMW
                     MNFLLRTYAWMTLLDRKGVINSILGTLGLPQVHLLYTDFAVVLGMVYNFLPFMVLPIY
                     SVLIKIDRSLIEAAEDLGSDNLTVFRKVIFPLSLPGVASGVTMVFMPAVTTFIISKLL
                     GGSQYMLIGNLIEDQFMRVYDWNFGSAVSIIMMLLILLSMGVMSRNDKEGTGGALF"
     gene            complement(8850..9899)
                     /locus_tag="PLG72_03575"
     CDS             complement(8850..9899)
                     /locus_tag="PLG72_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79910.1"
                     /translation="MSKHIIELVDVVKDYDGTEALKGISLYIRQNEFITFLGPSGCGK
                     TTTLRLIGGFEKPTSGRILFEGVDVTDVPPYKRKVNTVFQKYALFPHMNVFENIAFGL
                     RIKKLGKDVIREKVSKMLHLVNLEGYEKRSVDSLSGGQQQRVAIARALVNEPEVLLLD
                     EPLGALDLKLRKEMQLELKKMQKQLGITFIYVTHDQEEALTMSDTIVVMQSGTIQQIG
                     TPEDIYNEPKNAFVADFIGESNIIDGIMLRDFRVKFAGHEFDCVDKGFGTNEDVDVVI
                     RPEDIKLVPMDQSPLHGTVKSVIFKGVHYEMQVDDRNGYSWIVHSTRMAPVNTDVGMY
                     IHPQDIQIMKKVSAV"
     gene            complement(9953..10492)
                     /locus_tag="PLG72_03580"
     CDS             complement(9953..10492)
                     /locus_tag="PLG72_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ79911.1"
                     /translation="MEIGHKIKMLRIKNGLTQEELADRSELSKGFISQIERDLTSPSI
                     ATLVDILQCLGTNLRDFFNDSSDEKIVFSKDDAFVKENDGLKHVIRWIVPDSQKNHME
                     PILLELAPGGSSEVDDPHEGEEFGYVLSGSVFIHLAARKYRARKGESFYYKPNSVHYI
                     SNAGKTEAKVLWVSTPPSF"
     gene            complement(10997..11392)
                     /locus_tag="PLG72_03585"
     CDS             complement(10997..11392)
                     /locus_tag="PLG72_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79912.1"
                     /translation="MKNSISIDDITNGLLTIQKVKQLYSRDHRNSEDGSDQYVQPDRL
                     TLMREILTTISRFVPQTRNGTFSSAFEQGMRFSNAYRELKRHVGSMNRSSPGQEEVLR
                     FLKLLKPVLDMRQQVYMDKAVNIIEILRS"
     gene            complement(11531..12826)
                     /locus_tag="PLG72_03590"
     CDS             complement(11531..12826)
                     /locus_tag="PLG72_03590"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ79913.1"
                     /translation="MSIAETCRNVKSAAIKLAACSTEMKNNALAKIAQELAAHKDEIF
                     RANREDLRRSEAENIPMPLLKRLKFDESKLSDCLSGINSLIRLEDPVGRTLMSTELDE
                     GLELYKVTCPVGAIGVIFESRPDALVQISTLCLKSGNGVLLKGGSEAIETNRTLARII
                     ISASESAGIPKGWCSLLETRYEIKEMLKQDEYIDLIIPRGSNEFVRYIMDNTKIPVMG
                     HADGICHCYVDSSADPEMALRIVIDSKTQYVAVCNALETLLVHRDIAKTFLPMLKKAA
                     DEKHVELYGCSETAAVIDVSPATEDDWKTEYLDYKLSVRVVGSLEEAIGHINTYGSGH
                     TDAIVTNDRNNAARFMSLVDSGSVFWNCSTRFNDGFRYGLGAEVGISTGKLHARGPVG
                     LEGLVIYKYMLIGNGHIVADYAEKRRTFTHRKLNRDFPL"
BASE COUNT         3330 a         3135 c         3270 g         3337 t
ORIGIN      
        1 gatgagcagt gatttcaggg gagacccggc ttctgcgatg ctggaggtgc ttgacggcga
       61 gcagaacttc gctttcaggg accattacct ggaactgcct gttgacctgt ccgacgtgct
      121 gttccttacc acggccaatt cgcttgatac gataccgcgg ccgctgctcg acaggatgga
      181 agtaatcacg atttccggct acaccgagga ggagaaagtc cagatagcac agcggtacct
      241 ggtgaacaag cagatgaagg cccacgggct tcagaagaaa aacctgaaga taaccgagga
      301 cgccatcagg tgcatcatca attactacac cagggaagcc ggcgtgagaa acctcgagag
      361 agaaatagcc aacatctgca ggaaagcagc caagctgctg gtttcaggca gcaggaagtc
      421 ggtgactgtc actccgtcaa atatcgagaa gtacctgggg acacggagat acagatttga
      481 caaggccaat gaaaaggatg aagtgggcgt tgccacggga ctggcatgga cgcctgtcgg
      541 cggggatacc cttgcgatag aggtgacggt catggatgga agcggaaagc tggagcttac
      601 gggacaactg ggagacgtga tgaaggagtc ggcaatggct gcggtgagct tcatacggtc
      661 cagggccgaa ctgcttggta tagacaggga gttttacaag aagtacgata tacacatcca
      721 tgtacctgag ggcgcaacac ctaaggacgg gccttcggca ggcataacgc tggcaaccgc
      781 aatggtatcg gcccttacgg gaagggctgt aaaacggaac gtggcgatga ccggtgaaat
      841 aacactcagg gggcgtgtac tgccaatagg cggcgtgaag gaaaaagtgc ttgccgccca
      901 cagggcaggc atcgataccg tgatactgcc cgcggaaaac aggaaggata tagacgaaat
      961 acccgtgaat gtacggtcgg gactgaaatt cgtcttcgtg gaggagatgg atacggtcct
     1021 gaatacagcg ctgaccccgg agaagaatca cagggacatc gaaagcggcc gtgaagacca
     1081 ggaagccgta ctgctcgcag aagaaagcgc agcagtggac ggacagagca gcacgcatcg
     1141 gatgtgatcc tgagagtttt gcatgagacg tttctgccat agcagaagcg tcttttttgt
     1201 tggacggatg ctaaaaaata taaaggaaat gaaggaattc tggcatttaa tatcgaatta
     1261 tgtaaggaat gttatctacg tataaaggca tttgcagatg caagccctga gaaccgctcc
     1321 tgctctgcag gccgaaacca ggaggataac gatgcatgat tatatgattg ctgttatatg
     1381 tgcggtagcg gtcagcggca tcataatgct ggtggttttc caccgccttg aggtcctgag
     1441 tttcaggact gtcgcctcaa tcacaccaat cacactggct tctctgatca cggcattgat
     1501 cataccggcc atatacagcg ctatttccgg cggtgaaact gatagagccg atccgacggt
     1561 tattataatg acaacagtgg cagcatttat cgcttatgtt gtcatagtcc tgatactgag
     1621 cattctgatc tccgtcatcg ttccaggtgt aaaaccccgt gataaggacg ggaaaacttc
     1681 gaagacaaac aaaaaagatg taaaatccga tggaagtatg cgtgaaaaga caatactgga
     1741 acaggttgga gcgctttact tcaatgatag ggcaggagaa agcagagaag aacaggaagg
     1801 ggcagtttcc ggatccggta ctgagcctgt ggctgcgagt atcggctcgg cagaaatcac
     1861 agcagaggca gtgcattcgg agacactccc gggtatgccg gagcagcctg aatccggtca
     1921 ggatatatca tatgcccttg atgatcagct atattcggca ggcatgatgc cggaccatgg
     1981 aacatcgaac cagttggata cagcggccgc ttccgggaca gaaacggaac agtatgcagc
     2041 ctccgtagcg aatgccggat ccggcatgga tacggccaag gtctcggaaa tatcttcctg
     2101 cacggacggg atacagcctg agcatgaagc cgtgcagaca ccggaagttc aggatttccc
     2161 ggatgcgaag ctgcataaac ccttgcttgc cggagacaat taccttgaac agatatacct
     2221 gaattatgtc gcacatggtg acgaattgaa ggaagatgcc ggcgaaatga ccggcgaggc
     2281 tgcgagcgat gcagaaaaat ctgttgacag tgcagaaatt attgataaaa tgggcataga
     2341 aaataaagtt tacgatagcg aagctatgac tatcgaagaa tgtataacag aggctttccg
     2401 gctcaaggaa gccggcgacc ttgaaggagc gatactttat tttatgtacg ctcttgataa
     2461 aaagcctcaa aaggagctta ccttctggat aatacttgac atatgcgtca tgtataaaac
     2521 actcggtcag caggacctgg cactggaaat actcaatggc tattatgagg cttacggcga
     2581 cacgatggat gaccgactca gggacgagat catcaggaat ctgactgatg caggcgctta
     2641 acggacgata tggtctgtta caatattaag gagggtatct ttaaatgaaa aaaactcaaa
     2701 aaatacttgg attgcctatt atcagcatat ccgacggcat ggaagtcgga aaggtcaaga
     2761 gcataattat aaacgctgag aagggagcca ttgactatat tgctgtcgac agcgggatac
     2821 aaattttcag tgccagggtc atagcaacgg aagatatact gggcatcggt gaatatgctt
     2881 tgactattga gaatgaagac gtcattacag atatcagcaa gataccttca gcgatacagt
     2941 tacttcagaa agacataagg gtaaagggaa ccaaggtact taccaaaaag ggcagcctga
     3001 taggcgagat cggtgacata tatgttgatg agaataacgg atgccggata acgggccttg
     3061 aattcatagc agacataaca cagaagcaga tacgcctgat accaagggat tcggtgataa
     3121 cattcggaaa gaatctcacg gtagttacgg aggacgtgga atcaagactt ctcgacacac
     3181 cttcgcagct tgacagtgac agagggtcct tagaatccga aaaaaaaaaa tattattgat
     3241 ttgatcggcc agtttagcgc acaggatgcg gaaccggaaa taaagacagg cctggacggc
     3301 ggctcagcag ttgatgacct ggcaagcgcg cttgatgaag cggcagccat cgccggcgaa
     3361 gaaaccgggt cggaaatcgc tgccggtgcg gacgttccgt ctgagtctgc tgatcagcct
     3421 gcagaagacg acagggacaa tgcgtcgatc ctgttcgaac agaggcagag acagtatctg
     3481 aaagggagat atgcaacaaa gaccataacg gacaacgacg ggaatgtgat catatacgaa
     3541 ggaatgcaga tagacgacgc agtgatcgat gaagcaaaga aaaagagcag gctcgtggaa
     3601 ctggtgatga acaacagggc atgaaagagt aaaaaagtat aaagtacgcg gtgctttata
     3661 cttttttgat aatagtccgg agagccggat actaaagaaa agacttacgg agttgttatt
     3721 tgtgaaaaaa accggtaaca aactgctgat ctttttgcta atactggtac aaagcctgct
     3781 ggcaatagga gcggggattg ctgtcctgtt taatgcttcc ggcagggtta ttccggtcaa
     3841 tgtatatgcg ggagacctgg atataggagg caaaggtttc gaggaagccg ccagggccat
     3901 cgaagctgat tatgaagcca ggttcggagt tcagcatatc cggttgaagg cagacgacga
     3961 tcagatatac gagatacctt tctcgagcat cgaagcaact gttgacggca tggctacgat
     4021 agagccgatt atgtccatgg atgggataga agacataccg cgcctgatcg ggacacattt
     4081 cggcaatcat accaccgttc tgtcacctgt ggtaaaattc aatgaaagca agctcaggat
     4141 gaagctgata gagctgtccg aaagcatcaa cagggacccg gtcgatgccc ggatctacta
     4201 caaggacggg cttattgaaa aggaacctga tgatcccggg gtgtcgctta atgtcaacaa
     4261 tgctgcagag ctgatccgca ggcagctttc cgccgatccc ttcggcgtta tagatctgcg
     4321 ggcagggaaa gccatggata ctgtttacgc agatgtcaca atgaaagatc ttgacgcgat
     4381 acaggtagtg cttggcgaat actcaacgag cataatggat ggagcggtgg aagacagtat
     4441 agagaccgct gtggaaggca taaacgggat cgtgctcgct cccggaggtg atccttcagg
     4501 caggagcgtg ttctcgtttg ccgaaacgat gctttccctg aacccggaca tgacctttga
     4561 cgaaggttac tgccaggtgg cgtcaacgat gtatgccgca ctcctggagg cagggatacc
     4621 ggcggagaat ataacaaggc tgcctcacaa gctgactgtg gattatattg aaccgggatt
     4681 ggacgcgatg gtcgctgagg atgcatacga cctgagattc tcgaacccgt tcggcaacaa
     4741 cctggcaatc tatgctgtaa aaaagggaac cgtcgtaaca gttgctgtcg ccggcagtcg
     4801 tgatgacagg ccggataaga gcgtaatcag cacgcagaca gtaaacaaat cggtaccgcc
     4861 cgtctactat gtggagaaca gggatctgaa accgggagaa caggtagtcc tggaccccgg
     4921 gaaggaatca ctgtaagggt ttacaggaat aacgaactga tcagcacaga tacatatgaa
     4981 gcggaatctt ccatcgtgca gatcggtccc gacactgatc cggaaatcaa aaacaagtga
     5041 cgtgatcagt agaatcttct gaggacttcc agcgcatcgt tttctattat cttctcgcca
     5101 tgctcgctga gataaccttc catgaagctg ttgtttgtta ttttgacgcc gtactccgca
     5161 agcaccagtt cgaacattct catgccgtaa gaatgaaggc cgctttttgt gagcaggaga
     5221 taataggaat ccccgcacct gtacagcgtg ctttcaccgc tgtaatgcat ttcaagcctg
     5281 cgcgcaagct cgcttatgtc gtccatcgat ttgaaagaat atataaggaa cggagatgaa
     5341 acccgcctgc ttttcttctt cacccgcagg tcgcttttct tcatcctgtt cttgatatac
     5401 ttgtggatgg actcgaattc accttcgtcg tcgatccttg tgatattgat gacgaagcct
     5461 tcgtccgaat cgggcacggg ttctatgatc aactgtgagt cggcaaggct gaaacccaac
     5521 tgttcctccg cctgttccat aaggtcccag aaaaactcct gggccgcggg cgagttatag
     5581 ttcagtgatt ccagatctac gttcctctct tcgaggtcat tatatgttat tgtcactctt
     5641 ataatattat cgcctattct ttcgatcttc atggcaactc cggttcagtc ggcatatcat
     5701 aatgccttga ttatatccta cttccattat acttctttat tgctgaaaag agaagcatat
     5761 aattccgcgc ctggccgcca ttatatccat aatggcttat atcccggcaa tctcgggcct
     5821 tttgggagct tcctcccctg catggaaata acggacctgc ggaaattttt catttgcggg
     5881 aaattcctgc cggatctgat cccggccccg ttgctgtttc caattctgca taaaaggttt
     5941 tgatccggtt ctgtcaccag tgctgtacag gatttcgatc ttcggtactc tttctgagcc
     6001 agggcaccat tcaaactgcg gctgagacat aatgaggcgg cgggttatcc cctgccgcct
     6061 cgcctgactt ccgatgactg tgtcaacccg ccttgatttc cgtccagata cggttatata
     6121 ccctgtttat ctcgtctccc ggatattcga agatctcgca gttgacaagc tggtcctcgg
     6181 tcggatatgc actggaattg gtgatcactt ccggatcgag caacttcatg gtctcgacgt
     6241 tgggtgttgc ataaccgata taattcacat ttctgagagc gatctcaggg tcacacagga
     6301 aatttatgaa cagttcagcg gcctcctggt tctttcccgt cttcgggatc accatcgaat
     6361 cgaaccagta gttggagcct tccttcggga tcgcatatgc gagattctca ttttccctca
     6421 tcgcaataag ggcgtcgccg gaccatacca ccgccattgc tgcactctcg gcaatcatca
     6481 tgtcttttgc ctcatccacc agatatgcga gtaccagcgg cttttgctcg atcagttttt
     6541 gcttcgcttc ctcgagttca tcgatgctct tggaattcat ggaataccca agcattttga
     6601 gcgttatgcc gatactgtct ctctcgctgt cagacatgat gatctgtttt ttatattttt
     6661 cgtcccacag tatcttccaa ctgtcaacgg gctccgtcac catggtctta ttgtaaagta
     6721 tgccgaccgt gccccacata taaggcacgg agtacttgtt ttccggatcg aaggcaaggt
     6781 tcttgaactg gtcgcctata tacttataat tggggatgtt gtcaatgttg atctctttca
     6841 gaaggccttc gtcgatcatc ctctttatca tatagtccga cgggatcgca atgtcataat
     6901 tggaactgcc ggtttttatc ttgacataca tgtcttcatt ggtggtatac gtctcgtaaa
     6961 cgatcttgat attggggtat ttatcctcga aatcctcaat aaccgattcg tcgatgtaat
     7021 ctccccagtt gaaaacgttg agggtaactc tctccgtccc gttaccgttg tcagagcctg
     7081 gtgagccgca agctgaaagt gtaaccgcta cagcaagcag caatactgct aaaaatgtga
     7141 tccttttttt cattttcatt tctcctttac tgttgttgta gttgaacacc tcggcttgtt
     7201 gaaaaatctc cttgcctcag tattgtttca aatatctcta tatattcact atcggggctc
     7261 cgttcttgcc gtttgccgag gaacgcttgt tcacgatcag cagcagaacg aggaccacga
     7321 tgaacatcag tgtcgacagg gcattgatct tggggctgat gcccaatctt gccatcgaat
     7381 atatggttat cgaaagcgtg gagactcccg aaccggtagt aaagaaactt attataaagt
     7441 catccagtga cagcgtaaag gccagcagga atcctgtcac gacgcccggc attatctcgg
     7501 gaagaatgac cctgaaaaaa gcgtacgccg gtttggctcc caggtcgagc gccgcctcat
     7561 acatatgctt gttcagctgt ttcagcttcg gaagcactga aagcacgaca tacggtatgt
     7621 tgaacgttat atgggccagc agcagtgtcg taaagcccag atccagtttg atgaaaacga
     7681 acaggatcat gagcgatata cctgtcacta tgtcgggatt tactaccgga agataggtca
     7741 ggttcatcac gaatgatttg accgtctttt tcatattgtg tatcccgact gctgcggccg
     7801 tacctgccac agtagctacc agcgccgaaa gcagggctat tatgaccgta ttatacaatg
     7861 aagacaggac ctgtctgtca tgaaaaagct ctccgtacca tttcagtgtg aaaccgcccc
     7921 agttgccgcg cgactttgat ttgttgaacg aaaaaacgat cagtgtcagc accggcgcat
     7981 acaggaagac gaatataata atcaggtaga tccttttgat tgcctttttc aaaacagcgc
     8041 acctcctgta ccttccttgt cattcctgga catgactccc atgctcagca gtatcagcag
     8101 catcatgatg atcgaaacgg ccgaaccgaa gttccagtcg tataccctca tgaactggtc
     8161 ttcgatcagg ttgcctatca gcatatactg gcttccgccg agcagcttag aaattatgaa
     8221 tgtagttacg gccggcatga acaccatcgt tacgcctgaa gcgactccgg gcagactgag
     8281 cgggaatatg accttccgga acaccgttaa gttgtccgac ccaaggtctt cagcggcttc
     8341 gatcaggctc ctgtcgatct ttatcagcac cgagtatatc ggcagcacca tgaaaggcag
     8401 gaaattgtaa accatgccga gaacgacagc aaaatcggta tacagcaggt gtacctgcgg
     8461 caggcccaat gtccccagga tggagttgat cacgcccttg cggtccagca atgtcatcca
     8521 tgcatatgtc ctcagcagga aattcatcca catcggtacg accatgagca tgaccaatgt
     8581 gcttttcctg ctgatatctt tgccggcaag tatcattgcc acaggatatc ctaaaatgaa
     8641 gcagacgacc gtactgatca ccgcgagcac tattgatctc cacaaaactt tcagatatgt
     8701 gggctccatc acccttatga aattatccag cgtaaatacc accgtgccgt tttctgtcct
     8761 ggtaaagctg tagtacagca ccagcagcag cggcacgata gtgaatatta tcatccataa
     8821 tatatacgga tatgacaccc atgtcttttt catactgccg acaccttttt cattatttga
     8881 atatcctgcg gatgtatata cattcccaca tcggtattga ccggggccat ccttgtgctg
     8941 tgcactatcc agctgtaacc gttgcggtcg tccacctgca tttcataatg gactcccttg
     9001 aatatcaccg atttgaccgt gccgtgcagc gggctttggt ccatcggtac cagttttata
     9061 tcctcggggc ggatgaccac gtcgacatct tcatttgtgc cgaagccttt atcgacacag
     9121 tcgaactcgt gcccggcaaa tttgaccctg aaatccctca gcatgatccc gtcgatgatg
     9181 ttgctttcgc ctatgaagtc ggcaacgaaa gcatttttgg gctcgttgta tatgtcttcg
     9241 ggagtaccta tctgctgtat cgtgccgctc tgcatcacga cgatggtatc cgacatggta
     9301 agggcttctt cctggtcgtg cgtgacatat atgaacgtga tcccaagctg cttctgcatt
     9361 ttcttgagtt cgagctgcat ttccttgcgg agctttaggt ccagcgcgcc gagaggctcg
     9421 tccagcagaa gcacttcggg ttcgttgacc agcgcgcgcg ctatggcgac cctttgctgc
     9481 tggccgccgc tcagcgaatc caccgaacgt ttctcgtagc cttccaggtt caccaggtgc
     9541 agcattttgg agactttttc acgaatcacg tctttgccga gtttcttgat cctgagcccg
     9601 aatgcgatat tttcaaatac attcatatgg ggaaacaggg cgtatttctg gaagacagta
     9661 ttaaccttcc ttttgtacgg cggcacgtcc gtgacatcga cgccttcgaa caggatcctg
     9721 cctgaggtgg gcttttcgaa accgccgatc aggcgtaagg tagtagtctt tccacatccg
     9781 ctgggaccca ggaacgttat gaattcgttc tgacggatat acaggctgat acccttcagc
     9841 gcttcagttc cgtcataatc ttttaccaca tccaccaatt cgataatgtg ttttgacata
     9901 gcgcatactc cttataccag atagtcaagt atgccggtgt tcttatggca actcagaaac
     9961 tgggcggggt ggatacccat aatacctttg cctctgtttt cccggcgttt gatatatagt
    10021 ggacggaatt gggcttgtag taaaaactct cgccttttct cgcccggtat ttccttgctg
    10081 ccagatggat gaaaacgctc cctgacagta cataaccaaa ttcctcgccc tcgtggggat
    10141 catccacttc cgaagatcca cccggggcga gttccagcag tatcggttcc atgtggtttt
    10201 tctgcgaatc cggcacgatc catctgatca catgcttaag gccgtcgttt tccttgacga
    10261 aggcgtcatc cttactgaat actatcttct cgtcggaact gtcattgaag aagtccctca
    10321 ggttcgtgcc caggcactgc agaatatcca caagagtcgc tatcgacgga gaggtgaggt
    10381 ccctctctat ctgcgaaatg aagcctttcg aaagttcgct cctgtctgca agctcttcct
    10441 gtgtcaaacc gtttttgatc ctcagcattt tgattttgtg gcctatttcc ataccattat
    10501 tttcctttca ctatccagac gcctaccgga tctttttgct tttataagtt atatctaaac
    10561 attttgttta caatcactaa acaaaatgca aaaagcaatt tattataatc acataatata
    10621 tttgatgtca ataatatttt tcaaaaaaag catgagaaga tataccggta tggcgggcaa
    10681 aatttccggc aggtccggat tgccgggcgt attttcgtat atgaattatt ttaagcaatt
    10741 gatgagcagg tgatgactgg tgttattgct gaatatgtga acatacccga aggcagcaga
    10801 cggcaagtgt acagatttgg ggcaggaact gcattgtgta cagattgagc gtttcaactg
    10861 tccgcaggta cttccttgat gatcagaaat tgcccggcgt acagatccgg ggatattacc
    10921 tgcatggcga ttgatatata cttttgcgtg tataggtcaa gagtaagggg aattagttgt
    10981 cctgtgatcc tgatgcttac gatctcagta tttcgattat atttacagcc ttatccatat
    11041 aaacctgctg gcgcatgtcg agcaccggct tgagcagctt caggaatctc agcacctctt
    11101 cctgtccggg cgaactcctg ttcatactcc cgacatgccg cttcagttcc ctgtaagcgt
    11161 tgctgaaacg catcccctgt tcgaatgcgc tgctgaaagt cccgttcctt gtttgcggaa
    11221 cgaacctgga tatcgtcgtg agtatctcgc gcatgagagt aagcctgtcg ggctggacgt
    11281 actggtccga cccgtcttca gagttcctgt ggtccctgct gtaaagctgt tttactttct
    11341 gtatggtcag cagtccgttg gtaatatcat ctatgcttat tgaatttttc atggtatcac
    11401 cgttcttaac tgttttgata catacggcaa tgttttgacg cgttgctgcg gagcaaaaca
    11461 taataaaatc gccgcctacg ttcatattat gttaaacagg cggcaatgtt tcttaatggc
    11521 gattccaatt ttacagcgga aaatccctgt tgagtttcct gtgggtgaat gtcctgcgtt
    11581 tttcagcgta gtccgccact atgtgcccgt tgcctataag catgtattta tatataacaa
    11641 ggccttcaag acctacaggg cccctggcat ggagttttcc cgtactgatg cccacctccg
    11701 cccctagtcc gtacctgaac ccgtcgttga acctggtgga gcagttccag aagacactgc
    11761 cggaatcgac cagcgacatg aagcgtgcgg cattgttcct gtcattggtg acaatggcgt
    11821 cggtatgtcc cgaaccatac gtatttatat ggcctatggc ttcttcaagg ctgccgacga
    11881 ccctgacgga cagcttgtag tccaggtatt ccgtcttcca gtcgtcttcc gttgcagggc
    11941 tgacatctat gacagctgct gtttcgctgc agccatacaa ttcgacgtgt ttctcatctg
    12001 ccgctttctt cagcatgggc aggaaggttt tcgcaatatc cctgtgtacc aggagcgtct
    12061 ccagagcatt gcagaccgct acatactggg ttttggagtc gatgacgata cgcaatgcca
    12121 tttcaggatc ggcggaggaa tcaacataac agtggcatat gccgtctgca tggcccatga
    12181 cagggatttt tgtgttgtcc attatatacc tgacaaattc atttgagcct ctgggtatta
    12241 tcagatcgat gtactcgtcc tgcttcagca tttcttttat ctcgtaccgg gtttccagta
    12301 ggctgcacca tcctttgggt atccctgcgg attccgaggc ggagattatg atcctggcca
    12361 gcgtcctgtt agtttcgatg gcttcggatc caccctttaa aagcacgccg ttcccgcttt
    12421 tgaggcagag cgtcgatatc tgcaccagcg catcaggccg ggattcgaat atcacgccga
    12481 tcgctccgac agggcaggtc accttgtaaa gctcgagacc ctcgtcaagc tcagttgaca
    12541 taagtgtcct gcctaccggg tcctcaagcc tgatgaggct gttgatgccc gatagacaat
    12601 cgctgagttt tgactcgtcg aacttgagcc tcttcaggag cggcatcggt atattctccg
    12661 cttcgcttct tctcaggtct tcccggtttg cccggaatat ctcgtcttta tgggcggcaa
    12721 gttcctgcgc gatcttcgca agagcgttgt tcttcatttc agtggagcat gccgccagct
    12781 ttatggctgc tgattttaca ttacggcagg tttcggcaat actcattcaa aacaccccgc
    12841 atcgatggtt tttataagta tatcaaatgc gatgccccat atcaagctgt gagaggatgg
    12901 aggcaaacgg caggcggcag gcggcggact gcataggtgc caggcggtga aaagtacacg
    12961 cagcacgggc cggctgcggg ccgatgccgg tttcctgcgg ccggaaatat tgcatggcac
    13021 cttagtgtaa aattgtttta ggttaaaaaa acaagagacc cctctttttt gg
//
LOCUS       DAOSCC010000040        12935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3265_length_12935_cov_4.827407, whole genome shotgun sequence.
ACCESSION   DAOSCC010000040 DAOSCC010000000
VERSION     DAOSCC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12935
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_12935_cov_4.827407"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLG72_03595"
     CDS             complement(<1..454)
                     /locus_tag="PLG72_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOJ79914.1"
                     /translation="MKKAAFYTLGCKVNQYETEAMAEAFEKAGYTVVDFEEAADVYVI
                     NTCTVTGLSSRKSRQAIRRAKQRNGNAVVAAVGCYPQTAKEELESMPEVDIIVGTAER
                     RRLPEYVEEFLKGNVRITAVGNIMKNRSFEDLRIEKYKGRTRAIMKVQE"
     gene            621..848
                     /locus_tag="PLG72_03600"
     CDS             621..848
                     /locus_tag="PLG72_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ79915.1"
                     /translation="MKSFNIMLKTINDVKDFVNIANKYDFDIDLTSGRYVVDAKSIMG
                     IFSLDFNKPIKVDVHSDDCEDFCSEIRPFMV"
     gene            complement(990..1460)
                     /locus_tag="PLG72_03605"
     CDS             complement(990..1460)
                     /locus_tag="PLG72_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOJ79916.1"
                     /translation="MYIVSACLLGISTRYDGGCFCDQKLMKLAEEGKAVPVCPEQLGG
                     CQTPRDPCEISGGDGGGVLDGRCAVISRNGTDMTEKFIKGAYETLKIAKTCGAKKAIL
                     KARSPSCGTGRIYDGTFSGKTVQGNGVAAELLIRNGIEVYSEETAEDALTEDAP"
     gene            complement(1414..3603)
                     /gene="rnr"
                     /locus_tag="PLG72_03610"
     CDS             complement(1414..3603)
                     /gene="rnr"
                     /locus_tag="PLG72_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOJ79917.1"
                     /translation="MDILQERKDKIVAFMKEAAYKPLKFSELAVVLDVPHEDRDKLAE
                     ILDSLEREGVIYKTKKERYGITEKMNLVVGRIQRNERGYGFLVPDDGSVPDVFVPADG
                     LAGAMHNDKVIVRITSKGANGKRAEGEVIKILERSVTKVVGTFENSRYFGFVVPDDKK
                     MPGDVFIPKDETNGAKPGQKVVAQIVKWPDGRRNAEGKIVEIIGDSKDTGVDILSIMK
                     AHDLTESFPDEVMAQANRIPDVVTDDMIKGRRDLRDLRTFTIDGEDAKDFDDAVSIEV
                     LPDGNYRLGVHIADVSYYVTEGSPLDREALKRGTSVYLVDRVIPMLPPKLSNGICSLK
                     PGVDRLAFTVMMDIDAGGKVIRHDIFESVIRSSERMTYTDVYKILETGDEELTERYSH
                     LVEDFRKMKDLALILRSRRMARGAMDFNFDEAKVILDENGKPVDVRRYEITIANRIIE
                     EFMLVCNETVAEHFFWTGLPFVYRIHEEPDTEKIMAFAEFAKNLGYPLKGVNNIHPGA
                     LQDVLERVKGKKEELIVSTVMLRSLAKAKYSHQNAGHFGLAARYYCHFTSPIRRYPDL
                     IIHRLIKEQLKGGISEEREQELSERLPEVARKCSERERAADEAERDTEELKKVEYMKQ
                     KVGEVFDGIISNVTSFGLFVGLENTVEGLVRISDMHDDYYIYDDKRYMLIGERTGKCY
                     RIGDPVRVLLAKADVETRQIDFVLEEHVKGGSDVHRKRLPARHKHPL"
     gene            complement(3789..4340)
                     /locus_tag="PLG72_03615"
     CDS             complement(3789..4340)
                     /locus_tag="PLG72_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ79918.1"
                     /translation="MDRDQALIELKARLKNENKLKHSLAVEAIMRKLASRLKGDAETW
                     GIVGLLHDIDCDRTANEPEKHSLLGAEILENIGLDEDIVYAVRAHNPIHGIERRRKMD
                     KALFCADAVSGFIMACALARPSGRLSDVTAGFVMEKIDDGSFAEDTDRDRIRSCSGLG
                     LPPDEFIGIALAAMGEISDQLGL"
     gene            complement(4463..4702)
                     /gene="secG"
                     /locus_tag="PLG72_03620"
     CDS             complement(4463..4702)
                     /gene="secG"
                     /locus_tag="PLG72_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ79919.1"
                     /translation="MNAGQIIVTILHVIISLAMVVIVLLQSGKSAGLSGTIAGGAETF
                     FGKNKGRTLDAILSKYTSAVAVAFLITSVVLVLIL"
     gene            complement(4774..6075)
                     /gene="eno"
                     /locus_tag="PLG72_03625"
     CDS             complement(4774..6075)
                     /gene="eno"
                     /locus_tag="PLG72_03625"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ79920.1"
                     /translation="MKQYLEIESVFGREILDSRGNPTVEVEVIAEGGFVGRAAVPSGA
                     STGAFEAIELRDGDKGRYLGKGVLKAVSNVNEIIAPEVEGMNVFDQVAIDRKMIALDG
                     THNKSRLGANAILGVSLAVAKAAAEALGLSLYQYIGGVNAKTLPVPMMNIINGGKHAD
                     NSVNIQEFMIMPVGADCFKNALMMCAEVFHNLKKVLSDKGYSTAVGDEGGFAPNLKND
                     EEAIKAILEAIEKAGYRPGDDFRIAIDAAATEMYREDGTYHFWKTNITKTREEMVEFW
                     ADLANKYPIISLEDGVAEEDWEGWKLLTKKLGDKIQLVGDDLFVTNTERLKKGIEMGV
                     ANSILIKVNQIGTLTETLDAIQMANRAGYTAVTSHRSGETEDTTIADIAVATNSGQIK
                     TGAPSRTDRVAKYNQLLRIEEELGTSAIYPGLDAWFNLKNK"
     gene            complement(6534..7418)
                     /locus_tag="PLG72_03630"
     CDS             complement(6534..7418)
                     /locus_tag="PLG72_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ79921.1"
                     /translation="MIECENLVKIYKTKDIEVVALQGLDLIVEKGELMAIIGNSGSGK
                     STLLNMLGGLDRPSAGKLFVDGHDLLKFNEQQLIKYKRETVGFVWQNNARNLIPYLTA
                     QENVEMPMILTGKKNRRNRALQLLDLVGLLNRRNSKLSQLSGGEQQRVAIAIALANDP
                     KLLLADEPTGSVDSRTASAILDVFRELNKALGVTVVIVTHDRQISRKVDRVVAIRDGR
                     TSSEFIRKRSYAEELAALGDKVFSDQSDTHDELVVLDRAGRLQIPAEYMQALGLKGKN
                     KVKVELEGDRVVVYAPKE"
     gene            complement(7492..8172)
                     /locus_tag="PLG72_03635"
     CDS             complement(7492..8172)
                     /locus_tag="PLG72_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79922.1"
                     /translation="MSAIIKTENLSRDFKSGNTVVHALKNVSIEIEKGKLTILRGRSG
                     SGKTTLINLLGALDRPDSGTVVFNGIDITRAGERVRNELRRKHMGFVFQSVALISMMS
                     AYENVEFGLRIAGAGAKGRSERARKCLSLVGLAKRMDHRPHELSGGEQQRVAIARAIA
                     HRPDVVFADEPTAELDTLMGLQVVKLFRELVELEGLTVVMTTHDPSMIEIADHVLTLE
                     DGEIIDAR"
     gene            complement(8186..11062)
                     /locus_tag="PLG72_03640"
     CDS             complement(8186..11062)
                     /locus_tag="PLG72_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ79923.1"
                     /translation="MLKIVLRRMFSNSWLIICLLLGSIMATALVSCIPIYTDGIMQRL
                     LTKDLENYQLETGDFPGQYSVRARFTYKNDYSERISNQRYFNKEIMTNKITDFGLPIK
                     SASHHVVLGSVSFFPRPVENASEYDYRTKTVYSRLGALSGLEDHITITKGRMYSDQIT
                     EDGAIEVIVSQQAMVILDLLLDREYTLFDPARDYAEVCKVKVVGIYEMKDPGDVYWLH
                     EFSMHNKEFMMDYGLFNDVFIAPKTGPLTEAEWYCAFDYHAITLQNLAHVVRTYEEQE
                     KWFSKYKVSIEFEMPINKILAKYNERARQLQNMLWVLTVPVLIMLTFYIFMVSQLIID
                     HDENEIAVFKSRGARKGQIFLSYLIESSIVAAVAYLAGPFLGLLICKILGASNGFLEF
                     VQRTALPVQLSPKVYMYSLFAVLFLIVTMLAPVISVSRKTIVSHKQEKARARKAALWK
                     KMGLDIVLLLIAGYGIYRYRQQQRVLEATGISANELQIDPLLFLTSTAFVLGAGLLFI
                     RLYPYLIRLIYWLGRKVWTPVTYISLIQVGRSRGQEQFLMLFMILAISIGLFNANTAR
                     TVNKNMEDKIRYSVGADVRLQAIWKTNQKNAPQGAQSPTMAQMQAQMQSDEPLRYYEP
                     NFNDYATLSGAEVVTKVLNVDNGTMQTDMGVASGLKVMGIIPHEFAQVAWFRRDLLPY
                     HINDYINLMSEAPTAFLVSSNLKDKYKLELGDTIYVTWGSQSYLEGTIYAFIDYWPTY
                     NPKADRNLGGEAGLVVVNYSYIQNKMALQPYEVWIKKADGATDKQINDDIIKKDLELS
                     FIKYTDQELIKMKNDAMVQGMNGMLTLGFIASMLISLLGFLIYWIISIQNRTLQFGIL
                     RAMGLSLAKVIGMLACEQLLVSGAAIIMGIIVGGIAGDIFIPLLQIVYSAAEQVPPFR
                     ITAYGEDYIKIYAVIGIMITIGFMTLWRIIAKIRIDQAVKLGED"
     gene            complement(11151..12191)
                     /locus_tag="PLG72_03645"
     CDS             complement(11151..12191)
                     /locus_tag="PLG72_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ79924.1"
                     /translation="MYNSEKKRSGTIMSKCILILLMAALSVMTLTGCYFFPKEEEELA
                     PPIKVPANITYETVEVTRGTIENVIRVTGNFVSVSQKNVYFVKSDRLKGIYVSSGQQV
                     KKGDLLAELDTESLMNEIKLQEIALERSQILYEEAKANYEIVGGSRTSLDLAELDLES
                     NRVKLENLKSQLQKARLVSPIDGTVVYVASVKEGDYVNAYQTIVRVADPTKLQLRYSG
                     DRSTSFRIGMDVAVEINNETYDAEIVMTPYEAPDDADEEAKKSVQIEVYGLPGEIRMG
                     QPASILLTLERKENVIVIPKLLVNTFANRKFVNVLNDGVREERDIEVGIQTSTEVEVV
                     KGLQEGELLIVK"
     gene            12375..>12935
                     /locus_tag="PLG72_03650"
     CDS             12375..>12935
                     /locus_tag="PLG72_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79925.1"
                     /translation="MNLFPSRNGKTPQKSPGISRLYMRLKSRITLYFFIMVILPSIAA
                     ALAIYMHTRSLMVQASYEAALTRITKENNYIGYCLKELELAVDSSVNSISPQQLIQLR
                     EGSNHISNEGILADMADKCLSPAQRKSLSAIYVADSEGIRASYGPDADKAVISGLSSQ
                     KWFGDALVNGRKVLDGIDWTSAGNYIV"
BASE COUNT         2757 a         3671 c         2900 g         3607 t
ORIGIN      
        1 cctcctgcac tttcattatc gcgcgggtac ggcccttgta tttttctatc cttaggtcct
       61 cgaaggaacg gtttttcatt atgttcccca ccgcagttat cctgacgttc cctttaagga
      121 attcctccac atactcaggc agcctgcggc gctcggcagt cccaacgata atgtcgacct
      181 caggcatgct ctccagttct tccttcgcag tctgcgggta gcatccgaca gcagcaacga
      241 cggcattgcc gttcctctgt ttagccctcc gtatagcctg gcgggatttc ctggaactga
      301 ggcccgtgac ggtacaagtg ttgatgacat agacatcggc ggcctcttcg aaatcgacca
      361 ccgtataacc ggccttttcg aaggcctcgg ccattgcctc ggtctcatac tggttcacct
      421 tgcatccaag tgtataaaaa gcagctttct tcacgattcc tccaatatag atctcaatta
      481 cccgttattt cattcagctc aatattaatt cacacttttt taattttctc gtaattgact
      541 tgttaccaaa tacaggatat catttaaata accctgtata catagtatat accaaccttt
      601 agagcaaagg aggcggcaca atgaaatcat tcaatattat gcttaaaacc atcaacgatg
      661 tcaaagactt tgtaaatata gccaataaat acgattttga tattgacctc acatcagggc
      721 gttatgttgt cgatgcaaag tcgataatgg ggatcttcag tctcgacttc aacaagccga
      781 tcaaggtgga cgtacactcg gacgactgcg aagacttctg cagcgagatc aggcctttca
      841 tggtctgatg gactggatcg cgatccggcg cataaaaact ggaacgcatg atcctgatat
      901 gaaatgcgtt atccggatgt gaagtgcgag atccgaatct gcaacacgaa acccggtgat
      961 aaagcagccg ggtttttttg cgtctcctgt tatggcgcgt cttctgtgag cgcatcctct
     1021 gcagtttcct cgctgtacac ctctatgccg ttcctgatga gcagttcggc agccacgccg
     1081 tttccctgaa ccgttttccc gctgaaagtg ccgtcataga tccgccccgt gccgcaggac
     1141 gggcttctgg ctttcagtat cgcctttttc gcgccgcagg tctttgcgat cttcagggtt
     1201 tcatacgcgc cctttatgaa tttttccgtc atgtccgtac cgttcctgct tatgactgcg
     1261 cacctgccgt ccagcacgcc gccgccgtct ccgccggata tctcgcaggg atcccttggc
     1321 gtctggcatc cgccaagctg ttccgggcaa acaggaacgg cctttccttc ttccgcgagc
     1381 ttcatgagct tctgatcgca gaagcatccc ccgtcataac gggtgcttat gcctagcagg
     1441 caagcgctta cgatgtacat ccgatcctcc ctttacatgt tcctcgagga caaagtcgat
     1501 ctgcctggtc tccacatcgg ccttcgcaag cagcacacgg acaggatcgc ctatcctgta
     1561 gcatttgccg gtgcgttcgc ctatgagcat gtatcgcttg tcgtcatata tataataatc
     1621 atcatgcatg tcgctgatcc tcacaaggcc ctccacggta ttctcgagcc cgacgaacag
     1681 gccgaacgag gtcacgttgg atatgatgcc gtcgaacacc tctccgacct tctgcttcat
     1741 gtattcgacc ttcttcagtt cctcagtatc tctttccgct tcgtccgcgg cccgctcccg
     1801 ctcggaacat ttccgcgcga cctccgggag cctttcggac agctcctgtt cacgctcttc
     1861 ggaaatgcct cctttaagct gctcctttat aagcctgtgt atgatcaggt ccggataccg
     1921 cctgatggga gatgtgaaat gacagtagta cctggcggca aggccgaaat ggcctgcgtt
     1981 ctggtggctg tattttgcct ttgccagcga cctcagcatc actgtgctga cgatcagttc
     2041 ctccttcttg ccctttaccc tttcgagcac atcctgcagc gctccgggat gtatgttgtt
     2101 cacgcctttc agcggatatc cgaggttctt cgcgaattcc gcgaacgcca taatcttttc
     2161 ggtgtcgggc tcttcatgta tcctgtaaac gaaaggcaag cctgtccaga agaaatgctc
     2221 ggcaacggtc tcattgcaga cgagcatgaa ttcctctatg atcctgttgg ctatggtgat
     2281 ctcatatcgc cttacatcca caggctttcc gttttcatca agtatcactt ttgcctcgtc
     2341 gaaattgaaa tccatcgctc ccctggccat tctccttgaa cgcagtatca gggcaagatc
     2401 cttcatcttc ctgaaatctt cgaccaggtg gctgtaccgt tccgtcagtt cctcgtcccc
     2461 tgtctcaagg atcttgtaaa cgtctgtata tgtcatcctc tccgaggacc ttatcacact
     2521 ctcgaagata tcgtgcctga tcaccttccc tccggcgtcg atgtccatca tgaccgtgaa
     2581 cgccagtctg tccacacccg gtttcaggct gcatatcccg ttggaaagct tgggcggcaa
     2641 catcggtatc accctgtcca caaggtaaac gcttgtgcct cttttcaggg cctccctatc
     2701 cagcggcgac ccctcggtaa cataatagct gacatccgcg atatgcacgc caagcctgta
     2761 attaccgtcg ggaaggactt ctatggacac tgcgtcatcg aaatcctttg cgtcctcgcc
     2821 atcgatggtg aaagtcctga gatccctcag gtcccgtctc ccctttatca tatcgtcggt
     2881 cacaacgtca ggaatcctgt tggcctgcgc catcacttca tcagggaacg attcggtcag
     2941 atcgtgggcc ttcattatgg acaaaatgtc aacaccggta tctttgctgt cccctatgat
     3001 ctccactatc tttccttccg cgttgcgcct tccgtcaggc catttcacga tctgcgccac
     3061 tactttctgg cccggttttg cgccgtttgt ctcgtccttt ggtatgaaaa cgtctccggg
     3121 catctttttg tcgtccggca ccacaaaacc gaaatacctg ctgttctcga acgtgccgac
     3181 gactttggtc acagagcgct ccagtatctt tatgacctct ccttcagccc ttttgccgtt
     3241 tgcccctttg gatgtgatgc ggactatgac cttgtcattg tgcatcgctc ccgccagtcc
     3301 gtcggcgggc acgaaaacat cgggaacgct cccgtcgtca ggcacaagga agccgtagcc
     3361 gcgctcattc cgctgtatgc gtcccacgac aaggttcatc ttttccgtta tcccgtatct
     3421 ttcctttttt gttttatata tcacgccctc ccgttcgagc gaatccagta tctcagccag
     3481 cttgtccctg tcttcgtgcg gcacgtccag caccacggca agctcgctga acttgagggg
     3541 cttgtatgcc gcctccttca taaaagcaac tatcttatcc tttcgttcct gcaatatatc
     3601 catcaaaatc ctcctgttgt ttgtctgtaa gacgatttgg ctgccgtttt cctgcgtcat
     3661 ccctgttatt cagccagtcc tgtctgtcaa tgagcgcgtt ttatccggcc tgctattatt
     3721 atcccagtat aagatggttt taaactaaaa acgagggtcc caacccccgt ttcgtatcgt
     3781 atctatgatc ataagcccaa ctggtccgat atctctccca ttgccgcaag cgcgatcccg
     3841 atgaattcat ccggcggcag gccaagtccg ctgcaggacc tgatccggtc cctgtctgtg
     3901 tcttctgcga acgatccgtc atctatcttt tccattacga acccggccgt gacatctgaa
     3961 agtctacctg acggccgggc cagtgcgcat gccattataa agcccgatac cgcatcggcg
     4021 caaaacagcg ccttgtccat tttccttctc ctctcgatgc catggatggg attgtgtgcc
     4081 cttaccgcat atacgatatc ttcgtcgagc ccgatgtttt caagtatctc ggctcctaga
     4141 aggctatgct tttccggctc gtttgccgtc ctgtcacaat cgatgtcgtg cagcagaccg
     4201 acaatgcccc atgtttcggc atctcccttc agccgtgagg caagctttct catgatggct
     4261 tcgacagcca gcgaatgctt cagtttattt tcgtttttca acctcgcttt cagttcgatc
     4321 agggcctgat ctctgtccat accttcacct ggcttcccaa taatgtgtgc ctgagctcat
     4381 cctggtcgtc ccggtgtatt tggaatcctg ctgtatcata aaaaagacca tgcatttctg
     4441 cacagtcttt tgatattctc tctcacagga tgaggaccag cactaccgat gttataagaa
     4501 atgcaaccgc aacagcggat gtgtacttgc tcagtatggc atccagcgtc ctgcccttgt
     4561 tctttccgaa aaacgtttcc gctcctcctg cgatcgtgcc tgacaacccg gccgacttgc
     4621 cggactggag aagcactatg acgaccattg cgagcgagat tattacatgc agtatcgtta
     4681 ctattatctg gcctgcattc aaaaggcaac acctcccgaa aaatcaacaa atcacattgt
     4741 aacacatatc ccgtgaaaaa acaagcatga gatctacttg ttcttcaggt tgaaccatgc
     4801 gtcaagtccg ggatagatcg ccgatgtgcc aagttcttcc tctatcctga gcagctggtt
     4861 gtacttggcg accctgtcgg tgcgtgaagg cgctcctgtc ttgatctggc ctgaattggt
     4921 cgcgactgcg atatctgcga tggtagtgtc ttcggtctca ccggacctgt gggaggtgac
     4981 tgccgtatat cccgccctgt ttgccatctg gatggcgtcc agagtttcag tcagtgtgcc
     5041 gatctggttg acttttatga gtatggagtt ggccacgccc atttcgatac ctttcttaag
     5101 cctttccgta ttggttacga acaggtcgtc accaacaagc tggatcttgt cgccgagctt
     5161 tttggtaagc agtttccagc cttcccagtc ttcctcggcc acaccgtcct caagcgaaat
     5221 gatcgggtat ttgtttgcaa ggtctgccca gaactcgacc atttcctcgc gggtcttcgt
     5281 tatattcgtc ttccagaaat ggtatgtgcc gtcttccctg tacatctcgg tcgccgcagc
     5341 gtcgattgcg atgcggaagt cgtcgcccgg cctgtatccg gccttttcga tcgcctcaag
     5401 gatggccttg atggcttcct cgtcgttctt gaggttaggc gcgaatccgc cctcatctcc
     5461 gaccgccgtc gaatatccct tgtcgctcag cacttttttc aggttgtgga acacctcggc
     5521 gcacatcatc agggcgtttt tgaaacaatc cgctccgaca ggcattatca tgaattcctg
     5581 gatgttgacg ctgttgtcgg catgctttcc gccgtttatt atgttcatca tcggtaccgg
     5641 cagggtcttt gcgtttacac cgccgatata ctggtagagg ctaaggccga gggcttccgc
     5701 tgctgccttg gccactgcca gcgaaactcc gaggatcgcg tttgctccga gcctgctctt
     5761 gttgtgggtg ccgtcaagcg cgatcatctt cctgtctata gccacctggt cgaacacgtt
     5821 catgccttcg acctcgggag ctattatttc gtttacattc gagactgctt tgagtacacc
     5881 cttgccaagg tatcttccct tgtcgccgtc tctcagttcg atcgcctcga aagcgccggt
     5941 cgatgcccct gaaggtacgg ccgcgcgtcc cacgaagcct ccttcggcta tgacttctac
     6001 ttcgacagtc gggttgcctc ttgagtcaag tatttcacgt ccaaatacac tttcgatctc
     6061 cagatactgt ttcatgatcc atgctccttt cgattccttt attatatata aataatgccg
     6121 gttttatcat acccgtatgg ccgggcttgc aaacgaccct tccatcgaca tgatgtccat
     6181 acctgtccgt tccatattat accaccgaca aatgtgcatg tccacgccga taaagccatc
     6241 cggtatattt tatccataaa aggcggaaat caaacaaaaa tcaccttccc aatcacccca
     6301 tacaacactt aaatagcccc ccttttcatc agaacaatat gatagtctgg ttataccacc
     6361 gggacggtcc ccgtgtctcc ggaaatcgga acggtatgcc ctgagcagat gtataataaa
     6421 accttccggc actgtgcaaa tacagttgcc ggaaggtttt ttcacacatg tcgacaacat
     6481 ctttgatata tgcgcccatg cactcgtggg acacgagaac cctgtatccc ggatcattcc
     6541 ttcggagcat atacgacgac cctgtcgcct tcaagttcga ccttcacctt gtttttgccc
     6601 ttcaggccga gggcctgcat atattcggca gggatctgga gcctgccggc tctgtccagc
     6661 acgaccagtt cgtcatgcgt atccgactgg tctgagaaaa ctttgtcgcc caatgccgca
     6721 agctcctccg cataagatct ctttcttatg aattcgctgc tggtccgtcc gtcacggatg
     6781 gccacgaccc tgtccacctt gcgcgatatc tgcctgtcgt gcgttacgat gaccaccgtt
     6841 acgccaaggg ccttgttcag ttcgcggaaa acatcaagta tcgctgacgc agtccggctg
     6901 tccacggacc ctgtcggttc gtccgcaagc agcagtttcg gatcgttggc cagtgcgatg
     6961 gcaatggcga ccctctgctg ttcgccgccc gaaagctggc tcagcttgct gttgcgcctg
     7021 ttcagcagcc cgacgaggtc aaggagctgc agcgcgcggt tccgcctgtt ctttttgcct
     7081 gtcagtatca tcggcatttc aacattttcc tgggcggtga gatatggtat caggttcctc
     7141 gcattattct gccagacaaa gcctaccgtt tccctcttgt atttgataag ctgctgttca
     7201 ttgaatttca gcagatcatg cccgtccacg aacagtttgc ctgccgacgg cctgtccagc
     7261 ccgccgagca tgttcagcag cgtggacttg ccgctgccgc tgtttccgat gatagccatc
     7321 agttctcctt tttccactat gagatcgagt ccctgcaggg ctacgacttc gatatccttc
     7381 gtcttgtata tctttaccag gttctcgcac tcgatcatcc tggcccgatc accctggccg
     7441 ccggtctcga tcccttccgc tgtctcatcg ggcttattca tatcgatacg ttcatcttgc
     7501 atcaattatc tcaccgtcct ccagcgtcag tacatgatcg gcgatctcga tcatgctcgg
     7561 gtcgtgcgtt gtcatcacca ctgtcagccc ttccagttcc acgagttccc tgaacagttt
     7621 cacgacctgc agacccatga gggtgtccag ttcggcagtc ggctcatcgg caaacacgac
     7681 atccggcctg tgggctattg ccctggcaat agccaccctc tgctgttcac cgcctgaaag
     7741 ttcatggggc ctgtggtcca tccttttcgc cagcccgaca agagaaaggc acttcctggc
     7801 cctctcgctc cttcccttcg cgccggcgcc tgcgatcctc agtccgaatt ccacgttttc
     7861 atacgcagac atcatggaaa tcagtgccac agactggaac acgaaaccca tgtgttttct
     7921 tctgagctca ttcctgaccc tctctcctgc gcgcgttata tcgattccgt tgaagacgac
     7981 cgtcccggaa tccggcctgt caagcgcccc cagcaaattg atgagcgtag tcttgccgga
     8041 acccgaccgg cccctcagta tagtaagctt tcctttttct atctctatcg atacgttttt
     8101 cagtgcatgg actacggtat tccccgattt gaaatccctc gacaggtttt cggttttgat
     8161 gattgcgctc atagtccgac ctccgtcaat cttctccaag cttgactgcc tggtctatcc
     8221 tgatttttgc gattatcctc cacagggtca tgaagcctat ggtgatcatt atgccgataa
     8281 cagcataaat ctttatataa tcctctccgt aagcagttat tctgaacgga ggcacctgct
     8341 ccgcggcgct gtacactatc tgcagcagcg ggatgaatat gtcgcctgct atgcctccca
     8401 caatgatccc catgatgata gcggcgcctg atacgagcaa ctgctcgcac gccagcatcc
     8461 ctatcacttt ggcaagcgac aggcccatag ccctcagtat accaaactgg agcgtcctgt
     8521 tctggatgga tatgatccaa tagatcagga atcccagcag gcttatgagc atcgaggcta
     8581 tgaacccgag ggtaagcatt ccgttcattc cctggaccat ggcgtcattt ttcatcttta
     8641 taagttcctg gtccgtatat tttatgaatg acagttcaag gtcctttttg atgatgtcgt
     8701 cgtttatctg tttgtcggta gcgccgtcgg cctttttgat ccagacctca tacggctgga
     8761 gcgccatttt gttctgtata tatgaatagt tgacgaccac gagcccggcc tctcctccaa
     8821 ggtttctgtc agccttggga ttataggtgg gccagtagtc tatgaacgca tatatggtgc
     8881 cttccaggta gctttggctt ccccacgtga catatattgt atctccaagc tccagcttgt
     8941 atttatcctt caggttggat gatacgagga acgccgtggg agcttccgac atcaggttaa
     9001 tgtaatcatt aatgtggtac ggcagcagat cacgcctgaa ccaggcaacc tgtgcaaatt
     9061 cgtgcggtat tatgcccatt accttgagcc cgctggccac tcccatatcc gtctgcatag
     9121 taccgttatc gacattgagc accttggtca ccacttctgc tcctgaaagc gtcgcataat
     9181 cgttgaagtt cggttcatag tagcgaagcg gctcatctga ctgcatctgt gcctgcatct
     9241 gcgccatggt aggcgattgc gcaccttgcg gcgcattctt ctggttcgtt ttccatatcg
     9301 cctgaagccg tacgtctgct ccgacggaat accggatctt gtcctccata ttcttgttga
     9361 ccgtccttgc cgtattcgca ttgaaaagtc ctatcgaaat ggcaagtatc atgaaaagca
     9421 tcaggaactg ctcctgtcct ctcgatcttc cgacctgtat gagcgagatg tatgtgacag
     9481 gagtccatac cttcctgcca agccagtaaa tcagcctgat caggtacgga taaagccgta
     9541 tgaacagcag tcccgcgcca agtacaaagg ctgtcgatgt caggaacagc agaggatcta
     9601 tctggagttc attggctgag atacctgtcg cctcaagcac ccgctgctgc tgccggtatc
     9661 tgtagatacc atatccggca atcaaaagca gcactatatc aaggcccatc tttttccaaa
     9721 gggctgcctt cctggcgcgc gctttttcct gtttatggct tacgatagtt ttccgtgaga
     9781 ctgaaataac gggagcgagc atggtcacta tgaggaaaag tacggcaaac agcgaataca
     9841 tgtatacttt ggggctcaac tggaccggaa gggcggtcct ttgcacaaac tcgagaaagc
     9901 cattggatgc gccgagtatc ttgcatatga gcaagccgag gaaaggcccg gccaggtaag
     9961 ccacagcggc gacgatggaa ctttcgatca ggtagcttag gaatatctgc cccttcctcg
    10021 cgcctctgct tttgaacact gcgatctcgt tttcatcgtg atcgatgatc agctgggata
    10081 ccatgaagat gtagaaagtc agcatgatga ggacaggtac cgtaagtacc caaagcatgt
    10141 tctgaagctg tcttgccctt tcattgtact ttgccagtat cttgtttatc ggcatttcga
    10201 actcgatgct gactttatat ttagaaaacc acttttcctg ctcttcatat gtcctgacta
    10261 cgtgggccag gttctgcaga gtgatcgcat gataatcaaa tgcgcagtac cattccgctt
    10321 ccgtgagcgg tcctgttttt ggggcgataa atacatcgtt gaacagcccg tagtccatca
    10381 tgaattcctt attatgcatt gaaaactcat ggagccagta gacgtctccc gggtctttca
    10441 tctcgtatat accgacgacc ttcactttgc atacttcagc atagtctctt gccgggtcga
    10501 aaagcgtata ttccctgtca agcagaaggt ccaggatcac cattgcctgc tgcgacacaa
    10561 tgacctcgat ggccccgtct tcggtgatct gatccgaata catcctccct ttggttatag
    10621 ttatgtggtc ctcaaggccc gaaagggcac ccagcctgga gtaaaccgtt ttggtcctgt
    10681 aatcgtattc actggcattt tcaacaggcc ttgggaaaaa gctcacgctg cccaacacca
    10741 catggtgtga ggcactcttt atcggaaggc cgaaatcagt tatcttattg gtcattatct
    10801 ccttgttgaa ataacgctga ttactgatcc tctccgagta atcgttttta taagtgaacc
    10861 ttgcccttac cgaatattgt ccgggaaaat ccccggtttc cagctggtaa ttctccaggt
    10921 ccttggtcag cagtctctgc attatgccat ccgtatagat agggatgcaa ctgaccagtg
    10981 ccgtggccat aatggatccc aaaagcaggc atatgatcag ccatgagttg ctgaacatcc
    11041 ttctgagcac tatcttcagc aaatcctgcg cctccattct gttttttcag gtacatgcgg
    11101 ctcctgatac cgcttgcatt atcccgctgt tgcctcatcc cgcaaccggt ttatttcact
    11161 atcaggagtt ctccttcctg aaggcctttt actacttcaa cttccgtact ggtctgtatt
    11221 ccgacctcga tatcgcgttc ttccctgacg ccgtcattca gcacatttac aaacttcctg
    11281 tttgcaaacg tattaaccag cagtttcgga atgactatta cattttcctt tctctcgagc
    11341 gtaagcaaaa tggatgccgg ctgacccatc ctgatttcac cgggaagccc gtatacctcg
    11401 atctgtacgg attttttcgc ttcctcgtcc gcatcgtcgg gcgcctcgta aggagtcatg
    11461 acgatctccg catcatatgt ttcattgttg atctctacag cgacatccat accgatcctg
    11521 aagcttgtcg acctgtctcc ggaatacctg agctgaagtt ttgtcggatc ggcgaccctt
    11581 acgatggttt gataggcatt cacatagtct ccttccttga cactggctac atacaccaca
    11641 gtgccgtcga tcggcgagac aagcctggct ttctgaagct gggatttaag attttcgagc
    11701 ttgaccctgt tagattcaag gtccagttcc gccaggtcaa gtgacgtcct gctcccgccc
    11761 actatctcat agttggcctt tgcctcttcg tacaggatct gggatcgctc aagggcgatc
    11821 tcctggagtt ttatctcatt catcagggac tcggtatcaa gctccgccag gagatctcct
    11881 tttttgacct gctggccgga agagacatat atccccttga gcctgtccga ttttacgaaa
    11941 taaacatttt tctgactgac agaaacgaaa ttgcccgtga cccttatcac attttcaatc
    12001 gtgccccttg tgacctccac ggtctcgtat gtgatatttg caggcacttt gatcggcggg
    12061 gcaagttcct cctcctcttt cggaaagaag taacagcctg taagcgtcat cacagacaat
    12121 gccgccataa gcaagatcag gatacattta gacatgatcg ttccggaacg tttcttttct
    12181 gaattgtaca atgccaacac ctcgcgcact agtgatggaa aaagccatta tttatacaac
    12241 agtttatttt aacatttcct tcgccaataa ggtagttgtt tattttaagg ttaatggtgt
    12301 tttttttatg gaaatgtgtt atgtttgctt ttcattatac aatttaataa aatattcgta
    12361 atgaggtgct cataatgaac ctgtttcctt cccgcaacgg caaaacaccc caaaagtcgc
    12421 ccggcatcag ccgtctgtat atgcggctta aatcgcgcat cactctttat ttcttcatta
    12481 tggtcattct gccgtcgata gcggccgccc tggccatata tatgcataca aggtcgctca
    12541 tggtacaggc gtcatatgaa gcagctctga caaggatcac aaaggaaaac aactacatag
    12601 gttattgcct taaggaactg gaactggctg tagactcatc ggtgaattcc ataagtccac
    12661 aacagttgat acaattgcgt gaaggatcca atcacatcag taacgaaggc atactggccg
    12721 acatggccga caaatgcctg tcgccggcac agaggaaatc cctgtccgcg atatacgtgg
    12781 ccgattctga aggtatcagg gcttcttacg gacctgatgc ggacaaggca gtcatcagcg
    12841 gtttgtcttc ccagaagtgg ttcggggatg ccctggtcaa cggcaggaaa gtcctcgacg
    12901 gcattgactg gaccagcgcg ggcaattata ttgtt
//
LOCUS       DAOSCC010000041        12680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3335_length_12680_cov_5.225109, whole genome shotgun sequence.
ACCESSION   DAOSCC010000041 DAOSCC010000000
VERSION     DAOSCC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3335_length_12680_cov_5.225109"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1966)
                     /gene="glgB"
                     /locus_tag="PLG72_03655"
     CDS             complement(59..1966)
                     /gene="glgB"
                     /locus_tag="PLG72_03655"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOJ79926.1"
                     /translation="MDISAGFITEYDLYLFNEGSHHKIYEKLGAHPMTVNGRGGTHFA
                     VWAPNAKGVSLVCNLNRWDGRAHPLKPMGSSGVWAAFFPDIHPGEVYKYEIRTQSGDI
                     ILKADPYAFCSELRPGTASIVCGLDTYEWHDDEWIKSRDAGNTFNKPVSIYEVHLGSW
                     ARADGNRFLTYREFADRLVSYVKDMGYTHIELLPIAEHPLDDSWGYQVTGYYSVTSRY
                     GRPEDFMYFVDQCHMSGIGVIVDWVPAHFPKDAHGLARFDGTALYEHLDPRLGEHPDW
                     GTYIFNYTRNEVKSFLISNAVFWFDKYHIDGIRVDAVASMLYLDYGRKEGEWIPNEYG
                     GNQNIGAVEFLKQLNSAVFRYFPGIMMIAEESTSWPMITKPPYTGGLGFSYKWNMGWM
                     NDYLRYVSMDPVYRKYHHELLTFSIMYAMSENFVLVLSHDEVVHGKRSMIGKMPGDYW
                     QKFAGLRVTYGYMFGHPGKKLMFMGNEFGQFIEWNFSQSLDWHLLDYEMHRRLRDYVR
                     DLNRLYRDEKAMHEIDFSYDGFEWINCNDRDNSIVSFLRKGTRPEDTLVFVCNFTPVV
                     RSNYRIGVPYNTFYREILNSDSEIYGGSNAGNFGGIRANAEGHDGRPYSVDICVPPLA
                     VVVFKPVLQST"
     gene            complement(2143..2340)
                     /locus_tag="PLG72_03660"
     CDS             complement(2143..2340)
                     /locus_tag="PLG72_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOJ79927.1"
                     /translation="MDRWQYQTVQFDTTGFMGGIIDLDEFQATLNSLGADGWELVTCF
                     DTNMAQGQSRYVIAVFKRKIA"
     gene            complement(2455..3264)
                     /locus_tag="PLG72_03665"
     CDS             complement(2455..3264)
                     /locus_tag="PLG72_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ79928.1"
                     /translation="MDPYLYVPDTHEGFEILHVISGSGHILINNRIYEMLPNSIYFIN
                     GARFHYSNPIDPDNYVRNKVHFSQPEIIKFLQSIEQAQLLDPFLKDDSSYTCIRLTEE
                     SNLAIDQIFLEMHKESSEKKLGYRPIIAGLMLKILSLALRDRTDTSREEMQSLDKVSS
                     NAIKVIEYIENNLADFDLDKMADSLYLNKYYLCHMFKKVTGLTVHKFLLTKRIEQAKI
                     LLSSTDKPISQIAMELGFGSFSLFSSNFRRICSLTPREYRKMHRSGTVLHA"
     gene            3635..3898
                     /locus_tag="PLG72_03670"
     CDS             3635..3898
                     /locus_tag="PLG72_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79929.1"
                     /translation="MAQRLEKVDRLLGFLEENLDEKHCVEVLQRHMDAISGISGRETP
                     LSVYCKADHELYPITEVYNDPEKMLYNELVQAGEFGSMLNSAR"
     gene            4055..4162
                     /locus_tag="PLG72_03675"
                     /pseudo
     CDS             4055..4162
                     /locus_tag="PLG72_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            4191..5057
                     /locus_tag="PLG72_03680"
     CDS             4191..5057
                     /locus_tag="PLG72_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ79930.1"
                     /translation="MSQPIHPSLQQRHFVAQGFYKIFREKALKGEVYTAKESILFTTT
                     ALPGVISMNSTRVHFDPTDAKQRTQAEIEGHRQAASITEFLIKYVPGFEKAYLMDSGI
                     EVGFRESRHIVGEYTLQAEDVQRGRHFDDVIARYGFPCDVHKETKGSYFDDKDKSNKT
                     VEGLWIENDDAYDLPYRCLIPKTLNGLIVIGRCISVSHIAHGSTRLMPLAMAEGQAAG
                     IAAKLAIDSNVDVREVDIRKLQKRLIEEGASLYRDPEAVRIEKERARNAIKEFLANYE
                     SINTPEDVEWFD"
     gene            5100..5879
                     /locus_tag="PLG72_03685"
     CDS             5100..5879
                     /locus_tag="PLG72_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOJ79931.1"
                     /translation="MSLLKQKLLSGKLLIGTHVNLNDLVISEIMGSLGFDFLWIDTEH
                     TAIDYQCLLGHLVASKASGTPAIVRVPWNNAVFVKRVLEMGPAGIVFPMVNTAKEAEA
                     AMRCCIYPPTGNRGFGPNRAIRYGIDDVRKYINYDSMEICRFVQVETKEAVYNLDEIA
                     RNPYIDAFIIGPYDLSGSVGQLGNVMEGETDRLIDIAIEKAHQAGKPVGVSTGDNDYA
                     TLKHWLDKGIDIISTGTKYLGIIQGARTSLNNIKKIMEEKS"
     gene            6004..6453
                     /locus_tag="PLG72_03690"
     CDS             6004..6453
                     /locus_tag="PLG72_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOJ79932.1"
                     /translation="MIVDKLKNADLYYCLGEKMKKAFIYLQENDLSRLDEGRHSIDGE
                     DLYILVMRYDSKPVDQGKWEAHRKYIDIQYIVDGKEKMGYANIEEMKIVQEYDESNDI
                     LFADGKGSFIDTDKGTFVVFTPNDAHMPGVMDNIPQKVSKVVVKVRI"
     gene            6832..7524
                     /locus_tag="PLG72_03695"
     CDS             6832..7524
                     /locus_tag="PLG72_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79933.1"
                     /translation="MIYSHEVEKMTCVAKGPNHGPAPIPEEGKWVQAKEIKDISGLTH
                     GVGWCAPQQGACKLTLNVKDGVIQEALVETVGCSGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIAYGRSQTAFSEGGLPIGAGLEDLGKGLRSQVGTMYGTLAK
                     GPRYLEMAEGYVTRLALDDNNEIIGYEFVHLGKMMEMVRKGMDANEAIKKATNRYGRF
                     DEGVKFIDPREE"
     gene            7544..8545
                     /locus_tag="PLG72_03700"
     CDS             7544..8545
                     /locus_tag="PLG72_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOJ79934.1"
                     /translation="MALFEGYERRINQVNAALNKYGIASIEEAKKICDEKGIDVYSIV
                     RGIQPICFENACWAYTVGAAIAIKKGCKKAADAAEAIGEGLQSFCIPGSVADDRKVGL
                     GHGRLGAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANKVRKEPLRVILNGLGKDA
                     AQIISRINGFTYVQTKFDYFTGKLEIVREIPYSKGERAKVRCYGADDVREGVAIMHHE
                     KVDISITGNSTNPTRFQHPVAGTYKKECNELGRKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVSVAVAVEEAMNR"
     gene            complement(9011..10069)
                     /locus_tag="PLG72_03705"
     CDS             complement(9011..10069)
                     /locus_tag="PLG72_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOJ79935.1"
                     /translation="MYPLKFKPLYKDYVWGGRNLASLGKPLPDDGKVAESWEIACHKN
                     GSSIVANGEYEGTPLPELIKLLGRKLIGEALPQQDVEKFPLLIKLIDAQENLSVQVHP
                     NDEYAYANENGEYGKTEMWYIISAKPGAKLVYDVVPGTTRESFAEAVARNEVERCLNT
                     IEVSAGDVIYIPAGMVHAIGRGIIIAEIQQNSDTTYRVYDYGRVGRELHIQKALDVIN
                     FSSAGTGKKCTGITLPLSGGCTGRIVAANRYFCTEIYDIGGTAEEIADGSKFHIYVFT
                     SGTGVITWDAGQLTVRAGESVLIPAAMGKYSLEGNMTALKTFKPDLWKDVIRPLTEAG
                     FAEETIFSEIAGLRQAVS"
     gene            10276..11013
                     /locus_tag="PLG72_03710"
     CDS             10276..11013
                     /locus_tag="PLG72_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79936.1"
                     /translation="MENIAVEVRNLTKKYGRNTALQDLSFDIGKGEIFGLFGPNGAGK
                     STFLSILATILKPTSGDIRVNGYSVREQPGKVKGEIGYVPQDIALYPMLSGRENLDFW
                     AGIYGLRGKLKKERIEEAVEVVRLGDRIRDRVSRYSGGMKRRLNIAVSLMHHPRVLLM
                     DEPTVGVDIQSRSFILDALSALKKEGRTIIFTSHYIDELETLCDRIAIMDKGKILAMG
                     PADELKKANGADDLETVLLKLSGENAG"
     gene            11285..>12680
                     /locus_tag="PLG72_03715"
     CDS             11285..>12680
                     /locus_tag="PLG72_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79937.1"
                     /translation="MNTRTRKMILAAAIIVFAAAVIYSIWLALTRKNTAELYLEAEKK
                     EFAKFVDKIEEKYTSMKNKYQPYMEQAYSSRTELSLKVPGGLESFGFSDADTVRDILD
                     KTKLIVKTRNDPVKGISDTEANLLLERAPFLNARLYSDPRTIWLSIPDFLPDRYFSVQ
                     RSDLEGLYDRFSIPVKPLKLISGKEIAASLSFDGKSIRESADKLADIWAGYLTDDTVI
                     DNGTENVKFGADGQETEGSEVLIKLDEEKATSLLKELLTAVADDDVLLAYTYGNFANL
                     SELADDAGLFRLFRYLDETGAATLSDYEREILAKLNVRKDIEGFRESLKKTAQSYRLR
                     NGIEMRVVLDKEGDIIYRAVSLDFISNTGGKSFRADISTTGGDTGSRLFELILAEYDM
                     GSMSDIKRLTELNVDSRLNETKDSQKEGEINIRYVITEGSEKTGIDIGIDVSDKTDNQ
                     TMKRIRSINAEADIS"
BASE COUNT         3426 a         2949 c         3336 g         2969 t
ORIGIN      
        1 agggactgac aaggacgcgc tgatgacccg cgagagcttc gagagactcg aggagatatt
       61 aggttgactg gaggaccggc ttgaatacaa cgactgccag cgggggcacg caaatatcga
      121 cagaataagg ccgtccgtca tgtccttctg cattcgcccg gatgccgccg aaattgcccg
      181 cattgctgcc gccataaatc tccgaatcgc tgttcagtat ttccctgtag aatgtattgt
      241 aaggaacgcc tatcctgtag ttgctgcgga cgacgggcgt gaaattgcac acgaatacca
      301 gcgtatcctc cggccttgtt cctttgcgga gaaaggacac tatgctgttg tcccggtcgt
      361 tgcagttgat ccactcgaac ccgtcgtaac tgaaatctat ctcgtgcatt gccttttcgt
      421 ccctgtacag cctgttcagg tcgcgcacat aatctctgag cctgcggtgc atctcataat
      481 ccagcaggtg ccagtcaagg ctctggctga aattccattc aatgaactgg ccgaactcat
      541 tgcccatgaa catcagcttt tttcccgggt ggccgaacat atatccgtag gtcaccctga
      601 gcccggcgaa tttctgccaa taatcccccg gcattttgcc gatcatcgag cgtttgccgt
      661 gcactacctc gtcgtgggac agcaccagca cgaaattttc gctcattgcg tacataatgg
      721 agaaggtcag cagttcgtgg tggtatttcc ggtagaccgg atccatgctt acgtacctca
      781 ggtagtcgtt catccagccc atgttccatt tatatgaaaa tcccaggccg cctgtatatg
      841 gcggttttgt gatcatcggc cacgaggtgg actcttctgc gatcatcatt atccccggga
      901 aatatctgaa tactgccgag ttgagctgtt tcagaaattc aacggcgccg atattctggt
      961 ttccgccgta ttcgttgggg atccattcgc cctctttcct tccatagtcc aggtagagca
     1021 tggaagccac ggcatccacc ctgataccgt ctatatggta cttgtcgaac cagaatacag
     1081 cattggaaat aagaaagctt ttgacctcgt tgcgggtgta gttgaatatg taagtgcccc
     1141 agtcggggtg ttcgcccagg cgcggatcca ggtgctcata cagcgccgtg ccgtcgaacc
     1201 ttgcaagccc gtgtgcgtcc ttcgggaaat gtgcaggcac ccagtcgacg atcacgccga
     1261 tcccgctcat gtggcactgg tcgacaaagt acatgaaatc ctcgggccgt ccgtatcggc
     1321 tggtgacgga atagtagccg gtgacctggt agccccatga atcgtcgaga ggatgttcgg
     1381 caatcggcag cagttctata tgggtatagc ccatatcttt tacatatgac accagcctgt
     1441 cggcgaattc cctgtatgtc aggaaacggt tgccgtccgc gcgcgcccat gaaccgagat
     1501 gcacctcgta tattgacacc ggtttgttga atgtatttcc ggcgtccctg ctttttatcc
     1561 attcatcatc atgccattcg tacgtgtcga gtccgcagac tatagaagcc gtacccggcc
     1621 ggagttcgct gcaaaaagcg taagggtcgg ctttaaggat tatatcgcca gattgtgtcc
     1681 gtatttcgta cttgtacact tcgcccggat ggatatcagg aaagaacgca gcccacactc
     1741 ctgagctccc catcggcttc agcggatggg cacgcccgtc ccagcggttg aggttgcata
     1801 ccaggctgac gcctttcgcg ttgggagccc agaccgcgaa gtgggtcccg ccccggccgt
     1861 ttaccgtcat ggggtgggcg ccgagctttt cgtatatttt gtgatggctg ccttcgttga
     1921 acaagtacag gtcatattcc gtaatgaacc cagccgatat gtccataccg tcatgactat
     1981 tttcctggtt atgtacctga tgatccatcc tttcgattct ccctgccatg tatttgcagt
     2041 tccgcccggg cggttttccg cggtccatgc gcagtctgac ccgtgtcaga actctggcgt
     2101 tcatataccg cactgcccgg aacgaacttc tgccgtaatg tatcaggcaa tttttctttt
     2161 gaacaccgcg atcacatacc tggactggcc ctgtgccata ttggtgtcaa aacaggtaac
     2221 cagttcccag ccgtctgctc ccaggctgtt cagcgtggcc tggaattcat ccagatcgat
     2281 gatcccgccc atgaagcctg ttgtatcgaa ctgaactgtc tgatactgcc acctgtccat
     2341 agaaacatca ccttttactt ccggcagcgg aaagaccgct gttttatata tcattatact
     2401 acgaatcctt ggaattttct tcagccggac gaacggttat ttcagtctgg ttgcctatgc
     2461 gtgaagtact gtgccggagc ggtgcatttt gcggtattcc ctgggagtca ggctgcagat
     2521 cctgcggaaa ttgctgctga acagtgagaa gcttccaaat cccagttcca ttgctatttg
     2581 cgatataggc ttatctgtgg atgacaggag tatttttgcc tgctctatcc tttttgtcag
     2641 caggaattta tggactgtca gcccggtgac cttcttgaac atatggcaca ggtaatattt
     2701 attcaaataa agcgagtctg ccattttgtc caggtcgaaa tccgccaggt tattctcgat
     2761 atattcgatg acctttatcg cgttcgatga gaccttatcg aggctttgca tctcttccct
     2821 gcttgtatcc gtcctgtccc gaagcgccag agacaggatc ttcagcatga gtcctgctat
     2881 aatgggcctg tatcctagct tcttctcact cgattcctta tgcatctcaa gaaaaatctg
     2941 atcaatagcg aggttggact cttcagtcag ccgtatgcat gtatagcttg agtcatcttt
     3001 taaaaaagga tcgagaagct gcgcctgttc gatactctgc agaaatttga tgatttccgg
     3061 ctgactgaaa tggaccttgt ttctcacata gttatcagga tctatagggt tggaatagtg
     3121 gaaacgtgct ccgtttatga aataaatgct gtttggcagc atctcataaa tgcggttgtt
     3181 gatcagtata tggcccgaac cgcttatgac gtgcaggatc tcaaatcctt catgagtgtc
     3241 ggggacatac agatacggat ccatccctgt ataatgataa cttattccat caaagtatat
     3301 actgctcaaa ttatttagca attgctgcaa cacctctgct ttgcggagta tatcccctat
     3361 tgtccatatg gcgcggtttc ggtgcaaaag tatttatata tatgataata acatcattcg
     3421 gcagaataaa caataacggc ggcgatgcgg cgccacccgt tgggcacaat taccaaagac
     3481 cgttgattta tgtaaacagc aatattttga taatttcaag caagatatca gtatttatga
     3541 catacgaatg gccttaaaat ataatcgtag ggtaaaggaa tcggcagtat caccggacgc
     3601 ggcattgctt ttcccagaca ggatggtgga tctgatggct caacgattgg agaaggtgga
     3661 ccgtttactt ggattccttg aggaaaacct cgatgagaag cactgcgtcg aagtattgca
     3721 gcgccacatg gatgccatca gcgggataag cggacgcgaa acgccgttgt ccgtgtactg
     3781 caaagcggac catgaactgt acccgatcac ggaagtttat aacgaccctg agaagatgct
     3841 ttacaatgag ctcgtacagg ccggagaatt cggaagcatg ctgaattcgg ctcgatgagg
     3901 tcgtagcacg ctataagaaa taacgggaca gtatcataac cggtttgaaa agtattgatt
     3961 cgtaacagaa aagtgccatg gagggtgcgc cgggcggaag ctgcagcgct ccctcctgga
     4021 aaatacaact gaagtaatga cgggaggttt gtgtatgcat tctgagaact atgacgtaat
     4081 agttgtcggt ggaggtacaa gtggcgtaat agcagctatt gctgccggaa gagaaggcgc
     4141 caggacattg ctgatcgagc ggtacggatg taagaaccga acagataccg gtgagccagc
     4201 ccatacatcc ctcgctgcag cagcggcatt tcgtcgcaca gggattctac aagatattca
     4261 gggaaaaagc gctgaaaggc gaggtatata ccgcaaagga aagtatactg ttcaccacga
     4321 cggctctccc tggcgtaata agcatgaatt cgacacgtgt ccatttcgat cctacggatg
     4381 caaaacaaag gacacaggcg gaaatcgaag ggcacaggca ggctgcaagc attaccgaat
     4441 tccttataaa gtacgtaccg ggatttgaaa aggcatacct gatggattcc gggatcgagg
     4501 tcggtttccg tgaatcgcgc catatcgtcg gagaatatac tctgcaggct gaagatgtac
     4561 agcgcggaag acactttgac gatgtcatcg caaggtacgg gttcccgtgt gacgtccata
     4621 aggaaaccaa aggatcatat tttgacgata aagacaagtc gaacaaaacg gtcgaaggtc
     4681 tgtggatcga aaatgacgat gcttacgatc ttccttatcg gtgcctgata ccaaagactc
     4741 tgaacggtct catagttatc ggcaggtgca tatccgtatc ccatatcgcg cacggcagca
     4801 cccgcctgat gccattggcg atggccgaag gccaggcggc cggaatcgca gcaaaactgg
     4861 cgattgacag caatgtcgat gtacgagaag tcgatatcag gaagctccag aagcgtctca
     4921 tcgaagaagg agcatcattg tacagggatc cggaggcagt caggatcgaa aaggaaaggg
     4981 cacgcaatgc tataaaagag ttccttgcca attacgaatc gatcaataca cccgaggatg
     5041 ttgagtggtt tgactgatgt aactgcagca agctcaaaac agcattgtag gaggacatta
     5101 tgagtttatt gaagcaaaag ctattgagcg gcaaattgct gatcggtacc catgtcaacc
     5161 tgaatgacct cgtgatcagt gaaataatgg ggagtctcgg cttcgatttc ctttggatcg
     5221 atactgaaca tacagccata gactatcaat gcctgctggg tcacctggta gcatccaaag
     5281 cgagcggaac gccggctatt gtgagggtgc cgtggaataa cgccgtattt gtaaaaagag
     5341 tattggaaat gggaccggca ggtatcgtct tcccaatggt caataccgcg aaagaagccg
     5401 aggccgcaat gcggtgctgc atctatccgc cgaccggcaa ccgggggttc gggccaaaca
     5461 gggcaatacg ctacggcata gacgacgtca ggaaatacat caattatgac agcatggaga
     5521 tatgcagatt tgttcaggtc gagaccaagg aagccgtata caacctggat gagatcgcac
     5581 gcaaccctta tatagatgcg ttcataatag ggccgtatga tttgagcggc tctgtcggac
     5641 agcttggcaa tgttatggaa ggtgagacag accgcctgat cgatattgcc atcgaaaaag
     5701 cccatcaagc gggaaaaccc gtaggcgttt cgaccggaga caacgattac gcgacactga
     5761 agcattggct ggacaagggg atagacatca tttccaccgg taccaagtac ctcggcatta
     5821 tacagggcgc aaggacttcg ctcaataata tcaaaaaaat aatggaagaa aaatcatgag
     5881 tcttatgcct gtttgactgg gaaaggcaga tgctttgaac ggtattcgtg atcgccgcgg
     5941 tacccggatc gtctgccttt ttcagaatca aacgggacac ggcatgcaga ccggaggttc
     6001 catatgatag tcgataaact taagaatgct gatctatatt actgccttgg cgagaaaatg
     6061 aagaaagctt ttatatattt gcaggaaaat gatctgagca ggcttgatga gggcaggcac
     6121 agcattgatg gcgaggatct gtatatactt gtcatgcggt atgatagcaa acctgtagat
     6181 caggggaaat gggaagccca caggaaatac atcgatatcc agtatatagt cgacggcaaa
     6241 gagaaaatgg gatacgcaaa tatcgaagaa atgaaaatag tccaggaata tgatgaaagt
     6301 aatgatatcc tgtttgcgga tggaaaggga agttttatag ataccgataa aggtacgttt
     6361 gttgtgttta ctcccaatga cgcccatatg ccgggcgtta tggataatat cccccaaaag
     6421 gtaagtaagg ttgttgtaaa agtacggatt tagctggatt cagatcagat gtgctgtgaa
     6481 tggatattgc atcattattg cttcgcgtaa tgatgacgga gtccattctt ttttttgctt
     6541 agaaaaacag atgcgtctgt gataatatga taaaaaatta acatatacaa tgaaacaata
     6601 ttgtagtaaa ataaaacttg gcatgtttgc cacgcttttc ttgtaatctg ccgaaaaacc
     6661 gacaaattgc ggaatgagcg gggaactgtg aaaaacggat tgcaaaaacg gaaatgaaaa
     6721 aggtaaacgt gtaaaaccgg agtatgcaat aagcgatata tgcaataaac cggtagaaga
     6781 agaccggctg ctggataaag ctctaaaacc aaatatgaaa ggggtcatag aatgatttat
     6841 tcacatgaag ttgaaaaaat gacgtgtgtg gcaaaaggac cgaaccatgg tccggcaccg
     6901 atccccgagg aagggaaatg ggttcaggcc aaagagatca aggatatttc aggtcttacg
     6961 catggagtgg gctggtgcgc gccccagcag ggtgcctgca agctcacgct gaatgtcaag
     7021 gacggggtaa tacaggaagc gctggtggag acggtcggct gttccggcat gacgcattcg
     7081 gctgcgatgg ctgctgagat actcccgggc aaaaccatac ttgaagcgtt gaacacggac
     7141 ctggtctgcg acgctatcaa cacggcaatg agggagctgt tcctgcagat cgcctacggc
     7201 aggagccaga ctgctttctc ggaaggagga cttcccattg gtgcgggcct cgaggatctt
     7261 ggaaaaggcc tgaggagcca ggttggaact atgtacggaa cgctggccaa gggccccagg
     7321 tacctggaaa tggccgaagg ctatgtgaca cgtctcgcac ttgatgacaa caatgagatc
     7381 attggctatg agttcgtaca tctcggcaag atgatggaaa tggtcagaaa aggcatggac
     7441 gccaacgaag ccatcaaaaa ggcgaccaac aggtacggca ggtttgatga aggcgtaaaa
     7501 ttcatcgatc cgagagaaga ataatcaggg gagggtcagg aacatggcat tgtttgaagg
     7561 ttatgaaaga aggataaatc aggttaatgc cgcactgaat aaatacggta tagcctcgat
     7621 cgaagaagcc aaaaaaatat gcgacgaaaa aggcatcgat gtatacagca tcgtcagggg
     7681 aatacagccg atctgctttg aaaatgcatg ctgggcttat acggtcggcg ctgccatagc
     7741 cataaagaaa ggatgcaaaa aggctgccga tgcagcagaa gcaatcggcg aaggacttca
     7801 gtccttctgc atcccgggat cggtagcaga cgacaggaaa gtcggccttg gccatggaag
     7861 actcggagcc atgctgcttc gtgaagaaac caaatgcttc gcattcctgg caggccatga
     7921 gtcctttgct gctgcagagg gtgcaatagg catcgcgaaa tccgcaaaca aggtaaggaa
     7981 agagcccctg agggtcatac tcaacggcct tggaaaggat gctgcccaga ttatctcgag
     8041 gatcaacggc tttacatatg tgcagaccaa gttcgattac ttcacgggca agcttgaaat
     8101 agtcagggag atcccctact cgaagggtga aagggcgaag gtaagatgct acggcgccga
     8161 tgatgtacgc gaaggcgttg cgataatgca ccatgagaaa gttgacattt ctattaccgg
     8221 taactccacc aacccgacaa ggttccagca tcctgtcgca ggcacataca agaaggaatg
     8281 caacgaactg ggcaggaagt acttctcagt cgcttcgggc ggcggcacgg gaaggacgct
     8341 gcatccggac aatatggcgg caggacctgc ttcctacggc atgaccgata ccatgggcag
     8401 gatgcattcc gatgcacagt tcgccgggtc ttcttcagta cctgctcacg ttgagatgat
     8461 gggactcatc ggcatgggca acaacccgat ggtcggagca accgtttcag tggcagttgc
     8521 cgtcgaagag gcaatgaaca ggtaactgcc tgtggccgga agaccgtaca gatcaataag
     8581 tccagacaga atagtaaaag agccgttctg tgaaagcaca ggacggcttt tgtttattct
     8641 cacgggcaaa ttgcccatcc tgcaggtgcg ttcgggcatt aacagcttcc tgtttcccat
     8701 ggcagcagcg gcatcggaca gttaaacttc attcggtgac ggcttactgt tatttccccg
     8761 ggaaataaga accagggtgt acaaaaattg aattgttgtg ggagcaggat agagcttgat
     8821 ggcttatttt ataaacttaa gttgccataa tgcagggata cccgtattat ggcaactttc
     8881 ttattgataa ttcacaatat ttaagccatt tcaacacaca acaattaaaa ttttgtacac
     8941 ccaagtcatt atttcccagg gaaataaggc gggcagatat agctgctctt tcacccctgt
     9001 acagcttgcc tcaggacaca gcctgcctga ggcctgcgat ttccgaaaaa atcgtttctt
     9061 ccgcgaatcc cgcttccgtc agcggccgta tgacatcctt ccaaagatcc ggcttgaatg
     9121 tcttcaatgc ggtcatattg ccttccagtg agtatttgcc catggcggca ggaatcagca
     9181 cggattcacc tgccctgacg gtcagttggc ctgcgtccca tgtgatgact cccgttccgg
     9241 atgtgaacac atatatgtgg aatttgctgc cgtcggcaat ctcttcggcc gttccgccga
     9301 tatcgtatat ttcggtgcag aaatagcggt tcgccgctac gatcctcccg gtgcagccgc
     9361 cggagagcgg aagcgttatg ccggtacact ttttcccggt gcctgcagaa ctgaagttta
     9421 ttacatcaag ggctttctgt atatgcagtt cccttccgac gcgcccgtaa tcatacaccc
     9481 tgtaagtcgt gtccgagttc tgctggatct ctgcgatgat gatgcccctg cctatcgcat
     9541 ggaccatgcc cgcggggatg tagatgacgt ctccggcaga cacttcaatg gtattgagac
     9601 agcgctccac ttcattcctg gcgacagctt ccgcgaagct ttcgcgggtg gtgcccggca
     9661 ctacgtcata cacgagcttt gcgccgggtt ttgcgctgat tatgtaccac atttctgttt
     9721 tgccatattc gccgttctca ttcgcatatg catactcgtc gttaggatgc acctgcacgg
     9781 agagattttc ctgtgcatcg atgagcttta taagcagggg aaatttttca acgtcctgct
     9841 gcggcagcgc ttcgccgatc agtttcctgc caagcagctt tatcagttcg ggaaggggtg
     9901 ttccctcata ctccccgtta gccacgatac tggagccgtt cttatggcac gctatttccc
     9961 agctctcagc cactttgccg tcgtcaggca ggggtttgcc cagcgacgcc aggttcctgc
    10021 cgccccatac ataatcttta tacaacggtt tgaatttcag cggatacatg atttaagcct
    10081 ccggttaatt cgtattctgg caatatcgat caaggaatag tatatagtat cgggcatgcc
    10141 gtttcaatgt gatatccaga acaggtcctt gtatgataaa atattgtttg tataaacaga
    10201 taatcagaaa agcggacgac cggaccggga cgacgtcgat acaggcacgg gtgcggtcgg
    10261 gtcaagggta tgggtatgga gaacatagca gttgaggtaa ggaatctcac taaaaaatac
    10321 ggcaggaaca cggcgctgca ggatctgtcc ttcgatatcg gaaagggcga gatattcggc
    10381 ctgttcggac ccaacggcgc ggggaaatcc acgttcctgt cgatacttgc gacgatcctg
    10441 aagccgacgt cgggcgatat cagggtaaac gggtacagtg tacgtgaaca gcctgggaag
    10501 gtgaagggag agataggcta tgtgccgcag gatatcgcgc tgtatccgat gctttccggc
    10561 agggaaaacc ttgatttctg ggccggtatt tatggcctgc gcggaaagct caaaaaggaa
    10621 aggatcgaag aagccgtcga ggtcgtcagg ctcggagaca ggataaggga cagggtatcc
    10681 cggtactcgg gaggcatgaa aagaaggctg aacatcgccg tatcgcttat gcaccatccg
    10741 agggtcctgc tcatggacga gccgacggta ggcgtcgata tccagtcccg cagctttata
    10801 cttgacgcgc tttccgcatt gaaaaaggaa ggcaggacca tcatattcac cagccactat
    10861 atcgatgaac ttgaaacgct atgcgacagg atagcgatta tggacaaggg aaagatactt
    10921 gcgatgggac cggctgatga actgaaaaaa gccaatgggg cggatgacct ggaaacagtg
    10981 ctgctgaaac tgtcaggtga aaatgcaggc taagaaggta aagtacagta cagctaagta
    11041 cagtaaagta aggcatggca cggaaaaatc gaacactgta tagtaaagca cagtacaaga
    11101 aagtacacat tgtgcgtata gcgaagttac acatagtaca gtacggcgta acacaatata
    11161 gtacaacaga atacagcaca ggacagtata gcacatatta cagaatgtat cagcatatag
    11221 cgggattgat gccggataat tcaggtcgat accggataat gcaagataat caggagtgat
    11281 aacaatgaac acacgtacga gaaaaatgat ccttgctgca gcgatcatag tatttgctgc
    11341 agcggtcata tatagcatat ggttggcgct gacgcggaaa aacacggcgg aactgtacct
    11401 ggaagcggaa aagaaggaat ttgccaagtt tgttgacaaa atcgaagaaa agtacacttc
    11461 tatgaaaaac aaataccagc catacatgga acaggcatac agcagcagga cggaactgtc
    11521 cctgaaagtc cccggcgggc ttgaatcctt cgggttcagc gatgctgata ccgtcaggga
    11581 catcctggac aaaaccaagc tgatcgtaaa aaccaggaat gacccggtga aggggatctc
    11641 cgacacggaa gcgaatctgc tgcttgaaag ggcgcctttc ctgaacgccc ggctgtattc
    11701 ggacccccgt acaatatggc tgtcgatccc ggatttcctg ccggacaggt atttcagcgt
    11761 gcagcggagc gatcttgaag gcctttacga caggttctca atacctgtca aaccgctgaa
    11821 gctgatcagc ggaaaagaga tcgccgcaag cctttccttt gacggcaaat ccatcaggga
    11881 atccgccgac aaactggccg atatctgggc aggatacctc acggatgata cggtcatcga
    11941 caacggcacg gaaaatgtta aattcggcgc tgacggacag gagacagagg gcagcgaggt
    12001 gctgatcaaa ctggacgagg aaaaggcgac gtcgctgctg aaggaacttt tgacggctgt
    12061 ggctgatgac gacgtcctgc tggcatatac ttacggtaac tttgcaaatc tttccgaatt
    12121 ggcggatgat gcaggattgt tccggctgtt cagatacctt gacgaaaccg gcgcagcaac
    12181 ccttagcgac tatgaaaggg aaatactggc aaagctcaac gtacgcaagg atatcgaagg
    12241 gttcagggaa agcctgaaga agaccgcgca gtcataccgc ctcagaaacg gaatagaaat
    12301 gcgcgttgtg ctggacaagg aaggagatat aatctacagg gcggtcagtc tggatttcat
    12361 cagcaacaca ggcggcaaat cgttcagggc tgatatatcc acgaccggag gcgatacggg
    12421 atccaggctt tttgagctca ttttagcgga atatgacatg ggcagtatga gcgatataaa
    12481 gaggttgacc gaactgaatg tcgattcccg gctgaacgag acaaaagatt cccaaaaaga
    12541 aggggaaata aacatccgtt atgtcatcac cgagggcagc gaaaagaccg ggatagatat
    12601 cggcatcgac gtttcagata agaccgataa tcaaacgatg aagcggataa gaagcatcaa
    12661 tgcggaagcg gacatatccg
//
LOCUS       DAOSCC010000042        12613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3359_length_12613_cov_4.437888, whole genome shotgun sequence.
ACCESSION   DAOSCC010000042 DAOSCC010000000
VERSION     DAOSCC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_12613_cov_4.437888"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2878)
                     /locus_tag="PLG72_03720"
     CDS             complement(<1..2878)
                     /locus_tag="PLG72_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ79938.1"
                     /translation="MKKIAARKLSKLLAVALALVFAVSGTGISYGDTVASTDTGGTET
                     ISDSVIRRTDTYEYYISQYQDAEKPQVEVEIEVDRYIDADMDVEILENFEGSAGKSLK
                     TDEEGYVTWEADVPQEGLYSLYIEYFPIEGRSSSIEREVWINGESPFNDAKHITFSRV
                     WVNAEEVRRDNRDNDIRPKQEEDPHWLSSYFNDYMGYYNEPYLFYFKKGKNTLKLVSV
                     KEPMVIRTLKLTQYKTVPTYEEKLEEWREKGYEIYDGDSIIIQGESAVYKSDPTLYPL
                     NDRTSPSTVPYHKSKIRLNTIGGTNWQQPGQWIEWKVNVEKEGLYQITIKGRQNITRG
                     KYSCRRLLINGEVPFKEAENIPFIFSSSWQNFVLGNEDEPYLFYLKEGENTIKMEVVL
                     GDLADILRKAEESVYILNSAYRKILMIVGSSPDRYRDYQLHKKIPGVISTLDEQAELL
                     RSLTEDLVALTGQRGSNHAILNRLAYQLEDMAERPRTIQNRMTQFRDNVGALGTWIND
                     TRNQPFEIDYFIFSSPGAELPKAEASFFRRVFHEISAFIASFVEDYSSVGNVYDTDEA
                     ITVWITTGRDQAQVLKKIIDDSFVPETGIAVNLELVQANVLLPATVAGRGPDVALSVL
                     NSEPVNYATRNAAIDLTQFPDLDEVLKEFTDASLQPFRFQGGLYGLPEQQLFLMMFYR
                     KDILDELGLEPPETWEELYYIIPAIQKVNMEVSLPVAVNNGGNYDPWGAMESYSTFLY
                     QEGGQIYNGDGISSALDTEEAIRAFKKWSELYVNYKFPVIFDALNRFRTGQMPLMLHD
                     YWLYNNLQVFAPELRGLWEMAPIPGVMREDGTIDRSTIGGGYSAMILRQTKNKEAAWK
                     FLKWWVSADTQVRFGREMESLMGAAARHPTANIEAAKQIPWPVKDYNALTEQRKWVKG
                     NPEVPGGYFTSRHLSNAFRKVYNEGADPRETLLDYVRTINEEIDN"
     gene            3309..5063
                     /locus_tag="PLG72_03725"
     CDS             3309..5063
                     /locus_tag="PLG72_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOJ79939.1"
                     /translation="MPFDGVVAKSIVQELTGELAGGRIDKILQPESDEIVLVVRARSK
                     NSRLLLSASPRYPRIHLTETVKENPPVPPAFCMLLRKHLARGRIISFEFSDYERIIGI
                     NIEAAGELGDISQKKLVIEIMGRHSNIILLNEAGRILDAIKHVDSDISSVREVMPARL
                     YILPPPQDKVSPSRLDIPELVRLLGSSDKSIEKFLLDNIKGFSPLLCREVCWRADIDG
                     HGRASVLSDGERTALAQALRSVIEEIRNDEYHPAVYYEDEQHQVPLDFHCIGISQYAF
                     GELFDTVSQALDRFYASRDNAEHLRKKKADLYKVLNNSIERCSKKLAIQQDALRESAD
                     REKFRLYGELITANIYAIPGNAEKVSLMNYYSENGEYVEIPLDPDLTPQANAQHYFRK
                     YAKAKSTYINTTHQIQQTQIELDYLESVLQTLDNCTSVQEIDEVRQELADQGYMVRKR
                     RRSDKKETAATKPFRYRSSDGFDILVGKNNIQNDRLTLRTAQSGDIWLHTKAVPGSHV
                     IIRRNGRDIPDRTIEQAAIIAAWHSKARMSSNVQVDYTQVKNVSKPSGAKPGMVIYTN
                     YKTAVVTPDQSLVESLRV"
     gene            5069..5269
                     /locus_tag="PLG72_03730"
     CDS             5069..5269
                     /locus_tag="PLG72_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ79940.1"
                     /translation="MYRTAAVPVSEVVDTPGCGDSYIAAFCADYFRGGTIQSAMETGS
                     LAASRVLSRRGGAENGIMTVLR"
     gene            5386..5928
                     /locus_tag="PLG72_03735"
     CDS             5386..5928
                     /locus_tag="PLG72_03735"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOJ79941.1"
                     /translation="MERDDIEMANAAEKAGKYIRKAMDMGADHAVFFRTEDIAFDPRS
                     LLKCMFGCSSWGKDHTCPSRPGSLKPWEYEQIFRRYSWGIIIHSHSKKISQDISFAIE
                     SQAFTDGYYMAFSLSDCSICPECGGKRGMPCADPKKARPAFHSVGIDVFKTVRKFGLP
                     IETLKTESDEQNWYSAVFIE"
     gene            6109..7290
                     /locus_tag="PLG72_03740"
     CDS             6109..7290
                     /locus_tag="PLG72_03740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ79942.1"
                     /translation="MVSEKILANLKRSSWIRAMFEQGENLRKKFGADNVYDFSLGNPD
                     LEPPEIVRDILKKAVLSDTPKMHGYMSNAGYPDVRAAVAENLRKSKGVPVEACHVVME
                     CGAGGAMNVVLKTILNPGNEVIVLAPYFAEYLFYIDNHGGVPVIVDTDRETFQPDAQK
                     IYNVITPKTKAIIINSPNNPTGVIYSRSSLESISEVIEKRQEEFGNTICVISDEPYDK
                     IVYDGAAVPSVFEVFKNSVIVNSYSKSLSLPGERIGFAAVNPRIGDTAPLVDGLIFST
                     RTLGFVNAPALFQRILPGSLDAKVAVDAYKKRRDMLYEIVTKAGFKCLKPQGAFYLFP
                     RSPIEDDVKFCNEALKYNLIIVPGTGFGFKGFFRLAYCVDEKTIANSQKAFEALAAEY
                     K"
     gene            complement(7665..8300)
                     /locus_tag="PLG72_03745"
     CDS             complement(7665..8300)
                     /locus_tag="PLG72_03745"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOJ79943.1"
                     /translation="MTCVVFCGGKIGDYSHAEKYCRNAELVISADSGARHCRALGIVP
                     DIALGDFDSIDRSDYSRLEEAGTEILRFPVEKDMTDSELAVETAISRGCSRIILLGAA
                     GSRLDHSISNLFLLKKMHDANVEGIIADDKYEVRLVSGSIRLKREEGVFVTLLPFGGD
                     ASGVSTRGLYFPLDNAVLKTGSSLGVSNRFSEDEAYVEVKEGLLLVITAKD"
     gene            complement(8297..8962)
                     /gene="rpe"
                     /locus_tag="PLG72_03750"
     CDS             complement(8297..8962)
                     /gene="rpe"
                     /locus_tag="PLG72_03750"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ79944.1"
                     /translation="MIKIAPSILASDFSKLGEEISRVERAGADMIHLDVMDGHFVPNI
                     TIGPPVIKSLRKVTELPFDVHLMIEDPDRYIKDFADAGANIISVHVENNPHLHRTIEL
                     IRECGARPSVVLNPATPLNTLDAVLGDVDMVLLMTVDPGFGGQHYIENMTEKIRELRQ
                     IAVKRGLSLDIEVDGGIDLSNIWDVTQAGANVIVAGTTIFGAPDAAEIMSSLRQKAYG
                     GTI"
     gene            complement(8955..9878)
                     /gene="rsgA"
                     /locus_tag="PLG72_03755"
     CDS             complement(8955..9878)
                     /gene="rsgA"
                     /locus_tag="PLG72_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ79945.1"
                     /translation="MPSGIIIRGIAGFYYVAAKDDGVYECRARGIFRKKGLTPLTGDR
                     VVFSVTDPAGKKGNIEEITERSSVLVRPAVANVDQIIAVIAAKSPDPDLMLLDKILVT
                     AEDKGIRAVVCINKIDLDEGGKRARLREPYIKAGYEVIETSFRESDGFSKLKEALKGH
                     ISVFAGQSGVGKSTLLNRVMDTVLMKTGSVSEKIERGRHTTRHAELIELPGGGYLVDT
                     PGFSSFELTGIEYDRLQFLYPEFAEHIPKCRFKGCSHVFEADCAVKKAVDERSIEKGR
                     YARFAELYKTLKQEDEAKYKKGGRKGMKGND"
     gene            complement(9935..11929)
                     /gene="pknB"
                     /locus_tag="PLG72_03760"
     CDS             complement(9935..11929)
                     /gene="pknB"
                     /locus_tag="PLG72_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOJ79946.1"
                     /translation="MGNRYKLLERIGGGGMAVVYKAKCLLLNRFVAVKILRSEFTDDE
                     EFVKRFRVEAQAAASLNHPNIVSIYDVGKQDDIQYIVMELIDGITLKEYISQKGALPW
                     KEAVGIAIQICSALEHAHKNFIVHRDIKPHNIMITKDGIAKVTDFGIARAVTSATITM
                     VGSTIGSVHYFSPEQARGGFIDEKSDLYSLGITIYEMVTGKVPFDGESPVAIALKHIQ
                     EKPEKPIDINPKIPKGVNDLIIKAMKKDQNLRYQTASEMLADLQRVLADPDTKINGSN
                     AVEELPTKKMQAVGTGVDIIKENTDDGDEGYMEEDGEADNRKKRDWLSICLGIASGVV
                     IMALVVYLGIKLVIPMIAPDDTASFVVEDYTNMDFDEVKELLSQYDITAVDNERVFNE
                     TVKKGVIISQDIAVGNTLKPGSTINFTVSDGPHMVEIPDIRGQEYRIAEVEITNAGLV
                     PEIVRENNDKVGKDLVIKTEPEAFEMVKPDTVVKVYVSAGPKLEKVTVPDFGGMTRTE
                     AENAILKNNLTIGDFLPKDIVSDVAKVIKQSPLPNEVVDEYTAVDLTFGIDEHPEEGR
                     GGDAPSGPDDGGSQQVSTAKTKAISITLNPSRTYGEEVLVYVESTPSDTNVRKIEMNQ
                     YVRRDLFPFSIPITLGETGTTHVIVYLDNDLALETDIQNQ"
     gene            complement(11958..>12613)
                     /locus_tag="PLG72_03765"
     CDS             complement(11958..>12613)
                     /locus_tag="PLG72_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOJ79947.1"
                     /translation="GRSDTSCVFVIADGLGGHNSGEVASRMAVEYIRDNIGAEGCFDS
                     ADLSGKLEALVKKVNTAVYERSLGSAEVSGMGTTLTMAVIAGSTLTAAHVGDSRLYLI
                     RNGAIRQLTEDHSYIGELVRQGKLTREEAEKHPRKNIITRAIGSSPELEVDIISHQIE
                     ADDIYILCTDGLTNMVSESDIADIVSGSDPEHACAKLIEAANKQGGEDNITVIVIKC"
BASE COUNT         2969 a         3380 c         2944 g         3320 t
ORIGIN      
        1 tattgtctat ctcttcattt atcgtcctga cataatcaag cagagtctcc ctgggatccg
       61 cgccttcgtt gtatactttc ctgaaagcat tgctcaggtg tctcgaggtg aagtaaccgc
      121 ccgggacctc cgggttgccc ttgacccatt tcctctgctc cgtcagcgca ttgtaatcct
      181 tcaccggcca cggaatctgt ttagcagctt caatgtttgc agtcgggtgg cgggccgccg
      241 cacccatcag gctttccatc tcacgtccga acctgacctg agtatcggcg ctcacccacc
      301 atttcaggaa cttccatgca gcttccttgt tctttgtctg cctgagtatc atggctgagt
      361 atccgccgcc gatggtggac ctgtcgatgg tcccgtcctc tctcatcacg cccggtatgg
      421 gcgccatctc ccaaagtcct ctcagttcgg gagcaaatac ctgcagattg ttgtagagcc
      481 agtaatcatg gagcatcagc ggcatctggc ctgtcctgaa acggttcagc gcatcgaata
      541 tgacagggaa tttgtagttc acatacaact cgctccactt tttgaatgcc cttattgcct
      601 cttctgtatc gagagcgctt gagatgccgt ctccgttata aatctgtccc ccttcctggt
      661 aaaggaacgt actgtaggac tccattgctc cccacgggtc atagttcccg ccattgttca
      721 cagcgaccgg aagacttact tccatattga ctttctgtat ggccgggatg atatagtaaa
      781 gttcctccca ggtttccggc ggttccaggc ccagttcatc caggatatcc ttacggtaga
      841 acatcataag gaacaactgc tgttcaggca ggccatacaa gccgccctgg aacctgaaag
      901 gctggagcga tgcatctgtg aattccttga gcacctcatc gagatccggg aattgggtca
      961 gatctatagc agcattacgc gtcgcataat tgaccggctc gctgttcaaa acagacaagg
     1021 ccacatccgg ccccctgcct gcaactgtag caggcagcag cacatttgcc tggaccagtt
     1081 ccaggttgac ggctatgcct gtctcaggca cgaacgagtc atctattatt ttcttgagta
     1141 cctgggcctg gtcacgtcct gtggtgatcc agacagtgat ggcttcatcc gtgtcatata
     1201 cgtttccgac actgctgtaa tcttcgacga aagatgctat gaaggctgag atctcatgga
     1261 acacgcgtct gaagaaggat gcctccgctt tgggcaactc agcgccaggt gatgagaata
     1321 tgaaataatc gatctcgaaa ggctggttcc tcgtgtcgtt tatccatgta ccgagcgcac
     1381 ctacattgtc cctgaactgt gtcatcctgt tctggatcgt acgcggcctt tccgccatat
     1441 cttccaactg gtatgcaagc ctgttgagta tggcatgatt tgaaccgcgc tggcctgtca
     1501 gcgcgaccag gtcttcagta agactcctga gcagttcggc ctgttcatca agagtcgata
     1561 ttacgccagg gatcttctta tgcagttggt aatccctgta tcggtccggc gacgatccga
     1621 ctatcatgag gatcttcctg tacgcactgt tgaggatata gacgctttct tccgcttttc
     1681 tgagtatatc cgccagatct ccaaggacta cctccatctt tatcgtgttt tctccctctt
     1741 tcagataaaa caggtatggt tcgtcttcgt tcccgagtac gaagttttgc caggaggatg
     1801 aaaagatgaa cggtatgttc tccgcttcct tgaaagggac ctccccgttt atgagcagcc
     1861 ttctgcagga atacttgcct cgggttatat tctgtctgcc tttgatcgtt atctggtaaa
     1921 ggccctcttt ttccacattc actttccatt cgatccactg tcccggctgc tgccagttcg
     1981 tacccccgat cgtattcaga cgtatcttgg atttatgata tggaactgtg ctgggagacg
     2041 tacggtcatt gagcgggtac agtgtcgggt ctgacttgta aacggcgctt tcgccctgta
     2101 tgattatgga atcgccgtca tagatctcgt atcctttttc cctccactct tccagtttct
     2161 cttcgtatgt tgggactgtt ttatattgcg tgagtttcag ggtcctgatc accatgggtt
     2221 cctttacgga tacgagtttt aacgtattct tgcctttttt gaagtagaaa agataaggtt
     2281 cgttgtagta acccatataa tcattgaaat atgaagagag ccagtggggg tcttcttcct
     2341 gtttcggcct tatatcatta tcgcggttgt cccgcctgac ctcctccgca ttgacccaca
     2401 ccctggaaaa agtgatatgc ttggcgtcat tgaaaggact ttcaccattt atccagactt
     2461 cacgttctat ggatgagctg cggccttcaa tcgggaaata ctctatgtat agactgtaca
     2521 gtccttcctg gggcacatct gcttcccagg tgacatatcc ctcttcgtcg gtcttcagcg
     2581 atttcccggc cgaaccttca aaattttcga gtatctcgac atccatatcg gcgtcgatat
     2641 acctgtcgac ttcgatctca acttctacct gcggtttttc agcatcctgg tactgagaaa
     2701 tgtaatactc gtatgtatcg gtacgtcgga tcacggaatc gcttattgtt tccgtgcctc
     2761 ccgtatctgt tgaagcgact gtgtcgccat aagaaatgcc tgtacctgaa acagcaaata
     2821 ctaaagcaag cgccactgcc aatagcttag ataacttgcg tgcagctatc ttcttcatat
     2881 atacagcccc ttattgtgtt ttctttatat acaataaaaa cagtatagta tatgcttaac
     2941 aactgaaatt ttacagtgta aaaacaccag ggacaattga agttaaaatc aacaaaaaag
     3001 aaaacatgat cttttcataa tttgtatatg tttattatag ttttcctttc ctgttatgtc
     3061 aatcacaatt tgccggaaag cctgcgatta ttgacaatac agcattacag ggcataacgc
     3121 gtttacaggc atactttaca aaaacgttac acgggcttta catttttggc aagtttcaca
     3181 ttacgtatga caattcgtaa cgttacatat attcaaatca tatcgttcct ttatgatttt
     3241 ccttgtatcg gtttttgatt tttgctaaag tagaatcaat ctctgatgca agtaaaggag
     3301 tgttcctttt gccgtttgac ggagtcgtag caaaaagcat agtacaggaa ctgaccggag
     3361 aacttgccgg cggccgtata gataaaatac tgcagcccga atctgatgaa atagtgctgg
     3421 tggtaagggc tcggagcaaa aacagcaggc tgctgctgag cgccagtcca aggtatccca
     3481 ggatccacct taccgaaact gtcaaggaaa atcctcctgt tccccctgca ttctgcatgc
     3541 tgctgagaaa gcacctggcg aggggcagga taatttcatt cgagttcagc gactacgaaa
     3601 ggatcatcgg tataaacatc gaagctgccg gcgaacttgg agatatatcg caaaagaagc
     3661 tcgttataga gataatgggc aggcacagca acataatcct gctcaacgaa gcaggcagga
     3721 tacttgacgc tataaaacac gtagattcgg atatcagcag cgtccgggaa gtcatgcccg
     3781 cgaggctgta tatactgcct cctccgcagg acaaggtctc cccctcccgt ctggatattc
     3841 cggaactggt caggcttctc ggttcatcag ataaaagcat tgagaagttt ctgctcgata
     3901 atataaaagg tttcagtccg ctgttatgcc gcgaagtatg ctggcgtgcg gatatcgacg
     3961 gccatgggcg tgcatccgtt ctttcggacg gtgagcgcac cgccctggca caagcgctgc
     4021 ggtcggtgat cgaagagatc agaaacgacg aataccatcc cgctgtttat tatgaagatg
     4081 agcagcacca ggtgcccctg gatttccact gcatcggtat aagccagtat gccttcggag
     4141 agcttttcga caccgtcagc caggcgcttg acaggttcta cgcctcgcgt gacaatgccg
     4201 aacatctcag gaagaagaag gctgacctgt ataaagtgct gaacaactcc atagaacgat
     4261 gttcaaaaaa gctcgccata cagcaggatg ctttgaggga atcggcagac agggaaaaat
     4321 tcaggcttta cggcgaactg atcaccgcca atatctatgc gatacccggg aacgcggaaa
     4381 aagtttcgct gatgaattat tacagcgaaa atggagaata tgtcgagata cctcttgacc
     4441 ctgaccttac gcctcaggcg aacgcacagc attacttccg caaatatgcg aaagcaaaga
     4501 gcacctatat caacacgacg catcagatcc agcagacaca gatcgagctc gactacctgg
     4561 agagcgttct tcagacgctg gacaactgta catccgtgca ggagatcgac gaagtcaggc
     4621 aggaacttgc cgaccagggc tatatggtca ggaagcgcag gcggagcgac aaaaaggaaa
     4681 ccgccgcgac aaagccattc cgctacaggt cttcagacgg gttcgacata cttgttggaa
     4741 agaacaacat ccagaatgac cggctgactt taaggacggc gcagtccggc gacatatggc
     4801 tccatacgaa ggccgtcccc ggctcccatg taataataag gaggaacggc cgcgatatcc
     4861 ccgaccggac catcgagcag gcagccataa tagccgcatg gcacagcaag gccaggatgt
     4921 cttccaatgt ccaggtggac tacactcagg taaaaaatgt aagcaaacct tccggcgcaa
     4981 agccggggat ggtgatatat acaaactaca aaacagccgt tgtcacaccg gaccagtcgc
     5041 tggtggaaag cctcagggta taggtacaat atacaggacc gccgcagtgc cggtaagcga
     5101 agttgtcgac acccccggct gcggggattc ttatatcgct gcattctgtg ccgactattt
     5161 ccggggcgga actatacaaa gcgcaatgga aacaggctcg ctcgcggcat ccagggtcct
     5221 gtcacggcgc ggcggcgcgg aaaacggcat catgacagtt cttcgctgat cattttggcg
     5281 tacatcttgg aaaccggttc atgtttatat ataataggtt taataaattg cgagggtaat
     5341 ctttgaacgg aggtaacgca gcaaaacgga aaggggcagc agcaaatgga aagagacgat
     5401 atcgaaatgg caaatgcagc cgaaaaagct ggaaaatata tcaggaaagc catggatatg
     5461 ggcgccgacc acgcagtatt tttcaggacc gaggacatcg cattcgatcc gaggtcgctg
     5521 cttaaatgca tgttcggctg cagttcatgg ggcaaagacc atacctgccc gtcaaggccg
     5581 ggttcgctga agccatggga atatgagcag atattcagaa gatacagttg gggcatcata
     5641 atacactccc acagcaaaaa aatctcacag gacatatcat tcgcaataga aagccaggct
     5701 ttcacggacg gctactacat ggctttttcg ctcagcgatt gctccatctg ccccgagtgc
     5761 ggcggaaaaa ggggaatgcc atgtgccgat ccgaaaaaag caaggcctgc attccacagc
     5821 gtcggcatcg atgtgttcaa aacggtcagg aaattcggcc tgcccataga aacgctgaag
     5881 acggaatcgg acgagcagaa ctggtactct gcagtgttta tcgaataaac cggcagccag
     5941 gttttcaatg atcctgactt cggtgacccg gcagtctgat cgtttgatcc ggacattcgg
     6001 tcatattaag tgacgcgtta agcgttgacc ggcccccgac cattcgatcc tgataatcaa
     6061 cagcttggca ggtaattagc tgaataaata aatataggag tggttgcaat ggtatctgaa
     6121 aaaatacttg cgaatctaaa aagatcatcg tggataaggg caatgttcga acagggagaa
     6181 aacctccgca aaaagttcgg agccgacaac gtctacgact tttcgctcgg caaccctgac
     6241 ctggaaccgc cggagatagt cagggacatc ctcaaaaagg cggtgctttc ggacactccc
     6301 aaaatgcacg gttacatgag caatgcaggt taccctgacg taagggctgc tgtcgccgaa
     6361 aacctgagga agtccaaggg cgtccctgtc gaagcctgcc atgtggtgat ggagtgcggt
     6421 gcaggcgggg ctatgaacgt ggtgcttaaa accatcctca acccgggaaa cgaagtaatc
     6481 gtacttgctc cctatttcgc cgaatacctg ttttacatag acaaccacgg cggcgttccg
     6541 gtcatcgtgg acacggaccg tgaaacgttc cagcctgacg cgcaaaagat ctacaatgtc
     6601 ataaccccga agaccaaggc cataattatc aactctccga ataaccctac cggcgtcata
     6661 tacagccgga gttctctcga gagcatttcg gaagttattg aaaaaaggca ggaggaattc
     6721 ggcaacacca tatgcgtcat atccgacgag ccttatgaca aaatagtata tgacggcgcc
     6781 gcagtcccgt cagtgttcga agtctttaaa aactccgtca tcgttaactc gtacagcaaa
     6841 tccctttcac tgccgggtga aaggataggc ttcgccgcgg tcaatcccag gattggcgat
     6901 accgcgccgc tggtggacgg gcttattttc tccacgagga cactgggctt cgtcaacgcg
     6961 cctgctctct tccagcgtat acttcccgga tcgctcgacg ccaaggtggc ggtcgatgcg
     7021 tataagaaga gacgcgacat gctctacgaa atcgtcacga aagccgggtt caaatgcctg
     7081 aaaccgcagg gcgcatttta cctgttcccc cggtctccga tagaggacga cgtgaaattc
     7141 tgcaacgagg cgctcaaata caacctgatc atcgtgcccg gcacagggtt cggattcaaa
     7201 ggcttcttca ggctggcata ctgcgtggac gaaaagacga tagccaactc ccaaaaggca
     7261 tttgaggcgc ttgcagcgga gtataaataa agcggcgcct tgcgcgccag acgtggcgcc
     7321 ctgtatatca aatgtggtgc ctgacacgtc aaatacggcg cctggcgcgc cagtcagccg
     7381 gtaattgttc atcactgtat tgattattgc tgactaactg gcgcgccagg tgccgttttg
     7441 taccgttttt tcatttccgg gcggtgatct gaccgatcat ccaaattcct cacgtttttt
     7501 gtaccaattt ttcgtttctg ggcacataag tatggatttc tttcaataat gagtaacata
     7561 atatggcata ttcggcatta tgtgaatggt ttaccctgga gaaaagcttt cctgaaggtt
     7621 tttctgtccc agaaacggaa attttgcaca ctgaaaatca aaattcaatc ttttgctgta
     7681 atcaccagca gcagcccttc ctttacctcg acgtacgcct cgtcttcgga gaacctgttg
     7741 ctgacgccaa gggacgaacc agtcttcagc actgcgttgt caagcgggaa atacaggccg
     7801 cgggtggaaa ctcccgaggc gtccccgccg aaaggcagta atgtaacaaa aacgccttcc
     7861 tcccgcttca gcctgatgct gccgctgaca aggcgcactt catacttatc gtcggcaatg
     7921 atgccttcca cattcgcatc atgcatcttc tttaataaaa aaaggttgga tatcgaatga
     7981 tccagccttg aaccggctgc cccgagcagt atgatcctgc tgcagccccg gcttatcgct
     8041 gtctcaacgg caagttcgct gtccgtcatg tccttttcga cgggaaaccg gagtatctcg
     8101 gttcctgcct cttcgagccg gctgtaatcg cttctgtcta tagaatcgaa gtctccaagg
     8161 gcgatgtcgg gcacgatgcc aagagccctg cagtggcgtg ccccgctgtc agcggatatg
     8221 accagttccg cattcctgca gtacttctcc gcgtggctgt aatccccgat ctttccgccg
     8281 cagaatacca cgcaagtcat atggtaccac catacgcctt ttgcctcagg ctgctcatta
     8341 tttcagcggc gtctggcgct ccgaatatgg tcgtgcctgc aacgatcaca tttgcacctg
     8401 cctgtgtcac atcccatatg ttagaaaggt cgatacctcc gtcgacctcg atatcaaggc
     8461 tcaggccgcg ttttacggca atttgcctga gttcgcgtat cttttccgtc atgttttcta
     8521 tgtaatgctg gccgccaaat cccggatcca ccgtcatcag cagcaccata tccacatctc
     8581 caagcacggc atcaagcgta ttcagaggag tggcgggatt cagcaccact gagggccttg
     8641 ctccgcattc ccttatcagt tctatggtac ggtgcaggtg cggattgttt tctacatgca
     8701 ctgatattat attagcgcct gcatctgcaa aatcttttat gtaacggtcc ggatcctcaa
     8761 tcatgagatg tacgtcaaac gggagttccg tgactttcct gaggcttttt atcacaggcg
     8821 ggccaatggt tatgttcggt acgaaatgtc cgtccatgac atccaggtgt atcatatctg
     8881 cgccagccct ttcgaccctg gatatttctt ccccgagctt cgaaaaatcc gatgccagga
     8941 tcgacggtgc gattttaatc attccccttc atccctttcc tgccgccctt tttgtatttt
     9001 gcttcgtctt cctgtttcag tgttttataa agttcagcaa agcgggcgta tcttcctttt
     9061 tctatcgacc tttcatcaac agccttcttc acggcgcagt ccgcctcaaa gacgtggctg
     9121 cagcctttga acctgcactt cggtatatgt tcagcaaatt cgggatacag gaactgtagc
     9181 ctgtcatatt ctatacccgt aagctcaaat gaactgaaac cgggagtatc gacgaggtat
     9241 cctccgcccg gaagttcaat cagttctgcg tgccgggtgg tatgcctgcc tctttctatt
     9301 ttttcactta cactcccggt cttcatcagc acggtgtcca tgaccctgtt gagcaacgtc
     9361 gacttcccga ctcccgactg gcccgcgaac accgatatat gccccttcaa tgcttccttc
     9421 agcttgctga agccatccga ctcccggaaa ctggtctcga tgacctcata acctgctttt
     9481 atgtatggct cccgcagcct ggcccgtttg ccgccttcat ccagatcgat cttgttgatg
     9541 catactaccg ccctgatacc cttgtcctcc gcagttacaa gtatcttgtc cagcagcatc
     9601 aggtcgggat ccggcgattt cgccgcgata actgcgatga tctggtctac attcgcgact
     9661 gcaggcctta caaggacact gctcctttct gttatttctt ctatattccc cttttttcct
     9721 gcaggatctg tcacagaaaa aacgaccctg tcgccggtca gcggggtcag ccctttcttc
     9781 cggaatattc ctctggccct gcactcataa acaccgtcat cttttgccgc aacataatag
     9841 aacccggcta tccctctgat aataatgcca gacggcaaat atccaacctc ctccgcatct
     9901 gcaaccatga atttgataaa taacgcgtct gttgttactg attctggata tcagtttcca
     9961 aagccagatc attatccaga taaacgatca catgcgtcgt ccctgtctca ccaagggtga
    10021 tcggaatact gaacgggaac aggtccctcc ttacatactg gttcatctcg atcttccgca
    10081 cattcgtgtc ggacggagtg gattccacat aaacaagcac ttcttcgccg tatgtgcggc
    10141 ttgggttcaa agtgatcgaa atagctttcg tcttcgcggt acttacctgc tgggatcctc
    10201 cgtcatccgg accggacgga gcatcaccgc cccttccttc ttcaggatgc tcatctattc
    10261 cgaatgtcag gtccactgcg gtatattcat ccaccacttc attcggaaga gggctctgct
    10321 ttatcacttt agcgacatcg cttactatat ctttaggtaa aaaatcacct atcgtgaggt
    10381 tgttcttcag tattgcgttc tccgcctctg tcctggtcat tccgccaaaa tcgggcactg
    10441 tcaccttttc aagcttcggt cctgcgctca catatacttt gacaacggta tcaggcttta
    10501 ccatttcgaa tgcttcaggc tcagtcttta taacaaggtc tttgcccacc ttatcattgt
    10561 tctccctgac tatttcaggt accagccctg cattggttat ctccacttcc gcgatcctgt
    10621 actcctgtcc cctgatatca ggtatttcaa ccatatgggg cccgtcgctt acggtgaagt
    10681 ttatcgtgct tccgggttta agagtattgc cgaccgctat gtcctgcgat atgatgactc
    10741 cttttttgac agtttcgtta aacaccctct cattgtccac agcggtaatg tcatattggc
    10801 tgagcagctc cttcacctcg tcgaagtcca tgttcgtata gtcttcaacg acgaacgaag
    10861 ccgtatcgtc aggcgctatc atcggaataa cgagtttgat gccaaggtag acgacaagtg
    10921 ccatgatcac cacgccggac gctatgccca ggcagatgct cagccagtcc cttttcttcc
    10981 tgttgtctgc ttcaccatct tcttccatgt atccttcatc tccgtcatcg gtattttcct
    11041 ttattatatc aaccccggta ccgactgcct gcattttttt cgtaggcagt tcttcaacgg
    11101 cgttgcttcc gtttattttc gtatcgggat ctgccaggac cctctgcagg tcggccagca
    11161 tctcggatgc ggtctgatac ctgaggttct ggtccttctt cattgccttg attatgaggt
    11221 cgtttactcc cttcgggatc ttcgggttga tgtcgatggg cttttcaggc ttttcctgga
    11281 tatgcttaag cgcaatcgca acaggcgact ccccgtcgaa cgggactttc cccgtgacca
    11341 tttcataaat ggtgatgccc agtgagtaaa ggtcggattt ttcatcgatg aaaccgcctc
    11401 ttgcctgctc cggggagaaa taatggacag agccgatggt gcttccaacc attgtaatgg
    11461 tcgcagacgt gacagccctg gcaatgccga agtcggtgac tttggctatg ccatccttcg
    11521 ttatcatgat gttgtgcggt tttatatcac ggtgaacgat gaaattctta tgggcatgtt
    11581 cgagagcgga acatatctgg atcgcgatcc cgacagcttc cttccatgga agtgcgccct
    11641 tctggctgat gtattccttc agcgtgatgc cgtctataag ctccatcacg atgtactgga
    11701 tgtcgtcctg tttgcctacg tcatatatag atacgatgtt cggatggttc aggctcgccg
    11761 cggcctgcgc ctccacccgg aagcgcttta cgaattcctc gtcgtctgta aactcactgc
    11821 gtaatatttt aacggcaaca aagcggttca gaagcaggca cttcgcctta tagacgactg
    11881 ccatgccgcc tccgccgatc ctttccagca atttatatct atttcccaat atctgacctt
    11941 ccatatccac accttcacta acactttata acaatgaccg tgatattgtc ctcaccgccc
    12001 tgtttgttgg cggcttcaat aagctttgca cacgcgtgtt ccgggtcgct gccggaaact
    12061 atatcggcta tatcgctttc actcaccata tttgtcagtc cgtcggtgca aagtatgtat
    12121 atatcatcag cctcgatctg atggcttatt atgtccactt ccagttcggg agaagagccg
    12181 atcgcccgcg taatgatatt cttccgcggg tgtttctctg cttcctccct ggtcagtttg
    12241 ccctgcctga ccagttctcc gatatacgaa tggtcttcag tcagctgtcg gatggctccg
    12301 ttccttatca gatagagcct gctgtctccc acatgggcgg ccgtgagcgt ggatccggca
    12361 ataacggcca tggtcagcgt cgtacccatg ccgctgacct cagcgcttcc aagcgatctc
    12421 tcataaacgg cagtgttgac tttctttacc agcgcttcga gcttaccgct caggtccgcg
    12481 ctgtcaaagc agccttcagc acctatattg tcccttatgt actcaacggc catcctgctt
    12541 gcgacttccc cgctgttgtg cccgccgagc ccgtcggcga tgacgaagac gcaggacgtg
    12601 tccgagcgac ccg
//
LOCUS       DAOSCC010000043        12583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3372_length_12583_cov_4.419221, whole genome shotgun sequence.
ACCESSION   DAOSCC010000043 DAOSCC010000000
VERSION     DAOSCC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_12583_cov_4.419221"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLG72_03770"
     CDS             complement(<1..473)
                     /locus_tag="PLG72_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HOJ79948.1"
                     /translation="MQVKVENAGKNVVQLEIEVEAEKFEQGMQKAFARNAKKFNIPGF
                     RRGKAPRHLVERYYGEQVLYEDAINEVCPDAYEQAVEENDIHPVDRPEIDIKQIGKGK
                     SLIFTATVTVRPDVELGEYKGVEVEKSTAVVTDEDVDKEIERVRERNSRLIAVED"
     gene            complement(608..1348)
                     /locus_tag="PLG72_03775"
     CDS             complement(608..1348)
                     /locus_tag="PLG72_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ79949.1"
                     /translation="MALFAISDLHLALSVDKPMDVFGPRWANYMDKLKENWCSVVGED
                     DYVIVPGDISWATYLNEAHEDFRFIHELPGKKIISKGNHDYWWTTMSKMEKFVSENGF
                     TSISFMHNNSFSIEGMTICGTRGWDMPGEVGFGADDARIYQRELQRLEMSLQKGMSDI
                     SSDPAFADNTIIAALHYPPFNSRGEFSEFMDIMKKYGVKICIYGHLHGDACKAAVEGL
                     VDGIMFRLVAADHLGFMPEKLCEDVSGQ"
     gene            complement(1610..3478)
                     /gene="uvrC"
                     /locus_tag="PLG72_03780"
     CDS             complement(1610..3478)
                     /gene="uvrC"
                     /locus_tag="PLG72_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ79950.1"
                     /translation="MFDIQQELAKLPDKPGVYIMKDKNGSVIYVGKAKVLKNRVRQYF
                     QDSAAHSPKVEAMVAKIAEFEYIVTDTEFEALILECNLIKDKKPRYNILLKDDKTYPY
                     IKVTVNEEYPRIYMTRRVEGDGARYFGPYPNVHSIRETINLIRKIFPIRTCKRVLPRD
                     IGRNRPCLYYHIKQCLGPCAGHVDKKEYQAVVRDVCSFLDGKQDAIIEKLEKEMQEAA
                     ERLDFEQAAALRDKLISVRNIVQEQKVFTVSGSDQDVVGFAASKTDTCIQVFFVRGGK
                     LLGRRFFIIQGSGEAELSETASSFLKQFYGTADLIPPEIITGADVEDRELIEDWLSAR
                     RGGRVHVRVPKRGEKLRMAEMVAKNAKIELDRFNDEMRKADGVQEGLKELAGILGLDG
                     IPERIEAYDISNTGSSEIVASMVVFENGTPAKKEYRRFRIRTTDTQNDYASMQEVLHR
                     RLTHAIKEKIDTGEQDPDKKDHGKFSRLPDLIMVDGGIGHVNAARQVMQDLDVDIPVC
                     GMVKDDKHRTRGLVAPGNEADLTGNIVALRLVTSIQDEAHRFALDYNRMLRTKRYSGS
                     ILDEVEGIGPKRKKSLLKHFGSVARMRQAGVDDFQAVEGISRPMAERLYDFFHSAR"
     gene            complement(3482..3751)
                     /locus_tag="PLG72_03785"
     CDS             complement(3482..3751)
                     /locus_tag="PLG72_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ79951.1"
                     /translation="MISKEILIKSPQGLQSKAAAIFIQKATGFESNIWITKGERKANG
                     KSLLGVLSLGIKGDSVILLTADGSDEAEAIAELENYLVSDTGENV"
     gene            complement(4086..5003)
                     /locus_tag="PLG72_03790"
     CDS             complement(4086..5003)
                     /locus_tag="PLG72_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79952.1"
                     /translation="MKKLAALLAIFILLAAWAVPVLGEGIYVNSENGDKIKIFEDAEV
                     TAPVRGNVIVVLGDVRVESRVDGHVVTVFGDAAINAEVTGQVVSMFGKATLEDGARIN
                     GDVISVGSVEKSPGAVIGGQEVRILGESMNLDIDALFYLRLVILVLFMLAVLLTGLLS
                     LLISRTRYRMISNNLEKNPVRKLVLGILSFIGATSLLAILLLTLIAPLLYIILLIIST
                     VPACIYLGRMILKAFSRKNGIFIEFITGLITATLVKLVVLLIIPQQNLLLAAIVTGLI
                     NFLIYSFGMGILMEQHYLQGNKTNAQKET"
     gene            complement(5000..5578)
                     /locus_tag="PLG72_03795"
     CDS             complement(5000..5578)
                     /locus_tag="PLG72_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ79953.1"
                     /translation="MNCDTSKELMMKYFDGEMDEAEEKQFREHLKACSGCSEEFGCME
                     AIFSTLDEKVEIEPPDDFEARVMDKVALIENQKKERSEKRIVLLYNFATMLSIVLLLI
                     FVADMKEVSVFSAFERLGEYFGSFSTVTAAIFGAVKDLTGLLIDALAAVADIALSIVR
                     SYYYAFIVLILAVLAIQRLIHYVGTHTGEEAE"
     gene            complement(5575..6129)
                     /locus_tag="PLG72_03800"
     CDS             complement(5575..6129)
                     /locus_tag="PLG72_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ79954.1"
                     /translation="MELTDYELIQKCLAGQHEYFEELVTRYKKLIFSVVYNMINDKEE
                     VSDLSQEVFIRIYKALDRYDPKYKFSTWAVRIATNLCLDIHRKKKVNSTSIDEIGDLS
                     DGVDTPEDSYLRKERSERIRRAIQELPEKYRTPIILFHQNGLSYEEMTKVLGEPMTII
                     KNRLYRARIMLREALMKERKEEAL"
     assembly_gap    6156..6255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6853..7197
                     /locus_tag="PLG72_03805"
     CDS             6853..7197
                     /locus_tag="PLG72_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOJ79955.1"
                     /translation="MTVTNVTRKTVLADKCEVASTFFARFRGLQFRKELPPGHGLLIT
                     PCNSIHMFFMRFPIDALFIDSENRIVHIYEGIKPWRVSGIIKNAHCVLELPSGTVYST
                     GTVTGDLLDMQP"
     gene            complement(7330..7785)
                     /gene="rimI"
                     /locus_tag="PLG72_03810"
     CDS             complement(7330..7785)
                     /gene="rimI"
                     /locus_tag="PLG72_03810"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOJ79956.1"
                     /translation="MLNNLEIVRAGKEHIDDIMTVENLSFKIPWSRQSITDEFLHNEK
                     AVYFCALINGRAVGYAGMWQVCDEGHITNIAVHPEFRRSGIGSALMEALIDEAENRGL
                     AALTLEVRKSNHGARSMYEKYGFEDGGMRKAYYADNNEDAIIMWKRLKD"
     gene            complement(7800..8525)
                     /gene="tsaB"
                     /locus_tag="PLG72_03815"
     CDS             complement(7800..8525)
                     /gene="tsaB"
                     /locus_tag="PLG72_03815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ79957.1"
                     /translation="MRALAVDTSTATAGIAVADENGLLAEFLLKDKKTHSQKLVPMMK
                     EVLESLKLDLSDIDVFAAVTGPGSFTGLRIGVTTIKTMAYALKKPVVGIPSLDALANA
                     AVVPDDALVCPIMDARNSQVYTALYKPRNGLMANLSGYMGLHISELVKQIGEKYAGAN
                     IMFAGDGVLLHRDFLKIQLKERCSFMPPFTYQMMAAPAAQLALALHARGESTDCAGLM
                     PFYLRPSQAEREYERKHAQGSRE"
     gene            complement(8506..8985)
                     /gene="tsaE"
                     /locus_tag="PLG72_03820"
     CDS             complement(8506..8985)
                     /gene="tsaE"
                     /locus_tag="PLG72_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ79958.1"
                     /translation="MKEFRASSVADTVELGRKLGSMLQRGDNVCLFGDLGTGKTAFTS
                     GIAKGLGISGYITSPTFTIVNEYEGRLPLYHFDVYRIGDVDEMLDTGYYEYIDGDGVT
                     VIEWADLIREILPSERIEVYIEKNDPEGSDSRTITVSFIGDRTAEYERRLTDESACG"
     gene            complement(8982..10160)
                     /locus_tag="PLG72_03825"
     CDS             complement(8982..10160)
                     /locus_tag="PLG72_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ79959.1"
                     /translation="MLLVKNAKILTMAGIDYNDGYIAAEGEKITALGNDAAELARLEA
                     AASEVIDAKGRYVLPGFVDAHCHVGMWEDSVGFEGDDGNETSDPVMPHLRAIDAVYHA
                     DRAFVEARENGVTTVVTGPGSANVIGGQFAALKTYGRRVEDMVIKAPVAMKVAFGENP
                     KSVYHEKRQAPMTRMATAALLRESLMKAVEYKKMKEDYERDSENFDKPDYDIKMEALL
                     KVLNGEIPLKAHAHRADDILTAIRIAKEFGVGITIEHCTEGHLITDILLEENVSVITG
                     PFLTDRSKIELRNQSARTPGILSRAGIKTAIMTDHPVTPVQYLPLCAAVAVKEGMDET
                     EALKAITINAAEITGIADRVGSLEAGKDADIIIMDGHPLELKSRVLYTIINGQVVKRI
                     "
     gene            complement(11227..11622)
                     /gene="rpsI"
                     /locus_tag="PLG72_03830"
     CDS             complement(11227..11622)
                     /gene="rpsI"
                     /locus_tag="PLG72_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ79960.1"
                     /translation="MAAKIQYYGTGRRKKSIARVRLVPGEGKITVNDRNIDDYFGLET
                     LKVIVKQPLVLTETLGKFDVLCNVVGGGLSGQAGAIRHGIARALVKADEEFRPALKKA
                     GFLTRDPRMKERKKYGLKKARRAPQFSKR"
     gene            complement(11644..12078)
                     /gene="rplM"
                     /locus_tag="PLG72_03835"
     CDS             complement(11644..12078)
                     /gene="rplM"
                     /locus_tag="PLG72_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ79961.1"
                     /translation="MKTYMAKAEEVKKKWYVVDAAGKPLGRLASVVASILRGKHKPIY
                     TPHVDTGDYVIVINAEKAVLTGKKLDQKVYRRHSGWPGGLKETKYRQLMENSPEKAVE
                     LAVKRMLPHNSLGRAMFRKLRVYRGPEHEHQAQKPEVLEIDV"
BASE COUNT         2864 a         3466 c         2525 g         3628 t
ORIGIN      
        1 ctgtcctcaa cggctatgag ccttgaattc ctctcacgta ctctttctat ctccttatcc
       61 acatcctcgt cggtgacgac ggctgtcgat ttctctactt caacgccttt gtactcacca
      121 agttccacgt caggtctgac agtgaccgtc gcagtgaata tcagggactt gcccttgcct
      181 atctgcttta tgtctatttc aggcctgtcc acggggtgga tatcattctc ttctaccgcc
      241 tgctcatacg catcgggaca cacttcgttt attgcatcct cgtacagcac ctgttccccg
      301 taataccttt ccaccaggtg tcttggcgct ttgcctctcc tgaacccggg tatgttgaac
      361 ttctttgcat tcctggcaaa tgctttctgc atcccctgct caaatttttc cgcttcaacc
      421 tctatttcga gttgaacgac attcttacct gcattttcca ctttaacctg catgtgtctt
      481 cattctccta tcattacaga ttgcttgatt cgacaaaact tacgggggta agcatacccc
      541 ctcgattaac gcatacatta taacaaaata ttatcataaa gccaatacaa aacaattttt
      601 tgttgaatta ttgcccggaa acgtcctcac acagtttctc gggcatgaag cccaggtggt
      661 cggcggccac cagcctgaac atgatcccgt cgaccaggcc ttcgactgct gctttgcagg
      721 catctccgtg cagatgcccg tatatgcata tctttacgcc atacttcttc attatatcca
      781 tgaattccga aaactctccc ctggagttga acggtggata atgcagcgcc gctattatgg
      841 tattgtctgc gaaggccgga tctgacgaga tgtccgacat tcccttctgc agtgacattt
      901 cgagcctctg cagttccctt tggtaaattc tcgcatcatc ggcgccgaac ccgacctcac
      961 ccggcatatc ccagcctctc gttccgcaga tcgtcattcc ttcgatgctg aaactgttgt
     1021 tgtgcatgaa gcttatcgag gtaaagccgt tttcgctgac gaacttctcc atcttgctca
     1081 tggtcgtcca ccagtaatca tgattcccct tggatatgat ctttttgccc ggcagttcat
     1141 ggatgaacct gaaatcctca tgcgcttcgt taagataagt cgcccatgaa atatcgccag
     1201 gcacgatgac gtaatcgtcc tccccgacaa cggagcacca gttctccttc agcttgtcca
     1261 tatagttggc ccatctcggc ccgaacacat ccatcggttt gtctacactc agcgccagat
     1321 ggagatccga tatagcaaac agcgccataa tccattcctt cctgtttcag gggacagttc
     1381 ttcatttttg gggacagttc tcaactttta gcgttgggga cagtcctgct tttcggggac
     1441 gtttctcaac tttcaaagtt tacgccggag tcccggcttc cggtaaggct cctgctccct
     1501 tgaaaagtaa cctcattgca ggatataacc cgcttttttt aagaataacc ccctgttata
     1561 gggataatta acctcatgct atccttgtat actcttatag ccatgtctgt cacctggccg
     1621 aatggaaaaa atcgtacagt ctctccgcca tcggcctgct tatgccctcc actgcctgaa
     1681 aatcgtcaac ccctgcctgt ctcatacgcg cgacggagcc gaaatgctta agcagcgact
     1741 ttttcctctt cggcccgatc ccctccacct catcgagtat ggatccggag tatcttttgg
     1801 tcctcagcat cctgttataa tccaacgcaa acctgtgtgc ctcgtcctgg atcgacgtga
     1861 caagcctcag cgcaacaatg ttgccggtca ggtccgcctc attccccggg gccaccagtc
     1921 cgcgggtcct gtgcttatcg tccttgacca ttccgcatac cgggatgtcg acatcgagat
     1981 cctgcatcac ctgccttgcg gcatttacat gtccgatgcc gccgtcgacc atgatcaggt
     2041 caggcagcct gctgaacttg ccgtgatcct ttttatcagg atcttgctct cctgtatcga
     2101 ttttctcctt aatggcatgc gtcaatctcc tgtgcagcac ttcctgcatg cttgcatagt
     2161 cgttctgcgt atcggtggtc cgtatcctga aacgcctgta ttcctttttc gccggcgtcc
     2221 cgttctcgaa taccaccatc gatgctacta tctcggaaga accggtattc gatatatcat
     2281 aagcctcgat cctttcagga attccatcga gtcccagtat acccgcaagt tccttcagcc
     2341 cttcctgcac tccgtcggct ttcctcatct cgtcgttgaa gcggtcaagt tcgatctttg
     2401 catttttggc gaccatttca gccatacgga gcttctcgcc tctcttaggc acccttacat
     2461 gtaccctgcc gcccctcctg gcgctcagcc agtcttcaat cagctccctg tcctcgacat
     2521 cagcgcctgt gatgatttcc ggtggtatca gatccgcagt cccgtaaaac tgtttgagga
     2581 acgatgaagc cgtttccgac agttcggctt ctccgctgcc ctgtatgatg aaaaaccttc
     2641 gccccagcag ctttccgccc cttacgaaaa acacctggat gcaggtatcc gtcttgcttg
     2701 cggcaaaccc gaccacatcc tggtcggagc ccgatactgt aaagactttc tgttcctgga
     2761 ctatgttcct cacgctgatc agtttgtccc tcaatgcggc ggcctgctca aaatccagcc
     2821 tctccgccgc ttcctgcatt tccttctcca gtttttcgat tatcgcatcc tgtttcccgt
     2881 ccaggaacga acagacatcc cgtaccaccg cctggtattc ctttttgtcc acatggcccg
     2941 cgcatggccc caggcactgc tttatatggt aatacaggca cggcctgttc ctgcctatat
     3001 ccctgggcag gacacgcttg cacgtccgta tcgggaatat tttcctgatc aggtttatag
     3061 tctcgcgtat cgaatgcaca ttgggataag gaccgaaata tcgggccccg tctccttcga
     3121 cccgtctcgt catgtatatc ctgggatatt cttcgttgac tgttactttg atataggggt
     3181 acgttttatc gtctttgagc aggatgttat aacgcggctt tttgtccttg atcagattgc
     3241 actccagtat gagcgcctcg aattcggtgt ctgtgacgat gtattcaaac tccgcgatct
     3301 ttgccaccat cgcctccact ttgggagaat gggcggcaga atcctgaaaa tattgcctga
     3361 ctctgttctt cagcactttt gccttgccga catatattac agagccgttt ttgtccttca
     3421 taatataaac gcccggttta tcgggcagtt ttgcaagttc ttgctgaata tcgaacatgg
     3481 tctagacgtt ctcacctgta tccgatacca agtaattttc caactcagca atggcttccg
     3541 cctcatcgct tccgtcagca gtaagcaata tgaccgaatc gccttttata ccaagtgaca
     3601 aaactcccag caggctcttg ccgttagcct tcctttcgcc cttcgtgatc catatgttcg
     3661 actcaaatcc ggtcgccttt tgtataaaaa tcgctgcggc cttcgactgt aacccctgcg
     3721 ggctctttat cagtatctct tttgatatca ttatttacct ccatttactt ccgtataact
     3781 atacgtttat ccaatgccga tatatattgt tcctcttccg gtacaaagca acgcccgtgc
     3841 ggacgttcca tgcatgtatc cttattcaat cccattatac caaatgtatt ttctcaatac
     3901 catattatct ttaagaaacg tccctaaacg taagtagaaa taaaaacggc cggactcagt
     3961 tccggaccgt cgttatttac acacgaatgt tttcgctgca gccgctgcgg gactttgccg
     4021 gtactgcagg acttcgccga ttctgccggg gacctgcctc tgctgctgtg agctgcccgc
     4081 tgctttcagg tttccttctg cgcattcgtt ttattaccct gcagataatg ctgttccatc
     4141 aggatgccca ttccaaagga atatatcagg aagttgatca aacctgtcac tatggctgcc
     4201 agcaaaaggt tctgctgggg gatgataagc agaacgacaa gcttgacaag cgtggcagtt
     4261 atcagccccg tgatgaattc aatgaaaata ccgtttttcc ggctgaatgc cttgaggatc
     4321 atcctgccca aatatataca tgccggcact gtcgatatga tcagcaatat gatgtacagc
     4381 agcggcgcta taagcgtcag cagcagtatc gccagcaacg aagtcgcccc gatgaatgac
     4441 agtatgccga gcaccagttt tcttaccggg tttttctcca ggttattcga gatcatgcgg
     4501 tacctggtcc ttgagatcag gagcgacagc aacccggtca ggagaactgc cagcatgaac
     4561 agtaccaaaa tgacaagcct cagatagaac aacgcatcta tatcgagatt catggactcg
     4621 ccaagtatcc ttacttcctg cccgccaata acagcgcccg gggatttctc gacagagccg
     4681 acggagatca catcgccatt tatcctggct ccatcctcca gagtcgcttt tccaaacatc
     4741 gataccacct ggccggtgac ttcggcattt attgccgcat caccgaaaac agtgacgaca
     4801 tggccgtcga ccctgctttc gacccggaca tcgccaagga ccactataac gttcccccgg
     4861 acaggcgcgg tcacttccgc gtcctcaaaa atctttatct tatcgccgtt ttcagagttt
     4921 acatatatgc cttctcccag gaccggaacg gcccatgccg ccaaaagtat gaaaatggca
     4981 aggagcgccg caagtttttt cattcagctt cctccccggt atgtgttcca acataatgga
     5041 taagcctctg gatcgccagt actgccagta tcagcactat gaatgcatag tagtatgatc
     5101 tgacgatcga gagtgcgata tctgccaccg cagcaagcgc atctatcagt aatcccgtaa
     5161 gatccttcac tgctccgaat attgcggccg taacagtcga gaaggatccg aaatactcgc
     5221 caagtctttc aaaagcgctg aatacgctga cttctttcat atccgcaaca aaaatgagca
     5281 acagtacgat cgaaagcata gttgcgaaat tgtaaagcaa tactattctc ttctcgcttc
     5341 tttctttctt ctggttctcg atcaatgcaa ctttatccat gacccttgct tcaaaatcat
     5401 ccggaggctc gatttccacc ttttcatcaa gtgtagagaa gatggcttcc atgcagccga
     5461 attcctcgct gcagccgctg caagccttca aatgctctct gaactgtttt tcctcggctt
     5521 catccatttc tccgtcaaaa tacttcatca tgagttcctt cgacgtatca cagttcataa
     5581 agcctcctcc tttctttcct tcatcaatgc ctctctcagc attatccttg cccgatacag
     5641 cctgtttttt attatcgtca tcggctctcc gagaaccttt gtcatttcct catacgataa
     5701 cccgttctgg tggaacagta ttatcggcgt cctgtatttc tcaggcagtt cctgtatcgc
     5761 cctccggatc ctttcagacc gttccttccg cagatagctg tcttcaggcg tgtccacccc
     5821 gtccgataga tccccgatct catctatcga tgtcgaattt accttcttct ttctgtgtat
     5881 gtccaggcag agattggtgg caatgcgaac cgcccaggtg gagaacttat acttgggatc
     5941 ataccggtca agagccttgt agatcctgat aaagacttcc tgggaaaggt cgctgacctc
     6001 ttccttgtca ttgatcatat tataaacgac gctgaatatc aactttttat atcgtgtcac
     6061 aagttcttcg aaatactcgt gctgccctgc cagacacttc tgtatcagtt catagtcggt
     6121 cagctccaat cggttctcac caccttgcct atattnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnngggac agttctctac atagtagaaa ttcgttgcgg gttgagaact
     6301 gtcccctttt gattggttta gctcgacact cctcagcaaa gaagcagatt ttcacgggtt
     6361 gaggagtgtc ccctttcgtt tggtttgact gggacagtcc tctacatagt agaaattcgt
     6421 tgcgggttga gaactgtccc cttttgattg gtttagctcg acactcctca gcaaagaagc
     6481 agattttcac gggtcgagga gtgtcccctt tcgtttggct tagtttgggc actccgggtt
     6541 aggggacagt tctcaaaaag atgcaaaaca ccgcaattgt gagtgcttct ttgcaattga
     6601 cccaaacacg acactttccg ataattatat tggggagcaa tattagggga cagttctcaa
     6661 gaaagttaaa caggatgcat gactaaatga gtaaagcaat aaggatcgct catcgggaat
     6721 acgatatgtg taaaaagctg agaagtgtcc ctgttgatcg gacagtcgag aaacgtctcc
     6781 tatggccata aagttgagaa gtgtccccat gaggtgatat aaagttgaga agtgtcccca
     6841 tgaggtgata ccatgacagt aacgaatgtg acccgcaaga ctgtgctcgc ggacaaatgc
     6901 gaagtcgcgt cgaccttttt tgcaaggttc agagggctcc agttcaggaa agagctgcct
     6961 cctgggcacg gcctgcttat aactccgtgc aattccatcc atatgttttt tatgagattc
     7021 ccgatcgacg ccctgttcat agattcagaa aacaggatag tccatattta tgaagggata
     7081 aagccgtgga gagtatccgg gataataaaa aacgcccact gcgtgctgga actgccttca
     7141 ggcaccgttt actcgacggg cacggtcact ggcgatcttc tggatatgca gccatgacta
     7201 tctgtgctta tctgctgacc atcccgttta tatcaattca gctatatacg aactgccacg
     7261 atatccttgc catgcaatta cggcatcaca tggcacccaa tatttttccc gttaccgttt
     7321 tgttatatcc taatctttca gccgcttcca cataattatc gcatcttcat tgttgtctgc
     7381 ataatacgct tttctcatgc cgccgtcttc aaacccgtat ttttcataca tggacctggc
     7441 gccatggttg ctcttcctta cttccagcgt aagcgccgcc agaccgcgat tttccgcttc
     7501 atcaatcaac gcctccataa gggcgctgcc aatgccgctg cgcctgaact cgggatggac
     7561 ggcaatgttg gtgatgtggc cttcgtcaca tacctgccac atccccgcat accccacggc
     7621 ccggccgttt atcaaagcgc agaaataaac cgctttttca ttatgcagga attcatccgt
     7681 tatcgactgt cttgaccatg gtattttaaa actcaggttt tccaccgtca taatgtcatc
     7741 tatatgttct ttccccgccc tgacgatctc caggttattt agcattacaa catcctccgt
     7801 cattccctgc tgccctgcgc atgctttctt tcatactccc tctcggcctg ggaaggcctg
     7861 aggtaaaacg gcatcaagcc ggcgcagtcg gtgctctctc cccttgcgtg cagcgcaagc
     7921 gccagctgcg cggcaggagc agccatcatc tggtatgtga aaggaggcat gaaactgcag
     7981 cgttctttca gttgtatttt aaggaaatcc ctgtgcagaa gcactccgtc gcccgcaaac
     8041 atgatattcg cccctgcata tttctccccg atctgtttta ccagttcgga aatatggagc
     8101 cccatatacc ctgacaaatt ggccatcagt ccgttccggg gtttataaag cgcggtgtag
     8161 acctggctgt tcctggcgtc cattataggg cacaccagtg cgtcatccgg taccacagcc
     8221 gcattagcca aagcatccag cgacggtatc ccgacgaccg gctttttcaa tgcgtacgcc
     8281 atggtcttta tcgtcgtcac gccgatcctg agcccggtaa acgacccggg accagtcact
     8341 gccgcaaaaa catctatatc ggacaaatcg agcttaaggc tttccagcac ttccttcatc
     8401 atcgggacca gtttttgcga gtgtgtcttc ttgtctttca gcagaaattc ggccagcagt
     8461 ccgttttcat cagctaccgc tataccggcc gttgcagtgg acgtatcaac cgcaagcgct
     8521 ctcatcagta agcctcctct cgtattccgc agtcctgtca ccgatgaaac tgacggtgat
     8581 cgtcctgctg tcggagccct caggatcatt tttttctata tagacctcta tacgctctga
     8641 aggaagtatt tctcttatga ggtccgccca ttctatgacc gtcactccgt ctccgtcgat
     8701 gtattcataa tatcctgtgt cgagcatctc atccacatcg ccgatcctgt acacatcaaa
     8761 atggtataaa ggcagccgtc cttcatactc attgacgatg gtgaaagttg gactggttat
     8821 gtatccgctg atcccgaggc cttttgcgat accgcttgta aaagcggtct tgcctgtccc
     8881 aagatcaccg aaaaggcaca cgttgtcgcc cctttgcagc atactgccaa gttttcttcc
     8941 cagttcaacc gtatcagcga cagaactggc gcggaattcc ttcatatcct ttttaccacc
     9001 tgaccgttta taatcgtgta aagcaccctg gatttcagtt ccagcggatg tccgtccatt
     9061 attatgatat cggcatcctt gccggcctca aggcttccta ccctgtctgc tatccccgtg
     9121 atttcagcgg catttatagt aatcgcctta agtgcttcag tttcgtccat cccctctttg
     9181 accgcgaccg ctgcgcaaag cggaaggtac tgtacggggg tgaccggatg gtccgtcatt
     9241 atggctgttt tgattcccgc tctcgacagt attcccggcg tcctggcgct ttgattcctg
     9301 agttctatct tcgaccggtc ggtcaaaaac ggcccggtga tgacacttac attctcctcg
     9361 agcaaaatat ccgtgatcag gtgaccttcc gtgcaatgct cgatcgtgat gccgaccccg
     9421 aactccttag cgatccttat cgcggtcaaa atgtcgtcag cccggtgggc atgggcttta
     9481 agcgggatct ccccgttgag gaccttcagc agcgcttcca ttttaatatc ataatcaggc
     9541 ttgtcaaaat tctcgctgtc ccgctcatag tcttccttca tctttttata ttcgacagcc
     9601 ttcatcaggc tttccctgag cagcgcagcc gtggccatcc ttgtcatggg cgcctgccgt
     9661 ttttcgtgat atacgctttt aggattttct ccaaatgcaa ctttcatggc cacaggcgcc
     9721 tttatgacca tatcctcgac cctgcgcccg tacgtcttca gcgctgcgaa ctgcccgccg
     9781 atcacattgg cactgcccgg gcctgtaaca acggtggtga cgccgttctc tcttgcctcg
     9841 acgaaagccc ggtccgcgtg gtaaacagca tcgatagccc gcaggtgcgg catgacagga
     9901 tctgaggtct catttccgtc gtcgccttcg aatccgacgg aatcctccca catgcccaca
     9961 tggcagtgcg catccacgaa tcccggcaaa acatagcgtc cctttgcatc tatgacttca
    10021 gacgccgccg cttccagcct tgccagttct gcagcgtcat tgcccagcgc ggttattttc
    10081 tccccttccg cggcaatgta tccgtcattg tagtcaatac ctgccattgt gagtattttc
    10141 gcgtttttta caagcagcat gcaaagacct cctgttgttg attcatagct tacttatcat
    10201 ataattccgt aatatatcca attacctggt atttgccgta ttgcctttca tggttgagga
    10261 ctgcgttacg ctactcattc atcattcaat atcatcacaa aaccatgtcc tgatatccat
    10321 aatataatat cactgtttac tgcattttaa aatattgacc ataagctata ttgccaagag
    10381 caaaataact gtccaaccag attagataca tctatcggta tcggattttt cttatatggg
    10441 attttttaat ggaagttaag tctcataaaa tgatttatga atttttaagg attgcatttc
    10501 ttataggaac taactcgttg cactaatata atatatttcc gcctgcagaa atactaattc
    10561 cacaaatgag gtttgcttac tgtaaaatac aagcattatc tattctttcg caacatacca
    10621 ggtatttgcc attattaaac aatagctgga atatttattg cctcctaaat atcccatctt
    10681 tacagctttt tgcgcatgac aagccttatt tttgtttggc ttttactttt ttataaatag
    10741 tgatgatgaa agacattatg ccaaaaactg ttaaaaacaa atatatcgat aaaggaaaat
    10801 accattgcac aaactctttt ccggcatccg gattgccgtg tgctttttga atgtaacaaa
    10861 acaggatcat tgatatgaga gaaagcacgg gaacaatgca gctcaaaaca attttagttt
    10921 tttcccattt tgaaatatat gatgtcaata atactaaaaa gagtattccg gtcaacaggc
    10981 ttaattcaaa agtaaataaa atcataaaag atttcccctt aatttatgat caatataata
    11041 atatattgtt tcacataatt ttacaactat gatatgaaca tgcattttta ttttactatt
    11101 cttttgcgac aaagtttgcc cgtttgcaca aaaaaaccgc caaaggtcaa tagccaatga
    11161 cggtttttaa tctcgaaaga aaactttgca ggtttgaaaa aacccgggta taaattatca
    11221 gagatattac ctcttgctga actgaggcgc ccttcttgcc ttcttgagac cgtacttctt
    11281 tctttctttc atcctcgggt ctcttgtcag gaaacctgcc tttttcaatg caggtctgaa
    11341 ttcttcgtca gccttgacga gcgcccttgc gatgccgtgc cttatagcgc cggcctggcc
    11401 tgacaggccg cctccgacga cattgcacaa aacatcgaat ttgcccaatg tttcggtaag
    11461 taccaacggc tgctttacga taactttaag ggtctcaagc ccgaaataat catctatgtt
    11521 cctgtcatta acggttattt tgccctctcc gggtacaagc ctgactctgg cgatcgattt
    11581 ctttcttctt cctgttccgt aatactgtat tttagctgcc atcgttatac tcctccctcc
    11641 tcatcaaaca tcgatttcga gtacttccgg cttctgagcc tgatgctcat gctccgggcc
    11701 cctgtagact ctgagtttcc tgaacattgc acgcccgaga ctgttgtgag ggagcatacg
    11761 cttaactgca agctcgactg ccttttcagg cgaattctcc atcaactgcc tgtacttggt
    11821 ctctttcagc ccgcccggcc agccggaatg acgcctgtaa accttctgat ccagcttctt
    11881 tccggtcagg acggcctttt cggcgttgat tacgatcaca tagtctcctg tatcaacgtg
    11941 aggcgtatat ataggcttgt gctttcccct cagaatgctt gcaactacgc ttgcaagtct
    12001 tcccaacggc ttgccggcgg catcgactac gtaccatttt ttcttgactt cttcagcctt
    12061 tgccatataa gtcttcatct gttacctttc ctccctgatt acttcgtata tatgttcaaa
    12121 ccggcctttg acacagctca aaacatgccg gtctttcagg cactttacta taataataca
    12181 ggtcctccgc acatgtcaag tgaaaaaccg ataaatttaa tttatctttc attatctctt
    12241 gattatagcc ttaatatacc catatctccc ttataatcgt ccgtcatttc acttttttga
    12301 atctgctctg catccggttc ttcctgtcag atcctacgtc ccggtcccat tcccccggtt
    12361 tggaaccatt ttaagatgcc aatgtcccag tccggtaact ctcttctgtc tcatcttacc
    12421 tgtctgaagt tctcttcatt cccgctatct ctgcttaatg cggcgatctg tcacaaacgc
    12481 cgcagcctac cctgcaattg tatcaaaaat tacgctaata atcaccttca tatcgcaaaa
    12541 ttagccgtaa aaaaggacca gggctggccc tggtcctgta acg
//
LOCUS       DAOSCC010000044        12539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3390_length_12539_cov_4.471403, whole genome shotgun sequence.
ACCESSION   DAOSCC010000044 DAOSCC010000000
VERSION     DAOSCC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_12539_cov_4.471403"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLG72_03840"
     CDS             complement(<1..718)
                     /locus_tag="PLG72_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79962.1"
                     /translation="MFKVFLVEDEIVVREGIRNNIDWEQYGFQFAGDAPDGELALPLI
                     RQIKPDVLITDIKMPFMDGLALIELVRRELPKTRIIIISGYDDFSYAQQAIRMGVEQY
                     LLKPIAKSKMVEVLTELHRKMVEEQKQQEYFEEFMREVREYKIFTHRRFFEKIVTGGL
                     TVQEITKTARELEIDLNAPFYDIVLFSLSSAGYDGSAPERYTEVLAAVEEEIREYISG
                     HPELILFIWNVTIYAVLVKGG"
     gene            complement(891..2171)
                     /locus_tag="PLG72_03845"
     CDS             complement(891..2171)
                     /locus_tag="PLG72_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ79963.1"
                     /translation="MKTSKKNTLKSILRKNELFRTLAEVKGNPRACILTEPMWGLSMN
                     LCLPYATVYMLTFGMSDIQVGIVTSVYMFSQMICAFLSGAIIDKMGRRKSTAIFDFLA
                     WSVPCIIWASSQGFWFFVVAALFNGMMKITTVSWDCLLVEDAPKDRITHIYSWVLICG
                     NLSALFAPISSLLVSKLTLAPAIRILYLNAYVVMTAKLLILYKFSTETAVGRARREAA
                     RGKSWGEMLSGYKNALNKIIKSQGTIFAIMVSILVEIALMIGATFWQIIVSRRIGVPD
                     TLLPIFPMVKSMLSIFLFFTVISKIRQTRLKWPMYGGFISYMISCALLVSITGTGVMG
                     YVLLTISIFFEALGSSVMSTLRESLVAIHADPEERSGVLALLQTTVMLVSVPFGYIGG
                     LLSDISRTLPFVLNIGLMLLGILATAKYGARHVS"
     gene            complement(2559..3920)
                     /locus_tag="PLG72_03850"
     CDS             complement(2559..3920)
                     /locus_tag="PLG72_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOJ79964.1"
                     /translation="MANRFSRITPEIMELSSLFENNSRIDPSLYDKYDVKRGLRDING
                     KGVLTGLTVISEIVSSKTVDGKEVPCEGKLYYRGIDVEEIVEGFIKEDRFGFEEVAYL
                     LLFGELPDAERLESFGRILSNYRTLPTNFVRDIIMKAPSHDMMNTLARSVLTMYSYDD
                     NADDNSIPNVLRQSLQLIALFPLLAVYGYQAYCHYHDQKSLFIHRPQSELSTAENILY
                     ILRPDSKYTPLEAKILDLALVLHAEHGGGNNSTFTTHVVTSSGTDTYSAITAALCSLK
                     GPRHGGANIKVVQMFEDMKARISDWTDEGQVADYLERILEKKEFDRSGLIYGMGHAVY
                     SLSDPRATVLKKFVESLSREKDRMDEFRLYSLVERLAPEIIREKRKIYKGVSANVDFY
                     SGFVYNMLGLPLQLFTPLFAIARLPGWAAHRMEEIANAGKIIRPAYKSVSERRQYIPL
                     DQR"
     gene            complement(4319..5146)
                     /locus_tag="PLG72_03855"
     CDS             complement(4319..5146)
                     /locus_tag="PLG72_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HOJ79965.1"
                     /translation="MKYDVELVGKIGSMALIREKEHDIDYNVFSRIGSELRPGMIWVS
                     SGAVEIGRLDYIKRTGHELTGPKEDIMTDYSAQGQTILMEEYRRYIPSKYSVRQLLVE
                     HVHFNDPEKRDHIYRFLMRCVKQDAIPIVNYNDTVSFEENRRWELEQLRSNGRREIVE
                     CIDNDETAAVIATLVHPKYLLIFTSVEGIYTDPKDPSTLVRCVEGKNADEVIEAINEL
                     QKHCDGASRSGAGGARSKLEFIKEPIRQGTTVMIASSRYRISDVLSGNCPRTVFRVR"
     gene            complement(5386..6474)
                     /locus_tag="PLG72_03860"
     CDS             complement(5386..6474)
                     /locus_tag="PLG72_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ79966.1"
                     /translation="MKKATASRPGYIGKRLAVLIPAVLILTGLLLPLPGCMPKKAVGS
                     SAGDNEDNVPKIGFIYETMTVQRWQRDRDIFVAKAQNLGAEVIVKNAYEDSELQKEIA
                     IEMINQGVDVLVIVAYDKDSLKDLVKYAHNRDVKVIAYDRIIRNVDIDFYISFDNTEV
                     GRLMGKAAVEKVPEGNYLILNGTERDNNSFWFNEGYYSVLKPYIEKGAISIVGETWVD
                     DWRSESSYDFVSKILNSGQKVDAIIAANDQLAEGAVTALSENRLAGEVFVTGQDAELG
                     ACRRIVEGTQGCTVYKPINVLAEGAAELAVKLAEGKDISVYETISNGDYSIPCKIYKP
                     ILVTKDNIDETVIQDGFFTAEQVYRNAP"
     gene            complement(6636..8246)
                     /locus_tag="PLG72_03865"
     CDS             complement(6636..8246)
                     /locus_tag="PLG72_03865"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79967.1"
                     /translation="MYSLMVCDDEQIMIESVRHIVEKEFSNIRIIATARSGREAIEKT
                     LTIKPDIILTDIRMPGINGLEAIKEIKKVHKDVKIIIISVYEFFEYAKQAVELGVSEY
                     LIKPVKKERLVEVLQRIVHQLDDERRKYRWELEAKERIEKMLSAVEHSFIYSLLFAQS
                     QRTDISRYKKEFFDIDNDTGFIFVLMFRQKGKKGDYDAQLENSFDNEEIYTLFKNNLK
                     YRCKCIIGPVMLDRVVVYVAQSVDDLYQQRVQAISCIEEIMDLLGKKYDIEFKVGIGR
                     IHDDQDILLSYQEALKALNCDEGGRIVHIDDIAPNTSDAGYEIDLLEQKLMISLENGD
                     VQRCLTILSDIFRKYPSVFEQDSIHYRIIEMMAAVRRIAMENGIRDKTDPGHYIKRIL
                     CCCSREEFEQVCIEEVRNIACQISARKKSNIGKIVELTNKIINERFSEDLNLDDVSKE
                     LHISPQYLSRLYKNETGENFIERLTSVRIENAKKLLKEYTYSIKEVCYMSGYSDPNYF
                     SKLFKKHVGLSPTDYQKQVQGGFQRGLD"
     gene            complement(8224..9780)
                     /locus_tag="PLG72_03870"
     CDS             complement(8224..9780)
                     /locus_tag="PLG72_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ79968.1"
                     /translation="MGKLWNRMFNSIQTRIIAVFVLTMALTSAASIFVLGLSRNIIGK
                     MDEMFEANVEIENFLNDLHTVDAHLVNYLVTSDSDSLLYYYRYKEIFSEKVENMFSEL
                     HGIYDQNDLIYKDIKYMAQSYFNEADAAVGAKMIDNADEYIARYAEANRINSYIQMYA
                     GRLNLNILDKNTTQYLGMSENLSKLHTANLVLTISVILLNVLVIAYMTYNMTKPIIKL
                     AHSAEEISRGNFDAEDIEVVSQDELSIMANAFNAMKHSIRNYIRELHNKADTEAKLLE
                     QQIENLRMQSLLADAEMKALQMQINPHFLFNTLNAGVQLATLEGADRTSAFLDSMAKI
                     LRYNVKSLDRTVRIKDEIDTIKAYEDLFMVRFGDSIRFEYNIDRNLLGIKVPPLIIQP
                     LVENATIHGIGNMENGGVIRISLERCGDIARIIVEDNGAGMDEDTRQKILKCQPFGCD
                     ESGHMTGIGIYNVVQRLRLFFNCEDVVDVESAPCQGTKVVLKIPCDADNGLDPIGTEG
                     VVTGVQPHGV"
     gene            complement(9970..10980)
                     /locus_tag="PLG72_03875"
     CDS             complement(9970..10980)
                     /locus_tag="PLG72_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ79969.1"
                     /translation="MKWLYKISTALLFATAVASAALAMYYLNKANEAYNGNTVDVFSG
                     NPEYHFSLIIHTGDDEEYWQSFKEGVFEAGKVHNAAIEYNPISDPDSTDKIIEYVNIA
                     AKSRVDGIIVNGRNSEEYSEAVNSAAESGVHIVVGAAETVDGNRISYVGTNFYDYGEK
                     AAKLISQASDKGFPIDLAVILSDESRNGISQSDIMMSGIKSVIEKEKRINLLCTLYRS
                     SDLLGAEDLTRDILNQHPDVDVIFCTNAKDTEAAARVIVERNLVGDVVIVGTGLTEEI
                     RHYIRKGIIFGVLDRNGYDAGYKAVETLCGSMGYSFHSDYIYIDTDIYTQINIDQGDK
                     KR"
     gene            complement(11238..12461)
                     /gene="gguB"
                     /locus_tag="PLG72_03880"
     CDS             complement(11238..12461)
                     /gene="gguB"
                     /locus_tag="PLG72_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ79970.1"
                     /translation="MNKFKDSMDRTLRQYGMIIALVAIIVFFVFMTGGRLIWPRNVSM
                     LVRQNAYVLILAIGMMFCILTGGNVDLSVGSLVALVSAMSGLTLTTLGLPSWLAILIS
                     LLTGLLAGIWQGFWIAYVKVPPFIATLSGMLVFRGIDNIILDGQTLPLSGLYTTIGTG
                     SVPDIVPDTIPDFLHIGDLLQLQNKPEYLNATMLFTGLVLSVIYVLAVIFSRQSKKRR
                     GYEVQPLGQTIAKIIAFFIAINLFSYWLAMDDGLPIMLILLVALYAIYTFIANKTVLG
                     RHIYAMGGNIKAAELSGVNTKKMMFLVYANMGLLAGVAGIVTAGRLMAASPNVGQGFE
                     LDAIGACFIGGASAYGGVGTIWGAVVGAFIMGVMNNGMSIMGLDVFLQSVAKGLVVLF
                     AVAFDMYSKSKSRLG"
BASE COUNT         2961 a         3357 c         2734 g         3487 t
ORIGIN      
        1 ttccgccctt aacaagcacg gcgtatattg tgacgttcca tatgaacagg atcagttccg
       61 gatgacctga tatgtactcc ctgatctctt cctccactgc ggccagcact tccgtatacc
      121 tctcaggcgc gcttccgtca taaccggcac tgctcagaga aaagagcacg atatcgtaga
      181 atggcgcatt cagatctatt tccagctccc tggcggtctt ggttatctcc tgcacagtca
      241 gtccgccggt gacgattttc tcaaaaaatc tcctgtgcgt aaagatcttg tattcgcgca
      301 cctcgcgcat gaactcctcg aagtattcct gctgcttctg ttcctcgacc atcttccggt
      361 gcagttctgt caggacttcc accatcttgc tcttcgcgat cggcttgaga aggtattgct
      421 cgactcccat gcggatagcc tgctgtgcat aggaaaaatc atcataaccg ctgattatga
      481 tgatcctggt cttcggaagt tccctgcgca caagttcgat aagcgcaagc ccgtccataa
      541 aaggcatttt gatatcggtg atcaggacat caggctttat ctggcggatc aggggcaggg
      601 ccagctctcc gtcaggggca tcgccggcaa actggaagcc atactgctcc caatcgatat
      661 tattgcgtat gccttcacgc acaactatct catcttcgac aagaaagact ttgaacatat
      721 cgtctcccct tccggcagac gcagccatga aatggtagca agttatatta tataatatcc
      781 ggtgccgatt tgaaataggc tgacagaaat aaggcgccag gcgcattggc agaaatgagg
      841 cgttatcagc accggcagct cgggacggtc attcctcaat ggttggctgt ctaactgaca
      901 tgcctggcgc cgtactttgc agtcgcaaga atcccgagca gcatcaggcc aatgttcagt
      961 acaaacggca atgttctgga gatatcgctc aacagcccgc ctatatatcc aaagggcaca
     1021 ctgaccagca tgacagtcgt ctggagcagg gcaaggaccc cggaacgctc ttcggggtcg
     1081 gcatggatgg ccaccagtga ttccctcagt gtgctcatga cggatgagcc cagcgcttcg
     1141 aagaatatcg aaattgtcag cagcacatat cccatcaccc ctgtgcccgt gatcgaaaca
     1201 agaagcgcac agctgatcat ataactgatg aaaccgccat acatcggcca tttcagcctt
     1261 gtctgcctta ttttcgatat caccgtgaag aaaagaaaga tcgaaagcat gctcttcacc
     1321 attgggaaaa tgggcagcaa tgtgtccgga acgcctatgc gccgtgatac gatgatctgc
     1381 cagaaagtcg ctccgatcat caatgcgatc tctacaagta tgctgaccat tatcgcaaaa
     1441 atcgtgcctt gtgattttat gattttattg agcgcatttt tatacccgga cagcatctct
     1501 ccccacgact tgccgcgcgc agcttcgcgc ctggctcttc ctacggcagt ttccgttgaa
     1561 aacttataca ggatcagcag ttttgccgtc ataacgacat aagcattcag atacagtatc
     1621 cgtattgcag gagccagtgt cagtttagaa acaagcaacg acgatatcgg ggcaaacaac
     1681 gcggaaaggt tgccgcagat gaggacccag gaatatatat gggttatccg gtcctttggg
     1741 gcatcctcaa caagcagaca gtcccaggac actgttgtta tcttcatcat accgttgaac
     1801 agcgcggcca cgacgaagaa ccagaacccc tggctggatg cccatatgat gcatgggacg
     1861 ctccatgcca gaaaatcaaa aattgcggtg ctcttcctcc ggcccatttt gtctatgatc
     1921 gcacctgaca ggaatgcaca gatcatctgg gaaaacatat agacagaggt aacgatcccg
     1981 acctgtatat cgctcattcc gaaagtgagc atatataccg tcgcgtatgg aaggcacagg
     2041 ttcatcgaca gcccccacat cggctctgta agtatgcatg ccctgggatt cccttttact
     2101 tcggcaagag tacggaataa ctcgttttta cgcaaaatac ttttcaatgt gtttttcttt
     2161 gaagttttca ttgtttttct ccccggtatt tctgaagtca ctttctctcc ggtatcttgc
     2221 aattgcttac attgtccctg aaattatata ccggcatttc tgtaaatgca tacgcttttg
     2281 gcatcatatt cccaggctgc cttagcagct gcattcgctc cagtatctct aaaaacaggt
     2341 acacataacc aaaaaagata cacttacgaa acaaaccgcc ggaaatcggc ggtttgcttt
     2401 actgtattaa cttaatatga ttccatacat ataggatttt agcttatcgc gtcagctaca
     2461 cctgcttgta actttcagag ttagctatac atacggattt gcgatgtttt cagccggtcc
     2521 gtgcatgcct gcagcatcgt acatcctttg acgccggatc acctttggtc aaggggaata
     2581 tactgccgcc tttctgaaac cgatttgtaa gccggcctga tgatcttgcc tgcattcgct
     2641 atctcctcca tacggtgggc tgcccacccg ggtaaacggg ctattgcaaa caggggcgta
     2701 aacaactgca atggcaatcc aagcatgttg tatacgaaac cgctgtaaaa atccacattt
     2761 gcgctgacac ccttgtagat tttccgcttc tcccgtatga tctcaggagc cagcctttca
     2821 acaagtgaat aaagcctgaa ctcatccatg cggtcctttt ctcttgaaag gctttccacg
     2881 aatttcttga gtacggtcgc acggggatcg gacagcgagt aaacggcatg gcccatcccg
     2941 tatatcagcc ctgaacggtc gaattctttc ttttccagga tccgctccag gtagtccgcg
     3001 acctgacctt catcggtcca gtcggatatc ctggccttca tatcttcaaa catctgcact
     3061 accttgatat tcgcaccgcc gtgcctgggt cccttcagcg agcaaagcgc cgctgtgatg
     3121 gccgaataag tgtctgtccc ggaagaagtc accacatggg tggtgaacgt ggagttgttg
     3181 ccgccgccat gctcggcatg caataccagc gccaggtcga gtatcttggc ttccagaggc
     3241 gtgtacttac tgtccggcct taaaatatac aatatgttct cggcagtcga aagttcagac
     3301 tggggccggt gtatgaaaag gctcttctga tcatggtaat ggcaataagc ctggtatccg
     3361 tagacagcca gcagcgggaa caaagcgatc aactgcagcg actgccgcag cacgttgggt
     3421 atcgaattgt catcggcatt gtcatcatag gaatacatcg tcagaacgct tctggccagc
     3481 gtgttcatca tatcatggct gggagccttc ataatgatgt cccgcacgaa attcgtcggc
     3541 agtgtccggt agtttgacag tatcctgccg aaactctcaa gccgctccgc atcaggcaac
     3601 tcgccgaata acagcaggta agccacttct tcaaagccga aacggtcttc tttgataaaa
     3661 ccttccacga tctcttcaac gtcgattccc ctgtaataaa gtttcccttc acagggaact
     3721 tctttgccgt cgaccgtctt tgacgaaacg atctcagata tgactgtcaa tcccgtcagt
     3781 acgcctttcc cgtttatatc ccgcaaacct ctttttacgt catatttatc gtaaagtgag
     3841 ggatcgatcc tgctgttatt ttcaaataaa gaggaaagtt ccattatctc gggggtgatc
     3901 ctggagaaac ggttagccat tatgaagtca ctcctttcac ttttttatga ctgtttttta
     3961 attatacaat aacatcggcc cgatggaaag gatgaaaacc ctaaactgcc ggaaatgccg
     4021 caatcccggc gcctgctgtc agcaataata atataggtat cgggctctcc ggtttatata
     4081 aacatatttg tataaactgt atataaactc aatgaaacat ctggttgtgt aaacagacgg
     4141 cttcctggcg gtatttgact ataatacgcc gttatggttt atgatgtctt tccggcaata
     4201 gcagataaaa atgtctcagt attgtattac tgacgccgac cggcaggaca caggactcaa
     4261 ggccaaatgg caatcaggat ggcatacccg gtacacagat aatggaccgc gtctgtcgtt
     4321 atcttacccg gaaaacggtc ctcgggcaat tgccggaaag gacgtcggaa atcctgtatc
     4381 gtgaactggc aatcataact gttgtaccct gccggatcgg ttcctttatg aattcgagtt
     4441 ttgatctggc tccgccggcg ccggaacggc tcgctccgtc gcaatgcttc tgcagttcgt
     4501 ttattgcttc tatcacttca tctgcatttt tgccttccac gcatctgacc agtgtggacg
     4561 ggtccttcgg gtcagtatat atcccctcca cggaagtaaa aatgagcagg tactttggat
     4621 ggaccaatgt tgctatcact gcggccgttt catcgttgtc gatgcattcc actatttccc
     4681 tgcggccatt actgcgaagc tgctcaagtt cccagcgcct gttttcctca aaactgacgg
     4741 tgtcattgta attcactatc ggtatggcat cctgttttac gcaacgcatc aggaaccggt
     4801 atatatgatc ccgcttttcc ggatcattga aatgcacgtg ttccaccagc agttgcctga
     4861 cagagtattt cgacgggatg taccggcgat actcttccat cagtatcgtt tgtccctggg
     4921 cggaataatc ggtcattatg tcttctttcg ggccggtcag ttcatgccct gtgcgcttga
     4981 tataatccag gcgccctatc tccactgcgc ctgaacttac ccatatcatt cccgggcgca
     5041 gttcgctgcc tatacggctg aaaacattgt aatcgatatc atgttctttc tcgcggatca
     5101 acgccatgga ccctatcttt ccaaccaatt ccacatcata tttcatgata cattaacccc
     5161 tgtcagatta ttcattttaa ataatacaga ataaatagcc tgataatgca aatcaagcag
     5221 taaatgcggt tttaatatac cagttctgct ccggtttttc cggttttgat ttctatgccg
     5281 gtcatattcc ggtcttatac ctggcagtac caactgtctt acattttatt tcgatacggg
     5341 gaaaatttca agcgtgaact gccaaacagc cgaccgatcg gactttcacg gtgcattcct
     5401 gtatacctgc tcagcggtga aaaatccgtc ctgtatcacc gtttcgtcta tattgtcctt
     5461 tgtaacaagg atcggtttgt atatcttgca gggtatacta taatcaccgt tggatattgt
     5521 ctcgtatacg ctgatatcct tgccttccgc cagtttcaca gccaattcgg cagccccctc
     5581 ggcaaggacg tttataggct tgtaaaccgt acatccctgt gtgccttcca ctatcctccg
     5641 gcaggcgcca agttcggcat cctgccccgt aacgaacact tcacctgcaa gcctgttttc
     5701 actcaaagca gttacagcgc cttcagccag ctggtcattt gcagcaatga tggcatctac
     5761 cttctgcccg ctgttcagga tcttgcttac gaaatcatag gaactttccg agcgccagtc
     5821 atcgacccag gtttcaccca cgatgctgat ggcgcccttt tcgatatatg gtttgagtac
     5881 actgtaataa ccttcgttga accaaaagga gttattgtcc cgctccgttc cattaagaat
     5941 gagataattc ccttcaggta ccttttcaac ggcagctttg cccatcagac gtcctacttc
     6001 agtgttgtcg aacgatatgt agaagtctat atcgacgttt cttattatgc ggtcatatgc
     6061 gatgaccttg acatcgcggt tatgggcata tttgacaaga tccttgaggc tgtccttatc
     6121 gtaagcgact attacgagca catccactcc ctggttaatc atttcgatcg cgatctcttt
     6181 ctggagttca ctgtcctcat aagcgttttt tacaataact tcagcgccaa ggttctgcgc
     6241 ctttgccaca aatatatccc tgtccctctg ccacctttgg actgtcatcg tttcatagat
     6301 gaaccctatt ttcggcacat tatcttcatt gtcgccggca ctgctgccaa cggctttttt
     6361 cggcatacag ccgggcagag gcagcagaag tcctgtcagg ataagcacgg ccggaatcaa
     6421 cacagccagc cgcttcccaa tatatcctgg tctggacgcg gtcgcctttt tcatggcaaa
     6481 ttcctcccgg atgccgtaat atataccatt atagcttttg aactgtatat gtgtcaaaat
     6541 gctttaattc tgtaattgtt tggctgcaaa aatccttgga tcttcacaac caaaatacat
     6601 ttcaggcaca tccctgcagt tacagtcgcc gcggctcaat ccaggccacg ctggaaccct
     6661 ccctggacct gcttctgata gtcggtcggg gacaggccga catgtttctt gaacagtttg
     6721 ctgaagtaat tcgggtcgct gtatccggac atatagcata cttccttgat ggaatacgtg
     6781 tattccttca gcagtttctt tgcgttttct atccggactg acgtcagccg ttcgatgaag
     6841 ttctcaccgg tctcgttctt atacagcctg ctcagatact gcggactgat gtgcagttcc
     6901 ttcgaaacat cgtccaggtt caggtcctcg ctgaaccttt cgtttatgat tttgttagtc
     6961 aactcgacta tcttcccgat attgcttttc ttcctcgcgc ttatctggca ggctatgttc
     7021 ctgacctctt cgatacatac ctgctcgaat tcctccctgg aacaacagca taatatcctc
     7081 ttgatgtagt gccccggatc cgttttgtcc ctgatgccgt tttccatggc aatgcgccgt
     7141 accgccgcca tcatttcgat gatcctgtag tggatgctgt cctgttcgaa aacactggga
     7201 tacttccgga agatatcgga caaaatcgtg aggcaccttt gcacatcgcc gttttccagg
     7261 gatatcatca gcttctgctc cagcagatcg atctcgtatc cggcatcgct tgtgttggga
     7321 gctatatcat ctatatgcac tatcctgccg ccctcatcgc aattgagcgc cttcagcgcc
     7381 tcctggtaag acagcaggat gtcctggtca tcgtggatcc ttcctatccc gaccttgaac
     7441 tcgatatcat atttctttcc cagcaggtcc atgatctcct ctatgcagga gatcgcctgc
     7501 actctttgct gatacaggtc atcgacgctt tgggcgacat atacgacgac cctgtccaac
     7561 attacaggac ctattatgca tttgcacctg tacttgagat tgttcttgaa aagagtatag
     7621 atctcttcat tgtcgaagct gttttccagt tgggcgtcgt aatcgccttt tttgcctttt
     7681 tgtctgaaca tcagcacgaa aatgaagcct gtatcgttat caatatcgaa gaattccttc
     7741 ttatatctgc tgatatctgt tctctgcgac tgggcaaaaa gcagggaata tatgaagctg
     7801 tgttccacgg ctgagagcat cttttcgatc ctctccttgg cttcaagctc ccatctgtat
     7861 ttccgcctct catcatccaa ctggtgaacg atcctttgca ggacttcaac cagcctctct
     7921 tttttcaccg gttttataag gtactcgctc actcccagtt cgacggcctg tttggcatac
     7981 tcgaaaaatt cgtatacaga tatgattatg attttcacgt ccttgtggac cttttttatc
     8041 tccttgatcg cttccaggcc gtttataccg ggcattctaa tatccgtcag tattatatcc
     8101 ggcttgatcg tcaaagtctt ttcgatcgct tccctcccgg atcttgcagt cgcgatgatc
     8161 cggatattgc tgaactcttt ttctactatg tgccggacgg attctatcat gatctgttca
     8221 tcatcacaca ccatgaggct gtacacctgt aaccacccct tctgttccga ttgggtccaa
     8281 cccgttatcc gcatcacatg gtatcttcaa aacaactttc gtaccctggc agggagcact
     8341 ctcgacatca acgacatctt cacagttgaa aaacagcctt agcctctgta ccacgttgta
     8401 tattcctatc ccggtcatat ggccggattc gtcgcatcca aacggttggc acttcaggat
     8461 cttctgcctt gtatcctcat ccatcccggc tccgttgtcc tcgacgatta tcctggcgat
     8521 atcgccgcat ctttccagcg atatccttat cacgccgccg ttctccatgt tgcctatacc
     8581 gtggatcgtt gcattctcga caagaggctg gataatcagg ggcggcacct tgatgccgag
     8641 aaggttccgg tcgatgttgt actcaaatct gatcgaatca ccgaaacgca ccataaacag
     8701 atcctcatac gcttttatgg tatcgatctc gtcttttatc ctcacagtcc tgtccaggct
     8761 cttgacattg tatctcaata tctttgccat actgtcgaga aaggccgatg tcctgtccgc
     8821 cccctccagc gttgcaagct ggactcccgc attgagggtg ttgaaaagga aatgcgggtt
     8881 tatctgcatc tgcagtgcct tcatttcagc gtccgcgagc agtgactgca tcctcaggtt
     8941 ctctatctgc tgttccagca gttttgcctc ggtatctgcc ttgttgtgca gttccctgat
     9001 gtaattcctt atgctgtgct tcatcgcgtt gaatgcattg gccatgatgc tgagctcatc
     9061 ctgggaaacc acctcgatat cttcagcatc gaaattgccc cgggagatct cttcggccga
     9121 atgcgcgagt ttgatgatcg gcttcgtcat attataggtc atatacgcga tgacaagcac
     9181 attcagcagg atcacggata tcgtaaggac caggtttgcc gtgtggagct tgctgagatt
     9241 ctccgacatg ccaaggtatt gggtggtatt tttatccagg atgttcaagt tgagccggcc
     9301 ggcatacatc tgtatgtaac tgtttatcct gttcgcttcg gcgtacctgg caatatactc
     9361 atccgcgttg tctatcattt tggcgccgac ggccgcgtcc gcttcattga agtaggattg
     9421 ggccatgtac ttgatatcct tgtagatcag gtcattctgg tcgtatatcc cgtgcagttc
     9481 ggaaaacatg ttctccactt tttcggaaaa gatctccttg tacctgtaat aatagagcag
     9541 gctgtccgaa tctgatgtca cgaggtaatt cacgagatgg gcatccacgg tatgcaagtc
     9601 attcagaaaa ttttcgatct caacattcgc ttcgaacatc tcatccattt tcccgatgat
     9661 gttcctcgac agtcccagca cgaaaatgct tgcagcgctg gtcagagcca ttgtaagcac
     9721 gaaaactgcg attatccttg tttggatact attgaacatc ctgttccata atttccccat
     9781 ggtcattaac cccaactctc acgccatttt gaaaatacgg ccgtgtatcc gccatgcagg
     9841 ggcatttccc cgaatatgcc gccgtgtttc cgtcaaatgc ggcggcgttt cccttcgggt
     9901 acgccactgc atccccgcca taacagccgc gcctccaatc gtcctcagca ttgcgcccgg
     9961 ccgtatgctt caccgttttt tgtcaccctg atcgatgttt atttgcgtgt atatgtccgt
    10021 atcgatatat atataatcag aatgaaatga ataccccatg ctgccgcata gtgtctccac
    10081 ggctttatag cccgcatcgt aaccgtttct gtccaggact ccgaatatga tccccttccg
    10141 gatataatgc ctgatctctt cagtcagacc agtgccgact atgacgacgt cccccaccag
    10201 gttccgctca acgatgacac gtgcagctgc ttccgtatcc ttggcattcg tgcagaagat
    10261 aacgtccaca tccggatgct ggttcagtat atcccttgta aggtcttccg cccccagaag
    10321 atcactgctt ctgtaaagcg tgcaaaggag gttgatcctt ttttcctttt ctattacgct
    10381 ttttataccg ctcatcatga tatcgctctg ggatatgcca ttccggctct cgtccgacag
    10441 tatcacggca agatcgatgg gaaatccctt gtcggatgcc tgggatatca gttttgcggc
    10501 tttttctcca tagtcgtaga aattggtccc gacatacgag atcctgttgc cgtctacggt
    10561 ttccgccgcg cccaccacga tgtggacccc tgattcagcc gcgctgttga cagcttcgga
    10621 gtattcttcc gaattcctgc cgttgacgat tatcccgtcg accctggatt tggctgcaat
    10681 attcacatat tcgatgatct tgtctgtgct gtccgggtcg gatatgggat tgtactcgat
    10741 tgcagcatta tgtaccttcc cggcttcaaa aacgccttcc ttgaagctct gccagtactc
    10801 ttcatcgtct ccggtatgaa ttatcaatga gaagtggtat tccgggttcc cgctgaatac
    10861 atccacggtg ttgccgttgt aagcctcatt tgccttgttg agatagtaca tcgccaatgc
    10921 tgcagatgcg accgcagtgg caaacagaag ggcagtcgat atcttgtaca gccatttcat
    10981 ttgaatgccc cctcaagcat ggtacatcat gctgaagttc attactatta taacatactc
    11041 gttacgatga tgcctgtctt gcagtccgga tccgtatatc agcaactgca tccctgtcac
    11101 cgggactata ccgttaaaag acaaaggggc gtccggatcg attcggatca aatcgaatcg
    11161 gatcataata atcaaaccga ttcggtccaa agcggatctg atcctgaaac agccccgtat
    11221 cacatttcct gtcgagttca gccgagcctg gattttgact tcgaatacat gtcgaatgcg
    11281 actgcaaaaa gtaccacgag accttttgcc actgactgga ggaacacatc cagtcccatg
    11341 atcgacatgc cgttgttcat tacgcccatt atgaaagctc caacaacagc tccccatatc
    11401 gttccgacac cgccgtatgc tgaagctccg ccgatgaagc acgcgccgat agcatccagt
    11461 tcaaagccct gacctacatt cggcgatgca gccatcagcc tgcctgcggt aactatccct
    11521 gcaactccgg ctaaaagccc catgttcgca tacacaagga acatcatctt ttttgtgttg
    11581 actccggaaa gttccgccgc cttgatattt ccgcccatag catagatatg ccgtcccagt
    11641 acggtcttgt tcgctatgaa cgtatagatg gcatacaatg ctacaagcag gatgagcatt
    11701 atcggcaggc cgtcgtccat tgccagccag taggaaaaca ggttgatagc gataaagaac
    11761 gcaatgatct tggcaatggt ctggcccagc ggctgcactt catatccgcg tcttttcttg
    11821 ctttgcctgc taaagataac agccagcaca tatatgacag ataatacaag cccggtgaat
    11881 agcatcgtcg cattaagata ttcaggcttg ttttgaagct gcagcagatc accgatatgc
    11941 aggaaatcag ggatagtatc cggaacaata tcagggaccg agcccgtccc gatggtggta
    12001 tacaggcccg aaagcggcaa agtctgtcca tcgagtatga tattgtcgat cccgcggaaa
    12061 acaagcatgc ctgatagtgt cgcaataaaa ggaggcacct tgacatatgc tatccagaac
    12121 ccctgccaga tacctgccag aaggcctgtc agcaggctga tcagtatcgc cagccacgaa
    12181 gggaggccaa gggtcgttaa cgtcaacccc gacatggcgc ttacgagtgc gaccagcgaa
    12241 ccgaccgaaa gatcgacatt accgcccgtc agtatacaga acatcatacc tatggcaaga
    12301 atgagcacat aagcgttctg gcggaccaac atcgaaacgt ttctcggcca gatgagcctg
    12361 ccgcctgtca tgaacacgaa gaatacaata atggcaacca gtgcgattat catgccgtat
    12421 tgccgcaaag tgcgatccat ggaatctttg aatttgttca taagtttccc ccctatcgtc
    12481 cggccgttgt gttaatctat tttaaatatc aatagattca aatgttttat ttgtaaggg
//
LOCUS       DAOSCC010000045        12530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3397_length_12530_cov_4.162485, whole genome shotgun sequence.
ACCESSION   DAOSCC010000045 DAOSCC010000000
VERSION     DAOSCC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3397_length_12530_cov_4.162485"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLG72_03885"
     CDS             complement(<1..584)
                     /locus_tag="PLG72_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ79971.1"
                     /translation="MKERLMTRKPVKPFLSLIRMQLNVNYGISALKYRFTREKKKRWE
                     PVLIGLAIVVGLVPLLAMYTFFMMSVFAGGQIMGQPEIVLTSAFVFAQLVILIFGILY
                     IMGSFYFSNDVMSLVPLPLKPYQIIGAKFAVVLVNEYLTSVPLLLPPVIIFGIGTSQG
                     LLYWLKSIVLMAFAPVIPLAVASLLVILVMRVVN"
     gene            complement(581..1303)
                     /locus_tag="PLG72_03890"
     CDS             complement(581..1303)
                     /locus_tag="PLG72_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ79972.1"
                     /translation="MIEIRNLSKSYNKGQVKAVDGLDLKVNRGEIFGFLGPNGAGKTT
                     TIKMIVGLLNPDTGTITIDGTDISKEPLEAKRKIGYVPDEPVLYERLTGLEYLNFMAD
                     VYQVPASERKERIEYFLEMFNLRDAAADLIKSYSHGMRQKTALAGALLHDPKLWILDE
                     PMVGLDPKSAHLLKNQMREHCDKGNTVFFSTHVLEVAEKLCDRVAIINRGKLIAIGTV
                     DELRHGDQKDSLEKIFLELTEQ"
     gene            complement(1710..2786)
                     /locus_tag="PLG72_03895"
     CDS             complement(1710..2786)
                     /locus_tag="PLG72_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ79973.1"
                     /translation="MKSYRTIFIWIGIFAVIGIVLLMVACETGVLTGQNGQEQIDEEQ
                     QDVQDGDISEDIGESRENQKAGTDQQPATSDEPERLTVGKPYSEKLVEDGARNILFIG
                     QDKVSNLYDTMGIVSVDSKNKKVKIIMIPRDTYIEYNDKILDELEKANKLKVPGYLKI
                     NNTHHVGGIINYSGKFDSGPISFLADVIGEKFNVEIHDYVKINIKGFRELIDHLGGID
                     IKVPYRMDYKDPVQDLVIDLQPGYQHLDGHDAEGFVRFRQGYKEDGTWFEIGDFERKK
                     NQLYFIRELIKQKGTLKNIDKIPGIIKILGKNIQHSIGIGDVLTSYIGMATDVITSKY
                     EIVTETLYSDKMIRVYGSSYIVLE"
     gene            complement(3561..5309)
                     /locus_tag="PLG72_03900"
     CDS             complement(3561..5309)
                     /locus_tag="PLG72_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOJ79974.1"
                     /translation="MEMVNITIDTRKIQVPKNYTVLEAAKYANIEIPTLCFLKDINEI
                     GACRMCVVEIKGARSLQAACVYPVSEGLEIYTQTPRVREARKVTLELILSNHEKKCLT
                     CVRSRNCELQKLAEDLNIKDIRFEGEMIHHPIDDLSPSIVRDPNKCVLCRRCVNMCKN
                     VQQVGVIDTNERGFRTIVGSAFEKSLAEVPCINCGQCINVCPVGALRERDDTDLVWEA
                     LHNKDIHVVVQTAPAVRAALGEEFGMPIGTRCTSNMIAALRRLGFRKVFDTDTAADLT
                     IMEEVTELVNRIKNGGKLPLITSCSPGWIKFCEHNYPEFLDNLSTCKSPHQMFGAVLK
                     SYYAEKTGIDPSKIFVVSVMPCTAKKFESKRPEMTATGYPDVDVVITTRELARMIKEA
                     GIDFTELPERHFDDPMGEATGAGVIFGATGGVMEAALRYAVELLNGKPLEKVEFEAVR
                     GVEGIKEATIEAGGMKIKAAVASGTGNAKKLLDRIKNGEAEYHFVEIMACPGGCVNGG
                     GQPIQPSSVRSWTDLRTERAKALYEEDENMPLRKSQDNPFVKKLYEEFLGHPGSHKAH
                     ELLHTHYAPRENYPED"
     gene            complement(5334..7130)
                     /gene="nuoF"
                     /locus_tag="PLG72_03905"
     CDS             complement(5334..7130)
                     /gene="nuoF"
                     /locus_tag="PLG72_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOJ79975.1"
                     /translation="MDFYRAHVLICGGTGCTSSNSQKVKQELEAQINKNNLDKEVKVV
                     TTGCFGLCAQGPIMVVYPEGVMYTMVKVEDVEEIVNEHLIKGRPVKRLMAGDKEADDT
                     TKSLENVDFFKRQVRIALRNCGIINPENIDEYIAYDGYKALAKALTEMTPDQVIEEVK
                     KSGLRGRGGAGFPTGLKWELTKKSKSDKKYVVCNADEGDPGAFMDRSILEGDPHSVIE
                     AMTIAGYAIGADQGFIYVRAEYPIAVKRLQIAIKQAQEYGLLGRNIFGTDFSFDLELR
                     LGAGAFVCGEETALITSIEGHRGEPKPKPPFPAVSGLWGKPTLINNVETYANIPVILN
                     KGADWFSKIGSEKSKGTKVFAVGGKINNTGLVEIPIGTTLREVIYDIGGGIPNGKKFK
                     AAQTGGPSGGCIPASHIDTPIDYETLRELGSMMGSGGLIIMDEDTCMVDIAKFFLEFT
                     VDESCGKCPPCRIGTKRMLEILEKITSGKGEMEDIDKLEMLAKNIISSALCGLGQSAP
                     QPVLSTLRYFRDEYVAHVRDKKCPAGVCKSMLSYVIHADRCRSCGICARNCPVGAISG
                     EKKQPYVIDQEKCIKCGACMEKCPFKAIFKNA"
     gene            complement(7164..7541)
                     /locus_tag="PLG72_03910"
     CDS             complement(7164..7541)
                     /locus_tag="PLG72_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ79976.1"
                     /translation="MKSLAELEEIRKKTLDEIKMRKNKEGIRVVVGMATCGIAAGARP
                     VMNRFVEEIAKRKLSDVSVTMTGCIGVCKLEPIVEVINADGTKVTYVKMTPEKASRVV
                     AEHIVNGRVCQDLTIGAAEQAGK"
     gene            complement(7525..8091)
                     /locus_tag="PLG72_03915"
     CDS             complement(7525..8091)
                     /locus_tag="PLG72_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ79977.1"
                     /translation="MKEIALHILDIMQNSVAAGAGRIGVSITADDETGMITAVIDDNG
                     CGMDEKMLQRVTDPFTTTRTTRRVGLGIPLLKSSCIMSGGSFGIKSQKGCGTRVTAAF
                     GKDHIDRPVLGDLAGVIADMSASFPEIDIRLELVHGEKRFSYSSADAGRILGEVPLSE
                     PAVVKWLKEYIDEGVKDIFGGVLNEIIG"
     gene            complement(8119..8613)
                     /gene="nuoE"
                     /locus_tag="PLG72_03920"
     CDS             complement(8119..8613)
                     /gene="nuoE"
                     /locus_tag="PLG72_03920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOJ79978.1"
                     /translation="MSSNNCCCGCVDEKERKLQEIIDKYKDTKGALIPVLHEVQELYG
                     YLPMSVQKKISEGLGVPLSEIYGVVTFYAQFYLKPKGRFRINVCLGTACYVKGSNLIL
                     EKLQEKLGIHVGDCTEDGKFSLEACRCVGACGLAPVIMINDDVYGRLTPDDVEGVLEK
                     YKDL"
     gene            complement(9299..10012)
                     /locus_tag="PLG72_03925"
     CDS             complement(9299..10012)
                     /locus_tag="PLG72_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ79979.1"
                     /translation="MNYAVDLHIHSALSPCADKDMTPNNIVNMAVLKGLDAIAVTDHN
                     SAANLQTIGICAQKCGLVFIPGMEVETSEEIHIVCLMPDMERMSRFHDAVRQAMPRLD
                     NRPDIFGRQLIMDENDNIVGEEQMMLLTASRLTVDDVIGLARKLDGAAIPAHVDRSSY
                     SIISNIGFVPEEFGIRYLEISRDCDESMFRSMHPELDRYQFVRSSDAHYLGDILEREN
                     MLELDELSPEAIIRRLACR"
     gene            complement(10267..10599)
                     /locus_tag="PLG72_03930"
     CDS             complement(10267..10599)
                     /locus_tag="PLG72_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ79980.1"
                     /translation="MRVAELAARLDAKIITGDNGTDREIKGVYCCDLLSWVMSHADKG
                     SAWITVHTHMNIVAVAVLTELACIIIPEGIEAEEATVRKAAEECIPILSTGLTAYEVC
                     CVAHDCGI"
     gene            complement(10647..11951)
                     /locus_tag="PLG72_03935"
     CDS             complement(10647..11951)
                     /locus_tag="PLG72_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ79981.1"
                     /translation="MGEYFHSVTLDEEKCKGCTNCIKRCPTEAIRVRHGKARIINERC
                     IDCGECIRICPYHAKKAITDDLSIISNYKYKIAIPAPSIYGQFKPVYSRNRILNAFYR
                     IGFDDVYEVAMAAEYVSDATRKLLSEEGTRKPLISSACPAVVRLIQVRFPNLIDNLIK
                     LESPMEVAAKMVKKKTAEKKGIDPSDIGVFFITPCAAKATSVKAPYENKKSSVDGAIS
                     IKSIYLKLLANLDKADDTVQLAKAGFEGIRWANSGGEGLAVDTDNFLAVDGIHNVISI
                     LEAVENDKIEGIDFIEALACTGGCLGGPLTVENVYVAKTRLKKYVEAAKEAQKGIRQP
                     LEPYDESIVWTGEIAYKPVLKLDTDIDKAIKKLEALEKINDGLPGLDCGACGAPSCRA
                     LAEDIVRGIAYETDCIFKLREKVRDLAAQMKELEEKAPPTMG"
     gene            complement(12034..12453)
                     /locus_tag="PLG72_03940"
     CDS             complement(12034..12453)
                     /locus_tag="PLG72_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOJ79982.1"
                     /translation="MDLHYEIPGDDLAHAGEASSNVKKILVQLGIDPVKVKKAAISMY
                     EAEINAVIHGGGGSADVAIDSDKIIIRIIDSGPGIEDLELAMQEGYTTASDEIREMGF
                     GAGMGLPNMKRYADELDIQTAKDKGTVVTIVVYFHQQ"
BASE COUNT         2643 a         3450 c         2771 g         3666 t
ORIGIN      
        1 atattgacga ctctcatgac cagaattacc agcaatgaag ccactgcaag ggggatcacc
       61 ggcgcaaaag ccatcaggac gatgcttttc agccagtaca gcagcccctg tgaggtcccg
      121 atgccgaata tgataaccgg cggcagcagt aaaggtaccg atgtaagata ctcattgacc
      181 aggaccacgg cgaacttggc gccgattatc tggtacggct tcagcggaag cggtacgaga
      241 ctcatgacat catttgagaa gtagaacgag cccattatgt agagtatgcc gaaaataagg
      301 atgaccaact gggcaaatac gaaagccgat gtcagcacta tctccggctg gcccataatc
      361 tgcccgcctg cgaataccga catcatgaag aacgtataca tggccagcag cggcaccagc
      421 ccaactacta tggccaatcc tatcagcaca ggttcccagc gttttttctt ttcccttgtg
      481 aacctgtact tcagggcaga aatgccgtaa ttgacattca gctgcatcct gatcagtgac
      541 aaaaacggtt ttaccggttt gcgcgtcatc aatctttcct tcattgctct gtcaactcca
      601 ggaatatttt ttcaagcgaa tccttctgat caccatgcct gagttcgtca acagttccta
      661 tagcgatcag ttttcccctg ttgatgatag ccaccctgtc gcacagtttc tccgccactt
      721 ccaggacatg cgtcgagaaa aatacggtat tgcctttgtc acagtgctca cgcatctggt
      781 tcttgagcag gtgtgccgac tttggatcaa ggccgaccat cggctcgtca agtatccaca
      841 gcttcgggtc atggagcagc gcaccggcca gcgccgtttt ctgcctcatt ccgtgggaat
      901 agctcttgat caggtccgca gccgcatccc tgagattgaa catctccagg aagtactcga
      961 tcctctcttt tctctccgat gcaggcacct ggtatacatc tgccatgaaa ttcagatatt
     1021 caagacctgt caggcgctcg taaagtacag gttcgtcagg tacataccct attttccttt
     1081 tagcctccag gggctctttt gaaatgtccg tgccatcgat ggtgatggtt ccggtgtcag
     1141 ggttcaaaag gccgactatc attttgatcg tcgtggtttt ccccgcgccg ttgggcccga
     1201 gaaagccgaa tatctcaccc ctgttgactt tcagatccag cccgtccaca gctttgacct
     1261 gacccttgtt gtaactcttt gacaaattgc ggatctcaat catgtaccat acctcctgca
     1321 aaagatgcac tgccaagtac caagcactgc ggcggtcccc gcattatatt tttccgcttc
     1381 ggcacgcgcc tgatctgctt atgccgttta ctttcggcat caagcgcctg caaagcgctc
     1441 gtttgtcagt cgcaggcatt acctgcctct cagtctttaa catatcccta agttatatta
     1501 ccatattgtt tccaaccatg tcattatacg gattgaacaa gaattttgta tgccacggct
     1561 tgtatgacat aatacctgaa agtaaacctg ggtaattcag gaaatatcac gcaaaaaaat
     1621 accggttccc tccgctgcga tacatggagt tttgcaccgg cactttccag gcactgaata
     1681 tgaagtttca gatctgatag tatggagtat cactcgagca cgatatatga cgatccgtag
     1741 accctgatca tcttatcgct gtatagtgtt tcggttacta tttcatattt actcgttata
     1801 acgtcagtcg ccatccctat atatgatgta agaacatccc caatgccaat actgtgctgt
     1861 atattcttgc caagtatctt tattatcccg ggtattttat cgatattttt cagagtgccc
     1921 ttctgcttta tcagttcccg tatgaaataa agctggttct ttttcctctc gaaatcacct
     1981 atttcaaacc acgtaccgtc ttccttgtat ccctgcctga agcgtacaaa gccctcggca
     2041 tcgtggccgt ccaggtgctg gtagcctggc tgcagatcta tgaccaggtc ctggaccggg
     2101 tccttgtagt ccatcctgta tggcactttt atatcgatgc ctccaagatg gtcgatcagt
     2161 tccctgaacc ccttgatgtt gatcttcaca taatcatgga tctctacgtt aaacttctcc
     2221 ccgatcacat cggcaagaaa gctgattggt cctgaatcaa acttgccgct gtaattaatg
     2281 atgccgccca catgatgtgt attgtttatc ttcaggtatc cgggcacctt gagcttgttg
     2341 gctttttcaa gttcatccag tattttatcg ttgtattcta tatatgtatc cctggggatc
     2401 attatgatct ttactttttt attcttgctg tcgacactga ctatgcccat cgtgtcatac
     2461 aggttactca ctttgtcctg gccaatgaag aggatgttcc tggcgccgtc ttccacgagt
     2521 ttttcagaat agggcttgcc tacggtcagc ctttcaggct catccgaagt ggcgggctgc
     2581 tggtctgtac cggccttctg attttcccgg ctctcaccga tatcttcgct gatgtccccg
     2641 tcctggacat cctgctgctc ctcatcaatt tgctcctgcc cattctggcc tgtgagcact
     2701 ccggtttcac aggcgaccat gagcagcacg atgccgatca ctgcgaatat cccgatccat
     2761 atgaaaatgg tcctgtaact cttcatcagc ggttctcccc gtcaaatcat tctttgctcg
     2821 ctggagccag tcgcggaaac gatctcattc gcatataatc caaatattac gtataccttt
     2881 tatattataa tgcggtttcc ttcatgattc aaataaatag ttgttaaaat attgttaaag
     2941 cgacaggcct tgtaagtatt tatcttacag gataacgggc cttatacaga gagtggaagg
     3001 cttttattta gtcttataca gaatacgccg ggtttcatcc ggacatgcaa aaaccaggag
     3061 aagccgtcaa gttccggccg ctcctggttt ttactgaaga cgtatcgatt ctgctgcttg
     3121 atccgctgct ccgtgtattg ccttatttat ttcccctgtt gcttccctgt gcgttgcttc
     3181 atatgtgcca taaatcctgt tgtttatata cctgtctatt ctcctgaaaa tgctccgaaa
     3241 attgctgcgt aagctgctcc gctgaatgcc ctgaacatta ccttgcctgt tgcatggtat
     3301 atagcttcgc atgttgcttc actatatatt tgcacacatt tttgtacttc taatcatttt
     3361 tttaataaat cttagtgcct tccggctgcc ggcatactaa aaggggcagt ccaaaaggtt
     3421 ttcgacctga tggacgcccc ttcttcagtt actgttccat ccgaattatg tggaccagca
     3481 tttcaactgt atcgtttgtg taacagccaa atcgatcaac ttagctgcct cacgatcgga
     3541 tcactgtccc atcgcaatca tcagtcctcg ggataattct ccctcggcgc atagtgcgtg
     3601 tggagcaatt cgtgagcctt gtggcttccc ggatgtccaa ggaattcctc atacagcttc
     3661 tttacgaacg ggttgtcctg ggacttcctg agcggcatgt tctcgtcttc ttcgtacagg
     3721 gcctttgccc tttctgtcct gaggtcggtc cagcttctga ccgaagaagg ctgtatgggc
     3781 tggccgccgc cgttgacgca gccgccgggg cacgccatta tctccacgaa gtggtactcc
     3841 gcctcgccgt tctttatcct gtcgagcagt ttcttcgcat ttccggtgcc gcttgcgacc
     3901 gcagccttta tcttcatgcc gccggcttcg atggtggctt ccttgatgcc ttcgacgccc
     3961 cttacggctt cgaactccac tttctcgaga ggcttgccgt taagcagctc tacggcatat
     4021 ctcagtgccg cttccataac gccgcctgtc gcgccgaata ttacgccggc gcctgttgct
     4081 tctcccatcg ggtcgtcgaa gtgcctttcc ggcagttcgg tgaagtctat gccagcttcc
     4141 tttatcatcc tcgccagttc tctcgtggtg atgaccacgt cgacgtccgg gtacccggtg
     4201 gcagtcattt cgggcctctt ggactcgaac ttcttggctg tgcagggcat gaccgacact
     4261 acgaatatct tcgacggatc tatgcccgtc ttctctgcat aatacgactt cagcactgcg
     4321 ccgaacatct ggtgcggcga cttgcaggtc gaaaggttgt ccaggaattc cgggtaattg
     4381 tgttcacaga atttgatcca gccagggctg catgatgtga tcagcggcag cttcccaccg
     4441 ttctttatcc tgttgaccag ttccgtgacc tcttccatta tggtgagatc cgcagccgtg
     4501 tctgtatcga atactttcct gaagccaaga cgcctcaaag cagctatcat gttgcttgtg
     4561 caccttgtgc ctatcggcat tccgaattct tctcccaggg cggctcttac cgccggagcc
     4621 gtctgtacaa cgacatggat gtccttgttg tgcagcgctt cccataccag gtcggtatcg
     4681 tcacgctctc tcagggcgcc caccgggcag acattgatgc actgtccgca attgatgcag
     4741 ggcacctctg caagagattt ctcgaatgcc gaaccgacta tcgtcctgaa acctctttca
     4801 ttggtatcga tgacccctac ctgctgtacg ttcttgcaca tattcacgca gcgcctgcag
     4861 aggacgcatt tgttgggatc ccttactatt gagggtgaaa ggtcgtctat cggatgatgt
     4921 atcatctcgc cctcgaacct gatgtccttt atgttgaggt cttcggcaag cttctgcagt
     4981 tcacagttcc tgctcctgac acaggtaaga cactttttct catgattcga aaggatgagt
     5041 tccagcgtta cctttcttgc ttccctgacc ctcggggtct gagtatatat ctcaagccct
     5101 tccgatacgg gatacacgca tgccgcctga aggctcctgg cccccttgat ctcgaccaca
     5161 cacatcctgc aggcgccgat ctcatttatg tccttcagga agcaaagtgt cggtatttct
     5221 atgttggcat attttgcagc ctccaaaacg gtgtaattct tggggacctg gatttttcgg
     5281 gtatctatcg taatattgac catttccatc tcttcagcac tctcctttct acatcatgcg
     5341 ttcttgaata ttgccttgaa cggacatttt tccatacaag caccacactt gatacatttc
     5401 tcctggtcta tcacgtatgg ctgcttcttc tcgccgctga tagcgccgac tgggcagttc
     5461 ctcgcgcaga tgccgcagct tctgcaccgg tctgcatgta tcacgtagct cagcatggac
     5521 ttgcagacgc ccgcaggaca tttcttgtcc ctgacgtgtg cgacgtactc gtccctgaaa
     5581 tacctcagcg tgctgagcac gggctgcgga gccgactgtc caagcccgca cagcgcggag
     5641 gatatgatgt tcttagccag catctccagc ttgtcgatgt cctccatttc gcccttgccg
     5701 cttgtgatct tctcaaggat ctcaagcatc ctcttcgtac cgatacggca cggcgggcat
     5761 tttccgcagg attcgtcaac tgtgaactcg aggaagaact ttgcgatatc caccatacag
     5821 gtgtcctcgt ccatgatgat gagtccgcct gaacccatca ttgaaccgag ttccctcagt
     5881 gtctcgtaat ctatgggagt atcgatatgg cttgccggta tgcatccgcc tgaaggcccg
     5941 ccggtctggg cagccttgaa tttctttccg ttgggtatac cgccgccgat atcgtatatg
     6001 acttctctca gtgtggtacc gatcggtatt tcaacaagac ccgtgttgtt gatttttccg
     6061 ccgactgcga acaccttcgt gcccttactc ttctcggagc ctatctttga gaaccagtct
     6121 gcgcccttgt tcaggatcac gggaatgttt gcataggtct caacattgtt gatgagcgtg
     6181 ggtttgcccc agagaccgct gacagccggg aacggcggct tcggcttcgg ttcgcccctg
     6241 tggccttcaa tggatgtgat cagcgccgtt tcctcgccgc acacgaacgc gcctgctccg
     6301 agcctcagtt ccaggtcaaa actgaaatcg gtcccgaaga tgttcctgcc gagcagtccg
     6361 tactcctggg cctgttttat ggctatctgg agccttttaa cggcgatagg atattcggcc
     6421 ctgacataga tgaatccctg gtctgcgcca atggcataac ccgctatggt cattgcctcg
     6481 atcacgctgt gaggatcgcc ctcgagtatg cttctgtcca tgaacgcgcc ggggtcgcct
     6541 tcatcagcat tacagaccac gtatttcttg tcgctctttg actttttagt cagttcccac
     6601 ttcaggccgg tcgggaaccc tgcgccgcct cttcccctga ggcctgattt cttgacttct
     6661 tcaatgacct gatcaggggt catttccgtc aatgcctttg ccagcgcttt atatccgtca
     6721 tatgctatat attcgtcgat attctcaggg ttgatgatac cgcagttcct gagcgctatc
     6781 ctgacctggc gcttgaaaaa gtccacattt tcaagcgact tcgtcgtgtc gtcagcttcc
     6841 ttgtcgccgg ccatgagcct ctttaccggt ctgcccttta taagatgctc attgacgatc
     6901 tcttcgacat cctcgacctt taccatcgtg tacatgacgc cttcaggata cacgaccatg
     6961 atcggaccct gggcgcaaag cccgaagcag ccggtggtga cgaccttgac ttccttgtcc
     7021 aggttgtttt tatttatctg agcttcaagc tcctgtttga ccttctgtga atttgacgag
     7081 gtacagccgg tacctccgca aatcagcaca tgggctctgt aaaaatccat tttgctccct
     7141 cccgcaattc ttcaacaagt gttttatttc cctgcctgct cggcagctcc gatagtgaga
     7201 tcctggcaaa cccttccgtt tactatgtgc tcggcgacga ctcttgacgc tttttcaggg
     7261 gtcatcttca catatgtgac ctttgtgccg tctgcgttga ttacctccac gatgggttcg
     7321 agcttgcaaa ctccgatgca gcctgtcatg gtcaccgaaa catccgaaag ctttcttttg
     7381 gcgatctctt cgacgaatct gttcattaca ggccttgctc cggctgcgat cccgcaggtc
     7441 gccatgccta ccactacacg tatgccttcc ttgttctttc tcatctttat ctcatcaaga
     7501 gttttctttc ttatctcttc gagttcagcc aatgatttca ttcaatacac ctccgaaaat
     7561 atctttcacg ccttcatcta tatattcctt cagccatttg accaccgcag gctcggacag
     7621 aggcacttcc ccaagtatgc gccctgcatc cgctgaactg tatgagaatc gcttttctcc
     7681 atgtaccagt tccagccgga tatcgatttc cgggaaggat gcagacatgt ccgcgataac
     7741 gccggccaga tcgccgagca ccggccggtc gatgtgatcc ttcccgaatg ctgcggtgac
     7801 ccttgttccg cagcccttct gcgattttat gccgaaactt cctccggaca ttatacagga
     7861 acttttcagc aacggtatcc ctagtcctac ccttctcgtc gtcctcgttg tcgtgaacgg
     7921 atcggtcacc cgttgaagca ttttctcgtc catcccgcat ccgttatcgt ctatcactgc
     7981 agtgatcatc ccggtttcgt catccgccgt tatcgatacc ccgatcctgc ctgcgccggc
     8041 tgccactgaa ttctgcatta tatcaaggat gtgcagcgca atctctttca aatccgggat
     8101 acctcctgta taaagcattt aaaggtcttt gtatttttcc aggacgcctt caacgtcatc
     8161 aggtgtcagt cttccgtaaa cgtcgtcatt tatcattatt acaggtgcaa gaccgcatgc
     8221 tccgacacag cggcaggctt ccagcgagaa ctttccgtct tccgtgcagt ctcccacatg
     8281 tatcccaagc ttctcctgca atttttccag tatcagattt gaccccttga catagcatgc
     8341 agtgccgagg catacattga tacggaacct tccctttggc ttgagataaa actgggcata
     8401 gaacgttacg actccataaa tctctgacag cggaacaccc aggccttcag atatcttctt
     8461 ctgaacgctc atgggcagat atccgtacag ttcctgaact tcatgaagta caggtatcag
     8521 cgcccctttg gtatccttat acttgtcgat aatttcctgc agcttgcgct ccttctcgtc
     8581 gacgcatcca cagcagcagt tgttactgct catttcgcat gccctccagt ccttttgtta
     8641 tttgtttgag ttttagtgct gattcagtgt atgtccaatt tctacatttt gctcggattt
     8701 cgttctgata tatttcatat atatttcaca gtaattcaca ctttaggtat aaatcacaag
     8761 caaaaaatca tgattgtcaa cgtcacgaac caagttgtta agcttttaac ataccctgtc
     8821 attatagcaa atatgcggcc gcaattcaac tgctaattca ataattttta cggacgtttt
     8881 gtatacatta tacagttttt acgtccgcag tgccatatga gcaggtttgc tttatttttc
     8941 actttgcatc aaataattgg taaggctgat tgggaacgct gattggtaag gctcggagac
     9001 tgattggtaa atcgggattg acccagaata tgcagtcata cgttaaatat gtttcctgta
     9061 ccgttttttc gtttatgggc gctggggcaa cttttttgtt attttttgta ccgttttttc
     9121 gtttatgggc acttaaagca ttcattacca cttcacatgt ctacataaag tatatggtat
     9181 tatgtaaatc ataatacggt aaagaagtgt cttaaacttg aaaaatatgc ccataaacga
     9241 aaaaatggta cagaaaacgg ggtaaacttt aacatgtgca aaagccgccg gttcgccttc
     9301 atctgcaggc aagcctgcgt attatcgctt cagggctgag ttcgtcgagt tccagcatgt
     9361 tttccctttc aaggatatcg ccaagataat gcgcatctga agatctgaca aactgatacc
     9421 tgtcaagttc cgggtgcatc gatctgaaca tggattcgtc gcaatccctg gagatctcaa
     9481 gatacctgat gccaaattcc tcaggcacaa agccaatatt cgaaatgata ctgtatgaag
     9541 acctgtcgac gtgtgcaggg atggcagcgc cgtccagttt cctggccagg ccgatcacgt
     9601 catcgacagt caaccgggaa gccgtaagca gcatcatctg ctcttcgccg actatgttgt
     9661 cgttttcgtc cattatgagc tgcctgccga atatgtccgg ccggttatcc agccggggca
     9721 tcgcctgccg gaccgcatca tggaaacgtg acattctttc catgtcaggc atgagacata
     9781 cgatatggat ctcttcggag gtctccacct ccattccggg gataaatacg agaccgcatt
     9841 tttgcgcgca tatgccgatc gtctgaagat tcgctgccga attgtggtct gtgacagcaa
     9901 tggcatcaag acctttcagg actgccatat tgacgatgtt gttaggcgtc atatccttgt
     9961 cggcacatgg cgaaagcgcc gaatggatat gcagatccac agcataattc atctgttatc
    10021 tcccggaaga ggcctgatac tgcggcagag acacggtaac acatcataag tgcaagatta
    10081 cttcgctcag gcgatggaaa ccaaatatca cgataaaggc atgcatcgtg tagggtcctg
    10141 gttcaggcaa atcacgactt tccttcagat ctggttccgg atttgctccg ggtcaggctc
    10201 cggaactgct ttggtgaaag ttccggcatt tcccataata cagcctcgta tcggggaatg
    10261 ttcgcctcag ataccacagt cgtgtgcgac gcagcatact tcatacgcgg taagtccggt
    10321 gctcagtatc gggatgcact cctcagccgc ttttctgact gtcgcttcct ccgcttctat
    10381 accttcaggt attattatac atgctaattc tgtcaacaca gctactgcaa cgatattcat
    10441 gtgggtgtgc accgtgatcc atgccgagcc tttgtctgcg tgcgacatta cccagctcag
    10501 aaggtcgcag cagtatacac cctttatttc cctgtcggtt ccgttatctc cggtgattat
    10561 ttttgcgtcc agccttgcag ccagttctgc cactctcatg tcgtccctcc aaagcatccg
    10621 gggttctgtc cggtccatcg tgagcgtcag cccattgtgg gcggcgcctt ttcctcaagt
    10681 tctttcattt gggccgccag atcccttact ttttcacgga gcttgaatat gcagtccgtt
    10741 tcatacgcaa tgcccctgac gatgtcctcc gcaagggccc tgcagcttgg tgcgccgcat
    10801 gccccgcagt cgaggccagg cagtccgtca tttatctttt caagggcttc aagctttttt
    10861 attgccttat ctatatcggt atcgagcttc aataccggtt tgtatgcgat ctcaccggtc
    10921 cagactatgc tttcgtcgta cggctccagg ggctgcctga tgcccttctg ggcttccttt
    10981 gcggcctcga catacttctt aagccttgtt ttggccacat aaacgttctc tactgtaagc
    11041 ggccctccca ggcatcctcc agtgcaggca agcgcttcaa tgaagtcgat gccttcgatt
    11101 ttgtcgtttt cgaccgcttc caggatcgaa atgacattgt gtataccatc gacggccagg
    11161 aagttgtcag tgtctactgc caacccttcg cctcccgagt ttgcccaccg tatgccttca
    11221 aagccggctt ttgccaactg tacggtgtca tctgccttat caagattggc caggagcttc
    11281 aggtatatgc tttttataga gatcgctccg tcgacggatg atttcttgtt ctcatacgga
    11341 gctttcacac tggtggcctt tgccgcgcat ggcgttatga agaagacccc gatatctgaa
    11401 gggtcgatcc ctttcttttc ggcagtcttc tttttgacca tcttcgccgc cacttccatg
    11461 ggagattcca gcttgataag gttgtctata aggtttggga atcttacctg tatgagccgc
    11521 acgactgcag gacatgctga tgaaataagg ggcttcctgg tcccttcttc cgaaagcagc
    11581 tttctcgttg catcagacac atattctgcc gccatcgcaa cttcgtacac atcgtcgaat
    11641 ccgatcctgt agaatgcgtt cagtatcctg ttcctgctgt ataccggctt gaactgtccg
    11701 tatatgcttg gagcaggtat tgctatcttg tatttgtaat tgctgattat cgacaaatcg
    11761 tccgttatcg cttttttcgc atggtaagga cagatcctga tgcactcgcc gcagtctatg
    11821 catcgttcat ttatgatgcg ggccttgcca tgccttactc tgatcgcttc cgtggggcat
    11881 ctttttatgc agtttgtgca gcctttgcat ttttcttcgt ccagtgttac ggaatgaaag
    11941 tattctccca taatcccacc tcttcagtac agcaatccct tcaggcaact gaccgcaagt
    12001 tgatctgaac agaccgctgc cgttgctgta gctttactgc tggtggaaat acaccactat
    12061 cgtgacaacg gttcccttgt ctttcgctgt ctgtatgtcg agctcgtcgg cataccgctt
    12121 catgttgggc agtcccatgc ctgctccgaa tcccatttcc cttatctcat ccgatgctgt
    12181 cgtatagccc tcctgcatgg ccagttcaag atcttcgata cctgggccgc tgtcaatgat
    12241 gcggatgatt attttgtcgc tgtcgatggc gacatcggca ctgcctccgc ctccatggat
    12301 gactgcattt atctctgctt cgtacatcga tattgcagcc tttttgacct tgacgggatc
    12361 gatgcccagt tgtaccagga tcttctttac gttgctggaa gcttctcccg catgcgcaag
    12421 atcgtccccc ggtatttcgt aatgcagatc catcctgctt ttttccaacg gtcagacacc
    12481 ctttcctgta agtcctgcgc tgtacagctt gccgcaggcg ataaacatgg
//
LOCUS       DAOSCC010000046        12453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3429_length_12453_cov_4.601468, whole genome shotgun sequence.
ACCESSION   DAOSCC010000046 DAOSCC010000000
VERSION     DAOSCC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_12453_cov_4.601468"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2103)
                     /locus_tag="PLG72_03945"
     CDS             complement(<1..2103)
                     /locus_tag="PLG72_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ79983.1"
                     /translation="MRYGYFDDANREYVITTPETPSPWINYSGNQEFFSLISNTAGGY
                     CFYKDARLRRITRYRYNNVPVDSGGRYFYIREENGEFWSPTWAPVRKKLDKYECRHGL
                     GYTRIYGERGGIGGEVLIFVPQDFNGEVHKVTISNKGSQARKIKLFSFIEWCLWNAYD
                     DMTNFQRNFSTGEVEYESNTIYHKTEYRERRNHYAFYSANTAVDGFDTDRYEFIGQYN
                     GFDAPQVVVEGKSRNSMADGWSPIASHCFDITLQPGAQKEIVFVLGYVENDPEDKWEG
                     NNIINKEKAKKMIEKFSTPRQVDDALGELKQYWDGLLSRYVVDSGDEKLDRMVNIWNQ
                     YQCMITFNMSRSASYFESGIGRGMGFRDSNQDILGFVHQVPQRARERILDLAATQLED
                     GGAYHQYQPLTKKGNNEIGGNFNDDPLWLILSTAAYIKETGDFNILEEMVPYDNDPFR
                     AATLFDHLHRSFYHVVNNLGPHGLPLIGRADWNDCLNLNCFSTEPDESFQTTTSKDGK
                     VAESVLIAGMFVYIGNEYVKICENIGEYEEAKAAKEHIRKMKENILKYGYDGEWFLRA
                     YNDFGEKVGSRENEEGKIFIETQGFCVMAGVGVETGEAQKALDSAWKYLDTEHGMVLV
                     NPAYTRYHVELGEISSYPPGYKENAGIFCHNNPWIMCAETVLGRGDKAFELYRKICPA
                     YREEISDVHRMEPYVYSQM"
     gene            complement(2288..3865)
                     /locus_tag="PLG72_03950"
     CDS             complement(2288..3865)
                     /locus_tag="PLG72_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ79984.1"
                     /translation="MRSMKRYLCLALVLVVALSLILTGCGDKQTTDTGSGSGTSTDTG
                     AGTSTSSGSDSSGDAAATPQFKDTVELTWTLVGDKQETDTDLVMQEVNKYLADSLNIK
                     LNLQIFGWGDPYDQKVNTMLASGEPFDICFTANWAANYYKNAAAGYFLPLNDYLKKYP
                     AIVEICGQDFLNGSAINGTHYAVPTNKEKVHNWGYLFMKELVDKYNFDLSSVKSMEDV
                     EPFFETIKANEPDITPLLVVGGDSPFQFLDWDRVSDDNVPGALYPDNRNNTIVNHFLA
                     PESIDMYKKLREYFQKGWIHPDAATMENTAEQMKTGKFFAAESSLKPGKDAEMTGSTG
                     IEWVQVDITRPVMSNRETTGALLAIPAASKNPERAFLFIEKLYTDAKLRNMINFGIEG
                     THYNVNADGRITQTQAGVDRYNLGPGWRYGDQFKDLLLDNEDLDKWEKFKKFNDEGLV
                     LNSLGFVFSDESVSTESSACKTVVGTYSRQLMCGAVDVDSTVAKFEQELKASGVDKLI
                     AEEQRQYDEWRKAVGKE"
     gene            complement(3941..4855)
                     /locus_tag="PLG72_03955"
     CDS             complement(3941..4855)
                     /locus_tag="PLG72_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ79985.1"
                     /translation="MSKFMKKYRVNEIKTSTNLLINLFFIIVSVTCIAPVLLVIAISV
                     TSEQSIRQYGYQFIPSEFSTEAYDYLLRAGDAIWRAYGVSIFVTIVGTILSLAVITLY
                     AYPLSRSSFKYRYQFSFFAFFTMIFGGGLVPWYIVYTQLVPIKDSIWIMIVPYLMNTW
                     YMMIMRTFYKTTVPESIIESARIDGAGEFRTYFTIVLPLCKAGLATVGLFCTLGYWND
                     FWLPMVFVNNAKNFNIQYLMYQTLIAVQNLLNNSNQISNMTEILANLPSESLRMAIAV
                     ISIGPIVLAYPFFQKYFVKGLTIGAVKG"
     gene            complement(4866..5840)
                     /locus_tag="PLG72_03960"
     CDS             complement(4866..5840)
                     /locus_tag="PLG72_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ79986.1"
                     /translation="MDLAVSSAKTIKTSKKKGFFYQLRHQWPLFLMLLPGVVILIINN
                     YIPMFGVIIAFKRYRFHGTFWKSLVKSEWVGFKNFEFFMKTPYAWQMTRNTLLYNFTF
                     IILGLIIPVACAIALTEIRNKRLSKIYQSVAFLPYFLSWIVVSYLAYSMLSIENGFIN
                     KSILAPLGIAPVEWYSEPKYWPFILVFFQLWKYTGYNIVVYIAAITGIDPEYYEAAAI
                     DGASKWQQIKHITIPSLQTLMIILTLLAVGRIFNADFGLFYNVPRNSGTLQPATQVID
                     TYVYNALRNTNDIGMSSAAGTYQAVVGCLTVLTANLIVRKIDKESALF"
     assembly_gap    6066..6075
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6076..7657)
                     /locus_tag="PLG72_03965"
     CDS             complement(<6076..7657)
                     /locus_tag="PLG72_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79987.1"
                     /translation="MYKVLIVDDEPIIRKGLRNIINWKNFGCEVVAEAGDGLDGLELI
                     RKHKPDIIITDIKMPETDGLSMIKQMKEDVPDSKIIILTGHRDFDYVHEALKLGVTDF
                     LLKPSRIEELTSAISRAVKELKFQNQRSEEFDKLSQLFTQNISVLREKLLYDIIYEIN
                     TNRDDIKEKLELFQTRQGDFRMLLVQNDAEESDGSDISQYDRHLYQFGIINTFIEIFS
                     DKFDVLHVTLDTVGIAFIVMPREGVEDELAETINKKCTYLQEIINNCFGFTVTIAISS
                     EGSDFTELPQKFGECRAALEHKFYLGNNSIIYHSDVNTFFRYDDHSMLEKLEKALLEG
                     IKSGNETAVEGRLKDIYSYIRNIDPSQKEFIKNFYWNTITSINNIRLSLMSGEDGRKV
                     EYTELSGLYGLIEKCGNADELHTLLEESARSVVSKVNNYNNKSIKLILRKAVEYLQTH
                     YHEPITLNEVAEHTFVSTYYISRMFKKEMGKNFVDYLNELRIEKAKELLKDVRYKTYE
                     VAEKVGIPDAHYFSRLFKK"
     gene            complement(7632..9473)
                     /locus_tag="PLG72_03970"
     CDS             complement(7632..9473)
                     /locus_tag="PLG72_03970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ79988.1"
                     /translation="MKKSHRGLFRIFRKISIKTKLVLLLTTQMLIPLVLIGFLSYKNS
                     EGIIKENSTDYSRDILHMIRLRLDDYMDNLVQISQDLLYEEKIYEVLKSGTNIEDPLK
                     LYEIQNSVNSHLKMVVISRPEIRSICIYANDGRIFYQDDNTREAGTHDDLPFAEMLEQ
                     ARGKKGKPSLYIVSDGSRVQDIYMIRQINDRDDFVELGLLIMHVKKEILDEVYQGLTG
                     NFENFIILSPDMELIASRDYDDLSYMSGVLNESIDGYMGEKVDEKAGMFISYITQELT
                     GWKIVSYVSLDVLYEDANTLKNNIIMLCIAAVAVLSVVTLITAVNIVKPINKLVNGMK
                     KVQSGEGNVRIEVDREDEIGFLQKAFNEMSGEIHHLVNWVYREQLTRKEAELKALQSQ
                     INPHFLFNTLEAINWMAQLNNVPEISNTVSDLSDLMEASIGRDDRLITIEEEFKYADK
                     YISLQRRRFGDKIELIKDIRPDVYGIQIPRLLIQPLIENAVYHGIERNRGRGTIRLNA
                     YKDGKGCVCVEVIDNGAGMEPEELEELNARLAMDNDTYFKNLGGKKRTSIGIENVNRR
                     IKLFYGEEYGLKIESRVGEFTKVTITIPCKVNLDNREGFYVQGSDSR"
     gene            complement(9538..10860)
                     /locus_tag="PLG72_03975"
     CDS             complement(9538..10860)
                     /locus_tag="PLG72_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ79989.1"
                     /translation="MKILRSLTALLLVMSMAAMSGACTKTTPDLSDSDWKIVPSEDSD
                     ITLRFICSWGGVDSKAETLKLVLSQFENRNPDITVVNESLFGEDFLPKIKTDFASGND
                     PDVFGLWPGSDIKKLVTVGKVADLTGLLTDDPEWKRQFNPDMWSYTTYNERIYGLPFE
                     IIFECMFVNRDLFKKYNVKIPENFDELKEAVFIFNRNNIIPIAFNTEAEGTYFYQNLV
                     PVVGGKDGTEVPFTEGRKIKQSHIVALNLIKELYDIGAFPKECFTMTNNERNVLFKEK
                     KAAMIVQGSWYIGDFEPDDDTVDIIPFPYVKGYESNKNTMVYGLGGGTFHMSQAAWDD
                     EKKREASVRLLRFLTSPESAAQFASRTGMISTVSLEGYDIDYIDMTIKGMRMLEEADT
                     LVGPLDSYVDRTAWEKYIAAQFPYFLSGKITAEEMWEAAIENGIILDY"
     gene            complement(10946..>12453)
                     /locus_tag="PLG72_03980"
     CDS             complement(10946..>12453)
                     /locus_tag="PLG72_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOJ79990.1"
                     /translation="MNAANILKPALSRGEIQIIGATTLNEYRKHIEKDSALERRFQPV
                     LVDEPSVGEAIEILKGIRGYYEEFHKVRIPDDVIEAAVRMSERYITDRFLPDKAIDVI
                     DEAGSRANLKNVTLVELEELKSELKKVQELKESAVSADSIEDYQKAADLKVRECKLLQ
                     QIQELEQKNKETVLTVDDVAHVIESWTKIPVQRLTEVEAEKLINLEERLHKRVIGQHE
                     AVSSVAKAIRRHRAGFRKKKKPASFIFVGPTGVGKTELVRALAAELFESEDALIRLDM
                     SEYMEKHSVSKLIGSPPGYVGYDDAGQLTEKVRRKPFSVILLDEIEKAHPDVFNILLQ
                     ILEDGRLTDSHGRVVNFENTVIIMTSNAGTSLKSNGIGFNRDGYSDLRSKVMDVIKET
                     FRPEFLNRLDEIIVFTELNRDELMRIINLMLDEVTAEAEQKGISISYTDKAREFIIEK
                     GYDPKYGARPLRRTIQRYIEDELTEQFFLGNIKPGDRITVDADESGIRLVK"
BASE COUNT         2619 a         3236 c         2923 g         3665 t
ORIGIN      
        1 catctgcgag tacacatacg gttccatcct gtgcacatcg cttatttcct ccctgtaagc
       61 cgggcatatc ttcctgtaca gctcgaaggc cttgtcgccc ctgccgagca ccgtttcagc
      121 gcacattatc cacgggttgt tgtggcagaa tatgccagcg ttttccttat atcccggcgg
      181 atacgagctg atctcgccaa gctcgacatg gtaccttgta tatgcagggt tcaccagtac
      241 cataccgtgc tccgtatcaa gatatttcca tgccgaatcc agcgctttct gtgcttcccc
      301 cgtctcgact cctactccgg ccatgacaca gaatccctgt gtttcaatga atattttgcc
      361 ttcctcgttt tccctgcttc cgactttttc gccgaagtcg ttatatgcgc gcaggaacca
      421 ttctccgtca taaccgtact tcaggatgtt ctccttcatc ttccggatgt gttccttcgc
      481 agccttcgct tcttcgtatt cgcctatgtt ttcacagatc tttacgtact cgttgcctat
      541 atatacgaac atacccgcga tcaggacgga ttccgccacc ttgccgtctt tgctcgtggt
      601 ggtctggaac gattcgtccg gctccgtgga gaagcagttg aggttgaggc agtcgttcca
      661 gtctgcgcgt ccaatcagcg gcagtccatg cggtcccagg ttgttcacca catgatagaa
      721 ggatctgtgc agatggtcga acagggtagc cgccctgaaa ggatcgttgt cgtaaggcac
      781 catttcctca agtatgttga aatcgccggt ttcctttatg tatgccgcgg tggaaagtat
      841 cagccacagc gggtcgtcgt tgaagttgcc tcctatctcg ttattgccct tcttggtcag
      901 cggctggtac tggtggtatg ctccgccgtc ttcaagctgg gtggccgcga ggtcgaggat
      961 cctctccctt gccctttggg gtacctggtg gacgaatccc aggatgtcct ggttggagtc
     1021 cctgaagccc atgcccctgc cgattccgga ctcaaagtat gatgcgctcc tcgacatgtt
     1081 gaatgtgatc atgcactggt actggttcca gatgttcacc atccggtcca gcttttcatc
     1141 cccgctgtcg acgacgtacc tggacaggag accgtcccag tactgcttca gttcgccgag
     1201 cgcatcgtcc acctgccgcg gtgttgagaa tttctctatc atctttttgg ctttttcctt
     1261 gtttattata ttgttgcctt cccacttgtc ttcggggtca ttctcgacat agccgagcac
     1321 gaacactatt tccttctgtg cgcccggctg cagcgtgata tcgaaacagt gggacgctat
     1381 gggcgaccag ccgtccgcca tggaattgcg ggatttgcct tccacgacaa cctgcggagc
     1441 gtcaaaaccg ttgtactggc caatgaattc atacctgtcc gtatcaaagc cgtccacggc
     1501 cgtatttgcc gaatagaatg cgtaatggtt gcgcctttcc ctgtattctg tcttgtggta
     1561 gatcgtgttg ctttcgtatt ctacttctcc ggtgctgaag ttcctctgga agtttgtcat
     1621 gtcgtcatat gcattccaca ggcaccactc gatgaatgag aaaagtttta tttttcttgc
     1681 ctggctgcct ttgttcgata tggtgacctt gtggacttca ccgttgaaat cctgtggaac
     1741 gaaaatcagc acttcgccgc cgatgccgcc tctctccccg tatatgcggg tatatccgag
     1801 cccatgcctg cactcgtatt tatcaagttt cttccttacg ggagcccagg tcggcgacca
     1861 gaattcgccg ttttcctcgc ggatatagaa atatctgccg ccgctgtcca cgggcacgtt
     1921 attgtacctg tacctggtta tgcgcctcag tcttgcgtcc ttatagaagc aataacctcc
     1981 cgctgtattg gatataagtg agaagaattc ctggttaccc gaatagttta tccaggggct
     2041 tggtgtttcg ggcgtagtga taacatactc cctgtttgca tcatcaaaat aaccgtatct
     2101 cataagcacc ctccgctaaa gatctatttg cggccgctca cggccttttg caataactgg
     2161 aacttgatcg gtccaaccat tggaaaaggc cgccaacatg cactgtaata ttatgctccg
     2221 ttttacgttt caaatcaaca ggggtgggga aaccccaccc ctgctgacaa taggcatgtt
     2281 caggcgctta ttctttgccg actgccttgc gccattcgtc gtactgcctc tgctcctcag
     2341 caatgagctt gtcgacgccg gaagccttga gttcctgttc gaacttggca actgtcgaat
     2401 cgacatcaac agcgccgcac atgagctgtc ttgagtatgt gccgacaact gtcttacaag
     2461 cagaggactc tgtgcttacg ctctcgtcgc tgaatacgaa accgaggctg ttgagtacga
     2521 gaccttcgtc gttgaatttc ttgaacttct cccacttgtc aaggtcttcg ttgtcaagga
     2581 gcagatcctt gaactggtca ccatatctcc agccggggcc gaggttgtac ctgtcgacgc
     2641 ctgcctgggt ctgggtgatc ctgccgtcag cattgacgtt gtagtgggtg ccctcgatac
     2701 caaagttgat catgtttctc agcttggcat cggtatagag tttttcgatg aagaggaatg
     2761 ccctctccgg attcttggaa gctgcaggga ttgcaagcag agcacctgtg gtctcacggt
     2821 tggacataac cggcctggtg atgtcgacct gtacccactc gatgccggtg ctgccggtca
     2881 tctcagcatc ttttccgggc ttcagcgaag actcggctgc gaagaactta ccggtcttca
     2941 tctgctcagc ggtattttcc atggtagctg catcaggatg gatccagcct ttctggaagt
     3001 attcgcggag cttcttgtac atatcgatgg actcgggagc caggaagtgg ttgactatcg
     3061 tattgttcct gttgtccgga tagagagctc ccggtacgtt atcgtcactg accctgtccc
     3121 aatcgaggaa ctggaacggt gagtcgccgc cgactacgag cagcggagtg atatcgggct
     3181 cattggcctt tatcgtttca aagaagggct caacgtcttc catgctcttt acagaggaaa
     3241 gatcgaagtt gtacttgtca acgagttctt tcatgaacag gtagccccag ttatggacct
     3301 tctccttgtt ggtaggaaca gcataatggg taccattgat agcagaaccg ttgaggaagt
     3361 cctgaccgca gatctctacg attgcaggat acttcttgag gtagtcgttc agcggcagga
     3421 aatagccggc tgctgcgttc ttgtagtagt tggctgccca gtttgctgtg aagcagatat
     3481 cgaagggctc gcctgaagcc agcatggtgt tgaccttctg atcatacggg tcgccccatc
     3541 cgaatatctg gagattcagc ttgatgttga gcgagtcagc cagatacttg ttgacttcct
     3601 gcatgacaag gtcagtatcc gtttcctgct tgtcgccgac cagtgtccac gtgagttcaa
     3661 cagtgtcctt gaactgcggt gttgctgctg catcgccgct gctgtcggaa cctgagctgg
     3721 tgcttgtgcc ggccccggtg tctgtgcttg tgccgcttcc gctgccggta tcagtcgtct
     3781 gcttgtcgcc acagcctgtc agtatgagcg aaagtgcgac taccagcacg agagcgaggc
     3841 acaaatacct tttcatactg cgcatagaaa attacctcct aataatttta tttttatgtg
     3901 atgcaccgtt tcccgcggcc atgcgcggag aacaatgcca tcatccctta actgctccga
     3961 tagtaaggcc tttgacgaaa tatttctgga agaacggata tgccagtact atcggtccta
     4021 tcgaaatgac cgctatcgcc atcctgaggc tttcgctcgg caggttggca agtatctccg
     4081 tcatattgga gatctggttg ctgttgttca aaaggttctg caccgctatc agcgtctggt
     4141 acatgagata ctgtatgttg aaattttttg cgttattgac gaataccatc ggcagccaga
     4201 agtcgttcca gtagcccagg gtgcagaaca gtccgactgt tgcgagtccc gctttgcaga
     4261 gcggcaatac tatggtgaaa taggtcctga actctccggc gccgtctatc cttgccgatt
     4321 ctatgatcga ctcgggaacg gtcgtcttgt agaacgtcct cattatcatc atgtaccatg
     4381 tgttcatcag gtagggcacg atcattatcc atatcgagtc ctttatcggt acaagctgtg
     4441 tatatacgat ataccacggc acaagtccgc cgccgaatat catcgtgaag aatgcgaaga
     4501 acgaaaactg gtatctgtat ttgaacgaac tccttgacag cggatacgca tacagcgtta
     4561 tgactgcaag gctcagtatc gtgcccacga tggtaacgaa tatcgatacg ccgtacgccc
     4621 gccaaatggc gtcgccggcc ctgagcaggt aatcatacgc ttcagtgctg aactcgcttg
     4681 gtatgaactg atatccgtac tgcctgatgg actgttcgct ggtaacggat attgcgataa
     4741 cgagcagcac aggcgctatg catgtcaccg atacgattat gaaaaacagg tttatcagca
     4801 gattggtcga agtctttatt tcatttaccc tatatttctt cataaactta ctcattttac
     4861 cctcctcaga acagagcact ttccttgtcg attttcctaa ctatcaggtt ggctgttaat
     4921 accgttaaac agccaacgac tgcctggtat gtgcccgcag ccgaagacat gcctatgtcg
     4981 ttcgtgtttc tcagcgcatt gtagacatag gtatcaatga cctgggtagc cggctggagt
     5041 gtgccgctgt tcctgggtac attgtaaaag agaccgaaat ctgcattgaa tatcctgcct
     5101 actgcaagga gcgtgagtat tatcatcagc gtctgcagcg acggtatcgt tatatgtttt
     5161 atctgctgcc acttgcttgc tccgtcgatg gccgccgctt cgtaatactc gggatcgatt
     5221 cccgtgatgg ctgcaatata gaccactata ttgtagccgg tgtatttcca cagctggaag
     5281 aagactagta tgaacggcca gtatttcggc tcgctgtacc attccaccgg agcaatcccc
     5341 aaaggtgcaa gtatgctttt gtttataaag ccgttttcaa tgctcagcat cgaatatgca
     5401 agatagctga ctactatcca tgaaaggaag tacggcagaa aagccacgct ttggtatatt
     5461 ttcgacagtc ttttgttcct tatttccgtc agcgcgatcg cgcatgcgac cgggatgatc
     5521 agtcccagga ttatgaaagt aaaattgtac aggagcgtat tgcgcgtcat ttgccacgca
     5581 tagggagtct tcatgaagaa ttcgaaattc ttgaatccta cccactcgct tttcaccagg
     5641 cttttccaga atgtcccatg gaagcggtac ctcttgaatg cgataatgac gccgaacatt
     5701 gggatatagt tgttgatgat gagtattacc actccgggaa ggagcatcag gaaaagcggc
     5761 cactggtgcc tgagttggta aaaaaatcct ttctttttgc tggttttgat cgttttggcg
     5821 ctcgaaacag caagatccat cagaaaccac accttttagc aaagatttga tcttgattct
     5881 ggcaaagaat aagttttgta cacatgccta cggtataaat gttacagaaa ttttacagat
     5941 gaatgaatag aattggttgc aaaattgtgc acatatttgt tctcaaaggg tatgcaaaat
     6001 attacaacaa aatattacac agaagtcgga ttttattgct cgcggtattc cgtcggggtc
     6061 atgccnnnnn nnnnnatttt ttaaagagcc tggaaaaata gtgcgcgtcc gggataccca
     6121 ctttctcagc cacttcgtag gttttatacc ggacatcctt gagaagttcc tttgcttttt
     6181 ctatccgcag ttcgttcaaa taatccacaa aatttttacc catttctttt ttgaacatcc
     6241 tgctgatgta atatgtgctg acgaatgtat gctcggcgac ctcattgagc gttatcggct
     6301 cgtggtagtg tgtctgcagg tattcgaccg ctttcctcaa tatgagtttt atactcttgt
     6361 tgttgtaatt gttgaccttg gatactacgc tccttgcgga ttcttcgaga agcgtgtgca
     6421 gttcgtccgc attgccgcat ttttctataa gcccgtaaag gccgctcagt tctgtgtatt
     6481 ccaccttcct gccgtcctcg ccggacatga gcgataacct tatattgttg atcgatgtga
     6541 tggtgttcca gtagaaattc ttgatgaact ctttttgtga cggatcgatg ttccttatat
     6601 aactgtatat atctttcagc ctgccttcga ccgccgtttc gtttcctgat ttgatgcctt
     6661 cgagcagggc tttctccagc ttctccagca tggaatggtc atcatacctg aaaaacgtgt
     6721 ttacgtcgct gtggtatatt atcgagttgt tgccaagata gaacttgtgc tcgagcgctg
     6781 ccctgcattc gccgaatttc tgcgggagct cagtgaaatc gctgccttcg gaacttatgg
     6841 ctatcgtcac cgtgaaaccg aaacagttgt tgattatttc ctggagatac gtacactttt
     6901 tgttgatcgt ctcagccagt tcatcttcaa cgccttccct cggcatgaca ataaaagcta
     6961 tgccgacagt gtcgagcgtg acgtgcagca catcgaactt gtccgagaat atttcgatga
     7021 aggtgtttat tatcccgaac tggtacaaat gcctgtcgta ctggctgata tcgctgccgt
     7081 cgctttcttc ggcgtcgttc tggaccagga gcatgcggaa gtccccctgc ctcgtctgga
     7141 acaattccaa cttttccttt atatcatcac ggttcgtgtt gatctcataa atgatatcat
     7201 aaagaagctt ttcacgcagg acggaaatat tctgggtaaa cagctggctt agcttgtcga
     7261 actcttcgga tctctggttc tggaatttga gttccttaac ggcgcgcgat atggcagaag
     7321 tcagttcttc tatcctggac ggcttgagaa ggaagtctgt gactcccagc ttaagcgctt
     7381 catggacata atcgaaatcc ctgtggcctg taagtatgat gatcttgctg tcaggcacat
     7441 cctctttcat ctgctttatc atcgaaaggc cgtctgtctc aggcattttg atatcggtga
     7501 ttatgatgtc gggcttgtgc ttcctgatga gttccaggcc gtcaaggccg tcgccggctt
     7561 ccgctacaac ctcgcatccg aagttcttcc agttgataat gtttctcagg cctttgcgta
     7621 tgatcggttc atcatcgact atcagaacct tgtacataga agccctccct attatccagg
     7681 ttcactttgc agggtatggt aatcgtaact tttgtgaact ccccgaccct gctctctatc
     7741 ttcagaccgt actcttcccc gtaaaacagc tttatccttc tgttgacgtt ttctatgcct
     7801 atacttgtcc gctttttgcc gccaaggttt ttgaagtacg tatcattgtc cattgcgagc
     7861 cttgcattca gttcctccag ttcttcaggc tccatcccgg cgccgttgtc tatgacctct
     7921 acgcatacgc atcctttccc gtccttatac gcgttcagcc tgatggtgcc tctccccctg
     7981 ttcctttcga tcccgtggta taccgcattt tctatgagcg gctggatgag cagcctgggg
     8041 atttggatgc cgtaaacatc aggtcgtatg tccttaatta attctatttt atcgccgaat
     8101 ctccttcttt gcagtgaaat atacttgtct gcgtatttga attcctcttc gatcgtgatc
     8161 aggcggtcgt cccttccgat gctggcttcc atcaggtcgg acaggtccga tacggtattg
     8221 ctgatttcag ggacattgtt cagctgagcc atccagttga tagcctccag cgtattgaac
     8281 aggaagtgcg gatttatctg cgactgcaaa gctttcagtt cggcttcctt tcttgtaagc
     8341 tgctccctgt agacccagtt cacaagatgg tggatctcgc cggacatctc gttgaatgcc
     8401 ttctgcagga agccgatctc atcttccctg tccacttcta tacgtacgtt gccctcgccg
     8461 gactgcacct ttttcatgcc gttgaccagc ttgtttatcg gctttactat gtttaccgcc
     8521 gttataagcg ttacgactga aagaactgcc actgcagcga tgcaaagcat gatgatgttg
     8581 tttttcagag tattggcatc ctcgtacagc acatccagcg acacatacga tactatcttc
     8641 caccccgtga gttcctgggt tatatatgat atgaacatgc ctgccttctc gtcgactttc
     8701 tcgcccatgt agccgtctat gctttcgttc agcacgcctg acatataaga aagatcgtca
     8761 tagtcccggc tggctatcag ttccatgtcg ggtgaaagga taatgaagtt ttcgaaattg
     8821 ccggtcaggc cctggtatac ctcgtccagt atttccttct ttacatgcat gatcaggagc
     8881 cccagttcga cgaaatcgtc cctgtcattg atctgcctga tcatgtagat gtcctgtacc
     8941 ctgctgccgt ccgacacgat atacaggctg ggtttgccct ttttgcccct tgcctgttcg
     9001 agcatttcag caaacggaag atcgtcatgg gtgccggctt ccctggtatt gtcatcctgg
     9061 tagaagatcc ttccgtcatt ggcgtatatg cagatcgacc gtatttcggg cctggaaatg
     9121 actaccattt tgagatggct gttgactgag ttctggattt cgtaaagctt gaggggatct
     9181 tcaatgttgg tgccgctttt aagcacttca tatatttttt cttcataaag caggtcctgc
     9241 gatatctgta ccaggttatc catataatcg tcgagacgga gccggatcat gtgaaggata
     9301 tctctcgaat agtcggtcga gttttctttt atgatgcctt cagagttttt ataactcaga
     9361 aaaccaatca ggaccagcgg tatcagcatc tgtgttgtca gcagcagtac cagcttggtc
     9421 ttgatcgata tttttctgaa gatcctgaat aaacccctgt gagatttttt catgtgttga
     9481 cccccgaaag tcagattcac accgtccggc cttcccgtac aagccacgca tacctcgtca
     9541 gtaatccagg atgatgccgt tctctatggc agcctcccac atttcttccg cggtgatctt
     9601 gcccgagagg aaatacggga actgcgcggc tatatatttc tcccaggcag tcctgtccac
     9661 gtaggaatcc agcggaccga cgagcgtatc cgcttcctcc agcatgcgca tcccttttat
     9721 ggtcatgtct atataatcta tgtcatagcc ttccaggctc acagtgctga tcattcctgt
     9781 cctggatgcg aactgcgcgg cggattcggg tgacgtcagg aagcgcagca gcctgacgga
     9841 ggcttccctc tttttttcat catcccacgc ggcctggctc atgtggaagg tacctcctcc
     9901 aaggccgtat accatagtgt ttttattgct ttcataacct tttacgtatg ggaacggtat
     9961 tatgtcgaca gtatcgtcat caggctcgaa atccccgata taccatgagc cctgtactat
    10021 catcgcagcc ttcttttctt tgaaaagcac gttcctctca ttgttggtca tggtgaagca
    10081 ttccttcggg aatgctccga tatcgtacag ctcttttatg agattcagcg cgactatatg
    10141 gctttgcttt atcttcctgc cttctgtgaa cggaacttcc gtgccgtcct tcccgccgac
    10201 cacgggtaca aggttctggt aaaaatatgt gccttcggct tccgtattga atgcgatggg
    10261 tatgatgtta ttcctgttga atataaatac ggcttccttc agttcatcga agttctcggg
    10321 tattttgaca ttgtattttt taaagaggtc cctgtttacg aacatgcact cgaatattat
    10381 ttcaaaaggc agtccataga tcctctcatt atatgtggta tagctccaca tatccggatt
    10441 gaactgcctc ttccattccg ggtcgtccgt cagcaaaccg gtaagatcag ccacctttcc
    10501 gaccgtcacg agttttttta tgtctgagcc gggccacaag ccaaatacat cagggtcgtt
    10561 tcctgatgcg aagtctgttt tgatcttggg gaggaaatcc tccccaaaca gtgactcgtt
    10621 gacgacagtg atgtcggggt tcctgttttc gaactggctg aggacaagct tcagcgtttc
    10681 agctttgctg tcaacgccgc cccagctgca gatgaacctc agcgtgatat cggagtcttc
    10741 tgaaggtact attttccagt cagaatctga gaggtccgga gttgttttcg tacaggcgcc
    10801 ggacattgct gccatggaca tcaccagcag caatgccgtc agcgatcgca gtatcttcat
    10861 taccacagct cctttttgca acagctcccg tttagtctgc aatcgacgca tttgaccggt
    10921 ccatttgact ggttgcccgt ccaggtcatt tgaccaggcg gatcccgctt tcatccgcat
    10981 cgaccgtgat tctgtctccg ggttttatat ttccgaggaa gaactgttct gtaagctcat
    11041 cctcgatata tctctgtatg gtcctcctaa gcggccttgc cccgtatttg ggatcgtatc
    11101 ccttttcgat tatgaactcg cgggctttgt cggtgtagct tatgctgatc cccttctgtt
    11161 cagcttcggc agtcacttca tcgagcatca ggtttatgat ccgcatcagt tcgtccctgt
    11221 tgagttctgt gaacacgatg atctcatcga gcctgttcag gaactccggc ctgaatgtct
    11281 cttttataac gtccatgacc ttgctcctca ggtcgctgta gccatccctg ttgaacccga
    11341 tcccgttgga cttgagcgaa gtcccggcgt tcgatgtcat gattatgaca gtattttcga
    11401 agttcacgac acggccgtgg ctgtccgtaa ggcggccgtc ttcaagtatc tgcagcagta
    11461 tgttgaagac gtctggatgc gccttttcga tctcatccag cagtatgacg gaaaaaggct
    11521 ttctcctgac cttttcggta agctgcccgg catcgtcata gcccacgtag cccggaggcg
    11581 acccgatcag cttcgatacc gagtgtttct ccatatattc cgacatgtcg agcctgatga
    11641 gcgcgtcttc gctttcgaac agttcagctg caagcgctct gacgagctcg gtcttgccta
    11701 cgccggtcgg cccgacgaat atgaatgaag caggtttctt cttcttcctg aatccggccc
    11761 tgtgcctcct gatcgccttc gcgacgctgc tgacagcttc atgctggccg atgaccctct
    11821 tatggagcct ctcctcgagg tttatcagct tctcggcttc aacttccgtc agcctctgaa
    11881 cagggatctt ggtccatgat tcaatgacat gggccacgtc atccactgtc agcacagttt
    11941 ccttgttttt ctgctcaagc tcctgtattt gctggagcag cttgcattca cgcactttaa
    12001 ggtccgcggc cttttggtaa tcctctatgg agtctgccga gacagcgctt tctttgagtt
    12061 cctgcacctt tttcagttcg ctcttgagtt cctcaagttc gacaagcgta acgttcttta
    12121 gattcgccct ggagccggct tcgtctatta cgtctatggc tttgtcgggc aggaacctgt
    12181 cggttatata cctttccgac attctgaccg cagcctcgat gacatcatcg ggtatcctga
    12241 ccttgtggaa ttcctcgtaa taaccgcgga tacccttcag tatctctatc gcttcaccga
    12301 cagaaggttc atccaccagc acgggctgga accttctctc gagcgccgag tccttttcta
    12361 tgtgcttgcg gtactcattg agggtggtcg ctcctattat ctggatctct cccctggaaa
    12421 gggcgggttt gagtatgttg gcggcgttca tcg
//
LOCUS       DAOSCC010000047        12427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3442_length_12427_cov_4.802376, whole genome shotgun sequence.
ACCESSION   DAOSCC010000047 DAOSCC010000000
VERSION     DAOSCC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_12427_cov_4.802376"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLG72_03985"
     CDS             complement(<1..387)
                     /locus_tag="PLG72_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79991.1"
                     /translation="MEDTAALSVIVVEDSQYFADLTVRILKRSGLKLKSKVVSTRSAL
                     LEALKEDRYDIILSDNVMPDFSALSALEIKNNICSDVPFIIVSEDVSQKDLEEVFRNG
                     CDSYLPKDRIAELPEIVRKVLSASGRR"
     gene            complement(531..1631)
                     /locus_tag="PLG72_03990"
     CDS             complement(531..1631)
                     /locus_tag="PLG72_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ79992.1"
                     /translation="MYKVLLVDDMEISRRQVRRLNLWNGETNFCIAAEAQNGQEALNM
                     LRQDKFDLLITDIKMPKINGIELMKETAENGLCPCVVLLSDYEDFEYARLGIQYGAFD
                     YLGKPPEYNSMLDLLKRVEQYLRRKEQEEKLVRSLSEKLEEKIGDTFPSAELEQLIEA
                     VKTKQQDITGDIDRLCNAIEALYGNDPIKTGTALKKSMSELVSHLKTHYAWIVNFIDG
                     NKYRNLDFVTAGGSRTAREAFGDAVKELYESIRSFEYGNSEDQLENRICRYVLENVDT
                     EVSIEGISKVLFMNRKYISEVFRQRTGELLIDYITRIKMARAARLLCQSTRKTYEIAL
                     QLGYRDTEYFSRLFKKHMGITPSEYRNSSTES"
     gene            complement(1898..3055)
                     /locus_tag="PLG72_03995"
     CDS             complement(1898..3055)
                     /locus_tag="PLG72_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ79993.1"
                     /translation="MQYRSFGKLDFKVSALGFGAMRLPTRQMQGPGNRLDNNIDEPEA
                     IRMIRHAIDMGVNYVDTAYGYHGGQSEILVGKALKDGYRDRTVLATKSPVWLINGPSD
                     FDRLLDEQLGKLQTDHIDFYLLHSLNGNAWENTVLKHDLLSRMEAAKKAGKIRHMGFS
                     YHGDYDSFAKIIDGFDKWEFCQIQYNYMDIENQAGMKGLKYAASKGLAVVIMEPLLGG
                     RLANPPKDVKAVMEEADPAKSPVGWALDWLWSQPEVSVVLSGMSTMKQLEENIALADK
                     SEAGKFGRNELDVIDRIRRKYGERIAIPCTGCSYCVPCPNNVAIPRNFSIYNDGVMHD
                     DMEGARVAYNNFFNKENHASNCVRCGACETKCPQGIAIMDWLEKVDKALSR"
     gene            complement(3266..3342)
                     /locus_tag="PLG72_04000"
     tRNA            complement(3266..3342)
                     /locus_tag="PLG72_04000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3306..3308),aa:Arg,seq:acg)
     gene            complement(3461..3781)
                     /locus_tag="PLG72_04005"
     CDS             complement(3461..3781)
                     /locus_tag="PLG72_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOJ79994.1"
                     /translation="MKDKGLITLKYLQEYIRSKDFYPDRKKDYFMKLSEEVCELARAI
                     RKDLRPVSGGQIKETIEEEIWDVIYYALALANCCDIDLEKWIPVKEKINNEKFNTGIG
                     FDQM"
     gene            4116..5096
                     /locus_tag="PLG72_04010"
     CDS             4116..5096
                     /locus_tag="PLG72_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOJ79995.1"
                     /translation="MVFDENFSILYSDTLVPDVFISEYMPSLESDFIKVYIYCLFLGK
                     YRKSMTSEAIAKKLELRTERVKDALTRLEDLGLITRSADGNEITVNDIKEKEIKKVYR
                     AKTTSTPEEAELSIERSKKRSKFISSINNAFFQGVMSPSWYTDIDAWFDKYGFEEDVM
                     YALFKHCYDHNGLSRQYITKVADNWHSKNIKNSFDLDKYSMEAQKIKDIRGMIMKKLR
                     RHGSLTEYEDAYIEKWVSEYNFNADIIEEALRRTTSAQSPSFEYINRILTNWYEKGYK
                     TKEEILAAESSAKTPKQPRSKARGNVIPQKGNFEQRKYDDDYFDKFYEDV"
     gene            5248..6249
                     /locus_tag="PLG72_04015"
     CDS             5248..6249
                     /locus_tag="PLG72_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ79996.1"
                     /translation="MIKNELDQKRRKAIDELERKKAELYHRFPRLEEIEDEINSAGFR
                     YNKAILMGTVPADSAAEELASKIEELGREREQILTSNGYPADYLTPVFECSKCNDTGI
                     ISSSDGKGDTLCICYRQLLLDHLYDNSNLGISGNDGFASFNTELYPDTADEERYGIKK
                     SPRRQIIGILENCRQFTEKFSDPETRNLLFCGPTGTGKTFMAGCIAMELMRAGHTVLY
                     FTAPALFNTIYEYRYNSSNNEEWDPSAYKNILETELLIIDDLGTESPSATRYAELLNI
                     LDTRMSNDMRRPCKTIIATNIDLHKLFEYYDERIVSRITGSFKIYRFAGDDIRNLRR"
     gene            complement(6365..7447)
                     /locus_tag="PLG72_04020"
     CDS             complement(6365..7447)
                     /locus_tag="PLG72_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOJ79997.1"
                     /translation="MIDSGEKATAALAGENVKKLRPGVDGACVLFSVVIILFVLLGYR
                     IQSREFYSGILITEFMLILLPALVFAAISGVRFKEALRLDKFRPVNIPIVIGIMIFAI
                     PVATLLNVLNLLAVDSIFGKTVMTSLPAAENGVQLLVNILVIAGSAGICEEVLFRGVL
                     QRSFERFGTAGSILLAALLFSLTHLDFQKILGTFALGALIGYIVYRTNSLYCGMLAHF
                     TNNATAVFASYISGKMLEAFQLSDRMPSGPADISALFDTFAALPVEQKMIIFMVYGFV
                     FLFVAIVLTLLVYSLTRANRVSPVTMPHGSGLSYGTPAQGKAKGLLWLLPGIILILLW
                     FYVQANGFLGIQNAVTEAFRLLIGAV"
     gene            complement(7647..9080)
                     /gene="ypeB"
                     /locus_tag="PLG72_04025"
     CDS             complement(7647..9080)
                     /gene="ypeB"
                     /locus_tag="PLG72_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOJ79998.1"
                     /translation="MSKNSSGTIKEVFALDMREELPDSKKRPSNIRILAVLTALFVAA
                     AAWGYYQYRRAADLRQELDSQYNRAFFEMVGYVHNVESLLAKSMVSTTNERTAITLQE
                     AWRQAGLAQTNLGQLPVSQPVLASTSKFLTQVSDLSLSLDNQNIRGKSLSKEQYDMLK
                     KLYNYAVSLNKSLQELQEDLHVGRLRWGELANKGTRLFSKTKPGEMMKKIEAVGENFK
                     DYPTLIYDGPFSDHMTSGKARGLTGDDIDAETAKKKVFEFFGKDKVQEVTDLGKNDHG
                     QIKTYDFQVKLKNAPEDQAVYISVTQKGGHINWMLNNRPVKEEKLDMDEAKAAGIKFL
                     KEKGYENMEDTYYLKNDNTATINYAYRQDDVTMYPDLIKLKIALDNGEVVGIEAKQYF
                     MNHTERDIPEPAVSMEEAREKVNGKMKIYSSGLAYIPTDYNKEIFCHEFKGKLDDRDF
                     IVYINALTGEEEDILMIVNTPNGVLTM"
     gene            9264..10487
                     /locus_tag="PLG72_04030"
     CDS             9264..10487
                     /locus_tag="PLG72_04030"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ79999.1"
                     /translation="MKILFLAANTGGGHVKAAEAIIEYMEENVPGCETELIDALKYAN
                     AGIDRLITGTYLQTIRKAPWIYRKLYYLSEKNRSAAALARAIGGLLSSGLAGLFTDRP
                     PEAVICTHSIPLQMVSKLKRAGIINKPVIAVVTDFASHSFWCLKETDAFIVAHEDVRF
                     EMAALGIDKNRIHAFGIPVSQQFTRGNGANGSRTYGHHRYNVFLMGGSLGLCSMQETF
                     SALLKIDRDIGITAVTGYNTKLKRRLEQIAAASGKDARVFGYTRNISELMDKADLIIT
                     KPGGVTIAEALVKKLPIFFMDAIPGHEERNARFLLESGAACWLPPGARMPEALEQILD
                     SPKMLQSMSESAAKLARPNACAEITRLVTDLIRKKNGFQISKVGIPGTVNIRSGQFAG
                     INLSEPKAVRNAHQG"
     gene            complement(10517..11554)
                     /locus_tag="PLG72_04035"
     CDS             complement(10517..11554)
                     /locus_tag="PLG72_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOJ80000.1"
                     /translation="MKLLYRRKGLGKAGRLLISALLISMLLGGCGERAMQTTVEDTGN
                     KAGDGSGQAIEYIYGIPIDDNAAVVTVSVADVYEKPDVKSPRITQVLCNQPVSVISEN
                     NGWTRVKTVSGVTGWIRSKFISDDISSISGRIYTYRIVVTSREKTVYSRPGSGATMVN
                     AYMGTEFYAFNSSDDAYEVFLPENRTGWIKGSGIIRIDPPYGEIPVTNAGDFASTALK
                     FKGISFMLNGLTSLGIDAPGLVYICARINGVDLPVTLEGQLNAGVEIPLDKVQAGDLV
                     FLAEKADVSETTDTSETDDSEEVEITSVGICTGGSNYIYAGRKTGYVAIGDLYRENPD
                     GIAVKARRIFN"
     gene            complement(11556..>12427)
                     /locus_tag="PLG72_04040"
     CDS             complement(11556..>12427)
                     /locus_tag="PLG72_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOJ80001.1"
                     /translation="AKRNLLSGASMMYGIKAGSVSDVKAALDDVRADLERLSRQLQSG
                     LEEAAAMDVPLPGIFSQRELDELIYDSDINWLEDGWKTESESLRRKILDTALREKYCE
                     GQLDEDKGETDRMQRVEEEIAAVREKIGKLENTGKALKLALEVLTEAAEEIRLSNAPE
                     LNNRLSVIISGLTGGRYTDLRGDNKLLLKASDPQQGDVRNVSVLSGGTADQMYLALRL
                     AVADMLTSGGESLPLVMDEVFSQFDDKRTELALKYLYRERRDRQILLFTCKMREVELA
                     REIYGNEMYFVEL"
BASE COUNT         3096 a         3274 c         2788 g         3269 t
ORIGIN      
        1 gcgacgccct gaagcgctga ggacctttcg tacgatttcc ggcagctctg ctatcctgtc
       61 tttggggaga taggaatcac agccgttccg gaatacctct tcaagatcct tctggcttac
      121 atcttcagaa acgatgatga acggtacgtc gctgcatatg ttattcttta tctccaacgc
      181 gcttaatgca ctgaaatcag gcataacatt atcgctgagg attatgtcat accggtcttc
      241 cttcaatgcc tcaagcagtg cagatcttgt cgatactacc ttgcttttga gtttcagtcc
      301 gcttcgcttc agtatcctga cggtcagatc cgcaaaatac tgggaatctt cgaccacgat
      361 cactgacagc gcagctgtat cttccataag catccaccat tcattcatga ttaggctgac
      421 tgacgccaaa taaaaaacag gacatcatta tttccctgtc tgtaatgata ccctgtcgga
      481 aagcaatgtt aaatgaatta tttcttgtat ttacctagaa atttgtcaga ttacgactct
      541 gtcgaggaat tcctatactc gctgggagta atccccatat gctttttgaa cagcctgctg
      601 aaatactcgg tatccctgta tcccaactga agggctatct cgtatgtttt cctggtgctc
      661 tgacatagaa gccgtgccgc cctggccatt ttaatccttg tgatgtaatc tataagcaac
      721 tcgcctgtcc tttgcctgaa tacttcgctg atgtatttcc tgttcatgaa aaggactttt
      781 gatatccctt ctatggaaac ctcggtatcg acattttcaa gcacataacg gcatatcctg
      841 ttttcgagct gatcttcgga gttcccatat tcaaagctcc tgatagattc atacaactcc
      901 tttaccgcat ctccgaaagc ctcccgcgct gtccgggatc cgcccgccgt tacaaaatca
      961 aggttcctgt atttattgcc gtcgatgaag tttacgatcc aggcataatg cgtctttaag
     1021 tgcgaaacca gttcagacat tgattttttc agtgcggtgc cggtttttat cggatcattc
     1081 ccgtaaagag cttcgatggc attgcagagc ctgtctatgt cgcccgttat atcctgctgc
     1141 ttcgttttaa ccgcctctat cagctgctcg agttccgccg acgggaaagt gtccccgatc
     1201 ttttcctcaa gcttttcgga aaggctcctt acgagttttt cctcctgctc cttcctacgc
     1261 aggtattgct cgactctttt cagcaggtcg agcatgctgt tgtattcagg aggtttgcca
     1321 aggtagtcaa aagccccgta ttgtatgccg agcctggcat attcaaaatc ctcatagtcg
     1381 ctcagaagca ctacgcaggg acaaaggccg ttttcagccg tctccttcat cagctcgatc
     1441 ccgttgatct ttggcatctt gatgtctgtt atgagaaggt cgaacttatc ctgcctcagc
     1501 atattcaggg cttcctgacc gttctgggct tcggcagcta tgcagaaatt cgtttcgccg
     1561 ttccagagat tgagccgtct tacttgtcga cggctgatct ccatatcatc aacaagtaat
     1621 accttataca attcttcacc ccatcaacac ggctgtgtct gtaacgttta cagccttttt
     1681 tctttggttt tcggactttc gttccgcatg atcagacatt ttgtcatatt tttgccattg
     1741 tattattatt ctaccatata tgtcgtcaaa ttcatataag gaggtaaagg atttaatttg
     1801 acgcgtatgg ttcggctgca tgcatcattc aataaaaaga cgtcaggcag tctgccgttc
     1861 ggcaggctgc ctgacgtctg tcttaagcga taactcttta tctgctcagt gctttgtcga
     1921 ctttctcaag ccagtccatt atcgctatcc cctgcgggca tttcgtctca catgctccgc
     1981 atctcacaca gttgctcgca tggttctcct tattgaagaa gttattatat gcgacccgcg
     2041 ctccctccat gtcatcatgc atcacgccgt cattgtatat cgagaaattc ctgggtatcg
     2101 cgacattgtt ggggcaaggc acgcaatagc tgcagcccgt gcatgggatt gcgatccttt
     2161 caccgtattt tctgcggatc ctgtcgataa catccagttc gttcctgccg aattttccgg
     2221 cttcagactt atctgccagc gctatattct cttcgagctg tttcatggtg ctcatcccac
     2281 tgaggaccac agatacttca ggctggctcc acagccagtc cagcgcccag ccgacaggag
     2341 atttggccgg gtccgcctcc tccattactg ccttgacatc tttgggcgga ttggccagcc
     2401 tgcctccaag caacggctcc attatcacca cagcaaggcc ctttgaagcc gcgtatttca
     2461 gccctttcat ccctgcctgg ttttcaatgt ccatgtagtt gtactggatc tggcagaact
     2521 cccatttgtc aaaaccgtcg attatttttg cgaatgaatc gtaatcgccg tggtaggaga
     2581 agcccatatg cctgatcttt cccgcctttt tagccgcttc catccggcta agcaaatcat
     2641 gcttcagtac ggtattctcc catgcattgc cgttgagcga atgcagaagg taaaagtcta
     2701 tatggtcagt ctgcagtttg cccaattgct cgtccagcag cctgtcgaaa tcggaaggcc
     2761 catttatcag ccagaccggt gatttcgtcg caagcaccgt cctgtccctg tatccgtcct
     2821 tgagcgcttt tcccacaagg atctcgctct gcccgccgtg gtaaccatag gccgtatcga
     2881 catagttgac tcccatatct atagcgtggc ggatcatcct gatcgcttcc ggttcatcta
     2941 tattattgtc gagtctgttg cccggtcctt gcatctgcct ggtcggaagt ctcattgccc
     3001 cgaatccgag tgccgacacc ttgaagtcca gttttccaaa tgatctgtat tgcatatgaa
     3061 ccctcctgtc tctggtaatg atcgactgaa atgtatttat gtatttacat ttagtcccgg
     3121 aagaaaaatt acgttattta tataatacta ttaatatcac tgaaatatca actttctcca
     3181 aaaaaattaa ccttcttact atgttagccg caaacaaaac cagagcctgt cagtatttat
     3241 actgacaggc tctgacccga tgatttggtg cgcccaggag agctcgaatc cccagccttc
     3301 tggtccgtag ccagacgctc tatccagttg agctatgggc gcgtatctcg cagcgatgcg
     3361 gtatgccaaa acagctttcc gtaactgtat atattataat aatactttct tgcgttaaaa
     3421 tcaactgtat ttatgtggtt cagcgctagt tgcaatttta tcacatctga tcgaatccga
     3481 ttccggtatt gaatttttca ttgtttattt tctcttttac aggtatccat ttttccaggt
     3541 ctatatcaca gcaattcgca agcgccagcg cataatagat cacatcccat atttcctctt
     3601 ctatcgtttc ctttatctga cctcccgata ccggccttaa atccttgcgg atagctctgg
     3661 caagctcgca gacttcttcc gacagtttca taaaataatc tttttttctg tcaggataaa
     3721 aatcctttga tcttatatac tcctgcagat atttcaaagt aataaggcct ttatctttca
     3781 agcaattacc tcctcccgat caactctatc ctccagttat tttaaacagg caatcgcagt
     3841 accagtcccc gatccctgcc gaagcgagga acggcagccg gcaggcatta ccttaaggcc
     3901 ggcaccaata cttttccttt agcatatagt aataaattcg atttgtctac cagaataata
     3961 aaaccatatg agttcatcag caatgcttgc cagcggatag tccgacgaat atgcagacaa
     4021 caaattcaca ttgttcaatg ggaacacctt gtatgctata ataagttaaa gataacacaa
     4081 taagaatggt atacaaatca gtgaggtcat ttttcatggt atttgacgag aacttctcaa
     4141 tactctattc cgatacgctg gtaccggatg ttttcatatc cgaatacatg ccgtcgcttg
     4201 aaagcgattt tattaaagta tacatatatt gcctctttct cggcaaatac aggaaaagca
     4261 tgacgtcgga ggcgatcgcc aaaaagctcg aactccggac ggagcgcgtg aaagacgccc
     4321 tgacgcggct tgaggatctc gggctcataa caaggagcgc cgacgggaat gaaataacgg
     4381 tcaacgatat aaaggaaaag gaaatcaaaa aagtctacag ggccaagacc acatccactc
     4441 ccgaggaagc ggaactgagc atcgaaagga gcaaaaagcg cagcaaattt atttcaagca
     4501 taaacaacgc atttttccag ggcgtgatgt ctccttcatg gtatacggat atcgacgcat
     4561 ggttcgacaa gtacggattc gaagaggatg tcatgtatgc ccttttcaag cactgctatg
     4621 accataacgg cctgtcgcgg cagtacataa caaaagttgc cgacaactgg cacagcaaaa
     4681 acataaagaa cagcttcgac cttgacaaat actcgatgga agcccagaag atcaaggaca
     4741 tacgcggcat gatcatgaaa aagctaaggc gacatggatc gcttacagaa tacgaagacg
     4801 cctatatcga gaaatgggta tcggaataca acttcaatgc tgatataatt gaagaagcct
     4861 taagaaggac gacatcggcc caatcgccaa gtttcgaata cataaacagg atcctgacca
     4921 actggtacga gaaaggctat aaaacaaaag aagagatact cgctgccgaa agctcggcca
     4981 aaaccccgaa gcagcccagg tccaaagcca gggggaacgt tattccgcag aagggcaatt
     5041 tcgaacagcg caaatatgat gacgactatt ttgacaaatt ttacgaggat gtgtaagccg
     5101 ggagatgcac atggcacggg agcaaaaccg ttcaccgcag atccatacgc gctgtttgca
     5161 gggcaaacga acacagcatt tgatacccgg catatataag aaccggaaag gatgataacc
     5221 atcagtatca gttacagcat acaccagatg ataaaaaacg agctcgatca aaaacgccgg
     5281 aaggcgatag atgagctgga aagaaagaaa gccgaactgt atcaccgttt cccccgtctt
     5341 gaggaaatag aggacgagat aaacagcgcc ggcttcaggt acaacaaggc catcctgatg
     5401 ggcacggtac ctgccgacag cgcggcggaa gagcttgcaa gtaaaataga agagttgggc
     5461 agggaacgcg aacagatact tacatccaac ggatatccag ccgattacct gacacccgtt
     5521 ttcgaatgca gcaaatgcaa cgatacagga atcatcagtt catccgacgg gaaaggagat
     5581 acactttgca tatgctacag gcagcttctc cttgaccacc tgtacgacaa ctcgaacctg
     5641 ggcatcagcg gcaacgacgg atttgcaagc ttcaatacgg aactgtaccc ggacacagcc
     5701 gatgaagaaa ggtatggcat caaaaagtct ccccgcaggc agataatcgg catactggaa
     5761 aactgcaggc agttcacgga aaaattcagc gatcccgaaa ccaggaacct tcttttctgc
     5821 ggcccgaccg gcacaggaaa gaccttcatg gccgggtgca ttgccatgga actgatgaga
     5881 gcggggcata cggtactgta cttcacggcc ccggcattat tcaacactat atatgaatac
     5941 aggtataatt ccagcaacaa cgaagaatgg gacccgagcg catataaaaa catcctcgag
     6001 accgaacttc tgatcataga cgacctgggg acagagtccc cctcggccac gcgttatgca
     6061 gaactgctga acatactcga cacaagaatg tccaacgaca tgcgcaggcc ctgcaaaacg
     6121 atcatagcca cgaacatcga cctgcacaag ctcttcgaat actatgacga aaggatagta
     6181 tccaggataa caggcagttt taaaatttac cgctttgcag gcgacgatat ccggaatctc
     6241 agaagataag gccatatcat gacaacctgt gagcggaaac cgtcgctgtc aggatacatc
     6301 gtcataatat aaaaccttgc cttgcaagac aacttttttt gccggatcgg tcaatatacc
     6361 ggcatcacac ggcgccgatc agcaacctga acgcttcggt gacagcgttt tgtatgccga
     6421 ggaatccgtt tgcctggaca taaaaccaga gcaggatcag gataatcccg ggcagcagcc
     6481 ataaaagccc ttttgccttc ccttgcgcag gcgtaccgta tgaaagtccg gaaccatgcg
     6541 gcattgttac cggagaaaca cgatttgccc tcgtcagcga atagaccaga agtgtaagaa
     6601 cgatcgcgac aaaaagaaat acaaaaccat agaccatgaa tatgatcatt ttctgctcca
     6661 ccggcagcgc ggcaaacgtg tcgaacaacg cactgatgtc tgccgggccg gatggcatcc
     6721 tgtctgacaa ttgaaaggcc tccagcattt tccccgatat gtatgaagcg aaaactgctg
     6781 ttgcattgtt tgtaaaatgt gcaagcatac cgcagtacag tgagttggtc ctgtacacaa
     6841 tatatccgat cagtgcacca agcgcgaatg tcccgagtat cttctggaag tccaggtggg
     6901 taaggctgaa taaaagggct gcgagaagta tcgatccggc cgtcccgaaa cgttcgaacg
     6961 accgctgtaa tacgcccctg aacaggacct cctcgcagat cccggcactg ccggcaataa
     7021 caagtatgtt cacaagcaac tggactccgt tctcagccgc cgggagcgac gtcataaccg
     7081 ttttgccgaa tatcgaatcc actgcaagca ggttcagcac atttagcaac gttgccacag
     7141 gaatggcaaa aatcatgatc ccgataacga tgggaatgtt tacgggcctg aatttgtcaa
     7201 gtctgagcgc ttctttgaac ctgacccccg atatcgccgc gaacacaagc gcaggcagca
     7261 gtataagcat aaattcagtg ataagtatgc cggaataaaa ctcccttgat tggatcctgt
     7321 agcccaggag tacaaataat atgataacca ctgaaaataa aacgcatgcc ccgtcgacac
     7381 ccggcctcag tttttttaca ttctctccgg caagggcggc agtcgctttt tcgccgctgt
     7441 cgatcatggc gcacctccga aaataaagcc gctctcatgt ctaagtacgg ctcatctcta
     7501 agattaactc tctatatatt accgttttat taaagtaaat gcaatcggtt ccatttcttt
     7561 ttcttcctgg cgtgctgctt tttcggtgcg ctgatgtttt gcagtgcaat gtcagatacc
     7621 gggctattgc caaaaccggg tagttattac atcgtaagca ctccgttggg agtattgacg
     7681 atcatgagga tatcctcttc ctctccggta agggcattga tatatacgat aaaatccctg
     7741 tcgtcaagct tccccttgaa ctcatggcaa aagatctcct tattgtaatc cgtaggaatg
     7801 tatgccaggc ctgagctata tatcttcatt ttgccgttca ccttttccct ggcttcttcc
     7861 atgctgaccg ccggctcggg gatatcgcgc tccgtatggt tcataaaata ctgctttgcc
     7921 tcaatgccta cgacttcccc gttgtcgagg gctatcttca gtttgatgag gtccgggtac
     7981 attgtgacat cgtcctgcct gtaagcgtaa tttatggttg cggtattgtc gttcttgagg
     8041 tagtaggtgt cctccatgtt ttcgtatccc ttctccttca ggaatttgat cccggctgcc
     8101 tttgcctcgt ccatgtccag tttttcctct ttaacaggcc tgttgttgag catccagttt
     8161 atatgcccgc ctttctgtgt cacgctgata taaactgcct ggtcctcagg ggcattttta
     8221 agtttgacct ggaaatcgta agttttgatc tgtccatggt catttttgcc caggtcggtc
     8281 acttcctgga ctttgtcctt tccgaagaat tcgaatacct ttttctttgc tgtttcggca
     8341 tctatatcat cacctgtcag tccgcgggct ttgccggacg tcatatggtc ggagaacggt
     8401 ccgtcatata tcagcgtcgg gtaatccttg aagttctctc cgacagcttc gatctttttc
     8461 atcatttcac cgggtttggt cttgctgaac agcctcgtcc ccttgttcgc aagttcgccc
     8521 cacctcagcc tgccgacatg cagatcttcc tgcagttcct ggaggctttt gttgagggat
     8581 acggcatagt tatagagttt tttcagcata tcatattgtt ctttgctcag cgacttgccc
     8641 ctgatgttct ggttatccag cgaaagacta agatcgctga cctgggtcag gaacttcgac
     8701 gtgctcgcca gcaccggctg cgagaccggc agctgtccaa ggttcgtctg tgccagtccg
     8761 gcctgtctcc aggcttcctg caaagtgatg gccgtccttt cattggtcgt gctgaccatg
     8821 gattttgcga ggagcgactc gacattgtgc acatagccca ccatttcaaa aaaagccctg
     8881 ttgtattggc tgtcaagttc ctgcctcagg tccgccgccc gcctgtactg ataataaccc
     8941 caggctgctg cggcgacgaa cagggcggtg agtaccgcta atatccttat atttgatggt
     9001 cttttttttg aatccggcag ttcttccctc atatccagtg caaatacctc cttgatcgtt
     9061 ccggaagagt ttttggacaa atatcctcca ttattccaaa catccgccgt tgttccctct
     9121 taataccgtg gacttgtatt gattattctc tgcacatcac gggaaattat tcgagccgga
     9181 gcacgagtac acgttggggc aggataaatc tgtaataatg cccgggactt ttgtcaaaaa
     9241 tgatatgcgg aggcagacag cagatgaaaa tactttttct ggcggcaaat acaggcggag
     9301 gccatgtgaa agccgcggag gccatcattg aatacatgga ggaaaatgtc cccggatgcg
     9361 agacggaact gatcgatgcg ctcaaatacg caaatgcagg tatcgaccgg ctaataacgg
     9421 gcacatacct ccaaactatc agaaaagcgc cctggatata caggaaactg tattaccttt
     9481 ctgaaaaaaa caggtccgct gcagctcttg caagggcaat aggcggcctt ctttcatccg
     9541 gcctggccgg tctcttcact gacaggccgc cggaggcggt catctgtaca cattcaatac
     9601 cgctccagat ggtctcgaaa ctgaaaagag caggcatcat aaacaaacct gtgatagccg
     9661 tggtgaccga tttcgccagc cacagcttct ggtgtctcaa agagacggac gctttcatcg
     9721 tcgcccatga agatgtcaga tttgagatgg ccgcactggg gatcgacaag aaccgcatac
     9781 atgcattcgg catacccgtt tcccaacagt tcaccagagg aaacggcgcc aacggatcca
     9841 gaacatacgg gcaccacagg tataacgttt ttcttatggg agggagcctg ggcctgtgca
     9901 gcatgcagga aacattttcc gcactgctga agatcgaccg ggatatcggt atcacagccg
     9961 ttacagggta taatacgaaa ctgaagcgtc ggctcgagca gatcgctgca gcttcaggaa
    10021 aagatgccag ggtattcggc tatacacgca atatcagcga gttgatggat aaagccgacc
    10081 tgatcattac aaagccgggc ggcgtcacga ttgcggaagc gctggtcaaa aagctgccta
    10141 tatttttcat ggatgccatc cccggacacg aagaaaggaa cgcaaggttc ctgctggaat
    10201 ccggcgcggc atgctggctg cccccgggcg ccagaatgcc cgaagcactt gagcaaatac
    10261 tggacagccc caaaatgctg cagagcatgt ccgaatcggc agcaaagctg gcaaggccga
    10321 atgcatgcgc agagataacc cggcttgtaa cggatctgat cagaaagaaa aatggatttc
    10381 agatcagcaa agtcgggatt ccaggaactg tgaatatacg atccgggcaa tttgccggca
    10441 taaatctgag tgagccgaaa gcggtacgga atgcccatca gggctgaaat cgatgaaacc
    10501 ggtaagtttc cggctgtcag ttgaaaatcc gccgtgcctt aactgcaata ccgtccggat
    10561 tctcgcggta caggtcgcca atcgcgacat atccggtttt cctgcccgca taaatgtaat
    10621 tgctgccacc ggtgcatatg cctacgctcg ttatttccac ttcctcgctg tcatccgttt
    10681 ccgaagtgtc tgtcgtttca gaaacgtcag ccttttctgc gaggaacacc aggtctcccg
    10741 cctggacttt atcaagcggt atttccacgc ctgcgttcaa ctgtccttca agggtcaccg
    10801 gcagatccac cccgtttatc cgggcgcaga tatagacaag tccgggcgca tcgatcccga
    10861 gcgaagtcag gccgttgagc ataaagctta tccctttgaa tttcagtgca gtcgaagcaa
    10921 agtcgccggc atttgtcacg ggtatctcgc catatggcgg atcgatccgt atgatgccgc
    10981 ttcccttgat ccatcctgtc ctgttttccg gcaggaagac ctcataggcg tcatcactgc
    11041 tgttgaacgc atagaattcc gtgcccatgt acgcattgac catggtggcg ccgctgccgg
    11101 gcctggagta aacagtcttc tccctgcttg tcacgacgat cctgtaagta tatatcctgc
    11161 ctgaaatact cgatatgtca tctgatatga atttcgatct tatccatcct gtgacgccgc
    11221 tgaccgtttt cactcttgtc cagccgttgt tttcgctgat aacgctgaca ggctggttgc
    11281 agagcacctg ggtgatgcgc ggcgacttga cgtcaggctt ctcgtacacg tctgcaacgc
    11341 ttacggtaac taccgccgcg ttatcatcta tcggtatccc atagatatat tcaatggcct
    11401 gcccgctccc atctccggct ttattgccgg tatcctccac agttgtctgc atggcccttt
    11461 cgccgcatcc gccaagcagc atggagatga gcaatgctga gatcagcagt ctgcccgctt
    11521 ttcctaatcc ttttcttctg taaagcaatt tcatatcaca actccacaaa atacatttcg
    11581 ttcccgtata tttcccgcgc cagttccact tcccgcattt tgcaggtaaa cagcagtatt
    11641 tgcctgtccc tgcgctctct atacaagtat tttaacgcaa gttcggtccg tttgtcatca
    11701 aactgcgaaa atacctcgtc catgaccaat ggcaggcttt cgccgccgga tgtaagcata
    11761 tcggctaccg caagcctcaa tgcaaggtac atctggtctg ccgtaccgcc gctgaggacc
    11821 gacacgtttc gcacatcccc ctgctgtggg tcggaggctt taagcagaag tttgttgtct
    11881 cccctgaggt cggtgtatct ccctccggtc agccctgata ttatgacgct caggcggttg
    11941 tttagttccg gcgcattgct gagccggatc tcctccgcgg cttcggtgag cacttccagg
    12001 gccagtttca gtgcctttcc cgtgttttcg agcttaccga tcttttccct caccgcggcg
    12061 atctcttctt ccacccgctg catcctgtcg gtttctccct tgtcttcatc aagctgtcct
    12121 tcacagtatt tctctctcag cgcagtatcg aggattttcc ggcgcaggct ttccgattcg
    12181 gttttccatc cgtcctccag ccagtttata tctgaatcat atatcagttc atcaagctca
    12241 cgctgtgaaa atatcccggg caaagggaca tccatggcgg cagcctcttc aagcccgctc
    12301 tgcagctgcc ggctcagcct ttcaaggtct gcccgcacat catccagggc tgctttgacg
    12361 tccgaaacgc taccggcttt tattccgtac atcatggatg ctcccgaaag cagattgcgc
    12421 tttgccg
//
LOCUS       DAOSCC010000048        12352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3461_length_12352_cov_4.936651, whole genome shotgun sequence.
ACCESSION   DAOSCC010000048 DAOSCC010000000
VERSION     DAOSCC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3461_length_12352_cov_4.936651"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLG72_04045"
     CDS             complement(<1..339)
                     /locus_tag="PLG72_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80002.1"
                     /translation="MELQTERLILRPWLESDAESLYEYAKDPRVGPMAGWPPHTSVEN
                     SLGIIRNVLAVDENYAVCLKEDNRAIGSIGLMVGKASNLDLPETEGEIGYWIGVPFWG
                     KGLIPEAARAL"
     gene            586..1407
                     /locus_tag="PLG72_04050"
     CDS             586..1407
                     /locus_tag="PLG72_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80003.1"
                     /translation="MDYKNHIKRAIDYIEENLKNEITIADCAKVSGYSNYHFIRVFKE
                     ATGLTPADYIRKRRLTEIVRNMRKGVPVCEIAFEYGFNSKENFTRAFFAEHHILPSEY
                     KAALNSLKLYEPIIFEAEPFEVKPELTYLEPFELVVYKSDEDCPPKFWNKYNSRKWSK
                     KLTGGKTCADFGVCVWNQNENKLDYFIGVRKSDALGDTGNTIELTVPGGLYAVFATPQ
                     SSHFDFINTIHRTWDYIYNEWLPNSGYEHTGGCEFETYIESSRTYSEKIYIPLKS"
     gene            1610..2539
                     /locus_tag="PLG72_04055"
     CDS             1610..2539
                     /locus_tag="PLG72_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80004.1"
                     /translation="MKKYLNISWDGVRDSTGYLFSFAKSLSAAVKNSPYSEKAEDIVA
                     TSGFAFRMWVSRDLCPSATSIWDFESQKPWVENGGLLCDYVGRYWEQDNIEEERRLKA
                     ISIIKASIDRGIPAVSWDIGVPEWGLITGYDDDSQMFSVLAVNDGNPDPANPALMPYD
                     LLGKRELPILSVLTVTGISGKTDEQILQDTKRLAAYHLKGGEWCENANGLEAYPALIR
                     IFDEDPGLASSWNAEYFLGTYGALKEYAYRYFEKAGETKLAELYRSVYDAWMEAFRIK
                     TNEDASIPETRARIASLLRSAYENEKEAVRIME"
     gene            complement(2570..3691)
                     /locus_tag="PLG72_04060"
     CDS             complement(2570..3691)
                     /locus_tag="PLG72_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ80005.1"
                     /translation="MNALFLTITAGQGHNSTSYALARYLETQGVDCTVLDTYKYLSKL
                     IGEIVDVGYASIAKHSPKLHLIMYDNAEKVSGNEKTRKTFLPYAIAEASKSKMLKYIK
                     LKKPDVIICTHVFTAIIISQMRKDGILDGRIPVFGIITDFTLHPFWEDTVLDYYVVAN
                     ELVVHEARRKGIDADKLLPFGIPVKEEFSVDIPASEARARLSIKDKLTMLVISSSVGF
                     GNIPEILSDMDKVPMDFQIVVICGRNTRLSQKIIGTPYSKDVVVEGYVDNMELYMDAA
                     DVIITKPGGLTVSEALTKRKPLIVTDPIPGVENRNAYFLMNNGLAVYAGRYARICDVI
                     MQMYSRPEKLEQMRQAQETYGKRHSAKNMGDFIIKLIDM"
     gene            complement(3839..5092)
                     /locus_tag="PLG72_04065"
     CDS             complement(3839..5092)
                     /locus_tag="PLG72_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ80006.1"
                     /translation="MSILARLTRVWLTRQLQPFTFKSEYDGKLDFADCKGLGLYVHIP
                     FCRQLCSFCPYCRQLYDEKACDEYIDHLIREIRLAGSQLKEKNRVTSLYFGGGTPALA
                     AERIGEIISVIGEYFDITEGTGLELHPDDVSEETLTVLRAAGVTKISIGIQSFGDKFL
                     SILGRKGLDPVRVEAALEKVPFETVSMDFIFALPGQTFDDLKADIDTAFSIGADHVAI
                     YPFIDFTFAGSGVSPMGKQEKRKLLDSITLYCLSKGYTRDSIWTFSNKPGAKYSSMTR
                     DNYLGFGCSAVTLLRDQFRINTFSVKEYQERIRNSMLPTSLTLRFTERQRMIYYLFWS
                     AYSTRVDPHKFEDFFGVPLEKVYGFELWLAKKLGFIVEEDGVYHMTLKGAFYYHHYES
                     YYTLAYINKMWGIMRHTAFPEKIVL"
     gene            complement(5151..5294)
                     /locus_tag="PLG72_04070"
     CDS             complement(5151..5294)
                     /locus_tag="PLG72_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80007.1"
                     /translation="MFYGLMWSQGTAVNLFRKLGYDPVDQCGSTADGGVKRRDYIFEK
                     ILG"
     gene            5958..6098
                     /locus_tag="PLG72_04075"
     CDS             5958..6098
                     /locus_tag="PLG72_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80008.1"
                     /translation="MQYPDAVRTALDYIEQNLLKGKPLPRSDKFGKDHTMYGEKYGRG
                     IR"
     gene            6095..6223
                     /locus_tag="PLG72_04080"
     CDS             6095..6223
                     /locus_tag="PLG72_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80009.1"
                     /translation="MICVAYFENIDMFRDLAKTNRRMLKYIIQNRERIENSLDSLR"
     gene            6333..6974
                     /locus_tag="PLG72_04085"
     CDS             6333..6974
                     /locus_tag="PLG72_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ80010.1"
                     /translation="MERNYGAEIDSLNAEISSLKKELQELGDVIRSYMHSRAKQAEAK
                     PMKRVCQMKDMHPDKRLSGKMEELCTLTDEKGVTGMITYLGVYCSGGRQSNWIRNEVC
                     TDDLLALIENSTAEKVLNCIGSSDRLNILLALLKKPMSVAEIVNECGLNSTGQAYHHM
                     KPLLAADLIIEDENNKGVYVVQSHKVQGIIMLLAGICDLVDETYSKGTWDSEE"
     gene            complement(6981..7907)
                     /locus_tag="PLG72_04090"
     CDS             complement(6981..7907)
                     /locus_tag="PLG72_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOJ80011.1"
                     /translation="MIISASRRTDIPAFYSGWLINRLRAGYVLTRNPMNHAQVSKVIL
                     SPDIVDCIVFWTKDPLNMLDKLDTIDSMGYKYYFQFTLTPYGRSVERRLRDKEEILKT
                     FCELSDRIGKERVVWRYDPIILNEKFGFEYHKEQFARLCGRLAGHTDQCVISFVDKYP
                     KLRTDAIREISMDEMAGLGSMISSVAGDFGITVKACCEGAFLRELGIEKAHCIDKALI
                     ERVCGYSIDIKKDRNQRDSCGCCESTDIGAYDTCRNGCIYCYANYSDVSVAGNIKRHD
                     PCGELLVGRTGENDKITLREQKSNKDGQLKIL"
     gene            complement(7912..8355)
                     /locus_tag="PLG72_04095"
     CDS             complement(7912..8355)
                     /locus_tag="PLG72_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80012.1"
                     /translation="MLIREVNESDLDSLQELYLHLHEKEKTPETPELNSLWKEIIADK
                     DYHILVGEVDGRIVSSVTVIVIKNLTRCMRPYALIENVVTHSDYRCRGYVGALMQKAV
                     DIAGSSGCYKVMLLTGAKDENTLRFYEKCGFNSKDKTAFIKWIEA"
     gene            complement(8527..8790)
                     /locus_tag="PLG72_04100"
     CDS             complement(8527..8790)
                     /locus_tag="PLG72_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ80013.1"
                     /translation="MEEKYCQSCGMPMGSTDELYGTNQDGTLNGDYCKYCYVDGAFTA
                     DISMEEMIEACVPHVVSANPGMSEDDARNMMRGFFPGLKRWKK"
     gene            complement(8857..9753)
                     /locus_tag="PLG72_04105"
     CDS             complement(8857..9753)
                     /locus_tag="PLG72_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOJ80014.1"
                     /translation="MNRLFEIVYILLGKDKVTAKELAERFEVSQRTIYRDIEALSIAG
                     IPVYTEKGKGGGIGLLPGFTLNRSLLNEQEQEEVISALQALEAVNPRKTAQALTKLSA
                     VFNRKLVNWIEVDFSSWSRLDDGLFDRLKKAIFDKIIVEFDYYSSKGEKTHRKVGPVQ
                     LFFKHQAWYLKGFCLSRQDMRLFKLTRIKDLQLTEHHFNERSFPEALTKDPTDEKSDS
                     PVELKLRIWPEMAYRVYDEFEPRNIRMQPDGSFIAVAVLPEDEWLYGYIMSFGEYAEV
                     IGPPHIREIIRKKLEKTFEKYV"
     gene            complement(10248..11135)
                     /locus_tag="PLG72_04110"
     CDS             complement(10248..11135)
                     /locus_tag="PLG72_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80015.1"
                     /translation="MKRSVGDKIIDAIIVFFMVLVIIAMLYPMWNTLAVSFNDARDSI
                     KGGIYLWPRKWSLYSYQSVFKTDKVFNAFLISVARTIITTILNVFFSGLLAYVLSHKD
                     FVLKKFTTVFMLMTMYINAGLIPQYFLYRNLGLINSFWVYILPAMVGAFNVIVIRTYI
                     NSLPASLSESAKIDGASEMKIYFFVIMPMCLPVLATVALWVAVGAWNDWFTSYIFAPK
                     QSLSTLQYEMMKIISSSMQQGAAQTPDYLQQSQAKSSMVTPNSLRAAMTIVATVPILV
                     VYPFLQKYFVAVNIGSVKE"
     gene            complement(11179..12156)
                     /locus_tag="PLG72_04115"
     CDS             complement(11179..12156)
                     /locus_tag="PLG72_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ80016.1"
                     /translation="MTTNIATSRATIPAKPKQTFFSQAKKQKELVLITIPYIIYFFIF
                     NYLPLWGLTMAFQNYKPYRTFWEQEWVGFKHFQKLFSDPHFYRVLRNTLAMSFINLVL
                     GFVTAIAFALLLNEIPKQLYGFKRVVQTISYMPHFLSWIIVCGLVSNILSMDDGIINN
                     LLLATGLVKEKIHFLGKPEYFWWIVGFTNVWKSMGWNSIIYLAAITSIDPCLYEAAAI
                     DGYGRFGRMWHITLPGIKSTIIILLIMNAGWILNAGFEIQYILGSNGLVLDVSETIDI
                     YVLKKGFAGTAGSGFAFGTAAGMFKTVCSAAILITCNKISSWLGEERLV"
BASE COUNT         3169 a         3171 c         2797 g         3215 t
ORIGIN      
        1 cagcgccctg gccgcctcag gtatgagtcc ctttccccag aaaggcacac ctatccagta
       61 cccgatctcg ccctccgttt cgggcaagtc gaggttgctg gctttcccca ccatgagccc
      121 gatactgccg attgccctgt tgtcctcttt caggcaaaca gcgtaattct catccacggc
      181 cagtacattc ctgattatcc cgaggctgtt ctccacgctc gtatgcggcg gccatcctgc
      241 cataggaccc acccttggat cctttgcgta ttcatacagg ctttccgcat ccgattccag
      301 ccacggacga agtatcagtc tttcagtctg cagttccatt caacacctcg gcatagggta
      361 tctgctgcgg catacagata catatttatc ggaaatgatc cttttggtat tattcaaata
      421 taagcgccgt aaaggtccag ggatcgccgc aaataccggc ggcatccccg taaaatgcgt
      481 ctgatcgccg cgccggacac cgttttattt agattaaaac agattccgca attaatatca
      541 atcgtttttt tcctttatat gctatcctta acatggaggt cggtcatgga ttacaaaaac
      601 catataaagc gggcgataga ttatatagag gagaatctga aaaatgagat cacgatagcc
      661 gattgcgcaa aggtttccgg ttactcaaac taccatttca tccgcgtttt caaagaagcg
      721 accgggctta ctcctgccga ttatatacgc aagcgcaggc tcacagagat cgtaaggaat
      781 atgcgcaagg gtgtgcctgt ttgtgaaata gcgtttgaat acggcttcaa ctccaaggag
      841 aacttcaccc gggcattttt tgcagaacac cacatcctgc cttcggagta caaagctgca
      901 ctgaacagtc tgaagctgta tgaaccgatc atctttgaag cggaaccgtt cgaagtaaaa
      961 ccggagctta catacctcga gccattcgaa ttagtggtct ataagagtga tgaagactgc
     1021 ccgcccaagt tctggaacaa atacaacagc cgaaaatggt ccaaaaagct gacaggcgga
     1081 aaaacctgtg cagacttcgg cgtctgtgtg tggaatcaaa atgaaaataa acttgattat
     1141 ttcataggtg tcagaaaaag cgacgctctc ggagatacgg gcaacaccat cgaacttacg
     1201 gtcccgggcg gcctgtatgc ggtatttgcg acacctcaga gttcacattt cgacttcata
     1261 aacaccatcc accgcacttg ggactacata tacaacgagt ggctgccaaa cagcgggtat
     1321 gaacacacag gcggctgcga gttcgaaaca tacatcgaaa gctcccgcac atacagcgaa
     1381 aaaatctata taccattgaa aagctgagcc tggggaaatc aacccgtgca taacctgtca
     1441 aacatgacga tgtccgtatc agccgtgccg aacgcattca tcagcctccg ccggtgaaag
     1501 ccggggatcg gaacgaaagt acgatcatgg cctgaaaatt gcatccaggg gcccgtcaag
     1561 gatgcttttg taacataaaa tgctgaaaac taatacagga gggaattaaa tgaaaaagta
     1621 ccttaacatt tcatgggacg gtgtgcgcga ttcgacaggt tacctgtttt ccttcgcaaa
     1681 atcactgtcc gcagcggtca aaaacagtcc atattccgaa aaagcggaag atatcgtcgc
     1741 tacatccggc tttgcattca ggatgtgggt cagcagggac ctttgcccgt cggcgacaag
     1801 catctgggac tttgaaagcc agaagccgtg ggtcgagaac ggcggccttc tctgtgatta
     1861 cgtcggacgc tactgggaac aggataacat cgaagaggag agacggctca aagcaatcag
     1921 tatcatcaag gcaagcatcg accgcggcat ccccgctgta tcctgggaca taggtgttcc
     1981 ggaatggggc ctgatcacgg gatatgacga tgattcgcag atgttctccg tgctcgcggt
     2041 caacgatgga aatcccgatc cggcaaatcc tgccctgatg ccctatgatc tgcttggaaa
     2101 acgcgaactg ccgatccttt cggtcctgac tgtcacaggc atatccggaa agacggatga
     2161 acagatcctg caggatacta aaagacttgc ggcgtaccac ctcaaaggcg gagaatggtg
     2221 cgaaaacgca aacggactcg aagcatatcc ggccctgatc aggatattcg atgaggaccc
     2281 aggcctggct tcgtcatgga atgccgaata tttcctcgga acatacggag ctttgaagga
     2341 atatgcttac aggtatttcg aaaaggccgg cgaaaccaaa ctggcggaac tgtaccggtc
     2401 cgtatacgat gcctggatgg aagctttcag gatcaagacg aacgaggacg cttccatacc
     2461 cgaaaccagg gcaaggatcg cttcactgct gaggtccgcg tatgaaaacg aaaaggaagc
     2521 agtcaggata atggagtaag gacagggtgc cctgcaccct gtttcttatt tacatgtcga
     2581 tcagttttat tataaaatcg cccatgtttt tcgcactgtg ccttttgccg tatgtttcct
     2641 gtgcctgcct catctgctcc agtttctcgg gccgtgaata catctgcatt atcacgtcgc
     2701 agatcctcgc gtatctgccg gcatatacag ccagaccgtt gttcatgagg aaataggcgt
     2761 tgcggttttc aactccgggg atcggatcgg taacgataag cggtttccgt ttcgtaagcg
     2821 cttcggacac ggtcagaccg ccaggcttgg ttattatcac atccgccgca tccatgtaaa
     2881 gttccatgtt gtcgacatag ccctcaacga ccacatcctt gctgtagggc gtaccaatga
     2941 tcttctggga gaggcgcgtg ttgcggccgc atatgacgac gatctggaaa tccatcggga
     3001 ccttgtccat gtccgaaagg atctccggga tgtttccgaa cccgacactg ctcgatatca
     3061 ccagcatcgt caatttatcc ttgatgctca gcctggcccg ggcttccgac gctggtatgt
     3121 cgacagagaa ctcctccttc accgggatgc cgaaaggcag cagtttatcg gcatcgatcc
     3181 ctttcctccg ggcctcatgt accaccagtt cgtttgccac cacataataa tcgagtaccg
     3241 tatcctccca gaaaggatgc agcgtaaaat cagttattat cccgaagacc gggatccggc
     3301 catccaggat gccatccttc cgcatctgcg aaatgatgat ggcggtaaaa acatgggtgc
     3361 agatgataac gtcaggcttt ttcaatttta tgtatttcag catcttgctc ttcgaagctt
     3421 ccgcgatcgc atagggcagg aatgttttac gcgttttttc attcccggat accttttcgg
     3481 cgttgtcata catgatcagg tgcagtttcg gggaatgctt agcaatggat gcgtaaccga
     3541 catccactat ttcgcctatc agcttgctca ggtacttata agtatcgagc acggtgcaat
     3601 ccactccctg ggtctccagg tagcgtgcca gagcataact cgtggaattg tggccttgtc
     3661 ctgccgttat cgtcaaaaac aaagcgttca tttcacacaa ctcctcataa tgtgatttcc
     3721 gtttcaatta tactatattt ttcaagtatt aacaaaatcg tcagtgtttt gccgggaaaa
     3781 tgtatacgcc atctcctgcg ggttttaagg cctattgaag tgaaatcata tagtcggttt
     3841 acaggacgat tttttccgga aaagcagtgt gccgcatgat accccacatc ttgtttatat
     3901 atgcaagggt gtagtaactt tcataatggt ggtaatagaa cgcccctttg agcgtcatgt
     3961 ggtaaacgcc gtcttcctcg acgatgaagc ccagtttttt tgccagccac agctcgaatc
     4021 cgtaaacttt ttcaaggggc acaccgaaga aatcctcaaa tttatgcgga tcgacccttg
     4081 tgctgtaggc agaccagaaa aggtaataga tcatcctctg gcgttcggtg aaccgcagcg
     4141 tcagagaagt gggcagcatg ctgttccgga tcctttcctg gtattccttc actgaaaacg
     4201 tgttgatcct gaactggtca cgcaggagcg taacggcaga acagccgaac cccaggtagt
     4261 tgtccctcgt catggatgag tacttcgccc cgggtttgtt tgaaaatgtc cagatagaat
     4321 cccttgtata cccctttgac aggcagtaga gcgtaatgct gtccagaagc ttcctctttt
     4381 cctgtttgcc catggggctg acgccggaac cggcaaaggt gaaatctatg aacgggtata
     4441 ttgccacatg gtcagctccg atcgagaacg ccgtatctat gtccgccttc aggtcatcaa
     4501 aagtctgtcc tggcagcgcg aatatgaagt ccatggatac ggtctcgaac gggacctttt
     4561 ccagcgccgc ctcaacacgc acgggatcga gacctttcct gccaagtatc gaaaggaact
     4621 tgtccccgaa tgactggatc ccgatgctta ttttggtcac acctgcagca cggaggacag
     4681 taagcgtttc ttcgctgaca tcatcggggt gcagttcgag gcctgtccct tcggtaatgt
     4741 caaagtactc gccgatcaca gaaatgatct ctccgatcct ctccgcagcc agggcaggcg
     4801 tcccgccgcc gaaataaagg ctggtcacgc gatttttctc tttcagctgc gaacccgcca
     4861 gacggatttc ccggatcaaa tggtcgatat actcatcaca ggctttctca tcatagagct
     4921 gcctgcaata cgggcaaaac gagcaaagct gcctgcagaa cgggatatgg acatagaggc
     4981 caagaccctt acaatcggca aaatccagct tcccgtcata ttcacttttg aacgtgaatg
     5041 gctgcaactg cctcgtcagc cagacacgcg tcaacctcgc caaaatactc atgttacgat
     5101 tatatcagat gcagtactgc taatgtagta tagtatatat caaattgcag tcatcccagt
     5161 attttctcga atatgtaatc tcttctcttc actccgccgt ctgcagtcga tccgcactga
     5221 tcgacaggat cataccctag tttcctgaac aggttaacag cggttccctg gctccacata
     5281 agtccgtaaa acacggcagc ctctgtcctg catggtattg acagcactca aaagcatctg
     5341 gcttgcaata tgccgccttc gatatcccac atctactgcc agatcaccat aataccataa
     5401 atgggggtcc gatctgcttc gcaggcaata cagcctgccg acgacatcgc ctttcctgtt
     5461 tgtcgccacg atattaaagc agtcactgtt tgctttccct tcatagatag aaagcctgat
     5521 caggttgaca aaacgagggt cttttatatt ccactgcatc agtgctattt tctcagccag
     5581 ttcgttcgaa agaaaatcaa atgtagctat tgttatgata tctcccggaa tttctcccgg
     5641 agacacggcg ttttcctcaa atgatggatg ctgattccca atttccggat ttcccaccgc
     5701 acaacacctc ttttatgcat attgccaggc gccttacctt gatactttca atgctaacat
     5761 tctcacctgt gcgtccatga aaacccttcc ggtacgaatg tttgcataat cccggacaga
     5821 ctgtttccaa tggataaatg acttatagcg tgcctgacac cttaattata tgcaccccaa
     5881 tcatagcagt acaggaaatg ttttattata atttatacta tatactgaaa ccaccgtacg
     5941 gtacgggagg agacaccatg caatatccgg atgcggtcag gacagcgctg gactatatcg
     6001 agcagaacct gctgaaggga aaacctcttc cccggtctga taaattcggc aaagatcata
     6061 ccatgtacgg tgaaaaatac ggcaggggca ttagatgatc tgtgtcgcat actttgaaaa
     6121 tatcgatatg ttcagggatt tggcgaaaac aaaccgccgg atgctgaaat acattattca
     6181 gaacagagaa aggatagaga acagcctgga ttcgctccga tgatgatccc aaaaagtttt
     6241 taccgaattt cgtaattacg tattgccaaa ttgtgaagtt tatgttatta tgatattgcg
     6301 taattacgta atttggatag tgaggtgtga tcatggagag gaattacggc gcagaaatcg
     6361 attcgctgaa tgcggagatc agttcattga agaaggagct gcaggaactc ggcgatgtca
     6421 tcagatcgta tatgcacagc cgtgctaagc aggccgaagc aaaacccatg aaacgagtct
     6481 gccagatgaa ggatatgcat cctgataagc gcctgagcgg gaaaatggag gagttgtgca
     6541 cgctgacaga tgaaaaaggc gttaccggaa tgataactta cctgggcgtt tactgctccg
     6601 gcggcaggca atcgaactgg atcaggaatg aggtctgcac ggacgatctg ctggcactca
     6661 tcgagaacag caccgcagaa aaggtcctga actgcatagg aagcagcgac aggctcaata
     6721 tcctgctcgc actcctgaaa aagccgatgt ctgtggcgga aatcgtgaac gaatgcggcc
     6781 ttaattctac aggccaggca tatcaccaca tgaaaccgct tcttgccgca gacctgatca
     6841 tcgaggacga gaacaacaaa ggagtctatg tggtccagtc ccacaaagtc caggggatta
     6901 tcatgctgct tgcaggcatc tgcgacctgg tcgatgaaac atactcaaaa ggtacatggg
     6961 acagcgagga ataaagccgc ctaaagaatt ttcagctgtc cgtctttgtt tgacttttgc
     7021 tctcttaacg taattttgtc attttcccct gtccggccga cgagaagctc cccacacggg
     7081 tcgtgccttt tgatattgcc ggccacggac acatcgctgt agttggcgta acagtatatg
     7141 cagccattcc tgcatgtatc gtatgcgccg atgtctgtac tttcacagca tccacaggaa
     7201 tcacgctgat tcctgtcttt ttttatgtcg atgctgtatc cgcatactct ctctatcagc
     7261 gccttatcga tacaatgggc cttctctatt ccgagttcac gcaggaatgc accttcgcag
     7321 caggctttta ccgtgatacc aaagtctcct gcaacggatg atatcatact gccaagcccg
     7381 gccatctcat ccatgctgat ctctcttatg gcgtccgtcc tcagttttgg atatttgtct
     7441 acaaagctta tgacacactg gtccgtatgt ccggcaagcc tgccgcaaag ccttgcaaac
     7501 tgctctttgt gatattcgaa gccgaacttc tcatttaaaa tgatcgggtc atacctccag
     7561 acgaccctct ccttacctat cctgtcgctc agttcgcaga aagtctttaa tatctcttcc
     7621 ttgtcgcgca gccttctctc aacggatctg ccatagggcg tcagtgtgaa ctggaagtag
     7681 tatttatagc ccatactgtc gatggtatca agcttatcga gcatattcag cggatccttg
     7741 gtccagaaca cgatacagtc gacgatatca ggcgacagta taaccttgct tacctgggca
     7801 tggttcatcg gattccgcgt cagaacgtaa ccggcacgca gccggttgat cagccacccg
     7861 gaataaaaag ccgggatatc tgtccttcgt gatgcgctga ttatcatgat ctcatgcctc
     7921 gatccatttg atgaacgctg tcttgtcctt gctgttgaac ccgcactttt cgtaaaacct
     7981 taatgtgttc tcatcctttg ctccggtaag cagcatgact ttatagcatc cgctgcttcc
     8041 tgcgatatcg actgcttttt gcatgagggc cccgacatat cccctgcacc tgtaatcgct
     8101 gtgcgtcacc acattttcga tcagcgcata aggcctcatg cagcgcgtca gattctttat
     8161 cactataaca gtgacacttg atactatcct gccgtcgact tccccgacca ggatatgata
     8221 gtccttatca gcgattatct ccttccagag cgagttcaat tccggcgttt ccggagtctt
     8281 ttccttttcg tgcaggtgca ggtacaattc ctgcagcgag tcaagatctg attcatttac
     8341 ttccctgatc agcaaagcga atacctcctg cagcattatg gcgccaggca aggaacacag
     8401 ccacttattt gcatactggg ggacggcttt cctgtcccag gggcgacacc cctcagacag
     8461 gagaaccgtc ccccggtctg agtgaatttt ttcctgcccg tatggcatgc ttgctaccat
     8521 tttactctat ttcttccagc gtttcaatcc agggaaaaat cctctcatca tgttccttgc
     8581 atcgtcttcg ctcatgccgg gatttgcgga taccacatgc ggaacacatg cttcgatcat
     8641 ttcttccatg gaaatgtccg cagtaaatgc cccgtccaca tagcagtact tgcagtagtc
     8701 tccattcagt gtcccgtcct ggttcgtccc gtacagttca tccgttgatc ccatcggcat
     8761 tccgcaggat tgacagtatt tttcttccat agcaatacct ccttatcagt ttatggcatt
     8821 actatagaac acttaatatg tcaactgggt gtcatattaa acatactttt caaaagtttt
     8881 ctccagcttt ttccggatta tctcacggat atgcggaggt ccgatgacct cggcatactc
     8941 gccgaatgac atgatatagc cgtacagcca ctcatcctca ggtaaaacgg caacggcaat
     9001 gaagctcccg tccggctgca ttcggatatt cctgggctcg aattcatcat agacgcgata
     9061 agccatttcc ggccatattc tcagtttcag ttcaacaggg ctgtcacttt tctcatcagt
     9121 tggatccttc gttaaagcct ccgggaaact cctttcattg aaatgatgct cagtaagctg
     9181 caggtccttg atccttgtca gcttgaacag ccgcatatcc tgccttgata ggcaaaaccc
     9241 ctttaaatac caggcctggt gcttgaagaa cagttggacg ggtccaactt tccggtgcgt
     9301 tttctctccc tttgaactgt agtaatcaaa ttccactatg attttatcaa agatcgcttt
     9361 cttcagcctg tcgaacaggc cgtcgtcaag gcggctccag cttgaaaaat ccacttctat
     9421 ccagttgacg agtttcctgt tgaaaacagc cgaaagcttc gtcaaagcct gtgcggtttt
     9481 ccggggattg accgcttcca gcgcctgcag ggccgaaatc acttcctcct gttcctgctc
     9541 gttcagcagg gagcgattaa gggtaaaccc gggaagcagc ccgatccctc cgcctttgcc
     9601 cttctccgtg tatacaggga tcccggctat gctgagtgcc tcaatatccc tgtaaatagt
     9661 gcgctgagat acttcgaagc gctcagcaag ttcttttgcc gttaccttat ctttgccgag
     9721 caatatatat actatttcga aaagcctgtt catttaacca aactacctta ctttcgccga
     9781 caggcgtatt gtatttgcat tgacacatag atatcgcttc gtatctcaat tatactgtac
     9841 gtgacatctg cggtaaagct cggcagccta agtccatctt gcccgacatt tgcacatatg
     9901 tctgccgacg cgcctgttcc gccggcatgc ttgtccacga aggaaatatc catacagtaa
     9961 tcctggatgc atcaggctgc tgcagtacag gctgaggaag caatgtagtg cataatgcga
    10021 taagcacaga cgaatacaac attgctggta atctgagtga caaacggagt tatagagttt
    10081 cctgccagtc cagggatccg aggtaaatta aaacagtgcc aggcacaatg cctgacactg
    10141 cttttagtca tcggttgatt ccatagcgat tcagatactg ccgcaagtaa cagcgctgcc
    10201 gtattgcacg cagtacaggg gatgtatgct taatctgttc cttcaggtta ttccttaaca
    10261 ctgccgatgt tgacagccac aaaatacttc tgaaggaacg ggtatacgac caggatcggc
    10321 acagtggcaa cgatggtcat tgctgcacgc agcgagttcg gagtaaccat gcttgatttt
    10381 gcttgcgact gctgcaggta atccggcgtc tgggcagcac cctgctgcat cgaagaagat
    10441 attattttca tcatctcata ctgaagagtg ctcaaactct gtttcggtgc aaatatatac
    10501 gaggtgaacc agtcgttcca cgcaccgaca gcgacccaaa gagcaacagt agccaggacc
    10561 ggaagacaca tcggcattat gacgaaaaag tatatcttca tttcgctggc accgtctatc
    10621 tttgcagatt ccgacaaaga tgcaggcagc gaatttatat acgtccttat gactataaca
    10681 ttgaacgctc ccaccattgc cggcagtata tatacccaga atgagttgat caggccgagg
    10741 ttgcggtaca ggaagtactg cggtatcaga cctgcgttga tatacatcgt catcagcatg
    10801 aataccgtcg taaacttttt aaggacgaaa tccttatgac tcaatacata cgccagcagt
    10861 cccgagaaga atacgttcag tatcgttgtt atgatcgtac gcgctacaga tatcaaaaat
    10921 gcgttgaaca ctttatctgt tttgaataca ctttgatagc tgtaaagcga ccattttctc
    10981 ggccacagat agatcccgcc ctttatggaa tcccttgcat cgttgaaaga tacggccagc
    11041 gtgttccaca tcggatacag catcgcaatg atgacaagga ccataaagaa cacgattatc
    11101 gcatctataa ttttgtcacc tactgacctt ttcatatcca tatacctcca tgccaaatag
    11161 catcgcaatc acgtccactc agacaagtct ctcttcgccc agccagctgg atatcttgtt
    11221 gcaggttatc aggatcgcag cgctgcacac ggtcttaaac ataccagctg cggtaccgaa
    11281 agcaaaaccg ctgcctgccg ttccggcaaa gccttttttc aggacgtaga tgtcgatggt
    11341 ttcagaaacg tcaagaacaa gtccgtttga acccagaata tactgtattt caaatcctgc
    11401 attgaggatc cagcctgcgt tcattataag aagtatgatg atggtcgatt tgatcccggg
    11461 gagcgttatg tgccacatcc tgccaaaacg gccatatccg tcgatggcag cagcttcgta
    11521 cagacacgga tctatcgaag tgattgctgc caggtatatg atactgttcc atcccattga
    11581 tttccacaca ttcgtaaagc ctactatcca ccagaagtat tcaggtttcc ccaggaagtg
    11641 gatcttttcc ttgaccagcc ccgtggccaa aagcaagttg ttgatgattc cgtcatccat
    11701 cgaaaggatg tttgaaacaa gtccgcagac gattatccac gacaggaagt ggggcatgta
    11761 tgaaatggtc tgcacgacgc gcttgaatcc gtagagttgt ttcggtattt cgttgagcaa
    11821 cagtgcaaat gctattgctg tgacaaatcc cagcacaagg tttataaagc tcatcgccaa
    11881 agtgttccgc aatacacggt agaaatgagg atctgagaac agcttctgga aatgcttgaa
    11941 accgacccat tcctgttccc agaaagtcct gtaaggttta tagttctgga atgccattgt
    12001 aaggccccac aacggcagat aattgaatat gaagaaatag ataatataag gtattgttat
    12061 cagtacgagt tctttttgct tttttgcctg actgaaaaaa gtctgtttgg gttttgcagg
    12121 gatcgtcgcc ctgctcgtcg ctatattggt tgtcatattg ctatcccccc cggctatgaa
    12181 actcaaccga ttctaaattt ttctattttt gctgtatcat atcagaagag gcggccggct
    12241 ctgcggtcgc ctcttcttca tcatccgaaa gaagagaatg atcagtttta cttcttataa
    12301 cgtgccttga tctctttctc gatctctttc tcaagcatcg gatagtcgac gt
//
LOCUS       DAOSCC010000049        12208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3506_length_12208_cov_4.948819, whole genome shotgun sequence.
ACCESSION   DAOSCC010000049 DAOSCC010000000
VERSION     DAOSCC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3506_length_12208_cov_4.948819"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..542
                     /gene="rplS"
                     /locus_tag="PLG72_04120"
     CDS             189..542
                     /gene="rplS"
                     /locus_tag="PLG72_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ80017.1"
                     /translation="MDIIKTLEQEQMRTDLPVLSIGDHVRVHLKVKEGNRERIQVFEG
                     TVIAKKGSGLKETFTVRRVSYGVGVERTIPVHSPKIDTIEIVRKGKVRRAKLYYLRER
                     VGKAAKVKEKLQAKE"
     gene            671..1597
                     /gene="ylqF"
                     /locus_tag="PLG72_04125"
     CDS             671..1597
                     /gene="ylqF"
                     /locus_tag="PLG72_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOJ80018.1"
                     /translation="MNIQWFPGHMAKTRRLLSENLKLVDVVIELLDARIPASSRNPEI
                     DRIVKNKPRLVVLNKSDLADSGISKQWAEWYRSRGIPVIFADSIRGTGLNTLINQLKN
                     LMRERFEKDREKGRRFRTIKTMVVGIPNVGKSTFINKIAGRAVAATGDKPGVTRGKQW
                     IRLHPEVDLLDTPGILWPRFDDKNTALNLAFTGAIRDEILDVVQLGAALAEKLAAEYP
                     ELFFKRYKLQDADGLNGLELLEAAGKNRGCILPGGTIDMKRIADTILDEFRGGRIGAI
                     TLEKPLWDETGDTDGGTETGSKGSKGQDESEQ"
     gene            1539..2255
                     /locus_tag="PLG72_04130"
     CDS             1539..2255
                     /locus_tag="PLG72_04130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ80019.1"
                     /translation="MEEPKQAAREAKVRTNQSNERKHVNEKQILRHEKELQRLNEMYL
                     YEKEAFEQGLEFVAGMDEAGRGPLAGPVVAACVMLPKDCLIEGVNDSKKLSPVKREKL
                     YDIIMEQAISVGIGIVDEKTIDEINILNATKMAMKMAVERVSPKPQLILIDAVELDDM
                     DIPQKAIVKGDMLSISIASASIIAKVTRDRMITEADKKYPVYGFGRHKGYGTKQHIDA
                     IKKYGICPIHRVSFTKHFTM"
     gene            2310..4175
                     /locus_tag="PLG72_04135"
     CDS             2310..4175
                     /locus_tag="PLG72_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ80020.1"
                     /translation="MRIESRGVISAGNSSGISDLVNRLEKGDVIRARVLETSPDEVVL
                     RLSDGTVMKAATVNEPGFRAGEMVNLSVKSKTENQMVLEIVSDPSSNSKPGTDKLLKL
                     LDSLGIEPSKTNLELASELLKYKTVLNAGDMAKALELMKKSDLIDPKTAAFIVSHKID
                     LYGADHEMLARFLNGELKLGQLLDSLAGALERITGMESADNAADDTTASGSIADEESN
                     GTEETENVPDTANAGNTGKSENAVSNQGKGHAAYITEDTELFSHTGPVSHGTGLHGRN
                     GRMAKDLPDGNAHAPAGKDPMNTVVSSRAAIHENTGKSSHALSSDASAVSDTADNAGN
                     DPSTALQESDYVSGRPAFDQPHLPGTEDGPDTAVRNAAGGVINKDIIYTGLHADIGKT
                     VSSSAEDGVVDKGGNDCASPAGKLKDTINDLFVRIDKVLSANDIDAEKIKARIAGLLS
                     ETENLLRSPRMAASNAAENAARSLSLLGETVRMMDLLNSFQVLYYQIPVNLGGNMETA
                     ELYVMKRKANRKRIDPHDTVIFLSLDTQNIGRVEALVDVKGNGISMDLRTENQAVSDF
                     IRSNINSLYSGLSECGYKLVNVSYSVISGPAAPTEQEKLLLAKANYSRVKIDYRI"
     gene            4283..4582
                     /locus_tag="PLG72_04140"
     CDS             4283..4582
                     /locus_tag="PLG72_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOJ80021.1"
                     /translation="MESRKKKIKEAAALRFTPENDAPEIIALGKGAAAENIVEKAKES
                     GVPVHEDAELAHALNMLNIGDEIPPELYEVVAKILVFVGDIDGRFPRPPAYEAKK"
     gene            4579..4947
                     /locus_tag="PLG72_04145"
     CDS             4579..4947
                     /locus_tag="PLG72_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ80022.1"
                     /translation="MNRRQLGKTGEQIAADYLSQNGYTILEKNFRSGRFGEIDIIAEE
                     GSCICFIEVKTRTSDLFGAPSEAVDRNKRQKIRSLALIYLKQKRMGEREIRFDVVEII
                     ARNIKGEIVPEKINLIRGAF"
     gene            4947..5891
                     /locus_tag="PLG72_04150"
     CDS             4947..5891
                     /locus_tag="PLG72_04150"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOJ80023.1"
                     /translation="MLIIDAHCDTALSVYKGGEDGGDLYENSCHLDIKRLVSTGPRVQ
                     FFAAFAHPQEYRNDTLSRVLSILDVVYEAQEKYPDEFAVCKSADDIDKAVSCGKTAAV
                     LSVEGGECLNGELSVLRQLYRLGVRSMLLAWNYRNLLADGAEEGGGAGLTGFGKRVVA
                     EMNRLGMIVDVSHLCEASFDDVMSVTAKPVIASHSNAMAVCRNCRNLTDRQLTDIKTN
                     GGVVGINFYPYFLNNTEKADIDDVIRHIEHICSVAGEDHIGIGADFDGIECTPSGLEG
                     TERIPALFERLLQLNYSETFIEKFAGLNFMRVIRQIMV"
     gene            6239..7519
                     /locus_tag="PLG72_04155"
     CDS             6239..7519
                     /locus_tag="PLG72_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ80024.1"
                     /translation="MEQYLKTLKEDPKSRLSVLEKEYDEFRARGLKLDMSRGKPCKEQ
                     LDLSVEMLAYPYKDKNFKTDSCADVRNYGMVDGLPEAKELFAKMLEVGTNEIILGNNS
                     SLSMMYDTISRAMTFGLYGSPEPWCRLPKVKFLCPSPGYDRHFAICELFNIEMITIDL
                     KDDGPDMDTVEKLAAEDDSIKGMWCTPKYSNPTGITYSDEVVDRLAAMSTAAKDFRIF
                     WDNAYAVHHLTDRPDRLKNILEACKKAGNPDRVFIFASTSKITFPGSGVAMMAASENN
                     ISLIKKQLGIRTICPDKINQLRHLLFFRDIDGIERHMKKHAAILKPKFDMVIGILESR
                     LGGKNIASWSKPNGGYFISFDTLPGCASAVVKLAGEAGVVFTPAGATYPYHKDPQDRN
                     IRIAPTLPPADELRTAMELLCICTEIVSIRKLLA"
     gene            7824..8564
                     /locus_tag="PLG72_04160"
     CDS             7824..8564
                     /locus_tag="PLG72_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ80025.1"
                     /translation="MINKYSNVPLYSQLKQLIIEKIESGDYPVESKIPSEQEFCELYG
                     ISRPTVRQAISELTNNGYLYKEKGKGTFVAKPKSKVDIKNYTGFTDSILDSHDPSQHD
                     IISLRTVRTNDVPLLKDIFGLQGPSANAEFAEIRYVTSENKSVLSYNTSYIPMALFPD
                     LLDDIKDRKPSYDILKGKYPLIPVRSKSTLEIVYTDQTDAQYLQIQPGLPVIRIANIL
                     FSKSGQPVEFIISKYRADKCQLVFEHNK"
     gene            8770..9723
                     /locus_tag="PLG72_04165"
     CDS             8770..9723
                     /locus_tag="PLG72_04165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ80026.1"
                     /translation="MKYAVAFALAFICVYYLIPPLRKFALSIGFVDMPTERKIHTEPV
                     PHLASIGIFIGFMASFLLFSDGMTRRNTGFIAGAFLVLAIGIVDDWYKTHGKDFPALP
                     KAVVQVTAAIIAYFSGFVFTGFTNPFNHGDYVMLPVWLQFLLTVTWIFGVITVINFSD
                     GLDGLAGSLSTISAMTLFIVALAKGQTKSALMSVILVGVAVGYLKYNKPPARIYMGDA
                     GAGFMGYVLGIIALDGAFKQATVLSMFVPILALGVPIFDNLFVVARRLANGKPFYKPD
                     RSQVHYRLLSAGLSPKQVVMFLCLVSVCLSLTSIILLLLKV"
     gene            10357..10719
                     /locus_tag="PLG72_04170"
     CDS             10357..10719
                     /locus_tag="PLG72_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOJ80027.1"
                     /translation="MRFLGNIIWLLLGGIVMSVLWAVSGIVLCITVVGIPFGIQCFKI
                     ANLVLWPFGREIILGNFGVGGLFLNIIWLIVFGWEFAVLHFIIGLLYCITIIGIPFGI
                     QHFKLAKLGILPFGAQIR"
     gene            10941..11915
                     /locus_tag="PLG72_04175"
     CDS             10941..11915
                     /locus_tag="PLG72_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOJ80028.1"
                     /translation="MKNINDYEIPPVLMDFLSYMQTIRGRSANTVQVYFYDLRVFFRF
                     MKLRRNLADKNVPFEEIPINDIDIDFIRTVTLSDLYAFMAFVSNDRDNSSFARARKVS
                     SLKSFFKYLTFKAKLLDTDPAAELESPKLMKRLPRYLNIDESKQLLNSVDGKFSERDY
                     AILTLFLNCGLRLSELVGININNIKGSVLTVIGKGNKERSIPLNQACIRAIEDYMKVR
                     PADGVKDRNALFLSSRKRRISKESVQKIVKKYIIAAGLDPERYSTHKLRHTAATLMYK
                     YGHVDIRSLQELLGHVSISTTQIYTHLDSNQLRTAVDSNPLADFSKEK"
BASE COUNT         3657 a         2603 c         3068 g         2880 t
ORIGIN      
        1 ttgagtcact tcttttttat catgtttggg caattaattt tacaacttac cctgcatggc
       61 agcagcacaa ataaatattt acaaaacgta cttttatgtg ctataatatc tcgcgtgtgt
      121 gtattacggg cggtccgctg gtgcaaacga gcattaatga acgtcctggc aagagaggag
      181 gggcattgat ggatataatc aagacactgg agcaggaaca gatgaggaca gatctgcctg
      241 ttttaagcat aggagaccat gtcagggtac atctgaaggt caaggaaggc aacagggaga
      301 ggatccaggt cttcgaagga actgttattg caaagaaagg ctccggtctc aaggaaacct
      361 tcactgtaag aagagtgtca tatggtgtcg gcgttgaaag gaccattccg gtccattccc
      421 cgaaaatcga cacgatcgag atcgtaagaa aaggtaaagt cagaagggct aagctttact
      481 acctgcgtga aagagtcggt aaagctgcaa aggtcaaaga aaaacttcag gcaaaagaat
      541 gaaaagtata aaggggacca aaggtccctt ttgtatttta tatgtttttt atataatgtt
      601 tttataacag tctgaatcgg acataacaga acatactgga taaacaacta cttgaagagg
      661 acaggtagct atgaacatac aatggtttcc cggtcatatg gcgaaaacga gacgcctgct
      721 gtcggaaaat ctgaaactcg tggatgtcgt catagaactg ctggacgcaa ggatacctgc
      781 aagcagcagg aatccggaaa tagacaggat agtaaagaac aaaccaaggc tcgtcgtact
      841 gaacaaatca gacctggcag acagcggaat atcgaagcaa tgggcagaat ggtacaggag
      901 caggggaatc ccggtgatat ttgccgattc gataagggga accggtctca acacgcttat
      961 aaaccagctt aaaaacctga tgagggaaag atttgaaaaa gacagggaaa aaggccgccg
     1021 tttcaggact atcaaaacga tggtagtggg cattccaaat gtcggcaaat ccacattcat
     1081 aaacaagatc gcaggcaggg ccgttgccgc taccggagac aagccgggag tcacaagagg
     1141 taagcaatgg atcaggctgc atccggaagt cgacctgctt gatacgccgg gtatactgtg
     1201 gcccaggttc gatgacaaaa acactgcatt gaacctggct tttacgggag cgatcaggga
     1261 cgagatcctg gatgtggtac agttgggggc ggcattggct gagaagcttg cggctgaata
     1321 tccggaattg tttttcaagc ggtacaaact tcaggatgct gatggactca atggcctcga
     1381 actccttgaa gcggccggga aaaacagggg atgtatcctg cccggcggga cgatcgatat
     1441 gaaaaggatc gctgatacga tcctggatga gttccgtgga ggcaggatag gtgccataac
     1501 gctcgagaaa cctctatggg atgaaacggg ggatacggat ggaggaaccg aaacaggcag
     1561 caagggaagc aaaggtcagg acgaatcaga gcaatgaacg caagcacgtg aatgaaaagc
     1621 agatacttcg ccacgaaaaa gaattgcaga ggctcaatga aatgtattta tatgaaaagg
     1681 aagcattcga acaggggctt gagttcgtgg ccggcatgga cgaagccgga agggggccgc
     1741 tggccggacc ggtcgtggca gcttgcgtaa tgcttccgaa ggattgcctg atagaaggag
     1801 taaacgattc aaaaaagctg agcccggtaa aaagggaaaa actctacgat atcataatgg
     1861 aacaggcaat atcggtgggt atcggcatag tcgacgaaaa aactatagat gagatcaata
     1921 tcctcaatgc gaccaaaatg gcaatgaaga tggcggtgga gcgtgtcagc cccaaacctc
     1981 agctgatcct tatcgatgcg gtggaactcg acgatatgga tatcccccaa aaggcgatag
     2041 taaagggaga catgctcagt atatccatag catcggcctc catcatcgct aaagtgacaa
     2101 gggaccgtat gatcacggaa gctgacaaaa agtaccctgt atatggtttc ggccggcata
     2161 aaggttacgg gacaaaacag catatcgacg ctataaaaaa atacggaata tgtccgatac
     2221 atagagtgag ctttacaaaa catttcacga tgtgagatgc agtttcggag tcatgggata
     2281 tggccccgga tgttattaag gagtccgtta tgcgcataga aagccgaggg gtaatttctg
     2341 caggaaacag cagcggtatc agcgatctgg tgaaccgtct cgaaaaggga gacgttattc
     2401 gggcaagagt gcttgagaca tcaccggatg aagttgtcct gaggctttcc gacggtacgg
     2461 tcatgaaagc ggcgactgtc aatgaaccag gcttcagggc cggcgagatg gtgaacctga
     2521 gcgtcaaatc aaagacagaa aaccagatgg tccttgaaat agtgagcgat ccgtcatcga
     2581 attccaagcc aggaaccgac aaactcctga aacttcttga ttccctgggc atcgaaccaa
     2641 gcaaaacgaa cctggaactg gcttcagagc tattaaaata caaaaccgtg ctgaatgccg
     2701 gcgatatggc aaaggccctg gaactgatga aaaaatctga tctgattgac ccgaaaacag
     2761 ccgcattcat agtatcgcat aaaatagatc tgtacggggc cgaccatgaa atgctggcaa
     2821 ggttcctcaa cggagaactg aagcttgggc agctgctcga ttcgctcgcc ggagcgctgg
     2881 agcggataac aggcatggaa tcagcagata acgcagctga cgatacaaca gcttccggct
     2941 ccattgccga tgaagagtca aatggtacgg aagaaacaga aaacgtgccg gatacagcaa
     3001 acgcaggaaa tacgggaaaa tcggaaaatg cagtatcaaa ccagggaaaa ggccacgcgg
     3061 catatattac agaagatacc gaattgtttt ctcataccgg cccggtatca catggaaccg
     3121 gcctgcacgg ccgaaacggc cgcatggcaa aagacttgcc ggacggcaac gcgcatgctc
     3181 cggcgggaaa agatccgatg aatacggttg tctcttcacg tgcagcaata catgaaaaca
     3241 ctggtaaatc ttctcatgcg ttatcatctg atgcatcggc cgtctccgat acagcggaca
     3301 acgccggaaa tgatccatcg acagctttac aggaatcaga ctatgtatcc gggaggcctg
     3361 ctttcgatca gccgcatttg ccgggcactg aagacggacc ggatacggcc gtcaggaacg
     3421 ctgccggcgg cgtcattaac aaagacatta tatataccgg tttgcacgcc gacatcggga
     3481 aaaccgtatc ttcatccgcc gaagacggcg tcgtcgataa aggcggaaat gattgcgcct
     3541 cacctgcagg aaaactgaaa gataccataa acgatctttt tgttaggata gacaaagtgc
     3601 tttcggcaaa tgacattgat gcggaaaaaa taaaagccag gatagccggg ctgctttctg
     3661 agacggaaaa tctgctgcgt tcgccccgga tggcagcttc aaacgccgct gaaaacgcag
     3721 cgcgttccct gtcgttgctt ggtgaaacag taaggatgat ggaccttttg aattcattcc
     3781 aggtacttta ttaccagatc cccgtgaacc tgggcggaaa catggaaaca gccgagcttt
     3841 acgtgatgaa acgcaaagcg aacaggaaaa ggatagatcc ccatgatacg gtcatattcc
     3901 tgtctctcga cactcagaac ataggaaggg tcgaggcgct tgtcgatgta aagggcaacg
     3961 gcatcagcat ggatctgagg actgaaaacc aggctgtcag cgatttcatc aggagtaata
     4021 tcaacagctt atattccggt ttaagcgagt gcggctacaa acttgttaat gtgagctact
     4081 ctgtcatcag cggaccggct gccccgaccg aacaggaaaa gctgcttctg gcaaaagcaa
     4141 attacagccg cgtgaaaatc gattatcgca tataggaaac aaagatcagt gtgtatatga
     4201 tacagaaata actttgcata aacacataat aaactgcata tcggcatgtg aactgacaaa
     4261 acataaaagg gcgcagataa ttatggaaag caggaaaaag aaaatcaaag aggccgcagc
     4321 gctgcggttt acccctgaaa atgacgcgcc ggagataata gctctcggca aaggcgctgc
     4381 agcggagaat atagtggaaa aggcgaagga aagcggcgtg ccggtccacg aggacgcaga
     4441 actggcgcat gccctgaata tgctcaatat cggcgacgag attccgccgg aactttatga
     4501 ggtagtggca aaaatacttg tgtttgtcgg cgatattgac ggaaggttcc cgagaccgcc
     4561 ggcttacgag gcgaagaaat gaacaggcgt caactcggaa aaacaggaga acagatagct
     4621 gccgattatc tttcacaaaa tggctatacc atccttgaga agaatttccg ttccgggaga
     4681 tttggcgaaa tcgatatcat agctgaagag ggctcctgca tctgcttcat agaagtaaag
     4741 acaaggacgt ccgacctttt cggcgcgcct tctgaagccg ttgaccgaaa caagaggcag
     4801 aaaataagat ctctggcatt gatttacctg aagcagaaaa gaatgggtga aagggaaata
     4861 cgcttcgatg ttgtcgagat aattgcaagg aatataaaag gagagatcgt gcctgaaaaa
     4921 atcaacctga taagaggtgc tttctgatgc ttataataga cgcacattgc gatactgcat
     4981 tatccgtata taaaggcggg gaagacggag gagatctcta tgaaaacagc tgccatcttg
     5041 acataaaaag gctggtttcg accggtccgc gcgtacagtt ttttgcggcg tttgcccatc
     5101 cgcaggaata caggaacgac accctgtcaa gggtcctgtc catactcgat gtggtatatg
     5161 aggcgcagga aaaatacccg gatgaatttg ccgtatgcaa aagcgccgat gatatagata
     5221 aagcggtttc ctgcggtaaa acggcggcgg tgctgtcggt cgagggaggg gaatgtctca
     5281 acggtgaact gtcggtactg aggcagttgt acaggcttgg agtcaggagc atgctccttg
     5341 catggaacta ccgcaacctg ctggcagacg gggccgaaga aggaggcgga gccgggctga
     5401 ccggcttcgg caaacgggtg gtggccgaga tgaaccgcct cggcatgatc gtagatgttt
     5461 cacacctctg cgaggcaagc ttcgatgatg tgatgtccgt aacggccaaa ccggtgatag
     5521 catcccattc aaatgcgatg gctgtctgcc gtaactgcag gaacctgacc gacaggcagc
     5581 tgaccgatat aaaaaccaac ggcggggtag taggcatcaa tttttaccct tatttcctca
     5641 ataacaccga aaaagcggat atcgacgatg tgatcaggca tatcgaacat atctgttccg
     5701 tcgccggcga agaccacatc ggcataggtg ccgactttga cggtatagaa tgcactcctt
     5761 caggacttga agggacagaa cgcatacctg cactgtttga gcgcctgcta cagctcaatt
     5821 attccgaaac atttatcgaa aaatttgccg gcctcaactt catgagggtt atcaggcaga
     5881 tcatggtatg atgtttccga tgattgcact acaaaaccat gcctaatgaa acctgtctaa
     5941 taaaactatg cttcatagcg gcagttatca taatgccatc ccggaaatga cggcagaaca
     6001 ggcgcgacat gttttttttc aatgttctgc cctctaaaac actccaacgt cattttcaaa
     6061 tatgaatcat cgatttattt atcctgcaaa taaatactca caatgagtta aaaataaata
     6121 aaatatgaaa ttagtcaact ttggagttgc aaaaaccatg caaatataat aaaatatcag
     6181 caatgtatta tatggaggga cgttaaggat ggacttactg ggacaataca ggtgcttgat
     6241 ggagcaatac ttaaaaacac tgaaagaaga tccaaaaagc aggctttcag tattggaaaa
     6301 ggaatatgac gagttcaggg caagaggtct gaaactggat atgtccaggg gcaagccatg
     6361 caaggagcag ctcgaccttt ctgtcgaaat gctggcttat ccgtacaagg ataaaaattt
     6421 caaaaccgat tcgtgcgccg acgtgcgcaa ttacggcatg gttgacggcc ttcccgaggc
     6481 aaaggaactg tttgcgaaaa tgcttgaggt tggcacgaac gagatcattc ttggaaacaa
     6541 ctcgagcctc agcatgatgt atgatacgat aagccgcgcc atgacattcg gcctttacgg
     6601 cagccccgag ccgtggtgca ggctgccaaa ggtgaagttc ctttgcccga gccccggtta
     6661 tgacaggcat tttgccatct gcgagctttt caatatcgag atgatcacaa tagatctgaa
     6721 agacgacggt cccgatatgg acacggtcga gaaactggcg gcggaagacg actccataaa
     6781 agggatgtgg tgcactccca aatacagcaa ccccacaggc atcacatatt ctgacgaggt
     6841 cgtcgacagg cttgctgcga tgagtactgc cgcaaaagat ttcaggatat tctgggacaa
     6901 cgcctatgca gtccaccatc ttacagacag gccggacagg ctgaaaaaca tactcgaagc
     6961 atgtaaaaaa gccggcaacc cggacagggt attcattttc gcatctacgt cgaaaatcac
     7021 gttccctggt tcaggcgtag caatgatggc ggcaagcgag aacaatatca gcttaattaa
     7081 aaagcagctt ggcataagga ccatatgtcc cgataagata aaccagctca ggcatttact
     7141 gttcttcaga gatattgacg gcatcgaacg gcatatgaaa aaacatgcgg caatactgaa
     7201 gccaaagttc gatatggtaa tcggcattct tgaatccagg ctcggcggca aaaacatagc
     7261 aagctggagc aagcccaatg gaggatactt cataagcttt gacacgctgc ccggctgcgc
     7321 ttctgccgtc gtgaaactgg caggggaggc gggggtggtg ttcactcctg caggtgctac
     7381 ctatccctat cataaagatc cgcaggaccg caacatcagg atcgcgccga cactgccgcc
     7441 ggccgacgaa ctcaggacgg cgatggaact gctttgcata tgcacagaaa tagtttctat
     7501 ccggaaactg ctcgcctaaa aacttgcatt aggctgcaaa caatattata attcaataag
     7561 tggtgcattc ttgcaatggt accatacaaa tctcatatct cataagtatc tggcgggatc
     7621 ctgaaatccc gcgatttttt ttcggacaat gtgtaccgtc caggaccggg ctgtgtgcgg
     7681 aagggaaaaa gtccttagct aaatgtactg aaaaatctga tgtaaaatta tacagtttta
     7741 cgttgaattg agcaaaaaaa tcatatagaa ttagtaatga tgtaattatg tgttgctatg
     7801 tttacttgcc tggagggttt caagtgataa acaaatacag taacgttcct ttgtattccc
     7861 aattaaagca gctcatcata gagaaaatag aatcaggcga ttatcctgtc gagtcgaaga
     7921 tcccatccga gcaggaattc tgcgaattat acggaataag caggccgacg gtgaggcagg
     7981 cgataagcga gctgaccaac aacggctatt tatataaaga aaaaggcaag ggcacatttg
     8041 tagcaaagcc gaaatcaaaa gtcgacataa agaattatac cgggtttaca gactcaatac
     8101 tggacagcca tgatccaagt cagcatgaca taatatcatt acgtactgtc aggacaaatg
     8161 atgtgcctct gctgaaagac atattcggat tacagggtcc gtcagcaaat gccgagtttg
     8221 ccgagataag atatgtcacg tccgaaaaca agagcgtcct ttcatataat acgtcatata
     8281 taccgatggc attgttcccg gatcttctgg atgatataaa ggacaggaag ccatcctatg
     8341 atatattgaa gggaaaatat ccgctgatcc cggtaaggtc caaaagcaca ctggagatag
     8401 tgtatacgga ccagacggac gcgcagtacc tgcagataca gcccgggctt cctgtgatca
     8461 ggatagctaa tatccttttt tcaaaaagcg gccagcctgt ggaatttata atttcaaaat
     8521 atcgcgcgga caagtgccag ttggttttcg aacacaataa atagtacgtg catttaataa
     8581 gatataatcg ataacgaaag atttgctaca ggatgatgaa cgatccattg ttaaatatgt
     8641 caaatgtaat gtttgacata tttttttaat attaaaaacg ggcgattcat ggtaaaatct
     8701 atgtggaccg aaacataata caaaagtatg ccatcaggta gattgcttta caaaggaaag
     8761 tgatatgaag tgaaatatgc agtcgcattc gcgcttgctt tcatatgtgt gtactatttg
     8821 ataccgccgc tgaggaagtt tgctttatcc ataggctttg tcgacatgcc taccgagagg
     8881 aagatccaca cggaacctgt tccgcacctt gcgagcatag gcatttttat cggtttcatg
     8941 gcttcattcc ttctgttttc agacgggatg accaggagaa atacaggctt catagcagga
     9001 gccttcctgg tactggcgat agggatcgtc gatgactggt acaagactca tggaaaggat
     9061 ttcccggcac tgcccaaagc cgttgtacag gtgacggcag ccattattgc atatttctcc
     9121 ggtttcgtat ttaccggctt caccaacccg ttcaaccacg gtgattatgt gatgcttcct
     9181 gtgtggctcc agttcctgct tacagtcaca tggatttttg gagtcattac cgtaataaac
     9241 ttttcagatg gcctggatgg actggcggga agcctctcga ccatatctgc gatgacgctg
     9301 tttatcgtgg cattggcaaa ggggcagacg aagtccgcat tgatgtctgt tatcctggta
     9361 ggggtcgccg ttggctatct gaaatacaac aaaccgccag caaggatata tatgggcgac
     9421 gcaggcgccg gtttcatggg atatgtgctt gggataatcg cactggacgg agcattcaag
     9481 caggcgacgg tattgtccat gttcgttccg atacttgccc tgggtgtgcc tatttttgat
     9541 aacctgtttg tcgtcgccag gaggctggcg aacggaaagc cgttttacaa gcctgacagg
     9601 agccaggtcc attacaggct cctttcggcc gggttgagcc ctaaacaggt agtaatgttc
     9661 ctttgccttg taagcgtttg tctcagcctt acttccataa tactattgct gctgaaagta
     9721 taaagccata tggcagacgg caataaactg gagccgcatg gcggaaatgg acttcagaag
     9781 gccggtaaat aatgagcaga aggcataaag aatagatata ttcgtttatt atatggtcaa
     9841 aaccattccg acggcattat aatttagacg taccggatat cctgtacatt gcggcttccg
     9901 gagccgactt cctgaagaaa ggggtggtct gagatgaaga tcgatgcgga ggtggccgtc
     9961 gcctgacggc ctggacaaag tccgccgtgc atccaggcat ggcggacttt taaattttcg
    10021 ctgtaatgcc ttgttttctt tcatatatgc aagcttgaaa ttgacaatac atcaaagctg
    10081 atcatcctgg aacctgaagg atttggacct ggccgggaaa ttacccgtgc tggcttcaca
    10141 tgatacatcg tgcatgttta aggctgcaca gaacaggctt gatcacggaa acaggtcggc
    10201 tggatcgaag ctgatgaatg agcaataatt aaaatgtgaa caagttacat ctctgatttt
    10261 tgtctgccaa taaaaatcag acttaatgtt atatatacag tatgatttat taggtattac
    10321 ttttacctgt aataattcat gaaaggtgaa tcgggaatga gatttttagg gaatataata
    10381 tggctgctcc ttggcggaat agtaatgtcc gttttgtggg ctgtatccgg aatcgttctt
    10441 tgcattactg tagtagggat tccttttggc atacagtgct tcaaaatcgc aaacctggtc
    10501 ctgtggcctt ttggcaggga gatcatactt gggaatttcg gcgtcggagg tttatttctc
    10561 aatataatat ggctgatcgt ttttggctgg gaatttgcgg tcctgcattt tattatcggt
    10621 ttactgtact gcattaccat aatagggata ccgttcggca tacagcattt caagcttgcc
    10681 aaactcggga tactgccgtt tggcgcgcag atacgctgaa cggtaatttg gtatggggag
    10741 gtacgataat tttttataaa taattacacg aaatataaat ttcgcgtaat tgaggggagg
    10801 ctttccggta aacctgggac gatgtataat attgctatac gggactttta aatttataat
    10861 aatttacaga ccgatcatat cattcgtttg gagcgatttg attttgcaca aatgattaag
    10921 ccattaacga ggtcatgcaa atgaaaaata taaacgatta tgaaatacct cccgtgctga
    10981 tggatttcct cagctatatg caaactatcc gcggccgttc cgcgaacact gtacaggttt
    11041 acttctatga tttaagagtt ttcttccgtt tcatgaagct tcgcaggaac ctggccgata
    11101 aaaatgttcc atttgaagaa atccctatca atgacataga tatcgatttc ataaggactg
    11161 tcacgctaag tgatctttat gcttttatgg cttttgtaag caatgaccgt gacaattcat
    11221 cgtttgccag agcccgcaag gtttcgagct tgaaatcttt tttcaaatat cttacattta
    11281 aggcaaaact tttagatacg gaccctgcgg cagaactgga atcgcccaaa ctgatgaaac
    11341 gtctgccaag atacctgaac attgatgaaa gcaagcagct gcttaactcc gtagacggca
    11401 agttttcaga gcgcgactac gccatactga ctctgttcct caactgcgga ctccgtctgt
    11461 ccgaacttgt cggaataaac ataaataata taaagggttc ggtcctcact gtcatcggaa
    11521 aaggaaacaa ggaacgcagt attcccctca accaggcttg tataagggct atcgaggact
    11581 atatgaaagt caggcctgca gacggggtca aggaccgtaa cgcattgttc ctgagcagcc
    11641 gcaagcggag gatcagcaag gagtccgtgc agaagatcgt caagaaatac ataatagccg
    11701 ccggtctcga tccggagcgg tattccaccc ataaactgcg tcatactgca gctacgctga
    11761 tgtacaagta cggacatgtt gacataagat cgctccagga actgcttggc catgttagca
    11821 tctccaccac gcagatatat acccatctcg acagcaacca acttaggaca gctgtagaca
    11881 gcaatccgct tgcagacttc agcaaggaaa agtaaaaatg aaggtttact taatatgacg
    11941 agttaattat aatattatag ttgtaccgac gcttcagtta atgtgctgtc ggtacggtgg
    12001 aaccgttcgc ttgcaaaatg agtctcattt ataggctcaa gcgctgcggc gcggtatatc
    12061 gggcaaaata ctgccagtat ggcatttata atgctatacg gaccgtgata aggacctgac
    12121 atgagtaata tcgatataag tatagggata ataagttaac gaggtcatta caaatggaat
    12181 caagaaaatt ttactttata ttgacgat
//
LOCUS       DAOSCC010000050        12071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3547_length_12071_cov_4.968375, whole genome shotgun sequence.
ACCESSION   DAOSCC010000050 DAOSCC010000000
VERSION     DAOSCC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3547_length_12071_cov_4.968375"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLG72_04180"
     CDS             complement(<1..372)
                     /locus_tag="PLG72_04180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80029.1"
                     /translation="MDIEIREVNDEFEEWASVIRNSFATVAEEFRITRENAPTNPAFA
                     EADSLEQMKVKGVSMYGAYLDWQRVGFVAIERADDDRWYMERLAVLPEYRHRGIGRAL
                     MDFVFETVRKRGGKKVSIGIIN"
     gene            complement(670..1410)
                     /locus_tag="PLG72_04185"
     CDS             complement(670..1410)
                     /locus_tag="PLG72_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80030.1"
                     /translation="MKKPCFLICLVASMLLAAGCAAGTNNVPEETAAFKDPQDIRVPA
                     DGTVNGDQPDQGQADHGDNIGKPDENISGPAENTGMQEESAGKAGENPEQAGRNGDRD
                     GEEASDSAVAVVPWPSPHETVNDLEGVSMTIKEGTVSPTGLTVILENTSEKTYIYGED
                     FILEKKIEGKWYKLQYTFEGNFGFILIGYNLEPGSKSEWTVRWDLPYGTLNEGEYRIL
                     KDVIFSKGDGKYDKYWLAAEFAITCPEI"
     gene            complement(1496..1678)
                     /locus_tag="PLG72_04190"
     CDS             complement(1496..1678)
                     /locus_tag="PLG72_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6472 family protein"
                     /protein_id="HOJ80031.1"
                     /translation="MKKVSNCEHCVNYVYDDEYCSYVCTVDLDEDEYARYLQRSYDNC
                     PYFKLDDEYGTARKQM"
     gene            complement(1725..2153)
                     /locus_tag="PLG72_04195"
     CDS             complement(1725..2153)
                     /locus_tag="PLG72_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ80032.1"
                     /translation="MFLYVFSIIMIVASNTMYHICQKSTPERVNPFVALLVTYATAII
                     MTAVTLIFYKPEKGLIESIKQLNWTSIVLGISIVGLELGYLLAYRAGWNISVGSLVAN
                     ILLALVLIPIGIILYHEKFELSKVIGSVFCIVGLILINRK"
     gene            complement(2280..3239)
                     /locus_tag="PLG72_04200"
     CDS             complement(2280..3239)
                     /locus_tag="PLG72_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOJ80033.1"
                     /translation="MEIPFELRAALERQLQGINHGQLVRDSRNISFRYRTRGGTGERL
                     LTNDSEALSYAISRMPATYGAVRTALEKTLACVDCRPRTLLDAGAGTGAASWAAAGLM
                     DLESIICLEREMAMRTLGARLMEDGPRVLRETKWIEHDLETGEIPWKADLVIASYVLN
                     EMTGDARKRAARKLWESAGMILLLVEPGTPAGFSHLKEVRGMLAGQGAFIAAPCPHSA
                     DCPMEADDWCHFTCRVARSRLHRQMKGGEAPFEDEKFTYMAFVREACGNAGMRILRHP
                     QVRGGHIILRACTKDGIEDITLTKKDGERYKKARKAGSGDELC"
     gene            complement(3396..3545)
                     /locus_tag="PLG72_04205"
     CDS             complement(3396..3545)
                     /locus_tag="PLG72_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80034.1"
                     /translation="MSDRLKVLAKIVIGLTFIQVTGYAARKAVFLCIDRTLLSDTVVQ
                     QRYCQ"
     gene            complement(3611..4219)
                     /locus_tag="PLG72_04210"
     CDS             complement(3611..4219)
                     /locus_tag="PLG72_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80035.1"
                     /translation="MEYICESSENEMIALFLQTEIRSERYRDEILQALSGFGARESVI
                     LEPDIGSDGENVLRRKVLARFRGYGEDREIFEGFPEDVRWIWAYVTASELEKVKYIDY
                     DYWVELSGGSRLAKDAAVNIRNGVEIFGVSNDGFLAGAKALLDGAAFPPMILVSDNEE
                     TIVLEGHFRLTVYMLEPVCAKDKLKVMIGYTSKEKLMKWSLY"
     gene            complement(4453..4596)
                     /locus_tag="PLG72_04215"
     CDS             complement(4453..4596)
                     /locus_tag="PLG72_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80036.1"
                     /translation="MDIGIIGGADGPTAIYVSSGGFFYIALAVIVLLIAVVGFIVYKK
                     RKK"
     gene            complement(4601..5077)
                     /locus_tag="PLG72_04220"
     CDS             complement(4601..5077)
                     /locus_tag="PLG72_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOJ80037.1"
                     /translation="MITEEFRSVVESFNDKLAGIPGEITGIRLSEDKWSLKEIIGHLI
                     DSASNNHQRFVRLQFGDLVNFPPYEAESWVRVQKYNEMDWKELIALWYNYNRILLNVI
                     GCMDDKAADNVWKAGERESTLGFLVKDYYRHLKWHIDQFGNRLSEINGSKQDELEG"
     gene            complement(5442..5837)
                     /locus_tag="PLG72_04225"
     CDS             complement(5442..5837)
                     /locus_tag="PLG72_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ80038.1"
                     /translation="MDYKIKLNSFYLCVKDLKRAVSFYEQLLDQKVEDNGSLFVINGI
                     RFWLFDYQAANDSRVVFGHNCLPSFEVNDIRALMKKLDELQAQIVFPLTRIGDNWVLE
                     FRDPEGNDIEVYSKYRPLDEDPDENYQFY"
     gene            complement(5976..6746)
                     /locus_tag="PLG72_04230"
     CDS             complement(5976..6746)
                     /locus_tag="PLG72_04230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ80039.1"
                     /translation="MQKDEILKTNKSYWDTYADFWFGTTALPEYGVYFVTEDELHLFG
                     DVTGKKLLEIGCGSGHSLKYHADRNAGELWGVDISRKQLENAEAYLKEHGYTAKLICA
                     PMEAELDIPKDYFDYVYSIYGIGWTTDLQGTFRKIASYLKKDGIFIFSWHHPLNYCVA
                     WSCGERRDVIENGKLVFMKSYFDESYFRMPVHESEVILCNRKISTYINALVQAGFVIE
                     HMVEQTDRETMEAEGDISDKARKAKMLPISFCIKARKL"
     gene            complement(6872..7237)
                     /locus_tag="PLG72_04235"
     CDS             complement(6872..7237)
                     /locus_tag="PLG72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80040.1"
                     /translation="MDVNIRKAEKKDLEYILALYSQPDMDNGEVLDIEEAERIFDKMG
                     RYPDYSVYVAELDGNIVGTFALAIMDNLAHMGMPSGLIEDVVVSECFQGRGIGKLMMK
                     RAIEICRKKVVTRQRFRAT"
     gene            complement(7256..7894)
                     /locus_tag="PLG72_04240"
     CDS             complement(7256..7894)
                     /locus_tag="PLG72_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80041.1"
                     /translation="MNTLTGKIGSVLTGAAVLSFAVSMLVTEGVFASCLSSIFIAIGF
                     IPFMCSIAAINKKTENKAVGYTGIAFCVIYAAIIFLVYYAECTTVRLDPSLSNEALSI
                     ISFGHTGSLFFNYDLLGYSFMALSTFLMAFLVEPVCRKDRIFRWMLWIHGIFFVPCFF
                     IPMLSVFSAGTSDTVGIVLLEIWCAYFLPICVLGYEYFKTKGMQLGAISKQI"
     gene            complement(7930..8553)
                     /locus_tag="PLG72_04245"
     CDS             complement(7930..8553)
                     /locus_tag="PLG72_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ80042.1"
                     /translation="MALKKYDCLLFDIGGVLAELCSAPKLLEWMGNRVSYEEMRILWT
                     HSDAVRNYEKGLITSEDFAKRVVEELQFNISAERLLEEFVLFIKCLYPGVPEVLKDLE
                     KEYVTATLSNTNELHWDRLCNEFGFDKIIKRNILSFRIGLMKPDPEIYRYAISKLSCE
                     PSRILFFDDNRINVEAARNAGIDAIQVSGFADVKQKLFEIGIIEKHE"
     gene            complement(8579..9154)
                     /locus_tag="PLG72_04250"
     CDS             complement(8579..9154)
                     /locus_tag="PLG72_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ80043.1"
                     /translation="MDVRKLIEILSVAEKLKNNTRHSWTSSGRQESVAEHSWRLSMMA
                     YLVKDEFPDADINKVILMCIFHDIGEVFTGDIPAFSKTDSDELDERQKLQQFLDSLPE
                     PYREELFGLFEEMYAQETTESRIFRALDRMEAIIQHNEADISTWLPLEYELNLTYGEK
                     EAEFSEYMKRLKQAANEDTVRKIRCAEESAS"
     gene            complement(9211..9510)
                     /locus_tag="PLG72_04255"
     CDS             complement(9211..9510)
                     /locus_tag="PLG72_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOJ80044.1"
                     /translation="MSDFPALSFLRHTGEDLYSIGVRSVSKWNAVRAVAGYYGIDTDM
                     IAAFGDDMNDLEMVRNCGTGVAVRNAVEEVKRAADYICGSNDDDGVAKWIEEFIL"
     gene            complement(9526..9942)
                     /locus_tag="PLG72_04260"
     CDS             complement(9526..9942)
                     /locus_tag="PLG72_04260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80045.1"
                     /translation="MGDIVLKLATHDDLDILAGMNKQLIEDEKHDNKMDVSQLRDRMR
                     GFIDGNYNAYLFLKDGKVAGYALVDMSRDPLYIRQFFISREYRRRGYGRAALTGLLKL
                     LGADRADIEVLWWNETGIKFWKSMGFKERSIYMRLG"
     gene            complement(10007..10561)
                     /locus_tag="PLG72_04265"
     CDS             complement(10007..10561)
                     /locus_tag="PLG72_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOJ80046.1"
                     /translation="MDPQFVNEYTVSKLRFLEWAKRPVTPDVTQYLWIAMAVIIPAFA
                     VISAAYDDIVYLLLYAFLELFCVFMIFFRKRLLLARQFKALTIVQGKDEWERRIELGD
                     KIKVTDGNSAIEYEWAQVKEFIADRDYLILVMARRLGLRLDRSGFTKGTGESFIEYMK
                     NEHAGIPIKVRKQKKRLVLGLYIR"
     gene            complement(10543..10788)
                     /locus_tag="PLG72_04270"
     CDS             complement(10543..10788)
                     /locus_tag="PLG72_04270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ80047.1"
                     /translation="MTEACRAVLDFAFNKIGFAKVHARHHADNPANGRVMQKSGMRFI
                     GMKYREVPDCERISGDYCLYEATSEDWKEDMKWIPSL"
     gene            complement(10809..11600)
                     /locus_tag="PLG72_04275"
     CDS             complement(10809..11600)
                     /locus_tag="PLG72_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ80048.1"
                     /translation="MKTNGVKTVVKMIVMDLDDTLLRSDKTISDYTVSVLKKCRDRGI
                     RTAYATARSKQTVSRFFERFAPDVFIGYGGAVSFAGEKVISRFAIPAEIGSKLIDRCL
                     REPEIPYVHACNETVAHANSLDSPNSEIVHYECVDFSQLSDVSYLKISLISRNPDVVE
                     RIASEFPTLDLLRYTGEDLYRFADRRAVKWNAVKAVAGYFNIDTDTVAAFGDDVNDTE
                     MIKNCGIGVAVGNAVEEVKAAAKYICDTNDNDGVAKWLEEYILAD"
     gene            complement(11759..>12071)
                     /locus_tag="PLG72_04280"
     CDS             complement(11759..>12071)
                     /locus_tag="PLG72_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80049.1"
                     /translation="LTASRFERILKWIFFSLAAAELLLCLIFSGQPTAGILAALSALM
                     VGVSAGYPNMIWAVNKFRISMYTDSDDITPNDFWSYGHKVSYWILFGVSVVLLIVSLS
                     W"
BASE COUNT         2991 a         3312 c         2368 g         3400 t
ORIGIN      
        1 attgatgatg ccgatggaga cctttttccc gccacgcttc ctgacagtct cgaaaacgaa
       61 atccatcaaa gccctgccta tacccctgtg cctgtactca ggcagcaccg cgagcctttc
      121 catgtaccat cgatcgtcat ctgctctttc tatcgcaacg aatccgaccc gctgccaatc
      181 caggtatgca ccatacatac tgactccttt cactttcatt tgttcgaggg aatcagcttc
      241 tgcaaatgca ggattggtcg gggcattctc tctggttatc ctgaactctt ccgccactgt
      301 tgcgaaagag tttctgataa cggacgccca ttcctcaaac tcatcattta cttctctgat
      361 ttcgatatcc atgttacacc cctgtaccta ttagctgcac agttgccagc gcaagtatgt
      421 tattcaatta ctacattaaa tataagttta tgtcaacact cattatttct tccaggtgtt
      481 cgagcagata tgtccctgat catggccgcc ttaatgcaat tgcaaccgga ttgtcgaaga
      541 tccatgaaag aaagtgatca aggaagggaa atgtgcagag gcaaatatat ccctgcaata
      601 tgagatttgc cttggattat gcgttataaa accaatcatg cagatatccc ggatacatat
      661 ccataatgct catatttccg gacaggttat ggcaaactca gccgccagcc agtatttgtc
      721 gtacttgccg tcgcctttgc tgaatataac gtctttcaat atccggtatt caccttcatt
      781 gagagtgcca taaggcaaat cccacctgac cgtccattca cttttgctgc cgggctccag
      841 attgtaaccg atcagaatga acccgaaatt gccttcaaaa gtatactgga gtttgtacca
      901 cttgccctcg atctttttct ccaggataaa gtcctccccg tagatatacg ttttttcaga
      961 ggtattttcc agtatgacgg tcagccccgt cggcgagacc gttccttcct tgatggtcat
     1021 ggaaacgcct tcaagatcat tgaccgtctc gtgcggtgag ggccagggta caacggcaac
     1081 agcgctgtcg gacgcttctt cgccgtcccg atcgccgttt cggccagcct gttcaggatt
     1141 ttctccggct ttgcccgcgc tttcctcctg catgcctgtg ttttctgctg gtccgcttat
     1201 attttcatcc ggcttgccga tgttatctcc atggtcagcc tggccctggt caggctgatc
     1261 gccgttaaca gtaccatctg ccggtactct tatatcctgc ggatctttga aagcggccgt
     1321 ttcctccggg acgttattcg tgccggcggc acatcctgct gccaacagca tgctggctac
     1381 caggcaaatc aaaaaacatg gttttttcac acgatcaccc ctatgttttt tgacttgttt
     1441 aaatacaaaa aggttctgtt attaccatat gctccctggc tgtaaaacct gcggatcaca
     1501 tctgcttcct tgcggttccg tattcatcgt ccagcttgaa atacgggcag ttgtcgtaag
     1561 acctttgcag gtatctcgca tattcatctt catcgagatc cacggtgcat acataactgc
     1621 aatattcatc gtcataaaca taattcacgc aatgttcgca gttcgatact ttcttcatgg
     1681 cgacccacct ccatggcgaa cctggatttc agatcaaaag gcacctactt cctgttaata
     1741 agtatcaggc cgacgatgca gaagacagaa ccaatgacct tggacagttc aaacttctcg
     1801 tgatacagta ttatccctat cgggatcagt acaagggcaa gcaggatgtt ggctacaagc
     1861 gatccgacgc ttatgttcca gcctgccctg tatgccagga gataaccgag ttccagccct
     1921 acgatggaga tacccagtac tatgctggtc cagtttagct gttttatcga ttcgataagg
     1981 cctttttccg gtttgtaaaa gataagcgtc actgctgtca ttatgatcgc cgtggcatat
     2041 gtcaccagaa gggcaacaaa cggattcacc cgctcaggtg tcgacttctg gcagatatga
     2101 tacatggtgt tcgacgcgac gatcatgatt atcgagaata catacaaaaa catatgatca
     2161 actgcctcgc tttaatgtca ttttaccctg cacaggcttt tcctgctggt ttatccaaaa
     2221 ttacatgttg ctgtattttt attatatcag aatactccat tcggcagaat acgccggaat
     2281 cagcacaatt catcccctga accggctttg cgtgcttttt tgtacctttc gccgtccttt
     2341 ttggtcagcg ttatatcttc aatgccatcc ttcgtgcagg ccctcagtat tatgtggcca
     2401 ccgcgcacct gcgggtgccg caggatcctc atgccggcat taccgcacgc ctcacgtacg
     2461 aatgccatat aagtgaattt ttcatcttca aacggcgctt ctccgccctt catctgacga
     2521 tgcagccggc ttctggccac acggcatgtg aagtggcacc agtcatctgc ttccatcggg
     2581 cagtcggcag aatgaggaca cggcgccgcg ataaacgccc cctgccctgc gagcatgccg
     2641 cgcacttcct tcagatgtga aaaacctgca ggcgtccccg gttcgaccag gagcagtatc
     2701 attcccgctg attcccacag tttccgggct gcccgcttcc ttgcatcccc ggtcatctcg
     2761 ttgaggacat atgatgcgat gaccagatcg gccttccacg ggatttcacc tgtttccagg
     2821 tcatgttcga tccatttcgt ttcacgcagc acgcggggcc catcctccat gagcctggcg
     2881 cccaatgtac gcatcgccat ttctcgttca aggcatataa tactttcaag gtccatcaaa
     2941 ccggcagccg cccatgaagc ggcgcctgtc cccgccccgg catcaagcag cgtgcggggc
     3001 cggcagtcaa cgcatgcaag tgtcttttcc agtgccgtgc ggaccgctcc gtatgttgcc
     3061 ggcatccttg atatggcata tgacaaagcc tcgctgtcat tcgtaagcag acgttccccg
     3121 gtaccgcccc gggtcctgta cctgaagctt atattccggg aatcgcggac aagctgtccg
     3181 tggtttatgc cctgcagctg ccgttccagc gccgcccgca gttcaaaagg tatttccata
     3241 tgtctgcatc acctgttgtg aaaatcctga tctattgcgt aaatcctgtt ctcattcatc
     3301 gtagtcgtga tctattctcc gtcataataa ccgacctggg aatacggaaa gcgaaacgcc
     3361 tttttttacg gcaatgatta cgccgatcgc cagcattact gacaatatcg ctgctgaaca
     3421 acagtgtcgg acagcaatgt cctgtcgata cagaggaaca cggctttcct ggcagcatat
     3481 ccggtgacct gtatgaacgt caacccgatg actattttgg ccaaaacctt tagcctgtca
     3541 ctcataacaa tcccccgtgg aaccacgtta taaaaattgg gtaaatcttc agccatataa
     3601 aattcatgat tcagtacaga ctccacttca tcagtttttc tttggatgtg taaccgatca
     3661 ttaccttcag cttatctttg gcgcaaacag gttcaagcat atatacggtc agcctgaagt
     3721 ggccttccag tacgatggtt tcctcgttgt cggacacgag tatcatcggc gggaatgctg
     3781 ccccatccag caaagccttt gctccggcga ggaaaccgtc attgcttacg ccaaatatct
     3841 caacgccatt tctgatgtta acagcagcat cctttgccag cctcgaacct cctgacaact
     3901 ccacccaata atcataatcg atatatttga ctttctccag ttcgcttgcc gtcacgtatg
     3961 cccagatcca gcggacatct tccgggaaac cttcgaatat ctccctgtct tccccgtatc
     4021 ccctgaacct ggccaatacc tttcttctca ggacattttc accatctgaa ccaatatcag
     4081 gctcaagtat tacagattcc cgggcgccaa aaccggacag agcctgtaaa atctcgtccc
     4141 tataccgctc cgacctgatc tcggtctgca aaaacaacgc gatcatctca ttttccgatg
     4201 actcgcatat atattccatt aagctgcccc ctgttatctg gtttaatttt gatgcagtgc
     4261 tgcatcactg ttccggtctc tgacattcag gacagtaata gatattgcct ccaaggtatg
     4321 cttcccggac taagtcttgc tttcaggcag ttcgatcata taagcccgtc ctttctgtac
     4381 tcaggtgagt tgataagcac ctcctgaggc tgccaggttt caggcagcat tttcaacagg
     4441 gatgccatat gatcatttct ttcttttttt gtacactata aaaccgacaa ccgctataag
     4501 cagaacgatc actgcaagag caatgtaaaa aaagccgcct gacgatacat atattgccgt
     4561 cggcccgtcc gctcccccaa tgatgcctat atccacctta ttatccctcc agttcatcct
     4621 gtttcgatcc atttatctcg ctgagcctgt tcccgaactg gtctatgtgc catttcaggt
     4681 gacggtagta atccttgacc aggaaaccga gcgtggactc tctctcaccg gccttccaca
     4741 cgttatctgc tgctttgtcg tccatgcagc cgatgacgtt cagcagtata cggttgtaat
     4801 tataccaaag cgctatgagc tccttccagt ccatctcatt gtatttctgc actcttaccc
     4861 atgattctgc ttcatatggc gggaaattga ccagatcccc aaactggagc ctgacaaaac
     4921 gctgatggtt gtttgacgct gaatcgatca gatggcctat gatctccttc agcgaccatt
     4981 tgtcttcgga cagcctgatg cctgttattt cgccgggaat cccggcaagc ttgtcgttga
     5041 acgactcgac gacgctcctg aattcttctg taatcataat accgaccccc aaatcacatt
     5101 tgaaattatt aagtgttaag gacgaacgtt catgcagaat actgctgcca cattttgtat
     5161 tttacgcacc cgcatttact tcacaaacct gcatacatat cctgaagttc cacagcccga
     5221 gcatctgagc ggccaatcat tataattata tactcttgtt aatcttttct ccagttcttt
     5281 cctgaattcg gtccgaaaga tacctggccg catgaaacgg atgatctctt cagtgacatt
     5341 agcatttcgg ttttctcact caaaaacact actcccaact ttcaaataaa ttctgtagaa
     5401 gataatgcat gtagaaagca catatggaaa cattgaagat atcagtagaa ctgataattt
     5461 tcatccgggt cttcgtcaag gggacggtat tttgaataga cctcgatatc attcccttcc
     5521 gggtctctga attcaaggac ccagttgtcc cctatacggg taagcggaaa tactatctgt
     5581 gcctgcaatt catcgagttt tttcatcaag gccctgatat cattcacttc aaaactcggc
     5641 aggcaattat gcccgaacac aacacggctg tcatttgcag cctggtagtc aaacagccag
     5701 aaccttatgc cattgatgac aaacaacgaa ccattgtctt cgaccttctg atccagcaac
     5761 tgctcgtaga agctgactgc ccgtttcaga tccttaacac aaagatagaa tgagttgagt
     5821 ttgattttgt aatccatata ccccccaata agtgccgaac ctggcatgtc attttatcgc
     5881 caatatacgc ccaatataca ctgcaaaaac tgacaacagc atgtcatata taaatcaggc
     5941 aaatgcagaa gttcggggca atcgttattt tcattttaca gttttctggc cttgatacag
     6001 aaagaaatgg ggagcatttt tgcttttctg gctttatcgc tgatatctcc ttccgcttcc
     6061 atcgtttccc tgtctgtttg ctctaccata tgctcgatca cgaatcccgc ctgcacaagg
     6121 gcgttgatat acgttgatat cttccggttg cacaggatca cttcactttc gtggacaggc
     6181 attctgaagt atgattcatc aaagtagctt ttcataaata ccagtttacc gttttcgata
     6241 acatccctgc gttcgccgca ggaccatgcc acgcagtaat tcagcggatg gtgccagctg
     6301 aaaatgaaaa taccgtcttt tttcaggtaa gacgcaattt ttctgaatgt gccctgaagg
     6361 tctgtcgtcc agccgatccc atatatcgaa taaacgtagt cgaaatagtc cttcgggata
     6421 tccagttcgg cttccatagg cgcacagatc agttttgccg tataaccatg ttcctttaag
     6481 taagcttcgg cgttctctaa ttgcttgcgg gagatatcca cgccccacag ttcacctgca
     6541 ttcctgtctg cgtggtactt cagggagtgg ccgctgccgc acccgatctc cagcagtttt
     6601 ttgccggtaa catcaccaaa caggtgcaat tcatcttctg tcacaaagta cactccatac
     6661 tccggcagcg cagtagtacc gaaccaaaaa tccgcatatg tgtcccagta cgatttgttt
     6721 gtcttaagta tttcatcttt ttgcatagca tgcgtacctt ccttccgtcg atacataggt
     6781 cttatataaa tatcacagca attccatact gaaactgtat ccatgcttcc tgaaaccgat
     6841 tccttcgtaa aaccggtgcg ccttttcctc ttcaggttgc tcgaaagcgc tgccttgtaa
     6901 caaccttttt cctgcatatc tctatggcgc gtttcatcat gagtttgcct atccccctgc
     6961 cctggaagca ctccgaaacg acgacatcct ctatcagccc ggaaggcatg cccatgtgtg
     7021 ccagattatc cattatcgcc aacgcaaatg tcccaactat gtttccgtcg agttctgcga
     7081 cataaacact gtagtcgggg taccgcccca ttttgtcgaa tatcctttcc gcttcttcaa
     7141 tgtccaggac ctctccgttg tccatatccg gctggctgta caaagccagg atatactcaa
     7201 ggtccttctt ttcagcttta cggatgttaa catccattca aaccgacctc acccattaaa
     7261 tttgcttact gattgcacca agctgcattc ctttcgtttt gaaatactca tagcctaaaa
     7321 cacatattgg taagaagtaa gcgcaccata tctccagcaa tacaatccca acggtatcgc
     7381 ttgttcccgc cgaaaataca gataacatgg gtatgaagaa acacggcaca aagaaaatac
     7441 catggatcca aagcatccac ctgaatatcc tgtcttttct gcaaaccggt tcaacgagaa
     7501 atgccattaa gaacgtggac aaggccataa atgaatatcc gagcagatca taattgaaaa
     7561 acaggcttcc ggtgtgtcca aaacttatga tagacaaagc ttcattgctc aatgaaggat
     7621 ccagcctcac tgtggtacat tcagcatagt acaccaaaaa tataattgcg gcatatatga
     7681 cgcagaacgc tattccagta tatccgacag ctttattttc ggtttttttg ttaattgctg
     7741 ctattgaaca cataaaaggt ataaaaccta ttgctataaa aatactggac aggcagcttg
     7801 caaacacccc ctctgtcaca agcatcgata ctgcaaatga caaaacggct gcgcctgtca
     7861 atacagaacc gattttaccg gtcagggtat tcattttcaa cctccctggt tcacagcatt
     7921 ttataaattc tattcgtgtt tttcaatgat accgatttca aacaacttct gtttcacatc
     7981 ggcaaagcct gatacctgga tcgcatcaat accggcattc ctggccgctt cgacatttat
     8041 cctgttatcg tcaaaaaaca aaatcctgga cggttcgcat gacaacttgc ttatcgcata
     8101 cctgtatatt tccgggtccg gtttcatgag cccgatgcga aatgaaagga tatttcgctt
     8161 tattatcttg tcaaaaccga attcattgca tagcctgtcc cagtgaagtt cattcgtatt
     8221 tgagagcgtc gcagtaacat attctttctc cagatccttc aacacctcag gcaccccggg
     8281 atacaggcat ttgatgaaaa ggacgaattc ctcaagaagc ctttccgcac tgatattgaa
     8341 ctgcaattcc tccactacac gttttgcgaa atcttctgat gtaatcaaac ccttttcata
     8401 gtttctgaca gcatcagaat gcgtccacaa aattctcatt tcttcatagc tgacccggtt
     8461 acccatccat tccaggagct tcggagcact gcaaagctcg gccagtacac cgccaatatc
     8521 aaataacagg caatcatatt tcttcagcgc cataatttgc tccccctgtt tgtacgactt
     8581 acgatgccga ttcctcagcg catcgtattt ttctgaccgt gtcctcattt gcagcctgct
     8641 tcagcctttt catatattcc gaaaactccg cttctttttc tccatatgtc aggttcagtt
     8701 catattccag cgggagccat gtcgatatgt ctgcctcatt gtgctggata atggcctcca
     8761 tcctgtcaag agcccgaaaa atcctggact cagtggtttc ctgcgcatac atttcttcga
     8821 acagtccgaa aagctcctcc ctgtacggct ccggcaaact gtcaagaaac tgctgcagtt
     8881 tctgtctttc atcgagttcg tcgctgtccg ttttgctgaa tgcaggtata tcacccgtga
     8941 aaacttctcc gatgtcgtgg aatatacaca tgaggatgac tttgtttata tcagcgtccg
     9001 ggaattcatc cttcaccagg tacgccatca tcgacagtct ccagctgtgt tccgccacgc
     9061 tctcctgacg gccggaagac gtccatgaat gccttgtatt gtttttcagt ttctccgcta
     9121 ctgagagtat ttcgatcagt tttcttacgt ccactttttt aatcctccca ccgattattt
     9181 ctctttcagc cgccctttgc tccacatttc ctaaagtatg aattcctcga tccactttgc
     9241 gacgccgtca tcatcgttgc tgccgcagat gtagtctgca gccctcttca cttcttcgac
     9301 ggcattcctt acagccacac cagtgccgca attccttacc atttccagat cgttcatgtc
     9361 gtcgccgaaa gcagcgatca tatccgtatc gatgccataa tatccggcaa cggccctgac
     9421 agcattccac tttgaaacgc tgcgaacacc aatgctatat aagtcttcgc cggtatgccg
     9481 taaaaagctg agcgcaggga aatctgaaat tatgcaaccc ccgcgttacc caagtctcat
     9541 gtaaatgctg cgctctttga atcccatcga cttccagaat tttatccccg tttcattcca
     9601 ccagagcact tcgatatcgg ccctgtcggc gcccagcagt ttcagcagtc ctgtcagggc
     9661 agcccgccca tagccgcgcc tgcggtactc ccttgaaatg aaaaactgcc tgatgtacaa
     9721 aggatccctg ctcatatcga ccagagcata accggctact ttgccgtcct tcaggaacag
     9781 gtaagcattg tagttcccgt cgatgaatcc cctcatcctg tcccttaatt ggcttacatc
     9841 cattttattg tcgtgctttt catcctctat cagttgcttg ttcatgcctg ccagtatgtc
     9901 caggtcatca tgtgtggcaa gctttaaaac aatatctccc acggtatcac tcccccccga
     9961 agtttttaaa gccggttccc tgcaacttcc ctgatctttt tcaaactcac cggatataga
    10021 ggccgagaac cagacgtttt ttctgctttc tcacttttat cgggataccg gcatgctcat
    10081 ttttcatata ttcgataaac gactcgcctg tgccttttgt aaatcccgac ctgtcaagac
    10141 gcagccccag ccttcttgcc atcaccagga tcaggtaatc cctgtcggcg atgaactctt
    10201 tgacctgggc ccactcgtat tcaatggcgg aattcccgtc agtcactttg attttgtcgc
    10261 ccagttctat tctgcgttcc cattcgtcct taccctgaac aatggtaagc gccttgaact
    10321 gccttgccag aagcagcctc ttcctgaaaa agatcataaa aacgcagaac agttcaagaa
    10381 aagcatacag caataagtag acgatatcgt cgtaagctgc agatattact gcaaatgcag
    10441 ggatgatgac cgccattgct atccacaagt attgagtgac atcaggtgtg accggccgtt
    10501 tggcccattc gagaaaacgc aattttgaca cagtatactc gttcacaaac tggggatcca
    10561 tttcatgtcc tccttccagt cctccgatgt tgcttcatac aggcaataat caccgctgat
    10621 cctttcgcag tcaggaacct ccctgtattt catcccgata aaacgcatgc cggacttctg
    10681 cattactctt ccgttcgcgg gattgtcagc atggtgtcgc gcatggactt tggcaaaccc
    10741 tattttattg aaagcgaaat ccaaaacagc cctgcacgcc tcagtcatta tacccctcgt
    10801 tttatcggtc aatccgccag gatatattct tcaagccatt tagcgacacc gtcattatcg
    10861 tttgtgtcac atatgtattt tgcagccgcc ttgacttctt cgacagcatt ccctactgca
    10921 acgccgatgc cgcaattctt tatcatctcg gtatcgttta catcatcgcc gaaagctgca
    10981 accgtatctg tatcaatatt aaaatacccg gcgacagctt tgaccgcatt ccactttact
    11041 gccctgcggt cggcaaacct gtacagatcc tcgcccgtat accgcagcaa atcaagagtt
    11101 gggaactccg aagctatacg ttcgacaaca tccggattcc ggctgatcaa tgagattttc
    11161 aggtaactta catcacttaa ctgcgaaaaa tccacacact cataatgaac aatttctgaa
    11221 ttcggcgaat ccaggctgtt ggcatgtgcg acagtttcat tacacgcatg tacatatggg
    11281 atctcaggct cccgcagaca tctgtctatc agtttggaac caatctcagc gggaatggcg
    11341 aaacgggaaa tcactttttc ccccgcaaat gaaacagcgc ctccatatcc aataaacaca
    11401 tcgggagcga accgctcaaa gaaccttgat accgtctgtt ttgacctcgc ggtcgcatat
    11461 gcggtcctga tccctctgtc ccggcatttc ttcagtacag atacagtata atcggaaatg
    11521 gttttgtcgc ttcgcagaag cgtatcatcc aaatccatca ctatcatctt cactacagtt
    11581 ttgaccccgt tagttttcat agatgttttc cgtatgtctc cttgtgtaaa attacaatag
    11641 atccgttttt atcgttgata cagtacagca tgattaaaat ctgaattcta acgactgttg
    11701 tcatcacagt cacatactta agccgatcct caagccttgc cctggcaccg gtgatatttt
    11761 accaggataa tgatactatc agtaaaacga ccgaaacgcc aaacagtatc cagtatgata
    11821 ctttgtgtcc atatgaccag aagtcgttgg gggttatgtc atctgaatcg gtatacatgc
    11881 ttatgcgaaa tttgttcact gcccagatca tattcgggta tccggcagag actcccacca
    11941 tgagagcaga taatgcagca aggatacccg ccgttggctg gcctgaaaat ataaggcaca
    12001 gcaacagttc tgccgccgcg agcgagaaaa aaatccactt cagtatcctc tcgaatcgtg
    12061 atgccgtcaa a
//
LOCUS       DAOSCC010000051        11892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3617_length_11892_cov_4.537805, whole genome shotgun sequence.
ACCESSION   DAOSCC010000051 DAOSCC010000000
VERSION     DAOSCC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_11892_cov_4.537805"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PLG72_04285"
     CDS             <1..1109
                     /locus_tag="PLG72_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ80050.1"
                     /translation="PDNVEKVSGIGPIRVDQVAIGSCTNSSYMDMIKVAKILKGKTVH
                     PNVSLVIAPGSKQVLTMLAKEGALADLVAAGARILESACGPCIGMGQAPATDAVSLRT
                     FNRNFEGRSGTKSAKVYLVSPETAAASAITGVLTDPRTLGEAPEVSMPESFYINDNMI
                     VPPAPEGEEVEVVRGPNIKPFPVNKELPDKVEGTVLIKVGDNITTDHIMPSNAKLLPF
                     RSNVPYLAEYCLTPCDPDFPKRAKENGGGFIVGGSNYGQGSSREHAALAPLQLGVRGV
                     LAKSFARIHMANLINSGILPMTFADEADYDRIKDGDVLVLENAREQIKAGDELTLVNK
                     TANTTIKVKIALSDRQKDIMLAGGLLNYTKKQGQ"
     gene            1251..1919
                     /locus_tag="PLG72_04290"
     CDS             1251..1919
                     /locus_tag="PLG72_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ80051.1"
                     /translation="MVKVDFGDTGQQSNSLHSRVYNQIRNDILNGVYEPGESLVEMKL
                     SEELGVSRTPVREALRQLELEGLVQSVPNRGVTVRGISQQDIQDIYTIRMMIEGLAAR
                     WAAEKITPEEMEELKEAVDLEEFYTVKHNYSNLLQFDTRFHDIIFKASKSMPLMHTLS
                     TFHSYVQKARKVSMSSPERAAAALEEHKAIMQAIIDRDADRAEKLMTEHVRNASLNLL
                     KHGE"
     gene            complement(2212..2286)
                     /locus_tag="PLG72_04295"
     tRNA            complement(2212..2286)
                     /locus_tag="PLG72_04295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2251..2253),aa:Glu,seq:ctc)
     gene            3012..4421
                     /gene="amrA"
                     /locus_tag="PLG72_04300"
     CDS             3012..4421
                     /gene="amrA"
                     /locus_tag="PLG72_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOJ80052.1"
                     /translation="MGVIKKAFISPHPPIVVHEVGKGEEKKAVATIEALERLAREVAK
                     IKPSTIIVTTPHGPVFQDYIFINHNDVLSGDFRRFGAAEVKLRYENNLKLAGEITRLA
                     NSQGIPCGGLDGNLIKRFRISEELDHGAMVPLYFINKLYGGFRIVKISIAAMPFKELY
                     RFGRCIAKAVEQSGEEAVFLASGDLSHRLAPDGPYGFNECGPEFDGRLVDYLKVPDRE
                     AILGFDEGFLEEAGECGLRSFIMMFGALDEFEIKPEVYSYEGPFGVGYAVAEFTPASR
                     IEGETLLDRLDRREIEEMSMIRSSEDPYTALARKSLEMYVLNGTIIDVPEVLPEEMRS
                     GRAGVFVSIKKHGRLRGCIGTIMPTRENIAAEIIHNAVSAGVHDPRFDPVTEDELDDL
                     VYSVDVLGDPEPVSSMDELDVKRYGVIVRAGRRSGLLLPDLEGIDTPQQQVAIALQKA
                     GIRPDEKYTMERFEVVRHR"
     gene            4557..5594
                     /locus_tag="PLG72_04305"
     CDS             4557..5594
                     /locus_tag="PLG72_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ80053.1"
                     /translation="MREAAYYEKLDNGSVRCMLCPRRCFLQQGKTGVCRARRNIGGKL
                     YSLNYGYIASVALDPIEKKPLYHFHPGSYILSAGTFGCNFKCRWCQNWSIAHGEDQPA
                     SRMQDGFTDQEDSRVPDSGKGQSAGSVNGGRQLSGHGNGPFAEEEEEQAHAWNHRDVM
                     LAELTPEKLASLAEKYLSRGNIGVAYTYNEPTVWYEFVLETAKLVREKGLVNVLVTNG
                     SINEEPLEELLPYIDAMNIDVKAFTVDFYGRYCKGSLEHVKRTVERAAQSCHVEVTTL
                     VIPGLNDSPWEIGELARWLSAIDRDIVLHLSRFFPNYKMLHIGPTPLETLERAKRSAQ
                     EHLRHVYLGNV"
     gene            5802..6626
                     /gene="cdaA"
                     /locus_tag="PLG72_04310"
     CDS             5802..6626
                     /gene="cdaA"
                     /locus_tag="PLG72_04310"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ80054.1"
                     /translation="MGFDNPLYIIRALLDIGIVTWVVYKLIILVRETRAWQLIKGLLL
                     IIIATEMSGFLELKTINYLLRSVLSVLAIGAIVLFQPELRRALEKIGRSRFKDLLNFD
                     EASSLRVQTTAVIEEIVKTCTFLSSRYIGALIVIERDVKIGEIINSGIELDSHVSSEL
                     LINIFTPDTPLHDGAVVIRNNKIKAAACLLPLTDNPSLSKELGMRHRSALGITEVSDS
                     IAIVVSEESGKISFALNGGLTRNLTADTLRKALNKNLLPEREANSKKLILWRGKTK"
     gene            6623..7918
                     /locus_tag="PLG72_04315"
     CDS             6623..7918
                     /locus_tag="PLG72_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOJ80055.1"
                     /translation="MRIADWFKKDIKIKVLAFFIALLFWLYVSNVTNPFKTVTIYNVP
                     VNEINKDYLDENDYKLKNQPRTFIDITVRGRQNVVEKIRSTDFEVYMDYSQIKSVSYK
                     KLTLSEPVCMFKNVTIESYNPKEVDIQLTRYKTKYFDLEVVSNVTMKPGYVMLRATAS
                     TEQIPVIQEETVVDSIASVKAFIELADVDRDTVVQQVQCKAFDKNGNEIPNIDLMKVN
                     VTIETAKEVPVSLVTRGRLAANHIEILDNRVVEPSRVLVKGPPETLENLKEIKTEQVD
                     IDGLDKDLSTTVPLVVPEGTELVNSPKDARVFMDIEGLVVRNFEFTKDDIAILNARND
                     GTLVYEIVTDKVLVQFRGLQTDLNSIVASSLRPAVDVADLAEGTHRLQLNLNLPQTGN
                     LNLVQRAYVEVRISRTPETPPEENQAGEQQSEEKPPANP"
     gene            8082..9431
                     /gene="glmM"
                     /locus_tag="PLG72_04320"
     CDS             8082..9431
                     /gene="glmM"
                     /locus_tag="PLG72_04320"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ80056.1"
                     /translation="MARLFGTDGVRGVANTELTAELVYKLGQAGAYVLTSETHHTPKI
                     LVGMDTRISGNMLESALVAGICSVGAEAVCLGVIPTPAVAYLTRYYKADAGVVISASH
                     NPFEFNGIKFFDSRGYKLPDATEDKIEELVRNGFDNSLLPSGAGVGRRKTAENTLRDY
                     VDFLKSTIDHDLKGLKIALDCANGASYQAAPMLLEELGAQVLPINNCPDGLNINRDCG
                     STHISRLKEYVKECGADVGLAFDGDADRVLAVDENGELVDGDKIMAIIGLELKKAGQL
                     AHDTIVVTVMSNIGLDIMARENGLNLVKTKVGDRYVLEEMLDKGYVLGGEQSGHIIFL
                     KHSTTGDGLLTALQLLNVMRSTGRRLSDLAGVMQVLPQVLKNAKVKNENKHRYMEDEL
                     VAKLCRELEDEFKGEGRVLIRPSGTEPLVRVMIEGKDREYIAKRASELVSVIEERLG"
     gene            complement(9610..10269)
                     /locus_tag="PLG72_04325"
     CDS             complement(9610..10269)
                     /locus_tag="PLG72_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOJ80057.1"
                     /translation="MDVRITDLFDLNECFEREIFDGLEYPWEALPRIKTFLLEFAKTL
                     PADFEQISENVWVGKGTTIEKTVTIKGPAIIGYNCELRQCAYIRENVIIGNECVIGNS
                     TELKNALLFNKVQVPHFNYVGDSILGYKAHTGAGVICSNLKSYGTNVKVTDGYRTIET
                     GLRKFGAMIGDNSEVGCNSVLNPGTVIGKNCIVYPLTSVRGYVPSNHVLKSSGVMVIR
                     T"
     gene            10481..11413
                     /locus_tag="PLG72_04330"
     CDS             10481..11413
                     /locus_tag="PLG72_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80058.1"
                     /translation="MGDCVFDKARKWICRNARPLDRERFRYLFENGSREAVLSALAEY
                     QNEDGGLGHALEADCFNPNSSPIQTWAATEILHEIGLEEDHPIVRGILAYLDSGRDFN
                     GKCWLYTVPSNNDYPRAPWWTYDSITYDTVTYNPTAALAGFALIFAPRESGLYRKCYE
                     IAKEAIRYLEETDKCDEMHTLACYVRLAECCRKAGLADELGIGELEEVLVQRINKVMT
                     WDTESWKTKYVCKPSNFFMDKDSYLYRHFAELAERECEFIRNAQQPDGTWSINWNWEG
                     HEEQWHIAKNWWKAHLAIMNLRFLQGMTGEPAGR"
     gene            complement(11447..>11892)
                     /locus_tag="PLG72_04335"
     CDS             complement(11447..>11892)
                     /locus_tag="PLG72_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOJ80059.1"
                     /translation="LQISNVFISNADMRKLFKQELERTGYSQPNIMGLVACRAAYKYG
                     AQWLDDLIRYLVSNLDLLRDFLRHELPSIRLVEPEGTYLVWLDFRALGIPDKELNDLI
                     VHKAGLWLNAGTMFGAGGEGFQRMNIGCPAAILREALERLKKAFR"
BASE COUNT         3289 a         2738 c         3267 g         2598 t
ORIGIN      
        1 gcccggacaa cgtcgagaag gtttccggca tcgggccgat cagggtcgac caggtggcta
       61 tcggaagctg tacgaactca tcctacatgg atatgataaa ggtcgcaaag atactgaaag
      121 ggaagaccgt acatccgaac gtgagccttg ttatagctcc cggatcgaag caggtgctga
      181 ccatgctggc aaaggaaggg gcattggccg accttgtcgc agcaggcgca aggattctgg
      241 aatcggcatg cggaccgtgc ataggcatgg ggcaggctcc ggcaacggat gctgtctctc
      301 tgaggacgtt caacaggaac tttgaaggaa ggagcggcac aaagtcggca aaggtatacc
      361 ttgtcagccc ggaaacagcg gcggcaagcg ccattaccgg cgtactcacc gatccgcgca
      421 ctttgggcga agccccggaa gtttcaatgc cggaaagctt ttatataaac gacaatatga
      481 tcgttccgcc ggcgcccgaa ggggaagagg tcgaagtggt aagaggcccc aacataaagc
      541 cgttcccggt gaacaaagag cttcctgaca aagtggaggg cactgtgctc ataaaggtgg
      601 gggacaatat aaccaccgac catatcatgc catccaatgc aaaacttctg ccgttcagat
      661 ccaatgtgcc ttacctggct gaatactgcc tgactccgtg cgatcccgac ttcccgaaga
      721 gggctaagga aaacggaggc ggttttatcg taggcggatc gaactacggg cagggctcga
      781 gccgcgagca tgccgctttg gctccgctgc agctgggtgt caggggcgtg ctggccaaat
      841 cctttgcaag gatccatatg gcaaacctca taaactcagg catactgccc atgacatttg
      901 ccgacgaggc tgactacgac aggatcaaag acggcgacgt gcttgtgctc gagaatgcaa
      961 gggagcagat aaaagcagga gatgagctta cgttggtaaa caagactgcc aacacgacga
     1021 taaaggtgaa gatcgcattg tctgacaggc agaaggatat catgctggcg ggcggattgc
     1081 tgaattatac gaaaaagcag ggacagtagc agcagggtcg acagaaggag ttatataaat
     1141 cccaggattt gtttgcggta aaattcacgg caacgtaata agaatgcatg cggtacgaat
     1201 gggatatcgg gcataacatg ctgcggatgc agcagtatta gggagtgaac attgtgaaag
     1261 ttgatttcgg tgataccggt cagcaatcaa attccctgca cagcagagtt tacaaccaga
     1321 tcaggaacga catcctgaac ggcgtatatg aacccggaga gagcctggta gaaatgaagc
     1381 tgtcagagga acttggcgta agccggactc cggtgcgaga agcactcagg cagttggaac
     1441 ttgaaggact ggtacagtcc gtaccaaaca ggggtgtcac tgtcaggggc atttctcagc
     1501 aggacataca ggatatttat actatcagga tgatgataga agggcttgcg gcaaggtggg
     1561 ccgcggaaaa gatcacgccc gaggagatgg aggaactgaa ggaagcggtc gacctggagg
     1621 agttttatac tgtgaaacac aactacagca acctgctgca gttcgacaca cggttccatg
     1681 acataatttt caaggccagc aagagtatgc ccctgatgca tacgctgagc acgttccaca
     1741 gttacgtcca gaaagccagg aaagtatcga tgagcagccc ggagagggca gcggcggcgc
     1801 tggaggaaca caaagcgatc atgcaggcta tcatagacag ggacgccgac agggcggaga
     1861 agcttatgac agaacatgtg aggaatgcga gcctcaacct tctaaagcat ggagaataat
     1921 aagggcaggg gacacttctg caaaatccat ttgcagaaat gtccctttgt ttgaatccct
     1981 tttagcctga aataaaaaca cctcggtttg accgaggtgt ttttatggtg acccataggg
     2041 gattcgaccg cccagcttcg ctgtgctgac cgcacggctg ttaagctccc cggtaagccg
     2101 tgcgcattcg ctaaaaaatg cggtgcattt ttcttaacgc tcatgccctc ccgggttcga
     2161 atccttttta gcttataata aaaacacctc ggtttgaccg aggtgttttt ctggtgaccc
     2221 ataggggatt cgaacccctg ttaccgccgt gagagggcgg tgtcttaacc gcttgaccaa
     2281 tgggccgtgg tgtttgattt cacatgcgca tgtgttatta taacatgaca ttttatcaaa
     2341 tacaataggg aaatgatctg gatcgcctgt tggaatttcg tgggttttgc tttgcagata
     2401 atgtttcaga tatacatgcg agaaatgctt tttcaacttg gctattttgc tctgattttt
     2461 cagtgtttta tgtcacctgt ttattgaata ttttcaatga aaataacggg taattaacgc
     2521 agaagttgaa attttgagca acggggccaa caaaatttaa acaacagaaa tttcatttta
     2581 cctggaagga taaacaggac gtgttgttga atcaattata atagcatcgc agctggacct
     2641 gccgaagggt ttgacagggt cgatcagtaa cgcaacaacg aggttgaatt tacatggata
     2701 aaaaatttat cagatggata gcaataataa tcacactggg ttttttactg acatctttca
     2761 gttttatcgc atatatgctg ctgttcagca actgaagtca acagctgtcc ggcaactgga
     2821 gaaaacaggc taagagatgg acaggcagcg ggattattga tgtggaacaa tgggataatg
     2881 agacagagac agtgaaatag tgaaatagca aaatgtgaga taatgggata cagccggcaa
     2941 aagaagaaaa aggcgaaata gtcggcgact gatcatgaga ggcataataa cagcggaaag
     3001 gtcgtggaca tatgggagtg ataaagaaag cgttcatctc gccgcatccg ccgatcgtag
     3061 tacacgaggt gggaaagggc gaagaaaaaa aggctgtcgc tactatagaa gcacttgaaa
     3121 ggcttgcacg ggaggtagcg aagataaagc cttcgacgat cattgtcacc accccgcatg
     3181 ggcccgtatt ccaggattac attttcatca accacaatga cgttctgtcg ggcgatttca
     3241 ggaggtttgg cgctgctgaa gtgaagctgc gttatgaaaa caacctgaaa ctggccgggg
     3301 aaataaccag gctggctaac agccagggca taccctgcgg cggtctcgac ggcaacctga
     3361 tcaaaagatt ccggatatcc gaggaactgg accatggagc aatggtgcct ctgtacttta
     3421 tcaacaagtt atacggcggt ttcaggatag tcaagatctc aatagcggca atgcccttca
     3481 aggaactgta caggtttggc cggtgcattg ccaaagcggt cgaacagtcg ggtgaagagg
     3541 cggtattcct ggcgagcggc gacctgtccc acaggctcgc gcctgacggc ccttacgggt
     3601 tcaacgaatg cggccctgaa ttcgacggca ggctggtgga ttatctgaaa gtgcccgacc
     3661 gtgaagcgat actgggattc gacgagggat tccttgaaga agccggggaa tgcggacttc
     3721 gttcctttat aatgatgttc ggcgccctgg atgaattcga gatcaaacct gaagtatatt
     3781 catacgaagg tcctttcggc gtaggctatg ccgttgcgga attcactccg gcgtcaagga
     3841 ttgagggaga gaccctgctt gacaggctgg acaggagaga gatagaagag atgagcatga
     3901 tacggagttc tgaggacccg tataccgctc ttgccaggaa gtcactcgag atgtatgtcc
     3961 tgaacggcac cataatagac gttccggaag tgctgccgga agaaatgcgg tcaggacggg
     4021 cgggagtctt tgtatcgatc aagaaacacg gaaggctgag gggctgtatc gggaccataa
     4081 tgccgacaag agagaatatc gccgcagaaa taatacataa tgccgtaagc gcaggcgtcc
     4141 atgatccgag gttcgatcct gtcacggagg acgaactgga tgacctggta tactcggtgg
     4201 acgtgcttgg cgatccggaa ccggtcagtt ccatggacga actggatgtc aaaaggtacg
     4261 gcgtcatcgt aagagcgggc aggcgttcgg gactgctgct tcccgacctg gaaggcatag
     4321 acacccctca gcagcaggtc gcaatagccc tgcagaaagc aggtatccgg cccgatgaaa
     4381 aatatacgat ggaacgcttt gaagtagtga ggcacaggta attgggagaa tcaagcaagc
     4441 aatttctaat gatactgcag aggtgatctg cagtaatacg gcgaatgtta tttgcagtga
     4501 aaccataaat acatcacata cgaatgagag ataaaacact aatgacgggg tgggtcatga
     4561 gggaagctgc atattacgag aaactggata acggcagcgt ccgctgcatg ctctgtccgc
     4621 gccggtgctt cctgcagcag ggcaaaacgg gcgtgtgccg tgcccgcagg aatatcggcg
     4681 gcaaactgta ttcgctgaat tacgggtaca tcgcttcggt ggcgctcgat ccgatagaga
     4741 aaaagccgtt gtaccacttc catcccggaa gttacatact gtcggcaggc acattcggat
     4801 gcaatttcaa atgcagatgg tgccagaact ggtctattgc ccatggggaa gaccagccgg
     4861 cttcacgcat gcaagatggt tttacggatc aggaagacag tagggttccg gacagtggaa
     4921 aggggcaatc ggccggcagc gttaatggag gcagacaatt gtccggacat ggaaacggcc
     4981 cgtttgccga agaggaagaa gagcaagccc acgcctggaa ccacagggac gtcatgctgg
     5041 ccgaacttac gcctgaaaag ctggcaagcc tggctgagaa atatcttagc cggggcaata
     5101 taggtgttgc atatacatat aatgaaccga ctgtatggta cgagttcgta ctggaaactg
     5161 caaagctcgt cagggaaaaa ggcctggtga acgtactggt caccaacgga tccatcaatg
     5221 aagagcctct cgaagaactc cttccgtata tcgacgcaat gaacatagac gtaaaagcgt
     5281 tcaccgtgga cttctacggc agatactgca agggaagcct ggaacatgtc aaaaggaccg
     5341 tggaaagagc ggcgcaaagc tgccatgtgg aggtcacgac actcgtgata cccggcctga
     5401 acgattcacc gtgggaaata ggggaacttg cacgctggct ttccgccatc gacagggata
     5461 tcgtactgca tctgtcgcgc ttcttcccga actacaaaat gctccacatc gggcctacgc
     5521 ccctggaaac tcttgaaagg gcaaaaagaa gcgcccagga gcatctgagg catgtttatc
     5581 tcggcaatgt ctgacatagg cttatttgtt ttgtataatg catacaactc ttccatcaaa
     5641 tattttatat atttgttgaa aacttcttaa caaatataag ataaaaaagg tataattcat
     5701 atgagagata caacagattt acacaaacag gggtgtggat atttttggac ctgttcagca
     5761 acataaacct gcgcgacctg ttcaataatt tcgtcggcta catgggattt gacaatcctt
     5821 tgtatataat ccgcgcctta ttggacatcg gcatcgtaac atgggtcgta tacaagctca
     5881 tcatactggt ccgcgaaaca cgtgcctggc agttgataaa aggtctcctt ctcatcataa
     5941 ttgcgactga gatgagcggc ttcctcgaac ttaaaacgat caactacctg ctcaggtcgg
     6001 tgctgtcggt attggcaata ggcgccatcg tccttttcca gcccgaactg aggagggctc
     6061 ttgagaaaat aggcaggagc aggttcaagg atctgctgaa cttcgacgag gcgtccagcc
     6121 tcagggtcca gacgacggca gtcatcgagg aaatagtaaa aacgtgtact ttcctttcat
     6181 ccaggtacat aggcgccctt atcgtaatag agagagacgt gaagatcggc gagatcatca
     6241 acagcgggat agagcttgat tctcatgtat caagcgagct gctcatcaat attttcaccc
     6301 ccgatactcc gctgcatgac ggagcggtgg taatacgcaa caacaagata aaagccgcag
     6361 cctgcctgct gccgctgacc gacaacccga gcctgagcaa ggagcttggc atgaggcaca
     6421 ggtcggcgct ggggatcacg gaggtatcgg acagtatcgc gatagtcgtt tccgaagaat
     6481 ccggaaagat atcctttgcc cttaacggcg ggcttacaag gaacctcacc gctgatacgc
     6541 tgaggaaagc cctgaacaag aacctgctcc cggagcgtga ggcaaattcc aaaaaactta
     6601 tactgtggag gggcaaaacc aagtgagaat cgcagactgg ttcaagaaag acataaaaat
     6661 aaaggtcctg gcctttttta tagcactgct gttctggctt tatgtttcga acgtgaccaa
     6721 ccctttcaaa acagtgacta tatacaatgt gccagtgaat gaaataaaca aggattatct
     6781 tgacgaaaac gattataaac tgaaaaacca gcccaggaca tttatagaca taaccgtacg
     6841 gggacgccag aatgtggtgg aaaagatcag atcgacagat tttgaggtct atatggacta
     6901 ctcgcagatc aaatcggtca gctacaaaaa gctcacgctt tcagaaccgg tatgcatgtt
     6961 caagaacgtg acgattgagt catataatcc caaagaagtc gatatccagc ttaccaggta
     7021 caaaacaaaa tatttcgatt tggaagtcgt atccaacgta acgatgaaac cgggttatgt
     7081 gatgctgcgc gccacggctt cgaccgagca gataccggtg atccaggaag aaacggtcgt
     7141 cgacagcatc gcctccgtaa aagctttcat cgaactggcc gatgttgaca gggacaccgt
     7201 tgtacagcag gtacagtgta aagcattcga taagaacggc aatgaaatac ccaatatcga
     7261 tctgatgaag gtcaatgtca ctatagagac ggccaaggaa gtgcccgttt cccttgttac
     7321 aaggggaagg cttgccgcca accacatcga gatcctggac aacagggtgg tagagccttc
     7381 cagggtactg gtgaaaggcc ctcccgaaac gcttgaaaac ctgaaggaga tcaagacgga
     7441 gcaggtcgac atcgacgggc tggataagga cctcagcacc acggtcccgc tggtcgtccc
     7501 tgaaggaaca gaacttgtga actcgccaaa agatgccagg gttttcatgg atatagaggg
     7561 acttgtcgtg aggaattttg aattcacgaa agacgatata gcgatactga atgcgaggaa
     7621 tgacgggacg cttgtatatg agatcgtcac agacaaggta ctggttcagt tcagaggatt
     7681 gcagacagac ctgaacagta tcgttgcatc atcgctgagg cctgcggtgg atgtcgccga
     7741 tctggcggaa ggcacccaca ggctgcagtt gaacctgaac ctgccccaga caggcaacct
     7801 gaacctggtg caaagggcct atgttgaggt ccggatatcc cggactccgg aaacgccgcc
     7861 ggaagagaac caggcaggcg aacagcagtc agaagagaaa cctccggcga acccataaat
     7921 cacaggaaca gctccatttt gactgatacg tccatcctgt gcacgacgat acaataaagt
     7981 gcaaaaatgc aaaaaaattc atgctaaagc attgaaatac aggtatgaat tgcattatca
     8041 tattggtgtt atattaaaat ctattcacca gggggaaaaa tatggcaagg ctttttggaa
     8101 cagatggcgt caggggagta gcgaacacgg agcttacagc ggagcttgta tataagctgg
     8161 ggcaggcggg agcgtatgtg ctcacttccg aaacgcatca cactccgaag atattggtgg
     8221 gaatggatac gaggatatcc ggaaacatgc ttgaatccgc ccttgtggca ggtatctgct
     8281 cagtcggggc ggaggcggtc tgcctgggtg tcataccgac acctgcagtg gcatatctta
     8341 cgagatacta caaagctgat gccggcgtag ttatttcagc ttcccacaat ccgttcgaat
     8401 tcaacggcat caagtttttc gactcgaggg gatacaagct gccggatgct acggaagata
     8461 agatcgaaga actggtcaga aacgggttcg acaattcgtt gctcccgtca ggcgccgggg
     8521 taggcaggag gaagacagcc gaaaacaccc tgcgcgacta tgtcgatttc ctgaagagca
     8581 ccatagatca cgatctgaag ggactgaaaa tagcgttgga ctgtgccaac ggcgcttcat
     8641 accaggcagc cccgatgctg ctggaagaac tgggagccca ggtcttaccg ataaacaact
     8701 gccctgacgg tctgaacata aaccgtgact gcggttcgac ccacatatcg cggctaaagg
     8761 aatacgtcaa agagtgcggc gccgatgtcg gccttgcatt tgacggcgat gcggacaggg
     8821 tgcttgccgt ggacgaaaac ggcgaactgg tggacggcga caagataatg gcgataatcg
     8881 ggctggaact gaaaaaagcg ggacagcttg cccatgatac gatagtggtc acggtgatga
     8941 gcaatatcgg actggacata atggcacgcg aaaacggcct caacctggtc aagaccaaag
     9001 tgggcgacag gtatgtgctc gaggagatgc tggacaaggg atatgtactg ggcggcgagc
     9061 aatcaggaca cataatcttc ctgaagcaca gcaccaccgg tgacggcctg cttactgcac
     9121 tgcagcttct gaacgtgatg aggtcgaccg gccgcaggct gtctgacctg gcaggcgtga
     9181 tgcaggtgct gccgcaggtt ttgaagaatg caaaagtcaa aaatgaaaac aagcacaggt
     9241 acatggaaga cgaactggtc gcgaaactct gccgtgaact ggaagatgag ttcaagggcg
     9301 aaggcagggt gctgatcagg ccttcgggta cggaaccgct cgtgcgtgtc atgatcgaag
     9361 gaaaggacag ggagtatata gcaaaaagag cttcggaact ggtctcagtg atcgaagaaa
     9421 ggctcggctg atcctggaga agagcggccg ggatgatatg gcgcaggtat agtatgacta
     9481 aaaaagcctc ggctgaggat acggacctca actgaggctt ttttgcatgt actgtttcgt
     9541 tttcggtatg gcttcgccac gacgtatcct gcagcctgaa tgagcagccg cttatgtatg
     9601 catagccgct taagtcctga tcaccattac accgctgctc ttcagtacat gatttgaagg
     9661 cacataaccc ctcacagagg tcaggggata aactatgcag ttctttccga tgaccgttcc
     9721 cggattcagc acgctgttgc agcctacttc cgagttgtcg ccgatcatgg cgccaaattt
     9781 ccgcaggccc gtttctatag tcctgtaccc gtcggtcact ttgacattcg tgccgtagga
     9841 tttcaggttc gaacagatga cgcctgcccc ggtatgtgcc ttatatccaa ggatcgaatc
     9901 tcctacatag ttgaaatgcg gtacctggac cttattgaac agcagagcgt tcttcagctc
     9961 cgtggagttg ccgatgacac actcgttgcc tatgatcacg ttttccctta tgtacgcaca
    10021 ttgcctgagt tcgcagttat acccgatgat agccggcccc tttatcgtta ctgtcttttc
    10081 gatagtcgtg cccttgccga cccatacatt ttcgcttatc tgctcgaaat cggcaggcag
    10141 tgttttcgca aactccaaaa ggaaggtttt tatcctcggc agcgcttccc acggatattc
    10201 caggccgtcg aaaatctctc tttcaaagca ttcattcaga tcaaacaggt cggtgatcct
    10261 aacatccata cattttcctc tttctataaa aagtttgccc gtaactgccg atgcagcctg
    10321 ccattcttta tatatgctcc ggggtgcagt ataatattgc tgcgggtaac tttattttaa
    10381 cataaactgc atttttgggt acactatccg ggaaaaaaga caggacgatt ttccgatatc
    10441 tctgtacgtt atatataaaa agcataaagg ggaaatcagg atgggagact gtgttttcga
    10501 taaagcaagg aaatggatct gccggaatgc caggccgctg gaccgtgagc ggttcaggta
    10561 tctgttcgaa aacggaagca gggaggcagt attgtcagcg ctggcggagt accagaatga
    10621 agacggcggt ttagggcatg ccctggaagc ggactgcttt aatccgaact cctccccgat
    10681 ccagacctgg gcagcgacgg agatcctgca tgaaatcggg cttgaagagg atcatccgat
    10741 agtacggggg atcctggcat acctggacag cggcagggat ttcaacggaa aatgctggct
    10801 atataccgtg ccttcaaaca atgactaccc ccgtgcgcca tggtggactt atgacagcat
    10861 tacctatgac actgtcactt acaatccgac cgccgcgctg gcaggctttg cgctgatttt
    10921 tgcgccaagg gaaagcggcc tgtacagaaa atgttacgaa attgcgaagg aagcgatacg
    10981 ttacctggag gaaacggaca agtgcgatga aatgcataca cttgcctgtt atgtgaggct
    11041 ggccgagtgc tgccgcaaag cagggcttgc tgatgaactg ggcatcggag aacttgagga
    11101 ggtactggtg cagaggataa acaaggtaat gacatgggac acggagtcat ggaaaacgaa
    11161 atatgtgtgc aagccttcga acttctttat ggacaaggat agctacctgt atcggcactt
    11221 tgcggaactg gcggaacgtg aatgcgaatt tataaggaac gcgcagcagc cggacggtac
    11281 ctggtccata aactggaact gggagggcca tgaagagcaa tggcatatcg caaagaactg
    11341 gtggaaggcc catctggcca taatgaacct gaggttcctg cagggcatga ccggagagcc
    11401 ggccggccga tagtaaaaag gtttgttttt taccggatat gaactgtcag cggaaagctt
    11461 ttttgagcct ctcgagagct tcccggagga tagccgcagg gcagccgatg ttcatgcgct
    11521 ggaagccttc cccgcccgct ccgaacatgg ttccggcatt cagccacagg cctgccttat
    11581 gcacgatcag atcattaagt tccttgtccg ggatgcccag tgcgcggaag tcgagccaga
    11641 ccaggtatgt gccctccggt tctacaagcc ggatggacgg aagctcatgc ctgaggaagt
    11701 cccggagaag gtccaggttc gagacaaggt agcgtataag gtcatccagc cattgcgccc
    11761 catatttata tgcagccctg catgccacaa gccccattat gttgggctgg ctgtaaccgg
    11821 tcctttcgag ttcctgtttg aaaagcttgc gcatgtctgc atttgagata aagacattcg
    11881 aaatctgcag gc
//
LOCUS       DAOSCC010000052        11830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3642_length_11830_cov_4.825902, whole genome shotgun sequence.
ACCESSION   DAOSCC010000052 DAOSCC010000000
VERSION     DAOSCC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3642_length_11830_cov_4.825902"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLG72_04340"
     CDS             complement(<1..403)
                     /locus_tag="PLG72_04340"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOJ80060.1"
                     /translation="MDVIIKGDYEGLSKYAAKVIAGFVRKKPDCVLGLATGSTPIGTY
                     RELIRMHKEEGLDFSGVRTFNLDEYLGIGIDLSKPYNEDQSYARFMYEELFRHVNIKQ
                     ENVHIPDGRAKDPEKFCKWYEEEIKRAGGIDL"
     gene            793..2595
                     /gene="pepF"
                     /locus_tag="PLG72_04345"
     CDS             793..2595
                     /gene="pepF"
                     /locus_tag="PLG72_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOJ80061.1"
                     /translation="MTTKKLPARDEIDPKYKWRLEDIYSSEDDWEADFKKVQQLSGKI
                     SSYKGRLAGSMEALLECLELRDELSALTEKVFAYARMRRDENNAEPKYQALTDRAVML
                     MTEVNASTSFIVPEIISIDEELLRKSIDEMEGLSLYRHFFDDILRQKKHVLSDKEEEL
                     LALADEVMDSPSEIFSMFNDADIKFGTIRDENGEEVEVTKGRYIKFLESKDRRVRKDA
                     FHTLYDSYRAMRNTLAASLGSNIKRDKFFANVRRYGSSLQAALDSDNVPVDVYDNLID
                     TVNRNLPLLHRYVRLRKKALKLDELHMYDLYVPIVEQPTRHISYEEALETVKNGLAAL
                     GCCYVEDMTKAFGSGWIDVYENQGKTSGAYSWGTYLTHPYILLNYQGTINDVFTIAHE
                     MGHAMHSYYTNKTQPYIYSDYRIFVAEVASTVNESLLMDYLLSVTTDKNERAYLMNHY
                     LEEFRGTLFRQTMFAEFEKITHEMIEKGEALNADGFCKIYRGLNEKYFGAEVVIDDDI
                     DMEWARIPHFYRSFYVYKYATGISAAVSLSRQIIGDGVPAIDRYLEFLKSGGSDYPLE
                     LLKKAGVDLTVPKPVEDALKTFEKVLNDLEEIIG"
     gene            2666..3781
                     /locus_tag="PLG72_04350"
     CDS             2666..3781
                     /locus_tag="PLG72_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ80062.1"
                     /translation="MTDPRIETLAKNLVNYSCRVKPGEKVLIETKGFELPLTKALVKE
                     VYKAGGLPFVTIKNDEILRALLIGCSEEQIRLMARFELDRMKEMDAYIGIRYADNSNE
                     LGDVPEDKMKLYSTLFNKPVHSEQRVEHTKWVILRYPNSAMAQMAEMSTEAFEDFYFR
                     VCNLDYGKMSRAMDALVELMNRTDRVRIVGKGTDLTFSIRGIPAIKCDGHRNIPDGEV
                     YTAPVRDSVNGKITYNTPSKYQGFTYENVTLEFRDGKIINAAANDTERINKVLDTDEG
                     SRYVGEFSLGFNPYIEKPMKDTLFDEKIKGSFHFTPGSCYDICSNGNKSAIHWDLVCI
                     QRPEYGGGEIWFDDVLIRKDGVFVPKELEALNPENLI"
     gene            3903..5129
                     /locus_tag="PLG72_04355"
     CDS             3903..5129
                     /locus_tag="PLG72_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ80063.1"
                     /translation="MENWMIGTRFIILFYCVLRYFRSSMENTALVVLFMLLYISAVTT
                     VYLLRKTSLKTLFRMLSVTILAVSSVLATELFLLLMSADIYEIASSYIDDWRFLPVFA
                     AVPALLSIDTGVVLEYVLASLMSLLIFLIAQKLIQNVGALKEANEELRDSIDDLSVRL
                     SAGTEYEAQLRYLSQIEERNTLAQKIHDEVGHTLAGSILQLEAVGLIMEKDAAKAGEM
                     VRNVTENLKKGMESIRSTLRAIKPASEQLGINRLKLILEEFTLNNRIETEFSYEGRLD
                     AITHMQWSILTDNMREALTNSLKHSSASAVRVKIEVMNKVIKMEVKDNGRGEAAIKKG
                     MGIAGMEERTGAAGGKLIIDGSDGFSVITLLPVENDPERRIAAGRTGKQQPGEQHISR
                     TVDQSGGAYADQGIDS"
     gene            5104..5745
                     /locus_tag="PLG72_04360"
     CDS             5104..5745
                     /locus_tag="PLG72_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ80064.1"
                     /translation="MPIRVLIADDDALIREGLKIILQSDDRFEVAECVDNGCKAVEYC
                     STNQVDVALLDVRMPVMDGLEAAREISSKTGTKTLILTTFDDDDFIINAVKYGARGYL
                     LKNSPPARIMDAIRIVHDGGTVMQDVAMDIIKDELSSGRRCGIPDGILTEREMEIAML
                     VAKGLSNKDIAARLFMSEGTVKNYISAILAKTGLEHRTQLAVYYLTGEKGNGA"
     gene            5943..6881
                     /locus_tag="PLG72_04365"
     CDS             5943..6881
                     /locus_tag="PLG72_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80065.1"
                     /translation="MTIVKMEQVTKKFGDVTAVDKVSLEIEEGEIFGLLGPNGAGKST
                     AINMITGLLTIDRGSISIFGKDVRKDKMTTKSYVGIVPQDIAIYEDLTSLENVKFFAS
                     LYGLRGKMLEEAALEALEFTGLSEKKNQFPKSFSGGMKRRLNIACAIAHKPKLIIMDE
                     PTVGIDPQSRNHILQSVKKLNELGSTIIYTSHYMEEVEEICTRIAIMDHGKVIALGTC
                     PELENMINDRGVVVVTINEPASKVDENSLREIPGVENVDIGENTVKITSAKEVTNLDK
                     IIQYFIKNSFTIKNVESKTPDLETVFLSLTGRKLRD"
     gene            6898..8049
                     /locus_tag="PLG72_04370"
     CDS             6898..8049
                     /locus_tag="PLG72_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80066.1"
                     /translation="MIILNIIYKELKQCLRDFKTNILMVIFPIVLIVILGAAFSGVFE
                     NSIDIEDVNVLYTIDIEGGDPVFENAFSTFCEYMSDETGMQFEETADVEKGISEIENH
                     RYTAYLHITGDPPRIDMYRNKNSGFESGIVENAVASFLESYAAINVIVRNDPAALSKV
                     HQPDSAANSGYVRVTAIDRKKQPGSTDYYAVTMLTLILMYSSLTGFWSIRSEADAKTG
                     ARVLCAPVQKYQYLTGKILGSISVTIVQAFVVLLFSKLVLKANWGEDLLTVAVLIITY
                     AIMTVSLGAALAFIFRTAEVASGLMNTIIPVFVFLGGGYVPLAVLGDQISKISAISPV
                     RWINMALINIIYEGSYSDAYISLAVNLGLSILFISAAAWLSGRRKNAYA"
     gene            8042..9187
                     /locus_tag="PLG72_04375"
     CDS             8042..9187
                     /locus_tag="PLG72_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80067.1"
                     /translation="MRNLGLLILNNLKVTFRKKGNIITYIFLPVVGVLFSLVIHSTSS
                     VSILNLGFTDHDRGMAALELKEKLAATEDFSVIDVDEEEISKKLLDFELDAVVVVPEG
                     YTESILSGNAAEIEIVSLKGKETTAWVEQLIYRHSASMSLLSAASGGDPATFDRMLSQ
                     VSDNKVEFNVINAEDRSTDKAMTRTSIGFLVMFVMLGSGFTSMIVLKEKRDRTYHRIC
                     SAPVSSRQYIYANSLTGLIICILQIVMIQLIMKFVFRIDAGMSDFHLFIILLMFALVA
                     VGLGLLITAFSSSSYMAGTLNTLVLTPTCMLGGCYWNPELMPRFMQKIGYFTPQMWVM
                     EAIRKMQDGGALRSITLNLLVLAAFALAFMLIAAFRLSRTDNLQKFV"
     gene            complement(9435..11087)
                     /locus_tag="PLG72_04380"
     CDS             complement(9435..11087)
                     /locus_tag="PLG72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOJ80068.1"
                     /translation="MLSVKTVSAAQSNYEKAIDLKILGLLANKPADFELDRAPNRAEG
                     LAMLLRLLGKESEALKGGCKHPFKDVPAWADSYVGYAYQNGITNGKSKDTFGAHDPLT
                     AEQYVTLVLRSIGYRDGEDFQYNKALDKAVSLGILSNAEAKDLKNRSEFLRNDLVAVS
                     YNALTAKLKGSSRMLIEKLADTDRTVFKPAAVLLGLYPPDFEKRYGNVLSFSPRRTNY
                     GYVVRNADDLVRIITKSFVSHTVSFELDITGYNGDISEEFRSAVDIARDAAAVISGVE
                     NFMESWKYECNSHTMKVTVTYRETADEFTLSRSKVKAAFNKARYIVAERISRDMTDFE
                     REKVLHDYLVSNTRYDYINYKRNTVPEDSFNEYGCLVLGKAVCSGYAKAMKLLCDLAG
                     IDCMIATGWSTDSSREGHSWNIVKIDGEYYHLDVTNDDAITDDGTEVLTYTYFNLTDK
                     EMKKFYSWNTSLFPRCTSTRNNYYRKYGLVADNSEAMKRVLAEAIKNRSTVIEIKVSD
                     YSRYSYSDLRELIIKSGAIMRYMYTVNAELGIIKLLDIEYFE"
     gene            complement(11620..11760)
                     /locus_tag="PLG72_04385"
     CDS             complement(11620..11760)
                     /locus_tag="PLG72_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80069.1"
                     /translation="MADNERGGLFGCGFGFNWIWIIIIILVIILLFPCLFGRENEGYY
                     KE"
BASE COUNT         3168 a         2801 c         3059 g         2802 t
ORIGIN      
        1 gaagatctat acctccggct ctctttatct cctcttcata ccatttgcag aacttttccg
       61 gatctttggc cctgccgtcg gggatgtgga cattttcctg ttttatgttt acatgtctga
      121 aaagttcctc gtacatgaat cttgcatagc tctggtcctc gttgtacggc ttcgaaaggt
      181 cgatgccgat gccgaggtac tcgtcaaggt tgaaggtcct gacccctgaa aagtccaaac
      241 cctcttcttt gtgcatcctg atcagttccc tgtacgttcc tatcggtgta cttcccgtag
      301 ccagcccaag cacgcagtcc ggcttcttcc tgacaaaacc ggcaatcact tttgccgcat
      361 acttgcttaa accttcataa tcgcctttga ttatgacatc catattaagg tcccccttca
      421 accctgatcc agtttttcag catatccggg cattattccg atacttcacc attgaacggc
      481 ctgctgtacg aggcaaacag ctccaaacaa cacccggcct ttataccgga acgctaagca
      541 ttctgctaat tgcccgcgct ataatctata atatttttcg catttctcca tgtcaagaaa
      601 aaatattgac aatcggatac ttccgaagca ataaaatgga cagcataaat actggtaata
      661 aaacctgtca aagggtataa tgttatggaa aagatgcatt ggccggactg tatatctgcc
      721 gcgccatgtc ccggaccgcg gggcatgctt ccggcggtgg atgcgtccga atgttgagag
      781 aggagtggct ttgtgacaac taaaaaatta ccggctagag atgagatcga tcccaaatac
      841 aagtggaggc tcgaagacat atactcaagt gaggacgact gggaggcaga tttcaaaaaa
      901 gtccagcaac tgtcaggtaa aatctcctcc tataaaggca gactcgccgg cagcatggaa
      961 gccctgctgg aatgcctgga actcagggat gagctgtccg ctctgaccga aaaagtcttc
     1021 gcatatgcaa ggatgaggcg tgacgaaaac aacgccgaac cgaagtacca ggcgctgacc
     1081 gacagggccg ttatgctgat gaccgaggtg aacgcctcga catcgtttat cgtccctgag
     1141 atcatttcca ttgacgaaga gctgctgaga aagagcatcg atgaaatgga agggctttcg
     1201 ctgtacaggc actttttcga tgatatcctg aggcagaaaa agcatgtgct ttccgacaag
     1261 gaagaggaac tcctggctct cgcggatgaa gtcatggatt cgccctccga gatcttttcc
     1321 atgttcaatg acgcggacat caagttcggc acaatcaggg atgaaaacgg agaggaagtc
     1381 gaggtcacaa aaggcaggta tataaaattc ctcgaatcga aagacaggcg ggtcaggaag
     1441 gacgcgttcc acaccctgta cgattcatac agggcaatga ggaatacgct ggccgcgtcg
     1501 ctcggcagca atataaaaag ggacaaattc ttcgcaaacg tcaggcggta tggatcgagc
     1561 ctgcaggcag ccctggacag tgacaacgtg cccgtcgacg tgtacgacaa cctgatcgat
     1621 accgtcaaca ggaacctccc tctcctgcac aggtacgtga ggctgcggaa gaaagcgctg
     1681 aagctggacg aactgcacat gtacgacctg tatgttccga tcgtcgagca gccgacgagg
     1741 catataagct atgaggaagc gctggaaacc gttaaaaacg gcctggcggc gctggggtgc
     1801 tgttatgtcg aagacatgac gaaagctttc ggttccggct ggatagatgt atatgagaac
     1861 cagggcaaga ccagcggtgc gtattcgtgg gggacatatc tgacccatcc ctatatactg
     1921 cttaactacc agggcactat aaacgatgtc ttcaccatag cgcatgaaat gggccacgcg
     1981 atgcattcgt actatacaaa caaaacgcag ccgtatattt attcggatta caggatcttt
     2041 gtcgcggaag tggcctcgac ggtgaatgaa tctctcctta tggattatct gctctctgtg
     2101 accaccgaca aaaatgagcg ggcttacctg atgaaccatt acctcgagga attccgcggg
     2161 acgctgttca ggcagaccat gttcgccgag tttgaaaaga tcacgcatga aatgatagaa
     2221 aaaggcgagg cgctcaacgc cgacgggttc tgcaagatat accgcgggct taacgagaag
     2281 tatttcggcg ccgaagtggt catagacgac gatatcgata tggaatgggc gcgcataccg
     2341 cacttctaca gaagcttcta tgtttacaag tacgctactg gcatctcggc tgccgtatca
     2401 ctgtcgaggc agataatcgg cgacggcgtg ccggccatcg accgctacct ggaattcctg
     2461 aaaagcggcg gatcagacta cccgctggaa ctgcttaaaa aagccggcgt agatctgacc
     2521 gtgccgaagc ctgtcgagga tgccctgaaa acatttgaaa aggtactgaa cgaccttgag
     2581 gagatcattg gataaataaa tggtctgctt ttggctggaa tacgatatac agtaaaaaaa
     2641 gctcatgaaa agaggcggat gacgaatgac agatccgagg atcgaaacac tggcgaaaaa
     2701 cctggtcaat tactcatgcc gagtcaaacc cggcgagaag gtactgatcg aaacgaaggg
     2761 ctttgaactg ccgctgacga aagcccttgt aaaggaggtt tacaaagcag gcggcctgcc
     2821 ctttgtcact ataaagaacg acgagatcct gcgtgcgctc ctcatcggct gcagcgagga
     2881 gcagatcagg ctcatggcaa ggttcgagct tgacaggatg aaggagatgg acgcgtatat
     2941 cgggatcagg tatgcggaca attccaacga actcggcgat gtccccgaag ataaaatgaa
     3001 gctgtacagc acgctcttca acaagcctgt ccattcggag cagagggtgg agcacaccaa
     3061 atgggtgatc ctgcgttatc cgaacagcgc catggcgcag atggctgaaa tgtcgacgga
     3121 ggccttcgag gatttctatt tcagggtatg caacctggat tacgggaaaa tgtccagggc
     3181 gatggacgca ctcgtcgaac tgatgaacag gacggacagg gtcaggatcg tcggcaaagg
     3241 cactgatctg actttctcca tccgcggtat tcccgccatc aagtgcgacg gacaccggaa
     3301 catccctgac ggcgaagttt acacggctcc cgtaagggac tccgtcaacg ggaagatcac
     3361 gtacaatacg ccttctaaat accagggctt tacatatgaa aacgtgaccc tggagttcag
     3421 ggacggcaag atcataaatg ctgcggccaa cgatacggaa aggataaata aagtgctgga
     3481 tacggacgaa ggctccaggt acgtgggcga attctcgctg ggcttcaacc cgtatattga
     3541 aaagccgatg aaggacacgc tgttcgacga gaaaataaaa ggcagcttcc atttcactcc
     3601 gggcagctgc tacgatatct gcagcaacgg caacaaatcc gcgatccact gggacctggt
     3661 gtgcatacag cggccggagt acggcggcgg agaaatatgg ttcgacgatg tgctgattag
     3721 gaaggacgga gttttcgtac cgaaggaact ggaggcgctg aacccggaaa acctgatata
     3781 gaataatgca ggggccaaat gggcctgcag agagcggttt acggcagcat aaagcaaaat
     3841 ggcgcccgaa atggcagcca aagctgaagt atagttaaac cgtccctgaa aaacgaggtt
     3901 gtatggaaaa ctggatgatc ggcacaagat tcataatact gttctactgc gtgttgcgct
     3961 atttcaggag cagcatggag aataccgcgc ttgtggtatt gttcatgctg ctttacattt
     4021 ccgcagtcac cactgtttat ttgttaagga aaacctcgct gaaaaccctg ttccgtatgc
     4081 tctctgtaac tattctggca gtttcatcgg ttctggcgac cgaactgttt ctgctgctga
     4141 tgtctgcaga tatttatgag atcgcatcct catatattga tgactggagg ttcctgccgg
     4201 ttttcgccgc tgtgcccgcc cttctcagca ttgacaccgg cgtggtgctg gaatatgtgc
     4261 tggcaagcct gatgagcctg ctgattttcc tgatcgccca aaagcttatc caaaacgttg
     4321 gcgccctgaa ggaagccaat gaggaactca gggacagtat agacgacctg tccgtgagac
     4381 tgagtgcggg caccgaatat gaagcccagc ttcgctacct ttcgcagata gaagaacgca
     4441 acacgctggc gcagaagatc cacgatgaag tggggcatac gctggcaggc agcatcttac
     4501 agcttgaggc tgtcgggctg atcatggaaa aggacgccgc taaagcaggc gaaatggtga
     4561 ggaatgtgac cgaaaacctg aagaagggga tggaaagcat acgctccaca ttaagggcta
     4621 taaagcccgc ctcggagcaa ctggggataa acaggctgaa gctgatcctc gaggagttca
     4681 cactgaacaa caggatagag accgaatttt catatgaggg caggctggat gccataacgc
     4741 atatgcagtg gagcatcctg accgacaaca tgagggaagc ccttaccaac tctctgaaac
     4801 attcttcggc gtcagccgtc agggtgaaga tagaggtcat gaacaaagtt ataaagatgg
     4861 aagtcaaaga caacgggaga ggcgaagccg ccattaagaa agggatgggg atcgccggca
     4921 tggaagagag gacaggtgcg gcgggcggca agctgatcat tgacgggtcc gacggatttt
     4981 ccgtgattac tctgctgcct gttgaaaatg atccggaaag gcggatagct gccggacgga
     5041 caggaaagca gcagccagga gagcagcata tcagccgcac ggttgaccag tcaggaggtg
     5101 cttatgccga tcagggtatt gatagctgat gatgacgcat tgataagaga aggactgaaa
     5161 ataatcctgc agagcgacga ccgctttgag gtggcggaat gcgtcgataa cggctgcaag
     5221 gctgtggaat actgcagtac gaaccaggtg gacgttgcgc tgctcgatgt caggatgccg
     5281 gtaatggacg ggcttgaggc tgcccgtgag atctcgtcga aaaccgggac gaagacgctg
     5341 atcctgacaa catttgacga cgatgatttt attataaacg cggttaagta cggcgccagg
     5401 ggttatctgc tgaaaaacag tccgcctgcg aggataatgg atgccataag gatagtgcat
     5461 gacggaggca ccgtcatgca ggacgttgcc atggatatta tcaaggacga actgtcgtcc
     5521 gggcgaagat gcggaatccc ggacgggatc ctgaccgagc gggagatgga gatagcgatg
     5581 cttgttgcga aagggctttc caacaaggat atcgcggcaa ggctgttcat gtcggagggg
     5641 acagtcaaaa actacatatc cgctattctg gcgaagaccg ggctcgagca ccgtacccaa
     5701 ctggctgtgt attatctgac cggcgagaag gggaatgggg cgtagcaatc ccggtaccgt
     5761 ctggcgtaca ggcctttttt cacagtaaaa acattacttt tgtcatatga acctgtgacc
     5821 ttccgtacta acggaagaga acaggttttt ttataatatg gatacaatgg atagtatggc
     5881 tggaaccgaa cagcggccaa aaccattaca gaacaggatt attgcatgtt ggaggtatga
     5941 tgatgactat tgtcaaaatg gaacaggtga cgaaaaaatt cggcgatgtt actgctgttg
     6001 acaaagtatc gctggagata gaagaggggg agatattcgg gctgctggga cccaatggcg
     6061 cgggcaaaag tactgccatc aatatgatca cgggattgct tacgatcgac aggggcagta
     6121 tcagcatatt tggaaaagat gtcaggaaag acaaaatgac gaccaagtcc tacgtgggca
     6181 tcgttcccca ggatatagcg atatatgagg acctgaccag ccttgagaat gtgaagttct
     6241 ttgccagcct ttacggcctc agggggaaga tgctggagga agcagcactg gaggctcttg
     6301 agttcacggg cctgtccgaa aagaaaaacc agtttccgaa gagcttttcc gggggcatga
     6361 agaggaggct caacatagcg tgtgccattg cccacaagcc aaaactcatc ataatggacg
     6421 aaccaaccgt cggtatcgac ccgcagtcaa gaaaccacat actgcagtcg gtaaaaaaac
     6481 tcaacgagtt gggcagcacg atcatataca ccagccacta catggaagag gtcgaggaga
     6541 tatgcacccg tatcgccatc atggaccacg gaaaggtgat agctcttgga acatgtccgg
     6601 aactggaaaa catgataaac gacaggggcg tggtcgtggt gacgataaac gaacccgctt
     6661 caaaggtcga tgagaacagc ctcagggaga tccccggcgt agaaaatgtc gatataggtg
     6721 aaaacacagt aaaaataaca agcgccaagg aagtcaccaa cctggacaag ataatacagt
     6781 atttcataaa aaacagcttc acgatcaaaa atgtggaaag taaaacgcct gacctggaaa
     6841 ccgtgttcct gtccctgaca ggaaggaagc tcagggactg agggaggtgg cttgcaagtg
     6901 atcatactga acatcatata caaggaacta aagcagtgtc tccgggattt taaaacaaat
     6961 atcctgatgg tgatctttcc catagtgcta atcgtcatcc tgggagcggc tttcagcggc
     7021 gttttcgaaa acagcatcga tattgaggac gtcaatgtac tgtacactat cgatatagag
     7081 ggcggcgatc ccgtatttga aaatgctttc agcacattct gcgagtacat gtctgatgag
     7141 acagggatgc agttcgaaga gacagcagat gtcgaaaaag gtataagcga aatagagaac
     7201 cacaggtata cggcatatct ccacataaca ggcgacccgc cccggataga catgtacagg
     7261 aacaaaaact ccggctttga atcagggata gttgaaaatg ccgtggcatc tttcctcgag
     7321 tcatatgcgg caatcaatgt catagtccga aacgacccgg ctgccctttc aaaagtacat
     7381 cagcctgaca gcgccgcgaa cagtggttat gtcagggtca ctgccataga ccgcaagaag
     7441 cagccaggtt ctaccgatta ttatgcggtg acgatgctga cgctgatcct gatgtattct
     7501 tcactcacgg gattctggag cataaggtct gaggccgatg cgaagaccgg cgccagggtg
     7561 ctctgtgcac cggtccaaaa ataccagtac ctcaccggaa agatacttgg cagcatttct
     7621 gttaccatcg tacaggcgtt tgttgttctg ctgttcagca aactggtgct gaaagccaac
     7681 tggggagaag acctgcttac cgtggcggtc cttatcatca catacgcgat aatgaccgtc
     7741 agcctcgggg cggcgctggc attcatattc cgcacggcag aagtggcaag cggcctgatg
     7801 aatactatca taccggtatt cgtatttctc ggcggaggat atgtgccttt agccgtgctt
     7861 ggcgaccaaa tttcaaaaat atctgccatt tctccggtga gatggatcaa catggccctg
     7921 ataaatataa tttacgaggg cagttactct gacgcgtata tttcccttgc agtcaacctt
     7981 ggactttcca tactgttcat atccgcggct gcatggctgt caggaaggag gaagaatgca
     8041 tatgcgtaac ctggggcttc tcatactgaa caacctgaaa gtgacgttca gaaaaaaggg
     8101 caatattatc acatatatct ttctgcctgt ggtcggagta ctgttttcgc tggtaataca
     8161 cagcacgtca tctgtcagta tcctcaatct cggctttact gaccacgacc gcggaatggc
     8221 ggcccttgaa ctgaaggaaa aactggctgc aacagaagat ttttcggtca tcgatgtgga
     8281 tgaggaggaa atcagcaaaa agctgctgga ttttgaactg gatgcagtgg tggtcgtccc
     8341 tgaaggatac actgagagca tcctcagcgg gaatgccgct gaaatagaga tcgtctcgct
     8401 caagggcaag gaaacgacgg catgggtgga acaactgata tacaggcatt cagcttccat
     8461 gtcgctgctt tcggctgcat ccggcggcga tcctgcgact tttgacagga tgctctcaca
     8521 ggtcagtgat aataaagtgg aattcaacgt tattaacgct gaggacagga gcactgataa
     8581 ggcgatgacc cggacttcga tcgggtttct ggtcatgttc gtcatgcttg gttccggatt
     8641 tacatcaatg atcgtcctga aagaaaaaag agaccgtacc taccacagga tctgttccgc
     8701 gccggttagc tccaggcaat acatttacgc gaattcgctg accgggctga tcatctgcat
     8761 acttcagatc gtaatgatac agttgataat gaagttcgtc ttcaggatcg atgccggtat
     8821 gagcgatttc catttgttta ttatactgct gatgttcgcg ctcgttgccg tcggcctggg
     8881 gctgctcatt accgcgtttt ccagttcatc atacatggcc gggacgctca acacgcttgt
     8941 cctgacgcct acctgcatgc tgggcgggtg ctactggaac cctgaactta tgcccaggtt
     9001 catgcagaag atcggttact ttacccccca gatgtgggtg atggaggcta tcaggaaaat
     9061 gcaggacgga ggcgccctcc ggagcattac gctgaacctg ctggtacttg ccgcatttgc
     9121 gctggcgttc atgctcatcg ctgcattcag gctgtccagg acagacaact tacagaaatt
     9181 tgtttaaggg acagttctca acttatgcgt ccaggggaca gtcctcaggt ttccatggca
     9241 gagggaacag tactcgggtc tatgaggctg tggagcagta caaagttctg catgtcaatg
     9301 aggcattttt gataagacga agggacagtt cttcgaccgt gaccgccgcg gtcatgcttt
     9361 tgaagaactg tccccttctt ttatccagaa aaaacgtccc tgaaactgcc gttaatttgc
     9421 agaaccgtcc cctgttattc gaaatattcg atatcaagca gctttataat tcccaattct
     9481 gcatttacag tgtacatata tctcatgatc gccccggatt tgattataag ttctctcagg
     9541 tcggaatagc tgtacctgga ataatcggat accttgattt caatgaccgt actgcggttt
     9601 tttatagctt cggccaggac cctcttcatt gcttccgagt tgtcagccac aaggccgtat
     9661 ttacgatagt aattgttccg ggtggacgta cacctgggga acagggacgt attccagcta
     9721 tagaattttt tcatttcctt gtcggtcagg ttgaaatagg tgtaggtcag tacttcggtg
     9781 ccgtcatccg ttattgcatc gtcgttggtc acatcaaggt ggtagtattc gccgtcgatt
     9841 ttgacgatat tccaggaatg cccttctctg ctgctgtctg tggaccaacc ggttgctatc
     9901 atgcaatcga tgcctgccag gtcgcacagc agtttcatag cctttgcata gcccgaacat
     9961 acggcttttc ccaaaacaag gcagccgtat tcgttgaatg agtcttcagg cacagtgttc
    10021 cttttgtaat ttatataatc ataacgtgtg ttgcttacca aatagtcatg cagtaccttt
    10081 tctctctcaa aatctgtcat atcccttgag atacgttcgg cgacgatgta ccttgctttg
    10141 ttgaacgcag cctttacttt cgacctgctc aatgtaaatt cgtccgccgt ttccctgtat
    10201 gtgaccgtca ccttcatggt gtgcgaattg cattcgtatt tccacgattc catgaaattt
    10261 tccactcccg atataacagc tgcagcgtcc cgggctatgt cgacagcaga cctgaactct
    10321 tccgatatat cgccgttgta gccggtgatg tccaattcaa aggacacggt atggctgacg
    10381 aacgattttg tgatgatcct gaccaggtcg tctgcattcc tgacgacata gccgtagttt
    10441 gtacggcggg gactgaatga aagaacattt ccataccgct tttcaaaatc cggcgggtac
    10501 aggccaagta gtacagcggc gggcttgaag actgtcctgt cggtatcggc aagcttttct
    10561 atgagcatcc gggatgaacc tttcagtttt gccgtaagtg cgttatagga aacggcgaca
    10621 agatcgttcc tcaggaactc ggacctgttc ttcagatcct ttgcttccgc atttgacaaa
    10681 attcccaatg atacagcttt gtccaatgcc ttgttgtatt ggaaatcttc tccgtccctg
    10741 tatccgatgg aacgaagtac cagcgttaca tattgctctg ccgtcagcgg atcatgtgcg
    10801 ccgaacgtat ctttgctttt accgttggtg atcccgttct gatacgcata tcccacatag
    10861 ctgtccgccc aggcaggaac atctttgaac gggtgtttgc agccgccctt cagggcctcg
    10921 gattcttttc cgagaagccg cagcagcatg gcaagccctt cagccctgtt cggagcgcgg
    10981 tcaagctcaa agtctgcggg cttgttggcc agaaggccca gtatcttcag atcgatcgct
    11041 ttttcataat tcgactgggc tgccgataca gtttttacgg acagcaactg ggcagcctgt
    11101 gccggccgtg atgcaacggc tgccggcatg ggaacaaaca tcagtgttag cgaaaatatc
    11161 aaaactgcgg ctaaaacttt cggtttcaat tatgtatact acctctctga actctttggt
    11221 ttatattatt attcgtatta atattcgtaa acgacaagta aaaaaccggg gtaattttcc
    11281 tgcttttagc tcctgataag aataaatcgt cgcgaatcag caattattat agacgatggt
    11341 acataaatat gcaacatttt gatgaatttc tgtctccgat gtttgtgaaa ggctcggaat
    11401 gcagattgca gagccaagaa tagcttttgg taaaaagcag cggcatcttt attttctgta
    11461 tctatattca gcacaaacga gtacactccc cattccttgc acaacaaaaa ggacagatat
    11521 caacagacca caacagggca gttgatccgt ccctcaagag gcatggaaca aagagcagct
    11581 ttagctgtct gccgggcagc ggcttaccgc aatgttcagt tattccttgt aatacccttc
    11641 attttctctt ccgaaaaggc aagggaacaa caggattata accaatatga ttatgatgat
    11701 ccagatccag ttaaatccga aaccacaccc gaagagcccg cctctttcat tatctgccat
    11761 atcatacacc cccttttata ccgctaattt agtttattcc tcttccgtgc aaagtgttac
    11821 agatccgggt
//
LOCUS       DAOSCC010000053        11656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3702_length_11656_cov_5.756056, whole genome shotgun sequence.
ACCESSION   DAOSCC010000053 DAOSCC010000000
VERSION     DAOSCC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_11656_cov_5.756056"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4723)
                     /locus_tag="PLG72_04390"
     CDS             complement(<1..4723)
                     /locus_tag="PLG72_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HOJ80070.1"
                     /translation="MKTIRKPVAVFLAATVLYGIFSIFGTVSFAAPDSVNISVASYSD
                     GKLTIRWDMLSDARSAEITYHRPGTGGTATFQKEIVAAENTYTIEGLQADYIYDICVT
                     LYNNVDPSAGEAIGRGLLYFLPAITFTATAPKQPYEDIPGGGREIGGTPKLRLNWKQP
                     RMYYDSSGTATYEPGSGTFVGANSEEALDYMRDSYKNIYDDRDWDKLSLNFMINISTD
                     ANKLDSGSSVIIEQQADFNSYSAYVSGTPAKKAVIPGPNASSGYYSFELLGRKDEKTD
                     ISNDVDGDDILPHNGIFPGTVYYMKIIPIYSDSNGDNVPAVRVGDPDDKNWSVLAGSK
                     PYISTPIRFQLTKDSFNNIYVKIYRINQGSLDLPRLFYQVQSSVDDTIPGDWHILDTL
                     DDSYFSGEYAVTVLTGVSPDNKVFYKIVVKSEGAEDRLESLPLEYMLVADTDRPPLPT
                     GVTVIDRVLNEKEVTDPSGSVRKVKSTDITISWEKPINWDAVKDDLVYHFLLSTSQTD
                     PGNGAKVPVYVDGKLWGRSEGYESKYRLVKYISANSGRIREVGNRLELTLDGFELFDL
                     DGDPATPPVLGTDDEGYPTFLIPNTVYYIQMYSTKATDAGTSDRDKMSDLSVVVSFTT
                     LAGFETDVPLPPHFSLGGNGKLTSGGKVLNYIDLSFSKVQDLDWRNYTSEYDESKYQY
                     DIYYDIFMNMGANRLSFIPVGTTQELQGDVLFTGADDPRSSVVTARIWQFTDDGYNKL
                     KDVLPNDATSNPADKFGSNLLPNTTYYFLIRTRLVITNKQDPDDIVLEKTSMFSSLLP
                     VTTTILDVTPPDDSQRRPLAPTDFSIAVDAEGEQLLSDSSVTFSWTHREKDVIYQIVR
                     TTGRVHPMAEQSEYENDPEYLSFLDDYDPLQSGGVFIDPEGDPQQNFPGKFTYDSSTG
                     ICRFTVDRGMFPNRLYYFSIKAIKVGADRKPVTPPSHSVWVSVPVTTSFIEAPSMLEA
                     VPGAEIGFFWTDSSPAASAEDYTIWLKGPGDAAYKPMSKAQATIIRDPDRKTYYGRIT
                     GLKHGSSYSVKVTRGSGTTVYEKTDFTTRDSYHELEIKWMGKLLDDYASYEIAIMEEG
                     GSEYTVLTAADLEWYIDKNGSVLPYYTEETARTVGNDRLYYHARIKSMDVTLPGGIVT
                     KQPLKSNTRYYIKVRSKKRDPVYTDIISYSKYIGPVSIRTEFSQEDYDNTDREEQQRA
                     EFLEKMDQLERGYFWRIAIGTNNITSIMLKGDRVADAMRNSPGDSFMIDISEISANIE
                     RDEIYIPVSLLNVMKSIKKSLIIRSDGTEVVLRPYTLDAVFNETVKGIRERQEVKELY
                     IKLDLRRENRTDKTLPSGMIPVSAVNGLGIQAMGFSIAYEDMASLFHDRLYNKDTGLV
                     GEKLSMLLNTYVGSGAGSASLIDQYTGNLVNLIEKELSVYIDNTIKAIKLTNTVREIN
                     AFDSPVSVRLKTGESGELKSAYVKYNGMDSWRKATISEAGSIVALELLKPGTFVILVP
                     DRSFGGIPEGHWARDYIVKMASKYDLESVFQGARTNFMPEVNVTCREIVLLYELTTGK
                     APANTGLNIKTRLTELGLDDLL"
     gene            complement(4953..5348)
                     /locus_tag="PLG72_04395"
     CDS             complement(4953..5348)
                     /locus_tag="PLG72_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80071.1"
                     /translation="MDYQKHDNGNTKGRANYGRGRGGNEAGRERRGPADGGFDISTDS
                     DPLHDRTLNGRIVNAESLQIDATLNEISGELKPGETLILDIGNNYFEQTDEIYDTLVL
                     RGYNVRKTFRNGRNQIIVSRGDQDRELRH"
     gene            5451..5963
                     /locus_tag="PLG72_04400"
     CDS             5451..5963
                     /locus_tag="PLG72_04400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ80072.1"
                     /translation="MAGGRNLVLIGMPGAGKSTVGVLLAKTLKMPFTDTDILIQKQEN
                     SYLQELIEKHGIDGFIKIEENTVKSLDLRNHVIATGGSVVYSETAMAHLKAHGIIFFL
                     NARLYQLERRLKNSRNRGIAMSSGQTLGSLYRERLTLYKKYADIEIDCSRKHIETIVS
                     EISEKFLDLA"
     gene            complement(6092..8221)
                     /locus_tag="PLG72_04405"
     CDS             complement(6092..8221)
                     /locus_tag="PLG72_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ80073.1"
                     /translation="MKRRTEKVLNFFGDAAANAVNAVKNAVKNTGGALNRSGNVLRII
                     KIQSRLIAAFLVLSLLPLSLMGLISYSRSNRAISERIKKYSTGLINQTAVNLESELVK
                     YEQLVREIAYTDSIQQSFTDMRGLSAEEKLRYSFQISNTIREKCATYNEIAQFGFIIE
                     NDTNRAFLKDAKEITDQETKRIYEKAKEARGAPVWLITETSAGNILLVAAREINNTKS
                     GGKIGVFIIGLDTRYFREKLENVDLGSGSQLFMMDADGTVLVSRNEDVAINRKYSDPA
                     ILDMLVSGREQGTSTFDFDHNLAVYSYLKDHDWYVVSLIPYRYINAAGNSILTTVIIL
                     FIVFVVVSTLLSFGVSSSISVHLKKLVNVMQEARGGNLALHLEDRSNDEIGIVVRHFN
                     DMLENIRGLIAKVSDSSADLIDSIGKIKTSAESTRTASEQIALTMQEIAKGAGEQAEE
                     ISYGVTCTNRLSEGINKVGESMETVSSVINDTKELSERGLSVVMMLKEKARQTNDITR
                     KMAEDIRSLNADMKQIRKIANAIGTIAEQTNLLALNATIEAARAGEAGKGFSVVASEV
                     KKLADKSKESTAIINSIINSIQAKADSTVITAETGSKAVDEQMEAVNSTDEAFKNIYS
                     SMDEIIENMKVMHESVENMNASREDTVRVINNISAISQETAATTQEVSANSQEQMSAA
                     EELAVLAEKLDDMAQELKKAISIFKVE"
     gene            complement(8547..11063)
                     /locus_tag="PLG72_04410"
     CDS             complement(8547..11063)
                     /locus_tag="PLG72_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ80074.1"
                     /translation="MENSLVKFSEQGAATISEFIHGRLADVRSMAENSIIRNSSLPVE
                     QRLDELRAQLKLDSYRRLSLADLDGNAVSTDGITLNVYDREYFQNALKGKPSVSDPII
                     SRADGAIVIVFASPVFESGEVTGVLYVTYDADVISKIADNIRFSDMGYTFILNRNGDI
                     IAHRDRALVYGRVNHISENKPELGKLIELEKRMIAGEKGAGEYYYEGEKRYMGFSAIE
                     STGWSIAVTAPKGDVFRKLNIVSAILLVCILIASFIIAYIMSRSTLLKEDLQRQQVNT
                     HRIADFTNLIAMTIRTDGTILTTNRYAEDLLLYFDKFGEEKVNNIFELISDSDRNMLE
                     NTVGATREQDSSTSFDLALSRGDSKVVHVYCSAITEKDNANIIEIIGIDITDRVMQEN
                     ILQDSFEELTVVYDELAANEEKIRQLAFKDQLTGLSNRTELLNEMEKVFSKFREGERC
                     ALLYLDIDNFKFINDSFSHSVGDMLLVEIGKRLRNAFTESELVARFEGDEFVVFVRNF
                     GSPDELNEKISTAVGIFNEPFNVMRNTFHISASCGICVYPDQVNSIEEMMKSSDVAMH
                     HAKKEGKNTHMMFRQEMNDELVERMAMENGLRNALANNEFILFYQPQVELSTGEIASF
                     EALLRWMHPEKGMIQPLKFISVAEESGLIIQIGKWALKTACEFITRLNSRTPKKYGIS
                     VNISVVQLMQADFVDMVREILETTGLEPSLLELEITESKLVEAVDMNLKKIHELRELG
                     VRFSIDDFGKGFSSLSYLKQLPVNTLKIDKCFVDDIPDNDNSMIESIIHIGHKRNLVV
                     VAEGVEKKEQMEYLTKHKCDMMQGYYYSKPVPEEEVHRLL"
     gene            complement(11352..11591)
                     /locus_tag="PLG72_04415"
     CDS             complement(11352..11591)
                     /locus_tag="PLG72_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ80075.1"
                     /translation="MKSTGIVRKVDELGRVVLPIELRRTLDIAEKDALEIFIDGQNII
                     LKKYEPACIFCGDARDVVNYRGKNICSACMKELRG"
BASE COUNT         2651 a         3004 c         2613 g         3388 t
ORIGIN      
        1 gcagcagatc gtcaagtcca agttccgtca accgggtctt tatattaagt cccgtattcg
       61 ccggggcctt tcctgtcgtc agttcataca gcagcactat ttccctgcag gtcacattta
      121 cttcaggcat gaagttggtc cttgctccct ggaaaacact ctcgagatca tacttggaag
      181 ccatctttac tatgtaatcc ctggcccagt gtccttccgg gatgcctccg aagctcctgt
      241 caggcacgag gatcacgaat gtgcccggct tcagaagttc gagagcaacg atcgaacctg
      301 cttcactgat cgtagccttc cgccagctgt ccatgccgtt atatttcaca tatgccgact
      361 tcagttcgcc tgattcgccg gtcttcagcc ttacggaaac cggggaatcg aaggcattga
      421 tttccctgac ggtgttcgtg agttttatcg ctttgatagt attgtcgatg tatacggaca
      481 gctctttctc tatcaggttc accaggttgc cggtatactg gtcgatcagc gaagcagatc
      541 ctgcgccgct tccaacatat gtattcagaa gcatgctgag tttttcgccg accagtccgg
      601 tgtctttatt gtaaagcctg tcatggaaca atgaagccat atcctcatat gctattgaga
      661 atcccatcgc ctggatcccg agcccgttta cggcggacac ggggatcatg ccggacggaa
      721 gggtcttgtc cgtacggttt tcacgcctga gatcgagctt gatgtaaagc tctttcactt
      781 cctgcctttc cctgatgccc tttacggtct cattgaacac ggcgtccagc gtataaggcc
      841 tcaatacgac ttcagtgccg tccgacctga ttatgaggct ctttttaatt gatttcataa
      901 cattcagcag cgaaacaggt atgtatatct cgtccctctc gatgttggca gaaatttctg
      961 aaatgtctat catgaaggag tcgcccgggg agttcctcat ggcgtcggct accctgtcgc
     1021 ccttgagcat tatactcgtg atattattgg tcccaatggc gatcctccag aaataccctc
     1081 tttcaagctg gtccatcttt tcaaggaatt cggcgcgctg ctgctcttcc cggtccgtat
     1141 tgtcatagtc ctcctggctg aattcggtcc ttatgcttac ggggcctata tatttcgaat
     1201 atgagattat atccgtgtag acaggatccc tcttcttcga cctgactttt atataatacc
     1261 gcgtattgga tttgagcggc tgtttcgtca ctatgcctcc aggcagcgtc acatccatgc
     1321 ttttgatcct ggcatggtaa tacagcctgt cattgcctac ggtccttgct gtctcttcgg
     1381 tatagtaggg cagtacggaa ccgtttttgt ctatgtacca ttcaagatcc gcagcggtga
     1441 ggacagtgta ctcggatccg ccttcctcca ttatggcgat ctcatagctt gcgtagtcat
     1501 caagaagctt gcccatccat tttatttcaa gttcatgata gctgtccctt gtcgtgaagt
     1561 ccgttttttc atagacggta gtgccggaac ccctcgttac cttcacgctg tatgatgatc
     1621 catgtttcag gcccgtaatt ctgccgtaat aggtttttct gtccggatcc ctgatgatcg
     1681 tcgcctgcgc ttttgacatc ggcttgtacg cagcatcccc gggtcctttc agccagatgg
     1741 tgtaatcttc ggcagaagct gccggagagg agtctgtcca gaaaaagccg atctcagctc
     1801 ccggaacggc ttccagcata ctcggagcct ctatgaacga tgtcgtgacc ggaacagaga
     1861 cccaaacgct gtgagacggc ggtgtgacag gttttctgtc ggctcccacc ttgatagcct
     1921 tgatgctgaa gtaataaagc ctgtttggga acatgccgcg gtccactgtg aatctgcaga
     1981 taccggtgct gctgtcatat gtaaatttac cgggaaagtt ctgctgcgga tcgccctcag
     2041 gatcgatgaa aactccgccg ctttgcaggg gatcataatc gtcaaggaag ctcaaatatt
     2101 ccgggtcatt ctcatattcc gactgttcag ccatgggatg caccctgcct gtagtcctga
     2161 ctatctggta tatcacatct ttttccctgt gggtccacga aaacgtcacg ctgctgtcgg
     2221 acagcaactg ctctccttct gcatccaccg cgatggagaa gtccgtcgga gccaacggcc
     2281 tgcgctggga gtcatcggga ggagtgacat cgagtatcgt ggtcgtcacc ggaagcagag
     2341 acgagaacat cgaagtcttt tccaatacta tatcgtcagg atcctgtttg ttcgtgatca
     2401 caagcctggt cctgatgagg aaatagtacg tcgtattggg aagcaagttc gatccgaatt
     2461 tatcagcagg attcgatgtt gcgtcgttcg gcagcacgtc tttcagcttg ttgtaaccat
     2521 cgtcggtaaa ctgccagatc ctcgctgtta cgacagaact cctcggatcg tcggcgccgg
     2581 tgaacaacac atccccctgc agttcctggg tggttcccac aggtatgaag ctcagccggt
     2641 tcgcgcccat attcatgaaa atgtcgtaat agatatcata ttgatatttt gattcatcat
     2701 attcactggt atagttccgc cagtcaagat cctgcacctt gctgaaactg agatctatat
     2761 aattcaggac ctttcccccg gaagtgagct tgccgttgcc gccaaggctg aaatgcggcg
     2821 gcagcggtac atcggtctcg aaacctgcca gcgtggtgaa gcttaccacc acggacaggt
     2881 cggacatttt gtcacggtcg ctcgttccgg catccgtcgc tttagtggaa tacatttgga
     2941 tgtagtaaac cgtgttgggg atgaggaatg tcggatagcc ttcatcatcg gtcccgagca
     3001 ccggaggtgt ggccgggtct ccgtcgagat cgaacagctc gaacccgtcc agagttaatt
     3061 cgagcctgtt tccaacttcc ctgatcctgc ccgaattcgc tgatatgtat ttcacaagcc
     3121 tgtatttgct ttcatatcct tccgacctgc cccacagctt tccgtccaca tataccggca
     3181 cttttgcacc gttgccggga tctgtctggc tcgtgctgag caggaaatga taaacaagat
     3241 cgtccttgac cgcatcccag tttatcggct tttcccacga tatagtgatg tccgtcgatt
     3301 tgaccttcct gacagacccg gagggatcgg tgacttcctt ttcattgagc accctgtcta
     3361 tcaccgtgac gcccgtcggc aaaggaggcc tgtcggtatc tgcgacgagc atatattcca
     3421 atggcagcga ttcgagcctg tcttcggcgc cttcagattt gacgactatc ttgtaaaaga
     3481 ctttgttgtc gggactcacg cctgttaaca cggtaacggc atactccccg ctgaaatatg
     3541 aatcgtcaag ggtatcgagt atgtgccagt ctcccgggat ggtatcatcg accgaagact
     3601 ggacctggta aaaaagcctg ggaaggtcaa gacttccctg gttgatcctg tagattttca
     3661 catatatgtt gttgaaactg tccttggtca gctggaaacg gatcggcgta ctgatatacg
     3721 gctttgatcc ggccagaacg ctccagtttt tatcgtccgg gtcaccgacc ctgacagcgg
     3781 gcacattatc tccgttcgag tcgctgtata tgggtattat cttcatataa tatacagtac
     3841 ccggaaaaat gccgttatgc ggaagtatat cgtctccgtc aacatcgttg cttatatccg
     3901 ttttttcatc ttttctgcca agcagttcaa aactgtagta cccggaagaa gcattggggc
     3961 ccgggatcac tgctttttta gccggtgttc cggaaacata tgcagaatat gaattaaagt
     4021 ccgcttgctg ctctattatt accgaagacc ccgaatcaag tttgttggcg tctgttgata
     4081 tatttatcat gaagtttagc gacaacttgt cccagtctct gtcgtcgtag atatttttat
     4141 aggaatcccg catgtaatcg agcgcctctt cgctgttggc cccgacaaaa gtaccgctgc
     4201 cgggttcata agtggccgtg cccgaactat cataatacat cctgggctgt ttccagttca
     4261 gcctcagttt cggcgtaccg cctatttccc tgccgccgcc gggaatatct tcatagggct
     4321 gcttcggcgc agtcgcagtg aatgtgatgg ccgggagaaa atagagcagg cccctgccta
     4381 tggcctctcc ggcagacgga tcaacgttat tgtacagcgt aacgcagatg tcatatatgt
     4441 aatcggcctg caggccttca atggtataag tattttcggc tgcgacgatt tctttctgaa
     4501 acgtggcggt cccgcctgtg ccgggcctgt ggtaagttat ctcagccgaa cgtgcgtccg
     4561 aaagcatgtc ccagcggatc gtcagctttc cgtcactgta acttgccact gaaatgttaa
     4621 ccgaatcggg tgcagcaaaa gaaacagtgc cgaatatcga aaaaatgccg taaagtacag
     4681 tcgcggccaa aaatactgca acaggtttcc tgatagtctt catacgatgc cgacctccgc
     4741 caatatatac gacccgatcc caggtctttc atacttttat gctatactgt ccctgaagtg
     4801 atcaagggta cagtccatat tatatatcgg catcgcatcc gcatggtttt atagcgctgt
     4861 gggctttgga tcggcccggt atcataatgt aataatactg taatgaattt gtaaatgttt
     4921 cggctgccgt gatatgcttt ggcaggagag ccttaatgcc gcaattccct gtcctggtcc
     4981 cctctgctta caataatctg gttcctgccg ttcctgaaag tttttcttac attgtatccg
     5041 cgcaacacga gggtgtcgta tatttcatcc gtctgctcga aataattgtt gcctatgtcg
     5101 agtatgagcg tttcgcccgg tttgagttcc ccggatattt cattgagagt cgcatctatt
     5161 tgcaggcttt ccgcgttcac gatccgtccg ttcaaagtcc tgtcatgcag gggatccgaa
     5221 tctgtgctga tgtcaaaacc gccgtccgcc ggtccgcgtc tttcccttcc ggcctcattg
     5281 ccgccccggc ctctgccgta gttagccctc ccttttgtat taccgttgtc atgtttctga
     5341 tagtccatat gaagtaccct ccgatgcttg gtttaaatcc tgtttttacg gtatatatct
     5401 atgttatagt attatcatga gacgattatt ttattttggg agttggattt atggcaggcg
     5461 gcaggaacct cgtgctgatc ggcatgcctg gtgcaggcaa aagcaccgtc ggcgttttgc
     5521 tggcaaaaac gctgaagatg ccgttcactg acactgacat cctgatacag aagcaggaaa
     5581 acagttattt acaggaactt atagaaaagc acggcataga cggatttata aaaattgagg
     5641 aaaacactgt gaagagcctc gatctcagga accatgtgat agcaacaggc gggagcgtgg
     5701 tatacagcga aactgccatg gcccacctta aagcgcatgg tatcattttt ttcctcaatg
     5761 ccaggctgta tcagcttgag cgcaggctga agaattccag gaaccgggga atcgcgatgt
     5821 cgtccggcca aacgctcggt tccctgtaca gggaaaggct gacgttatac aaaaaatatg
     5881 ccgacatcga gatagactgc tccaggaaac acatagagac catcgtttcc gagatcagtg
     5941 aaaaatttct ggatctggct taagaattta taaaagaaat gtataaaagg attcaaaagg
     6001 gggcaaaaat attagtgacc tcacaaaata cattttgacc ccctttacac ggttagtcgc
     6061 ctgactaacc accttttttt gccttttttc actattcgac cttgaatatc gatatggcct
     6121 ttttgagttc ctgcgccata tcgtcgagct tttcggcaag taccgcaagt tcttccgctg
     6181 cgctcatctg ttcctggctg tttgccgaca cttcctgtgt ggttgccgct gtctcctgcg
     6241 atatggcgga aatattgttt atcactctta cggtatcctc cctggaagca ttcatattct
     6301 caacggattc gtgcatcacc ttcatgtttt cgattatctc gtccattgaa gagtagatat
     6361 tcttgaaagc ttcgtccgtg ctgttcactg cttccatctg ttcatccacg gccttgctac
     6421 cggtttccgc agtaatgacc gtactgtcgg ctttcgcctg gatgctgttt attattgaat
     6481 tgattattgc cgtcgactcc ttggatttat cggcaagctt cttgacctcc gaagctacga
     6541 ccgagaatcc cttgccggct tctccggctc tcgccgcttc gatggtagcg ttcaatgcca
     6601 gcaggttcgt ctgttcagct attgtaccta tggcgttggc gatcttcctg atctgtttca
     6661 tatctgcatt aaggctccgg atatcttccg ccatcttcct ggtgatgtca ttggtctgcc
     6721 gtgctttctc cttgagcatc ataacgactg aaagccctct ttcggacagt tccttcgtat
     6781 cgtttataac ggacgaaacc gtttccatgc tttcgccgac cttgtttatg ccttccgaca
     6841 gccggttcgt acaggtcacg ccgtaggaga tctcctcggc ctgttcaccg gcgcccttcg
     6901 ctatttcctg cattgtcagc gcaatctgct ctgatgcggt acgggtactt tcagccgacg
     6961 tctttatttt accgatgctg tcaatcaggt cggcggacga atcactgacc tttgctataa
     7021 ggccgcgtat attttccagc atatcgttga aatgacggac cacgatacct atctcgtcat
     7081 tgctcctgtc ttcaaggtga agcgcaaggt ttccgccgcg tgcttcctgc atcacgttca
     7141 cgagtttctt caaatggacg gatatgctcg acgatactcc gaatgaaagc agtgtagata
     7201 ccacaacgaa tacgatgaaa agtattatga cagtggtgag tatcgagttc cctgctgcat
     7261 tgatatatct gtaaggtatc agcgaaacga cataccagtc atggtccttg aggtatgagt
     7321 atactgcgag attgtgatcg aagtcgaacg tggatgtccc ctgttccctc cctgatacca
     7381 gcatatcaag tatggcggga tcgctgtact tccggttgat ggccacatcc tcgttcctgc
     7441 tcacaagtac cgtgccatca gcgtccatca taaacaactg cgacccggag cccaaatcga
     7501 cgttctccag cttttccctg aaatatctcg tatcgaggcc gataataaaa acgccgatct
     7561 tcccgccgct ttttgtattg tttatttccc tggctgcaac gaggaggata ttgccagccg
     7621 aggtctctgt tatcagccag accggcgctc cgcgcgcttc ctttgccttt tcatatattc
     7681 tttttgtttc ctggtccgtt atttctttcg cgtctttcag gaatgccctg tttgtgtcat
     7741 tttcgattat aaatccgaac tgcgctattt cattatatgt tgcacatttt tcccttatgg
     7801 tgttgcttat ctggaacgaa tatctaagtt tttcttccgc gctcagtccc ctcatatcgg
     7861 tgaacgactg ctggatcgag tccgtgtagg cgatttccct gaccagctgc tcatatttta
     7921 caagctctga ctcaaggttc acggcagtct ggtttatcag tcctgtggaa tactttttta
     7981 tcctttccga aatggccctg ttggaccggg aatatgaaat caggcccata aggctcagtg
     8041 gcagcaatga cagcacaagg aatgcagcga taagtctgga ctgtattttt attatcctga
     8101 gcacgtttcc ggatctgttc agagctccgc cggtattttt gactgcattt ttcactgcat
     8161 tcacggcatt tgctgctgca tccccaaaga agttcagaac tttttccgtc ctgcgtttca
     8221 tattatcatc tccaccccct ggaaatacaa actcttaagt agtccattcc atgatggctg
     8281 gtcttagctg catgataatt ccaatggagt aattcgtcaa aataatacaa attccttctt
     8341 aaatatgcaa ttatttaccg tgcaggacgt cgaaaaaaat acgcacagaa cgtccgtttt
     8401 gtggaaatat gcctgtggat aatgttgata agtctgtgca taaccgagtt ataaagactg
     8461 caaagttgtg gataatatac tggtgcgtag ataattcaca aaaactgtcc acaggacagg
     8521 tctgccgccg gccggcgcat gcctgttcaa agaagcctgt gcacttcttc ttcaggaacc
     8581 ggctttgagt aataatatcc ctgcatcata tcgcatttgt gcttcgtcag gtattccatc
     8641 tgttctttct tttcaacgcc ttccgccact acgacgaggt tccttttatg cccgatatgt
     8701 ataatggatt ctatcatgct gttgtcattg tccggtatgt catcgacaaa gcacttgtct
     8761 atcttgagcg tgtttacagg caactgcttg agatagctca gcgaagaaaa tcctttgccg
     8821 aaatcgtcga tggaaaacct gactccgagt tccctgagtt catgtatttt cttaagattc
     8881 atatcaacag cctcaacaag ttttgattcc gttatctcaa gctccagaag ggacggttcg
     8941 agccctgtcg tctcaagtat ttccctgacc atgtccacga agtccgcctg catgagctga
     9001 acgactgata tatttaccga tataccgtat tttttcggtg ttctgctgtt cagcctggtg
     9061 atgaactcgc acgcagtttt cagggcccat ttgcctatct ggatgatcag gccgctttcc
     9121 tccgccacgc ttataaactt gagaggctgt atcatgccct tttcgggatg catccacctg
     9181 agaagcgctt caaaacttgc gatttcgcct gtcgacaact cgacctgggg ctggtagaaa
     9241 aggatgaatt cattgttcgc gagggcattc cggagcccgt tctccattgc catcctttcg
     9301 acgagttcat cgttcatttc ctgcctgaac atcatgtggg tgttctttcc ttcttttttc
     9361 gcatgatgca tggccacgtc ggagcttttc atcatttcct ctatggagtt cacctggtcg
     9421 ggatagacgc atataccgca gcttgccgat atatggaaag tattcctcat tacattgaac
     9481 ggttcattga atatgcccac tgctgtcgaa atcttttcat tcagttcatc cggcgaaccg
     9541 aaattcctga cgaaaacgac gaattcgtcc ccctcaaatc ttgcaaccag ctcgctttca
     9601 gtaaaagcat tcctgaggcg cttgccgatc tcaacgagaa gcatgtcccc gaccgaatgg
     9661 ctgaaagaat cgttgatgaa cttgaagttg tcgatatcca gatacaacag cgcgcacctt
     9721 tcgccttccc tgaacttgga aaacaccttt tccatttcat ttaataactc cgtcctgttg
     9781 gaaaggcccg tgagctgatc cttgaaggcc agctgccgaa ttttttcctc attggctgca
     9841 agctcgtcat acacgactgt cagttcctca aaactgtcct gcagtatgtt ttcctgcatg
     9901 accctgtcag ttatgtcaat accgattatc tcgataatat tggcattatc tttctcagta
     9961 atggcgctgc agtaaacgtg gacaactttg ctgtctcccc tgctgagcgc cagatcgaag
    10021 ctggtgctgc tgtcctgttc cctggtcgcg cccactgtgt tttcaagcat attccggtcc
    10081 gagtcggaaa tgagttcaaa aatattattt actttttctt cgccaaattt gtcaaaatac
    10141 agtaaaagat cctcggcata cctgttcgtc gtcagaatgg tgccgtcggt cctgatagtc
    10201 atggctatca ggtttgtaaa atcagcgatc ctatgggtgt taacctgctg tctctggaga
    10261 tcttccttca gcagggtcga acgtgacatt atgtatgcaa ttataaatga agctatcagg
    10321 atacacacca gaaggatcgc agacacaata ttgagcttcc tgaaaacatc gccctttggc
    10381 gccgtaacgg caatggacca accggtgctt tcgatcgcgg aaaagcccat ataccttttt
    10441 tcgccttcat aataatactc tcctgcgccc ttttctcctg cgatcattct tttttcaagc
    10501 tcgatgagct tgccgagttc aggtttgttt tccgaaatat gattgacacg cccatatacg
    10561 agcgcacggt ccctgtgggc gatgatatcg ccattcctat ttagtatgaa cgtgtaaccc
    10621 atgtcgctga atcttatgtt atcagcgatc ttactgatca catccgcatc ataagttaca
    10681 tacagcacac cggtcacttc accggattca aatacgggag aagcaaatac aatgacgatg
    10741 gcgccgtcgg cccggcttat tatcgggtcg gacacgctcg gtttcccttt cagcgcattc
    10801 tggaagtatt ccctgtcata gacattgagc gtgataccgt cggtcgatac tgcatttcca
    10861 tccaggtcag ctaaactaag ccttctgtaa ctgtcaagct tgagctgggc ccgcagttca
    10921 tcaaggcgct gttcaacagg caggctgctg tttctgatga tgctgttttc agccatggat
    10981 ctgacatcgg caaggcgccc atgaataaac tcactgatcg ttgcagcacc ctgttcggaa
    11041 aacttgacca gtgaattttc cacttcactt attaatatcc ttgtaactga atagtacaga
    11101 ataaaacctg cgattaatgc tacttccgca atctgcattg tttttgcgat caattggtat
    11161 ttattgccgt atttcagaat accacccctg ggaaacgctt tatattactg aaattgcctg
    11221 caggcatcat atgggatgta cctgcatagc aatattcctt tgggtcggac ccacaacata
    11281 gaagcaattt gccccgcacg cgaacaaatt gcttctatgc gcaagaagcc ttgttgctgt
    11341 ccacaaccgg ctcagcctct gagttctttc atacaggcag aacatatatt cttgcccctg
    11401 taattgacta catccctggc atcaccacag aatatgcatg ccggctcgta ctttttaagt
    11461 ataatattct gcccatctat aaaaatctca agtgcatctt tctcagctat atctaatgtc
    11521 ctcctcagct ctatgggcaa aaccactctg cccaactcat caactttcct tacaatacct
    11581 gtagacttca ttattatgtc acccccttgt acaattaata ttgtattaaa tcagtgaccg
    11641 aagccactgt tcgacc
//
LOCUS       DAOSCC010000054        11633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3710_length_11633_cov_4.696061, whole genome shotgun sequence.
ACCESSION   DAOSCC010000054 DAOSCC010000000
VERSION     DAOSCC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_11633_cov_4.696061"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLG72_04420"
     CDS             complement(<1..714)
                     /locus_tag="PLG72_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ80076.1"
                     /translation="MERYYDIYQQIAERTQGDIYIGVVGPVRTGKSTLIKRFMDLLVI
                     PNIENVYSRERAKDELPQSATGRTIMTTEPKFVPNEAVEITLDEALKLKVRMVDCVGY
                     LVRGAIGYLENNAPRMVSTPWFDYQIPFEQAAEIGTKKVINDHSTIGLLVTCDGTITD
                     IDREDYIEAERRVVNELKEINKPFVIVLNSVVPKDEETERLRQELEEKYEVPVISVNC
                     AQMQIEDIAAIMERILYEFP"
     gene            complement(853..1740)
                     /locus_tag="PLG72_04425"
     CDS             complement(853..1740)
                     /locus_tag="PLG72_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOJ80077.1"
                     /translation="MKKALIAYNPLSGNRNVPRELDYIVARFIRNDTLAVPFRLDPDN
                     SSELEKVLLNENFDYVVVSGGDGTVNSIASVLLSNNMDIPIGVIPAGTCNDLARSLNI
                     PTDMKKCLDTIFTGNITEIDTGFINGEKYFLNTCAGGVFVDVSYSTDSELKKRLGPLS
                     YYMTALGELANVRPIRLKLTADEEVVEGEFLLFLILNGRHAAGFWNLVEEADLSDGYM
                     DILLVKNCLHVEMAALFFKVLHNDFINDKNVMWLRTSKCRIEGDTGFNLSIDGEEWKG
                     LPIDVEFKHKSLKVFTPVK"
     gene            complement(1780..3249)
                     /locus_tag="PLG72_04430"
     CDS             complement(1780..3249)
                     /locus_tag="PLG72_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ80078.1"
                     /translation="MGQPAETSRVAVYARISRDDNKENFESIENQRDMLLAYTEANKL
                     GKVAAVYMDDNVSGSAVQRPGLDRMKEDILKGMIDVVLVKDLSRLGRNNAATLQLIDF
                     FEEHGVRVVTADGRFDSLVDSDMVGIETWVNERYVRDISRKIRAALRFKIQKGEYLGK
                     APFGYRKSGTEKNRLVADESEAHIVMLIYRLYLSGMGYTAISKYLEEKGYASPQNRGW
                     NRITVRRILSSRVYIGDTVQGVSEKVSFKSKKTRRLPESSWVITEGTHEALVPREIFD
                     KVQAMRMSRTGGRKVQGRMRHVLSGIIYCGGCGSAMYARRRSGGIAYVCGNYCRNGKK
                     ACTSHFVYEREIVGHICSELMSLFRLEDEIAKLKETFGDYKTDEQGRAWNDMNVRMEN
                     LLRRQELLYRDRLEGRISGQLFDRMNRQLEKHISALEDEMEKLRLEQTRSSDFSILVK
                     SITDRLEKGELTNEMVSAAVSSIVVHDETVTIDLKYKMA"
     gene            complement(3191..3403)
                     /locus_tag="PLG72_04435"
     CDS             complement(3191..3403)
                     /locus_tag="PLG72_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80079.1"
                     /translation="MKKRHASGRRFTIRHSGELTAGEVIVRLLKLAAGEGGQLAAGSQ
                     EGGRWRGDGTACGNQQSGGICQDKQG"
     gene            3550..3861
                     /locus_tag="PLG72_04440"
     CDS             3550..3861
                     /locus_tag="PLG72_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80080.1"
                     /translation="MARDIPNKLLTFLKTALDDVDDGYEYASELNRILNSDECRAALS
                     AREIDALRDYADEVKKVGEMDYYAKERISELEREHFGAGGIQGYLKGDRSEPEKPVWP
                     F"
     gene            complement(3920..5038)
                     /locus_tag="PLG72_04445"
     CDS             complement(3920..5038)
                     /locus_tag="PLG72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80081.1"
                     /translation="MEFAVDLNLVRSQKLLLTPQLKQAIEILEMDSRELFQYVDNQLD
                     TNPALEEAPAETTQYASAGIEATDSIDYCQYTEMADPVAEQPEKVLTLKQHLLVQLDA
                     LCLDKKSYAIGEYLIDNTDSNGYMKADTAEAAALLGVPESDVLKVLEKLQSFEPPGIC
                     ARNLVECLLIQLRQQDVRDRDAELIVEYFLDAVAGNDVACIANATGLSMERVNELFHK
                     IRSLEPKPGRNYRCNEMVSGTLPDVFIRDINGELLVVHNSEAFPNVCISEIYVSGLSR
                     RGAPRPGHVNEWLSSAVWLIKCLEQREDIIFTIAREICELRREFFKKGPRAFRHIDKR
                     SFASRVCLHETILDKALAGKYLQCRWGLYELRSFFKEA"
     gene            complement(5132..6073)
                     /locus_tag="PLG72_04450"
     CDS             complement(5132..6073)
                     /locus_tag="PLG72_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOJ80082.1"
                     /translation="MKNKLTLTGANILFFVFTVLLLGYQFIAAFMLGNEVYDSMYAVV
                     IINELLIAGSVLVYCIVKKIDLKETFRIRKLQLRPALLIILLSVPLFIAATMLNNLVV
                     YVLQFIAELPPQNLPIPKSPGELAVGILVIGLMPGVCEELMHRGFLLTAYERRGSYRA
                     VVFVSILFGLFHFDITNLLGPIFLGLVFGYYVVRTGSIFAGILAHFLNNAVAEVIQYL
                     WADPASVGTVSLTWQELLGIIGIGVPALAVAAVLLYLFRHATEGKTEIIPPIARPRQD
                     VKTVVSHWPVSAVLALYVIMLIINLFAMMAVKYFIPQ"
     gene            complement(6129..6710)
                     /locus_tag="PLG72_04455"
     CDS             complement(6129..6710)
                     /locus_tag="PLG72_04455"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ80083.1"
                     /translation="METKKEFRKYYMEKRSRLSKEEVMEKSALLFERVCSLDQYINAD
                     LVLAYMSFGNEVSTGPFIYRCIRDGKTAALPKVEKAAGMGCRLSIYGIKDPVDDVVPG
                     FKGILEPDPSKTRLLDTREPDIAIVPGVAFDVRGNRLGYGAGCYDRLLPLLREDCLKI
                     GVAFEIQLADSLPADHHDFRLDMIVTEKRTIMC"
     gene            complement(6784..7458)
                     /locus_tag="PLG72_04460"
     CDS             complement(6784..7458)
                     /locus_tag="PLG72_04460"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOJ80084.1"
                     /translation="MDLLLKEVFDLAGSLICHQLPERSLMAGDLVLPVCARDMGIYAG
                     IFTAFAYLAVMRRFGAQRPPGLAASVGMTVMMLPMMLDGVLSYAGIIETNNTVRLFTG
                     LFFGLPVPILLVPAANFSVYGRNDRPVLKNWTELIVVYVAGILICASILHGLMPYAAA
                     GLMYVAGLLSLISRIIFTVVRRSSTGSKWRIRIATVLGSASALIFLYVLSSYILQPLK
                     EMLLAR"
     gene            complement(7475..8002)
                     /locus_tag="PLG72_04465"
     CDS             complement(7475..8002)
                     /locus_tag="PLG72_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80085.1"
                     /translation="MEHNELKKCGFCGESIPANATRCPYCASILEIPVDDSYARAGDG
                     VTDDQGLDQEQQNGFSTGSDQQYPADRQPGYQDPNTSNRPYYAEHRQSMMQNGGKAPL
                     GNGLKVFLTLLFALLPGIGQLAGIITAIVFMSSSDDSDRRSFGSALLVANLILFILAC
                     LGCFVLALIGQRFAR"
     gene            complement(8104..9960)
                     /locus_tag="PLG72_04470"
     CDS             complement(8104..9960)
                     /locus_tag="PLG72_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOJ80086.1"
                     /translation="MKYRVDVHMDGNVRTAMADSGANLLEILNSMGISLYTPCGGRGT
                     CGKCIVRAEGGMAEPSENERLLLGERLKKGFRLACSFRIGSDVCIYIDSSGEQAVIMT
                     DGTRRQVALDPLVRKKYIELPLPSLEDQRPDMERVLELSGLPGMSAGTSISNLPGIET
                     ELELLKGLPALLRSSGYRVTTVGTGGKLLSVEAGDTTPRLYGAAFDIGTTTVAAYLYD
                     LNNGKLLGVASMMNPQRKFGADVISRIDYARTSGQAAEELRSLITECVNELTGRLADD
                     AGINVKDIYAAVFSGNTTMLHLLAGFDASGIAVSPFIPATCRTVRLGAKELGIDINGS
                     GHAILLPCVSAYIGADTVSAVLASGMYERDGFSLLVDIGTNGEIVLGGKDGLTACSAA
                     AGPAFEGANIRFGMGGVAGAVDSFAIQGFRYTVIGNIAARGICGSGIVDAAAALLDAG
                     AIDETGAIADDGEAAGLPQEIRKRFTATDGMRSFILVPAEETCIGEPITITQKDIREI
                     QNAKAAIAAGIETLISEAGISHDDIDKVFLAGGFGSSIHVRSAARIGLLPPQLTDRVA
                     AVGNASGSGASECLLSQEMLRIAEEIAGKVRYIELSSSAVFTEKYIENMMFA"
     gene            complement(9984..10412)
                     /locus_tag="PLG72_04475"
     CDS             complement(9984..10412)
                     /locus_tag="PLG72_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ80087.1"
                     /translation="MEVSVLDSITKKVSDTAKAAAKISGSVVEITKLNMSINAEEEKI
                     RKLYTEIGKQLYEDYTDGKAVSEELLRKCVKIDEIFENIAEMKDKILELRNVKACPNC
                     GTILDIEMEYCHKCGKKQEDIPEAVEENKDDKDSGKDGQD"
     gene            10661..>11633
                     /gene="gltX"
                     /locus_tag="PLG72_04480"
     CDS             10661..>11633
                     /gene="gltX"
                     /locus_tag="PLG72_04480"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ80088.1"
                     /translation="MAGVVRTRFAPSPTGYMHIGNLRTALYEYLIAKRYGGSFILRIE
                     DTDQERYVEGSTDVIYKTLKTAGLEHDEGPDKGGPFGPYIQSERKAIYMEYAKKLIDL
                     GHAYYCFCSKERLAALKEECERQGKQYKYDRHCLDLSREEIEQRLSEGVPFVIRQKMP
                     DSGTTSFEDAVYGTITVDNSELEDQILIKSDGLPTYNFANVIDDHLMGITHVVRGNEY
                     LSSTPKYNLLYRAFGWDVPVYVHVSTIITPEGRKMSKRLGDPSFEDLISQGFLPEAVV
                     NFVALLGWSPGTEQEIFSLKELEQVFDIRGISKSPAVFDMAKLRWMNG"
BASE COUNT         2706 a         3239 c         2662 g         3026 t
ORIGIN      
        1 cgggaattca taaaggatcc gttccattat cgcggctata tcttcgatct gcatctgggc
       61 gcagttcaca ctgatgacag gcacttcata cttctcttca agttcctgtc tcagcctctc
      121 tgtctcctca tctttcggca cgaccgagtt gagtacgatc acgaacggct tgttgatttc
      181 cttcagttca tttacgacgc gcctttccgc ctcgatataa tcttccctgt cgatatcagt
      241 tatagtcccg tcgcacgtca ccaggagccc gatggtcgag tggtcgttta tgaccttttt
      301 ggtccctatc tcggctgcct gctcaaacgg tatctggtag tcgaaccacg gcgttgagac
      361 cattctcggc gcattgttct caagatagcc gatcgccccc ctgaccaggt agccaacgca
      421 gtcgaccatc ctgaccttca gcttcaatgc ctcatccagg gttatttcaa ctgcctcatt
      481 aggaacgaac ttgggttctg tggtcatgat cgtacgtccg gtagcactct ggggcagttc
      541 atcctttgct ctttcacggc tgtatacgtt ctctatgttc ggtatgacga gcagatccat
      601 gaacctcttg atcaatgtcg atttacctgt ccttacagga cctacgacac ctatatagat
      661 atctccctgc gtcctttcag cgatctgctg gtatatatca taatatcttt ccacccgata
      721 acccccccta tatatggata tgcaaaagcc gggattcaca attaatatat gtccgaaaaa
      781 tgctattatt acaaaaagaa ggggtttgat cagctaaatc ggtgtggagt ttccgtcctg
      841 gcggcaggcc atttatttta caggagtgaa caccttaagg ctcttgtgtt tgaattccac
      901 gtctatgggc aggcctttcc actcctcgcc gtcgatgctg agattgaagc cggtatcgcc
      961 ttcgatgcgg catttgctcg tcctgagcca catcacgttc ttatcgttta taaagtcatt
     1021 gtggagcacc ttgaaaaaga gtgctgccat ctcgacatgc aggcagtttt taacaagcag
     1081 tatatccatg tatccgtcgg acagatcggc ttcctccacc aggttccaga atccggcggc
     1141 atgcctgccg ttcaggatca ggaacagcag gaactctcct tccaccactt cttcatctgc
     1201 ggtcagtttc agcctgatcg gtcttacatt tgcaagttcg cccaacgccg tcatatagta
     1261 ggacagcggg ccaagtctct tcttcagctc actgtcggtg ctgtaggaaa catcgacgaa
     1321 aacgcccccg gcgcaggtgt tgaggaagta cttctctccg ttgatgaacc cggtgtcgat
     1381 ttcggtaatg ttgccggtaa aaatagtatc aaggcacttt ttcatgtcgg tcggtatatt
     1441 caggctgcgg gccagatcat tgcacgtccc tgccggtatg accccgatgg ggatatccat
     1501 attgttgctg agaagtactg atgcgataga gttgactgtt ccgtcgccac ccgatacgac
     1561 aacataatca aaattctcgt taagcaaaac cttttccagt tcggacgagt tgtccgggtc
     1621 cagtctgaag ggaacggcaa gcgtatcgtt ccggatgaaa cgggcaacga tatagtccag
     1681 ttctcttggt acgtttctgt ttcctgaaag gggattgtaa gcgatcaaag cctttttcat
     1741 aatacagcct ccaacgaagt actctgaaca aaatttatct tacgccattt tatattttaa
     1801 atcaatcgtc acggtctcat catgcactac gatcgaactg acggccgccg aaaccatttc
     1861 gttcgtcagt tctccctttt ccagcctgtc cgtgatgctt ttcaccaaaa tactgaaatc
     1921 ggatgaccgt gtttgttcca gtctcagctt ttccatctca tcctccaatg cgctgatatg
     1981 cttttccaat tgccggttca tcctgtcgaa caactgaccg gagatccttc cctccagtct
     2041 gtccctgtag agcagttcct ggcggcggag caggttctcc atccgtacat tcatatcgtt
     2101 ccaggcacgg ccttgttcat cggttttgta atctccaaac gtttctttca gctttgcgat
     2161 ctcatcctcc agcctgaaca ggctcatcag ttcgctgcag atatgcccga cgatctcccg
     2221 ttcatatacg aaatggctgg tacacgcttt ttttccgttc ctgcagtaat tgccgcagac
     2281 atatgcgatc ccgccgcttc tcctgcgcgc atacatcgca cttccgcagc ctccgcagta
     2341 tatgatcccg ctgagcacgt gccgcatcct gccctgcact ttcctgcctc cggtcctgct
     2401 catccgcatt gcctggacct tgtcgaaaat ctccctgggc accagcgctt catgggtccc
     2461 ttccgtgatg acccagctgc tctccggaag ccgccttgtc tttttgcttt tgaaactcac
     2521 cttttcactg actccctgta ccgtgtcacc tatatatacg cgggaactca gtatacgtct
     2581 gaccgttatc ctgttccagc ccctgttctg tggagaggca taacctttct cttcaaggta
     2641 tttagatatg gcggtatagc ccatccctga aaggtacagc ctgtaaatca gcattacgat
     2701 atgggcttcc gattcatccg ccaccaacct gtttttttcg gtcccggact tcctgtaacc
     2761 aaagggggcc ttcccgagat actctccttt ctggatcttg aacctgagag cggcccggat
     2821 ctttctcgat atatccctta catacctttc atttacccat gtttctatgc cgaccatatc
     2881 gctgtcaacc aggctgtcga accgtccgtc ggcagtaacg accctgacgc catgttcctc
     2941 aaagaaatct atcaactgaa gcgtcgcagc attgttcctg ccaagacggg acaggtcctt
     3001 gaccagcacc acatcgatca tgcctttaag tatatcctct ttcatcctgt ccagccccgg
     3061 cctttgcact gccgaaccgg atacgttgtc atccatgtag acagcggcca cttttccaag
     3121 cttgtttgct tctgtgtagg ccagcagcat atcgcgctgg ttttcgatgg attcgaaatt
     3181 ttccttattg tcatccctgc ttatcctggc atataccgcc actctgctgg tttccgcagg
     3241 ctgtcccatc tccgcgccac ctcccgcctt cctgtgatcc ggcagccaac tgcccgcctt
     3301 cgcccgcagc gagtttaagg agccttacga tgacctcccc tgcggtcagt tcgcctgaat
     3361 gcctgatggt aaatctcctt ccgctggcat gtcttttttt caaccgatcc tccggcatac
     3421 tatgaccaaa actctggtat atatatattt cagatatttt gtcctttatc agggctattt
     3481 tcatacagat atacctgtcc gtacaggaaa gctgtataat atatctgata gacagggaag
     3541 ggtgatggta tggcaagaga tattccaaac aagctgttaa cattccttaa gaccgcattg
     3601 gatgatgtgg atgacggata cgaatacgca tctgaactga acaggatcct taactcggat
     3661 gaatgccggg ctgctctttc tgccagggaa atagacgcac tgcgtgatta tgctgatgaa
     3721 gtaaaaaaag tgggagaaat ggactattac gccaaagaga ggatatcgga actggaacgg
     3781 gagcatttcg gcgcaggagg tatacagggt tatctgaaag gtgaccgcag cgagcctgaa
     3841 aagcctgtct ggccgtttta acatccgata atgggaaagg ctgcccgaca ccaggcagcc
     3901 tttcctgccg atatatcgtt catgcttcct tgaagaagct cctcagttca tacagtcccc
     3961 acctgcactg caggtatttg cctgcaaggg ctttgtccag tattgtttcg tgcaggcata
     4021 cccgtgatgc gaacgatctc ttgtcgatgt gcctgaatgc cctgggccct tttttgaaga
     4081 attccctcct cagttcgcat atttcccttg cgatcgtgaa tatgatgtcc tccctctgtt
     4141 ccaggcactt gatcagccat acggcactgc tgagccattc attcacatgc cctggcctcg
     4201 gagcgcccct gcgggacagc cctgacacat agatctcgga tatgcagaca ttggggaatg
     4261 cctccgaatt gtgaacgacc aggagttccc cgtttatatc cctgatgaag acatccggca
     4321 atgttccgct taccatctca ttgcagcgat aattccggcc cggtttgggc tccaggctcc
     4381 tgatcttatg gaagagttca tttacacgct ccatgctcaa accggtggca ttggcgatac
     4441 aggccacatc gttgcctgca actgcatcca ggaagtattc gactatcagt tccgcatccc
     4501 tgtcacgtac gtcttgctgt ctcagttgga taagcagaca ctcgacgagg ttccgtgcac
     4561 atatgcccgg cggttcgaaa gactggagtt tctcaagcac tttcagcaca tcgctttccg
     4621 gtacgccgag aagtgccgcg gcttctgcag tgtcggcctt catatagccg ttgctgtctg
     4681 tgttgtcgat caggtattct cctatggcgt aactcttttt atccaggcaa agcgcgtcaa
     4741 gctggaccag aagatgctgc ttgagcgtca gtaccttttc aggctgttcg gccacaggat
     4801 cggccatttc agtatattga caataatcaa tggagtctgt tgcttcgata ccggctgaag
     4861 catactgtgt tgtttctgcc ggcgcttctt cgagagcggg gttcgtatcc agctggttat
     4921 cgacgtattg gaagagttcc cgtgaatcca tttcgagtat ttcgatcgcc tgcttcaact
     4981 gaggtgtaag caaaagcttt tgtgaacgca caagattcag atcgacggcg aattccatac
     5041 gggccacctc aaaaaaaggt tatatagtat tatataacct cagaggctcc tttagtaatg
     5101 ctggcaggct ataaaaacaa aacacgtgat tttactgcgg gatgaaatac ttcaccgcca
     5161 tcatggcgaa caggtttatt atcagcataa tgacatataa agcaagcact gccgaaacag
     5221 gccaatgcga caccactgtc ttcacatcct ggcgaggtct tgcgatcggc gggatgattt
     5281 ccgttttgcc ttcagtggca tgcctgaata aatacaggag caccgcggca acggcaagtg
     5341 caggaacgcc tattccgatg atcccgagca gttcctgcca tgtcagagac acggtcccga
     5401 cggaagcagg atcagcccac aggtactgga tcacttccgc caccgcattg ttgagaaaat
     5461 gggcaagtat acctgcgaat atcgaaccgg tcctgactac ataatagcca aataccaagc
     5521 caaggaagat cggtccgagc aggttcgtga tatcgaagtg gaacaggccg aaaagtatcg
     5581 acacgaagac cactgccctg taagaaccac gcctttcata cgctgtcagc aggaatccgc
     5641 ggtgcatcag ttcctcacat actcccggca tcaaacctat gaccaggata ccgacagcaa
     5701 gttctcccgg gcttttgggt atcggcaggt tttgcggcgg cagttcggca atgaactgca
     5761 ggacatatac gacaaggttg ttcagcatcg ttgctgcaat gaacaggggt acggacagca
     5821 gtataatgag cagtgcgggt ctcaattgca gttttctgat cctgaaggtt tccttaagat
     5881 cgattttctt cacgatgcag taaaccagta cagagccggc aataagcaat tcgtttatga
     5941 taacgacggc atacatgctg tcatagactt cgtttcccag catgaatgcg gcaatgaact
     6001 ggtatcccag caataagacc gtgaaaacga aaaacagtat gttcgcaccc gtaagtgtca
     6061 gtttgttctt catctgatta acccttccct tatattttta taaacaataa tatatgcctt
     6121 acgtatgttc agcacattat tgtccttttc tccgtaacta tcatgtcaag tctgaagtca
     6181 tgatgatccg ccggcagact atctgccagc tggatctcaa aagcaacgcc tattttcagg
     6241 caatcctcac gcagcagcgg aagcagcctg tcataacagc cggcgccgta accaagcctg
     6301 ttcccacgga catcaaaggc aactccgggc acgattgcaa tatccggttc acgcgtatcc
     6361 agaagccgcg tttttgacgg atcgggttcg agtatgcctt tgaagcccgg caccacatca
     6421 tcaacgggat cttttatacc atatatgctc agcctgcatc ccatcccggc cgctttttca
     6481 actttcggca gcgccgctgt ttttccgtcc ctgatgcatc tgtaaataaa tggtccggtg
     6541 gacacttcgt ttccaaatga catataggcc agaaccaaat ccgcatttat atactgatcg
     6601 agagaacata cccgttcgaa cagcagtgcg cttttttcca tgacctcttc tttgctgagt
     6661 cggcttcgtt tctccatgta atacttccga aattcctttt ttgtctccat aaccgtcccc
     6721 cacctttgaa gaccggcagt cacacttcct gcagcactga tgccttcatc ggcaatcgcc
     6781 gtttcatcgg gcgagcagca tctccttcaa aggctgcagt atgtacgaag aaagaacata
     6841 taaaaatata agcgccgatg cgctccccaa aacagtcgcg atgcggatcc tccacttgct
     6901 tccggtactg cttctgcgga ccacggtgaa aatgatcctt gatatcagcg acaaaaggcc
     6961 ggccacatac atgagaccag cagcagcgta aggcatcaga ccatgcagga tcgatgcgca
     7021 gatcagtatc cctgccacgt atacaactat cagttcggtc cagtttttca aaaccggcct
     7081 gtcattacgt ccgtatacgc tgaaattggc ggcaggcacg agcagtatcg ggacaggcag
     7141 gccaaaaaac agtcccgtga acagtctcac agtgttgttc gtttcaatga tgcctgcata
     7201 cgacagtacg ccgtcaagca tcatcggaag catcatcacg gtcatgccca ctgacgcggc
     7261 cagtccggga ggcctttgcg cgccaaacct gcgcatgaca gccaggtatg cgaaagctgt
     7321 gaatatcccc gcatatatcc ccatatcgcg cgcacacacg ggaagtacca agtctccggc
     7381 catcagcgac ctctcgggga gctgatggca gatgagcgat cctgccaaat caaaaacttc
     7441 cttcaacagc agatccattt ccataataga tccgttatct ggcaaatctc tgaccgataa
     7501 gcgccagtac gaaacaaccc agacatgcca aaatgaacag gatgagattc gccacaagaa
     7561 gcgccgaccc gaaagaacgt ctgtcgctgt catcgctgga actcatgaaa acaatggctg
     7621 ttatgatgcc tgcaagctgg cctatgcctg gcagcaatgc gaaaagcagg gtcaggaata
     7681 ccttaagccc gttgccgaga ggggctttcc cgccgttttg catcatactc tgccggtgct
     7741 cggcgtaata cggcctgttt gaggtattgg gatcctggta tccgggctgc ctgtcggcgg
     7801 ggtattgctg atccgaacct gtactgaagc cgttttgctg ctcctgatcc agcccctggt
     7861 catcggtcac gccatcgccg gcccgggcgt aactgtcatc gacaggtatt tcaagtatgc
     7921 ttgcacagta tggacaacgc gttgcgttgg caggtatgct ctcaccgcaa aaaccacatt
     7981 ttttcagttc attatgttcc aaaagtctgc ctcctcacta tagatagttt tgaaactatt
     8041 cttttactat tctctcagca accggaaaaa ccctttttcc acctgcaaaa attcgtccgg
     8101 gttctatgcg aacatcatgt tttctatgta tttttcggta aaaacggcag atgacgacag
     8161 ttctatatat ctgactttcc ctgcgatctc ctccgcaatg cggagcattt cctgcgataa
     8221 aaggcattcc gacgctcccg aacctgacgc attgccgaca gctgcgaccc tgtccgtaag
     8281 ctgcggcggc agcaggccta tccttgccgc actccgtaca tgtatgctgc tgccgaaccc
     8341 cccggccagg aaaactttgt cgatatcgtc atggctgatc ccggcttcgc ttatcagggt
     8401 ttcgatcccg gcggcaatgg cagccttggc gttctggatc tcgcgtatat ccttttgtgt
     8461 gatggttatc ggttcaccga tgcaggtctc ctctgccggg accaggatga acgatctcat
     8521 accgtccgtc gcggtgaacc ttttccttat ttcctgcggc aggccggccg cttcgccgtc
     8581 atcggcgatc gctcctgttt catcgatggc tccggcgtca aggagcgcag cggcagcatc
     8641 aactattccg gatccgcaaa tgcctcttgc tgctatatta cctataacgg tatacctgaa
     8701 tccctgtatc gcaaatgaat cgacagcgcc ggcaaccccg cccatgccga accttatgtt
     8761 cgctccttcg aacgcaggac cggctgctgc ggaacatgcg gtgaggccgt cctttccgcc
     8821 gaggactatc tctccgttgg ttccaatatc cacaagcagc gaaaaaccgt ccctctcgta
     8881 cataccgctg gcaagtacgg cagataccgt atcggctcct atatatgcgg aaacgcacgg
     8941 aagcagtatg gcatggccgc tgccattaat atcgatgcca agttccttcg cgccaaggcg
     9001 cacagtcctg catgtggccg gtatgaacgg cgacactgct ataccggagg cgtcgaatcc
     9061 ggcaaggaga tgcagcatgg tcgtgttgcc gctgaatacg gcggcatata tgtcttttac
     9121 gttgattccc gcatcatcgg caagacggcc cgtcagttca ttcacacatt ccgttatcag
     9181 gctcctcagt tcttccgcag cctgccctga agttctggca tagtcgatcc tcgaaatcac
     9241 atccgcaccg aatttgcgct gaggattcat catcgaagcc acgccgagca gttttccgtt
     9301 gttcaggtca tacagatacg cagctacggt ggtcgtacca atgtcaaaag cggcgccgta
     9361 aagccgcggc gtagtatcgc cggcttcgac agataaaagt ttcccgccgg tcccgacagt
     9421 cgtgaccctg tatcccgaac ttctcaataa tgccggcagc ccttttaaca actcaagttc
     9481 cgtttcaatt cccggcaggt ttgaaatgct tgtcccggcg ctcataccgg gcaggccgga
     9541 caactccagt accctttcca tgtccggcct ctggtcctcc agtgacggaa gaggcaattc
     9601 gatatatttt ttcctgacca gcgggtccaa agcgacctgt ctccgggtac cgtccgtcat
     9661 aatgacagcc tgttccccgg aactgtcgat atatatgcat acatcggaac cgatcctgaa
     9721 actgcatgcc agcctgaaac cctttttaag cctttctccc agcagcagcc tctcgttttc
     9781 ggaaggctcc gccataccgc cttccgccct tacaatgcat ttcccgcatg tgcccctgcc
     9841 tccgcacggc gtatagagag atatccccat actattcagg atctccagga gattggctcc
     9901 gctgtccgcc atagcagtcc ggacgtttcc atccatatgt acgtcgaccc tgtatttcat
     9961 cacagcctcc aaaaccgccc gcctcagtcc tgtccgtctt tgccgctgtc tttatcgtct
    10021 ttgttttcct ctactgcttc cggaatatct tcctgtttct tgccgcactt atggcagtat
    10081 tccatttcga tgtcgagaat ggtgccgcag ttggggcaag ccttcacgtt cctcagttca
    10141 agtatcttat ccttcatttc tgctatgttt tcgaatatct cgtctatctt tacgcacttt
    10201 ctcagcagtt cctcgctgac cgcttttcca tccgtataat cttcataaag ctgtttgcct
    10261 atctccgtgt ataacttcct gatcttctcc tcttctgcat taatgctcat gttgagcttc
    10321 gtgatctcca cgacactgcc cgaaatcttt gctgcagcct tcgccgtatc ggacaccttc
    10381 tttgtgatac tgtcgagaac agacacttcc atctctcctt tcagaagctt actggtactg
    10441 cggtttactg cgcagacaaa ggtgggtgct cctaccgctt tgtcactata ttataatatt
    10501 ccaacgggac tgcaaataca ataacaaatc acatcccggc ggacgcaggc gttaccagcg
    10561 cgaatatgcc tgttccttga ctaacataat agtaaataat ataatctaag ggaaaaatca
    10621 gcgaaacctt tattcttact gcgagaggag agaccggcaa atggcgggag tcgtaaggac
    10681 gcggttcgca ccaagtccga caggttacat gcatataggc aatcttcgca ctgccctgta
    10741 cgaatatctc atagcaaaaa ggtacggagg cagtttcata ctcaggatag aagatacgga
    10801 ccaggaaagg tatgtagaag gttcgaccga tgtgatatat aaaacgctca aaaccgcagg
    10861 gcttgagcat gatgagggcc ctgacaaggg cggtccgttc ggtccatata tccagagcga
    10921 acgtaaagcg atatacatgg aatatgccaa aaagctgata gatctcggcc acgcctatta
    10981 ctgcttctgc tcaaaggaaa ggctggctgc gcttaaagag gaatgcgagc ggcagggaaa
    11041 gcagtacaaa tacgacaggc actgccttga cctctcacgt gaggaaatag aacagagact
    11101 ttccgaaggc gttccgttcg tcatccgcca gaagatgccc gactccggga ccaccagctt
    11161 cgaggatgcg gtatacggga ccataacggt cgacaacagc gagcttgagg accagatact
    11221 catcaaatcc gacgggcttc ccacatataa ttttgccaac gttatcgacg accatcttat
    11281 ggggataact catgtggtaa ggggtaatga gtacctgtct tcgacgccca agtacaacct
    11341 gctttacagg gctttcggat gggatgtgcc tgtatatgtg cacgtttcca ccatcatcac
    11401 ccctgaaggc cgcaaaatgt caaaaaggct tggcgatcct tccttcgagg accttatcag
    11461 ccagggattc ctgcctgaag cagtggtcaa cttcgttgca ctgcttggat ggagcccggg
    11521 cacggagcag gagatcttct ctctgaagga actcgagcag gtctttgata tccgcggcat
    11581 aagcaaatct ccggccgttt tcgacatggc aaagctgaga tggatgaacg gcg
//
LOCUS       DAOSCC010000055        11575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3726_length_11575_cov_4.320573, whole genome shotgun sequence.
ACCESSION   DAOSCC010000055 DAOSCC010000000
VERSION     DAOSCC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_11575_cov_4.320573"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLG72_04485"
     CDS             <1..634
                     /locus_tag="PLG72_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ80089.1"
                     /translation="RKNSYTIKDLKRLGMLDDELEALLRRLAASDASVLFCGKGADGK
                     TTLMRAFINILPEMERVLIVESDAEIYPDKPYCIEQRIKKKNEGGRPVTLKDLVLDGL
                     TMSLDTYCVGEITGDEAWEFVKAAFSGHRGIATTHSESAEDAFGRLLTLSKGANIGES
                     EKTIKEVLGRSIDVIFYLKEFRVVEVLEVLGYDGQTDRFVYKRLYPADRG"
     gene            640..1617
                     /locus_tag="PLG72_04490"
     CDS             640..1617
                     /locus_tag="PLG72_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80090.1"
                     /translation="MLVIISVLLAAADTVFAVMAARSLSRANAVERLKNWFRQKLPGR
                     NTHISPGSRHRSGPGAYDPVWKDRLNLLERIELLYIEKSNIRRYVPFMNIYILMLLVL
                     LIFVSSYRYVYGILRFVPSAVVISGILSAAPLFLLELLARYNTESIRKKLSEYISVLN
                     RWCSVKENIMYAFEKSLDSNIGEPLHTFIRDMVIQVNRGMDPCEALDMLQMKVDDAQF
                     SDFIVNIKLSIRHRGDIRKLLTNLENQFYKIDEEYNRRRISTYRDRVVICTVMFAVLP
                     LSYFFIKMSPKVGNFYLETLSGKILLMVFSVMYAFGFYLTAGITRFRSR"
     gene            1614..2540
                     /locus_tag="PLG72_04495"
     CDS             1614..2540
                     /locus_tag="PLG72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ80091.1"
                     /translation="MKEGDIVLDHFAVLYRENRIMFLLLGAINVVLGVLLSRGVSLYG
                     SGRVTEAMGARKPAVSPYRRCSMLVEQSLVACGKLIEKRSIYQKARDKMKKAGYRGEY
                     APVAYIFVRYVVSAVIFLAALFTNYPDVIRPIAFVVLVNSIVSAVISANKRKVNLRFQ
                     KHIYKIYKYLHNQISSGVKPTDAVRTVYETTDDREIRDVLIRLAARYELTLDIDSALE
                     ELRSSFDTHEAETLCLALKQGIQTGDNKELLAKQEDIMFKKYFNYIQAETDSCRNRGV
                     AAAAVFVAIIAVMIIVPMLDDLGQAVGRIFIN"
     assembly_gap    2648..2747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2748..3083
                     /locus_tag="PLG72_04500"
     CDS             <2748..3083
                     /locus_tag="PLG72_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80092.1"
                     /translation="VVFLLVYMVEFFIPLSVKADIDMICRNALLNMENAGGLSSEKKH
                     ELKTELESKGLTGVVITATENVKQGEKLTLRVEGEYTYSRITSLFGRGSETLRIVYDK
                     STMSRKVVN"
     gene            3089..3616
                     /locus_tag="PLG72_04505"
     CDS             3089..3616
                     /locus_tag="PLG72_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80093.1"
                     /translation="MKGRILEKGSAVTQIVLMAAMLVFIIIPVFSAVIEKYILMEKAR
                     VIRDSLDMTNISAYNALSSTDLGKVGIGAERSEVLEIYRQILSANLRLHDDLEPKPDS
                     VAEGRVEVLSLEIYSSGFPAQCPEGATVTRPTVHSSICVPVRPSLYRSIILKMLGRDY
                     VDIIVHTDTEIPVNN"
     gene            3643..4326
                     /locus_tag="PLG72_04510"
     CDS             3643..4326
                     /locus_tag="PLG72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOJ80094.1"
                     /translation="MRRYGWICGAFVIIILLVVIEMKIISNASGYEAKERVVVAARDI
                     GEDTVITGEMLEIREISSGAVHGDAVRKIEDAVGMTAADPIYDGEVLLKGRLTEEASD
                     TVEALNKSNRLYCIEPEWDQANAWQLEKDQQVDVVFVPNHLNQDNACPEASGVVSVTP
                     SQTGMKILKNIRIAGLVDEDGVIMKNCETEKIPKYIILEVTQDQAVFLAYAKSNGRIE
                     LLAIPGRKP"
     gene            4549..4785
                     /locus_tag="PLG72_04515"
     CDS             4549..4785
                     /locus_tag="PLG72_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOJ80095.1"
                     /translation="MAELKYEIRKFLGVIGEGTKGWKKEVNLISWNDRKPKLDIRDWD
                     ENHEKMGKGITLSRSEVEELKRLLAAFDPGEMEA"
     gene            4923..6251
                     /locus_tag="PLG72_04520"
     CDS             4923..6251
                     /locus_tag="PLG72_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80096.1"
                     /translation="MKTKHIVLLMILLTFIAMAGCMNGESAGNGGGPVKPASSDGGSE
                     PAIASDSSQAAVTEPAGQTGAGPVGKDGDRGVVEITGNYTRFDGIKGFDGDYQYLSMV
                     GETDGHIVMQYMPVEYSKEVKPSLMLVDIDTLEIARNVKLYNVLPEIGENPVSHTRVA
                     GDRIIVCLESLVAVFDESLELIQEIPLPEAIVRKAGRTVTYDKNGIIEVFYGGYDISD
                     DLGKIVYTDETGIWLYDLGSGTEELLAENIYNEDYLKRSFYRFPRFIADSGKIIADRL
                     GYEYSLGYAVLSISGKSLQTLDLFSERGMNDIYYDTGLLEPETNMLNKETGDYERKSF
                     YLDFETGNVTEIQIEKSIYDRYPFRDNFYVGENYAVFVTYIWADDGDYYETRDDMFYL
                     NRIDLNTLEMETDLLSVRAGGPHILGVLADGRILFRYYYDETDNGVCIAR"
     gene            6431..7318
                     /locus_tag="PLG72_04525"
     CDS             6431..7318
                     /locus_tag="PLG72_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80097.1"
                     /translation="MDRLLPKAWEGLLNSSNFLPVIVGTLERIQDATWSMEPNRHDNY
                     EMVYMKKGYAVFEISGHKVELGPNDIIIIKPMQYHKFIVKSESGCEFIVLYFTFENRI
                     NGEYAQIPLEDFLNFVSNKESSPYIKLKVSPKNEIVVLLNRIMKERESSEPGSEFLNY
                     LLIMELFVLLSRALKMEWENSIKLKSSKLKELIGISINYIHNNFERDISLGDIAKYVF
                     LSPSYFTRAFKEETGMSPISYLLKVRIERAKELLVDTSLKISDIALNVGFSNQQRFNE
                     MFKKYAGLTPLQYRKKNAR"
     gene            7663..8946
                     /gene="leuC"
                     /locus_tag="PLG72_04530"
     CDS             7663..8946
                     /gene="leuC"
                     /locus_tag="PLG72_04530"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOJ80098.1"
                     /translation="MADTGTKARGMTMTQKILADHAGLDRVEAGQLIRARLDMVLGND
                     ITTPVAVKEFRKIGRDRVFDKNKIAIVPDHFTPNKDIKSAEQVRFIREFAREMGIVNF
                     FEVGRMGVEHALLPEMGLVVPGDVVIGADSHTCTYGALGAFSTGIGSTDMAAGMATGE
                     AWFKIPEAIRFVLTGRPGKWVSGKDIILHIIGMIGVDGALYKSMEFTGDGLGHLSMDD
                     RFSMANMAIEAGAKNGIFDVDEKTVEYVKEHSTKSYKIYKADEDAVYCCEYVIDLGTV
                     KPTVAFPHLPSNARTIDKVGSIKIDQVVIGSCTNGRIEDMRTAAQVLKGRTVHPDVRC
                     IIIPATQKVWKQAMDEGLFDIFIEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRN
                     FVGRMGHPESEVYLASPAIAAASAVAGRIASPDEL"
     gene            9052..9540
                     /gene="leuD"
                     /locus_tag="PLG72_04535"
     CDS             9052..9540
                     /gene="leuD"
                     /locus_tag="PLG72_04535"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOJ80099.1"
                     /translation="MKVHGNVIKYGDNVDTDVIIPARYLNTSEPKELAAHCMEDLDPG
                     FAGKVKAGDIIVAGKNFGCGSSREHAPIAIKASGISCVIAKTFARIFYRNAINIGLPI
                     VECPEAAEDIGDGDVVEVDFDTGTITNKTKNKSYKAVPFPGFMQEIIKADGLVGYIRN
                     QG"
     gene            9698..10786
                     /gene="leuB"
                     /locus_tag="PLG72_04540"
     CDS             9698..10786
                     /gene="leuB"
                     /locus_tag="PLG72_04540"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOJ80100.1"
                     /translation="MNYRIAVIKGDGIGPEIVDQAIAVLDAISEKCGISFTFSEVLMG
                     GCAIDEYGVPLPESTLETCKESDSVLLGAVGGPKWEGLPGDRTPEAGLLGIRAGLGLY
                     ANLRPAVIYEALREASPLKPEIVGGKIDILVVRELTGGIYFGERGRNGKGTPDEWAYD
                     TERYSVPEIERIARIAFEAASKRRGIVTSVDKANVLESSRLWREVVTRVSRDYPGVAL
                     NHMYVDNAAMQLVRNPGQFDVIVTTNMFGDILSDEASMITGSIGMLPSASLGSGKPGL
                     YEPIHGSAPDIAGQDKANPIATILSAAMMLRYSLGENAAADLIENAVVKVLEKGYRTP
                     DIASFGGTVTGTKEMGRLIAEEVRRAEP"
     gene            11369..>11575
                     /locus_tag="PLG72_04545"
     CDS             11369..>11575
                     /locus_tag="PLG72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80101.1"
                     /translation="MGKYIVKRILLAIVTLWLVATITFILMNMVPGGPFLAEKAPSEA
                     TLKALNEKYGLDKPLIVQYKNYLTR"
BASE COUNT         3207 a         2454 c         3213 g         2601 t
ORIGIN      
        1 caggaaaaac agctacacga taaaggacct gaaaaggctc gggatgcttg acgatgaact
       61 tgaggcactg ctcaggagac tggcggccag tgacgcctct gtgctgttct gcggcaaggg
      121 ggcggacgga aagaccacgc tgatgcgcgc tttcatcaac atactgccgg agatggagag
      181 ggtgctcatc gtggaatccg acgccgagat ctatccggac aaaccctact gtatcgaaca
      241 gaggatcaaa aagaagaacg agggcggaag gcctgtgact ctgaaagacc tggtcctgga
      301 cggtttgacc atgagcctgg atacatattg tgtaggggag ataaccgggg acgaggcatg
      361 ggaatttgtc aaggcggctt tctcaggaca caggggtatc gcgactaccc attcggaaag
      421 cgccgaagat gcattcggac ggctgctgac gcttagcaag ggagccaaca taggcgaaag
      481 cgagaagacg ataaaggaag tgcttggaag gagcatagac gtcattttct accttaagga
      541 attccgcgtc gtggaagtcc ttgaagtact cggatacgac gggcagactg acaggttcgt
      601 ctataaaagg ctgtaccccg cagacagggg gtgatgctga tgctggttat catatctgtt
      661 ttactggctg cagctgatac ggtgttcgca gtgatggcag caagaagcct cagccgggca
      721 aatgccgtgg aaaggctgaa gaactggttc cggcagaagc tgcctggaag aaatacccat
      781 atttctcccg gcagccggca caggtcaggt cccggcgcat atgacccggt atggaaggac
      841 cgtttgaacc tgcttgagcg gatcgaactg ctatacattg agaaatcaaa tataaggcgc
      901 tacgtacctt ttatgaacat ttacatccta atgctcctgg ttttgctgat atttgtttcg
      961 tcttaccggt atgtttacgg gatattgaga tttgtccctt cggcagtcgt gataagcggg
     1021 atcctgtccg cggctccgct gttcctgctg gaactgctgg caaggtacaa tacggaaagt
     1081 ataaggaaaa agctgtctga gtacatatcg gtgctcaaca gatggtgctc ggtcaaggaa
     1141 aacataatgt atgcgttcga aaagtcgctt gattccaaca tcggggagcc gctgcacaca
     1201 ttcataaggg atatggtcat acaggtgaac cgcggtatgg acccgtgcga agcgctggac
     1261 atgctgcaga tgaaggtgga tgatgcacag ttcagtgatt tcatcgtaaa catcaagctt
     1321 agcatcaggc acaggggcga tatcaggaag ctgcttacga acctggaaaa ccagttctac
     1381 aaaatcgacg aggaatacaa caggcgcagg atatcgacgt acagggaccg tgtagtgatc
     1441 tgtaccgtca tgtttgcagt attgccttta tcatacttct tcataaaaat gtcgccgaag
     1501 gtcgggaact tttaccttga aacactcagc gggaagatac tgctgatggt attcagcgta
     1561 atgtatgcgt tcgggtttta cctgaccgct ggcataacca ggttcaggag ccggtgaagg
     1621 aaggtgatat cgtgctggat cattttgcgg ttttatatcg ggagaacagg atcatgttct
     1681 tactgcttgg agcaattaat gtggttcttg gtgtgttgct ttcaagggga gtttccctgt
     1741 acggatcggg acgggtaacg gaagccatgg gagcgagaaa gccggccgtt tccccataca
     1801 ggcggtgttc gatgctggtg gaacagagcc ttgttgcctg cgggaaattg atcgaaaaaa
     1861 ggagcatata ccagaaggca agggacaaaa tgaagaaagc cggttacaga ggtgaatacg
     1921 cccctgtcgc atacattttc gtcaggtatg tggtttctgc agtgatattt ctggcagccc
     1981 tgtttaccaa ctatcctgac gtcataagac ccatagcctt tgttgtcctg gtcaattcca
     2041 tcgtttcggc agtgatatcc gccaacaagc gcaaagtgaa cctgcgtttc cagaagcata
     2101 tatacaagat atacaaatac ctgcacaatc agatctcctc aggtgtgaag ccgactgatg
     2161 ccgtaaggac cgtatatgag actacggatg accgggagat cagggatgtg cttataaggc
     2221 tggcggcgag atatgaactc acgctggata tcgacagtgc cctggaagaa cttcgttcaa
     2281 gctttgacac ccatgaagcc gagacgctgt gccttgccct gaaacagggg atccagaccg
     2341 gcgacaacaa ggaactgctt gcaaagcagg aagatatcat gttcaagaaa tacttcaact
     2401 acatacaggc cgagaccgac agctgcagga acaggggcgt tgcggccgca gctgtgttcg
     2461 ttgccataat tgccgtgatg ataatagtcc cgatgcttga cgatttgggt caggctgtgg
     2521 gcaggatatt tataaactaa gcgaacggag ggtgcaaaat gaaacttgtg aaactggtca
     2581 gggaaaagtt catcgacagg caggggggat ttggcctcaa tgagatactt ggcatagcag
     2641 cggcactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt gtttttctgc
     2761 ttgtgtacat ggtggagttt ttcattcccc tgtcagtcaa agccgatatc gacatgatct
     2821 gcaggaatgc tttactgaac atggagaatg ccgggggact gagcagtgag aaaaagcatg
     2881 aactgaaaac tgaactcgag agcaaagggc tgaccggagt ggttattaca gccactgaaa
     2941 acgtaaaaca gggcgagaag ctgacactgc gcgttgaggg cgaatatacc tacagcagga
     3001 taacttcact ttttggcagg ggcagtgaga ctctgcggat agtgtatgat aagtcgacca
     3061 tgtcgcgaaa ggtggtcaac tgaggtaaat gaagggacgg atcctggaaa aaggcagcgc
     3121 agtcacgcag atcgtattga tggcagcgat gctggttttt attataatac cggtcttttc
     3181 ggcagtcatc gagaaataca tactgatgga aaaggcgaga gtcatcaggg attcgcttga
     3241 tatgaccaat atttcggctt acaatgcgct ttcaagtaca gatttgggaa aggtcggaat
     3301 cggtgctgag cgctccgagg tacttgagat ctacaggcaa atattgagcg caaacctcag
     3361 gcttcatgat gacctggagc cgaaaccgga ttctgttgcg gagggacgtg tggaagtatt
     3421 gtcactggag atttacagca gcgggttccc ggcgcagtgc cccgaagggg caacggtaac
     3481 gagaccgact gtccacagca gcatttgtgt gcctgtaagg ccgtcgttat acaggagcat
     3541 catacttaaa atgctgggca gggattatgt tgacataata gtgcataccg atactgaaat
     3601 tccggtaaac aactgatcaa aaatcaaggt acaggtgcgg ttatgagaag atatggctgg
     3661 atttgcggag cttttgttat aattatactg cttgtcgtga tcgagatgaa gataatctcg
     3721 aatgcttcgg ggtacgaagc aaaggaaagg gtcgtcgttg cagctaggga tatcggcgag
     3781 gataccgtta taaccggcga aatgcttgaa ataagggaaa taagcagcgg cgccgtacat
     3841 ggtgatgctg tcaggaagat cgaggatgct gtcgggatga cagcggcaga tcccatttat
     3901 gacggagagg tgctgctgaa aggcagattg acggaggaag cgtccgatac tgtggaggcg
     3961 ctgaacaaat caaacaggct gtactgcatc gaacccgaat gggaccaggc aaacgcctgg
     4021 cagctggaaa aggatcagca ggtggatgtg gtttttgtcc cgaaccatct gaaccaggac
     4081 aatgcctgcc cggaggcttc aggtgtggtc agtgtgacgc cttcgcagac cgggatgaaa
     4141 atattgaaaa atatcaggat agcaggcctt gtcgatgaag acggagttat catgaaaaac
     4201 tgcgagactg agaagatccc caaatacata atacttgagg tgacccagga tcaggctgtt
     4261 tttcttgctt atgcaaaaag taacggccgg atagagcttt tggccatacc gggccggaaa
     4321 ccgtgatgtt tgtccataag cgtgacattc ctcaactatg gggtcattcc ttaaccgtaa
     4381 aagaatacct ccttgcaaag gagtgccccc atactgtttt ccgaggagtg tccccataac
     4441 aattcaaatg cgtgacattc ctcagccatg gggacattcc ttaaccacag aggaatgctt
     4501 ccctgtaaag gagtgtcccc acaccacttt cagaaaggag cataaaaaat ggcggaactt
     4561 aagtacgaaa tcaggaaatt tcttggagtg atcggagaag gcaccaaggg gtggaaaaaa
     4621 gaagtcaacc ttatcagttg gaatgaccgt aagcccaaac tggatataag ggactgggac
     4681 gagaaccatg aaaaaatggg gaaggggatc acgctaagca ggagcgaggt ggaggaactg
     4741 aaaaggctgt tggcagcgtt cgatcccggg gaaatggagg cctgacggcc tccatttctt
     4801 ttggatccgg gaacataacc atcgaatcat acctttttgg cacagaaaaa atcctgttga
     4861 ctggaacaaa tggcaataca tcatcgtcgg taattggaaa tattttatgc aggagaatta
     4921 acatgaagac aaaacatatt gtactgctga tgatattatt aacttttata gcaatggccg
     4981 gatgcatgaa cggagagtcg gccggcaacg gtggcggacc ggtgaagccg gcatcatccg
     5041 acggcgggtc tgaaccggcg attgcctcgg acagcagcca ggcggcagtt acggaaccgg
     5101 ccgggcagac tggtgcagga cccgtcggca aagacggaga tagaggcgta gtcgaaatta
     5161 ctggcaatta cacccgtttt gacgggataa agggatttga cggtgattac cagtatctct
     5221 ccatggtagg cgaaaccgat ggccatattg tcatgcagta tatgcccgtt gagtacagta
     5281 aagaagtcaa gccttcactt atgctcgtcg atatcgatac tcttgaaata gccaggaatg
     5341 taaagttata caacgttctc cctgaaatcg gggaaaatcc tgtttcacac accagggtcg
     5401 ccggagacag gatcattgtt tgccttgaaa gcttggttgc ggtatttgac gaatcactgg
     5461 aactgataca ggagataccg ctgccggaag ctatagtcag aaaagccggc cggacggtta
     5521 catatgacaa gaacgggata atagaagtct tttacggcgg atatgacata tcggatgatc
     5581 tcgggaagat cgtctatacg gacgagaccg gcatttggct ttatgacctt gggtccggta
     5641 cggaagaatt gctagctgag aatatatata atgaagatta cctgaagagg agcttttacc
     5701 gtttcccgag attcatcgcg gacagcggga aaattatcgc agacaggctg ggctatgagt
     5761 attcgttggg atatgctgtc ttaagtatct ccggcaaatc gctccagaca ctggaccttt
     5821 tcagcgagcg ggggatgaac gatatctatt atgacacggg tctgctggaa ccggagacaa
     5881 atatgctcaa caaagaaacc ggcgactatg agcggaaatc gttctacctg gatttcgaaa
     5941 ccggcaatgt gaccgagata caaatagaga aaagcatata tgaccgttat cctttccggg
     6001 acaacttcta tgtaggtgaa aactatgcgg tttttgttac ttatatctgg gccgacgacg
     6061 gagattatta tgaaaccagg gatgatatgt tttacctgaa ccgcatagat ctcaatacgt
     6121 tggaaatgga aaccgatctg ctgtccgtca gggcgggcgg gccgcatatc ctgggagtgc
     6181 ttgcagacgg aagaatcctg ttccggtact attacgatga aaccgataac ggtgtctgta
     6241 tagccaggtg aggaaataat accttctatg gctgatgccc ggtccatgcc gtttcctgat
     6301 gcggtccctt cattgggcag ttcctgttcc ggccggcgga acggctgttt cgggaactgt
     6361 cccgatgttc cgtcgctttt caagcactgc cgccgatgtt ataatttaat tagaaagcag
     6421 aggtggtttg atggacaggc tcctgccgaa ggcatgggaa gggctgctga actcttcgaa
     6481 tttcctgccc gtgatagtgg ggacactgga gaggatccag gatgccacat ggtctatgga
     6541 acccaacaga catgataact atgaaatggt ctacatgaaa aagggctatg ctgtatttga
     6601 gatatcaggc cataaagtcg aactgggtcc caatgacatc attataatca aacccatgca
     6661 gtaccacaag ttcatcgtca agtcggagag cgggtgcgag ttcatcgtcc tctactttac
     6721 gttcgaaaac aggataaacg gcgagtatgc gcagataccg ctggaggatt tcctcaactt
     6781 cgtaagcaat aaagagtcaa gtccatatat aaaactgaag gtaagcccca aaaatgaaat
     6841 agtcgtcctg ctgaacagga taatgaagga aagggagagc tcggagccgg gcagtgaatt
     6901 cctcaactat ctgcttataa tggagttgtt cgtgctgctt tcgcgcgcac tgaagatgga
     6961 gtgggaaaac agcataaaac tcaaaagctc caagctaaag gagcttatcg gcatatcgat
     7021 aaactatatc cacaacaact tcgagcgtga tatctcgctg ggagacattg ctaagtacgt
     7081 tttcctcagc ccaagctatt ttaccagggc attcaaggag gaaacaggca tgagtcccat
     7141 cagctatctg ctgaaggtcc gcatcgagag ggccaaggag ctgcttgtgg acacaagtct
     7201 caaaatcagc gacatagcac tgaacgttgg tttttcaaat caacagcgtt tcaacgaaat
     7261 gttcaagaaa tatgccggcc tgactcccct gcagtacagg aagaaaaacg ccaggtagtt
     7321 gttcatttga caaaaaacat tcatattggc ccgttatttc agtaagtaac aggaagaatc
     7381 gcatcgtgtc aggcattatt atatatactg aatagccgga tcattattgg gaagtccgga
     7441 aggcacaggc tgccgggtgc cttgggaaca ggttgatgtt attgcagcgg aaacgcgcat
     7501 aatatgaaac gtgccggcat gtggcattgc tttgcttacg gacaggataa cgcagcgatt
     7561 ttctgactgt aaacggctca atttacgatg aaatatatga tgcgatattc tgcgcagtat
     7621 aaaatatact gctgtactgc agccgaatgg gagggatgat tcatggccga tacaggtaca
     7681 aaggcaagag gcatgacgat gacacagaag atactggcgg accatgccgg acttgacaga
     7741 gtggaagcgg ggcaactgat cagggcaagg ctcgatatgg tccttggaaa cgatatcacc
     7801 actcctgttg cagtgaagga gttcagaaag atcggcaggg accgcgtatt tgacaaaaat
     7861 aaaatagcga tcgttcctga ccacttcacg ccgaataaag atataaagtc ggccgaacag
     7921 gtcaggttca taagggaatt tgcccgagaa atgggcatag tgaacttttt cgaagtcggc
     7981 cgtatgggtg tcgaacacgc cctgctgcct gagatgggcc ttgtggtgcc cggggacgtt
     8041 gtaataggag ccgattcgca cacgtgcaca tatggcgctc tcggggcctt ttccaccggt
     8101 attggaagta cggacatggc ggccggtatg gcgaccggag aggcgtggtt caaaataccg
     8161 gaagccatcc ggttcgtgct gaccggcaga ccgggcaaat gggtgagcgg aaaggacatt
     8221 atactgcaca taataggcat gataggcgtg gacggagcgc tgtacaagtc gatggagttc
     8281 acgggagacg ggctcggaca cctttcgatg gacgacaggt tctcgatggc gaacatggcc
     8341 atagaagccg gagccaagaa cgggatcttc gacgtggatg aaaagactgt cgagtatgtg
     8401 aaagagcatt caacaaaatc atataagata tataaggctg atgaagatgc ggtatactgc
     8461 tgcgagtacg tcatcgacct gggtacggtg aagccgacgg tagcgttccc gcatctgccg
     8521 agcaatgcaa ggacgataga taaagtaggc agcatcaaga tagatcaggt ggtcataggt
     8581 tcgtgcacca atggaaggat agaggatatg aggacagcag cgcaggtact gaaaggacgt
     8641 accgtgcatc ctgacgtgcg ctgcatcata atccccgcca cccagaaggt atggaagcag
     8701 gcgatggacg aaggcctttt cgacatattc atagaagccg gcgccgctgt aagcacaccg
     8761 acatgcggac cttgcctggg cgggcatatg ggcatacttg caaaaggtga aagggctgtt
     8821 gccacgacca acaggaattt tgtcggaagg atgggccatc cggagagcga ggtttacctg
     8881 gcaagtcctg ctatagcagc agcatcggcg gttgcaggca ggatcgcttc gcccgatgaa
     8941 ctgtaaggac cggcagatgg acccgatgtg ccctggctga aagggcggac cggcagagag
     9001 ggcatattct gaaggactgt aaggccgcat cgaaggacag gaggtattta aatgaaggta
     9061 catggcaacg tgatcaaata cggggataat gtggacactg acgtcataat ccctgcaagg
     9121 tatctgaaca caagtgaacc caaggaactg gcggcgcatt gcatggaaga ccttgatccc
     9181 ggatttgccg gcaaggtcaa ggcaggcgat ataatagtcg cgggtaaaaa tttcgggtgc
     9241 ggttcctcaa gggaacatgc tccgatagcg atcaaagctt cgggaatatc ctgcgtcata
     9301 gcaaagacat ttgccaggat attctaccgc aatgccatca atatcgggct tcctattgtc
     9361 gagtgcccgg aagccgcaga ggatatcggc gacggtgatg ttgtcgaggt ggatttcgat
     9421 accggcacca tcacaaacaa aacaaaaaat aaatcatata aagcagtacc gttccctgga
     9481 ttcatgcagg agataatcaa agccgacgga ctcgtcggat atatcaggaa ccagggctga
     9541 tttgtccata gcatccatac atccggtgca cagccatgcg ggcattgtgc cgtgcggata
     9601 ttggcaaggc ggtgccgggg ggatcggaaa acaaccgggg atccatgggc atatagccgg
     9661 gtaacttacg gtaaaacctg aacaggagga aagacatttg aattacagaa tcgcagtcat
     9721 aaaaggcgac gggatagggc ctgagatagt cgaccaggcc atcgccgttc ttgatgcaat
     9781 cagcgaaaaa tgcggcatca gcttcacatt cagcgaagtg cttatgggag gctgtgccat
     9841 agatgaatat ggagtgccgt tgccggagtc tacgctggag acatgtaaag agagcgactc
     9901 tgtattgctt ggcgccgtcg gaggtccgaa gtgggaagga ctgccggggg acaggacgcc
     9961 cgaggcaggg ctgctcggca tacgggccgg gctcggcctg tatgcgaatc tcaggccggc
    10021 ggtcatatat gaagcgttga gggaagcgtc tccacttaag cctgaaatag tcggagggaa
    10081 aatagatatc cttgtcgtcc gtgaactgac aggaggcatc tatttcggcg aaaggggaag
    10141 aaacgggaag gggacgcccg atgaatgggc atatgatacc gaacggtatt ccgtgcctga
    10201 aattgagcgg atcgcaagga tagcgttcga agctgcctca aaacgccgcg ggatcgtcac
    10261 atccgtcgac aaggccaatg tgcttgaaag ttcgaggctg tggcgtgaag tcgtgacaag
    10321 ggtgtcaagg gattatcccg gcgttgcgct gaaccacatg tacgtggata atgccgccat
    10381 gcagctcgtc aggaatccgg gccagttcga cgtcatcgtg acgacgaaca tgttcggtga
    10441 tatattgtcc gatgaagctt caatgataac gggctctatc ggcatgctcc cctctgccag
    10501 cctgggaagc ggcaaaccgg ggctatatga gccgatacac ggctcagcgc ccgacatagc
    10561 gggacaggat aaggcgaatc cgatagccac catcctttct gcagcgatga tgctccgata
    10621 ctcgctgggc gagaatgccg cggcggatct gatcgagaat gctgtcgtaa aggtcctgga
    10681 gaagggttac aggactcctg acatagcgtc tttcggagga actgtcactg gtacgaagga
    10741 aatgggcagg ctcatagcgg aagaagtcag gcgggccgaa ccctgattca ccaggcttct
    10801 gcatgcccgg cgcttcgtcg ggacaccgat gatgcaaggg gtttgttcct gtgaccgaaa
    10861 aaaatcctct gatcaaggtg ttataattat actttaaaaa gtataaatag caatgtggct
    10921 ttccctgcat tgcttttttc atatgttatc aaaagctatt atatattatt atttattttc
    10981 acattttatt gttagaatat aaaaattctg ctttatattc tagcataacg taaaaatagt
    11041 aaacataaaa tttaaacata taacgaaaaa taatgtataa ttataacaaa acatagcagg
    11101 atttttgatc cggaaataaa accagcagat atcggagata agcgccccaa aatgccaaaa
    11161 agcctgaaaa tactgcacca gacagcatga tccgacgttt tgcatgctca ataacaggca
    11221 atacagtaaa aaattttagt gaaaaataaa tgctttttca aattgcatta tttttttctt
    11281 tcgtgtatta tgtttacaga ctatttttgg ggtcgtccgc tcgacgcggt cagttgtccc
    11341 tgttaggcta acagaaaggg tgtcttgatt ggggaagtat atagttaaaa gaatcctgct
    11401 tgcgatagtc acgctgtggc tggttgcaac aattacgttc atattgatga acatggttcc
    11461 cggcgggcct ttccttgctg agaaagcgcc gtccgaggcg acgctgaagg ccctgaacga
    11521 gaaatacggg cttgacaagc cgttgatagt tcaatacaag aactatttga ccagg
//
LOCUS       DAOSCC010000056        11414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3795_length_11414_cov_4.885289, whole genome shotgun sequence.
ACCESSION   DAOSCC010000056 DAOSCC010000000
VERSION     DAOSCC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_11414_cov_4.885289"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1584
                     /locus_tag="PLG72_04550"
     CDS             382..1584
                     /locus_tag="PLG72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ80102.1"
                     /translation="MYTLAFSMINNNNNLFAMRLGASDLQISLLTTFSQITGLTFLIP
                     GAILTDRLSDKRRMVTLALLMLSVSYFLIGFTPFFGSYRYYAFLVLMSISVCPLTLYN
                     TSWQAYFSDVIPIEHRNRILSSRTAGTFLAGMAVSLVCGNLLAAFEGYDAKIRVHQSF
                     FWTASVLLIIQIIVLNRIDHTGDGTRKAVKLSEIKDTIVGLSRNKLFLSFLGTSLFFH
                     MTWQSDWTLYFLGQVRYLGLNEAWLSYVSIGGTLAQFLSVGLWRRVNDKMGVRFGIIF
                     GNIGLAGCVASMLIATGVPASAGPVVFLVLNTLSNVTYPAVQMNLLQCLLQVAPEKNK
                     TLSISIYTMFITLSNAFMPMAGVAVYTALGGDLSAYRTTFWIIFCLRIISTGLWTLRW
                     YLLRNEPK"
     gene            complement(1681..>2742)
                     /locus_tag="PLG72_04555"
     CDS             complement(1681..>2742)
                     /locus_tag="PLG72_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80103.1"
                     /translation="FQNPFDAVIIFNDTEFGNRAELDNKLLELADASNVKITGRIDYT
                     CQVLLTVINGNTFAVPEPESTAYDSDVIRLILLDDYNAMENTNETLDDNEVFIFTSGP
                     DLKYDRIILGTKTFDVKKELDSAVFSRKADNNTFNPEYYMVVSSTDVMDKLLAEFGSA
                     ADRGRRYTIMFNLTGQENGKDNYVNSVSSWCESQPGFYNLTNGVAGKLRTLSMNGGLL
                     FIGIFFSIIFTMCLVLIMYYKQITEGYDDRDNFDIMQKVGMSDAEVKGTIRKQILMVF
                     YSPLVIALLHTMAGFNMIVRMFNALSLFGTGLIIRCGLIVAALFTVLYGFSYSYTSRT
                     YYGIVKQMKTDVAAERSIA"
     assembly_gap    2743..2842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2843..3570)
                     /locus_tag="PLG72_04560"
     CDS             complement(<2843..3570)
                     /locus_tag="PLG72_04560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ80104.1"
                     /translation="MNKLLLLPKLSVMGIRKNGTSYFPYIMAGSFSVFIFFVFASILA
                     NDIMYTLPRNSYALMLMRIGQILLGLILLPFIVYANSFFIKRRKKELGLYSILGLDKK
                     HIAAMMAWESLIIFSIVMIIGITFGLVFSKLMFLMLLNLTSLSVSASFGFSPVAFYQT
                     LLYFLAVYGINLVMNIIRVFRAKPNDLLTGAKKGDREPKHLWLTAVSGLVLLGMGYTI
                     SITSKLDSMIFMNFFLAVSLVIIGT"
     gene            complement(3563..4330)
                     /locus_tag="PLG72_04565"
     CDS             complement(3563..4330)
                     /locus_tag="PLG72_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80105.1"
                     /translation="MALLEVNNVKKVYSTRLGGNKVHALNGVTFSVEKGEYVAIMGES
                     GAGKTTLLNILATIDRPTDGQILLEGKDLATIPQRELAAFRRDNLGFVFQDFNLLDNF
                     TIQDNIFLPLVLAGTPLEEMKRRLEPLARKLGIEEILQKYPYEVSGGEKQRTATARAL
                     ITNPKIVLADEPTGALDSKASSRLLKLFAEINKEGQTILMVTHSINAASHAGRVLFIK
                     DGCVFHQIYRGNASNEEMYTMITDALTVIQTGGMKNE"
     gene            complement(4710..5780)
                     /locus_tag="PLG72_04570"
     CDS             complement(4710..5780)
                     /locus_tag="PLG72_04570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ80106.1"
                     /translation="MAKLKNTHTSAKNPAISKLPVHRLLYGYILERRKAAAVFLSGML
                     LFFAVCLLYHLENLSDLLYGFFLWAFTVLCFGIFDFVRYARRYEAFQAAIGNTENLFE
                     LLPPPSGVMDQQYHQIIEKLTSDLTRLRSEMSLKDADMSDYYSMWAHQIKIPISAMRL
                     LLQNSSEDKSVPRLLSEELFKIEQYVDMVLYYQRLESISSDFLFRKYDLHDIVKQALK
                     KYATLFINSKLSLDLQEFSCLVVTDEKWLQFALGQIISNALKYTPRGGISIYLETTPE
                     NAYLVIEDTGMGIRPEDLPRIFERGYTGYNGRLDKKSTGIGLYLCKKILDKLSHTVEV
                     TSEVGKGTRVIVGFSLESAEGL"
     gene            complement(5828..6505)
                     /locus_tag="PLG72_04575"
     CDS             complement(5828..6505)
                     /locus_tag="PLG72_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ80107.1"
                     /translation="MYRILLVEDDDVIANVIREYLNRWGYDVYCVDDFNDVTGCFSRV
                     EPQLVLMDIYLPFCNGYHWCKEIRKLSRTPIMFISSASDNMNIVMAVNMGGDDYIQKP
                     FDLEVLRAKIEALLRRTYAYNEQSAWLEHKGVLFNTLDGTITYQGKKVELTKNEFKIM
                     QLLFENIGKTISREAIMKRLWDDDCFIDDNTLTVNMNRLRKKLEEASIRDFIRTKKGL
                     GYLIESE"
     gene            6879..8903
                     /locus_tag="PLG72_04580"
     CDS             6879..8903
                     /locus_tag="PLG72_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOJ80108.1"
                     /translation="MASGRLRLPRLISDGMVIQRDKAVKIWGWAGKGDIVTVRLTLQA
                     HEHPVSTGMDAGSDGHVYGYAAEQVHECTAGTDGRWEIMLQPLEAGGPYTLAVSAARG
                     SNENCAGGCGEDNAAAAEQITVGDIFAGDVWVCSGQSNMQTPISRLADLFGKEIAEAC
                     SSEIRYFQVPERYDFNKVHEDLEGGSWASCEAPEKVMGFSAVPLFFASELHKKYNIPV
                     GVINASVGGSPIEAWISEDSLADWPDSLEELARCKDDAYVGEVKKRDMEAMSRWFGQL
                     DRLDEGLGSPGEPLYSKDIDTSGWADISIPTSWSDAGLKNFYGSIWLKKTFSLPARLA
                     GKEAILRLGTIIDSDVAFVNGVQVGNVTYRYPPRKYVIPAGLTKEGENTITIRVISNS
                     GDGGFVEDKPYSIEIGDMVIDLTGRWKYKIGAAMEDPLPEMTFFNWKPAGLYNGMLAP
                     LHSYAVKGIAWYQGEANTDRPGRYFGLFRKMVGSWREAWGYDRLPFLYVQLQNWQRPV
                     SEPGDSQWARLREQQLKALSLPDTGMASAVDAGEWNDIHPLDKLTVARRLALAAYKVA
                     YGENIIASGPVYKDMVKEKNRIIIRFMEPTGGLVSVDGKPLRHFAVAGPDRRFVWADA
                     KIDGDTVIVWNDEIEDPVAVRYAWADNPEGANLYNKAGLPASPFRTDDWD"
     gene            complement(8913..10058)
                     /locus_tag="PLG72_04585"
     CDS             complement(8913..10058)
                     /locus_tag="PLG72_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOJ80109.1"
                     /translation="MNRHKRLLALITIVLLFSILTSSTCVFAASKPVLRKGMRGEAVR
                     TLQINLKKLGFFNAEPTGYFGDITYAAVKKFQKKYNIPTTGVVASLTHAKMDELLKDR
                     KKSLPDGLTQGATGSEVKKLQEKLNLLGYMKVTPTGNFGPITESALVQLQKYHGMEPH
                     GVADKETLSLIDRLVGQIEHSSRGSGTGTDIPVKIKPLYKVKTEWIPDLPKAPYLKGI
                     GKYEGVVIHYTDSPGDNARMEANFVRTNWQNAFVHEFIDADEIIQVADPDYKAWGAGQ
                     AANDRYIHLELCHEYTRSDFEASYKKLVRRAAEYLYMNQLGVTPAKPDKSGTLWGHYH
                     VTLYWGGTDHTDPIKYLAGWGITWNELVADVEEEYRAIEAQYGYSDK"
     gene            10349..>11414
                     /locus_tag="PLG72_04590"
     CDS             10349..>11414
                     /locus_tag="PLG72_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOJ80110.1"
                     /translation="MKKFDKKILMILMVIITTAVVVAMLVRSTKAFEVFLDDRSVGVV
                     DNQSTFTELVENIKKNAESRYGTEMLVASRIEYKEVYLPDHDRVIDASSEESVSQVEK
                     SVLLDAKVFAINVNGEDIAYFRDRASAEQVLEMVRAPYVQNAGEGVSTCFVENVSIKE
                     RVFPIQQLPELQDPEEVYSSIMQENETIKKYVVQKGDTVSEIAEKLGVSIKDIVKANP
                     DLDIDRISIGQELNLAVPRYRINVMQSRTTVYEDAAPCPVEYEETDELYKGEYKVKVE
                     GVEGTKLVKACYISINGFPEETRILDETIIKEPTTEIVLKGTKQRPRTMAYGVFYWPS
                     RGRITSRYGPRWGEKHSGIDI"
BASE COUNT         2747 a         2690 c         2939 g         2938 t
ORIGIN      
        1 tcaaggtgtc tcctttaatt ttacattata tgttagcgct tttgctcatt tatagcatgt
       61 attattcttt gataaggact ggttaattat gctgtatctg tggtaatttt agtttggagc
      121 aggtttcagc cggcattatt gctcgatttc cggacgccgg acagtgaaga cggatcgtga
      181 aacggatgat tgccgtccgg aggactgcgt tgtataattg acatattgtt tgtgctgttg
      241 aatttgtgct tactgaaagg ttgaagtggg tttatggtcc cgcaattgat gaaaccgctg
      301 cgtgcttttc gccgcaaata cgcctatgtc actgctcagc cgtccggccg ggctgctgtt
      361 tttttcacct ttgaaggcat attgtacaca ttggcattca gcatgatcaa caacaataac
      421 aacctgttcg cgatgaggct tggcgcaagc gatctgcaga tcagcctgct gaccacattt
      481 tcgcagataa ccgggctgac gttcctgata ccaggcgcca tcctgaccga caggctgtcg
      541 gacaagcgca ggatggtgac cctggcgctg ctgatgctgt ctgtgtcata cttcctgatt
      601 ggatttactc ctttttttgg atcttacagg tattacgcgt tcctggtcct gatgagcata
      661 tcggtgtgcc ccctgacact gtataacacg tcgtggcagg cctacttttc tgatgtgata
      721 ccgatcgaac acaggaaccg gatcctgtct tcgaggacgg caggcacatt tctcgccggc
      781 atggctgttt cgcttgtctg cgggaacctg ctcgccgctt tcgagggata cgatgccaag
      841 ataagggtac accagtcatt tttctggacg gccagtgtgc tgctcataat acagataatt
      901 gtactcaacc ggatagacca tacgggcgac ggtacccgaa aagctgttaa attgagcgaa
      961 atcaaagata cgatcgtcgg attgtccagg aacaaactgt ttcttagttt tctcgggact
     1021 tctttgtttt tccatatgac atggcaatcc gactggacat tgtacttcct cggccaggtc
     1081 cggtatcttg ggctgaatga agcatggctg agctatgtga gtatcggggg tacgctcgcc
     1141 cagttcctgt ccgtcggcct gtggaggaga gtcaacgata aaatgggcgt caggttcggc
     1201 attatcttcg ggaacatcgg cctggccggc tgcgtggcca gcatgctgat cgccactggc
     1261 gtgccggctt ccgcgggacc tgtcgtgttc ctggtcctaa acactttgtc aaacgtgaca
     1321 tatcccgcag tccagatgaa tctgctgcaa tgccttctgc aggttgcccc tgaaaagaac
     1381 aagacactca gcatatcgat atatacgatg ttcataactt tatcgaacgc gttcatgcct
     1441 atggcgggag ttgctgtcta cacggctctt gggggagatc tcagtgctta tcgtacgacg
     1501 ttctggatca tattctgcct taggataata tcgacaggat tatggacgct gcgctggtat
     1561 ttgctccgca atgagccaaa atagcaggga ggtgaagagg agatcactcc aggggaaggc
     1621 gaagcaccag ggaggcaaat cagtgattgc cccctgaccg gttgcctttg caggtgcatt
     1681 tcatgcgatg ctgcgctcgg ctgcaacatc ggttttcatc tgctttacga tcccgtaata
     1741 agtcctcgac gtgtaactat agctgaagcc gtacaggacc gtgaacaagg ctgccacgat
     1801 aaggccgcat ctgatgatga ggcctgtgcc gaagagcgac aacgcattga acatccttac
     1861 tatcatgttg aaacccgcca ttgtgtgcag gagtgcgatg acaagcggtg agtagaagac
     1921 catgaggatc tgcttcctga tagtaccctt tacttctgcg tcgctcatgc ctactttctg
     1981 cattatatcg aaattatcgc ggtcgtcata cccttctgtt atctgcttgt agtacatgat
     2041 cagtaccagg cacattgtga atatgatgct gaagaagatg cctatgaaca gcagcccgcc
     2101 gttcatggac agggtcctga gcttgccggc gacaccattt gtgaggttgt agaagccggg
     2161 ctgtgattca caccaggaac tcacgctatt tacataattg tccttgccgt tttcctgacc
     2221 tgtcaggttg aacatgatag tatatcttcg gcccctgtcc gctgcgctgc cgaattctgc
     2281 caacagcttg tccatcacat cagtgctgct tacgaccatg taatactccg ggttgaatgt
     2341 gttgttatcg gcttttctcg aaaacactgc cgagtccagt tctttcttta catcaaatgt
     2401 ttttgtgccc agtatgatcc gatcatattt taagtcagga cctgacgtga atatgaagac
     2461 ctcgttgtcg tccagggttt cattcgtgtt ttccattgca ttataatcat caaggagtat
     2521 cagcctgatg acatcactgt cataagcagt actttccggt tccggtactg cgaaggtatt
     2581 tccatttatg accgtcagga gtacctggca ggtataatca atcctgcctg ttatttttac
     2641 atttgaggca tcagccaatt ccagcagctt attgtccaat tcagccctgt tgccgaactc
     2701 tgtgtcattg aaaattatga cggcatcaaa tgggttttgg aannnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nngtccctat tatgacaagc gaaacggcca ggaaaaagtt
     2881 catgaagatc atggaatcca gttttgatgt aatggatatc gtatatccca tgcccagaag
     2941 tacaaggcct gatacagctg tgagccagag atgctttggt tccctgtctc cctttttcgc
     3001 acctgtcagc aaatcattgg gttttgccct gaaaacccgg ataatattca tgaccaggtt
     3061 gatcccgtat acggcaagaa aatacagcag agtctggtaa aaagcaacgg ggctgaatcc
     3121 gaaggacgcg ctgacagaca gtgacgttaa gttcagcagc ataaggaaca tcagcttcga
     3181 gaatacaagc ccgaatgtga tcccgatgat catgactatg ctgaaaatga taagggattc
     3241 ccatgccatc attgccgcga tgtgcttctt gtcgagcccg aggatactgt aaaggccaag
     3301 ttcctttttc ctgcgcttga taaagaaact attggcgtag acgatgaatg gcagcaatat
     3361 cagccccagc aggatctggc cgatccgcat gagcatgagg gcatagctgt tccggggcag
     3421 cgtgtacatg atgtcgttgg ccaggatcga tgcaaatacg aagaaaatga atactgaaaa
     3481 actgccggcc ataatatacg ggaaatagga tgtgccgttt ttgcggatcc ccatcacgga
     3541 cagttttggc aaaagcaaca gcttattcat ttttcatgcc tcctgtctgt atgaccgtca
     3601 gtgcgtcggt tatcatcgtg tacatttctt cattggaagc gttgccgcgg taaatctggt
     3661 ggaaaacgca gccgtccttt atgaacagca cccttcctgc atggcttgcg gcattgatgc
     3721 tgtgggtgac catcaatatc gtctgcccct ctttgttgat ttccgcaaac agcttaagca
     3781 gccttgacga tgcttttgag tccagtgcgc cggtaggctc gtccgcaagc acgattttag
     3841 gatttgtgat cagcgcccgc gctgtggccg tcctctgttt ttcaccgcct gatacctcgt
     3901 acgggtattt ctgcaatatt tcttcgatcc cgagtttcct tgccagcggt tcaaggcgcc
     3961 ttttcatttc ttcaagcggt gtcccggcca ggaccagcgg aaggaaaata ttgtcctgga
     4021 ttgtaaagtt gtcgagcagg ttgaaatcct ggaacacaaa gcccaggttg tcacggcgga
     4081 atgctgccag ttcgcgttgg gggatcgttg ccaggtcctt gccttccagc agtatctgtc
     4141 cgtcggtcgg acggtcaatt gtggccagga tgttgagaag ggtcgtcttg cctgcgccgg
     4201 attcgcccat tatggcaaca tattcgccct tttcaacgga aaaggtaact ccgttcagcg
     4261 catgcacctt gtttccgccg agcctggtgc tgtaaacttt tttaacgttg tttacttcaa
     4321 gcagtgccat taccatcttt cctttctttt cttctctctt tccatttctg tttctgccca
     4381 atacctgtgc ggctatttaa atatgcctgg catatgccgt gaaataccgt tgttttttct
     4441 gtatactttc cgggctgctt ctattttgat tttatatccg tctgtggtcc ggtgccataa
     4501 tacttcctta catttcattt tgcaaactta catttttgta atgaaacagg ggagattgcg
     4561 aggaaacagg agtgatttga tgcatggacg caaacatatg atacatttgc gacgattccg
     4621 ctttgcgaaa ttacggccct gacttcggtg atccgtgaaa aaactatttg atatccgggc
     4681 aataacggct ggatgcgcag gatttcaact cacagacctt ccgccgattc cagcgaaaat
     4741 ccgactatga cccttgtgcc tttcccgact tcagaggtta cttcgacggt gtgggacagc
     4801 ttgtccagta tctttttgca caggtacagg ccgatccctg tggacttctt gtcaagcctt
     4861 ccgttgtagc ctgtataacc acgctcgaaa atgcggggca ggtcttcggg acggatcccc
     4921 atgcccgtgt cctcgatcac tagataagca ttctccggag tggtttcaag atatatcgat
     4981 atgccgccgc gaggggtata tttcagtgcg tttgaaatga tctgcccgag cgcgaactgc
     5041 agccattttt cgtcagtgac cacaaggcag ctgaattcct gcagatcaag ggacagttta
     5101 ctgttgatga agagggtcgc gtattttttc agcgcctgtt tcacgatatc atgcaggtca
     5161 tatttcctga acaggaagtc ggaggagatg ctttcaagcc tctggtaata caataccatg
     5221 tcgacgtact gctctatctt gaataattct tcggacagca gtctggggac tgacttgtct
     5281 tcgctgctgt tctgcagaag cagcctcatg gccgaaattg ggattttgat ctgatgggcc
     5341 cacatcgaat aatagtcgct catgtccgca tccttcaggc tcatttcaga cctgagccgt
     5401 gtgaggtcgc tcgtcagttt ctcaatgatc tggtggtact gctggtccat tacgcctgaa
     5461 ggagggggaa gcagttcaaa aagattctcg gtattcccga tagcagcctg gaatgcctcg
     5521 tatcttctgg catatctcac aaaatcgaaa attccgaaac acaataccgt aaatgcccac
     5581 aggaagaatc catacagcag gtctgacagg ttttccaggt gatacagcag gcataccgcg
     5641 aaaaacagga gcatgccgga caggaaaacc gctgctgcct tgcgcctttc caggatatat
     5701 ccgtaaagga gccgatgtac aggaagtttg cttattgcag gattttttgc tgaggtatgt
     5761 gtatttttca gtttggccat gattttcacc ttcgttcgga taaccaaagc tctctgccgg
     5821 acctttgtta ctcgctttcg atcaggtagc ccaaaccttt ttttgtgcgg ataaaatccc
     5881 tgatcgaggc ttcttcaagt tttttacgca gcctgttcat gtttaccgtg agcgtattgt
     5941 catcgataaa gcagtcatcg tcccataacc gcttcattat ggcttctctc gaaatcgttt
     6001 ttccgatatt ttcaaaaagc aactgcatta ttttgaattc atttttcgta agctcgacct
     6061 ttttcccctg gtatgtaatc gtcccgtcga gggtgttgaa cagcactccc ttgtgttcga
     6121 gccatgcgga ctgttcatta tacgcgtaag tccgccggag cagcgcttct atcttggccc
     6181 tcaggacttc caggtcgaat ggcttctgga tataatcatc tccgcccatg tttaccgcca
     6241 ttacgatatt catgttgtcc gaggcagaag aaatgaacat gatcggagtg cgggacagtt
     6301 tgcggatttc cttgcaccag tggtaaccgt tgcagaacgg aagataaata tccatcagta
     6361 caagctgagg ctcgacccgg ctgaaacagc cggtaacatc attgaaatca tcgacgcagt
     6421 atacgtcata accccagcgg ttgagatatt cccggataac atttgctatt acgtcatcgt
     6481 cttcgaccag cagtatcctg tacataagcc cgcagagcct cccttgccat aaggatgatc
     6541 ataccgctgc caaagccgca gatcaagtgc cgtcccgtcc gtgtttgctg cagcccggaa
     6601 tctgcggcct gttatttgat attcggcatt tctgaccagt tatctgctgt ataaaatgtt
     6661 atcatttata ttgaaaaaat aaaagccgtg ctgaaggcag caggacaact caccatatcg
     6721 ggcctgaccg gttttcgcaa atctgcggcc ggatattgac attatccgga aactggcgga
     6781 ttataaaaat agtatggaaa cactgatgat aaaccgataa gactgatata gcatatggat
     6841 gtgttcgaat attcatcatg aaaggtcgtg gatcacagat ggcttccggc agattacggc
     6901 ttccgcgcct gataagcgac ggtatggtta tccagaggga caaggcagtc aaaatatggg
     6961 ggtgggccgg taaaggcgac atcgttaccg tgaggctgac cctccaggca catgagcatc
     7021 cggtgagtac cggcatggat gccggatctg atggccatgt gtacggatat gcggcggaac
     7081 aggtacatga atgcacagca ggtactgacg gcaggtggga aatcatgctg cagccgttgg
     7141 aggccggcgg tccgtacacc cttgcggtga gcgctgccag gggcagcaat gagaactgcg
     7201 ccggaggatg cggcgaagat aatgccgctg cggcagaaca gataaccgtg ggagatatat
     7261 ttgccggcga cgtatgggta tgttccggac agtcgaacat gcagacgcca atatccaggc
     7321 tggcggacct gttcggaaag gagatcgccg aggcatgcag cagcgagatc aggtatttcc
     7381 aggtaccgga acggtatgat ttcaataagg tgcacgaaga cctggaaggc ggcagctggg
     7441 ctagctgcga agcacctgaa aaagtaatgg gattttccgc agtgcccctg ttttttgctt
     7501 ctgaactgca taaaaaatat aacatcccgg ttggggtcat caatgcaagc gtcggcggtt
     7561 cccccatcga agcgtggatc agcgaggatt cgctcgcaga ctggccggac agcctggagg
     7621 aactggccag gtgcaaggat gacgcatatg tcggcgaagt aaaaaagcgc gatatggaag
     7681 ccatgtcccg gtggttcggg cagctggacc ggctcgacga aggactcggc agtccggggg
     7741 agcctttgta cagcaaagat atcgacacgt cgggctgggc ggatataagc atcccgacaa
     7801 gctggtccga tgcgggattg aaaaatttct atggatcgat atggctgaaa aagacgttca
     7861 gccttccagc caggcttgca ggcaaagagg cgatactgag gctcggcacc atcatcgaca
     7921 gcgatgtggc atttgtgaac ggcgtccagg taggaaacgt cacataccgt tacccgccga
     7981 gaaagtatgt tataccggcc ggattgacaa aagaaggcga aaacacaatt accataaggg
     8041 tcatcagcaa ttccggagac ggcgggtttg tcgaggataa accctacagc atcgaaatcg
     8101 gcgatatggt catcgatctg accggcagat ggaaatacaa aatcggagcc gcaatggaag
     8161 acccgctgcc cgaaatgaca ttcttcaact ggaagcctgc cgggctgtat aacggcatgc
     8221 ttgcgcccct gcacagctat gctgtgaaag gcatagcatg gtaccagggc gaagcgaata
     8281 cggacaggcc cggcagatat ttcggcctgt tcaggaagat ggtcggcagt tggagagaag
     8341 catggggata tgaccggctc ccgttccttt atgtacagct gcagaactgg caaaggcctg
     8401 tgagcgagcc tggcgacagc caatgggcac ggctcaggga gcagcagctc aaagcgctgt
     8461 ctttgcccga taccggaatg gcttcggcgg tagatgcagg cgaatggaac gatatacacc
     8521 cgctggataa gcttactgtg gccagaaggc ttgccctggc cgcgtataag gtagcatacg
     8581 gggaaaatat catagcttcg ggccctgtct acaaggatat ggtgaaggaa aagaaccgca
     8641 tcattatacg attcatggag cccactggcg gtttggtgtc cgtcgacggc aagcctctga
     8701 ggcatttcgc cgtggcaggg cctgatcgga gatttgtatg ggctgatgcg aagatcgatg
     8761 gtgacactgt cattgtctgg aatgacgaaa tagaggaccc ggtggcggta cgctatgcgt
     8821 gggccgacaa tccggaggga gcgaaccttt acaacaaggc gggcctgcct gcttcaccgt
     8881 tcaggaccga cgactgggac tgaggcgcag gatcatttgt cagaatagcc atactgggct
     8941 tctatagccc tgtactcttc ttccacgtct gcgaccagtt cattccatgt tatgccccaa
     9001 cctgccaggt acttgatggg atcggtgtgg tcagtgccgc cccagtataa agtaacatgg
     9061 taatggcccc agagcgtgcc cgacttgtcc ggttttgccg gagttactcc aagctggttc
     9121 atatagagat attcggctgc cctcctgacg agttttttgt atgaagcctc gaaatcactc
     9181 cgtgtatact catggcacaa ttccaggtgt atgtacctgt cgttggccgc ctgcccggct
     9241 ccccacgcct tgtaatcagg atcggcgacc tgtatgatct catccgcgtc tatgaattcg
     9301 tgcacaaaag cattctgcca gttggttctg acgaagtttg cctccatcct tgcattgtca
     9361 ccggggctgt ccgtgtaatg gattacgacg ccttcatatt tgcctatgcc cttaaggtac
     9421 ggtgctttcg ggagatcagg gatccactct gttttgacct tgtacagggg ctttattttc
     9481 acggggatat cggttcctgt cccgctgccc ctggaagagt gttcgatttg cccgacgagc
     9541 ctgtctatca gggataatgt ttctttatca gctactccat gaggttccat gccgtggtac
     9601 ttctgcagct gtaccagcgc tgactcggta ataggcccaa aattgcccgt gggcgtaact
     9661 ttcatgtatc ccagcaagtt cagcttttcc tggagctttt tcacttcgct gcctgttgcg
     9721 ccctgggtca gaccatcagg caatgacttt ttcctgtctt tgagcagttc atccatcttt
     9781 gcgtgtgtca gggaagcgac gacgccggta gtgggtatat tatatttctt ctggaacttc
     9841 tttaccgctg catatgttat atcgccgaaa tacccggtgg gttcggcgtt gaagaaaccc
     9901 agctttttca gattgatctg gagtgtcctg acggcttcgc ccctcatgcc ttttctcaaa
     9961 acgggtttag acgccgcaaa aacgcacgtt gaggaggtca ggatcgaaaa cagcagtact
    10021 attgttatta aggcaagtaa tcttttgtga cgattcattg gaagcaaccc ccaaagacaa
    10081 ctgaaaccaa tgcaacttat cgacaatttc tcttactata tttcaatatt accatatttt
    10141 tgacacattt aaaacaagga atttcagagg agttgcggga tggatggcgg gatgcggtca
    10201 cagaatatcg aaaagcgttt aaaaaattaa cgaattgttc ataattttta atgtccggtt
    10261 atgatataaa tttaagaggt ccttttttgg atcgactgta tgttaacgaa tgttgaaaac
    10321 ggacattgta aaaatggacg gggctgatat gaaaaagttt gataaaaaga tactgatgat
    10381 cctgatggta atcattacaa cggcggtcgt cgttgcgatg cttgtccggt cgacgaaggc
    10441 tttcgaggtc ttcctcgacg acaggtccgt cggtgtcgtg gacaatcagt ccactttcac
    10501 ggaacttgtg gagaacataa agaaaaatgc cgagagccgc tatggcacgg aaatgcttgt
    10561 cgcaagcagg atcgagtata aggaagtgta cctgcctgac cacgacaggg tcatagacgc
    10621 atcttcggaa gagtcggtat cgcaggtgga gaaaagcgta ctgctggatg ccaaagtatt
    10681 tgcaatcaat gtcaacggcg aggatatcgc atatttccgt gacagggctt cagccgaaca
    10741 ggtgctggaa atggtcaggg ccccctatgt gcaaaatgcg ggagagggcg tctctacctg
    10801 ctttgtagaa aacgtcagta taaaggagag ggtcttcccg atccagcaac tgccggaact
    10861 gcaggatcct gaggaagttt acagcagtat tatgcaggag aatgagacca taaagaaata
    10921 cgtggtgcag aaaggcgata cggtcagtga gatcgcagaa aagctcggcg tcagcatcaa
    10981 ggatattgta aaggcaaatc ccgacctcga catagacagg atctccatcg ggcaggaact
    11041 gaaccttgcc gtgccgagat acagaatcaa tgtgatgcag tccaggacta ccgtttacga
    11101 ggatgccgct ccctgtcccg ttgaatacga ggagactgat gagctctaca agggggagta
    11161 caaggtcaag gtcgagggag tggaaggcac gaaactggta aaggcgtgct acataagcat
    11221 aaatggtttc cctgaagaaa caaggatcct ggatgagacc atcattaaag agccgacaac
    11281 ggaaatcgta ctgaaaggaa ccaagcaaag gccgcgtacc atggcttacg gcgtcttcta
    11341 ctggcccagc aggggcagga taacctcgcg gtacgggccg agatggggag agaaacattc
    11401 gggaatagac atcg
//
LOCUS       DAOSCC010000057        11395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3804_length_11395_cov_4.203086, whole genome shotgun sequence.
ACCESSION   DAOSCC010000057 DAOSCC010000000
VERSION     DAOSCC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3804_length_11395_cov_4.203086"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLG72_04595"
     CDS             <1..299
                     /locus_tag="PLG72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ80111.1"
                     /translation="EKITGRKYDRIHIVGGGSGNALLNAFTANCCGKPVYAGPQEATT
                     LGNGIVQMISLGEVSGFTQARRIVKNSFPPAEFMPRDTRQWDEAYQRFIEMIRK"
     gene            complement(451..1095)
                     /locus_tag="PLG72_04600"
     CDS             complement(451..1095)
                     /locus_tag="PLG72_04600"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HOJ80112.1"
                     /translation="MFKKWEILKKITDSGLVVVVRADNADMAKRITDACLEGGAAAIE
                     ITYTVPGATKVIEELAKEYAGKIIIGAGTVLDAETARIAILSGAQYVVSPYLSRETLK
                     LCNRYQVPCMPGVMTIEGVVEAMELGADILKVFPGEAFGPGIIKSIKGPLPQANLMPT
                     GGVSIDNVGDWIKAGAVAVGAGGSLTAGAKTGDYASIRKLAAEFAAKIKEARQG"
     gene            complement(1140..1715)
                     /locus_tag="PLG72_04605"
     CDS             complement(1140..1715)
                     /locus_tag="PLG72_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOJ80113.1"
                     /translation="MNDIKVDPFAMYFDLITGMSDERKPTRRFLSQMRGMYADDAAYE
                     AAVGANDEIVYDFYELGLPETSGNLLFGTSIVYPGKVGNEYFMTKGHFHTILDTAEVY
                     YCLSGKGYMLMENPEGDWDAQLLTPGRAVYVPGRYAHRSINIGDEKLVTFFVFRADAG
                     HDYGTIETKGFRKLIAEKDGKPVIIDNPKWK"
     gene            complement(1795..2745)
                     /locus_tag="PLG72_04610"
     CDS             complement(1795..2745)
                     /locus_tag="PLG72_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ80114.1"
                     /translation="MNLPGKATQPAFYFIGVTTTKSSIMKVFPLWAKALGLKDAVIKG
                     IDIEIHANPEVYREVVDFIKNDELSLGALVTTHKIDLYNAAKDLFDYLDPYAVMFSEL
                     SCISKKDGRLEGYAKDPITAGLSLEAFVPKNFFKEHGGDVLLLGAGGSAVAMAAYLLN
                     MDKGDNIPDRLIVGNRSKPRLDHIESIVRQIRPDANAEFHLTPGPGQNDELLHRLKPY
                     SLIVNATGLGKDRPGSPLTDEARFPENSLVWEINYRGDLLFMHQALAQKEAKNLHVED
                     GWTYFIHGWTQVIAEVFHIEITPELFGECKKIADEVRNNG"
     gene            complement(2923..3933)
                     /locus_tag="PLG72_04615"
     CDS             complement(2923..3933)
                     /locus_tag="PLG72_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HOJ80115.1"
                     /translation="MESVLNRKENRMQTHPDEYIIQTVTGRISPDRLGYCQSHEHLFI
                     ADGYSAKLVPSLRLDDYDATCSELELYKSIGGKSIVDAQPVGCGRMADLQLKASLRTG
                     VNIIASTGFHKLVFYPDSHWIRKMDEDCLAGLFIDELENGMFIDCDISKPVRRIESRA
                     GVIKTASDMEGPAGDYRRLFSAAAEASVRTGAPILSHTELGMGALDQIRLFTDHGVPV
                     SSIIICHLDRVLTDPGHLAEVASTGVYLELDTIGRFKYHSDDDEARFIISLLENGFED
                     RILLGLDTTRERMKSYGGSIGLDYLHEAFFPMLRSYGVSDSHIYKFTVSNPAKAFSRK
                     KT"
     gene            complement(3917..4885)
                     /locus_tag="PLG72_04620"
     CDS             complement(3917..4885)
                     /locus_tag="PLG72_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ80116.1"
                     /translation="MEKKILITPKSFRNYKHKAYPLLEEKGYGIIENTFGRTMAENEI
                     TELAGEGVVGIIVGVDPLPGTVLERLRDLKAISKYGVGMDNIDLDRAAELGINVKNAV
                     GTNSVSVAELAIALMFTMARKIPFHAAEVKNGGWERSIGFELTGKRLGLIGCGQIGRE
                     VAKRARGLEMEVSVYDPYFMDDGFLSEYKVNRAGSIDEILSASDIVSLHLPATPETKK
                     MINSGTLGLMKPDAILINTSRGELVDEDALYEALVSGRIAGAAQDVFSSEPPANDEKL
                     LKLDNFLLTPHLGAFTGEATEKMALVSTKNLLEMLGHKEQSHGKRS"
     gene            complement(5012..5770)
                     /locus_tag="PLG72_04625"
     CDS             complement(5012..5770)
                     /locus_tag="PLG72_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ80117.1"
                     /translation="MFLGGNKTLNKNIPIPLYYQLKTILLEYIREEHKDLEKPIPTEV
                     EISEYFGISRPTVRQAINELVVEGYLYRVKAKGTFVSKPKITQDFLLTLDSFNNEMRK
                     KGLVPSTKILEKEVIKSDEKVAKTLGLGPGTDVIKLTRLRFADDDPIVFVITYIPYSV
                     CPPIMGHDLENESLYEIMEKESGRLISNAVRTLECITAGEFESKLLKVEKGSPIQYFE
                     SIVYLEDGTPVEYSLAKYRGDRSKFTFELKRQLR"
     gene            5938..7026
                     /locus_tag="PLG72_04630"
     CDS             5938..7026
                     /locus_tag="PLG72_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOJ80118.1"
                     /translation="MQKGKVRNMFSGGNTSKGFFSRFDHIMSHEDARRIFVIKGGPGT
                     GKGTFMKNISEIMTNRGYDTEHIHCSSDSKSLDALVIPELKVALLDGTAPHVIDPKAP
                     GAVDEIINLGEYWCGSALAEKREEIMKISNEISVFFQRAYRYLKAARHIYEDSSILYG
                     SAMDKAGLNSIAGEFTGMLFDVFPPACKPGRQRCLFASAITPDGLVSYVDDLMTLDSI
                     YVFEGFPGSGTDIVLEKIKEAAVERGFNVEAFYCGFDPDKLEHLIIPELNTGFSTAGK
                     YHSTDACVNKRVDFRELLNDSAISKLGPELEYNDHEFGSLLNKAVEMISNAKKLHDEL
                     EAFYIQNMDFEGIRKKQDETVQKILSYS"
     gene            complement(7124..9400)
                     /gene="csaB"
                     /locus_tag="PLG72_04635"
     CDS             complement(7124..9400)
                     /gene="csaB"
                     /locus_tag="PLG72_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOJ80119.1"
                     /translation="MRVVHIIGGGDVGGAKTHMLYLLKELSRHMEVKLISLRPGVFAD
                     DARALGIDVTVVKSANMYNDIRKVRKIIREGNYDIVHSHGAKANIFSLAATRKTDIPT
                     VTTIHSDYRLDYMHSLPKRLTIGLINSIALRFIENHIGVSDNFRKMLIERKFNPLDIY
                     VLYNGMDFSQPSVQYSRKKFLDKYRLDVKDDEVLVGIAARLYPVKNIETIVRAAHIVK
                     ARNRKIKFIIGGDGEDRKKLESLTAELGLQDTVFFVGWQDDPNELMSSIDISVLTSIS
                     ESFAYSVLEGARFKKATISTDVGGIPDLIEHGTNGYLFKPGDVERFAELILELAADEK
                     KRREMGERLYEKAYAKYSVETMGRTQIDIYRSILEKKAESGSRRKHYDAIISGYYGFR
                     NIGDDALLMSILKDLKSFRPNMRLMVLSKTPVATALEFNVASIDRAGMFRIYRAMRKS
                     KAFIYGGGNLLQDNTSTRSLLFYLSMVWLAKRLGLKVMFYANGIGPLKKSMNRLLTKK
                     IINKVDVITLREALSFDELKTLEISRPRILMTADAAVTVMDGAENIKTGIMERLGLAG
                     AGPLLGISLRKYPGNEQVEHEKYEAVIARAIDHMAEKYGAYPVFFPMQHPDDVPILEN
                     VASMMKRDSYVIKDKLDIFETYELISSMHMLLGMRLHALIFSAVATVPMVGLVYDPKI
                     QGFLDCIRQPSAGDVRELEYEKLADLMDSVWENSSEIRKQLSESIPGLKEKARENARV
                     AVELISGAKPEARPETGS"
     gene            complement(9482..9631)
                     /locus_tag="PLG72_04640"
     CDS             complement(9482..9631)
                     /locus_tag="PLG72_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOJ80120.1"
                     /translation="MFDALINGTDLEKGIFVTVFGMLGVFAVLILFYLIIKLFSGSFI
                     KKAEK"
     gene            complement(9658..10893)
                     /locus_tag="PLG72_04645"
     CDS             complement(9658..10893)
                     /locus_tag="PLG72_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ80121.1"
                     /translation="MDELLAGITSINIGMVVMYVIGLALIILAIKKEYEPMLLLPIGF
                     GAILVNLPQSVVKYASIASEPDMFVTNIIQNNPEAAGLFKNYIEIGGKYYKYEPGILQ
                     ILLESGILTELFPVLIFIGIGAMIDFSPLFKNPVMIFFGAAAQFGIFATMLVAIVLGK
                     FDLKTASAIGIIGAADGPTAIYVASRFAKEYFGPISVAAYSYMSLVPIIQPPVIRLLT
                     TKKERLIRMEYREEKFSKFVLIAFPIAVTIIAGIVAPISVPLVGSLMFGNLIRECGVL
                     ERLSQAAQNELSNLVTLLLGITIGSTMTAESFLRPETLLILAMGLVAFIFDTAGGVLF
                     AKFLNLFRKEGNKFNPMIGAAGISAFPMSARVVQKIAQKEDPTNFVLMQAVSANVSGQ
                     LGSIVAGGLVLALVPMLVH"
     gene            complement(11066..>11395)
                     /locus_tag="PLG72_04650"
     CDS             complement(11066..>11395)
                     /locus_tag="PLG72_04650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80122.1"
                     /translation="TADSFAGMIAGSVTGGAAWLRQLSIMPAHRRKGLGSRAVKLVFE
                     YLQQNFRAQEAFVSVVEENAAGLCFWNKLGFEAICSIEKELFESKQKHNVIIMRKTLR
                     NDKAEPL"
BASE COUNT         2517 a         2887 c         2856 g         3135 t
ORIGIN      
        1 tcgagaagat aaccggacgc aaatatgacc ggatccatat cgtgggcggc gggtcaggca
       61 acgccctcct gaacgccttt accgcaaact gctgcggaaa gccggtgtat gcaggccccc
      121 aggaagctac gaccctgggc aacggcatcg ttcagatgat atcgctgggc gaggtgtccg
      181 gttttaccca ggcaaggaga atagtaaaga acagtttccc gccggcggag tttatgccgc
      241 gtgatacccg acagtgggat gaggcttatc aaagatttat tgagatgatc aggaaataag
      301 gtgtccaatc atggcgttat gagaatagat agaagaacgg ttcccgtcag gacatgggga
      361 cggtccccgc gcctcatttc tccagagacg cggggaccgt ccccatgtcg tatgtctgta
      421 tgcctgttgt cgtgccaggt accctttggc ttatccttgt cttgcttcct ttatttttgc
      481 cgcgaactcg gcggccagtt tccttatgct tgcgtaatcg ccggttttgg cgcctgcggt
      541 gagacttccg ccggcaccga cagcgactgc tcccgctttg atccagtcac cgacgttgtc
      601 gatgctgacg ccgcctgtcg gcataaggtt agcctggggc aggggccctt ttatcgactt
      661 gattatgccg gggccgaagg cttctccggg gaataccttg agtatgtcgg ctccaagttc
      721 cattgcctcc acgacgccct cgatggtcat gacaccgggc atgcagggaa cctggtaacg
      781 gttgcagagc ttcagcgttt cccggctgag atacgggctc actacatact gggcgcccga
      841 aaggatcgct atcctcgctg tctcggcgtc gagcacggta ccagcgccga ttatgatttt
      901 accggcgtat tcttttgcaa gctcttctat tacttttgtg gctcccggta ccgtgtacgt
      961 tatttcaatc gctgcagcgc cgccctcaag gcacgcgtct gtgatccttt tcgccatgtc
     1021 ggcgttgtct gcccttacta ctaccaccag tcccgaatcg gtgatttttt tgagtatttc
     1081 ccactttttg aacatgtcag cctcctgtcg gtagtcataa tagagttgga ccgtacgtgt
     1141 cacttccatt tcgggttgtc gataataacc ggttttccat ccttttcagc aatcagcttc
     1201 ctgaagcctt ttgtttctat cgttccataa tcgtgtccgg cgtctgcgcg aaacacgaag
     1261 aaggttacca gtttttcgtc cccgatgttt atgctcctgt gggcatatct gccgggcaca
     1321 taaactgccc tgccgggggt cagaagctgc gcatcccagt ctccttcggg gttttccatg
     1381 agcatgtagc cttttccgct caggcagtaa tatacttcag cagtatcgag tatcgtatgg
     1441 aaatgccctt ttgtcatgaa atattcgttg ccgaccttgc cggggtacac gatactggtg
     1501 ccgaacagca ggttgcccga tgtctccggc aggccaagct catagaagtc gtatactatc
     1561 tcatcgtttg cgcctactgc tgcttcatat gcagcatcat ccgcatacat tcctctcatt
     1621 tgggaaagaa agcgcctggt gggttttctt tcgtcggaca tgcccgttat taagtcaaag
     1681 tacattgcaa aaggatcaac tttaatatca ttcattttta tcctcccagt cagttgatat
     1741 cgtggttcat gattcgctgc ccgtggctat agtctattga ccggtccata ataatcagcc
     1801 gttattgcgg acttcatcgg ctatcttttt gcattcgccg aaaagttccg gcgttatttc
     1861 aatatggaat acttcggcaa tgacctgggt ccagccgtgt atgaaatacg tccatccatc
     1921 ttctacatgc aggtttttag cctctttctg cgcaagagcc tggtgcatga agagcaggtc
     1981 gcccctgtag ttgatctccc agacgaggct gttttcgggg aaccgggcct catcggtcag
     2041 aggggaaccg ggcctgtctt tgccaaggcc tgtggcattg actatcagtg agtagggttt
     2101 cagcctgtgc agcagttcat cgttctggcc aggcccggga gtaagatgga attccgcgtt
     2161 ggcatccggc ctgatctgcc tgacgatgct ttctatatgg tcaagccttg gcttgctcct
     2221 gttgccgacg atcagcctgt ctggtatgtt gtcgcccttg tccatgttga gaaggtaagc
     2281 ggccatggcc actgcacttc cgccggcgcc cagcagcaag acatctccgc cgtgttcttt
     2341 gaagaaattc ttcggtacga acgcttcgag cgacagcccg gcggtgatgg gatccttggc
     2401 atatccttcg agccttccgt cttttttcga gatgcaggaa agttcgctga acatcacagc
     2461 atacggatcg agataatcaa acaggtcctt tgcggcatta tacaggtcga tcttgtgcgt
     2521 cgtgaccagc gcgccaagtg agagttcgtc gtttttgatg aaatcgacga cttccctgta
     2581 cacttcgggg tttgcatgta tctcgatgtc gatgcccttt ataaccgcgt ccttaaggcc
     2641 caatgccttt gcccacaacg ggaacacctt cattatcgag gatttcgttg tagtgacgcc
     2701 aatgaagtag aatgcaggtt gtgttgcttt tcctggtaag ttcatggtgt atctcctttc
     2761 attatcgttg ttctgtctca gatattgctt aagggaattt tcctttgcag aaaacctccc
     2821 gatccgtcag ttttaaggcc aggcggacag cagttgccat ccgccgggtt ataagctgtt
     2881 gcatgcgcac cacggacatc acgacggcag tcatgatgaa cgttatgttt tcttccggct
     2941 gaaggctttt gccgggttgc tcacggtaaa tttatatatg tgactgtcac tgacgccata
     3001 actcctgagc atggggaaga aggcctcgtg caggtagtcc agcccaatgg agcctccgta
     3061 acttttcatc ctttcgcggg tggtatcgag acccagcagg atcctgtcct cgaatccgtt
     3121 ctccaggaga cttattataa accttgcttc gtcatcatcg gagtggtact tgaacctgcc
     3181 tatggtgtcc agttccagat agacgccggt cgatgcgact tccgccaggt ggccggggtc
     3241 ggtgagcacc cggtcgagat ggcatattat tatggaactg acgggtactc cgtggtccgt
     3301 gaacagcctg atctgatcca gtgcgcccat ccccaactcc gtatggctca gtatgggagc
     3361 gccggtccgg acagatgctt cggcggctgc tgaaaacagt ctcctgtaat cgccggcagg
     3421 accttccatg tcggaggcag tcttgatgac gccggccctg ctttcgatcc tgcggacagg
     3481 ttttgaaata tcacagtcta taaacatgcc gttctccagt tcatcgataa aaagtccggc
     3541 gaggcagtct tcgtccatct tcctgatcca gtggctgtcg gggtagaaca cgagtttgtg
     3601 gaacccggtg gaagctatga tattcacgcc ggtccttaag gacgctttca actggagatc
     3661 ggccatacgg ccgcagccga caggctgagc gtcgactatg cttttgcccc cgatgctttt
     3721 gtacagttcc agctccgaac atgtggcatc gtagtcatcg agcctcagcg agggaaccag
     3781 ttttgcagag tatccgtccg caatgaagag atgctcatgg ctctggcagt atccaagccg
     3841 gtccggactg atacgtcccg tgacagtctg tatgatatat tcatcagggt gtgtctgcat
     3901 tctgttttct ttgcggttaa gaacgctttc catggctctg ctccttatga ccaagcatct
     3961 cgagaagatt cttcgtggaa accagcgcca ttttttcggt agcttcaccg gtaaatgcgc
     4021 caagatgcgg agtcagcagg aaattgtcca gcttgagcag tttttcatca tttgcaggcg
     4081 gttcgcttga gaatacgtcc tgtgcggctc ctgctatcct gcctgatacg agcgcttcgt
     4141 acagtgcatc ttcatcgacc agttcgcccc tcgatgtgtt gatgaggatg gcgtcgggct
     4201 tcatcaatcc cagggtccct gagtttatca tcttttttgt ctcgggtgtg gcagggaggt
     4261 gaagtgatac tatatcgctg gctgacaaaa tctcatcgat gctgccggcc ctgtttacct
     4321 tatactcgga gaggaaaccg tcgtccatga aatacgggtc gtaaacagag acttccattt
     4381 cgaggccccg tgcccttttt gcgacttcgc gccctatctg gccgcatccg atcagcccga
     4441 gtctctttcc tgtcagttcg aatccgatgc tgcgctccca gccgccgttt ttgacttcag
     4501 ctgcgtgaaa cggtattttc cgggccatcg tgaacatgag cgcgatcgca agctcagcga
     4561 cagaaacgct gtttgtcccc actgcgtttt ttacatttat gccaagctcg gcggcacgat
     4621 ccaggtcgat gttgtccata ccgacgccgt atttcgagat ggctttcagg tccctgagcc
     4681 gttcgagcac tgtaccgggc agcggatcga cgcctacgat tatccccacg acgccttctc
     4741 cggcaagttc cgttatctca ttttcggcca ttgtcctgcc aaaggtgttt tcgatgatcc
     4801 cgtagccctt ttcttcaagc agcggatatg ccttgtgctt gtagttcctg aatgattttg
     4861 gcgttatcag tatttttttc tccatgctta tccgtccttt ccacgtatag tctgcctctt
     4921 ttgctgctta tttagtgata tggcgccgtg ttttatgctc atcaaaatgt atttatgtaa
     4981 ttacataaat acataaaaaa tatatgcgtt cctatctgag ctgtcttttc agttcaaatg
     5041 taaatttgct cctgtcgccc ctgtacttag caagggaata ctccacgggg gtgccgtctt
     5101 ccaggtagac gatgctttcg aaatactgga tcggcgaacc cttttccact ttgagaagtt
     5161 ttgattcgaa ttcgcctgcc gttatacact ccagtgtcct gacggcgttg gatatgaggc
     5221 gtccgctttc cttttccatt atttcataaa gagactcgtt ttccaggtcg tgtcccatga
     5281 tcgggggaca gacgctgtaa ggtatatatg tgatgacgaa gactatggga tcatcatccg
     5341 cgaatctgag ccttgtgagc ttaattacgt cggtcccggg tccaaggcct aatgtttttg
     5401 ccaccttttc gtcgcttttt atgacctctt tctcaaggat ctttgtggag ggcacgagtc
     5461 ctttctttct catttcgttg ttgaagctgt ccagcgtcag cagaaaatcc tgcgttattt
     5521 tcggctttga cacgaatgtg cccttggcct tcaccctgta aaggtaacct tcaaccacaa
     5581 gctcgtttat agcctggcgg acagtaggcc tgctgatgcc gaaatactcg ctgatctcca
     5641 cttcggtcgg tatgggcttt tccaggtctt tgtgctcttc ccttatatat tcgagcagta
     5701 tcgtcttaag ctggtaatac agcgggatgg gtatgttttt attaagtgtt ttgtttccgc
     5761 cgagaaacat tttaaacctc gttatcttat cataagatat tgtatatgtt attatgtaat
     5821 tacattttac tcatacttat tatataacgt acgcacaaaa aagtccacac aaatttcatt
     5881 actgcatata cttcattggg catattataa aacagacgat cggcaggagg ggatccgatg
     5941 cagaagggga aagtcagaaa catgttttcc ggcggcaata cctcgaaagg ttttttcagc
     6001 aggttcgacc atatcatgag ccacgaggac gcccggagga tatttgtcat aaagggcggt
     6061 ccggggacag ggaagggtac cttcatgaaa aatatatctg agataatgac aaacaggggg
     6121 tatgatacgg agcacataca ctgctcgagc gacagcaaaa gcctggacgc actcgtcata
     6181 cctgaactga aagtggcgct gctggacggt actgctccgc acgtgatcga tccgaaggcg
     6241 ccgggagccg tcgatgagat cataaacctc ggtgaatact ggtgcggttc ggcactggcg
     6301 gaaaaaagag aagaaattat gaaaatcagc aatgaaataa gcgttttttt ccagagggct
     6361 tacaggtatc ttaaggcagc ccgccatata tatgaagaca gttcgatcct gtatggaagt
     6421 gcaatggaca aagcagggct taactccatt gcgggagaat tcaccgggat gctgttcgat
     6481 gtgtttccgc ctgcctgcaa accgggacgg cagcgctgcc tgttcgcaag cgccatcacg
     6541 ccggacgggc tggtcagcta tgtggacgac ctgatgactt tggacagtat atacgttttc
     6601 gagggtttcc cgggctccgg caccgatatt gtgctggaga agataaaaga ggccgctgtt
     6661 gaacgaggct ttaatgtcga agcgttttac tgcggttttg atccggataa gctggagcat
     6721 ttgataatac cggaactcaa tacgggattt tcgactgcag ggaaatacca ttccacagat
     6781 gcgtgtgtca acaaaagggt ggacttccgg gaactgctca acgacagtgc gatatcaaag
     6841 cttggcccgg aactggaata taatgatcat gaattcggca gtctcctcaa taaggccgtc
     6901 gaaatgatat caaatgcaaa aaaactccat gacgagcttg aagcatttta catacaaaat
     6961 atggatttcg aaggtatcag gaagaagcag gacgagacgg tgcagaagat cctcagctat
     7021 tcataatgtg cataatcaga agttaccggc tgcctccccg aaaactgtga gctgggccgg
     7081 gaacttgcgt gcaatacttg cgaacatgtc agcagcatta cattcatgac ccggtttccg
     7141 gcctggcttc cggtttcgcg cctgatatga gttccaccgc cactcttgca ttttcgcggg
     7201 ctttttcctt gagtcctggt atggattctg acaattgttt ccttatttcg ctgctgtttt
     7261 cccagacgga atccatcagg tccgcaagtt tttcatattc cagctccctg acgtcgcctg
     7321 ccgaaggctg acggatgcag tccaggaatc cctgtatttt gggatcgtag accagaccga
     7381 ccatgggcac cgtggctacg gcagagaaga taagcgcatg cagtctcatt ccaagaagca
     7441 tgtgcattga ggagatcagt tcgtatgtct cgaaaatatc cagcttgtct tttatgacat
     7501 agctgtccct tttcatcatt gacgcgacgt tttccaaaat aggcacgtcg tcgggatgct
     7561 gcatggggaa aaacacaggg tatgcgccgt atttctccgc catatggtcg atggcccggg
     7621 ctatgaccgc ttcatacttc tcatgctcga cctgttcgtt gcccggatat ttgcgcagcg
     7681 aaataccgag cagcggaccg gcgcctgcca gaccgagcct ttccatgatc ccggttttga
     7741 tgttttcagc cccgtccatt acggtgacgg cagcgtctgc agtcatcagg atcctgggtc
     7801 tgcttatctc aagagtttta agttcatcga aggacaaggc ttcccttaac gtgatcacgt
     7861 ctaccttgtt tatgatcttc ttcgtcagca gcctgttcat gctttttttc agcggaccga
     7921 tcccgtttgc atagaacatg accttaaggc cgagcctttt tgcgagccat accatgctga
     7981 gatagaacag gagcgatctt gtgctggtat tgtcctgcag caggttgccg ccgccgtata
     8041 tgaatgcttt cgattttctc attgccctgt agatgcggaa catgcctgcc ctgtctatcg
     8101 atgcgacgtt gaattccagt gcagttgcga ccggggtttt ggaaagaacc atgaggcgca
     8161 tgttcggcct gaagcttttc agatctttga ggatcgacat caggagggca tcgtcgccta
     8221 tgttcctgaa gccgtaatag cctgagatga tcgcgtcata atgcttgcgc cgagagcctg
     8281 actccgcttt tttctccagt atgctcctgt atatgtcaat ctgggtgcgg cccatggtct
     8341 cgacggagta tttggcataa gctttttcat agaggcgttc tcccatttca cgtcgctttt
     8401 tctcatcagc ggccagttcg aggatgagtt cggcgaaacg ttccacatcg ccgggtttga
     8461 acaggtagcc gtttgtcccg tgttcgatca ggtcagggat gccgccgacg tcggtactta
     8521 tggtagcctt tttgaaccgg gcgccttcca gtaccgaata tgcgaagctc tcgcttattg
     8581 aggtgaggac gctgatatct atgctgctca tcagttcatt ggggtcatcc tgccatccga
     8641 cgaagaacac cgtatcctga aggccaagct cggcagtcag ggactccagc ttttttctgt
     8701 cttcgccgtc gcccccgatt atgaatttta tcttcctgtt cctggctttt actatatgcg
     8761 cggccctgac aatggtctct atgttcttga cagggtagag ccttgcggcg ataccgacca
     8821 ggacttcgtc atcctttacg tcaagtctgt acttatccag gaacttcttc ctggaatact
     8881 gtacagaggg ctgcgaaaaa tccatgccgt tataaaggac atagatatca agcgggttga
     8941 atttcctttc gataagcatc tttctgaagt tgtcggaaac gcctatatgg ttttctatga
     9001 agcggagggc gatagaattg atcagcccta ttgtcagccg tttcggaagg ctgtgcatgt
     9061 aatcgagcct gtaatcgctg tggatggtgg tgactgtcgg tatgtcggtc ttccgcgtcg
     9121 cagccagcga aaatatattg gctttggcgc cgtgcgaatg gactatgtca taattgcctt
     9181 ccctgatgat ttttctgact ttgcggatat cattgtacat atttgcggat ttgaccacgg
     9241 tgacgtcgat gcccaatgct ctcgcatcgt cggcgaaaac accgggccgg aggctgatga
     9301 gctttacttc catgtggcgc gacagttctt ttagcaaata gagcatatgc gtttttgctc
     9361 cgcctacatc accgccgccg attatatgaa ctaccctcat acatacctcc atggactgac
     9421 tgccgcattt ctgcggcagt cagtatagtg tcagatatca gaatttccgt tcgtgaccgt
     9481 gctatttttc cgcttttttg atgaatgaac ccgagaacag ctttattatc aggtaaaaca
     9541 gtatcaatac cgcgaatacg ccgagcatcc cgaacacggt gacgaatatt cctttttcca
     9601 aatcagtgcc gtttatcaaa gcatcaaaca ttgttatatc ctcctcacat cactgcatca
     9661 gtgaacaagc atcggcacaa gcgcaagtac cagacctccg gctacgatcg agccaagctg
     9721 tccggataca ttggcactga cggcctgcat gagaacgaag ttggtagggt cttccttctg
     9781 ggcaattttc tggacgaccc ttgccgacat ggggaatgcc gatattcctg ctgcaccgat
     9841 catcgggttg aacttgttgc cttctttgcg gaaaaggttc aggaactttg cgaacaggac
     9901 accgcctgca gtatcgaata tgaatgccac gaggcccatg gcgagtataa ggagtgtttc
     9961 cggcctgagg aagctttctg ccgtcatggt tgaaccgatg gtgatgccaa gcagcagtgt
    10021 caccaggttg gacagctcat tctgtgccgc ctgtgaaagc ctctcaagaa caccgcattc
    10081 cctgataagg ttgccgaaca tgagggaacc gacaagggga acgcttatcg gggccacgat
    10141 acctgcaatt atcgtaactg ctatcgggaa agcgatcagt acgaatttgg agaacttctc
    10201 ctcgcggtac tccatcctta tcaggcgctc tttctttgtc gtaaggagcc tgatgaccgg
    10261 cggctgtatt atcggtacca gcgacatata cgaataggcg gctaccgaaa tcggaccgaa
    10321 gtactccttt gcaaatctgc ttgccacata aatggctgtc ggaccgtcgg cagctcctat
    10381 gatgccaatg gccgacgcgg tcttcaggtc gaacttgccc aggacgatag ccacaagcat
    10441 cgtggcgaaa attccaaact gggctgccgc gccgaagaat atcatcacag ggtttttgaa
    10501 aagaggcgag aaatcgatca tggcgccgat cccgatgaat atcagcaccg ggaaaagttc
    10561 cgtaagtata cctgactcaa gcaggatctg cagtatgcca ggttcatact tgtagtactt
    10621 tccgccgatc tcgatatagt tcttgaacaa acccgcagcc tcgggattgt tctgtatgat
    10681 gttggtcaca aacatgtcgg gttcgcttgc tatcgaggca tacttgacca ccgactgggg
    10741 aaggttaacc aggatggcgc cgaatccgat aggcagaaga agcattggct catattcctt
    10801 ttttatggcc aatattatca gtgccagacc gatcacatac attacgacca ttccaatgtt
    10861 aatacttgtg attccagcta gtagttcgtc catagtatct ctcccgcttc aaaatgttgg
    10921 ggtaaaagca atcgctgctc attaccatgc tatagtataa cttaaggttt tgcataaatc
    10981 aattaaaaat gtattatgtc gagttttgtt tgctgagaaa tttttctttt cccaggattt
    11041 tttctgcagc gggaaacacg gcctttcata atggctctgc cttatcattc cgaagagttt
    11101 ttcgcattat gatgacattg tgcttctgtt tgctttcgaa caattccttc tcaatgctgc
    11161 atatcgcttc aaagccgagt ttgttccaga agcacagacc ggctgcgttt tcctcgacaa
    11221 cggaaacgaa agcttcctga gcccggaagt tttgctgcag gtattcaaag acaagcttta
    11281 cggccctgct gcccaggcct ttcctgcggt gagcaggcat gatcgaaagc tgtcgaagcc
    11341 atgcggcgcc gccggtgaca ctgccggcga tcataccggc aaagctgtcg gctgt
//
LOCUS       DAOSCC010000058        11356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3819_length_11356_cov_4.480665, whole genome shotgun sequence.
ACCESSION   DAOSCC010000058 DAOSCC010000000
VERSION     DAOSCC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_11356_cov_4.480665"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1210
                     /gene="radA"
                     /locus_tag="PLG72_04655"
                     /pseudo
     CDS             1..1210
                     /gene="radA"
                     /locus_tag="PLG72_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907541.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
     gene            complement(1444..1839)
                     /locus_tag="PLG72_04660"
     CDS             complement(1444..1839)
                     /locus_tag="PLG72_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOJ80123.1"
                     /translation="MKDILIDEFQYTVQELIFRNKSIIDSITKYQDSNARVNRAIVKA
                     VTQCGCIRINAKKQEVPEDGSLEEIRKAMDTHLEGKLCDSCRDQIEKELGKNLYYIAS
                     LCNTLDLNLYDIIIKEQERVKMLGKYNLE"
     gene            2341..2820
                     /locus_tag="PLG72_04665"
     CDS             2341..2820
                     /locus_tag="PLG72_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOJ80124.1"
                     /translation="MFSVGDKVVYPMHGAGVIESIEEKEILGQKQRYYILRMPLGDMK
                     VMIPTQNVEGIGIREVIDSSAVSKVYEILQDHDVNVTNNWNKRYRENMSKIRSGNIYE
                     VADVVRILMKRDKEKGLSTGEKKMLNSAKQILISELILAENSEQVDVESMINEYLSE"
     gene            2884..3957
                     /locus_tag="PLG72_04670"
     CDS             2884..3957
                     /locus_tag="PLG72_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOJ80125.1"
                     /translation="MLNNIIKFSFATVGCITGITLTRTFFIYQLTDISPEIKIMAYIV
                     IALALGFIFYTTSNKLIEIVMESLDRLELFIQKMTLYEMMVGSVGLIIGLIIANLVTI
                     PIKNIDIIGTPVSIVVNILFGCLGVAVAAGKKNENIFESFRGRASDTSGKRRSDPKIL
                     DTSVIIDGRIIDICRSGFIEGELIVPSFVLDELRHIADSQDPLKRNRGRRGLDVLNMI
                     QKDLQFPMRIETMEVSSDSEVDNALLKMAQKLGGKVLTMDFNLNKVANFQGVEVLNIN
                     ELANAMKPIALPGEEMVVQVVKDGKENGQGVAYLDDGTMIVIDGGRKHIGEKLNVTVT
                     SILQTAAGRMIFAKPKYLAEREI"
     gene            4747..5478
                     /gene="ispD"
                     /locus_tag="PLG72_04675"
     CDS             4747..5478
                     /gene="ispD"
                     /locus_tag="PLG72_04675"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ80126.1"
                     /translation="MRRDESMQARGRGHVAAVIAAAGRGTRMDLDINKQYIEIHGMPI
                     LAMTISKFEECSFIDEIIVVANKDEIDYCRKNVIERFGFTKIRSVTEGGDSRQRSVYN
                     GLKQVSAGCGIVLIHDGARPFVSNDSIIECIEAAGKYGAACVAVPAKDTIKESDADGF
                     VIRTLDRSSLWQIQTPQAFAYEMIMDAHSRAARDMFEATDDAMLAEKYGHRVRLVMGG
                     YSNIKVTTKEDLAFAEVLFRRQHGN"
     gene            5620..6342
                     /locus_tag="PLG72_04680"
     CDS             5620..6342
                     /locus_tag="PLG72_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ80127.1"
                     /translation="MIEVTGLVKKFGDFTAVDNISFSVSKGEIFGLLGENGAGKTTTL
                     RMLATMLRPTSGHAVLKGYDTQKEPEKVRAQIGILFGGETGLYDRLTVAENITYFGEL
                     NGMSKDAIKERIRQLAQAFGMEDFVNKRASKLSKGMKQKAAFAHSIVHDPDIMLFDEP
                     TSGLDVTAARDVHDFILRCRSECKTIVFSSHTMSEVEKLCDRIGIIHKGKLIECGTVD
                     ELKKKHGNDNLEELFVRLVGAK"
     gene            6339..7553
                     /locus_tag="PLG72_04685"
     CDS             6339..7553
                     /locus_tag="PLG72_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80128.1"
                     /translation="MKLKHVMIVLRKEIKDLIRDRRTIISSIVIPMILIPLVNIVMGG
                     GIQKFEQDMNENVTVALSQSSNTAEIRELVRDRILSKNPNIILVDTDDPVKALENNEI
                     RCIIELESSYKEKLEEGKPIRVTLQYDESKSKSQAAADIVAWAVDEFSAEVIEERIIA
                     LGLDPAILQPVQIERMNIAPNDKGNNMMLEMMLPFLISMLVAIGGIPAATDLVAGEKE
                     RNTFEPLLTTMPDRGSLLLGKYLAVTLFSFVSLIAIMAGLSIGYMINPNSLTIGVDEG
                     LKGITLEPLAVVLALLITIALGMTFSGIQIALSTIAKSYKEAQTYLSFLMLATMIPGY
                     ATMFMQASDLSPVMFALPVMNTVVAFKMVLSGSINYFNLVLALATSIVFVALTLAFAA
                     SLFKKEKVLFRN"
     gene            complement(7705..8175)
                     /gene="ispF"
                     /locus_tag="PLG72_04690"
     CDS             complement(7705..8175)
                     /gene="ispF"
                     /locus_tag="PLG72_04690"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOJ80129.1"
                     /translation="MKVGIGQDSHRFDFEDTSKKLILGGVVFEGEPPLKGNSDADVIL
                     HSITNAISGVTCVNILGETADDLCLNKGITDSSVYLREALKHLEDCKIVHLSISIECK
                     RPKISPKINDIRKSLSALLEIPENCIGITATSGEELTPFGQGLGIQVISIVTVI"
     gene            8750..10189
                     /gene="proS"
                     /locus_tag="PLG72_04695"
     CDS             8750..10189
                     /gene="proS"
                     /locus_tag="PLG72_04695"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ80130.1"
                     /translation="MAQDKKFVEEITPMDQDFTQWYTDVIKKAELVDYSSVRGCMVIK
                     PYGYAIWENIQKELDQRFKQTGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGG
                     NEQLTERLCVRPTSETLFCDHYKNCIQSYRDLPKLYNQWCSVVRWEKNTRPFLRTLEF
                     LWQEGHTAHATAEEAQEETIRMLNVYAEFCENVLAIPVVKGRKTEKEKFAGAKATYTI
                     EALMHNGIALQSGTSHNFGDGFARAFGIQYTDKSNQLQYVHQTSWGMTTRLIGAIIMV
                     HGDDSGLVLPPRVAPIQVVVIPVSQHKEGVLEKASEITSRLSGIVRVKMDDSDKTPGW
                     KFNEYEMKGVPLRLEIGPKDIEKGQVVLVRRDTREKAFVPMDELEAKVTELLDSIHSN
                     LLEKATRLRNEKTYTAVDMSEFAKILETTPGFIRAMWCGDRACEDAIKEQTGATSRCI
                     PFDDKEPVSDKCIYCGKEAKTLVYWAKAY"
     gene            10580..11350
                     /locus_tag="PLG72_04700"
     CDS             10580..11350
                     /locus_tag="PLG72_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOJ80131.1"
                     /translation="MKRKIIILVAMAALLISTTCYATVAGSESGARPAFEKIESSTGE
                     VNVSVLNLREGPSTDYPLIGKLSKGQVLTVMGKLGDWYAVYDPDTGRVGAVYSKYLDV
                     EDASVPVSANAAEKTGKTSSPLPGKTVLTEDEQKLLELVNKAREENGLEPLAVDENLM
                     KVARIKAKDMVENNYFSHQSPTYGSPFDMMRQFDNTFKSAGENIAGNKTVEGAFKAWM
                     SSETHKKNILNPGFKVTGIGIENSPTYGKILVQQFIGR"
BASE COUNT         3154 a         2360 c         3169 g         2673 t
ORIGIN      
        1 ggtcagcctg gaagaaataa aggctgacac agagcagcgc tgccttaccg gaataaaaga
       61 gttcgaccgt gtgctgggcg gcggtgtggt aaagggatcg ctgatactgg tcggaggcga
      121 tccaggcata gggaagtcca cgcttctcct gcaggtctgc aacacgttgc agacggagtc
      181 gaaaatactt taggtgtccg gcgaggagtc ggcgagccag atcaagctga gggcggacag
      241 gctgaacgtt aagaggcgca acctgtttat agtttcagca tccagctttg aagcggtatc
      301 atcgatggtg gaggcggaaa aacccggact ggtcatcatc gattcaatac aagccatgta
      361 tacggatacc ataagctctg ctcccggcag tgtcagccag gtgagggaag tgaccgcgtc
      421 gctgatgaaa ctggcaaaga gcagcggcat cactgttata atagtaggcc acgtgacgaa
      481 agaaggggca atagccggac cgagggtgct tgagcacatg gtcgacacgg ttttgtactt
      541 tgaaggtgaa aggcatctta gctacaggat cctgcggact gtgaagaaca ggttcggttc
      601 gaccaacgag atcggcgtat ttgagatgag ggacaccggg ctggccgaga tatccaatcc
      661 ttcagagata atgctttcag gcagacagga aaacgttccg ggctctgttg tcgtttccag
      721 cctggagggg acaaggccca tgctcataga gatacagtcg cttgtctgtc ccacgagttt
      781 cggcgttccc aggaggatgg ccaccgggtt ggattacaac aggctgacaa tgctcatggc
      841 cgtcctggag aagagggtcg gactggcgct ttacaactac gatgcgtatg tgaatgtcgt
      901 aggagggatc aggctggatg aaccggcatg cgatcttggc atcgcggcgg ctgttgcatc
      961 cagtttcaaa aacatgcctg tggacgccgg ttatgtgctc gttggagaag taggtcttac
     1021 aggcgaggtg cgggctgtca gccagataga gaaaagaatt tcggaggcta tgaggctagg
     1081 gttcaagaac atcatcatcc ccgaaggcaa catgaaactg gcaggacaga tgaagacaag
     1141 gggagggttc ggcataatag ccgtctcaaa tatacaggag gcgctggaag tcgtttttcc
     1201 gggtgcatag gccgctgtat gtatagggtg gggtaatatt caaggatatt catgccgaag
     1261 acgcattcgg gtgtggctgt gctgaaggct gataaactat gtgaatgaac atggtcaacc
     1321 atgttccggt ccgattcagt caacctctct ttgaattacg ccaaccgcac tgaaaatcca
     1381 gccaatatag aaccggatgg agcctgaccc ccatccggtt caaaatcagg tttactcagg
     1441 atatcactcc aggttatatt tgccgagcat tttcaccctt tcctgctcct tgatgatgat
     1501 atcgtaaagg ttcaggtcaa gagtgttgca cagcgatgcg atgtagtaca ggtttttgcc
     1561 aagctccttt tcgatctggt ccctgcagct gtcgcacagc tttccttcga gatgcgtatc
     1621 cattgccttg cgtatctctt caagggagcc gtcttcgggt acctcctgct tcttcgcatt
     1681 tatcctgatg cacccgcatt gcgtcactgc ttttacaatt gcacggttaa ctcttgcgtt
     1741 cgagtcctgg tacttggtga tcgaatcgat gatgcttttg ttcctgaaaa taagctcctg
     1801 taccgtgtac tgaaattcgt caatcagaat atccttcata gcagttcact ctcctgatgc
     1861 gctgaatcct aatgcatagt gacttgaaaa tacataatca ggtaaatctt atgtatttta
     1921 gttcactata tattggaatc gaatagttaa aaatgtatca gcatgtttta attatagcca
     1981 tttggacaac ttcttgtcaa tgctgttgtt atattggcgt cccgttgtat tattggcatc
     2041 ggttcgtccg gtcattcgtg ataaggtgtc cttctgaaac ggaagaaggt ggtgaatcgt
     2101 ctcaaatgct gaaattctgg gctttgccat atgttttgac aataatacgc aattgtagta
     2161 tactttatgt tgtgagttgg caatgcttat cagcaacttc cttttatgta acaaattggc
     2221 aacgacaagt tttcactgat cgacgacgat aaagcttgat gttattcgcc gaacgattca
     2281 aacaaaagac aaatcacaga tgaaagtgat atttttttat ttcattctgg aggatttgtt
     2341 atgtttagtg tcggtgataa ggttgtttac cctatgcatg gagcgggagt gatcgagtcc
     2401 atcgaggaga aggagatact ggggcagaaa caaagatatt atatcctgag gatgcccctg
     2461 ggtgacatga aggtaatgat acccacgcag aatgtcgaag gtatcgggat cagggaagtg
     2521 attgacagca gtgcggtgag caaggtatac gaaatacttc aggaccatga tgtgaatgtc
     2581 accaacaatt ggaacaagcg gtatcgtgaa aacatgtcaa aaatcaggag cggaaatatt
     2641 tacgaagttg cggatgttgt caggatcctt atgaaaaggg acaaggaaaa agggctttcg
     2701 accggcgaga agaaaatgct caacagtgca aaacagatat tgatcagcga actaatactc
     2761 gcagaaaact ctgaacaggt tgatgtggaa tcgatgatta acgaatatct cagtgaatga
     2821 cgcagcaacg acgaggcaaa cagggaactt gggtagaacg atttcataag ggagttagat
     2881 aatgtgctga ataatattat taaattctct tttgcgactg ttgggtgtat aacggggatc
     2941 acgctgacaa ggacattttt catataccag ctgacggata tatcgccgga gatcaagata
     3001 atggcgtata tcgtcatagc tcttgcgctt ggtttcattt tctacacgac aagcaacaag
     3061 ctgatcgaga tagttatgga atctctcgac aggctggaac tgtttatcca gaagatgacg
     3121 ctgtatgaaa tgatggttgg ctcggtcggc ctgatcatcg ggctgattat tgcaaatctt
     3181 gttaccattc cgatcaaaaa catagatatc attggaactc ctgtgtccat agtcgtgaac
     3241 atattgttcg gatgtctcgg cgttgcagta gctgccggga agaagaacga gaatattttc
     3301 gaaagtttca ggggacgggc ttccgatacc tccgggaaga gaagatcgga ccccaagata
     3361 ctggatacga gcgtcatcat tgacggccgt ataatagaca tctgcagaag cggctttatt
     3421 gagggcgagc ttatagttcc cagcttcgtg cttgatgaac tgaggcatat tgcggattca
     3481 caggatccgc tgaaaagaaa caggggaaga cgcggcctcg atgttttgaa tatgatacag
     3541 aaggatcttc agttcccgat gaggattgaa accatggaag tcagttccga ttcggaagtc
     3601 gacaatgcgc tcctgaaaat ggcgcagaag ctcggcggca aggtcctgac gatggatttc
     3661 aacctgaaca aggtagccaa tttccagggg gtcgaggtac tgaacatcaa tgaactggcg
     3721 aatgcaatga aaccgatcgc cctgccgggt gaagaaatgg tggtgcaggt cgtcaaggac
     3781 ggcaaggaaa acgggcaggg tgtcgcgtat ctcgatgacg ggacgatgat agtgatcgac
     3841 ggcggacgca agcacatcgg ggaaaagctt aatgtcaccg tgacgagcat tctgcagacc
     3901 gcagcaggcc gcatgatatt cgccaagcca aagtacttgg ccgagaggga aatataacgg
     3961 caaaggctgg gggaagcaga taaagcggct ggaatgagat aatgcgatca tggtggctgc
     4021 aggggatgcg ggaattcgga ccgcaggtga taaagagtca gggccaccgg aaggatcaag
     4081 ggtagcgtaa aaagatacag caaccctctg ccctcgacca ctttgtagcc ggggactttt
     4141 ccgccgtcca gcatggtggt catggcctgc tccttgaccc gtttcagcca gaggctgatc
     4201 agcggctcaa tttgcaggat aactgtgagt aaggaatata aggtttcaga ccatgaagat
     4261 ctaaggccgg atgctcgggc agatctttga tgggctttaa ctaagaaaat tttgcaggca
     4321 acttaaaaca gaatggacat ttctcaattg agaaaatgct tctcaattga gattttttat
     4381 gccataatat ggagtaaatg gcttgccggc taaatatccg atctgtcaga gcacagagga
     4441 cggtacttct aaggtatcga cagtatgagc gccgtttagg agagtgccgg aaaagtgcaa
     4501 taacgcataa ggaaaagaca gacgagtact agcaagatat gacggagact cagatgggaa
     4561 gaaaatgcag agacagccgt tttcgatgga gagacagcct ggtgcggagc aaagacagat
     4621 actgtcatat aaaattcatg agtacgaggc agtatggcat acaggtaccg ggcaaaagtg
     4681 tgtcagcgtg ttcagagaga cagactgata atatgcaaag aagcatatgg caggtgcaga
     4741 gtgcgtatga ggagagatga atcgatgcag gcccgcggca gaggacatgt agctgcggtt
     4801 atcgcggctg caggcagggg aacgcgcatg gatcttgata tcaacaagca gtacatcgaa
     4861 atacatggca tgccgatcct ggctatgacg ataagcaaat tcgaagaatg cagctttata
     4921 gatgaaataa ttgtcgttgc caataaagat gaaattgatt actgcaggaa gaacgtcatc
     4981 gaaaggttcg gtttcacaaa gatcagatcg gtcacagaag gcggggacag caggcagcgc
     5041 tccgtttata acgggctgaa gcaggtttcc gcagggtgcg gtatagtttt gatacatgat
     5101 ggcgccagac cttttgtgag taacgacagt ataattgaat gcatagaagc tgccgggaaa
     5161 tatggcgctg cctgtgtcgc cgttccggca aaggatacga taaaagaatc agatgctgac
     5221 ggttttgtta taaggacgct ggacaggagc agcctttggc agatacagac cccacaggct
     5281 tttgcgtacg aaatgataat ggacgcccac agccgggcgg cgcgtgatat gtttgaagca
     5341 acggatgatg cgatgcttgc ggaaaaatac gggcacaggg tcaggctggt gatgggcgga
     5401 tactcaaata tcaaagtgac tacgaaagag gacctggcct ttgccgaagt cctgttcagg
     5461 cggcagcacg gtaactgatc atgaggcacc cggtcttcag gacccgggta ttataacgga
     5521 taataatgat ccaggcattg aaactgattc aggcatcaaa actgattcag atatatgacg
     5581 gaatattatg ttgcagattc gaggtgtgat atggaacaga tgatagaagt aacaggactt
     5641 gtgaagaagt tcggtgattt tacagcggtc gacaacataa gcttcagtgt cagcaaaggc
     5701 gagatatttg gactgctggg cgaaaacgga gctggcaaga ccactacgct gaggatgctg
     5761 gcaactatgc tgcgcccgac gtcgggccat gctgtgctca aggggtatga cacccagaaa
     5821 gagcctgaaa aagtgagagc ccagataggg atccttttcg gaggcgagac cggactgtat
     5881 gacaggctga cggtcgcaga gaacatcact tatttcggcg aactgaacgg aatgagcaaa
     5941 gatgccataa aagagcgtat ccggcagctt gcccaggctt tcggaatgga ggattttgta
     6001 aacaagcggg cttcgaagct ttcaaaggga atgaaacaga aggcggcgtt tgcgcactcg
     6061 atcgtgcatg atcctgacat aatgctgttt gacgaaccga cgtccggtct agatgtcact
     6121 gctgcgaggg atgtgcatga ttttatactg aggtgcagga gcgaatgcaa aaccatagtc
     6181 ttctcaagcc atacgatgag cgaagtggag aaactgtgtg accggatagg cattatccac
     6241 aagggaaagc tcatcgagtg cggtacggtc gatgagctta agaaaaagca cgggaatgat
     6301 aatctggaag aattattcgt caggctggtg ggtgcgaaat gaaactgaaa catgtgatga
     6361 tagtactgcg taaagagata aaggacctga tccgtgacag gaggacgatc atatcgagca
     6421 tagtgatccc gatgatactg atacccctcg ttaatatcgt gatgggcggc ggtatccaga
     6481 aatttgagca ggatatgaat gagaatgtca cagttgcatt gtcgcagtcc tcgaatactg
     6541 cggagatcag ggaactggtc agggacagga tattgagcaa aaaccccaat attatccttg
     6601 ttgatacgga cgatcctgtg aaggcgctgg agaataacga gatcaggtgc ataatagagc
     6661 ttgaaagctc gtataaagag aaactggaag aagggaaacc gatccgggta actcttcagt
     6721 atgatgaatc caagagcaag tcgcaggccg cagcggatat cgttgcctgg gctgtagatg
     6781 aattcagtgc ggaagtcatc gaagagagga taattgcttt aggcctggat ccggccattc
     6841 tgcagcctgt acagatcgaa cgcatgaata ttgctcctaa tgacaagggc aacaacatga
     6901 tgctggaaat gatgcttccg ttcctgattt cgatgctggt cgccattgga gggatacctg
     6961 ctgcaaccga tcttgtagca ggagaaaagg agcgcaatac ttttgagccg cttcttacaa
     7021 ccatgcctga cagaggatcg ctgctgctcg ggaaatatct cgcagtaaca ctgttttcct
     7081 ttgtcagcct tatagctatc atggcaggcc tatctatagg ctatatgata aatcccaatt
     7141 ccctcactat aggagtggat gaaggattga aaggcattac gctggagccg ttggcagtcg
     7201 ttctggcgct gcttataacg attgccctcg ggatgacgtt ttcgggcata cagatcgcgc
     7261 tcagcaccat cgcgaagtca tataaggaag cccagacata tctgtccttc cttatgctgg
     7321 cgaccatgat acccggatat gcaacgatgt tcatgcaggc aagcgacctt tcgcctgtca
     7381 tgtttgcgct gcctgttatg aataccgttg ttgcgttcaa aatggtcctg tcaggcagta
     7441 taaattattt caacctggtg ctggcgttgg ctacatcgat cgttttcgtt gcgctgacac
     7501 tggcgtttgc agcgtcattg ttcaagaaag aaaaggtatt gttcaggaac tgatgcagga
     7561 tatgctgcgg ccgtacctgc gtgaaagata acgaataaac gtatttctga aggaagagca
     7621 cgttaatata taaaacggtt tcgacggccg gcggtccgga ctctgtttcc cggatctgcc
     7681 ggccctgatt tgaagctttc cggcctagat caccgttacg atacttataa cctgtattcc
     7741 caggccctgg ccgaacggag tgagttcttc gccgctggtt gccgttatgc ctatgcagtt
     7801 ctcggggatc tccagcagtg ccgacaggct tttccttata tcgtttattt ttggcgatat
     7861 tttgggcctt ttgcattcta ttgatatgga cagatgcact attttgcagt cttccaggtg
     7921 cttgagcgct tcacggagat agacgctgct gtctgtgatt cctttattca ggcacaggtc
     7981 gtcggctgtt tcgccgagga tgtttacgca ggtgactcct gatatcgcgt ttgtaattga
     8041 atggaggatc acatcggcgt cactgttgcc tttcagggga ggttcgccct caaaaaccac
     8101 accgccgaga atcagtttct tgcttgtatc ttcaaaatca aacctgtgac tgtcctgccc
     8161 gatacctact ttcatttcaa caacctgcct tccggctgca gtgagccgat ttttgaaccg
     8221 gtccaaaggt tcggcatatg ccgggtatag tccaaatgta tgccaaacgc gcgttacaca
     8281 ctagcattat attgccggat gcatgttttg tcaaagagta cggctggtaa ggggtaagtt
     8341 ttccggcttg ttgcaataag ttcgctgtcg tatatttcgg ggctgaaatt actttttcaa
     8401 atgaacctgc caactagttg caataagtct gccggcctgc tgcgggagct tgctgctgga
     8461 ggtattgaca ttgccgcatt tggtgtatta tactacttca taaatagcgc aaacgtcgat
     8521 gacgggaatg cgaaaggcag tacctttctt cccacccctg agatgcagcc gatgaggttg
     8581 ccgtgtgagc tgcgatacag gctcgacgat tctgtttcct tatatggaag ccggatctga
     8641 gtggggattt cccaataagg gtggtaccgc ggagatgatt cgtccctgtg ttgcaggggc
     8701 gttttttatt tgctgctgaa gttcaatact cgttaaaagg agtggaaata tggcacagga
     8761 caaaaagttt gtcgaagaga taacgccaat ggaccaggac tttacgcaat ggtacacgga
     8821 cgtgattaaa aaagctgaac tggtggatta ctccagcgtg aggggctgca tggtcataaa
     8881 accttacggg tatgcgatct gggagaacat ccagaaggaa ctggatcaaa ggttcaagca
     8941 gaccgggcac gagaacgttt atatgccgat gttcatacct gagagcctgc tgcagaagga
     9001 gaaagaccac gtggaaggat ttgcaccgga agttgcatgg gtgacccacg gagggaacga
     9061 gcaactgacc gagaggctgt gcgtcagacc gacttcagag acgctgttct gcgaccatta
     9121 taagaattgc atacaatcgt acagggacct tccgaaactg tacaaccagt ggtgctcggt
     9181 ggtcaggtgg gagaagaata ccaggccgtt cctcaggaca ctggaatttc tgtggcagga
     9241 agggcatact gcacatgcta cggctgaaga agcccaggaa gaaactatca ggatgctcaa
     9301 tgtctatgcc gagttttgtg aaaacgtgct ggccatacct gtggtaaagg ggcggaaaac
     9361 cgagaaagag aagttcgcag gcgcaaaggc gacatacacc atcgaggcgc tgatgcacaa
     9421 tggaatagcc ctgcagtcgg gcacatcgca taatttcgga gacggatttg caagagcgtt
     9481 cggcatacag tacaccgata aatcgaatca actgcagtat gtgcaccaga catcctgggg
     9541 aatgaccacc aggctgatag gcgcgatcat catggtgcat ggcgatgaca gcgggctcgt
     9601 gctgccgccc agagtcgcac cgatacaggt ggttgttatt cctgtttcac agcacaaaga
     9661 gggtgtcctt gagaaggctt ctgaaataac ttcgagactg tccggcattg tgagagtcaa
     9721 aatggacgac agcgacaaga ctccaggctg gaagttcaac gaatatgaga tgaagggggt
     9781 tcctctgagg ctcgagatag ggccgaaaga tatcgagaaa ggacaggtcg tgctggtcag
     9841 gcgtgacacg agggaaaaag cgttcgtgcc gatggatgaa ctggaggcaa aagtgaccga
     9901 actgctcgat tcgatccaca gcaaccttct cgaaaaagcg accaggctga gaaatgaaaa
     9961 aacgtatact gcagtggata tgtccgaatt tgcgaagata cttgagacca cgccgggctt
    10021 tatcagggcg atgtggtgcg gagacagggc atgtgaagac gctataaaag aacagacggg
    10081 cgccacttcc aggtgcatac ctttcgatga taaggaacct gtttcagaca aatgcattta
    10141 ctgcgggaag gaagcaaaga cgctggttta ctgggccaag gcgtactagg cgggcaaaac
    10201 gggaaaacgg aagagtggtt tgggtcggtt aaccggaaga catgaaagca gaaaagatcg
    10261 attctgtgac cgaaaggagc ggagccggaa aggcaggtaa tgtgcagccg gtaagcatgc
    10321 agtttggtaa gcagactgac tcaccggaca gaaaacgtgt ataaggggaa atcaggcgaa
    10381 ttcatcagat agaatacatg cgtcggaaga cagacgagtt cggcggcagg aaagagcgca
    10441 gccggaaagg aaggcagggg gcctgaaaaa atactaccaa attgccactt aaatgcaaat
    10501 aattcaaaaa tatttgtcaa tactatattc agcataatta ttaaaaaatt tgcattaaga
    10561 ggtaattgga ggtaggagta tgaaaagaaa gatcattatc cttgttgcaa tggccgcatt
    10621 gctgatttcc accacgtgtt atgcaacggt tgccggcagt gaatcagggg ctcggccggc
    10681 ttttgagaag atcgagagca gcaccggtga ggtaaatgtt agcgttttga acctcaggga
    10741 agggccttcc accgactatc cgctgatcgg caagctgtcg aagggacagg tgcttactgt
    10801 aatgggcaaa ctgggcgact ggtatgcagt atatgacccg gatacaggcc gggtcggcgc
    10861 agtttacagc aagtacctgg atgtcgagga tgcttccgtg ccggtttcag cgaatgctgc
    10921 tgaaaagaca ggaaaaacat cttctccgtt gcccgggaaa acagttctga cagaagatga
    10981 gcagaagctg ctggaactcg tcaataaagc gcgggaggaa aacggccttg aaccccttgc
    11041 ggtagatgaa aacctgatga aagtggcaag gataaaggcg aaggatatgg tagagaacaa
    11101 ctacttctca caccagtctc cgacttacgg ttcaccgttt gatatgatga ggcagttcga
    11161 caatacattt aagtctgccg gtgagaacat agccgggaac aaaactgtgg aaggcgcatt
    11221 caaggcttgg atgtcttctg agacacacaa gaagaacata ctgaacccgg ggttcaaagt
    11281 gaccggcata gggatcgaga acagcccgac ttatgggaaa atcctggtcc agcagtttat
    11341 tgggagataa tatcgt
//
LOCUS       DAOSCC010000059        11263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3848_length_11263_cov_4.472520, whole genome shotgun sequence.
ACCESSION   DAOSCC010000059 DAOSCC010000000
VERSION     DAOSCC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3848_length_11263_cov_4.472520"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..233
                     /locus_tag="PLG72_04705"
     tRNA            158..233
                     /locus_tag="PLG72_04705"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Ala,seq:ggc)
     gene            complement(615..1955)
                     /locus_tag="PLG72_04710"
     CDS             complement(615..1955)
                     /locus_tag="PLG72_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80132.1"
                     /translation="MRSEKLMDYIGQIDDSFIIEADSQPANTKSARRPRIKWVPAAIA
                     AACLIISSAFLLILMRPSGPVDLPDLPKLTVNTDLGSFGFEGLMAYNIDELRNGNPWT
                     ENNDLETMPVFANPHEYDSAGAPVSGLTPEEMLAEAEKIADIFGLDVTSLYTEPALEE
                     IEQIIQKMEAIGASEEEISRNTSIFYAAAKCNGAEIRVEKDGHVYLSLTPETAHLAEE
                     IEKLSAYESFEVSFDIKYKTEDGTMYSTGLPLPAGYSFTFSNTSNEQALEITQYLYSK
                     YGAFTGITTPGFDVAADYTFSGVLTRPHTFVFENEGTLTDRILNYNFRRLYFYATDSG
                     GLGGISYYNADLSKKIGDYPIITAEEARRLLLEGHYITSVPEALPGKRYIAHVELIYR
                     AGRLDTVFMPYYRFLVELPSMELENGLKTYGAFYVPAVRSEYLENMPVWDGSFN"
     gene            complement(1952..2512)
                     /locus_tag="PLG72_04715"
     CDS             complement(1952..2512)
                     /locus_tag="PLG72_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ80133.1"
                     /translation="MDDEMILDLYWARSESAISETANKYGSYCTKIAVNILQNSEDAE
                     ECVNDTYHKAWDSIPPQRPANFRAFLGRITRNLSLNRYKEQRTQKRGGDNITLIYSEL
                     EECIPSNSNVETEYESGLVAGAINSFLLTLDKESRIVFVRRYWYADPIQAIAERFGMS
                     ESKVKSMLFRTRKKLRAYLENEGVIL"
     gene            2685..2996
                     /locus_tag="PLG72_04720"
     CDS             2685..2996
                     /locus_tag="PLG72_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOJ80134.1"
                     /translation="MEFRKIFDTIPEQFDKYRPRYCAELFDDLIEYAGIGPGKTVLEI
                     GPGTGQATDPILDTGCGYHAIELGENLCEMMKKKYGKYPNFNIVNGDFITYDFGSKMD
                     M"
     gene            3168..3836
                     /locus_tag="PLG72_04725"
     CDS             3168..3836
                     /locus_tag="PLG72_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ80135.1"
                     /translation="MTCFEVNPGESVGIVGPSGVGKSTIAELLLGFYEHQEGIITIDG
                     YDIRKVRISDLRNHIGFVSQDVFLFSGTILENIRYGFISASDEQVIEVAKAADAHDFI
                     CSLPHGYYTIVGNRGFKLSAGQRQRISIARALLKNPEILIFDEATSALDSSSQMIIQK
                     TLNDFFKGRSRLIIAHRLSTVSNCDRIYVLNEGRVAESGTHIELLEKKSLYWELYSKE
                     AGIA"
     gene            3931..4314
                     /locus_tag="PLG72_04730"
     CDS             3931..4314
                     /locus_tag="PLG72_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ80136.1"
                     /translation="MNDDISITDSEWLVLKVLWENSPQTLPQIAEKLSYTGWSISTIQ
                     TFLARLVKKSAVSTKKQGKGFLYYPAVKEEDCQLVQARRFIDRVYDGSLSSMVMGVLK
                     SGSLTREEFERLKKLVEDFERSKKQ"
     gene            4311..5258
                     /locus_tag="PLG72_04735"
     CDS             4311..5258
                     /locus_tag="PLG72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ80137.1"
                     /translation="MIADIFYIMLFISCVSAISWLMLLFVRQVGNIRLPHAFYVILML
                     LLVIPVKYPGLKLIDPENDFMPGVGIAARIWIAGMIAYAVFMILKMLILKLSVRKFTR
                     CTDPDILALLDVCAKQAGLKKSPVVYYGTLNEPACVATCIRPAVVLSQKVTARLSEEE
                     LFMVLLHECIHVKRFHLPLKRLFTLLCCIHWMNPVIWIAKRAFSLSCEMDCDAHVIKR
                     LPSGSEAGYARMLMRLMEMSRRKPLALRDSAGILAYREMKQRFEKLFLPAAGVRRISA
                     VIACVLMLIVTVWCSLSISKGTFYPYPAYQNSSMEWSER"
     gene            5259..6131
                     /locus_tag="PLG72_04740"
     CDS             5259..6131
                     /locus_tag="PLG72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80138.1"
                     /translation="MPVIDINNVTKIYKNGVVGLEDVTFSIGPGVFGLLGHNGAGKTT
                     LMRMIATILRPTSGTITVMGHDTVKEGEAVRRNIGYLPQELSMYANLTALEFVSYMAR
                     LKGVTNAEEALNKLDEVGMAEYRNRKIGQLSGGMKRRVGIAQALVGNPRIVIVDEPTA
                     GLDPEERVRFRGILSRFAGNDRTVILSTHIIEDVHQICSHLAVLRKGRLFFQGPSDSL
                     LDRVRGKVKVVELSSDRDLADLRKRAVVISTTYGSKSLTVRIMDENGRFPQAPVHETL
                     EDAYVYCMGGNEHA"
     gene            6124..6879
                     /locus_tag="PLG72_04745"
     CDS             6124..6879
                     /locus_tag="PLG72_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80139.1"
                     /translation="MRNIATILKYEISMQIKSVRLWVVFAFALAVALLDNFPSEANLD
                     RLDFLTDQGYVVSRILAHAGVLMLFGFMFLLSDRIRGDRKKGISELFMASPLSKKQYI
                     WGKVLGNYVTVLFTMAIYFAINALVQLVHNPGPFSPVPYAVGFFAMAVPACFFVSACA
                     VALPAVIDIRMFYVLFSIYFLMNTDFIYTENGMIRSLYLFQKDILKLVYTYKQFEQII
                     PAGLLWNLAFLICSGIGAILLLKCRKSIWRETA"
     gene            7118..7528
                     /locus_tag="PLG72_04750"
     CDS             7118..7528
                     /locus_tag="PLG72_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80140.1"
                     /translation="MGYLQILCRVRHWQHALYTLHFSVLFPDASAIEMLIVYIGTSFF
                     LSTLGVLVSFLSRQPHLPAVVCGIAWLLSLLLRAAFMENLVRLPVIGCFYPFIRFAAP
                     GSSLWVMNKAVLFLLGAGMWAGVYFVCRKRKLML"
     gene            7881..8555
                     /locus_tag="PLG72_04755"
     CDS             7881..8555
                     /locus_tag="PLG72_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ80141.1"
                     /translation="MVRILVVDDDKNTRRLLEAVLTAENYTVFTASNGEEALEVMDRE
                     HIDLVVLDIMMPKMDGYEFTRVLRESNNNLPILMVSARQLPEDRHKGFIVGTDDYITK
                     PIDVEEMLLRIKALLRRAQIATEKKIVIGDVVLDYDSLTVTKNGDVQELPQKEFMLLF
                     KLLSYPGKIFTRIQLMDEIWGNDSESGWETVTVHIARLRKRFEGWEEFEIVSVRGLGY
                     KAVKKV"
     gene            8590..9606
                     /locus_tag="PLG72_04760"
     CDS             8590..9606
                     /locus_tag="PLG72_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ80142.1"
                     /translation="MLFSGVVFSFFVVTMIVVGGVIIYLIQRGILEPVGQGRRGHHLI
                     FRMMIASVMIGSILSAVASRIPLRPVNAIINAMNRLASGDFKTRLSFGKYLSKHPTAK
                     EISRSFNNLAEELERTEILRSDFINNFSHEFKTPIVSIAGFAKLLKHGNLTEDQKAEY
                     IDIIEEESMRLAAMATNVLNLTKVENQTILTGVTEFNMSEQIRNCFLLLENKWTKKNI
                     TPELDFGEHTIRANEEMLKQVWINLIDNAIKFGDEGSTVRVTITETEKNIFVSISDTG
                     VEIPMELRERIFQKFYQADESHSGEGNGVGLAVVKEIVRLHNGDVTVSSGNRKTVFTV
                     SLPK"
     gene            9836..10258
                     /locus_tag="PLG72_04765"
     CDS             9836..10258
                     /locus_tag="PLG72_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80143.1"
                     /translation="MRNWFGNLKYRLQQFMYGRYGYDELSRFVSVLGLVLLVLSLVPY
                     LRILYCFALILLIWTWYRAFSRNVYRRQAERQKYMELKYRVTQRFSLWRNMWRDRKTH
                     KYYRCPYCKATVRIRRPGRGKTISISCPKCVREFSKKT"
     gene            10504..>11263
                     /locus_tag="PLG72_04770"
     CDS             10504..>11263
                     /locus_tag="PLG72_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ80144.1"
                     /translation="MRKSIGIDLGTTNSCVAVVEGGKPVIIPNENGNRTTPSVVAFTK
                     DGERLVGDQAVRQAVINSERTIRSVKRHMGTGWKKEIDGKPYSPQEISSMILRKLKAD
                     AEAYLGEKITDAVITVPAYFNDIQRQATKDAGKIAGLNVMRIINEPTSAALAYGLDNG
                     TPQKIMVFDLGGGTFDVSIIEIGEGVIEVLATNGDNHLGGDDFDERIISHLADVIQKE
                     HHVDVRRDFAALQRVKEAAEQAKKELSFSETSYIA"
BASE COUNT         2917 a         2500 c         2839 g         3007 t
ORIGIN      
        1 tctattgttc tgccagatta tccctttaaa ttccctacag taactttacg ctaacttggc
       61 atgtccaggc agaaaattac attctaaaat ggctgttatt atgccttgac tactggggcc
      121 gtgatatatt aaaattagta tacgcctgaa gaaacgtggg gcattagcgc agttgggagc
      181 gcatcacact ggcagtgtgg gggtcgaggg ttcgagtccc ttatgctcca ccaaatctga
      241 taaccaggat agatacgaca agagccagtc catcagggct ggctcttgtc gtatctattt
      301 tcatttgcag aaagcattct tttattgata ttttggagca actgcagcat ttcggcatcc
      361 aatatgataa atttcaattt acctttccat atactcttat tattttttat tattcaataa
      421 gttcccgata ttgtttgcaa gattacttac cgtttccaat ccccaggtgt cctttgaagc
      481 ctctcccata atcgttcctc cgctgtgaca ataaccggga ccgtcgctga cgacctggaa
      541 gttcagctgc agcagaaaag tactaatata aaagcatttg agactgtttt ttgccgtgcg
      601 gtttattcgc tggtctaatt aaaactgccg tcccataccg gcatattttc aaggtactcg
      661 ctccggacag ccgggacata gaatgcaccg taagttttca gcccgttctc aagttccatc
      721 gacggcaatt ccaccaggaa cctgtaatac ggcataaaca ccgtatcaag cctgcctgcg
      781 cggtagatca gttcaacatg ggcaatgtac cttttgccgg gcaatgcttc cggaacggat
      841 gttatataat gaccttcaag caggagcctt cttgcttctt ccgcagtgat tatgggataa
      901 tcgccgattt tcttcgacag gtcggcattg taatacgata tcccgccaag tccgcctgaa
      961 tcggttgcgt agaaataaag cctcctgaaa ttgtaattga gtatacggtc cgtaagggtg
     1021 ccctcgtttt cgaagacgaa agtgtgcgga cgtgtcaaaa ccccgctgaa tgtataatct
     1081 gctgcaacgt caaaaccagg tgttgttatt cccgtaaaag cgccgtactt tgaatacagg
     1141 tattgtgtta tctccaacgc ctgttcattg gatgtgttgc tgaaggtaaa actgtatccc
     1201 gcaggtaatg gaagaccggt actgtacatg gtgccgtctt ctgttttata tttgatatcg
     1261 aaagagactt caaagctttc ataagcactg agcttttcga tttcttcggc aagatgagcc
     1321 gtttccggag tcagtgacaa ataaacgtgt ccgtcctttt caacccttat ttccgcgcca
     1381 ttacattttg cagctgcata aaaaatgctt gtatttcggc ttatttcctc ttcggatgcg
     1441 ccgatggcct ccattttttg aatgatttgc tctatttctt ccaaagcagg ctctgtgtac
     1501 agcgatgtta catcaagacc aaaaatatca gcgatctttt ctgcctcggc aagcatctct
     1561 tcgggcgtca ggccgcttac cggcgcacct gcactgtcat attcatgagg attggcaaac
     1621 acaggcatgg tttcgaggtc gttgttttcc gtccacgggt tgccattccg cagttcgtct
     1681 atgttatatg ccattaaccc ctcgaagcca aatgacccca aatctgtgtt cacagtgagc
     1741 ttaggcagat cgggcaaatc gaccggtccg gatggccgca tcaggataag caaaaacgct
     1801 gaagaaatga taaggcaggc agcggcaatg gcggcaggca cccatttgat ccggggccgc
     1861 cttgcggatt tggtatttgc cggctggctg tctgcttcta taatgaaact gtcatctatc
     1921 tgaccgatat agtccatcag tttttcggat ctcacagtat cactccttca ttttcgaggt
     1981 aggccctgag ctttttgcgt gtccggaaga gcatggattt taccttgctc tcgctcattc
     2041 caaaacgctc ggcgatcgcc tgtatgggat cggcatacca ataccgcctt acgaatacga
     2101 tcctgctctc tttgtccaac gtcagcagga aagagtttat tgcaccggca acaaggcccg
     2161 attcgtattc cgtctcgaca ttgctgtttg acggtatgca ttcctccagt tcggaatata
     2221 tcagcgtgat attgtcgccg ccgcgttttt gggtcctctg ctccttgtac ctgttgagcg
     2281 aaaggttgcg tgtgatcctg cccaaaaacg cgcggaagtt tgcagggcgc tgcggaggta
     2341 tggagtccca cgctttatgg tatgtatcat ttacgcattc ttctgcgtct tcgctgtttt
     2401 gcaggatgtt gaccgctatt ttagtgcaat agcttccgta tttgtttgca gtttcgctga
     2461 tagccgattc ggagcgcgcc cagtacaaat cgagtatcat ttcgtcatcc atctcaataa
     2521 accccttgta tttttttgaa gcttcaccag taaagacaac aaaaaagtgg atcggttgca
     2581 taataaggca aaaaattcta tcaatccgga tacaaaaaca ttatactata tttaggacac
     2641 gggcatgtcc gcagttcggt tgtttcagaa aggaacgaaa tgtcatggag ttcaggaaaa
     2701 tattcgacac tatacccgaa cagttcgata aatacaggcc gcgctactgc gccgaactgt
     2761 ttgacgacct gatagagtat gcgggaatag gtccgggtaa gacggtactg gagatagggc
     2821 cggggaccgg gcaggcaacg gatcccatat tggacaccgg atgcggttac catgcgatcg
     2881 agcttggtga aaacctgtgt gaaatgatga aaaagaagta tggaaagtat ccgaacttca
     2941 atattgtaaa cggtgacttc attacatacg acttcggcag caaaatggac atgtaatttt
     3001 ttgatcaatt gtgataattt cagcttccat attgagttat tctcaagggg caaacattct
     3061 tacaccgagt gcattggata ttgtcattag cgatacaact tctatttatg ctacgctttt
     3121 gtgaaactaa ataatcctta tctttgtgac ctaatttata taaatcaatt acatgttttg
     3181 aagttaaccc gggggaatct gtcggcattg tcggacccag tggtgtcggc aaaagcacca
     3241 tagcagaact gctacttgga ttctatgaac atcaggaagg gattattacc attgacggtt
     3301 atgacattag gaaggtcagg atatctgatt taaggaacca cattggtttt gtcagccagg
     3361 atgttttcct tttcagcggg acgatactcg agaatatacg ttacggtttc ataagcgcat
     3421 ctgacgagca ggtcattgaa gttgcgaaag ctgccgatgc acacgacttc atatgctcgc
     3481 ttccacatgg atactatacg attgtgggga acagaggatt taaactttcc gcaggacaga
     3541 gacaaaggat ttcaattgcc agggccttat tgaagaatcc ggaaattttg atttttgatg
     3601 aggctacatc tgctcttgac tcaagttcgc aaatgattat acaaaaaacg ctgaatgatt
     3661 ttttcaaggg aagaagccgc cttatcattg cacacagact atccaccgta tcaaactgcg
     3721 ataggatata tgttcttaat gagggcaggg tggccgaatc cggtacccat attgagcttc
     3781 ttgaaaaaaa gtcgctatat tgggagctct attcgaaaga agcaggtatt gcttaatggt
     3841 attgcttaat agtgattatc caaaatattc attgacaaat gtaggtcaaa actatataat
     3901 aatagcaaat gttggttacg gaggtaatat atgaatgatg atatatcgat aacagattcg
     3961 gaatggctgg ttttaaaagt actttgggaa aattcacctc aaacgctgcc gcaaattgcc
     4021 gagaaacttt cgtataccgg ctggagcata agtacgatcc agactttcct ggcacgtctt
     4081 gtaaaaaaat ccgccgtatc cacaaaaaaa cagggtaaag ggtttttata ctatccggcc
     4141 gtaaaggagg aggattgcca gttagtgcag gccaggcgct ttatcgaccg cgtatatgac
     4201 ggctcactga gcagcatggt aatgggtgtg ctgaaaagcg ggagtcttac ccgggaagag
     4261 tttgaacgct taaagaaact ggtggaagac tttgaaagga gtaaaaagca atgattgcgg
     4321 acatattcta cataatgctg tttataagct gcgtcagcgc gatatcgtgg cttatgctcc
     4381 tgtttgtgcg gcaagtcggg aatatcaggc tcccccatgc gttttacgta atattgatgc
     4441 tgttacttgt gattccggtc aagtatccgg gacttaaact gattgatcct gaaaacgatt
     4501 ttatgccggg agtcgggatt gctgcgagga tatggattgc cggcatgatt gcttatgccg
     4561 tgttcatgat cttaaaaatg ctgattctta aattatctgt gcggaagttt acccgttgca
     4621 ctgacccgga tatactggca ttgctcgatg tctgcgcaaa gcaggcaggt ctgaagaaaa
     4681 gccccgttgt ctattatgga acgctgaacg agcctgcatg tgtcgcgacc tgcatacggc
     4741 ctgccgttgt attgtcccaa aaggtgactg caaggttaag cgaggaagag ctgttcatgg
     4801 tcctgctgca tgaatgtatc catgtgaaac ggtttcactt gccgttaaaa aggttgttta
     4861 ctttactttg ctgcattcac tggatgaacc ctgtgatatg gatcgcaaag cgcgcttttt
     4921 ccttatcatg tgaaatggat tgtgacgcgc atgttataaa gaggttgcct tcaggcagcg
     4981 aggccgggta tgcacgcatg ctgatgagac ttatggaaat gtcccgccgt aagcctttgg
     5041 ccctgcggga ctcggcaggc atcctggcct acagggaaat gaagcaacgg tttgaaaagc
     5101 tgttcctgcc cgcagcaggg gtacgccgaa ttagtgctgt cattgcctgt gtactgatgc
     5161 tcattgtcac agtgtggtgt tcgctttcca tttcaaaagg gactttctac ccatatcctg
     5221 cttatcaaaa cagtagtatg gaatggagtg agagatgaat gcccgtcatt gatataaata
     5281 atgtcacaaa aatatataaa aacggtgtgg ttggcctgga ggacgtcaca ttttccattg
     5341 gccccggagt ttttgggctg ctgggccata atggagccgg aaaaactaca ttgatgcgta
     5401 tgatcgcgac gatccttcgc cccacttccg gtacaatcac cgttatgggg catgatactg
     5461 taaaggaggg ggaagccgtc cgccggaata ttggatatct gccgcaggaa ctgagcatgt
     5521 atgccaatct gaccgccctt gagttcgttt cctatatggc tcgcctcaaa ggtgtaacaa
     5581 acgctgaaga ggcgttgaac aagctggatg aagtagggat ggccgaatat agaaaccgta
     5641 aaatcgggca gctttccggc ggaatgaagc gccgcgttgg tatagcgcag gcgctggtcg
     5701 gcaacccgcg gattgtcatt gtcgatgaac cgacggcagg actcgatccg gaagaacgtg
     5761 tacgttttcg cggaatatta tcacggtttg caggaaatga ccggacagta atcctctcca
     5821 cacatatcat tgaagatgtt catcagattt gtagccatct tgccgttctg agaaaaggcc
     5881 gcctgttttt ccagggacct tccgactcgc ttcttgaccg cgtccggggt aaggtcaagg
     5941 ttgttgagct atcttccgac agagatttgg cggatctccg gaaacgtgca gtcgtcattt
     6001 ccaccactta cggcagtaaa agcttaacag tccgtattat ggatgaaaac ggccggttcc
     6061 cgcaggctcc cgtgcatgaa acacttgaag atgcctatgt ttattgtatg ggagggaacg
     6121 agcatgcgta acattgctac tattttaaaa tacgaaataa gcatgcagat aaaaagcgtc
     6181 cggctctggg ttgtatttgc atttgcactt gcagttgccc tgctggacaa tttcccgtct
     6241 gaagcaaacc tggaccgcct ggatttcctt accgaccagg gatatgtagt cagcaggata
     6301 cttgcacacg cgggtgttct gatgctgttt ggctttatgt tccttttgtc cgaccggata
     6361 aggggtgacc gaaaaaaagg gatatcggaa ctgtttatgg caagcccgtt gagcaagaag
     6421 caatatattt ggggaaaggt tcttggcaat tatgtgactg tgctttttac aatggcgatc
     6481 tattttgcaa ttaatgcact ggtccagctt gtgcacaatc ctggtccgtt cagccctgta
     6541 ccttatgcag ttggcttttt tgcaatggcc gtaccggcct gcttcttcgt ctcggcatgt
     6601 gccgtagcac ttcccgcggt gatcgatatc cgtatgttct atgtactttt ttccatatat
     6661 tttttaatga ataccgattt tatatacaca gaaaacggga tgatccggtc attatacctg
     6721 tttcaaaagg atatactgaa actggtctat acgtataaac agtttgagca aatcatacct
     6781 gccggactgt tatggaacct ggctttcctc atatgttcag gcataggagc aatcctgctt
     6841 ctgaaatgca ggaaaagcat ttggagggag acggcgtgaa ttcggagttt tcctatgagc
     6901 tgaagattgc gggaagcaac ttgtttttga tcatgctgac tgtcctgtca ttattcatcc
     6961 tggccgcggt cattggcggc gaccttgtag actgggatgt attgggattt gaggtattat
     7021 tgcctttttt tgttgcaatt atggtgtgcg aatgggtcaa gacgttatct gatcctttga
     7081 tcgacgtaat tgcagttcat gccggatcat tgtttaaatg ggttatttgc agatactttg
     7141 ccgtgttcgg cactggcagc atgctttgta tactttgcat ttcagtgttt tgtttccgga
     7201 tgcttcagcc attgaaatgc ttattgtata tatcggcacg tcttttttcc tgtcgacgct
     7261 gggcgtgctt gtttcctttt taagccgtca gccccattta ccggctgtcg tgtgcggtat
     7321 tgcctggctt ttgagcttat tacttcgggc agcttttatg gaaaacctgg tcaggcttcc
     7381 ggtaatcggc tgtttttacc cttttatacg ttttgcggct cctggcagct cattatgggt
     7441 tatgaacaaa gctgtgcttt tcttgctggg agccggtatg tgggcggggg tttattttgt
     7501 atgccgcaaa aggaaattaa tgctttgaaa gcgcagttat ccaaaaaaga aaactcacaa
     7561 ctgaaaactg aaccaaaacc ggatcttgcc caggaagtat tgaaaggccc tgcaggataa
     7621 agcgaggcgc aggaaaatcg cggataagct gctggagttt tcaagatcat ctcaatgatc
     7681 gtcttttgcc ttgcgtttaa tcgatacatg gggcatgcca tggaagccgc tggcgggcaa
     7741 ggtgttatga cgaggctttc gattcctgaa acaccgctga tgtgaggatt ttttctgtag
     7801 ccgaaaaatt cctttaaatt aggtgttata atagtttcag aaattatttg aacctttttg
     7861 gcagattcgg ggagatgcag atggttcgga ttctggtcgt cgatgatgac aaaaacacgc
     7921 ggcggctgct ggaggcggtc ctcacagcgg aaaactacac tgttttcact gcttccaacg
     7981 gcgaagaagc gcttgaggta atggacaggg agcacatcga cctggtggtc ctcgacatta
     8041 tgatgccgaa aatggacggg tacgaattta ccagggtttt gcgggaatcg aacaacaacc
     8101 tgcccatttt gatggtatcc gccaggcaat tgccggaaga caggcacaag ggctttatcg
     8161 tcgggaccga cgattatatc accaaaccca tcgacgttga agaaatgctg ctgcgcatca
     8221 aggctctcct tcgtcgcgcg cagatcgcca ccgaaaaaaa gatcgtcatc ggcgatgtgg
     8281 ttctggatta tgacagcctt acggtcacaa agaacggcga tgtccaggaa ttgccgcaga
     8341 aggagttcat gcttctgttc aagctgcttt cctatcccgg gaagattttt acccgcatac
     8401 agctaatgga cgagatttgg ggcaacgaca gcgaatccgg ctgggagacg gtgaccgtcc
     8461 acattgcgag actgcggaag cgtttcgagg gatgggaaga gttcgagatc gtgtcggtca
     8521 gaggactcgg gtataaggcg gtgaaaaagg tatgaagcct gaacagagga aacaacgcct
     8581 ggcattacat tgcttttctc aggtgtcgta ttcagctttt tcgtcgtaac gatgatcgtg
     8641 gtcggcggag tcataatcta cctgatccag cgggggatcc tggaacctgt cggccagggc
     8701 cgaagaggcc atcacctgat attcaggatg atgatagcaa gcgtaatgat cggatcgatc
     8761 ctgtcagccg ttgcgagccg catcccctta agaccggtca atgcgatcat caatgcgatg
     8821 aacaggcttg cttccggcga cttcaaaacg cgcctcagtt tcgggaagta tttgagcaag
     8881 catccgacgg caaaagagat cagccgcagt ttcaacaacc tggcagaaga gcttgaacgg
     8941 acggagatac tccgttccga ttttataaac aacttttccc acgagttcaa aactcccatc
     9001 gtttccatcg caggatttgc caaactgctg aagcacggca acctgaccga agatcaaaag
     9061 gcggagtaca tcgatataat cgaggaagag tccatgcgcc ttgccgccat ggcgacaaac
     9121 gtgctgaacc tgacaaaggt cgaaaaccag acgatcctga ccggagtgac ggagttcaac
     9181 atgtccgagc agataaggaa ctgcttcctg ctcctcgaaa acaagtggac caaaaagaac
     9241 atcacaccgg aactcgattt cggcgaacac accatccgtg ccaatgagga gatgctgaag
     9301 caggtgtgga tcaacttgat agataacgcg atcaaattcg gtgacgaagg atccacggtc
     9361 cgggtcacca ttactgaaac tgagaagaac atatttgttt ccatatccga cacaggtgtc
     9421 gagataccga tggaactgag ggaacgcatc ttccagaagt tttaccaggc ggatgagtcc
     9481 cattccggcg aaggcaacgg agtcggactt gccgttgtga aagagatcgt cagactgcat
     9541 aacggggatg ttacggtttc atcaggaaac cgcaaaacgg tgttcacggt gtcgctgccg
     9601 aaataatccc gctggctgta tgttcctgct ggtgattggg atatttttca ccgataaccg
     9661 ccgatcccgt cactgttgtc gtaaaacagg gatttccact tacggtaaat aaccgccgta
     9721 cagacatata ggggagcgga catatgcgta taatatgtcc gttcccctgt ttattttcag
     9781 tttataataa gaccgtataa tctgatcagc aataaggcag aaggaagaag tggtcatgag
     9841 gaattggttc ggcaatctga aatacaggct gcagcagttt atgtacggac gctacgggta
     9901 tgatgaacta tcccggttcg tttcggtcct tgggcttgtt ctgcttgttt tatctcttgt
     9961 accttatttg aggatcctgt attgctttgc gctgattttg cttatctgga catggtacag
    10021 ggcgttttcc agaaacgtat acaggcggca ggcggaaagg cagaaataca tggaactgaa
    10081 atacagggtc acgcagagat tttcgctctg gagaaacatg tggcgtgaca ggaagacgca
    10141 caaatattac aggtgcccgt actgcaaggc gacagtgcgt atcagaagac ccggcagggg
    10201 aaagacgata tccatcagtt gccccaaatg cgtccgggaa ttcagcaaaa aaacctgacg
    10261 tacctcatat ggaaagctgt ccggatggac ccggggaacc cggtatataa acagcttatg
    10321 tccgagtatt ccggtgaaaa ccgggcagac tacccatatg gcgaaacggt ggttgtccat
    10381 tcgccttttg gcttcataag caaactgata ttgggcatca tggctgcacg agcattgttc
    10441 tggtttcttc aaatactgtt ctacggctta ttatacagtc attgacagaa agggatctta
    10501 gtcatgcgca aatccatagg aatagacctg ggcacgacaa acagctgtgt tgcggtggtg
    10561 gaaggcggca aaccggtcat aatccccaat gaaaacggga acaggacgac gccgagcgtc
    10621 gttgccttca cgaaggacgg cgaacggctc gtcggcgacc aggcggtacg ccaggctgta
    10681 ataaacagcg agaggaccat caggtcagtc aagcgccaca tgggcaccgg ctggaaaaag
    10741 gagatcgacg ggaagccgta ttccccgcag gagatcagct caatgatact gcgcaagctg
    10801 aaggccgatg cggaagccta ccttggagag aaaataacag atgccgtaat cactgtaccg
    10861 gcatatttca acgatatcca gagacaggct acaaaggacg caggtaagat agccgggctg
    10921 aatgtgatgc gcataatcaa cgaaccgaca agtgctgcgc ttgcctatgg acttgacaat
    10981 gggaccccgc agaagatcat ggtgttcgat ctcggcggag gcacattcga tgtgtcgatc
    11041 atagagatcg gcgagggagt catcgaagtc ctggcaacca acggagacaa ccaccttggc
    11101 ggcgatgact ttgacgaacg gataatcagc catcttgccg acgtgatcca aaaagagcac
    11161 catgtcgatg tgagacgcga ttttgccgcc ctgcagcggg tgaaggaggc ggcggaacag
    11221 gcgaaaaagg agctgtcttt ttccgagacc agctatatcg ctc
//
LOCUS       DAOSCC010000060        11231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3861_length_11231_cov_4.347530, whole genome shotgun sequence.
ACCESSION   DAOSCC010000060 DAOSCC010000000
VERSION     DAOSCC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3861_length_11231_cov_4.347530"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLG72_04775"
     CDS             complement(<1..418)
                     /locus_tag="PLG72_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOJ80145.1"
                     /translation="MMKHYLEEVSDVLGSLGTDRNGISPEKAALLLERHGRNKLAESR
                     KKTVFERFADQLKDPMVIILIAAAIISGVVGETADAAVILAVVILNSILGVVQEGKAE
                     KAIEALQKLSSPYSRVRRNGHVMHIKSEELVPGDIVL"
     gene            complement(554..1420)
                     /locus_tag="PLG72_04780"
     CDS             complement(554..1420)
                     /locus_tag="PLG72_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80146.1"
                     /translation="MKPVRQEFTSRQHMISRDFEFYHYKDEHPVEVEYHNHLFYEVFS
                     FISGDVTYIVEGKSYRLKPGDIILINNREIHKTFVKPGKVYERCVLWIDPDFIRTLGG
                     DTDLTMCFERSAMRNYNLLRPRTEYYIMIKNAMTCLQNAVFSEGYGNGALKKAYLTEL
                     LVYLNRAFLDSQDEDIELDVVYDEKVSSVISYINENLQEDLSLESLAGRFYTSKYHLL
                     REFKKHTGYTIYQYIRKKRLITARVLLKDGLMVTEVCQKCGFGDYSNFIRSFRKEFGH
                     PPGHYKNYGSTN"
     assembly_gap    1606..1615
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1616..4059)
                     /locus_tag="PLG72_04785"
     CDS             complement(<1616..4059)
                     /locus_tag="PLG72_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ80147.1"
                     /translation="MKIAFSTLGCPDFDWPDIYSMAKDLGFDGIEIRGLGKDIFAVRA
                     KPFTENELPNTIKKLKELRIEIPCLSSGCCLKFADKAEQNHNEIVQYIELANKLGTPY
                     VRILADLEPQPEGEVDDGAVLAALRRLIPIAEEKGVTLLIETNGVYADTKRLSELLAS
                     AASDNVGALWDVHHPYRYAGESADTTVKNLGAYIKYVHVKDSVMQDGKVSYRMMGEGD
                     LPMDDFMMALHSVNYEGYISLEWVKRWASDLSDAGVVFPHFVNYMNSYIEKDTSKGRL
                     YYNNAKTGQYIWEKDVLIDLTFPQVLDRVVAEFPDQYAFRYTTHDYTRTYKEFRDDVD
                     TFARSLIALGVKPGDHVAIWATNVPQWFITFWATVKIGAVLVTVNTAYKIYETEYLLR
                     QSDTHTLVMIDGYKDSNYVEIIKELCPELETSEPGKPLHIKRLPFLRNIITIESRVKG
                     CLTWEEALALAEKVPVEEVFRRAASIHKHDVCNMQYTSGTTGFPKGVMLTHYNVVNNG
                     KCIGDCMDFSTADRLLIQVPMFHCFGMVLAMTAAMTHGTTMCPIPYFSPRVSLDAINR
                     EKITAVHGVPTMFIAMMEHEDFAKTDFSHMRTGIMAGSPCPIKVMQDVVDKMNMKEIT
                     IVYGQTEASPGCTQSRVDDPIEIRVNTVGRALPGIECKIVDPATNKDLPPNTDGEFVV
                     RGYNVMKGYYKMPEATAAVIDEDGWLHTGDMARMDEHGYFKITGRIKDMIIRGGENIY
                     PKEIEDFIYTHPKVSDVQVIGVPDKQYGEEIMACVILKEGVTMTAEELQEYCRTHMAK
                     HKTPRYVEFVDSFPMNA"
     gene            4640..6202
                     /locus_tag="PLG72_04790"
     CDS             4640..6202
                     /locus_tag="PLG72_04790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ80148.1"
                     /translation="MQRKPVLLIAFYNKKALGVRYLERSLARAGHRPVVLFLKDFSSR
                     MPRPVNDRELMLLRNLVSELDPGLIGLSVMSSLYLEQVMAVSRFLRHEFRIPQVWGGV
                     YASMFPEKCLVYADFVLRGECEEAIVELADAVLGNRDFHHIKNLAYKTPSGGNGATAV
                     INELRPLCGDLDRLGYPVFSNENKYFIDNGRIISGDPALDSVSYELSASRGCPFVCSF
                     CSSANIKRMYAGKGRFVRFRSPTNIIAELGEALENIKRLKVIHFWDEIFPDDREWIDD
                     FATQYKSHIGLPFVIWVHPLKTEEYTISKLVDAGLYKAVMGIQSGSQRIRNDIFHRRE
                     TREDILKASGILAGCGVRQITYDFILRHPFENEEDIRQTYELCTELERPFELQLHGLG
                     FLPGTDITGIAEKYGIDAPLSESGPGIPMKTVYRDYWGRKSSDPAINFWYSLIYMSQF
                     GPGLRMAKYLSKARKARIVMKMAMVLSTLLRPAAGARSLYKKARMLAVAYIRKFSAAR
                     AERQAVGCHEGK"
     gene            6224..7084
                     /locus_tag="PLG72_04795"
     CDS             6224..7084
                     /locus_tag="PLG72_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80149.1"
                     /translation="MKTRFVRISFIILAAAAGLVILLILSMAAVEPMAHIYPDYPMVD
                     LEPVLQKDTFNEEDYRLLYLQTGLGKPAVDEIMERYSDWRERLLRFQRGFFSSIDFIC
                     EKNSPVSREEAIIDENGNYAHGTDLAPVHDGYILVTKSSHTYGWRNGHAALVVDARNG
                     KTLESVVLGQNSAIQGIEKWTVYPNFMLLRPKGATYEEMQEVAQTALELLHDIPYDLT
                     VGIFSPKSAARDKIKGTHCSHLIWQAYMYHSYDLDSDGGMLVTPKDIANSPKLEIVQV
                     YGVDPREIWP"
     gene            7262..8182
                     /gene="trxB"
                     /locus_tag="PLG72_04800"
     CDS             7262..8182
                     /gene="trxB"
                     /locus_tag="PLG72_04800"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOJ80150.1"
                     /translation="MHDVIIIGGGPAGYTAALYSARAGLDTLVLEKLAPGGQMATTLS
                     MENYPGFEEPISGPDLALRMENQARKFGAVVEYDTVTEVIPDNGLKTVKTLKNTYRCK
                     AVILCMGAAPRKLGIPKEDLLRGSGVSYCAVCDGALFKGRPVAVVGGGDTAAENALYL
                     SRFCPKVWLIHRRDRLRASKVLQKELFSNKNIEFIWNAVVEELRGQSSLEGITVRDLK
                     TGITRVLDAEGLFISIGVTPSTSLVQGKVAIDDGGYIITDEGMRTSVPGVFAAGDIRS
                     KVLRQVITAASDGAVAAHSAEQYINDTFSK"
     gene            complement(8271..8699)
                     /gene="mutT"
                     /locus_tag="PLG72_04805"
     CDS             complement(8271..8699)
                     /gene="mutT"
                     /locus_tag="PLG72_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HOJ80151.1"
                     /translation="MKDVTAAIILNSENKVFIARRAPGENMAGGWEFPGGKLEQYETA
                     EECLKRELFEEFGIEVAVKDFFCESIYEYPQGTIRLLAYFAEVTGGEMHLSVHDDYRW
                     VDADELSGYELLPADIPVAEKLAGMMAGRNNHGCAAPGPG"
     gene            9003..10199
                     /gene="ytvI"
                     /locus_tag="PLG72_04810"
     CDS             9003..10199
                     /gene="ytvI"
                     /locus_tag="PLG72_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOJ80152.1"
                     /translation="MNAKYRESLKRLGIFVLVVISVVGGVFLAVRLAFFLLPFLIAFA
                     LSSLMEPLIRVLDRKLHIKRKISAPIILLLLMMGIIITLVVLGILRLVEEIRGLIAAA
                     PAFFSNMYAEIVELIKKSAEHIDWVPVEITDNLGTIIANLSNTVTNFGKTLVKGAYTT
                     ALSLPEVIIFTIVTILATYFMAKDRHVIAAVLRRHLPETWVDRILSIKNDLFMAIFGY
                     LRAALIIMVITFTELLIGLTIIGVKYTLLLAFLIAVIDALPILGTGSVLIPWSLYSFI
                     TGDIRMGVSVIVLYLVILVVRQFVEPKVVGQQIGVHPLLTLLAMYTGLKLIGFIGMIL
                     GPIAFILIRNILVTIYRNKPIKDIIHFEFTESDADTDGSGRDMHISGTADPTPGVHGD
                     PDGHDN"
     gene            10367..10882
                     /locus_tag="PLG72_04815"
     CDS             10367..10882
                     /locus_tag="PLG72_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOJ80153.1"
                     /translation="MTTISLTTKERMLLEDQKSHEEICIEKYSKYAQLASDPQLKNIF
                     INNGKIEQEHLDTLNQLLKGQVPSMGQQGGSQQSGTGMQNNAGGQQMQQNAQSSQQAS
                     QASGVVSDESLCNDMLMTEKYVSGTYDTAIFEFKDTQVRDILNHIQKEEQKHGEAIFK
                     YMQSKGMYKPK"
     gene            complement(11111..>11231)
                     /locus_tag="PLG72_04820"
     CDS             complement(11111..>11231)
                     /locus_tag="PLG72_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOJ80154.1"
                     /translation="TVGDPFKDTAGPSINTQITVVSLVSSLTAGLFATISLFG"
BASE COUNT         2711 a         2925 c         2841 g         2744 t
ORIGIN      
        1 gcaggactat gtcaccgggc accagttcct cggattttat atgcatcaca tggccgttcc
       61 ttcttaccct ggaatacggc gatgataatt tctgcaacgc ctcgatggcc ttttcggctt
      121 ttccctcctg aacgaccccg agaatggaat tgaggatcac taccgcaagg ataacagccg
      181 catccgctgt ttcaccgacg acgcccgaga ttatcgcggc agctatcagg ataatgacca
      241 tcggatcctt cagctgatct gcgaatctct caaagacagt ctttttgcgg ctttcagcca
      301 gcttgttcct gccgtgcctt tcaagcagca gcgccgcctt ttcggggctt atcccgtttc
      361 tgtcggtccc aagggacccc agtacatccg acacttcctc caaataatgc ttcatcatta
      421 cttccctcca atattggttt tcggtgatcc gggcatttga tggtattaaa taccatcaaa
      481 gcaattatac cggttatgac ttataattcg tatacaatac gaaaagaaaa cttgcaatgt
      541 ttcacaaaac atgtcaattc gtactgccat agtttttata gtgccccgga ggatgtccga
      601 attccttcct gaaggagcgg atgaagttgg aataatctcc aaagccgcac ttctggcaga
      661 cctcggtgac catgagaccg tccttcagaa gcaccctggc tgttatcagg cgtttcttgc
      721 ggatgtactg gtagatggta tagccggtgt gctttttgaa ttccctcaaa aggtgatact
      781 tactggtgta gaacctgccg gccagcgatt cgagggacag gtcctcctgc aggttttcgt
      841 tgatatagct gatcacgctg ctgactttct cgtcgtacac gacgtccagt tctatatctt
      901 cgtcctggct gtcgaggaaa gcgcggttca ggtaaaccag gagttccgtg agatatgcct
      961 tcttcagtgc gccgttcccg tacccttcgc tgaaaactgc attctgcagg catgtcatcg
     1021 cgttttttat catgatgtaa tattcggtcc gcggccgcag caggttataa ttcctcatgg
     1081 cggaacgctc gaagcacatg gtaaggtcag tgtcccctcc cagggtcctg atgaagtcgg
     1141 ggtcgatcca cagaacacag cgttcgtata cctttcccgg cttcacgaac gttttgtgga
     1201 tctccctgtt atttatcagg atgatatcgc cgggtttcag cctgtaggat ttgccctcga
     1261 ctatatacgt cacgtcgccc gaaataaagg aaaacacctc atagaagagg tggttgtggt
     1321 attccacctc gaccgggtgt tcatccttgt agtggtaaaa ctcgaaatcc ctgcttatca
     1381 tgtgctgccg gctggtaaat tcctgcctga ctggcttcat agcatcatcc tcgatccgat
     1441 gatacttaat tatatactaa tatactaaag caatttttgc atgcaatcaa ccgatcagca
     1501 ttttttattg catttacatg catcaggcag tctctatctt gcttgcctcc tgcaggctga
     1561 gtttctctat ggcccattcg cgcatcttgt acttgaggat ctttcnnnnn nnnnngcgtt
     1621 catcgggaac gagtcgacga attccacata gcgcggggtc ttgtgtttcg ccatgtgtgt
     1681 gcggcagtac tcctgcagtt cctcggcggt catcgtgacc ccttccttca gtatgacaca
     1741 ggccattatc tcttcaccgt actgcttgtc aggcactcct atgacctgta cgtcgctgac
     1801 cttcgggtgg gtatagataa agtcctcgat ctccttcgga taaatgtttt caccgcccct
     1861 gatgatcata tccttgatgc ggccggtgat cttgaaatat ccgtgctcat ccatcctcgc
     1921 catgtcgccc gtatgcagcc agccgtcctc gtcgataact gctgcggtcg cctcgggcat
     1981 cttgtagtat cccttcatga cgttatagcc gcggacaacg aattcaccgt cggtattagg
     2041 aggcagatcc ttattggtcg cgggatcgac tattttgcac tcgatccctg gcagtgccct
     2101 gcccacggtg ttgacgcgga tctcgatggg atcgtccacg cggctctgtg tgcagcccgg
     2161 tgatgcctcg gtctgtccgt agacgatcgt tatctccttc atgttcattt tatccacaac
     2221 gtcctgcatc actttgatag gacacgggct ccccgccatt attcccgtcc tcatgtgtga
     2281 gaagtccgtt tttgcgaaat cctcatgctc catcatggcg atgaacattg tcggaacgcc
     2341 gtgcacggcc gttatctttt ccctgtttat ggcatcgagc gacaccctcg gagagaagta
     2401 cgggatcgga cacatcgtcg tgccgtgcgt catggcggca gtcatcgcca gcaccatgcc
     2461 gaagcagtgg aacattggca cctgtatcag cagcctgtca gccgtggaga aatccatgca
     2521 gtcccctatg catttgccgt tattcacaac gttatagtgc gtgagcatga cgcctttcgg
     2581 aaatcccgtc gttccggacg tgtactgcat gttgcataca tcgtgtttgt gtatggaagc
     2641 ggcacggcgg aacacttcct caacaggcac cttctccgca agcgccagcg cctcttccca
     2701 tgtaagacag cctttcactc tcgattctat cgttatgata ttgcgcagga acggcagcct
     2761 ctttatatgg agcggctttc cgggctccga ggtctcgagt tcgggacaca gttccttaat
     2821 gatctccacg taattcgaat ccttgtaacc gtctatcatg acaagtgtat gggtgtcgga
     2881 ctggcgcagc agatactcag tttcgtatat tttgtacgca gtattgaccg taacgagcac
     2941 cgccccgatc ttgaccgtcg cccagaatgt aatgaaccac tggggcacat tggtcgccca
     3001 gatggccaca tggtcgcctg gcttgactcc aagggcgatg agggatcttg caaatgtatc
     3061 cacatcatcc ctgaactctt tatatgtcct ggtatagtca tgtgtcgtat aacggaaagc
     3121 atactggtca gggaactctg ccacgacacg gtcgagcacc tgcgggaatg tcaggtcgat
     3181 gagcacgtcc ttttcccata tatactggcc ggtcttggcg ttattgtagt acaggcgtcc
     3241 ttttgacgtg tctttttcga tataggagtt catatagttc acaaaatgcg ggaacacgac
     3301 gccggcgtca ctgagatcgc ttgcccacct tttgacccat tccagggata tatatccttc
     3361 gtagttcaca gaatgcaatg ccatcatgaa atcatccatc gggaggtccc cttcgcccat
     3421 catcctgtag gagacctttc cgtcctgcat gacggagtcc ttgacatgca cgtattttat
     3481 atatgcgcca aggttcttca ccgtggtgtc tgccgattcg cctgcgtaac ggtaaggatg
     3541 atgcacgtcc cagagcgcgc cgacgttatc gcttgcggca cttgccaaaa gctcgctcag
     3601 gcgcttcgta tctgcatata cgccgttagt ctcgatcagc agggtaactc ccttttcctc
     3661 ggcgatgggt ataaggcgcc tgagtgccgc gagaactgct ccatcgtcca cttcgccttc
     3721 cggctggggc tcaagatccg ccagtatacg cacatagggc gtgccaagtt tattcgcaag
     3781 ttcgatatac tgcactatct cattgtggtt ctgttctgcc ttatccgcaa acttcaggca
     3841 gcagcccgaa gaaaggcacg ggatctcaat acgcagttcc ttcagctttt tgatggtgtt
     3901 tggaagttca ttctcggtaa atggctttgc cctgaccgca aatatgtcct ttccgaggcc
     3961 cctgatctcg atgccgtcaa atccaaggtc cttggccatt gaataaatgt cgggccagtc
     4021 aaaatcggga catcccagtg ttgaaaatgc aatcttcatc tcaatctcct cccagatcaa
     4081 ataaaatcat tgcaaatgag cattttgcca ggttcggccg gcatcagccg gcaaaataaa
     4141 aagcttccgt ctccgacatt cctgccagag acgaaagctc agcttccgcg gtaccactct
     4201 gattggtatg gatataccca ctctaaggca tcgatcgttc aacgagtaat gccctcccgt
     4261 tgtaacggcg ggaagcccgg tcgcacctac ttgccacggg acggcgtttc ctgcttcgcc
     4321 atgtttgtct gccccgctta cggctttcag ctgactgctc ggaggtgagt ccacgatccg
     4381 ccccgtgtgc tgtttcacac cggccaacag ctctcttgaa cacagccgga cggcatgttt
     4441 tccccgtcat tgcatttgat ctttttttgt atgctaccag aaacccgttg ttttgtcaat
     4501 atgtatcaag gctgtcgggt gtaccaggct gccgacaggt ttcgcatatt tgctgaaaac
     4561 cggtatttat ttctccatac gggacattat aaggaaatgg atgaaatgat cggcaacata
     4621 ttgtatcgga ggaagcggca tgcagcgaaa acccgtactt cttatagcgt tctacaacaa
     4681 aaaggctctc ggggtaaggt acctggaaag gtcgctggcc agggcaggcc acaggccggt
     4741 cgtactgttc ctgaaggatt tcagcagcag gatgccccga cctgtgaacg acagggaact
     4801 tatgctgctc aggaacctgg tttcggaact ggatccgggt ctgatcgggc tgagcgtcat
     4861 gtcatcgctg taccttgaac aggtcatggc agtcagcagg ttcctccgcc atgaattccg
     4921 catcccgcag gtatggggcg gagtatatgc atcgatgttc ccggaaaaat gcctggtata
     4981 cgcagacttc gtattgcgcg gcgaatgcga ggaagccatc gtcgagcttg ctgacgccgt
     5041 gcttgggaac cgggactttc atcatataaa gaatcttgcg tacaaaaccc cttccggcgg
     5101 aaacggcgcg acagcagtga tcaacgaact tcgtccgctg tgcggagacc tggacaggct
     5161 tggatatccc gtgttcagca acgaaaacaa atactttatc gacaatggcc gtatcatatc
     5221 cggcgatcct gcgctggact cggtcagcta tgaactgtcc gcttcaaggg gatgtccgtt
     5281 cgtatgttca ttctgctcat ctgcaaacat taagaggatg tatgcgggca aaggcaggtt
     5341 cgtgcggttc aggagcccga cgaacattat agccgaactc ggggaagcgc tggaaaatat
     5401 aaaacggctg aaagtcattc atttctggga tgagatattc cccgacgaca gggaatggat
     5461 cgatgatttc gccacacagt acaaatcgca tataggcctg ccatttgtga tatgggtaca
     5521 cccgctgaaa acagaggaat acaccatatc gaaactcgtg gatgcaggcc tgtataaagc
     5581 ggtcatggga atacagagcg gatcgcagcg catcaggaac gatatattcc accgccgcga
     5641 gaccagggaa gacatactga aggcatcggg aatacttgcc ggatgcgggg tacgacagat
     5701 cacatacgat ttcatacttc gccacccttt tgagaatgaa gaggatataa ggcagacata
     5761 cgaactgtgt accgaactgg aacggccgtt cgaactgcag cttcacggcc tgggcttcct
     5821 gccggggacc gatataaccg ggatagcgga aaagtacgga atagacgcac cattatcgga
     5881 atccggtccc ggcatcccga tgaaaacggt gtacagggat tactggggcc gaaagagcag
     5941 cgatcctgca ataaatttct ggtattcgct catatatatg agccagttcg gtcctggcct
     6001 ccgtatggcc aaatacctga gcaaagcccg taaggcacgc attgtcatga aaatggcgat
     6061 ggtactgtca actctgcttc gccccgccgc aggcgcaagg agcctgtata aaaaggcccg
     6121 gatgcttgca gttgcataca tcaggaagtt cagcgccgcc agggcggaaa ggcaggctgt
     6181 tggatgccac gagggcaaat gatgtccggc ggtggagcga tccatgaaaa ctcgtttcgt
     6241 ccgtatctca ttcataatac ttgcggcggc cgctggcctg gtgatcctgc tgatactgtc
     6301 catggctgct gtggagccga tggcgcatat atatcctgat tacccgatgg tcgaccttga
     6361 gcctgtcctg caaaaagaca cgttcaatga agaggattac aggctgctgt atctgcagac
     6421 aggcctgggt aaacctgccg tggatgagat catggaacgg tacagcgact ggagggagcg
     6481 gctgctgcgc ttccagcgcg gcttcttcag cagcatcgat ttcatttgtg aaaaaaacag
     6541 ccctgtatca agggaagaag ccatcatcga tgaaaacggc aattatgcgc acggaacgga
     6601 tcttgctcct gtccacgacg gttacatact tgtgacaaaa tcatcacata cttacggctg
     6661 gcggaacggg catgcggcgc tggtggtaga cgcacggaac ggaaaaacac tggagtcggt
     6721 ggttctgggg cagaactctg ccatacaggg catagagaaa tggacggtct atcccaactt
     6781 tatgcttcta aggccaaaag gcgccacata cgaggaaatg caggaggtcg cacagacagc
     6841 gctggaactt ctccacgaca taccctatga tctgacggtc ggcatattca gccccaagtc
     6901 cgcggccagg gataagataa aagggaccca ctgctcccat ctgatctggc aggcatacat
     6961 gtaccacagt tatgacctcg acagcgacgg agggatgctt gtcactccga aggacattgc
     7021 caacagtccg aaacttgaga tcgtacaggt ctatggagtc gacccgcgcg aaatatggcc
     7081 gtaacgccca ggcctgacca tggtggcatg tctgtccgcc ggtccctgct gctatattgt
     7141 tactttacat aacaactgtg gtatagtaat tattgataat cgcttgaatc gtgccaggaa
     7201 aagcgcttgg gcacatatta tacaacaata atgtaaagcc aggtgtataa aaatgcaaga
     7261 catgcacgac gtgatcataa taggcggcgg tccggcaggc tatacggcag cgctctacag
     7321 cgcccgggcg ggtcttgaca cgctcgtact cgagaagctg gctcccggag gccagatggc
     7381 aacgacgctg tcgatggaaa actacccggg gttcgaggaa ccgatcagcg ggccggacct
     7441 ggcattgagg atggaaaacc aggcaaggaa attcggcgct gtcgtggaat atgacacggt
     7501 tacggaagtc atcccggaca acggtttaaa gactgtaaaa acccttaaga acacttaccg
     7561 gtgtaaggcc gtgatattat gcatgggcgc cgcgccaagg aaacttggta tcccgaagga
     7621 agacttgctg aggggttcgg gcgtttcata ctgtgcagta tgcgacggcg cacttttcaa
     7681 agggaggccg gtcgcagtcg tcggaggcgg cgataccgca gcagaaaatg cgctctacct
     7741 ttccaggttc tgcccgaagg tatggctcat acacaggagg gacaggctgc gcgcctcaaa
     7801 ggtactgcag aaagaactgt tcagcaacaa aaatatagag ttcatatgga acgccgtggt
     7861 cgaagaactc cgcgggcaat ccagcctcga aggcataacg gtaagggatc tgaaaaccgg
     7921 catcacaagg gtgcttgacg cggaaggcct gttcatatcg ataggggtga ctcccagtac
     7981 ttctcttgtc caggggaaag tggccataga tgacggcggt tatatcatca ctgatgaagg
     8041 catgcggaca agtgtgcctg gtgtgtttgc tgcaggcgat atccggagca aagtcctcag
     8101 gcaggtcatc accgcagcat ccgacggcgc cgtggcggcc cattcggcag agcagtacat
     8161 caatgatacc ttctccaaat aaagatcatc ggaatggcag aagcctgcat catgccgtcg
     8221 atcgcatgtc aatgcaggct tttgcgcgtc tttgctcacg tcccggtacc tcaacctggt
     8281 cccggggctg cacagccatg gttatttctt cccgccatca tcccggcaag tttctcagcc
     8341 acaggtatat cggcgggaag cagctcgtat ccggacagtt catcagcatc gacccacctg
     8401 taatcgtcat ggacactgag atgcatttcc ccgcccgtta cttcggcgaa atacgcgagc
     8461 aggcgtatcg taccctgcgg atactcatat atgctttcac agaaaaagtc ctttacagca
     8521 acttctatgc caaattcctc gaagagttcc cttttcaggc attcctcggc agtctcgtac
     8581 tgttcgagct ttcccccggg gaactcccat ccgcccgcca tgttttcacc aggcgctctc
     8641 ctggcgataa agaccttatt ttcgctgttc agaattatcg cagcggtgac atccttcatt
     8701 ttccgcatat ctgtctcctg aatatatcta ttgagttgcc ccctggtttg caaatctgat
     8761 tttttgctga aattgcaacg attggtcgaa aaatactata aatagtatat agcatattca
     8821 agtatctgtc actcaggctt tgtctattct cctgcctgca ggatgccgtg gcgggaacgg
     8881 gcatttactg tcggttcgac agtcttacgg ctgcaggaca tagatccggc catcttatta
     8941 aaagaaagca gattgacgaa tatacgcgca taatttaata tataactgta caggagtcgc
     9001 ctatgaacgc aaagtacagg gaaagcctga aaagacttgg catattcgtt ctggtcgtca
     9061 tatctgtagt gggaggagtc ttcctcgctg tcaggctggc gttttttctc ctgcctttcc
     9121 ttattgcttt cgccctgtca tcactgatgg agccactgat cagggtactt gacaggaaac
     9181 tgcatatcaa aaggaaaata tcggctccga ttatactcct gctgctgatg atggggataa
     9241 taataaccct ggtcgtactc gggatcctga ggctggtcga ggagataaga gggcttatcg
     9301 ctgctgctcc cgcatttttc tcaaacatgt acgcagagat cgtcgagttg atcaaaaaga
     9361 gtgccgagca tatcgactgg gtacctgtgg agatcacgga caacctggga accatcatag
     9421 ccaatctttc caatactgtc accaattttg gcaagacact cgtgaaaggc gcctatacga
     9481 ccgcattgtc gcttcctgaa gtgatcatat ttaccatagt gactatactt gcgacttatt
     9541 tcatggcaaa agaccgtcat gtgattgctg ccgtactcag gcgccatttg cccgaaacct
     9601 gggtggaccg catcctgtcc atcaagaatg acctgttcat ggcgatattc ggctatctga
     9661 gagcagcgct catcataatg gtgataactt ttacagaact cctcatcggc cttaccatca
     9721 tcggcgtcaa atacacgctg cttcttgcat tcctgatcgc tgtcatagac gcgcttccga
     9781 tccttggcac cggaagcgtc cttataccgt ggtcgctgta ttccttcatc accggcgata
     9841 tcaggatggg tgtttcagtc atcgtgcttt acctggtcat actggtcgtc cgccagttcg
     9901 tggaaccgaa ggtggtgggc cagcagatcg gcgtacatcc gctgctcacg cttcttgcga
     9961 tgtacacagg ccttaagctg atagggttca taggtatgat actaggtccg atagccttta
    10021 tcctcatccg caacatcctc gtgacgattt acaggaacaa gcccatcaag gacataatac
    10081 atttcgaatt taccgagtcg gacgccgata ccgacggctc cggccgcgat atgcatatca
    10141 gcgggactgc tgatccgact ccgggtgtgc atggcgatcc ggacggacat gacaattgag
    10201 acataaaagc catgcagtgc gaagctgtca ccggtccagc cggaacaggc acaggtgagc
    10261 ctgcagccga aagaggcgtc acaagcgaag atccttaagc gattcgcaga tcgatgcgca
    10321 tatattttac cctttggaaa aaactattga cggaggtgta aaatatatga cgactatcag
    10381 tttaacgacg aaggaaagga tgcttctcga ggatcagaaa agccacgagg agatatgcat
    10441 cgaaaagtac agcaaatatg cacaacttgc ttcagatccg cagctcaaga acatcttcat
    10501 caataatgga aaaatcgaac aggaacatct ggatacgctg aaccaactgc tcaaaggaca
    10561 ggtaccttcg atgggacagc agggcggcag ccagcagagc ggtacgggca tgcagaacaa
    10621 tgccggcggg cagcagatgc agcaaaacgc ccaatcgtcc cagcaggcca gccaggcatc
    10681 cggcgtggta tcggacgaga gcctttgcaa cgatatgctt atgaccgaga aatatgtatc
    10741 cggcacatat gacaccgcca ttttcgaatt taaggacaca caggtacggg atatcctcaa
    10801 tcatatccag aaggaggaac agaagcacgg ggaagcgatt ttcaaataca tgcaaagcaa
    10861 gggcatgtat aagccgaaat gaaggtctca tgggcatagc gcccctcagt ggagtatcga
    10921 actgagtgaa gaactgatct atatggacat atgtaaaggc ctgtcctgat aacatgagcc
    10981 ggttcgctcc ctccgctttg ctgcaggaaa aaacatcccc tccgctgagg aaggggatgt
    11041 ttttatatcg gttatcaagc aactatatac gatcgcggat atgtccggtc cgaaaacaga
    11101 ccgccggtca tcagccaaac agtgagatcg ttgcgaacaa acctgccgta agggacgata
    11161 cgagcgaaac aactgtgatc tgcgtattta tggacggacc tgcggtatcc ttgaaaggat
    11221 cgcctaccgt g
//
LOCUS       DAOSCC010000061        11210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3868_length_11210_cov_3.996414, whole genome shotgun sequence.
ACCESSION   DAOSCC010000061 DAOSCC010000000
VERSION     DAOSCC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_11210_cov_3.996414"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLG72_04825"
     CDS             complement(<1..288)
                     /locus_tag="PLG72_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOJ80155.1"
                     /translation="MHKKLFIPGPVEVRDDVLQKMATPMIGHRTKEASALQRSITEKL
                     QKLMYTKNMILLSTSSGSGLMEGAIRSCTVRRAAVFSVGAFGDRWYKMAVSN"
     regulatory      complement(367..452)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            876..3305
                     /locus_tag="PLG72_04830"
     CDS             876..3305
                     /locus_tag="PLG72_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOJ80156.1"
                     /translation="MPFKLTRPNNQDEPAKAGMQDKPTITVSVRELLEFAMQSGDLRT
                     ASYSPSRAAEGTRGHAEIRAIIKQMLSDDAIYETEVPITFRLEGDNLVLEITGRIDGL
                     LKDAAGDTVHEIKTTMLPLEQIEHDHDPQHWAQVKCYAYMYARMHGLDRIAIMLTYYQ
                     LDEKQEKSFTDIYDIDSLESFFMPLAGDYMAWQEKMNNWAIVRDRSIHQLDFPYPERR
                     KGQQELMDDVYDTIGVRGLLFAQAPTGTGKTVAVLFPSVKALGDRLVSKIFYLTAKTT
                     TRSLAEKSLEDMRKKGLRIKSITITAKEKVCFNGTHFCNPEICMYKEDYYGKIKKALE
                     DALQEDALDRTVIEKYASKYSICPFELTLDLSLLCDVIICDYNYLYDPRVKLSRYFSK
                     RGNYCFLVDEAHNLVDRARDMYSAEISRRSYFRLKKQAKEELPEIYDALDRMHKCFLD
                     ISKALAEDSGDQKKGFYLVERAYPDELCRPIERFTGLVGDLLPRILAVSFMDQLLEVY
                     FDSLHFLRISELYDERYVTCYDKSATDFKIKLFCVDPSKLLRDAMNKGRASILFSATL
                     SPIGYFTSMLGGGDDARVTVIPSPFPRENLCVYIDDSISTKYKTRMFSYDRIAETILE
                     TVSARTGNYMAFFPSFEYLNEVYYRFMGISRGIRSLCQTPGMSEAARQEFLDEFEKTG
                     ETSLVGFAVMGGVFSEGIDLAGDRLSGAIIVGVGLPQICNERNIIRAHFDERSGNGFE
                     YAYIYPGINRVLQAAGRVIRTEDDMGVVILLDERFSHYNYRELLPPEWTPIQKMSDGI
                     LLTDVLQDFWG"
     gene            complement(3389..4636)
                     /gene="lysA"
                     /locus_tag="PLG72_04835"
     CDS             complement(3389..4636)
                     /gene="lysA"
                     /locus_tag="PLG72_04835"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOJ80157.1"
                     /translation="MKVYFTEQTNFYGSSKPSELIREYGSPLYVYNESILRQRCRELA
                     GLVSYSNYRSNYSIKANSNLELLKIIRDEGLHADAMSPGEIHVLLAAGYRPDEILFVA
                     NNVSAEEMQYAIDKGVLVSCDSLSQLRQYGRLNPGGKVAVRFNPGVGAGHHAKVVTGG
                     ENTKFGINLEMMDEVKAILKEYRLSLAGINQHIGSLFMEPSSYLQAARALLNIASNFE
                     DLEFIDMGGGFGIPYRKQEGQQRLDLEEMGRGLSQLMEDWTANYGKRVRLKIEPGRYV
                     VAECGVLLGTVHAVKQNGSKTYIGTDVGFNVLIRPAMYDSHHDIEFYRNDAVLKTDTV
                     RKATVVGNICESGDIIANDRELPEAAEGDIIGVMDAGAYGYSMSSNYNNRLRPAEVLI
                     GLDGSPRLIRRRDTFEDLMRGFI"
     gene            complement(4757..4882)
                     /locus_tag="PLG72_04840"
     CDS             complement(4757..4882)
                     /locus_tag="PLG72_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80158.1"
                     /translation="MAGKKKKSVAGMLFYTFSTILLLTLLIICIINFLRDGRPFG"
     gene            complement(4970..5566)
                     /gene="yqeK"
                     /locus_tag="PLG72_04845"
     CDS             complement(4970..5566)
                     /gene="yqeK"
                     /locus_tag="PLG72_04845"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOJ80159.1"
                     /translation="MLNLDEMMKKMQHMLTPHRFRHSINTMETCIRLAEKYGADVDKA
                     AVAGLIHDCAKDLDKDAVLPICEKYGIIVDEISRSQPKLLHGEIGAHMARELFGVDDP
                     QILAAVADHTLGRPGMDDISSIVFIADYIEPGRDFEGVDEIRKAAEESLEKAIVAGIN
                     STIRHVLKKGQPLHPRSVLTRNWALQKLKEREQQDQEH"
     gene            complement(5649..6479)
                     /gene="nadD"
                     /locus_tag="PLG72_04850"
     CDS             complement(5649..6479)
                     /gene="nadD"
                     /locus_tag="PLG72_04850"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOJ80160.1"
                     /translation="MATKGLSQQFVPLYDKCCNHGCTAVAYSLIDRKEMVMENRNGTG
                     KTEYMSGTQYKTGARYSTGTQCKPGAPDRKPQRIGIMGGTFNPIHNGHLIIAEDVREK
                     AGLDKVLFIPSGQPPHKPGCEVADAEHRFEMVRLAVSSNPYFEASRIEIDRNGLTYTI
                     NTLQELREDYGEETAIYFIVGADVVHELVTWREYEKVFRLCEFIAVLRPGYDEAGFNE
                     AIHRISTGKGARIHPVPSHLVDISSTDIRERCRKGRSIKYLVPGSVERYIYEKGLYRG
                     "
     gene            complement(6510..7772)
                     /gene="purD"
                     /locus_tag="PLG72_04855"
     CDS             complement(6510..7772)
                     /gene="purD"
                     /locus_tag="PLG72_04855"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ80161.1"
                     /translation="MKVLIVGGGGREHAIAWKISQSPKVSKLYCAPGNGGIASLAQCV
                     PIKAMDIEGITSFAKSEGIDLVVVAPDDPLAAGMVDALENAGIRAFGPVKAAAEIESS
                     KVFSKNLMKKYGIPTAAYEVFDNADNALEYLRTCSYPTVVKADGLALGKGVIIAGNYD
                     EAEKAVRSIMSDRIFGSAGNRVVIEEFLVGTEVSILAFTDGETLVPMVSSQDHKRAMD
                     NDMGPNTGGMGTFSPSRLYTAEINDYCMKNIFMPTIEAMRKEGRRFKGVLYFGLILTA
                     DGPKVLEYNARFGDPETQVVLPHLKTDIIDIFEAIIDERLSEINIEWENRSAVCVILA
                     SGGYPGKYETGLEITGIDEAEKDPSVIVFHAGTKLEAGKYYTAGGRVLGVTAVADTID
                     DARAKAYAAVGKIKFEGMHYRRDIGIKK"
     gene            complement(7914..9461)
                     /gene="purH"
                     /locus_tag="PLG72_04860"
     CDS             complement(7914..9461)
                     /gene="purH"
                     /locus_tag="PLG72_04860"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOJ80162.1"
                     /translation="MIKRALISVSDKTGVVELAQALAGRGVEIISTGGTYKTLADAGI
                     KVIGISEITGFPECLDGRVKTLHPKVHAGILAIRDNADHMKQLEELGIDTIDMVVINL
                     YPFKQTIHKEGVRLEEAIENIDIGGPTMLRSAAKNYQDVVVVVDPADYPKVIEEIEAT
                     GDVSRETKFRLAYKVFEHTSHYDTLIAAYLRSKLGDVSFPKTLTLTFEKVQDMRYGEN
                     PHQNAVFYKEVGSGKGCLTGAKQLHGKELSFNNINDANGALELLKEFDEPTVVVTKHA
                     NPCGVASGADIFDAYVRAFEADPVSAFGGIVALNREVDARTAEEIKKIFYEIIIAPSF
                     SPEAIEILGTKKNLRLLQIEDIMAKQPAGTYDMKKVSGGLLVQDCDSILIDPENLKCV
                     TGTKPTDQQMEDLAFAMKVVKHTKSNAIVLAKNKQTIGVGPGQTNRVTALRIAIDYYG
                     KDKTPGSVMASDAFFPFSDCVEAAHEAGITAIIQPGGSIRDQESIDACNKYGIAMVFT
                     GIRHFKH"
     gene            complement(9577..10200)
                     /gene="purN"
                     /locus_tag="PLG72_04865"
     CDS             complement(9577..10200)
                     /gene="purN"
                     /locus_tag="PLG72_04865"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOJ80163.1"
                     /translation="MFKLGVMVSGGGTNLQAIIDKIKSGYLEDCTIVTVVSSRQNAYA
                     LERAKNNDIPAVCISKKSFASEEEYDRALIEHFRKYDVDLVVLAGFLSMLGRTFVETY
                     RNRIINVHPSLIPSFCGKGYYGIIPHQKALEYGVRVTGATVHFVDYEYDSGPIILQKA
                     VEVMDDDTPESLQLRVMQQAEWELLPEAIKLFKEGRLVVEGRKVRII"
     gene            complement(10312..>11210)
                     /gene="purM"
                     /locus_tag="PLG72_04870"
     CDS             complement(10312..>11210)
                     /gene="purM"
                     /locus_tag="PLG72_04870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOJ80164.1"
                     /translation="DAVAMCVNDIVCGGAEPLFFLDYIACGKNKPEKIAQIVKGVADG
                     CVMAGCALIGGETAEMPGFYPEDEYDLAGFAVGIVDKRKIIDGRDIREGDKLVGLASS
                     GLHSNGYSLVRKLLNPNKEKLSEYVGLLNSTLGEELLKPTRIYVKSILAVKGKYNIKG
                     IANITGGGFIENIPRMIPDGLRARIDIGTWPVQPIFTLLQEIGKITVESIYNTFNMGI
                     GMVLAVDPDEADGAVAYLNSIGENAYIIGEIIRGEKGVELCEPGKAVTVCGKVEDSTE
                     GGSNENRPECGADGEGPANSNG"
BASE COUNT         2395 a         3064 c         2685 g         3066 t
ORIGIN      
        1 gttggatacg gccatcttgt accatctgtc tccgaacgct cccacggaga acacggcggc
       61 acgtctgacg gtacaggaac gtatcgctcc ttccatcagg ccgcttccgg atgacgtcga
      121 aagcaggatc atgtttttgg tgtacatcag tttctggagc ttttcagtta tgctcctttg
      181 gagtgccgaa gcttccttcg tcctgtggcc tatcatcggg gtggccattt tttggagaac
      241 gtcatccctg acttccacag gtccgggtat gaagagtttc ttgtgcatat ccattcctcc
      301 gcattataaa ataaaaagtc aaatctttga taagacctga cctccatgca tgcatacatc
      361 atgcactcgt taagctctgt ccttttgcct gagagtttag cttttcagcc ttgccccttc
      421 ggcgccgtca tggtctctcc agagatccgt ccagacaagg tcgctgccgc ttcctcgcca
      481 ttcatccggg atatttaatt ttgctgctct accggctgtc ccgccccagg atgtctcgct
      541 aaaaaagata gctatattat aaaatctata tacaatcatt gtcaaggaat tatcaaaaat
      601 ttactgtggt actgggacgg tttcatgacg cagcaaagtg aggtctgttt tgtctgaccc
      661 tgttatgtca aaaagttgag aactgtcccc catcaaagag ccgttacctt gacccgggac
      721 aagagtggga cacgggaacc gtctgcgtat cttgtccgcc ttttcattca tcagcaagta
      781 tattatactt aaataggctg tgcattgacg cactgctcaa agaaatcatc atttgatgca
      841 ggatttttct gcctattttt ggttcaggag catatatgcc attcaaactt acaaggccaa
      901 ataatcagga cgagccggca aaggccggta tgcaggacaa accgacgatt accgtgtcgg
      961 tccgtgaact gctcgaattc gccatgcagt caggcgacct ccgcaccgcg tcatattccc
     1021 cttcaagggc tgccgaaggc accaggggcc atgcagagat cagagcaatt ataaaacaga
     1081 tgttgtccga tgatgccata tatgaaaccg aggtcccgat aactttccgg ttggagggcg
     1141 acaacctggt acttgagata acaggcagga tagacgggct tttgaaggat gcagccggtg
     1201 acactgtcca tgagatcaaa acaacgatgc ttccgcttga gcagatagaa catgaccacg
     1261 accctcagca ttgggcgcag gtaaaatgtt atgcttatat gtacgcccgt atgcatggtc
     1321 tggaccgtat agccataatg ctgacctact accagttgga tgaaaaacag gagaaatcct
     1381 ttacagacat ttatgatatc gattccctgg aatcattttt catgccgttg gccggagact
     1441 atatggcctg gcaggaaaag atgaacaact gggctatcgt aagggaccgc tccatacatc
     1501 agttggattt cccctatccg gaacgccgta aaggccagca ggaactaatg gacgacgtat
     1561 atgacacgat cggggtcagg ggactgcttt tcgcgcaggc gcccaccggg acagggaaaa
     1621 ccgtagccgt tctctttccg tcagtcaagg cattgggcga cagactggta tcaaagatct
     1681 tctatcttac cgcaaaaacc acaacaagaa gccttgccga gaagtctttg gaagatatgc
     1741 gcaaaaaggg gctccgtata aagtcgataa caataacggc aaaagaaaag gtgtgcttta
     1801 atggtacaca tttctgcaac ccggagatct gcatgtataa agaggattat tacgggaaga
     1861 taaaaaaggc tctcgaggat gcattgcagg aagacgcgct tgaccggacc gttatagaga
     1921 aatatgcctc aaaatactcc atatgtccct tcgaactgac gctggacctt tcgcttttat
     1981 gcgatgtgat catatgtgac tacaattatc tctatgaccc tagggtcaaa ttgtccaggt
     2041 acttttcaaa acgcgggaat tactgctttc ttgtggatga agcccacaac ctggtcgaca
     2101 gggccaggga catgtactcg gcggagatat ccaggcgctc gtatttcagg ctgaaaaagc
     2161 aggcaaagga agagttgccg gaaatatatg acgcgcttga cagaatgcac aagtgttttc
     2221 ttgatatttc aaaggcactg gctgaagaca gcggcgatca aaagaaaggc ttttatctcg
     2281 tggagagggc atatcccgat gagctctgca ggccgatcga gcgcttcaca ggcttagtcg
     2341 gagacttgct gccgaggatc ctggcagtat cgttcatgga tcagctcctt gaagtctact
     2401 tcgacagcct tcattttttg aggatttcag aattgtacga tgaaaggtat gtgacgtgtt
     2461 atgataagtc ggcgacagat ttcaaaataa agctgttttg cgtggacccg tcaaaactgc
     2521 ttcgcgatgc catgaacaaa ggacgggcat ccatactgtt ctcggcgacc ctctccccca
     2581 tcgggtactt cacatcgatg ctcggcggcg gggatgacgc ccgtgtaacg gttatacctt
     2641 cgcctttccc cagggaaaac ctctgcgttt atatcgatga ttccatatcg acgaagtaca
     2701 aaacaagaat gttttcttac gacaggatcg cggaaacgat actggaaacc gtatccgcca
     2761 ggacaggcaa ctatatggca ttctttcctt cctttgaata tctcaacgag gtctattaca
     2821 ggttcatggg cataagccgc ggcatccgct cgctatgcca gactcccggg atgtctgagg
     2881 ccgcaaggca ggagttcctg gatgagtttg aaaaaacagg agaaacaagc ctcgtaggat
     2941 tcgcagtgat gggcggcgtc ttcagcgagg gcatcgattt ggccggagac aggctttccg
     3001 gagcgatcat tgtcggtgta gggcttccac agatatgcaa tgaaaggaat atcatccggg
     3061 cacactttga cgagcggtcg gggaacgggt ttgaatatgc atatatatat ccgggcataa
     3121 accgggtgct gcaggctgcg ggacgcgtga tacggacaga ggacgacatg ggcgtcgtta
     3181 tattgctgga cgaacgcttt tcgcactaca actacaggga actgcttccg ccggagtgga
     3241 cccctatcca gaaaatgtcg gacggtatcc tgctcacgga tgttttgcag gacttctggg
     3301 gttgagcgcc caccggcggc actaaagcac gggaacggtc ctcctgcctc aggcaagaga
     3361 accgtccaat gtccgtaaag atcatctttt aaataaagcc gcgcatcagg tcttcgaacg
     3421 tgtcgcgcct gcggatcagc ctgggactgc cgtcaaggcc tataagcact tccgccggcc
     3481 taaggcggtt gttgtagttg gacgacatgg aataaccata cgctccggca tccattacgc
     3541 cgatgatatc cccttcggct gcttccggca gttccctgtc gttggctatt atgtcgccgc
     3601 tttcgcagat attccccaca acggtcgcct tccggaccgt gtctgtcttt agtacagcgt
     3661 cgttccggta aaactcgata tcgtggtgag aatcgtacat ggcaggacgg atcagcacgt
     3721 tgaagccgac atccgttcct atatacgttt tgctgccgtt ctgcttcact gcatgcaccg
     3781 tgcccagcag gacgccgcat tccgcgacga cgtatcggcc cggttctatc ttcagcctga
     3841 ccctcttccc atagttcgcc gtccagtcct ccatcaactg tgagagcccg cggcccattt
     3901 cctcaagatc cagcctctgc tgcccttcct gcttgcggta tgggatgccg aatccgccgc
     3961 ccatgtctat gaattcgaga tcctcaaaat tggaagcaat gttcagcagg gctcttgcgg
     4021 cctgcagata gcttgacggc tccatgaaca gcgagccaat gtgctggttt atcccagcga
     4081 ggctgagcct gtattccttc agtattgctt tcacttcgtc catcatttcc aggttgatgc
     4141 cgaacttggt gttttcgccg cctgtgacca cttttgcatg atggcccgct cctacgcccg
     4201 gattgaagcg gactgcgacc tttcctcccg gattgagcct tccgtactgt ctcaactggg
     4261 acagcgagtc acagcttacc aggacgcctt tgtcgatggc atactgcatt tcttcggcag
     4321 aaacattgtt ggccacgaac aatatctcat ccggcctgta tcctgcagcc agcaggacat
     4381 gtatctctcc gggcgacatg gcgtccgcgt ggagcccttc atcccggatt attttcagca
     4441 gttccaggtt cgaattggcc tttatcgagt agttggatct gtagttggaa tacgagacga
     4501 gtccggccaa ctcacggcac ctttgcctga gtatcgactc attgtagaca tacagcggac
     4561 tgccgtattc cctgatcagt tcggacggtt tgctgctgcc atagaaattt gtctgttcgg
     4621 taaagtatac cttcatgccg tttcccccat ctactttagt atatattatg tcgacacatg
     4681 tttttgttat attgtcctgt atgtgtcttt tatatcgttt ctgatcatcg ttaagggtgt
     4741 gttggctctg tcccgtttat ccgaatggcc ttccgtccct gaggaagtta atgatgcata
     4801 tgatcagcaa tgtcagcagc agtatcgtac tgaatgtata gaacagcatg cccgcaacgg
     4861 attttttctt tttccctgcc atagaactga cacctggccc cctctttttg actgccagcc
     4921 gatcctgcca tgtgatattc actgatgtaa ctgtttccgg cgcctgctgt caatgttcct
     4981 gatcctgctg ctctctctct ttcagctttt ggagcgccca gttccttgtc agcaccgacc
     5041 ttgggtgcag cggctgccct tttttcaaaa catgccttat ggtgctgttt atccccgcca
     5101 caatggcttt ctcgaggctt tcttccgccg ctttccttat ttcatcgacg ccttcgaagt
     5161 ccctgccggg ttcgatatag tccgcgatga aaacgatgct gcttatgtcg tccatccccg
     5221 gcctgcccag cgtatggtcg gctaccgcgg caagtatctg cggatcatcc acgccgaaca
     5281 gttcccttgc catgtgggcg ccgatctctc catgcagcag tttcggctgc gatctcgata
     5341 tttcatcgac aatgatacca tacttctcac atatcggtaa aacggcatct ttatccaggt
     5401 ccttggcaca gtcgtggata agtccggcga ccgcagcctt gtcaacatcg gcgccgtatt
     5461 tttccgccag ccggatgcac gtttccatcg tgtttatcga gtgcctgaac ctgtgcggtg
     5521 tgagcatatg ctgcatcttt ttcatcattt catccagatt gagcatcttc catgttaccc
     5581 tcttgtgtca gtagcgtttt agtaccatct tcaatgtata caggcttctg tataagtcct
     5641 ggcatatatc agcccctgta cagacccttt tcatatatat accgttcgac ggacccggga
     5701 acaaggtatt ttatggaccg ccccttcctg cacctttcgc gtatatccgt ggaggagata
     5761 tctaccaggt gtgacgggac aggatgtatc ctggcgcctt ttcccgtgga aatccgatgt
     5821 atcgcttcat tgaagccggc ttcatcatag cccgggcgca gtaccgctat gaactcacag
     5881 agccggaata ctttctcgta ttccctccat gtaaccagct catgaactac gtccgcgcct
     5941 acgatgaaat atatggcggt ttcctcaccg taatcctccc tgagttcctg caaagtgttt
     6001 atagtatacg tcaggccgtt cctgtctatc tctatgcgcg aggcctcaaa ataagggttg
     6061 gaactgacgg caagcctcac catctcgaag cggtgctccg catctgccac ctcacagccc
     6121 ggcttgtgcg gcggctgccc cgaagggatg aacagtacct tgtccaggcc tgccttttcg
     6181 cggacatctt ctgcgattat aaggtggccg ttgtggatcg ggttgaacgt cccgcccatt
     6241 atgccgatcc tttgtggttt ccggtccggt gccccgggct tgcattgcgt cccggtacta
     6301 tatcttgccc cggtcttata ttgcgtgccg gacatgtatt cggttttgcc agtcccgttc
     6361 ctgttttcca taaccatttc cttacgatcg atcagggaat atgcaactgc cgtacagcca
     6421 tgattgcagc atttgtcata cagcggcacg aactgctgtg ataatccttt agttgccatc
     6481 catgtacgct gcaacagatt tttcctgtat tacttcttaa taccgatatc tcttcggtaa
     6541 tgcatgcctt cgaacttgat ctttccgacc gcggcatacg cttttgcgcg cgcatcgtcg
     6601 atggtgtccg ccacggccgt gactccaagg actctgccgc ccgccgtata atacttaccc
     6661 gcttccagct tcgttcccgc atggaaaact atcaccgacg gatctttctc agcttcatct
     6721 atacccgtga tttccagtcc ggtctcatat ttgcccggat atccgccgga agccagtatc
     6781 acacagacag cggacctgtt ctcccattcg atgtttatct ctgacagcct ttcatcgatg
     6841 atcgcttcga atatgtcaat aatatctgtc ttaagatgcg gcaggaccac ctgggtctca
     6901 ggatcgccga accttgcgtt gtattcgagc accttcggcc cgtctgccgt gaggatcagg
     6961 ccgaaataca gcacgccctt gaacctgcgg ccttcttttc tcatcgcttc tattgtgggc
     7021 atgaatatgt ttttcatgca gtaatcgttg atttcggcag tataaagcct gcttggcgag
     7081 aatgtgccca tgccgcccgt gttaggcccc atatcgttgt ccatggcacg tttgtggtcc
     7141 tgggaactga ccatcgggac cagcgtctcg ccgtcagtga aagcgaggat cgaaacctcg
     7201 gtgccgacca ggaactcctc tatgacgacc ctgttgcctg cacttccgaa tatcctgtcg
     7261 gacatgatgc tgcgtactgc cttttcagct tcatcgtaat tgcccgctat gatcacgcct
     7321 ttccccagcg caagcccgtc agccttcacg accgttgggt aactgcatgt cctgaggtat
     7381 tcaagagcgt tatcggcatt atcgaacacc tcgtatgcgg cggtagggat gccgtatttc
     7441 ttcatgaggt tctttgagaa caccttgctg ctctcgatct cggcagcagc ctttacaggc
     7501 ccgaatgcac ggatgcccgc attttccagg gcgtcgacca tgcccgccgc aagaggatcg
     7561 tccggcgcca cgactacgag atctatccct tcgcttttgg caaagctggt gatgccttca
     7621 atatccattg cctttattgg tacacactgc gccagcgacg caatgcctcc gttgcccggc
     7681 gcacagtaga gcttgctcac tttcgggctc tgtgatattt tccatgcgat ggcgtgttca
     7741 cggccgcctc cgccaacgat aagtaccttc atctttccac tccccgtctg aacgatttca
     7801 tcgcacactt acgcaaatgc cctgagatcc atttacaggg gaccgttgcc ttgtctggaa
     7861 gccgagaact gtcccttctg ttcaaaaggt gagaactgtc cccgtatctc agctcagtgt
     7921 ttgaagtggc gtatgccggt gaataccatt gcgatgccgt acttgttgca tgcgtcgatg
     7981 gattcctggt ccctgatgga gccgccgggc tgtattatgg ctgtgatgcc ggcctcatga
     8041 gccgcttcga cgcagtcgga gaacgggaag aatgcgtcgg aggccatgac ggaacccggt
     8101 gtcttgtcct ttccgtaata gtcgattgct atgcgtaatg ccgtgaccct gttggtctgg
     8161 cccggtccta cgcctatggt ctgtttgttc tttgccagca cgatcgcgtt ggacttggtg
     8221 tgcttgacta ccttcattgc aaaggccagg tcttccatct gctggtcggt gggctttgta
     8281 ccggtgacgc atttcaggtt ttcagggtca atgaggatgc tgtcgcaatc ctgcacgagc
     8341 agtccgcccg agaccttctt catgtcatat gtccctgccg gctgttttgc catgatgtct
     8401 tcgatctgca gcagcctgag gtttttcttc gtcccgagta tctcaatggc ttccgggctg
     8461 aatgaaggcg cgattattat ttcatagaat attttcttta tttcttccgc ggtccttgca
     8521 tcgacttccc tgttcaacgc cacgatcccg ccgaaggctg atacgggatc ggcttcgaat
     8581 gccctgacat atgcatcgaa tatgtcggcg ccgctggcca caccgcatgg attggcatgc
     8641 tttgtaacta ccaccgtggg ctcgtcgaac tctttcagaa gctcaagcgc tccgttggcg
     8701 tcatttatat tattgaagga aagttccttg ccgtggagct gcttcgcacc ggtaaggcag
     8761 cctttgccgg atccgacttc tttataaaag acagcattct ggtgagggtt ttccccgtat
     8821 ctcatatcct gtactttttc gaatgtcagc gtcaatgttt tggggaacga cacatctccc
     8881 agtttgctcc tgagatacgc ggcaatgagt gtgtcgtaat ggctggtgtg ctcgaatacc
     8941 ttgtatgcca gcctgaactt ggtctccctg gaaacgtccc cggtcgcctc gatctcttcg
     9001 atcactttgg gataatccgc gggatccaca acgacgacta catcctggta gttcttcgct
     9061 gcggatctca gcattgtggg accgcctatg tcaatgttct cgatggcttc ttccagcctg
     9121 acgccttcct tgtggatcgt ctgcttgaaa ggataaaggt tgatgaccac catatctata
     9181 gtgtctatac caagttcctc caactgcttc atgtggtctg cgttgtcacg tatagccagg
     9241 atcccggcat gcactttcgg atgcagcgtc tttaccctgc cgtcgaggca ttccggaaat
     9301 ccggtgatct cagagatacc aatgactttg atgcccgcat ctgcaagagt tttgtatgtt
     9361 cctcctgtcg atattatctc aaccccgcgc ccggcaaggg cctgagccag ctcaacaacg
     9421 ccggttttgt cggatacgct gatcaatgca cgtttaatca ttccgtttcc cttcctttat
     9481 atataataat ataactttac taacacatgt acaggacatg gatctttgtc cacatacatt
     9541 gccatggcaa tgatgtacgg gtaacattat cccttgctat atgatcctga ccttcctgcc
     9601 ctcgacaacg agccggcctt ccttgaacag ctttatcgct tccggcagca gttcccactc
     9661 cgcctgctgc atgactctca gctgcagcga ttccggtgtg tcgtcatcca ttacttcaac
     9721 ggctttttgc agtattattg gcccgctgtc gtattcatag tctacaaaat ggaccgtggc
     9781 acccgtgacc ctgacaccgt actcgagggc tttctggtgc ggtattatcc cataatagcc
     9841 tttaccgcag aatgacggga tcagcgacgg gtggacgttg atgatcctgt tccggtatgt
     9901 ttcgacgaaa gttctgccca gcatcgacag gaaacctgcg agaactacca ggtccacatc
     9961 gtatttccgg aaatgttcta tcagcgccct gtcgtactct tcttccgaag cgaaactctt
    10021 tttcgaaatg catacagcgg ggatatcgtt gttttttgcc cgctccagcg catatgcgtt
    10081 ctgcctgctg gaaacgacgg tgacaattgt gcagtcttca agatatccgc tcttgatttt
    10141 gtctatgatc gcctgcaggt tggtaccgcc gccggatacc atcactccga gcttaaacat
    10201 attcagaacc agcctttcct ttatccaggg atccggataa tggatccaaa atctgctctt
    10261 ccagcggttc ttccgctgtt acatcagttc tcctgtcagc acccgttcct tttatccatt
    10321 ggaatttgcc gggccttctc cgtccgcacc gcattcgggt ctgttttcat tcgatccgcc
    10381 ttctgtgctg tcttcgacct ttccgcatac agtgactgct ttccctggtt cgcacagttc
    10441 cactcctttt tctcccctga tgatctcgcc tatgatatag gcattttctc cgatcgaatt
    10501 gagatatgcg actgcgccgt ctgcttcgtc cgggtccacc gcaagcacca ttcctatgcc
    10561 catgttgaac gtgttgtaga tgctttccac cgtgatcttg cctatttcct ggagcaacgt
    10621 gaatatcggc tgcacgggcc atgtgcctat atctatcctt gcccgcagcc cgtccgggat
    10681 catcctgggt atgttctcaa taaatccgcc gccggtaatg tttgcaatgc cttttatatt
    10741 gtactttccc tttacagcga gtattgactt gacatagatc ctggtaggtt tcaggagttc
    10801 ctcgccaagg gtcgaattga gcagcccgac atattcgctc agtttttcct tgttggggtt
    10861 gagaagcttt ctgaccagcg aataaccgtt gctgtgcagg ccggaagatg caaggcctac
    10921 cagcttgtcc ccttccctta tgtctcttcc gtcgattatt tttctcttat ccactatgcc
    10981 gactgcaaaa ccggccaggt catattcatc ctccgggtaa aatccgggca tctctgcggt
    11041 ctctccgcct atgagggcgc atcccgccat tacgcagccg tcagccacac cttttacgat
    11101 ctgtgcgatc ttttcaggct tgtttttgcc gcaggctatg taatcgagga aaaacagcgg
    11161 ctcagcccct ccgcatacga tatcgttgac gcacatggct acagcgtcta
//
LOCUS       DAOSCC010000062        11170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3887_length_11170_cov_5.302924, whole genome shotgun sequence.
ACCESSION   DAOSCC010000062 DAOSCC010000000
VERSION     DAOSCC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3887_length_11170_cov_5.302924"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..646
                     /locus_tag="PLG72_04875"
     CDS             359..646
                     /locus_tag="PLG72_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80165.1"
                     /translation="MAEKAKHQQDFEKTLRLYLKQAREKLNGDLAGTREAIRLIAADK
                     TRSFIEMTDRGLSKEERELLKSLIMTSMRQSFCYGYGIGKFEGNTNERVYL"
     gene            1065..2762
                     /gene="ilvB"
                     /locus_tag="PLG72_04880"
     CDS             1065..2762
                     /gene="ilvB"
                     /locus_tag="PLG72_04880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ80166.1"
                     /translation="MKMTGAEAIIKALETENVDTIFGYPGAAISPFYDALLDSNIKHI
                     LTRNEQGAAHAANGYARVTGRVGVCVATSGPGATNLITGIANSYMDSVPIVAITGQVR
                     SDMIGTDAFQEADITGATAPFSKHNYLVKKAEELPRIIKEAFHIASTGRQGPVVIDIP
                     VDIQNAVIDFSYPENIEILGYKPTIKGHPGQIRKTASALHAAKRPVICAGGGLITADA
                     SGEMLELAERRNIPVVTTLMGIGSIPSDHPLYFGMVGSHGSPTANYVLHHADLVMILG
                     ARVGDRAIGAASALEKKAKIIHIDIDPAEIGKNIDVAVPVVGDLKQVLTALLELVEEG
                     GSTEWLETAAEVRRKNGKRPEQEACGKKTEKDAGTDGKMAEDGQVAPDTGYVNPKYLL
                     SILSDYLPEDAIVTTEVGQNQIWAANNYKVKKPRTFITSGGLGTMGYGLPSAVGAKVG
                     RPGSTVAVISGDGSFQMSMQELGTMKQYGIDVKIVLFNNRRLGMVRELQLTRHGGRYS
                     QVFLDSNPDFVALAAAYGFRGERISSDAQVREALERMTACSGPYILECMVDPMEPTL"
     gene            2975..3499
                     /gene="ilvN"
                     /locus_tag="PLG72_04885"
     CDS             2975..3499
                     /gene="ilvN"
                     /locus_tag="PLG72_04885"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ80167.1"
                     /translation="MAKHILSVLVENHAGVLSRVAGLFSRRGFNIDSLAVGITENPEV
                     SRMTIVVDGDEHTVEQVSKQLNKLIDVIKIKRLDKHESISRELALIKVSANAATRSEI
                     VQLVEIFRAKIVDVSKSTLTIEASGSEDKVAALEDLLKPFGIKEVVRTGVIAIERGNR
                     IIKITNNEESDSNG"
     gene            3492..4487
                     /gene="ilvC"
                     /locus_tag="PLG72_04890"
     CDS             3492..4487
                     /gene="ilvC"
                     /locus_tag="PLG72_04890"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOJ80168.1"
                     /translation="MAKMYYDSDCSLGLLKGKTVAVIGYGSQGHAHAQNLKDSGINVV
                     VGLAPGSARRKQAEEDGLTVMDTAEAAKAGDLIMILTPDQTQAEIYSRDIEPNLKDGD
                     ILAFAHGFNIHFNQIKPPEYVDVIMIAPKGPGHTVRSQYLEGRGVPALIAVHQDASGK
                     AKDYALAYAAGIGAGRAGILETTFREETETDLFGEQAVLCGGVTELMKAGFETLVEAG
                     YQPEIAYFECIHEMKLIVDLINQGGFSYMRYSISDTAEYGDYVTGKRIITEETRKEMK
                     KVLSEIQSGEFASKWITENKAGGRAQFLATRARESEHQLEKVGRELRKMMSWLKK"
     gene            4577..6121
                     /locus_tag="PLG72_04895"
     CDS             4577..6121
                     /locus_tag="PLG72_04895"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ80169.1"
                     /translation="MSARIRIFDTTLRDGEQTPGVSLNIQEKLEIARQLAKLGVDIIE
                     AGFAISSPGDFMAVKTVAENVKGPVIASLSRAVEKDIDRAWEALQNAENPRIHTFIAT
                     SDIHMKYKLKMTEEEVLERAAAMVRYAKKYCSDVEFSAEDASRTRTEFLYRVLEEVIK
                     AGATVVNIPDTVGYAMPDEFGRLIKGIKENVRGIEKVDISVHCHNDLGLAVANSLSAV
                     MNGATQVECTINGMGERAGNASMEEIIMGIQTRKDFYSGMQHNIDTRQIYRTSKLVSK
                     LTGVNLQPHKAIVGKNAFAHESGIHQHGVLAERSTYEIMTPESIGLSKNRIVLGKLSG
                     RHAFEERLKEMGYNLPPEEVTKAFEKFKELADKKKHVTDRDIEALIGEKVSEVEEVYK
                     LDSFQINSGNRVIATATVSLFKNGELMTEAATGDGPINAAFKAIERSVGFEVHLEDYS
                     LKGVTEGADALGEATVRVMKDNRIYVGRGVSTDVIEASVKAYINAINRIIHDHGKETS
                     GGRETI"
     gene            6118..7692
                     /gene="cimA"
                     /locus_tag="PLG72_04900"
     CDS             6118..7692
                     /gene="cimA"
                     /locus_tag="PLG72_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOJ80170.1"
                     /translation="MKKVVVLDSTLRDGAQALGISFTVEDKLKIVRQLDKLGVAYIEA
                     GNPGSNPKDLEFFERGSRLKLKNSKLIAFGSTRRVGIPVDEDANVRSLLKAGTDAVVI
                     FGKSWDFQVTEILKTTFDENLRMITDTVSYFRKLGKEVVYDAEHFFDGYFSNPEYAME
                     TLKAAAEAGADCLCLCDTKGGCLPMDVYEITKKVVEQFDVPIGIHTHNDMGMAVASTI
                     MAVQAGATQIQGTINGFGERCGNANLCTIIPTLQLKMGYECIPRESMHEITPVARAVS
                     EIANVVHDERAPYVGRSAFAHKAGMHADAVVKNTFAYELLDPAEVGNRRTFLMSEVAG
                     RSAVLNLIQKVDPSITKDSPETRQILDRLKEMEHQGYQYEGAESSFELIIRKMLGKYK
                     PFFELKDFKVIVTEPAKRGEWNSSAMIKVKVGDQEEITAAEGIGPVNALDNAVRKALS
                     RFYPVINRMKLTDYKVRVLDSESATAAKVRVLIESTDGNEVWTTIGVSTDIIEASWKA
                     LADSIEYKLGNGHTAE"
     gene            7721..7987
                     /locus_tag="PLG72_04905"
     CDS             7721..7987
                     /locus_tag="PLG72_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80171.1"
                     /translation="MTLKGKIIIDGKIAREGASYDNDSAHSFRDKLENCLVDLCADLE
                     IPVPLWLKKNTRELAVFRKTFFSSEQFIEKVWFDKFEIVLSAEE"
     gene            8296..8634
                     /locus_tag="PLG72_04910"
     CDS             8296..8634
                     /locus_tag="PLG72_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80172.1"
                     /translation="MTIDYYIFGGFAVQAQVLVNTYLSIKTMKKGFIAVVLLNILGIC
                     FAGIGSVIMSGQMAALPGVVTYFSSTVISTVLYYYKKGLRDNIQRLTEQRDEITSLYR
                     EISASKEKLS"
     gene            8668..9978
                     /locus_tag="PLG72_04915"
     CDS             8668..9978
                     /locus_tag="PLG72_04915"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOJ80173.1"
                     /translation="MKENEKKLERLAYYDPLTGLPNRKMIIQKLDMLIHYSERSQEGF
                     ALVYIDIDNFKEINDLMGHHIGDMLLKEVTKRWKVRLNKKDVLGRMEGDEFAIIIRRE
                     LSDEDLLRYVESFRDAISDVFSYGMKELRIEASFGISVYPKDGRTTEDLLKHAGMAQA
                     YVKASGKKGIEFFRHEMQKNMIERVQLEDGLKSAILNRELFVVFQPQYSCETQKIRGF
                     EALVRWRSKKLGMVSPATFIPIAEETGLIIDIGEWILRTVINKFIKVKAANRDYILTI
                     NISVVQIIQPSFVSMVKKILDETGFDGSRLEFEITESVFISYPEKVINTLKQLKEMGI
                     SIALDDFGTGYASLKYLQMLPIDTLKIDRTFVSGIGEMESGNQIVGSIIALAHSLGIL
                     VVAEGVENMDQMNYLKEQKCDCVQGFLLSMPLEGDQVDQLIATA"
     gene            10248..10949
                     /locus_tag="PLG72_04920"
     CDS             10248..10949
                     /locus_tag="PLG72_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOJ80174.1"
                     /translation="MTRFKFFAVYFFIVLMLLSAIQIIIDAGEEKARNAAVLDEKREY
                     TIFIEIEDKTLYLLDGNTCVKEYRIASGKSGYPSPLGCWTIIEKGDWGEGFGGRWLGL
                     DVPWGKYGIHGTRESGSIGTAASHGCIRMFNDDVAELYSLVEIGTEVVIVNGQFGPFG
                     RKFDEINPGDRGADVLAIQRRLKQLGYYNGPLDGIYEDDMKKAVHQFQRGTGLKAQNT
                     IDRQTWLNMGFREFE"
BASE COUNT         3163 a         2487 c         3169 g         2351 t
ORIGIN      
        1 tttgggaggg cggcctgtct tacattggag ttgcttcgct aacatctgct ggccagctca
       61 tttgctccaa tgagggcgca ggtcactcgc cgtccatggc tcgggaccag cgccggcagc
      121 gtccgtgctg ccggtttttg tacctggtgc ctcattcgag ctgcatgacc cggccgcatc
      181 tgtagcactt gcataccatc aatggctgta tcggctgccc ccttgtaaca ctgggccgga
      241 tctgtcttca gcgtaacccg gacttcatcc ggaaggatga ccggctgagc catcgctgga
      301 cgacgtatag ttccatatgt gtattggcaa cacttacttg taacaggagg tggttgccat
      361 ggctgaaaaa gccaaacatc agcaggattt cgaaaaaaca ctgagacttt accttaaaca
      421 ggccagggaa aagcttaacg gggatctggc cggtaccagg gaggccataa ggctcattgc
      481 cgctgacaag accaggagtt ttattgaaat gacggacagg ggcctcagca aggaagaaag
      541 ggaactgctg aagtccctga tcatgaccag tatgcgccag tctttctgct acggatacgg
      601 catcggaaaa tttgagggca ataccaacga gcgagtatac ctttaactgc aataccgcaa
      661 aaaagcataa aaaagacggc tcaagagggc aataaagatt aaaaaataat attgacactc
      721 cgttgaatgt gctgatacaa tattatacaa tcatctttca gaccggataa aaagcaatga
      781 aggaaaaaag gcaggtctga ctgcgcaaga gagccggtgg gtggtgcgaa ccggcgcata
      841 ggaccgtgcg tatagctcat tccggagctt cttcgctgaa ccctgaaatc tacaggatcc
      901 aggcggacga cgttgaccac gttacagtca agtactcaaa gtactactga gggtaagcat
      961 ttgcttatcg aatcagggtg gtaccacgtt ggatgttccc ttcgtccctg cagcgatgca
     1021 gcgacgaggg tttttttata cagcggcata gcggagggaa atagatgaaa atgacaggtg
     1081 cagaagctat cataaaggct ttggaaaccg aaaatgtcga taccattttc ggctatcccg
     1141 gtgctgccat ctccccgttt tatgacgcat tgctggacag caatataaag cacatactta
     1201 cacgcaacga acagggagcg gcacatgcag ccaacggcta tgccagggtt acgggcaggg
     1261 tcggagtctg cgtggcgacc tcagggcccg gtgcgacgaa ccttatcacc ggtatcgcca
     1321 actcatatat ggacagcgtt ccgatagttg cgatcaccgg ccaggtaagg agcgacatga
     1381 tcggaacgga tgcattccag gaggctgaca taaccggggc gaccgctcct ttcagcaagc
     1441 ataactacct tgtaaaaaaa gccgaggaac tgcccagaat aataaaggaa gcattccata
     1501 tagcatcgac gggcaggcag ggacctgttg tcattgatat acccgtcgac atacagaacg
     1561 ccgttataga tttcagttat ccggaaaaca tagagatctt agggtacaaa ccaaccataa
     1621 aaggccatcc gggacagata aggaagacgg cttcggcgct ccatgcggca aaaaggcctg
     1681 tcatatgtgc cggaggaggc ctgatcactg ccgatgcctc gggcgagatg cttgaactgg
     1741 cagagaggcg gaacataccc gtagttacga cgttgatggg gattggatcc ataccgtcgg
     1801 accatcctct ctatttcggc atggtaggct cccatgggag ccctacggcc aattatgtac
     1861 tgcaccatgc cgatctcgtt atgatactcg gggcaagggt cggcgacagg gcgatcggcg
     1921 ctgcaagcgc actggagaaa aaggcaaaga tcatccatat cgacatagat cctgccgaga
     1981 taggcaaaaa catcgatgtc gcagtgccgg tggtaggaga cctgaagcaa gtcctgacgg
     2041 cattgctcga gcttgtcgaa gaaggcggga gcactgagtg gcttgaaacg gcagctgaag
     2101 tgagacgcaa aaacggaaaa cggccggaac aggaagcatg cgggaaaaag acggagaaag
     2161 acgccggtac ggacggaaag atggctgagg acggacaggt tgcgccggat accggttacg
     2221 tgaacccgaa atacctgctg tcgatacttt cggattactt accggaggat gccatagtga
     2281 cgaccgaggt cggacagaac cagatctggg ctgccaataa ctataaagtg aaaaagccca
     2341 ggacattcat cacttcaggc gggcttggga ccatgggata cggtcttccc tcggccgtcg
     2401 gggcaaaagt cggaaggcct ggttccaccg tggctgtcat cagcggggac gggagtttcc
     2461 agatgtcgat gcaggaactc ggcactatga aacagtatgg catcgatgtg aagatcgtcc
     2521 tgttcaacaa ccgccgtttg ggcatggtga gggaactcca gctgacaagg cacggagggc
     2581 ggtattcgca ggtgttcctc gacagcaatc cggattttgt ggcgttggct gcggcttacg
     2641 gcttcagggg cgagaggata tcatcggatg cacaggtccg ggaagcgctg gaaaggatga
     2701 ccgcctgttc cggaccgtat atcctggaat gcatggtgga cccgatggag ccgaccctgt
     2761 gaagacggcg gaaagactgg cgggaatatt gccttgccca tgcagacttg gcagtatatg
     2821 ctgctgaata ctggcatcgg gatcagcgta gatcgtgtat ggcagagaga actgagcaca
     2881 gtgataagaa cttttcagga cagaaataaa aagtatcgtg tttcatgatc attgaatatc
     2941 gggcgtcatg cctggcacga aaggggtagg gattatggcc aaacacatat tgtcggtgct
     3001 ggtggaaaac catgccggtg tactgtcgag agtggccgga ctgttcagca gaagggggtt
     3061 caacatcgac agccttgcgg tcggcataac tgaaaaccct gaagtctcaa ggatgaccat
     3121 agtggtggac ggagacgaac atacggtaga acaggtaagc aagcagctta acaagctgat
     3181 agatgtgatc aagataaaaa ggctggacaa acatgaatcg atttcccgcg aacttgcgct
     3241 gatcaaggtt tcagccaatg ctgcgaccag gagcgagata gtccagctgg tggaaatatt
     3301 cagggcaaag atagtggacg tgtcaaagtc cacgctcact atcgaggcgt ccggaagtga
     3361 agataaggta gcggcgctgg aggatctgct caaaccgttc ggcatcaagg aagttgtcag
     3421 gacaggtgtt attgccatcg aaagaggcaa tcggataata aaaatcacaa ataacgagga
     3481 gagtgattcg aatggctaaa atgtattatg acagcgactg cagtctcggt cttcttaagg
     3541 gcaagacggt agcagttatc ggatatggaa gccaggggca cgcgcatgcc cagaatttga
     3601 aagacagtgg gatcaatgta gtcgtagggc ttgccccggg atctgccagg aggaagcagg
     3661 ctgaggaaga cggccttacg gtcatggata ccgccgaagc ggcaaaggcc ggagacctca
     3721 taatgatcct tactcctgac cagacacagg cggagatcta ttccagggat atcgagccca
     3781 acctgaagga cggagacata cttgcgttcg cgcacgggtt caacatccat ttcaaccaga
     3841 tcaagcctcc ggaatatgtc gatgtcataa tgatcgcacc caaggggccc ggccacactg
     3901 tcaggagcca gtaccttgaa ggccgcggcg ttccggcgct tatagccgtc caccaggatg
     3961 cgtcggggaa agcaaaggac tatgcgctgg cttatgcagc aggaataggt gccggaaggg
     4021 caggcatcct ggagactacc ttcagggaag agacggagac cgacctgttc ggagagcagg
     4081 cggtactgtg cggcggagtc acggaactga tgaaggccgg attcgaaaca ctcgtggaag
     4141 caggttacca gccggagatc gcatattttg agtgcatcca tgaaatgaag ctgatcgtcg
     4201 atctcatcaa ccagggcgga ttctcataca tgaggtactc catcagcgat accgcggaat
     4261 acggtgacta tgtgaccggc aagaggataa ttaccgagga gaccaggaag gaaatgaaga
     4321 aggtcctcag cgagatacag agcggtgagt tcgcatcgaa atggatcacc gagaacaagg
     4381 ccggcggaag ggcgcagttc cttgctacca gggcgcggga atccgagcac cagcttgaga
     4441 aggtaggacg cgaactcagg aagatgatga gctggctgaa gaaataaaga agagaaaagg
     4501 cgtcgcggat gaacgccgga tacttatggt agagaaatat gatatgggat tagtatggta
     4561 ttaaggaggt tatcacatgt cagcaaggat caggatattc gatacgacac tgagagacgg
     4621 ggaacagact ccgggagtca gtctgaatat acaggagaag cttgagatcg cacggcagct
     4681 tgcaaagctt ggcgtggaca taatcgaagc gggctttgcc atttcgtcgc ccggcgattt
     4741 catggcggta aagacagttg cggaaaacgt aaagggaccc gtcatcgcaa gcctcagcag
     4801 ggccgtcgaa aaggacatag acagggcatg ggaagcactg cagaatgcgg aaaacccgcg
     4861 tatacacaca tttatagcca catcggacat ccatatgaaa tacaagctga agatgaccga
     4921 ggaagaggtg ctcgaacggg cagcagcaat ggtcagatat gccaagaaat attgcagcga
     4981 tgtggagttc tcggcagaag acgccagcag gaccaggacg gaattcctct acagggtgct
     5041 cgaagaagtc atcaaggcag gggctaccgt cgtgaacata ccggacacgg tcggctatgc
     5101 gatgcctgat gaattcggca gacttataaa aggcataaag gaaaatgtca gagggatcga
     5161 aaaagtagat ataagcgtac attgccacaa tgaccttggc ctggcggtgg cgaactcgct
     5221 gtcggctgtc atgaacggcg ccacccaggt ggagtgcacc atcaacggaa tgggcgaacg
     5281 tgccggaaat gcctcgatgg aagagataat aatgggcatc cagaccagga aggatttcta
     5341 cagcggaatg cagcacaaca ttgatacgag gcagatatac aggaccagca agcttgtgag
     5401 caaactgacg ggtgtgaacc tgcagccgca caaggccata gtcggcaaaa acgcattcgc
     5461 ccatgaatcc ggcatacacc agcacggagt tctggctgaa agatccacgt atgagataat
     5521 gactcccgaa tccattggcc tttccaaaaa caggatagtc ctggggaaac tctcggggag
     5581 gcatgcattt gaagagcgac ttaaggagat ggggtataac ctgccgcctg aagaggtaac
     5641 aaaggcattt gaaaaattca aggagcttgc tgacaagaag aagcatgtaa cggacaggga
     5701 tatagaagcc ctgattggcg agaaagtgtc ggaggtcgaa gaggtctaca agcttgacag
     5761 cttccagatc aacagcggta acagggtcat tgcgacggca accgtgagcc tgtttaaaaa
     5821 cggcgagctc atgacggaag cggcgaccgg cgacggaccg ataaacgcgg cattcaaagc
     5881 tatcgagaga tcagtggggt tcgaagtgca cctggaggat tacagcctca aaggcgttac
     5941 tgaaggcgcg gatgccctcg gcgaggccac tgtcagggta atgaaggaca acaggatata
     6001 tgtcggacgc ggcgtgagca cggacgttat agaagccagc gtcaaggcat atatcaatgc
     6061 gatcaacagg ataatacacg accatggcaa ggagacctcc ggcggaaggg agacaatatg
     6121 aaaaaggtag ttgtgcttga ttccacattg agagacggtg cccaggcgct gggcatctcg
     6181 tttacggtcg aagacaagct gaaaatcgtc aggcagcttg acaagcttgg cgttgcgtat
     6241 atagaagcgg gaaacccggg atccaatccc aaggaccttg agttctttga acgtggctca
     6301 aggctgaagc tgaaaaactc aaagctgata gcgttcggca gcacaagaag agtagggata
     6361 cccgtggatg aggatgcaaa tgtgagatcg ctgctgaaag ccggcaccga tgcggtggtc
     6421 atattcggca aatcttggga tttccaggtt accgagatcc tcaagaccac cttcgatgag
     6481 aacctgagga tgataaccga cactgtgagc tatttcagga agctgggcaa agaagtcgta
     6541 tatgatgctg aacacttttt cgacgggtac ttttccaatc ccgaatatgc gatggaaacg
     6601 ctgaaagctg ccgccgaagc aggtgcggac tgtctgtgcc tgtgtgacac caagggcgga
     6661 tgcctgccga tggatgtcta tgaaataacc aaaaaagtcg tggaacaatt tgacgtaccg
     6721 ataggcatcc atacacacaa cgatatgggt atggcggtgg ccagcaccat aatggcagtg
     6781 caggcgggag cgacacagat acaggggacg ataaacggat tcggcgaaag gtgcggaaat
     6841 gcgaacttgt gtacaataat cccgacatta cagcttaaga tggggtatga atgcataccc
     6901 agggaaagca tgcatgaaat cacgccggtg gccagggctg tcagcgagat agctaacgta
     6961 gtgcatgatg aaagggcccc atatgtcggg agatccgcat ttgcccacaa agcgggcatg
     7021 catgctgatg ccgttgtgaa aaacacgttc gcatatgaac tccttgaccc ggccgaagta
     7081 gggaaccgaa ggaccttcct catgtcggag gtagcaggca ggagtgcggt actgaacctg
     7141 atccagaagg tcgatccctc aatcacgaag gattcgccgg agacccggca gatactggac
     7201 aggctgaagg aaatggaaca ccagggatac cagtatgaag gtgctgaaag ctctttcgaa
     7261 ctcataatca gaaagatgct tggaaagtat aagccgttct tcgaactgaa agatttcaaa
     7321 gtgatagtca ccgaacctgc aaaacgcggg gaatggaatt catcggcgat gatcaaggtc
     7381 aaggtcgggg accaggagga gataaccgca gcggaaggca taggtcccgt caatgctctc
     7441 gacaacgcag tcagaaaagc gctgtcccgg ttctatccgg tcataaacag gatgaaactg
     7501 acggactaca aggtcagggt gctcgactcc gagtcggcta cggcagcaaa agtcagggtc
     7561 ctgatagagt ccactgacgg caacgaggtg tggaccacca tcggagtatc caccgacata
     7621 atagaagcaa gctggaaggc gcttgccgat tcgatcgaat acaagctcgg caacgggcat
     7681 acggcagaat aaagcaggcc ttacatgaaa ggcggcagtg atgactctca aaggaaagat
     7741 aataattgac ggtaaaatag caagagaagg cgcatcatat gacaatgaca gtgctcacag
     7801 cttcagggac aagcttgaga actgtcttgt cgatttgtgc gcagatcttg agatacccgt
     7861 tccgttatgg ctgaaaaaga atacgcggga acttgccgtg ttcagaaaaa cattcttctc
     7921 atccgaacag ttcattgaaa aagtgtggtt tgataaattc gagatagtac tgtcagccga
     7981 ggagtaagct ttctttttct ccagtctgtt ttgcaagccg gcataccggt cttttctttt
     8041 attgcatttt gtaaatactt gttgttataa tgtacatgaa gcagtttgga ttgtaattac
     8101 ttagcgtttc gaatgaggtg cgtaatgcat acatgcagaa atactgattt acgtccgact
     8161 ggcattgata ctgacagcat aagtgtttcc aatgaatgga taccgatcat tctctgctct
     8221 gccgtcaata ttgcattgct cacactcctg ctcagcacgt atacaaataa gcaactccaa
     8281 ctgagtttat tcggtttgac catagattat tacatattcg gcggttttgc cgtccaggcg
     8341 caggtcctcg tcaataccta cctttcaata aagacgatga agaaagggtt tatagcagta
     8401 gttttgctga atatactcgg catctgcttt gcgggcatcg gcagtgtcat catgtcagga
     8461 cagatggcag cattgcccgg ggtcgtgaca tatttcagtt ccacagtgat ctcgacagtg
     8521 ctctattatt acaaaaaagg ccttcgtgac aatatacaga ggcttaccga gcagagggac
     8581 gagataacat cgctgtaccg ggagataagc gcttccaaag agaaactgag ctagcagaat
     8641 gagcagctga agtggtacaa caaagtcatg aaggaaaatg agaagaaact tgagaggctg
     8701 gcttactacg atcctctcac cgggctcccg aaccgtaaga tgatcattca gaagctagat
     8761 atgctcatac attactccga acggtctcag gaaggcttcg cgctggtcta tatcgatatt
     8821 gacaatttca aggaaatcaa tgacctgatg gggcaccaca taggcgacat gctcctcaaa
     8881 gaggtaacga agcggtggaa agtaaggctg aacaagaaag atgttctcgg ccgcatggaa
     8941 ggcgatgagt ttgccatcat cataaggcgg gagctgagcg atgaagacct cctcagatat
     9001 gtggagtctt tcagggatgc gatttccgat gttttcagct atggcatgaa agagttaagg
     9061 atagaggcga gcttcggcat ctcagtctat ccgaaggacg ggcgcaccac tgaggacctt
     9121 ctgaaacatg ccggcatggc acaggcttat gtcaaggcgt caggcaaaaa aggcatagaa
     9181 tttttccggc atgaaatgca gaagaacatg atcgagcggg tccaactgga agacgggctg
     9241 aaatccgcga ttttgaaccg cgaactgttc gtggtcttcc agccgcaata ctcctgtgaa
     9301 acgcagaaga taagagggtt tgaggcattg gtcagatggc gctcgaaaaa gcttggcatg
     9361 gtcagcccgg ccacgttcat acctattgct gaagagacgg gcctgattat agatatcggc
     9421 gagtggatcc tgaggacggt catcaataag ttcataaagg tgaaagcggc caacagggac
     9481 tatatactga ccataaacat ttccgtcgtg cagataatac agccctcttt tgtttcgatg
     9541 gtaaagaaga tccttgatga aacggggttt gacggaagcc gcctggaatt cgagataacc
     9601 gagtctgtgt tcatttcata tcctgaaaag gtcatcaata cgctgaaaca gctaaaggag
     9661 atgggcatca gcatcgccct cgacgatttc ggcaccggct atgcatcgct gaagtatctc
     9721 cagatgctgc ccatagatac tttgaagata gacaggactt ttgtcagcgg gatcggggaa
     9781 atggaatccg gcaaccagat cgtcggctca ataatcgcgc tggcacacag ccttggcatc
     9841 cttgtcgtgg ccgaaggcgt cgagaacatg gaccagatga actatctgaa agagcagaaa
     9901 tgcgactgcg tgcagggctt tttgctcagt atgccgctgg aaggcgacca ggtagaccag
     9961 cttattgcaa cggcttagta atattgcctg gcgcaacgcc cgggcacttg ctgcaaagtg
    10021 atattaccaa atagttacga tttcaggaat ccttccgata tctctggttg caggataccg
    10081 gaaggatttt tcgtacacgg ggacggttct cctgtctcat agctgcagtc atcagcttcc
    10141 ggtcaaagtt aaacggcgca tgctttatat tatctatccg ttccgcatat aaataaactg
    10201 gtaatcgcac cttatcttat atttccttgg cccaaggggt gtttctgttg accaggttta
    10261 agttttttgc ggtttatttt tttattgtgc ttatgttgct gtctgccata caaataatca
    10321 tcgatgccgg ggaagagaag gcccggaatg ctgccgtact ggatgaaaaa agagaataca
    10381 cgatattcat tgaaatagaa gacaaaacgc tgtatctcct cgatggcaac acatgcgtca
    10441 aggagtacag gatcgcgtcc ggaaaatccg gctatccgtc gcctttgggc tgctggacca
    10501 ttatcgagaa gggcgactgg ggcgagggtt tcggaggaag atggctggga ctggatgtgc
    10561 catgggggaa atatggcata cacgggacca gggagagcgg atcgataggt accgccgcca
    10621 gccacggctg catccggatg ttcaatgatg atgtggcgga gttgtacagc ctggtggaga
    10681 taggcaccga ggtggttatc gtcaacggcc agttcgggcc gttcgggagg aaatttgatg
    10741 agataaaccc gggagacagg ggagcggatg ttctcgcaat acaaagaagg ctgaagcagc
    10801 ttggatacta taacggtccg ctggacggca tttatgagga cgacatgaaa aaggcggtcc
    10861 atcagttcca gcgcggcaca ggccttaagg cgcagaacac gatcgaccgg cagacatggc
    10921 tgaacatggg gtttcgggag tttgagtgaa tcgcatattt gttattataa tacgtgggtt
    10981 gttattattt gacaagtata ataactcact atatgataat aaaggtgatt gctatatgcg
    11041 taaaactatt gcttttttat tagcgcttat cactcttttc ttaacttcat gtcagaattc
    11101 gaataatacg gccgataatt gcaaagatga tccgaatgtt ataaaaagat atgaaaaaac
    11161 cgaacttggt
//
LOCUS       DAOSCC010000063        11148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3896_length_11148_cov_5.010367, whole genome shotgun sequence.
ACCESSION   DAOSCC010000063 DAOSCC010000000
VERSION     DAOSCC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3896_length_11148_cov_5.010367"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1079)
                     /locus_tag="PLG72_04925"
     CDS             complement(261..1079)
                     /locus_tag="PLG72_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOJ80175.1"
                     /translation="MGRRKKQVGSSGGASWLTTYSDLVTLLLCFFVLLFSMAVVDKQK
                     VTKVALSIRAAFLGKTGNEIFELKDGDTLISLTPYEKELIDMMDEEPGEEGEEADRTE
                     GEEETGEEDEPGENEDETGEEAGGIGTLKADIQGLIDAMGLNENIRVIDEGTRIILRM
                     DSVILFDTGKADIKPSAKPVIEKIGDILKTLDTEIQVQGHADDRPINTPEFPSNWELS
                     TKRATNVVKFLIDECGIDPKYLTATGNAEFRPIAPNDSEYNRQKNRRIDIAIYK"
     gene            complement(1094..1900)
                     /locus_tag="PLG72_04930"
     CDS             complement(1094..1900)
                     /locus_tag="PLG72_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOJ80176.1"
                     /translation="MDLGTILGLVLGVFCVVISIYIGEGEIRSYLHLPSAFITIGGGI
                     CSALINFRLKDFIDVFKVLKKVFTDKHSTASETIAMLVKLSEKTRREGLLSIEPELEQ
                     MEDPFIRQSLQLVVDGIESETIKDFMDTEIENMQARHSKYQAIFNMLGAIFPAWGMIG
                     TLLGLINLLLKLDDPSQIGPSMSVALVTTFYGSILSNFICIPFAGKLKMKSDEEVHQK
                     EMIAEAVISIQAGENPKMLEQKLKIFLTPEEREKGIRKNEEEATDSSVSA"
     gene            complement(2245..3531)
                     /locus_tag="PLG72_04935"
     CDS             complement(2245..3531)
                     /locus_tag="PLG72_04935"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HOJ80177.1"
                     /translation="METNMAARKLDIGRLREICIRKWDSTPDVFPVLYKRYTDSDHAA
                     NNVRIRKFIDDFAGLVRGFDGKPGEDHVRWGSCLKKLVYDCGVNVAGFDDPDMRLLLD
                     GGFCDATSDFIEQARTFDISLKLDDIFQALRNVWIMNCIQVFLGQRVRITPSVFAYSM
                     LYPYTDNFLDTGSISGTQKQDINSRLEKRLAGERVISETPLENRLFRLVEMIEGQYDR
                     KLFPMVYESLLGIHGAQVRSMRQDIASPLPFEEIKDISAEKGGCSVLADGCLVKGRLS
                     ENEATFIFCFGMLLQLVDDLQDAADDRKNGHKTLFTANGGGTSAKGITNKLINFTFRL
                     FDNDGIFAGSTAIRMKKVIKDSIILLVMAAAASNSSMYEKTYLKDLEEYSPVDFKTLL
                     DCRKRINREYAKLKIKLTVKPLEVQMAKAFAAGTLD"
     gene            complement(3546..4040)
                     /locus_tag="PLG72_04940"
     CDS             complement(3546..4040)
                     /locus_tag="PLG72_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ80178.1"
                     /translation="MRRLHRSKRNKVILGVCGGLAEYLNIDVVIVRIIAILALLMGWG
                     FIIYIIAALIMPEDKGYTPNEGDWGQGRSTAGYNNTYSDPGMGTGPDYDIGNDFFSDA
                     DSWDRPARYDSEKTKYIIGAILIGIGVIALGKQFLPALFRLEYMLPVLLIVIGGIILY
                     KGRR"
     gene            complement(4207..4932)
                     /locus_tag="PLG72_04945"
     CDS             complement(4207..4932)
                     /locus_tag="PLG72_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOJ80179.1"
                     /translation="MRYRMISCEVFARPAYRAAARSPHIVDIIFTRLRSHVDPHQLRN
                     EIQAYIDSSTEGYDAVLLGYGLCGNSTAGLKARNIPLVIPRAHDCCTIFLGSREAFLE
                     HFGQTPSAPWSSVCYYERLGGWYTDCAAGVTTSEQAGYMEELIRQYGEENARYIIEMM
                     TPKNDVGFLTFIDIEGFEDPGVRESFIMYAKESGKDTRFITGSTRLIDALVNGDWNDE
                     EFLIVPPGAEIIPVYDHKTIISC"
     gene            complement(5122..5529)
                     /locus_tag="PLG72_04950"
     CDS             complement(5122..5529)
                     /locus_tag="PLG72_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ80180.1"
                     /translation="MSEQVKEQPKREDRIRISEEVIATIAGIAASGNENVASMGGGFV
                     DGIAGMLGRKAPSKGIKVELRDNQVSIDMAVVMQYGCKIHEAARDMQKRVRDAVEDMT
                     GLEVVNVNVNVLGINISKDAGKVEVIDELPPRA"
     gene            complement(5710..7101)
                     /gene="asnS"
                     /locus_tag="PLG72_04955"
     CDS             complement(5710..7101)
                     /gene="asnS"
                     /locus_tag="PLG72_04955"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOJ80181.1"
                     /translation="MKIEIKQLFRDPAPYFGREIEVAGWIRTIRNSKTFGFIELNDGS
                     FLKNLQIVFEEGTISNYSEVAKLNQGTSIRACGTLVATPEAKQPFELKASAIDVEGAC
                     APEYPLQKKRHSFEFLRTIAHLRPRTNTFSAVFRLRSALSYAIHRFFQERGFIYVHTP
                     IITTSDCEGAGEMFRVTTMDPDNIPRDENGNVDYSQDFFGRQTSLTVSGQLAGEAYCM
                     AFGKIYTFGPTFRAENSNTTRHAAEFWMIEPEIAFADLEDDMQLAEDMMKYLINYCLE
                     NLPEEMEFFNSFIDNGLLERLNNIVGSDFGHVTYTEAISILEKNNSSFQYPVRWGSDL
                     QTEHERYLTETVFKKPVFVTDYPKDIKAFYMRLNDDGKTVAAMDLLVPGVGEIIGGSQ
                     REERYELLAKRIEELGMDPEDYRWYLDLRKYGGTKHAGFGLGFERAVMYLTGMPNIRD
                     VIPFPRTAKSAEF"
     gene            7306..7899
                     /locus_tag="PLG72_04960"
     CDS             7306..7899
                     /locus_tag="PLG72_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ80182.1"
                     /translation="MKILVVSDREEPYIWDYFDRERFRDVELVLSCGDLKAEYLSFLV
                     TMINAPLFYVPGNHNKHYLTNPPEGCESADDRLLVYNGVRILGLGGCMNYNYMEFQYT
                     EEQMQKRVRRLKRTIKKHKGFDILLTHSPAYGLGDGDDLAHTGFKCFVDLLDTYSPKY
                     FFHGHQHLNYGRRSRIMQYKNTTIVNAYGYYLIDCEF"
     gene            complement(7910..9790)
                     /locus_tag="PLG72_04965"
     CDS             complement(7910..9790)
                     /locus_tag="PLG72_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ80183.1"
                     /translation="MDRSGAENHYVNARKLGQKEYSRSASKGMTGYLPALEGILKNIE
                     IIYEIDLGVVEIPIKKIVGTYTNGRSRSFAVNFMPLLTPRTEFAAKWTALCEAHLNEG
                     IRDPIKVYEYLNWFYVVEGNKRVSVLKYFDAWSIQGRVMRLVPKKDESDDDISIYYEF
                     LDFYKKTRINTIWFTKRNSFNKLLGYLENFEPDQNLIGYDKYKYFTNSIYLPFRKIYL
                     ELGGQELPITTGDAFLEYITIYGMPKEIDEEKVRARLNSLIIELDQMSGNKPVDIQTM
                     PMETGEGLISTLTAFVMPRKTLKVAFVYARDTKTSSWTYAQEMGRIHVSKVFGDLIST
                     SYVDNVPETPEAYETLKKLALEGNDVVFVTSPAFINATLKAAMAFPGTKFLNCSETHT
                     YKHVNTYYGRIYEPRFLAGIAAGAVTKTNLLGYVGTLPVPGVINGINSFALGARLVNP
                     EARVKAVFLNRWDDAREAEAASESLIRQGCDVISHHDTLSDRSVTSEYGVYAAAISEE
                     GLVHEHIAAPVWNWGIFYEKMLHNLMKSSKKSFSPGQKVINFWWGMDSGIVDFFYSRR
                     LVPPETQKLLDFMIKMISNGLYHPFTGPIYSQDGTLMVRKGEVATREQIINMDWFVNI
                     VE"
     gene            complement(9876..>11148)
                     /locus_tag="PLG72_04970"
     CDS             complement(9876..>11148)
                     /locus_tag="PLG72_04970"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOJ80184.1"
                     /translation="KEVGVGFVLQLAGAIAAEMLKAFFADYFFPVMCLLVITFISSHY
                     RVYSELQEEIYGSPGIGLKQVAEKLILTGLVAGFAVSFLTVAAGITIETDTIRYLFYI
                     MCLLMLFDLRFVSFPYAAGLLAAASYIFDKLEVNVPSLLCLAAILQMLEAFLVFVSRK
                     SGYIPVYLHHDGEIAGAFLIRRFWIIPVVFLTYLLQPGTLLPEGMAGLPLPFVPRSLA
                     GAAGALGLDCMMALFCHSDISIATQPEKRSARSAAALFIYGSILMALALISRNVGWVG
                     CVGAVFCIAGREGISVWSLTEEKRRAPLFSAVRRGLRVLDVLPGSNAQLMGIMRGDII
                     LSINNMDIQTEEGIGEALKGFPVYTWIKVLRNDEEKLLEYRCYPDGYNTLGIITVPRE
                     KEVTYRIAWLENMSIIRNIVNRFRSNIDRQV"
BASE COUNT         2565 a         3097 c         2456 g         3030 t
ORIGIN      
        1 aggaaaaggg acacttctca tccatcgaag tcccgcttcc tgcagaggag tgtccatcgt
       61 aagctaaagg aaaagggaca cttctcatcc atcgaagtcc cgcttcctgc agaggagtgt
      121 ccatcgtaag ctaaaggaaa agggacactt ctcatccatc gaagtcccgc ttcctgcaga
      181 ggagtgtcca tcgtaagcta aaggaaaagg gacacttctc atccatcgaa gtcccgcttc
      241 ctgcagagga gtgtccctta tcatttataa atcgctatat cgatccttct gttcttctgc
      301 ctgttatatt cgctgtcgtt cggcgctatc ggcctgaatt cggcgtttcc tgttgcggtg
      361 agatatttcg ggtcaatacc gcattcatct atcagaaact taacgacatt cgtcgccctc
      421 ttggtcgaca gttcccagtt cgacggaaat tccggagtat ttatcggacg gtcgtcggcg
      481 tgaccctgca cctggatctc agtatcaagc gtcttgagta tatcgcctat cttctcaatg
      541 acaggtttcg cagatggttt tatgtccgcc ttgcctgtat cgaacaggat aaccgagtcc
      601 atcctcagga ttatcctggt gccctcatct atcaccctga tattttcatt cagtcccatt
      661 gcgtcaatca aaccctgtat gtcagccttc agtgttccaa tgcccccggc ctcctcaccg
      721 gtttcgtctt cgttctcacc gggttcatcc tcttccccgg tctcttcctc cccttcggtc
      781 cggtcagcct cttcgccttc ctctccgggc tcttcatcca tcatatctat cagttccttc
      841 tcgtacggag tgaggctgat cagagtatca ccatctttca attcgaatat ttcgttccct
      901 gtcttgccca aaaaggcggc acgtatagac aaagccactt ttgtaacttt ctgcttgtcg
      961 actaccgcca ttgaaaaaag cagcacgaag aagcaaagaa gcagtgtcac caaatcgctg
     1021 tatgttgtca gccatgacgc gccgcccgag cttcctacct gtttttttct tctgcccatc
     1081 cgtttcactc ctgttatgcg gatacagaac tatccgtcgc ttcttcttca ttcttcctga
     1141 tgcctttttc tctttcctcc ggtgtaagga agatcttcag tttctgctcc agcatcttgg
     1201 ggttctctcc ggcctggata gatatgaccg cttccgcgat catctctttc tggtggacct
     1261 cctcatcgct cttcatcttc agcttcccgg caaaagggat acagatgaag ttcgacagaa
     1321 tactgccgta aaatgtcgtt accagggcta ccgacatcga cggccctatc tgtgaagggt
     1381 catcaagctt cagaagcagg ttgatgagac ccagcagcgt accgatcatg ccccacgccg
     1441 ggaaaatggc gccaagcata ttgaagatgg cctggtattt gctgtgccgc gcttgcatgt
     1501 tctcgatctc ggtatccatg aaatccttta tggtttccga ttcaataccg tccacaacca
     1561 actggagcga ttgcctgata aaaggatcct ccatctgttc aagctccggc tcgatggaca
     1621 gcaatccttc cctcctggtc ttctccgaca gcttgaccag cattgcgatc gtttcacttg
     1681 ccgtggaatg cttgtctgta aaaacctttt tcaatacctt gaacacatcg ataaaatctt
     1741 tgagcctgaa gtttatcagt gcggaacata ttccgccgcc gatggtaatg aatgccgacg
     1801 gaagatgcag ataagacctt atttctcctt caccgatata aattgagatt actacacaga
     1861 aaacacctaa aaccaaacct aaaatcgtac ctaaatccat atcattcatc cctttgttgt
     1921 gaaaggtgat ttatccgctg ttcaaatatc atatcggaca aagaggcgat tttcattata
     1981 tcgattttac ttatacgcaa gaaaaaagaa cagattttat aaaaaacgag attacgaagc
     2041 cggatccctg cagtaaatca gaaaatatgt aaaagccggt gaatcgatga catgagtcca
     2101 ggcgagaaaa aagcgccggg aaaaatgccg atacttaggc tccggcgaga aaatgcaggc
     2161 aggaaagata ccagcgatat aattcgggtt agaaatatgc agtcaaagga acagagtatc
     2221 aggaactgat cacggatccc cgtgctaatc aagggtccct gcggcgaatg cctttgccat
     2281 ttggacttcc aacggtttga ccgtaagttt gatcttcagt ttcgcatatt ccctgtttat
     2341 ccttttccgg cagtccaaaa gggttttaaa atcaacaggc gagtattcct ccagatcttt
     2401 caggtatgtt ttttcataca tgctgctgtt gctcgcggcc gccgccatta caagcaatat
     2461 gatgctgtcc tttattacct ttttcatccg gatcgccgtg cttccggcaa atatcccgtc
     2521 attatcaaaa agcctgaacg taaagttgat aagcttgttt gtaatgccct ttgcggatgt
     2581 ccctccgccg ttggccgtaa aaagcgtttt atgcccgttc tttctgtcat ccgccgcgtc
     2641 ctgcaggtca tcgaccaatt gcaggagcat cccgaagcag aaaatgaaag ttgcctcgtt
     2701 ttccgacagg cggcctttca ccaggcagcc gtcagccagg acggaacacc ctcccttttc
     2761 ggcgcttatg tcctttatct cctcgaacgg caacggagac gcaatgtcct ggcgcatgct
     2821 tcttacctgg gcgccatgta tgccgagaag gctctcatat accatgggaa agagtttgcg
     2881 gtcatactgt ccttcgatca tctccacaag cctgaacagc ctgttttcaa gcggagtttc
     2941 agatatgacg cgttcgccgg caagcctttt ttcaagccgg ctgtttatat cctgcttctg
     3001 cgttccggat atgcttccgg tatcgaggaa gttgtcagta tagggatata acatgctgta
     3061 tgcgaaaaca gacggtgtga tccggaccct ttggcccagg aacacctgta tgcagttcat
     3121 tatccagaca tttctcaaag cctggaatat atcatcgagt ttcagggata tgtcgaatgt
     3181 gcgggcctgc tcgatgaaat cggatgttgc gtcgcaaaag ccgccatcaa gcagcagcct
     3241 catgtccgga tcatcgaaac ccgccacatt gacgccgcag tcatatacaa gctttttaag
     3301 acatgacccc catctgacgt ggtcctctcc gggcttgccg tcgaatcccc tgacaagccc
     3361 ggcgaaatca tcgataaact tcctgattcg aacattgttc gcagcatggt cgctgtcggt
     3421 ataccttttg tacagtacgg gaaaaacgtc cggagtgctg tcccactttc tgatgcagat
     3481 ctccctgagt ctgccgatat ccagttttct tgccgccatg tttgtttcca tcagatcccc
     3541 gccgtttatc ttctgccttt gtagagtatt attcctccga tcactataag gagcaccggc
     3601 agcatatact cgagccggaa cagggcaggc aggaactgtt tgccgagagc tatgaccccg
     3661 atgcctatca gtatggcacc aatgatatat tttgtctttt cggagtcata cctggcaggg
     3721 cggtcccaac tgtcagcatc gctgaaaaag tcattcccga tatcgtagtc cggacctgtc
     3781 cccatgccgg gatccgaata cgtattgtta tagcctgccg tgctgcggcc ctggccccag
     3841 tcgccttcat tgggcgtata gcccttgtcc tcaggcataa taagcgctgc tatgatgtag
     3901 atgatgaatc cccagcccat cagcaatgcc agtatagcaa tgatccttac gatcactaca
     3961 tctatgttca ggtactcggc caggcccccg cacactccca atattacctt gttccttttc
     4021 gaccgatgta atctcctcat atccattctc ctcttccata tgtgtctgca gtgtgtattc
     4081 ataagataat acgaagattt gtctgcaatg tctaatgatt tggagaattt tctgaaagtt
     4141 cctccgcatg gctgcccttg tatcttccgt ccggtgtata tccacaggcg gacctcctgg
     4201 tgccggtcag cagctgatta tggttttgtg gtcatagacc ggaatgattt ccgcacccgg
     4261 cggcacgatg aggaactctt cgtcgttcca gtccccgttg accagcgcgt ctatcagccg
     4321 tgtgcttccc gtgatgaacc gcgtgtcttt cccggactcc ttcgcataca ttatgaagga
     4381 ttccctgact ccaggatcct caaacccttc gatatctatg aacgtaagga agccgacatc
     4441 gtttttcgga gtcatcattt cgataatgta cctggcattt tcctctccgt actgccgtat
     4501 cagttcttcc atgtatcccg cctgctcaga tgtggtcacc ccggcggcac agtcggtata
     4561 ccatcctccc aggcgttcgt aatagcagac cgatgaccac ggggcgcttg gagtctggcc
     4621 gaaatgctca aggaaagctt cacggcttcc gaggaatatc gtgcagcagt catgggctct
     4681 tgggatcacc aacggtatat tccgtgcttt cagcccggcc gtactgttcc cgcaaaggcc
     4741 atatccgagc agtactgcat catagccttc ggtcgagctg tctatgtatg cctgtatctc
     4801 gttcctgagc tggtgcggat cgacatgcga cctcagccgg gtaaatatga tatccacgat
     4861 atgcggggat ctcgccgctg cgcggtacgc cggccttgcg aaaacctcgc atgaaatcat
     4921 cctgtatctc atatcctgac catccttaca gtaaaaacaa agcggcaaat cctgactcat
     4981 gccatccttt gccgctgtaa ccgcgtgttc atacgcgccg ttgtttcctg gcgcaaacgt
     5041 ttatttggat tgcttcccgg caggcatcag aaatctgtac tcccgggcca tccgtcattg
     5101 tgcatccgtt ccggtaacgg atcatgccct cggcggcagt tcatctatca cttcgacttt
     5161 tcccgcatcc ttgcttatgt tgatgccgag gacattaaca ttgacattca caacttcaag
     5221 gcctgtcata tcctcaactg catcccttac tctcttctgc atgtcacggg ctgcctcatg
     5281 gatcttgcag ccatactgca tcactacagc catatcgatg cttacctggt tgtccctcaa
     5341 ttccaccttg atacccttgc tgggagcttt ccttccgagc atgccggcga tgccgtctac
     5401 gaaacctcct cccatcgagg ccacattttc attgccggat gccgcaatac ctgcgatcgt
     5461 ggcgataact tcctccgaga tccttatcct gtcttccctc ttcggctgtt cctttacctg
     5521 ttcactcatc tcatacgcct cctttattac attttggcta taaatttcac acttacaata
     5581 tactttccca ctccggccat gtttcaagca aagttatatc tgccgaaaag ttcactgccg
     5641 atcgcacacc gctgccggta tcgcaccgat gaggaaatca cggcgttatg catcagttcc
     5701 ggcgtatggt cagaattcag ctgattttgc cgttcttggg aagggtatga catccctgat
     5761 attcggcatg cccgtaagat acatcactgc cctctcgaaa ccgagtccga acccggcatg
     5821 tttcgttccg ccgtacttcc gcaggtccag ataccacctg taatcttcag gatccatgcc
     5881 aagttcttct atcctctttg ccagcagttc gtatctttct tccctctggc tgccgcctat
     5941 gatctctccg acgccgggaa cgagcagatc cattgcggcc acggtctttc cgtcatcgtt
     6001 gagcctcatg tagaacgcct ttatatcttt cggatagtct gtcacgaata ccggcttttt
     6061 gaatacggtt tccgtcaggt accgttcatg ctccgtctgc aggtcggaac cccatcttac
     6121 cggatactgg aagctggaat tgttcttttc aaggatactg atcgcttcag tatacgtcac
     6181 atgtccaaaa tcggatccca ctatattatt cagcctttcc agcaggccat tgtcgatgaa
     6241 gctgttgaaa aactccatct cctcaggcag attttcaaga cagtaattta tcaggtattt
     6301 catcatatcc tctgcaagct gcatgtcgtc ttcgaggtct gcaaaggcga tctcgggttc
     6361 tatcatccag aactcggcag catgccttgt ggtattcgaa ttttccgccc tgaaagtggg
     6421 accgaacgta tatatcttcc cgaacgccat gcaataagct tctccggcaa gctggccgct
     6481 gacggtcagg ctcgtctgcc tgccgaaaaa gtcctgcgag taatcgacat ttccattctc
     6541 atccctcggt atgttgtcag ggtccatcgt ggtgactctg aacatttcgc ctgcgccctc
     6601 gcagtcgctc gtcgttatga tcggagtgtg gacatatatg aagcccctct cctggaagaa
     6661 acggtgtatt gcatatgaca gggcgctcct cagcctgaat accgcggaga acgtattcgt
     6721 cctcggtctc aggtgcgcga tcgtacgcag gaattcaaat gaatggcgct ttttctggag
     6781 cggatactcc ggagcacatg ccccttctac atcaatagcc gatgccttca gttcaaaagg
     6841 ctgttttgct tcaggcgtgg caacgagcgt cccgcaggcc ctgatcgatg tgccctggtt
     6901 aagttttgcg acttcgctgt aattgctgat ggtcccttct tcaaacacga tctgcagatt
     6961 tttaaggaac gaaccgtcgt tgagttcgat aaatccgaat gtttttgaat tccttatggt
     7021 cctgatccag cctgccactt ctatctccct gccgaaatac ggcgccggat ccctgaaaag
     7081 ctgctttatt tcgattttca tcggtatcct ccttttactg aagtgtttcg acatcaatac
     7141 gtatcttaat ataatatatc aaatatgcct tctaagtata tcctgaaatg gcagccggat
     7201 acaacaccgg attctgacat gtaccccgtg ccctttacat tattctttta aatcaagtat
     7261 aatacttaaa agatgcaaaa acaatatgag gaaaccgatg tgaaaatgaa gatactggtc
     7321 gtatcggacc gtgaagaacc atatatatgg gattacttcg acagggaacg cttcagggac
     7381 gtggagcttg tcctgtcatg cggcgatttg aaagcggaat atctttcctt cctggtgacg
     7441 atgataaatg cgcctttgtt ttacgtgccc ggcaaccaca ataagcacta tctcacaaat
     7501 ccgcccgaag gctgcgaatc tgcggacgac aggctgctgg tctacaatgg cgtcaggatc
     7561 ttaggccttg ggggatgtat gaactacaat tacatggagt tccagtatac cgaggagcag
     7621 atgcagaaaa gggtccggag gctgaagcgg acgataaaaa agcacaaagg tttcgatata
     7681 ctgctgaccc actcgcccgc atacggcctc ggcgacgggg acgacctcgc ccataccggt
     7741 ttcaaatgct ttgtggatct tcttgacaca tattccccga aatacttctt ccacggccac
     7801 cagcacctga actacggccg ccgttcaagg ataatgcagt ataagaacac cacgatagta
     7861 aatgcatacg gctattacct gatcgattgc gaattctgac tgcaattcct cactccacta
     7921 tattcacgaa ccagtccata tttatgatct gttcgcgggt tgccacttcg cccttccgga
     7981 ccatgagggt gccgtcctgg ctgtatatcg gcccggtaaa cggatggtag agcccgttgc
     8041 tgatcatctt tatcatgaag tccaggagtt tctgggtctc cggcggtaca agccttcttg
     8101 aatagaagaa gtccacgatc cccgaatcca taccccacca gaaattgatc accttctggc
     8161 cgggactgaa ggatttcttc gaactcttca tgaggttgtg cagcattttc tcatagaata
     8221 ttccccagtt ccagacaggg gctgcgatat gttcatggac caggccctcc tcgctgatgg
     8281 cagccgcgta tacgccgtat tcgcttgtca ctgacctgtc ggaaagcgta tcatgatgcg
     8341 aaatcacatc gcacccctgg cggatcagcg actcgctggc cgcctccgct tcacgggcat
     8401 cgtcccatcg gttcaggaaa accgctttga ccctggcttc cggatttaca agcctggctc
     8461 caagggcaaa ggagtttatg ccgtttataa ctcccggcac aggcaatgtc ccgacatatc
     8521 caagcaggtt cgttttcgta accgctccgg cagcgatccc ggcaaggaac ctcggttcat
     8581 atatcctgcc gtaataggtg ttcacgtgct tgtatgtatg ggtctcggaa cagttgagga
     8641 atttcgtccc gggaaatgcc attgccgcct tcagcgttgc gttgataaag gcagggctcg
     8701 tcacgaacac gacatcgttg ccttcaagtg ccagtttctt caatgtttcg tatgcctccg
     8761 gcgtctcagg cacattgtcc acataagagg tgcttatcag gtcgccgaag accttgctca
     8821 catggatcct tcccatctcc tgggcataag tccagcttga ggtctttgta tccctggcat
     8881 atacgaatgc gaccttcagc gtcttcctcg gcatcacgaa cgctgtcagc gttgaaatca
     8941 agccttctcc tgtctccatg ggcatggtct ggatgtccac aggtttgttg ccgctcatct
     9001 ggtccagttc aatgatcagg ctgttcagcc ttgcacgcac cttctcctcg tctatttcct
     9061 tcggcatgcc gtatatggtt atatattcga ggaacgcatc gcccgtggtg atcgggagtt
     9121 cctgccctcc aagctcgagg tatatcttcc tgaaaggcag gtatatggag ttggtgaaat
     9181 acttgtattt atcatacccg atcaggttct ggtccggttc gaaattctcc aggtaaccga
     9241 gcagtttgtt gaatgaattc cttttggtga accagatcgt atttatcctg gttttcttgt
     9301 aaaaatcaag aaactcgtag tatatcgata tgtcgtcgtc gctctcgtcc ttcttgggaa
     9361 cgagcctcat gaccctgccc tggatggacc aggcatcgaa gtacttcagc acgctgaccc
     9421 ttttattgcc ttcgaccaca taaaaccagt tcaggtactc atacaccttg atcggatccc
     9481 tgatgccctc attcagatgc gcttcacaca gagcggtcca cttggccgca aactccgtcc
     9541 ttggtgtcag cagcggcatg aaattgacgg cgaacgacct gctcctgcca tttgtatagg
     9601 tacctacgat ttttttgatc gggatctcca caacaccaag gtcgatctcg tatattatct
     9661 ctatattctt aaggatgccc tccagcgccg gaagatatcc cgtcataccc ttggaagcac
     9721 ttcgggagta ttccttctgg cccagttttc gggcattcac ataatggttt tcagctccgg
     9781 atctgtccat caccctcacc ctttcgttac gtatactgcg gtatctgcag tgatatttta
     9841 cctgcatgca tgaaatactc aagattcaat atcacttaaa cctgcctgtc gatattgctc
     9901 ctgaaacggt tgactatgtt cctgattatg ctcatattct ccagccatgc aattctgtac
     9961 gtcacttcct tctctctcgg cacggttatg atgccaagcg tgttgtaccc atccgggtag
    10021 cacctgtatt ccagcagttt ttcctcatcg ttcctgagca cctttatcca tgtgtaaacg
    10081 ggaaatcctt tcaatgcctc gccaatgccc tcctcggtct ggatgtccat attgttgatg
    10141 ctcagtatta tgtcacctct cataatgccc attaattgcg cgttgctccc cggaagcaca
    10201 tccaggaccc tcagtccgcg cctgacggcg ctgaacagcg gcgctcttct cttctcctcc
    10261 gttaacgacc agaccgatat gccctcacgc ccggcaatac agaaaacggc gccgacacac
    10321 ccgacccatc cgacatttct tgagatcagc gccagggcca tcagtatgct tccgtatatg
    10381 aaaagggccg ccgcacttct tgcggacctt ttctcaggct gagtcgctat cgaaatatcc
    10441 gaatggcaaa acagcgccat catgcaatca agccccagcg ctccggcagc tccggccagg
    10501 gacctgggta cgaacggcag aggcagtcct gccattccct cgggcagcag tgttccgggc
    10561 tgcagcaggt atgtcaggaa aacgaccggt atgatccaga atcttctaat caggaaagcg
    10621 cctgctattt caccgtcatg atgcaaatac acaggtatgt atccgctctt tctgctgaca
    10681 aagactaaga aagcctccag catctgcagt atggctgcca ggcacaacag cgaaggtacg
    10741 ttcacttcca gcttatcgaa aatatagctt gccgcagcaa gcaggcctgc cgcatacggg
    10801 aaactcacga atctcaggtc gaacagcatc aaaaggcaca taatgtaaaa cagatacctt
    10861 atagtatctg tttcgatcgt tatccctgct gccacggtaa ggaaactgac agcaaagccc
    10921 gcaaccagtc cggtcagtat cagtttttcg gcaacctgtt tcaggccaat tccgggactg
    10981 ccgtatattt cttcctgcag ttccgaatac acacggtaat gtgaacttat gaaggttatt
    11041 accaacaggc acataaccgg gaaaaagtaa tctgcaaaaa atgctttaag catctctgct
    11101 gctatagcac ctgccaactg cagcacaaaa ccaactccta cttccttt
//
LOCUS       DAOSCC010000064        11094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_3917_length_11094_cov_4.796540, whole genome shotgun sequence.
ACCESSION   DAOSCC010000064 DAOSCC010000000
VERSION     DAOSCC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_11094_cov_4.796540"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLG72_04975"
     CDS             complement(<1..302)
                     /locus_tag="PLG72_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase CgtA"
                     /protein_id="HOJ80185.1"
                     /translation="MFIDRAKIYIKAGDGGNGIVSFRREKYVPAGGPDGGDGGKGGDI
                     VFEVDEGLRTLIDFRYRRKYIAGSGDNGGPAKRTGKSGEDLVIRVPPGTVIRDAETG"
     gene            complement(362..640)
                     /gene="rpmA"
                     /locus_tag="PLG72_04980"
     CDS             complement(362..640)
                     /gene="rpmA"
                     /locus_tag="PLG72_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ80186.1"
                     /translation="MIRINLQLFAHKKGVGSSRNGRDSAAQRLGVKRGDGQYVLAGNI
                     IVRQRGTKIHPGANVGIGKDDTLFALKSGKVKFERLGKDKKQVSIVAM"
     gene            complement(633..977)
                     /locus_tag="PLG72_04985"
     CDS             complement(633..977)
                     /locus_tag="PLG72_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOJ80187.1"
                     /translation="MINVVITRDQTGPIRSIEISGHSGYAEAGSDIICSAAAAIGYTA
                     AGGLESLAGLRGCYKTDDGYMLIRLPEQMDEKQRSIASIIMETAAIGFKQMEFSYAEY
                     VSVKDEEVRSDD"
     gene            complement(981..1292)
                     /gene="rplU"
                     /locus_tag="PLG72_04990"
     CDS             complement(981..1292)
                     /gene="rplU"
                     /locus_tag="PLG72_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ80188.1"
                     /translation="MYAIIETGGKQYKVQEGDVLFIEKIEAEDGATVTFDKVLAVSSE
                     GSMTFGKPFVANASVTAKVLGQGRAKKIIVFKYKPKKGYRKKQGHRQPFTKVQIEKIS
                     V"
     gene            complement(1573..2868)
                     /locus_tag="PLG72_04995"
     CDS             complement(1573..2868)
                     /locus_tag="PLG72_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOJ80189.1"
                     /translation="MKKQLIADIGQDENRVALLEDGELAEIYIERPDIDRLVGNIYRG
                     KVCSVLPGMQAAFVDIGYEKNAFLYAGDIVARKEYSEDDDDHPHDVKNLNIADLVSAG
                     QELTVQVIKEPMGSKGPRVTTNITLPGRHLVLLPDADYTGVSRRIEDEEERAKLKKIA
                     EKLKPEGMGLIVRTASEGMEEEDFTDDLDFLLKLWENIKKKERSGPVPRCIHKDLSLV
                     YRMVRDLFTRDIDKFIINDRNHYEKILELVDIVSPDLKRRVEYFARDVDIFEYYQLDS
                     KLARALSRKVWLRCGGYLVIDRTEALTVIDVNTGKYVGRNNLEDTVLKTNMEAADEIA
                     RQLRLRDIGGIIIIDFIDMHDKEHQHMVLERLKQALKKDRTRTIVVGMTGLGLIEMTR
                     KKVRQDLSSIMQMECPYCEGTGRLLVSDVNGKVQKPGDR"
     gene            complement(3013..3747)
                     /locus_tag="PLG72_05000"
     CDS             complement(3013..3747)
                     /locus_tag="PLG72_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOJ80190.1"
                     /translation="MRIRFVRGESVKFLSHLDLMKVFERAVRRSGLPVSYSKGFNPHP
                     QMVLGLPLSVGMTSECEHADFQLDAEIEPAEFIERLNGSLPDGIRVTAAAFNNSRKNI
                     MATVRRADYELEVFTDMALAYEEASRGLAGMLKRESIKVGKETRGKRGETTVKETEIR
                     PMIFDAAIEELKTVPQGYEAFKSAFLVKAGLKAGSEANLSPGLFLKALAEQWGVPAAA
                     VRMHRKALYIDTGSGLTDPLDRSSLS"
     gene            complement(4066..5898)
                     /locus_tag="PLG72_05005"
                     /pseudo
     CDS             complement(4066..5898)
                     /locus_tag="PLG72_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
     gene            complement(6145..6636)
                     /locus_tag="PLG72_05010"
     CDS             complement(6145..6636)
                     /locus_tag="PLG72_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOJ80191.1"
                     /translation="MYIRKAESNDLPFIMKIYEKARLFMKNNGNPAQWWESGYPPEEI
                     VRKDIDDRNLYVCVENNNIVGVFAFIIGDEPTYRKIEHGAWHSSKQYGTIHRVASDCS
                     TRGIAKSCFDFCSQKSKYLRIDTHRDNKPMLAAIRNYGFRECGIIHVRDGSERIAFDY
                     IVK"
     gene            complement(6858..9014)
                     /locus_tag="PLG72_05015"
     CDS             complement(6858..9014)
                     /locus_tag="PLG72_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOJ80192.1"
                     /translation="MIWKAKWIAPAKPMGDVCPLFYRNFHIAGPVKKAILHITAVGVY
                     EASINGQRAGDFILAPGWTSYQKRHQYQDYDVTELLTEKNELCVLVGKGWYRSPIPGW
                     ITDSYEQRSLLPAAVIAQLDIETESGNKIQIVTDESWQVRESRIRFSEIYDGEHADFS
                     MPEAPLLPVRAVQFGMEQLIPQEGEIVRELDRIRPKRIFRTPKGEVVVDFGQEVTGYV
                     RFNVRAMKGDIVEISHAEVLDRDGNFYTENYRNAKAKLRYICCEGEQTYQPHLTFYGF
                     RYIRLDKWPGNALPENFEAVLVSSDMKRTGTLNCGIPKLNKLFENIVWSQRDNFLDVP
                     TDCPQRDERLGWTGDIAAFIKTACFNFDVEKFFRKWLLDLKADQLPDGSVPHVIPDVQ
                     VGSGSAAWGDASVLVPWQLYLFYGDEHLLSEQYESMEKYLRFIRESSADEYLWTGGKH
                     FGDWLGLDAPQGSYKGRSRDDFIASAYYAYDVCLMAKIARILGKDASKYEGLYDRIVQ
                     KFRKTFPDYLTQTENVLALVFGLTEDPERTAKELAQRVLDAGKRLETGFVGTQLLLFA
                     LSRNGYTELAYDLLLREVFPSWLYQVDHGATTVWEHWDGIMPNGEFWSPDMNSFNHYA
                     YGSVMGWVYEEAAGIHTIEESPGFRKIRIEPKPDRRLGWLEASLQTRRGLLDVSWRYE
                     DDSIRYEIQTPSDTDFYMDGRKYSLKPGRYVFFGSI"
     gene            complement(9714..9887)
                     /locus_tag="PLG72_05020"
     CDS             complement(9714..9887)
                     /locus_tag="PLG72_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80193.1"
                     /translation="MIIRNIGGEWEEEEGKGISVRKGVFACHPISKIYKRLKNGDQDD
                     IEIFYKNCKARMA"
     gene            complement(9884..10129)
                     /locus_tag="PLG72_05025"
     CDS             complement(9884..10129)
                     /locus_tag="PLG72_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80194.1"
                     /translation="MKSRGKIIIAVISIAIIGVLTYSWFNVRGDEIKGVSQISSDCTV
                     TIKVGDEDEKEYILTGDQIEMLRTLILESTFTKILVS"
     gene            10586..>11094
                     /locus_tag="PLG72_05030"
     CDS             10586..>11094
                     /locus_tag="PLG72_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80195.1"
                     /translation="MDEFRKPYEYMEEKGISGFLLVYFLMLLAQETLLGVITLFNGYD
                     LLSGNRVLGTIIMGISVLYILFSLFSAIVLKKLKKFAVRVSKVFLVFRAVYLIPYLIF
                     NTISQIRDIPYNIDHEKYSNMLGSIIVSSVVSFLLIVAFSAGWYMYLNRSKKVRELFP
                     VGIDGSKAPV"
BASE COUNT         2463 a         3138 c         2339 g         3154 t
ORIGIN      
        1 ccagtctccg catctctgat aacggtcccg ggcggcaccc tgatcacaag gtcctcgccg
       61 cttttgccgg tacgtttcgc agggccgccg ttatcaccgg atccggcaat atacttgcgc
      121 ctgtatctga agtcaatgag cgtccttaag ccttcatcca cctcaaatac gatgtcgccg
      181 ccctttccgc cgtctccgcc gtcagggccg ccggccggga catacttctc cctccggaac
      241 gaaactatac cgttcccgcc gtccccggct tttatatata ttttcgcgcg atcgataaac
      301 ataactttct cccatacgca aaaaagtccc gaccagttcg ccgggactta ttttttgcca
      361 actacatcgc tactatactt acctgtttct tgtccttgcc cagtctttcg aacttgacct
      421 ttccggattt cagggcaaag agcgtgtcgt ccttacctat tccgacattg gcgcccggat
      481 ggatcttcgt gcccctctgt cttactatga tattgccggc cagaacatac tgcccgtcgc
      541 ctctcttgac gcccagcctc tgagccgcac tgtcacggcc gttcctcgaa ctgccgacac
      601 ctttcttgtg agcaaacaac tgcagattga ttctaatcat cggatcgcac ctcctcatct
      661 tttaccgata cgtactctgc atacgagaat tccatctgtt tgaaacctat tgccgcggtt
      721 tccattatga tcgaagcgat cgatctttgc ttttcatcca tttgctccgg caacctgatc
      781 aacatgtatc cgtcgtctgt tttgtaacat cccctgagac ccgccaggct ttcaagccct
      841 ccggctgctg tgtagcctat cgccgccgcc gccgaacata tgatgtccga tcccgcctcg
      901 gcatagcctg aatgtccgct gatctcgatg cttctgatag gtcccgtttg atctcttgtt
      961 ataactacat ttatcatttc tcagacactg atcttttcga tctgtacttt tgtgaagggc
     1021 tgcctgtgtc cctgtttctt cctgtagccc ttcttcggct tatacttgaa gacgattatc
     1081 ttctttgccc tgccctggcc cagtaccttt gccgtaacac tggcatttgc cacgaacggc
     1141 ttgccgaatg tcatgcttcc ttcgctggat acagccagga ccttgtcgaa tgtgactgtt
     1201 gcaccgtctt cagcttcgat cttctcgatg aacagtacat cgccttcctg taccttatac
     1261 tgttttccgc ctgtctcaat tattgcgtac acgaatcata cctcctgcaa acagactcgc
     1321 cggacccccc tgaggcagcc gtcacttgcc ggccgtacat tccggatgac ttgccgtaca
     1381 tcccggaaac ggacatccgc ctgccttttg acagcatttt aaagccttgg ccgagcggtt
     1441 accgaagtat tttaacacat actccgacta attgtcaacg tctttgattt tgtctctgcc
     1501 tatctgtcca gggcagcatg tgcccttctc cggctcatca cttcgcccgc atcgtccggt
     1561 cttcggtttc atttacctgt cgcccggttt ctgcactttc ccgttcacat cggatacgag
     1621 cagcctgccg gtaccctcgc agtaagggca ctccatctgc attatgctgg acaggtcctg
     1681 ccttaccttc ttcctggtca tctcgatcag tccgaggccg gtcatgccga ccactatcgt
     1741 cctggtcctg tccttcttca gggcctgctt cagcctttca agcaccatat gctggtgctc
     1801 tttgtcgtgc atgtcgatga aatctattat tatgatgcct ccgatatccc gcagcctcaa
     1861 ctgcctcgct atttcgtccg ccgcttccat attggtcttc agcaccgtat cttcgagatt
     1921 gttcctgccg acatacttgc cagtgtttac gtcgataacg gtaagggctt ccgtcctgtc
     1981 gatcaccagg taacccccgc acctgagcca aaccttccgc gacagcgcac gggcaagctt
     2041 tgagtcaagc tggtaatatt caaaaatatc gacgtccctg gcaaaatact ccaccctgcg
     2101 cttcagatca ggggagacga tatcgaccag ttccagtatt ttctcgtagt ggttcctgtc
     2161 atttatgatg aacttgtcga tgtccctggt aaacaggtcc ctcaccatcc tgtaaaccag
     2221 gctcaggtct ttatgtatgc agcgtggtac tgggccgctt ctttcctttt tctttatgtt
     2281 ttcccacagc ttcagcagga agtccaggtc gtccgtgaaa tcttcctctt ccattccctc
     2341 ggaggcagtc ctgacgatca gccccatgcc ctcaggcttt agcttttccg cgatcttctt
     2401 aagcttcgct ctttcttctt cgtcctcgat cctcctggac actcccgtgt aatcggcatc
     2461 gggcagcagt accaggtgcc ttccgggcaa agtgatgttc gtggtgaccc tgggcccttt
     2521 ggaacccatt ggttccttta tgacctggac cgtcagttcc tggccggcgc tcaccaggtc
     2581 cgctatatta aggttcttta cgtcgtgagg atgatcatcg tcgtcttcgc tgtattcctt
     2641 ccttgctacg atatcgccgg catacaggaa ggcattcttt tcatacccga tatccacgaa
     2701 ggctgcctgc atccccggca gcacactgca gaccttgccc ctgtatatat tgccgaccag
     2761 cctgtcgata tcgggcctct caatatatat ttccgccagt tcgccatcct caagcagcgc
     2821 caccctgttt tcatcctgac cgatatctgc gatcaattgc tttttcaaat caacacctct
     2881 ttatttccgg gaaagagaag catgtctcat tatattccca tatatcgggc aaggaaacat
     2941 ccaaggcgat atctttcctt acatgtttcc catatcaggg aacgagacat cctgactctc
     3001 tgtttcaaaa cttcatgaaa gagaagacct gtccaatggg tccgtcaaac cgctgcctgt
     3061 atcgatgtac agagctttac ggtgcatcct tacagccgct gcgggaactc cccactgctc
     3121 agccagggcc ttcaggaaca gcccggggct gaggttcgct tcgctgcccg ccttaagtcc
     3181 ggcttttacc aggaaggcag atttaaacgc ttcgtaaccc tgcggcactg tcttgagttc
     3241 ttcgattgcc gcatcgaata tcatcggtct tatctcggtt tcctttacag tggtttcccc
     3301 gcgctttccc ctggtctcct tgccgacctt gatgctttca cgcttcagca ttcctgcaag
     3361 acccctggac gcttcttcgt atgccagcgc catgtcagtg aacacttcaa gctcataatc
     3421 tgcacgccgg acagttgcca tgatattctt tctcgagttg ttgaacgccg ccgctgtcac
     3481 cctgatcccg tccggcagcg aaccgttaag cctttcgatg aattccgcag gttcgatctc
     3541 cgcatccagt tggaaatccg catgctcaca ttcgctggtc atgccgacgc tcaacggcag
     3601 acccagcacc atctgcggat gcgggttgaa gccttttgag tatgagaccg gaaggccgga
     3661 acgccttacg gccctttcga atactttcat caggtcaagg tgcgacagga atttgaccga
     3721 ttcgcccctt acaaacctga tcctcatgct gcatgcaggc ctgccgtttg ccgtgccctg
     3781 tctgccgtcc gcggcgtggc ggcgttcttc atcctgtgca gttgcttcgc cggtgttccc
     3841 atgctggtcc attttgatat tggcatcttc ctgttgtgca gtatcaacaa gtgcatctgc
     3901 ctgccgcact ttttcgattt gctcatctct gcactgtggc gcatcgtttt ggatatcagc
     3961 cttcggtgcg ctttcaccat aagcattcgc ccactctgac ggatcaccag cgccacgatc
     4021 gcctgatctg cccttcccgg acttcttgcc ggcagtgtag ttagtacaga tgccctggtc
     4081 aaatatcgaa gctccgcatc ccgcacagga tgtcatgcag ttaggcgtga cttcaccttt
     4141 ttcagcccgt tttttctcac gcaggaggtg cttcttcgtg accccggcat ctatgtggtc
     4201 ccacgggaat acttcatcct cgctcctttt cctgttcgcg tagaatgccg ggtcgatgcc
     4261 gcagtcctcg aaagcctgca tccacgcatc gaacctgaag tggtcgcccc agctgtcaaa
     4321 gcggcatccc agctcccatg ccctgagcag aacgctccca agacgcctgt cgcccctggc
     4381 gaataccgct tcaagcatgc tcagttccgg atcatgccag ttatatttca ggtgcctgct
     4441 tcttatttta tctttcagga attcctgctt gtccctgagc gtttcgatcg tgtcctgggc
     4501 ttcccactgg aacggcgtga aaggcttggg cacaaaggac gacgtgctta tggtgacctc
     4561 aaggcctctt gccctctgtt ccttcgggat cctgaaatac tcgtccacca ccatcgtgcc
     4621 gagctttgcg attccctcca cgtcctccaa tgtctcaaaa ggcaggccga tcatgaagta
     4681 cagcttgacc ccgttccatc cggccctgaa ggccattccc acggaattca gcaggtcctc
     4741 ctcggtgacg cctttgttga tgatgtccct gagcctttgc gtgcctgcct cgggagcaaa
     4801 tgtaaggccg ctcttcctta ccttctgcac cttctccagg agatcgagtg aaaacgaatc
     4861 tatacgtaag gacgggagcg acaggttcac catcttcggc tccatttctt cgatcagtcc
     4921 cgatgtaagt tccggcagcc ccgagtaatc gctcgtactc agcgatgtca gcgatatctc
     4981 cccgtagccc gagctttcct ccagcttcct tgccagttcc atcagccgct caggggtcct
     5041 ctcccttacc ggcctgtaga tgtatcccgc ctggcagaac ctgcagcccc tagtgcagcc
     5101 ccggaacagt tcgagcatga tcctgttgtg gactacgtct atgtacggga ctatcacctt
     5161 ttcaggaaag tatacgttgt ccaggtcgct tatgatcctt ttccttatct ttgcaggata
     5221 tttatcattt atgggttcaa cagccttcag agtaccgtcg ccgttatatt caggctcata
     5281 aaaaccgggc acatatacgc cttcgatgcc ggcgatcctg tccaggaagt cctctcttcc
     5341 tgcattatct ttcttccact gcttgtacac atccaatact tcatttatga tctcttcgcc
     5401 ttcgccaagc atgaagaaat ccacaaaatc ggccaatggc tcggcattgt atgcgcaagg
     5461 ccctcccgca catacaaatg gcatgccttt caccctgtcg gtccgcttga gcggtatgcc
     5521 ggcaaggtcc agcatgttga gtatgttggt atagctcatc tcgtactgga gagtgaagcc
     5581 gactatatcg aattcgccga ccgggcttcg tgtctcaagc gagaaaagcg gtatcccgtt
     5641 ttcccgcatc cttgcttcca tgtcggtcca cggcgcaaat accctttcac agtacgtatc
     5701 gtcccgctcg ttgagcaggc cgtacagtat cctcaaacca aggtgggaca tcccaacctc
     5761 gtatacatcc ggaaaacaaa aggcaaacct gatatctgcc ttcgaaggat ccttaacaac
     5821 gctgttcagt tcgccgccgg tatatcttgc cggtttctcc acgctttgca atatgtcgtc
     5881 ccttataact actgccacaa aaacctcctg gaatcctgct ccatattgta ttttgcatcc
     5941 gcgaaaatct accttacaat aatataccca aacagaaacc aattagcaac agaagtaatg
     6001 tcagcaagta tgatagcata catgcattaa agaacaggac attggaacag tgttgccgga
     6061 taatttacag atacagctga tttctgattt ttgggttgaa cagattttaa tgtgtgttca
     6121 aaacggcgat cctttactct taactcattt tactatgtag tcgaacgcta ttctttcgct
     6181 gccgtcacgt acgtgaatta tcccacattc ccggaagcca taatttctta tagctgccag
     6241 catgggcttg ttgtccctgt gagtatcgat tctcaggtac ttggattttt ggctgcagaa
     6301 gtcgaagcaa cttttggcaa tcccccttgt gctgcagtcc gatgcgaccc ggtgaatcgt
     6361 cccgtattgc tttgagctgt gccaggctcc atgttcaatt ttccgataag tcggctcatc
     6421 tcctataata aatgcaaata cgccgacaat attgttattc tccacgcaaa catataaatt
     6481 cctgtcatca atatcttttc tgacgatttc ttccggcgga tatcccgatt cccaccactg
     6541 tgcaggattc ccgttgttct tcatgaacag cctggctttt tcatatattt tcattataaa
     6601 cggcaaatca ttgctttctg cttttctgat atacattttt cttcatcctt tcgtgataca
     6661 gctaaagtct attaggtatt ccagccggaa gcacaacaat gcaatgtata tcatatcgcg
     6721 tgattcgctt aaagaaaaga accgattctg acggctgata acctgatgcc cgccagcctg
     6781 aataataaaa acggcagacg ccccaattag gtcgtccgcc aacgatactt tattacttgc
     6841 aaattaacac aatgctctta aatactgcca aagaagacat aacgtccggg cttgaggcta
     6901 tattttctgc catccatata aaagtcagtg tctgatggtg tctgtatctc ataacgtatc
     6961 gagtcgtcct catatctcca gctgacatcc agcaagccgc gccttgtctg aagactggct
     7021 tccagccagc cgagacggcg gtcgggtttc ggctcgatcc ttatttttct gaacccgggg
     7081 ctttcctcaa tggtatggat accggcggct tcctcatata cccagcccat cacggatcca
     7141 taggcataat ggttgaaaga attcatatcc gggctccaga attcgccgtt gggcatgata
     7201 ccgtcccagt gttcccagac cgtcgtcgct ccatggtcga cctggtaaag ccacgacgga
     7261 aatacctccc gcagcagcag gtcataggca agctccgtat atccgttccg gcttagcgca
     7321 aaaagcagca gctgtgttcc gacaaaaccg gtctcaaggc gcttcccggc gtccaggacc
     7381 ctttgtgcaa gctccttcgc cgtacgctcc ggatcttccg tcaagccgaa tacgagcgcc
     7441 aatacatttt ccgtctgtgt caggtagtcc gggaatgttt tcctgaactt ttggacaatg
     7501 cggtcataga ggccctcata tttcgaggcg tctttcccga ggatgcgtgc gatctttgcc
     7561 atcaggcaca catcatatgc atagtaagcc gatgcgatga aatcgtcccg tgaacgtccc
     7621 ttatagcttc cctggggcgc gtcgagtccg agccagtcac cgaaatgttt tccgcctgtc
     7681 cagaggtact catcagcaga agattcgcgt ataaagcgga gatatttttc catactttcg
     7741 tattgctcac tcagaagatg ttcatctcca taaaacaggt ataattgcca cggaaccagt
     7801 accgacgcat cgccccacgc tgcgctgccg ctgccgacct gaacatcggg gatcacatgc
     7861 gggaccgatc cgtccggaag ctgatcagcc ttcaggtcca ggagccactt gcggaaaaac
     7921 ttctccacat cgaaattgaa gcatgccgtc ttgataaacg ctgcaatgtc acctgtccat
     7981 cccaggcgtt cgtcccgctg cgggcaatcc gtaggtacgt caagaaaatt gtcgcgttgg
     8041 ctccatacaa tgttctcaaa gagcttattg agtttcggga taccgcagtt aagcgttccc
     8101 gtccgcttca tgtccgaaga aacaaggact gcttcaaagt tttccggcag cgcgttcccg
     8161 ggccatttgt caagccggat ataacggaat ccgtaaaatg tcaggtgagg ctggtaggtc
     8221 tgctcccctt cacagcatat ataacgaagc tttgcttttg cgtttcgata gttttccgtg
     8281 taaaaattcc cgtccctgtc aagcacctcc gcatgggata tctctactat atctcccttc
     8341 attgcccgta cattgaatcg gacatagccg gtcacttcct gtccgaaatc aaccacgact
     8401 tcacccttcg gcgtacggaa aatccttttc ggacgtatac ggtccaactc ccggacgatt
     8461 tcgccttcct gcgggatcag ctgctccatg ccgaactgca ctgcacggac cggtagcaac
     8521 ggtgcctccg gcattgaaaa gtcggcatgc tcgccatcgt aaatctcact gaaacggatc
     8581 cggctttcac gtacctgcca gctttcgtcc gtaacgatct gtatcttatt accgctttct
     8641 gtctcaatat caagctgcgc aatgacagca gccgggagaa ggcttcgttg ctcataactg
     8701 tcagtgatcc accccgggat cggacttctg taccatccct tgccgaccag tacgcacaat
     8761 tcattttttt cagttaaaag ctctgtcacg tcgtagtcct gatattgatg acgtttttgg
     8821 taggaagtcc acccgggcgc caaaataaaa tcgcctgcac gctgtccgtt tatcgacgcc
     8881 tcatatacgc ccacggctgt aatatgcaaa atcgcttttt ttacaggtcc agcgatgtga
     8941 aaattcctgt aaaacaatgg gcatacatca cccataggtt tcgccggtgc tatccatttc
     9001 gccttccata tcatttgctt atccctccag ttttatagta ccttatgttg taagtttagt
     9061 atgacatcgg cagctgtctg tcagtacaaa aatctttcgg gcagccgcag gtcaatgccc
     9121 tggccggaca gtatatgttt caacgattgc aggaaacaca agcgttggtt tactgaacgg
     9181 gtttgataat gtcgtcaatt tagctcaact tcagtacata agttgcatta gaagtaaatc
     9241 tgcaaatact tctctcaaca tttctcttaa tattcataat tgtatcatca acgttgttga
     9301 gcaggaaata ttttataaaa tggccgggaa tgcccgtctt tcatcagcac aactttatct
     9361 gcacatgtac agatgccgac tctgtgagat tctattacag acgttttccc cctggtattg
     9421 ttgagaaagg ctttcagtat gtccatttca acaatcggat catggctgaa gtcggttcgt
     9481 caaggactac aaaatcactt tttttgaaca atgcccgggt ttttacgaac agtaaaggag
     9541 cagttcattt actgctcctc ctttttgcat ggcgccattt aaaaaatcaa aaattggtat
     9601 gtgcaacaat atctcaaact gagttgatca gtccatatat aaccatactg tcagcgatag
     9661 accttgattt ccatgcggcc agtcgaatta tagctcaccg gatgtttgcg ctgtcaggcc
     9721 atccgggcct tgcagttttt gtagaagatc tctatatcat cctgatcgcc gtttttgagt
     9781 ctcttatata ttttcgaaat cggatggcat gcaaaaaccc cttttctcac cgaaatacct
     9841 tttccttctt cctcctccca ttctccgcct atattcctga taatcaactc accaagattt
     9901 tcgtaaaagt tgattccagg ataagtgtcc ggagcatttc aatctggtca ccggtgagta
     9961 tatactcttt ttcatcttca tctcccacct ttattgtcac agtgcaatct gacgaaattt
    10021 gagaaactcc ttttatttca tcaccccgga cattgaacca ggagtaagtc aggactccaa
    10081 taatagctat tgaaataacc gcaattatta ttttacccct gcttttcatt ctttagctcc
    10141 ctcccaaggt atcagccgaa taaccgatct atgattcaac gtttgcagga aacacaagcg
    10201 ttggcttatc gaacgggtag tatcctttct ttatccataa cgggttgtaa ctccagatgt
    10261 ttttcaaaat agtccttgca tcgatcccgg cctcttttat ttccgattcc aggtactctt
    10321 tcgcagcccg gtccttttct cattcttttc actccggatt aagaatgttt gttatgtgtc
    10381 tgaaaccata ttcaaaacaa aggcccgaag taaatctatt atatcatcat tgttgttaaa
    10441 caggatcatt gttgttaaac aggataatat ttttatatta aaaagaacgc ttgttcttga
    10501 aataatcacc tgatataatg taagcgtata taatgtaagc gtacttcgtg caagtacgct
    10561 gcatttattg aaaggagcca ttattatgga tgaattcagg aagccttatg aatacatgga
    10621 agagaagggt ataagcggat tccttctggt ctattttctc atgcttctgg cacaggaaac
    10681 gctgctgggc gtgatcacac tgttcaacgg atatgacctg ctttccggga acagggtcct
    10741 tggtacgatc ataatgggaa tatccgtctt gtacatactg ttttcgctgt tctcggcgat
    10801 agtactcaag aagctcaaaa aattcgccgt cagagtctca aaggtattcc ttgtattcag
    10861 ggccgtctac ctgatccctt atcttatttt caatactatc agtcaaatca gggatatacc
    10921 atataacata gatcatgaga agtattcgaa tatgctcgga agcatcatcg tatcatccgt
    10981 cgtcagcttt ttgttaattg tagctttcag tgccggatgg tatatgtatc tgaacagatc
    11041 caaaaaagtc agggagttgt tccccgtcgg gatcgacgga tccaaagcgc cggt
//
LOCUS       DAOSCC010000065        10874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4009_length_10874_cov_4.072558, whole genome shotgun sequence.
ACCESSION   DAOSCC010000065 DAOSCC010000000
VERSION     DAOSCC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4009_length_10874_cov_4.072558"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLG72_05035"
     CDS             <1..430
                     /locus_tag="PLG72_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ80196.1"
                     /translation="VFSELKLAYPDRDIRLAIDTSLPRVCVDRILFRQLLQNVISNSM
                     KFTAVREKALITVGCTITQDNYVFYIRDNGVGFDMKYSGKLFGLFQRLHTSDEFEGSG
                     IGLVTVKKIIEKHDGKVWIDGKVDEGATVYFTLPLEASAQ"
     gene            516..2183
                     /locus_tag="PLG72_05040"
     CDS             516..2183
                     /locus_tag="PLG72_05040"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOJ80197.1"
                     /translation="MMNDRSKTALKTALIYMIAGVFWIILTDSLLGLLTANKSIFSWI
                     SIAKGLLFVAVSAAIVYVLVQNLLKRLMELELELLHTSNEMEGSKKEAATSYKQLIAI
                     KKKLYDMAYFDQLTGLRNQMSLHEDMGKIIGADRKFALVFMDVDNFKYVSDTLGHSFG
                     NLILKEISSKLAGLMEENCDLYRLAGDKFIVIYKDIRGILDIEQFALKILKSFKEAVV
                     VEDKSFYHSVSIGVSIYPEHGETINDLLKCAEIALFKAKESGRNRIVIYRESMVSAVN
                     EWVDTERYLRNALEKSEFELYFQPQYSIEKGEISGFEALIRWRNDEMGFVMPSKFLKV
                     AEDTNMIVSIGEWVLRNACIFLKRLQQEGFGDRTVSVNVSKMQILQDDFADSVLEIVE
                     IADIDPHNLEIEVPESILIEYYDLVAEKLNTLKNRGIKVVLDNFGRGYSALNYLRKLP
                     ITSVKLDKTFTDIITIGEDSKILTDFMIKIARSAKLQIVGDGVENREQFDYLSRNGCH
                     RLQGYYISRPLPEREAVRRLQESASKTGSNADKSNSTEPADTIDVKE"
     gene            complement(2409..2687)
                     /locus_tag="PLG72_05045"
     CDS             complement(2409..2687)
                     /locus_tag="PLG72_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80198.1"
                     /translation="MRTDQLLMSGLDITEDNYIFKGQFILSSEDKSKHVDMEELEKLP
                     YLEELKKHFDLEESPSEIRNILIDMVVEKAKIGSKIREGKNLEELHTK"
     gene            3171..3527
                     /locus_tag="PLG72_05050"
     CDS             3171..3527
                     /locus_tag="PLG72_05050"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOJ80199.1"
                     /translation="MSFFESVAVAAICMAVVFLVLAVLFALVQALSSVLGSAFRKGKS
                     NTEDASGMQHTAMKTAQDQTAVARDPQIGASVQASSLKLNNVDERTAAMIMAVVSHET
                     GTPLNELAFKSISLMK"
     gene            3579..3986
                     /locus_tag="PLG72_05055"
     CDS             3579..3986
                     /locus_tag="PLG72_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ80200.1"
                     /translation="MRYYVTINNVEYEVEVDNGKVGVMSTREVSQNTAGSAAASAEAK
                     AQPSENVQTKAPKPAPVDVSGGEAIKAPMPGTIIDVKVNAGQAVKRGQVILILEAMKM
                     ENEIVAPADGTVVQIQAVKGSSVNAGDVLAVLR"
     gene            4000..5139
                     /locus_tag="PLG72_05060"
     CDS             4000..5139
                     /locus_tag="PLG72_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ80201.1"
                     /translation="MFLDAINTLLQESGFAALTWQHCLMIGLACFLVYLAIVRKFEPL
                     LLLPIAFGMLLANLPIAGLMAGPQEGSNGPGGLLYYLYQGVKLGIYPSLIFLGIGAMT
                     DFGPLIARPSSLFMGAGAQFGIAAAFMIAIALGFKPGEAASIGIIGGADGPTAIYLTT
                     RLAPELLGPIAIAAYSYMALIPMIQPPLMRLLTTKKEREIKMEQLREVSKLERIIFPI
                     AVTVFCVLLLPSVAPLIGMLMLGNLFRESGVVERLSNTAQNALINIVTIFLGVTVGAT
                     ANAETFLRLQTLEIIGLGLLAFMFSTVGGLLIGKLMCWLSGGKINPLIGSAGVSAVPM
                     AARVSQIEGQKANPSSYLLMHAMGPNVAGVIGSAVAAGVLLALFG"
     gene            complement(5367..5440)
                     /locus_tag="PLG72_05065"
     tRNA            complement(5367..5440)
                     /locus_tag="PLG72_05065"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5406..5408),aa:Gln,seq:ctg)
     gene            5990..7657
                     /locus_tag="PLG72_05070"
     CDS             5990..7657
                     /locus_tag="PLG72_05070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ80202.1"
                     /translation="MSKSKQKLKVIPLGGLQEIGKNMTVFEYGDEILVVDCGLSFPED
                     DMLGIDHVIPDISYLAKNKEKVLGIILTHGHEDHIGAVPYFLKEINVPVYGTRLTLGL
                     LKYKLEEHGILPAAKLIDVEPGDTIRLGTFKVEFIRTTHSIADSAALALHTPVGIIIH
                     TSDFKIDYTPIEGKTTDLARLADLGRKGVLLLMCDSTNAERPGYTMSERTVGETFEDI
                     FMDAKSRIIVASFASNVHRIQQAIDAAAKFNRKVTVVGRSMMNVVNVAMELGYLNVPD
                     GMMVDVDLIDKYPPEKMVILTTGSQGEPMSALSRIAVNEHKKVEIEPGDLVIISASPI
                     PGNEKSIFKVINELFKKGADVIYESLADVHVSGHACQEELKLMHSLVRPRFFMPVHGE
                     YRHLKQHANLAAKMGMPPENIFMMENGQVLELGPDSARINGSVTSGRILVDGLGVGDV
                     GSIVLRDRKLLSQDGLIVVVITTEKESGQIIAGPDIISRGFVYVRESEDLMENAREII
                     KQTLAKLDGKRANDWAAKKNVIKNDLREYLYEKTKRKPMILPIIMEI"
     gene            complement(7872..8507)
                     /locus_tag="PLG72_05075"
     CDS             complement(7872..8507)
                     /locus_tag="PLG72_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ80203.1"
                     /translation="MEILSAISENLQKGKANTVRELVREALDKGVSAEKILEEGLISG
                     MDVIGTKFMNNEIYVPEVMIAARAMNAGMEVLKPVLVSAGVKSKGKAVIGTVKGDLHD
                     IGKNLVKMMLEGKGIEVIDLGTDVSAERFISAAVENGARIIACSALLTTTMPEMKKIV
                     EMLKEKGLRDKFTVMIGGAPVTDAYRQSIGADIYTPDAGSAAEAATKVFAE"
     gene            complement(8536..9765)
                     /gene="tyrS"
                     /locus_tag="PLG72_05080"
     CDS             complement(8536..9765)
                     /gene="tyrS"
                     /locus_tag="PLG72_05080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ80204.1"
                     /translation="MANNVFDTLKERGFIAQLTHEEEIKDLLSNNKVTFYIGFDPTAD
                     SLHVGHFVQLMVMAHMQRAGHKPIALIGGGTAMIGDPSGRTDMRKMMTRETINHNAEK
                     FKAQLSKFIDFSDGKAIMVNNADWLLDLNYIGFLREIGVHFSVNRMLTAECFKSRLER
                     GLTFIEFNYMLMQSYDFLILNKKYGCCMQLGGDDQWSNIIAGIDLIRRVEGKQAYGMT
                     FTLLTTSEGKKMGKTEKGALWLDAEKTSPYDFYQYWRNVDDADVIKCLKLLTFLPMDE
                     INRLAQLRDEQINEAKKVLAYEVTKLVHGEDEAMKAKQLAEALFGGGGSTSDMPSTSI
                     PLSELEKGINILDLLCRTGLVPSKSEARRLVQQGGIYIEGSQVSSIDHMICRDAFVNG
                     ELMIKKGKKTYHKVVIS"
     gene            complement(9986..10786)
                     /locus_tag="PLG72_05085"
     CDS             complement(9986..10786)
                     /locus_tag="PLG72_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80205.1"
                     /translation="MKYLSSAVDFAIKNWMLIIPVFVLLAISGLIQGAGAAASLGTIL
                     SLADMDNFTSAEAIFRLVPTILALTVGSGIVAFIARFIYEPATYGLVNKKLETGSATL
                     NEIGAAISENFVKYVMFLIGSLVVNLVLGIATILILLLMVLLVSLLKGFGVALMVIVI
                     IALVLFFIAFGVLISLWFSAMVVDGLSVFDGFKKSIEIVRTCFWTVLGITILVSLAAS
                     VVSAILGFLGAIPVLGPVILSVIPAAQYFVIAVFSLLIYRDKTGRAIA"
BASE COUNT         2849 a         2460 c         2804 g         2761 t
ORIGIN      
        1 agtgttcagc gaactgaagc tggcatatcc ggacagggat atcaggctgg ccatcgatac
       61 cagtttgccg cgtgtttgtg tggaccgtat cctgttcagg cagctgctcc agaatgtgat
      121 atcgaactcg atgaagttta ccgccgtcag ggagaaggct cttataactg tcggctgcac
      181 catcacacag gataattatg tcttctatat cagggacaac ggcgtcggct tcgatatgaa
      241 gtattcgggc aagcttttcg gattgttcca gaggctgcac acatccgacg agtttgaagg
      301 cagcggcata ggacttgtta ctgttaagaa aataattgag aagcatgacg gaaaggtttg
      361 gatagatggc aaagtggatg aaggggcgac cgtgtatttc acattgccgc tggaagcttc
      421 agcccagtga tccgcctggt ttgcgccagg acggcataag ccccatggcg ccttaaggtt
      481 ctatagtgtc tggaaagaaa ggatggtcat gatctatgat gaatgacagg agcaaaacag
      541 cattaaaaac tgcgttgatc tatatgatcg caggcgtttt ctggataatc ctgaccgaca
      601 gcctgttagg cctgttaaca gccaacaaaa gcatattttc gtggatcagc atagctaaag
      661 gcttgctttt cgttgccgtt tcggcagcca ttgtttacgt gctggtgcag aacctgctca
      721 aaagactgat ggaacttgag ttagaactgc ttcacaccag caatgagatg gaaggttcga
      781 aaaaggaagc ggctacgtcg tacaagcaac ttatcgcgat aaaaaagaag ctgtatgata
      841 tggcatattt cgaccaactg acgggactgc gcaaccagat gtcgcttcat gaggatatgg
      901 ggaaaataat cggcgccgac aggaaattcg cgctggtttt tatggacgta gacaatttta
      961 aatatgtaag tgacacactg ggacactctt tcgggaacct gatactcaag gagataagca
     1021 gcaagcttgc cggactcatg gaggaaaact gcgacctgta cagattggcc ggagacaagt
     1081 tcatcgttat ttataaggat atcaggggca tcctggatat cgaacagttt gcgcttaaaa
     1141 tactcaagag cttcaaagag gcggtggtcg tcgaggacaa atcattctac cattctgtga
     1201 gcataggcgt ttccatatac cctgaacacg gcgaaacgat caatgatctg ctcaaatgcg
     1261 ccgagatagc tcttttcaag gccaaggagt ccggcaggaa caggatcgtc atttaccgcg
     1321 aatccatggt ttccgctgta aatgaatggg tggatacaga gaggtacctg aggaatgcgc
     1381 ttgagaagag cgagttcgag ctttacttcc agccgcagta cagtattgag aaaggtgaga
     1441 tctcgggatt tgaggcgctt atcaggtgga ggaacgatga aatgggcttc gtcatgccga
     1501 gcaagttcct gaaagtggcg gaagatacca atatgatagt ttcgatagga gaatgggtgc
     1561 tgaggaacgc atgtatattc ctgaaaagac tgcagcagga gggattcggc gacaggactg
     1621 tttccgtgaa tgtgtcgaag atgcagatac ttcaggatga ttttgcagat tcggtccttg
     1681 aaatcgtcga aatcgccgat atcgatcctc acaatcttga gatcgaggtg ccggaaagca
     1741 tcctcatcga gtattacgat ctggtggccg aaaagttgaa cactctcaag aaccgcggca
     1801 tcaaggtcgt tctcgataat ttcgggagag gctactctgc gctgaattac ctgcgcaagc
     1861 tgcccattac ttcagtaaag ctggacaaga catttaccga tatcataacg atcggagagg
     1921 acagcaaaat attgacagac ttcatgatca aaattgccag gagcgcgaaa ctgcaaatcg
     1981 tcggcgacgg agtggagaac agggagcagt tcgattacct gagccggaac gggtgccaca
     2041 ggctgcaggg atattacata agcagaccgc tgccggaaag ggaagcagtg aggagactgc
     2101 aggagtctgc gtccaaaacc ggcagcaatg ctgacaaaag caattcaaca gaaccagcag
     2161 atacaattga tgttaaggaa tgagccaatg cagcgatttt agtattcaac tgatgagatg
     2221 cagggacgat ttcacaatga agccgcagca tgagatggat ctcatggcta tcatgatttc
     2281 aattagccgg gcaggcaaag caacaaagca ggcaccgcaa actggaacac agttttcaga
     2341 tgcggtggcc tggcatgcag catccggatg agaccgggtt tttatgtgat cggagtcgaa
     2401 acctcttttt attttgtgtg cagttcttcg agattctttc cttcacgtat ctttgagcct
     2461 attttcgctt tttcgactac catatctatt aatatgtttc ttatctcaga aggagactct
     2521 tccaggtcaa agtgcttttt cagttcctcc aggtacggca gcttttccag ttcttccatg
     2581 tccacgtgtt tggatttgtc ttcactgctc aggatgaatt ggcctttgaa aatataattg
     2641 tcttctgtaa tgtcaagacc tgacatcagc agttggtctg ttctcattaa tataccccct
     2701 tatcacagtt tattagatat gtgggtcaga tataccgccg ggcacatgga tgcataccat
     2761 tgatgcaaac aggaagtgta gtttaaaagc atagttatta cttatatttt aacatctttt
     2821 ttcccgtttg aaaaccgcta acatacggat ggctgcatgg acgattatac caggtgtttc
     2881 agcgccatga tcccggggaa actcccggat tcgtgaaaaa ttttctgtgt catcggagtt
     2941 tgaacggggc gtgagacgaa tggacattta tcgccttgtc attattgact tttaagcctt
     3001 ttatccttat aatgatactg ttgtgtgtga ttgtaccata gtagacagaa gcatgaagag
     3061 aactggtttg gttcttttta tttgttcata attgacaaaa aaataacatg tttgggcaac
     3121 acagtataca gtaatatatt caaaaatgaa atagtaaggg agggtgccaa atgtcatttt
     3181 ttgagagtgt ggctgtcgct gctatttgca tggcagtcgt ttttttggtg ctggctgttt
     3241 tgtttgctct tgtgcaggca ttgtcaagtg ttttgggttc tgccttccga aagggcaaaa
     3301 gcaatacgga agatgcgtct ggaatgcagc atacggccat gaagactgcg caggatcaga
     3361 ctgctgttgc acgtgatccg caaataggcg catctgttca agcaagcagt ttaaaactca
     3421 acaatgtcga tgaaagaaca gcagccatga ttatggcagt cgtgagccat gagaccggaa
     3481 caccgctgaa tgaactggcc ttcaaatcta taagcctgat gaagtaaaaa ggctgctgta
     3541 acggctggtc gcagcttttg taaaggagcg aggtaaatat gagatattat gtaacaatca
     3601 ataatgttga atatgaagtc gaggtcgata atgggaaagt cggtgtcatg agtaccaggg
     3661 aagtatcaca gaataccgca ggctcggcag ccgcctctgc agaggcaaag gctcagcctt
     3721 cggaaaatgt gcagacaaag gcgccgaagc ctgcgcctgt tgatgtaagc ggcggcgaag
     3781 ctataaaggc gcctatgcca ggaaccataa tcgatgtgaa agtcaatgca ggtcaggcag
     3841 ttaagagagg tcaggttata ttgatccttg aagcaatgaa aatggaaaat gagatagttg
     3901 cacctgcaga tgggacagtt gtacaaatcc aggccgtcaa ggggtcatcc gtaaacgccg
     3961 gggacgtcct tgcagtgctc agatagagag gtgatcgaca tgtttttgga tgcaataaat
     4021 accttattac aggaatccgg atttgctgcg cttacctggc agcattgcct gatgatcggt
     4081 ttggcttgtt ttctcgtata tctggcaata gtaaggaagt ttgaaccgct gctgcttttg
     4141 ccgatagcct ttggcatgct gctggcaaat ctgcctatcg cagggctgat ggccggaccg
     4201 caggaaggtt cgaacgggcc tgggggactg ctgtattacc tgtaccaggg agtcaaactt
     4261 gggatatatc cgtcgctgat cttccttggc atcggggcga tgaccgattt cgggccgctg
     4321 atcgcaagac cgtccagcct tttcatgggt gccggagcac aatttggcat agcggctgcg
     4381 ttcatgatag cgatcgcatt gggctttaag cctggggaag cagcttccat cgggataata
     4441 ggaggcgctg acggaccgac cgcaatatac ctgacgacca ggctggcgcc tgaacttttg
     4501 gggccgatag cgatcgcggc atattcctac atggcgctga taccgatgat acagccgccg
     4561 ttgatgaggc tgcttaccac gaagaaggaa agagaaataa agatggaaca gctgcgcgag
     4621 gtttcgaagc ttgaaaggat aatattcccg atagctgtca cagtattctg cgtgttattg
     4681 ctgccctccg tcgcaccgct tatcggcatg ctgatgctcg gcaacctttt cagggaatcc
     4741 ggagtggtag aaagactttc aaacactgct cagaacgcat tgatcaatat cgttacgatt
     4801 ttcctcggag taactgtagg cgcgacagcc aatgccgaga ctttcctcag actccagacc
     4861 ctcgagataa tcggcctggg acttttggca tttatgttca gcacagtggg aggattgctt
     4921 ataggaaagc tcatgtgctg gctctcgggc ggaaagatca acccgctgat cggttcggca
     4981 ggagtttccg ctgtgcctat ggcagccagg gtctcgcaga tcgaaggtca gaaggccaat
     5041 ccgtccagct acctccttat gcatgccatg ggcccgaacg tagcaggcgt aataggctct
     5101 gctgtcgcag caggagtact gctggcgctg ttcggatgat acagatacag gatatataca
     5161 gacgggacgg ctcgggccgt cccgttttgc ttttcatggg ggacagttct gcagaagctg
     5221 aggtacggtc cggggaagag ggacatttct tcaagcatat cttaactgat ctgtatccgg
     5281 aattataaat tgttatactc agacaccgcc gcaccgggaa gataaaaaaa caaaggatac
     5341 gcggatcctg cgtatccttt gctcactggc tgcgggagaa ggattcgaac cctcacagac
     5401 tgatccagag tcagtagtgc tacccttaca caatcccgca acgtttgcga cagtcgttat
     5461 tatagcacat tgtccatggg ttatgcaata gttttttcaa aactgttatg cttgaccgcc
     5521 gcttttgtga aatcccccga aaaagtaata tagagcccat ccgggcccca taccagctaa
     5581 atgatattca tccgggtaaa acaaaagaga gtcgttcctt gaacgagtaa gtccattata
     5641 ctagtacaat aatcctatgt caataagaat aaagacctat ttttccccga caaatggaag
     5701 gtgttgacac cacaatcaaa taagtatttt gcaaaaatat cttgtaatgc tataataggt
     5761 aaagtggcgg caggacagcg gttcagaagt gacggataca gccggacaga cagttttcgt
     5821 ttggaatggg tctgcacaga gcagcaggtt tgcggatcat gagcggcaac tgtgcagtgc
     5881 atcatgcgtt gcattgaatc ctgatacata ttgccagatg atttgttgaa aatattcata
     5941 atggtggaat tatttataaa gaataagagt tatttacgga ggttatatcg tgtcaaaaag
     6001 caaacaaaag cttaaagtca tccctttggg aggattgcag gaaataggga agaacatgac
     6061 ggttttcgaa tacggagacg aaatactggt ggtcgattgc ggactttcgt tccctgaaga
     6121 cgatatgctt ggaatagatc atgtcatacc tgacatatct taccttgcga aaaataaaga
     6181 gaaagtcctt ggtataatcc tgacacacgg ccatgaggac catatcgggg cagtgcctta
     6241 ctttttgaag gaaataaacg tgccggtata cggcaccagg ctgacgctcg gactgctgaa
     6301 gtataagctg gaggagcacg ggatactgcc ggcggcaaag cttatcgacg tggaacctgg
     6361 cgatacaata cgcctgggga cattcaaggt ggaatttata aggaccaccc acagtattgc
     6421 cgactcggcg gcactggcgc tgcatacgcc cgtggggatc atcatccaca cgtctgattt
     6481 caagatcgat tatacgccga tagagggcaa gacgaccgac ctggcacggc ttgcagatct
     6541 cggcaggaag ggcgtgctgc tgctgatgtg cgacagcacg aacgcggagc gtccaggata
     6601 cacgatgtct gaaaggacag tgggcgaaac gttcgaggat attttcatgg atgcgaagag
     6661 caggatcatc gtggcaagct tcgcatccaa tgtccacagg atccagcagg ccatagacgc
     6721 ggccgccaag ttcaaccgga aagtgaccgt cgttggcagg agcatgatga atgtggtcaa
     6781 tgtggcgatg gaacttggat atctcaatgt gccggatggg atgatggtgg acgtggacct
     6841 gatcgacaag tatccgccgg aaaagatggt catcctgacg accggcagcc agggagagcc
     6901 gatgtccgcg ctgtcgagga tagctgtcaa cgagcacaag aaagttgaga tcgagccggg
     6961 cgacctggtg attatttcag cgtcgccaat accgggaaat gaaaagtcca ttttcaaggt
     7021 cataaacgag ctgttcaaaa aaggcgcgga cgtcatatat gaatcgctgg cggatgtaca
     7081 tgtgtcgggg catgcatgcc aggaagagct gaaacttatg cacagcctgg tcaggccgag
     7141 attcttcatg ccggtccacg gcgaatacag gcacctaaaa cagcatgcga acctggcggc
     7201 taaaatgggg atgccgcctg aaaatatctt tatgatggag aacggccagg tgctcgaact
     7261 gggtcctgat tcggccagga tcaacggaag cgtcacatcg ggcaggatac ttgttgacgg
     7321 cctcggtgtg ggcgacgtcg gaagcatcgt gctcagagac aggaaactgc tttcgcagga
     7381 tgggctgata gttgtggtca ttacgacaga gaaggaatcc ggacagatca ttgcgggtcc
     7441 cgatatcatt tccaggggat tcgtatatgt cagggaatct gaagatctga tggagaatgc
     7501 ccgtgagatc ataaaacaga cgcttgcgaa actggacggg aagagggcca acgactgggc
     7561 cgccaagaag aatgtcatca agaatgacct gagagagtat ctgtatgaga aaaccaagcg
     7621 caaaccgatg atattgccta tcataatgga gatataaaga aaaggtgaca ctccccggca
     7681 aggcatgcgc ggcattcccc ggccttacag ggaatgcttc catggaaggg agcgtcacca
     7741 gtctttttct gtgaacagtg cctctgaaat aaacatgccc ggcatgtcat atgttcacat
     7801 attatccggc aggatgccga gcaatatgcg tccatgtgac gtaccaggca ctaaaatcat
     7861 cagcatccga attactcagc aaataccttt gttgctgcct ctgctgctga tccggcgtcg
     7921 ggtgtgtaaa tatcggcgcc gatggattgc ctgtatgcgt ccgtgacggg agcgccgcct
     7981 atcattacgg tgaacttgtc cctgaggcct ttttccttca gcatttcgac tatttttttc
     8041 atctcgggca tcgttgtggt aagcaatgcg gaacatgcga taatacgggc gccgttttct
     8101 actgctgcgg aaatgaaccg ttcagcagat acatcggttc caagatcgat gacttcgatg
     8161 cctttgcctt ccagcatcat tttgaccaga ttcttgccga tgtcatgaag gtcgcccttt
     8221 acagttccga tcacggcttt gcctttcgat ttgacgccgg cgctgaccag cacaggcttc
     8281 agcacttcca ttcctgcgtt catcgctctt gcggcgatca taacttccgg cacatatatc
     8341 tcattattca tgaatttcgt tccgataacg tccattccgc tgataaggcc ctcttccagg
     8401 atcttttcgg cagaaacgcc tttatccaat gcttcccgga ccagttccct tacggtattt
     8461 gctttacctt tctgcaggtt ttccgatata gcgctcagta tctccaatca aagacctcca
     8521 atcttccatg cttcatcatg aaatgactac tttgtgataa gtcttcttgc cctttttgat
     8581 catcaattcg ccgtttacaa atgcatccct gcagatcata tggtctatcg aagaaacctg
     8641 gctgccttcg atatatatgc cgccctgctg taccagcctg cgggcttcgc tcttcgacgg
     8701 tacgagcccg gtcctgcaaa gcaggtcaag tatattgatc cctttctcga gttccgaaag
     8761 ggggatcgag gtcgacggca tgtcgcttgt actgcctccg cctccgaaca gtgcttccgc
     8821 aagctgtttt gccttcatgg cctcatcttc gccgtgtacc agcttcgtta cctcgtatgc
     8881 cagcactttc ttggcttcat tgatctgttc gtccctgagc tgtgccagcc tgtttatttc
     8941 atccatgggc aggaatgtca gaagcttaag gcattttatg acgtcggcgt cgtcgacgtt
     9001 cctccagtat tgatagaaat catacgggga agttttttca gcatccagcc agagggcgcc
     9061 tttttccgtt tttcccattt tcttgccttc gctggtggtg agcagggtga acgtcatgcc
     9121 gtaagcctgt ttgccctcga cacgccttat caggtcgatg cctgcgatta tgttggacca
     9181 ttggtcgtct ccgccgagtt gcatgcagca gccgtatttt ttgttaagga tcagaaagtc
     9241 ataactttgc atcagcatgt aattgaattc gatgaaagtc aatcctctct ccagccttga
     9301 cttgaagcac tcggcggtca gcatcctgtt gactgagaag tggactccta tttctctcag
     9361 gaacccgata tagttgagat ccagcagcca gtcagcattg ttgaccataa tggctttacc
     9421 gtcggaaaag tcgatgaatt ttgacaactg ggccttgaat ttttccgcgt tgtggtttat
     9481 cgtttcccgg gtcatcattt tacgcatatc ggtcctgccg gacggatcgc cgatcatggc
     9541 cgtaccgccg cctatgaggg ctatgggctt atgtccggcc cgctgcatat gcgccattac
     9601 catcaactga acaaaatggc ctacatgcag gctgtctgcg gtcggatcga agcctatgta
     9661 aaatgtgacc ttgttgttgg aaagaagatc ctttatctct tcttcatggg tgagctgggc
     9721 gataaagccc ctttctttca gagtatcgaa aacgttgttc gccatagtta ccttccttta
     9781 ccctttatag ctgtcttccc tttagttttt gagtatttta ccatactgta tacagttatt
     9841 caagttcagt tacagacctt atggcccgtc gcggcggatg ccattcaggc ctggaccgga
     9901 tctgtgcatg aaaaaaggga tacataggta tccccgagtt atcgtactaa ttcagctttg
     9961 tctggcaagt tcggccatat atgtatcatg cgatcgctct tccggttttg tcacggtata
    10021 tcaacagtga gaacactgca atgacgaaat actgggcagc cgggatgacg ctgagtatca
    10081 ccgggccaag taccggtatt gcgccgagga agccgagtat tgcacttacg acgctggcgg
    10141 ccagggagac cagtatcgtg atgcccaata ctgtccagaa gcatgttctg acgatttcga
    10201 tggatttttt gaaaccatcg aatacactca gtccgtcaac caccattgct gagaaccaaa
    10261 ggctgatgag cacgccgaat gcaatgaaga aaagtaccag tgcaatgatc acgatcacca
    10321 tcagcgctac gccgaaacct ttcagcaatg acaccagaag gaccatcagc agcaggataa
    10381 gtatcgttgc gatgccgagg accaggttca ccaccagcga tccgataagg aacatgacat
    10441 actttacgaa gttttcgctt attgccgctc cgatctcgtt cagggtagcg cttcctgttt
    10501 cgagcttttt gtttacgaga ccataggtgg caggttcata gatgaaccgg gcaataaatg
    10561 ctacgatgcc gcttccgact gtcagtgcca gtatggtggg gacaagtctg aatatggctt
    10621 ccgcactcgt gaagttgtcc atatctgcca gcgaaaggat agtacccagg ctggctgcag
    10681 ctcccgcacc ctgtatgaga ccggatatgg ccagcaaaac aaatacgggg atgatgagca
    10741 tccagttttt gatagcaaaa tcaactgcac tagataaata cttcatggta cacctccatc
    10801 atgtttatat atacctattc tatatggcgg aaaaaatcct tccattaagc aaaaaaataa
    10861 aataacattt gtaa
//
LOCUS       DAOSCC010000066        10753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4055_length_10753_cov_4.589549, whole genome shotgun sequence.
ACCESSION   DAOSCC010000066 DAOSCC010000000
VERSION     DAOSCC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_10753_cov_4.589549"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLG72_05090"
     CDS             complement(<1..1283)
                     /locus_tag="PLG72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80206.1"
                     /translation="MDKEKVFRVVLVAALVFLIYQFGFRPIFNRGSEDINIKADRKYW
                     SKLLPDEEGPVYDVIVYGAEPQGIAAAISSARLGARTLLISEDYDMGGIITRCLIPEL
                     EVPLDSSGKMLNGGFLEELYKKTGDCFSPEEYTEAVNELIQAESDLSAIAGSVVTGVN
                     MTGRHIDSIEVLTKDGKADLSARMYIDASDSGALLELCKVPYFTGSADLGLENRFMPV
                     SLNFEMELKAGSAADGKNLAGRTAVFNDGPVKYEPLNKNIRLEDPRICFLSDGKVIIR
                     GLQVAEVDVLDGDSMQRAYGTAVEEAKNLSRWLSENYVELKDHVFSKAAPSLRVKESK
                     HYKGKYVLSVDDILGGRYFDDTVAMGSHPIMISKFAASGTFIAADPDQYAIPLGSLVP
                     DGVLNLLMVGPRISFSSLAASSASAIGTCIAQGEAA"
     gene            complement(1419..1769)
                     /locus_tag="PLG72_05095"
     CDS             complement(1419..1769)
                     /locus_tag="PLG72_05095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ80207.1"
                     /translation="MIIKRGDIFYADLSPVIGSEQGGVRPVLIVQNDIGNKYSPTVIA
                     AAITSQINKAKLPTHIEISAQEYGLQKDSVILLEQIRTIDKKRLRERIGHLDDELMEK
                     VNEALSISFGLADI"
     gene            complement(1772..2047)
                     /locus_tag="PLG72_05100"
     CDS             complement(1772..2047)
                     /locus_tag="PLG72_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOJ80208.1"
                     /translation="MAELKKILISLPDNLLKEIDHIVSVEKTNRSEFVREAMKLYLRE
                     KRKIEMRERLKKGYLEMAEINMRLAEMCMEADSEQQLKYEERLGELE"
     gene            complement(2353..3519)
                     /gene="alr"
                     /locus_tag="PLG72_05105"
     CDS             complement(2353..3519)
                     /gene="alr"
                     /locus_tag="PLG72_05105"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ80209.1"
                     /translation="MEYKLNRAWAEIDLDAIAYNTREIKKLTGKNVEMMGVVKADAYG
                     HGVLEVVSTLLDNGVTQLAVSMLDEAIQIRKMGIGVPILILSYTDPARADEVILNEVT
                     QTVFSLDLAEALSAAAVRLGRKAKIHVKVDTGMSRVGFMPGHTAVKSIMEIAKMPGIV
                     IEGIFTHFASADEADRSYTMMQFERFMSLYDELEKAGIHIPVRHACNSGGILQYPEMH
                     LDMVRPGIILYGLYPSKDVDWKIDLKPAMTLKAKVIYVKDVDRGVCISYGRTFRTARK
                     SRIATVPIGYADGYTRLMSNKGKMLVNGEFAPVVGRICMDQCMLDVTDLEHEVHVGDE
                     VVIFGKQNGACISVDEIAEQVGTINYELVCIIGKRIPRVYLKGGKICSVLNYLI"
     gene            complement(3540..5126)
                     /locus_tag="PLG72_05110"
     CDS             complement(3540..5126)
                     /locus_tag="PLG72_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ80210.1"
                     /translation="MKVVSPGQMKKIDECTVNEYGIPGILLMENAAAAVAAQARAMMG
                     GCAGRLVTVVAGRGNNGGDAFAAVRLLHSKGADARVYLMGEKKGLSGDALFNMEILER
                     IGFGITELTEETGLDGFVSDLAVSDLILDGLFGTGLSRDITGFAAEIIERINASGRPV
                     LSIDIPSGIDGRDGSIKGVCVKADATVTFGLPKLGLILHPGCRYTGELITADIGIPAC
                     VLEKQDIKTETIDLDMVSAMIPRRKPDSNKGDYGRVLIITGSTGMTGSGCLASMSALR
                     TGAGLVYAGVPKSLAPVYSSKMTEPIVIPLEDNGTGRLSASCVEHILGLMDKMDVAAI
                     GPGLTAGDDIRKIVEAVIENSKVPLVIDADALNAISADTSVLRKLKTDAVLTPHPGEM
                     ARITGTNTKQVQSDRMGTALNFAQQYGVAVVLKGSRTIVAYPDGRVLINTTGNAGMAT
                     AGTGDVLTGMIAGIAAQGVPVGDAAAAGVYLHGLAGDAAAELKGMHGMVAGDLVDILP
                     ITIKEVLKETWRMLDGGHGT"
     gene            complement(5414..6055)
                     /locus_tag="PLG72_05115"
     CDS             complement(5414..6055)
                     /locus_tag="PLG72_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80211.1"
                     /translation="MPLPKIFPKKLRQMRMKLKFDAGTLRRNDISLLILDERWNKLFT
                     NTQKTPEIERCEQKLRELMKEEARLLAEQKEIAAAKKHHLSRIMELTTEAFEKGSEAA
                     RDEMKASEQEIKRINARAKRIEEELEEIPSRIRDANIELLELTVMVVYYKIRSGRKRV
                     EELEKLIEETKAKLKEYIDEKEALSQDDSDIYSYFHDLLGGEELEKIDRMFFK"
     gene            complement(6062..7261)
                     /locus_tag="PLG72_05120"
     CDS             complement(6062..7261)
                     /locus_tag="PLG72_05120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOJ80212.1"
                     /translation="MKAGILTFHYAHHYGAQLQAYALMRAIQLLGVDCEIINYVRKDN
                     IEGSSLFKKGLSPASILSNADTLLHYGSLKKRHDRFDSFVADEMRLSGKFYGSYKELC
                     DDPPAYDVYVCGSDQIWNPFIFSEKTYDPAFFADFAVNGRRVAYAPSFGVSSIPEDKR
                     EELARYLEKFDSLSSREKQGEKIIREITGREAVTVLDPTLLLPAEEWGKIASAAEACG
                     AYPGTGEKSAKADSGRQNAGGGYILCYFITDARKYADYVGAVAGKYGLPVVSLCGSRR
                     VVPQTKYKRLDCGPREFLGLFAGASAVLTDSFHGTVFSLIFEKEFYSFEASRDPSKAV
                     GSRQHNILEVTGLLSRLYHLSSGSDGFRKLLGGQTGAIDYLTVREILQKKREESLSYL
                     KNALTGR"
     gene            complement(7349..8869)
                     /locus_tag="PLG72_05125"
     CDS             complement(7349..8869)
                     /locus_tag="PLG72_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80213.1"
                     /translation="MRTKLSFYNSISAVALQLVNIIVNLILPRVMITVYGSNVNGLVT
                     SIRQFISYFNLVEAGLAGAAVYALYKPLAEKNSDDINGILSASNRFYNISGYIFSALV
                     VVSAFIYPMLTSGQGIDVFTIAALVLIIGASGAMEFFAVGKYKVLYTADQKSYVISLI
                     NAAAVAMNAVIILILASFRLNIVIVQLAALTSFFARSALYVIYGRLKYKDIDFSAPPK
                     NEALDKRWDALVLQILGAASTATPVVVTSLFVSLSEASVFVTYNMVFTSVLALLTTFS
                     NGLSAIFGDMLARNELPTLQKAYHQYEYLYYALVAWGYSVAAVMIMPFMEIYTAGFTD
                     AEYIRPTVAAMFVATGIITNIKNPQGMLVISAGLYRETRLQSLIQALIIVVASVLLAP
                     YFGIVGVLTGAILSNLYRDIDLFFYIPKTVTKLSPFMTIRRVIRLVVLFFITVLPAVY
                     FADINTGGYIEWALYAVAVGIWALLVIGAGNILMERKTAAAVYERVRQLARNRAGK"
     gene            complement(8879..10339)
                     /locus_tag="PLG72_05130"
     CDS             complement(8879..10339)
                     /locus_tag="PLG72_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOJ80214.1"
                     /translation="MEQIIYNIRYCTGCSACSQVCPRGAVAMEADDEGFIFPKINGER
                     CNDCGLCRRTCPVNAAFEKTGSIVTDTDGTSVFTAGRSSVPVFGSRENSAAESVRDET
                     DGGCRKSTDGSASNFGGMRAFACCSKNDGTREKSSSGGIFSELAAKTLEDGGAVFGAA
                     FDSEFNVKHKCIESIGELDDLRRSKYVQSEIGETFKEAEKYLKEGRKVLFCGTPCQIA
                     GLKAYLGKKYAGLTACDLACFGVPSPKVWRMFVDYLGKRYNSRISSVSFRDKSGGWRE
                     SSVSIGFENGGRYVVSVKKELYLMGFFKNLYTRRSCFDCRFRIQNSQADITLADFWGI
                     EKLKGLAKRKGPAATDGGTMLMDSDRTVKGDDIATIDGDTLPEAGDTRAREDDTAVRD
                     DNRGISLVITHTKAGEEALFGISDRADIVEYAPEDAVRYNPRLVSSLPEPAGRARFFA
                     DMKNGHSFDRLRRKYMDNTSIKYQVKCIIKKVLGRG"
BASE COUNT         2201 a         3165 c         2512 g         2875 t
ORIGIN      
        1 gcggcttctc cctgcgcgat gcatgtgcct atggcactgg cgctgctggc agccagggac
       61 gagaacgaga tcctcgggcc aaccatgagc aggttcagca ccccgtctgg cacgaggctg
      121 ccaaggggta ttgcgtattg gtccggatct gcggcgataa aggtgccgct ggcagcgaat
      181 ttgcttatca ttatcgggtg ggaacccatt gcgactgtat cgtcaaaata cctgccgccg
      241 aggatgtcat cgacgctcag cacatatttg cccttgtaat gctttgattc ctttactctc
      301 aggctgggcg ccgcttttga gaagacatgg tccttcagtt ccacgtaatt ttcggaaagc
      361 cagcggctca ggttcttcgc ttcctcaaca gcggtcccgt aagcgcgctg catgctgtca
      421 ccgtccagca cgtcgacctc cgccacctgc aaacccctta tgatcacttt gccgtcggaa
      481 aggaagcaga tccgcggatc ttcaagcctg atgtttttat tcagcggttc gtatttgaca
      541 ggcccgtcat tgaatacggc tgtcctgccg gcaagatttt tgccgtcggc ggcgctcccg
      601 gccttcagtt ccatttcgaa attgaggctg acaggcatga acctgttttc cagtcccagg
      661 tcggcagagc ctgtgaagta cggcaccttg cacagttcta gcagcgcccc gctgtctgac
      721 gcgtctatgt acatccttgc cgaaaggtcc gctttcccgt ccttcgtcag gacttctatc
      781 gaatcgatat gcctgccggt catgttcacg cccgttacaa cggaacctgc aatggcggaa
      841 aggtcgcttt ccgcctgtat gagttcgttt accgcttccg tatattcctc cgggctgaag
      901 cagtcgccgg tttttttata cagctcttca aggaaccctc cgttgagcat tttgccgctg
      961 ctgtccaggg gcacttcaag ctccgggatg aggcaccggg ttattatgcc tcccatgtcg
     1021 taatcttcgc ttatgagcag tgtgcgcgcc ccgagccttg cggacgatat ggccgcagcg
     1081 atgccctgcg gttccgctcc gtatacgatg acgtcataga caggcccctc ttcatcgggc
     1141 agcagtttgg accagtattt cctgtccgcc tttatgttta tatcctcgct ccccctgttg
     1201 aatatcggcc tgaatccgaa ttgatatatc agaaaaacca atgccgcaac caaaacgacc
     1261 ctgaatactt tttccttatc catatcaaaa gatcccctca tatggcccgg catcgcctgc
     1321 cgtgccgctg cggcagtatg tttcagattt aaggcttgtt tttgtatgta tagcgggttt
     1381 ccttcacatt tcctgcatct gcgccttatg ccgccaattt atatatcggc cagaccaaag
     1441 cttatgctta gcgcttcatt aactttttcc atcagttcgt cgtccagatg ccctatacgt
     1501 tccctgagcc ttttcttgtc tatggtcctg atctgctcga gcagtatcac cgagtccttc
     1561 tgcagcccgt actcctgcgc gcttatctcg atgtgggtcg gcagcttcgc cttgttgatc
     1621 tgcgacgtta tggccgccgc tatcaccgta gggctgtact tgttgccgat gtcgttctgc
     1681 actatcagta cgggcctgac tcccccctgc tcagagccga tcacgggact cagatcggca
     1741 tagaaaatat caccgcgttt tattatcacg tttactccaa ctccccaagt cgttcctcat
     1801 attttagctg ctgttcgctg tccgcttcca tgcacatctc tgccagcctc atgtttatct
     1861 cggccatttc caggtaacct ttcttaagcc tctctctcat ctctatcttt ctcttttccc
     1921 gcaggtacaa tttcatcgct tctctgacaa actcgcttct gttggtcttc tcaacagaca
     1981 cgatatggtc tatttcttta agcagattat caggaaggct tataagtatt tttttcaatt
     2041 ctgccaaaat aaatccccca tttttcgatc gaattgcaat ccgttcctga gaagataccg
     2101 tccagccagg cagcgcacaa cagcagcact taaataattc tttgctgccc tcaccaaaaa
     2161 tatgtggtag tatatacttc attatatacg atagtatata cccggtcaaa ttacattact
     2221 gttaccgata taaagccgca tataactctg tcgcagaaat aaaaaaaacc tgctgcgatt
     2281 taaatgcaag cagttataaa cctgctgctg taatacatgc ggcaggaact gctattaaaa
     2341 tattattcag gactaaatga gataattcag gacgctgcag atttttccgc ctttaaggta
     2401 gacgcgcggt atccttttgc ctatgatgca gaccagttcg taattgatgg tccccacctg
     2461 ttccgctatc tcgtcgacgc tgatgcatgc cccgttctgt tttccgaata tgacgacctc
     2521 gtcccccacg tgcacttcgt gctccaggtc ggtcacgtcg agcatgcact ggtccatgca
     2581 gatcctgccc acgaccggcg cgaattcgcc gttcacgagc atcttcccct tgttggacat
     2641 gagcctggta tatccgtcgg catatccgat gggcacggtg gcgatcctgc ttttccgggc
     2701 ggtccggaat gtcctaccgt agctgatgca tacgcccctg tcaacgtcct tgacatagat
     2761 aactttggct ttcagcgtca ttgctggctt cagatctatt ttccagtcca cgtctttaga
     2821 agggtacagt ccgtaaagta tgatgccggg cctgaccata tcaaggtgca tttccgggta
     2881 ttgcagaatg ccgccgctgt tgcatgcatg ccttaccggg atgtgtatcc cggctttctc
     2941 aagttcgtcg taaaggctca tgaagcgctc gaactgcatc atcgtatagc ttctgtcggc
     3001 ctcgtcggcc gaagcgaaat gcgtaaagat accttcgatc acgatacccg gcatctttgc
     3061 tatctccatg atgcttttca cagcagtatg ccccggcatg aagcccaccc tggacatgcc
     3121 ggtgtcgacc ttcacatgga tcttcgcttt cctgccgagc ctgacggccg cagcggacag
     3181 cgcttcggcg agatccaggc tgaaaactgt ctgggtgact tcgttcagta ttacttcatc
     3241 cgcccttgcc gggtcggtat agctcaatat aaggatgggc acgccgatgc ccattttcct
     3301 gatctggatg gcttcatcca gcatcgaaac ggccaactgg gtaacgccgt tgtcaagcag
     3361 cgtactgacc acctcaagca ccccgtgccc gtacgcgtca gcttttacga cgcccatcat
     3421 ctccacgttt ttgcccgtca gcttttttat ctccctggtg ttgtaggcaa tcgcgtccag
     3481 gtcgatctcc gcccatgccc tgttaagctt gtactccatg gtgtcccgtt tatataccat
     3541 catgtcccgt gtcctccgtc caacattctc caggtctctt tcaaaacctc tttgatcgtt
     3601 atcggcagta tgtccacaag gtcgcccgct accattccgt gcatcccctt tagttcggcg
     3661 gcagcgtcgc cggcaagccc gtgcaggtaa actcccgctg ctgcggcgtc gccgacaggc
     3721 actccctgcg cagcgatacc tgctatcatg ccggtgagca cgtcaccggt accggcagtg
     3781 gccatgcctg cattgcccgt ggtgtttatg agcacccggc cgtccgggta tgccactatg
     3841 gtcctgctgc ctttcagtac aacggccacg ccgtattgct gtgcgaaatt cagggctgtg
     3901 cccatcctgt ctgactgcac ctgtttcgta tttgttcccg tgatccttgc catttctccc
     3961 ggatgcggcg tcagtacagc atccgttttc agctttctta gcaccgacgt gtctgccgaa
     4021 atggcattga gcgcatctgc gtcgatcacg agcggtactt tgctgttttc gataactgcc
     4081 tcgacgatct tccttatatc atcgcccgcc gtcaggcccg gacctattgc tgccacatcc
     4141 attttatcca tcaggccgag gatgtgttca acgcatgagg ccgaaaggcg tccggtgccg
     4201 ttgtcttcaa gcggtatcac tatcggttcg gtcattttcg aggaatatac cggggcaaga
     4261 ctcttcggca cgcccgcgta cacaaggcct gcgccagtcc gcagcgccga catggatgca
     4321 aggcagccag agccggtcat gcccgtggag cccgtgatga tcagtaccct gccgtaatcg
     4381 cccttgttgc tgtcaggctt ccgccgcggg atcattgccg ataccatgtc aaggtcgatg
     4441 gtttcggtct ttatgtcctg cttctccagc acacaggccg gtatcccgat atcagccgtg
     4501 atcagctccc cggtgtaccg gcagcccgga tggagtatca ggcccagctt gggcaggcca
     4561 aaagtgacag tggcgtcagc tttgacgcac acacctttaa tgcttccgtc cctcccgtcg
     4621 atacctgacg gtatgtctat tgacagtaca ggccttccgg atgcgttgat gcgctcgatt
     4681 atctctgctg caaaccccgt gatatccctg ctgagacccg tgccgaacag tccgtccagt
     4741 atgaggtcgg acacggcaag gtccgacacg aaaccgtcga ggcctgtttc ttccgtaagt
     4801 tctgtaattc caaagccgat cctttcaagg atctccatgt tgaaaagcgc gtcgcctgac
     4861 aagcccttct tttcacccat aaggtacacc ctggcgtccg ctcctttgct gtggagcagc
     4921 ctgactgccg cgaatgcgtc gccgccgttg ttgcctctgc cggcgactac cgtaacgagc
     4981 ctgccggcgc agcctcccat catggccctg gcctgcgcgg ctactgccgc agcggcattc
     5041 tccataagca gtatgccggg gatcccgtac tcgtttaccg tgcattcatc gatttttttc
     5101 atttgcccgg gtgatacgac cttcatcact cagcctccat tcgcagctac tctatgggcg
     5161 cctgtctccg atcgtgatcg cggatttcga tcatgtttcc tgattcagat tatgttcccg
     5221 gacttcgacc ggcccttaaa tcatgctgat acggccgaat atgcggcact caacgtcgta
     5281 ctgccgatgc tcatcatcgc gcccctgctg ttgtttgaaa acatcgctcc gaatgccgtt
     5341 tgagaatatt gctccgaatt tcatctgaaa aacgccgcgc aacagccgca tcgcatccga
     5401 aagatgtccc gtatcacttg aaaaacatcc tgtctatctt ctcaagctcc tccccgccaa
     5461 gcaggtcatg gaagtacgaa tatatgtccg agtcatcctg cgacagcgct tccttctcgt
     5521 cgatatattc cttcagcttc gccttcgtct cttctatcag cttctccagt tcctcgactc
     5581 tttttctgcc cgaacgtatc ttataatata cgaccataac cgtcaactcc agaagttcga
     5641 tgtttgcatc cctgatcctt gacggtattt cttcaagttc ctcttctatc cgttttgctc
     5701 tcgcatttat cctttttatc tcctgttccg aggctttcat ctcatccctg gcggcttcgc
     5761 tgcccttttc gaaggcttcc gtcgtgagct ccatgatcct gctcaaatga tgcttcttgg
     5821 cagccgcgat ctccttctgc tctgccagaa ggcgggcttc ttccttcatg agttccctga
     5881 gcttctgttc acagcgttcg atctccggag ttttctgcgt attggtaaaa agcttgttcc
     5941 agcgctcgtc aaggatgagg agcgatatgt cgttccttct gagcgtacct gcgtcaaatt
     6001 tcagcttcat tctcatttgt cttaatttct tcgggaatat cttcggcagc ggcaatcgtc
     6061 atcacctccc ggttaaagca tttttcaggt atgacagcga ttcttcccgc tttttctgca
     6121 gtatttccct gaccgtcaga taatcgatgg cgccagtctg gccgcccagc agcttcctga
     6181 aaccgtcaga gcctgaagac agatgataca gtcgtgaaag gagccccgtg acctcaagta
     6241 tgttgtgctg tctcgaaccg acagcttttg aaggatccct ggaagcctcg aaggagtaga
     6301 attccttttc gaatataagg gagaaaactg tcccatggaa cgaatcggtc aggaccgccg
     6361 atgcccccgc gaacaggccc aggaactcgc gcggcccgca atcgagcctc ttgtattttg
     6421 tctgtggaac gacccttctg gaaccgcaga gagatacgac gggcaggccg tatttcccgg
     6481 cgaccgcccc gacatagtcc gcgtattttc tcgcatccgt gatgaaatag cagaggatgt
     6541 agccgccgcc tgcattttgg cggccgctgt ccgccttcgc agatttttct cctgtacccg
     6601 gatatgcgcc gcatgcttcc gcggccgacg cgattttgcc ccattcctcc gccggaagga
     6661 gcagtgtcgg gtcgagcacc gtgaccgctt cccgacccgt tatttccctg attatcttct
     6721 cgccctgctt ttcccgcgaa gaaaggctgt cgaatttctc aaggtaccgg gcaagctcct
     6781 cccgtttgtc ctccggtatc gaacttacgc cgaaactcgg ggcataggcc acacgcctgc
     6841 cgttcacggc aaaatcggca aagaacgccg gatcatatgt cttttcactg aaaatgaagg
     6901 ggttccatat ctggtcgctg ccgcatacat aaacgtcata cgcgggcgga tcatcgcaaa
     6961 gctctttata tgagccgtag aatttaccgg aaagtctcat ttcatcggca acaaaactgt
     7021 cgaaccggtc gtgccttttt ttgaggctgc cgtaatgcag cagtgtgtcc gcatttgaaa
     7081 ggatagatgc cggcgacagg ccttttttga aaaggctgct tccctctatg ttgtccttcc
     7141 tgacatagtt gattatctcg caatccacgc caagaagctg tattgcccgc attaacgcgt
     7201 atgcctgcaa ctgggccccg taatggtgcg catagtggaa tgtcaatata cctgctttca
     7261 ttctgttgat ccctccgtca gccggcaacg gcctttaccc gttttccggc cgattcacaa
     7321 atcctgcatg aatcttccgc cggccttttc atttcccagc ccggttcctg gccagctgtc
     7381 ttactctttc ataaactgca gctgccgttt ttctctccat cagtatgttt cctgcgccga
     7441 tgaccagcag cgcccatatc ccgacggcca ccgcatataa cgcccattcg atatagcccc
     7501 cggtattaat atcggcaaaa tataccgccg ggagcactgt tataaagaac agcacgacaa
     7561 gcctgatcac ccttcttatc gtcatgaacg ggctcagctt cgtcactgtt ttgggtatgt
     7621 agaaaaacag gtcgatgtca cggtagagat tcgatagtat ggcgccggtc aatacaccca
     7681 cgataccgaa gtacggcgcc agcaaaactg aggccacgac tattatcagc gcctgtataa
     7741 gcgactgaag cctcgtttcc ctgtacagtc ccgccgatat aacgagcatg ccctgcgggt
     7801 tctttatgtt cgttattatc ccggtcgcca cgaacattgc ggctaccgtc ggcctaatgt
     7861 attcggcgtc ggtaaacccg gccgtatata tctccatgaa cggcatgatc atcaccgccg
     7921 cgacggagta accccacgcc accagcgcat aatacaggta ttcgtactgg tggtacgcct
     7981 tctgcagcgt cggcagttcg ttcctggcca gcatgtcgcc gaatatggcg ctaagcccgt
     8041 tgctgaacgt ggtcagcagc gccagcacgc ttgtgaacac catgttgtag gtgacaaata
     8101 cgcttgcctc gctgaggctg acaaaaaggg acgtgacgac aacaggcgtg gcagtcgatg
     8161 ccgctccgag tatctggagc accagggcgt cccacctctt gtccagcgcc tcgtttttgg
     8221 gcggcgcgct gaagtctatg tctttgtact tcagcctgcc gtagatgaca taaagcgccg
     8281 aacgggcgaa gaagctggtc agcgctgcca gctggacgat cactatattc agccggaagc
     8341 ttgcaaggat cagaatgatc actgcgttca tagccacggc cgccgcattt atcagcgaga
     8401 tcacgtaact tttctggtcc gccgtgtaca gcactttgta tttgcccacc gcaaagaact
     8461 ccatggcgcc ggacgcgccg attatcagca caagggcggc aatggtgaac acatctatgc
     8521 cctggccgga tgtgagcatc gggtagatga aagcggacac gaccacaagc gccgagaaaa
     8581 tatagccgga gatgttgtaa aaccggttcg acgcggacag tatcccgttt atgtcgtcgc
     8641 tgttcttctc ggccaggggc ttgtacagcg catagaccgc agccccggcc aggccggctt
     8701 ccacgaggtt gaaataggaa atgaactgcc ttatggatgt cacaaggccg ttcacatttg
     8761 agccgtatac cgttatcatg accctgggca ggatcaggtt tactataatg tttacgagct
     8821 gaagcgccac ggcgcttatc gagttgtaaa aggacagctt tgtccgcatg atctactatc
     8881 aacccctgcc taaaactttc tttatgatgc acttaacctg gtacttgatg cttgtgttgt
     8941 ccatatactt tcttctcagc ctgtcgaagc tgtggccgtt tttcatatct gcgaaaaacc
     9001 tggcccttcc cgccggctcc ggcaatgatg aaacaagcct cgggttgtac ctgacagcgt
     9061 cctccggggc atattcgacg atatccgcac ggtcactgat gccgaacagt gcttcttctc
     9121 cggcctttgt gtgtgtaata acgagcgata tacccctgtt gtcatccctg accgccgtgt
     9181 catcttctct ggctctagtg tcaccggcct cgggcaatgt gtcaccgtct atggtagcaa
     9241 tatcatcgcc cttgaccgtc ctgtcgctgt ccatgagcat tgtgccacca tctgtggccg
     9301 cagggccttt tcgttttgcc aggcctttta atttctcaat gccccagaaa tcggccagcg
     9361 ttatgtcggc ctgtgagttt tggatcctga acctgcagtc gaaacaggac ctcctggtat
     9421 acaggttttt gaaaaaaccc atcaggtaga gttctttttt gacacttacc acgtacctgc
     9481 cgccgttttc aaatccgatg ctgacgctgg attccctcca tcctccggat ttgtcgcgga
     9541 aagatacgga cgaaatgcgg ctgttatacc tttttcccag gtaatcgaca aacatcctcc
     9601 agaccttcgg ggacggcacg ccgaagcatg cgaggtcgca cgccgtcagg ccggcatact
     9661 tcttccccag gtaagcctta agcccagcga tctggcacgg tgtcccgcag aacagtactt
     9721 ttctgccttc tttcaggtat ttttcagctt ctttgaaagt ttcgccgatc tcgctctgca
     9781 catatttcga ccggcgcaga tcgtccagtt cgccgatgct ttctatacat ttatgcttta
     9841 cattgaattc gctgtcaaac gcagcgccga acacggctcc gccgtcttcc agcgtttttg
     9901 cggcaagctc ggaaaatatg ccgccggacg agcttttttc ccttgtcccg tcgtttttcg
     9961 aacaacaggc aaaggctctc attccgccga aatttgacgc ggagccgtca gtcgattttc
    10021 tgcagccgcc atcggtttca tcccggacag attccgccgc actgttttct ctgcttccaa
    10081 acaccggcac actgcttctg cctgcggtaa aaacggacgt gccatccgtg tcagtaacga
    10141 tactgcccgt tttctcaaac gcggcattga ccgggcatgt cctcctgcac aggccacagt
    10201 cattgcaccg ctctccattt attttcggaa aaatgaaacc ctcgtcatcc gcttccatcg
    10261 ccacggcgcc cctggggcat acctggctgc aggccgaaca ccccgtgcag taccggatgt
    10321 tgtagattat ctgctccata cctctttaac ctctgcctct cttcgatatg tttattataa
    10381 caccttattc gaaggaattt tttcgcttgt agaaaaaatt cctcacctta gcggtgtttc
    10441 aacgaagcga agtctcgtta taacgcctta ttcaaagtaa tttttcggtt gtagaaaaat
    10501 tactcacctt agcggcgttt caacgagacg aagtctcgtt ataacaccta tttcagagta
    10561 attttttcgc ttgtagaaaa aattcctcac cgcagcggcg tttcaacgag acgaagtctc
    10621 gttataacac ctaattcgaa ggaattttaa taaaagtgcc aggctgtttt ttgtgtatcg
    10681 caaaccgcct ggcacttatt atatggtttt cccggcagac gttctctttg cctgccggct
    10741 tccttactcc ttc
//
LOCUS       DAOSCC010000067        10734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4060_length_10734_cov_4.292069, whole genome shotgun sequence.
ACCESSION   DAOSCC010000067 DAOSCC010000000
VERSION     DAOSCC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_10734_cov_4.292069"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLG72_05135"
     CDS             <1..201
                     /locus_tag="PLG72_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792380.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ80215.1"
                     /translation="VRPERRQALGLRWLVGFARERSEKTMKERLAAEIMDAANSLGGA
                     FKKKEDTHKMAEANRAFAHYRW"
     gene            319..2391
                     /gene="fusA"
                     /locus_tag="PLG72_05140"
     CDS             319..2391
                     /gene="fusA"
                     /locus_tag="PLG72_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ80216.1"
                     /translation="MPRQFSLENTRNIGIMAHIDAGKTTTTERILFYTGKVHRMGEVH
                     EGAATMDWMEQEQERGITITSAATTCQWKDTRINIIDTPGHVDFTVEVERSLRVLDGS
                     VTVFCAKGGVEPQSETVWRQADKYHVPRIAYVNKMDILGADFFNCIQMMKDRLKANAV
                     PIQLPIGKEENFKGIIDLVTMKAIYYLDDLGTVLDEEPIPEDMIEIAEEYRTNLLEAV
                     AEQDEDLMEKYLSGEELTEEEIHAGIRKATIAVQMIPVTCGSSYKNKGVQKLLDAVVR
                     YLPSPVDIPPITGVSPDDPEKEVIRTADDDAPFSALAFKIMTDPYVGKLCFLRVYSGK
                     LNAGSYVYNSSKGRKERIGRLLLMHANHREDIDTAYSGDIVAVVGLKDTSTGDTLCSE
                     ETPVILESMDFPEPVIRVAVEPKTKAGQEKMAVALAKLAEEDPTFKAYTDPETGQTII
                     AGMGELHLEIIVDRMLREFKVEANVGQPQVSYKETIRKAVKSEGKFVRQSGGRGQYGH
                     VVIEIEPQEPGSGYTFVNKIVGGVVPKEYIPAIDAGIQDAMQNGVLGGYQVLDVKVTL
                     VDGSYHEVDSSEMAFRIAASMAFKDGCRKADPVLLEPIMKVDIVIPEVYMGDVMGDLT
                     SRRGRIEGMVERSGAQVITAKVPLANMFGYATELRSRTQGRGVFTMQFSHYEEVPKNI
                     QDSVLKIG"
     gene            2543..3745
                     /gene="tuf"
                     /locus_tag="PLG72_05145"
     CDS             2543..3745
                     /gene="tuf"
                     /locus_tag="PLG72_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ80217.1"
                     /translation="MGKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLGFQGKAQF
                     TAYDQIDKAPEEKARGITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKCDMVDDEELIELVEMEVRELLSQ
                     YEFDGDNIPIVRGSALKALESASTDPSAPEYQCILELMNTVDEYIPEPQRPKDKPFLM
                     PVEDVFSITGRGTVATGRVERGTIKVGDEVEIVGLMDEARKTVVTGVEMFRKLLDEAV
                     AGDNIGCLLRGIQRNEVERGQVLAKPGSIKPHTHFKGQVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGVAELPEGVEMCMPGDHVEITVKLITPIAMEEGLRFAIREGGRTVGS
                     GTVTAIIE"
     gene            complement(3857..4831)
                     /locus_tag="PLG72_05150"
     CDS             complement(3857..4831)
                     /locus_tag="PLG72_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80218.1"
                     /translation="MYLFLKSIYEKAYNDPSLLKLKAIPDDSYSDWEFHKAKPELITE
                     IRDNIKKVEQLIFLLFQICLSKQLDENSFFVPETDMEIKPSALKQLRNFDIQCQASGD
                     GYTFTFPVKAAGLKLLAETSMQNRGNGIDIPKPHMLFSRGVFDPNHPWTYEVFRNLSK
                     EWNAFDRLISFLDRNDYERIDCSEGFSLNYVKNYGKKREKLKAAWAERTHGGIELVYE
                     EARRNPVLLSLRIPYFKEILEHSDKMDDKLKSFITGTTKKCDGCRYCVQTDKSGTRPL
                     AAINVDDHDLCPLFCGFSYRWRTIDDDLVDNIIELLQFIDEAFRERCA"
     gene            5702..6655
                     /locus_tag="PLG72_05155"
     CDS             5702..6655
                     /locus_tag="PLG72_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ80219.1"
                     /translation="MELNDVRKLAVAVKENISKVLIGIEDTVDLVLISLLCGGHVLLE
                     DVPGVGKTMLAKCLSRSLECGFKRIQFTPDLLPSDLTGINYFDQKRGEFIFRAGPLFT
                     NIVLADEINRATPRTQSSLLECMEERQVTVDGETRKLHSLFFVIATQNPVETRGTFPL
                     PEAQLDRFFMRLRMGYPDPSEGMEILLRFRDKDPLAELDTVASADSVIQAQKAVTSVK
                     VSDASARYIIDIIEATRHNERITLGVSPRGSIALMKAAQVKAVLEGRDFITPDDVKQM
                     AVPVLAHRIILKSHAVFSGTENAEETVRDILNRVPVPTEEI"
     gene            6655..7803
                     /locus_tag="PLG72_05160"
     CDS             6655..7803
                     /locus_tag="PLG72_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ80220.1"
                     /translation="MDVIALLLILAFVLWLQLQILCRFVFSRLEYKCEFSVPEAHEGD
                     TIMLVETVYNGKLMPVPWLRVDIHTSRWLEFAGTCSVIAQDNRHVTSSFVLKSYQKIT
                     RKWKLKCIKRGIFQTENVTLVSGDLLNFTTVSIAVPVNATLTVYPQIIDLEELFVPVS
                     LQQSDRVVNRWIIDDPFLVAGVRDYTYGDPFNRMHWPASAKNGRLMVRKNEYTTQQNL
                     TVILNMQSRHYELLDTVDKAIAELGIKVAATLFDRALRNGNPVRFITNGCTDPDAKQP
                     IVTGEAADRDHITSLLRILAGLKMKNIKDFRVLLEDTSSDIYNSEVVIITAYLTVEIS
                     RLADRMASEGNDVHVVLLDSVYERGSEPRHADLYVLSDRIRAEAEKLA"
     gene            7863..9392
                     /locus_tag="PLG72_05165"
     CDS             7863..9392
                     /locus_tag="PLG72_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOJ80221.1"
                     /translation="MKSTARDKNPRVIDTSYATENTSKSEKREYRKSRAGRRVRPSFR
                     TALLRLLSSLAVNIYIYALVTAIRTILFYDDGGAASVLVYTCGFLTAYLSGCLLAGRS
                     TYIAGKLSRTKVFRFIAYALGIRRMDQAIASLAAWLVIIVPAALPFAIFRGDGILRPL
                     LESAATCCAYIVSFMHTRMEPADIIGGGGFYAGLLILGGCLELHYMADVITYLRPWFF
                     RALYFLIFVYLVVRNQDDIDDNIYDKKYVEKSILPKNLRRVNTVSAIFIFLVLMFLSN
                     FKTVVSGLVRLAGRFVRTIIIAVVSLLEKITPDTGGDSGMSGPEELAPQFGEAPPSPY
                     LDLVFNILKYFIVLYLSYKLVCLIISKIPAIVTALAKLLRKLFRIRTDKQDYEGSDYI
                     DEAEIVLPERAERDGRRARKSKRRTARQLRKIKDPVERVRYMYGLILDTLPLRGIKPL
                     PSDTTAEILAKAGGTDGLHPFTAIYDHVRYGGMVPDKETLAKAEAHYEKAYDTISGRF
                     M"
     gene            9550..>10734
                     /locus_tag="PLG72_05170"
     CDS             9550..>10734
                     /locus_tag="PLG72_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOJ80222.1"
                     /translation="MPGSRKTIKKRILPMLPLRGLTVFPHMILTFDVGREKSINALDE
                     AMVNNQLIFLTTQKDAKNDSPDVDDIYNVGTISRVKQLLRLPGNTIRVLVEGLNRAEI
                     LKYIKTEPFFIIEAAERIIPENREKSIIDEALKRKVTSTFEDYAKLSGKITPETVSSI
                     SAIEDIGQLSDVIASNMHLRIEQKQVILDEFDPEKRMEKLLELLMSEIEILEVEKDIS
                     IRVRKQIDKMQREYYLREQIKAIQSELGDKDGITGEVEEYRKKLETIKLPEEVEKKVL
                     KELDRLQKMQSGSAEGSVIRTYLDWIFDLPWGKKTEVMIDLKKAEAILEEDHYGLEKV
                     KERILEYLAVLKLTNNLKGPILCLVGPPGVGKTSIAKSIARALNRNYVRMSLGGVRDE
                     AEI"
BASE COUNT         2972 a         2436 c         2918 g         2408 t
ORIGIN      
        1 gtaagacctg aaagaaggca ggctctggga ctcagatggc tggtcggttt cgcccgtgag
       61 agaagcgaaa agaccatgaa ggaaagactt gccgcagaga tcatggatgc tgccaacagc
      121 cttggcggtg ccttcaagaa gaaggaagac actcacaaga tggcagaagc caacagggcg
      181 tttgcacatt atcgctggta atattattgg tcctgatgca gcataggcag tatataaaga
      241 ggatcaataa gcaaaactta tagaaacagg atattccgta tatcggatca gaactaatcg
      301 agaagaaagg aggcaatcat gcccaggcag ttcagtttgg aaaataccag gaacattggt
      361 ataatggcgc atatagatgc tggtaagacg actaccactg agcgtatcct gttctataca
      421 gggaaggtgc acagaatggg ggaagtccat gaaggcgccg ccacaatgga ctggatggaa
      481 caggagcagg aaagaggcat caccattacc tctgctgcta ccacctgcca gtggaaggat
      541 accaggataa acattataga tacgccgggg cacgttgact ttactgtaga ggtcgaaagg
      601 tctctgagag ttctcgatgg ttcggtaacg gttttttgtg caaagggcgg agtggagccc
      661 cagtccgaaa ctgtatggag acaggcggac aagtaccatg ttccgcgaat tgcatatgta
      721 aataaaatgg atattttggg tgcagacttt ttcaactgca tccagatgat gaaggacagg
      781 ctgaaagcaa atgctgtccc gatccagctg cctatcggca aggaagagaa tttcaaagga
      841 ataatcgacc ttgtgaccat gaaggccata tattatttgg acgatctggg aaccgtgctc
      901 gacgaagagc cgatccctga agacatgatc gagatcgctg aagaatatcg aaccaattta
      961 cttgaagctg ttgctgaaca agacgaggac ttgatggaga aatacctgtc aggagaagag
     1021 ctcacggagg aagagataca tgcggggatc aggaaggcaa ccattgccgt tcagatgatc
     1081 ccggtcacat gtggttcgtc ctacaagaac aaaggtgtcc agaaactgtt ggatgctgta
     1141 gtgcggtatc tgccgtcgcc ggtggacata cctccgataa cgggggtttc ccctgatgac
     1201 ccggaaaagg aggtcataag gaccgctgat gacgacgcgc cgttttctgc gcttgcattc
     1261 aaaataatga cggatcctta tgtcggaaag ctctgttttt tgagggttta ctcaggcaag
     1321 ctcaatgcag gctcatatgt gtataactcc tcaaaaggca ggaaggagag gatcggcagg
     1381 ctcctgctga tgcatgcgaa ccacagggaa gatatcgata ccgcttattc gggtgatatc
     1441 gtggcagtcg tggggctcaa ggacacgtct accggggaca ccctttgcag cgaggaaact
     1501 ccggtaatac tggagtcgat ggatttcccc gaaccggtca tccgtgttgc cgtggagccg
     1561 aagacaaagg cagggcagga aaagatggcc gtcgcactgg cgaagcttgc tgaggaagac
     1621 ccgacattca aggcttatac cgatcctgaa acaggtcaga ccatcattgc cggaatgggt
     1681 gagctgcacc ttgagatcat agtcgacagg atgctgaggg agttcaaggt cgaagccaat
     1741 gtcggacagc cgcaggtgtc atacaaggag accatccgca aggctgtcaa atccgaaggc
     1801 aagtttgtaa ggcagtcggg cggcagaggc cagtacggcc atgtcgttat cgagatagaa
     1861 ccccaggagc cgggaagcgg ctatacgttc gtaaacaaga tagtcggggg tgtcgtgccc
     1921 aaggaatata tcccggctat cgacgcgggt atccaggatg ccatgcagaa cggtgtcctt
     1981 ggcggctacc aggtgcttga cgtcaaagtc actctcgttg acggttcata tcatgaagtg
     2041 gattcatccg agatggcctt caggatagca gcttcgatgg cattcaagga cggctgcagg
     2101 aaagcggatc ccgtgcttct tgaacccata atgaaagtgg acatcgttat acccgaggtg
     2161 tacatggggg atgtcatggg cgatcttacg tcacgccgcg gcaggataga aggcatggtc
     2221 gaaagatcgg gagcacaggt gataacggca aaagtaccgc tggccaacat gttcggttat
     2281 gcgacagaac tccgttcaag gacacagggc agaggcgtct ttacgatgca gttcagccac
     2341 tatgaagagg tcccgaagaa catccaggat tctgtgctga agataggctg aagtgatgcg
     2401 ggccacgcag catcggccta taggctggtc aatatgccgc aaaagcagac aagcccagaa
     2461 aatgtttgcg gcatgtgaaa taaaattata aaattatata aaaacatttc agtatttcac
     2521 tgaacaaggg aggagaatca aaatgggcaa agccaaattt gaaagaacaa aaccccacgt
     2581 taacattgga accatcggtc acgtcgacca tggcaagact acattgacag cagccatcac
     2641 gaaggttctg ggattccagg gcaaggcaca gttcacggca tatgatcaga tcgacaaggc
     2701 tcctgaagaa aaagcaagag gaatcacgat atcgaccgca cacgttgagt atgagactga
     2761 gaagaggcac tatgcacacg ttgactgccc cggtcacgct gactatgtca agaacatgat
     2821 caccggtgca gcccagatgg acggcgccat cctcgttgtt tccgcagctg acggcccgat
     2881 gccgcagacc agggagcaca tactccttgc tcgtcaggtc ggcgttccct acatcgtagt
     2941 attcctcaac aaatgcgata tggtcgacga cgaggagctt atcgagctcg tcgagatgga
     3001 agtaagagag cttctcagcc agtatgaatt cgacggagac aacattccga tagtaagagg
     3061 atccgctctg aaggctctcg agagcgcttc gaccgatccg agcgctcccg aataccagtg
     3121 catacttgag ctcatgaata ctgtcgacga gtacatcccc gagcctcaga ggcccaagga
     3181 caaaccgttc cttatgcctg tcgaggacgt attctcgatc accggccgtg gtacggttgc
     3241 tacgggcaga gttgagagag gtactatcaa agtcggtgac gaagtcgaga tcgttggtct
     3301 catggacgaa gcaagaaaga ccgtcgttac cggtgtcgag atgttcagga agctccttga
     3361 cgaggctgtt gccggcgaca acatcggctg cctgctcagg ggtatccaga ggaacgaagt
     3421 cgaaagggga caggttcttg ctaagcccgg ttccatcaaa cctcacaccc atttcaaggg
     3481 ccaggtctac gttctgacca aggaagaagg cggaagacat accccgttct tcaacggcta
     3541 caggcctcag ttctacttca ggaccaccga cgttaccggt gttgctgaac ttccggaagg
     3601 tgtcgagatg tgcatgcctg gtgaccacgt agagatcacc gtcaagctga tcactccgat
     3661 agccatggaa gaaggactca ggttcgctat ccgtgaaggc ggcagaaccg ttggttccgg
     3721 taccgttacc gctatcatcg agtaataatg taaggggaca ctcctctgaa gcgggcagca
     3781 atctccggca gaggaatgtc ccctctcgtt cagagagaca gaagggacac gtaagtgtcc
     3841 cttatctgct tttctgtcac gcgcagcgtt cgcggaaagc ttcgtcaatg aactggagca
     3901 gttcgattat attgtcaacg aggtcgtcat cgatcgttct ccatctgtat gagaaaccgc
     3961 aaaatagcgg acataggtca tgatcatcca cgtttatggc tgcaaggggc cgtgtgcctg
     4021 atttatccgt ttgcacgcag tacctgcagc cgtcgcattt ttttgttgtg ccggtaatga
     4081 agctcttaag cttatcatcc attttatcgc tatgttccag gatttcctta aaatatggta
     4141 tccgtaagga caggaggacc gggtttcgtc ttgcttcttc ataaaccagt tctatgcctc
     4201 catgggtacg ctcagcccat gcagctttta gcttctcccg cttttttccg tagtttttca
     4261 catagttgag ggaaaaacct tcgctgcagt cgatcctttc ataatcattc ctgtccagga
     4321 aggatatcag cctgtcaaat gcgttccatt ctttggacag attccggaat acctcgtatg
     4381 tccagggatg gttcggatca aatacgccgc gggaaaacaa catgtgaggc ttcggaatgt
     4441 cgatcccgtt tcccctgttt tgcatggatg tttccgcgag cagcttcagc ccggctgctt
     4501 ttaccgggaa tgtgaatgtg tatccgtccc cggatgcctg gcactgtata tcaaaattcc
     4561 tgagctgttt cagagcggaa ggttttattt ccatatccgt ttcaggtacg aagaatgagt
     4621 tttcatccag ctgttttgac aggcatatct gaaagagcag gaagattaac tgctctactt
     4681 tttttatatt atcacggatt tccgtaatca gctcaggttt tgccttatgg aactcccaat
     4741 cagaataact gtcgtcggga attgccttta acttcagcag ggaagggtca ttatatgctt
     4801 tttcatatat gctttttaaa aaaaggtaca ccgtttcctg ctcttctgcg gttgctgcat
     4861 cggacctgac aggaatgaac ggcgggtatg tggcgagaaa atagttgagg cttctttacg
     4921 caaggttgtc aaatgatttg ctttccgtgt tattcatatg cccgtgtcct ttcgtgatta
     4981 ttctattacc atttattagt tatgtgtgac attataaaat aaatttgtcc ataatccaac
     5041 aaattttgac ttaaaaaacc tggggcattc ccggatcctg ctttgtcatg tacaaggata
     5101 tgaactgtac gggaaagcgc tgaaggtgat aagtgcgtca ctcgaaaaat caggcctgaa
     5161 aattcgatat ggccgatgga gcaaaataca gaacgccggg aacagaaccg gaggaatgtg
     5221 ccgctttcgg ggaaaatttc gcaaaggcaa cgtaagaatg aagccgttta tcttgtttta
     5281 actacttcca gatgataaca ttaaaaagaa aggggcagag caaggcagga cggggggatc
     5341 atgttgccgc tgttcaggcg tataaaatcc gctttaagaa acatggagcc agcaaccata
     5401 agagaaatta ttatcgtact ggtcatactg atcatatcca tagtactcag cataccccag
     5461 tttcttgccg acatggaagc tttgcgcaga tgggagcagg ttcaccggaa caccggaagc
     5521 tatacagacc cagttgggaa ttgaacctgc tattgggagt cgtgcgggtg aattccatga
     5581 agacataccg cggatcaaac ccggtgaatg cagttgacag gccgggatat acagaacgct
     5641 gcagaacgga aagcccggta tgagtttaaa agcttataaa tcagaacatg gaggtcgtga
     5701 aatggaactg aatgacgtaa gaaaactggc agttgccgtg aaggaaaata tatcgaaagt
     5761 cctgataggc atcgaagaca cggtggacct tgtcctcatc tcgctgcttt gcggcggaca
     5821 tgtactgctg gaggacgttc ccggcgtcgg aaagactatg ctggcaaaat gcctttccag
     5881 gtcgctggaa tgcggattca agaggataca gttcactcct gacctgcttc cgtccgacct
     5941 tacagggatc aattacttcg accagaaacg gggagaattc atattcaggg cgggaccctt
     6001 gtttacgaac atcgtgcttg ccgatgagat caacagggca acgccgagga cacagtcgag
     6061 tctgctcgaa tgcatggagg aaaggcaggt aaccgtcgac ggtgagaccc ggaagcttca
     6121 cagcctgttc ttcgtcatag caacgcagaa cccggtagag acaaggggca cttttccgct
     6181 gccggaagcg cagcttgaca ggtttttcat gcgcctgagg atggggtacc cggatccgtc
     6241 cgagggcatg gagatactgc ttcggttcag ggacaaggat ccgctggcgg aacttgatac
     6301 cgtagcttct gcggactccg tcatacaggc gcagaaggct gtgacatccg taaaagtatc
     6361 tgacgcatcg gccagatata taatagatat aatcgaagca acaagacata atgagagaat
     6421 aacgcttgga gtgagcccaa ggggcagtat agcgctgatg aaggcggcac aggtaaaggc
     6481 ggtgctggaa gggcgcgatt tcataacgcc tgatgatgta aagcagatgg ctgtacctgt
     6541 attggcccac cgtattatat tgaaaagcca tgctgttttc agcggcaccg agaatgcgga
     6601 agagactgtc cgcgacattt tgaacagggt ccctgtgccg acggaggaaa tctgatggat
     6661 gtaatagctt tgcttcttat cctcgccttt gtgctttggc tccagctgca gatcctgtgc
     6721 agattcgtgt tcagcagact ggaatataag tgtgagttca gcgtgcctga agcacacgaa
     6781 ggcgacacca taatgctggt ggaaaccgtt tacaatggaa aactgatgcc ggtgccctgg
     6841 ctcagggtgg acatacatac gtcaaggtgg ctggaatttg cagggacctg ctccgtgatt
     6901 gcccaggaca acaggcatgt gaccagcagt ttcgtactga aaagttatca gaaaattaca
     6961 cgcaaatgga agcttaaatg cattaaaagg gggattttcc aaactgaaaa cgtgacgctg
     7021 gtaagcggcg atcttctgaa tttcactacc gtttccatag ccgtgcctgt caatgccact
     7081 cttacggtat atccacaaat catagacctg gaagaactgt ttgttccggt cagcctgcag
     7141 cagagcgaca gggtcgtgaa cagatggatc atagatgatc cgttccttgt ggccggggtg
     7201 agggattata cctatggcga tccgttcaac cgcatgcatt ggccggcttc tgcaaaaaac
     7261 ggaaggctga tggtcagaaa aaatgagtat actacgcagc agaaccttac cgtgatcctg
     7321 aacatgcaat cccggcatta cgagctttta gacacggtgg acaaagctat tgcggaactg
     7381 ggcataaagg tcgctgccac attattcgac cgggctctca ggaatgggaa ccctgtcagg
     7441 ttcattacca acggatgtac agatcccgat gcaaaacagc cgatagtgac cggcgaggca
     7501 gcagacaggg accatataac ttcgctcctc aggatcctcg caggtctcaa aatgaagaat
     7561 ataaaagatt tcagagtact cctggaagat acatcatcag atatatacaa ctccgaggtc
     7621 gtcatcatca cagcttatct caccgtggag ataagcaggc tggctgaccg gatggcgtcc
     7681 gagggaaacg atgtgcatgt agttttgctc gattcagtat atgaaagggg ttcggagcca
     7741 agacatgcag atctgtatgt attatccgac cgcatacggg cagaggctga gaaactggcc
     7801 tgaacggaac gacgtgctga acaggccgga cggaacggca ttctgacaga aagtaaaaaa
     7861 gtatgaaaag cacagcaagg gacaaaaatc cgagggtgat tgatacgagt tacgcgacag
     7921 agaacacatc caaatccgaa aagcgtgaat atcgcaaaag cagagcggga agaagagtaa
     7981 ggccgagctt cagaacggca ttgctgcgcc tgttgtcgtc gcttgcagtg aacatatata
     8041 tatatgctct tgtcaccgcc atacggacga tccttttcta tgatgacggc ggcgccgcgt
     8101 ccgttctcgt atatacatgc ggctttctga ccgcttacct gtccgggtgc ctgctggccg
     8161 gcagatccac atatattgca ggaaaattgt cccgtacgaa agtgttcagg tttattgcat
     8221 atgcgcttgg catcaggaga atggaccagg ccatagcttc actggccgca tggcttgtca
     8281 taatcgtgcc tgcggcattg ccgttcgcca ttttcagagg tgacgggatc ctgaggccgt
     8341 tgctcgaatc agcggcgaca tgctgtgcat atatcgtttc attcatgcat acacggatgg
     8401 agccggccga tataataggc ggaggcggct tctacgcagg attgctgatc ctcgggggat
     8461 gcctggaact gcattacatg gcggatgtga tcacatatct tcgcccatgg tttttcaggg
     8521 cgctgtattt tctgatcttt gtgtacctgg tagtcaggaa ccaggatgat atagacgata
     8581 atatttatga caaaaagtac gtggaaaagt cgatactgcc gaagaacctc aggcgtgtca
     8641 atactgtttc agcaatattc atatttcttg tgttaatgtt cctgtcgaat ttcaaaacgg
     8701 tggtttccgg tctggtccgt ctggccggac gatttgtccg caccatcatc atcgcagtcg
     8761 tcagtctcct ggaaaagatc acgccggata ccggaggaga cagcggcatg tccggacccg
     8821 aggaactggc gccccaattt ggagaagcac cgccgtcgcc atacctggac ctggttttca
     8881 acatacttaa gtattttatc gtactctacc tgtcatacaa actggtctgc ctcatcatct
     8941 cgaagatccc ggcaattgtc acggcactgg cgaagttgct cagaaaactg ttcaggatca
     9001 gaacggacaa acaggactat gagggatccg attacatcga tgaggccgag atcgtcctgc
     9061 ccgaaagagc ggaaagggat ggacgcaggg cccggaagag caagcggagg accgccaggc
     9121 agctcaggaa aatcaaggat cctgtggagc gggtcaggta catgtacggc ctgatactgg
     9181 atacgcttcc cctgcgcggt ataaaaccgc tcccgagcga cactacggca gagatacttg
     9241 caaaagccgg cgggacggac gggctgcatc cttttaccgc gatatatgac catgtgcgct
     9301 atggcggcat ggtgccggac aaagaaacgc tggcaaaggc tgaggcccat tacgaaaagg
     9361 cgtacgatac gataagcggc agattcatgt gacaggcggt accggtatgg ccttcatggc
     9421 attgcaggac cgcatacata tgtcttgtta agtttaactg atataccata taaaacaatt
     9481 gaaaaagcag catgtacagg atataataaa gaccattgca aattgtttag ttagtaaggg
     9541 gatggtcaaa tgcctggatc aagaaagacg ataaagaaga ggatcctgcc gatgctgccg
     9601 ctcagagggc tgacagtgtt tcctcacatg atcctgacct tcgacgtcgg aagggaaaag
     9661 tccatcaacg ccctggacga ggcgatggtt aacaaccagc ttattttcct gacgacacag
     9721 aaggatgcaa agaatgattc gccagatgtg gatgatatat ataatgtagg aacgatttca
     9781 agggtaaaac agctcctgag gcttcccggc aacacgataa gggtgctggt ggaaggcctg
     9841 aacagggctg agatcttgaa atatataaag accgaaccat tcttcataat tgaggccgcg
     9901 gaaaggatca ttcctgagaa cagggaaaag agcataattg acgaggcgct gaagaggaag
     9961 gtaacaagca cctttgaaga ctatgcgaaa ctgagcggga aaatcactcc tgaaacggtt
    10021 tcttctattt cggcgatcga agacataggg cagctttcag acgtcatagc gtcgaacatg
    10081 catctccgga tagagcaaaa acaggtaata ctcgacgaat tcgacccgga aaaaaggatg
    10141 gaaaagctcc tggaactgct gatgagcgaa atagagatac tcgaggtcga aaaggacatc
    10201 agtatccggg tccgcaagca gatagacaaa atgcagcgcg aatattacct aagggaacag
    10261 atcaaggcaa tacagtccga gttgggagac aaggacggca ttaccggcga agtggaggag
    10321 tacaggaaaa aactcgagac gatcaagctt cccgaagaag tggagaagaa ggtcctgaag
    10381 gaactcgaca ggctgcagaa gatgcagtcg ggttctgccg agggttcggt catcaggact
    10441 tacctggact ggatattcga tctgccgtgg gggaaaaaga ctgaagtaat gatagacctg
    10501 aagaaggctg aagctatact cgaggaagac cactatggcc ttgagaaggt caaggaaagg
    10561 atattggagt accttgcggt acttaaactg acgaacaacc taaaaggtcc catactctgc
    10621 cttgtaggcc ctcccggggt cggaaagaca tcaattgcaa agtcgatagc aagggcgctc
    10681 aacaggaact atgtcaggat gtcgctggga ggcgtgaggg acgaagccga aatc
//
LOCUS       DAOSCC010000068        10706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4072_length_10706_cov_4.724345, whole genome shotgun sequence.
ACCESSION   DAOSCC010000068 DAOSCC010000000
VERSION     DAOSCC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4072_length_10706_cov_4.724345"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLG72_05175"
     CDS             complement(<1..313)
                     /locus_tag="PLG72_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOJ80223.1"
                     /translation="MSRDMFDEKNIVPMAPVSSEPFNSNDYIYEIKFDGMRCIGYYDN
                     GVVLRNRANKDITALFPEFRNIYKQFRHKCIIDSEIVVLNRGKPDYSRLNKRRFLSNI
                     MH"
     gene            complement(310..1278)
                     /locus_tag="PLG72_05180"
     CDS             complement(310..1278)
                     /locus_tag="PLG72_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOJ80224.1"
                     /translation="MKTSWKGSISFGMVYFPVNVYTATSGSVGVKLNYLHRKCNSRIR
                     YRKFCDACNEEVSQDDIVKGYEYEKDKYVVIADEDFEKLPIKSKKVISISHFVDEDEV
                     DPIYLDKAYFISPGNFSASKAFELFRRAMRETGKVGIAKISLTSNEYVAMIKPYDKGG
                     MLLYLLYYSDEIKKTEEITELNYNVDIHDNELKMAISLIDNMTGEFDIGQYRNEYQDA
                     LKQLIQSKIEGKEVVSPPEAQSNIINLMEALKASVEAAKHGRTDSKKKSRGKSTGKST
                     SNRIRKTATEDRNPAVGENGAGEPADVNAGADTSAKSKPGGKRKIL"
     gene            complement(1705..2715)
                     /locus_tag="PLG72_05185"
     CDS             complement(1705..2715)
                     /locus_tag="PLG72_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ80225.1"
                     /translation="MPVNIYDIAKKAGVSVVTVSKVLNNHPGVREYNRKKVLAAIEEL
                     DYKPNAAARTLARGKTGMIGIIVPCIDDAFMVQVMQSVEKELASRYMFMVVATSSGDK
                     DPLKESSCIRLFREGRVDGVLIMSPVNDQSCIMELKKRNIPFVLLDQHQTNLQAPSVT
                     VNNFYGGYQATMCLIRGGAKSIGHITGSELFESSHERLRGYLKALDDSGIKQDERLVV
                     KGNFSFTSGYDAVKNWVMQGCLPDAVFAGDDNTAFGVIDAARELGIAIPERLAVIGYD
                     DHPFTSLFHPGISTVKQPAEKMAKCGVELLLKIIEGKVKRLTKISLKPTLILRGTTKP
                     PA"
     gene            complement(2696..4141)
                     /locus_tag="PLG72_05190"
     CDS             complement(2696..4141)
                     /locus_tag="PLG72_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ80226.1"
                     /translation="MENGKSKIKTGKSLLYSSGNFAASLTGSVFSTYVTFYYVDVLKM
                     PAALISLAMSIYGIWNAVNDPLLGQISDRTRSRWGRRIPYLLFGSFPFALFFILVWTP
                     PLQWLGGNTTAMFVYFISVVFLFDFLYTLVIINWTALFPEMYKTQEERTKVSTYRQVF
                     GIIANILGVALPPVLYTAFGWPFMGILFGILTLVFLYMSLAGSKEDPQAAANEGLPLL
                     KSLKATFSNRTFLIYVLAAMFVQFTFVMLQAALPFYAKYVLNIEGFQVSLMLGVIFIM
                     AMLWIPFWQKRANRIGSKKAIIISSALYAVSLLPLWFVKNYIGGIISSALIGIGLAGL
                     MLLLDVMLSDIVDEDELKTGARREGMYFGINGLMVRLAISFQSAIMGAVLSTSGYNAE
                     LPVNSQPESAIIGIKMLLVVIPVISVAIAILFYLKYPLYGKRLKEVKEQIRRLHEAPA
                     GAAPCESGPETVHAAELKLRQEDAENAGKHL"
     gene            4324..5097
                     /locus_tag="PLG72_05195"
     CDS             4324..5097
                     /locus_tag="PLG72_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ80227.1"
                     /translation="MMELKDFIPIPKLEECKSILCIQPHPDDNEIGAGATIAGLAAKG
                     CRVVYLTVTNGNMGSMDPEESPWETAEKRRKETLDAAGLLGVAECIFLDHDDGSFMDA
                     KKLCRDITAVIRKVRPDMVMTVDPCLPYEFHPDHLSVGMAAAQACIFSPFPHFDTKGC
                     AGLDGGGEAVPFQVKGIAFYSTAYPNTFINADETWELKMKAIEAHKSQFDPDTLETLK
                     SYFDFRARSYAAGLGFERAEAFKVLTAAHLHMNVDTVRL"
     gene            5176..6495
                     /locus_tag="PLG72_05200"
     CDS             5176..6495
                     /locus_tag="PLG72_05200"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ80228.1"
                     /translation="MNGIKLVVIGGGSSYTPELAEGIIARHRSFPVKELVLVDIEEGR
                     HKVESVFGLVGRMIAAAGLGIRLSWTLDRRSALDGADFVVSQIRVGGLEARSLDERLP
                     LKYGMIGQETTGPGGFANALRTIPAVMDICGDMEELCPNAWLINFTNPSGIITEAVLT
                     RTKIKCIGLCNIPINMERAVKNFPGLENADVHCGFAGLNHLSFMDSIMADGKEMIREL
                     TASAPNVFNAENIPGIGFPAEFISMLGMIPSPYLKYYYMESMMLEEERAKLRATGKTR
                     ALEVMEIEKGLFEKYRNPVLNKKPEELSKRGGALYSEAAVSLMDSIWNDRGDVQVVNT
                     LNGGCLPDLPENSVIEVNCVIGRRGALPVSHGPLAASIRGLIQHAKTYEQLTIEAACE
                     GSREKAFLALAANPLVHDIKNAKLLLDDLLEANRKYLTAFFGKGDAK"
     gene            6492..7424
                     /locus_tag="PLG72_05205"
     CDS             6492..7424
                     /locus_tag="PLG72_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ80229.1"
                     /translation="MIYLAGVDGGGTKTCCVIGDTDGNILARGISSGSNHQTVGPEAA
                     KEAIRDAMAQATDALGIATEDISYTVLGLAGADLEPDFAILDVICRDVIKTGRFRIMN
                     DTWIGLRAGVSENWGIVTVCGTGAACSGRNRYGAEATLRNLTYETGNYGGGIDILRMA
                     LHFAFRSEEGTGKKTRLEAELPAIFGAGSMADLVGPVVSMELDPEKAYHVPITVGRLA
                     AEGDAVCQDILLTTGRVLGEIASGIVKKLGMEQEQFKVALVGSVFKSVSPLLVDEYTT
                     TIHRTAPFAKVGISQTEPVMGAYYLALDAWRKHT"
     gene            complement(7825..9402)
                     /locus_tag="PLG72_05210"
     CDS             complement(7825..9402)
                     /locus_tag="PLG72_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80230.1"
                     /translation="MVHNPKARSRRWYARTVAMLLAATSMIFTLAGCSAGNGDEDADM
                     FPDGYEPPEIEKLEHTDLKFLFPGSEPASWKNVKAEIEERISDTLNASLEFRWVDFSQ
                     YFEILKVLDTSGEIYDALVVSKPEPMFPDFTVLAREGKLKDITDIFPSSAPLLFSKYT
                     GQELEYATVDGRLYAVPSLYPKARCSYLMVDDALFRKYNMPDITDYDKYEMFLKTIKE
                     NEPDLTPGAIANSISTLQLFARASGYVIADEAKRLVYKWDDPGMKLIPWEKTPEFHEA
                     VNRVISWYMNGYLACEPDYTRTASFFMEDMLAPPTDEPTKITILLTGTQEIKESNPFR
                     LFHLYPEKPVQRENPMGSFYSNGSFIFPAASDNTERVLQFLEWVQQSKENYYLLMYGI
                     EGKDYILTDGIPVFPENMDVFNSSYMYWGGWWAFVNLEYDPKAPVYNESGTETTLREF
                     LDRFSEYPPHGAFYPDYGPLQQAADDRSRIFNEFEINLLRGRILDPAKVDVFIRQLDE
                     LGSDELAEEAQEQMTKG"
     gene            9787..>10706
                     /locus_tag="PLG72_05215"
     CDS             9787..>10706
                     /locus_tag="PLG72_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ80231.1"
                     /translation="MVLLYVSMALGAFFILFGCFQLIFFRHRMNRTYRIIPSDERDED
                     TGVSVIHPVKGPVPELEKNLESWFRQNYTGPVQHIFSFHDPDDPAVPVVQKVMANHPE
                     ADCQITVDPAIPGLSGKSSGMACGMRLAKYDIVLFCDGDARVRQDFLVRMVRPLKDEK
                     VGMTTCAHVNTGGTDFWTRFFTFARNNVTDFIWALLGRFGLIFGTAGAAFAMRKRLLA
                     ELGGPEAFGGSLPEEAHLGSRLHRMGYRLVLGSFAECHADIIDKEEAVGYAKMTAISV
                     KARMAELSVFFLMLSWYWIIFILGFAFADPL"
BASE COUNT         2633 a         2551 c         2746 g         2776 t
ORIGIN      
        1 tgtgcattat gtttgaaagg aatctccttt tattaagcct cgagtaatcc ggtttccccc
       61 ggttcaggac aacgatctcg gaatcgatta tgcacttgtg cctgaactgt ttatatatat
      121 tgcggaattc cgggaaaagg gccgtgatat ctttattggc cctgttgcgg agaaccaccc
      181 cattgtcgta gtaaccgata cagcgcatcc cgtcaaactt tatctcgtaa atgtaatcgt
      241 tggaattaaa cggttcggac gacaccggcg ccatgggcac gatatttttt tcgtcaaaca
      301 tgtcgcggct cataatattt tcctctttcc gcctggtttt gactttgcgg aagtatcggc
      361 gcctgcgttt acgtccgcgg gttccccggc gccgttttct ccgacggccg gattcctgtc
      421 ttcagtggcc gttttcctta ttctattgct tgtgctcttt cccgtacttt tccctctgct
      481 tttctttttg ctgtccgtcc tgccgtgttt tgcggcttcc acgcttgctt tgagcgcttc
      541 catcaggtta attatgttcg actgcgcttc aggcggactt acaacttcct ttccctcgat
      601 cttggactga ataagctgct taagcgcatc ctggtactca ttcctgtatt gtccgatatc
      661 aaattccccg gtcatgttat cgataaggct tatggccatt ttcagctcat tgtcgtggat
      721 atccacgtta taattaagtt ccgtgatttc ttcggttttt ttgatttcat ccgaataata
      781 cagcaggtac agaagcatcc cgcctttgtc atacggcttt atcatggcca catactcatt
      841 ggacgtcagc gagattttcg caatgccaac cttgccggtt tcacgcatag cccttctgaa
      901 aagctcgaac gccttgcttg cgctgaaatt tccgggcgaa ataaaataag ccttatccag
      961 gtatatcgga tcgacttcgt cttcatccac aaaatgtgaa attgaaatta ccttttttga
     1021 cttaatgggc agtttttcga aatcctcgtc cgctataacg acatacttgt ccttctcgta
     1081 ctcgtatcct ttgacgatat catcctgaga aacttcctca ttgcaggcgt cgcaaaattt
     1141 cctgtaacgg atgcggctgt tgcatttcct gtgcagatag ttaagcttta ctccgactga
     1201 acccgaagtc gcagtatata cgttgacagg gaaatacacc atgccaaaac tgatggagcc
     1261 tttccaactc gtcttcattt ctgtcacctc tatttattcc gattgcataa acatccaacg
     1321 taaatttcag ctatatgctt gtcactgcgg atgtcagttt atatgcgttt atgtcccctg
     1381 cgtttttatt ttgtctgaaa aaaggtacaa atatttcgat ttcgctgttt gccatcaaaa
     1441 ttacaaattt gatccttcgg gctcttttta ttggaaatta tttggcactc aaatttgccg
     1501 atatactatt atggtgatgc ctattgagag tatctccggt agcaaagata gcaaaagcta
     1561 aactgttacg gagatacaaa agatttataa tttataagaa gcaaaagaaa aagaacgtga
     1621 acagcgagtt tacagagata tacaaaagga taaaggaagc ttaccaatag gtgggcgaca
     1681 taaacgatta tcagctcatt cttcttaggc cggcggtttt gtggtacctc tcagaatcaa
     1741 cgtcggttta agtgatattt ttgtcagcct tttgactttg ccttcaatga ttttcagaag
     1801 gagttccaca ccgcattttg ccattttctc tgcaggctgc tttacggtac tgatgcccgg
     1861 atggaacagg gaggtgaacg gatggtcatc atagccgata acagccagcc tctcgggtat
     1921 tgcaatgcca agctcccttg ccgcgtctat gacgccgaat gcggtattgt cgtcgcccgc
     1981 aaaaaccgca tccggcaggc atccctgcat gacccagttt tttaccgcgt cataaccgct
     2041 ggtgaatgaa aaattccctt tcacgaccag cctttcatcc tgttttatac cgctgtcatc
     2101 gagggctttc agataccccc tcaaacgttc atggctgctc tcaaacagct ctgatccggt
     2161 aatatggcct atgcttttcg cgccgcccct gatcaggcac atggttgcct ggtagccgcc
     2221 gtaaaaattg ttcacggtca cggatggagc ctgcaggttc gtttgatgct gatccagcag
     2281 gacaaagggg atatttcttt ttttcagttc catgatgcag gactggtcgt tcacaggcga
     2341 catgataagg acgccgtcaa cacggccctc cctgaaaagc cggatgcagc tggactcctt
     2401 cagagggtcc ttgtcgccgg agcttgttgc aaccaccatg aacatatacc tggaggcaag
     2461 ctctttctcc accgactgca tgacctgcac catgaaagca tcgtcaatgc acggtactat
     2521 aatgcctatc attccggttt tgcccctggc aagggtccgt gccgccgcat tgggcttgta
     2581 gtccagttcc tcgatggctg cgagcacctt tttcctgttg tattccctta cgccggggtg
     2641 gttgttgagt accttcgaga ccgttactac ggacaccccg gcctttttcg ctatatcata
     2701 gatgtttacc ggcattttcc gcatcctcct gccgtagttt caactctgca gcatgtactg
     2761 tttccggtcc ggactcgcaa ggcgcggcac cggcgggcgc ttcatgtagc ctccggatct
     2821 gctccttgac ttcctttagc ctttttccgt acaacggata cttcagataa aaaagtatcg
     2881 ctattgctac agagatcacc ggtatgacga ccaacagcat tttaatacct attatagcac
     2941 tttcgggctg ggaattgaca ggaagttccg cgttataccc cgaagtgctc aggaccgcac
     3001 ccatgatagc ggactggaaa gatatcgcaa gccttaccat cagcccgttt atgccgaaat
     3061 acatgccctc cctgcgggct ccggtcttca gttcgtcttc atcgacgata tccgaaagca
     3121 tgacatccag gagcagcatc aggcctgcaa gaccgatgcc gatcagtgcg gaggaaataa
     3181 tacctccgat atagtttttg acaaaccata aaggcagcag tgataccgcg tacagcgcag
     3241 aagatatgat gatcgctttt ttggagccaa tgcggtttgc acgcttttgc cagaaaggta
     3301 tccacagcat tgccattatg aatatgactc caagcatgag tgatacctgg aaaccttcga
     3361 tgttaagaac gtacttggca tagaaaggca atgcagcctg gagcataacg aatgtgaact
     3421 gtacgaacat ggccgcaagc acgtagataa ggaaagtcct gttggaaaac gttgctttca
     3481 gggatttcag cagcggcaat ccttcatttg ctgctgcctg gggatcttct tttgatccgg
     3541 ctaaactcat gtataaaaac accagcgtca ggatgccgaa caggataccc atgaaaggcc
     3601 agccaaatgc ggtgtaaagc accggaggaa gtgcgacgcc gagtatgttg gcgataattc
     3661 cgaagacctg cctgtatgtg gataccttcg ttctttcttc ctgggttttg tacatttcgg
     3721 ggaaaagagc cgtccagttg ataatgacga gtgtgtacag gaaatcaaac aggaatacca
     3781 cggatatgaa gtagacaaac attgccgtcg tattcccgcc cagccactgc agcggagggg
     3841 tccataccag tatgaagaag agagcgaacg ggaagctgcc gaacagcaaa tatggtatcc
     3901 ttctgcccca ccggcttctt gtcctgtcgg agatctgccc tagcaaaggg tcgttgacgg
     3961 cattccagat cccgtaaatg ctcattgcca ggctgatcag ggcggcgggc atcttcagga
     4021 cgtcaacata atagaacgta acataagtgg agaatacaga gccggtcagg gatgcggcaa
     4081 aattcccgga actgtacagg agacttttgc cggttttaat tttgctttta ccgttttcca
     4141 tattggcacc tcctgtaaaa ataaaagcgc tttaatataa taatattaaa aaatttgatg
     4201 atttacaata taaaaaatta aagcgtttat agatttctct taatggatgt gctaatatga
     4261 aaatgtgacc atgtcgattg ggcaagaaaa aagcatgaat gtaaaaatgg agaaaggggt
     4321 gccatgatgg agttgaagga tttcattccc ataccgaaac tggaggaatg caaaagcata
     4381 ctttgcatac agcctcatcc cgatgataac gagatcggtg caggagcaac gatagcaggc
     4441 cttgccgcaa aaggctgcag ggtggtatat cttacagtca caaacggcaa catgggaagc
     4501 atggaccctg aagaatcccc gtgggaaaca gcggaaaaac ggcgtaagga aacgctggac
     4561 gcagcgggtc tgcttggtgt ggccgaatgc atttttttgg atcatgatga cggcagtttt
     4621 atggatgcca aaaagctttg cagggatatc acggcagtaa taaggaaagt caggcctgat
     4681 atggtcatga ctgtagaccc ttgccttccg tatgaatttc acccggatca cctcagtgtt
     4741 ggtatggctg cggcacaggc ctgcattttc agcccgtttc cccactttga cacaaaaggc
     4801 tgtgccggtc tggacggcgg cggtgaagcg gtgccgttcc aggtcaaggg tatcgctttc
     4861 tacagtacgg cttatcccaa tacgttcata aacgcggatg aaacatggga actgaaaatg
     4921 aaggccatag aagcgcacaa gagccaattt gacccggata cattggaaac cctcaaatcc
     4981 tatttcgact tcagggcccg gagctatgcc gcgggacttg gatttgaacg ggccgaggct
     5041 ttcaaggttt tgacggcggc gcacctgcat atgaacgtag acacggttcg cctgtaaaag
     5101 gatgcgggcc ctgtcagccc ggtatgtaag aaatgaatac cggaagttct gcggcagata
     5161 aggaggacgg acaatatgaa cggtatcaag ctggttgtca ttggaggagg aagcagctat
     5221 acgcctgaac tggcagaagg catcatagcg cggcacaggt ctttcccggt gaaagaactg
     5281 gtacttgttg atatcgaaga aggcagacac aaggtggaaa gtgtcttcgg cctggtcgga
     5341 aggatgatcg cagcggccgg actgggcatc aggctcagct ggacccttga cagaaggagc
     5401 gcgctggatg gcgccgattt tgtcgtgtca cagatccgcg tgggcggcct tgaagcgcgc
     5461 agcctggacg agagactgcc cctgaaatac ggcatgatcg gacaggagac gacaggcccg
     5521 ggaggttttg ccaatgcgct ccggaccatt cctgccgtaa tggatatatg cggcgatatg
     5581 gaagaactgt gtcccaatgc gtggcttatc aacttcacga acccttcggg gattattacc
     5641 gaagctgttc tgacccgtac aaaaatcaaa tgcatcggtt tgtgcaatat cccgataaac
     5701 atggaacgcg cggtaaagaa cttcccggga cttgaaaatg cggacgtgca ctgcggattt
     5761 gccggactga accatttgag ctttatggat tcgatcatgg ccgacggcaa agagatgata
     5821 agggagctga ccgcttcggc gccgaatgtc tttaacgcgg agaacatccc gggcatcggg
     5881 ttcccggcgg aattcatatc catgcttggc atgataccgt cgccttactt gaaatattac
     5941 tatatggaaa gcatgatgct tgaggaggaa cgggccaaac tgcgtgcgac gggcaagacc
     6001 agggcgcttg aggtaatgga gatagaaaag ggcctgttcg aaaagtacag gaacccggta
     6061 ctgaataaga aaccggaaga actgtcgaaa cggggaggag cgctgtactc cgaagcggcg
     6121 gtgagcctga tggacagtat ctggaatgac aggggagatg tacaggtggt gaatacgctg
     6181 aacggcgggt gcctgccgga tctgcctgaa aattcggtta tcgaggtcaa ctgtgtcatc
     6241 ggccgccgcg gtgcgctgcc tgtcagccac gggccgcttg ctgcttcgat acgcggactt
     6301 atacagcatg caaaaacata tgagcagctg acgatagagg cggcatgcga aggaagcagg
     6361 gagaaggcct ttcttgcact ggctgccaac ccgctggtac atgatataaa gaacgcaaaa
     6421 ttgcttcttg atgatttgct ggaagccaac aggaaatacc tcactgcatt tttcggaaag
     6481 ggtgacgcaa agtgatctat ctggcgggag tcgatggcgg aggtacgaaa acatgctgcg
     6541 tcattggtga tacggacggt aatatacttg ccagggggat ctcatcaggg tcgaaccacc
     6601 agacggtagg ccctgaagcg gcaaaggagg caataaggga cgcaatggcc caggcaacgg
     6661 atgcgctggg tattgccaca gaggatattt cctatacggt gctggggctt gcaggagcgg
     6721 acctggagcc ggattttgcc atactggatg ttatttgcag ggatgttata aaaaccgggc
     6781 ggttcaggat catgaacgac acgtggatag ggcttcgtgc cggcgtctcg gaaaactggg
     6841 ggatagtgac agtatgcggg acaggggctg cctgttccgg caggaacagg tatggagcag
     6901 aagccacact gaggaatctt acctatgaaa ccggcaacta tggcggcggt atagacatct
     6961 tgcgcatggc tttgcatttc gcattccgtt cggaagaggg aaccggtaag aagaccaggc
     7021 tggaggcgga attgcctgcg atcttcggcg ccggcagcat ggcggacctg gtgggaccgg
     7081 tcgtctcaat ggaactcgat ccggaaaaag cgtatcatgt tccaataacc gtaggcaggc
     7141 ttgccgctga aggcgatgcg gtgtgccagg atattttgct tactacaggc agggtccttg
     7201 gcgagatcgc ttcagggatc gtaaaaaagc tgggaatgga acaggaacag ttcaaagtgg
     7261 cgcttgtcgg aagtgttttc aaatcagtca gcccgctgct ggtggatgaa tatacgacta
     7321 ccatccacag gacagcgcct tttgccaagg tcggcatctc acagaccgaa ccggtcatgg
     7381 gcgcctatta ccttgcactt gatgcctggc gaaagcatac ctgaggcgga aagcaagcct
     7441 gcaaccggtt ggacagagca acggatcaga agtccgttgg ccggagcaga tttatatacg
     7501 gcactcaaaa cttgctctca aatccggcaa agcccaacat ggcggtcaac ctgccttcat
     7561 ctgctcatat atcatatcag cgagttcatc gctgccgagc tgatccagtt catttatgaa
     7621 ggcgtcaact tcactggttt ccagtatctc tccctttacg agtttataat cgaactgatc
     7681 gaagagtgcg gctcttctat ctgctgactg ctgcagcgct ccgtaattcg ggtagaaagc
     7741 tccgtgagga gggtattgtg tgtgttatat tacccggaaa gcaaaccgcc gtatctgatg
     7801 cccgggatat cgaaattgaa gtggttatcc ctttgtcatt tgttcctgcg cttcttcggc
     7861 aagctcgtcg cttccgagtt cgtcaagctg ccttataaat acgtccactt tagcgggatc
     7921 cagtattctg ccacgcagga gattgatctc aaactcgttg aagatcctgg acctgtcatc
     7981 cgctgcctgc tgcagcggcc cataatccgg atagaaagct ccgtgaggcg gatattctga
     8041 aaacctgtcg agaaactccc ggagtgtcgt ttcggttccc gattcgttat atacgggagc
     8101 tttcggatca tactccaggt ttacgaaggc ccaccagccg ccccaataca tgtatgaact
     8161 gttgaagaca tccatatttt cggggaatac aggaataccg tctgtcaata tgtaatcctt
     8221 gccttcgatg ccgtacataa ggagataata gttttctttg ctctgctgca cccattcaag
     8281 gaattgcagt accctttcag tattatctga agcggcggga aatatgaagg agccgtttga
     8341 gtagaagctt cccattggat tttccctctg gaccggcttt tcaggataaa ggtgaaacag
     8401 cctgaaagga ttggattctt ttatttcctg cgttcctgtt aaaaggatgg taatctttgt
     8461 cggctcatcc gtcggcggag ccagcatatc ttccataaaa aatgaggcgg tcctcgtata
     8521 gtcgggttcg catgcaaggt atccgttcat gtaccagctg atcacgcggt ttaccgcttc
     8581 atggaattca ggtgtctttt cccacgggat cagcttcatc ccgggatcgt cccatttgta
     8641 cacaagcctt tttgcttcgt cggcgatcac atagcccgac gcccttgcga aaagctgcag
     8701 agtgctgatg gaatttgcta tggctcccgg cgtcaggtca ggttcattct cttttatggt
     8761 tttcaggaac atctcgtatt tatcataatc ggtaatgtcc ggcatattat actttctgaa
     8821 aagcgcgtcg tcgaccatga ggtatgaaca gcgtgccttc ggatacagcg acgggactgc
     8881 ataaagtctg ccgtccactg tcgcgtattc cagttcctgg cctgtgtatt ttgaaaacag
     8941 gaggggcgcg cttgagggaa atatatcagt aatgtctttc agtttcccct cgcgtgcaag
     9001 gaccgtaaaa tccggaaaca tcggctccgg ctttgatacg accaaagcat catatatttc
     9061 gcctgacgtg tccagtactt ttaatatttc aaaatactgt gaaaaatcga cccatcggaa
     9121 ttcaagagag gcattcagcg tatcgcttat gcgttcctcg atctcggcct tcacattttt
     9181 ccagcttgcc ggctctgatc cgggaaagag gaatttgaga tccgtgtgtt ccagtttctc
     9241 gatttcgggc ggttcatatc cgtcagggaa catatccgca tcttcgtctc cgttccctgc
     9301 gctgcagcct gcgagcgtga agatcatgct cgttgctgcc agcagcatgg caaccgtcct
     9361 ggcataccat cgtctgcttc ttgcttttgg attgtgtacc atcttatctg acacttcctt
     9421 ataaatatca gcttataaag ttatgcctgg ctatccggat ataattttat gttcagtccg
     9481 atatcaggca gagatgtcga ttttatgcta ttgggtcgct tttcttcgaa tttgaccggg
     9541 cttttttcac accgtatata actgttgcca tcattttaca catcccgatt acaagaaaaa
     9601 aggcataaaa cttgaataat ctgtaaattt tttatgaaca cgactacacg gccgaagcat
     9661 atcatggcgg atgcccggaa gaaccgctgg cggttgtaat aataacagat aggttatata
     9721 atagtacttg ctgttgaaaa atgcgcatag tatatttgct tacctgtgtg cgggggggat
     9781 ggataaatgg tgcttctgta cgtaagtatg gcgttgggag cttttttcat actgttcggg
     9841 tgtttccagc ttattttctt cagacaccgc atgaacagga cataccgcat cataccgtcg
     9901 gatgaacggg atgaggatac gggcgtgtcc gtcatccatc cagtgaaagg tccggttcct
     9961 gaactggaaa agaacctgga atcatggttc aggcagaatt acacgggccc cgtgcagcat
    10021 atcttcagtt tccacgatcc tgacgatcct gccgtaccgg tggttcaaaa ggttatggcg
    10081 aaccatccgg aagcagactg ccagattact gtggacccgg ccatcccggg attgagcggc
    10141 aaaagctccg gcatggcctg cgggatgagg ctggcgaagt acgatatcgt acttttctgt
    10201 gacggcgatg ccagggtcag gcaggatttc ctggtcagaa tggtaaggcc cctgaaagat
    10261 gagaaggttg ggatgacgac ctgcgcgcat gtcaatacgg gcgggaccga tttctggaca
    10321 cgttttttca catttgcccg aaacaacgta acggatttca tctgggcatt actgggcagg
    10381 ttcgggttaa ttttcgggac cgccggagca gcttttgcga tgaggaaaag gctcctggct
    10441 gaattaggcg ggcctgaagc ttttggcggc tccctgccgg aagaagctca cctggggagc
    10501 agattgcaca ggatgggata caggctggtg ctggggtctt tcgcagagtg ccatgcagat
    10561 attatagaca aagaggaggc cgtcggatat gcaaaaatga ccgccatcag cgtgaaggcc
    10621 cgtatggcgg agctttccgt gttcttcctg atgctgtcct ggtactggat catattcatc
    10681 ctgggttttg ctttcgctga tccgct
//
LOCUS       DAOSCC010000069        10684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4088_length_10684_cov_5.755574, whole genome shotgun sequence.
ACCESSION   DAOSCC010000069 DAOSCC010000000
VERSION     DAOSCC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_10684_cov_5.755574"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLG72_05220"
     CDS             <1..1252
                     /locus_tag="PLG72_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOJ80232.1"
                     /translation="ATDHNQLMMELMKTVAARHNLACLLHEKPFAGINGSGKHVNWSI
                     STDTGINLLEPGDTPFENAQFLLFLVAVIKAVDDYQDLLRVSVASAGNDHRLGANEAP
                     PAIVSIFLGEELTEILNALENDTLYTCKGQTLMEIGATVLPHFPKDTTDRNRTSPFAF
                     TGNKFEFRMLGSAFSIAGPIIVLNSIVAEVLSQFADELENSTDFKSDLSKLIKRTIKN
                     HKRIIFNGNGYDASWVEEAERRGLSNLKTTPEALPAFIHPKNVEMFVRHGVFTEHEIH
                     SRYEILLENYSKTIRIEALTMTDMVNKYVIPAVISYQNELAELVLNKKAVNAGIDTSL
                     EENRLKRISELSVELEKRLGNLAEQTQAVSKITDTLESARAYREKVFTAMNDLREVID
                     ELELITSSKHWTLPTYTDILYSVI"
     gene            complement(1425..1928)
                     /locus_tag="PLG72_05225"
     CDS             complement(1425..1928)
                     /locus_tag="PLG72_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80233.1"
                     /translation="MNTIVERTVSLGKPIMAPCMVPVEHKSTPNCINDPFMVNGECWK
                     VTAMSFGSPHGAVFVDDVDATDVRNIGPALGTHSLFPEGASIVFIQILDRENVKARLW
                     HREKGETDFTAEAACVAGVTAAMLHRIPASEVNIHMGQNIFRVEWNRVTDEVCLTGPA
                     DVLGTAG"
     gene            complement(2002..3333)
                     /gene="gdhA"
                     /locus_tag="PLG72_05230"
     CDS             complement(2002..3333)
                     /gene="gdhA"
                     /locus_tag="PLG72_05230"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOJ80234.1"
                     /translation="MSYTMRVLERVIKQNPNEPEFHQAVTEVLTSLQPFVDSHPEYEE
                     AGILERLCEPERVIIFRVPWVDDSGKVRVNKGYRVQFNSAIGPYKGGLRFHPSVSLSI
                     IKFLGFEQVLKNSLTGLPMGGGKGGSDFDPKGKSEREIMAFCQSFMTELYRHIGENTD
                     VPAGDIGVGEREIGYLFGQYKRIIDSFSGVLTGKGLSYGGSLGRPEATGYGLVYLVEE
                     MLNRHGSSLEGKTVAISGAGNVAIHAVEKVCQLGGRTVTMSDSDGFVYDPDGIDLQVV
                     KEIKEIRRGRISEYVNYRKSAVYTPGRGVWNVKCDVALPCATQNELNEEDAEELIKNG
                     VIAVAEGANMPTTLGATELFIKNRVLFVPGKAANAGGVAVSGLEMSQNSARIAWSFEE
                     VDRKLREIMINIYNNISSVAKEVGKEDNFVVGANIAGFKKVADAMLRQGVV"
     gene            complement(3536..3688)
                     /locus_tag="PLG72_05235"
     CDS             complement(3536..3688)
                     /locus_tag="PLG72_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80235.1"
                     /translation="MWSTGNESGHGPNHLAMISGPGRRTTRVLSTVKTCRGKTVPVRY
                     QLNEKS"
     gene            complement(3983..4561)
                     /locus_tag="PLG72_05240"
     CDS             complement(3983..4561)
                     /locus_tag="PLG72_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOJ80236.1"
                     /translation="MDSALIVSYSKKVADSLAEILAQTSVTDVATLSNGGEARRMMIE
                     KDYDLYIINTPLPDEFGDDLALDIASKGISVVILIVKAELYDEISEKVENYGVITMAK
                     PISRTIFWNALKLSMAAHRKIKAIHNENTKLMQKIEDIRIVDRAKCVLISKFSLTEPE
                     AHRYIEKKAMDTRRTRRKVAEEILMLYENNML"
     gene            complement(4608..5927)
                     /locus_tag="PLG72_05245"
     CDS             complement(4608..5927)
                     /locus_tag="PLG72_05245"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOJ80237.1"
                     /translation="MITTVDQVLEFIHENDVKFIRLSFCDLFGNLKNISIIPDELPRA
                     FEHGIPFDAHAVSGFTDITKSDLLLVPDPATLSVLPWRPQQGRVVRFYCNIKNPDGSV
                     FWGDTREILRSVVDRTRQKGYVCMIGAECEFYLFVTDENGEPTSVPFDNGGYLDVYPL
                     DKGENIRREICICLEEMGIRPETSHHERGPGQNEVDFKFSDAVNSADNFITFKQVVKS
                     IAARNGLFASFSPKPLPDKSGSGLHVNLSLMQNGQNIFRIGEKENSKVAESFVAGVLQ
                     KISEITAFLNPIPNSYERLGACEAPKYISWSYGNRSQLVRIPADSDERTRMELRSPDP
                     ATNPYLAFALIISAGFYGIENGLALPESINVDLYNADENVIGTLPRLPENLGHALKLA
                     EKSDFLKNTLSEQVIDSFVRLKNLELQTYKRTADKRVYFLENYFSVI"
     gene            complement(6964..7764)
                     /locus_tag="PLG72_05250"
     CDS             complement(6964..7764)
                     /locus_tag="PLG72_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ80238.1"
                     /translation="MFEEFYGLSATPFSRDIPTSELYPSIALEETLGRLEYAAQRQLF
                     AILTGDCGIGKTTAIRKFKDVLSPSEYLVMYLSDSKLTPRHFYKGLLEQLGCESKFYR
                     GDAKRQLHREIELMRGIQHLQPVVIVDEAHLLDREMLEEVRFLLNFKLDSQSPMALIL
                     VGQSELWEKLQLQAYAAIRQRIDIQCRLQYFDRAQVEEYMKRHMSYAGASRDIFTEAA
                     IDEIFKFSNGAARLINKVCSHCLFYGAQNGRRIIDDHMVKLVIQGELA"
     gene            complement(7757..9112)
                     /locus_tag="PLG72_05255"
     CDS             complement(7757..9112)
                     /locus_tag="PLG72_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOJ80239.1"
                     /translation="MRDEKKAEGIAVWRVQMLSPLLQEGLDPAKARQIRARICEETGL
                     SDRSIRRYIARYREKGFEGLKPKGKEKTNREDPIPPHILEQAILLRREVPGRSVSQII
                     QILEWEGRVAQGQIKRSTLQEKLAQKGYSARHMKMYAQSGVAARRYQHRYRNKLWHSD
                     IKYGPYLPIGKDGMKKQVYLVLFVDDCSRYVFHGEFYPVLDQVIVEDCFRKAIQKYGV
                     PETVYFDNGKQYRTKWMTRACSKMGIRLLYAKPYSPESTGKPERLNRVVDSFLSEAAL
                     EKPQTLDKLNELFWVWLEECYQNKPHSALEGKASPEAAYRSDKKPLRFLEPEVIANAF
                     LHCEERKVDKAGCISFEGKKYEVGLSFIGCTVDVIYDPSDTSELTIEYEGHAPFKAKQ
                     LVIGEHAGRRPKLPEHMNTVPAESSRLLAAAAKQNQQRQEHQATAISYRSLSKGGSRN
                     V"
     gene            complement(9164..9706)
                     /locus_tag="PLG72_05260"
     CDS             complement(9164..9706)
                     /locus_tag="PLG72_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HOJ80240.1"
                     /translation="MINVKEYELIPHKKAQDKYHVKSKEKSICPVCGSLELKVVGTRT
                     RKVLKSCGKKIILVIRRLRCRSCRRIHHELPDSIVPYKRYSSESIEAILDGMEDTVSC
                     EESTIYHIRKWFSDFSGYLAGSLDAIAVRLGLKTVVGHKSPMSRIKTYVGDESGWLAR
                     VVRPLVNSNMWVHTRSAFLS"
     gene            complement(9710..10465)
                     /locus_tag="PLG72_05265"
     CDS             complement(9710..10465)
                     /locus_tag="PLG72_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOJ80241.1"
                     /translation="MERYKIADEKIVSGYDYLERGNSQKACDMWLEAWDDIKSIMSSI
                     GIDNIDKLQQKYQWSEFLSNYVQDLEAELHNTGLENKEYFRKRIKYCEEMLAVCGDED
                     DLIIENTRRAIADSHFELGNEEECDRLYSMWLGADPNWGWGYIGWSDCYHFGKNKTEA
                     HLRKAEAIITKALEQKDLRDRLDVIERAVEIYQALGNNEKVAELKKEIPSLKRSTFVK
                     HTTAGPIKTEKTGRNDPCPCGSGKKYKKCCGKS"
BASE COUNT         2578 a         2834 c         2231 g         3041 t
ORIGIN      
        1 cgcgacggac cacaaccagt tgatgatgga actgatgaaa accgtcgccg caaggcacaa
       61 ccttgcatgc ctcctgcatg aaaagccttt cgcagggata aacggcagcg gaaagcacgt
      121 caactggtcc atatcgaccg atacgggcat aaacctgctg gaacccgggg acacgccttt
      181 tgaaaacgca cagttcctgc tgttcctcgt cgcagtgatc aaagcggtgg acgactatca
      241 ggacctgctg cgcgtatcag tcgcaagcgc cggcaacgac caccgtctcg gcgccaatga
      301 agctcctcct gccatcgttt cgatcttcct tggcgaagag cttaccgaga tcctcaatgc
      361 gctggagaac gatacgctat atacatgcaa agggcagacc cttatggaga tcggcgccac
      421 ggtgctgccg cacttcccga aagacacgac ggaccgcaac cgtacttcgc ccttcgcgtt
      481 cacaggcaac aagttcgagt tccgtatgct tggttccgca ttctccatcg caggtccaat
      541 tatcgtactc aactcgatcg tagccgaagt actgtcacag tttgccgacg agcttgaaaa
      601 cagcactgat ttcaagtctg acctgagcaa actgatcaaa aggaccatca agaaccataa
      661 gcgcatcatt ttcaacggca acggctatga tgcctcatgg gtggaggaag ccgaacgcag
      721 gggcctgtcg aacctgaaga ccaccccgga agctttgccg gcgttcatcc atcccaaaaa
      781 tgtggaaatg ttcgtccgac atggagtctt cacggagcat gagatccatt cacgatacga
      841 gatattgctt gaaaattact cgaaaaccat caggatcgaa gctctcacta tgaccgatat
      901 ggtgaacaag tatgtgatcc ctgccgtgat cagctatcag aatgaacttg ccgaactggt
      961 actcaacaaa aaggctgtga atgccgggat cgatacttcc ctcgaggaga accgcctcaa
     1021 gaggatatcc gaactgtcgg tcgaactgga aaagagactg ggcaatctgg ccgagcagac
     1081 ccaggcggtc agtaagatca cagacactct tgaaagcgcc cgggcatacc gtgaaaaagt
     1141 gtttacggca atgaacgacc tgcgggaggt catcgacgag cttgaactga tcacaagcag
     1201 caagcactgg acgctcccga cctacacgga catcctttac agtgtgatat agcgatccac
     1261 ataagcgatc cacacttccg caacaaaggg aaaaacccac agccgagcga taaacgctcg
     1321 atgctgtggg ttttcccttg agcaacacat ccctttataa caaactcaca ttttatgatc
     1381 cgtatgggcg tttattgcct atcggatcat acaactgtga gttgttaccc tgccgttccc
     1441 agcacatcgg ccggccctgt caggcagacc tcgtccgtca ccctgttcca ttccacccgg
     1501 aaaatgttct gccccatgtg gatattcact tcacttgccg gtatcctgtg gagcatcgct
     1561 gctgttaccc ctgcgacaca ggcggcttca gcggtgaaat ccgtttctcc cttctcgcga
     1621 tgccaaagcc gtgccttgac attctccctg tccagtatct ggatgaaaac tatgctcgca
     1681 ccttccggga acaggctgtg cgtaccaaga gccgggccta tgttccggac gtccgtggca
     1741 tcgacatcat ccacgaacac tgcaccatga ggcgaaccaa aggacatagc cgtgaccttc
     1801 cagcactcgc cgtttaccat aaacgggtca ttgatgcaat tcggtgtcga tttgtgctcc
     1861 accggtacca tgcacggagc catgatcggc ttcccaagtg aaacagtcct ttcgacaatt
     1921 gtgttcatcc tatcgcctcc tttaacgata ctgctgactt ttctttttcc ttgagcttat
     1981 actttgcaat aatggtttgg tttaaactac accttgccta agcattgcgt cagcaacttt
     2041 cttgaaccct gcaatattcg ctcctactac gaaattgtct tccttcccta cttctttcgc
     2101 tacgctggaa atgttgttgt atatgtttat cattatttcc ctgagttttc tgtccacttc
     2161 ctcgaaactc catgcgatcc tggctgaatt ctggctcatc tcaagtccgg atacagcaac
     2221 gccgcctgca tttgcagctt ttccgggtac gaacagcacc ctgttcttta tgaacagttc
     2281 agtagctccc agtgtggtcg gcatgttggc cccctccgca acggcaatga caccgttctt
     2341 tatcagttct tccgcatctt cctcgttaag ttcgttctga gtcgcacacg gaagcgccac
     2401 atcgcacttg acgttccata cgcctcttcc cggggtatat acagcacttt tgcggtaatt
     2461 tacatattcg gagatcctac cacgtcttat ttcctttatc tccttgacaa cctgcagatc
     2521 gataccatcc ggatcataca cgaaaccgtc agaatctgac atggtgactg tcctgccgcc
     2581 cagctgacag accttttcga ccgcgtgtat cgccacattt cccgcgcccg aaattgcaac
     2641 agttttccct tccagcgagc tgccatgccg gttgagcatt tcctcgacca ggtataccag
     2701 cccgtaaccg gtagcttcag gcctgccgag cgatcctccg tatgacagtc ccttgcccgt
     2761 gagcacaccg ctgaacgaat cgattatcct cttgtactgg ccgaaaaggt atccgatctc
     2821 acgctcgccg actcctatat ctccggcagg tacgtcagtg ttttctccta tatggcggta
     2881 caattccgtc atgaaactct ggcagaaagc cattatctca cgttccgact tgcccttcgg
     2941 gtcgaaatcc gatccgcctt tccctccgcc catgggcagt ccggtaagcg agtttttgag
     3001 gacctgctca aacccaagga attttatgat actcaggctg acgctcggat ggaagcgaag
     3061 gccgcccttg tacggaccga ttgcactgtt gaactggacc ctgtagccct tgttaaccct
     3121 cacttttccc gaatcatcga cccacgggac cctgaagatt attactctct ccggctcgca
     3181 aagcctctcg agtatgcctg cctcttcata ctcgggatgc gaatcgacaa aaggctgcag
     3241 ggaggtcaat acctcagtca ccgcctggtg gaattccggt tcattcggat tctgttttat
     3301 cactctctca agtaccctca ttgtgtaaga catcgttgta accctcctgt cgaataaaaa
     3361 aaagacgcct accaacacac tggtacgcac cagtgcgttg ataaacgtct ttgtttatcg
     3421 ctacaagtat aacagcaatt ttaccgtatt gtcaatacag cactgtttta tgttgagctt
     3481 atcctgcaga aaccgggttc cccaaaccgg cccggctgta aggtgaaacg gaatttcaac
     3541 ttttttcgtt caactggtat cgtacaggca ctgttttccc tcgacacgtc ttcacagtgg
     3601 ataagactcg tgttgtcctt ctgcctggtc cacttatcat tgcaagatga ttggggccgt
     3661 gtccgctttc gttccctgtc gaccacatta ttacacacag attgttttta atatttcggc
     3721 catcctctgc atgcgctcca cgtgttcttt ttacaattcg ggcattgtac acggccattc
     3781 gccggtctcc gtatcatacc ggtacggcac gttgggcagc cggcgaaaaa acccgtaagt
     3841 ttcgatatca gtctcgcata tcacgtaaaa acccaactta ctgacgtttt tattgcaagg
     3901 gagattcgat aaaataataa accccgcggc aaatttggat tctgcacttg ctgcagggtt
     3961 tggaacggac ttcagtatgc tctcagagca tgttgttttc atatagcatc agtatttcct
     4021 ccgcgacctt cctgcgcgtc ctgcgcgtat ccatcgcctt tttttcgatg tacctgtgtg
     4081 cttcgggctc agtcaatgag aacttggaaa tgagaacaca cttggctcgg tcgacgatcc
     4141 ggatatcttc gatcttctgc atcagtttcg tgttttcatt atgtatcgct tttattttcc
     4201 tgtgtgcagc catggacagt ttcagggcat tccagaaaat ggtcctgctt atgggtttcg
     4261 ccatcgtgat aacgccataa ttttcgacct tctctgagat ctcatcgtat agttcagctt
     4321 ttacaatcag gatcaccaca ctgatccctt ttgaagctat atccagcgcg aggtcatctc
     4381 caaactcgtc gggcagggga gtgtttatga tatacaggtc gtaatccttt tcgatcatca
     4441 tcctcctggc ttccccgccg ttggacaagg tggcgacatc cgtcactgat gtctgtgcca
     4501 gtatctctgc caaactgtct gcaacttttt tcgaatatga tacgattagt gcactgtcca
     4561 tcgcgtacca ccttttactt tacgaaaact gtcaatgggg tcgtctgtca aattacacta
     4621 aaatagttct ccaggaaata tacccttttg tcggcagtcc gcttatatgt ctgcaattca
     4681 agatttttca aacgtacaaa gctatcaatg acctgttcgc tcagcgtgtt tttcaggaaa
     4741 tcggactttt cggcaagttt cagagcgtgt ccgagatttt caggaagcct cggaagcgtg
     4801 ccgatcacgt tttcatcagc gttatagagg tcaacattta tgctttcagg cagtgcaagg
     4861 ccgttctcta tgccgtaaaa tcctgcggaa attatcaatg cgaaagcaag gtacggattc
     4921 gtcgcaggat cgggagatct tagttccatg cgcgtcctct cgtcgctgtc ggcagggatg
     4981 cgtacaagct gcgaacggtt gccgtaggac caggaaatat actttggcgc ctcgcaggct
     5041 ccaagacgct cgtatgaatt gggaatcgga tttaaaaacg ctgttatttc agatattttc
     5101 tgaagcacac ctgcgacaaa ggattcggcc acctttgagt tttccttctc acctattctg
     5161 aatatgttct gtccgttctg catgagtgac aggttcacat gcagtccgct cccgctttta
     5221 tcgggcagcg gtttcggcga aaacgacgcg aacaggccgt tccttgccgc gatacttttc
     5281 acgacctgtt tgaaggtaat gaagttgtct gcgctgttga cagcatcgga aaatttgaaa
     5341 tcgacctcat tctgtccggg tccccgttca tggtgggatg tttcaggcct gatccccatt
     5401 tcctccaggc agatgcagat ctcgcgcctg atgttttcgc ccttgtcgag aggatataca
     5461 tccaggtaac cgccgttgtc aaacggaacg gaggtgggtt ctccgttctc atcggttacg
     5521 aaaaggtaga actcgcattc tgcgccgatc atgcacacgt atcctttttg cctggtccga
     5581 tccacaacgg atctgaggat ctcccttgta tcaccccaga aaacgcttcc atcgggattc
     5641 ttgatgttgc agtaaaaacg gaccaccctg ccctgctgag ggcgccatgg caaaacggac
     5701 agtgtcgccg ggtccggaac cagcagcagg tcggacttcg ttatatccgt aaatccgctt
     5761 accgcgtggg catcgaacgg gatgccgtgt tcgaacgcac ggggcagttc atccggtatg
     5821 atcgaaatat ttttcaggtt cccgaacagg tcgcaaaatg aaagacgaat gaatttcaca
     5881 tcattttcat gtatgaattc caggacttga tcgactgtag ttatcatttc tctaccctcc
     5941 gatttttctt tataacaaca ttataccaac ggcagtaccc tttcgtaatt attagcacta
     6001 aactcaagtt attcccttta tatgcagtcc ttataactaa aaatggcgcc cattacataa
     6061 tgcacacagt taatatccgg ctgaaacccg gtttaactga aattgcatta tctaatgaac
     6121 gccattgttc attctttttt attatatggc gcttacagct tgtttgtcaa cgacattcag
     6181 ccgatttgtg acaaatggga ctgcgtcctc tctgaaagca cactaataca atgggttggg
     6241 aattttgcag gaccgtggaa ccttcacttg caaaaacagt ctgttttatt gatacgatga
     6301 tttcatcaat cagttcatga aaggatgatg tgctgtattc catctgcctg agcgggcaaa
     6361 atcatagtaa tctccaaaac aaggccagga aaattcctat cggtcaagac cacatcttta
     6421 ggttatcttt cattaccata atccttttaa attaattatc aaactgaaag cataaatcct
     6481 attatagggg aatttattat cattgccaaa ctatcctgtt actgccaacc tatagtgtcc
     6541 cccaatagac agtgtttggt gtcatgaggt tgacgatgat gccgtccaaa ataccggagc
     6601 tagggcgggt acctaatagg ttactccgcc actagctcca gactggttat tgaaactcca
     6661 agacatttgc taataccttt agtgcgcaag cgacagttct tccgtcaact gggttctgca
     6721 ccgcattgat taacttttca ctgttgtaaa agatatcatc cagctcactc agattacaac
     6781 cagcgcagta atccggaaga catgcaaata gccctgatgt gcacttccca attagtactc
     6841 gaatactcga ccggcttgta atgaatatct cgtaaaaact gccaaagctg gtgatttgac
     6901 tgattacacc ataccagtca tcccgttcat tatcatccca ctggtaagag cactggaacc
     6961 gcatcatgcc aattccccct gtattacaag ctttaccatg tggtcatcaa tgatccgtcg
     7021 gccattctgt gctccgtaga acaagcagtg agaacagacc ttgttaatga gtctcgcagc
     7081 tccgttcgaa aatttgaaaa tttcatcaat agcagcttct gtgaatatat ctctgctagc
     7141 ccccgcatag ctcatatgcc gtttcatata ctcttcaacc tgagcacggt caaagtattg
     7201 tagtctgcat tgaatatcaa ttctctgcct tatggcggca taagcctgaa gctggagttt
     7261 ttcccacagt tcactttgac ctacgaggat gagtgccata ggactttggg aatcaagctt
     7321 aaagttaagc aaaaaacgga cttcttccag catttcacgg tctaataggt gcgcctcatc
     7381 aactattaca accggctgta aatgctgaat tcctcgcata agttcaattt ccctgtgcag
     7441 ttggcgtttt gcatccccac gatagaattt agactcgcag cctaactgct ctaaaagccc
     7501 tttgtagaaa tgccgcggcg tgagcttgga gtccgacaga tacatgacca gatactccga
     7561 tggactcagc acatccttga atttgcgtat ggctgttgtc tttccgattc cacaatcgcc
     7621 ggtaagtata gcaaacaact gacgctgggc agcatactca agacgaccca gtgtctcctc
     7681 cagagctatg gatggataga gttcactcgt tggtatatct cttgagaatg gggtggcgga
     7741 aagtccatag aattcctcaa acattcctgc tgcccccttt gctgagtgat ctgtaggaga
     7801 tagctgtagc ctgatgctcc tgcctttgct gattttgctt tgctgctgca gctaggagtc
     7861 ttgatgactc tgcaggcacg gtgttcatat gctcaggaag cttgggacgc cggcctgcat
     7921 gttccccgat taccagctgt tttgctttaa aaggtgcatg cccttcatat tcaatagtca
     7981 attccgaggt gtctgaagga tcatatataa catccacagt acagccgata aaagaaagtc
     8041 ctacctcata cttcttacct tcaaagctta tgcacccggc tttatcaacc ttgcgctctt
     8101 cacaatgcag gaaagcattt gctattacct caggttctaa aaaccgtaaa ggtttcttgt
     8161 cactacggta tgcggcctca ggactggctt tcccttcaag ggctgaatgc ggcttgttct
     8221 ggtagcattc ctcaagccac acccagaaca gctcattcag cttgtctagc gtttgaggct
     8281 tctccagtgc tgcttcactg agaaaggaat ctacaacccg atttagtctt tccggttttc
     8341 cggtagattc cggggaatag ggctttgcat atagcagccg gatgcccatc tttgaacagg
     8401 cacgagtcat ccattttgtc cggtactgct ttccattatc aaagtataca gtctctggaa
     8461 ccccatactt ctgtatagcc ttgcggaagc aatcctccac tatgacctgg tctaataccg
     8521 gatagaactc tccatggaag acatatctgc tgcagtcgtc tacaaaaagt acaaggtata
     8581 cctgtttttt catgccatct ttaccgatag gaagatatgg gccatacttg atatccgaat
     8641 gccaaagctt gttccgatat ctatgctgat accttcgtgc agccacacca gattgggcat
     8701 acattttcat gtgtcgggca ctgtatccct tttgtgccag cttctcctgc agagtgctgc
     8761 gttttatctg cccttgtgct acccgccctt cccattcaag gatctgaata atctgtgata
     8821 cactccttcc aggaacttcg cgacgaagga gtatagcttg ttccaatata tgtggaggaa
     8881 tgggatcctc cctgtttgtt ttttccttcc ctttaggctt gagcccttca aatcccttct
     8941 ctcggtatct tgcaatgtat ctgcgtatac ttcgatctga cagacctgtt tcttcacaga
     9001 tacgtgctcg gatttgtcgt gctttggcag ggtcaaggcc ttcttgcaaa agcggcgaca
     9061 acatctgtac acgccaaact gcaattcctt ctgccttttt ttcgtccctc acgttaaaat
     9121 caccccttca tagagctttt aacatgagtt tacaccctgt ccttcaggac aagaatgcag
     9181 aacgggtatg cacccacata tttgaattaa ccaaaggacg gacaactcta gcaagccaac
     9241 cagattcgtc ccctacatag gtctttatcc tactcattgg ggatttatgg cctactactg
     9301 ttttcaatcc caatctaaca gcaatggcat caaggcttcc tgcaagatat cctgagaaat
     9361 cgctaaacca ttttcgaatg tggtatatgg tgctttcttc acaggagacc gtatcttcca
     9421 ttccgtcgag tattgcttcg atgctttcgc tggaatatct tttgtatgga actatgctat
     9481 caggcaattc atggtgtatt cgtctgcaac tcctgcaccg gagacggcgg attaccaaga
     9541 tgatcttttt tccgcagctt tttaatacct tacgggttcg agtgccgact acttttagct
     9601 ccagacttcc acataccgga caaatacttt tttccttgct ctttacgtga tatttgtctt
     9661 gagccttttt gtgcgggatt agttcatatt cctttacatt gatcatatct cacgactttc
     9721 cacagcactt tttgtatttc tttccactgc cacatgggca aggatcattc ctgcctgtct
     9781 tttctgtctt tatcggaccg gcagttgtgt gttttacaaa ggtgcttctt ttcagggatg
     9841 gaatctcctt ttttaactct gcgaccttct cattgtttcc aagagcttga tagatttcca
     9901 ccgctctctc aatgacatcc aaccggtccc gaagatcctt ttgttccaag gcctttgtaa
     9961 tgattgcttc tgcttttcta agatgtgctt ccgtcttgtt ttttccgaag tgatagcagt
    10021 ctgaccaacc aatatatccc cagccccagt ttgggtctgc ccccagccac atgctataca
    10081 ggcgatcgca ctcttcctca ttgccaagtt caaaatggga atctgctatc gctctgcgtg
    10141 tattctcaat tatcaggtca tcttcatctc cacatactgc aagcatttcc tcgcaatact
    10201 taatcctctt gcgaaagtat tccttgtttt caagccctgt gttatgtagt tccgcttcaa
    10261 gatcctgcac ataattggat aaaaactctg accattgata tttttgctgt agtttatcaa
    10321 tattgtcgat acctatgctg ctcattattg acttaatgtc gtcccatgcc tcaagccaca
    10381 tatcacaagc tttttgacta ttgcctcttt caagataatc atagccggaa acgatctttt
    10441 catctgcaat tttatatctt tccatatttt cctcccggat ggtgctgtta tttattccat
    10501 tctatcattt ttcccatgtc ttaaccatcc ggacactatt tccgtcaaaa ctcggacagg
    10561 caccactacc agctttatgc tattttactt tggctaaaac actatccatt gcaacagaca
    10621 tgtaaaagtt gtatatttaa aataaactcc gctccaaacc actttccctc aaacccttat
    10681 gcct
//
LOCUS       DAOSCC010000070        10568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4155_length_10568_cov_4.929040, whole genome shotgun sequence.
ACCESSION   DAOSCC010000070 DAOSCC010000000
VERSION     DAOSCC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_10568_cov_4.929040"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1725
                     /locus_tag="PLG72_05270"
     CDS             166..1725
                     /locus_tag="PLG72_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOJ80242.1"
                     /translation="MVEIAPPGDVAGILDVLNMHGYEAYAVGGCIRDAVMGREPKDWD
                     IATDARPEEVGRLFEKTVDTGIRHGTVTVVIGNRNYEVTTFRIDGRYTDSRHPEKVEF
                     TSRIGDDLRRRDFTVNAMAWNPQKGIIDPFGGMRDISGRIIRTVGKPSDRFGEDALRM
                     LRAIRFAATLGFEIEADTLEGIRQSSSLIRRISAERIREELTGILVSDDPMRFMLLMD
                     TGLLRHIMPELDSRLGPNSDRTGHDSLSETGKHILRSVAAAEADVCLRWAMLLHDAGR
                     CTDAENFEGLSDAHGSKDHKDYEECGRSGGPGAADALKTADTILGRLKFDNRNRSRIL
                     RIIRYLGMETEPERSAVARAVLEVGRDMFGDLLKAKRAHIAASMHRCGSIVSSVIPGV
                     SRRPDRMKSIKGGQYRDTDDLNTTTGRHYEDDGRNIAEDLYQLEKIETVFRSLLEGGY
                     CLSLKELKINGDDLKRLGFREGAEMGRMLDHLLDRVIDEPELNRKEALEKLALEYLHK
                     KRISSQQTGET"
     gene            1834..2604
                     /locus_tag="PLG72_05275"
     CDS             1834..2604
                     /locus_tag="PLG72_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOJ80243.1"
                     /translation="MSDLLKDKNIVIMGVRNKWSIAWAIATAARREGASLILTYLGER
                     EKKDVEVLAKELGGADTYQLDVASDADIDNFFESVKSRYGVVHGLVHAIAYAKAEDLQ
                     GRFVDTSRDGFALAMDVSAYSLIAVSRRAAGLMSEGGSIITLTYMGSEKVMPGYNVMG
                     LAKAALEAGVRYLADDLGPSGIRVNAISAGAVKTLSARGIKDFSSILEYTRQKAPLRK
                     NVDPEEIGNAAAFLLSDMSKGITGEILNVDCGMNIIGV"
     gene            complement(2833..4032)
                     /locus_tag="PLG72_05280"
     CDS             complement(2833..4032)
                     /locus_tag="PLG72_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ80244.1"
                     /translation="MDIFEKCFTYTDYKEVLAAGLYPYFHALETGQDTEVIIEGRKTV
                     MVGSNNYLGLTSDPRVKQAAIDAVKEFGSGCSGSRFLNGTLTLHLELEKKIAEFLNKE
                     AALTFSTGFQTNLGIITAIAGRNDYILCDSENHASIVDACRLSFAKTLKFEHNNMEDL
                     ERLLSKIDDKHGKLIVVDGVFSMEGDIADLPSIVKLARKYGARVMVDDAHSFGVLGEH
                     GRGTAEHFGLEDEVDIIMSTFSKSLASLGGFVAAKQDVIDYIKHNSRPFIFSASIPPA
                     NAAAAIAALEIIKSEPERIQRLRDISYYMRQGFRKLGLPIYEANSEITPIVPVMTYTN
                     EATLVVTKMLREEGVYVNPVLSPAVKPGLCRIRTSYTATHTQEQLDYTLEAFKRVFDK
                     LEITAGK"
     gene            4279..5217
                     /locus_tag="PLG72_05285"
     CDS             4279..5217
                     /locus_tag="PLG72_05285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ80245.1"
                     /translation="MRNHYGNIVLVTGASSGIGKSCAEYLAGLGFKVYGTSRKAPEPA
                     EAGLQDISGPSMNETPGDGAGRSMKNACGADGASKTACAARPGSVTMIRMDVCSEESV
                     KAAIDYVIEREGEIGIVVNNAGMGIAGSVEDTSTEEAFLQFDTNFFGMYRVIRQVLPH
                     MRKKGKGLIINISSVGALFPIPFQSMYVASKAAVELMSGSLRNELRNFGIKVCVVEPG
                     DTKTGFTGNRLFVKAGGEGSAYYHQSKESVAIMEKDEMNGPDPVIVAKAVARAIKRKS
                     PPAFITVGLGYKLLVFFKRLVPRSFESFVVAKMYKC"
     gene            complement(5457..5663)
                     /locus_tag="PLG72_05290"
     CDS             complement(5457..5663)
                     /locus_tag="PLG72_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ80246.1"
                     /translation="MDERIYLSTTNKKIAGVCGGIGEYFGIDPTVIRLLWVLTIFLDG
                     IGILAYFIAWIVMPRRSKCNYNNW"
     gene            5825..7744
                     /locus_tag="PLG72_05295"
     CDS             5825..7744
                     /locus_tag="PLG72_05295"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOJ80247.1"
                     /translation="MKRTACIFLTALVLFMSVSCSLRDDGIGVGPDRGQGKEMAGGTG
                     NGQDTPAETAEGISAGDAPAARQPSGIYPAKDNYLGDSKWGYIDSAGMFVIQPSFSQA
                     FRFQGNGLAVAGIGDRVGLIDRTGNFVADPVYTYINEFSEGRAVAVAPDGYVVLDGTG
                     SAISGKYEYIADYKSGRALYRTKTESGARLYGYLDEGGKPVIPAIYGDATSFEGNRAA
                     VKLNDGLYALIDRSGKAVKTLECWEISAFSDGKAAFRESADGRYGYVDTNGNVAIKPA
                     FVTAGSFRNHRAVVSVWGGGREIYGLIDENGRFVIIPQYEEIQMLGEERVALGIPRDP
                     DNLFTGLKYALADTEGNLLTDFVFCSVSQFSNGVASATDSTSTFFINTSGKKIENMPV
                     LEGSGQMEVLDGLIYANIDQRAYYMNKQGQPVYRPVSWVETESGVKVFEEKYKPNVNY
                     LVYYPVLGNLADLKVEANVNAKLREMWTDISTLSVKPSDILDYSYESNFQVVFSRKDL
                     LVLMESGYWYPFGAAHGTPVMNHAHIDIRTGRFYELDDLFKKDSNYEAVLTEIVGKQI
                     DELAAAEDSYIFADSFDGISSDQPFYITDKGLNLYFLPYEIAPYAAGFPTFMVTFDEI
                     SDIIDRNGDFWKSFN"
     gene            7841..9037
                     /locus_tag="PLG72_05300"
     CDS             7841..9037
                     /locus_tag="PLG72_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ80248.1"
                     /translation="MVRESFLRFIYQAASMQRWNDHIRPAKGFTELDKQAHKLYYAYV
                     LGRLEEETCPVDWMKMIEGALFEFFQRILLTDIKPPIFHMLMEKHGEQINRLVMKELR
                     DLGLGDIKQGFGERMETYLFDPDYSRHEKMILRASHYFVTNWEFNIVYQFSKGFYGIE
                     DTRRNIEKQLEQHQDLDCVRKLVFSHSLADFLNLIGQLRFQQRWAQTQRIPETSVAGH
                     MLIVALLSYILTLEMDACEKRKYNNFFAGLFHDLPEVLTRDIISPVKNSVDGLSDLIK
                     SIEEEQMEKMLLPLLPEKWRDEIRYFTDDEFSCRIILDGKTIKVSTEKINELYNSDVF
                     SPVDGSITEFCDKFAAYMEAYLSIKHGITSKNLVDGHADLYRRFARTKIAGIDMGVIF
                     DYFRLD"
     gene            9064..9717
                     /locus_tag="PLG72_05305"
     CDS             9064..9717
                     /locus_tag="PLG72_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80249.1"
                     /translation="MNKYMGFYELKALNIPSVPWELFTNDTVLDEGVLWTVRVATAAG
                     NDLNLPRAVGVDHAEARMKGREFLREYGEKGIVVYYPYFIAEKSGVLDINSQRTVIEA
                     VDRDLWNLVTFGKKNVTIIAPSDKIGHISDVSCNEAGSWPIDGVQITGDGSFLSPEEI
                     KELVRYGAVIRGRFRDEISGGSSILAEWSYAYSTDVSMKPIGPRYLVFYELRDLSLH"
     gene            9980..>10568
                     /locus_tag="PLG72_05310"
     CDS             9980..>10568
                     /locus_tag="PLG72_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOJ80250.1"
                     /translation="MEREKADNVNEPESMKKKRAGRHLRLSEYITSQLPEGAVVDFIF
                     YEDFDGDGEKEAVIGVTRFAPFPPDSAVLIVKAEKNGDITHDWFMFRESPHGSEQAGV
                     IDSAAAADTDGDGLKEVVISRVFGHKHEIDITVIDWEGAAPHQVWHSGQAYFHGSLQV
                     ADTDGDGIAEIVVEFGTQDGHEVISAEEACYHVRES"
BASE COUNT         2761 a         2394 c         3007 g         2406 t
ORIGIN      
        1 gtaaggggcc cggttattgc ccaaaagctg taaaaccgta caaaatggaa ttcgggcaga
       61 aaggacagac agtatataat cttcatcggg atgatgttat aatcttaagt gtgaccggaa
      121 catcacggca tttggcaaaa cgcatactac gcgtgaggtg atacagtggt tgaaatcgct
      181 ccgcccgggg acgtggctgg aatacttgat gtactgaata tgcacgggta cgaagcatat
      241 gcggtcggcg gctgcattcg tgacgccgtg atgggcaggg agccgaaaga ctgggatata
      301 gcgactgatg cgcgccctga agaagtcggc aggctctttg agaaaacagt ggacaccggg
      361 atcaggcacg gcactgttac ggtagtgatc ggaaacagga actatgaagt gaccacattc
      421 cgcatagacg gcaggtacac tgacagcagg cacccggaga aggtggagtt cacatcaagg
      481 atcggggatg acctgcgcag gagagatttt actgtaaacg caatggcatg gaatccccaa
      541 aaaggcatca tcgatccttt cggaggtatg agggacattt ccggcaggat catacggacc
      601 gttggcaaac cgtcggacag gttcggggaa gatgccctgc gcatgctgcg ggccataagg
      661 ttcgcagcga cacttggttt tgagatcgaa gcggacactc ttgaagggat aaggcagagc
      721 agcagcctca tccgcaggat cagcgccgag aggatcaggg aagaactgac ggggatcctg
      781 gtttcggatg atccgatgag attcatgctg ctcatggata caggactgct ccggcatata
      841 atgcccgaac tggattcgcg gctcggtccc aatagcgatc gtaccggcca cgacagcctt
      901 tcggaaacgg gaaaacacat cctgcgttcc gttgcggccg cggaagcgga tgtgtgcctg
      961 cgatgggcga tgcttctgca tgatgcaggc agatgtacag acgctgaaaa ttttgaaggc
     1021 ttaagtgacg cacacggctc taaggatcat aaagactatg aagaatgtgg aagatctgga
     1081 ggtcctggag cggcagatgc attgaaaact gcagatacca tactgggccg gcttaagttt
     1141 gataatagga acaggagcag gatactgagg atcatcaggt acctcggtat ggaaacagag
     1201 cctgagagga gcgctgttgc aagagctgtg cttgaagtcg gcagagatat gtttggggat
     1261 cttctgaaag ccaaaagagc tcatatagca gcttcaatgc atcgctgcgg cagcatcgtc
     1321 agcagcgtca ttccgggagt atcgcgtcgg cctgaccgga tgaaaagcat aaaaggaggt
     1381 caataccgtg atacggatga cctgaatacc acaactggac ggcattacga ggatgatggc
     1441 cggaatatcg ctgaagatct gtatcagctg gaaaagatcg aaaccgtctt caggtcgctg
     1501 ctggaaggag gatactgtct cagcctgaaa gaactgaaaa taaacggtga cgaccttaag
     1561 cggctgggat tccgtgaagg cgccgaaatg ggacggatgt tggatcatct gctggacagg
     1621 gtcatagatg aaccggaact caacaggaag gaggcacttg aaaaactggc gctggaatac
     1681 ctgcataaaa agcggatttc ttcgcagcag accggtgaaa catgaccgca ggcactgaag
     1741 aaataacagg tataaatgca gcgtatatat aaatctgtta caatgttata atactgtacc
     1801 ataatacata tacagcagga ggaagtgttt tggatgagtg atttgctgaa ggataaaaac
     1861 atcgtaataa tgggcgtgag gaacaaatgg agcatagcct gggcaatagc tactgcggcc
     1921 cgaagggaag gggcgtcgct gatactgacg tacctgggcg aaagggagaa aaaagacgtt
     1981 gaagtcctgg caaaggaact tggcggtgct gacacttacc agctcgatgt ggcatccgac
     2041 gccgatatcg ataatttctt cgaatcagtg aaaagcaggt atggggtggt ccacggactc
     2101 gtgcatgcta tagcatacgc caaagcggaa gacctccagg gaagattcgt ggacacgtca
     2161 cgggacgggt tcgcgctcgc catggatgtc agtgcatact cgctcatcgc agtcagcagg
     2221 agggccgccg ggctcatgag tgaaggaggc agtataataa cgctgactta tatggggtcg
     2281 gaaaaggtca tgccgggcta taatgtgatg ggattggcaa aagctgcact cgaagccggg
     2341 gtcagatacc ttgctgatga tctcgggcct tcggggataa gagtgaacgc tatatccgcg
     2401 ggagctgtga agacgctgtc agcgcgaggt atcaaggatt tcagcagtat ccttgaatat
     2461 acccggcaga aagcaccgct caggaaaaac gtcgatccgg aagagatagg aaatgccgcg
     2521 gcattcctgc tcagcgacat gtcgaaagga ataacgggcg agatcctgaa tgtcgactgc
     2581 ggcatgaata ttattggagt gtaaacagtt atcgatgtgt aagacaaccg ctatagttat
     2641 ataagtatta ctggtacgta aataccagcg tgatgcaagc tttataacag gtgtacaaaa
     2701 cgacaggctg ccttcaacgg cagcctgtcg agtatatgca ttatttgttt ttccgtatct
     2761 tctgttatat tgcctgaaat attgcttttt gttttttgaa ccggttacag ctgccttacc
     2821 cgctgccgtt cattatttgc ctgccgtgat ctccagttta tcgaaaaccc tcttgaatgc
     2881 ttcaagagta taatcaagct gctcctgcgt atgggttgcc gtatagctgg tcctgatcct
     2941 gcagaggccg ggcttcactg ccggcgacag tacagggttc acatagacgc cttcttcgcg
     3001 cagcatcttg gtaacgacga gagtagcctc attggtataa gtcatgacag gcacgatcgg
     3061 cgtgatctcg ctgttggctt catatatggg caggccgagt ttcctgaagc cctgcctcat
     3121 gtagtaggat atgtctctga gcctttggat gcgttcaggt tcggatttta ttatctcaag
     3181 tgcggcaatc gcagcagccg cgtttgccgg cggtatggat gcggagaata tgaacggcct
     3241 ggaattatgc ttgatatagt ctatgacgtc ctgctttgcc gctacgaaac cgccgaggct
     3301 tgccaaagat ttactgaagg tactcattat gatatcgact tcgtcttcca gcccgaaatg
     3361 ttcggctgtg cctcttccat gctcgccaag cactccgaat gagtgggcgt cgtcgaccat
     3421 gaccctggcg ccatatttcc ttgccagttt cacaatggac ggcaggtctg caatatctcc
     3481 ctccatgctg aacacaccgt ctactacgat gagttttcca tgtttgtcat ctattttgga
     3541 cagaagcctt tccaggtctt ccatattgtt gtgttcgaat ttgagagttt ttgcaaaact
     3601 gagtctgcat gcgtcgacta tgcttgcgtg gttttcgctg tcgcacagga tgtaatcgtt
     3661 cctgcccgcg atagcggtta tgatgcccag attggtctga aaaccggtgc tgaaagtgag
     3721 cgctgcttcc ttgttgagga attcggcgat ctttttctcc aactcaaggt ggagagtcag
     3781 tgtcccgttg aggaacctcg atcccgagca tcccgatccg aactctttca ctgcgtcgat
     3841 cgcggcctgt ttgaccctgg ggtcggaggt aagccccaaa tagttgttgg aacctaccat
     3901 tacagttttt cttccttcga tgatgacttc agtgtcctgt ccggtctcaa gggcgtggaa
     3961 atacgggtaa aggccggctg ccaaaacctc cttgtaatcg gtgtaggtaa agcatttttc
     4021 aaagatatcc ataatcaaat gatctcccct tcacgatttg agtatcttag gtaatgattc
     4081 cgggctgcaa ccattttacc acataaaata caataagtat atatttatga tacatctatt
     4141 tatcacgaaa agccgccttt taccggaaaa aacgagccgg gccgggcaat tacgcccggt
     4201 tttcttcatt acggcagttt gatatggtat aattagttac cggaaatacc ggcagtccgc
     4261 cggatcgggg aggtcgaaat gagaaatcat tacggaaaca tcgtacttgt caccggagcg
     4321 tcatccggta ttggtaaaag ctgtgcggaa tatcttgcgg gattgggttt caaagtctac
     4381 ggcacatcga ggaaggcacc ggagcctgcg gaggccggtt tgcaggatat ttccggtccc
     4441 agtatgaatg aaactccggg ggacggcgcc ggcaggtcca tgaaaaatgc ctgcggtgca
     4501 gatggtgcct caaaaactgc atgtgctgcc cggccgggca gtgtgacgat gatccgcatg
     4561 gatgtctgca gcgaggagtc tgtaaaggct gcgatcgatt atgtcattga acgggaaggc
     4621 gagataggga tcgtcgtgaa caatgcagga atgggcatag ccgggtccgt cgaggacact
     4681 tcaacggagg aagcgttcct gcagttcgat accaatttct tcggtatgta cagggtaata
     4741 cggcaggtcc tcccgcatat gaggaagaag ggaaaaggcc tgataataaa tatttcctcg
     4801 gttggcgcac tgttcccgat accgttccag tccatgtacg tagcatcaaa ggcggcggtc
     4861 gagctcatga gcggttcact ccgcaatgaa ctcaggaatt tcggtataaa agtctgcgtt
     4921 gtggaacccg gcgatacgaa aaccggcttt acgggcaacc ggttgtttgt gaaggcgggt
     4981 ggcgaaggct ccgcatatta tcatcaaagc aaagagtctg tggcaataat ggaaaaggac
     5041 gagatgaacg ggccagatcc cgttattgtc gcaaaagcgg tcgccagggc gataaagcgg
     5101 aagagtcctc cggctttcat aaccgtagga ttgggctaca aactgctggt gtttttcaaa
     5161 aggcttgtcc ccaggtcatt cgaatctttc gtcgtggcaa agatgtacaa gtgctgaaaa
     5221 agcggcagtt gtacgcgaga cagtctgtga tccatgtcca gggatccatc cgggtatctc
     5281 cgctggacag gtaaaatatt cttcccattt taccagacaa tagcgaagta caacatggtg
     5341 gaatcatcct gcgcccgcaa agtccaggca gaggctcata ttacgggccg atgcgtcagc
     5401 cgccggccgg cgtgccaata actgaccggc atgcctgtcg gcggctgccg ttcttgttac
     5461 cagttgttgt aattacattt gctgcggcga ggcataacga tccatgctat gaagtaggca
     5521 agaatgccga taccgtcgag gaatatggta aggacccaca ggagccttat aacggtggga
     5581 tcgatcccga aatattcccc aatgcccccg cacacgccgg cgattttttt atttgtagtt
     5641 gacaggtata tcctttcatc catatcgcat gctccttttc tctcatgaac ttgatgttgc
     5701 cttttctata gaatcatacg gaacgggtgc ctgtgtgtct gataagtttt taccggatac
     5761 ttttatcagg agagttttga taatataata gtatcggata atattcaagg gggatcattt
     5821 atacatgaaa agaacagcct gcatcttcct gactgcattg gtgctgttca tgtccgtatc
     5881 atgcagcctg cgggatgacg gcattggcgt ggggccggac agaggacagg gaaaggaaat
     5941 ggccggtggg accggcaacg ggcaggatac gcctgccgaa acggcagaag gcatttcagc
     6001 cggtgatgca ccggcggccc ggcagccatc cggcatttat ccggcgaaag ataattatct
     6061 aggcgattcg aaatggggtt acatagacag tgccggcatg ttcgtgatac agccttcgtt
     6121 ttcacaggca ttcaggttcc agggcaacgg gctggctgtt gcagggatcg gcgaccgtgt
     6181 cgggctcatc gaccgtaccg ggaatttcgt tgccgacccg gtctatacat atataaatga
     6241 attcagcgag gggcgcgccg ttgctgtggc ccctgacgga tatgtggtgc tggacgggac
     6301 cggcagcgcc atatccggta aatatgaata tattgccgac tacaaaagcg gcagggcgct
     6361 ttaccgtacg aagaccgaat cgggcgcgag actttacggc taccttgatg aaggcggaaa
     6421 gccggtcata cccgcgattt acggcgacgc gaccagcttt gaagggaaca gggcggcggt
     6481 aaaactgaat gatggtctgt acgctctgat cgacaggtca ggaaaggctg taaaaacact
     6541 ggaatgctgg gaaatatccg ctttttccga cggcaaggca gctttcaggg aaagcgccga
     6601 tggcagatac ggctatgtcg acaccaacgg gaatgtagcc ataaagccgg ctttcgtcac
     6661 tgcaggaagc ttcaggaacc accgcgcggt cgtctccgtg tggggaggag gcagggagat
     6721 atacggcctt atcgatgaga acggcaggtt cgttattata ccccagtatg aagagataca
     6781 gatgcttgga gaggaaaggg tggcgcttgg gatcccgaga gatcccgaca atctctttac
     6841 aggtctgaaa tatgcgctgg cggacactga aggaaacctg ctcacggatt ttgttttctg
     6901 cagcgtcagc cagttcagca atggggtcgc ttccgcaact gacagtactt ctacgttctt
     6961 cataaacacc tcaggcaaga agatcgaaaa catgcctgtg ctggaagggt caggccagat
     7021 ggaggtattg gacgggctca tatatgcaaa tatcgatcaa agggcttatt acatgaacaa
     7081 gcagggccag cccgtataca ggcctgtatc ctgggtcgaa acggaaagcg gagtaaaggt
     7141 gttcgaagag aagtacaaac cgaatgtcaa ttacctggta tattaccctg tgctcggcaa
     7201 cctggccgac ctgaaggtgg aagccaatgt caacgcgaag ctcagggaga tgtggactga
     7261 tatctcaacg ttgtcagtca aaccttccga tatactcgat tacagctatg aaagcaattt
     7321 ccaggtcgtg ttcagccgca aggatctgct ggtactgatg gaatcggggt actggtatcc
     7381 gttcggggca gcccatggta cgcctgtaat gaaccatgcg catatagata tccggacggg
     7441 caggttctat gaacttgacg atctgtttaa aaaggacagc aattacgagg ctgtgctgac
     7501 agagatcgta ggaaagcaga tagatgaact tgccgcggca gaagattcgt acatctttgc
     7561 tgattcattt gatggcataa gcagcgatca gccgttctac ataaccgaca aggggctgaa
     7621 cctgtatttc ctgccgtatg agatagcgcc gtatgcggcg ggattcccga cattcatggt
     7681 aacgttcgat gagatttcgg acataatcga caggaacggg gacttctgga agtcgttcaa
     7741 ctgacgcaaa gaagttttac atatatctgt ttgacgaacc ggcactggag agccgcaaga
     7801 ccggcacagt actgcggcac ggacagggag gcataagacg atggtcagag agagcttttt
     7861 gagattcata taccaggctg caagcatgca gcggtggaac gatcacatca ggcctgccaa
     7921 aggctttacc gaactggaca agcaggcgca taagctttac tacgcctatg tcctcggcag
     7981 gctcgaggaa gagacttgtc cggtagactg gatgaagatg atcgaaggcg cactgttcga
     8041 atttttccag aggatcctgc tgacagatat aaaacctcct attttccaca tgctcatgga
     8101 aaaacacggc gaacagataa acaggctcgt gatgaaagaa ctccgtgatc ttggattggg
     8161 cgacataaaa cagggcttcg gggaaaggat ggaaacatac ctcttcgatc cggactacag
     8221 ccggcatgaa aaaatgatac tgagagcatc ccattatttc gtgacgaact gggaattcaa
     8281 cattgtgtac cagttcagca aaggttttta tgggatagag gatacgagaa ggaacataga
     8341 gaagcaactg gagcagcacc aggacctgga ttgcgttcga aaactggttt tcagccacag
     8401 tctcgcggac ttcctgaatc tgatcgggca gctcaggttc cagcaaaggt gggcgcagac
     8461 ccagaggata ccggagacgt cggtcgccgg ccatatgctg atcgttgcgc tgctttccta
     8521 tatcctgaca cttgagatgg atgcgtgtga aaaacggaaa tacaacaatt tctttgcagg
     8581 actgttccac gatctgcccg aagttctgac aagggatata atttcgcctg tcaagaattc
     8641 tgtcgacggg ctgagcgatc tgatcaagtc catcgaggaa gaacagatgg agaagatgct
     8701 gcttccgctg ctgcctgaga aatggaggga cgagatccgc tatttcaccg atgacgagtt
     8761 ttcatgcagg atcatccttg acggcaaaac cataaaggtg agcactgaaa aaattaacga
     8821 actttacaac agcgacgtat tctcaccggt cgacgggagc ataaccgagt tctgcgacaa
     8881 gtttgcggca tatatggaag cctacctctc cataaagcac gggatcactt caaagaacct
     8941 ggtcgacgga cacgcggacc tgtacaggag atttgcccgg acaaagatag ccgggatcga
     9001 tatgggcgtc atttttgact acttcaggct ggattgaccg aacctgacaa tcggaggact
     9061 gctgtgaaca aatacatggg attctatgaa ctgaaggcat tgaacatccc gtcggtaccg
     9121 tgggaacttt ttacgaatga tacggtgctt gacgaaggag tgctctggac ggtcagggtc
     9181 gcaactgctg cggggaatga cctgaacctg ccaagggcgg tcggagtgga ccatgccgaa
     9241 gcgcggatga agggcaggga gtttttgcgg gaatacgggg agaaaggaat cgtcgtgtac
     9301 tatccctatt ttatagctga aaagagcggc gttctcgata taaacagcca gaggaccgtg
     9361 atcgaggccg tggaccgcga tctgtggaat ctcgtgacat ttgggaagaa aaatgtgact
     9421 ataatcgccc cttcagataa aatcggccat ataagtgatg tatcctgcaa cgaggccggc
     9481 agttggccca ttgacggcgt acagataaca ggagacggca gtttcctgtc accggaagag
     9541 ataaaagaac ttgtgagata tggcgctgtc atcaggggca ggttcaggga tgagataagc
     9601 ggaggttcct ccatacttgc tgaatggagc tacgcttaca gcaccgatgt cagcatgaaa
     9661 cccatcgggc ccaggtatct tgtcttttat gaactcaggg acctttcgct gcattgaggc
     9721 ttccgcgcat taagacccgc atctgactga ctttcatcat gcctgcggta ccggttcgcg
     9781 cactgaggct ccgttttgct gacaaatagc tccaggcagg tttccgcgat ccatacatct
     9841 gaatatattg ataatggcag ccaatttgcc ggctgacccg ggcatcatgc agatatctga
     9901 aagggattgc ccggagttat gcccggcggg tcatcatgca cggctgccca tgacgcctga
     9961 ctggatcggt gaggatgata tggaaaggga aaaagcagat aacgtaaatg agccggagag
    10021 catgaaaaag aagagagccg gcaggcatct ccggctgtct gagtatataa cgtcacagct
    10081 gccggagggc gctgtggtgg attttatttt ttatgaagat tttgacggag acggggaaaa
    10141 ggaagcagtg ataggcgtga cccgatttgc gccgttccct cctgattccg cggtgttgat
    10201 agtaaaagcg gagaagaacg gcgatataac gcatgactgg ttcatgttcc gggaatcgcc
    10261 gcacggatcc gagcaggccg gtgtcatcga cagcgcggca gcggcggaca ccgatggcga
    10321 cgggctgaag gaagtcgtga tatcgagggt gttcggccac aaacatgaaa tagatataac
    10381 tgtgatcgac tgggaaggcg cggctccgca ccaggtatgg cacagcgggc aggcctattt
    10441 ccacggcagc cttcaggttg cagatactga cggcgacggt attgccgaga tcgtcgtgga
    10501 atttggaaca caggacggac acgaggtgat atcggccgag gaagcctgct accatgtcag
    10561 ggagagca
//
LOCUS       DAOSCC010000071        10494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4191_length_10494_cov_4.574289, whole genome shotgun sequence.
ACCESSION   DAOSCC010000071 DAOSCC010000000
VERSION     DAOSCC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_10494_cov_4.574289"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLG72_05315"
     CDS             <1..880
                     /locus_tag="PLG72_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ80251.1"
                     /translation="TDDCITNIPDCCMRAGSDVNWHIACGTPVPRLGSIPVCFRKASR
                     LLSYRYICPDEHILTEEVISNISSRQNSGQYGGKNGIDQECIVNFLGNGSMEDIDRYA
                     DQLMQSAKAEAAMPTMLYRYLAMTVYLAAVKYLDTIGCRSYGIWSQELRPDDGISAPE
                     EAHEYMCRVMRHVLELRDDASKKQQQDLLNQAIEYINTHYHEESISLDKVAKEVNVSP
                     NYFSAIFSQEVGMTFIEYLTNKRIERAKYLLRHTDRRSGEIAFSVGYRDPHYFSFVFK
                     KVTGITPSEYRKGVDQ"
     gene            877..2325
                     /locus_tag="PLG72_05320"
     CDS             877..2325
                     /locus_tag="PLG72_05320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ80252.1"
                     /translation="MKTGLHRKTKNGKITMQAKLWKMILIVSVPLMIMIIVIASIGIG
                     YALQYKAILNNVTMASEFNQNFKDDVDLKMYYYVIDSQLSEGLPLKEVQSAEMIARKL
                     TETTTKKESLRAINSVLNLCHNLQDKMEKIAETKEYNSREEQLVNNIYILTDMIQEYM
                     YTYLYYEAAHLDALQKTMIRNMIVLMTVSVVLSAILLIFLLRKSRKLSNKIINPIDKI
                     CERLVAIGKGDLLVCEPIQADVREVQLLTDGIETMVGQLRRLIEKTAEQEKQRRRTEL
                     ALLQAQINPHFLYNTLDTIIWLIESGEIPEAVTMVSTLSNFFRSSLSRGKNVITLAEE
                     EQHIRSYLEIQKMRYRDLMEYEIDIPDHLKFYILPKLTLQPLVENALYHGIKNRRRKG
                     CIRVTGRERNGCIQLEVADDGAGMTPERLEEVRASLEEAGGIESFGLKAVHRRIRILF
                     GADYGLALESEPDAGTKVIVTIPMQTAEESAS"
     gene            2389..3384
                     /locus_tag="PLG72_05325"
     CDS             2389..3384
                     /locus_tag="PLG72_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ80253.1"
                     /translation="MSKWKLAAVACLMAILGGCVMRTQQATSPDEENLIVVGFSQVGA
                     ESEWRVTNTESIKSALSEENGYVLIFEDARQEQENQIRAIRSFIQQDVDYIVFSPKLE
                     TGWKTVLQEAKDAGIPVIMIDRDVEEESRKLLTAYIGSDFYKEGKKAVVWLEDFLEKK
                     GRTEEPLRIVHIQGTIGSSAQIGRTRAMEEAVARHPNWELVAQECGDFTRAKTYEVMS
                     GILEKTKDIDVVYCENDGEALGAIDAMEEAGLKCDPEDGVIVISFDATRLALQYCLEG
                     KIALNVECNPLQGPYVDEVIKRLERGETIEREIYVEETQFDYLTITQEMIDSRKY"
     gene            3800..4276
                     /locus_tag="PLG72_05330"
     CDS             3800..4276
                     /locus_tag="PLG72_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ80254.1"
                     /translation="MRTSEELLEIYNRHVDTVYRVCFMFMKNVPDTEDMVQNTFIRLM
                     KDKTVFRDEEHEKAWLIRTASNLCKDHFRHWWSKTVGIDQAGETAVDQDFNIDSSLEK
                     VLALPSKIRTAVYLYYYEGYSTVEIAKILGKNESTVRGYLHTGRKLLKMEMEGGLE"
     gene            4273..5469
                     /locus_tag="PLG72_05335"
     CDS             4273..5469
                     /locus_tag="PLG72_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80255.1"
                     /translation="MKKEDLRNSFDLIKPDENAKKRMLDNIMRSAGKKDGSRRMYFVK
                     AIPAFALTVVVAAGVLMYSLKPGNNGSPPGQVTDLAGGIGREDAAAPLLDQFRVDDRH
                     YILLTDELRNDFGLPSEIKEGDIGEKLTDITVSPDESLTGREVYRYVPAGCEAVVAVK
                     GDDGYRLFRFFTFESYNSNQDEDAVEYLELYGINSAEDISKVLLIGHSEQGKMSGVDD
                     IREITDRSEIEQFYGYYSVLKNNSDKYFDRLFNYNGTGTGNSSVGVDIAERERMAAAE
                     PAGPIEPDAAGFGSDMPMEIAPAAPDTPVSANVPAPADAPVPQKPSATADPSAGIVTD
                     MGGGTTGSVQGSTGSTGDALDLPITIRIYNRNGIYFDSVYYRNIGFINRYEVSDEFAA
                     FLSARF"
     gene            5783..6685
                     /locus_tag="PLG72_05340"
     CDS             5783..6685
                     /locus_tag="PLG72_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ80256.1"
                     /translation="MLKYIVPEYKRLRVIVDTDAACEADDPFAIAHALMCRKFDVKAI
                     LAEQFGGPETTKKSYDEIMTVLDAMDISVPVFMGEEGKLSDIPGDDISPASKFLAEEA
                     MRDDDRPLFVLCLGAVTNVASAIRRAPGIIPRMTVVWIGGQGYDSYTPDFREFNSGND
                     IDAANLVVSSGVELWQIPSDVYGSMRISLAEIQHRIYPCGKIGKHLFENMVDYNMSKH
                     AGWTAGESWALGDNPAVGVAMDQNCGRFIYREAPIFNEDTTYTFEAGRPKIRVYTSID
                     PRFILEDLISKLQLIYGAGHDRQT"
     gene            6817..8544
                     /locus_tag="PLG72_05345"
     CDS             6817..8544
                     /locus_tag="PLG72_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ80257.1"
                     /translation="MPGNGFEQPYRHVVSGTVTGRYEVPDCPEWAHSAVFYQIYPQSF
                     YDSNGDGIGDLQGIIQKLDYVKSLGADAIWINPFFESPFNDAGYDISDYYKVAPRYGT
                     NEDARMLFEEAHKRGIKVLFDFVASYTSIEHEWFRESCRQEKNRYSNWYIWTDGTWAP
                     MPEEQKDRFIQGYARRDGQFMRNFYWSQPALNYGYEHPDPAFPWQLPTDHPDVLAMRE
                     EMKNVVRYWMDLGADGFRADMAGALVKSTRYRGNSQFYANKASGTQKFWQEVREILDR
                     EYPGSFMVAEWSHPISAIEGGFHADFYHWFSGFNDLFQKESWRILNGLSEGHSFFDAE
                     GKGNITDFLAGYMEQYEAVKGKGFVCIPVGNHDIARINNNRSPKELEMIFAFSITLPG
                     MPFIYYGNEIGMRQLHGLPHVEGAYKPRAGARTPMQWKKGPNAGFSAAPAEKLYLPVD
                     PADDYPNVEEQEKNPDSLLNRVRKLISLRKRYKALSAHAEFVPLYAVENGYPFIYARA
                     ADEEVILVVLNPSGQERKAEFTSNLEISGRKLIAGEEIRMDSDGSKISVAVPPITYSV
                     YIMNARHWS"
     gene            8548..9534
                     /locus_tag="PLG72_05350"
     CDS             8548..9534
                     /locus_tag="PLG72_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOJ80258.1"
                     /translation="MITLSNRQTRQFLLLRHGLLGEHRFAGKHGALDFVRQAGCIQFD
                     PVDVCGKNAELTLQSRIKGFTKNTLYELLYVDRKLVDYPDKNLSIIPAEDWPYFERYR
                     QAAIAGGRRFKELAGLADQAKAYIREHGPVCSDDLPITGGIRWHSSIHWSGNWTGITK
                     AARSVLEHLYSTGELVIHHKNGSRKFYDLAERHIPPEILYAPDPLPDEFQHKKWRILR
                     RIGAVGLLWDRPSDAWLNIWGLNAREIYTPADKRKYGHYVLPLLYGENFVGRVEAAAD
                     KKTCTLILRNIWYEGNVKQTKRLADAIWKCINRFAEFNECSAVKSECALMKD"
     gene            9860..>10494
                     /locus_tag="PLG72_05355"
     CDS             9860..>10494
                     /locus_tag="PLG72_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80259.1"
                     /translation="MSFDELLVFISSCIYSGYSMIRWYNKLPSYGPVKRDKAVKLVLG
                     LLPVISFFIIVYTLRVLASFDVVNDFVYILFYIALGYAWVFLGMRWVFRFFDISWIDD
                     VLLLNNKAALITVAGAFLGLVTIYSGANIGDGPGWWCVIFAGGLGLVSWFLLGRLVNK
                     FAQVFERITVDRDIYCGIRTGAYLLAGGIILARASAGDWTSFSKTIVEFMVG"
BASE COUNT         2786 a         2429 c         2966 g         2313 t
ORIGIN      
        1 gaccgacgat tgcatcacga acatccccga ctgctgcatg agggcgggca gtgatgttaa
       61 ctggcatata gcctgcggta cccctgtgcc ccgtcttgga agcatccccg tatgtttccg
      121 gaaggcgagc cgtctgctgt cctatcggta catatgccct gatgaacaca tcctgacgga
      181 ggaagtcatc agcaatatca gcagcaggca aaattcaggg cagtacggag gcaaaaacgg
      241 gatcgaccag gagtgtatag tgaatttcct cggcaacgga tccatggaag atatcgaccg
      301 ctatgccgat caactgatgc aaagcgcaaa agccgaagca gcaatgccta cgatgttgta
      361 caggtacctt gccatgacgg tctacctggc cgccgtgaaa tacctggata cgataggctg
      421 ccgttcgtac ggcatctggt cgcaggaact ccgccccgac gacggcatat ccgcgccgga
      481 agaggcacat gagtatatgt gcagggtgat gcggcatgtc ctggaactgc gtgacgacgc
      541 aagcaaaaaa cagcagcagg acctgctgaa ccaggctata gaatatatca acacacatta
      601 tcatgaggag tccatatcac tcgataaagt ggcgaaggaa gtcaatgtca gcccgaacta
      661 cttctcggca atattcagcc aggaagtcgg gatgaccttt atcgaatacc tcacgaacaa
      721 gcggatcgaa cgggcgaaat acctgctcag gcataccgac cggcgctcag gcgagatcgc
      781 attttcggtg ggatacaggg accctcatta tttcagcttt gtttttaaaa aagtaacagg
      841 gatcacgccc agtgaatatc gaaagggagt agaccagtga aaacgggttt gcaccggaaa
      901 acaaaaaacg ggaaaatcac catgcaggcc aagctgtgga agatgatcct catcgtctca
      961 gtcccgctga tgatcatgat catcgtgatc gcttccatag ggatagggta tgcgctgcag
     1021 tataaagcca tactgaacaa tgtgacgatg gcttccgaat tcaaccagaa tttcaaggac
     1081 gatgtggacc tgaaaatgta ctattacgtc attgacagcc agttatcgga aggcctgccc
     1141 ctcaaggaag tccagtctgc cgagatgata gcgcggaaac tgaccgaaac caccacgaag
     1201 aaagagagcc tgcgcgccat caacagcgtg ctcaaccttt gccacaacct tcaggacaaa
     1261 atggaaaaga tcgcggagac caaggaatat aactcacgcg aggagcagct ggtgaacaat
     1321 atttacatac tgaccgacat gatacaggaa tacatgtaca cttatctgta ttacgaagct
     1381 gcgcacctgg acgctttgca gaagaccatg atcaggaaca tgatcgtcct tatgacggtg
     1441 agcgtcgttt tgtcggccat cctgctgatc ttcctgctga ggaaatcgag aaagctcagc
     1501 aacaagatca tcaacccgat agacaagata tgtgagcggc ttgttgcgat cgggaaaggc
     1561 gacctgctcg tttgcgagcc gatacaggct gatgtcaggg aagtacagct gctcaccgac
     1621 ggtatcgaga ccatggtcgg gcagctgaga cggctgatag aaaagacggc cgagcaggaa
     1681 aaacagcgcc ggcgtaccga acttgcgctg ctgcaggcgc agatcaatcc gcatttcctt
     1741 tacaacacac tcgacactat aatatggctg atcgaatcag gagagatccc ggaggctgtc
     1801 actatggtca gcacgctgtc caacttcttc cggtcctccc tgagccgggg caaaaacgtg
     1861 atcacgcttg ccgaggagga acagcatatc cgcagttatc ttgagatcca aaagatgcgt
     1921 taccgggatc tgatggaata tgagatcgat atccccgatc atctgaaatt ttacatactt
     1981 ccaaaactca cgctccagcc gctggtggaa aatgcactct atcacgggat aaaaaacagg
     2041 cgccggaaag gctgtatcag ggtgacaggc agggaacgga acggatgtat ccagttggaa
     2101 gtggctgacg acggagcggg gatgacgccg gaaagactgg aagaggtgcg ggcgtccctt
     2161 gaagaagccg gcgggatcga aagtttcgga ctaaaggcag tgcaccggag gataaggatc
     2221 ctgtttggcg ctgattatgg actggctttg gaaagcgagc cggatgcggg tacgaaggtc
     2281 attgtaacta tcccgatgca gactgccgaa gaatccgcgt catgaaacgg acggtattcg
     2341 ccgttcgatc cattgatgca ggagaggtga agagaaggag agatgaagat gagcaaatgg
     2401 aagcttgcgg cagttgcctg cctgatggcc atccttggcg gatgtgtcat gagaacgcag
     2461 caggcaacgt ctcctgatga ggaaaacctt atcgtggtcg gattttcgca ggtcggggca
     2521 gaatcggaat ggcgggtcac caacacggaa agcatcaaat cggcattgag cgaagaaaac
     2581 ggatatgttt tgattttcga agatgctcgc caggaacagg agaaccagat ccgggcgatc
     2641 cggagcttca tacagcagga cgttgattat atcgtgtttt cacctaagct ggaaaccggc
     2701 tggaaaacgg tgctgcagga ggcaaaagac gctgggatac ccgtgatcat gatagaccgt
     2761 gacgtggagg aagaaagcag gaagctgctg actgcgtata tcgggtcaga tttctataaa
     2821 gaaggaaaga aagccgttgt atggctggaa gacttcctgg aaaagaaggg gaggaccgag
     2881 gaacctttaa ggatcgtcca catacaggga acgatcggtt cttcggcaca gataggccgg
     2941 acccgagcca tggaagaggc ggtcgcaagg catccgaact gggaactggt tgcacaggaa
     3001 tgcggcgact tcacacgtgc aaagacatac gaggtaatga gcgggatatt ggaaaagacc
     3061 aaggatattg atgtggtata ctgtgaaaat gacggtgagg ctctcggcgc catcgatgcg
     3121 atggaggaag cgggactgaa atgtgatccg gaggacggag tgatcgtcat atcgttcgat
     3181 gcgaccaggc tggcactcca atactgcctg gaaggcaaga tcgcgctgaa tgtggaatgc
     3241 aacccgctgc agggccctta tgtcgatgaa gtcataaaac ggctggaacg cggtgagacc
     3301 atagagcgtg aaatatatgt cgaagagacc cagttcgatt atctgacaat cacccaggaa
     3361 atgatcgaca gcaggaaata ctgacatcgt aacggaacat ggtgcaaaac tgtacaatga
     3421 cataatgcag gcagccctct ttaccggggg cgttttttat aatgtaatga tataattcat
     3481 gatccgaacc ggaaaaatgt tgagcagctt ttataagcca gcgatttttc tggtcacctg
     3541 gcatacttca ggatagctga cgcccttatc cggtccgtct tcccgcatcg gccgccgatt
     3601 ctgccatgtg atgaaaaaac cggaaggctt tttccgggga ttttttcatt gccggggcat
     3661 attaaatggt gccatggccg ccgcctgata acaactggct gactggcgtt cctggcacct
     3721 gaaaaatttt ttcaaacgtt ccggcatcca cgatcgtgtt agtaatgaag ggagggaagc
     3781 tgatttgcga ggttctttat tgcgtacgag tgaagagttg ctggaaatct ataaccgtca
     3841 tgtggatact gtctaccgcg tttgcttcat gttcatgaaa aacgtgcctg atacggagga
     3901 catggtccag aacacattca tcaggcttat gaaggacaag acggttttcc gggatgagga
     3961 acatgagaag gcctggctca tcaggacagc gtccaatcta tgcaaggacc acttcaggca
     4021 ttggtggtca aagacggtag gtatcgatca ggccggtgag acggcggtcg atcaggattt
     4081 caatatagac agcagcctcg aaaaagtttt ggcacttccc tccaaaataa ggactgccgt
     4141 gtatttgtac tactatgagg gctactccac ggtggagata gcgaagatac tgggaaagaa
     4201 cgagtcgaca gtcagggggt atctccatac cggcaggaag cttctgaaaa tggaaatgga
     4261 aggtggtctt gagtgaagaa agaagatctg aggaattctt tcgatttgat aaaaccggat
     4321 gaaaatgcga agaagaggat gctggacaat ataatgcgga gcgccggtaa aaaagacgga
     4381 agccgcagga tgtatttcgt gaaagccata cccgcttttg ctttgactgt cgtggtagcc
     4441 gctggcgtgc tgatgtacag cctgaagccg ggcaataacg gatcgccgcc cggacaggtt
     4501 acagatcttg ccgggggcat cggcagggaa gatgcggctg cccctctcct ggaccagttt
     4561 cgggtcgatg acaggcatta tatcctgctt acagatgaac tgaggaatga tttcggactg
     4621 ccgtccgaaa taaaagaagg cgacattggt gaaaagctga cggatatcac ggtcagcccg
     4681 gatgagagcc tgacaggcag agaagtgtac agatatgttc cggccggctg tgaagcggta
     4741 gtagccgtaa aaggggatga cggataccgg cttttcaggt ttttcacatt tgaaagctac
     4801 aatagcaacc aggatgagga tgccgtggaa taccttgaac tttacggcat caacagtgcg
     4861 gaagatatct caaaggtcct gttgatcggc cattcggagc agggtaagat gtctggcgtc
     4921 gatgatatcc gggaaataac tgacaggagt gaaatcgagc agttctatgg ttattattcc
     4981 gtgctgaaga acaacagcga caaatacttt gacaggctgt tcaactataa tggtacgggc
     5041 actggcaaca gcagcgtcgg ggtagatatc gccgaacggg aaaggatggc ggctgccgaa
     5101 ccggccgggc cgatcgagcc ggatgcagcc ggcttcggat cagacatgcc gatggagatc
     5161 gcgccggctg caccggatac acctgtttca gcgaatgtcc cggcccctgc agatgccccg
     5221 gtgccgcaga agccgtcggc aacagccgat ccatccgccg gaatcgtgac ggacatgggc
     5281 ggcgggacaa caggatcggt acagggcagc acaggttcga caggcgatgc cctggatctt
     5341 cccataacaa taaggatata caacaggaac ggtatttact tcgattcggt gtactacagg
     5401 aacataggct tcatcaacag gtacgaggta agtgatgagt ttgcagcctt tctaagcgcc
     5461 aggttctgaa aaagagcatc agatgacaca atggcccaat aacctgatag cccgataata
     5521 taaaaacgac actgtaactg agcggctaag ctgcctggct gctcggccgg ggaaacgatt
     5581 ggacagttgt tggtttgcat gaccgcctcc tggtattgca cagtaccggg agtttttttc
     5641 tttacagata tgtgtatatg ctgtgccagg cacctggtta ttatatataa cggctggctg
     5701 atatgttata atatttccag gtgccggccg cagggatcca tatccgacgc tgccgggcat
     5761 ggcgccggga aggaggagaa cgatgctgaa gtacatagtt cctgaataca agaggctgag
     5821 ggtcatcgtt gatacagatg ccgcatgcga ggcggatgat ccgtttgcga tagcgcatgc
     5881 gctcatgtgc cgcaagtttg acgtaaaagc gatattagcc gaacagttcg gcggcccgga
     5941 aacgactaaa aagagctatg acgagatcat gaccgtcctg gatgccatgg atatttcagt
     6001 cccggttttt atgggcgaag aaggcaagct ttctgatata ccgggcgatg acatatctcc
     6061 ggcttcgaag ttcctggccg aagaagcgat gagggatgac gacaggccgc tgttcgtgct
     6121 gtgccttgga gcggtcacga atgtcgcgtc tgcgataaga agggctcccg gtatcattcc
     6181 gaggatgact gtcgtatgga tcggcggaca gggttatgat tcatatacgc ctgatttccg
     6241 cgagttcaac tcgggaaacg atatcgatgc cgctaacctg gtggtttcaa gcggtgtcga
     6301 gttatggcag ataccgtccg atgtttatgg ctcgatgcgt atcagccttg cagagataca
     6361 gcacaggata tatccctgcg gaaagatagg gaagcatttg tttgaaaaca tggtggacta
     6421 taatatgtcg aagcatgcag gatggacggc cggcgaaagc tgggcgctgg gagacaatcc
     6481 tgcagtcggg gtggctatgg accagaactg cggccgcttt atttaccgcg aagcccctat
     6541 tttcaatgag gacaccactt acaccttcga agccggcaga ccgaaaatcc gagtgtacac
     6601 ttccatagat ccgaggttca ttcttgaaga ccttataagc aagctgcagc tgatttacgg
     6661 cgcagggcat gacaggcaga cataattatt tgaatcgatg cttttgctgc ttagtaaacg
     6721 gagtgcttac gaaacacgac atagtataca tgttgcaatt cacaagccat atatactatg
     6781 atacggaaac cacgttttat gcaaggagga cagcatatgc caggcaacgg attcgaacag
     6841 ccgtacaggc acgtagtctc aggaacggta accggcaggt atgaagtgcc cgattgtcct
     6901 gaatgggcgc acagtgccgt attctaccag atctatccgc agagcttcta tgattcgaac
     6961 ggtgacggca tcggcgatct gcagggtatc atacagaagc tcgattatgt aaagagcctt
     7021 ggagcagacg ccatctggat aaatcccttt ttcgaatcgc cgttcaacga cgcgggatat
     7081 gatatttcgg actattacaa ggtcgcgccg cggtacggta caaatgagga cgcccggatg
     7141 ctttttgaag aagcccataa aaggggcatt aaagtgcttt tcgatttcgt ggccagttat
     7201 acatccatcg aacacgagtg gttcagggaa tcctgcaggc aggagaagaa cagatactcc
     7261 aactggtaca tatggaccga cggcacctgg gctccgatgc ccgaggagca gaaagacagg
     7321 ttcatacagg gctatgcgag acgcgacggg cagttcatga ggaacttcta ctggagccag
     7381 ccggctctga attacggata tgagcatccg gatcccgcgt ttccatggca gcttccgacc
     7441 gaccatcctg atgtgcttgc catgagggaa gagatgaaaa acgtggtaag gtactggatg
     7501 gatcttggcg ccgacggatt cagggcggac atggcgggcg cgctggtcaa gtcgacacgg
     7561 tacaggggga acagccagtt ttacgccaat aaagcatccg ggacccagaa gttctggcag
     7621 gaagtgcggg aaatactgga ccgcgaatac ccgggttcct tcatggtagc ggaatggtcg
     7681 catcccatat ccgcgatcga gggcggattc catgcggact tctaccactg gttcagcggt
     7741 ttcaacgacc tgttccagaa agaaagctgg aggatcctca acgggctttc cgaggggcac
     7801 agcttttttg acgccgaagg aaaggggaac atcacggact tcctggccgg ctacatggaa
     7861 cagtatgaag ccgttaaggg caaaggcttt gtctgcatac cggtggggaa ccatgacatt
     7921 gccaggataa ataataaccg ttcaccaaaa gaacttgaaa tgatcttcgc attctcgata
     7981 actctgccgg gcatgccttt catctattac gggaacgaga tcggaatgag gcagctccac
     8041 ggcctgcccc atgtggaagg cgcatacaag ccaagggcgg gcgctcgcac gccgatgcag
     8101 tggaaaaagg gaccgaacgc cggattctct gcggcgcctg ccgaaaagct ttatcttcct
     8161 gttgatccgg ccgacgacta tccgaatgta gaggaacagg agaaaaatcc cgattccctg
     8221 ctcaacaggg tcagaaagct gatatcgctg agaaaacggt acaaggcgct gtctgctcac
     8281 gccgaattcg ttccgttgta tgctgtggaa aacgggtatc ccttcattta tgcccgggcc
     8341 gccgacgagg aagtgatact ggtggtgctc aacccgtccg gccaggaaag aaaagccgaa
     8401 ttcaccagca acctcgagat ctccggcagg aagctgatcg caggagaaga aatcaggatg
     8461 gactccgacg gcagcaaaat ctctgtagca gtacctccta taacctattc tgtttacata
     8521 atgaacgcaa ggcactggag ttaaggtatg atcactttat cgaacagaca gacaaggcaa
     8581 tttttactgc tcaggcatgg attgctgggc gaacacaggt ttgccggcaa acatggtgcg
     8641 cttgattttg tacggcaggc aggatgcatc cagttcgatc ctgtcgatgt ctgcgggaaa
     8701 aacgcggagc ttacgctgca gtccaggatc aagggattca cgaagaatac gctttatgaa
     8761 ctgctgtatg tcgacaggaa actggtggac tatcccgaca aaaacctttc cataatcccg
     8821 gcagaggact ggccgtattt tgagcgctac aggcaggcag ccatagcggg cggccggcgg
     8881 ttcaaagagc tcgccggact ggcagaccag gcaaaggctt atataaggga gcacggtccc
     8941 gtctgttccg acgacctgcc gataacaggc ggtatccgct ggcattcatc catccactgg
     9001 agcggcaact ggaccggcat tacaaaggct gcgaggtctg tgctggaaca tctttattcg
     9061 accggcgaac ttgtcatcca ccataagaat ggctccagga agttctatga tctcgccgaa
     9121 aggcacatcc cgcccgagat actgtatgct ccagatccgc tgccggatga attccagcat
     9181 aaaaaatggc gtatacttcg gcgcatcgga gcggtgggac tgttgtggga ccgcccgtcg
     9241 gatgcatggc tcaacatttg gggccttaat gcccgggaga tctacactcc cgccgacaag
     9301 cggaaatatg gccattatgt ccttccgctg ctgtatggtg agaactttgt cggccgcgtg
     9361 gaagcggccg ctgataaaaa gacctgcacc ctgatattga gaaacatctg gtatgaaggt
     9421 aatgtaaagc agactaagag actggctgac gcaatctgga aatgcataaa ccgttttgcg
     9481 gaattcaacg agtgctcggc tgtcaaatcg gaatgcgctt tgatgaaaga ctgaagcacg
     9541 gatatgaaag actgcatcaa tgaactgaaa ctgaagcagt ccatttttcc gatctctcta
     9601 attatgtatt gatgtaattt tctcgaatgc tgctcacatg cggaaattct gatattcttt
     9661 atctggcagg gtaatgtttt tcaaggtacg atttttctcc atcctgcaaa catgcagggc
     9721 gtatgcaacg taaaatgtat gtaccaaaaa gattgcagcg aatatgcgcc acagtccggg
     9781 tatgtactgt tacttcttta tttggtataa ttggaaaagt tgcgctgttt gtattcaatg
     9841 aatagtgggg agtcaggaaa tgtcgtttga tgagttattg gtgttcatat cttcatgtat
     9901 ctattcagga tacagcatga tcagatggta taacaagctg ccgtcgtatg ggcctgtcaa
     9961 aagggataag gccgtaaagc ttgtgctggg tctgctgccc gtgatctcgt tttttatcat
    10021 tgtatatacg ctgcgggtac ttgcatcgtt cgatgtcgtg aatgatttcg tttacatact
    10081 gttttacatt gcgctgggat acgcatgggt tttccttgga atgcggtggg tcttccgctt
    10141 tttcgatatc tcgtggatcg acgatgtgct gcttctgaac aacaaggcgg ccctgatcac
    10201 tgtcgccggg gcattcctcg gactggtgac gatatattcg ggagccaaca taggagacgg
    10261 gcccggatgg tggtgcgtca tattcgcagg cgggctgggc ctggtctcgt ggttcctcct
    10321 tggcaggctt gtcaataagt tcgcacaggt ttttgagcgg ataacggtgg acagggacat
    10381 ctattgcggg atccgcaccg gcgcttatct gctggccggc gggatcatcc tggctcgtgc
    10441 gtccgctggc gactggactt cattttccaa aacgatagtc gagttcatgg tcgg
//
LOCUS       DAOSCC010000072        10403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4232_length_10403_cov_4.150754, whole genome shotgun sequence.
ACCESSION   DAOSCC010000072 DAOSCC010000000
VERSION     DAOSCC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4232_length_10403_cov_4.150754"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..814)
                     /gene="trmD"
                     /locus_tag="PLG72_05360"
     CDS             complement(77..814)
                     /gene="trmD"
                     /locus_tag="PLG72_05360"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOJ80260.1"
                     /translation="MRFDVLTIFPEIFDVVLGSSIIGRAREKGLITVKAWNIRDYTND
                     KHKKTDDYPYGGGSGLVMMAQPIYDAYEAVVRDLDYKPHFVYMSPQGKLLDQKLVEQL
                     AAHKHIILLCGHYEGVDERVLEKLVDEEISIGDYVLTGGELPAMVLIDAVSRAIPGVL
                     SNEESYADESHKNGLLEYPQYTRPYEFMGMKVPDILLSGHHANIEKWRRLQSLKRTRD
                     KRPDLFEKFVLSEEDKKLLDADTDSRF"
     gene            complement(804..1322)
                     /gene="rimM"
                     /locus_tag="PLG72_05365"
     CDS             complement(804..1322)
                     /gene="rimM"
                     /locus_tag="PLG72_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516314.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOJ80261.1"
                     /translation="MYDYLQVGKIVNTHGVKGEVKLLPLTDDISRFDDLERVFVESDG
                     TMTRYDIQSVKYFKGMVIIKLAGIDDPETAAALKGCFALVDRENAVKLPEDTYFICDI
                     IGCSVYDENGAYLGQVREVLQTGSNDVYAVRDETGREVLLPALKSVVRKVQPDLRRID
                     VVIPKGLLDDEV"
     gene            complement(1446..1673)
                     /locus_tag="PLG72_05370"
     CDS             complement(1446..1673)
                     /locus_tag="PLG72_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ80262.1"
                     /translation="MKELLETIARSLVDHPEEVRVNEIENDSTVILELSVAREDMGKV
                     IGKQGRIAKAIRTVIKAAAIKENVKAVVEIV"
     gene            complement(1734..1979)
                     /gene="rpsP"
                     /locus_tag="PLG72_05375"
     CDS             complement(1734..1979)
                     /gene="rpsP"
                     /locus_tag="PLG72_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOJ80263.1"
                     /translation="MAVKIRLKRIGAKKNPHYRIVVADSRYPRDGRFIEEIGTYDPQT
                     EPSTVNIDTEKAAQWIRNGAQPTDTVRSLLKKAGVIQ"
     gene            complement(2032..3375)
                     /gene="ffh"
                     /locus_tag="PLG72_05380"
     CDS             complement(2032..3375)
                     /gene="ffh"
                     /locus_tag="PLG72_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ80264.1"
                     /translation="MAIFEGLSAKLQETIGRFRGKGRVTEKDVKEMMREIKLALLEAD
                     VNYKVVKNFINSVSERAAGQEVLESLTPGQQVIKIVHEELIDLLGKVPSKPTFASKPP
                     TIYMMVGLQGSGKTTTSGKLANLLRKQGKNPLLVACDVYRPAAVKQLQVVGGQLGVPV
                     FAIEGSTDPVHIAKKAVEHAVSRMYDLVIIDTAGRLHIDEELMDELENIKNAVHPHEI
                     LLVVDSMTGQDAVNVAESFNEKLGIDGIVLTKLDGDTRGGAALSVKAVTGKPVKFATT
                     GEKLNDIEPFYPDRMASRILGMGDVLSLIEKAQEAFDEKKAIEMEKKLRTQQFTLDDF
                     LDQMQQIKKMGPLNQVLGMLPGMNNKALSNIEVDEKKLARTEAIIKSMTPQERRNPDI
                     INGSRRKRIAAGSGTSVQEVNALLKQFEEMKKMMKMVSNIGKKGGKGLGRFRLPF"
     gene            complement(3379..3732)
                     /locus_tag="PLG72_05385"
     CDS             complement(3379..3732)
                     /locus_tag="PLG72_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOJ80265.1"
                     /translation="MEELFKVSLLLDFYGQMLTKRQYDILDLHYNSDCSLAEIAEELG
                     ISRQGVHDNIKRGKAALFDLERKMGLVARFNAQKEKASEALKLLEDIETAVTDSRDKE
                     KLSKAKLLIRSMMED"
     gene            complement(3843..8354)
                     /locus_tag="PLG72_05390"
     CDS             complement(3843..8354)
                     /locus_tag="PLG72_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ80266.1"
                     /translation="MSSRKTPENECMQGFSPLIRDWFLENIGAPSEPQRRGWPKIASG
                     RNVLICSPTGSGKTLAAFLKCLDWIISEKTSSGKKQDERNRGIRIIYISPLKALNNDI
                     YRNLELPLSGLKKKASEQGMEMPEIKISVRTGDTPQKERTRMLKDPPDILITTPESLF
                     IMLTSDSYRKLFSTTEYIIVDEIHSICPTKRGVHLSLSIERVERIAGKPLKRIGLTAT
                     INPLEEAARFLSGGRNVTVINCERKRPLELSLSLPVKDLKVLPENTVWPSIYAELLAL
                     VRAHRSTLIFVNNRRVAEMVAAGMNSLAGEPFVRTHHGSVSREIRHELERQLKEGEIP
                     CLVATSSLELGIDIGSIDLVVQVSSPGTASQVLQRIGRSGHRIGAVSKGVIIPKTRGD
                     LLDSAFISYQAKRYDIENFRVPQNCLDILAQQIVSIACEGEQDKDGIYEMMRKAYPYR
                     DLPRKQFEDVIIMLSDPSPEDAPGSVKPRIMYDRGSGMVRGTPLGRRMCLMDGGTIPD
                     KGNYNVYLMDTGTKVGELQEEFVFESRIGDRFFLGSSVWKIEKIEKDRVLVSHSNASG
                     AKIPFWIGDKTLRSYETGKKLGQFLQRLEKEYDTEGFFSFMSEECGLDRTAAENLKTY
                     IEDQLAATRHLPGADRIVCEHFSDETGDRRIVIHTPFGSRIHAPLAVILSSKLSRLLN
                     CRMEYIYNDNGILFHIFGYTGKLSNIFSLLDKNTMEDEIFELLPKDYMFNINLRYNLI
                     RSLLVEMKTYGKRTPLWIQRLRCAETAEHILGEPDHPAVVETYRECMNDIFDIRGLYD
                     LVDKISAGMIKVTDVYTEKPSPFTSELLFNFWQIYQYSYELPVAERRNQLLVNDRDFI
                     QLAAGQNAEYELLDPRAIKTVEKEMEKYKYGRTIKNADDLWYYLYSYGELRAEPYSVT
                     AFKGSAAEDLDVYLEQLESQRRIIRINIGPGNGIYWVAAEDFPLYFIAAGRDPENETV
                     KVGKPGGEKEVKAVNLLSSYVFDLAPGVRDAAVRLIRRHVMFSGPFRISDLTQKYGMD
                     AETADDALKALVSGGEVLKIKESDDPDECIYCHKKVYERIKQRTIKFARKDIKPKAPE
                     IYCSYLLNKHLVTDRVLSPDEKLPEVLKTLNGQYFPASWWEDFILPSRINGYNPKMLD
                     YLCATGQVRWRGRLSKNTKETAFFMTGEEDMRQDRFAEPDDLIHDPGSYEKPNDFTMD
                     ETEAGILEVLDKEGAVFLNDLSKRLKLPPSDLLAKMERLVWCGIITNDSFSVIRYYID
                     NEKKNSPWTKYNTYPSMGRWSKTSGYGDNEAVGSIAGYVNILLDRYGIVSKEIVNKEK
                     YGFNWTETYTWLKNNELASGIKRGYYISGLSGIQFMRDRDIELIRMMDTPSDTQEYVA
                     LCSCDPANPYRDILTGSVGSRVAARQSNAVVFCNGKPVLYIREYGHTIQQATDDTGVL
                     KDAASCFVETYRNRMLWTGKKNIFTEYWSDLSGNECFRIEDSPIYDALLDLGYERGYS
                     GITLWRKAL"
     gene            complement(8450..9358)
                     /gene="ftsY"
                     /locus_tag="PLG72_05395"
     CDS             complement(8450..9358)
                     /gene="ftsY"
                     /locus_tag="PLG72_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOJ80267.1"
                     /translation="MGFFSKLKEGLAKTRKGITEKIDQVLVSFGKVDEELFEELEEVL
                     VSADLGVETAMKIMDDTREKVKERKITDPLKIKELIKETLVEILGEETQKLDIEPSPA
                     VIMVIGVNGVGKTTSIGKIAYALKNQGKKVMLAAADTFRAAAIDQLEIWADRVGVDII
                     RQQEGSDPAAVVYDAIQAAKARKADVLICDTAGRLHTKKNLMEELRKVSRIIDREMPG
                     ARRENLLVLDATTGQNAVSQARTFSEVTDISGIVLTKLDGTAKGGIVIAIRSELGLPV
                     KLIGVGEQMDDLQPFNAKEFVEALFS"
     gene            complement(9360..>10403)
                     /locus_tag="PLG72_05400"
     CDS             complement(9360..>10403)
                     /locus_tag="PLG72_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ80268.1"
                     /translation="RTEKENMERSIGKKESEQDRFRNEIKKLEEQIEGLNARIKKHLE
                     EKSGKTFEIDRLTEERKILEEDIYDIVNKINDINKNMLLLKEEYSRTEVKKTRLEAEM
                     EAMQDKIWNEYELTYSTALELKKDIGSHSAAQKRISELRNMIKDLGPINVGSIDEYIK
                     TKERYEFMNSQRNDMEQAREKLLRVISEMTSIMKKQFLEQFELINKNFNDVFRELFNG
                     GRAGLTLVDRENVLESGIEIEAQPPGKKLQNMMLLSGGERAFTAIALLFAILRLKPTP
                     FCVLDEIEAALDEANVYRFVEYLKNYSCNTQFIVVTHRKGTMEGSDELYGVTMEEHGV
                     SKVVSMKMGEKVG"
BASE COUNT         2183 a         2848 c         2200 g         3172 t
ORIGIN      
        1 gcttaaaaca gggttctgta agattttgta aggcaattaa acttgcaatt taaagctgct
       61 gccatgcagg tcaaattcaa aacctgctgt cagtatctgc atccagcagc tttttatcct
      121 cttcggacag tacgaatttt tcgaagagat ccggtctctt gtcccttgtc cttttcagcg
      181 actgcagcct tctccatttt tcgatattag catgatgtcc tgacagcaga atgtcgggaa
      241 ccttcatgcc catgaactca tatggcctgg tatactgggg atattccagc aagccgttct
      301 tatgagattc gtcggcatat gattcttcat tggacagtac tcccgggatc gctctgctta
      361 ctgcatcgat cagcaccatt gcaggaagtt ctcccccggt aagcacatag tcgccgatcg
      421 atatttcttc gtcgaccagt ttttccagca ccctttcatc cacaccctcg taatggccgc
      481 acaacagtat tatgtgcttg tgtgccgcaa gctgctccac gagtttctga tcgagcagtt
      541 tgccctgggg gctcatgtat acgaaatggg gcttgtagtc caggtccctt actactgctt
      601 cgtatgcgtc atatataggc tgagccatca ttacgagccc gctgccgccg ccataaggat
      661 agtcgtcggt tttcttgtgc ttgtcatttg tataatcccg gatattccat gctttgaccg
      721 ttataaggcc tttttcccgg gcacgaccta ttatactgct tcccagcact acatcaaata
      781 tctccgggaa tatggtcagt acgtcaaacc tcatcatcca acaatccttt cgggataacg
      841 acatctatcc ttctcaggtc aggctgcact ttccttacga cacttttcag agcaggcagg
      901 agcacttccc tgcccgtctc gtccctgacc gcatacacat cattgctccc ggtctgcagg
      961 acttcgcgga cctgcccaag atacgcgccg ttctcatcgt atacgctgca gcctattata
     1021 tcacagatga agtaggtatc ctccggcagt tttacggcat tttccctgtc cacaagagcg
     1081 aagcatccct tcagggccgc agcggtctca gggtcatcga tgcctgccag cttgattatg
     1141 accatccctt tgaagtattt cacggactgg atgtcatacc tggtcattgt tccatcgctt
     1201 tcgacaaaga cccgctccag atcatcaaac cgggatatat cgtcggtaag aggcaacagt
     1261 ttaacttcgc cctttactcc gtgagtgttc acgattttgc ctacctgcag ataatcgtac
     1321 atttatgcct ccgttataaa aaatgtattc cggaagtacc ataaacagat ttccggacca
     1381 gccagcgtgt tcctttaaat acatggagga cagcaaataa aaaggtgttg tgaaacacct
     1441 ttttattata ctatctccac cacagccttt acattttcct tgatggctgc ggcttttata
     1501 actgtacgaa tagccttggc tatccgtccc tgttttccta tcaccttgcc catatcttcc
     1561 ctggcgacac tgagttcgag gattacggta ctgtcatttt ctatttcgtt gacacgtacc
     1621 tcttcgggat gatcgaccaa agacctggcg atagtttcga ggagttcttt catttcattt
     1681 ccctcctgct tctcaaatac ctcccgcatt acagcgtcca catcccggaa cagttactgg
     1741 atgactcctg ccttcttcag cagagatctc accgtatcag tgggctgtgc tccattcctg
     1801 atccattgtg ccgctttctc agtgtcgatg ttgactgtgg aaggctctgt ctgcggatcg
     1861 tatgtgccta tttcctcgat gaacctgccg tcgcgcggat acctggaatc agcaacgact
     1921 atcctgtagt gcggattctt ctttgcaccg atcctcttca acctgatttt tactgccatt
     1981 tctgtcacct cctctcaagt tgcgtggatc atatccaaac cctgataata tctaaaaagg
     2041 caacctgaac ctgcctaatc ctttgccgcc ttttttgccg atattgctca ccatcttcat
     2101 cattttcttc atctcttcga actgtttgag cagggcgttc acttcctgga ccgatgtccc
     2161 gctgccggcg gctatcctct ttcttctgct tccgttgatt atatccggat ttcttctttc
     2221 ctgcggagtc attgacttaa tgattgcttc agtacgcgcc agcttttttt cgtcaacttc
     2281 tatattgctg agcgccttgt tgttcatccc cggcagcatg ccaagcacct ggtttagagg
     2341 acccattttc tttatttgct gcatctggtc gagaaaatcg tccagtgtga actgctgcgt
     2401 ccgcagtttt ttttccatct cgatggcttt cttttcatcg aatgcttcct gcgccttttc
     2461 gatcaggctc agcacatcgc ccatcccaag tatcctggaa gccatcctgt cgggataaaa
     2521 cggctctata tcgttcagtt tttcacctgt agtggcgaac ttgacgggtt tcccggtgac
     2581 cgccttaact gaaagcgccg ctccgcccct ggtatcgccg tcaagctttg tcagcacgat
     2641 accgtcgata cccagctttt cgttgaagct ttcggcaacg ttaacagcat cctgcccagt
     2701 catcgagtcg accaccagca gtatttcgtg gggatggact gcgtttttga tattttccag
     2761 ttcatccatc agttcttcgt ctatatggag tcgaccggcg gtatcgatga taacgagatc
     2821 atacatcctg ctgactgcat gctcgactgc cttctttgct atatgcaccg gatctgtgct
     2881 gccttcgatg gcgaatacgg gcactccgag ctggcctccg acgacctgca gctgcttcac
     2941 ggccgctggc cggtatacgt cgcatgcgac aagcaaaggg tttttgccct gcttcctcag
     3001 aaggttcgca agtttgcctg aagtcgtggt tttaccggaa ccctggagtc cgaccatcat
     3061 atatatcgtg ggcggtttgc ttgcaaatgt gggctttgaa ggcactttgc caagcaggtc
     3121 tatcagttcc tcgtgcacta tttttatgac ctgctgcccc ggtgtcaggc tctccagcac
     3181 ttcctgcccg gctgcgcgct ctgaaacact gttgatgaag tttttcacca ctttatagtt
     3241 aacgtcggct tcgagcagag ccagttttat ttcccgcatc atttccttta cgtctttctc
     3301 ggttaccctg cccttgccgc ggaacctccc aatggtctcc tgcagtttgg cggacaaacc
     3361 ttcgaatatt gccattcgtc aatcctccat catacttctg atcaatagct ttgctttgga
     3421 aagcttttct ttgtcccggc tgtccgtgac ggctgtctca atgtcctcca gaagtttcag
     3481 tgcctcggac gccttttcct tctgggcgtt gaaccttgcg accagtccca ttttcctctc
     3541 caggtcaaat agtgcggcct tgccgcgctt tatattgtca tgcactccct gtctgcttat
     3601 gcccagctct tctgcgattt cagccagcga acagtcgctg ttgtaatgca gatcaaggat
     3661 atcatattgc cgctttgtca gcatctgacc gtaaaagtca agcaaaaggc ttaccttgaa
     3721 taactcttcc ataatcgtaa aaaaggtgta aagtattttt actttacacc taattttaag
     3781 ctttggcctc tgtcatgtca agggtaattt tgatcacatg cgcaaaaaat caaaataaag
     3841 ctctatagtg ctttcctcca tagcgttatc ccgctgtaac ccctttcata cccgagatcc
     3901 agcaaagcgt cgtatatcgg agaatcttcg atcctgaagc attcattgcc cgacaggtcc
     3961 gaccagtatt ccgtgaatat attctttttt ccggtccaga gcatcctgtt cctgtaagtt
     4021 tcaacaaagc aggacgccgc atctttaagt acccctgtat catctgtcgc ctgctgtatc
     4081 gtgtgcccgt attccctgat atacagcact ggctttccat tgcagaaaac cacggcattg
     4141 ctctgccttg ccgcaaccct ggatccaact gagcccgtga gtatgtccct gtaaggattt
     4201 gcaggatcgc agctgcacag ggcgacgtat tcctgtgtgt ctgacggagt atccatcatc
     4261 cttatgagtt ctatatccct gtccctcatg aactggatcc cggaaagacc ggaaatgtaa
     4321 tatcctctct tgatcccgct ggcaagctcg ttgtttttca gccacgtata ggtttctgtc
     4381 cagttgaagc cgtatttttc cttattgact atttcttttg acactatacc gtaacgatca
     4441 agcaaaatat taacatagcc ggctatgctt ccgacggctt cattatcgcc gtaaccgctt
     4501 gtcttggacc atcttcccat gcttggatat gtattgtatt ttgtccaggg cgaatttttc
     4561 ttctcattgt ctatataata cctgattact gaaaaagaat cgtttgtgat gatgccgcac
     4621 catacaagac gctccatttt tgcaagcaga tccgaaggcg gcagtttcag cctttttgac
     4681 agatcattaa ggaaaacggc gccttctttg tcaagcactt cgagtatccc tgcctcggtt
     4741 tcgtccattg taaagtcatt cggcttttcg tatgaccccg ggtcatgaat cagatcgtcc
     4801 ggttccgcaa aacgatcctg ccgcatatct tcttcgccgg tcatgaagaa tgccgtttct
     4861 ttcgtgtttt tgctcagcct gcctctccat ctcacctgcc ctgtggcgca caggtagtcc
     4921 agcatcttcg ggttatatcc gtttatcctg gaaggaagta tgaaatcctc ccaccatgag
     4981 gccggaaagt actggccatt gagcgtcttc aggacctcgg gaagcttttc gtccggtgaa
     5041 agcactctgt cggttacaag atgtttgttc agcaggtatg aacaatatat ttcaggggct
     5101 ttaggcttta tatcctttct tgcaaacttg attgtccttt gctttatcct ctcatagact
     5161 tttttgtggc aatatatgca ctcatccgga tcatccgatt cttttatttt cagtacttca
     5221 ccgcctgaaa ccagggcctt tagcgcatcg tcggccgtct cggcatccat tccgtacttt
     5281 tgggtcaggt cgcttatcct gaaaggacct gaaaacataa catgacgtct tatcaatctt
     5341 acagcagcat ctctaacgcc tggagcaaga tcaaatacgt agctgctgag caggtttact
     5401 gctttaacct ccttttcccc gcccggcttg ccgactttaa ccgtctcgtt ttccggatct
     5461 ctgccggccg cgatgaaata cagggggaaa tcctctgctg ctacccagta tataccgttc
     5521 cccggaccga tgtttatacg tatgatcctc ctctggcttt ccagttgttc gagatataca
     5581 tccagatctt cagcggcaga acctttgaaa gcggtaaccg aatagggctc tgccctgagt
     5641 tctccataag agtaaaggta ataccagaga tcatctgcat ttttgatcgt tctgccatac
     5701 ttatattttt ccatttcttt ttccacagtc tttatcgctc tggggtcaag cagttcgtat
     5761 tcggcattct gtcccgccgc aagctgtatg aagtcccggt cgtttacaag gagttggttc
     5821 ctgcgttccg cgaccggcaa ttcgtacgaa tactgataga tctgccagaa attgaacaga
     5881 agctcggatg tgaaaggcga aggcttttca gtatatacat ccgtcacctt tatcattccg
     5941 gcgcttattt tatcaacgag atcatataaa cccctgatgt caaaaatatc gttcatgcac
     6001 tccctgtatg tctcgacaac ggcaggatgg tcgggttctc cgaggatatg ttccgctgtt
     6061 tccgcacatc tcagtcgctg tatccacagc ggggtccttt tcccgtatgt tttcatttca
     6121 acaagcaacg accggataag attgtaacga aggtttatgt tgaacatgta atctttgggc
     6181 agaagttcga atatttcatc ttccattgtg ttcttatcca gcagcgaaaa aatattcgac
     6241 agtttgccgg tatatccgaa tatatgaaaa agtatcccgt tatcgttgta tatatactcc
     6301 atcctgcagt tcagcagcct tgaaagcttt gaactcagaa taacggcaag aggcgcgtgg
     6361 atcctgcttc caaaaggagt gtgtatgaca atgcgcctgt cgccggtctc gtcgctgaaa
     6421 tgctcgcata ctatcctgtc ggcgccaggc aggtggcgcg tggctgcaag ctgatcttct
     6481 atatatgttt tgaggttttc ggcagcggtc ctgtcaaggc cgcattcttc ggacatgaag
     6541 ctgaaaaatc cttccgtatc gtattccttc tcgagccttt gcaggaattg acccagcttt
     6601 ttgcctgtct cataactccg cagcgttttg tctcctatcc agaaaggtat ttttgccccg
     6661 gaggcattgg aatggctgac cagcaccctg tccttttcga tcttttctat cttccagacg
     6721 gaactgccaa ggaaaaacct gtcgcctatc ctgctttcaa atacgaactc ttcctgcagt
     6781 tcccctacct tggtaccggt atccatgaga tatacattgt agttgccttt atcggggatc
     6841 gtaccgccat ccatcaggca catccttctg ccgagaggcg ttccccttac cattcccgaa
     6901 cccctgtcat acatgatcct tggcttcact gaacccggcg catcttccgg cgacggatcc
     6961 gacagcatga tgatgacatc ttcgaactgc ttccgcggca gatccctgta aggatatgct
     7021 tttcgcatca tttcatatat cccgtctttg tcctgctctc cttcgcaggc gatggagact
     7081 atctgctgtg ccagtatatc aaggcagttc tgaggcaccc tgaagttttc tatatcatat
     7141 cgtttcgcct ggtatgagat aaaagcggaa tccaacaggt cgcctctcgt tttgggtatg
     7201 atgactccct tgctgactgc gccgatcctg tgtcctgacc ttcctatcct ctggagtacc
     7261 tgggacgccg taccgggcga acttacctgt actacaaggt cgatactgcc aatatcgatg
     7321 ccaagctcca atgaggacgt ggccacaagg cacgggatct cgccttcctt gagctgcctt
     7381 tcaagttcat gcctgatttc ccttgatacg cttccatgat gggtcctgac gaaaggttcg
     7441 cctgccaggc tgttcatgcc tgctgcgacc atttcggcca ctctcctgtt gtttacgaag
     7501 atcaaagtcg atctgtgcgc ccttaccagt gccaaaagct cagcataaat ggaaggccat
     7561 accgtatttt cgggaagcac cttcagatcc ttgacaggca ggctcaatga aagctcaaga
     7621 ggcctttttc tttcacagtt aataacagtc acattcctgc cgcctgacag gaacctggca
     7681 gcttcctcca gcggattgat ggtagcagta aggccaatgc gtttcagcgg ttttcccgcg
     7741 atcctttcca ccctctcgat ggacagggaa aggtggactc ccctcttggt cgggcatatg
     7801 gaatggatct cgtccacaat gatatattcc gtcgtactga acagcttcct gtaggaatca
     7861 gaggtcagca tgatgaaaag cgattcgggc gtcgtgataa gtatgtccgg aggatccttg
     7921 agcatccttg tcctttcctt ctgaggcgta tctcccgttc ttaccgagat ttttatttcc
     7981 ggcatttcca tgccctgttc ggatgctttc ttcttcagcc cggaaagagg aagctcaaga
     8041 ttcctgtata tatcgttatt caaagctttc aaaggtgaaa tgtatatgat cctgatgccc
     8101 ctgttgcgct catcctgttt tttcccggat gaagtctttt cactgattat ccagtcaagg
     8161 catttcagaa aagcagcaag ggttttgccc gaaccggtcg gagagcatat cagcacattc
     8221 ctgccggacg caattttagg ccagccgcgc ctctgtggtt cgcttggcgc accgatattt
     8281 tcaaggaacc agtccctgat cagcggactg aagccttgca tgcattcatt ttcgggtgtt
     8341 ttcctgctgc tcatatcgac tccctgagaa taaataatta cattcaggcc atgtcatcgg
     8401 cacagccgtg cggatgtaac acaaatctat ttacattgcc atgtgaatgt catgaaaaca
     8461 gggcttctac aaattccttg gcattgaaag gctgcaggtc gtccatctgt tcgccgactc
     8521 cgatcagttt gacaggcagc ccaagctcgg accttatggc aatcacgatg ccgcccttcg
     8581 cagtaccgtc aagctttgtc agcacgatac ctgatatatc ggtgacttca ctgaaggtcc
     8641 tcgcctgtga aacggcgttc tgtccggtag tcgcgtccag gacaagcagg ttttcccgcc
     8701 ttgctcccgg catttccctg tcgattatcc ttgaaacctt ccgcagttct tccatcaggt
     8761 ttttctttgt gtgtaggcgt cctgcagtat cacagatcag tacgtcagct tttcttgctt
     8821 ttgccgcctg tattgcgtcg taaacaacag ccgcgggatc agatccttcc tgctgcctga
     8881 ttatgtcgac cccgaccctg tctgcccata tttcgagctg gtctatcgca gctgccctga
     8941 atgtatccgc ggctgcaagc atgacctttt tgccctggtt tttcagggcg taggctatct
     9001 tgcctatgga tgtcgttttc ccgacgccgt tcaccccgat caccataata accgcaggag
     9061 acggctctat atcaagcttt tgcgtttcct cgcccagtat ctctacaagg gtctccttga
     9121 tcagttcctt tattttcagc ggatccgtga tctttctttc ttttaccttt tccctcgtat
     9181 catccattat cttcatggct gtttcgacac cgaggtcggc agacacaagc acttcctcca
     9241 gttcttcgaa cagctcttca tccactttcc cgaaagaaac aaggacctgg tctattttct
     9301 cggtaatgcc ctttcttgtt tttgcaagcc cttccttcag tttgctgaag aatcccattc
     9361 tatcccacct tttcacccat tttcattgaa acgacttttg atacgccgtg ttcttccatc
     9421 gttactccgt aaagctcgtc ggagccttcc attgttcctt tcctgtgcgt gaccactatg
     9481 aactgcgtgt tgcaggagta gttcttcagg tattcgacga aacggtatac attagcctcg
     9541 tcaagggccg cctctatttc gtcgagaacg cagaacggcg ttggtttgag cctcaatatg
     9601 gcaaacagga gcgcaatggc ggtaaacgcg cgttcccctc cggacagcag catcatgttc
     9661 tgcagcttct tgcctggcgg ctgcgcttct atctcgatac cgctttccag cacgttctcc
     9721 ctgtcaacca gcgtcaggcc ggcccttccg ccgttgaaaa gctccctgaa aacatcgttg
     9781 aagttcttgt ttatcagttc gaactgttcc aaaaactgct ttttcattat cgaagtcatt
     9841 tcggatatga cgcgcagcaa tttctcccgc gcctgttcca tgtcgttcct ctggctgttc
     9901 atgaactcgt agcgttcttt tgtctttata tattcgtcga tcgaacctac gtttatcggc
     9961 ccaagatctt ttatcatgtt cctgagttcg ctgatccttt tttgcgcggc cgaatggctg
    10021 ccgatatctt ttttcagttc aagggccgtc gagtatgtca gttcgtattc gttccatatc
    10081 ttgtcctgca tcgcttccat ttctgcctca agcctggtct ttttgacttc tgtccgcgag
    10141 tattcctcct tcagcagaag catatttttg tttatatcat ttattttgtt cactatatcg
    10201 taaatatctt cctcaagtat ctttctttcc tccgtcagcc tgtcgatctc gaatgtcttg
    10261 cccgattttt cctcgaggtg tttctttatc ctagcattca gaccctctat ctgctcttcg
    10321 agctttttga tctcgttcct gaacctgtcc tgttcgcttt cttttttgcc gatgcttctt
    10381 tccatgttct ccttctcggt acg
//
LOCUS       DAOSCC010000073        10384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4244_length_10384_cov_4.401201, whole genome shotgun sequence.
ACCESSION   DAOSCC010000073 DAOSCC010000000
VERSION     DAOSCC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4244_length_10384_cov_4.401201"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLG72_05405"
     CDS             complement(<1..204)
                     /locus_tag="PLG72_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80269.1"
                     /translation="MLCDSCGFKNSINAKKCINCGAELKSGISGEEISRMIDRLSDRS
                     DALTASECARILKWIFFSLAAAEL"
     gene            complement(210..851)
                     /locus_tag="PLG72_05410"
     CDS             complement(210..851)
                     /locus_tag="PLG72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOJ80270.1"
                     /translation="MWEKLELPWKAAFSQGWESFRNGSIPIGAALADENGNVISVGRN
                     RLYEPGSLNPRIAHAETECLQKLDTSKYPNLGQYTLYTCMEPCPMCFGTIVMSNIRKV
                     RVAAKDGYCGAVYLAETDPYIKSKNMQIEFGPGNLQGVQIVLQAYFELRAWNGETNKV
                     VDVFSQYCPEAVQTAKNFYKERYLDHWARTNKEFGEVLESIVSVLHGASISER"
     gene            complement(1081..1614)
                     /locus_tag="PLG72_05415"
     CDS             complement(1081..1614)
                     /locus_tag="PLG72_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOJ80271.1"
                     /translation="MRTIVIEPYNPEWPNEFAKIRDFLWPHIKDIAIDIVHIGSTSVP
                     GLAAKPIIDFDIVIESYDDFPRIAEQLKKLGYEHDGDGDVPTRERFKGGPREGFMAYN
                     MYVCPKDSMVLLAQILFRDYLREHKEARDEYAALKRSLAEKYRHDIDSYVDGKHEFII
                     NIANTALELRKDSLPYK"
     gene            complement(1616..2044)
                     /locus_tag="PLG72_05420"
     CDS             complement(1616..2044)
                     /locus_tag="PLG72_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80272.1"
                     /translation="MINMGTAGKSRENKNGFCSMQFVVKTVRKSIAAVLPLLVLFCVA
                     LLSYYAAGDRDYRAFARALAKNGGVRAVYAVKSTDDENFTASQFEEIREQLDKSLADK
                     GYESFYVSLDGSKYIVVEIAWTEGLDLDRRVVYADLNGGI"
     gene            complement(2010..2396)
                     /locus_tag="PLG72_05425"
     CDS             complement(2010..2396)
                     /locus_tag="PLG72_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOJ80273.1"
                     /translation="MGFWLFMTLMDMLIPVLMIGFGRSFMRKPPEKINPWYGYRTRMS
                     VKNKDTWDFAHRYAGKIWFVSGWIILLLTVPAVLMMLGQDERTIGTAGAVICVIQCVP
                     MAAATIPTEIALRRRYDKHGNRREVP"
     gene            complement(2441..2638)
                     /locus_tag="PLG72_05430"
     CDS             complement(2441..2638)
                     /locus_tag="PLG72_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80274.1"
                     /translation="MKSYNDILEWYRGTGLRPYLNVLDDEMQADFENDIMERLRKSYP
                     VQKNGEIIFRFPRFFFVAYFI"
     gene            complement(2638..3006)
                     /locus_tag="PLG72_05435"
     CDS             complement(2638..3006)
                     /locus_tag="PLG72_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80275.1"
                     /translation="MRALILDMYGVIMKDPECGFMPFVNRAFPDLSCEEIYKYWDKAD
                     LGEMSSLDFLRKLGFKGDLSKIEKEFLDTAEIDESFLTSGKGFSLKPGFIIHLLLVNI
                     LIYCLNSQITLPFGKPYTIL"
     gene            complement(3113..3688)
                     /locus_tag="PLG72_05440"
     CDS             complement(3113..3688)
                     /locus_tag="PLG72_05440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ80276.1"
                     /translation="MTHIFFVRHAQPDESWKDDRTRPLTETGLADRKRVTELLAKMHI
                     DCFYSSPYKRAMDTISECAGILNKEIYLDERFRERQPGEIGNVDFMDLLHKRWNDFNF
                     CEPDGECLASVQKRNIEALNEILAAHKDENIVIGTHGTALSTIMNYYDPAFNCDSFKR
                     IWFWMPYIVRLDFSGTGYIGREELLAIERGY"
     gene            complement(3892..4419)
                     /locus_tag="PLG72_05445"
     CDS             complement(3892..4419)
                     /locus_tag="PLG72_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ80277.1"
                     /translation="MARFYLVRHGEFTYKPVEERNFIGHGLELAPLTEDGIRQAVECS
                     KDKRLAGCDLIITSPYTRALQTAAILSKNLGIDIRVEIDLREHELDLSYQVKSHEDLK
                     RIASEEQKNNGLLPENGSFRWELRTDVKTRALNVLARYTTYKKVIVVTHGMVIHCLTG
                     AVGIPHCSVHEFDMD"
     gene            complement(4462..4629)
                     /locus_tag="PLG72_05450"
     CDS             complement(4462..4629)
                     /locus_tag="PLG72_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80278.1"
                     /translation="MYALACQVKPDITPEELWEKAFETSSALCGDDGSGDAAGRVVDP
                     AKLIESVEGMD"
     gene            complement(5158..5730)
                     /locus_tag="PLG72_05455"
     CDS             complement(5158..5730)
                     /locus_tag="PLG72_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOJ80279.1"
                     /translation="MGMIELFLVAVGLSMDAFAVAVCKGLATMELKFRHAAVTGLFFG
                     GFQAFMPLVGYLLGAQFSDYITAVDHWIVFALLSLIGLNMIRESRHDSCDNPHGSNTG
                     NSYDLKNMLMFSVATSIDALAVGVSFAFLKVEIIRAISIIGVTTFILSAIGVKVGSIF
                     GAKFKSRAETAGGIILIAMGVKILIEHLGA"
     gene            complement(5954..6670)
                     /locus_tag="PLG72_05460"
     CDS             complement(5954..6670)
                     /locus_tag="PLG72_05460"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HOJ80280.1"
                     /translation="MKSSFKTALCTVLCLCLTGAALAGCAQTGNEQARDLNGTTGDLN
                     GQYQRYGNNMNNGGYGNNLVGTEGNYTTQNGTYGRNMTSYRLDTMRNGTANNTTGNNT
                     GLNNTTGNNTGLNNMTGNGTANTTGGNDSRRASMIERQLENRNGIGDCVVLVNGDTAL
                     VGLRNATGNTANMSRLRSSIERRVKQIDSSIRNVMVTDSQDILTRMGRLGTAGNANGM
                     AANFMDEFNDLVERINGTIR"
     gene            complement(6989..8173)
                     /locus_tag="PLG72_05465"
     CDS             complement(6989..8173)
                     /locus_tag="PLG72_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ80281.1"
                     /translation="MTQLLLKLFVKDYKDTSDPSVREAYGKLSGITGIVTNLLLCAVK
                     ITAGVLAGSISIIADAVNNLSDSGSAIVTLISFKISGKPADAGHPYGHARMEYVSGLI
                     VSITIMFLGLELFLNSVRKIIEPQEMVFSWLIVLILAFSILLKLWLCLFYRKLGRAIN
                     SDAISAAMVDSRNDILATSAVLAALQVSGFTGFNTDGWMGAAVAVFITVSGIRLIKDT
                     VSPLLGMAPSKEMVDRIYRKIMSYDGIVGLHDLTVHNYGVGKYFASVHCEVSADRDIM
                     VGHDIIDNIERDFLKDLGIHLVIHLDPVITDNEKINNLKSAVDSLVRDISPEISIHDF
                     RVFFGITHSKLIFDVVVPYGFKYSDDELVGIISGKIREIDDNYRAVITVDHHYVPDFH
                     KE"
     gene            complement(8170..>10384)
                     /locus_tag="PLG72_05470"
     CDS             complement(8170..>10384)
                     /locus_tag="PLG72_05470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ80282.1"
                     /translation="VLLEAGDAVPADMRLIEAYSLKIEEASLTGESVPVEKDTAAMSS
                     ETGNVPLGDRRNMAYMGTTVVYGRGEGVVVATGMSTEMGKIADIITRTVDEKTPLQMT
                     LAGLSRVLTYCVLGISVFIFVLSVLENGGFSGTQVLDMFLLAISLAVAAIPEGLVAVV
                     TVVLSVGVTKMSKRNAIIRRLTAVETLGCTQVICSDKTGTLTQNKMTVTDTFGDKEKL
                     AISMVLCNDAELSGDKSDVTGEPTESALVRFAYKAGYDKSELESRMPRVAEAPFDSMR
                     KMMSTVHRDPSGRYVQYTKGAPDELLKKCSRMILEGEAVLLTDELREMILAENRKMAG
                     KALRVLGSAMKEMDSLPETVSPETLEQDLVFIGLAGMIDPVRPEVKDAIDKCRAAGIK
                     PVMITGDHRDTAVAIAKELGIIADEGQAVTGSVLDMIPDEEFTRDVGKYSVYARVQPE
                     HKVRIVEAWKNNGMVTAMTGDGVNDAPALKTADIGVGMGITGTDVSKNVSDMVLADDN
                     FATIVNAVEEGRRIYDNIRKAIQFLLSANLSEVLALFAATLLNFRLFDPIHILWINLV
                     TDTFPAISLGMEEAEPDVMKKPPRKAGENIFAGGMAAGVICQGIIVAILTLVSYSIGN
                     RQSHDIGMTMAFLTISLCEVFHSINMRSRVKSIFSLKTHNRYLSGAMVLSLLLTLSVI
                     YIPGVNTVFHLTALNAADLAIGAGLALLIIPVVELVKLAVSLKPAENAGIEGNRK"
BASE COUNT         2609 a         2790 c         2144 g         2841 t
ORIGIN      
        1 cagttctgcc gccgcgagcg agaaaaaaat ccacttcagt atcctcgcgc attctgatgc
       61 cgtcaaagca tcactgcggt ctgacaggcg gtctatcatc ctcgatattt cttcaccgct
      121 aatccctgac ttcagctcag ctccgcagtt gatacatttt ttagcattaa tgctgttttt
      181 gaaaccgcag gaatcacaca gcaaagttct cacctctctg aaatcgatgc gccgtgcaat
      241 actgacacga tagactctaa tacttctcca aattctttgt tggtccgggc ccaatgatcc
      301 aggtatctct ccttatagaa gttttttgcg gtttgcacgg cctccggaca atactggctg
      361 aaaacatcca ctactttatt agtttcgcca ttccatgccc tgagttcaaa atatgcctgc
      421 aacacgatct gcacaccctg cagattgcca ggtccgaact cgatctgcat attttttgat
      481 tttatatacg gatctgtttc ggcaaggtag actgccccgc aataaccatc ttttgcagca
      541 actcttactt tgcggatatt gctcatgacg atggttccga aacacatggg acagggttcc
      601 atgcatgtat acagcgtgta ctgtcccaaa ttcggatact ttgacgtatc aagtttctgc
      661 aggcattcag tttctgcatg ggcaatcctg ggattcaggg atccgggctc atacaggcgg
      721 tttctcccaa cagatatcac attaccgttt tcatcagcaa gagcggcgcc aatcgggatg
      781 gacccgttcc tgaatgattc ccatccctgt gaaaacgcgg ctttccaggg caactcaagc
      841 ttttcccaca tggatcctca ccctcacttt cagtcttgtt tcctatcgta atctgatttc
      901 aggaccgagt acatctcaag atccacgatc ccttttccgg tccaggacgc tgaatcggta
      961 caaatcgttg aatcccataa ttcctccaaa gtatacaaat catacttatc aagccgcata
     1021 ttatttacat ccaggcacct gcacggcagt gtattgccac ctttgcttaa gttcatcctg
     1081 tcatttgtac ggcaaagagt ctttcctcag ctcaagcgca gtatttgcaa tatttataat
     1141 aaattcatgc ttgccgtcta catatgaatc tatgtcatgc ctgtatttct cagcaagcga
     1201 ccgtttcagt gcggcgtatt catccctggc ttccttgtgc tcccgcaggt aatcccggaa
     1261 taaaatctgg gcaagcagca ccatgctgtc tttgggacat acatacatgt tgtaagccat
     1321 aaacccttcc cgcggaccgc ctttgaaccg ctcgcgtgta gggacgtccc cgtcgccgtc
     1381 atgctcgtat cccagttttt ttagctgttc ggcgatccgg ggaaaatcgt catatgattc
     1441 tataacgata tcgaaatcaa tgatcggctt tgccgcaaga ccgggaacac ttgtgcttcc
     1501 aatatgtaca atgtcgatcg caatatcttt gatatgcggc cataagaaat cgcgaatctt
     1561 tgcaaactcg ttcggccatt cggggttgta gggttctatg acaatagttc tcattttata
     1621 tccctccgtt cagatcagcg taaactaccc gcctgtcaag gtccaggcct tcggtccagg
     1681 caatttcaac gacgatatac ttcgatccgt ccagggaaac ataaaagctc tcataacctt
     1741 tatcggccaa acttttatcc aactgttccc tgatttcttc aaactgagaa gccgtgaaat
     1801 tttcgtcatc cgtactttta actgcataaa ctgcccgtac cccgccattt ttagccagcg
     1861 cacgggcaaa tgccctataa tccctgtcac ctgccgcata atagcttaat agggcaacac
     1921 agaacaatac caaaaggggt aatacggcag caatactttt tctcacggtc ttcaccacga
     1981 attgcatact gcagaagcca tttttgttct cacgggactt ccctgcggtt cccatgttta
     2041 tcatacctcc tcctcaatgc tatctctgtc ggtatggttg ctgccgccat cgggacacac
     2101 tggatcacac agatgaccgc gcctgctgtg ccaatcgtcc tttcatcctg ccccagcatc
     2161 atcagcacag ccggaacagt cagcaataat atgatccatc ctgaaacaaa ccatatcttt
     2221 cctgcatatc ggtgagcaaa atcccaggtg tccttgtttt tcacggacat cctggtcctg
     2281 tatccgtacc atgggtttat tttttcagga ggtttcctca taaaacttct gccaaaaccg
     2341 atcatcagca ccgggatcag catatccatt aacgtcataa aaagccagaa tcccattaga
     2401 ttgccctcca gattcagatt ttaacttacg gcagtcatat ttatatgaaa tatgccacga
     2461 aaaagaacct cgggaatctg aatatgatct ccccgttttt ctgtacaggg taactttttc
     2521 ttaaacgctc cataatatcg ttttcaaaat ccgcctgcat ctcatcatcc agtacattaa
     2581 ggtaaggcct cagtcctgtg ccgcgatacc attccaggat gtcgttgtaa cttttcatta
     2641 caggatagta tacggtttcc caaatggtaa agttatctgt gagttcagac aatatatcaa
     2701 aatattcacc aggagtaagt gtataataaa tccgggtttc agggaaaaac cttttccact
     2761 tgtcagaaaa gattcatcga tctctgccgt atccagaaac tctttttcga ttttgctcaa
     2821 atcgccttta aatcccagtt tccttaaaaa gtccagcgaa gacatttcac ccagatcagc
     2881 cttgtcccaa tacttgtata tttcttcaca gctcaagtcc ggaaaagcgc ggtttacaaa
     2941 gggcataaaa ccgcattccg ggtctttcat gatcaccccg tacatatcaa ggatcaatgc
     3001 tcgcataaaa tcctcctgta aaaaaacagt atttacaaat cagatagata tttatcattt
     3061 gcggaaaaca gcattatcct gccagtacct ttatcaacaa tccggctgat gtctaatatc
     3121 cgcgctctat tgccagaagt tcctccctgc cgatatatcc ggtacccgaa aaatcaagcc
     3181 ggacaatata cggcatccag aaccatatcc tcttgaaact gtcacagttg aaggccggat
     3241 cgtagtaatt cattatggta ctcaaagccg taccgtgcgt ccctataacg atgttctcat
     3301 ccttatgtgc ggctaatatc tcattcaaag cctcgatatt ccgcttctgg acgctggcaa
     3361 ggcattcacc gtccggctcg caaaagttaa aatcattcca gcgtttatgc aataaatcca
     3421 tgaaatctac gttcccgatc tcgccgggct gccgttccct gaacctttca tccaaataaa
     3481 tctctttgtt aaggataccg gcgcattcag atatggtatc catagctctt ttatatggac
     3541 ttgaataaaa gcaatctata tgcattttgg caagaagctc cgtaaccctt ttccggtcgg
     3601 ccaatccagt ctcagttaac ggccttgtcc tgtcatcctt ccaggactca tcaggctgtg
     3661 catgtcgaac gaaaaatata tgtgtcatat cctgacctcc gtagcttcga tttcagaatc
     3721 aatttcacaa accagcggca tatctgcatt gttttccaat actatacatt aaaaaacgca
     3781 aaaattccaa tagaataatt cacgccgatc aacacagttt atgaatcagg accagtcgtt
     3841 catacagaag agttcgggtt cccgctcatg tcttcagtat cttatcatca gtcaatccat
     3901 gtcaaactca tgcactgagc aatgaggtat tcctacggcc ccggtcagac aatggataac
     3961 catgccgtga gtaaccacta tgaccttctt atatgtggta tatcgggcta atacattcaa
     4021 cgccctggtt tttacatcgg tacgcaattc ccatctgaaa ctgccgtttt caggcaacaa
     4081 tccgttattt ttctgctcct cggaagctat tcgctttaag tcctcatggc tttttacctg
     4141 ataggacaaa tccagttcgt gctcccgcaa atcaatttca accctgatat ctataccaag
     4201 attttttgaa agaattgccg cagtttgcag cgctctcgta taaggcgaag tgatgatcag
     4261 atcgcaccct gcaagccttt tatccttact gcattcaaca gcctgcctga taccatcttc
     4321 agtcaacgga gccaattcaa gcccatgacc gatgaaattc ctttcttcca cgggtttgta
     4381 tgtgaactcg ccatgcctta ccaggtaaaa acgtgccata cgaccaccac ttttcggacg
     4441 gagaactgcc ttacaaatat tctaatccat tccttcgaca gattcaatca gtttcgccgg
     4501 gtcgactacc cgccctgctg catcgccgct gccgtcgtca ccgcacaggg cggaactcgt
     4561 ctcaaacgct ttttcccata attcttccgg tgtgatatca ggtttgacct ggcacgccag
     4621 tgcatacaac cctgcacata tggcactgcc cagccggtta ttccccgttt atgcagttct
     4681 ctcatgccca aaccgggatt ttcccccagt tccatgatca tttgcgggtc aaagccatcg
     4741 ggaagcatgt cgggccattt ggttttgccg gaggtgaaca aaatgtaaaa aggagggtgt
     4801 acaaaaatcg aattgttgtg tgtaaattac agtaaaactc ttgttggttc tagtgtgcta
     4861 ttttacataa tatatgatat ctcatcatta tgtaatccgc ttattgaaat aatttaataa
     4921 attatattgc aacaattcga tttttgtaca ccctgctcat tatttcctat agaaataaat
     4981 gtctgtaacg aaagccgaca actatttagc agaatacgat actttatgca cagaagccag
     5041 cacttgaaca catagaagca agtaccttcc tgcgcagaca acagtttact ttgcacgcgc
     5101 ctggcgctct gcgtcgagca tatgcatggt accttgcaca gcagatatcc ggcgtcttta
     5161 tgcgcccaga tgttctatca gtatcttgac gcccatggcg atcaatatta taccgcctgc
     5221 tgtttcagcc cgggatttga attttgcgcc aaaaatgctg cccactttta caccgatggc
     5281 cgacagtatg aacgtagtca cccctatgat cgaaatagcg cggatgatct cgaccttcag
     5341 aaaagcgaaa gatacgccca cggccaatgc atcgatactg gtggccactg aaaacatgag
     5401 catatttttg agatcgtatg aattgccggt gtttgatccg tgtggattgt cacaactgtc
     5461 gtgccttgat tcccggatca tgttgagccc gatcaaagag agcagtgcaa atacgatcca
     5521 atggtccact gccgtaatat aatcgctgaa ctgtgcaccg agcagataac cgacaagtgg
     5581 cataaatgcc tggaatcccc cgaaaaacaa accagtcaca gccgcgtgcc tgaatttgag
     5641 ttccatcgtc gccaggcctt tgcataccgc tacagcaaaa gcgtccatcg aaagtccgac
     5701 agccactaaa aacaactcaa tcatgcccat cagtatatgc ctgcctctca gtcagcgaag
     5761 ataataaccg aaccataata gcatatatct caaaacccct tattattcta aaatatcgca
     5821 tcaaattaaa agtaattgca ggcatagcct gcaattaccc ggccgcggca gcccctgccg
     5881 ggactgccgt cctggacctg atggagtata tggcggaaac tgctgatgag atcacacagt
     5941 tccctgcccc atgttaccgt atagtgccgt ttatcctctc gaccagatca ttgaactcgt
     6001 ccatgaaatt ggcagccatc ccgttagcat taccggctgt gccaagcctg cccatacgag
     6061 tcagtatgtc ttgcgaatcg gtcaccatga cgttccttat agaactgtcg atctgcttga
     6121 cccttctttc gatactggac ctcagccttg acatattggc ggtattgcct gttgcgttcc
     6181 tcaggccgac aagcgcagta tccccgttaa cgagaaccac acagtcgcct atgccattcc
     6241 tgttctccaa ctgcctttct atcatagatg ctcttctgct gtcattcccg ccggttgtgt
     6301 tggcagtgcc gttgccggtc atgttgttga gtcccgtgtt attcccggtc gtgttattaa
     6361 ggcctgtgtt gtttcctgtc gtattgttgg ctgttccgtt cctcattgtg tccaggcggt
     6421 aactcgtcat gtttctgcca taggtgccgt tttgcgtcgt atagtttcct tccgtcccta
     6481 ccaggttgtt cccataacct ccattattca tgttgttacc gtatctctgg tactgtccgt
     6541 tcaggtcgcc ggtcgtaccg ttgagatccc gcgcctgctc gttacccgtc tgggcgcagc
     6601 ccgccagcgc agcaccggta aggcataaac acaaaacagt acataaagct gttttaaatg
     6661 atgatttcat ttcaacacct cccgtactat tctgtgtaca taattcattt ttatttgagt
     6721 taaaaattaa taaagttgca taatcctcca cgagcaggaa tatataatat ttacataaaa
     6781 atgattttat gttgctgtaa atatgtcaga aaagtaataa gaatacagaa aaacataaaa
     6841 gacggtataa aagcagcata tgatacaaga catccgcagt tatccgaaaa caacgatcgc
     6901 agcggctcat atggaaagcc gtcctttgtg aaaaaaatgt tatatatgga aagagtctct
     6961 gtatagagct cttccttagt atatagagct attccttatg gaaatccgga acgtaatgat
     7021 gatccactgt tataactgcg cggtaattat cgtctatctc ccttattttt cccgaaatga
     7081 tccccacaag ttcatcgtcg ctgtatttaa aaccgtacgg gaccactacg tcaaatatca
     7141 gttttgaatg ggtgatgccg aaaaatacgc ggaaatcgtg tatgctgatc tcgggtgata
     7201 tgtcccttac cagcgaatct acagccgact tcaggttatt gatcttctcg ttgtcggtta
     7261 tgaccggatc aaggtgtatg accagatgta tgcccaggtc tttcaggaag tcccgttcta
     7321 tattgtctat gatgtcatgt ccgaccatta tgtcccggtc ggcggacact tcgcaatgca
     7381 cagatgcgaa atatttaccg acaccgtagt tatgtaccgt caggtcgtgc agcccgacga
     7441 tcccgtcgta gctcatgatc tttctgtaaa tacggtccac catttctttg gaaggcgcca
     7501 tgccaagcag cggacttacc gtatctttta tgagccttat gccggatacg gttatgaaaa
     7561 cagcaactgc tgcgcccatc cagccgtcag tattaaagcc ggtgaatccg gaaacctgca
     7621 aagcggcgag gacagctgag gtcgcaagga tatcgttcct gctgtccacc attgcggcgc
     7681 taatggcgtc tgagtttata gccctgccaa gtttcctgta aaaaaggcag agccacagtt
     7741 taagcagtat cgagaaagcg aggataagga cgataagcca tgaaaatacc atctcctggg
     7801 gttctattat cttcctgacc gagttcagga acagttccag tcccaggaac ataatcgtta
     7861 tcgatacgat aagccccgaa acatactcca tcctcgcatg cccgtatgga tgcccggcat
     7921 ccgcaggctt tcccgatatc ttgaagctga taagcgtcac gatggcagat cccgaatcag
     7981 acaggttgtt gaccgcatct gcgataatgg atatgctgcc cgccaggacg cctgccgtta
     8041 tttttactgc gcacagcaac aggttggtca cgataccggt tatgcctgac agctttccat
     8101 acgcttcacg cacacttgga tcggatgtgt ctttgtagtc tttcacaaat aacttaagca
     8161 acaattgtgt catttcctgt ttccctcgat tcctgcattt tccgccggct ttagcgaaac
     8221 agccagcttt accagttcca caacaggtat gatcagaagc gccagcccgg cgccgatggc
     8281 gagatcggct gcgttgagcg cggtcagatg gaataccgtg ttgactcctg gtatatatat
     8341 cactgacagc gtcaaaagca gcgacaggac cattgcgcct gaaagatacc tgttgtgcgt
     8401 tttgaggctg aatatggact tgaccctcga ccgcatgttt atagaatgga agacttcgca
     8461 aagcgaaatg gtcaggaacg ccatagtcat tccgatatca tgggactgcc ggttccctat
     8521 gctgtaagag accagcgtca gtattgcaac gatgatcccc tggcagatca ctcccgcagc
     8581 catcccgcct gcgaaaatgt tttcgccggc ctttcgcggc ggctttttca taacgtccgg
     8641 ctcagcttcc tccataccaa gggatatagc cgggaacgtg tccgtcacga gatttatcca
     8701 caatatatgg atcgggtcaa aaagcctgaa attcagcaaa gtcgctgcaa acagcgccag
     8761 gacttcgctc aggttggcag aaagcaggaa ctgtatcgcc ttcctgatgt tgtcatagat
     8821 ccggcggcct tcctccaccg catttacgat agtcgcaaag ttgtcatcgg caagcaccat
     8881 gtcggaaacg ttctttgaca catctgtccc cgttatgccc atacccacgc caatatctgc
     8941 agttttgaga gccggcgcgt cattgacgcc gtcgcccgtc attgccgtga ccatgccgtt
     9001 attcttccat gcttccacta tacgcacctt gtgctcgggc tgtacgcgcg cataaacgct
     9061 atatttcccg acgtccctgg taaactcttc atccggtatc atgtcgagta cggaacctgt
     9121 gaccgcctgg ccttcgtcag caatgatgcc cagttctttc gctatcgcaa cagccgtatc
     9181 ccggtggtct ccggtgatca tgaccggctt gatcccggca gcgcggcatt tgtcaatggc
     9241 atccttcact tcggggcgca ccggatcgat catgcctgca aggcctatga ataccaggtc
     9301 ttgttccaga gtctcaggcg aaacagtttc gggaagggaa tccatctcct tcattgcaga
     9361 gccgagcaca cggagagcct ttccggccat cttcctgttt tctgcaagta tcatctcgcg
     9421 cagttcgtcg gtcagaagta ccgcttcacc ctccaaaatc atacggctgc attttttgag
     9481 cagttcatca ggcgcgccct tggtatactg gacatatctg cctgacggat ccctgtgtac
     9541 cgtggacatc atcttccgca tggaatcgaa aggagcttcg gcgaccctcg gcattctgct
     9601 ttcaagctcc gacttgtcat agcccgcctt atacgcaaat ctcaccaggg cgctttcggt
     9661 gggctctcct gtcacatcgc tcttatcccc ggaaagctcg gcatcgttgc acagtaccat
     9721 ggatattgcg agtttttcct tatccccgaa tgtatctgtc acagtcatct tattctgtgt
     9781 caatgttcct gtcttgtccg agcatatcac ctgtgtacag ccaagtgttt caactgcggt
     9841 cagccttcgg attatagcgt tgcgcttcga cattttggtg acaccgacgg acagtactac
     9901 agtaaccacc gcgacaaggc cttcaggtat cgcggctact gccaggctga tcgcaaggag
     9961 gaacatatca agtacctgcg tgcctgaaaa acctccgttt tccaatacgc tgagcacgaa
    10021 tataaaaacg ctgatcccga gcacacagta ggtaagcaca cggctgagtc cggccagcgt
    10081 catctgcagc ggagtctttt catccacggt cctggtgatg atatcggcta tcttgcccat
    10141 ctcggtcgac atgccggtgg caacgacaac accttcgccg cggccgtaga ccacagttgt
    10201 ccccatataa gccatgttgc gcctgtcgcc aagcggcaca ttaccggttt ccgacgacat
    10261 tgccgccgtg tctttctcca ccggtaccga ttcgcctgtg agcgaagctt cctcgatttt
    10321 caggctgtac gcttctatca gcctcatatc tgccggaacc gcatctccgg cttccagcag
    10381 gact
//
LOCUS       DAOSCC010000074        10188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4328_length_10188_cov_5.114083, whole genome shotgun sequence.
ACCESSION   DAOSCC010000074 DAOSCC010000000
VERSION     DAOSCC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_10188_cov_5.114083"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLG72_05475"
     CDS             <1..297
                     /locus_tag="PLG72_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80283.1"
                     /translation="NTSWSNKKLNKTNSTTEINVDADLPFLGINGLSAAMSIAGEDVF
                     GIDDFSLPDFDSAAVMDLYAASDEDMDKLEMEIMASFGTFYLNNKYIFDALLGW"
     gene            306..1523
                     /locus_tag="PLG72_05480"
     CDS             306..1523
                     /locus_tag="PLG72_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80284.1"
                     /translation="MTSFFALLKNDIRLFLRDWKACVLLLIAPLFFISFFTYALSPYL
                     EESSFIEPFPIAVVDKEDTTQTRMLINQVEEIGIFSEVIRMDEEEALRSLADKEVGGV
                     IIIPEDFTNSVSMGENKPVTVVGNGAMPLQAYVVKNIVQSASNLVTAAQGAINTIWHY
                     DTEAGLSDSERDERFNEAVMDYMLAALARTSIFVSEDAVSEYSVTPAEYFTAALIIVF
                     MMFAGMPGMKMLVTERTSGITSRISAAPVRVWKVVLSKLLVSVMLLVIQFGIIIFMTS
                     KIFNNYWGAPVKDVLILFGGIILAVSAWSVFTASVSPTPASADIIGNLGILLMAVLGG
                     SIYPLTSMPEGIRRISVLTINRWAMDGFMVLFSGSSVTDTSTHALALSAAALALFAAS
                     ALIMRFAGRRGAK"
     gene            1529..2728
                     /locus_tag="PLG72_05485"
     CDS             1529..2728
                     /locus_tag="PLG72_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80285.1"
                     /translation="MSVTFYKLKMMLADKLFFAAMIIIPMLITVAAGYALRYEKLNTI
                     PVAIVDEDMSSSSQLLTDRLKGKEGITLHITGREEAERMLENSEVEQVYIIKPGFGKA
                     IERGESDGLIEVYSAPSSYSADFTREVVAGEAFRLIMNNAAANEVTGKYDELGIDMGS
                     GFRDEVIAYADSFWEPEPLLTVDYRELKGSGAEPVDRVSMPAATASSAGLITAFIMFF
                     MLFGSGWLVEERTNGTIKRLGAGHGAVAVSFAGSILALFAAGILQIVMFGAILKLFFG
                     IVLFNGVLSYITLLSYLLAVVAISMFLSSVLKTQAQLQAGAPVIALLAGFMGGCFWNF
                     VEMPERIRFLSLLTPQGWALNALNGLLLDASDTSAMLLPVSVLLGVSLILLPASYIII
                     KIQLNRG"
     gene            2955..3626
                     /locus_tag="PLG72_05490"
     CDS             2955..3626
                     /locus_tag="PLG72_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80286.1"
                     /translation="MDNLKNKLLDILLFPARLYEKLSDRKATLAAGIVLVGVIDLFLP
                     DVMYIIKKLFFGKTLFDIIYNAGMAVLVLLLLGFIDVICMSVPLFDISVYLKRKEAQF
                     IMNTGIGAKEQQPALDPSAIKVMKAYIMSHFIMVPVSIIFNYVLSLDVFGENPAFLQT
                     LAGILFIVIMIWSAAIMTRGINSLFRFSILFRRLVFMIVFAWNSLFGMVFDIMIVDWL
                     MRLFR"
     gene            3971..4987
                     /locus_tag="PLG72_05495"
     CDS             3971..4987
                     /locus_tag="PLG72_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80287.1"
                     /translation="MPGTAGDPENTAGKGELQEAEQGAAAPEIGDNAGNLSGQAGSQD
                     AEQDTVVPETSDNAGNTGNQQEPQRTGKEGNIPETADDGQTPAEPEDPNETDQVSDDL
                     NAVDRIRNALKDIDSPAVTDELVRLFFDYQGESHRYKGPKHRYELRLLPEFGPGKTLD
                     WDGLTVFIFFMSNHLEHDQDEEGSVFFSRELFDETARRLLPGLKYKHRRSAYFYYENG
                     VYKATGWDMHGGVYYRLKSISRDDKGTFTASFDGFVFHENDYFGAPYDMISENMKAMY
                     DYVGGKRADTADEDEVLLEIFLRDDYDEILHVDQHLEITFRISSDPLFAFEYLSCTRE
                     YLGQ"
     gene            5634..7286
                     /locus_tag="PLG72_05500"
     CDS             5634..7286
                     /locus_tag="PLG72_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOJ80288.1"
                     /translation="MEGPKEAGPMVRPEYSEIVRLAKEFDVVPVCREIYADITTPIAI
                     LRTISRSSRRYFLLESIEGGEKWGRYSFLGFDPIARITCKNGEITIEESPGPHSGTSH
                     SRRKTMYSEKPLEVLRSYLSQYRAPVLADIPPFAGGFVGYFSYEMIGYAEPVLKLRKS
                     GFCDFDVMLCDKVVAYDHLKQKISVVVNMRTDNVEENYSKALSDIADMVRLLKEEAPA
                     AGKAGTDRIEGAGCDNAAEIGYGSAVTADEASAGPDSSAGSMANRNAETAAARPVFTC
                     NTTKEEFCGMVEKAKEYIRNGDIFQAVISRRFETEYRGSLLNAYRVLRTTNPSPYMVM
                     MQIDDIQLISTSPETLVRLKNGVLTTFPIAGTRPRGKDTCEDKKLEEELLADEKELAE
                     HDMLVDLARNDLGRISKYNSVHVINYKMILRYSRVMHIASEVESLIRDDKDAFDAIEA
                     LLPAGTLSGAPKIRACEIIDELEKDPRGIYGGAIGYIDFTGNMDACIAIRMAVKKGDR
                     VYVQAGAGIVYDSIPEREYEESGNKARAVMEAIIGASEVDER"
     gene            7283..7864
                     /locus_tag="PLG72_05505"
     CDS             7283..7864
                     /locus_tag="PLG72_05505"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOJ80289.1"
                     /translation="MILIIDNYDSFTYNLLQLAGSFDPDIKVIRNDGMSVGDIINMRP
                     SRLIISPGPGYPKDAGICRELILGVRGMMPILGICLGHQVICEAFGAAVVPARQPVHG
                     KRRDIHIANGSSIFKGLPPVIQAGRYHSLTVRREDLPEDLLVIAEDSEEEIMGVKHRD
                     HEIYGLQFHPESILTPHGAVIMSNFLKIGGDCI"
     gene            7861..8874
                     /gene="trpD"
                     /locus_tag="PLG72_05510"
     CDS             7861..8874
                     /gene="trpD"
                     /locus_tag="PLG72_05510"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOJ80290.1"
                     /translation="MIREAIQKLLNNEDLSFEMTEAVMDEIMSGSATNAQIASFITAL
                     RMKGETIDEISACASAMRKHGAKLEYDGDLLDIVGTGGDQAYTFNISTISSFVISAAG
                     VPVAKHGNRSVSSKCGSADVLEALGVRIDIPVSKSERILKEIGLCFMFATRYHSSMKY
                     AAPVRKEIGARTVFNILGPLANPANANHQLLGVYDEGLVEPLARVLAKLGVKKAMVVH
                     GLDGLDEISLAAKSVVCEVNNGTLNSFFFDPRQFGFEYCRPEDLVGGGPEENADIARR
                     VLGGERGPRRDTVLLNSAVCLYMFFDNVTLRECVRIAADMIDSGRAMQQLNRFVELSN
                     APD"
     gene            9154..10083
                     /gene="trpC"
                     /locus_tag="PLG72_05515"
     CDS             9154..10083
                     /gene="trpC"
                     /locus_tag="PLG72_05515"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOJ80291.1"
                     /translation="MILDTLAASARLRVEAAKMQMPLDELKRQVARMDEITGRDKPGY
                     LPNQNISDHIEAKQHAGHKAAVHIEPQHGVLEPFAFEKALKGSGISFICEVKKASPSK
                     GIIAQDFPYARIACEYAEAGADAVSVLTEPGYFLGSDAYLEEISKAVEIPVLRKDFIV
                     DEYQIWQSKLIGADAVLLICALLDTEALDRFIGICDELGLSALVEAHTGDEVRSALEA
                     GARVIGVNNRDLRTFEVDINNCIKLRDLVPEGVIYVAESGINTREDILMLEKAGVDAV
                     LIGETLMKSRDRRSMLPYLRGDTAVKGKAGGKG"
BASE COUNT         2640 a         2307 c         2979 g         2262 t
ORIGIN      
        1 aacacgtcct ggagcaacaa gaaactgaat aagaccaaca gcaccaccga gatcaatgtg
       61 gatgcggatc tgccgttcct cggtattaac ggcctttccg ccgcaatgag catagccggc
      121 gaagatgtct tcggcataga cgacttctct ctgccggatt tcgacagtgc ggcggtcatg
      181 gacctgtacg ccgcttcgga tgaagatatg gataagcttg agatggagat aatggcgtcc
      241 ttcgggacat tctatctcaa caacaagtat attttcgacg cgctgctggg atggtgaggc
      301 gcagcatgac ttcgtttttc gcgctcctca aaaatgacat aaggctcttc ctcagggact
      361 ggaaggcatg cgtccttctg ctcatagccc ctttgttttt catatcgttc ttcacatatg
      421 cgctgtcgcc gtacctggaa gagagcagct tcatagagcc gttccccatt gccgtggtgg
      481 acaaggagga tacgactcag acgaggatgc ttataaacca ggtcgaggag atcggcatat
      541 tcagtgaagt cataaggatg gacgaagaag aagcgctgcg cagcctggcc gacaaagagg
      601 tcgggggcgt catcatcata ccggaggatt tcaccaacag cgtttccatg ggcgagaaca
      661 agcccgtcac cgttgtcggg aacggggcga tgcctctcca ggcgtatgtc gtgaaaaaca
      721 tcgttcaaag cgcctccaat cttgtgacgg cggcccaggg cgcgataaat accatctggc
      781 attatgatac ggaagccggc ctttccgaca gcgagcggga cgaaaggttc aatgaggccg
      841 ttatggatta catgcttgcg gctcttgcaa ggacatccat atttgtttcc gaggatgcgg
      901 tcagcgagta cagcgtaacg ccggcggagt actttactgc cgcccttata atagtgttca
      961 tgatgtttgc aggcatgccc ggaatgaaaa tgctcgtaac ggagagaacc agcgggatca
     1021 cctcgcgaat ttcggcagcg ccggtgcggg tgtggaaggt ggtcctgtca aagctgctgg
     1081 tttccgtcat gcttcttgtg atccagttcg gcatcataat attcatgacg tcgaaaattt
     1141 tcaacaacta ctggggagcg ccggtaaagg acgttctgat acttttcgga gggatcatcc
     1201 tggcagtcag cgcatggtca gtgtttacgg cgtctgtttc gccgacgccg gcttcggccg
     1261 acattatcgg gaatcttggc atactgctga tggctgtgct gggcggcagt atatatccgc
     1321 tcacatcgat gcctgagggc atccgcagga tcagcgtact tacgataaac aggtgggcaa
     1381 tggacgggtt catggtcctg ttctcgggca gcagcgttac cgatacctcc acgcatgccc
     1441 tggcgctttc cgcggcggcc ctggccctgt ttgccgcatc tgccctcata atgcggtttg
     1501 ccggaaggag gggagcaaaa taagactttt gtcagtcaca ttctataaac tgaaaatgat
     1561 gctggcagac aagcttttct tcgcggcgat gataatcatc ccgatgctga taaccgttgc
     1621 ggcaggatat gcgctcaggt atgagaaact gaacaccata ccggtcgcca tcgtggatga
     1681 ggatatgagc agcagttcgc agctgctgac agacaggctg aaagggaagg aaggcatcac
     1741 ccttcacatc accggcaggg aagaagcgga gaggatgctg gaaaacagcg aggtggaaca
     1801 ggtatatatt ataaagcccg ggttcgggaa agctatagag cgcggggaaa gcgacgggct
     1861 gatagaagtg tacagtgcgc cgtcgtcata ttcggccgat ttcaccaggg aggtcgtggc
     1921 aggcgaggcg ttcaggctga taatgaacaa cgcggctgcc aacgaggtga caggaaagta
     1981 cgacgaactg gggatcgata tggggagcgg cttcagggat gaggtaattg catacgccga
     2041 ttccttctgg gagccggaac ccctgctgac cgtagattac agggaactca aggggtccgg
     2101 cgccgaaccc gtggacaggg tatcgatgcc tgctgcgact gcatcatctg cagggctgat
     2161 cacggcattc ataatgtttt tcatgttgtt tggaagcggc tggcttgtgg aagagaggac
     2221 caacgggacg ataaaaagac tgggcgccgg gcacggagca gtggctgtat cattcgcagg
     2281 cagcatcctt gcgctgtttg cagcgggtat cctgcagata gtgatgtttg gcgccatcct
     2341 gaaactgttt ttcggcatag tgctgttcaa cggcgttctt tcctacataa cgctgctgtc
     2401 atacctcctt gccgttgtcg cgatcagcat gttcctttca tctgtactga aaacacaagc
     2461 gcagctccag gcaggcgcgc ctgttattgc attgcttgcg gggttcatgg gagggtgctt
     2521 ctggaatttt gtcgagatgc cggaaaggat aaggttttta tcgctgctga cgccgcaggg
     2581 atgggctctg aacgccctga acggactgct gctggacgct tccgatactt ccgcgatgct
     2641 gctgccggtg tcggttcttc tgggcgtgtc attgattttg ttgcctgcca gttatattat
     2701 tattaaaata cagttaaaca gaggttagca ggaacgggac gcctaagcaa agacaagccg
     2761 ggacgggccg gtgccgggac ggcggggcag gacgggcgga taagggcgga agcggacagg
     2821 tacttttgag tacaagtaat aaaggtggcc gcagcgaggc caccaaatgg atgacccgga
     2881 cctgcagcag tcggactgga actaaaatta ccaaattggt gtcattatat atacagtaat
     2941 gcgaaggaga aattatggac aatctgaaga acaaattgct tgacatattg ctgtttccgg
     3001 ccaggctcta tgagaagctc agcgacagga aggcaacgct ggcagcggga atcgtgctcg
     3061 taggagtgat cgatcttttt ctgcctgatg tgatgtatat cataaagaaa ctttttttcg
     3121 gaaaaacgtt attcgacata atatataatg ccggcatggc tgtcctggta ctgctgctcc
     3181 tgggctttat cgacgttatc tgtatgagcg tcccgctttt cgatatctct gtatatctca
     3241 agaggaaaga agcccagttc attatgaata caggcatcgg agccaaagag cagcagcctg
     3301 cgctggatcc ttccgctata aaggtgatga aggcatacat aatgtcgcat tttataatgg
     3361 tcccggtgtc gataattttt aactacgtgc tttccctgga tgttttcgga gagaatcctg
     3421 cgtttttgca gaccctggca ggcatattgt tcattgtcat aatgatatgg tctgcggcta
     3481 taatgacaag agggatcaac tcgttattcc ggttcagcat cctgttccgg agactggtct
     3541 tcatgatagt atttgcatgg aactcccttt tcggtatggt tttcgacatc atgatcgtcg
     3601 actggctcat gcggttgttc aggtgacgga cagaacttca aatgatgaca aacagtggtt
     3661 ttgttgcggc agggctattg gcgtctgctg gatggatcct tataaaatca ttaggctgcg
     3721 gatggtgatt tatatgggta aatttttgcg cccaaaacag gttattacag cgacggtcat
     3781 taaacgatca gctttattat tggcgatatt gccggtcctg tgcctgcttc cgtcatgtat
     3841 gccgtcagat agggcagaca tcgggataaa tatcgaacat gaagatatta ttgattggcc
     3901 tcggcccggc gatggtgagg agatttccgc cggccaggat gatcagcagg aagcggggca
     3961 ggataccggc atgccgggga ctgccggcga tccggagaat actgcgggca agggagaatt
     4021 acaggaagcg gaacaaggcg ccgctgcacc ggagatcggt gataatgcgg gaaatctttc
     4081 cggtcaggca ggatctcagg atgcggagca ggatactgtt gtaccggaaa ccagcgataa
     4141 tgcgggaaat actggcaatc agcaggagcc ccagagaact ggaaaagaag gaaatattcc
     4201 ggagactgcc gatgacgggc aaactcctgc ggaaccggag gatccgaacg aaacggacca
     4261 ggtatcagat gacctgaacg ctgtggacag gatccgaaat gcgctgaagg acattgactc
     4321 ccctgccgtg accgatgagc ttgtcaggct gtttttcgat tatcagggtg aaagccacag
     4381 atataaaggg cccaaacacc ggtacgagct gcgcctgctg ccggaattcg gaccggggaa
     4441 aactctcgac tgggacgggc ttaccgtatt catatttttc atgtcaaacc atttggagca
     4501 tgatcaggat gaagagggat ctgttttttt cagcagggaa ttgtttgacg aaacagcccg
     4561 ccggctgctt cccgggttga agtataaaca tcggaggagc gcctattttt attatgagaa
     4621 cggggtatat aaggctaccg gctgggatat gcatggaggc gtctattacc gcctcaaaag
     4681 catttcaagg gatgacaagg gaacttttac cgcatccttt gacggattcg tgttccacga
     4741 aaacgattat ttcggagcac cgtacgatat gatctcggaa aacatgaaag ccatgtatga
     4801 ctacgtgggc ggcaaaaggg ccgatacagc agacgaagac gaagttttgc tggagatatt
     4861 cctgcgtgac gattatgatg aaatacttca tgtggaccag cacctggaga taacgttcag
     4921 gatttcatcc gatcctttgt ttgcttttga gtacctgtca tgcacacggg agtacctggg
     4981 gcagtgagca gcggggcaat ggaatgacat tcggaacaat atgatattaa acattgaggc
     5041 aatggtatat tcgaacaatg gtgagttccg cttttgtcat atgttatgcc ggtatgcaac
     5101 tttttattga caatgacaat aaccgctgtt ataatttttt ccaacacata aatacatact
     5161 taacaaaccg aggagcaaga cgagtagcag tgatctcctg tatccagaaa gccgccggcg
     5221 atgggaatgc ggctgcagga ccattgtgaa tggacttgtg agggaagcct gaaatcctgg
     5281 gagagtaggc gcttacgggg gtccgacccg ttacaagcag gatgcatatg atggtatgca
     5341 tgaatgagtg ggcgcatcat gcgtcaatat gggtggcacc gcagaagctt gagcttttgt
     5401 ccctgtttgg gacaaaagct tttttatttt gtacatgcaa ttgcatgtac gatttccgcc
     5461 cggcggattt gtgccggaaa tccagcgtca ttcataacaa cgggcggata tggggcagaa
     5521 acacttgcat tatacataac gacaggcaga tatgggccgt aaatacctgc gccatccgaa
     5581 aagatgggca gatatgacat tcatatatcc aagtcatgga aagaggtgat tttatggaag
     5641 gaccgaaaga ggcgggcccg atggtaaggc ctgaatacag cgaaatcgta cggctggcga
     5701 aggaattcga tgttgtaccc gtctgcaggg agatctatgc cgatatcacg acgccgatcg
     5761 caattttgag gacgatctcc cgatccagca gaagatattt ccttcttgaa agcatcgagg
     5821 gaggggaaaa atggggaagg tattctttcc ttgggttcga tccgatcgcc cgcatcacgt
     5881 gcaaaaacgg ggaaataacg atcgaagagt cccctggtcc gcacagtgga acttcgcatt
     5941 cacgcaggaa aaccatgtat tccgaaaaac ctttggaagt gctgagatct tacctttcac
     6001 agtacagggc gcccgtcctg gcggacattc ctccgtttgc cggcgggttt gtcggttact
     6061 tctcatatga gatgatcggc tatgcagagc ctgtcctgaa actcagaaaa agtggattct
     6121 gcgattttga cgtcatgctg tgcgacaagg tggtcgcata cgaccacctg aaacagaaga
     6181 tcagcgtcgt tgtgaatatg cggacagaca acgtcgaaga gaactactca aaagcacttt
     6241 cggatattgc ggatatggtc aggctgctga aggaagaggc accggcagcg ggaaaagccg
     6301 ggacggacag gatagaagga gccggatgtg ataacgctgc agagattgga tatggcagtg
     6361 cggtgacagc ggacgaggca tcagcaggac cggacagttc cgcgggatcc atggcaaaca
     6421 gaaatgcaga gactgctgca gccaggcctg tttttacctg caacaccacg aaagaagaat
     6481 tctgcggcat ggtcgaaaag gcaaaggagt acatcaggaa cggcgatata ttccaggcgg
     6541 tcatatcaag acgttttgag acggaataca ggggcagcct ccttaatgcc taccgtgtcc
     6601 tgaggacgac caacccgtca ccgtacatgg tgatgatgca gatcgacgac atccagttga
     6661 tcagcacatc gccggaaaca ctggtacggc tgaagaacgg cgttcttacg acattcccca
     6721 tagcgggcac gagaccccgc ggcaaagaca cctgtgaaga caagaagctt gaggaagaac
     6781 tgctggcgga tgaaaaagag cttgctgaac atgatatgct ggtcgacctt gcaagaaatg
     6841 acctgggcag gatctcgaag tacaacagtg tgcatgtgat aaattacaag atgatacttc
     6901 ggtattcacg ggtcatgcac atagcttcgg aagtggaaag cctcatcagg gacgataaag
     6961 atgcgtttga tgccatagaa gcgctgctgc cggcggggac attgtctgga gcgcccaaga
     7021 tccgcgcatg tgagatcatc gatgaactgg agaaggatcc gaggggcata tacggaggag
     7081 ccataggata tatagacttc accggcaata tggacgcttg catcgcaatt cgaatggctg
     7141 tcaaaaaagg agacagggtt tatgtccagg ccggtgcagg gatagtttac gacagcattc
     7201 ccgaaaggga gtatgaagag tccgggaaca aggccagggc tgtaatggag gccattattg
     7261 gagcaagtga ggtggatgag cgatgatcct gattatcgac aactacgaca gcttcacgta
     7321 caacctgctg cagcttgcag gttcattcga ccctgatata aaggtgatcc gaaatgacgg
     7381 gatgtccgtc ggcgacatca taaacatgcg cccgtcccgc ctgatcattt ctcccggacc
     7441 gggatatccg aaagacgctg ggatctgcag ggaactgatc cttggggtca ggggcatgat
     7501 gccgatcctt gggatatgcc ttggccacca ggtcatatgc gaagcgttcg gagcggccgt
     7561 cgtgcccgcc aggcagccgg tacacgggaa acggagggat atccatatcg caaacgggag
     7621 cagtatattc aaaggcctgc cgccggtgat acaggcgggc agataccatt cccttacggt
     7681 ccggagggaa gaccttccgg aggacctgct cgtgatcgct gaagacagcg aagaggagat
     7741 aatgggggta aagcacaggg atcatgaaat atacggcctc cagttccatc ccgaatcgat
     7801 actgacgccg catggagctg tcataatgag caactttctg aaaatcggag gtgattgcat
     7861 atgatcaggg aagcgataca aaagctgctg aacaatgagg acctgtcctt tgaaatgacc
     7921 gaagcggtca tggacgagat catgagcgga tcagcaacaa atgcgcagat agcttccttt
     7981 ataacggctc tgaggatgaa aggcgagacc atcgatgaaa tttcggcgtg tgcatcggca
     8041 atgaggaaac acggcgccaa gctggaatac gacggagatt tgctggacat cgtcggtacc
     8101 gggggcgacc aggcatatac gttcaatata tcgaccatat catcatttgt aatatccgct
     8161 gccggtgtgc ctgtggcaaa gcacggcaac aggagcgtgt cgagcaaatg cgggagcgcc
     8221 gatgtgctgg aagcgctggg ggtaaggata gacatcccgg tctcaaaaag cgagaggatc
     8281 ctgaaggaaa tcgggctgtg cttcatgttc gccacccgct accattcgtc catgaaatat
     8341 gcggcgccgg taaggaaaga aatcggggcg cgtaccgttt tcaacatcct ggggccgctc
     8401 gcgaatcctg ccaacgcaaa ccaccaactg ctgggcgtat atgacgaggg cctggtggag
     8461 ccacttgcaa gagtactcgc aaagcttggc gtgaaaaagg cgatggtggt gcacggactt
     8521 gacggcctgg atgagatatc gctggctgca aaatccgttg tctgcgaagt caataacggg
     8581 actctgaaca gcttcttctt cgatccgcgc cagttcgggt tcgagtattg caggcctgaa
     8641 gaccttgtcg gaggagggcc ggaagagaat gcagatatcg caagacgcgt actgggcggc
     8701 gagaggggtc ccaggaggga cacggtgctt ctgaattcag ccgtatgcct ctatatgttc
     8761 ttcgacaacg tgacactgag ggaatgcgtg aggatcgccg ctgatatgat agacagcggc
     8821 agggcgatgc agcagctcaa caggttcgtg gagctttcaa atgctccgga ctgatgtggc
     8881 acggtccggg gtcatgccgg caggatcgct ttcggatctc caggttcgat ccggcagggt
     8941 cgatatcgag tatacagcat tgacaaggca cattccagcc tggcccggcc cgtcaggagg
     9001 catagatccg gcggaaccgg gattcgatcc ggccggaccg ggatctggcc cgggatggca
     9061 ttggttccgg tatgcatggt aagggaattg atttttaaaa acaatacgtg atccaatgag
     9121 ataagaaaaa cttaacaagg caggtgcaga aacgtgatcc ttgatacgct ggcagcttcc
     9181 gcccggctgc gggtggaagc cgcaaaaatg caaatgccgc tggatgagct aaagcggcag
     9241 gtagcccgga tggatgagat aacagggaga gataagccag gctatctgcc aaaccagaat
     9301 atttcagatc atattgaggc aaaacaacat gccgggcata aagcagcggt ccatattgag
     9361 ccgcagcatg gcgttctcga acccttcgct ttcgaaaaag cattaaaggg aagcggcata
     9421 tcgtttattt gtgaagtaaa gaaggcgtca ccttcaaaag ggatcatagc gcaggatttc
     9481 ccatatgcca ggatcgcatg cgagtatgcg gaagcagggg ccgatgcggt ttctgtcctg
     9541 acggaacccg gatactttct ggggtcggac gcttacctgg aagagatcag caaggctgtc
     9601 gaaattcctg tgctccggaa ggatttcatc gtagacgaat accagatatg gcagtcgaag
     9661 ctcattggcg cggacgccgt actgttgatc tgtgcgttgc tggacactga agcgttggac
     9721 agattcatcg gtatttgcga tgaactgggg ctttccgccc ttgtggaagc ccataccggt
     9781 gatgaggtca gatccgctct ggaagcagga gcgagggtga ttggcgtcaa caaccgggat
     9841 ctcaggactt tcgaggttga tatcaacaat tgcatcaagc tcagggacct ggtgccggaa
     9901 ggagtgattt atgttgcgga aagcggcata aacaccagag aagatatact catgcttgaa
     9961 aaagccggcg tcgatgcggt gctgatcggg gaaaccctta tgaaaagccg cgacaggagg
    10021 tccatgttgc cgtaccttag gggagatact gcagtgaaag gaaaagccgg agggaaagga
    10081 tgatacagca ataagaaggg aaatatgaag tgagaggatg aaaccgcaat aatatgatga
    10141 ggacaccgga catggcgaaa gcgccaggca tgacaaaaat aaagatat
//
LOCUS       DAOSCC010000075        10174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4334_length_10174_cov_3.849491, whole genome shotgun sequence.
ACCESSION   DAOSCC010000075 DAOSCC010000000
VERSION     DAOSCC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_10174_cov_3.849491"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLG72_05520"
     CDS             <1..310
                     /locus_tag="PLG72_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOJ80292.1"
                     /translation="LVSVIQGKGLDYVVDLRPDSKTYKQWKAVELCADNAMAIYIPEG
                     YGHAFLSLEDNTIQQFAIDEYFAADHTKQINYKDPQIGLSLPLDNIILSDYDKNAPYL
                     "
     gene            333..578
                     /locus_tag="PLG72_05525"
     CDS             333..578
                     /locus_tag="PLG72_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80293.1"
                     /translation="MKPAIAPWVIWKALKPAFDEMFRVTRQDGYLLFIATWKMDIAVP
                     DELKNIINDYGNIAMLLEIRNSKYNVHALAIFIYRRG"
     gene            585..2987
                     /locus_tag="PLG72_05530"
     CDS             585..2987
                     /locus_tag="PLG72_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ80294.1"
                     /translation="MTLHEQAKDIVARMTLEEKASLCSGKNFWELKEIERLGLKSHML
                     TDGPHGLRKQEGKADHVGINKSVPAACFPTAAATACSFDPELLYEMGEALGEKCIREN
                     VSVILGPGINIKRNPLCGRNFEYFSEDPYLAGELASALVDGIQSKGVGTSLKHFAANN
                     QETARMVCDSVVDERALREIYLSAFERVVRKSRPWTVMCSYNKLNGTYASENKWLLND
                     VLRDEWGFDGVVVSDWGATAGIVNGIRAGLNLEMPYSGPVNAKRICKAVEKGELAADE
                     LDRCVTSIVELYLKAQQEKKPSARTEEDDHRLARKVAVQSMVLLKNNGILPLNKEDNV
                     AFIGQMAKKPRYQGTGSSRINPSFLDNAYDEAVKAGYRVAYADGYLLDGKDENGDKLK
                     EAVGVAGSSDKVVVFAGLPDEYESEGFDRTSLKMPEEQLKLINAVAKVNKNVIVVLHC
                     GGPVELSWKDDVAAILLAYLSGQAGGSATVDLLWGKENPSGKLAETWPVSLESCPSYK
                     YFPGNLKSVEYRESIYVGYRYYDAVGAEVNYPFGYGLSYTAFDYSNLVIEKMGENEFT
                     VRVDVENTGDCFGREVVQLYVAKPDSAIFRAPRELKGFAKAALDPGEMKTVTFRLNKD
                     SFRYYNVKARSFCIEGGTYLICIGRNSRDMVLEAEVDVIGDGKEELLGDDMKYLTDYA
                     KPKMPFTASDEQFARLLGRRPSSFDPDAERIYDASSTLEDIRDTFIGRMILKKVSKRP
                     GFTEDDTDAATKKMFDAMIMSMPLRALMTMGGTMDLDQVDGIVDMANGHYLRGIRKLL
                     KK"
     gene            3169..4080
                     /locus_tag="PLG72_05535"
     CDS             3169..4080
                     /locus_tag="PLG72_05535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ80295.1"
                     /translation="MKRFILFCEKIQYLGLLGLLGLILDNKQLYLLWLFWLFGLVPVF
                     YNPAVFFQLLKQLLGMIIISLKYGSRIPSAERYIGSVKYSLPFRGAWTVVNGGVEKET
                     SHSWEIQTQRYAYDFLIIDDEGNSFSGSRTNPEDYYCYGKEVLAPADGEVVEVKDKYP
                     DSAILDNGQTDCAARDIRGNYILIRHADREYGLLAHLKHGSIRVKPGDIVKRGQHIAN
                     CGNSGNTSEPHLHFHVQDGRSFYISAGLPILFTDIDSHPAPGYAAIDPRPVPPVSELG
                     KRFIMRGQIVSNRSPAGSNAINASVLV"
     gene            4117..4608
                     /locus_tag="PLG72_05540"
     CDS             4117..4608
                     /locus_tag="PLG72_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80296.1"
                     /translation="MITIKQASADDIPVIEDILLDAVNWLDSTGNSLWTKEQVSWQRL
                     SRDFTAENFYIAYLDGEAAGCMALADYDPVFWPDVQKGESLFLHKLAVKRPAGGKGVS
                     NALIHYAKNECRKRNIKYIRLDCHQFRDKVRKLYESEGFICVDERCLWGKYHTAFYEC
                     RVE"
     gene            5256..6932
                     /locus_tag="PLG72_05545"
     CDS             5256..6932
                     /locus_tag="PLG72_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80297.1"
                     /translation="MKGQWEFYWDRPLDDVQIWDDETPVFVDAPGKWSQYVINGAKLP
                     EKGKATYRIRITGAQTDEVYGVRIKDMRNVYRLYADGILIAQNGNFGDDSSAPASDYR
                     PQLTYFTPKADTFELVMQVSNSTYGNGGMLEPIILGTYAQVLTFDRQLSNAVTYAMTV
                     QLISCLFFLIFFLVQRGEKEALIMSFLAAVILLRLSIIGDTLYTIFPNITAVWLLRFY
                     LLCTPWAEFLLLYLHGLTASEAALYQEHIMQEPMIQGIIACQQENGWIGRGLHGGLDT
                     QEGATKYLAEKAVDRETPVLKLAMEAFARIPIDDWCYDTRGKLIDEFKATGHGHNLIR
                     CACIARAGYDDVIDISPQVRLSLDCFRRVLEVDSVLDITHTVRGGKLLVFNDNERWPC
                     RYHLDILAHTSSWKNEDNIRMIADSVAKLMKTDRPELVNLVPLSWVGHPVGPLGAFPA
                     QGLTVKSTTLLPSPMSIPYRGRPEVYQLEYIEWFARCGIVKYVPALREAVEDIIRYVD
                     DEGICHAPVLELKDWGPYCGFRLETDWRSKTRKACDITFRALLIQHYADL"
     gene            7019..7888
                     /locus_tag="PLG72_05550"
     CDS             7019..7888
                     /locus_tag="PLG72_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ80298.1"
                     /translation="MNEKDMYAADQIKAQQQPVPGKESAMDPEPIYDDPDKKGTGRLM
                     SRTAIITGGDSGIGRAVAVAYAKEGCNVVIVYNIADDDANETKRAAESYGAKVLLIKG
                     DIGDSSFCNEVVDRTLKEFGSIDILVNNAAEQHMQKSLENITDEQLLRTFRTNIFSMF
                     YLTRAALPYMKEGSAIINTSSVTAFKGNRDLIDYSATKGAVTAFTRSLSQNLADRKIR
                     VNQVAPGPIWTPLIVSTLEPNAVENFGKDTPLKRPGQPVELAEAYVYLASSDSSYMTG
                     QTIHINGGTVING"
     gene            7999..>8534
                     /locus_tag="PLG72_05555"
     CDS             7999..>8534
                     /locus_tag="PLG72_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ80299.1"
                     /translation="MSLYDKVSTGLEGFDQVIDHLRFGDNVVWQVDSIPGYKKMAGCF
                     AESAKAENVNLVYIRFADHEPLLDADAGFKTYHIDANKGFEGFATEIHNIISEEGKRV
                     FYIFDCLTDLLEYWHSDLMIGNFFRATCPYLYELDTVAYFAIMRNYHTYSTIAGIRET
                     TQLLLDLYQISDKTYIHPL"
     assembly_gap    8535..8634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8635..>10174
                     /locus_tag="PLG72_05560"
     CDS             <8635..>10174
                     /locus_tag="PLG72_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOJ80300.1"
                     /translation="SIASREIGTGFIGGKSVGMLLARKILEVEGGDRSTPYLEPHDSF
                     YIGSDVFYTYIVQNGWWKLRTMQKTPEGYYKYAAELKEKLLHGTFPKDIQEQFMQMLE
                     YFGQSPIIVRSSSLLEDNFGNAFAGKYESVFCVNQGTPQERYEAFEQAVRTVYASTMN
                     EDALNYRMNRGLAMQDEQMAILVQRVSGDRHGDFFFPHVAGVGNSVNLYVWDESIDMN
                     AGMLRLVFGLGTRAVDRTDGDYVKVVCLDNPLRIPPMDHEDQAKFSQHRLDLLSLSEN
                     ALASRDLDDVLSLPLKTDQNLFISPDHAAAARMRERGYKDMRTPYLLDFKKLFTETKF
                     AAIMREMLALLSKAYDYPVDIEFTANFDKSKGFKINLLQCRPLQTKGLGRTVKIPELK
                     DVKDCFISCKGNFMGGSVRLPVDYVVFVRAGAYLKLSERDKYEVARQIGLINREMKGK
                     NAMLAGPGRWGSTTPSLGVPVHFTELCNMEVLCEYSSKKEGFMPELSYGSHFFQDIVE
                     SEIFY"
BASE COUNT         2781 a         2148 c         2712 g         2433 t
ORIGIN      
        1 gctggtgagc gttatacaag gaaaagggct ggactatgtt gttgatttgc ggccggactc
       61 gaaaacctat aaacagtgga aggcagttga actgtgtgcc gataatgcca tggcaattta
      121 tatacctgag ggatatgggc atgcattctt atctttggaa gacaatacga tccagcaatt
      181 tgcaatagat gaatattttg cggcagatca tacgaaacag atcaactata aagatcctca
      241 gatcgggtta agtttgccgc tggataatat aatactttct gattatgata aaaatgcgcc
      301 ttatttataa aaagcatggt tggagtgttc agatgaagcc tgctatagcg ccctgggtca
      361 tctggaaagc attaaagcct gcttttgatg aaatgtttcg ggtcaccagg caagacggtt
      421 atttgttgtt tattgcaaca tggaagatgg atatagcagt ccccgacgag ttgaaaaata
      481 tcataaatga ttacggcaac atagcaatgc ttttagaaat cagaaacagt aaatacaatg
      541 tgcatgcact ggcgatattt atatatagaa gggggtagcg gaaaatgaca ctgcatgaac
      601 aggcaaaaga tatcgtggca aggatgacgc tggaggaaaa agccagttta tgttcgggaa
      661 aaaatttctg ggaactgaaa gagatagaga ggcttgggct gaaatcgcac atgctgacgg
      721 acggccctca cgggctcagg aaacaggaag ggaaggcaga ccatgttggt ataaataaaa
      781 gtgtccccgc ggcatgtttc cccactgcgg cagctaccgc gtgcagcttt gaccctgaac
      841 ttctttacga gatgggtgag gcattaggcg agaagtgtat ccgcgaaaat gtttccgtca
      901 tactggggcc cggtatcaac ataaaaagga atcccctgtg cgggcgcaac ttcgaatatt
      961 tttcggaaga cccgtacctg gccggagagc ttgcatccgc tcttgtcgac ggcatccaaa
     1021 gcaaaggggt gggaacatcc ctgaaacatt tcgcagctaa caaccaggaa accgcaagga
     1081 tggtatgcga cagtgtcgtt gatgaaaggg cgctcagaga gatctatttg tctgcgtttg
     1141 agagagtagt caggaaatcg cggccatgga ccgttatgtg cagctataac aagctgaacg
     1201 gaacttatgc aagcgagaac aagtggcttt taaatgatgt gctcagggat gaatggggat
     1261 ttgatggagt ggtcgtaagc gactggggcg caacggccgg gatagtgaac gggatcaggg
     1321 cgggcctcaa ccttgagatg ccgtacagcg ggcctgttaa cgcaaagcgc atatgcaaag
     1381 ctgttgaaaa aggggaactg gcggctgatg aactcgacag gtgcgtgacc agcatcgttg
     1441 aattatatct gaaggcccag caggaaaaga aaccttccgc aaggacggag gaagatgatc
     1501 acaggcttgc ccgcaaggtt gccgtacagt ccatggtcct tcttaagaac aacggcatcc
     1561 ttcctctgaa caaagaagac aatgtcgctt ttatcggaca gatggccaaa aagccccgtt
     1621 accagggaac gggaagcagc aggatcaacc cgtccttcct tgacaatgca tatgatgaag
     1681 cggtaaaggc aggatacagg gtggcctatg cagacgggta tctgcttgat gggaaagatg
     1741 aaaacggaga taaactcaaa gaagcagtcg gagttgcagg atcttccgat aaagtagtgg
     1801 tttttgccgg ccttcccgac gaatatgaga gcgagggatt tgaccgcacc tcgcttaaga
     1861 tgccggaaga acagttgaaa cttataaatg cggtcgccaa agtgaataaa aatgtcatag
     1921 tggtgttaca ttgcggaggt ccggtggagc tgtcgtggaa agatgacgtt gccgcgattt
     1981 tgctggcata cctttcaggg caggccggcg gatcagcaac ggtcgatctg ctctggggaa
     2041 aggaaaatcc aagcgggaag ctggctgaaa cctggcccgt ttcactggag tcatgcccgt
     2101 cttacaagta cttcccgggc aatctcaaat cagtcgagta ccgcgaaagc atttatgtcg
     2161 gttatcggta ctatgatgcc gtcggggcgg aagtcaatta tccttttgga tatggcctgt
     2221 cgtatacggc ttttgattac agcaatcttg tgatagagaa aatgggagaa aacgaattca
     2281 ccgtcagggt cgatgtggag aatacaggcg attgctttgg gagggaagtg gtgcagcttt
     2341 atgtagcaaa gccggacagc gccatattcc gcgctccaag ggaattgaaa ggatttgcaa
     2401 aagcagcgct ggatccgggt gaaatgaaga cggttacatt caggctcaat aaagacagtt
     2461 tcaggtatta caatgtgaag gccaggtctt tctgtatcga aggaggaacg taccttatct
     2521 gtatcgggag aaacagcagg gacatggtac tggaagctga agtcgatgtt ataggggacg
     2581 gaaaagagga acttctcggg gacgatatga aataccttac cgattacgct aagccgaaaa
     2641 tgccattcac cgcatcggat gaacagtttg ccaggctgct tggccgcagg ccgtcgagct
     2701 tcgatccgga tgctgaacgg atatatgacg cttcctctac actcgaagac atcagggata
     2761 cttttatcgg caggatgata ttgaagaaag tcagcaaacg tccaggtttt acggaagatg
     2821 atacggacgc cgccacgaag aagatgttcg atgccatgat catgtcgatg cctttgcggg
     2881 ccctgatgac catgggaggc actatggatc tcgaccaggt cgacggcata gtcgatatgg
     2941 caaacgggca ctatctgcgt ggtatccgaa agcttttgaa aaaatgaata cgtataattt
     3001 tattttgcta tgttgtcaat tgatgccagt tagtttacaa ttaaatatta tcagtttttt
     3061 ctgcagaaat aacggcggca tcagatttgt ctgtgtgcct gagggcaggg atatggatta
     3121 tttttagatt aagcgcggcg gagtgtattg cagtggggag aagacatatt gaaacgattt
     3181 atactgtttt gtgaaaaaat acaatacctg ggccttcttg gcttactggg cctgatcctt
     3241 gataacaagc agttgtactt gctgtggctg ttctggctgt ttgggctcgt ccctgttttt
     3301 tataaccccg ctgtcttttt ccaactgctg aaacagctac tggggatgat aatcatttcc
     3361 ttaaagtatg gttcccgtat cccaagtgcg gagcgctaca ttggaagcgt gaagtattct
     3421 ttgccctttc gcggagcatg gacggtagtc aatggaggag ttgaaaaaga aacttcccac
     3481 tcctgggaaa tccagacaca acgctatgct tatgactttc ttatcattga tgatgaaggg
     3541 aacagctttt ccggcagccg cacgaatcct gaagattatt actgctacgg gaaagaggta
     3601 ctggctccgg cggacggtga agtggttgaa gtcaaggata agtatcccga cagtgcaata
     3661 ttggataacg gtcagacaga ttgcgccgcc agggatatcc ggggcaatta tatcctcatt
     3721 cgtcatgcgg acagggaata cggattgctg gctcatctga agcatggcag catccgtgtg
     3781 aagccgggcg atatcgtaaa gcgcggtcag cacatcgcga attgcggcaa ctccggcaac
     3841 acgtcggaac cacaccttca ttttcatgtg caggatggca ggagtttcta catatcagcg
     3901 gggttgccga tcctatttac agatattgat tctcatcctg cgcccgggta tgcagccatc
     3961 gacccccgcc cagtcccgcc ggtttctgaa cttggcaaaa ggtttattat gcggggacaa
     4021 attgtttcaa acagatcacc ggcaggttcg aatgccatca atgcttctgt cctggtttga
     4081 cattatagat ccggctttat attaagtggt ggtgaaatga tcacaataaa acaagcttcc
     4141 gctgatgata tacctgttat agaagatata ttgctcgatg cggtgaactg gctcgacagt
     4201 accggcaatt cgctgtggac aaaggaacag gtgtcatggc agcgtctatc gcgtgatttt
     4261 acagcagaaa acttttacat agcatacctg gacggagaag cagccggctg tatggctctt
     4321 gccgattacg atccggtatt ctggccggat gtgcagaaag gcgaatccct ttttttgcac
     4381 aagctcgccg taaagcggcc ggctggcgga aaaggcgtat cgaacgcttt aatacattat
     4441 gcgaaaaatg aatgcaggaa gagaaatata aagtatatcc ggctggattg ccatcagttc
     4501 cgcgacaaag tcagaaagtt atacgaaagc gaaggattta tctgtgtcga tgaaaggtgc
     4561 ctgtggggga aataccatac ggcattttat gaatgcagag tagagtaaga tgttggcggt
     4621 ttgcccatag atatttatgg atcagggtat atttccgcag atagactata atgtaagata
     4681 tagaaaatgc tatgacttgt taaaagaaaa tggatacttg gttttattgt ggaatgttgc
     4741 cccggagatc aacattccgg aaataaaaaa agcatatgac ctttacgaag tagttgaaac
     4801 ggatttttat actaccgtat acatcgctag aaagaaatga tgttcacagg aacggcagac
     4861 tttgtaatga tatagctgat gaagtttata gacttttttc ttcattccat gaactataag
     4921 ttttcttacg aaagaagtat aatgaaaaaa aggtaagggt gacggataaa ctggaggcgt
     4981 cttgttcagg gggtaggatg aggtcgagaa aagatgaacg tgcaaggtgg attggaagcc
     5041 aaaatacatt aaaccatctg cttcgtcgtc cctctatggc gctgcatgaa ggcatgggtt
     5101 ggaaaaagac ggcaaggatt attctgcctg tttttattgc attgcttatc attgcaggcc
     5161 tttttttacc agcgcgatat gctggcagcc aaatgcccca ggcccggaat ggcgtgcttg
     5221 atcttgccag ttggaatgga aagcaggctt ttgccgtgaa gggccaatgg gagttttatt
     5281 gggaccgtcc tctggacgat gtgcaaatat gggatgacga gactcccgtt ttcgtagatg
     5341 ctcccggaaa atggagccaa tatgtaatca acggtgcaaa actgcctgag aagggaaagg
     5401 cgacctatcg tatccggata acaggagcac aaaccgacga agtatacggt gtacgcatta
     5461 aggatatgag aaatgtctac cgactatatg cagatggcat tctcatagcc cagaatggaa
     5521 atttcggtga tgattcatcg gctccggcat cagattaccg gccacagctt acttacttta
     5581 cccctaaggc agacaccttt gagttggtaa tgcaggtaag caacagtaca tacggaaacg
     5641 gcggcatgct ggaaccgatc attctgggta cctatgcgca ggttttgaca tttgaccggc
     5701 agctctcaaa cgcggtcacc tacgcaatga cggtgcagtt aatatcatgc ctgttctttc
     5761 taatattttt ccttgtacag cgcggcgaaa aagaggcctt gatcatgtct ttccttgcag
     5821 ccgttattct gcttcgcctg tccatcattg gcgacacgtt gtataccata tttccaaata
     5881 tcacagctgt atggctgctc cgtttctatc ttttatgcac accctgggcc gagtttttat
     5941 tgttgtattt gcacggcctc acggccagtg aagctgccct gtaccaggag catatcatgc
     6001 aagaaccgat gatacagggc ataatagctt gtcagcagga gaacggatgg atagggagag
     6061 gtcttcacgg agggcttgat acacaggagg gcgcgacaaa ataccttgca gaaaaagcgg
     6121 tggacaggga aacaccggtg ctcaaactag ccatggaggc tttcgcaagg attcccattg
     6181 atgattggtg ttatgatacg cggggaaaac taatagacga gttcaaggca acagggcacg
     6241 ggcataatct catccgctgc gcctgcattg cccgcgccgg gtatgatgat gtaatagaca
     6301 tatctccaca ggtccggttg tcgctggatt gcttccgccg tgtactcgaa gttgattcgg
     6361 tgctggatat cacccatacg gtccgcggcg gaaaactgct tgttttcaac gacaatgaaa
     6421 gatggccctg ccgctatcat ttggacatac tggcccatac aagctcatgg aaaaatgaag
     6481 ataatatcag gatgattgct gattccgtcg ctaagctgat gaaaaccgac cgcccggaac
     6541 ttgtgaacct ggttccctta tcgtgggtcg gacatcctgt aggcccgctg ggagcgttcc
     6601 cggcacaggg gctgaccgtt aagtcaacca ccctgttgcc gagccccatg tcgattcctt
     6661 accgcggcag gccggaggta tatcaactgg agtatatcga atggtttgcc cgctgcggta
     6721 ttgtaaagta tgttcctgca ttgagggaag cagttgaaga tattatcaga tatgtcgatg
     6781 atgaaggtat ctgtcacgct cccgtgttgg aattgaaaga ctggggacca tactgcggtt
     6841 tccggctgga aacggactgg aggtcaaaaa cccgcaaggc atgcgatatc accttcaggg
     6901 cattattgat acagcattat gccgacttat gagaagtatg gcaactatcc tgcatgattc
     6961 tattttcaac aggaatacta gaaaagagca tcaccaaaaa atgaaagggt agttggccat
     7021 gaacgaaaag gatatgtatg cggcagatca gataaaagct cagcaacaac ctgtaccggg
     7081 aaaagaaagc gcgatggatc ctgaaccgat ctatgatgat cctgataaaa aaggtaccgg
     7141 caggctgatg tccaggaccg ccatcatcac gggcggagac agcgggatcg gcagggctgt
     7201 tgctgtcgcg tatgcgaaag agggctgtaa cgtagttatc gtatataata ttgcagatga
     7261 cgatgcaaat gagacaaaaa gggcagcgga atcttatggc gcaaaggtat tactgatcaa
     7321 aggcgacatc ggggacagca gcttctgcaa cgaggtagtg gacaggaccc tgaaagaatt
     7381 cgggagcata gatatcctgg tgaataatgc cgccgagcag catatgcaaa aatccctgga
     7441 aaatatcacg gacgagcagc ttctacggac gttcaggaca aatatatttt ccatgttcta
     7501 cctgacaaga gccgcgttgc cgtacatgaa agagggcagt gcgattatca ataccagttc
     7561 tgtgacagca ttcaagggaa accgggacct gatcgattat tctgcgacaa aaggagctgt
     7621 aacggcattc acgagatcct tgtcccagaa cctggcggac aggaaaatca gggtgaacca
     7681 ggtagcaccc ggaccgatct ggacgcctct catcgtatct actcttgaac cgaatgccgt
     7741 tgagaacttt ggcaaggata cgccgctgaa aaggccgggg cagccggttg aactcgcgga
     7801 agcctatgtt tatctcgcat cttccgattc ttcatatatg acgggacaga ccatccatat
     7861 aaatggcggt actgttatca acggatgatt gcaggaagat atagaatcat ataagtccgg
     7921 tcctgtggaa ttgctgtgta gatccggccg cccgatgggt ataaatatat agatgttcat
     7981 actaaaacgg ggtgggctat gagtttatac gacaaggtca gcacagggct ggaaggtttc
     8041 gaccaggtga tcgatcatct gcggttcgga gataacgtag tatggcaggt agattccatt
     8101 ccgggctata agaaaatggc cggctgcttt gcagaaagcg cgaaagcaga gaatgtcaac
     8161 ctggtctata tccgctttgc ggatcatgag cccctgctgg atgccgatgc gggtttcaag
     8221 acttatcata tcgacgcgaa taaaggcttt gaaggttttg ccactgagat acacaatatc
     8281 atcagtgaag agggcaaaag ggtattctat atttttgact gcctcaccga tttgcttgaa
     8341 tactggcatt cggacctgat gatcggcaat tttttcaggg cgacctgccc gtacttgtat
     8401 gaactggata ctgtcgcata tttcgcgatc atgcgcaatt accacaccta cagtaccatc
     8461 gcaggcatcc gtgaaacgac gcagcttctc ctggatctgt accagatcag cgataaaacc
     8521 tatatacacc cgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccatt
     8641 gcatcaaggg aaataggcac aggctttatc ggcgggaaga gcgttggtat gctcctggcc
     8701 agaaaaatac tggaggtcga aggcggcgac cgatctacac cgtatttgga accccacgat
     8761 tcgttttata tcggttccga tgttttttac acatatatcg tccagaatgg ctggtggaaa
     8821 ctccgcacca tgcaaaaaac gcccgaaggt tactacaagt atgcggcgga actgaaagaa
     8881 aaacttttgc acgggacatt cccgaaggac atccaggagc agttcatgca aatgctggag
     8941 tatttcggcc agtcgcccat tatcgtacgt tccagttcgc tgctggaaga caactttggg
     9001 aacgcatttg caggcaaata tgagagtgtt ttctgcgtga accagggaac gccccaggag
     9061 cgctacgaag cctttgaaca ggcagtgcgc actgtttatg cgagcactat gaacgaagat
     9121 gcgctgaact accggatgaa ccggggcctg gcaatgcagg atgagcaaat ggccatattg
     9181 gtgcagcgcg tatcaggaga ccggcacgga gactttttct tcccacatgt cgcgggcgtc
     9241 gggaattctg taaaccttta tgtctgggat gaaagcatcg acatgaatgc agggatgctc
     9301 cgccttgtgt tcgggctggg aacccgggcg gtggaccgga cagacgggga ttatgtaaag
     9361 gtagtgtgcc ttgacaaccc tctccggatc cctccgatgg atcatgagga tcaggcgaaa
     9421 ttctcccagc atcggctgga tctcctttcg ctttcggaaa atgccctggc aagcagggat
     9481 ttggatgatg ttttgtccct gccactgaaa acggaccaaa acctgttcat aagccccgac
     9541 catgcggcgg cagcccgtat gcgcgaacgg ggatataagg atatgaggac gccttatctg
     9601 ctggacttca aaaagctttt cacagagacg aagtttgctg ccataatgag agaaatgctg
     9661 gctttgctgt ccaaagcata cgattacccg gtcgatatcg aattcacagc caacttcgac
     9721 aagagcaaag gctttaagat aaacctactg caatgtcgtc cgctgcaaac aaaggggctg
     9781 ggcaggacag taaagatacc ggaacttaag gatgtgaagg actgcttcat atcgtgcaaa
     9841 ggcaatttca tgggaggcag cgtgcgcctt cccgtcgatt atgtcgtatt tgtcagggcg
     9901 ggtgcatatc tgaagctgag tgaaagggac aaatacgagg tagcaaggca gataggcctt
     9961 atcaacaggg aaatgaaagg gaaaaatgca atgctggcgg gcccgggcag atgggggagc
    10021 actacgccgt ccctcggggt cccggtgcat tttaccgaac tatgcaatat ggaagtcctg
    10081 tgcgagtatt cttcgaaaaa agaaggcttc atgccggaac tgtcctatgg cagccatttc
    10141 ttccaggata tcgttgaatc ggagatcttt tatg
//
LOCUS       DAOSCC010000076        10079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4385_length_10079_cov_4.896349, whole genome shotgun sequence.
ACCESSION   DAOSCC010000076 DAOSCC010000000
VERSION     DAOSCC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4385_length_10079_cov_4.896349"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /gene="mraZ"
                     /locus_tag="PLG72_05565"
     CDS             <1..402
                     /gene="mraZ"
                     /locus_tag="PLG72_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ80301.1"
                     /translation="DPKGRIIIPAAFREELDVKFMIAKGLEGCLFIFSMPKWNDLVEK
                     IETLPLSNTKAREFSRFFFSSAAECKVDSHYRILIPPELRVHAGLEKDVTIVGVGNRV
                     EVWSKDRWENYMGGESLSPESLSETFAMLGI"
     gene            445..1383
                     /gene="rsmH"
                     /locus_tag="PLG72_05570"
     CDS             445..1383
                     /gene="rsmH"
                     /locus_tag="PLG72_05570"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ80302.1"
                     /translation="MLYEHKPVMLEECITSLNIDKDGIYIDGTLGGAGHSAEIIKRLD
                     KGTLIGIDQDSNAIKVSEERLSRVRKNAGLVLIKGNFRNMADLVRQRGFDKVDGILLD
                     IGVSSHQLDEAERGFSYQQDAPLDMRMDRDNPLDAATIVNTYDEDEIRDIIREYGEEK
                     WAARIASFIAAARSRNRIETTGQLAEIIKAAIPAKARRNGPHPAKRTFQALRIAVNDE
                     LGALKDAVESAVSLLKPGGRLTIITFHSLEDRIVKTEFQKRENPCVCPPSFPVCVCGR
                     KPELKVLTRKPVLPSKDEVERNPRARSAKMRTAVKL"
     gene            1804..1986
                     /locus_tag="PLG72_05575"
     CDS             1804..1986
                     /locus_tag="PLG72_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80303.1"
                     /translation="MMTGKTQMKWELQGIGLMTGSRNPITVSENEGCKEAVQASWAKD
                     KAYIASEVNTIESEGL"
     gene            1967..2455
                     /locus_tag="PLG72_05580"
     CDS             1967..2455
                     /locus_tag="PLG72_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOJ80304.1"
                     /translation="MNQKGYEYVYGSAARQLEYDVYEENQVLRAKKTYRSHRKIKLRM
                     VMAILAVFAAGLAVVCRYAIITKVSYQINQKQKAYEEIKNENSLLRVQIETKTDLNEI
                     KEVAENRLGMQMPDKSQIVYIKVPRNDYTVLMTSNNSEEKSGNAISKFISRATGLLKF
                     FE"
     gene            2589..4790
                     /locus_tag="PLG72_05585"
     CDS             2589..4790
                     /locus_tag="PLG72_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ80305.1"
                     /translation="MAAPGMSAKKRLMVLLVSFTVIVIGLIVRIAHIQFVEGYELQKK
                     AFIQQNTGRVISPIRGTIYDRNGKKLAISVQAATISCNPNEIAKNKKLTTEQVAEGLA
                     RLLSMDKDSVYSIITKNVSSAIIKRKVDRETEIRIRTWISENSVRGINIDEDAKRYYP
                     GGNLMSHILGFTGDDNQGLMGGIEEVMDKYLKGVPGKILSEVDARGSAVPSEREKRID
                     AQDGLNVVLTIDETIQYLVSKSLDKAIADNKVKGGAVGIVMDPNTGDLLAMVSKPDYD
                     LNSPFAYPENIELDIDPSGWKERSKETVEILSKTVWRNKAVSDTYEPGSTFKSITASA
                     GIEEGVVAPDTITDDFPIKVTGWPKPINCWRVGRPHGTETFREAVYNSCNPAFVKVAQ
                     SLGIDRFYSYVRAFGFMDKTGIELKGEAKSQFQSKPKEIDMAVASFGQRFTITPLQLV
                     TAYTAIVNGGKLMKPRLVKELTDSEGNVVTRFEPEVVRTVISKQTSDIMRDILEGVVA
                     DPMGTGNNAYVRGYRVGGKTGTSETTQDDVYIASFCGFAPADDPKIVVLVALFDPKGD
                     SHMGGVIAAPVVGKIIEDTLSYMQVERRYTEDDMKSMASAVTVPDVRNRSVGEAVKAL
                     KTAGLKYSIEGEGNTESVVVEQMPKPNAVVPEGSVVILYTYKPEEPEIVSMPDLSGKT
                     ISEAAYAMNRVGLNIKVSGNGTVYRQQYEPGAMIEKGTVVEVEFRNMVNVE"
     gene            4920..6374
                     /locus_tag="PLG72_05590"
     CDS             4920..6374
                     /locus_tag="PLG72_05590"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOJ80306.1"
                     /translation="MLLTDLIDGLDILYARGNLNVDIKGIAYDSRNVGKGCLFVCIGG
                     MKTDGHSYTKQALEKGAAAFIAQKPLALETEAPVIITSNTREALAYVSDKYFGHPSGR
                     FNLIGITGTKGKTTTCFMIRSILEAAGRKTGMIGTLGVRIGDRVLHTERTTPESLDLQ
                     QLFAQMADEGVQDVVMEVSSQGLALHRVKYCDFDTAVFTNLSRDHISPSEHASMDEYL
                     EAKTLLFRYCRTGLVNRDCSYADRVIEKAGCGIFTYSINGKTDIRAVNPVYLPGAVRF
                     DMVSPWYNRSFRVNIPGRFTVYNALAAASVSGVAGIPGDAVALGLERVQVPGRAEPVE
                     TGRDFSVIIDYAHTPDSLENILITVKAFTRGNVICVFGCGGDRDRTKRPMMGAVSGQY
                     ADYTVITSDNPRTEDPDAIIRDIEEGIKTTSGKYICITDRREAIRHAIASASSGDVVL
                     LAGKGHETYQIFGDRTIHFDEREILREILDNDFC"
     gene            6386..7759
                     /gene="murF"
                     /locus_tag="PLG72_05595"
     CDS             6386..7759
                     /gene="murF"
                     /locus_tag="PLG72_05595"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOJ80307.1"
                     /translation="MIGLKISEVIGATHGKLLGGDYGLTVAEISTDSRKISPGSLFVA
                     IRGERFDGNDFIADAFENGAAAVLTDRDVVDFPGKTLVKVSDTLKALHDLASYYRNMF
                     DIPFVGITGSVGKTSTKDMVACALGARYNVLKNEGNLNNEIGVPLTIFRLEPGYEAGV
                     IEMGMSAFGEIRALTRIVRPKVGIITNIGVSHIEKLGSRQNILRAKLELLEGLQPDGL
                     LILNGDDVMLSGVKDLLEVRTVSYGLEENADYQAYNIRSKGEKGIDFNIKLDGNEYTV
                     HVPAPGVHNVYNALAALAAGHELQIPAELLIDGIARYSPGKMRLNIIKSDGLTIINDA
                     YNASPQSVKAAIDVLKEIEAERRIAVLGDMLELGEWSMSAHRETGAYVAGSNTDLIIT
                     VGQAAAYIAEGALETGFPGSGTVVLSNNDEAIRYLKDIIRKGDAILVKGSRGMMMEEI
                     VNALTSG"
     gene            7839..8837
                     /gene="mraY"
                     /locus_tag="PLG72_05600"
     CDS             7839..8837
                     /gene="mraY"
                     /locus_tag="PLG72_05600"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ80308.1"
                     /translation="MDLEFILQSTEVAAFAATFIMTVIAGPIIIPLLKRLKFGQTIRD
                     DGPATHLKKMGIPTIGGLIFIIPMTITSLILAPRHPGIIPVLLLILGYAAIGFTDDMI
                     KVVRKNSDGLNPKQKSFGLLLVSAAFAFYAAYHPDIGTGMILPFLGVNAVVELPVFLY
                     IPLIIIVMYLITNSVNMTDGVDGLAAGVTLIVAVFFTIVAMMKPEWDSVKMICAITAG
                     GCLGFLVFNIYPARVIMGDTGSLALGGAVGAAAVLMKMPWMLLVAGAIYAVESLSVFI
                     QVTSYKLRGKRVFKMAPIHHHFELSGWKETKVVGVFWLITIVLCLIGLATLRYDIL"
     gene            8971..10007
                     /gene="ftsW"
                     /locus_tag="PLG72_05605"
                     /pseudo
     CDS             8971..10007
                     /gene="ftsW"
                     /locus_tag="PLG72_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
BASE COUNT         2916 a         2229 c         2796 g         2138 t
ORIGIN      
        1 gatccaaagg gcaggataat cattccggca gctttcagag aagaacttga cgtaaagttc
       61 atgatagcga aaggcctgga aggatgtctt ttcatctttt ccatgcccaa atggaacgat
      121 ctcgttgaaa aaatagaaac actgccactt tcgaacacaa aagcgcggga attcagcagg
      181 tttttcttct caagcgcggc agaatgcaaa gtcgacagcc attatcgcat actgatacct
      241 ccggagcttc gggtgcatgc agggctcgag aaagatgtca ccatcgttgg cgtcgggaac
      301 agagtcgaag tatggagcaa agacaggtgg gaaaactaca tgggtggcga aagcctaagc
      361 ccggaaagcc tgtcggagac gtttgcaatg cttggtatct agtatggggg atctgcggta
      421 cggaaaaacg ccgggaggaa aaagatgctg tatgagcata agcctgtaat gctggaagaa
      481 tgtataacca gcctgaacat agacaaagac gggatatata tcgacggaac gcttggcgga
      541 gccggccatt cggcggaaat aataaaaagg cttgacaagg gcacgctgat cgggatcgac
      601 caggattcca acgccataaa agtatcagaa gaaaggcttt ccagggttcg gaaaaatgcc
      661 ggcctggtac tgatcaaggg aaatttccgc aatatggcgg atctggtcag gcagcgcgga
      721 ttcgacaagg tagacggcat actgctcgac atcggcgtct catcccacca actggatgaa
      781 gccgagcggg ggttcagcta tcagcaggat gcgccgctcg atatgcggat ggacagggat
      841 aatccgcttg acgccgccac gatagtgaac acatatgatg aggacgaaat aagggatatc
      901 atccgggagt atggcgagga gaaatgggcg gccaggatag cttcctttat tgcagcggca
      961 agatcaagga acaggatcga gactacggga cagcttgccg agatcataaa ggccgcgata
     1021 ccggccaaag cgagaagaaa cggcccccat ccggcaaaaa ggacgttcca ggcgctccgg
     1081 atagctgtga acgacgaact cggagcattg aaagatgccg tggaaagcgc ggtatcgctg
     1141 ttgaagccgg gaggaaggct gacaataata acttttcatt cgctggaaga caggatagtg
     1201 aaaaccgagt tccagaaacg tgaaaacccc tgtgtatgcc cgccctcatt cccggtgtgc
     1261 gtatgcggga gaaaaccgga actgaaggta ctgaccagga aacccgtact cccttcgaaa
     1321 gatgaagtgg agcggaatcc aagggccaga agcgcaaaga tgagaactgc agtaaagctg
     1381 tgattcaagt tttacgcaaa agacaaatcg atcgatacac gcatgacaat tgccatatat
     1441 accagggaga aattgcggtg ttgttgtcca cagccggctg caatgatata caaaagataa
     1501 aaccttgata atgcaaaaga accggacaat acgaatgaaa acgccagagc gaccatccgc
     1561 tgcggaagcg gctgaaataa cgacgaacaa taatggctga ccggcatact gaagacaaga
     1621 cccttacata caaaggaaga atcctgatac acaaaggaaa accctgaaat gcaaaggaag
     1681 aaccctgcaa aacaaaagac aaagcaaata aacgcgaatg atggcctgac ggactgacaa
     1741 gagaaaaagc acaagttccg gaagatcatg caccggatct gcgggacgac tgccggaaaa
     1801 cgaatgatga ccgggaaaac acaaatgaaa tgggagctgc aggggatcgg tctcatgacc
     1861 ggttcccgaa acccaataac cgtctcggaa aacgaaggat gcaaggaagc agttcaggca
     1921 tcatgggcaa aagacaaggc atatattgca tcagaggtga atacaattga atcagaaggg
     1981 ttatgaatac gtgtacggtt ctgccgccag acagcttgaa tatgatgtct acgaagagaa
     2041 ccaggtgctc agggcgaaaa aaacatacag aagccataga aaaatcaagc tcaggatggt
     2101 catggcgatc cttgcggtgt tcgccgccgg tctcgctgtg gtgtgcaggt atgccataat
     2161 aacaaaggtc agttatcaga taaaccagaa gcaaaaagct tatgaagaga taaagaacga
     2221 aaactcgctg cttagagtcc agatagaaac gaaaaccgat ctgaacgaaa taaaggaagt
     2281 ggctgaaaac aggctcggga tgcaaatgcc ggacaaaagc caaatagtat acataaaagt
     2341 tcccagaaat gattacaccg tactgatgac atcgaataac agtgaagaaa agagcgggaa
     2401 cgccatatca aaatttatca gcagagcaac cggacttctg aaatttttcg aatagcaggc
     2461 cgggcatccg gatagaggtt gacgtgcccg gcggtcacaa gtgagcatag actgtggata
     2521 gtgtaaaatt tatttttgca cggtctatag tcttttttat ttacgatgta tttgaaggag
     2581 aggtttagat ggcggcaccg ggaatgtcgg caaaaaagcg tctgatggtc ctgctcgtat
     2641 cctttacggt cattgtcatt ggccttattg taaggattgc tcatatacag ttcgtagagg
     2701 gatatgaact tcagaaaaaa gctttcatac agcaaaatac cggcagggtc ataagtccta
     2761 tcagaggcac catatatgac aggaacggca aaaagctggc cataagtgta caggctgcca
     2821 ccattagctg caaccccaat gagatcgcaa aaaataaaaa actgacgaca gaacaggtag
     2881 ctgaaggcct ggcgcggtta ttatccatgg acaaagatag tgtatacagc ataatcacga
     2941 aaaatgtcag cagcgcgatc attaagagaa aggtcgacag ggagaccgag atacggataa
     3001 ggacatggat aagtgaaaac agcgtcagag ggataaatat cgacgaggat gcaaaaaggt
     3061 attaccccgg gggcaacctg atgtcgcaca tactcggatt tacaggggat gacaaccagg
     3121 gtctgatggg cggtatcgaa gaggtaatgg acaaatacct gaaaggcgtg cctggtaaaa
     3181 tactcagcga agtagacgca agaggaagtg cggtgccgtc tgaacgggaa aaacgtatag
     3241 acgcccagga cggcctgaat gtcgtactca caatagatga aacgatacag tatctcgtct
     3301 cgaaatcact tgacaaggca attgcagaca acaaggtaaa aggcggagcc gtcggcatcg
     3361 tcatggatcc caacacgggc gatctgctgg caatggtatc aaagcctgat tatgatctca
     3421 acagcccatt cgcgtaccct gaaaatatcg aactggatat cgatccctcg ggatggaagg
     3481 aacggagcaa agaaaccgta gaaatactaa gcaaaacagt ctggagaaac aaagccgttt
     3541 ccgacacata tgaacccggt tccacgttca aaagcatcac cgcgtctgcc ggcatagagg
     3601 agggtgtcgt agcgcccgat accataaccg acgactttcc gatcaaggtc acaggctggc
     3661 cgaagcccat aaactgctgg cgtgtcggaa gaccgcacgg aacggaaaca ttccgcgaag
     3721 ccgtttacaa ttcgtgcaac cctgcatttg tgaaagtagc gcagagcctt ggcatagacc
     3781 gtttctacag ttatgtgcgg gcattcggat tcatggacaa aacggggatc gaactgaagg
     3841 gcgaagccaa atcccaattc cagtccaagc caaaggaaat cgatatggct gtagcttcat
     3901 tcggacagag gttcacaata acgccgctcc aactggtgac tgcatatacc gctatcgtga
     3961 acggcggcaa actgatgaaa ccgcgccttg tgaaggaact gaccgattcg gaaggcaatg
     4021 tcgtcaccag gttcgagcct gaagtggtac ggacagtcat atcgaaacag acgtctgata
     4081 tcatgagaga catacttgaa ggagtcgtcg cagatcccat ggggacagga aacaatgcct
     4141 atgtcagagg ctacagggta ggaggcaaga ccggcacgtc ggaaaccacc caggatgacg
     4201 tttacatagc atcattctgc ggatttgctc cggcagacga tccgaagata gtcgtactgg
     4261 tcgctttatt cgatccgaag ggagactctc acatgggcgg agtcatagct gcgccggtag
     4321 taggcaagat cattgaagat actctcagtt acatgcaggt agaaaggaga tatacggaag
     4381 atgacatgaa atccatggca tctgccgtga ctgtccctga tgtcaggaac agatccgtgg
     4441 gagaagcggt aaaggcgctc aagacagccg gtctgaaata tagcatcgaa ggtgagggca
     4501 atacagaaag cgtcgttgtg gaacagatgc cgaaaccgaa cgccgttgtc ccggagggct
     4561 cggtagtgat cctgtataca tataagccgg aagaaccgga gatagtatcg atgcccgatc
     4621 tgtcaggcaa aaccatatcg gaggcggctt acgccatgaa ccgtgtgggc ctgaacataa
     4681 aagtgtcggg caatggcaca gtctacaggc agcaatatga accgggcgcc atgatcgaga
     4741 agggtactgt tgttgaagtg gaattcagga atatggtcaa tgtcgaataa tatacatcgg
     4801 atcgataaca tgaaaaacaa gggggaaagg cggcagccgg aaatgcaggg tgtcaggcgg
     4861 ctgccggaaa tggagatgat cgttatgtag gtcaatctgg tagtaaagga gacaaatcca
     4921 tgctgttgac tgatctgatc gatggattgg atatccttta cgcaagagga aatcttaatg
     4981 ttgatataaa agggatcgct tatgattcca ggaacgtcgg gaaaggctgt ctgttcgtct
     5041 gcatcggcgg aatgaaaacc gacggccact catacacgaa gcaggccctg gaaaaagggg
     5101 ctgcggcgtt catcgcgcaa aagccgctgg cactggaaac agaagcgccc gttataataa
     5161 caagcaacac aagagaagcg ctggcatacg tatcggacaa atatttcgga cacccgtcgg
     5221 gcaggttcaa cctgatcggt attacgggga caaaggggaa aacgactacc tgtttcatga
     5281 tcagatccat acttgaggcg gctgggagaa agacaggcat gataggcacc ctgggcgtga
     5341 ggatcggcga ccgggtactg catacggaga ggaccacgcc agaatcgctg gacctgcagc
     5401 agctttttgc acaaatggct gacgaaggag tccaggatgt cgtgatggag gtatcatcgc
     5461 aggggctcgc ccttcacagg gtgaaatact gtgactttga cacggcagtg ttcacaaacc
     5521 tgtcacggga ccatataagc ccgtctgaac atgcaagcat ggatgaatac cttgaggcga
     5581 agaccctttt gttcaggtac tgcaggactg gacttgttaa cagggactgc agttatgcgg
     5641 atagggtgat cgaaaaggct ggatgcggga tatttacata cagcataaac ggcaaaacgg
     5701 acatccgggc ggtgaatccg gtttacctgc ccggggctgt caggtttgac atggtctctc
     5761 catggtacaa cagaagcttc agagtgaata tacccggcag attcaccgtc tataatgcgc
     5821 ttgccgcagc aagcgtgagc ggcgtggcag gcatccccgg ggatgctgtc gcacttgggc
     5881 tcgaaagggt gcaggtgccg ggcagggccg aacctgtgga gacgggacgt gactttagtg
     5941 ttataataga ttatgcacat acgcctgaca gccttgaaaa tatacttatt acagtaaaag
     6001 cttttaccag gggcaatgtt atatgcgttt tcggctgcgg cggtgaccgg gacaggacaa
     6061 aaaggccgat gatgggcgca gtatcaggac agtatgcgga ttatacggtg atcacatcgg
     6121 acaatccaag gactgaagat cctgatgcca tcataaggga tatcgaagag ggtataaaga
     6181 ctacgtcggg aaaatatata tgcataacgg accggaggga agccataaga cacgccattg
     6241 cttccgcatc atcaggagac gtcgtattgc ttgcaggtaa agggcatgaa acataccaga
     6301 tattcggaga caggacgata cattttgatg aaagggaaat cttgagggag atacttgata
     6361 acgacttctg ttaaaaggag catgtatgat cggtttgaag ataagtgaag tgataggagc
     6421 gacacacgga aaactcctgg gaggggacta cggcctcact gtcgcggaaa tatcgacaga
     6481 ttcgcgtaag ataagcccgg gaagtctttt cgttgcgatc aggggcgaga ggttcgacgg
     6541 gaatgacttt atcgccgacg ctttcgaaaa tggagccgct gcagtcctga ccgacaggga
     6601 tgtggtggat tttcccggta aaacgcttgt caaggtaagc gacacgctta aagcgctcca
     6661 tgatctcgct tcgtactaca gaaacatgtt tgacatcccg ttcgtgggaa taacgggaag
     6721 cgtcgggaaa acgagcacaa aggatatggt tgcgtgtgca ttgggtgcgc ggtacaatgt
     6781 gttgaaaaat gaagggaacc tcaataacga gatcggcgtg ccgctgacga tattccggct
     6841 cgagccgggt tacgaagccg gcgttataga aatgggtatg agcgccttcg gtgagatcag
     6901 ggcactgaca cgtatcgtca ggcctaaagt gggaataata accaatattg gtgtttctca
     6961 tatagaaaaa ctcggctcca ggcaaaatat cctgagagcg aaactggaac tgctcgaagg
     7021 ccttcagcct gacgggctgc tgatactgaa cggcgacgac gtcatgctca gcggcgtgaa
     7081 ggatctgctg gaagtaagga cggtatcgta cggcctggag gagaatgccg attatcaggc
     7141 gtataacatc aggtcgaaag gggaaaaggg catcgacttc aacataaagc tggacgggaa
     7201 tgagtatacc gtacatgtac ctgcacccgg tgtacataac gtttacaacg cgctggcggc
     7261 gctggcagca ggacatgaac tgcagatccc ggcagagttg ctgatagacg gcatagcacg
     7321 atacagcccc ggaaaaatgc gcctcaacat catcaagtcc gacgggctga ccatcataaa
     7381 tgatgcctac aacgcgagcc cgcagtctgt aaaggcggct atcgatgtgc tcaaagagat
     7441 cgaagcggaa agaaggatag ccgttttggg cgatatgctt gaacttggcg aatggagtat
     7501 gagcgcgcac agggagacgg gcgcatatgt ggcaggcagc aatacggacc tgataatcac
     7561 agtagggcag gctgccgcat atatagctga aggggcttta gaaacaggtt ttcccgggtc
     7621 cgggacggtc gtattgtcaa ataacgacga agccatcagg tatctgaagg acatcatccg
     7681 gaagggagac gccatactgg tcaaaggatc cagggggatg atgatggaag agatagtcaa
     7741 cgcgctgaca tccggatgat acgccgacat ggaggcccgg aggcacagca gcctatggaa
     7801 caacaaagcg ataaaacaca cattcgaaat acgggggaat ggatttggag ttcatattac
     7861 agtcgacaga agtggcagcc tttgccgcaa cattcataat gacggtcatc gcgggaccga
     7921 ttataatacc gcttctgaag cgccttaagt tcggccagac gatcagggac gacggtccgg
     7981 cgactcattt gaaaaagatg gggataccta ccatcggagg cctcatcttc ataataccta
     8041 tgaccatcac atcattgata cttgctccca ggcatccggg aataattccc gtattgctct
     8101 tgatattggg atatgcagcg ataggcttta cagacgatat gataaaagtc gtcaggaaga
     8161 actcggacgg actgaacccg aagcagaagt cattcggtct gctcctggta tcggccgctt
     8221 ttgcgttcta tgccgcatac catcctgata tcgggaccgg gatgatactg cctttccttg
     8281 gtgtcaatgc ggtggtggaa cttcctgtct ttctgtacat accgcttatc atcatcgtca
     8341 tgtatctcat cacgaattcg gtgaatatga ccgacggcgt cgatgggctt gcggcaggag
     8401 tgacactgat cgttgcagtg tttttcacga tagtcgcgat gatgaagccg gaatgggaca
     8461 gtgtgaaaat gatatgcgcc ataacggccg gtgggtgtct cggcttcctg gtattcaaca
     8521 tatatccggc aagagtcatt atgggcgaca cgggttcgct cgcccttggc ggagctgtgg
     8581 gagccgccgc ggtattgatg aaaatgccgt ggatgctgtt ggtagcaggt gcaatatacg
     8641 ccgtggagag cctttctgtt ttcatccagg tcacatcata caaactgagg ggcaagagag
     8701 tgttcaaaat ggctccgata catcaccatt tcgaactctc gggatggaag gagacaaagg
     8761 tggtcggcgt tttctggctg ataaccatag tactttgcct tatcgggctt gccacactta
     8821 ggtacgatat attatagagc tgcggcacag acggggggaa tgggctgccg gctccgacgc
     8881 gccatatccg ggaggatatt tatgaaaaag aaaccgtttg attttatcct gttcatgaca
     8941 gtactgatac tgctttcgct gggacttatc atggtcttca gcgcaagtgc ccccatggct
     9001 gaaaaagagt cggggaatat atactactat ataaacaggc agttggtctt cgccgccatc
     9061 ggcacggtct ccatgattct tgcagccaat tacgattacc ataagtttgg gaggaaaaca
     9121 gtatgggctc tgtttttggt cagcgttgtc cttctcatac tcgtactgat cccgggtatc
     9181 ggcagtgaag ccaacgattc caggagatgg gtcgtgatag ccggtttccg gttccagccg
     9241 tccgagctgg ccaaattgtc gctcatattg ttcttttcat tcaacctgtc caggaggaag
     9301 aagccgctca actccttttt cggtgacctg atgccgtacc tttttgcgct tggcataata
     9361 tgtgtgcttc ttctgctgga gacgcatctg agtgcaacta taatcatgat cgccatatcc
     9421 atgataatac tgttcgtggc aggcgcgaag atcaggcatt ttgccatatt gatcgtgcct
     9481 gtcgtcgtaa cgataatagc cgtcatttca ttacggatta cacgactccc cgtatcaatt
     9541 catggctgga tccgtggagc gatccccgcg gcagcggtta tcagaccatt cagtctctgt
     9601 atgccatcgc ctccggcggg ctgtttggaa gaggcctggg gcagagcatg cagaaattcc
     9661 tgtatgtacc tgaaccgcac aatgactaca tttttccgat tctagccgaa gaacttggtt
     9721 ttatcggtgt gcttgtggtt atgttgctgt tccttatatt catctggagg gggatcaaga
     9781 tcgcagtcca cgcaccagat gtgtatggat gcctgatagc ttcaggcatt accgcgctga
     9841 tcgccgtcca gagccttttc aatgtggcag ttgtcaccaa ctccgtaccg cccaccggcg
     9901 tatcattgcc atttttcagt gccggcggca cagcccttat tctgttttta acagaagcgg
     9961 ggatactgct caacatctcg cgatactcca catacgaaag aatctgaaca tatcgtaaat
    10021 tttgcgcata agatgaaatg tgtgcgaggt gtttttatga gcaagttggt tatttcagg
//
LOCUS       DAOSCC010000077        10002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4430_length_10002_cov_4.086358, whole genome shotgun sequence.
ACCESSION   DAOSCC010000077 DAOSCC010000000
VERSION     DAOSCC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_10002_cov_4.086358"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PLG72_05610"
     CDS             complement(<1..1186)
                     /locus_tag="PLG72_05610"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOJ80309.1"
                     /translation="MAKVKITETVLRDAHQSLIATRMSIDDILPVVEKLDDIGYHSLE
                     CWGGATFDSCLRFLNEDPWDRLRKIRSKAKKTKLQMLLRGQNLLGYKHYADDVVEYFV
                     QKSIANGIDIIRIFDALNDARNLETAIKACKKEGGHAQAAISYTISPVHNLDMFVKDA
                     RMLEEMGADSICIKDMAGLLLPYAAYDLVKALKENVKIPIQLHTHYTSGVASMTYLKA
                     IEAGVDVVDCAISPFALGTSQPPTEPLVATLKGTEYDTGLDLGKLSDIADYFRPIKEK
                     YIKSGLLDIKVMGVDVNTLKYQVPGGMLSNLVSQLKQAGKADLFEEVLKEVPRVREDF
                     GYPPLVTPTSQIVGTQAVMNVITGERYKMVPKESKALVKGEYGKTPAPIPEHIKKKIL
                     GDE"
     gene            complement(1207..1620)
                     /locus_tag="PLG72_05615"
     CDS             complement(1207..1620)
                     /locus_tag="PLG72_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ80310.1"
                     /translation="MKKFLIKVNGNQYEVEVEEVKSGSAPAYTAAPAQPAAEPAPAPA
                     QAAPAPAPAPAPAAKAAPSGAAGSVKVTAPMPGTILNIVASVGDKVKRGQVLLILEAM
                     KMENEIVAPSDGTVVSINVTSGTSVNAGDLLVSID"
     gene            complement(1662..1850)
                     /locus_tag="PLG72_05620"
     CDS             complement(1662..1850)
                     /locus_tag="PLG72_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOJ80311.1"
                     /translation="MTENENVIIDEEILAVISAAIAAMETRPGYRLVVRSMRQIPQTS
                     PVWNTTGRLERLSRNLNA"
     gene            complement(1866..3416)
                     /locus_tag="PLG72_05625"
     CDS             complement(1866..3416)
                     /locus_tag="PLG72_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ80312.1"
                     /translation="MGTMEKINELRIRRQALEEGGGAERIAKQHESGKLTARERLEKL
                     FDENSFVEVDAFVETRAIDFDMQRKKVPGDGVVTGYGTIEGRPVFAASQDFTVIGGSL
                     GEAHARKIAKVMDMAMKVGAPFISINDSGGARIEEGIDALSGYGEIFFRNTLASGVIP
                     QISVIMGPCAGGAVYSPAITDFVFMVDKTSYMFITGPQVIKSVTGEDVTFETLGGSEV
                     HNSKSGVAHFRCANEEECIKEIRRLISFLPDNNLTDPEVLAETDDPNRLDDSLLEIVP
                     DSSNKPYDMKEIITKVLDNGDFMEVQKHFAQNIIVGFGRLNGKTVGIVANQPNVLAGA
                     LDVDSSDKAARFVRFCDAFNIPLVSFTDTAGYLPGTGQEYSGIIRHGAKLLYAFSEAT
                     VPKVNVIVRKAYGGAYIAMNSKHLGADMVFAWPSAEIAVMGPEGAANIIFRKEIAAAE
                     DAVAYREQKIEEYRSKFSNPYVAAARGYVDDVIDPATTRPRLISALEMLAGKRENRPA
                     KKHGNIPL"
     gene            complement(3748..4467)
                     /locus_tag="PLG72_05630"
     CDS             complement(3748..4467)
                     /locus_tag="PLG72_05630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ80313.1"
                     /translation="MIRLQKYLADAGVSSRRKCEELILQGRVQVNGKTVTEPGTKVEG
                     SEEVRVDGRLIRPGHKKVYILLNKPTGYISAVKDQFSRKTVIDLVSGISERIYPVGRL
                     DYDTSGLIILTNDGEFANMLMHPRNEVEKVYRAEIKGMLSADDIERLENGLDIGGHIT
                     APACVRIIDKSAGRSVVEITIHEGKNRQVRRMFETIGHPVLRLKRTAIGGITAGDLGE
                     GKWRYLSKAEIEMLRSRCGDF"
     gene            complement(4664..5119)
                     /gene="ytfJ"
                     /locus_tag="PLG72_05635"
     CDS             complement(4664..5119)
                     /gene="ytfJ"
                     /locus_tag="PLG72_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOJ80314.1"
                     /translation="MAEQHPIEALMKTAMESIREMVDVNTIVGDAVQTPDGTVIIPIS
                     KVTFGFAAGGGEYGAAEDENDESKTDDEDGSSGIEGKFPFAGGSGAGVSISPVAFMVV
                     GNGHIKLLPVNINSTIDKVLDLVPEFMCRLENICKKKIAVKKEKEEDPE"
     gene            complement(5112..5777)
                     /locus_tag="PLG72_05640"
     CDS             complement(5112..5777)
                     /locus_tag="PLG72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOJ80315.1"
                     /translation="MLYIAVFFILALYTVLFVVRIKAAVEYTRDEQDEWVIFCFYTGK
                     GIIRYEFEVPLAKNDNGKTKFKLVKGQSHKMQGGSKANEKLGPEDIIEKFISVRQYLK
                     DHKSLLEDIREYINRHGIHVEIEVKLRQGTGDAAQTGLVCGALWSAAGIIVAYLSRHL
                     KTISKRIKITPCFNEKVFEVEARCIFHVRLVHIIVVLIKIYWMKHSIVKNSKKATGGG
                     ASG"
     gene            complement(5941..6510)
                     /gene="scpB"
                     /locus_tag="PLG72_05645"
     CDS             complement(5941..6510)
                     /gene="scpB"
                     /locus_tag="PLG72_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ80316.1"
                     /translation="MDIKEMEAIVEGVLFAAGDPVPLAKIAEILELDKATAKRILDNM
                     AIRIQNSQRGILIRELNGSYQLCTRPEHFEYISKLAEPRQKQALSQAAFETLAIIAYN
                     QPVTRAKVEMIRGVNSDSSIATLLERNLIKEAGRLDSPGRPVLYETTEEFLRSFGFRS
                     IKDLPMLELDRMISENQPEEDMQKNDTDT"
     gene            complement(6566..7360)
                     /locus_tag="PLG72_05650"
     CDS             complement(6566..7360)
                     /locus_tag="PLG72_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ80317.1"
                     /translation="MESALTNACTIKIDNFEGPFDLLFHLIEKNQFNIYDIPINTITD
                     QYMDYLFTMQEMDLEIASEFLVMASTLLHIKSKMLLPSRKEEQQQEEDPREELVSRLL
                     EYRKYKDFSYVLREREKEWAKVWWKLPEPITCVRKDEVVELVPEELRRVYTELLERNM
                     KKMNPNVNGMNRIIQHEKVSLRSKMREIIRELIKRGRFRFFELFSFKTRSRADVVTGF
                     LAILELARLRRVRLEQRKIHGEIYVSKVEMAPELGKIDARELDSMA"
     gene            complement(7474..8481)
                     /gene="trpS"
                     /locus_tag="PLG72_05655"
     CDS             complement(7474..8481)
                     /gene="trpS"
                     /locus_tag="PLG72_05655"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ80318.1"
                     /translation="MRANTGRKIMLSGARPTGALHLGNYFGALENWVKLQDEYDCYYF
                     IADWHALTTGYEDTSELGSNITNMVIDWLSVGLDPEKSTLFLQSSVREHAELHLLFSM
                     ITPLSWLYRCPTYKDQIQQLKDKNILTYGFLGYPCLQAADILMYKASAVPVGEDQVPH
                     VELTREIARRFNYLYGEVFPEPQSILTKAKVLPGTDGRKMSKSYNNTIALSDDPETVR
                     KKVMSMVTDPARIRKDDPGHPEVCTVFAFNKVFDEDGVSETEAQCRAGCIGCVQCKKN
                     LYSKMAEKLTPIYEKRQEILSKPDYMGYIKGVIEDGNARARKTAEKTIADVRKAMKID
                     W"
     gene            complement(8561..9286)
                     /locus_tag="PLG72_05660"
     CDS             complement(8561..9286)
                     /locus_tag="PLG72_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ80319.1"
                     /translation="MDRIAMILYAIPAILFSLSFHEMAHAYISYRLGDPTAKRLGRLT
                     LNPLKHLDPLGTLMLVVSMYSGFGFGWAKPVPIDPTYYRNYKKGTVMVSLAGPASNLL
                     LAFIFSFPMAAIASKSGLPYNVIYDSRYMPYLIAFYGQNFSLVSAVFIISGIFYMINI
                     GLAIFNLLPIPPLDGSKILMGVLPARIYFGLMRYERYIGLLFLALMLLRPGILSTVTY
                     PFRRAVELLFGLAGSLIIRYIIT"
     gene            complement(9751..9945)
                     /locus_tag="PLG72_05665"
     CDS             complement(9751..9945)
                     /locus_tag="PLG72_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80320.1"
                     /translation="MNISSDIRAPVKSGQKIGEVIYTLSGKEVGRADIVASSNVQKAT
                     FLRIMLRLMQEWFCLGRAAS"
BASE COUNT         2181 a         2692 c         2112 g         3017 t
ORIGIN      
        1 cctcgtctcc aagtatcttc ttttttatat gttccggaat aggagcaggt gtctttccat
       61 actcgccctt cacgagagct ttggattcct tcggcaccat cttgtacctt tccccggtga
      121 tgacgttcat gacagcctgg gttccgacta tctggctggt aggcgtgacc agcggcggat
      181 atccgaaatc ttcccttacc ctcggcactt ccttgaggac ttcttcgaaa aggtctgcct
      241 tcccggcctg tttcagctgt gaaacgaggt tcgacagcat tccgccgggc acctggtatt
      301 tcagtgtatt gacatcgacg cccatgactt ttatgtcaag cagccctgac ttgatgtatt
      361 tttccttgat aggcctgaag taatcggcga tgtcgctgag tttgccaagg tccagtccgg
      421 tgtcgtattc ggtgcccttg agtgttgcga ccagcggctc tgtgggcggc tgtgacgtac
      481 ccagtgcaaa cggggaaatg gcgcagtcga ctacgtcgac tcccgcttct atagccttga
      541 ggtatgtcat ggaagctaca ccgctggtat aatgagtatg gagctgtatc ggaatcttta
      601 cattttcctt cagcgctttg accagatcat acgcggcata cggcagaagc aaacctgcca
      661 tgtccttgat gcatatgctg tcggccccca tttcctccag catgcgggcg tccttcacaa
      721 acatgtcaag gttatggaca gggcttatag tataacttat agccgcctgt gcatgcccgc
      781 cttctttctt gcaagccttg atcgcagtct ccaggttcct tgcgtcgttc agtgcatcga
      841 aaatccttat tatatcgatg ccgttcgcta tggatttctg tacaaagtat tcgaccacat
      901 cgtccgcata atgcttatat ccgaggaggt tctgccctct caggagcatc tgcagttttg
      961 tcttttttgc ctttgacctg atttttctga gcctgtccca cggatcctcg ttaaggaagc
     1021 gaagacatga atcgaaggta gctcctcccc agcattcaag cgaatgatat ccaatatcat
     1081 ccaatttctc cactaccggc aggatatcat cgattgacat ccttgtggct atcaaagact
     1141 gatgcgcatc tcgtagtacc gtttctgtta tttttacctt tgccatttct ggtccccttt
     1201 catatcttag tctattgaaa caagcaggtc gcctgcatta accgatgttc cgctggtgac
     1261 atttatggaa acaacagtgc cgtccgaagg ggcaactatc tcgttttcca tcttcatagc
     1321 ctcgagtatc agcagtacct ggcctctctt tactttatca ccgacagaag cgacaatgtt
     1381 gagtatcgtt cccggcattg gagccgtaac cttcacagat cccgcagctc ctgacggcgc
     1441 tgctttggct gctggtgcag gagcgggcgc aggtgcaggc gctgcctgtg ctggcgcggg
     1501 agcaggctct gccgctggct gtgcaggtgc tgctgtgtat gccggtgcag agccgctttt
     1561 tacttcttct acttcaacct cgtactggtt gccgtttacc tttatcaaaa atttcttcat
     1621 aatttccatc ctccctgatc agatttgatg tattgtcagt tttatgcatt cagattgcgg
     1681 ctgaggcgtt ccagtctgcc ggtggtattc cagaccggcg atgtctgcgg gatctgcctc
     1741 attgatctta cgaccagcct gtagccgggc ctggtctcca ttgctgcaat ggcagcggat
     1801 attacagcaa gtatttcttc atcgatgatc acgttttcgt tttccgtcat gcctgcacct
     1861 ccgtatcaca gcggtatgtt gccatgcttc tttgccggtc tgttttccct cttcccggcc
     1921 agcatctcca gggcgctgat cagtctcggc ctcgttgtcg ccggatctat gacatcgtcc
     1981 acgtatcccc ttgctgccgc tacataagga tttgagaact tgctcctgta ctcctctatt
     2041 ttttgctcac ggtatgcgac agcgtcttca gccgctgcta tttcctttcg gaatatgata
     2101 ttcgcagcgc cttcgggacc catgaccgct atttcagcgc tcggccacgc aaataccatg
     2161 tctgctccaa gatgcttgct gttcattgct atataggctc caccgtatgc tttcctgact
     2221 ataacattta ccttcggcac tgtcgcttct gaaaatgcat acaggagttt tgcgccatgc
     2281 cttatgatac cgctgtattc ctgtccggtc ccgggaagat atcctgctgt gtcggtgaac
     2341 gataccagcg ggatgttgaa cgcatcacag aaacgtacga atcttgccgc cttatcagat
     2401 gaatccacgt caagggcgcc ggccagcaca ttcggctggt ttgcaacgat gccgacggtt
     2461 tttccgttga gcctgccgaa acctactatg atgttctgtg cgaaatgttt ctggacttcc
     2521 atgaaatccc cgttgtccag aaccttcgtt attatctcct tcatatcgta aggcttgttg
     2581 gaactgtcgg gcactatctc aaggaggctg tcatccaacc tgttgggatc atccgtctct
     2641 gccagcactt ccggatcggt caggttgttg tccggaagga aactgataag ccttcttatt
     2701 tcttttatac actcttcctc gttggcacac ctgaaatgtg cgacaccgct ttttgaatta
     2761 tgtacctccg aacctcccaa tgtctcgaag gtaacatctt cgccggtaac ggatttgatc
     2821 acctgcggcc cggtgatgaa catgtagctt gtcttgtcca ccataaacac aaagtctgtt
     2881 atggccggcg aatataccgc acctccggca cagggcccca ttatgaccga tatctgcggt
     2941 atcacgcctg atgcaagcgt gttcctgaag aatatctcac catatccgct caaggcatct
     3001 ataccttcct ctatccttgc tccgccggag tcattgatac tgatgaaggg cgctccaact
     3061 ttcatggcca tgtccattac ttttgctatc ttgcgggcat gagcttcacc aagcgagcct
     3121 cctattactg tgaaatcctg tgatgccgcg aacacaggcc tcccttcgat cgttccgtag
     3181 cctgtcacca caccgtcccc ggggaccttc tttctctgca tatcgaaatc gattgcccgg
     3241 gtttcgacga acgcatcaac ttcaacgaaa gagttttcat caaacaactt ctcaagacgc
     3301 tcgcgcgctg tcagtttgcc tgattcatgc tgctttgcga tcctctcggc gccgcctccc
     3361 tcttcgagtg cttgcctgcg gatccggagt tcgtttattt tttccatcgt acccatatgg
     3421 ctaccacctc gtcacacaaa tatctgcagg aattatcatt tggtgttatt acctggtgcc
     3481 aatatatttg gattatataa tattttccat tccctttcaa cctgaatatt aacatttacc
     3541 aagccggcaa ataaaaaaaa cctcatccct gtcagtatca actgaacaag ccaaaggtcc
     3601 tcccgtcact tttgaacata aaatcaagcg gtttaaccgc cggcttatga ctgcacaact
     3661 ttggtctgat aacctccaat cattatcaag tgtaccatta caggaacata acgtcaataa
     3721 atttgaagca attttgttaa aaaataatca aaaatcgccg cacctggatc tcagcatttc
     3781 tatctccgct ttactgagat acctccattt cccttctcca agatctccgg cagttatgcc
     3841 cccgatagcc gtccttttca accgcaggac cggatgcccg atagtctcga acatcctccg
     3901 gacctgcctg tttttgcctt catgtatcgt tatttcaaca acagatcttc ctgctgactt
     3961 atcaatgatc ctaacgcacg ccggagcggt gatgtggcct cctatatcca gcccgttctc
     4021 aagacgctct atatcatccg cactaagcat gccttttatt tctgccctgt atactttttc
     4081 gacttcattt ctgggatgca tgagcatatt ggcgaactcg ccgtcatttg taagaatgat
     4141 cagccctgat gtatcgtaat cgagcctccc gaccggatat atcctttcgc tgatgccgct
     4201 tacaagatcg atgacggttt ttcttgaaaa ctggtccttt acggctgata tataacctgt
     4261 cggcttattc agcagaatgt agactttttt atgccctggg cgtattaaac ggccgtccac
     4321 tcttacttcc tcggagcctt cgactttggt ccccggttcg gtcactgttt tcccgtttac
     4381 ctgtactctt ccctgcagga tgagttcttc acatttccgc cttgatgata ccccggcatc
     4441 agccagatat ttttgaagcc tgatcacagt ccgtttcctt tcatacgttc tgctgtttta
     4501 tgtttcgtat aaaagcagtc catgaatccc gtaacccttt tatacccata aaatcatagc
     4561 ataaacccgc acttgcagaa aagatgcgtc agacgtctcc ggaccgatga ccaggctcaa
     4621 tggaagccgg catgctgata ttggattttt gaggatcatt ttatcactcg ggatcttctt
     4681 ccttttcctt ttttacggcg atcttctttt tgcagatatt ctcaagcctg cacatgaatt
     4741 caggtacaag gtccagtacc ttgtcgatgg tcgaatttat gttgaccggg aggagtttga
     4801 tatggccatt ccccacgacc atgaatgcta ccgggctgat gctcacgcct gcgccgctgc
     4861 ccccggcgaa tgggaatttt ccttcgatac cgctgctgcc atcctcatca tccgttttgc
     4921 tttcgtcatt ttcgtcctcc gcggcaccgt attcgccgcc gccggctgca aaaccgaaag
     4981 tgacttttga aatggggatg ataactgtcc cgtcaggtgt ttgcacagca tctcctacaa
     5041 tagtgtttac atcgaccatt tccctgatac tttccatagc tgttttcatc aacgcttcta
     5101 tcggatgttg ttcagccact tgctccacca cctgttgctt tttttgaatt ttttacgata
     5161 ctgtgtttca tccagtatat ttttatcaat acaactataa tatggaccaa cctcacatga
     5221 aatatgcagc gggcttccac ttcgaaaacc ttttcattga agcacggcgt gattttgatc
     5281 cttttgctta tggttttcag atggcgggac aaatatgcga caataatacc tgcagcgctc
     5341 cagagcgcac cgcataccag gccggtctgg gcggcgtcac cggttccctg tctgagtttc
     5401 acttctatct cgacatgtat gccatgcctg ttgatgtatt ctcttatgtc ttccagaagg
     5461 cttttatgat ccttcagata ttgcctcaca ctgatgaatt tttcgattat gtcttccggc
     5521 ccaagctttt catttgcttt tgaaccgccc tgcattttat ggctctgtcc cttgaccagc
     5581 ttgaatttcg tctttccgtt atcatttttt gccagtggca cctcaaactc gtatcttatt
     5641 attccctttc cggtataaaa gcagaaaatg acccattcat cctgctcgtc cctggtatat
     5701 tcaacggcag cctttatcct gacgacaaac agcacggtat ataaagcaag gataaaaaaa
     5761 acagctatgt aaagcatcgt tgactccatg tgatgaggta atgctccaca agctattttt
     5821 tccagtttgc ggctttttca tccttatata ttatttcatg ctttcataca gcagtcgtcc
     5881 tgtgcagtat ttctgcgtaa tggaactttg aacacggcct gtgcatcttc ctgtcctgca
     5941 tcatgtatcc gtgtcattct tctgcatatc ttcttcaggc tggttttcag atatcatcct
     6001 gtcgagttcg agcatcggca gatccttgat ggatctgaag ccgaaacttc tgaggaattc
     6061 ctcggtggtt tcatacagca cgggccttcc aggggaatcg agccttccgg cctcctttat
     6121 caggttcctt tcaagaagcg tggcgattga actgtctgaa ttgactcccc ttatcatctc
     6181 cacttttgct cttgtgaccg gctggttata tgcgattatc gcaagcgttt cgaatgcggc
     6241 ctgggagagc gcctgtttct gtcttggttc cgcaagtttg cttatgtact caaaatgctc
     6301 aggccttgta cataactggt agctgccgtt cagttcgcgt atcagtatgc ctctctgtga
     6361 gttttgtatc ctgatcgcca tattgtcgag aatccgtttt gcagttgctt tgtcaagctc
     6421 aagtatttct gcgatctttg ccagaggcac aggatctcct gccgcaaaaa ggacaccttc
     6481 cacgatcgct tccatttctt ttatatccat aactgactgc cttcctgcct cagtaatgtt
     6541 cattatccgg tcgattccat tatgatcatg ccatgctgtc aagctcgcgg gcatcgatct
     6601 ttccaagttc cggcgccatt tcgaccttac ttacgtatat ctcgccatgg attttcctct
     6661 gctcaagcct gaccctcctt agcctggcca gttcaagtat cgcaaggaaa cctgtcacga
     6721 catcggcacg ggatctcgtc ttgaatgaga acaattcaaa aaacctgaac ctgccccttt
     6781 ttatcagttc cctgatgatc tccctcatct tgcttctgag ggagactttt tcatgctgga
     6841 tgatgcggtt catcccgttg acattgggat tcatcttttt catgttcctc tccagcagtt
     6901 ccgtatagac ccttctcagt tcttcaggaa caagctccac gacttcatct tttctcacgc
     6961 atgtgatcgg ttccggcagc ttccaccata ccttcgccca ctctttttca cgctccctga
     7021 gcacatacga aaaatccttg tacttcctgt attccaggag cctcgatacc agttcctccc
     7081 tcgggtcttc ctcctgctgc tgttcctctt tcctggaagg gagcagcatc ttcgatttga
     7141 tatgaaggag cgtggaagcc atgacaagaa actcgctggc tatctccagg tccatctcct
     7201 gcatggtgaa caaatagtcc atgtactggt ccgtaatggt gtttatggga atatcataga
     7261 tattgaactg gtttttttct atcagatgga aaagcagatc aaaaggacct tcaaaattat
     7321 ctattttaat tgtacatgca ttggtaagcg cactttccaa gtatattccc ccggaattcc
     7381 ataacggccg gacgaccggt catgatcctt ttgcccttgc gtacttttcg aggtcggatg
     7441 catctctgca taaccgtgtt ttttcctgga tcgtcaccag tctatcttca tggcctttct
     7501 cacatcagct atcgtttttt ctgctgtttt ccttgcacgg gcattcccgt cctcgattac
     7561 tccctttata tatcccatat aatcaggctt gctgaggatt tcctgccttt tttcgtatat
     7621 gggcgtcagt ttttcagcca tctttgaata aaggttcttc ttgcactgga cgcatccgat
     7681 gcagcctgcc ctgcactgtg cttcagtttc ggaaactccg tcctcgtcga aaaccttatt
     7741 gaatgcgaat accgtacata cttccggatg gcccgggtca tccttcctga tcctcgcggg
     7801 gtcggtcacc atcgacatta cctttttgcg caccgtttca gggtcatcgg acagagcgat
     7861 ggtgttgttg tagctcttgc tcattttcct gccgtccgta ccgggcaata ctttcgcttt
     7921 tgtcagtatg gactgcggtt cagggaatac ttctccgtaa agataattga accttcttgc
     7981 gatctccctc gtcagctcga cgtgcggcac ctggtcctca ccgacgggca cggcggatgc
     8041 cttgtacatc aggatatcgg cagcctggag gcatggatat cccaaaaaac catatgtcag
     8101 tatattcttg tccttcaact gctgtatctg gtctttgtag gtaggacatc tgtacagcca
     8161 ggaaagcggc gttatcattg agaacagcag atgcagctcg gcatgttccc tgactgacga
     8221 ctggaggaac agcgtgcttt tttcgggatc aagaccgaca ctgagccagt ctatgaccat
     8281 attcgtgata ttggatccaa gttccgatgt atcctcatag ccggtagtaa gtgcgtgcca
     8341 gtctgctata aagtaatagc agtcatattc atcctgcagc tttacccagt tctcaagcgc
     8401 tccgaaataa ttgcccagat gcagtgcgcc tgtcggtctc gcgccgctga gcatgatttt
     8461 ccttcccgta tttgctctca tatgtgcctc gctttccatt cccatgatcg ctttttctta
     8521 actgaactta tacatattgt agtttcagca tccaaaggtg tcaagtgata atgtaccgga
     8581 taatgagcga tcctgccagg ccgaacagca attcgaccgc tctcctgaat ggatacgtaa
     8641 cggttgaaag tatgccgggc ctcagtaaca tcagcgccag aaacagcagg ccgatatatc
     8701 tttcatagcg catgaggccg aaatatatcc tcgcaggcag cactcccatc aggatctttg
     8761 acccgtccag cggcggtatc ggaagcaggt tgaatatggc aaggccaatg tttatcatgt
     8821 agaatatccc gcttatgatg aaaaccgctg ataccaatga gaaattctga ccatagaaag
     8881 ctatcaggta tggcatatat ctgctgtcat atataacatt gtacggcaat ccgcttttcg
     8941 aagcgatagc cgccattggg aacgaaaata taaaggccag caggagattc gaagcaggcc
     9001 ctgcaaggct taccatgaca gttcctttct tgtagttcct gtaataagta ggatctatcg
     9061 gcacaggctt tgcccagccg aacccgaatc cactgtacat cgaaaccacc agcataagcg
     9121 tccccagggg atccaggtgc ttcagcggat tcagcgtaag cctgccaagc ctttttgcag
     9181 tagggtcgcc cagtctgtaa gatatatatg catgggccat ctcatggaaa ctcagtgaaa
     9241 aaaggatggc aggtatcgca tataaaatca ttgctattct atccatatct ccgtatcatt
     9301 tccttccgta tccgggactg atatcagtca aaacatctct cgtttatcat gtggctgtcg
     9361 gcctctgaca ttccttcatc tgcctgctct tattttattt taccaaagat gatagtaaaa
     9421 gtctgtctaa aattatcttt acccttatgt ttatttgtca aaaccagtgc cgtttcgaac
     9481 ctgcggtttc agccgacggt tccccgcgcc aggccgtttt gaactgcagc cgcggcataa
     9541 caggagcctg gactggcaat gaaatatcac tgatgtgtat catcatgata aagacttatg
     9601 cataataata ccagcagtcg atctatgatc ttctgctggc tgtacaacga tctgatatac
     9661 tatgtgatat atactgcatc aggcattcag cctgctgtat cctgctgctg tattccgcct
     9721 ttatccgttg tatcctgccg tgttcagtgt tcagcttgcg gctctgccaa ggcagaacca
     9781 ttcctgcatg agccttaaca ttatgcgcag gaacgttgcc ttctggacat tgcttgacgc
     9841 cacgatatca gcccttccca cttcttttcc tgacagtgtg tatatcacct ctcctatttt
     9901 ctggccggac ttgaccggcg ccctgatatc agagctgata ttcaccactc ttgtacaatt
     9961 aatattgtat taaatcagtg accgaagcca ctgttcgacc ca
//
LOCUS       DAOSCC010000078         9922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4468_length_9922_cov_4.608797, whole genome shotgun sequence.
ACCESSION   DAOSCC010000078 DAOSCC010000000
VERSION     DAOSCC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4468_length_9922_cov_4.608797"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..684)
                     /locus_tag="PLG72_05670"
     CDS             complement(46..684)
                     /locus_tag="PLG72_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ80321.1"
                     /translation="MNIYIASLFDGFWLNIKLFVLTLAMAIPLGLVITFGSMSKFRPL
                     KWVVRTFVWIIRGTPLMLQLFVVLYSPGLLFDTPMKSRMTAAIVAFVVNYAAYFSEIY
                     RGGIESIPKGQYEAGAVLGMTKSQVFFKVILLQVIKRIVPPMSNEIITLTKDTALARV
                     IGIAEIIMCAERFTKQGLIWPLFSTGLFFLVFNGVLTIVLGWLERKLDYFRV"
     gene            complement(775..1608)
                     /locus_tag="PLG72_05675"
     CDS             complement(775..1608)
                     /locus_tag="PLG72_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ80322.1"
                     /translation="MRKITKTMSLLLVLLMLAAILPGCSSKAGSDYKYIKDKGTLVIG
                     ITQYPPMNYYDKEGKLVGFDTELAEIVCEKLGLKAEFVEINWDSKEVELNSKNIDCIW
                     NGMCITEERKQNMSISIPYMKNEQAIVVRAEREAEILSNVDGLILTAEAGSTGEGKAL
                     GTIPDDGTEDVSAKEFFANCEYVPADSMAKALMEVKSGTADMAIVDAVLAYYNVGPGT
                     DFEDLVANTDNNFGAQQFGIAFRKGSDMTEKVNKILKELMEDGTMAEISKRYGLEDSL
                     L"
     gene            complement(1893..3005)
                     /gene="glf"
                     /locus_tag="PLG72_05680"
     CDS             complement(1893..3005)
                     /gene="glf"
                     /locus_tag="PLG72_05680"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HOJ80323.1"
                     /translation="MKSKYDFLIVGSGLYGAVFAHELKKAGKTCLIIEKRSHIAGNVC
                     TEEVEGIHVHKYGAHIFHTGNREVWQYVNQFAVFNNYVHSPVANYKGEIYNLPFNMNT
                     FNRMWGVVTPEEARKKIEQQISSDFTEHPGNLEEQAINLVGRDIYEKLVRGYTEKQWG
                     RPCSELPAFIIRRLPVRFTYDNNYFNDAYQGIPEGGYTAMVERMIEGIEVRLNTDYLA
                     EKSKWDSIADRVVYTGPIDAYYSYRFGALEYRSLRFETEILDMQNYQGCAVVNYTDSE
                     TPYTRIIEHKHFEHGTQPKTVITREYSLEWKPGAEPYYPVNDEKNNALYDKYRALAEN
                     QQNVIFGGRLGEYRYYDMDKTVEAALKKVREVLGNI"
     gene            complement(3040..4056)
                     /locus_tag="PLG72_05685"
     CDS             complement(3040..4056)
                     /locus_tag="PLG72_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ80324.1"
                     /translation="MKVLSVAVPCYNSQDYMRYCIESLLPGGEDAEILIIDDGSSDDT
                     GNIADEYANKYPSVVKAIHQENGGHGGAINTALKNASGLYFMVVDSDDWVDVRSYLKV
                     LETIKRLSENGNQVDLFISNYVYEKDGKRFKKVVRYDDVLPEGRIFTWDEIKPFKKDQ
                     YISMHSAIYRTRLLRDCGLELPKHTFYVDNLFVYLPLVHVKKLYYINEDLYRYLIGRK
                     DQSVNEQVMIRRIDQQVKVNKIMIDQVNLENVENEKLRQYMLNFLEIITVISSFFLIL
                     SGTPENLEKKKELWEYIKGKDLQAYKEIRSGTLGRLINLPGRPGRDISLLVYRVSQRL
                     IGFN"
     gene            complement(4394..4470)
                     /locus_tag="PLG72_05690"
     tRNA            complement(4394..4470)
                     /locus_tag="PLG72_05690"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4434..4436),aa:Met,seq:cat)
     gene            complement(4661..4748)
                     /locus_tag="PLG72_05695"
     tRNA            complement(4661..4748)
                     /locus_tag="PLG72_05695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4712..4714),aa:Leu,seq:taa)
     gene            complement(4818..5243)
                     /locus_tag="PLG72_05700"
     CDS             complement(4818..5243)
                     /locus_tag="PLG72_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ80325.1"
                     /translation="MQVEVGSIVEGKVAGITSFGAFIQLPDGKTGLVHISEIAEEYVK
                     DIKDHLKENQQVKVKVLSMENGKISLSIKKAAEPKETRKTSSIPAEIDWNRNGSENLS
                     FEDRLAKFLKDSEEKIHDLKKNFESKRGSGGYRRTTQYF"
     gene            complement(5399..5674)
                     /locus_tag="PLG72_05705"
     CDS             complement(5399..5674)
                     /locus_tag="PLG72_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ80326.1"
                     /translation="MKKGKATVGIFILLVIFLYLSYIAVNQQGLIYAKNQELNKLEEK
                     IAEEKKINEELKKQSKMIESDEYIEKVAREKLGMVKKNERVYVDIGN"
     gene            complement(5807..6430)
                     /locus_tag="PLG72_05710"
     CDS             complement(5807..6430)
                     /locus_tag="PLG72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOJ80327.1"
                     /translation="MILSVSEQVIVFLWTTVSGMAIAFVYDIFRIIRKAVRTGGFAVF
                     VQDILFWLIACIIMFISIYRSNDGELRGFLFFGAFLGAVLYAMLFSKAVMGSSLFIIN
                     VTARAVKILIYIVLYPFRFLVKLLSKPVRMAAKAAADSLRMAREREKEMPGSKTGVSD
                     SKTSGRDSALSRIAGFLRKRPERRPRRRRRREKDEALNTVSNVDLQR"
     gene            complement(6427..6720)
                     /gene="yabP"
                     /locus_tag="PLG72_05715"
     CDS             complement(6427..6720)
                     /gene="yabP"
                     /locus_tag="PLG72_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOJ80328.1"
                     /translation="MIEEKKMPKPRVQNVILENRQKLSISGVVDVESFNDECVIADTE
                     LGILVIKGMDLHINKLDIDSSELGIEGDIISLEYSDREGERNKGGGFFARLFR"
     gene            complement(6909..7148)
                     /locus_tag="PLG72_05720"
     CDS             complement(6909..7148)
                     /locus_tag="PLG72_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOJ80329.1"
                     /translation="MRIDKYLKVSRLIKRRTLANEACETGHVTINGKTAKPGTDVKVG
                     DVIEIRFGNNLTKVRVLSTEEHVTKEGAKEMYTIL"
     gene            complement(7172..7447)
                     /locus_tag="PLG72_05725"
     CDS             complement(7172..7447)
                     /locus_tag="PLG72_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ80330.1"
                     /translation="MNKSELIGRMAEKGEITKTDAEKALNAFLESVEEALANGDKVQL
                     VGFGTFEVRERPERKGRNPQTNEEITIEASKAPVFKAGKALKDIVNS"
     gene            complement(7466..8266)
                     /gene="mazG"
                     /locus_tag="PLG72_05730"
     CDS             complement(7466..8266)
                     /gene="mazG"
                     /locus_tag="PLG72_05730"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ80331.1"
                     /translation="MKLKEKYGFDDLLEIMARLRGENGCPWDKEQTHESLRIYMIEET
                     YEVLEALDSGDKGRFCNELGDLLLQIVFHAQIAKENGDFDIGDVITEVCKKLISRHPH
                     IFGNVKADTAEEVIENWEAIKKKEKKLKSQTGVLKDVPKNLPALMRSYKVQQKAAQVG
                     FDWDNIEDVFRKVEEEIQELRDVYKSNNVERITDELGDAFFALVNLARFLNVQPELAL
                     NGTIGKFIKRFEYIEQQSMKAGKKLEEMSLSEMDALWNEAKIQLSDKQ"
     gene            complement(8403..9812)
                     /gene="uxaC"
                     /locus_tag="PLG72_05735"
     CDS             complement(8403..9812)
                     /gene="uxaC"
                     /locus_tag="PLG72_05735"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOJ80332.1"
                     /translation="MKKKFMDDNFLLHNETAAILYHEYAAKMPIIDYHCHLSPREIAE
                     NKKFRNITEIWLGGDHYKWRAMRINGVEEKYITGDADDKEKFMKWAETMPYCIGNPLY
                     HWTHLELRRYFGIEELLSPETAEYIWDKCNEMLADDSFSARELIKRSNVKALCTTDDP
                     ADTLEYHERIADDKGFGVKVLPTFRPDAALNIDRPGFTQWVTKLEKAAGKKISGYDSF
                     RDALSGRLDYFNSRGCRLSDHSLEPAVYAECSDEEASRIFDKVMSGGKPDTEEANKYR
                     TNLLTFLGARYSELGWVMQLHMGCMRNNNTRQFRLLGPDTGFDAIDDAVYARPLAKLL
                     DRIDTVAGIPKTILYCLNPGANEVISVIAGCFQGGGIPGKIQFGSAWWFNDQLDGMKR
                     QMTALASTGLLSRFVGMLTDSRSFLSYARHEYFRRILCNILGEWAENGEAPDDTALLG
                     KMVQDISYNNAKDYFGFGI"
BASE COUNT         2263 a         2647 c         2050 g         2962 t
ORIGIN      
        1 tttgcatata tttttgactt cgagtattgg catcagtcag acctcctaca ccctgaaata
       61 gtccagcttg cgttcaagcc agcccaggac gatcgtaagc acgccgttga aaaccaggaa
      121 aaacaggccg gtcgagaaca ggggccagat cagtccctgc ttggtgaacc gttcggcaca
      181 cattatgatc tcagctatcc ctattaccct cgccagtgca gtatccttcg taagcgtgat
      241 gatctcgttg ctcatcggag gcactatcct cttgatgacc tgcaaaagta tgactttgaa
      301 aaatacctgg gatttggtca tgccaagaac tgccccggct tcatactgcc ctttcggtat
      361 gctttctatc ccgcccctgt atatttcgga gaaataggcg gcataattga cgacaaaggc
      421 cacgatcgcc gctgtcatcc ttgacttcat cggggtatcg aacagcagcc ctggagaata
      481 cagcaccaca aacagctgca gcatcagcgg cgttccgcgt atgatccaca caaaggtcct
      541 gacgacccac ttgagcggtc tgaatttcga catggatcca aaggtgatca caagccccag
      601 cggtattgcc attgccaggg taaggacaaa cagcttgatg ttgagccaga atccgtcgaa
      661 gagcgatgca atgtatatgt tcatacagtt cctccgaata atgatgaaat ctccactggg
      721 atcacgaaga ccccaatgga gacttgcctg tgatgcccgt acgatatgcc gatcttacag
      781 aagactgtcc tccaggccat aacgtttgga tatttcagcc attgtgccgt cttccatgag
      841 ttccttgagt atcttattga ccttttcggt catgtcgctg cctttgcgga aagcaatgcc
      901 aaactgctgt gctccgaaat tgttgtcagt atttgcaacc agatcctcga agtcggtgcc
      961 gggtccaacg ttataatagg cgagcactgc atccacgatc gccatgtcgg cagtaccgga
     1021 tttgacttcc atcaacgcct ttgccatcga atcagccggt acatattcac agttggcaaa
     1081 gaactccttt gcggatacgt cctccgtacc gtcatccggg atcgtaccca gggccttgcc
     1141 ttctccggtg gatccggctt ccgccgtcag tatcaggcca tcaacattag acaggatctc
     1201 cgcttccctt tctgctctca caacgatagc ctgctcattc ttcatgtagg gaatggaaat
     1261 gctcatgttc tgcttgcgct cttcagtgat gcacataccg ttccagatgc agtcgatatt
     1321 tttggagttg agctcgactt ccttggaatc ccagttgatt tcgacaaatt cggctttaag
     1381 tccaagcttt tcacatacga tctcagccag ttcggtatcg aagccgacca gtttcccttc
     1441 cttatcatag tagttcatgg gtggatactg ggtgattcca atcaccaggg tacctttatc
     1501 ctttatgtac ttgtaatcag aaccagcctt cgacgagcat cccggcagga tcgctgcaag
     1561 catcagcaat accagcagca aagacatcgt ttttgttatc tttctcatat catgttcccc
     1621 ctcgcaacat tttgaccaag ttctttgtat caatttacca cattgtcact ttagtgtgct
     1681 aaatcgatga tatatttata ccacaggttt aatataaaat caatagtcaa aatttaattt
     1741 atttttaact gttgagcaat tggagaagta atctctaccc atgcatcaca gacagcgctt
     1801 tattgcttct gacgcaagaa acgccgggac tggctgcccg ttgtcagcgg ccgacatgga
     1861 tacgtcaaca gaggcgttga agaaaaaacc tgctatatat tcccaaggac ctctctcacc
     1921 ttcttgagag cggcttccac ggttttgtcc atatcgtagt acctgtactc tcccaaacgt
     1981 ccgccgaaga tgacattttg ctggttttcg gccagtgccc tgtatttatc atacaaagca
     2041 ttattctttt catcgttgac cggataatag ggctcagcgc ccggtttcca ctccaggctg
     2101 tattcccggg tgatgaccgt tttgggctgt gttccgtgtt caaaatgttt atgctcgata
     2161 atgcgcgtgt acggagtttc gctgtcagtg taattcacca ctgcacagcc ctggtagttc
     2221 tgcatgtcca gtatttctgt ttcaaaccgc aggctcctgt attcaagggc tccgaaccgg
     2281 taactgtaat aagcgtctat aggacctgta taaactaccc tgtccgcaat actgtcccac
     2341 ttgctttttt ccgcaaggta atcagtgttg agcctgacct cgatcccttc gatcatcctt
     2401 tctaccatgg cggtgtaccc gccttccggt atcccctggt aagcatcgtt gaagtaatta
     2461 ttgtcgtaag tgaatctcac cggcaacctg cggattatga atgccggcag ttcgctgcac
     2521 ggacggcccc attgcttctc agtgtacccg cggacaagtt tctcgtatat atcccgcccg
     2581 acaaggttta tggcctgttc ttcaaggttc ccggggtgtt cggtaaagtc ggagcttatc
     2641 tgctgttcga tcttttttct cgcttcttcc ggggtcacga ctccccacat cctgttgaac
     2701 gtattcatat taaaaggcag attgtaaatt tcgcccttgt aattcgcaac aggactgtgg
     2761 acataattgt tgaacacggc aaactggttt acatattgcc atacttccct gttgccggta
     2821 tggaaaatgt gggcgccgta tttatggaca tggatccctt ctacttcttc agtgcagaca
     2881 tttccagcga tatggctgcg tttttcgata ataaggcatg ttttccctgc tttcttcagt
     2941 tcatgcgcga aaacagcccc gtataagcct gatcccacga tcaggaaatc atacttgctc
     3001 ttcattgcag cgatcctcca aaacaggtat gtatactgtt cagttgaatc ctatcagtct
     3061 ctgggatacc ctgtacacca gtaaggaaat gtcccgtccg ggcctcccgg gcaggttgat
     3121 caggcgccca agcgtcccgg acctgatttc cttatatgcc tgcaaatctt tgccctttat
     3181 atattcccac agttcctttt tcttttccag gttctccggt gttccggaca gtatcaggaa
     3241 gaatgaagaa attactgtga tgatctcaag gaagttgagc atatactgcc tgagtttctc
     3301 attttccacg ttttccaggt tcacctggtc tatcatgatc ttgtttactt taacctgctg
     3361 gtctatcctt cttatcatga cctgctcgtt taccgactgg tccttcctgc cgatcaggta
     3421 cctgtacagg tcttcgttta tgtaatatag ttttttcaca tgtacaaggg gaagataaac
     3481 gaataaatta tcaacataaa atgtatgttt cggaagttcc agcccgcaat ccctcagcag
     3541 ccgcgtcctg tatatggccg aatgcatcga tatgtactgg tcttttttaa agggcttgat
     3601 ttcgtcccat gtgaatatcc gtccttccgg aagcacatca tcatatctga caactttctt
     3661 gaatcgtttg ccatccttct cataaacata attgctgata aacaggtcga cctgatttcc
     3721 attttcgctt aatctcttta tcgtttccag tacttttaga tatgatctta catcgaccca
     3781 gtcatcgctg tccactacca taaaatacag gccggaagca ttcttaagag cggtgtttat
     3841 tgcccctcca tgcccgccgt tttcctggtg tatggctttc acaacggatg gatatttatt
     3901 ggcatattca tctgcgatat ttcccgtatc atctgaagaa ccgtcatcga ttataagtat
     3961 ttctgcatct tccccacccg gcaaaagcga ttcgatgcag tatctcatat aatcctgcga
     4021 attatagcag ggaactgcta ctgacagtac tttcatatct acacctcact actgaccgct
     4081 ttgcttcatt tttccgcttt catcagtcaa tatattccat ccgggaaaca caatatctgc
     4141 aatataaata cgtaaacagc caaaaatttg ctcatttata taccatattc tataatcgta
     4201 caatagctga aaaaagtcaa catttagttt ccctgaattt gagacatgtc ctaagaatac
     4261 tcacggtgtg tattcgttcc ggatccggaa ggatatgtta aagttttttt gtcagatcgg
     4321 ccgcaagtac ccggagtgta cttataatac gacagcgcct tccaggtgaa aacctgtaag
     4381 gcgctgcttc ctttggttgc gggagaggga tttgaacccc cgaccttcgg gttatgagcc
     4441 cgacgagcta ccggactgct ccaccccgcg atatatttcg ctgctgagtt ttcctgtatt
     4501 gattcaactc aactgcgctt atatagtata acaccacaga gcgttttatg caagttttcc
     4561 ggcgaatttt tttcaggcaa tacatagcaa tgcaggtgtc ttttcagtgc cttccaggcg
     4621 ccggagcata aaaacgggtt tcggcaaaac ccgaaacccg tggtgccgaa gaccgggatc
     4681 gaaccggtac ggctttatgg gccgcaggat tttaagtcct gtgcgtctgc cagttccgcc
     4741 acttcggcat ttataacaat tacatatgat aacacctgct gtcttaaaaa gcaagtgcag
     4801 ctgaaaggca aatgtttcta gaaatattga gtagtcctgc gataaccgcc gcttcctctc
     4861 tttgactcaa aattcttttt caggtcgtgg atcttttctt cgctgtcttt caggaatttc
     4921 gccagccggt cctcaaacga aaggttttcg ctgccgttgc ggttccaatc tatttcagca
     4981 gggatactgc ttgttttcct ggtttcttta ggttcggcag cctttttgat cgacaggctt
     5041 attttgccgt tttccataga taatactttt actttgacct gctgattttc cttcagatga
     5101 tccttgatgt ccttgacata ttcttcagcg atctcggaga tgtgaacaag acccgtcttc
     5161 ccgtcaggca gttggataaa agctccgaaa ctggttatcc cggcaacttt accctctact
     5221 atgctaccaa cttcaacctg cataaagaaa aaaatcctcc ttaaatttaa aaaaacggtc
     5281 aatgccgttt aatgcgaccg gctgcaaatc ttccaaagca attcgattat atagcatgcg
     5341 catgctttcg tcaagcttgt tatcacaggc ttttaaagat ttttggacat aactccggct
     5401 agttgcctat atcgacatag actctttcat ttttctttac cattcccagt ttttcccggg
     5461 caactttttc gatatactca tcgctttcga tcattttgct ttgctttttc agttcttcat
     5521 ttatcttctt ttcctcggcg atcttctctt ccagcttgtt aagttcctga tttttggcat
     5581 aaatcagccc ctgctggttt acagcgatat aggacagata tagaaatata acgagcaaaa
     5641 tgaatatccc gaccgtagct tttccttttt tcatatgtat ctctacctga tccgaaagaa
     5701 tcagtcccct gtcttttaca tttccggcct gtttccgggc tgtatattac cgtttgctat
     5761 cttccataca tatagtataa ttctatgctt tctgcaaaaa tccttcttac cgctgcaaat
     5821 ctacattgct gaccgtattc aaggcttcgt ccttttccct tcgtctcctt cgccgcggcc
     5881 ttctttccgg tctcttacgc aggaatccgg caattctgct gagtgcactg tctctgcctg
     5941 acgtcttgct gtcagatacg cctgtcttgc tgcccggcat ctctttttcc ctttcccttg
     6001 ccatacgtaa agagtccgcc gccgctttgg ccgccatgcg cacaggtttc gacagcagtt
     6061 tcaccaggaa cctgaaggga taaagcacga tataaatcag gattttgacc gccctggccg
     6121 ttacgttgat aataaaaagg gaggagccca tgacagcttt gctgaacagc atggcataca
     6181 gcacggcgcc gagaaacgcc ccaaagaaca ggaaccctct cagttcaccg tcattgctgc
     6241 ggtatatgct gataaacatg attatgcagg ctatgagcca gaacagtata tcctggacaa
     6301 agacggcaaa accgcccgtc ctgacagcct tcctgataat cctgaatatg tcatagacga
     6361 atgctatggc catcccgctt actgttgtcc aaagaaaaac aatgacctgt tcgctgacag
     6421 aaagtatcat ctgaataacc gggcaaagaa gcctccgccc ttgttcctct ccccttctct
     6481 gtccgaatac tcaaggctga tgatatcgcc ttcgatgcca agttccgagc tgtcgatatc
     6541 cagcttgttt atatgcaggt ccattccctt tatcaccagt atgccaagct cggtatctgc
     6601 gatgacacat tcgtcattga agctttcaac gtcaacaacg ccggatatac tcagtttctg
     6661 cctgttttcc aggataacgt tctggaccct tggctttggc atcttcttct cttcgatcat
     6721 accgaccctc cccgccactg tttgctcctc cggtcacgtt gaaatgccca ggcgatgcca
     6781 cattaaatat atatgcatgt acaatgcata tatgccggca ggacgaacga aaaaatccct
     6841 tcagggccga tgcctttgcc cttcagggat tttgacgcat accggttcaa ttgccttaag
     6901 gatcagtatc acagtatagt gtacatttcc tttgcccctt cttttgtaac gtgttcctcc
     6961 gtcgacaata ccctgacctt tgtaaggtta ttgccgaacc ttatctctat tacatctcca
     7021 actttcacat cagtccccgg cttggcagtc ttgccgttta ttgtcacatg tcctgtttcg
     7081 caggcttcat tggcaagggt gcgccttttg ataagccttg agactttcag atatttgtcg
     7141 atcctcataa aatacatccc ccatatggaa tctaactgtt gacgatatct ttcaaagcct
     7201 tgcccgcttt gaacaccggc gccttcgacg cttcgatcgt tatctcttca ttggtctgcg
     7261 ggttccttcc ttttctttcg ggcctctccc tgacttcaaa agtcccgaag cctacgagct
     7321 gcactttgtc cccgttggcc agggcctctt cgacgctctc caggaaagcg ttcaaagcct
     7381 tttcggcatc cgttttggtt atttcgcctt tctccgccat cctgccgatc aattcagatt
     7441 tgttcatccg tctacctcct gattctcatt gcttatccga gagctgaatt tttgcttcgt
     7501 tccagagtgc atccatttcc gaaaggctca tttcctccag ctttttgcct gctttcatgc
     7561 tttgctgttc gatgtactca aaccttttta tgaacttgcc gatggttccg ttaagagcca
     7621 gttcaggctg cacattgaga aatctggcga ggttaacgag agcaaaaaat gcatctccca
     7681 gttcatctgt aattctttcc acattattac tcttatatac atcccgcagt tcctgtattt
     7741 cctcttcgac tttccggaaa acgtcttcta tgttgtccca gtcaaaaccg acctgcgcag
     7801 ctttctgctg caccttgtag cttctcatca gggcaggcag gtttttgggc acatccttga
     7861 gcaccccggt ctggcttttt agcttctttt ccttcttctt tatagcttcc cagttttcga
     7921 tgacctcttc tgcggtatca gcctttacat tgccgaatat atgaggatgc ctggatatga
     7981 gctttttgca gacctcggta atcacatccc cgatatcgaa atcgccgttt tccttcgcta
     8041 tctgtgcatg gaacactatc tgcagaagca gatcgcccag ttcgttgcag aatcttccct
     8101 tgtccccgga gtccagcgct tcaagtactt cgtaggtctc ttcgatcata tatatccgca
     8161 ggctttcatg agtctgctcc ttatcccacg ggcaaccgtt ttctccccgg agccttgcca
     8221 ttatctcaag caaatcatca aacccgtatt tttctttgag cttcatatgt atctcccgtc
     8281 actttctgca aattcagcgt attcctgctt tattttatcc taattttatc acaaatagca
     8341 ataaaaaacc cgcccgaaag tcaaaccgga cagccgcagg cgggttgacg gaaaacacgc
     8401 agtcatatgc caaatccgaa gtaatccttt gcgttgttgt aactgatatc ctgcaccatt
     8461 ttcccgagca gggccgtatc atccggagcc tcaccgtttt cggcccattc gccgaggata
     8521 ttgcacaata tccgcctgaa gtactcatgc ctggcgtatg acaaaaaact tctcgaatcg
     8581 gtcagcatcc cgacaaacct gctcagcagg ccggtggatg caagcgccgt catttgcctt
     8641 ttcattccgt ccagctggtc gttgaaccac caggcggagc cgaactgtat cttcccgggg
     8701 atgcctccgc cctggaagca gccggcaatt acgcttatga cctcattcgc gccgggattg
     8761 aggcagtaca ggatcgtttt cgggataccg gccacggtat ctatcctgtc cagcagtttc
     8821 gccagcgggc gcgcataaac agcatcatcg atggcgtcga agcccgtatc gggcccaagc
     8881 agcctgaatt gccgcgtgtt gttgttcctc atgcatccca tgtgtagctg catcacccag
     8941 ccaagttcgc tgtaacgggc tcccaggaag gtgagcaggt ttgtcctgta tttattggct
     9001 tcctcagtat ccggtttccc gcccgacatt accttgtcaa aaatccgcga agcctcttca
     9061 tcgctgcatt ccgcgtatac tgcgggctcg agcgagtggt ccgacagcct gcagcccctg
     9121 ctgttgaagt aatccagcct gccggagagg gcatccctga aactgtcgta gccgcttatt
     9181 ttctttccgg cggctttttc aagcttcgtc acccattgcg tgaaaccggg cctgtcgatg
     9241 ttcaacgccg cgtcaggcct gaaagtcggc agcaccttaa caccgaatcc tttatcgtcg
     9301 gctatccgct catggtattc aagcgtgtcg gcagggtcgt cggtggtgca caaagctttc
     9361 acatttgaac gctttataag ctccctggct gagaatgagt cgtccgcaag catttcattg
     9421 catttgtccc atatgtattc cgcagtttcg ggtgaaagaa gttcttctat gccgaaatac
     9481 ctgcgaagtt ccaggtgggt ccagtggtac agcgggttgc cgatgcaata tggcatcgtc
     9541 tccgcccatt tcatgaattt ttccttatca tccgcatctc ctgtgatgta tttctcctcg
     9601 acgccgttta tcctcatggc acgccatttg taatggtcac cgccaagcca tatttccgtg
     9661 atgtttctga actttttgtt ttcagcaatc tccctgggac tcaggtggca gtgatagtcg
     9721 atgatgggca ttttagcggc atattcatga taaaggatgg ctgctgtttc attatgcagc
     9781 aggaagttat cgtccatgaa ttttttcttc ataacccatt ctcctttaag tcagaatgtc
     9841 tggtattctg cgcgtgtatc agcacacagt cgcccgttaa gcggactgct gaatgtcatc
     9901 tgcgcctggc accccatgaa tc
//
LOCUS       DAOSCC010000079         9826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4527_length_9826_cov_5.209497, whole genome shotgun sequence.
ACCESSION   DAOSCC010000079 DAOSCC010000000
VERSION     DAOSCC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_9826_cov_5.209497"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLG72_05740"
     CDS             complement(<1..1311)
                     /locus_tag="PLG72_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80333.1"
                     /translation="MKKKFFICILILSLLLPNFTTAMDDTRYYETSCKWETYFDNPEL
                     FEKLSGLPYGVTPVAKGRPFNYRIWFGKGIVVYGTPEEANTNESYRKNYKEDTLNENG
                     IGFYKKPGYKRGEYRYDGYTIEGNLYANTNFPPDVKRITPLEDIAWIYHYWDSKYVKD
                     IFKGEPVMRVSIYNKAAINNVSEIEKRTREWINNGITEFKIRNGVTDEGLKHTAPERK
                     PDGTWDFHHFLNVQSAPSAKFNGEGRMFRFDSKGKLFYMGFTIKKYQKEHTPVEVSVK
                     VINRDELNFIDYGKNNPEGFEKQVIGVKVEVTARLKDEKHMEDPLARAVYYTREDREK
                     WEVSLNGEAAPQEDIQIFDNMAKAIFVVPMTKGQIKALEGRNFSFRATARCIYFDGRY
                     DEGSGQCNANFDIEEYVVKNEPPFLMEPKCVIPQTGFDIVEFVAY"
     gene            complement(1349..2644)
                     /locus_tag="PLG72_05745"
     CDS             complement(1349..2644)
                     /locus_tag="PLG72_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ80334.1"
                     /translation="MYGRRFVWHGRHVIRICTEIILMAMLLGILALFPPTRAYAGESE
                     GTYENETAFRIKPLQEKLAELKDEEIENLLDSYGDMQSHWSRKEVGMLSLLGIINGDN
                     GMFHPDDPVQVDQFLKMTVRSMGFAPGENTKYWAQNYIDTALQQKLIAKGEFSDYRRP
                     ITREEATRIIVKATLLKEEFPYNDPYNNPDNLVRSKIKDYAKIKDENKQYVLQSYELG
                     LIRGAGGWFRPADTLTRAEAATIMIRYLDNASRVPFTPAEDEVFKCVNYDGSVVTAWP
                     PSKKEIIDAANAFRNVGQKSKGFVVSGFSEVGHNIFYDCYESEEKFKENKILNIQMAI
                     YFNTIDDESIMDNPYHIVLYDAEAVQRLHRDIVYEMFKFWFKDEANKAMAEYDRYLEY
                     AVCGDQKHRVEEITYNGREMFFYKVGGDNGFSLSIHSLK"
     gene            complement(2602..4200)
                     /locus_tag="PLG72_05750"
     CDS             complement(2602..4200)
                     /locus_tag="PLG72_05750"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HOJ80335.1"
                     /translation="MSGVAAEAVKELEFIYESDAASSFLVIRCAGNIIGYQAGMFENN
                     DIEYVIPMEIVKKEDANYFYYNITSRIPLSIYLKRNKLSREAFLRLILNITACISNSA
                     GYLLYPSNFLMKPEFIYIDPGTLDVMTVYIPAEFRHDDAGSLQTLVSELLMQHINEEG
                     FSSGNLVQRILSEVKSETFSIRGLITLVNELLYSPDIREKQKPPYEPEICIKMAEENR
                     EKYKKEEKKEAMENIRKKKPSPAVVIAVLLQLAMGIAIYMCRGIIAGTGNDATVNYIA
                     VALIILAIEVLIFRKLQAMKLFSIRDIVSTRPDKDGEPDAIPGEAMAQNAFIQTTGHM
                     RKSPADCETDSRKNAIAEKAAARNDISIPQGESAEEIRTDLKAKDAALPPESGVTCFS
                     QKTESLEMQNESEFVLINMNGQPGEKNIVIDRDEFIVGRLAGHVDHILYSNAVGKLHA
                     VLIRRNGCCYVKDLNSMNGTYINGKRIECNKEMLLKNNDILRLANCEFIFSCRQENKP
                     VKQDNDKEAEICMAEGLYGMAGML"
     gene            complement(4256..4471)
                     /locus_tag="PLG72_05755"
     CDS             complement(4256..4471)
                     /locus_tag="PLG72_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HOJ80336.1"
                     /translation="MNKLVKSFLSEEDGLGTVEIVVIIAVLVGIALIFRDAIIKFVTD
                     IMKSIFASDSVTNDVNSQLIRQNNQIN"
     gene            complement(4489..5349)
                     /locus_tag="PLG72_05760"
     CDS             complement(4489..5349)
                     /locus_tag="PLG72_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ80337.1"
                     /translation="MAVFFAVLAIIYLILYFLSRGRYADIICVLDKKEFPLKGLLPVS
                     MLILDTFKHSYNTGYDRKLAMAVAELYGHRKALLMLRVHWGNKISLVLLALMFAGFVG
                     IFADIDGGYLFFCVLLVVAAVVLSDRELHEKVRKRRQAIQLEFPDFVNKLTLLVNAGL
                     TVSKAWEKAAMDSQRETPLYKELCITVQAVRSGVSEHKAYEEFAKRCHVPAVTRFVTV
                     VLQNIRKGNSELVPVLRLLSDECWEMRKNTAKKFGEEASTKLLLPMMLMFIAILLIVG
                     MPAVLALRNI"
     gene            complement(5537..6280)
                     /locus_tag="PLG72_05765"
     CDS             complement(5537..6280)
                     /locus_tag="PLG72_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ80338.1"
                     /translation="MTDYNVYVMGTKQKMLYILAAAAVIFAAGFLFYRNVLISVLLTP
                     LALLYPGIKAKDLVKRRKKELNIQFKDLLYSLASSVSAGKSIESAFRAALEDLSVLYP
                     EPSTYILAEIRNIISRLDTNETLESALTEFAGRAGLEDLDNFVNVLSISKRTGGNIAQ
                     IIRNASSIISDRIEVGQEIDMLLAERRFEQKVLNAVPVFMIALLSASAPDYMYPIFNT
                     AAGRLTMTIALAMMVLAWFISAKLNDIKV"
     gene            complement(6277..7602)
                     /locus_tag="PLG72_05770"
     CDS             complement(6277..7602)
                     /locus_tag="PLG72_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOJ80339.1"
                     /translation="MRTEIAQELDRTRDIDDDELMEMVTAAVFRRSRVSWLNIAQKRE
                     LADMVYNSMRRLDILQPLLDDKSITEIMVNGPDKIFIERAGRIYRTEAAFADQQKLED
                     VIHNIVSRTNRTVNEASPIVDFVLPDGSRVNVVLKPVALNGPIMTIRKFPDKPMTLEQ
                     LTEAGSLSVEAAEVLDCLVKAKYNIFICGGTGSGKTTFLNALAARIPPDERIITIEDS
                     AELQIIGVDNLVRLETRNANSEGKGMITIRDLIRTSLRMRPERIIVGEVRGEEALDML
                     QAMNTGHDGSLSTGHANSAEDMLKRLETMVVSAAPIPLDAVRQQIASAIDIMVHLARL
                     RDKSRRVLEISEVAGYSNGRIILNPLFVFEEAVPCSSGGLDICIGDTDADNTGTGYAI
                     RPYVCRTAGVQAAESQPVTGCLKWTGNMLTRTFKLKMAGIRCRLGGGMQ"
     gene            complement(7673..8821)
                     /locus_tag="PLG72_05775"
     CDS             complement(7673..8821)
                     /locus_tag="PLG72_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80340.1"
                     /translation="MGRLSLVIADFDAEYIRNFGKYLIINYPGRFELTSFSSPQVLHG
                     FLDSPKSADIILVNSLMYDEKIGQYDRGLMLLLEEGSPVQAPQCRDSAPVSIMKYQHI
                     DRLVSEILRIYSTSTDRDHPLKGRKNTCIVSVASPAGGVGKSSIAAGCSILCAGMGFR
                     TFYLNLEITPSTDSFFRGESDQSFSNVIYHLKGSNSLWLRLESAKCRDIRSGVYFYKP
                     PESILEMNEFDEQDAVRLIREFRSSCVYDMVFVDLPSGLSNVNAEVMRHSDAIVLVLA
                     LDPVIESKAVIFIKGLDLLENRWKAALADKLLPVLNFSSGFAEYGAVYGYKPAAVIAD
                     CSRMHDGTAAGSLIGETSFLKSLNSIVEQILARRPPAGVLTDGGGSVA"
     gene            complement(8973..9584)
                     /locus_tag="PLG72_05780"
     CDS             complement(8973..9584)
                     /locus_tag="PLG72_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ80341.1"
                     /translation="MGPAEGSVITMRHYSMINVFDSILEKIIDDQIVVKLPKECRKAT
                     FLEGDPLVTAYELDEKAVIRGARIVDYNKDESLLTYREDVPDEGSRLRSYDRFPVSLY
                     ADYRVAGTAGNRKYYALVKDISDYGLMLYAKEEHYKGHKLNLDIYLARDILSLTAEIV
                     RKADHNGYFEYGLKIRHNGPIVFNQIKNFVRKEQEELIGKFSK"
BASE COUNT         2354 a         2581 c         1973 g         2918 t
ORIGIN      
        1 ataagcaacg aactccacta tgtcgaagcc tgtctgcggt atgacacact ttggctccat
       61 cagaaatggc ggttcatttt tgacaacgta ttcttctata tcgaaattgg cattgcactg
      121 gccgcttccc tcgtcgtacc tgccgtcgaa atatatgcag cgtgctgttg ccctgaacga
      181 gaaattccgt ccttccaggg cttttatctg gccttttgtc attgggacca cgaatatggc
      241 ttttgccatg ttgtcaaaaa tctggatatc ttcctgcggg gctgcctcgc cgttcagact
      301 gacctcccac ttctccctgt cttccctggt ataatacacg gctctggcca gcggatcctc
      361 catatgtttt tcatctttaa gtctcgcagt cacttcgact tttacgccga tcacctgctt
      421 ttcgaaacct tcagggttgt ttttcccata gtcgataaag ttgagttcat cccggttaat
      481 cacttttacc gagacttcca ccggggtatg ctctttctga tactttttaa tggtgaaccc
      541 catataaaac agtttgcctt tgctgtcaaa cctgaacatc ctgccttccc cgttgaactt
      601 ggccgagggc gcggactgga cattgagaaa gtggtggaaa tcccatgtac cgtcaggctt
      661 tcgttccggg gcagtatgct ttaaaccctc gtcggttacg ccattgcgaa tcttgaattc
      721 ggttatgcca ttattaatcc attctcgagt ccgcttttct atctctgaaa cattatttat
      781 tgcagcttta ttataaatac ttacacgcat aacaggttcg cctttgaaaa tgtcttttac
      841 atatttacta tcccagtaat ggtatatcca tgcaatatcc tcaagcggtg ttatcctctt
      901 tacgtcggga ggaaaattcg tattggcata cagattccct tcgatggtat atccgtcata
      961 cctgtactca ccacgtttat atcccggctt tttatagaaa ccgatgccgt tttcgttgag
     1021 agtatcctct ttgtaattct tcctgtagct ttcgttggtg tttgcctctt ctggcgtccc
     1081 gtacacgacg ataccttttc cgaaccaaat acggtagttg aaaggccgtc ccttcgcaac
     1141 gggcgtgact ccgtatggaa gtccagatag tttctcaaac agttctggat tgtcgaaata
     1201 tgtttcccac ttgcaacttg tttcataata ccgcgtatca tccattgcag ttgtaaaatt
     1261 tggcagcagc aaggacaaaa taagtataca tatgaaaaat ttctttttca tagcaaccag
     1321 ctacctctct gttttgaatt gtcttttatt atttcaggct atggatcgaa agtgagaacc
     1381 cgttatcgcc accgacttta taaaagaaca tttcacggcc attataggtt atctcttcta
     1441 cacggtgctt ctggtcaccg cagactgcat attccagata tctatcatat tccgccattg
     1501 ctttatttgc ttcgtctttg aaccagaact taaacatttc gtatacgatg tctcgatgaa
     1561 gccgctgtac agcttcagca tcatacagaa ctatgtgata tgggttatcc attatgcttt
     1621 catcgtctat cgtgttaaaa taaattgcca tttggatatt aagaatttta ttttccttga
     1681 acttctcctc actctcataa cagtcataga atatattatg tcctacttcg ctgaaccctg
     1741 aaacgacaaa tccttttgat ttttgcccaa catttctgaa tgcgttcgca gcatcaatta
     1801 tctctttttt agatgggggc catgctgtta caacagatcc atcataattc acacatttaa
     1861 atacctcatc ctctgccgga gtgaacggca cacgcgaggc gttatccagg tatctaatca
     1921 tgatggttgc cgcttccgcg cgcgtcagcg tgtcagccgg cctgaaccag cctccggccc
     1981 ccctgatgag tccgagctca taggactgca gcacatattg cttgttttca tcctttattt
     2041 ttgcataatc ctttatcttg gaccgcacaa gattatccgg attgttataa gggtcattgt
     2101 aggggaactc ttctttcagc agagttgcct ttactatgat ccttgttgcc tcttcacggg
     2161 taatgggacg cctgtaatcg gaaaactcgc ctttcgcgat cagcttttgc tgaagtgctg
     2221 tgtcaatata attctgagcc cagtatttcg tgttctcacc aggggcaaat cccatcgacc
     2281 tcacagtcat cttgagaaac tgatcgacct gcaccggatc gtcaggatga aacatgccgt
     2341 tatctccatt gataatgcca agcagcgaca gcatcccgac ctctttgcgg ctccagtgag
     2401 actgcatgtc cccatacgag tccagcaggt tttctatctc ttcgtctttt aattctgcaa
     2461 gcttttcctg cagcggcttt atcctgaagg ctgtttcatt ttcatacgtt ccttcggatt
     2521 caccggcata tgcgcgcgtc ggcgggaaca atgccaaaat acctaataac atggccatca
     2581 gaattatttc agtacaaatc cttataacat gcctgccatg ccatacaaac cttctgccat
     2641 acatatttcg gcctccttgt cattatcctg ttttacaggt ttattctcct gcctgcagct
     2701 aaaaataaac tcacagtttg ccagcctcag gatgtcattg tttttcagca gcatctcctt
     2761 attgcactca atccgtttgc catttatata agtgccattc atggaattca gatccttgac
     2821 ataacagcat ccattccttc gtattaaaac ggcatgcagc ttacctacgg cattgctgta
     2881 cagtatatgg tccacatgac cggcaagtct gccaacgata aattcgtccc tgtcgatcac
     2941 tatattcttc tctcccggct gtccgttcat atttatcagt acaaattcgc tttcattttg
     3001 catttctagg ctttcggtct tctgagaaaa acaagttact cctgactccg gaggcaatgc
     3061 cgcatctttc gctttcagat cagtccggat ttcttcggcg ctttcgcctt gcggtatact
     3121 aatatcattc cttgcggctg ccttttccgc aattgcattc ttccgactat cggtctcaca
     3181 atctgccgga ctctttcgca tatgacctgt cgtctgtata aatgcatttt gcgccattgc
     3241 ttcgcctggt attgcatccg gttcgccgtc cttatccggc ctggtactga ctatatccct
     3301 gatactgaac agtttcattg cctgcagttt cctgaagatc agcacttcaa tcgcaagaat
     3361 tatcagcgcg acagcaatat aattcactgt ggcatcattc cccgtaccgg caatgatccc
     3421 gcggcacata tatatcgcta tacccattgc aagctgcagc agcaccgcaa taacaactgc
     3481 aggcgatggt tttttcttcc ttatattctc cattgcttcc ttcttttcct ctttcttata
     3541 tttttctcta ttttcttcgg ccatttttat gcatatttcc ggctcgtacg gcggtttttg
     3601 cttttctctg atgtccggac tgtacagcaa ttcgtttaca agtgtaatca atcccctgat
     3661 gctgaatgtt tcgcttttca cctccgataa tatacgctgg acgagattac cgctgctaaa
     3721 accttcttcg tttatatgct gcatcagcaa ttcagaaacc agtgtctgca atgacccggc
     3781 atcatcatgc cggaattctg ccggtatata cacggtcatg acatccagcg tcccgggatc
     3841 gatatatata aattccggtt tcatcaggaa gttggacgga tacagcagat acccggctga
     3901 attgcttata caggccgtta tattcagaat cagcctgagg aaagcctccc tgctgagctt
     3961 attccttttc aggtatatcg ataacggtat ccgggaggtt atattatagt aaaagtagtt
     4021 ggcgtcctct ttctttacta tttccatcgg gatgacgtat tcgatatcat tgttttcgaa
     4081 catgccggcc tgatacccta tgatgttccc ggcacacctg attacaagaa aactggacgc
     4141 agcatcgctt tcatatataa actccagttc cttaactgct tctgcagcaa ccccggacat
     4201 agccgacttc ctccaaaaca tttcttttag tatgcagccg tcaaatgctg ctctctcaat
     4261 tgatctggtt gttttgtctg ataagctggc tgtttacatc gtttgtgaca gaatcactag
     4321 caaagatgct tttcatgatg tcagtaacaa acttgataat agcatctctg aatatgagcg
     4381 ctatacctac aagaacagcg ataataacga ctatttccac tgttccgagt ccatcctcct
     4441 cgctcaggaa gctttttacc agtttgttca tcataccacc tccgattctc atatatttct
     4501 cagggccagg actgcaggca tcccgactat gagcagtatt gcaatgaaca tcagcatcat
     4561 cggcagcagc agcttggtcg atgcttcttc cccgaacttc tttgctgtat ttttccgcat
     4621 ctcccaacat tcatctgaca ggagcctcag tacagggaca agctctgaat tgcctttcct
     4681 gatgttttgc agtactacgg tcacgaagcg tgttactgca gggacatggc acctttttgc
     4741 aaactcctca tatgctttat gttcggatac tcctgacctg acagcctgta ccgtgatgca
     4801 tagctcttta tagagcggag tctcccgctg agagtccatt gcagccttct cccatgcttt
     4861 cgatacagtc agcccggcat tgacaagcaa cgtcagttta ttgacaaagt ccgggaactc
     4921 gagctggatc gcctgacgtc ttttcctgac tttctcatgc agttcacggt cgctgagcac
     4981 aacagctgct acaaccaaca gaacgcagaa aaacagataa ccgccgtcga tatcggcaaa
     5041 aatccccaca aaaccggcaa acatcaatgc cagaagtacc agcgatattt tgttccccca
     5101 atgtacacgg agcatcagca gggctttcct gtgcccataa agttcagcaa ctgccatggc
     5161 cagcttcctg tcatatcctg tgttgtagct gtgcttgaaa gtgtccagaa taagcatgct
     5221 taccggaagg agccctttca gcggaaactc tttcttgtcc agcacgcata tgatatccgc
     5281 atacctgccc cttgataaaa agtacaatat taaataaata atggctaaaa cggcgaaaaa
     5341 aacagccatg gcagacctct ccgcaatatc atctgatctt tcgtcagtgt ttttcgcatc
     5401 atagcgattt ttcattggtg atgttaagaa ttattatggt atggtataga tgtcttactg
     5461 catcttttta cgcatatttc tgcaaaaaat ttccctgtat tttgagcaag tccgactaaa
     5521 acgtctgctg agccggtcaa acctttatat cattcagctt cgctgagatg aaccatgcca
     5581 acaccatcat tgccagggca atggtcatcg tcagccgccc ggcagctgta ttgaaaatcg
     5641 ggtacatata gtccggcgca ctggctgaaa gcagcgctat cataaacacc ggaacggcat
     5701 tcagcacctt ctgttcaaat ctgcgctcag ccagcagcat atcaatttcc tggccgactt
     5761 ctatcctgtc gcttatgatc gatgaagcgt ttcttataat ctgggctata ttcccgcctg
     5821 tcctcttgct tatactgagg acattgacaa agttgtctaa atcttcaagt cctgcacggc
     5881 ctgcaaattc agtcaatgcg gattcaagcg tctcattggt atcaagtctg ctgattatgt
     5941 tcctgatttc agcaagtata tatgtggacg gctccggata aagcacggag agatcctcca
     6001 gcgccgctct gaatgcggat tcgatggatt tgcccgcaga aacggatgaa gccagggaat
     6061 acagcaggtc cttaaactgg atatttagtt ccttcttcct tcgcttaacc aggtcctttg
     6121 cctttattcc agggtacagc agcgcaagcg gtgtaagaag gacagatatg agtacgtttc
     6181 tgtagaacag aaaacctgca gcaaaaatta ctgctgccgc tgccaaaatg taaagcatct
     6241 tttgctttgt ccccataaca tatacgttgt aatccgtcat tgcataccac cgcccagtcg
     6301 acaacggata cccgccattt taagcttgaa tgtcctggta agcatgtttc cggtccactt
     6361 gaggcagccc gttacgggtt ggctttcggc tgcctgaacg cccgctgtcc ggcagacata
     6421 cggccttatg gcgtatcccg ttcccgtatt gtccgcatct gtatccccga tgcatatgtc
     6481 caggccacca ctgctacacg gtactgcctc ctcaaataca aacagcggat tcagaatgat
     6541 ccgtccgttg ctgtaacccg ctacttcgct tatctcaagt actctcctgg acttgtccct
     6601 cagcctggca agatgcacca ttatgtctat ggctgaagcg atctgctgcc gtacagcatc
     6661 cagaggtatt ggggcagcgc tgacgaccat ggtttccagt ctcttaagca tgtcttcggc
     6721 agaattggca tgtccggtgg ataatgaccc gtcatgacct gtattcattg cctggagcat
     6781 atcaagagcc tcctcgcccc ttacctcgcc aactattatc ctctcaggcc tcatcctcag
     6841 tgacgtcctg ataaggtcgc ggatggtgat catcccttta ccttcgctgt ttgcgttcct
     6901 ggtctccagc ctgaccaggt tgtccacccc tatgatttga agctctgctg aatcttcgat
     6961 cgtgattata cgctcatccg gaggtatcct ggcagcaagt gcgttcagaa aggttgtttt
     7021 gccgctgcca gtgcctccac aaataaaaat gttgtatttt gcctttacaa gacagtccag
     7081 cacctccgcg gcctcaacgc tcaatgagcc tgcctccgtc agctgctcca gggtcatggg
     7141 tttatcggga aacttccgta tcgtcattat cggcccgttc agcgccactg gcttgagcac
     7201 aacattcacc cttgatccgt ccggcaaaac gaagtcgaca atcggagatg cctcgtttac
     7261 agtcctgttg gtccgtgaaa caatattatg tatcacatcc tccagtttct gctgatcggc
     7321 aaatgccgct tctgtcctgt atatgcggcc tgcgcgctct atgaatatct tgtccggccc
     7381 gttgaccatt atttcagtga tgcttttgtc gtccagcaaa ggctggagta tgtcgagcct
     7441 tctcatcgaa ttgtatacca tgtcggccag ttctcgtttt tgcgcgatat tgagccagga
     7501 aaccctggat ctgcggaaaa ccgctgctgt caccatttcc ataagctcgt cgtcgtcgat
     7561 atccctggtc ctgtccagtt cctgcgcgat ttccgtcctt atctcctctg ccagttttga
     7621 atctcccggc agcttgtccc tcttgtatcc gacattgcag atttcagcaa cttcaggcaa
     7681 cggatccacc tccatccgtg agaactcctg ccggcggcct gcgggccagt atctgctcca
     7741 caatgctgtt aaggctctta aggaatgaag tttcaccgat aaggctgccg gctgcagtgc
     7801 cgtcgtgcat tcgggaacaa tccgcaatca cagcggccgg cttgtatcca taaacggcgc
     7861 catattctgc aaatccggat gaaaaattca atacgggcaa aagcttgtcc gccaatgccg
     7921 ccttccatct gttttccaac agatcgaggc ctttgatgaa aataacggcc tttgactcta
     7981 tgacaggatc cagggcaagg accagaacaa tggcgtcgga atgcctcatc acttccgcat
     8041 ttacattgct tagacccgaa ggcaggtcaa caaaaaccat gtcatacaca caactcgacc
     8101 tgaactccct gataagccgt actgcatcct gttcatcaaa ctcattcatt tccagtatgc
     8161 tttcaggagg cttgtaaaaa tatacgccac tccttatatc cctgcacttt gcggactcca
     8221 gcctgagcca cagggagttg cttcctttca gatggtatat cacattggag aagctttgat
     8281 cagattcccc tctgaaaaaa ctgtctgttg agggagtaat ttcaagattc aggtaaaatg
     8341 tccgaaaccc catgcctgcg cacaatatgc tacagcctgc cgcaatactg cttttcccga
     8401 cgccgccggc aggtgaggca acactgacga tacacgtgtt cttgcgtccc tttaaaggat
     8461 gatccctgtc tgtacttgta gaatagatcc tcaatatctc agataccagc ctgtcgatgt
     8521 gctggtattt catgatcgaa acgggtgccg aatcacggca ttgaggtgcc tgcacgggac
     8581 tcccttcctc aagaagcagc ataagtccgc ggtcgtactg cccaattttt tcatcgtaca
     8641 tgaggctgtt cacaaggatg atatcagcag acttcggact gtcaaggaaa ccatgcagga
     8701 cctggggaga tgaaaaagat gtcagttcga atctcccggg gtagtttatg attaggtatt
     8761 ttccgaaatt ccgaatatat tcggcatcaa aatcagctat aacaagactg agcctcccca
     8821 aagcgctccc tccgcagctt acaattacaa ttatttttgt aaaaatttgg tttcaataat
     8881 attatatggg caggcccact ttctgtcaat tcctattttt caaaattttc caatgatatg
     8941 aacgctggat tcgcaaatta ctacagcagg cactattttg agaattttcc gataagctct
     9001 tcctgctctt ttctgacgaa gtttttgatc tggttgaaga cgatcggacc gttatgcctt
     9061 attttgagtc cgtattcgaa atatccgtta tgatcggctt tcctcactat ttccgcagtg
     9121 agagacagaa tgtctctcgc aaggtagata tcgaggttca gcttgtggcc tttgtaatgc
     9181 tcctcttttg cgtacagcat caacccgtaa tcgctgatat cctttaccag tgcgtaatat
     9241 ttcctgtttc ccgccgtacc ggctaccctg taatccgcgt acagagaaac aggaaaccgg
     9301 tcgtatgacc gcagcctgct tccttcgtcg ggcacatctt ccctgtaggt cagaaggctt
     9361 tcatctttat tataatcaac gatcctggct ccccttatga ctgctttctc atccagttca
     9421 taggccgtga ccaggggatc gccttccagg aaagttgcct tccggcactc cttcggaagc
     9481 tttacgacga tttgatcgtc aattattttc tcaaggatgc tgtcaaacac gttaatcatg
     9541 gaataatgcc tcatcgtaat gacactgcct tctgccggtc ccatgacaca cctccatgaa
     9601 ataaccctgt ttctgatttt attatattat aaatactaaa tatttggtag atttgcagta
     9661 ttccatataa ttccgcgccg cacctatatc tatcctcatt ctgtcaccga gtcggcatgc
     9721 cattaatata atgatactga cgacagcatc acaggccgga cgatagatct cacccggatc
     9781 tgtaacactt tgcacggaag aggaataaac taaattagcg gtataa
//
LOCUS       DAOSCC010000080         9693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4601_length_9693_cov_5.420315, whole genome shotgun sequence.
ACCESSION   DAOSCC010000080 DAOSCC010000000
VERSION     DAOSCC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_9693_cov_5.420315"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLG72_05785"
     CDS             <1..787
                     /locus_tag="PLG72_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600235.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HOJ80342.1"
                     /translation="GSLLISLGIGIAFGLVHGILVAYFDIQPFIVTLAGMFFARGMIT
                     IVSIAPRTASQEAFLKLKQARITLPGIGYYGKNGNFIPAQIEPGVVVAVAVVIIVAVL
                     LKWTRFGRNLYAIGGNSHSALTLGINVKRTKFFAYLIAGLLSGIGGYVYLLHTGAGDA
                     ANAAQAEMQAIASNIIGGTLLTGGVGNVIGTLFGMLSLKTITNIVIASGLTKPYYQSI
                     TTGLMLCFFILLQSLILAVREKGRMKLLPDWMRLRKTNRTESQ"
     gene            1196..1663
                     /locus_tag="PLG72_05790"
     CDS             1196..1663
                     /locus_tag="PLG72_05790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ80343.1"
                     /translation="MLRNMATVFLFNEDKLLMLKRSADKAFNPGVWSVIVGHIESHEW
                     KNPLATALRELSEETGFSESDLIDINFRYILIREARDEIRMQYVFVAKTDRRDFRDSN
                     EGELYWVPKEDILSKNMTVTIYNSLKHYLEHGNGDGVTVGVFDKDGRVVWSKI"
     gene            1779..1970
                     /locus_tag="PLG72_05795"
     CDS             1779..1970
                     /locus_tag="PLG72_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80344.1"
                     /translation="MRNGICAEDWLYKSRNIENISYQHDYEMVVDTLDKERYDWFMNA
                     MKTTYPNGHKWIRQSGHPG"
     gene            2060..2755
                     /locus_tag="PLG72_05800"
     CDS             2060..2755
                     /locus_tag="PLG72_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80345.1"
                     /translation="MDSGETRPNKKLVCISCLVLLCLVAAAVVYTVLSGSVPASLSED
                     EISELREKYPLYEEDPPLIEINKPKLEDLVKRADAFVLAEVVEASPDYTVKLIESSDT
                     PEGKLYEKWKDHGMQNTAPFMQCKVRIIENITGESSGKGEDIPGEIIIVMNAQHRGYA
                     PEPEPGMRIVTPVVKGTGGHEGKYFYSKYGFYYVTDDDHVLSAYVEDEDHKYSGKTLK
                     YLKKKLKKMSAEK"
     gene            2846..3703
                     /locus_tag="PLG72_05805"
     CDS             2846..3703
                     /locus_tag="PLG72_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ80346.1"
                     /translation="MLNELVSVNSKFVEIMEQQEGVLGAWYFGSVAHDTADGYSDIDI
                     VFLADEDSFGRIDERLDSLLSEACDEIVLRWDENFNSDAIKCYGYLLRYDGKLFLYDV
                     FLLNQGKIDDFMCRIHYTDLKPCDVIFDKNGSVQALIAKAVTGSRWHADIRRLVTTYW
                     FHVHMSAKYFVRQDFFKLEGILRTLMDTHASLLLSAYDRITWGGSANKLHYIPAEKQE
                     HLLLYGCVRDFALVRENMDKSMRWFDEDVREIGNEEIRAYNARLAEAIIPDWQDQTKF
                     LADGRACGT"
     gene            3936..4154
                     /locus_tag="PLG72_05810"
     CDS             3936..4154
                     /locus_tag="PLG72_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOJ80347.1"
                     /translation="MKFRKSIIIDLLILLVPLIAMIITTPYLPEKVPIQWGFSGEVTR
                     TVDRGYAFLLGLIPFVLYELFKFRYRNR"
     gene            4749..6032
                     /locus_tag="PLG72_05815"
     CDS             4749..6032
                     /locus_tag="PLG72_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ80348.1"
                     /translation="MKAGKQRTYDENLIREAGMRILPSAVIYGANASGKSNIIMSLAL
                     MQEIVTWGSVEASSPIMMNMELYPFAYNTEPEPILFQAEFINNGRHYLYSFEIIVKPF
                     QKDKRKIVSEQLWVSCGNKLVQVFERDSQRVDIKRDKKVLSLIQYNENLLAEFENKIN
                     INLDPAKLFLAHGFKTLISSDIADDVIDFFTSKLLVVNDFALRKLNISMAFSEMPKKS
                     VAVWNTLLDNFIKAADFGPQKLMLKPQKIEENRFEDMQLYSAYTPNGFEKPIMIPAEL
                     MESRGTLKLLDFAIPFITVFEEGTVFVLDEFDAIIHPELVKGIIALFNDQSFNKNGAQ
                     LIFSTHNPIYMSNRIFRRDQIKFVERDKDTYESTIHSLADFGSTEVRNDENYMINYFK
                     GKYSMLPYIDFTKFFNTPDNVKDPSTGRVPRKPGR"
     gene            6038..6658
                     /locus_tag="PLG72_05820"
     CDS             6038..6658
                     /locus_tag="PLG72_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HOJ80349.1"
                     /translation="MYRKSYFCVCAGQQEEMYLKHLAQLLKKPNERVVTFNTTVGSAE
                     LLRRNYTEYDKAVLFDHDFNEVEFERNIKTCVELDRESQKKRKRKGERIYHAYSNVNF
                     DLWLILHKEDYTRPVTRTDAYVDEVRRVYGLDPEADIKNRDVIKKILDQIELNDVKEA
                     IRRAERIRAGKLECDGFYVCSEVCYNNPDFSIHKFLKDVLEDCGEL"
     gene            6886..7137
                     /locus_tag="PLG72_05825"
     CDS             6886..7137
                     /locus_tag="PLG72_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80350.1"
                     /translation="MEFTIDPQGIWYHGSNKVFEVLEEGSTITQWKELAEAFSHKPSI
                     LAIDDDYSIFPLWILYAGLNFLWLWHSFSGMPTLRRLLL"
     gene            complement(7327..7803)
                     /locus_tag="PLG72_05830"
     CDS             complement(7327..7803)
                     /locus_tag="PLG72_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ80351.1"
                     /translation="MFNYAFIIRKAVESDAPAIHEIMQESFQKYMQDSNLTEPLEALT
                     ESVDDILSDIRTKEVFIALIDGIAVGSARVEILPDNTAYLSRFGVRPAYNNIGVGIAM
                     MSLIDKMLIEKGIKCVKLHTASKNKDLVRFYYGRGFYIDSTTNDRGYIRALLVKEY"
     gene            complement(8131..9060)
                     /gene="argF"
                     /locus_tag="PLG72_05835"
     CDS             complement(8131..9060)
                     /gene="argF"
                     /locus_tag="PLG72_05835"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ80352.1"
                     /translation="MKHLIDLIDLSLEDIEKLFALAEKLKKELKAGIPHPLLRGKTLG
                     MIFSKSSTRTRVSFEVGMYQLGGYAIFLSSNDIQLGRGETIHDTAKVLSHYIDGIMIR
                     TFKHSDVLDLAKYGTIPVINGLTDLMHPCQILSDLFTVYEKKGKLKGLKLAYIGDGNN
                     VANSLLQGCAITGMDISVASPRGYECDATIIEQAKHAAKKSGSKVVLTEDPEEAIKDA
                     DVVYTDTWISMGQEAEKEIRLKVFMPYQVDKKLFSLTKEDAMFLHCLPAYRGYEVTED
                     VIDGPNSNVFEEAENRLHMQKAIMVTLMADNQA"
     gene            complement(9279..>9693)
                     /locus_tag="PLG72_05840"
     CDS             complement(9279..>9693)
                     /locus_tag="PLG72_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ80353.1"
                     /translation="IPKGGNVLIATGEWDRDDCIALSKRFSKLGFNLMAVEDDYYCLN
                     GAGLLAELVPEDQILDYIKADKVSLVLSTHAKAGVPGKLGFAIRRTSMEYNIPCLTSL
                     DTANAILDVLEHLAECSEPGIYALDEYSRYEGKTC"
BASE COUNT         2640 a         2017 c         2442 g         2594 t
ORIGIN      
        1 gggctcgctg cttatttcat tgggcatagg catcgccttt gggcttgtcc atgggatatt
       61 ggtcgcttat ttcgacatac agccatttat cgtcactttg gcaggtatgt tctttgcaag
      121 gggcatgata accatagtaa gtatcgctcc ccgtacggca tcgcaggaag cgttccttaa
      181 acttaaacag gcacggatca cgctgccggg tatcggctat tatggaaaga acggcaattt
      241 catcccggcc cagatagagc ccggggtggt cgtggctgta gcagtagtga ttatagttgc
      301 tgtgcttctc aaatggacaa ggtttggacg caatctctat gccataggcg gaaacagcca
      361 tagcgcactg acccttggca ttaatgtcaa gcgcacaaag ttctttgcat acctgatcgc
      421 cggattgctg tccgggatcg gcggatatgt gtatctgctg catacggggg caggggacgc
      481 tgccaatgcc gcccaggcgg aaatgcaggc gatcgcctcc aatatcatag gcggaacgtt
      541 actgaccggc ggggtcggta atgttatcgg aacacttttc ggcatgcttt ctttgaagac
      601 cattacgaat atcgtaatcg cttcgggatt gacaaaaccg tattaccaaa gcatcacgac
      661 cggtcttatg ctgtgcttct ttatcctgct ccagagcctg atacttgccg tgcgtgaaaa
      721 gggccgcatg aagctgctgc cggattggat gagattgagg aagactaata gaactgaaag
      781 ccagtgacct gtctgaaagt gaacaaaata tctgatgcaa atttaccatg cgtcaggtat
      841 ttttttatta tgcctgccat aatcaaatac agtatttttt gcaaactgac tatgcggttt
      901 ttcttttact atagttacac ctggctccat attaaaaagt catttaattg acggaaaatc
      961 atcaactctt aataaaataa gttaatgatg gtttagtttt ccgaaatatg ttacaatctt
     1021 aaaaacaatg gctacaagta tataagagcg gaactgtcaa ggtattgaag ggacttttcg
     1081 tgctgcactg ccatgcagcc ggacaggact gatcgagata ctgatgagga aagccgaatg
     1141 atcctgttaa tgaatgcgcg gcggaagaaa tactaaacgg cggggtgcgg gaacgatgct
     1201 cagaaacatg gcgacagtgt ttctgtttaa tgaagacaag ctgctgatgt tgaaacgatc
     1261 agcggacaag gcgttcaacc cgggcgtctg gtcggtcatt gtcggccaca tagaaagcca
     1321 tgagtggaaa aatccattgg cgacggccct gcgggaattg tccgaagaaa cagggttctc
     1381 agaaagcgac cttattgata taaatttcag gtatatcctg attagggaag cgagagacga
     1441 gatccgcatg caatatgtct tcgtggcaaa aacggacagg cgcgatttcc gggacagcaa
     1501 tgaaggagaa ctgtactggg tcccgaaaga agatattctg tctaaaaaca tgacagtcac
     1561 gatatataat tcgctgaagc attacctgga gcatgggaac ggcgatggag ttacggttgg
     1621 ggtattcgac aaagacggca gggttgtctg gtcgaagata tgaagtgtgg cggtaaattg
     1681 gttataatgg gataaaaaga ttattgatta tttacaatgt accggggtgt atccattgaa
     1741 attactgcac gaattaaggg agcatctttc aggcatggat gcggaatggc atctgtgcgg
     1801 aggactggct ctacaaatcc cgcaacatcg aaaacatcag ctatcagcat gattacgaaa
     1861 tggtggtgga cacgctggat aaagaaagat acgattggtt catgaatgcc atgaaaacaa
     1921 catatccgaa tggccataaa tggatcaggc aaagcggaca tcccgggtga taccggatgg
     1981 ataaatatag aagtaaatga aaccatttag gcgcatcatt ggtctaaaat atgtaaaatg
     2041 taccaagggg ggcgtgtata tggattcggg tgaaaccagg ccaaacaaaa aacttgtatg
     2101 catctcatgc ctggtactgt tgtgccttgt cgctgcggcc gtggtttata ccgttttatc
     2161 aggttctgtg cccgcgtcct tgagtgaaga cgagatcagc gaactgagag agaagtatcc
     2221 tttatatgaa gaagacccgc cgcttataga aatcaataaa ccaaagctcg aagatttggt
     2281 taaaagggct gacgctttcg tcctggccga agtagtggaa gcatcgccgg attatactgt
     2341 taaactgatc gaaagcagtg atacgcctga gggaaaactc tacgaaaaat ggaaagatca
     2401 tggcatgcag aacaccgcgc cgtttatgca gtgcaaagtg cggatcatcg aaaacatcac
     2461 gggggaaagc tccggcaagg gggaggacat ccccggcgaa atcattatcg taatgaatgc
     2521 ccagcacagg ggctatgctc ctgagcctga accggggatg aggatagtca cgccggtcgt
     2581 aaaagggacc ggcggacatg aaggaaagta cttctattcc aagtacgggt tttattatgt
     2641 cactgatgat gatcatgtgc tgtctgccta tgtcgaggat gaggaccaca agtattccgg
     2701 caaaacctta aaatatttaa aaaagaaatt gaagaaaatg tccgcagaga agtaaaccgg
     2761 aaggctggat caggttggcg atgacgggct tttggtccgg tccgttatag tatcgggtcc
     2821 gcggatcagg aaaggaaacg tgcctatgtt gaatgagttg gtttcggtaa acagtaaatt
     2881 cgttgagatc atggaacagc aggaaggcgt gcttggcgcg tggtatttcg gctccgtggc
     2941 gcatgacacc gcagacgggt actcggatat cgacatcgtt tttctggcgg atgaggactc
     3001 cttcggacgg atcgatgaac ggctggattc cctgctttcg gaagcctgtg acgagatcgt
     3061 gctgcgctgg gacgagaact tcaacagcga tgcgatcaag tgttacggct acttgctcag
     3121 gtacgacggg aagctgttcc tgtatgatgt cttcctcctg aaccagggaa aaatcgacga
     3181 tttcatgtgc cgcatacact acaccgattt gaagccctgc gacgtcattt tcgacaaaaa
     3241 cggcagcgta caggctttga tagcgaaagc tgtgaccggc agccggtggc acgcggacat
     3301 caggcgcctt gttacgacct actggttcca tgttcatatg tcggcaaagt attttgtccg
     3361 ccaggatttc ttcaagcttg agggcatttt gcggaccctg atggataccc atgcttcgtt
     3421 gctgttgtcc gcgtatgaca ggataacatg gggaggcagt gcaaacaaac tgcattacat
     3481 acctgcggaa aagcaggaac atctgttact gtatggctgc gtcagggatt tcgcgctggt
     3541 gcgggagaac atggacaagt ccatgaggtg gttcgacgag gatgtgcgtg agatcggaaa
     3601 tgaagagatc cgggcatata atgcccgtct tgctgaagcg ataataccgg attggcagga
     3661 tcagacgaag tttttggcag atggacgtgc atgcgggaca tgacggatgt gcatgtgaaa
     3721 aatgatggct gcgcacatag gtaatatata cgcacatatg ggaaatacag ccgaatcagc
     3781 aatatcagcg gaacgattct gcgattgatt tgcagggcat gacttttgga aataccgatg
     3841 gcatgagaga aatggcctga tgtggcgtat tatgtataga aggttttcgg catgggttgg
     3901 tttaatatga tttgccagga agaagggctt attacatgaa attcaggaaa agcattataa
     3961 ttgacttgtt gattctgctt gtacctttga ttgcaatgat catcacgaca ccttatttgc
     4021 cggagaaagt gccgattcaa tggggtttca gcggtgaagt taccaggact gttgacaggg
     4081 gatatgcttt tttattgggt ttgataccgt ttgtacttta cgagcttttt aaattcaggt
     4141 acagaaacag atagaaggat cgttatgcca ggagagatcg ggccgaaaaa agtgcttgtt
     4201 gtttattgcc tgctatacga cgatatgaat aaaggacgtt tcaagagccg atgaattgat
     4261 gccttattat agatccggcg ttagtaaatt gatcaatagc tccgctgaat acatcaatca
     4321 gtgctagata cgagtatgtg gaagtttatc catacagtca cgagcaggag aaagattccc
     4381 acggcccgct tgtgatgtat gagaaattgg gttttgagag atgcgggctg tacatggatg
     4441 actgtattat tatgaggaag aagttatgat gaagccttga aataccagta catcctgcaa
     4501 atggacgaag gaacatgaga atatgatcct actgcggaac agatgataaa gactggagtg
     4561 tcctttcttt tttggagtat tttcgaaaac attgagaaaa tttctcgaat tagttgtaaa
     4621 aatcacagga aatgtatata atttataata gatattgaga tgtttttgca aatatcagga
     4681 ggttacttat gctgattagt tatagttttc gtaatttccg ttctttcagg aaaacggctg
     4741 tactggacat gaaagccgga aaacagcgca catatgatga gaatcttatc agggaggcag
     4801 gtatgcgtat tctgccctct gctgtgatat atggtgctaa tgccagtggg aaatcaaaca
     4861 tcattatgtc actggcatta atgcaggaga tagtgacatg gggttcagtg gaagcttcat
     4921 caccgatcat gatgaatatg gaactgtatc cgtttgctta taatactgaa cctgagccga
     4981 tcctgttcca ggctgaattt ataaataatg gacggcatta cctttattct tttgaaataa
     5041 ttgtaaagcc atttcaaaaa gataaaagaa agattgtttc agaacagctt tgggtaagct
     5101 gtggcaataa gctggtccag gtttttgaac gtgattcgca gcgtgttgat ataaagcggg
     5161 acaaaaaggt tctctcattg attcaatata atgaaaacct tcttgctgaa ttcgagaaca
     5221 agataaatat aaatcttgat cctgctaaac tgtttcttgc acatggattc aaaaccctaa
     5281 taagcagcga tatagcagat gacgttattg atttctttac aagcaagttg ttagtggtta
     5341 acgattttgc attgaggaaa ctgaatattt caatggcatt ttctgaaatg ccaaaaaaaa
     5401 gtgtggcggt ttggaacact ttattggaca attttataaa agcagcggat tttggacccc
     5461 agaaattgat gctaaagcct cagaaaatag aagaaaatcg ctttgaagat atgcaattat
     5521 attcggctta cacgccgaat ggattcgaaa aaccgatcat gatacccgct gaattgatgg
     5581 aatcgagagg aacgctgaaa ctacttgatt ttgccatacc atttataaca gttttcgaag
     5641 aaggtactgt tttcgtactt gacgagttcg atgcaataat ccatcctgaa cttgtcaaag
     5701 gcatcattgc tcttttcaat gaccaatcgt ttaacaaaaa tggagcgcag cttatattct
     5761 caactcataa cccgatttat atgagcaaca ggatattccg ccgcgaccag ataaaattcg
     5821 ttgagaggga taaagatacc tatgaaagca ccatacactc gctggctgat tttggatcaa
     5881 cggaagtaag gaacgatgag aactatatga tcaactactt caagggaaag tacagcatgc
     5941 ttccgtatat agactttaca aagtttttta atacgcccga caatgtgaag gatccatcga
     6001 ctggacgcgt accacggaag ccggggaggt aaagtggatg tatcgcaagt cgtatttctg
     6061 tgtctgtgcc ggccagcagg aggaaatgta cctaaagcac ctcgcacagt tgctcaagaa
     6121 gcccaatgaa cgtgttgtta catttaacac cactgtgggt tcagctgaat tgcttaggag
     6181 gaactatact gagtacgaca aagcggtctt gtttgatcat gacttcaatg aggtcgagtt
     6241 cgaacgaaat ataaaaactt gtgttgagct tgatagggaa agccaaaaga aaaggaagag
     6301 aaaaggggag agaatatacc acgcatacag taatgtgaac ttcgaccttt ggctcatact
     6361 tcacaaagaa gattatacca ggccggttac gcgaactgat gcatatgtgg atgaggtgcg
     6421 tagggtttac gggctggacc ctgaagcaga tataaaaaac agggacgtaa ttaaaaagat
     6481 acttgatcag attgaattaa acgatgtgaa ggaagccatc aggcgggcag aacggattag
     6541 agccggtaag ctagaatgtg atggtttcta tgtatgctct gaggtttgct acaacaatcc
     6601 ggacttttcc atccataaat ttctgaaaga cgttttagaa gattgcggag aactgtaaat
     6661 atgacgtaat gtatttaaat acatcatttc gcatttactt acgactatca tgggaagtag
     6721 tctttattag ctattcattg tgctgtaagt aaagcccgtc gaggaattat cggttttttc
     6781 ctgacgaatg cgcaacttca atataccgca aacctgcacg gatgatggaa ggaacacaac
     6841 tgcagaaata tgtgaataag atagcttgca tttgaggaga aggaaatgga atttacaatt
     6901 gacccgcagg ggatatggta tcatggctca aataaagtat ttgaagttct tgaagaaggc
     6961 agtacgatta cgcagtggaa agaacttgcg gaagcgtttt cccataaacc gtcaatactt
     7021 gctatcgatg atgactattc aatttttcca ttgtggatcc tgtatgcagg actgaatttt
     7081 ctctggttat ggcattcgtt ttcgggaatg ccgaccttaa gaaggctgct gttataatgc
     7141 cgtatgggat aatcatttcc cttgcagcat ttctcatcat atcgctgttc ttctggagat
     7201 gcccggattg cggcaggcaa tttgagataa ggcatgggca atggacaaag tatctgtctg
     7261 cccatattgc gggacggttt tgcgtgaccg gcgaagagaa gctaaaggcc acgtgtaccc
     7321 tcgtcttcag tactccttca ccaacaatgc cctgatgtaa cccctgtcat tggtggtgga
     7381 gtcgatatag aagcccctgc cgtaatagaa cctgaccagg tccttgtttt tggatgccgt
     7441 gtggagcttc acacatttta tccctttttc gatgagcatc ttgtcaatga ggctcatcat
     7501 tgcaatgccg acgccgatgt tgttgtaggc gggccttacg ccgaatctgc tcagataagc
     7561 tgtattgtca ggcagtatct ccactcttgc actccctacg gcaataccgt cgatgagcgc
     7621 gatgaagact tccttcgtcc tgatgtcgct taagatatca tcaacggatt cagtcagggc
     7681 ttccaggggt tccgtcaggt tggagtcctg catatatttc tgaaaagact cctgcatgat
     7741 ctcgtgtatc gcaggagcgt cggattccac ggcttttctt attataaaag cataattgaa
     7801 catgttttca gtaaccacct ttgatctgta tcgtaaatga gcaatgccgc tgtgcgtgcc
     7861 ggaagcagcg acctgtagct acgcaggacc gtcagcatcc ggtgacagac cgactcatca
     7921 gtccgtcata tctttggcat tctgtagaac cgtccctcgc attcacaaag ggacagtacg
     7981 gcaaatcaga ctgccataat atcggcattt tgaagaagcg tccctcacat ttctcacagg
     8041 gacagcccgg taaataaagc tgtctctctt ctgcattctg aaggaacgtc cctcatatta
     8101 tgttatgtaa attttacaga atcgtccccg tcacgcctgg ttgtcggcca tgagcgtcac
     8161 catgatggcc ttttgcatgt gcagcctgtt ttccgcttct tcgaagacat tcgaattcgg
     8221 cccgtctatt acatcctcgg tgacctcgta tcccctgtac gcgggcaggc agtgcaggaa
     8281 catcgcgtct tctttcgtca gcgagaagag tttcttatcg acctggtacg gcatgaacac
     8341 cttgagcctt atttccttct cggcttcctg gcccatgctg atccaagtat cggtatatac
     8401 aacatcggcg tcctttattg cttcctccgg atcttctgtc agtaccacct tgctgcctga
     8461 ctttttggcg gcatgttttg cctgctcgat tatcgttgcg tcgcattcat atccgcgcgg
     8521 agaagccacg gaaatgtcca tccctgttat tgcgcagccc tggagcagcg aatttgccac
     8581 attgttgccg tcgcctatat atgcgagctt cagtcctttc agcttgccct tcttttcgta
     8641 gacggtaaac aggtccgaaa ggatctggca gggatgcatc aggtcagtga gaccgtttat
     8701 gaccgggatg gtgccgtact ttgcaaggtc cagcacgtcg ctgtgtttga acgtcctgat
     8761 catgatgccg tcgatatagt gagagagaac cttggcagta tcgtgtatgg tttcgccgcg
     8821 gccaagctgt atatcgttgg aactcaggaa aatggcatag ccgccgagct ggtacatgcc
     8881 gacctcaaac gatactcttg tcctggtaga ggacttcgag aatatcatgc cgagtgtctt
     8941 gccgcgaagc agggggtggg ggatcccagc ctttagttcc ttcttcagtt tttccgccag
     9001 tgcaaacagt ttttcgatgt cctccaggga caggtctata aggtcaataa ggtgcttcat
     9061 cgcaacaact ccttacatga acattttatc cggcagcctt aaaaatgaaa agaccggccc
     9121 tgtggtccgg ccaggcggtt gtgtggccgc tgtcccgaca ggtcctttcc gctatgctgt
     9181 ttcagcaata caatcaaccg gtcttttttt attttcggca tactttctgc ctgaccttaa
     9241 cagtatgcct tttatcggat cttaacaggg gtcttttctt aacaggtctt tccttcatac
     9301 cttgaatatt cgtccagcgc ataaatgccg ggctcgctgc attcagcgag gtgctcgagc
     9361 acgtcgagta tcgcgttggc cgtgtcaagg gacgtcaggc acggtatgtt gtattccatt
     9421 gaggtccttc tgatcgcgaa gcccagtttc cccggcactc cggcctttgc atgcgtgctc
     9481 aggacaagcg acaccttgtc tgccttgata tagtcgagga tctggtcttc cggcaccagt
     9541 tcggccagca ggcccgcacc gttgaggcag taatagtcgt cttcgactgc catcaggtta
     9601 aagccaagct tgctgaacct cttagacagc gcgatgcagt cgtccctgtc ccattcgcct
     9661 gtcgcaatga gaacgttgcc gcccttgggt atg
//
LOCUS       DAOSCC010000081         9640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4628_length_9640_cov_4.537298, whole genome shotgun sequence.
ACCESSION   DAOSCC010000081 DAOSCC010000000
VERSION     DAOSCC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4628_length_9640_cov_4.537298"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="hypB"
                     /locus_tag="PLG72_05845"
     CDS             <1..532
                     /gene="hypB"
                     /locus_tag="PLG72_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOJ80354.1"
                     /translation="LLENILPRLGTRLRTAVIEGDLATQNDALRIEKTGVTALQINTD
                     GGCHLDPEMIAKQLRRLDLDSLDLIIIENVGNLVCPASFDLGEDMRMVVLSVAEGEDK
                     PVKYPTAFLNADTVVITKTDLAPYVDADADVMKRYIAGINPKAVVFETAKKDGVYMAD
                     QLVDYIIDQAGRKKGV"
     gene            538..2790
                     /gene="hypF"
                     /locus_tag="PLG72_05850"
     CDS             538..2790
                     /gene="hypF"
                     /locus_tag="PLG72_05850"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOJ80355.1"
                     /translation="MVRYSLTIRGIVQGVGFRPFMFRLAKSLSIRGFVKNTSEGVYAE
                     IEGDESDCAEFIDELEQHPPALALIEDVEVQKLQLLGDPDFIIMESSCGVRNAMISPD
                     IGICDDCRADITDRNNRRYRYAFTNCTNCGPRFTIIRDIPYDRKNTSMAGFIQCEDCR
                     REYEDPLDRRFHAQPNACPVCGPKLSFYLGGKLADGDPCELFRKCINDGMIVAVKGIG
                     GYHLACDALNEDAVARLRRNKIRYDKPFAVMMRDTGTIRRFCDMDGTEEETLACARKP
                     IVLLKKKEGCTVAESVAPCNDRLGVMLPYTPLHCILMENFDALVMTSANISDRPMIYD
                     DSEAMDWLFGIADAVLTHNRQIFRRMDDSVCMAVAGKVRMIRRARGYAPEPLRLNGNR
                     SVILALGPQQKNTFCLAKGEDAFLSGHIGDLDDMDTVRAYESEIQSYMRIFEAKPDII
                     ACDMHPDYVSTRYADRFRNVLPVYEIQHHHAHFASVLAEHDIGENAVGLILDGTGYGK
                     DGTLWGGEALWGDTGTSERIGHMLYAPLLGGEAAIREPWRMALAMVYVACGKDTALEY
                     FEGYGDKAELLLRAYERNINSPPASGAGRLFDAAAVLAGTRTHITYEGQAAIEFEQAL
                     DDSADGSYGIDITWESGMMIFDWRQLIRDIVRDARSGCGAGVISARFHRAMVQLLADA
                     VSLARRQYGGNIVALSGGVFQNAYILGNAVSKLEKNGFIVYSNEKVPANDGGISYGQA
                     AAAAWLSRGK"
     gene            2898..3131
                     /locus_tag="PLG72_05855"
     CDS             2898..3131
                     /locus_tag="PLG72_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOJ80356.1"
                     /translation="MCLAVPGTIKQIKDGYALVDYGGVSRQASTRLFMEAKVGDRVLV
                     HAGFIIQILDKDDGDELEKLIEETMGFTDAEEI"
     gene            3115..4230
                     /gene="hypD"
                     /locus_tag="PLG72_05860"
     CDS             3115..4230
                     /gene="hypD"
                     /locus_tag="PLG72_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOJ80357.1"
                     /translation="MRKRYRIEDIKNRISEIAAGLGQITVMEVCGTHTANIHKYGIQD
                     LLPGNVRLVSGPGCPVCVTDQKDIAAALSLAERDDVIFACFGDMMRVTCGDRSLYSLY
                     ESGKDVRVVTSALDALQIARDNPDRQTVYFGIGFETTAPQTAALIEAADESGIRNLSV
                     LSVHKTMPQAIRQLLGNGSGINALMCPGHVATITGAEAFGFIPDELGLPAVIAGFEAY
                     DIMAALLRILYMLQNCEIKCVNMYPRAVTGQGNKEALSLIYKVFEPCDAVWRGLGEIK
                     GSGLGIRDRYKEFDAARRFDISADETMKAEGIKEAEGCICASILCGKNIPADCVNFGR
                     RCTPDRPLGPCMVSAEGSCAAAIRYGDTPQLGVFLYG"
     gene            4375..5373
                     /gene="hypE"
                     /locus_tag="PLG72_05865"
     CDS             4375..5373
                     /gene="hypE"
                     /locus_tag="PLG72_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOJ80358.1"
                     /translation="MEKKITLAHGSGGQAYRELVQDVFLPAFSNDMLKPLTDSAVCSA
                     GEKIAMTTDSYVIKPLFFPGGDIGRLCVCGTVNDLAVSGARPRYISVGMIIEAGFDME
                     TLVRIVGSIAETAKEAGVHVVTGDTKVVEGGGVDGIFINTAGIGVFSEDRPPLAQRIA
                     AGDRIIISGYMASHGMAIMAARENMGFDPPIESDVAPLAALADRVLRSGCEVNAMRDP
                     TRGGVAATLCEWVTEDTDVIIYNDSLPVRPDVSAACEILGMDPLYIANEGIMLLSVPG
                     RYADTVLSALRSADDGKNAAIIGEVRKGSGNVYAVTEYGTSRKILMPRGELLPRIC"
     gene            5646..7061
                     /locus_tag="PLG72_05870"
     CDS             5646..7061
                     /locus_tag="PLG72_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOJ80359.1"
                     /translation="MERYVLVVCIAVPLLGAFILPLLGRISARLRRTMVLAFVAGAFI
                     CAAFTLPSLFCGSPVYLHYELPLGMGFGFYADALAVFMAMTASLVAAVIILYSFGYME
                     GYENQNEYYMMVCLFIGAMMGLVFSTNLIFIYTFWEISAICCWRLIGFYREEVTVRYA
                     NKAFAITVAGALIMLAGFVGIYGETGTFDLAAMKGAHIPAWTVVLILFGILSKSATFP
                     LHSWLPDAGVAPTPVTSLLHAAVLVKIGVYAYVRLFVVTFEIDEVFTTAVPVIAAVSA
                     LISAGAAMRETNIKRVIAYSTISQLAFIFAGLSCGNETGVVGGMLYILTHSAAKAGLF
                     LCAGIVEHNVHTKDMTRMGGLCKNMPVTAVAFILCAFSVMGIPPFGGFFAKYLVIDGI
                     ISSGNIMLGIVFILGAVMTVLYLTRLFVKVFLGPQTFPDVKEGTPSMVVSAVVLAVLT
                     LALGVFISLPSGLASLIGRWT"
     gene            7063..8949
                     /locus_tag="PLG72_05875"
     CDS             7063..8949
                     /locus_tag="PLG72_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ80360.1"
                     /translation="MLSAISSFLNNGANIIWFVIVLPAVLGVAARLSGKRYAARLVIS
                     MLSGAVNLLFAMSLYLSEGFYVVIPFASHGLDIALNINGYSAMFLAFTAAGFLLVSLY
                     SVVFLKGESYAGLFMLYLNISLAMINGALLSDSLGIMLFFWEGLLCTLFGMLLINNRQ
                     DPKAAVKALVLSGTADLLLMLGIIITVYQAGTPYVSQMQKLPVTGIAGIGFVCMMLGA
                     VGKAGALPFHTWIPEAAGGAPTPFLAAFPGMLEKFLGIYLAVRIATDIYDLQPGSGMS
                     IAVMALGTLTIIVGVAMALIQKDMKRLLAYHAVSQVGYMVLGIGTALPVGIIGGIFHM
                     LNNTAYKSCLFMTAGSIAKQTGTTDLRKIGGLAKHMPVTAACFIISGLAIAGVPPFSG
                     FFSKELVFDAALESNVVFYAGALLGAFMTAVSFLKMGRAAFAGGLKLPEDANNSQEPG
                     LIRVRESGFGLLLPMCVLAVPSILFGVANTWPLDGLVSPALGYTESFSGWPHSAVLII
                     ISLAVLALAVCDHIYGSRKSGSAINAADHIHYAPVLRSIYTAAEKGWFDPYNWIIRIV
                     GAWSAFCMFIERGVTWFYDVAVPGAAKGAGNLLHRIDNGSLSLYLAAAVAGVGIVVVI
                     FLAV"
     gene            8967..>9640
                     /locus_tag="PLG72_05880"
     CDS             8967..>9640
                     /locus_tag="PLG72_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ80361.1"
                     /translation="MSAMAYIAVSFLVVPLVIIIIINLIQRDFAKKICIYLAGASAAI
                     QMASSAVEFFMLSANRLHEYRFTLLWNMEEDAEHFGVTSLSLVVMFCIGFVSFISALI
                     ALRTIDKRKIYYVNLLMTLVLGMNGMVIVTDLFSLYVFLEIVGISSFVLIALFRSETG
                     LEGAFKYLVMSALASIFILTGLSLIFMQTGSLLYSKIGSNILIAEDGTKQTLAYVALI
                     FLISGFA"
BASE COUNT         2208 a         2218 c         2878 g         2336 t
ORIGIN      
        1 tctcctggag aacatactgc ccagacttgg tactcgtttg cgtacggcag tgatagaagg
       61 ggatctcgct acgcagaacg atgcgctccg catagaaaaa accggcgtca ctgccttgca
      121 gatcaatacg gacgggggat gccacctcga tccagaaatg atagcaaaac aactgcgcag
      181 gctggatctc gattccctgg accttataat catagaaaat gtcggcaacc tggtctgtcc
      241 cgcatccttc gacctgggcg aggacatgcg catggtcgtt ctgagcgttg cggagggtga
      301 agataagccg gtcaaatatc cgaccgcgtt tttgaatgcg gatactgttg tgatcacaaa
      361 gacggacctg gcgccctacg tcgatgcgga tgccgacgtg atgaaaaggt atatcgccgg
      421 gataaatccc aaggcagtcg ttttcgaaac ggccaaaaaa gacggggtat atatggctga
      481 tcagcttgtt gactacatta ttgaccaggc cgggcgcaaa aagggcgtat gaggcctatg
      541 gtacgttatt cgcttacgat aagaggcata gtacagggag tcgggttccg gccgttcatg
      601 ttccggcttg caaaatcgct ctcgatccgc ggattcgtga aaaacacttc tgagggcgtg
      661 tatgccgaga tagaagggga tgagtccgac tgtgccgaat tcatcgatga attggaacag
      721 catcctccgg ccctggcgct gatcgaggat gtagaggtgc agaaactgca gctgctcggc
      781 gatccggact ttatcataat ggaaagcagc tgcggagtga ggaatgccat gatatcgccg
      841 gatatcggta tctgtgacga ctgcagggcg gatatcacgg acaggaacaa caggcggtac
      901 cgctatgctt ttaccaactg taccaactgc gggcctcgct tcacgataat ccgcgatatt
      961 ccgtacgacc gtaaaaacac gtcgatggct gggttcatcc aatgcgagga ctgccggcgt
     1021 gaatacgagg atccgctgga caggcggttc catgcacagc ccaatgcctg ccccgtgtgc
     1081 gggccgaaac tcagctttta cctcggcgga aagctggccg acggcgatcc ctgtgaattg
     1141 ttcagaaaat gtataaatga cggcatgata gttgccgtaa aagggatcgg cggataccac
     1201 cttgcctgtg acgccctgaa cgaagatgca gtcgccaggc tcaggagaaa caagatacgt
     1261 tatgacaaac cgtttgccgt gatgatgcgt gatacaggca cgatcaggcg cttttgcgat
     1321 atggatggga ctgaagagga aacactggcc tgtgccagga agccgatcgt actgctgaaa
     1381 aagaaggaag gctgcacggt cgcagagtct gtggcgcctt gcaatgaccg gctgggagtc
     1441 atgcttccct atacgccttt gcattgcata ttgatggaga acttcgatgc gctggtaatg
     1501 acaagcgcga acatatccga caggccaatg atatacgacg acagtgaggc catggactgg
     1561 ctttttggca ttgcggacgc ggtattgaca cacaacaggc agatattccg gaggatggat
     1621 gacagcgtct gcatggcggt ggccggcaag gtgcggatga taaggcgggc acgcggttat
     1681 gcgccggagc ctttgagact gaatggcaac aggagcgtta tactggccct gggaccgcag
     1741 caaaagaaca ctttttgcct ggcaaaaggc gaagatgcat ttttgagcgg ccatataggc
     1801 gatcttgacg atatggacac cgtccgtgcc tatgaatcgg agatacagtc ttatatgcgt
     1861 atatttgagg cgaagccgga cattattgcc tgcgatatgc acccggatta tgtttccacc
     1921 aggtatgcag accgcttcag gaatgtgctt cccgtatatg aaatacagca tcaccatgcg
     1981 cattttgcgt ccgtacttgc cgagcatgat attggagaaa atgcggtcgg gcttatactt
     2041 gacggcacgg gatacggaaa ggacggcacg ctgtggggag gagaagcgct gtggggggat
     2101 accgggacca gtgagcggat cggacatatg ctttatgctc ctctcctggg aggagaggct
     2161 gctatccgtg agccttggcg aatggcgctg gcaatggtgt atgttgcctg cgggaaagat
     2221 acggcactgg agtattttga aggatacggc gataaggcgg aactcctgct gcgcgcatat
     2281 gaacgaaata tcaactcgcc gccggcgtct ggcgcaggac ggctgttcga cgcagccgcg
     2341 gtcctcgcag ggaccaggac gcatataaca tatgaggggc aggcagcaat cgaatttgaa
     2401 caggcgttgg atgattctgc agacggaagc tatggtatcg atatcacgtg ggagtcaggc
     2461 atgatgatat tcgactggcg gcaactgatc cgcgatatag tgcgggatgc caggagcgga
     2521 tgcggtgccg gagtgatatc agccaggttc caccgtgcaa tggtgcagct gcttgccgat
     2581 gcggtttccc ttgccagaag gcagtatggc ggcaatatag tggcattgtc gggaggtgtg
     2641 ttccagaatg cgtatatcct gggcaatgcg gtcagcaaac ttgaaaagaa cgggtttata
     2701 gtctattcta acgaaaaagt gccggcgaat gacggcggga tatcatatgg ccaggctgcg
     2761 gccgcagcat ggttgagcag aggaaagtga ggagcgggaa gcaaatgccg ggatacaaaa
     2821 aaagtgtagc cccctggctt gtctctctta tgatatggca gtagtttcga agatgagttg
     2881 aatatggagt ggtgactatg tgtcttgcag taccgggaac gataaagcag ataaaggacg
     2941 gatacgcgct ggtcgactac ggcggcgttt ccagacaggc aagcacacgg ctgttcatgg
     3001 aagcaaaagt gggcgaccgt gtccttgtgc atgccgggtt cataatacag attcttgaca
     3061 aggacgatgg cgacgaactt gaaaaactga tagaggaaac tatgggtttt acagatgcgg
     3121 aagagatata ggatagagga tatcaaaaat cgcataagtg agatcgccgc cggactggga
     3181 cagatcactg tcatggaagt gtgcgggacg catactgcaa acatacacaa atacgggata
     3241 caggaccttc tgccgggaaa tgtaaggctg gtatcgggcc ccggctgtcc tgtgtgcgtg
     3301 acggaccaga aggatatcgc cgctgcactt tccctggctg agcgggatga tgtcatattt
     3361 gcctgctttg gcgatatgat gcgggtcacg tgcggggaca ggagccttta ctccctctat
     3421 gaaagcggta aggatgtccg ggtcgtgacc tcggctttgg atgctttgca gatcgcgcgt
     3481 gacaacccgg acaggcagac ggtatatttc gggatagggt tcgagaccac cgcgccgcag
     3541 accgcggcac tgatagaggc tgcggatgag agcggcatcc ggaacctcag cgtgctgagc
     3601 gtccataaga ccatgccgca ggctatcagg cagctcctgg gaaatggcag cggaataaat
     3661 gcgctgatgt gtcccggaca tgtggcgacg attaccggtg cagaggcatt tggttttata
     3721 ccggatgaac tcgggctgcc cgcagtcatt gcaggttttg aagcgtacga catcatggct
     3781 gcgcttcttc gcatcctgta catgctgcaa aactgcgaga taaaatgtgt gaatatgtac
     3841 ccgcgggcag tgaccgggca aggtaacaaa gaagccctgt cgctgatata taaagtgttc
     3901 gaaccgtgcg atgcagtctg gcgcggcctg ggggaaataa agggcagcgg gctcggaata
     3961 agagacaggt acaaagagtt cgacgcggcg cgcaggttcg atatcagcgc ggatgagaca
     4021 atgaaagcag agggtatcaa agaagcagag ggatgtattt gcgcaagcat cctgtgcggt
     4081 aaaaacatac cggccgactg cgtgaacttc ggacggaggt gtacgccgga caggccgctg
     4141 ggaccgtgca tggtatcggc ggagggaagc tgcgctgcgg cgatcaggta tggtgacact
     4201 cctcagctgg gggtattcct ttatggttga ggaatgtcac gcgtttgaat ggtgcggtga
     4261 cactcctcga aagaggtaaa gggacactcc tcagcgagga tgtattcctt tgtggcagag
     4321 gaatgtccct tggaggttaa ggagtgtcac gcggtgaatg gcaggtgatt tgatatggag
     4381 aaaaaaatta cgctggctca tggttctggc gggcaggcat acagggaact ggtacaggat
     4441 gtctttttgc ctgcattctc aaatgatatg ctcaagcccc ttacggattc tgctgtatgc
     4501 agcgccggtg agaagatcgc aatgactacg gacagttatg taataaaacc gctgtttttc
     4561 ccgggcgggg atatcgggag gctgtgtgtc tgcggtactg taaacgacct ggctgtcagc
     4621 ggggcacgcc ccaggtatat atccgtcggt atgattattg aagccgggtt cgatatggag
     4681 acgcttgtcc ggatcgtcgg gtccattgca gagacagcaa aagaagccgg tgttcatgtc
     4741 gtgaccggag acacgaaggt ggtcgaagga ggcggcgttg acggcatttt cataaatacg
     4801 gcaggcatag gcgtgttttc tgaggacagg ccgccgctgg cccaaaggat agcggcaggg
     4861 gacaggatca taataagcgg gtatatggca tcacacggga tggcgataat ggctgccagg
     4921 gaaaacatgg gctttgatcc gccaatagag agtgacgtgg ctccccttgc agcgcttgcg
     4981 gacagggtat tgcgaagcgg ctgcgaggta aacgccatgc gtgacccgac acgcggcggc
     5041 gtagcggcga ccctttgcga gtgggtcacg gaggatacag atgtgataat atataacgac
     5101 tcattgccgg tacgccctga cgtgtcggca gcatgtgaga tactggggat ggaccccctg
     5161 tatattgcca acgagggtat catgctttta tccgtgcctg gccggtatgc ggatactgtg
     5221 ctttcggccc tgcgctcggc agatgacggg aaaaatgctg ctataatagg agaagtcagg
     5281 aagggaagcg gcaacgtgta tgccgtgact gagtacggaa cgagcaggaa gatcctgatg
     5341 ccgcggggag aactgctgcc gaggatatgt tagaggtgcg gtgacaatcc tctgcaggga
     5401 gcattcctct atagatgagg aatgtcacgc gtttgaagaa tggtgcggtg acaatcctcg
     5461 aaagttgtaa aggaacactc ttccgcaagg gtgtatccct gctcgacagg gggatggctc
     5521 ccaggttacc ttattcttct tgactacata atatgtttgt attaaaatgc tacattataa
     5581 atgatagaac tgtataaaga atacgcattt tattgcgtgt ttgactgcca gggtgaaacg
     5641 gaattatgga aagatatgtg cttgttgtgt gcattgctgt gccgttactt ggagcgttta
     5701 ttctgcctct gctgggcagg ataagtgcca ggctcaggag gacaatggtg ttggcttttg
     5761 tggccggcgc ttttatttgt gcggcattta ctttgccaag ccttttttgc ggttcgccgg
     5821 tttatctgca ctatgagctg ccgcttggaa tgggctttgg cttttatgcc gatgcgctgg
     5881 ctgtttttat ggcaatgacg gcttctcttg tggctgccgt gatcatcctg tattctttcg
     5941 gatatatgga gggatatgag aaccagaacg aatattacat gatggtttgc ctgttcatag
     6001 gcgccatgat ggggctggtc ttttccacca atctcatatt catatacacg ttttgggaga
     6061 tatcggcgat atgctgctgg aggcttatcg gattctaccg tgaagaagtt accgttcgtt
     6121 atgccaacaa agcatttgcc ataaccgtgg ccggggcgct cattatgctg gcgggatttg
     6181 ttggcattta cggagagacc ggcacgttcg accttgcagc catgaagggt gcacacatac
     6241 cggcctggac agtcgtattg atattgttcg gtatactttc aaaatctgcg actttcccgc
     6301 tgcacagctg gctgcctgac gcaggagtcg cgcctacacc ggtgacatcg ctgctgcatg
     6361 cggccgtgct cgtaaaaatc ggtgtttatg catatgtgcg cctgtttgtc gtgacatttg
     6421 agatcgatga agtatttacg acggcggtac cggtcatcgc cgctgtaagc gccctgataa
     6481 gcgccggtgc ggcgatgcgt gaaaccaaca taaagcgtgt catcgcttac tcgaccatca
     6541 gccagcttgc gttcatcttt gcgggattgt cctgcggcaa tgaaacgggc gttgtcggag
     6601 ggatgctgta catcctgacg cacagtgccg caaaagcagg gttgttcctt tgcgcaggca
     6661 tcgtggagca taatgtgcac accaaggata tgaccaggat gggcggttta tgcaaaaata
     6721 tgccggtgac tgcggtcgcg ttcatactgt gtgctttttc cgtgatgggg ataccgcctt
     6781 tcggaggctt tttcgcgaaa taccttgtga tcgacggcat catttcatcc gggaacatca
     6841 tgcttggaat agtgttcata ctgggagccg tcatgaccgt actgtatctg acgaggctct
     6901 ttgtcaaggt gttcctggga ccgcagacat tcccggacgt aaaggaagga acgccttcaa
     6961 tggtggtgtc ggctgtggtg cttgccgtac tgacacttgc gctgggagtg tttatcagct
     7021 taccttccgg gcttgcatcg ctgataggga ggtggacata atatgttgag cgcgataagt
     7081 tcgtttctga acaacggtgc aaatataata tggttcgtga tagtattgcc tgccgtgctt
     7141 ggagttgccg cacggctttc cggcaaacgt tatgctgcaa ggctggtaat ttcgatgctg
     7201 tcaggagccg tcaacctgtt gtttgctatg agcctttacc tgtcagaagg tttttacgtg
     7261 gttattccgt ttgcatcgca tggactggac attgcgctca atatcaatgg atattcggca
     7321 atgttccttg cttttacggc cgcaggtttc ctgcttgtga gcctgtacag cgttgtattt
     7381 ctgaaagggg aaagctatgc aggccttttc atgctgtacc tgaacataag ccttgccatg
     7441 ataaacggcg cccttttgtc tgatagcctg ggcattatgc tctttttctg ggaaggcctt
     7501 ttgtgcaccc tcttcgggat gctgctcata aacaacaggc aggatccgaa ggcagcggta
     7561 aaagcgcttg tattgtcagg tactgcagac cttttgctga tgcttggcat aataatcacc
     7621 gtataccagg cagggacgcc ttatgtgtcc cagatgcaga aactgcctgt gaccggcata
     7681 gcggggatcg gtttcgtttg catgatgctc ggcgcggtcg gcaaggcagg cgctctgccg
     7741 ttccacacct ggatccccga agcggccggc ggcgctccca ctccgttttt ggcggctttc
     7801 cccggcatgc tcgagaaatt cctcgggata taccttgctg tccgcatcgc aactgacatt
     7861 tatgacctgc agccgggaag cggcatgagc atcgcagtta tggcccttgg cacactgacc
     7921 ataatcgttg gcgtggcaat ggcattgata cagaaggata tgaaacggct gcttgcgtac
     7981 catgcggtca gccaggtcgg atacatggtg cttggcatag gtacggcact tcctgtcggc
     8041 atcatcggcg gcatattcca tatgctcaac aataccgcat ataaaagctg tctgttcatg
     8101 actgccggca gtatcgcaaa acagacggga acgacggacc tccggaagat cggcgggctt
     8161 gcgaaacata tgcccgtaac tgcagcctgc tttattatca gcggacttgc aatagcaggg
     8221 gtaccgcctt tcagcgggtt cttctcaaaa gagcttgttt tcgacgcggc gctggagagc
     8281 aatgtcgtct tttatgccgg tgcgctcctc ggagcgttca tgacggccgt ttctttcctg
     8341 aaaatggggc gtgcggcttt tgcaggagga ctgaaactcc cggaggatgc gaataattcg
     8401 caggaaccgg gcttgatacg tgtgcgggaa tccggcttcg gcttgctttt gccgatgtgt
     8461 gttttagccg taccgagcat cctgtttgga gtggccaata cgtggccgct tgacggcctg
     8521 gtctcacctg cgctgggata tactgagagc ttttcgggat ggccgcattc ggcagtgctt
     8581 atcattatct cgctggccgt gcttgccctg gctgtatgcg accatattta cggaagcagg
     8641 aaatcaggca gcgcgatcaa tgctgccgac cacatacatt atgcgcctgt gctgagaagc
     8701 atatataccg ccgcggaaaa aggctggttc gatccgtata actggatcat ccgtatcgtg
     8761 ggtgcgtggt ctgcgttctg tatgttcata gagcgcggcg tcacctggtt ttacgatgtt
     8821 gctgttcccg gcgcggcaaa gggcgcgggc aatctgctgc accgcatcga taacggaagc
     8881 ctgtcgcttt atcttgctgc cgctgttgca ggtgtcggga tcgttgtggt catattcctt
     8941 gcagtttgaa gagggggctc cggataatga gtgctatggc ttatatcgcg gtatcgttcc
     9001 ttgttgtgcc gcttgtcata atcattataa tcaacctgat acaaagagat ttcgcaaaaa
     9061 agatctgcat ttaccttgcc ggcgcctctg cggccataca gatggcttct tcggccgtgg
     9121 aatttttcat gctttcagcc aaccgtctgc atgaatacag gttcacgctg ctctggaaca
     9181 tggaggagga cgcggaacat ttcggagtga cttctctttc gctagtggta atgttctgca
     9241 ttggtttcgt gtcgttcata tcggccctga tcgcattgag gaccatagac aagagaaaga
     9301 tatactatgt caatcttctt atgacattgg tccttggtat gaacgggatg gtgatcgtga
     9361 cagatctctt ttcgctctat gtattcctgg agatcgtcgg catatccagc tttgtcctga
     9421 tcgcactgtt ccggtcggag acaggacttg agggtgcgtt caagtacctg gtgatgtccg
     9481 cgctggcctc aatattcata ctgacagggc tgtcgctcat attcatgcag accggaagcc
     9541 tcctgtattc aaaaatcggt tcgaatatcc tcatcgcgga agacggtacg aagcagacac
     9601 tggcatatgt tgccctgatt tttttgattt ccgggtttgc
//
LOCUS       DAOSCC010000082         9621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4642_length_9621_cov_5.447627, whole genome shotgun sequence.
ACCESSION   DAOSCC010000082 DAOSCC010000000
VERSION     DAOSCC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_9621_cov_5.447627"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLG72_05885"
     CDS             <1..652
                     /locus_tag="PLG72_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ80362.1"
                     /translation="LDSQKRAHEIRTDVAARLRNAIEQSKAVENIKVLSEAILSIASR
                     TNLLALNAEIEASQAGSAGLGFTVVAGEIRNLAENSKKTANEIQKVTKLVLESVQALS
                     DSAEEVLEFLENNVVKDYDMLLAAGEQYNNDALMISEMVTNLSATTQQLSASMQTMTQ
                     AINDVAKASEEGAAETGDLAREAAEIVRRTNEVLEKTRKVNDSANRLLELVSIFRI"
     gene            complement(843..2162)
                     /locus_tag="PLG72_05890"
     CDS             complement(843..2162)
                     /locus_tag="PLG72_05890"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOJ80363.1"
                     /translation="MDYKKLILDRLLDRYEKSKAFTDGTSRRRILIRLNSHEFPEYDI
                     EKPFVRETFNSVITELASREIVGFEWLKHEQGNIIDKVWLIVPNVSRAYEEISRKPKR
                     SILDSILERAEQAARFIDPEMGEDPVDGSANGQVSGDISDKGGIAWAGNFMHDVISSI
                     KDRASAAGLLPENEEQSLAVIRAVEEICRLRGTECLERVFSLRCYGDSKYFEKFVRGR
                     LISLLKKYYIGIDEAAEMTDDDILAQAGIRQSPEYVEFRGAISVSLEGNVIDFSLFPY
                     GVSINADTVRRLEVVSMGTVKRILFIENKANYVEFTIANRDPDMMIVYLGGFYSPLKG
                     LFIRKVYEKASEHNVRFYHWGDIDLGGFLIFNRLKTNIIPKLEPYLMDRTALETMREH
                     AVRFDDKYAAKLAELLADDGYKVFHDVIGYMLENRIRLEQEVFLLSG"
     gene            complement(2152..5601)
                     /locus_tag="PLG72_05895"
     CDS             complement(2152..5601)
                     /locus_tag="PLG72_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOJ80364.1"
                     /translation="MKLLKKLLLIHWHFFSHELIEFDRINFLTGKNASGKTTLIDALQ
                     LLLLGDANGRNFFNKAANERSSRSLKGYLRCELGDDGGTGFRYLREGRFTSYIACEFY
                     DDQKKSSFTAGVVFDCYEDGTDEHHFFILDSELPENHFIVDGVPMTYKDLRTFVNKNY
                     KRGKFDFPASDRSFQDILKGKFGGLKNKYFSLLKKAVSFTPITDIETFITEYVCDVKS
                     PVDISLMQDNIRSYKRLEFEADIMEERVRRLSEISEKYGSYLEESQRFEIQSYIIDRA
                     KLQVSLDKIDHLGREIAGLDSSIAEEKNRLTGINLELEEEKKKRDGLLEDKHRSDINN
                     KLEQLRKQKNDLIKEVGRLEGNVDEIIQRLRRYALEWRKAANKCEPLREMPDCDLKMQ
                     FSNELEVIAEYSEKTAGYAKHLIDIDAQTVDVIMNEDFGTISDDLGRFKESAQVLRAS
                     VGKKRNEYIKELDRKEMTLRELEKGIKDYDPKLILLRDEIKRELSMKYGKDIHVHILS
                     DLLEVRDERWRNAIEAYLHTQKFYLIIEPRYFVDALKIYDRLKFSMGFYDWGIVDTGK
                     LEEKQPVCEKGSLAEEVITADHYARLFVDFVMGRVIKCDTVEELRNHDRAITDTCMLY
                     QGYVARQLHPDRWKHPYIGRNAVEKQIEGVKQAIGLIMTEISACDICTQAVGAAADMG
                     VASSNEAESYIKTIQEAAVLPTLRARLAEVIEEEGKIDISWLIGIDKKIEESEKRIKN
                     LEQQKEECVSNIARYEGEKQDIASNRIPQEQNNAAESRKAIDDRYDSQWISSTGEPRF
                     MKELGMRSSAREVADNFSSQLERTRSQKAKKRDDLETARAEYNKVYQAPHDIKREDNA
                     AYDKELEELQGIKLPEYRKKIHDAKEKAFQQFQDDFLAKLKSNIDMVRNQIDELNSAL
                     KESVWGTERYRFTMAPRQEYVRYYNMITDEMLLEGYNIASQIFRDKHRDAIDELFRQI
                     IDVESELNADARAELEKNIKRFTDYRTYLQFDLEVTDTEGRTQRLSKTLRKKSGGETQ
                     TPFYIAVLASFAQLYRIQDPSFNRIRLIMFDEAFSKMDSERIQESIRLLRRFGFQCIL
                     SAPSEKVGDIAPLVDRNLCVIRNGNTAAVRAFDARKLIDESAGEYIAAASEQDQAGGR
                     YGL"
     gene            complement(5598..6269)
                     /locus_tag="PLG72_05900"
     CDS             complement(5598..6269)
                     /locus_tag="PLG72_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOJ80365.1"
                     /translation="MWVEGFEKMSNSEKEEMKRLLNVLLSKTYIVRDVYDTKEMMMKI
                     NPDYRFLERHFEIVSDYLSYSGWDVSKDNQYGVISIDNIYEYNKVKLDRFTTMTLYTI
                     RLIYEEERERVTLRNEIMTTTGQIFHKMITLNVLKRKPADREMTESLRVLAEHNVIQK
                     ISGNWADADARILILPSILFIVTNEKISRLHELLAEDPQQPETEAGTGDTDEPDIIRG
                     EAEAG"
     gene            complement(6259..7737)
                     /locus_tag="PLG72_05905"
     CDS             complement(6259..7737)
                     /locus_tag="PLG72_05905"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOJ80366.1"
                     /translation="MKLFDVLPQNLFSILACKNKSLYTEALFVLRKAFRQEMTIAKSD
                     LVSMLIANLDEALLTIDMDAEKDEFGLEDDDAASGQSLSATAHYILRRLRDTGWIEME
                     YQPDSFEEIITLPDYTIKLVNTLYSFTDEAVREYNSFVYSTYSALRTADTDRDDFMYN
                     ALLTAHENTVKLVDELKSLHNNIRRYHQALNTYVTANEVLKGHFDEYKSLIMDHIYHP
                     LKTLDSVPRFKIPILNIISGWLSDTELRKKIAEQAMQRGKYRSLDEAMDDIIRKMNEI
                     NDLYEGMDAMLDEVDRKNSAYTRASMEKMYYLLNTDRSIKGKLVDIITAAAPAFSMAP
                     GRKLPDRGGSPDTEYLIDMLADGVVLYRQGYIDDRSLYTRANKSSRHDDEKPLKIDLP
                     GEDIGEAVIQNFIKNVKKLYSRQKVMAFVDRLMAGREILNSSEIRLENDDEFIMLILA
                     VIDSSEKNRNYRVEFTDGYVECGGYRIPGMRFIRKGTVRNVG"
     gene            complement(7975..8349)
                     /locus_tag="PLG72_05910"
     CDS             complement(7975..8349)
                     /locus_tag="PLG72_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOJ80367.1"
                     /translation="MGTYKVFVYGTLLKGEENHNAYLSGSRFVCCADLPGYGLYNVDV
                     YPGIVPEGGERVKGEVYEVDRETLRRLDDLEDEGDLYTRREVTVETAEGPMEVLVYVW
                     NGDVDADRKVDYTKQPWRGLGI"
     gene            complement(8965..9597)
                     /locus_tag="PLG72_05915"
     CDS             complement(8965..9597)
                     /locus_tag="PLG72_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80368.1"
                     /translation="MRKLAVVLSVLSFILSVIIIFITVNKHVFVDFLSDRYPDAAFKI
                     ESVGIDLANMEITARVCDSEGIESRLRKKGSIVYSDYEQDKAKSLIRNELTETLRKSE
                     LLDYIEHIDVRILEKVEYAEIDGSLNPAIYLTVEYNDAVSGKRDFAQKSYNVISVIKE
                     AKYNNIGTYVFLQSTEHDAMSLTVYSEDSEADFDALLGKIEVVKEKENVQ"
BASE COUNT         2291 a         2508 c         1929 g         2893 t
ORIGIN      
        1 ccttgattca cagaagaggg cacatgagat ccgtaccgac gttgcagcaa ggctgaggaa
       61 cgcaatagag cagtcaaaag ctgtggaaaa cataaaagtc ctgtccgaag cgatcctttc
      121 tattgcttcc aggacaaacc tgcttgcgct gaacgctgaa atcgaggctt cccaggcagg
      181 aagcgccggg ctcggtttca ccgtcgttgc cggcgagata aggaacctgg ctgaaaactc
      241 aaagaagacc gccaacgaga tacagaaagt gaccaaactt gtgctcgagt cggtgcaggc
      301 cctttcagac agtgccgaag aagtcctgga atttcttgaa aacaatgtgg taaaggatta
      361 tgacatgctt ctggcagcag gcgaacagta caataatgac gccctgatga tcagcgaaat
      421 ggtaacaaac ctgagcgcaa cgacacagca gctttccgca tcgatgcaga ccatgacgca
      481 ggctatcaac gacgtggcaa aggcatccga agagggcgcg gcagaaaccg gcgaccttgc
      541 ccgcgaagct gcggaaatag tcaggaggac gaacgaagtc ctggaaaaga ccaggaaagt
      601 aaacgacagc gccaacagac tgctggagct cgtttcgata ttcagaatat aggctcgcat
      661 tagctcttgg aataacaact cttgagaagt actggttcat ggggagtatt agtttacggg
      721 aactgcagac ataaatctca tgcagttccc tgctttgtcc ctccggcagg aagcattgtt
      781 ttaccgctta tagacttcac cgaaaaataa caacaaatca tatatactac caagctgaca
      841 ctttagccgg acagcagaaa gacttcctgc tccaatctga tccggttctc aagcatatat
      901 ccgataacat cgtggaaaac cttgtaaccg tcatcagcta ataattctgc cagttttgca
      961 gcatatttgt catcaaaccg gacggcatgc tccctcatgg tttcaagcgc tgtcctgtcc
     1021 atcaggtatg gctccagttt cggtataata ttggttttaa gtctattgaa gatcaagaat
     1081 ccgccaagat caatatcgcc ccagtgataa aacctgacat tgtgttctga agctttttca
     1141 tataccttcc tgataaaaag gcctttcagc gggctgtaaa accccccaag gtacacgatc
     1201 atcatatcag gatccctgtt tgcaattgta aactcaacat agtttgcttt attttcaata
     1261 aacaggatcc tctttacggt tcccatgctc acaacttcaa gccggcggac agtatctgca
     1321 ttgatggaaa cgccatacgg gaacaaggaa aaatctataa cattaccctc aaggctgaca
     1381 gatatggcac cccggaattc aacatattcg ggagactgcc gtataccggc ctgcgccagt
     1441 atatcgtcat cggtcatttc cgccgcctca tcaatcccga tgtaatactt tttcagcagg
     1501 ctgatcagcc ttccccttac aaatttttca aaatactttg aatcgccgta gcagcggaga
     1561 ctgaacaccc tttcgagaca ttccgtaccg cgcagcctgc atatctcttc gaccgctctt
     1621 ataacggcaa gcgactgttc ctcattctcc gggagcagcc ctgctgcaga agccctgtcc
     1681 tttattgagg atattacgtc atgcatgaag tttcctgccc aagctatgcc gcctttgtcc
     1741 gatatatcgc cgctgacctg tccgttcgcc gatccgtcaa ccggatcttc gcccatctcc
     1801 ggatcgataa atcgggcagc ttgctccgct ctttccagga tgctgtccag gatactcctt
     1861 ttcggttttc tcgatatttc ttcataagcc ctggaaacat tgggaacgat cagccaaacc
     1921 ttgtcgatta tgttcccctg ctcatgtttc agccattcga accctactat ctcccgggat
     1981 gccagttcgg ttatgacact gttgaacgtt tctcttacaa aaggcttttc tatatcatat
     2041 tcgggaaact catgcgaatt cagcctgatc agtatccttc tccttgaagt cccatcagta
     2101 aaagccttgc ttttttcata cctatccaat aacctgtcca ggataagctt tttataatcc
     2161 ataccgacct cctgcctggt cctgttccga tgcagccgca atgtattctc ctgctgattc
     2221 atctatcagt ttcctcgcat caaatgcccg gactgcagca gtgttcccgt ttctgatcac
     2281 acataggttc ctgtccacta aaggcgcaat atcgccgacc ttttcggaag gagccgagag
     2341 tatgcactgg aagccgaacc ttctcagcag cctgatgctc tcctgtatcc tctcgctgtc
     2401 cattttgctg aacgcttcat caaacatgat cagcctgatg cggttaaatg acgggtcctg
     2461 tatcctgtaa agctgcgcga acgaggccag cacggctata tagaaaggcg tctgggtctc
     2521 tcctcccgac tttttccgga gcgttttgga aagtctctgc gtcctgcctt ccgtatccgt
     2581 tacttccagg tcgaattgca ggtaagtcct gtaatccgta aaccgcttta tatttttctc
     2641 gagctccgcc ctggcgtcag cgtttaattc cgactctaca tcgatgatct gacggaagag
     2701 ttcatctatg gcatccctat gcttgtccct gaaaatttga gaggcaatgt tatagccttc
     2761 gagaagcatc tcgtcagtta tcatattata atacctgaca tattcctgtc tcggggccat
     2821 ggtaaagcgg taacgttcag ttccccacac gctttccttc agggcgctgt tcagttcatc
     2881 gatctggttc ctgaccatat ctatgttcga cttgagtttt gcaagaaaat catcctggaa
     2941 ctgctggaac gctttttcct ttgcatcatg gatcttcttc ctgtactctg ggagctttat
     3001 gccctgcagt tcttccagtt ctttatcata tgctgcatta tcctctcttt ttatatcatg
     3061 gggcgcctgg tataccttgt tgtattctgc cctggccgtc tccaggtcat ccctcttttt
     3121 agccttctgg ctccttgtcc tctccagctg cgaactgaaa ttgtccgcga cttccctggc
     3181 cgaactcctc attccgagtt ccttcatgaa cctgggttcg cctgtgctgc tgatccattg
     3241 tgaatcgtac ctgtcgtcga tcgcctttct gctttcggcg gcattgtttt gttcctgcgg
     3301 tatcctgttt gaagcgatgt cctgtttttc tccctcgtac ctcgctatat ttgatacaca
     3361 ttcttccttt tgctgttcaa ggttctttat ccttttctcc gattcctcga tctttttatc
     3421 tattccgatg agccacgata tgtcgatttt tccttcttcc tctataactt cagcaagcct
     3481 tgcccttaat gtcggaagta ccgcagcctc ttgtatcgtt tttatataag actctgcttc
     3541 attactgctg gcaacgccca tgtccgctgc agctccaact gcctgagtgc atatatcgca
     3601 ggctgatatt tccgtcatga tgagcccaat ggcctgcttt actccttcga tctgtttttc
     3661 gactgcattt ctgcctatat acggatgctt ccacctgtca gggtggagct gcctggctac
     3721 atagccctgg taaagcatac atgtatcagt tatcgccctg tcatgattgc gcagttcttc
     3781 caccgtatca catttgatga ccctgcccat aacgaaatcg acaaacagcc gggcataatg
     3841 atcagcagtg ataacctctt ccgcaaggct gcccttttcg cataccggct gtttttcctc
     3901 aagttttccg gtatcgacta tgccccagtc ataaaagccc atggaaaatt tcagcctgtc
     3961 atagattttc agcgcgtcta caaaataccg gggctcgata ataagataga atttctgggt
     4021 atgcagataa gcctcgattg cattacgcca gcgctcatcc ctgacttcca ggagatcaga
     4081 aaggatatgc acatggatgt ccttcccgta tttcattgac agttctctct taatctcatc
     4141 ccggagtaaa ataagctttg gatcataatc ctttattccc ttctcaagtt cccttaaagt
     4201 catttctttc cggtcaagtt cttttatata ttcatttctt ttcttcccga cggatgcgcg
     4261 gagcacttga gcagattctt tgaatctgcc taaatcgtcc gatatcgttc cgaagtcttc
     4321 attcatgata acatcaacag tttgcgcatc gatatcgatc aggtgctttg cgtagccggc
     4381 agtcttctca gaatactctg ctattacttc cagttcattt gaaaactgca tcttaagatc
     4441 gcagtcaggc atttccctga ggggctcaca tttattcgcc gcttttctcc actccagggc
     4501 atatcgcctg agcctttgta ttatttcatc gacgttgcct tcaagcctgc caacttcttt
     4561 tatcaggtca tttttctgct tcctgagttg ttccagctta ttgtttatgt ctgacctgtg
     4621 cttgtcttcc aggagcccgt cccttttctt tttctcctct tccaactcca ggttgatccc
     4681 tgtaagcctg tttttctcct ctgctatgga cgaatcaagt cctgcgattt ccctgccaag
     4741 atgatcgatc ttgtcgagcg atacctgcag ctttgccctg tcaataatat agctctggat
     4801 ctcgaacctc tggctttctt ccaggtatga cccgtacttt tcagaaattt cagacagtcg
     4861 cctgaccctt tcttccatta tgtccgcttc aaattccagg cgtttgtaac ttcggatatt
     4921 gtcctgcata agcgagatgt cgaccgggct ttttacatca catacatatt cggtgataaa
     4981 cgtctctata tccgtaatgg gagtaaatga gaccgccttt ttcaaaaggc tgaaatactt
     5041 gttcttcagc ccgccgaact tccctttgag tatatcctgg aaactcctgt cggatgccgg
     5101 gaaatcgaat ttccctcttt tataattttt gtttacaaat gtccgcagat ctttatatgt
     5161 catcgggacg ccgtcgacaa taaaatgatt ctccggcagc tctgagtcaa gtatgaagaa
     5221 atgatgttca tcggtcccgt cttcatagca gtcaaaaacg acgcccgcgg tgaaactcga
     5281 ctttttctgg tcatcataga attcacacgc aatataactt gtaaaacgtc cttccctgag
     5341 atagcggaaa cccgtacccc cgtcgtcgcc gagctcacat ctcaggtagc ctttcaggct
     5401 cctggatgaa cgctcgttgg ccgccttatt gaaaaagttc ctgccgttcg catcgcccag
     5461 caataaaagc tgcagcgcat ctatcagcgt tgttttcccg gaagcgtttt taccggtcag
     5521 gaagttgatc ctgtcaaatt ctataagctc atggctgaaa aaatgccaat ggatcaggag
     5581 aagctttttt agaagtttca tccggcctct gcctcccccc ttataatatc cggttcgtcc
     5641 gtatccccgg tcccggcctc cgtctctggc tgctggggat cttccgcgag cagttcatgt
     5701 aacctgctta tcttttcatt tgtcacaata aagagaattg atgggaggat aaggatacgt
     5761 gcatctgcat ccgcccagtt tcctgatatc ttctggatga cgttgtgctc cgccaggacg
     5821 cgcaggcttt ccgtcatttc acggtcggcc ggcttccgtt tcaaaacgtt cagtgtgatc
     5881 atcttgtgaa aaatctggcc cgtcgtggtc atgatctcgt ttctcagcgt gaccctctcc
     5941 cgttcttctt catatatcag ccgtatggta taaagggtca tcgttgtaaa acggtcaagc
     6001 ttcactttat tgtactcata aatgttatca atggagataa ccccatactg gttgtccttg
     6061 ctcacatccc agcccgaata cgacaggtaa tcagaaacaa tctcaaaatg cctttccagg
     6121 aacctgtaat ccgggttgat tttcatcatc atctcttttg tgtcatagac gtccctgact
     6181 atataagtct tagagagcag gacattaagc agccgcttca tttcttcctt ttcagaattg
     6241 ctcattttct cgaaaccctc aacccacatt ccgcacagtt cctttcctta tgaaccgcat
     6301 accgggtatc ctgtacccgc cgcattcaac atatccgtcc gtaaattcca ccctgtaatt
     6361 cctgtttttt tcactgctgt cgatcaccgc aagtatcagc atgatgaact catcatcgtt
     6421 ttcaagccgt atctcgctgc tgtttaatat ttctcttcct gccatcaggc ggtcgacaaa
     6481 agccattact ttttgccggc tgtacaattt cttcacgttt ttgataaagt tttgaattac
     6541 cgcttcgcca atatcttcac caggcagatc gatcttaagc ggtttctcat cgtcatggcg
     6601 gctgctttta ttagccctcg tgtacaaaga ccggtcatcg atatagccct gcctgtaaag
     6661 cacgacacca tcggcaagca tatctatcaa gtattcagta tcgggcgatc cccccctgtc
     6721 cgggagtttt ctgcctggcg ccattgaaaa tgccggtgct gctgcagtga ttatatctac
     6781 caacttgcct tttatgcttc tgtcggtatt cagcaaataa tacatttttt ccatggacgc
     6841 ccgggtatat gccgaattct ttctgtcgac ctcgtcgagc atcgcatcca ttccttcata
     6901 cagatcgttt atttcgttca tcttccgtat gatgtcgtcc atcgcttcat caagcgacct
     6961 gtatttcccg cgctgcattg cctgttccgc tatctttttc cttagttcgg tatcggatag
     7021 ccagccggat attatgttca atattgggat cttgaaacgc ggtaccgaat ccagggtctt
     7081 tagcgggtgg tatatgtgat ccatgatcag ggacttatat tcgtcaaaat gccctttcag
     7141 tacttcgtta gccgtaacat aagtattcag cgcctggtgg tatctcctga tattgttgtg
     7201 cagggacttc agttcatcga caagttttac ggtattttcg tgcgccgtga gtaatgcatt
     7261 gtacataaag tcatccctgt cggtgtcggc ggtccggaga gctgaatacg tggagtatac
     7321 aaaggagttg tactcgcgga cagcttcgtc ggtaaacgaa tataacgtat tgaccagctt
     7381 tatcgtatag tcaggaagag ttatgatttc ctcgaaagaa tccggctgat actccatttc
     7441 tatccatccc gtgtctctca gccttctcag tatatagtgg gcagtggctg aaaggctctg
     7501 gccgcttgca gcgtcatcat cttccaggcc aaattcgtct ttttccgcat ccatatcgat
     7561 agtaagcaac gcttcgtcca ggttcgcgat cagcatggac accaaatcgc ttttggcaat
     7621 ggtcatttcc tgcctgaacg ccttgcgcag tacaaaaaga gcctcggtgt aaaggctttt
     7681 gtttttgcaa gccaatatag agaacaaatt ctgcggcaaa acatcaaaca gtttcacgga
     7741 aaagaccacc cggttcggct atcaattttt tattttacgg catttcaggt ttcattttca
     7801 ttatacaggt cctgttcagc cttatcaatc ctaattttgc ctctgcttac attatgtgac
     7861 gtgattttat actattttac taatattagt atttatgtaa aaaatatttg atgttcggtg
     7921 taatttgcag gtcaaaccct atagccagca gcattcggaa aagtgaaaga tgtatcagat
     7981 acccagccct ctccagggtt gttttgtata gtccactttc cggtccgcat cgacatcgcc
     8041 gttccatacg tacacgagta cctccatcgg tccttccgcc gtttcgacgg tgacttccct
     8101 gcgggtatac aggtcgcctt cgtcttccag gtcatcaagc cgcctgagcg tttcccggtc
     8161 gacttcgtat acctcacctt tcacccgctc gcctccttca gggacgatgc cggggtaaac
     8221 gtccacattg taaagcccgt acccgggaag atccgcacag cagacaaacc tcgaacccga
     8281 caggtaagca ttgtggtttt cttcgccctt caaaagcgta ccatagacaa aaactttata
     8341 tgtgcccatt gctgcgattt cgtcctccct tgtaacctcc ttcgaaagtg tcaactgata
     8401 accggtactg tattatagtt aaccgttgtt ttgcgtcatc cgggcaacag gctgaataat
     8461 gcctgcccca gcccgctcag gttttatggc aggcaataat caaatatctt cccctggcat
     8521 attaggcatc ttttaaactg cgacccctcg catacactcg gtcagatgac tggcgtcaag
     8581 tattggtatg cttttagaat accggttctg aaaagataat gcaagcagtt ttgttgcctt
     8641 acttttgctc caggacacat cattgacaat tagccgtcca ggttccatat acttaacaat
     8701 aaatgcaatt tgtgttaaca taaatatagt tattggacat aaatgttaaa ttacaggagg
     8761 aaaataaaaa tgagtgaaca ggagaaactt gcatatttaa aggcattgat gtacattgca
     8821 ctatctgatg acaaggtcga tgatgatgag atccaatatt tcgacaggat tggtacagct
     8881 tatggcctct ctgtatatca aataaagtat cggcaaaaca tcattccgaa gcacatgccc
     8941 agccggtatt tatcgcagcc tggttcactg cacattttct ttttctttga ccacttctat
     9001 tttacccaat aaagcatcaa aatcagcttc tgaatcctcc gaatatactg tcaggctcat
     9061 ggcatcatgt tcagtacttt gcaggaatac atatgtacca atgttattgt attttgcttc
     9121 tttgataacg ctgatgacat tataactttt ctgtgcaaaa tcccttttgc cgctgactgc
     9181 gtcgttatac tcaaccgtga gatatattgc cggattcagg ctgccgtcaa tttcagcgta
     9241 ttccactttt tcaagtatcc ttacgtcaat gtgctctata taatccaaca gctcactttt
     9301 gcgcagcgtt tcggtcaatt cgttcctgat caggcttttt gccttatcct gttcgtagtc
     9361 gctgtaaaca atgcttcctt tttttctcaa tctagactct atcccttcgg aatcgcatac
     9421 acgggcggtt atctccatgt ttgccaaatc gatgccgacg gattctatct taaaagctgc
     9481 atccgggtac ctgtcgctta aaaaatcaac aaaaacatgt ttattaactg ttataaaaat
     9541 tatgatcacg gatagaataa acgacaatac gcttaataca actgccagtt ttctcataat
     9601 atgactccct caacagtcaa c
//
LOCUS       DAOSCC010000083         9591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4669_length_9591_cov_6.624895, whole genome shotgun sequence.
ACCESSION   DAOSCC010000083 DAOSCC010000000
VERSION     DAOSCC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_9591_cov_6.624895"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1276)
                     /locus_tag="PLG72_05920"
     CDS             complement(56..1276)
                     /locus_tag="PLG72_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOJ80369.1"
                     /translation="MATKNRMSLLEQLSKYVVEKDKDFLKEALTLLINALMDAEVTSM
                     IGAEKYERSENRNNHRNGYRQREWDTRLGTLQLSIPKLRHGSYFPSLLEPRKMSEKAL
                     LNVVQEAYVHGVSTRKVDELVESLGIKGMDKSEVSRISKQLDGFVTEFKNRILEGEYP
                     YLWLDATFPKVREGGRVCSMALVIAVGVNQSGEREILGFDVGMSEDGAFWEEFLRKLV
                     ARGLKGVKLVVSDAHEGLKSAIKKVLTGSTWQRCRVHFMRNVLSQVPKKCQGMVSSII
                     RTIFAQNDQESAREQLRHVVDELRGRFPKAMEILEAAEEDILAYMAFPREHWIQIHST
                     NPLERLNREIRRRTDVVCIFPNRDAVIRLVGTMLMEQNDEWQVGRRYFSLESMEKLTQ
                     ANGFSLAPIAILHK"
     gene            1617..2048
                     /locus_tag="PLG72_05925"
     CDS             1617..2048
                     /locus_tag="PLG72_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80370.1"
                     /translation="MKKYLLLCLSVLMVFQMNAYAVSFDNKSLPSGDLSVIKPQMTYI
                     IKASCNLTITADGQAQVESSIVGYIGVTGVEIKAELQQYKNGVWSSINSWTKSSNNYR
                     LNLAESTQVSKGYSYRVVATVTAYSGNDSETRSIVSDEDTY"
     gene            2684..>2977
                     /locus_tag="PLG72_05930"
     CDS             2684..>2977
                     /locus_tag="PLG72_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80371.1"
                     /translation="MCNLPDLVVNIAIGGCNCGNVPEDILKRQVDRVLYDTIACAQEF
                     NNPDYRFSGADIKVNLFSNPFMADAFWIDFADRGNMPLNIIVPNDEKSGEFRTL"
     assembly_gap    2978..2987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2988..3470
                     /locus_tag="PLG72_05935"
     CDS             <2988..3470
                     /locus_tag="PLG72_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80372.1"
                     /translation="TLIDKAGSVTNIGADSLRSAGSGSVMADWIADQADLSIFLWDGR
                     EEFNSREIWTLIQACKRNNIPSVWIDINSPESIYWTLDSYYDEYTKARLREYIAKLFC
                     EDGDAFDPPNEAKIPLWQLWDRLYGSFMKKYRAKVEPVPYICDKIMAKEPIENAVDEK
                     "
     gene            3573..4034
                     /locus_tag="PLG72_05940"
     CDS             3573..4034
                     /locus_tag="PLG72_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80373.1"
                     /translation="MPFIATVFISVGFYAESILGFIYQIPYSYISPLAVIAGIGFLIH
                     ALINYYMYDLSENPNVTSWHIRFIDNRFIAEVLRLTAHFAPFGIPVNHISSLNRFGNK
                     VSQRPHISGELRRITKSMEPADFVFDRHTSADLLENLTNMIDDQIAYHDRT"
     gene            4112..4471
                     /locus_tag="PLG72_05945"
     CDS             4112..4471
                     /locus_tag="PLG72_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80374.1"
                     /translation="MRGGLQFALVYAKTDIARNGINLISFVKSFANMLALMVPSWASY
                     FSLKLSLGNFEGLASNSRAMADGLAMMKKMLDDEKKRTDISFERIYAFSKDISRLMLG
                     EVVEWYSQLSSYKLTKL"
     gene            4495..5355
                     /locus_tag="PLG72_05950"
     CDS             4495..5355
                     /locus_tag="PLG72_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOJ80375.1"
                     /translation="MGRKYDVFISYRRQGGEQTARIICDRLMDSGYKVFFDVESLRPG
                     AFNTKLYSVIDECRDVIVVLSQNSLDRCKDENDWVRLEIAHALKAGKNVIPVFLRGFV
                     FPGTLPGDIEPLRYQNGLEASSEFFDAFIGKLKTFLKSKPTLLRRVSQNVVFKRTLPF
                     LLALLIVLGTGTGAYVVIRNRAQTFPGTREQKNITNEALSYIEKNLVLIDGAFGEVSA
                     AYKACGNYLASMDQAHYEEAVATINRAYGNINRLNFSQYALSGTALEKIDNTPINKAD
                     LVEVNAQNQT"
     gene            5397..5804
                     /locus_tag="PLG72_05955"
     CDS             5397..5804
                     /locus_tag="PLG72_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80376.1"
                     /translation="MLRFISLQLYDEAEKCAQIYQQKVQGDYPEAKEYVPAAIRFIRQ
                     ISSTGVDYGVIVCGYEPGKPRHEVYEIGDIIVAINGVMCLNLNKYSELVPKGQEYSAK
                     VLRPDAEGKLEFLELTIPPGQSRILLMNMTESE"
     gene            5869..6156
                     /locus_tag="PLG72_05960"
     CDS             5869..6156
                     /locus_tag="PLG72_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80377.1"
                     /translation="MIEKYIDSFFDELEVFDDLAKGRSNKAYLKGSIMEFLGDGNREN
                     AMNVYVSFFDSYRIVLEGESNQFIDLLDVLRNYEERRRPCLTGTGTTMCIP"
     gene            6153..7658
                     /locus_tag="PLG72_05965"
     CDS             6153..7658
                     /locus_tag="PLG72_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HOJ80378.1"
                     /translation="MNVFILGLCIYIRNGSFRKAFDKVGRNKEIYKYSYDTRHEEFLY
                     RWGIASLFHDVGYPVEITAKQIGKFVSFITDTVDGERRVKAGIDLDTFEDFNSIPEVI
                     PKEEFTKEFSRNVSESSRIDLLKPLDLIALKIHKDFHVDLETARKSLDRYFDAMKKSG
                     IADHGFFSAIIVLKWYGFLIQKCGYNPNYFFYPVVDCASAILLHNYYRNVLMKEPFRL
                     GKLSPELNPVAYLLILCDELQEWNRRAYGTIDRKRASAGNIKAAISNREISLTYISQN
                     GMLPDSFAAEKTELLDRVLDIGSIFPGGIHLSCEGNGDTPAGLQDEAAADDTGTDMIV
                     PRPLLENLEKLARMIHQEYNARQLERYPERKLEYPTWESLPDDLKYSNMRQARRILDK
                     LRLIGCTVMPKGSGSSRVSRFTDGEIELLAEAEHDFWVRERLSQGWKYGAKKDVDKKI
                     SPYLVPYEQLDEEAKQLDRDTVTNIIPLLESIDLEVCRISNGRMIAKEKGI"
     gene            complement(7834..9297)
                     /locus_tag="PLG72_05970"
     CDS             complement(7834..9297)
                     /locus_tag="PLG72_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOJ80379.1"
                     /translation="MQKARTVVGYLPDERPPFWKLLFYAIQQVIVMFPATVAVALITG
                     FQVSTTIFASGLATLCFILITGRQIPLYYGSSFSYLTAITGLMTSEAIKTMVSPGTLE
                     KLEAWKQGTTSILPAEAISYAQFGIIMSGLISIAAGFLVRAFGSAAVEKILPPSITGP
                     VAMIIGLTLAGTALSDAAPKPVVEGAAATVMNSNWVWVVSLVTLLSTILFSRYLKGFL
                     GQLPLLLGAAVGCITAGIIYAVGGADFNLFREIPKEALEASLWHAGPIAVPAFTLPKP
                     SLAAMVAIMPIAIATIPESTAHMYQLDVYVNDLAKKKGSSKKYNLAEKLDINLIGDGI
                     GDMISGLVGGPAGTNYGENISTMAITKVFSVSVLALAAVIAMVISFFTPLVQGIYSIP
                     LAVIGGLEVYLFGAIAAQGAAIMIEKKVDMFSPKNIAVIATIMVIGLGGNYAFGGNIP
                     FFGIEVPCIAGAAIFGILLNLILSIGEKKQAKEAEEE"
BASE COUNT         2636 a         2135 c         2301 g         2509 t
ORIGIN      
        1 ggatagtgtc ttgtcaagtg gtgtaaaatg atttctactt tatcttccct tcgtttcatt
       61 tatgtaagat agcaatcgga gctaatgaaa acccattagc ctgtgtaagc ttttccatag
      121 attctaaact gaagtatcgc cgccctacct gccattcatc gttctgctcc ataagcatag
      181 tacctacaag gcgaataacg gcatcacgat taggaaatat gcatacaaca tcagtccgac
      241 ggcgtatctc acgattaagc cgttccaatg gattcgtaga atgaatctgt atccaatgct
      301 cacggggaaa ggccatatac gccaatatat cctcttcggc tgcttcaagg atttccattg
      361 ctttcggaaa acgccccctc agttcatcta ccacatgtct tagctgttcc cttgcggatt
      421 cctgatcatt ttgtgcaaaa atggtgcgta tgattgagga gaccattcct tggcacttct
      481 tcggcacttg gcttaatacg ttccgcataa aatgtactcg gcatctttgc catgtgctcc
      541 cggttaaaac cttctttatg gcggatttta acccctcatg ggcatcactt acaactaact
      601 tcaccccttt aagtcccctt gctacaagct ttctcagaaa ctcttcccaa aaagctccgt
      661 cttcactcat gccgacatca aaacctagta tctctcgttc accggactga tttacaccta
      721 cggctattac aagtgccata ctgcatactc ttccaccttc acgaacctta ggaaaagtag
      781 catcaagcca aagataaggg tattctcctt cgagtatgcg gttcttgaac tcggtgacaa
      841 agccatccaa ttgcttgctg atcctggaaa cttcgctttt atccatccct ttaattccga
      901 gagattctac caactcatct acttttctag tgctgacacc gtgcacataa gcttcctgca
      961 ccacatttaa taaagctttt tctgacatct tccgaggctc taataaactt gggaaatagc
     1021 ttccatgcct caatttgggt atgcttaact gtaacgtacc aaggcgagta tcccattctc
     1081 tttgtcggta tccattacga tgattgttcc gattctcact tcgttcatac ttttccgcac
     1141 cgatcatcga cgtaacttcc gcatccatca gagcattgat taacagcgta agcgcttcct
     1201 ttaaaaaatc cttatctttt tcaacaacat acttgctaag ttgctctaaa agtgacattc
     1261 tatttttagt agccacggta tgacctcctt tactttatct tttttgtttg aatctaataa
     1321 agtaaaaatc atatcgtggt tactcttgtc aagcatactc cacgaaattt acaccactac
     1381 ttaagactat aaccctaaca catatcgcgt aacttaccaa attttagaac ctaatggtag
     1441 tgttatcaac ggtaagtctt ttagtaatga tatatcaaat gaaaaggtta cattagactc
     1501 attagggtat gatgagttta ttgcatatat tgcaggtgat ggcacgaata attataaggc
     1561 ttggggttca ttccattact aagtattaac tataataaac atggaggaat catataatga
     1621 aaaaatactt gttgctttgc ttgagtgtac tgatggtatt tcaaatgaat gcgtatgcag
     1681 tttcttttga caataagtca ttaccatcag gagatctctc cgttataaaa ccccaaatga
     1741 cttatataat aaaagctagt tgtaacttaa ctatcacggc tgatggacaa gcgcaagtgg
     1801 aaagcagtat tgttggttac attggtgtta ccggcgtaga gataaaagca gaattgcagc
     1861 aatataagaa tggagtttgg agttccatta atagttggac caaatcaagt aacaattaca
     1921 gattgaacct tgcggaatcc actcaagtat caaaaggtta ttcgtataga gttgtagcaa
     1981 cagtaactgc ttatagcgga aatgactctg aaaccagatc gatagtaagc gatgaagata
     2041 catactgata gaaacacttc cgatgctatt atgataacat cagttatttg ttaacgatta
     2101 tggcgcctga tagcttatat tggaaaaatg ctgagtagaa accaaggtgc caactaagat
     2161 caggcaaggc ggtgaaattg ttgaaaccgc ctgttttttc tgtctgaaat cagtgttgca
     2221 ttgaatgacc ttatgttttc cattgagcaa tctacataat gaatgattat gtcaacgttg
     2281 gaatcatttt gccccccgaa tctttaatat gaacagtgca aagacaacgc tgagataata
     2341 gagttcatgg aagagctgga ttcgatgtca taacctcgcc gggcatgctc aataccgcct
     2401 gcaggttcgt gacgatcccg aagttcgccg cgatgaaaga aacccatgga aaaagattat
     2461 ggaagctttc accggaaggg gtaatgtgat catccagtaa atcgttccga caatatctcc
     2521 gtctgcttat aaaatttcta atgtaatttt ttctggtata tgataatatg ataaataatc
     2581 caataattct gcaaaaattg acctggcatt tacagccggc aataggattc tgtctttgga
     2641 cgctgtaata gatcgggcaa cataaacagc tgggggaata gccatgtgta atctacctga
     2701 tcttgtcgta aatattgcta ttggtggctg taattgcggg aatgtccccg aggatatcct
     2761 gaaaaggcag gttgacaggg tcctgtatga tacgatagca tgcgcgcagg aattcaacaa
     2821 tccggattat cgtttttccg gtgccgatat aaaagtgaac ctgttttcaa acccgttcat
     2881 ggcagatgct ttctggatcg attttgccga cagggggaac atgccgctca atatcattgt
     2941 tcccaatgac gagaagtccg gcgagttccg gacgctgnnn nnnnnnnacg ctgatcgata
     3001 aagccggttc agtgacaaat atcggcgcag acagtttacg ctcggccgga agcggttcgg
     3061 taatggccga ctggatagcg gaccaggcgg atctttccat ttttctgtgg gatggaaggg
     3121 aagaattcaa cagccgtgaa atatggacgc tgatccaggc gtgtaaaaga aacaatatac
     3181 cttccgtctg gatagatatc aattcacctg aaagtatcta ctggacactg gattcctatt
     3241 atgatgaata tacgaaggcc aggctccggg aatatattgc gaaattattc tgtgaggacg
     3301 gtgatgcctt cgatcctccg aatgaagcta aaattccgct atggcagctg tgggaccgcc
     3361 tctatggcag ctttatgaag aagtataggg ctaaagtcga acccgtccca tatatctgtg
     3421 acaaaatcat ggctaaggag ccgatcgaaa atgccgtcga tgaaaaatga gaactaaagc
     3481 gccggcagat agtcggctgg tttcattatt tcgacgacaa tgcggtcacc tattcacaga
     3541 aataccgtac ttcgatctac ttaagatcta taatgccgtt tattgctacc gtgtttatat
     3601 cggtcggctt ttatgcggaa agcatactgg ggtttattta tcagatcccg tacagctata
     3661 tcagcccatt ggctgttatc gcgggaatcg gtttcctgat ccacgcccta ataaactatt
     3721 acatgtacga tttgtccgaa aatccaaatg tgacaagctg gcatatccgg tttatcgaca
     3781 accgtttcat tgcggaggtt ttgaggctga cagcgcattt tgcacccttc gggatacccg
     3841 tgaaccacat ttcatcgttg aacaggttcg ggaacaaggt ctctcaaagg ccccatattt
     3901 caggcgaact gcgcaggatc acaaagagca tggaacctgc ggactttgtg ttcgaccggc
     3961 atacgtccgc agatttgctg gaaaatctca ctaatatgat agacgaccag atcgcttacc
     4021 atgaccggac ctaaaaaggt atgccaccat cgtttccagg ctgaaaaaat actcggcggc
     4081 tatttttgcg gtcgggttcg tcatagttgt tttgcgcgga ggcctgcagt tcgcgctggt
     4141 ttatgcaaaa actgacattg cccgcaatgg tatcaacctg atcagctttg taaaatcctt
     4201 tgccaacatg ctggccctga tggtcccgtc atgggcatcc tatttctctc ttaagctttc
     4261 cctgggcaat tttgaaggcc tggcaagcaa cagcagggcc atggctgacg gattggccat
     4321 gatgaagaaa atgctggacg atgaaaaaaa acggacagat atcagctttg aaaggatcta
     4381 cgctttttcc aaagacattt ccaggctcat gctaggagaa gtggttgaat ggtattcgca
     4441 gctgtcttcg tataaactga ccaaactctg attcaaaaga aaggaacgaa gcatatgggg
     4501 agaaagtatg acgtatttat ttcgtacagg cgccagggag gcgaacaaac tgcaaggata
     4561 atctgcgacc ggttgatgga ttcggggtac aaagtattct ttgatgtgga atccctgcgg
     4621 ccgggtgcat tcaacacgaa actttattct gttatagacg agtgccgtga tgtgatcgtc
     4681 gtactttcac agaacagcct tgaccgctgt aaagacgaga acgactgggt gaggcttgag
     4741 atcgcccatg ccctgaaagc ggggaagaac gttatcccgg tgttcctgcg cggttttgtg
     4801 ttccctggaa cactgcccgg ggatatagag cctttgaggt atcagaatgg cctggaggcc
     4861 agttcggagt tttttgacgc gtttattggc aaattgaaaa cgtttttgaa atcaaagccg
     4921 actttgctgc ggcgggtaag tcagaacgtc gttttcaaac gcactcttcc gttcctgctg
     4981 gcattgctta tcgtgctggg aacagggacc ggggcttacg tggtgatccg aaacagggca
     5041 cagacatttc ccgggacacg ggagcagaag aatatcacca atgaagcctt aagttatatc
     5101 gagaagaacc ttgtgctgat agacggggct tttggcgagg tttcagcggc atataaagcc
     5161 tgcggtaact atcttgccag tatggatcag gcacattatg aagaagctgt ggccactatc
     5221 aacagggcat atgggaacat aaaccggctg aatttttcgc agtatgccct gtcaggcact
     5281 gcattggaaa agatcgacaa cactccgatc aataaggctg acctggtaga ggtgaacgcg
     5341 cagaaccaga catagcgccg aaggatgatg acgaaccggg gatactgtgg ggtaagatgc
     5401 tgcgctttat aagcctgcaa ctgtatgatg aagcggaaaa atgcgcccag atttaccagc
     5461 agaaggtgca gggcgattat cccgaggcga aggaatacgt cccggccgct atccggttta
     5521 tcaggcagat ctcctcaacg ggcgtggact atggagtgat cgtctgcggt tatgagcctg
     5581 gaaagcccag gcatgaagtt tatgagatag gggatattat tgtcgcgata aacggtgtaa
     5641 tgtgccttaa tcttaataag tactcagaat tggtccccaa gggccaggaa tacagcgcga
     5701 aggttctaag gccggatgcg gaaggaaagc tggaattcct ggagttgact atcccaccag
     5761 gacaatccag gatcctgttg atgaacatga ctgaaagcga ataggggcgg cagagccaag
     5821 aactgagcga tatggcagat aaccgggcgg taaaggagaa acagaacgat gatcgaaaag
     5881 tatatcgatt cctttttcga tgaactggaa gtatttgatg atctggcaaa aggccgctcc
     5941 aacaaggcgt accttaaggg ttccatcatg gaattcctgg gggatggaaa cagggaaaat
     6001 gccatgaatg tctatgtatc cttttttgac agctaccgga tagtcctgga aggtgaaagc
     6061 aaccagttca tcgatctcct ggacgtgctc aggaattatg aagaaaggcg tcgaccctgc
     6121 ttgacaggca cagggaccac tatgtgcatt ccgtgaacgt tttcattctc ggactctgca
     6181 tatatatcag gaacggctcg ttccggaagg cattcgataa ggtcggcaga aataaagaga
     6241 tatataaata ttcctatgat accaggcacg aagagttcct gtaccgctgg gggatagcgt
     6301 cgctcttcca tgatgtgggg tatccggtcg aaatcacagc gaagcagatc ggcaaattcg
     6361 tcagctttat tacggacact gtagatgggg agagaagagt aaaagccgga atcgatctgg
     6421 ataccttcga agatttcaac agcatacctg aagtgatacc gaaagaggaa tttacgaaag
     6481 agttttcccg gaacgtgtct gaaagctccc gcatcgatct tctgaagccg ttggatttga
     6541 ttgcccttaa aatacataag gatttccatg tcgatctgga aacggccagg aaatcgctgg
     6601 acagatattt tgatgccatg aagaagtccg gcattgccga ccatggtttt ttcagcgcga
     6661 tcatagtgct caaatggtat ggcttcctga tccagaagtg cgggtacaac ccgaattatt
     6721 tcttctatcc ggtcgttgac tgtgcaagtg ccatcctgct gcacaattat tacaggaacg
     6781 tattgatgaa agaaccgttc cggctcggaa agctttctcc tgaactgaac cctgtggcat
     6841 atcttctgat cctgtgcgac gaactccagg aatggaacag gagggcttac gggaccatcg
     6901 acaggaagcg ggcatcggcc ggcaatataa aagcggcgat ttcgaacagg gaaatcagcc
     6961 tgacgtacat ttctcaaaac ggaatgctcc ccgacagctt tgcagcggaa aaaacggaat
     7021 tgcttgaccg tgtgctggat atcggcagta tcttcccggg cgggatccat ctgtcatgcg
     7081 agggaaatgg agatacaccg gcaggattgc aggatgaagc agccgcagac gataccggaa
     7141 cggatatgat cgttccgagg ccgttgctgg aaaacctgga gaagctggca agaatgatcc
     7201 atcaggagta caatgcccgg caacttgaga ggtatccgga aagaaaactc gaatatccaa
     7261 cgtgggagag ccttcctgat gacctgaagt actccaacat gaggcaggcg agaaggatcc
     7321 tcgataagct gaggctgatc ggatgcacgg tcatgccgaa gggaagcgga agcagccggg
     7381 taagccgctt cactgacggc gagatagaat tactggcgga agctgagcac gatttctggg
     7441 tcagggaacg cctgagccag ggttggaagt atggagcaaa gaaggatgtt gataaaaaga
     7501 tctcgccata cctggttcca tacgaacagt tggatgagga agcaaaacag cttgaccgtg
     7561 atacggtgac gaatatcata ccccttcttg agtcaatcga tcttgaggtt tgcagaatct
     7621 cgaacggaag aatgattgca aaagaaaaag gcatataaag caaacgggac tggagaccag
     7681 gcacataaga agagctgccg tgtaatctgt tcggagcccc aatgcaacct accctgataa
     7741 tcaaaaagga gcaggtaaaa cctgctccct tttgaatctt cagcacgtta tccgtttatt
     7801 tcacatcgtg cgtattatca gcataaagta ctgttactct tcttccgctt cttttgcctg
     7861 cttcttctca cctatgctga ggatcaggtt cagcaggatg ccgaatatcg cggcgcctgc
     7921 tatgcagggg acctctatgc cgaagaacgg tatgttgcct ccgaaggcgt agttcccgcc
     7981 aaggcctatg accatgattg ttgcgatcac ggcaatgttc ttggggctga acatgtcgac
     8041 cttcttctcg atcatgatgg cagcgccctg tgccgctatc gcgccgaaca ggtagacctc
     8101 gagtccgcct attacagcca ggggtatcga atatataccc tggaccagcg gtgtgaagaa
     8161 cgagatcacc attgcgatga ccgccgccag tgcaaggact gatacggaga acactttggt
     8221 gattgccatc gtgcttatat tctcaccgta gttggttcct gcagggcctc ctaccaaacc
     8281 ggagatcata tcgccgatac cgtcgcctat cagattgatg tcgagttttt cagcgaggtt
     8341 gtattttttg ctggagcctt ttttctttgc gaggtcgttc acatatacat caagctggta
     8401 catgtgtgca gtggattccg gtatcgtagc gatagcgatc ggcatgatcg cgaccattgc
     8461 cgccaatgaa ggtttgggca gtgtgaatgc gggcaccgct atcggacctg catgccagag
     8521 cgaagcctca agagcttctt ttggtatttc cctgaagaga ttgaaatcag cgccgccgac
     8581 agcatatata atgcctgcgg ttatgcaccc tactgctgct cccagaagca gcggcagctg
     8641 tccgaggaag cctttgagat atcttgagaa gagtatggtc gataacagtg tgaccagtga
     8701 aacgacccat acccagttgc tgttcatgac tgtcgcagca gccccttcga cgaccggttt
     8761 cggtgctgca tcgctcaggg cggtgccggc gagagtgagt ccgattatca ttgcgacagg
     8821 gcctgttatg ctcggaggaa gtattttttc cactgccgca ctgccgaagg ctctaaccag
     8881 aaagcctgca gcgatggata taagaccgga cattatgata ccaaactgtg cataagatat
     8941 ggcttctgcc ggaaggatgc ttgttgtgcc ctgtttccat gcttccagct tttccagcgt
     9001 tcccgggctg accatcgtct ttatcgcttc gcttgtcatc aggccggtga tcgctgtaag
     9061 gtatgagaaa ctggagccat agtaaagagg tatctgtctt cctgtaatga gaatgaaaca
     9121 taatgtggca agaccgctgg cgaagattgt cgttgatacc tgaaaaccgg tgatgagtgc
     9181 cacggctacc gtagcaggga acatgactat tacctgctgg attgcgtaaa acaacagttt
     9241 ccagaatggg ggcctttcat cgggcagata cccgaccact gtgcgtgctt tctgcatggg
     9301 gattcctcct tgttataaaa tttgtcagag cattaaaaaa tcccgccggc agaacgcagg
     9361 caagaccgct tgaatcgcat catccctgat gaacatcaga tgggcatacg gaatcaatat
     9421 agcgtcttac cggcatctct gtaccggact taaagacttt ttgttcgtcg acattatatc
     9481 attcacataa tcgcttttca ataaaaattt tcagattttt tcacaacttt aaattgcctt
     9541 catttgaaaa agtaatttcc acttcaaaat caatgacagt ggaattaact t
//
LOCUS       DAOSCC010000084         9580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4677_length_9580_cov_4.716850, whole genome shotgun sequence.
ACCESSION   DAOSCC010000084 DAOSCC010000000
VERSION     DAOSCC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_9580_cov_4.716850"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..813)
                     /gene="spoIIM"
                     /locus_tag="PLG72_05975"
     CDS             complement(172..813)
                     /gene="spoIIM"
                     /locus_tag="PLG72_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOJ80380.1"
                     /translation="MVKKLHNIIREQMNNNRNTYFFLLISFIFGVITGAFTVNGLSAM
                     QRDELSNYLHGFLQLFGNQNVNSGELFSAGLIENLKMIGLLWVLGVTVIGIPFIYLIL
                     CVRGFVTGFTSGFVINALGMKGMLFTGFALFPKEMIIIPCLIGIGVNGINFSMRLVKN
                     RTVTEHQKPGLKSAFLSYCLVSLLFTCLAMVGIMVDAYVTPVLIRMIAPMVTM"
     gene            complement(1059..1616)
                     /locus_tag="PLG72_05980"
     CDS             complement(1059..1616)
                     /locus_tag="PLG72_05980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ80381.1"
                     /translation="MNYYEKTVSEKHIYKGNIIDVDLLTVMLPNGKTATRDIVRHPGA
                     AAVIPLNEKGEVYMVRQFRKPLDSVSLEIPAGKLDKGEDPIVCAGRELKEETGLTAEK
                     LTHLVSVHSTPGFSDEVLHLFAATGLKEGDSCTDEDEFITTEKYHVDELLGMVLEGKI
                     TDAKSIIGIFLADKIVSGKINIPYK"
     gene            complement(1613..2092)
                     /gene="rnhA"
                     /locus_tag="PLG72_05985"
     CDS             complement(1613..2092)
                     /gene="rnhA"
                     /locus_tag="PLG72_05985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOJ80382.1"
                     /translation="MADKRKKVRIYTDGACSGNPGKGGWAAILLYEGHEKVLSGFEPD
                     TTNNRMELTAAIEALRALKYPCSVELHSDSAYLVDAFNNGWITGWKLNGWINSSKDEV
                     KNRDLWQKLDELAGIHDVTWIKVKGHSDNEYNNRCDKLAVNEIKKNSADSTEGEDDK"
     gene            complement(2099..2920)
                     /gene="proC"
                     /locus_tag="PLG72_05990"
     CDS             complement(2099..2920)
                     /gene="proC"
                     /locus_tag="PLG72_05990"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ80383.1"
                     /translation="MQTKIGFIGTGNMGASILRGILRSGTVRHENIYIFDTDKSKMEA
                     LAKETGVTACSGNAELVRICDYIILAVKPAAVPQVLAEAKDSFTDEKVLVSIAAGISI
                     DNLRRHAGKDIKIVRTMPNLPLMVGEGMTVISFGDHISEEEKRTVKELFRSSGEAEEL
                     DEKLMNEVIALTGSSPAYVFMMIEAMADGAVQQGIPRQAAYKLAAQAVLGSAKMVLET
                     GLHPAELKDRVCSPAGTTIEAVNELEKCGFRHAIMSAMKACTDRAAEIGRSTESR"
     gene            3143..3218
                     /locus_tag="PLG72_05995"
     tRNA            3143..3218
                     /locus_tag="PLG72_05995"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3176..3178,aa:Trp,seq:cca)
     gene            3383..3859
                     /gene="tnpA"
                     /locus_tag="PLG72_06000"
     CDS             3383..3859
                     /gene="tnpA"
                     /locus_tag="PLG72_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOJ80384.1"
                     /translation="MKDTSSLAHTKWNCKYHIVFAPKYRRQVIYGKIKSDIGKILRKL
                     CEWKGVNILEAEACPDHIHMLVEIPPKISVSSFMGYLKGKSSLMIFDQHANLKYRYGN
                     REFWCRGYYVDTVGKNKKAIQEYIKNQLQEDLAYEQMSIKELVDPFTGEPVKKSKK"
     gene            complement(4391..5242)
                     /locus_tag="PLG72_06005"
     CDS             complement(4391..5242)
                     /locus_tag="PLG72_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80385.1"
                     /translation="MKESKLSKILTLIVFSSLVISVIYIVIRIIIVPGGKAAAGERPE
                     SEYILMLLQCTAGIIIMFLPGFISKKFSLTIPSVMHIIFVIFLFCAIYLGEVRAFFYH
                     FKHWDVMLHGLSGIMLGALGFSFVTLLNDAERIPVNLSPIFVSLFAFCFAIMLGVMWE
                     IYEYVFDGLLGLNMQKFALEDGTLLIGRAALVNTMKDLIVDSIGALVSSALGYVSLKH
                     KTGWLDSVLIRKNKSKAEVQADAVMMDTEPNTDEEMHETVHVGNTASEAVPPAGSQFS
                     DKIGIGQ"
     gene            complement(5334..6314)
                     /locus_tag="PLG72_06010"
     CDS             complement(5334..6314)
                     /locus_tag="PLG72_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80386.1"
                     /translation="MKRDSTDGGTLSGSGTENSPGDSGAYVPASGDTSDTDARGAAGE
                     EDDSGSVTDSDGPAEDSDMQNPRQSGNKPDYKLLQSASIDLDNDGVNEQIGVVRTDLP
                     MNEAGTESRAEGRLLISDGNNERQVVFFTKENDPSELLSRIEFEDLDGDGSTDIFMVI
                     PGYGASFNYSSYFIYSYKKDKSHSFISDNTLADFIDGFEFSYAKGASLLTVANRFHNF
                     SVDIVIEEAEEQESPEDIMQQYAQGTWIDPVSVDISEDSRLSLVKNKGLPEIKVPLPV
                     FGMATVNMIAEIDLFYGIDSDFIPVLKRCEIIDFSGSDKIKTGSFEVTEG"
     gene            complement(6704..7981)
                     /locus_tag="PLG72_06015"
     CDS             complement(6704..7981)
                     /locus_tag="PLG72_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ80387.1"
                     /translation="MKWTGRFIRFGCALLIVIMAVILSGCHDMREIDDMAYPLAIGLD
                     IGHANVLRMTLQLAAPLSIGGGGAGESGGGGETGGGGKPISVITIDTPSIFSGLNMVN
                     NIISKEINMSHAKVVVISRDLAEKGVEKYVQAMTRGREFRPDMFIVVSNDPPDEYLKE
                     VSAILENNPAKYYELLLGKEFSSYYPEARMSDFHNAFERDDVEAVAVLSGINPKESVE
                     QIDEMSRIGRKYLDEGRYEAGDVPMISDLRIVAMGTAVFKSGKMVGTLNGIESVCLEM
                     VTGKYRHSYWTIPDVYDKNNVIVMNVVQRKKPKATVKLQDGKPVIDIVLDLEGDFTSL
                     QSTAEYEKYPELVENKTSEVIRQNIMSFLRRTTDEFDSDICGFGRYIKGRFLTWDEWK
                     KFDWPSHYKKSEFNVTVNFKIRRTGLIIRQMMS"
     gene            complement(7978..9081)
                     /locus_tag="PLG72_06020"
     CDS             complement(7978..9081)
                     /locus_tag="PLG72_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOJ80388.1"
                     /translation="MEKKIKFGRWEAVALTSNLICTKVFLYFNRLTVEDAGNAGWLMA
                     IFICAVAVALYSLQIWLYKKFEGKDILDIAEIAGGKILKAITGFTLGGLLFLLAAITI
                     RKFSEDIKTVSLPTSPLSYVMVFFMAGIVIAGFLGVESIVRHSAIVIPVVVAGYIVIL
                     LGIMPKIDVTNLFPIFGTGVKNVLLKGFYRTSIFAELLVLFMLPPFLGGYEDVKNIGY
                     VSVGLCSFFLITGSLVYILVFPYPSSLEPFLPVFNMARLIGLGRFFQRIESLFVFIWV
                     MSALMYLSAVFFFATYTVSKAAGLKYIRPMIIPFAIIVFSIAFLPPDLVSVIKIESGF
                     VNTWGWVVTFVFTGLIFLWARIAKRGRRENRGK"
     gene            complement(9118..>9580)
                     /locus_tag="PLG72_06025"
     CDS             complement(9118..>9580)
                     /locus_tag="PLG72_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ80389.1"
                     /translation="EAGLRVPGPIGPTLGIIGALILGQAAVAANIVSPILIIIVAVTG
                     IGSFAIPNYSLAYSVRIMRFGFILLGALAGFLGITLGLFIIGTWSTALKSFGVPFMAP
                     LGPRTSGRYKNVIFKAPEWKLEDRPDYLNTKKKRKQDEYSMKWKRDTEKKK"
BASE COUNT         2473 a         2450 c         1956 g         2701 t
ORIGIN      
        1 attttgcaat aaatatttgt ttatgtaaat attatatacc gttttctcta tattatactt
       61 tcattttctt attttttcat ttttttcttg cctgagccgt attccttttc ctgggtattt
      121 ccaggcaatc ctgtctgacg tcaaagtagc ccatgcacaa tgccgctgta atcacatagt
      181 caccatcgga gctatcatgc ggatcagcac tggtgttaca tatgcgtcaa ccattatccc
      241 gaccatagct aaacaggtaa acagtaaaga cacaaggcaa tatgacaaaa atgctgattt
      301 cagtcctggt ttctggtgtt ccgtaaccgt cctgttcttt accagccgca tcgaaaaatt
      361 gatcccgttg actcctatgc cgatcaggca tggtattatg atcatttcct tagggaataa
      421 agcaaaacct gtaaacagca tccctttcat ccccagcgcg tttattacga atcccgaggt
      481 gaatccggtc acaaagcccc ggacacataa aattaaatat atgaaaggaa tgcctatcac
      541 agtaacgcca aggacccaaa gcagtcctat cattttcagg ttttcgatca aaccggcaga
      601 aaacagttcc ccgctgttga cgttctgatt gccgaacaac tgcagaaagc cgtgaagata
      661 attggacagt tcatctctct gcattgcact taatccattt acagtaaaag ctcctgtgat
      721 cactccgaaa atgaacgaaa tgagcaggaa gaaataagta ttgcggttgt tattcatctg
      781 ttccctgata atattatgaa gctttttgac cataaccgtt cctcccgaca tgaatccggc
      841 gtctcccgga cgcagcatga ctgactgcat catttttcca atggtttttc atgaacggta
      901 ttgacgcttc aatacgatcc ttttgtctta agaaaaaaat ttacaatatc atcatatgcc
      961 gatgatattg taaactattc ctgccgaaga tttgtcataa gagtgttggc attggatgtt
     1021 gcttataagg gaaagagatg acagttcaca ttggatattt atttatatgg gatattgatt
     1081 ttgccgctta ctatcttgtc tgcaaggaaa atgccgataa tgctcttcgc atctgtgatc
     1141 ttcccttcga gcaccatgcc gagcagttca tcaacgtggt atttctcagt cgtaatgaat
     1201 tcatcctcgt ccgtgcagct gtccccttcc ttcaggccgg ttgcagcgaa caggtgcagg
     1261 acttcgtccg aaaatccggg cgtgctgtgg acacttacca ggtgtgtcag tttttctgcc
     1321 gtaagaccgg tctcctcttt cagttctctt cctgcgcata cgatcgggtc ctctcccttg
     1381 tccagcttgc ctgccggtat ttccagcgag acggaatcga gcggttttct gaactgcctc
     1441 accatatata cctcgccttt ttcattcagc ggtatcacgg ctgccgcccc gggatgcctc
     1501 actatatcgc gggtcgccgt tttcccgttc ggaagcatta ccgtaagcag gtccacatct
     1561 attatattgc ctttatatat atgtttttcg gaaaccgtct tttcataata attcatttat
     1621 cgtcctctcc ttccgtcgaa tccgccgagt ttttctttat ctcattgact gccagtttgt
     1681 cgcacctgtt attatactca ttgtcgctgt ggcctttgac ttttatccat gtcacatcat
     1741 ggatacccgc cagttcgtcc agcttctgcc aaaggtccct gttctttact tcatccttgc
     1801 tgctgtttat ccatccgtta agtttccatc cggtaatcca gccgttgttg aaagcatcca
     1861 caagataggc actgtccgaa tgcagttcga ccgaacacgg gtatttcaga gctctcagag
     1921 cttctattgc agcggtcagt tccatcctgt tgttggtagt gtcaggttcg aaaccggaca
     1981 gcaccttttc atgcccctca tacaacagta tggctgccca gccgcccttt ccggggttgc
     2041 ctgagcatgc accgtccgta tatattctga cttttttcct tttatcggcc atacctgttt
     2101 acctgctttc agtgctgcgg cctatttccg ccgccctgtc agtgcaggcc ttcatcgcgc
     2161 tcattatcgc gtgcctgaat ccgcactttt caagttcatt tacagcctct atcgtagtgc
     2221 ctgcaggaga gcagacccgg tctttcagtt cagcaggatg caggccggtc tccagcacca
     2281 ttttcgccga tcccagcacc gcctgggccg ccagcttata tgccgcttgc ctcggtatgc
     2341 cctgctgcac tgccccgtca gccatggctt ctatcatcat gaaaacataa gcagggctgc
     2401 tgccggtaag cgcgattact tcgttcatca gtttctcgtc cagttcctcc gcttctccgg
     2461 aacttctgaa gagttcctta acagtccttt tttcctcttc gcttatatga tcgccaaagc
     2521 tgatcacggt cataccctcg ccaaccatca gcggaaggtt cggcattgtc ctgacgatct
     2581 ttatatcctt gcctgcatga cgtctcagat tgtcgatgct gattccggca gctatggaga
     2641 caagcacttt ctcatccgta aaactgtcct ttgcctccgc aaggacctgc ggaactgctg
     2701 caggtttgac tgcaagtatg atataatcac atatcctgac aagctcagca ttaccggagc
     2761 aggcagtcac acctgtctcc tttgccaatg cttccatttt gcttttatca gtatcgaata
     2821 tgtatatgtt ctcatgtctg accgtacccg aacgcagaat gcctctgagt atcgacgcgc
     2881 ccatgtttcc ggtgcctatg aacccgattt tggtttgcat acacatctct ccaatccgta
     2941 tgattctgat cgaacagcat gtccatcctt tgaccgcgtc aaggctgatc catatctcat
     3001 cccgtatata atagcataca gtttgaagga aattccacag ttattttaat tcacaccatt
     3061 gacacaagtc cgggatatat tgtagaatat aaaagtccac cgggagagcc ggcggattga
     3121 tttattgatg ataaatgaac ataggggagt agctcaattg gtagagtggc ggtctccaaa
     3181 accgtgggct gcgggttcaa gtcctgtctc ccctgccaga atgccaaggc ttttgagcct
     3241 tggcattttc attgtgtaac aagtaaacca cttgctaagc aagtggaaga aaaaaggctt
     3301 atgccgttga gcataaaaaa ctcctatgtt agtataagtg caggtctagc caaccgcaaa
     3361 aatacaacat aggaggcgtc ttatgaaaga cacaagtagt ttagcccata cgaaatggaa
     3421 ttgcaagtac cacatagtat ttgcaccgaa atatcgtcga caggttatat acggaaagat
     3481 aaaaagtgat ataggaaaaa tactgaggaa gctatgtgaa tggaagggag taaacatact
     3541 tgaagctgaa gcatgcccgg atcacataca tatgctagta gaaataccac cgaaaataag
     3601 tgtatcgagt ttcatggggt acttaaaggg aaaaagtagc ctaatgatat ttgatcagca
     3661 tgcgaacttg aaatacaggt acgggaaccg agaattttgg tgtagaggat attatgtgga
     3721 tacggtgggg aagaacaaaa aggcaataca ggaatatata aaaaatcaat tacaagaaga
     3781 cttggcatat gagcagatga gcataaaaga attagtggac ccgtttacgg gtgagccagt
     3841 aaaaaagagc aaaaaatgaa gccgctttag cggcagcttg gaaaccaatg cagttggcgg
     3901 actattcgta gggctttcag ccctgccagt aataggccct tacagggcaa gagcaaaccg
     3961 ccggctaagc cggcggtttt gattgactat tcttatgttg acgttttgtt gacacacatt
     4021 ttagtaatag tttggtattt gcatcttttt ccttcagatc gatcctccga ttgttttatg
     4081 tggttatgtt cacttccttt attatgtcga tgccggcttt ccttgctgac aactcaaagg
     4141 cttgtcttgt tacagcaaaa gaaaggattc tctacgtttt cgttctcgag gagtcattca
     4201 gttgtctttt aatagtaatg tttgtaattt tattacaggg agatacatct gaacgtaacc
     4261 gcaggataaa cagtagatat atcaaacagc cgactcagta taaagcccca aaatggggct
     4321 tttttcatga gattctgttg aattgaacat ttcggatcag caacgattct gatcctctgt
     4381 gtatcgaacc ctattgtcct atccctatct tgtccgaaaa ttggcttccg gcgggaggaa
     4441 cagcctctga cgcggtattg ccgacatgca cagtttcatg catttcttcg tcagtattcg
     4501 gttcagtatc catcattacc gcatcagcct gcacttcagc ctttgacttg ttcttcctta
     4561 taagcaccga atcaagccag cccgtcttat gcttcaggga aacatagccg agggctgatg
     4621 agaccaaggc tccgatggaa tcgacgatca ggtccttcat cgtattcacg agggctgccc
     4681 gcccgatcag caatgtgccg tcctccaggg cgaatttctg catattgagg cccagcaaac
     4741 cgtcgaaaac gtattcatag atctcccaca tcacaccaag catgatagcg aagcaaaacg
     4801 cgaatagcga gacgaatatc gggctcagat tgaccgggat ccgctccgca tcattcagca
     4861 acgtcacaaa tgagaaaccc agcgcaccaa gcatgatgcc gctgagtccg tggagcatca
     4921 catcccagtg cttgaaatga tagaaaaagg cccgcacttc gccgaggtat atcgcacaga
     4981 acaggaaaat aacgaaaatt atatgcatca cgcttggtat cgtaaggctg aatttctttg
     5041 atataaagcc cggcaggaac attatgatta tacctgcggt acattgcagc aacatgagta
     5101 tatactcgct ttcaggcctt tcgcctgctg cagctttacc acccgggact attattatcc
     5161 ttattacaat gtatatgaca gatatcacaa gagaactgaa tacaatgaga gtcaatatct
     5221 ttgacaattt ggattccttc attattgcct cccctttacc gatcagcctg ctctatatat
     5281 ctggatccat cctgtaaaat gtgctccgcc tctgacctga tgcggcaacg cctctaacct
     5341 tccgttactt caaaactgcc tgtttttatt ttatcactgc ctgaaaagtc gattatttcg
     5401 caccgcttaa gaaccgggat gaaatcactg tcgatcccgt aaaaaagatc tatttctgca
     5461 atcatattca ctgttgccat cccaaatacc ggaagaggca cttttatttc cggtaatccc
     5521 ttatttttaa ccagcgacag cctggagtcc tcgcttatat cgactgagac aggatcgatc
     5581 cacgtccctt gcgcatattg ctgcatgata tcctcgggcg attcctgctc ttccgcttct
     5641 tcgatgacaa tatcaacgga aaagttgtgg aacctgttgg caacagtcag caaactggcg
     5701 cctttggcat aagaaaattc aaaaccgtcg ataaaatccg ccagcgtatt gtcactgata
     5761 aatgaatggc ttttatcctt tttgtagctg tatatgaagt aactcgaata gttgaaggac
     5821 gctccgtacc cgggtatcac catgaaaata tccgtggacc cgtcaccgtc aaggtcttca
     5881 aattctatcc tgctcagcag ttcagacgga tcgttctctt tcgtgaagaa aacaacctgt
     5941 ctttcattat ttccgtcgct tatcaaaagc ctaccttccg ctctgctttc cgtaccggcc
     6001 tcgttcatcg gcagatctgt cctgactacc ccaatctgtt cattgacacc gtcattgtca
     6061 agatcgatac ttgcgctttg cagcagttta taatcaggct tattgccgct ttgccgcgga
     6121 ttttgcatgt cactgtcttc tgccggtccg tcgctgtctg taacgctgcc cgaatcatcc
     6181 tcttctcctg ccgctccccg ggcatctgta tctgatgtat cgccggatgc cggcacataa
     6241 gcgccggagt cacccgggct gttttcagtt ccgcttcccg aaagtgtacc gccgtctgtg
     6301 ctgtcccgct tcacaatgcc gcatccggca gccgccagaa gcagaattga caatatcaat
     6361 gatattaaga ctacacgcct gatattatcc ccccctgtct gaacacaaag cgaaacacgc
     6421 cagtatatcc ggcctggtcc tggttattat aataccatca tttgcggcat taagccagct
     6481 atgttctgaa aaataatata tatgcaggaa gtgcgatcat ggccgtgcaa acaagaatta
     6541 caaatattga gccggcttta ttgttatttt tccagttcca taccgcatag ctgacagtat
     6601 gtatcagcgc atatgcaagt accaatatca atgcgatcca tgtcaaaagc gacataacgg
     6661 tcctcctgaa cgaaaatcac ggcgttgcat aaatgtctcc ccatcacgac atcatctgcc
     6721 tgattatgag acctgtgcgt ctaatcttga aattcactgt cacattgaat tcactttttt
     6781 tgtaatgcga aggccagtca aactttttcc attcatccca tgtgaggaac cgtcccttga
     6841 tatatctgcc gaaaccgcag atgtcggaat cgaattcatc tgtcgtcctc ctgagaaagg
     6901 acatgatgtt ttgccgtatg acttcactgg tcttgttttc caccagttcc ggatactttt
     6961 catattctgc ggtactttgc agagaggtaa aatcgccctc gagatcgagc actatatcaa
     7021 taaccggctt accgtcctgc agcttaactg ttgctttagg ttttttcctt tggactacat
     7081 tcatcacaat gacattattt ttatcataaa catcaggtat tgtccaataa gaatgcctgt
     7141 atttccctgt gaccatctct aaacatacgg attcgatccc attcagcgtt ccgaccattt
     7201 tgccggattt aaacacagca gtgcccatgg ctactatcct gagatcggaa atcatgggta
     7261 cgtcccctgc ttcatatctt ccctcatcca gatatttcct tccgatccgg ctcatctcgt
     7321 caatctgttc cacgctctcc tttggattta ttcctgacaa cacggcaaca gcttcgacat
     7381 cgtcacgctc aaaagcattg tgaaaatccg acatccgggc ttcaggataa tatgatgaaa
     7441 attctttgcc gagaagcagt tcataatatt ttgccggatt gttctccaaa atggcgctta
     7501 cttccttcaa atattcgtca ggagggtcgt tcgaaactac tataaacatg tccggcctga
     7561 attctctgcc cctcgtcata gcctggacat acttttccac tcctttttca gccagatccc
     7621 ttgatatgac aactactttt gcatgcgaca tattgatctc tttgctgatg atgttgttaa
     7681 ccatattgag tccggaaaaa atcgaaggcg tatctatggt gattaccgat atgggctttc
     7741 ctcccccgcc tgtctcgcct ccgccgccgc tttcaccggc ccctcctcct ccgatggaaa
     7801 gcggcgccgc caactggagc gtcatcctca gaacattggc atgccctatg tccaggccta
     7861 tggcaagcgg atatgccata tcgtctattt cgcgcatatc atggcagcct gacaggataa
     7921 ctgccatgat cacaatcaat aaagcgcaac caaaccgtat aaatctccct gtccatttca
     7981 tttgcccctg ttctccctcc ttccgcgttt cgcaatcctg gcccacaaaa agatcaggcc
     8041 agtaaaaacg aaggtcacga cccaccccca ggtattcacg aaacctgact caatttttat
     8101 taccgataca aggtcgggcg gcaggaatgc gatactgaag acgattatcg caaaaggtat
     8161 tatcatgggc cttatatatt ttagcccggc ggcttttgaa accgtgtacg ttgcaaagaa
     8221 aaaaactgcc gaaagataca tcagcgccga cattacccaa atgaaaacaa acagcgattc
     8281 gatcctttga aaaaacctcc caaggcctat aagccttgcc atgttgaata ccggaaggaa
     8341 tggttccagg cttgaaggat aagggaatac aagtatgtaa accagggatc ctgtgattag
     8401 gaagaaagaa cataaaccga cggaaacgta tccaatattt ttgacgtcct catagcctcc
     8461 caggaacggc ggaagcatga acagcaccag aagctcagcg aatatcgacg ttctgtagaa
     8521 tcctttcaat aaaacatttt tcactccggt accaaatatc gggaaaagat tagtaacatc
     8581 tatcttgggc atgatcccaa gcagtatcac aatgtagcct gcaaccacga caggaatcac
     8641 tatcgcacta tgcctgacga tcgattcgac tcccaggaaa ccggctatca ctattccagc
     8701 cataaagaag accataacgt aactcagcgg agatgttggc agcgatacgg tttttatatc
     8761 ttcggaaaat ttccggattg taatggctgc cagcaggaac aggagccctc ccagcgtgaa
     8821 cccggtgatc gctttgagta tctttcctcc tgcgatttca gcaatatcga gtatgtcttt
     8881 gccttcgaat tttttataca gccatatctg caaagaatac agcgccaccg ccacggcaca
     8941 tatgaagatg gccatcagcc agcctgcatt gcccgcgtct tccaccgtca gcctgttgaa
     9001 ataaagaaaa actttagtac agataagatt gctcgtcaga gctactgctt cccatcttcc
     9061 gaatttgatc tttttctcca ttcgcattaa aatccacctg tccctgtttc tcccgtttta
     9121 cttctttttc tccgtgtccc gtttccattt catgctgtac tcatcctgtt tccttttctt
     9181 ctttgtgttc aaatagtccg gcctgtcttc caatttccac tcaggcgctt taaaaattac
     9241 atttttgtat ctcccggatg tcctgggccc cagcggcgcc atgaacggaa cgccgaatga
     9301 cttcagagcg gtagaccagg tgcctattat gaagagtccc agcgttatcc ccagaaagcc
     9361 tgccagcgct ccgaggagaa taaacccgaa ccgcattatc ctgactgaat acgccaatga
     9421 atagttgggg atcgcaaagg aaccgatgcc cgttaccgcg actatgatga tcagtatcgg
     9481 gctcactatg ttggcggcaa cagccgcctg tccaagtata agcgcaccta tgatgccaag
     9541 ggtcggcccg attgggccgg gcactcttaa gccggcttcg
//
LOCUS       DAOSCC010000085         9535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4704_length_9535_cov_4.906646, whole genome shotgun sequence.
ACCESSION   DAOSCC010000085 DAOSCC010000000
VERSION     DAOSCC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_9535_cov_4.906646"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PLG72_06030"
     CDS             complement(<1..1715)
                     /locus_tag="PLG72_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ80390.1"
                     /translation="MKKVRLIALLLVAVMIFGVMTACGKQETKGSGDQPATTPNSDTG
                     GTEPSVPAEEKKEPITFTMFQADPGEAPPADNKIIKKIQEATGVTIEIEYLVGDMEQK
                     VGIMIASGDYPDLIGPGQARGQFLNAGAFIALDDYLPKYPNLWKHYEPYLDVLRSVSS
                     DGKIYIMDIWSREYWLNDGEDTAYQVGYNGPAFWTQKDVLAWDNYSYPKNLDTYFDLL
                     ERYYKAHPTIDGQPTLGFEVHSQDWRSFCLKNAPQHLIGGRNEGDVVVKPDLTVEIYQ
                     NKDYAKAYYKKLNEMYKKGLINPETFSRNYDDYLSVISTGRVLAMFDQQWNFGNAENV
                     LISEGKYERTYVPLGISLDGNEALPYNRYKSAGIVGGNGMGISVKCKDVERALEFLDF
                     MLREDVQKLCYWGIEGEDYYVDENGRYRRTEEQRANFNNPDWVKANAGINFANLFPKI
                     QGRYSDGNAAGAGNQPEERIEGMSEYDKEFLAKYGFSDRSEFLPLAEYAGYPEVWSIF
                     STMDEDNPVKAIFNDITDLQNRYLPGVIMADDFESAWNEYVKRYENVDYPMLEKEIEK
                     EIKAR"
     gene            complement(2191..2748)
                     /locus_tag="PLG72_06035"
     CDS             complement(2191..2748)
                     /locus_tag="PLG72_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ80391.1"
                     /translation="MKENLQPEGRVVQDRSETGFPVISVSEIIPAYVYKIGIEQAQFS
                     FSTAVGLFNAVVNVFVLTVHCCSAAESDHKKNAGRVLRIDCKQRQGSFAHEQRELNVD
                     RMKIDKYFIDKLLYTEPDKEITSDIISMAHRLGHSTVAEGVEDMIQFQYLKEHGCDKV
                     QGYLVSKPLSEEDAISFLKGAGDRL"
     gene            complement(2994..5033)
                     /locus_tag="PLG72_06040"
     CDS             complement(2994..5033)
                     /locus_tag="PLG72_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ80392.1"
                     /translation="MKFKPIVPKFPHILHGGDYNPDQWRDVKEIWSEDMRLARLAHIN
                     TLTVGIFSWAALEPEEGRYDFSWLDEVMDLLAQNGIAAVLATPSGARPAWLSQKYPEV
                     LRVNEDRRRILHGGRHNHCYTSPVYREKVRNINTKLAERYKDHPALAVWHISNEYGGE
                     CHCDLCQEAFRNWLKERYGSLEALNAAWWTAFWSHTYTDWSQIESPSSIGETSIHGLA
                     LDWKRFVTEQTLDFMKAEMEPLRRLTPNVPCTTNMMGLYDGLNYYRLSEALDIASWDN
                     YPIWRSDDGDADIAILTSMVHDMNRRLKDGRPFMLMESSPSATNWQPVGKLRRPGLHE
                     LQSIQAVAHGSDTVQYFQFRKSRGSSEKFHGAVVDHVGTEHTRVFREVARTGETLEKL
                     DAIIGTSKPSDVAIIYDVENRWALEMCQGFKYNKKYIETLKEHYAPFWKSGISVDIID
                     STKELSGYKLVIAPMLYMLRPGMAEKIEDFVKSGGTFVATYMTGYVDEHDLCFLGGFP
                     GPLRKVLGIWCEEIDSLFDEERRHVEWNGTRYEARDFCEIVHTEGAKVIGTYCEDFYE
                     SSPALTVNSFGEGEAYFVAARLDEQFQDDFFGMLACKLGIAGAIEGNLPYGCTAQMRT
                     DGENHYVFVMNFTPEQKSIDIGDGGKSMVSGEEIKGSITLPPWGFDIYIKRNKGE"
     gene            complement(5398..6210)
                     /locus_tag="PLG72_06045"
     CDS             complement(5398..6210)
                     /locus_tag="PLG72_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ80393.1"
                     /translation="MLHGKNIFENIFVKNLISHVNYCAPDWGETDCSYGYNKFYYFIE
                     GDATLIIEGDEYHPEPEELFLIPADTRHTYYHDPVRPVYKYWCHFDLRLNEGRKLVYS
                     KYGAKCRIPRETIVPVFEKLISSYNSADPRDTLSEKAALLEILRLFMDNVDYVCILPE
                     NTDDFIAAVDEYIVRNIGSAITLQGLAGIVHLHPNYFIQYFRKRYNVSPMEHVKILRL
                     EKAAQLLVQHPEQSVGEIAMEVGFRDYRYFGRIFRKRYGITPSTYKRLAGGK"
     gene            6518..7975
                     /locus_tag="PLG72_06050"
     CDS             6518..7975
                     /locus_tag="PLG72_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ80394.1"
                     /translation="MQPEIRKGPFEPTFESLRTFECPEWYRDARFGIWSHWGAQSVPM
                     YGDWYARNIYCEGSDQYRYHIRRYGHPSKFGYKDIVQLWKAENFDPDGLMELYAEAGA
                     KYFVAQAMHHDNFFNYASKIHPWNSANMGPHKDIVGLWKEAADKKGLPFGVSEHLGAT
                     FSWYRVNKGYDREGPFAGVPYDGNDPEYESLYLPNREHYDPDKGHWDLDPWYTPNRWW
                     HQRWFEIIKEVIDLYQPDLLYTDGGVPFGDGMYETGLNVIAHLYNTSAAKHGGKNMAV
                     YTQKDRRKEIYTVGVLDIERSQSEEIRQEPWQTDTCVGHWFYDVRAAYKKPGHVIEIL
                     VDVISKNGNLLLNIPQKPDGTLDDECLYILRELAKWNRVCGEGVYGTRPFQVPGEGPS
                     RVHIEGFREDPVAWTGSDYRFTTKDKTIYAFQMKWPGNNRAVIKSLGPSEKVRAVRLL
                     GYGQVPFEQPYGTLVVSLPDKKPTEYVNCLALEME"
     gene            8017..9075
                     /locus_tag="PLG72_06055"
     CDS             8017..9075
                     /locus_tag="PLG72_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ80395.1"
                     /translation="MDKKTFKIADDGIDPNIVPFKVLYTGAKMPGIGLGTFGSDRYSG
                     EEIAAAVKEAISLGYRHIDCAAVYGNEHLIGKSLRDILESGLVKRDELFITSKLWNDM
                     HGDGDVLIACAKTLKDLKLEYLDLYLVHWPFPNHHAPGVGADSRDPHAKPYIHEDYMK
                     VWRQMERLVDAGLVRHIGTSNMTVPKLELLLRDARVRPAVNQMELHPHFQQPELFRYC
                     LDRGIVPVGYCPVGSPKRPDRDRTPEDTVDIEDPVIVRIAESHNVHPAIICIKWAVQR
                     GQVPIPFSTDRNHLRSNLLCAVTDPLTDEEMRKIEKTDRNCRLIKGHVFLWEGAKDWT
                     ALWDIDGVINGATEELDR"
     gene            9140..>9535
                     /locus_tag="PLG72_06060"
     CDS             9140..>9535
                     /locus_tag="PLG72_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ80396.1"
                     /translation="MGNLMKAAYLPGNSTVVLKEVEIPEPGYGQVLIRTKASTICGSD
                     IRAIYHEHLGKGPEGYQNKIAGHEPAGQIVKCGPGMRRFKEGDRVIVYHISGCGLCVD
                     CRMGYMISCTSPLRAAYGWQRDGGMAPYIL"
BASE COUNT         2383 a         2426 c         2254 g         2472 t
ORIGIN      
        1 taacgtgcct tgatctcttt ctcgatctct ttctcaagca tcggatagtc gacgttctcg
       61 tacctcttca cgtactcgtt ccatgcggac tcgaagtcgt cggccatgat cacgccaggc
      121 agataacggt tctggagatc cgtgatatcg ttgaagatag cctttacagg gttgtcttcg
      181 tccatggtgg agaagatcga ccatacttca ggatatcctg catactctgc cagaggcagg
      241 aactcggatc tgtcactgaa tccgtacttg gcaaggaatt ccttgtcgta ttcggacatg
      301 ccttctattc tctcttcagg ctggttgcct gcaccagcgg cgttaccgtc tgagtaacgg
      361 ccctggatct tcgggaacag gttagcaaag ttgattccag cgttagcctt tacccagtca
      421 ggattgttga agttcgccct ctgttcctct gtacgtctgt agcggccgtt ttcatcaaca
      481 tagtagtctt cgccttcgat accccagtag cagagcttct ggacatcttc gcggagcatg
      541 aaatcaagga attcaagtgc gcgctcgaca tccttgcact tcacgctgat acccattccg
      601 ttgccgccta cgataccggc gctcttgtac ctgttgtacg gcagagcttc gtttccatca
      661 aggctgatgc cgagaggtac ataggtacgt tcatacttgc cttcggaaat cagaacgttt
      721 tctgcgtttc cgaagttcca ctgctggtcg aacattgcca gtacacggcc tgtggagatg
      781 actgacagat agtcgtcata gttcctggag aatgtctcag gattgatcag gcctttctta
      841 tacatttcgt tgagtttctt gtaatatgcc tttgcataat ctttgttctg gtatatctca
      901 actgtgagat ccggcttgac aacgacgtcg ccttcgttac gtccgccgat gaggtgctgc
      961 ggagcattct tcagacagaa cgaacgccaa tcctgtgaat gtacttcgaa tcccagagta
     1021 ggctggccgt cgatcgtggg atgagccttg tagtaacgct cgagcaaatc gaagtaagtg
     1081 tccagattct tcggatatga ataattgtcc catgcaagaa cgtctttctg tgtccagaaa
     1141 gcggggccgt tgtaacctac ctgatatgcg gtgtcttctc cgtcgttcag ccagtattca
     1201 cggctccaga tgtccatgat gtagatcttg ccgtcagacg aaacgctacg gagtacatcc
     1261 aggtaaggtt cataatgttt ccagaggttc ggatacttgg gaagataatc gtcgagtgct
     1321 atgaacgcac ctgcgttaag gaactgtcca cgagcctgac cgggcccgat gaggtcgggg
     1381 tagtcgccgc tggcgatcat tataccgact ttctgttcca tgtcacctac gagatattcg
     1441 atctcaatgg tgacgccagt cgcttcctgg atcttcttga tgatcttgtt gtctgccgga
     1501 ggagcttcgc ccggatcggc ctggaacata gtgaacgtta tcggctcttt cttttcttca
     1561 gccggtacac tgggttctgt tcctcccgtg tcactgttag gtgttgttgc tggctggtcg
     1621 cctgatcctt tggtttcctg ctttccgcac gctgtcataa caccaaagat cataacagca
     1681 accaacagca gcgcgattag tcttactttt ttcattattt gatccccttt ctattttaat
     1741 tttttgggtt ttcaaacaat tttatctttc tattttgctc atctttcctg ctgtgttcgg
     1801 aactacggtt agttatacgt tttaatctgc gttatttaag ggctcaaagg atagctgcgt
     1861 caatttggtc aactaatttt tttgtgcaat atcactatta cggaacaatt atataataca
     1921 tttttgtatt tgtctataat tctgtataaa attcctcagc ccggaacatg aaatattgtg
     1981 cactcgtgcc ggaattcccc tgatatctct cacattcaaa tatttgtgca ttctgaccta
     2041 acattttcgt ttgcttttct ggtcggacat acctgttttg gataatagtt ggtgttatga
     2101 taaagtaatg taagaagccg gtccctcttt ttgaaatttc aaacgattta ccaaaaaccc
     2161 gcacggggac ccttcttaat gaaagcttga tcagagccga tccccggcac cttttaaaaa
     2221 actgatcgca tcttcttccg aaagcggttt gcttaccagg tatccctgca ccttgtcaca
     2281 gccgtgctct ttcagatact gaaactggat catgtcctct accccttctg cgaccgtact
     2341 gtgtcccagc ctgtgcgcca tcgagattat atcgctggtt atttctttat caggttccgt
     2401 ataaagcagc ttgtcaataa agtatttatc gattttcatg cgatccacat tcaattctct
     2461 ttgctcatgc gcaaagcttc cttgccgctg cttgcagtca atacgcaata ctcgtccagc
     2521 gtttttctta tgatcgctct ctgccgcgga acaacaatgc acagtaagta caaacacgtt
     2581 aacaactgcg ttaaacagtc cgactgctgt agaaaaactg aactgtgcct gttcaatgcc
     2641 tattttgtat acataggcag ggattatttc cgaaacggaa ataacaggaa accctgtttc
     2701 gcttcgatcc tgaacgactc gaccctctgg ctgcaagttt tccttcatta taatataacg
     2761 gatcagttgc tcatctggtt cacgaaataa ttatcatatg gttttccgct tccataagtc
     2821 ggattgttcc tgtcccacgg agacagatac actctaaatt ttataccagc tttcctgcag
     2881 gcatccgata cttccttttc caactgttga tgatcaagct gtcacctgct tggtacaact
     2941 tgtaccagac gcctgtccaa atctttcata tactgaccct ccgtagactt gtattattct
     3001 cccttgtttc ttttgatata aatgtcaaat ccccacggcg gcaaagttat gctccctttt
     3061 atctcttctc cggaaaccat gctcttcccg ccatctccta tatctattga tttctgttcc
     3121 ggtgtgaaat tcataacaaa aacatagtgg ttctcgccat cagtgcgcat ctgtgcagtg
     3181 cacccatacg gcaaattacc ttctatggcc ccggctatac ccagtttaca tgccagcatg
     3241 ccaaagaagt catcctggaa ctgttcgtca agtcttgcgg ccacaaaata tgcctctccc
     3301 tctccaaagc tgttgacagt cagtgcagga gatgactcat agaaatcctc acaataagta
     3361 ccaatgacct ttgctccttc tgtatggaca atttcacaaa aatcgcgggc ttcatacctt
     3421 gttccgttcc attccacatg ccttctttcc tcatcaaaaa ggctgtcgat ttcttcacac
     3481 caaatgccga gcactttccg aagcggcccg gggaacccgc caaggaagca gagatcgtgc
     3541 tcatcaacat aaccagtcat ataagtagct acgaatgtac cgccgctttt tacgaaatct
     3601 tcgatttttt cagccatacc gggacggagc atataaagca tcggcgctat aaccagctta
     3661 taacctgaaa gttccttcgt gctgtctata atgtcaaccg atatcccaga tttccagaac
     3721 ggtgcatagt gctccttaag ggtttcaata tatttcttgt tatacttgaa gccctggcac
     3781 atttcgaggg cccacctgtt ttccacgtca taaataatcg cgacatcaga gggctttgaa
     3841 gttcctataa tagcatccag cttttcaagg gtctcccctg tcctggctac ctcgcggaat
     3901 actcgtgtat gttccgtacc cacatggtcg acgacagcac catggaactt ttcagaagaa
     3961 ccgcgcgact tccggaactg aaagtattgc acggtgtcac tgccatgggc tactgcctgg
     4021 atactctgaa gttcgtgcag gccaggtctc ctcagtttac cgacgggctg ccagttcgta
     4081 gctgaagggc tgctttccat aagcataaac ggacgtccgt ccttgagccg cctgttcatg
     4141 tcatggacca ttgatgtaag tatcgcaata tccgcatcgc catcatcact ccgccatatc
     4201 gggtaattgt cccatgacgc aatatccagc gcttctgaga gacgatagta attaaggccg
     4261 tcatataatc ccatcatatt cgtagtacaa ggcacattcg gagtcaggcg cctgagaggt
     4321 tccatctccg ccttcatgaa atcaagggtc tgctcagtca caaaacgctt ccagtcaaga
     4381 gccagcccat ggatacttgt ttctcctatt gaagacgggg attcgatctg gctccaatcc
     4441 gtataagtat ggctccagaa tgctgtccac catgcagcat tcagcgcctc cagtgatcca
     4501 taacgttctt tcagccaatt gcggaaagcc tcctggcaca gatcacaatg gcactctccc
     4561 ccatattcat tggaaatgtg ccatacagca agcgcaggat ggtccttata tcgctcagcc
     4621 agcttcgtat tgatgttacg gactttttca cgataaacag gagaagtgta acaatgatta
     4681 tgccttcccc catggaggat cctgcggcgg tcctcattta ccctgagaac ttccggatat
     4741 ttttggctca gccatgccgg ccgcgcgccg gaaggggtcg caagcacagc agctataccg
     4801 ttttgggcaa gcaaatccat tacttcatcc agccatgaaa agtcgtaacg gccttcttca
     4861 ggttccaatg ccgcccaact gaatatgccg accgtaagcg tattgatatg agcaaggcgg
     4921 gcaaggcgca tgtcctcgct ccagatctct ttcacatcgc gccactggtc aggattgtaa
     4981 tcaccgccat ggagtatatg ggggaattta gggacgatcg gtttgaattt catgagatag
     5041 cctccttcat ataaattagc atataattat agcttacatt ttttgcgggc atttgtcatc
     5101 aggctgccat aatgcccagc taaatcagtt ttctgatgaa ttcaggcaga ttaatcagat
     5161 acaactatat ctgcctcata tggatccaat gttatgcagt ttctatatat ccttcctgtc
     5221 aacaagctta tcgccgtacc tgtcgaattt attgcacata cctgattaac tgtgtttaca
     5281 tagattacag cgccattttc aagggttctg gcaataaccc cttttggtgt caccggtcct
     5341 ttattccggt ttatgagcaa aactgcaaat gagcgattgt cggatttaca gaaagggcta
     5401 tttaccgcct gccagtcttt tgtacgtcga cggcgttatc ccgtacctct ttctgaagat
     5461 cctgccgaaa tagcggtaat ccctgaatcc gacctccatg gcgatctccc cgacagactg
     5521 ctccggatgc tgcaccaaca gctgtgccgc cttctcaagc ctcagtattt tgacatgctc
     5581 catcggagaa acattgtaac gtttcctgaa atactgtatg aagtaattgg gatgcaggtg
     5641 gacgatccct gcaagaccct ggagcgttat ggcagaacca atattgcgca ctatgtattc
     5701 gtctaccgca gcaataaaat cgtccgtgtt ttcaggcagg atacagacat aatcgacatt
     5761 gtccataaac agcctgagga tttccagaag cgccgccttc tccgaaagcg tatcgcgcgg
     5821 atctgctgag ttgtaagagc tgatcagctt ttcaaaaaca ggtacgatcg tctccctggg
     5881 tatcctgcat tttgccccgt atttggaata gacaagtttc cgtccttcat tcaggcgcag
     5941 gtcgaaatgg caccaatatt tatatacagg ccttacagga tcgtggtaat aggtgtggcg
     6001 ggtgtccgcc ggtatcagga acagttcctc aggctccggg tggtattcgt ctccttctat
     6061 tataagggtc gcgtcgccct cgatgaagta ataaaacttg ttgtagccgt acgagcagtc
     6121 cgtctcgccc cagtcgggcg cacagtaatt cacatggctg atcaggttct tgacgaatat
     6181 gttttcgaaa atatttttgc catggagcat tacagtcacc accttccgtc aagtatatcg
     6241 cacgatattc cggacaagaa tatgataaca taataaagtt aattttgtgc acactaatct
     6301 taatataaac ccttctgcct aaatgcaagt tcgatagaat ttaattgtat acagttctgg
     6361 tattaggtgt tgcaaccccg taaagctgtc gaaagaagac ataccaggta taaaaataaa
     6421 aatgccgtac acgggaatca ggcagctggc agctattgaa gagaatcgcc ggcaagcggc
     6481 tgactttcgt gcccggcacc aggtcgagga ggtacttatg cagccggaaa tcaggaaagg
     6541 tcctttcgaa ccaacctttg aatccctcag gacatttgaa tgccccgaat ggtaccgcga
     6601 cgccaggttc ggcatctggt ctcactgggg tgcgcagtct gtcccaatgt acggcgactg
     6661 gtatgcgcgg aacatctatt gtgaaggctc ggaccaatac aggtatcaca tacgccgtta
     6721 cggacatccg tcaaaattcg gctacaagga tatcgtacag ctttggaagg ctgaaaactt
     6781 cgatcctgac ggccttatgg agctatatgc tgaagcaggc gcgaaatatt tcgtggccca
     6841 ggcaatgcac cacgacaact tcttcaatta tgcctcgaag atccacccct ggaattcggc
     6901 gaacatgggg ccccataaag acatagtagg cctctggaag gaggctgctg acaaaaaagg
     6961 cctgccgttc ggcgtatccg agcacctcgg cgccacgttc agctggtacc gggtaaacaa
     7021 aggctacgac agggaaggac cgttcgcagg agtgccgtac gacgggaacg atcctgaata
     7081 tgaaagcctc tacctgccca acagggagca ttatgatccg gacaaaggcc actgggacct
     7141 ggacccgtgg tatactccca acaggtggtg gcaccagaga tggttcgaaa taattaaaga
     7201 agtgatcgat ctttaccagc ctgacctgct gtatacggac ggcggagtcc cgttcgggga
     7261 cggcatgtat gaaacaggtc tcaacgtgat cgcgcatctt tacaacacaa gcgctgcaaa
     7321 acatggagga aaaaacatgg ccgtctacac gcagaaggac cgcaggaagg agatttacac
     7381 ggtaggcgtg cttgatatag aaaggagcca gtcggaggaa ataaggcagg aaccgtggca
     7441 gaccgatacc tgcgtcggtc actggttcta tgatgtgcgg gccgcctaca aaaagcccgg
     7501 ccatgtgatc gaaatactgg tggacgtcat atcgaagaac ggcaacctgc tgctgaacat
     7561 cccccagaaa cccgacggaa cccttgatga cgaatgcctg tatatcctca gggaactggc
     7621 aaaatggaac agggtatgcg gcgaaggcgt atacggcacg aggccgttcc aggtacccgg
     7681 ggaaggccct tcacgcgtac atatcgaagg tttcagggaa gacccggtgg catggacagg
     7741 ttcagattac cgcttcacaa caaaggataa aaccatctat gccttccaga tgaaatggcc
     7801 tgggaacaac cgggcggtga taaaatcgct tggaccttcc gagaaggtca gggccgtcag
     7861 gctgctcggc tatggccagg tgccttttga acagccctac ggtacccttg tcgtcagcct
     7921 gccggataaa aagccgacag aatatgtaaa ctgcctggct ttggagatgg aatgataaaa
     7981 cggcgcctgg cgcccgggta aaagaggtgt aagcatatgg acaaaaaaac attcaaaata
     8041 gccgatgacg gtatcgatcc gaatattgtg ccatttaagg tcctgtacac aggagcgaag
     8101 atgcccggga taggtcttgg caccttcgga tcggaccggt acagcggcga ggagatagca
     8161 gccgcggtaa aggaagcgat atcgctgggt taccgccata tcgactgtgc agccgtgtac
     8221 ggcaacgagc atctgatcgg caaatccctt cgtgatatcc tggaaagcgg ccttgtaaaa
     8281 agagatgagc ttttcatcac atcaaagctc tggaacgaca tgcacggcga cggagatgtg
     8341 ctcattgcct gcgcaaagac actgaaagat ctgaagctgg aatacctgga tctctatctt
     8401 gtgcactggc ccttccccaa ccatcacgct cccggggtcg gagccgattc aagggatccg
     8461 catgctaagc catatatcca cgaggattac atgaaggtat ggcgccagat ggaacggctg
     8521 gtggatgccg ggcttgtcag gcatatcggc acttccaaca tgaccgttcc aaaactggaa
     8581 ctgctcctgc gggacgcaag ggtacggcct gccgtcaacc agatggaact ccatccgcat
     8641 ttccagcagc cggaactttt ccggtattgc cttgatcgag gcatcgtacc ggtaggctat
     8701 tgcccggtcg gatcgcctaa acgtcccgac cgcgacagga cacccgagga cactgtggat
     8761 atagaggatc cggtgatcgt caggatcgcc gaaagccaca acgtacatcc cgcgataatt
     8821 tgcatcaagt gggctgtgca gagaggacag gtcccgatcc cgttctcgac cgacaggaat
     8881 catttgcgca gcaacctgct ctgcgcagtc accgacccgc tgactgacga ggaaatgcgc
     8941 aagatcgaaa aaacagaccg caactgccgc ctgatcaagg gacatgtctt tttatgggaa
     9001 ggcgcgaaag actggactgc cctctgggat atcgacggag taataaacgg agcaacagaa
     9061 gagctcgatc ggtaatacat ccggcatata ccggtaatat aagtgttagt cctgaactgc
     9121 aatataaagg gggattgtta tgggaaacct gatgaaagct gcttatttgc ctggcaacag
     9181 cacagtggtg ctcaaagaag tggagatccc ggaaccgggg tatgggcagg tactgatccg
     9241 tacaaaagcc tcaactatct gcggcagcga tatccgcgcc atataccacg agcatctcgg
     9301 gaaaggcccc gaaggctatc agaacaagat cgccgggcac gaaccggccg gacagatcgt
     9361 aaaatgcggt cctggtatga ggagattcaa ggaaggagac agggtcatcg tgtaccacat
     9421 ttcaggatgc ggtctgtgcg tcgactgccg tatgggttac atgataagct gtacgagtcc
     9481 gctgcgggca gcatacggct ggcagaggga cggtggtatg gcgccgtata tcctg
//
LOCUS       DAOSCC010000086         9365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4823_length_9365_cov_4.094522, whole genome shotgun sequence.
ACCESSION   DAOSCC010000086 DAOSCC010000000
VERSION     DAOSCC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4823_length_9365_cov_4.094522"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1818)
                     /locus_tag="PLG72_06065"
     CDS             complement(358..1818)
                     /locus_tag="PLG72_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80397.1"
                     /translation="MNDEAKAFKLHMGPAPCAVDLGDGSERGEYVCQDYILDKLGRPH
                     RSINLMYCYYPLDKGWPTRASRLKTDKNVTFQWDYAYDDYFPYTGGIGGSTDGEPFIS
                     MKDVRRHGQDVTLTMTVDCAVPDEHLIQIARELRPFGRLLFRINHEATGNWFAFSRRY
                     TYQQVADFYVRFHNIVKQEAPHIKTILCIGGEAVPGSSEMPYEKEFREAVRAADIWSG
                     DYYLALNWGWPFTIAERGGKSHRRYDIRERFELNRFVYERFCYLNDGVPKPLVISELN
                     ADGDVTGPYEQAEMVKEFYYMFRDEKADWMKGITMYQFRDRGRLGLELEDPNCPENGI
                     KQPLFDTYREIIHDPWFYPAMETGEAVELPVRLRWGGFEDADGLAVPVWLEKSPVFCE
                     VTFEEDLNVVFEFNGKWFYKKPGVKTVDLMPAFYEKPFRGGQEVMLRLFAPPADGVND
                     PSQGEDWEINYYTRVTRLPEFRIRYEPIELRARKEG"
     gene            complement(1894..4260)
                     /locus_tag="PLG72_06070"
     CDS             complement(1894..4260)
                     /locus_tag="PLG72_06070"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80398.1"
                     /translation="MRYGWFDDANREYVIEKPDTPMSWVNYLGTAEYCGIISNNASGY
                     GFYKSPKSARMLRFRFNSIPMDRPGRYIYIRDDEDGDYWSASWQPVGKPLDSYRTVCR
                     HGLGYTKFESEYKGIRSDYRVFVPVDKPVEIWEVEVENRSGRERELSLFTYAEWCFWN
                     MNQDLTNFQYILYTCRMGYENDVVDYSIRLWPISEPKAFMASTLPVVSFETDRDEFIG
                     RYRHEGNPIAVERGYNANTIAVGGNPCASVQNKIKLEPGEKACAVYIVGIGDAKTFGQ
                     ECKKLYSDRANVEKEFEKVKQYWVKRLSAYTCSTPSPLVNSMVNIWNQYQCHTTFNWS
                     RSASFNEAGGRHGLGYRDTNQDTLGVMHSIPGLCRGKIIDLLKGQISNGSAMHGLQPL
                     TWKQGPHNVPPEQFIFSDDHLWLLLSVPAYIRETGDMAFLDVTAPYCDEGEATVYGHL
                     KQALEFSWGKRGPHGLLLGLAADWNDCINLKGKGESIWSTFLYYRALTEFIELAERAG
                     QKADAEHFAKYRAEIAWNIDKYAWDGKWFLRGYLDSGKKLGGQESDQCKIFINSQTWA
                     VVSGAASAEKGMQAMDSLKQYLATEHGIVKNYPAYREPDTEVGAITSFPPGLKENAGI
                     FCHANTWAIIAECMLGRGDRAFEYYMAFLPAAKNDIADVYTMEPYVYSQFITGKEHPY
                     KFGRARNSWLTGTASWSFVAVSQYILGVRASYDGLVVDPCIPHDWDGFKVRRIFRGKT
                     FDIEVKNPDRVSKGVVSMAVNGKKLGGTLIPFDVMQGSNKVEVVLGRK"
     gene            4458..5072
                     /locus_tag="PLG72_06075"
     CDS             4458..5072
                     /locus_tag="PLG72_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ80399.1"
                     /translation="MDNPTLKLEKRDAVGGGKAKRLLEKGLVPAVVYGKEFGSTPVQV
                     DRSELRKFLRANGRNTVFKTEFAQEQDFSLIIKNIQYHPVNREIIHLDFQKVDPQEIV
                     QMEIPVRVTGEEIFTRTGNSVLHQLNTITVECPAGSIPEYAVADISGLKPGQSFTVAD
                     FLFPSGVKVLTDPSKVVITIKGHRPEQASEGERETQIPVPATES"
     gene            complement(5098..6828)
                     /locus_tag="PLG72_06080"
     CDS             complement(5098..6828)
                     /locus_tag="PLG72_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80400.1"
                     /translation="MYRSTETGKRLIRLAKAFNSDGFEIVRTAAYNGRLGITAESREA
                     VNYDTGRPKRAYYVEGTPYEMGWLMGKMAEEEIARMMDYTDRVVFSFIGSKVLEKMKL
                     VQSILIKIIHKLSKKTWQQLPPEITEEVRGIYDGCRSRNPKTRVDMQRLIVLNTGIDI
                     LCSVVYSGKFFKRALEGIEPEDFDIPMMCNAFVAGGESAGKGCFFGRDFTFPSAGVFQ
                     DTCAYILYSPVTPAGGYGDAHDNDPAPEILAERRDTPIPFVNIAAPGMVGSIAAMNLN
                     GVAVGVNMSPGANCDPEGIGINSLLMTRLCVQYGRSAEDSAALIAGLPKGVSWLYITA
                     DGTGRSCIAEAGASWPEPDFTQYADEEYRTILPDMEFIKSHAAVPYRNGAMFRWNDYK
                     YPQEYLAFNRQLWDYYNSRKKTGKAIYPDAFGKTGYINRPGDRNCPSTFYFAPQRENS
                     DDLLVASNHYIIPEMRYFAMNRWTQRVIGKRADDIQWRYDELNRQIHDKLAEKGGIGL
                     EDAKELISFLSPYGRFRDYYADNPRSSDGKEIRIEGCVSAFDLKNRVVESHYGYYCDE
                     WVRISPARYF"
     gene            complement(7020..7214)
                     /locus_tag="PLG72_06085"
     CDS             complement(7020..7214)
                     /locus_tag="PLG72_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HOJ80401.1"
                     /translation="MYALRNLSYLKRRIDKLRMELEQKIKSAGTDDICMDEKLYRLSA
                     KLDVLINKYIKEQQKIQQSM"
     gene            7406..8251
                     /locus_tag="PLG72_06090"
     CDS             7406..8251
                     /locus_tag="PLG72_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80402.1"
                     /translation="MKALHQKTLLDADFPMNIFFTDVTEFPPHWHEVVELVYITEGSL
                     TIGVNNEVYTMEPGDIVLVSGSDVHYFVPIPNQVNRLIVHFDMAYFESAYPEAKNKRF
                     AIVHMKKGQISDNGVDVHELLVEQLDEIISEYKEQRPAYKLVLRARLFDVLSILIRNV
                     PMQPYSPQERSRQQKRLGRLENVFRYVEENYSSPISLEEIASVANFSEYYFTRFFKEA
                     TGMTFGRYLNNYRVEKAAQFLRNGDDSITEVVFKSGFGSIKTFNRVFKQIKGCSPSAY
                     KKDNF"
     gene            8427..>9365
                     /locus_tag="PLG72_06095"
     CDS             8427..>9365
                     /locus_tag="PLG72_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80403.1"
                     /translation="MGYKNFVSALYAPVGNLNAIKDLQSFAEDFRYIEKHVHIDKIYL
                     ETFRGFEYIDREKILLLKNFFEAKGIKVSGGITTSDAKWHEYGFTSLCYSDSGHREKI
                     KAVAEMTASIFDEIILDDFFFTNCKCEACIQAKGGSTWSEFRLRQMKEVSRELIVGPA
                     RKVNPNVNMIVKYPNWYDCFSETGYNLRDEPAIFDMIYTGTETRDPLYTQQHLQKYLS
                     YFIMRYFENVKPGKNGGGWFDPYECRGNLNYYVEQAYLTLFAKPREVTLFCLGSLLDE
                     RFRIFTPLAGYAYEAVDRFLGELGEPVGTACYIPYHS"
BASE COUNT         2237 a         2559 c         2244 g         2325 t
ORIGIN      
        1 aactgtgaca ggttttacga tgcgcttctc gatcagttcg gccacatcat ggacctgaac
       61 ttcgccaagc ctgaaagcaa cctgctttat catgtgacat ccgatgtgga agacgcattg
      121 aaatacattg atacatacaa accgttcgtg tttgaggaca agtggctgac ataaggcgca
      181 gtaagcggac ggcccgagat acggttgcct gtccggtgaa atgcagccgg tccggccact
      241 cggcaaaggt gctcaaaaag cccggcttca tcagcgaaaa gccgggccct gcatttcgga
      301 tatgttttcc ggacccgctg ctggtccgtt tcggcataaa cctgcctggt aaaatactta
      361 gccctctttc cgggccctaa gttcaattgg ttcatacctg atgcggaact ccggcagcct
      421 ggtcacccgg gtgtaataat tgatttccca gtcttctccc tgcgacggat cgttcactcc
      481 gtcggccgga ggcgcaaaaa gccttaacat gacctcctgc ccgcctctga acggcttttc
      541 atagaacgcc ggcatcaggt cgacggtctt cacgcccggt tttttataga accatttacc
      601 gttgaactca aatacgacat tgaggtcctc ctcgaacgtg acttcgcaga acaccgggct
      661 cttctccagc cagaccggta ccgccagccc gtcggcatcc tcgaagcctc cccatctaag
      721 cctgacaggc agttccacgg cttcacccgt ttccatggca ggatagaacc acggatcgtg
      781 tataatttcc ctgtaagtgt cgaacaacgg ctgctttata ccgttttcag ggcagttcgg
      841 gtcctccagt tcaaggccga gacgtcccct gtccctgaac tggtacatcg ttatgccctt
      901 catccagtcg gctttctcat ccctgaacat gtagtagaat tccttcacca tttcagcctg
      961 ttcgtacggt cccgtcacat ctccgtcggc attgagttcg gagatgacga gcggctttgg
     1021 cacaccgtcg ttcaggtagc agaacctttc atatacaaac ctgtttagct cgaatctctc
     1081 ccttatatcg taccgcctgt gggatttgcc gccgcgttct gctatggtga acggccagcc
     1141 ccagttcaat gccaggtaat aatcgcccga ccagatgtcg gccgccctta cagcctcgcg
     1201 gaactctttc tcataaggca tctcgctgga tcccggtaca gcttcgccgc caatacaaag
     1261 gatcgtcttt atatgaggcg cctcctgttt tactatgttg tggaagcgca catagaaatc
     1321 agcgacctgc tgatatgtat atctcctgct gaaggcgaac cagttgcccg tagcctcatg
     1381 attgatccgg aacagcagcc tgccaaaagg cctgagctct cttgcaatct ggatgaggtg
     1441 ctcatccggc acggcacagt caacagtcat ggtgagtgta acatcctggc cgtggcgcct
     1501 tacatccttc atcgaaataa acggctcgcc gtccgtactc cctcctatgc cgccggtata
     1561 cggaaagtaa tcgtcatatg cgtaatccca ctggaaagtc acgtttttgt ccgtcttcag
     1621 cctgctcgcc cgtgtaggcc agcctttgtc cagcggataa tagcagtaca tgagattgat
     1681 gcttctgtga ggtctgccca gcttgtccag tatgtaatcc tggcatacat actcgccccg
     1741 ctcgctgccg tcacccaggt cgacagcgca cggagcaggc cccatgtgca gtttgaaagc
     1801 ctttgcttca tcgttcattg acagcttcat cctttcgttt ttccaggtag tcgttcagca
     1861 tctgaaaaaa gtattcatta tgcccaaaag taatcatttg cgtccgagca cgacttcgac
     1921 tttattgctg ccctgcatga cgtcaaacgg tataagcgtc ccgcccagct ttttgccgtt
     1981 aactgccatg gaaaccactc ccttcgatac cctgtccgga tttttgacct cgatatcgaa
     2041 agtcttgccc ctgaatatcc tgcgtacttt gaaaccgtcc cagtcatgcg ggatgcatgg
     2101 gtcgacaacg agcccgtcat atgaagccct gactccgagt atgtactggg acacggccac
     2161 aaagctccag gaagccgtgc ctgtcagcca cgagttcctc gcgcgcccga acttgtacgg
     2221 gtgctccttg cccgtgatga actgtgaata tacataaggc tccatcgtgt acacatcagc
     2281 aatgtcgttt ttggctgccg gaaggaatgc catgtaatat tcgaacgccc tgtcgcccct
     2341 gcccagcatg cactcggcga ttatagccca tgtgttcgcg tggcagaata tgccggcgtt
     2401 ctccttaagc cccggaggga atgaagtgat ggcgccgact tctgtgtcag gctccctgta
     2461 tgcggggtag tttttcacta taccgtgctc cgtggctagg tattgcttca ggctgtccat
     2521 tgcctgcatc cccttttcgg cagaagccgc gcccgacact acggcccatg tctggctgtt
     2581 tataaatatc ttgcactggt cgctttcctg ccctccaagc tttttcccgc tgtcgagata
     2641 gcccctcagg aaccatttgc cgtcccatgc atatttatcg atgttccagg ctatttcagc
     2701 cctgtatttc gcgaaatgct cagcatcagc cttctgtccg gctctttccg caagctcgat
     2761 aaactcggtg agggcgcggt aatacaggaa cgtgctccag atgctctcac cttttccttt
     2821 caggttgatg cagtcgttcc agtctgccgc aaggcccagc agcagcccgt gaggccctct
     2881 cttgccccat gagaattcaa gcgcctgttt cagatggccg taaaccgtcg cctcgccctc
     2941 atcgcagtac ggcgccgtta catccaggaa tgccatgtcc cctgtttccc tgatgtatgc
     3001 gggcaccgac agcagcagcc acaggtggtc gtcggagaat atgaactgtt cgggcggcac
     3061 attatgcggt ccctgcttcc aggtgagggg ctgaagcccg tgcattgccg aaccgtttga
     3121 tatctgtccc ttcagcaggt cgattatctt gccgcggcaa agcccgggta tcgaatgcat
     3181 gacgcccagc gtgtcctggt ttgtatcgcg gtagccaagt ccgtgtcgtc ctcccgcttc
     3241 attgaatgaa gccgacctgg accagttgaa agtcgtatgg cactggtact ggttccatat
     3301 gttgaccatc gagttcacca gcggggaagg cgtggagcat gtgtaggcag acagcctctt
     3361 tacccagtac tgctttacct tctcgaactc tttttccacg ttggcccggt cggaatacag
     3421 ctttttacat tcctgcccga aggttttggc gtcgccgatg ccgactatat aaacggcgca
     3481 ggccttttct ccaggttcca gctttatctt gttctgcacc gatgcgcacg gattgcctcc
     3541 gacagctatg gtgttcgcat tgtaacctct ttccacggcg atcggattgc cctcatggcg
     3601 gtaacgccct atgaactcgt ccctgtcggt ttcaaagctc actaccggca gcgtggatgc
     3661 catgaaagcc ttgggctcgg atatcggcca gaggcgtatg gaatagtcca ccacatcgtt
     3721 ctcatagccc atcctgcatg tgtaaagtat gtactggaag ttggtcaggt cctggttcat
     3781 gttccagaaa caccattcgg cataagtaaa cagggacagt tccctttccc tgcccgatct
     3841 gttctcgacc tcgacttccc agatctcaac aggtttatcg accggcacga ataccctgta
     3901 atcagacctg atccctttgt attcgctttc aaacttcgtg taccccagcc cgtgccggca
     3961 aacggtcctg taactgtcca gcggcttgcc gaccggctgc caggatgcag accagtaatc
     4021 gccgtcttca tcgtccctga tataaatgta tcggccggga cggtccattg gtatgctgtt
     4081 gaaccggaat ctcagcatcc tcgccgattt cggcgattta taaaagccat atccgcttgc
     4141 gttgttggag ataatgccgc aatactccgc agtaccaagg taattcaccc aggacatcgg
     4201 tgtgtccggc ttttcaatga catattccct gttggcgtcg tcgaaccagc catatcgcat
     4261 aaggtgcccc tccttcaaaa tctgcatatt tcggatatcg agctgcactt agattatttt
     4321 agcataaaaa gcaagcaaat catattcttt ttaatcctgg cctggacgat cctggccgcc
     4381 cggctgccgg aagacgttta gaaaatgatt atctgtcaat aataacctta cgaaaaagca
     4441 ttttttcgga ggtaagcatg gataacccga cactgaaact ggaaaaaaga gatgcagtgg
     4501 ggggaggcaa agccaaaagg ctgcttgaaa aaggtctcgt ccccgctgtt gtttatggaa
     4561 aggagttcgg atcaactcca gtccaggtcg acagatccga actcaggaaa tttctcaggg
     4621 ccaatggcag gaatacggtc ttcaaaaccg aattcgccca ggaacaggac ttttcgctga
     4681 tcattaaaaa catacagtac catcctgtaa acagggagat catccatctt gatttccaga
     4741 aggtcgaccc gcaggaaatc gtgcaaatgg agatacctgt aagggtcaca ggcgaagaga
     4801 tattcacaag gaccggcaat tcagttctgc accagctgaa tacgataacc gtcgaatgtc
     4861 cggccggcag tatccccgaa tatgccgttg ctgacatctc aggcctgaaa cccggccaga
     4921 gctttacagt tgcagacttc ctgttcccat ccggcgtgaa ggtgctgacc gatccctcca
     4981 aggtggtcat cacaataaag gggcaccggc cggaacaggc atcggaaggg gaaagggaaa
     5041 cgcaaatacc tgtaccggct acggaatcat aggctgcaca tacccgcaat cgccggctca
     5101 gaaatacctg gccggcgata tccttaccca ttcgtcacag taatatccat aatggctttc
     5161 caccaccctg tttttcagat cgaacgcgga tacgcagccc tcgatccgga tctccttccc
     5221 gtcgctgctt ctcgggttgt cggcataata atccctgaat ctgccgtaag gcgaaaggaa
     5281 actgatcagt tccttcgcat cctcaagacc gatgccgccc ttttcagcca gtttgtcatg
     5341 tatctggcgg ttcagttcat catagcgcca ctgtatgtca tcggcccttt tgccgataac
     5401 gcgctgtgtc cagcggttca tggcgaaata gcgcatctcg gggatgatat aatggttgga
     5461 tgcaaccagc aggtcgtcgc tgttttccct ctggggcgca aagtagaacg tggacgggca
     5521 gttccgatct ccgggcctgt tgatatagcc ggttttcccg aaagcatccg gatatattgc
     5581 tttgccggtt ttcttcctgc tgttgtaata atcccaaagc tgacggttga atgccaggta
     5641 ctcctgcgga tatttgtaat cgttccatct gaacattgca ccgttccggt aaggtactgc
     5701 cgcatggctc tttataaact ccatatcagg caggatcgtc ctgtactcct cgtcggcata
     5761 ctgcgtgaaa tcaggttccg gccacgacgc gcctgcttca gcgatacagg atcgccctgt
     5821 gccgtctgcc gttatgtaca gccaggatac ccctttcggc agtccggcta tcaacgcggc
     5881 ggaatcttcc gcgcttctgc cgtactggac gcaaagcctt gtcataagca gcgagttgat
     5941 ccctattccc tcggggtcgc agtttgcgcc aggcgacatg ttaacgccca ctgcaacgcc
     6001 gtttaaattc attgccgcta tgctccccac catacccgga gccgctatat tcacaaaagg
     6061 tatgggagta tcccgccgtt ctgcaagtat ctccggggcc ggatcgttgt catgagcgtc
     6121 accgtatcct ccagcaggcg tcaccggcga atacaggata tatgcgcagg tgtcctggaa
     6181 aacaccggcg gaaggaaaag tgaaatccct gccgaaaaaa cagcctttgc ctgcgctttc
     6241 cccgccggcg acaaatgcat tgcacatcat cggtatgtca aaatcttcag gttctatgcc
     6301 ttccaatgcg cgtttgaaga atttccccga atataccacg gaacagagta tatcgatgcc
     6361 ggtgttgagc acgatgagcc gctgcatatc cacacgggtc ttcggattac ggctcctgca
     6421 tccgtcatat atgccccgta cctcttccgt tatttccggc ggcagctgct gccaggtctt
     6481 tttggacaac ttatggatga tctttatcag gatgctttgg accagcttca tcttttcaag
     6541 cactttgctt cctataaacg aaaaaacgac cctgtcggtg taatccatca tcctggcgat
     6601 ttcttcctcg gccatctttc ccatgagcca gcccatttca tatggcgtac cctcgacata
     6661 atatgccctc ttgggcctgc cggtatcgta attgactgct tccctgcttt cagcagtaat
     6721 gcccagcctg ccgttatatg cagcagtcct tacgatttca aatccgtcgc tgttgaaggc
     6781 cttcgcaaga cgtatcagcc gcttccccgt ctccgtgctc ctgtacaagc gaaacactcc
     6841 ttcagcaaaa accggtgccg caaccgcatt gcatccaccg gcatatgaat tcccgtacca
     6901 tattatgcgt ggaatattga aagggaaccg gcaaacagtc cggaacaaaa agcaactgga
     6961 aatgaaccgt tatgcttgat cacccttttg ggtataaata tgtgatcttt tttgcacgat
     7021 cacatcgatt gctgtatttt ctgctgctct tttatatact tgttgatgag aacgtcaagt
     7081 tttgcactga gcctgtataa cttctcgtcc atgcagatat catccgtccc ggcggatttt
     7141 attttttgtt ccagttccat acgcaattta tctattctcc gctttaaata agataaattc
     7201 cgcaaagcat acaccccgta aaaacttatc gatgaaacac aacagaacaa gtatatataa
     7261 actggaaaat ataatcaata tataaaaata aaaaaatata ctcttcagaa cgaggttatg
     7321 taaaattttg tggtatatta aaataaggtc gaacagtgga tgaccactgg ccaaatacaa
     7381 atccatacgc acaaggaaga tcagattgaa ggcgctgcat cagaagactc tgcttgacgc
     7441 agatttcccg atgaacatct tttttaccga tgttacggag ttcccgcctc actggcatga
     7501 ggtggtggaa ctggtttaca taaccgaagg aagcctgacg ataggcgtga acaatgaagt
     7561 atacacgatg gagcccggag atatagtgct cgtttcaggc agcgacgtgc actacttcgt
     7621 accgatcccg aaccaggtga acaggcttat cgtgcatttc gacatggctt acttcgagtc
     7681 tgcatacccg gaagccaaaa acaagcggtt cgccatagtg cacatgaaaa agggacagat
     7741 atcggacaac ggcgtggacg tgcatgaact ccttgtggaa caactggacg aaatcataag
     7801 cgaatataag gaacagcggc cagcttacaa actggtgcta agggcaaggt tgttcgacgt
     7861 cctgtcgata ctgatacgga acgttccgat gcagccctat tcgccgcagg aacgctccag
     7921 gcagcagaag cgccttggca ggctggaaaa cgtcttccgc tatgtcgagg aaaactactc
     7981 ctcaccgata tcgcttgaag agatcgcttc agtggccaac ttcagcgagt attacttcac
     8041 aaggtttttc aaggaggcca ccggtatgac gttcggcaga tacctgaaca attaccgggt
     8101 ggaaaaggca gcccagttcc tgaggaacgg cgacgactcg atcaccgaag tagtgttcaa
     8161 atccggcttt ggcagcatca aaacattcaa cagggtcttc aagcagataa aaggctgttc
     8221 tccgtcggca tacaaaaagg acaatttctg agacctggca ggcaattatt gacgagcagg
     8281 ctgtacggct atgctttaga atagtaaccg gcagtctctt tttacatgct ttcgggagct
     8341 tcatcacggg aatcccgaaa tgccgggccg cacatgtgtc cggataacag gcggaagact
     8401 attgcaaagg gacggggagg atgaatatgg gatacaaaaa ttttgtttcg gcgctgtatg
     8461 caccggtggg caacctgaac gcaataaagg atctgcagag ttttgcggaa gatttcaggt
     8521 acattgaaaa gcatgtgcat atcgacaaga tataccttga gacgttcagg ggctttgaat
     8581 atatcgaccg ggaaaagatc ctgcttttga aaaatttctt cgaagcaaaa gggataaagg
     8641 tatcgggcgg catcaccaca tcggacgcca aatggcatga gtatggtttc acatcccttt
     8701 gctactctga cagcgggcac agggaaaaga taaaggcagt cgctgaaatg acagcatcga
     8761 tattcgatga gataatcctc gacgacttct tctttacgaa ctgcaaatgc gaagcctgca
     8821 tacaggccaa aggcggcagt acctggtcag agttccggct ccgccagatg aaggaagtat
     8881 ccagggaact gatcgtcggg cccgccagga aagtcaatcc caatgtcaat atgatagtga
     8941 agtatcccaa ctggtatgac tgcttcagtg aaacaggcta caatctcagg gacgaacccg
     9001 caatattcga catgatctat acgggcacgg aaaccaggga tcccctctat acacagcagc
     9061 atctgcagaa gtatctgagc tacttcataa tgaggtattt cgaaaacgtc aagcctggta
     9121 aaaacggcgg cggatggttc gatccatatg aatgcagggg caacctcaac tattatgttg
     9181 aacaggcata tctgacactg ttcgcaaagc cccgtgaagt caccctgttc tgtctcggct
     9241 ccctgcttga cgagaggttc aggatcttta ctcccctggc agggtatgca tatgaagccg
     9301 tggaccggtt cctcggcgaa ctcggagaac ctgtgggaac tgcatgctat atcccttacc
     9361 attcc
//
LOCUS       DAOSCC010000087         9353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4828_length_9353_cov_4.006668, whole genome shotgun sequence.
ACCESSION   DAOSCC010000087 DAOSCC010000000
VERSION     DAOSCC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_9353_cov_4.006668"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLG72_06100"
     CDS             <1..841
                     /locus_tag="PLG72_06100"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ80404.1"
                     /translation="GHMVLVNALINEARMSGLESVVYTFMKHPENIIRKNLFTPLLTT
                     VGKRIQILGETRLDHVFFDEFDEDFSRMEPEAFVKDILADRLGACVVVAGFNYRFGYM
                     GRGDSELLKELGKKYGIRVIIIPAVSIGPEIVSSTAIRGYVLKGDMEKAALLLGRYYS
                     IMGKVLDGKRVGRKIGFPTANLRPEDYLILPGNGVYLTKTLYEGVLYNSVTNVGVNPT
                     FGETGAISVETHILNFGKDIYESNIEVFFLKKLRDEVKFGSGGELSAQISLDIKTATE
                     YFG"
     gene            945..1685
                     /locus_tag="PLG72_06105"
     CDS             945..1685
                     /locus_tag="PLG72_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80405.1"
                     /translation="MIIRMEGIGKIYDTGKVQVEALKNVNLCIDKGEFVAVMGPSGSG
                     KSTLMNIIGCLDRATSGHYELDGVNISDLDDTELARIRNKKIGFVFQSFNLLPKMNAL
                     QNVELPVIYAGAGKKERREKAVEALNKVGLLDRMDHRPNELSGGQKQRVAIARALVND
                     PTIILADEPTGNLDTASGEDIMAVFQELNREGVTIVLVTHEPDIAEHTRRIVRFRDGM
                     LISDEKVEDPIDARDEIIKLKMKTQAAG"
     gene            1805..2527
                     /locus_tag="PLG72_06110"
     CDS             1805..2527
                     /locus_tag="PLG72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ80406.1"
                     /translation="MDQEVAGTRPGFFRRLIWLFFSPGRLMDELAEKPRVLFWMIFGS
                     LAAVIPFVVRMSLYEDMIRKSVAASSEYLESFGIEMTPEMIEASLPSSIIAGLAGMPF
                     ETAGALALLALLFFIIMRIMGGEGKFKAYLSVVVHANIISILYNLMLTAVSYYSGSLH
                     QTAALTSLASLVSPDVTDPYIYGLLAGIDVFMIWRFAVMAIGFTAVSKLKKKQVYIAT
                     AVIYIIHIALTIYNMPAALKMM"
     gene            2565..3827
                     /locus_tag="PLG72_06115"
     CDS             2565..3827
                     /locus_tag="PLG72_06115"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ80407.1"
                     /translation="MSKKVIIGSILVISTAVLVTAGAMRAAGSTGSGAVSVQAAAVVK
                     GDLSSWIYADGVIEEVEKSEVYFDTPMKVTKVMISEGQEVKRGQQLMEVDMSELDSRL
                     ETLKSNRRTQQIALDSKAADAEVERALNSLKAAERNYNDAQKAYEDNKALYEANAISK
                     SELDMSERQFREAESGVKNARLAYNAAVESRNSSRKTAEENIKVTDIQISDLEKKIND
                     INDLCKAAIDGVVTSVNIQEGAYTSSMQPAYRIINPDKLRIRAQVSEYDIKNVVVGQK
                     VRVTGDAIDKDTEITGTVQIISPVATTAMTSAGSETVVEVIIGVDDTRGVLKPGLNVT
                     CEIVTVDKSGVVLVPMEAIKPDKDDNLMVFVVDTETNRIEQRRIEAGINSDMNVEVID
                     GLEEGDLVVIDPQPNYSDGMKVKVNRQY"
     gene            3863..5074
                     /locus_tag="PLG72_06120"
     CDS             3863..5074
                     /locus_tag="PLG72_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80408.1"
                     /translation="MNFSESFKQALDSLKSNKLRSFLTMLGIVMGVFSVITIMALGSA
                     TENYMNEQFERIGANTISITYKNANVDKRDWLTLKDMEYVKEAAPEIKNIATYIVRSG
                     TVRVDDKTKDAQVFGVSSQYKNFAIIEMKYGRFINEFDVTKRAKFAVVDEYFVRKLFK
                     EDRDVTGETITLKTPSGTVSIKIIGVMSSGENMFEGIMESDLIPVMIYMPITTIQQLY
                     PNSEKLASIEVSVVEKDKLREVGKRIVQYLEMKHGSEDVYIAQNSADIQKAFSDVLGV
                     VSSILLVIAVITLVVGGIGIVNILLVSVTERIREIGIRKALGAQKKDIILQFLTESIL
                     MTGCAGLVGIFLGIVAGGIISALIDIPPVVDLKVITLAFLGSVALGIVFGVYPAKRAA
                     DLDPIESLRYE"
     gene            5706..7172
                     /locus_tag="PLG72_06125"
     CDS             5706..7172
                     /locus_tag="PLG72_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ80409.1"
                     /translation="MIVRINQNRTPLFDAVKKYIDDNVIPFHVPGHKQGRGLREMVEY
                     IGEKALRMDVNGMKDLDFANNPTGVIMEAQKLMAHAYGAENAYFLVNGTTSGVQAMIL
                     SACDPGSRIIIPRNAHRSAIGGIILSGAMPVYVLPEINRELGITMGISAEKVREAIRK
                     NPHAKAVFVVNPTYYGMTSDIKSIVRTAHMHDMSVIIDEAHGAHMMFHDDFPLTAMEV
                     GADMSAVSVHKTAGSLTQSSVLLTRGALIPQERIRQSLGLTFTSSASYLLMCSLDIAR
                     KQLATRGPELLGEALELARWAREEINRIPGLYAFGRELTGREGCYDFDETKLGINVRQ
                     TGYTGYQVEALLREKFNIQVEMSDLYNILAIVSIGDRREDVTALVNALRQLSLHKGGS
                     MKVNDFEIPESPEMIVTPRDAFYNSKKTVRLEDSAGEIAGEMVMAYPPGIPVICLGER
                     ITRDVIDYIMLLKQEKCELQGTYDPDVEYIRVLGSSSA"
     gene            complement(7229..7642)
                     /locus_tag="PLG72_06130"
     CDS             complement(7229..7642)
                     /locus_tag="PLG72_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ80410.1"
                     /translation="MVVLLALFGMICWGIAPVFAKAALKDIDPTAGLVLRTIFAASVV
                     SGWVIISGNFSKVSSIPAATWWLIATEALLATLVGDLAYYAALKKGDVSLVTIVMSSS
                     PLVTILCSAVFLGEQITLMRLVGAILVIAGIILIV"
     gene            7943..>8360
                     /locus_tag="PLG72_06135"
     CDS             7943..>8360
                     /locus_tag="PLG72_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ80411.1"
                     /translation="MVSGPERHMPWANEKEFIEAVARHKTYVMLGGYKTVLKDPLPGE
                     EYNVHLAADMLAGMVIRPGEIFSQNNAIGPYTENRGFREGPTYLGTMVTKTVGGGVCK
                     VASTLYNVAVLSNMKIVERHNHTMPVPYVPYGQDATV"
     assembly_gap    8361..8370
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8371..8711
                     /locus_tag="PLG72_06140"
     CDS             <8371..8711
                     /locus_tag="PLG72_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80412.1"
                     /translation="RFMNTTGSDILIWAKGVDNALYMAFYGSKPAPEVEWIHEVLETT
                     KAPVLYRINSSLDEDDRRLLMEGMDGAVVRSWVRITEGGNVQVKYMGLSRYQPMPHLF
                     EKGPDRRNAH"
     gene            8797..>9353
                     /locus_tag="PLG72_06145"
     CDS             8797..>9353
                     /locus_tag="PLG72_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ80413.1"
                     /translation="MKIDRSGSNSRSFRVFLAGQGFLGLAESVRFIAVTILIHDITGS
                     GISAATGTALSALPGILASPFAGVIGDRSGERGLLVLIDVLRSLAVLLFLFAGNITQI
                     YLLIILISVSDVFYNPSRRKYVLRLTGKKGALKANSLLTGAGGAAWLAGPLFAGFLAD
                     RYGPAPSILLSSVCCLITALFTIFSA"
BASE COUNT         2562 a         2117 c         2590 g         2074 t
ORIGIN      
        1 ggggcacatg gtgcttgtca atgcccttat caatgaggca aggatgagcg gcctggaatc
       61 cgtggtttat acattcatga aacatcctga aaacataatc aggaaaaacc tgttcactcc
      121 attgctcaca accgtcggga aacgcataca gatccttgga gagaccaggc tggaccatgt
      181 cttcttcgat gaattcgacg aggatttctc aaggatggag cccgaggcat ttgtaaaaga
      241 tatactggct gacaggctcg gggcgtgtgt ggtagtggcc ggattcaatt acaggtttgg
      301 ctatatgggc cggggcgaca gcgaactgct taaggaactg ggaaagaagt atggcataag
      361 ggtcataatc atacctgcag tgagtatagg tccggagata gtgagcagta cggcgatacg
      421 cgggtacgtt ctgaaagggg acatggaaaa ggcggccttg cttcttggca ggtactattc
      481 gataatgggg aaagtgctgg acggaaagcg tgtcggaagg aagatcggct tcccgacagc
      541 caacctgcgc cccgaagatt atctaatatt gccaggcaat ggcgtctacc tgacaaagac
      601 gctttatgaa ggagtactgt acaacagcgt tacaaacgtg ggggtcaacc cgaccttcgg
      661 agagaccgga gcaataagcg ttgagacgca catactcaac ttcgggaaag acatttatga
      721 aagcaacatc gaagtattct tcctgaaaaa gctcagggat gaagtaaaat tcggaagcgg
      781 aggcgaactg tcagcgcaga tcagccttga tataaagacg gcaacggaat atttcgggta
      841 gacatctcag taaatatttc tatattaaga agacaaatcc atgaagaaaa gcgtataaac
      901 gttgggaagt catataggta aaagctgtag aggaaggtat tgccatgatc atcaggatgg
      961 aaggaatagg gaagatatat gataccggca aagtacaggt ggaagccctg aagaacgtta
     1021 acctttgcat tgacaaagga gagtttgtcg ctgtcatggg tccgtcgggc tcagggaaat
     1081 cgaccctgat gaatataatc gggtgcctgg acagagccac cagcgggcat tatgaacttg
     1141 acggcgtcaa catatcggat ctggatgata ctgaactggc aaggataaga aacaaaaaaa
     1201 tcgggttcgt gttccagtca ttcaatctgc taccaaagat gaatgccctt cagaatgtcg
     1261 aacttcccgt gatatatgcc ggcgccggca aaaaggagcg cagggaaaag gctgttgagg
     1321 cgcttaataa ggtagggctg cttgacagga tggaccacag gcccaatgaa ctgtccggcg
     1381 gacagaagca gagagtggcg atagccaggg cccttgtgaa cgacccgacc atcatacttg
     1441 ccgacgaacc aacaggaaac ctcgatacgg catcgggaga agacataatg gctgtattcc
     1501 aggaactgaa cagggaggga gtcaccatcg tccttgttac acatgagccc gatatagcag
     1561 agcataccag gcgcatagtc aggttcaggg acggaatgct gataagcgat gagaaggtgg
     1621 aggatcccat cgacgcaaga gacgagatca taaaactgaa aatgaaaacg caggctgccg
     1681 gctgacggca gcagtttgcg gaattatgcg gcaacagtca ggtttccggt gcggaggctg
     1741 tttgatacac agtaaaaaat gagaggaaga ggatgatatc ttgtctgata ttcaggtgcg
     1801 gaatatggat caggaagtag ccggcacaag gccgggattt ttcagaaggc tcatatggct
     1861 gtttttttcg ccgggaaggc tgatggatga actggctgaa aagccgaggg tactgttctg
     1921 gatgattttc ggatctttgg cggcagttat tccattcgtt gtcaggatgt ccctgtatga
     1981 ggatatgatc aggaaatcgg tcgctgccag ttccgaatac ctggaatcct ttggcatcga
     2041 gatgacgccc gagatgatag aagcatcatt gccaagcagt ataatcgccg gacttgccgg
     2101 catgcctttt gagacagcgg gtgcacttgc cctccttgct ttgctgtttt tcatcataat
     2161 gaggataatg ggaggggaag gcaagttcaa agcatacctt tctgttgtgg ttcatgccaa
     2221 cataataagt atcctgtaca atctgatgct gacagccgtt tcatattatt cgggaagcct
     2281 gcatcagacg gctgccctga caagcctggc ttcactggtt tcgcctgacg taacggatcc
     2341 gtatatctac gggctccttg cgggcatcga cgttttcatg atatggagat tcgccgtgat
     2401 ggccataggc ttcactgctg tcagcaaact gaaaaagaaa caggtctata tcgccacggc
     2461 tgtaatatac atcatccata tagccctgac tatatacaac atgcctgcgg cacttaagat
     2521 gatgtaactg cggtccgatg acagacattt gtggaggtaa tgcgatgagc aaaaaggtaa
     2581 ttatcggaag catacttgtg atatcaactg ccgtcctggt cacggccggg gcaatgagag
     2641 ccgccggttc gaccggttcc ggcgcggtaa gcgtacaggc cgctgcggtg gtaaagggag
     2701 atctttcctc ctggatatat gcggacggtg ttatagaaga agtagagaag tcagaggttt
     2761 atttcgatac gccgatgaaa gtgacaaaag tcatgatatc tgaggggcag gaggtaaaac
     2821 gcggccagca gttgatggag gtggatatga gcgaactgga ctccaggctc gagacactga
     2881 agagcaacag gaggacacag cagatagcgc tggattcgaa agctgccgat gctgaggtcg
     2941 aaagggccct caacagcctg aaagccgccg aaaggaacta taatgacgcc caaaaggcat
     3001 atgaggacaa caaggctctg tacgaagcca acgcgatttc aaagtccgaa cttgatatgt
     3061 cggaaaggca gttcagggaa gccgaatccg gcgtaaaaaa tgccaggctc gcttataatg
     3121 cggctgtcga aagcaggaac agctccagga aaacggcaga agaaaatata aaggtcacgg
     3181 atatccagat atccgatctt gagaagaaga taaatgacat aaatgattta tgcaaagccg
     3241 ccatcgatgg tgtcgtcact tcagtgaaca tacaggaagg agcatacacc agcagcatgc
     3301 agccggctta caggataata aaccctgaca aactccggat aagggcgcag gtaagcgaat
     3361 atgacatcaa gaatgtggtt gtcggccaaa aagttagggt gaccggagat gcgatcgata
     3421 aggacacaga gataacggga actgttcaga tcatttcacc cgtggcaacc acagccatga
     3481 catcagccgg aagcgaaacg gtggtggaag tgataatcgg cgtggatgac accaggggcg
     3541 tactgaaacc gggactcaac gttacctgcg aaatagtaac ggtggataag agcggagtcg
     3601 tgctggtgcc gatggaagct ataaagcctg ataaggacga caacctgatg gtgttcgtcg
     3661 tcgatactga aacgaacagg atcgagcagc gcaggataga ggccggcatc aactccgata
     3721 tgaacgttga ggtaattgat ggccttgaag aaggagatct tgttgtcatt gaccctcagc
     3781 ccaattacag cgacgggatg aaagtcaaag tgaaccggca gtattgacag ggcagcccac
     3841 aggaaagaag tgaggtacgc agatgaattt ctccgaaagc ttcaaacagg ctcttgacag
     3901 cctgaaatca aataaactcc gctcgttcct taccatgctc ggtattgtga tgggcgtatt
     3961 ctccgtcatt acgatcatgg cgcttggaag tgcaactgaa aactacatga atgaacaatt
     4021 tgagaggatc ggagcaaata cgataagcat cacctacaag aatgccaatg tcgacaaaag
     4081 ggactggctg acgctcaaag acatggaata tgtcaaagag gccgcaccag agatcaagaa
     4141 tatcgcgacc tatatagtca gaagcggcac tgtccgtgtc gatgacaaga caaaggatgc
     4201 acaggtgttt ggcgtatctt cccaatataa gaattttgct attattgaaa tgaaatacgg
     4261 ccggttcata aatgaattcg atgttacgaa aagagcaaaa ttcgccgttg ttgatgagta
     4321 cttcgtcaga aaattattca aggaagacag ggatgtgacc ggagagacca ttactctcaa
     4381 gaccccgtca ggcactgttt ccattaagat aataggagtg atgagcagcg gggagaacat
     4441 gttcgagggt atcatggaaa gcgatctgat acctgttatg atctacatgc ccattacaac
     4501 catacagcag ctttacccaa acagtgaaaa gctggcgtcc atagaggtgt cggtggtgga
     4561 aaaggataaa ctgcgtgaag tgggtaaacg gatcgtgcag tacctggaaa tgaagcatgg
     4621 cagtgaagat gtatatattg cccagaattc agccgatata cagaaggcgt tcagcgatgt
     4681 gcttggtgtc gtttcatcga tactcctggt aatcgccgtc atcaccctgg tagtcggagg
     4741 tatcggcata gtgaacatcc tgctggtgtc agtgacggaa aggataaggg agataggcat
     4801 caggaaggct cttggcgcgc agaagaaaga cataatactc cagttcctga ccgaatccat
     4861 actgatgacc ggctgcgccg gccttgtcgg cattttcctc ggcatcgtcg caggaggtat
     4921 aatctctgcc ctgatagata taccgcctgt ggtggacctg aaggtcatca cactcgcatt
     4981 tctcgggtcc gttgcgcttg gaatagtttt tggcgtttat cctgcgaaac gtgcagccga
     5041 tctcgatccg atagagtcgt taaggtatga gtagacataa ttgagccgtg ggggataggc
     5101 aatcatacat ttgggatgtt cgtggacagc tgctggccgg ctcatttgct ccaatgaggg
     5161 cgctggtcac tcgccgtccg tggctcggac ttagcgccgg cagcgtccgt gctgccggtt
     5221 gaggtgcctg gtgcctcatt cgagctgcat gacccggccg catctgtagc acttgcatcc
     5281 catcaatgcc tgattgccca tccacccacg gcttttcatg cccttatatc tgaaatgaat
     5341 acaaggatga cgcaggagat gggcaatcat acatttgggg atgttcgtgg acagctgctg
     5401 gccggctcat ttgctccaat gagggcgctg gtcactcgcc gtccgtggct cggacttagc
     5461 gccggcagcg tccgtgctgc cggttgaggt gcctggtgcc tcattcgagc tgcatgaccc
     5521 ggccgcatct gtagcacttg catcccatta atgtctgatt gcccatctcc tgcgtctttt
     5581 ttgcgcctga tactgatttc ctgacttgaa aacacttctg gcggcggagt cctgagcagg
     5641 gccatgcaca ggcccgatcc gtgcgccata ttatagacgg atgaagtttg gcgacgatac
     5701 aggtgatcat tgtgcggatc aaccaaaaca ggactccgct ttttgatgca gtcaaaaaat
     5761 atatcgacga caatgtgatc cctttccatg tgccaggaca caagcagggg agagggctcc
     5821 gtgaaatggt cgaatacatt ggggaaaaag cactccggat ggacgtcaac ggcatgaaag
     5881 accttgattt tgccaacaac ccgaccgggg tcataatgga agcacagaag ctgatggcac
     5941 atgcttatgg cgcggaaaat gcgtatttcc ttgtaaacgg caccacatca ggcgtacagg
     6001 caatgatcct cagcgcgtgc gatccgggaa gcaggatcat catcccgcgg aatgcgcaca
     6061 gatctgccat cggaggaata atactcagcg gtgcgatgcc tgtgtatgtg cttcccgaaa
     6121 taaaccgcga actcgggata acgatgggca tatccgcaga gaaggtcagg gaggcgataa
     6181 ggaaaaatcc gcatgccaaa gctgtgttcg tcgttaatcc cacatattac ggaatgacat
     6241 ctgatataaa gtccattgtc aggaccgcgc atatgcatga catgtccgtg atcatagatg
     6301 aagcgcatgg cgcgcatatg atgttccacg atgatttccc gctgactgcc atggaagtgg
     6361 gagccgatat gagcgccgta agtgtacata aaacagccgg atcgcttacc caaagctctg
     6421 tgctgctgac gaggggagcc cttataccgc aggaaaggat aagacagtcg ctgggcctta
     6481 catttacttc gagcgcttca tatctgctga tgtgttcgct ggatatagcc aggaaacagc
     6541 ttgccacgag aggcccggaa ttgctgggag aggcccttga acttgcgcga tgggcaaggg
     6601 aggagatcaa caggattccc ggcctttacg cgtttggacg tgaactgaca ggcagagaag
     6661 gatgttacga ttttgatgag acaaaacttg gcatcaatgt aaggcaaacg ggatataccg
     6721 gctaccaggt ggaagcgctg ctgagggaga agttcaacat acaggtggag atgtcggacc
     6781 tgtacaacat actggccatc gtatccatcg gcgacaggag agaagacgtc accgcccttg
     6841 tgaatgccct cagacagtta tcgcttcaca aaggcggcag tatgaaggtg aatgattttg
     6901 agatacctga aagccctgag atgatagtca ctcccagaga cgcgttttat aacagcaaga
     6961 aaaccgtgag gctcgaggat tcggcggggg agatagcagg cgagatggtg atggcatatc
     7021 ctccgggcat acctgtcata tgccttggtg aaaggattac cagggacgtg atcgactata
     7081 taatgctgct gaaacaggag aaatgcgaac tgcagggcac atacgaccct gacgtggagt
     7141 acataagagt tctaggcagc agttctgcgt gaccgtccat acgatgcctt gttccacaga
     7201 tcatgtgacc ctgtgaacca cgcggccgct aaacgataag gattatcccg gctatcacga
     7261 gtatggcgcc gaccaggcgc atcagggtta tctgttcacc aaggaaaact gctgaacaca
     7321 atatggtgac cagcggtgaa cttgacatta ctatagtaac aagtgacacg tcgcctttct
     7381 tcaatgcggc ataatacgcc aggtctccca ccagtgtcgc aagcagggct tcagtggcga
     7441 tgagccacca tgtggcagcc ggtatgctgc tcactttcga aaaattcccg cttattatga
     7501 cccagccaga aacgacgctc gcagcaaaaa tcgtgcggag gaccagaccg gctgtcggat
     7561 cgatgtcctt aagggcggcc tttgcaaaca ccggcgctat gccccagcag atcatgccaa
     7621 aaagcgcaag cagcactacc atattttttc tgctcctgtc gttttttatc attgtcccgt
     7681 tatcccttcc ataattgaca atatgtccgc agaaaccgca ttattactgg aatttattta
     7741 ttgtagtaaa tcaggcgcat actgataaaa ataagtttat ctgaaaagga gaaggtatct
     7801 ggaattgcgc aggttgatca cagttatatt tgtcacagta tgtctgtcaa ttgctcaata
     7861 cgctgccgca gagccgtgtg acagaatccg gtggcctggg gaggataaga ctggggccat
     7921 cggggaaggc ttcagtactg aaatggtatc aggacctgaa cgtcacatgc catgggctaa
     7981 tgaaaaagaa ttcatcgaag ctgtagcccg gcacaaaacg tatgtgatgc ttggcgggta
     8041 caaaactgtc ctgaaagatc ctctgccagg tgaggagtac aacgtgcacc tggcggccga
     8101 catgcttgca ggtatggtta tcaggccggg ggaaatattc tcgcagaaca atgccatcgg
     8161 cccatacacg gagaacagag gcttcaggga aggacccacc tatctcggga ccatggtgac
     8221 gaaaaccgtc ggaggaggcg tatgcaaggt cgcatccaca ctgtacaatg ttgccgtact
     8281 gtcaaatatg aagatagtgg agcgccacaa ccatacgatg ccggtgcctt atgtccctta
     8341 cggccaggac gctacagtgt nnnnnnnnnn tcaggttcat gaacacgacc ggctcagaca
     8401 tactcatctg ggcaaagggt gtggacaatg cgctttatat ggctttttac ggaagcaagc
     8461 ccgccccgga agtggaatgg atccacgaag tgcttgagac cacaaaggct cctgtgctgt
     8521 acaggataaa cagcagtttg gacgaagatg acaggaggct gctgatggag ggaatggacg
     8581 gcgcagtggt caggtcgtgg gtaaggatca cggaaggcgg caatgtacag gtgaaatata
     8641 tggggctcag cagataccag cctatgccgc atctgttcga aaagggccct gacaggcgga
     8701 atgcccattg acgacatcag caaagcaaag accgatgcat aaacccgatg tttctgaata
     8761 acattcaaat atgatcctgt tttccgggga aaaataatga aaattgaccg cagcggttcc
     8821 aacagcaggt cattcagggt attccttgcc ggccagggtt tccttggcct ggcggaatcc
     8881 gtaaggttca tagctgtaac tatactgata cacgatataa ccggctccgg catttcagct
     8941 gcaacaggca cagcactttc cgcactgccg ggtatactgg cttcgccttt tgcgggcgtg
     9001 atcggcgaca gatccggtga aaggggcctg ctcgtgctca tagatgtcct gagatccctg
     9061 gcggttttgc tgttcctctt tgcaggcaat atcacgcaga tatatctgct catcatactg
     9121 atttccgttt ccgatgtttt ctataatccg tccagacgga aatacgtgct gcggttgaca
     9181 ggaaaaaagg gagcgcttaa ggccaactcg ctgctgacgg gcgccggcgg tgccgcctgg
     9241 ctggcaggtc cgctgtttgc gggtttcctt gccgacagat acggtccggc accttcgatc
     9301 ttgctgtcat cggtctgctg ccttataacc gctttattta cgatcttttc ggc
//
LOCUS       DAOSCC010000088         9198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4932_length_9198_cov_4.541179, whole genome shotgun sequence.
ACCESSION   DAOSCC010000088 DAOSCC010000000
VERSION     DAOSCC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4932_length_9198_cov_4.541179"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLG72_06150"
     CDS             <1..1059
                     /locus_tag="PLG72_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ80414.1"
                     /translation="DWSNATGIDCGNECWHHTIEEGQIIGHSIIRRNIIKNAGVCGIA
                     GMYVKNMLIEDNLIVGTGWQRMELSWEAGGIKVHNCIDSLIRRNVIKECYGCDALWMD
                     VGNENNRVTSNLFLDGIDSREHIFIECTRDKENLIDNNIIWNVEGRYDRSKVPQEPGS
                     SGWYKTTETGYTNGYGIYLEGTDRLRIVNNLIGRCNKAGFFAKVVAFRLMPKRGGTSR
                     ENKFYNNLFYGCGEAAIILPNEHNEAEGNAYAKMPPGYLRVMYPEPEMCLDLSAWQEF
                     CGFDKAGCECDIEIDVNSEDLTMEIVFRSALPEVLADKKVATDYFGNATGEKRMPGPF
                     AALKGDRIRLNIDPRKPG"
     gene            1250..2827
                     /locus_tag="PLG72_06155"
     CDS             1250..2827
                     /locus_tag="PLG72_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ80415.1"
                     /translation="MVDIKIDRESTVPVYIQIFNGIRDMILSGAMPAGYKLPPERKLA
                     DQLGVNRSTVINAYRELKSCGLAESHVGRGTVAAGCSAGDKAVYSDTGMAGIPWDPAG
                     MDRADVPGIPPMPWDQLFSESSLRMQDTTVRDLLKLSGRKDMVLFAAGISVQGIDSLD
                     TLQRIQAEVIDEYGHTTVQHVPSDGFYPLRESIGKLSAARGITASAQDIMVLSGSQQG
                     IYLCACAFIDPGDVVFVEEPTFFSAIHIFRAQGARVVGIPTDKDGMRTDLLSVLLKRF
                     KPKLIYTIPDFQNPSGAVMNMERRQELLSLAYRNQTIILEDDPYGMLRYEGDSLPALK
                     AMDIHGHVLYLSSFSKLLFPGFRVGWISGPQQVLHRLTLLKQMVDLHTGSLNQMIIDR
                     FLREGLLEDHLEKSKNENRKRRDVMLEELEAAKLEGIKWNRPEGGLYLWCRLPDRMQQ
                     TALLTRSVDKGVTFVPGNIFYPGNTPGNYIRLNFTYPTPPEIKEGVRRLAAAYKEVRK
                     EQEKYHADDHMELGPIL"
     gene            3147..3656
                     /locus_tag="PLG72_06160"
                     /pseudo
     CDS             3147..3656
                     /locus_tag="PLG72_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            4421..7261
                     /gene="uvrA"
                     /locus_tag="PLG72_06165"
     CDS             4421..7261
                     /gene="uvrA"
                     /locus_tag="PLG72_06165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ80416.1"
                     /translation="MERNSIFIKGAREHNLKNIDVTIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDYIDGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARIGVPHCPVCGKEISQQTVDQMADAIMALPEGTRIQLLAPVVRGR
                     KGEYQKLIENARKNGYVRLRVDGEVRDINEEIVLEKNIKHTIEIVVDRLVVRPDITKR
                     LTDSIETVLNLSGGLLIVDVIGGQEMMFSQNFACTDCGISIEELTPRMFSFNNPYGAC
                     PKCDGLGTLMEIDPDLVIPDRTKSLDEGAIKVNGWNFENGDSYSRMYLNALARHYGFR
                     TSTPVNELPEHVVDIILYGTKGEKIKIEYDREYGSGSFTAPFEGVITSMERRYRETQS
                     ESMKRYYEEFMSNNPCPECSGARLKKESLAVTVGGLNIYELTCRPVSEIRDFFDNLAL
                     TERERLIAHQILKEIKARIGFLVDVGLDYLTLARSSYTLSGGEAQRIRLATQIGSGLM
                     GVMYILDEPSIGLHQRDNGRLLKTLEKLRDLGNTLIVVEHDEETMYAADYIIDMGPGP
                     GIHGGYVIAEGTVEDIKKCENSQTGLYLSGKKKIEIPEKRRMPNGKWLKIIGVRENNL
                     KNIDVGIPLGVFTAVTGVSGSGKSSLINEILYKKLAADLNKAKTKPGDHDHIAGIEYL
                     DKVINIDQSPIGRTPRSNPATYTGVFDMIREVFAGVPEAKMRGYKSGRFSFNVKGGRC
                     EACSGDGIVKIEMHFLPDIYVPCEVCKGKRYNRETLEVRYKGKNIADVLDMTVEDALE
                     FFKNIPRIYSKIETLYDVGLGYLKLGQPSTTLSGGEAQRVKLATELSRKATGKTLYIL
                     DEPTTGLHIADVHRLIDVLQRLVDAGNTVVVIEHNLDVVKTADYIIDLGPEGGNRGGY
                     VIAKGTPEEVAETDGSYTGQFLKELFRREGRC"
     gene            complement(7837..7962)
                     /locus_tag="PLG72_06170"
     CDS             complement(7837..7962)
                     /locus_tag="PLG72_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80417.1"
                     /translation="MSDRGGILGDFFNDDCAILFFILVFLFLFVGPRRFGFADPK"
     gene            8323..>9198
                     /locus_tag="PLG72_06175"
     CDS             8323..>9198
                     /locus_tag="PLG72_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ80418.1"
                     /translation="MTMCSICKENYAVVFITKIINGKQIQEGLCLSCARKQGIQPINQ
                     LIEQTGMTDDDIDNLNKQMSSFFESMDMDLENSAGTEGTQGYNRGNPFLNFLSRAFPR
                     SEENGDGTDPRELGEGKDGKGGMRTKTQEKKGLKKKKYLDMYGTNLTDLARNDKIDLV
                     IGRDREIDRVLQILNRRTKNNPVLIGEPGVGKTAIAEGLAVRIAKHQVPAKLFNTEMY
                     LLDMTAIVAGTQFRGQFESRMKGIIEEAKALGNVILVIDELHNIIGAGEAEGAMNAAN
                     ILKPALSRGEIQIIGA"
BASE COUNT         2762 a         2082 c         2486 g         1868 t
ORIGIN      
        1 gactggtcca atgcaaccgg catagactgt gggaacgagt gctggcatca cacaattgag
       61 gaaggccaga tcatagggca ttccatcatc agacgaaaca taataaagaa tgccggtgta
      121 tgcgggatcg caggcatgta cgttaaaaac atgctgatcg aggacaatct gatcgtcggc
      181 acaggatggc agaggatgga gctttcctgg gaagcgggag gcatcaaggt ccataactgc
      241 atcgattcgc tcatacgccg caatgtcata aaggaatgct atggctgcga cgcgctgtgg
      301 atggacgtcg gtaacgagaa caacagggtc acatccaatc tgtttctgga cgggatcgat
      361 tcaagggagc acattttcat cgaatgtacg cgtgataaag agaatctcat agacaacaac
      421 ataatctgga atgtggaagg acgttatgac agatcaaagg tgcctcagga gccgggttcc
      481 tcaggctggt acaaaacaac cgagacggga tacaccaacg gctatggcat ctaccttgaa
      541 ggaacagaca gactccgtat cgtcaacaac ctgataggca ggtgcaataa agccgggttc
      601 tttgcaaagg tagtggcatt ccggctaatg ccgaagcgtg ggggaacgtc gcgggagaac
      661 aagttctaca acaacctgtt ctacggctgc ggcgaagctg ccatcatact gccgaacgag
      721 cataacgaag cggaaggcaa tgcatacgcc aaaatgccgc ctggttatct gcgcgtgatg
      781 taccccgaac ccgaaatgtg cctggacctt tcggcctggc aggaattctg cggatttgac
      841 aaagcaggat gcgaatgcga tatcgagatc gacgtcaaca gcgaggacct gacaatggaa
      901 atagtgttcc gatcagcttt gccggaagtg ttggctgata aaaaagttgc tacggactac
      961 tttggcaatg cgactggtga gaaaaggatg ccaggaccat tcgctgcact taaaggagac
     1021 aggatacggc tgaacatcga tccgaggaaa ccgggctgaa cgtacagcca gccattacag
     1081 agcatatgat catcctccat gccagactat cctgaacgga tatacgaagg ttcggcagcg
     1141 ccatatttaa ttggatggta tcattttatt ggaattggat gtttatttat ttaatattca
     1201 cagtataatg tactaaatgg atgacaccaa tatcgaaaaa gagggatgaa tggtggatat
     1261 caaaatcgac agggaaagta ctgtacccgt ttatatacag attttcaacg gaataaggga
     1321 catgatcctt tcaggagcca tgcccgcagg atataagctt cctcccgagc gaaaacttgc
     1381 ggatcagttg ggcgtgaaca gaagcaccgt aataaacgca tacagagagc ttaagtcatg
     1441 tgggctggca gaatcacacg ttggaagggg tacggtcgcg gcaggctgca gtgccggtga
     1501 taaagccgta tactcggaca ccggaatggc aggcatacca tgggatccgg caggcatgga
     1561 ccgggcggat gttccgggga tccctccaat gccgtgggat cagttattca gcgaaagttc
     1621 gcttcgtatg caggacacta cggtcagaga ccttttgaaa ctttcaggaa gaaaggacat
     1681 ggttctgttt gcggcgggaa tatctgtcca ggggatcgat tccctggata ccctgcaaag
     1741 gatacaggcg gaagtcatcg atgaatatgg ccatacaaca gtgcagcatg taccgtcgga
     1801 cggcttttat ccgctgaggg aaagcatagg caaactgtca gctgccaggg ggataaccgc
     1861 atcggcgcag gacatcatgg tgctgtcggg ttcccagcag ggcatatatc tttgcgcctg
     1921 tgcatttatc gatccgggtg atgtcgtttt tgtcgaagaa cctacttttt tcagtgctat
     1981 tcatatattc agagcacagg gcgcgcgtgt cgtagggata ccgaccgaca aggacggcat
     2041 gcggacggat cttctgtcag tattgcttaa aaggttcaag ccaaagctca tttatacgat
     2101 acctgatttc cagaacccgt caggcgccgt aatgaacatg gaaaggcgcc aggaactgct
     2161 ttcccttgct tacaggaacc agaccatcat actggaggac gacccttacg gcatgctcag
     2221 atatgagggt gacagtttgc cggcacttaa agccatggac atacacgggc atgtcctgta
     2281 tctcagttct ttctcaaagc tgctgttccc tggcttcagg gtcggctgga tctccgggcc
     2341 gcagcaggta ctgcaccgcc tcacattgct gaagcagatg gtggacctcc atacaggcag
     2401 tctgaaccag atgatcatag acaggttttt aagggaaggt ttactggaag atcatcttga
     2461 gaagtcgaaa aacgaaaaca ggaaaagaag ggacgtaatg ctcgaagagc ttgaggccgc
     2521 caaactggaa ggaataaagt ggaacaggcc cgaaggcgga ttatatcttt ggtgccgtct
     2581 gccggaccgg atgcagcaga cagcattgct gacacgttca gtggacaagg gagtgacatt
     2641 tgtgcccggc aatatctttt accccggaaa tacgccggga aactacatcc ggctgaattt
     2701 cacatatccg acaccgccgg agatcaaaga aggagtcaga aggctggcag cagcatacaa
     2761 agaagtccgg aaagaacagg agaaatatca tgccgatgat catatggaac ttgggcctat
     2821 actgtgaaaa gtatcaggat acagagctta ctgccgcaat aaaatttatt accacagcat
     2881 cattactcat cgcaaaaata agaatcaaag gtcgatcaga acgccattca taatacagga
     2941 atcaaccggg ctgaggtcat aaagagcctt aatttgcaga tacagtcagt tgggtcggat
     3001 gcatgattga taaaacatgc aggcagctgc gaaaatatgc agacgttttc cgcctggtcg
     3061 gcaaatgccg aaattttggg tcagtgcgta atttttttca agacaacatg agaactcttg
     3121 aaatagcgtg aaaggagcag acgaacatgg tcaaatatgc tgatcggatg gaatgtgtca
     3181 gggcttcgga tataagggag cttctcaaag tcacagagaa acctgaaatg atatcgtttg
     3241 ccggaggttt gcccgcacct gagcttttcc cggtggagcg gctgaaggaa gtgacgatga
     3301 gtgtacttga gtcatgcggc gcagaagctc ttcagtatgc aacgactgaa gggtttggcc
     3361 cgctcagggc aaaaatagcc gaaaggatga acaaaaaata cggcacctgc ctgactgcca
     3421 aaaatatcct gatagtcagc ggctcacagc agggacttga ttttacgggg aagatattcc
     3481 tcaatgaggg tgatgtggtc ctttgcgaga gtcctacgta ccttggagca ttaaacgctt
     3541 tcaatgtata caggccgaag ataatcgaag tacctacaga caacgacggg atgcttcttg
     3601 ataaactgga agagatcctt gaatcggttg acaggataaa gcttatatac acgattacga
     3661 tgcagttagg ttcacagtaa tcaaatacga agcaaacaga tctggaacgg gcagatatga
     3721 agcaagccaa tgcgatgctt gtaaatctgt ggccggcaga ctcaaagcaa atagatgcca
     3781 gaacaggtaa acataatgta tctaaaaatg aactgtgcaa aaattcaact tttgggatac
     3841 aatttatggt aataaatagc ttatgtaaac ctggagatta cataatcaca gtatcatttg
     3901 cattatgggc gtcatcatct tcaaaaacag atcgaacggc gcccaaaagt tgattttttg
     3961 aacattatct gctcgatttt gaagtttcac ttatacatca cgtaccttga tacacctcat
     4021 acgttacaaa catcatatat gacatacgca tcatatacat cacacgcata tcatctcatc
     4081 tcatctcatc tcgtctaatc acatcacaca cgtctcatct catctaatct catctaatct
     4141 aatctaatct cgtcttgtca caccacacca cacatctcat catatctaat ttaaaaatgc
     4201 tactctgaaa aaaacaaaaa caacatcgaa caaatgtttg caatttgttg atctatcgga
     4261 tataatatca tagaaaaaaa acaatgccat tgattagtac agcgtcttct ggtaatatgt
     4321 ttatccggcg ctgtaaccgg ccaggcttat taagcggcag ctggctgtat ggatccggca
     4381 gtcttcaaca gccgccaaaa tacagaaata ggtgcatagt atggagagaa acagcatttt
     4441 catcaagggt gcgagggagc acaatcttaa gaacatcgac gtgacgatac caagggacaa
     4501 gcttgtcgtc atcacgggcc tcagcggctc aggcaagtcc tcgctggcat tcgacacgat
     4561 atatgccgaa gggcagaggc gctatgtgga atcgctgtca gcatatgcaa ggcagttcct
     4621 gggacagatg gaaaagccgg atgtcgatta tatcgacggg ctgtcgcccg caatttccat
     4681 cgatcagaag acgaccagca ggaacccaag gtccacggtg ggcacggtaa ccgagatata
     4741 cgattacctc aggctcttat atgcccgcat aggagtgccg cattgccctg tctgcggcaa
     4801 ggagatatcg cagcagacgg tcgaccagat ggcggacgcg atcatggccc tgccggaggg
     4861 taccaggata cagcttcttg cgcctgtcgt aaggggcagg aagggcgaat accagaaact
     4921 tatcgaaaat gcgcggaaga atggctatgt caggctgcgc gtagacggcg aagtgcggga
     4981 tataaatgaa gagatagtcc tggaaaagaa cataaagcac accatagaaa tcgtggtgga
     5041 caggctcgtc gtaaggcctg acataaccaa aaggctgacc gattccatcg agacggtgct
     5101 gaacctgagc ggcggcctcc tgatagttga cgtgataggc gggcaggaaa tgatgttcag
     5161 ccagaatttt gcctgcaccg actgcggtat cagcatagag gaactgacgc ccaggatgtt
     5221 ctctttcaac aatccgtacg gcgcatgccc gaaatgcgac ggtctcggga ccctgatgga
     5281 gatcgacccg gacctcgtca tccccgacag gacgaagtcc cttgatgaag gcgcgataaa
     5341 ggtaaacgga tggaacttcg aaaacggcga cagctattcg aggatgtatc tgaatgctct
     5401 cgccaggcac tacggattca ggacgtccac acctgtcaac gaactgccgg agcatgtagt
     5461 ggacatcatc ctctatggga ccaaaggaga aaaaatcaaa atagaatacg acagggaata
     5521 tggcagcggc tcattcacag cgccgttcga aggcgtcatc acaagtatgg agcgccgcta
     5581 cagggaaaca cagtccgaga gcatgaagcg ctattacgag gaattcatga gcaacaaccc
     5641 ctgtcccgaa tgcagcggcg caaggctcaa aaaggaaagt ctcgcagtaa ccgtgggagg
     5701 attgaacatc tacgaactga catgcaggcc tgtcagcgag ataagggatt tcttcgacaa
     5761 cctggccctt acggagaggg aacgtctcat cgcacaccag atccttaagg agatcaaagc
     5821 caggatcggc ttcctggtgg atgtcgggct cgattacctt acgctggcca ggtcgtcata
     5881 cacgctgtcc ggaggggaag cccagaggat caggctggcg acacagatag gctcaggcct
     5941 gatgggggtc atgtatatcc tggacgagcc gagcatcggc cttcaccagc gtgacaacgg
     6001 cagactgctc aaaacgctgg aaaaactgag agacctcggg aatacgctga tagtggtgga
     6061 gcatgacgag gaaacgatgt atgccgccga ctatataatc gatatggggc cggggccggg
     6121 tatccacgga ggctacgtga tagcagaagg aactgtcgaa gacataaaga aatgtgagaa
     6181 ctcgcagaca ggcctttacc tgagcgggaa aaagaagata gagatacccg aaaagagaag
     6241 gatgccgaat ggcaagtggc tcaagataat aggagtgcgc gagaacaatc tgaaaaacat
     6301 cgatgtcggg atacccctcg gggtattcac ggcagtgacc ggtgtatcag gttcgggaaa
     6361 gagttcgctg ataaacgaga tattatacaa aaagcttgcc gccgacctga acaaggcaaa
     6421 gacgaaaccg ggagaccatg atcatatcgc cggtatcgag taccttgaca aggtcataaa
     6481 catagatcag tcgccgatag gaagaacgcc caggtccaac ccggcaacat acacaggtgt
     6541 tttcgacatg atcagggaag tctttgccgg agtacccgag gcaaaaatga gggggtacaa
     6601 gtcagggagg ttcagtttca acgtaaaggg tggccgctgc gaagcgtgct cgggagacgg
     6661 catcgtcaag atagagatgc acttcctgcc cgacatttac gtcccgtgcg aggtgtgcaa
     6721 gggcaaacgg tacaacaggg agactctgga ggtcaggtac aaggggaaga acatcgccga
     6781 tgtgctcgat atgacagtgg aagatgcgct ggagttcttc aaaaacatac cgaggatata
     6841 cagcaagatc gagacgctgt atgacgtcgg acttggttat ctgaagctgg gacagccgtc
     6901 gacgaccctg tcgggcggcg aggcccagag agtcaagctc gccaccgaac tgtcgagaaa
     6961 ggccaccgga aagacgctgt atatactgga cgagccaact accgggctcc atatcgctga
     7021 cgtgcacagg ctgatcgacg tactgcagcg cctagtggat gccggaaaca cggtcgttgt
     7081 gatagagcat aacctcgacg tcgtaaagac agcggattat atcatcgatc tcggaccgga
     7141 aggcggcaac aggggcggtt atgtgatagc aaagggaacg ccggaggagg tagctgaaac
     7201 tgatggctcg tacacgggac agttcctgaa agaactgttc aggcgtgagg gcagatgctg
     7261 aagaggaccc gaagacgtcc cggccaaagc atgaagacaa ctgcagccag gagtataaag
     7321 gcagatgttg acatgagaat taagataaat actgccggaa atttgatggg cagatgttga
     7381 caggaatgaa aagacaaata ctgcgtccaa gcggcagatg ccggcaggag tgcaaagaca
     7441 aatgctgccg ggagtccgag aggtatatgc cggcaggaat gtaacgacaa atgctccaat
     7501 gagcccgagc ggcatatgca tacaagagta caaagacaaa cactgccagg agtccaagga
     7561 gcagatactg acagcggcac aaagacaaac actgccagga gctaaggcaa atgccgtaag
     7621 aagcgtgaag gctgaagaac aatataacaa aaggtgcagg cgcgttccgg ctgtatatcg
     7681 tggttttatc ggtacaactg ccgtttcgcg ccggctttct gcatagtccg aaaatccgaa
     7741 taaaagctcg aaacatcgag cttttattcc ggaatatata gcatggagac tcttctggta
     7801 tattcgggca actgcttcat tccatggctc tccagcttat ttggggtctg caaacccgaa
     7861 tctgcgcggg cctacgaaca agaacaggaa tacgagaatg aagaacagaa tggcgcaatc
     7921 atcattaaag aagtcgccga gtatacctcc tctgtctgac ataaaaacac ctcctcatgt
     7981 acttgcgcaa tcatgataaa cttgagccta tcgctaatat aaatatattc ccaggcgccc
     8041 caatgggtta caaaaacaga gcaaaaaggt ttcggcaaag tagaccgtac attgcaaatt
     8101 gtgttatact tattgaatgg atgaaatccg gaggaaattc ttcgggatat gttagaaaca
     8161 ggtgaaatac gggaagaaca attccaggat acattaacgt accaggcatg aaaacgaaac
     8221 ctgcagcagt tgaaacctgg atacagcgaa gctaagcagc ataagggcag gacaacccgg
     8281 gcacggtttg cgccatggca gcacgaaagg agttgatcgc atatgaccat gtgctcgata
     8341 tgcaaggaaa actatgcagt agtttttata acaaaaataa taaacggaaa gcagatacag
     8401 gaggggctct gcctttcatg cgcaaggaaa cagggcatcc agcccatcaa ccaactgatc
     8461 gaacagaccg ggatgacgga tgacgatatc gacaatctca acaagcagat gagcagcttt
     8521 ttcgaaagta tggatatgga ccttgagaac agcgccggga cggaagggac acagggatac
     8581 aacaggggca atccgttcct gaacttctta agccgcgcgt tcccaaggag cgaagagaac
     8641 ggcgacggca ccgacccgag ggaactcggc gaaggaaaag acggcaaggg cggcatgcgc
     8701 accaagacac aggagaaaaa aggacttaaa aaaaagaagt acctggatat gtacggtacc
     8761 aatctgaccg atctggcgcg gaacgacaag atagacctcg tcataggcag ggacagggag
     8821 atagaccgtg tattgcagat actcaacagg aggacgaaaa acaatcctgt gctcatcggc
     8881 gagcccggag tgggcaagac agccattgcc gaagggcttg ccgtaaggat agcgaagcac
     8941 caggtacctg caaaactgtt caacaccgag atgtacctgc tggatatgac ggcaatcgtt
     9001 gccggcaccc agttcagggg gcagtttgaa agcaggatga agggcattat agaggaagcg
     9061 aaggccctgg gcaatgtcat cctggtcatt gacgaactcc acaatataat aggagcggga
     9121 gaggccgaag gggcaatgaa cgccgccaac atactcaagc ccgccctttc caggggagag
     9181 atccagatca tcggggca
//
LOCUS       DAOSCC010000089         9152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_4961_length_9152_cov_4.515884, whole genome shotgun sequence.
ACCESSION   DAOSCC010000089 DAOSCC010000000
VERSION     DAOSCC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4961_length_9152_cov_4.515884"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..3531
                     /locus_tag="PLG72_06180"
     CDS             112..3531
                     /locus_tag="PLG72_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOJ80419.1"
                     /translation="MRKVLSLTLSVILLLGMLLFPTAAFAAEAGPAPLPKAGDVISGF
                     RVTETGEMDLVNAKTVLFEHEKTGAKLYYIQNKDIEKSFTIAFRTPAVDNTGVNHILE
                     HVNLHGSRKYPINDIVFVLMNQTYSSFVNAMTAPTYTMYPVSSMSEDQLLKLADVYLD
                     SVYNPLVYQEKNVFLREGWRYEMQDAGAPLTINGTVYNEMKGSMSNITTAAYFNTMKT
                     LFPGSPQANIFGGDPGKIRDLTYEQLIETHKKYYHPSNSLMILYGDVDYTRFLKLIND
                     EYLSRYDKQETNIDYGIIKPFSKKAEARYPFPVAASAVTKNGAQIDYAFVLNDITMED
                     IHGISIIAAVLNDDSSPLKKAFSEKKTGGSMYVQLSMNTVQPTLIFTAVSADESKAGE
                     FSALVDECLRDIVKNGFDKELMDAMISRDLLDSSIITEGQHLGVGLSQSISLIWANRD
                     DLSFYSDLIRNIKKLADKLDTGYYEDLARKYILDNDHAALVTTYPEAGLAEKQEEDLR
                     KYLADLKASMSADEIEKTVNDTKAFNEWNSRESGEDVQDIIKDLQAVKAADLPVEAKE
                     RELEETKGPNGERVITVTADVGETGITGVMFDTSAVPAEKLHYLNLLSDLLGRLGTGK
                     YTREQLSKLTMRHLESARFSVGTISSKDPDVFTPVLATSWLGLMGEYGDQVDLLKEIV
                     LNTDFSDSDTVLSIIRENIANMKQSISAEPLQLLMSRSQALLSGSSKYANYVSGLEYY
                     DFLVQLEQLMQEQPEAVMAELESVYRLVVNRTNMIVAFAGNESGIGVFKDKINTLINA
                     LPAEKIVPQDYSALPGPAKREAISLDTAVQYNMLSSSHDRMGVEYNGKYIPIILMLQD
                     TYLTPLLRYQNGVYTAMASYGSKVVMLISYRDPNIKETFDVFAGIPGFLRNAAITQDE
                     LDRYILSAFGSGTLPSGELNGAYNYIMDYLSGWSVEDTLRVLNEVKSVTVEDLDDAAD
                     MFEKLIETGIYSTAGSASKLKENEDIFESVIAFGQQTDADEAITTAQFIELVLQGIQE
                     PVEAAKQQGLLVGDGKGNYMENEPLTKERLAVFIARIAAMNGIQLDGADVDIADYDSI
                     SPWARESVEALVGLGIMGLDENDSFNPKDTVTKSDVDSVLNALIMSLVAAQ"
     gene            3805..4287
                     /locus_tag="PLG72_06185"
     CDS             3805..4287
                     /locus_tag="PLG72_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80420.1"
                     /translation="MRLRNVLAAMAVAVSLLLAACVGSTPIEKYMNEDIAGIGVEKNM
                     EEAGTMLEEILGECAEKLENGEEVDWDEVEQKVDPVLAGMQETRDKVAQLEITDEDVK
                     EINSYLVSTYDKLISGYEKMIAGFRDMDDELINEGSDDLTGAQEDILKWYELIEEAIK
                     "
     gene            4725..>9152
                     /locus_tag="PLG72_06190"
     CDS             4725..>9152
                     /locus_tag="PLG72_06190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOJ80421.1"
                     /translation="MIAFVDTEIGFLKKDVLDIGCVRDDGGQFRSGVISAFIDFLKGS
                     EYVCGHNIINHDMKYIGDAVRAAGITEVIDTLYLSPLMFPARPYHKLLKDDKLQTDDI
                     NNPLNDSMKARDLFYDEVEAFRQTDDTLKRIYYLLLKDQKEFRSFFRYLGYTEHGDGN
                     REDRDCEAGREAVQYEEISEQQAEELETLIRTRFILRICENADLSGMIMNSPVELAYC
                     LSLINTGDRYSITPPWVLKNYPAVDRLLYLLRDKPCVTGCAYCNEALDIYSGLQNFFG
                     FESFRTYAGIPLQEQAVRAAIDQKSFLAIFPTGGGKSVTFQLPALMSGINTRGLTVVI
                     SPLQSLMKDQVDNLEKLNITEAVTINGLLNPIERAKAIERVENGSACILYIAPESLRS
                     KTVENLLLGRKVVRFVIDEAHCFSAWGHDFRVDYLYIGDFIRNLQEKKRLDEPIPVSC
                     FTATAKQKVIEDIREYFREKLSIEMELFTSDAVRTNLHYKVLPRETDDEKYNTIRDLL
                     SENDCPTIIYVSRTRRAYDLAQRLTADGFEARAYHGQMDVNEKTENQNAFMNGKVRIM
                     VATSAFGMGVDKKDVGMVIHYEISDSLENYIQEAGRAGRDESVDADCYVLFNDEDLNK
                     HFILLNQTKISIKEIQQIWRAIKNITKQRMRVSRSALEIARQAGWDDSISNIETKVRT
                     AIAALEEAGYLKREQNVPRVYATSILAPNAREAIEKINASPRFDENQKVKAARIIKNL
                     ISAKHWKTPELGEEPESRVDYISDILGIPKEEVIEIIYLLRDEKILANTKDMTAYIKK
                     KRQGNGSLDILKSHVQIENFLLGIYKNRAEAYNIKKLNEQAVLSGCAEATVSRLRTII
                     NIWAITKWIKRHYDTRSNVHLKLRGLISCEDLRAKLEKRHELSQFIIEYLYRKCGDEK
                     DVGEGTKSGEEIPVEFSVVELKQEFESEIRLFSTEVSLAEVEDALFYLSRIGALELEG
                     GFLVKYNAMMIERLERDNRRQYRLEDYQELEQFYQNKIQQIHIVGEYARKMMEDYQAA
                     LQFVDDYFKLNYSSFLNKYFKGSRQDEIRRSITPEKFKKLFGELSPTQLKIINDKDSK
                     HIVVAAGPGSGKTRILVHKLASLLLMEDVKHEQLLMLTFSRSAATVFKKRLLKLYGNA
                     ANYVEIKTFHSYCFDLLGKPGTLEASDNIIREAVEKIRRGETGPGRITKTVLVIDEAQ
                     DMDEDEYELVCALMEQNEEMRVIAVGDDDQNIFAFRGSSSEYMKKLITEKNAVVYELA
                     ENYRSKANIVKFTNEIAKKIPHRLKRTPIIPVQKDNGVIKIVRYKSNNLITPLVDDIM
                     STDLSGTTCVLTATNDEAVKVAGLLVNRGMPAKLIQSEEGFKLYDLQEVRYFMGLLDV
                     DNPSPVIGADDWEEAKRKLKGRFGGNRQSGSLLSGLTANPNLELCLNMIKNFEEANPK
                     LKFRSDLETLIRESRLEDFYEERGETILVSTMHKAKGREF"
BASE COUNT         2650 a         2010 c         2462 g         2030 t
ORIGIN      
        1 aggtgtctcc tttaatttta cattatatgt tagcgctttt gctcatttat agtttgattg
       61 ttatggctgg atctgccgca tcatatcatc gaaaaggaga gttgggatac catgagaaaa
      121 gtactatcgt taacacttag cgtaattctg cttctaggca tgctcctgtt tccgacggct
      181 gcgtttgccg cagaagccgg accggcgccg ctcccgaagg cgggcgatgt catctcgggc
      241 ttcagggtga cggaaacagg ggaaatggac ctggtgaatg caaaaaccgt cctgttcgag
      301 catgagaaaa caggcgcaaa gctctactac atacaaaaca aggacattga gaaatcattc
      361 actattgctt tcaggacgcc tgcagtcgac aacacgggcg tcaaccacat ccttgaacac
      421 gtgaacctgc atggttcaag gaaatatccg ataaatgaca tagtgttcgt acttatgaac
      481 cagacgtaca gttcattcgt aaatgcaatg acggctccca catatacgat gtatccggta
      541 tcgtccatga gcgaggatca gcttctgaag ctggctgacg tatacctgga cagtgtctat
      601 aaccccctgg tataccagga aaagaatgtt ttcctcaggg agggctggag gtatgaaatg
      661 caggatgccg gcgcccctct taccatcaac gggacagtct acaatgagat gaagggcagc
      721 atgagcaata tcactaccgc cgcatacttc aacaccatga aaaccctgtt ccccggcagc
      781 ccgcaggcca atattttcgg aggagacccc gggaaaatca gggacctgac atatgaacag
      841 cttatcgaaa cacacaagaa gtattaccat ccgtcgaatt cactgatgat actttacggg
      901 gacgttgact atacgcgttt cctcaagctg ataaacgacg aatacctcag caggtatgat
      961 aagcaggaaa caaacatcga ttacggaata ataaagccgt tcagcaaaaa ggcagaggca
     1021 agatacccgt tccctgtcgc ggcttccgcg gtcacaaaga atggggccca gatcgattat
     1081 gcgtttgtac ttaacgatat caccatggaa gatatccatg ggatctccat cattgccgca
     1141 gtacttaacg atgactcgtc tccgctgaag aaagcattca gtgaaaagaa aacaggcggc
     1201 agcatgtatg tccaactgag tatgaatacg gtacagccga cgctgatttt cacagccgtc
     1261 agtgctgatg agagcaaggc aggggaattc agtgcattgg tggacgaatg cctgcgcgat
     1321 atcgtaaaga acgggttcga caaggaattg atggatgcga tgatatccag ggatctgctc
     1381 gattcgtcga tcatcacgga agggcagcat cttggtgtcg gcctgtcgca gtccatcagc
     1441 ctgatatggg caaacaggga cgatctcagc ttttacagcg acctgatcag gaatatcaaa
     1501 aaacttgccg acaaactgga tacgggctat tacgaggacc tggcaaggaa gtacatcctt
     1561 gacaatgacc atgcagcgct ggtgacgacg tatcctgaag ccgggctggc tgaaaagcag
     1621 gaagaagatc tcaggaaata cctggcggat cttaaagcgt caatgagcgc agatgagatc
     1681 gaaaagaccg taaacgatac gaaggccttc aacgagtgga acagccgcga atccggcgag
     1741 gatgttcagg atatcataaa ggacctccag gcagttaaag ccgcagatct gcccgtcgag
     1801 gcaaaagagc gcgaactgga agaaacgaaa gggccgaacg gcgagagggt cataaccgtg
     1861 actgcagatg tcggcgaaac cggcattaca ggcgtcatgt tcgacacatc ggctgtgccc
     1921 gcggaaaagc tgcattacct gaatttgctc tccgacctgc ttggaaggct cggaaccggg
     1981 aagtatacca gggaacagct gagcaagttg accatgaggc atttagagag tgcgagattc
     2041 tcggtcggca cgataagctc caaagatccg gacgttttca ctcctgtact ggccacatcc
     2101 tggctggggc tgatgggtga atacggtgac caggtggacc tgctgaagga aatcgtactc
     2161 aatactgatt tttcagacag cgatactgta cttagcatta taagggaaaa tattgcgaac
     2221 atgaagcagt cgatcagcgc tgagcccctt cagctgctta tgagccgcag ccaagcgctt
     2281 ttgagcggca gcagcaagta cgcgaattat gtatccggac ttgagtatta cgatttcctg
     2341 gtacagctcg agcagttgat gcaggagcag cctgaagccg tgatggcaga gcttgagtct
     2401 gtatacaggc tggtcgtgaa caggacaaac atgatagtgg cctttgccgg aaatgaaagc
     2461 gggatcggtg tgttcaaaga caaaatcaac acgctgataa atgcgctgcc ggccgagaaa
     2521 atcgtcccgc aggactactc ggcacttccg ggacctgcga agagagaagc catatccctc
     2581 gatacggcgg tacagtataa tatgctgtct tcaagccatg acaggatggg cgtcgaatat
     2641 aacggcaaat acataccgat tatattgatg ctccaggaca cttacctgac accgttgctc
     2701 aggtaccaga acggcgtcta cactgccatg gcaagctacg gcagcaaggt cgtgatgctc
     2761 atctcctaca gggacccgaa cataaaagaa acatttgacg ttttcgcggg gatcccaggg
     2821 ttcctgagga atgccgcgat cacacaggac gaactggaca ggtatatcct gagtgcgttc
     2881 ggttccggaa cacttccgtc aggagaactg aacggcgctt ataactatat catggattac
     2941 ctgtcgggct ggtccgtcga ggacaccttg cgggtactga atgaggtcaa gtctgtaaca
     3001 gtggaggacc tggatgacgc tgcggatatg ttcgaaaaac ttatcgaaac cgggatatac
     3061 tcgacagccg gcagtgcgtc caaactgaag gaaaacgaag acattttcga atcggtaata
     3121 gccttcggac agcagacgga tgcggatgaa gcgatcacga cagcccagtt catcgagctt
     3181 gtgctgcagg gtatccagga acctgttgag gccgcaaaac agcaagggct gctggtcggc
     3241 gacggcaaag ggaactacat ggagaacgag ccgctgacga aagaaaggct tgccgtgttc
     3301 attgcaagga tagcggcgat gaacgggatc caactggacg gggcggatgt ggacatagca
     3361 gactatgaca gcatcagccc gtgggcaagg gaaagcgtgg aagcgctcgt cggcctgggc
     3421 atcatgggac tggacgaaaa cgacagtttc aacccgaagg acaccgtgac caagtccgat
     3481 gtggactctg tcctgaatgc gctgatcatg tcattggtcg cggcgcagta aggccgggga
     3541 taccgggaca gttcggcaaa acggcaggtg ccggcaagat actcagatgc cggagaaaac
     3601 cattggaatc gtactggtac ggaagcgggc ttcggcccgc ttttttttgg ctggccagcg
     3661 atactgtttt gacatttgtt atatttttct gttgtaatat ttttttaatt tggtgaaaaa
     3721 acatatatcg taaaggagct gaaataagta taatattcat gttaaattca gcttaaccag
     3781 tttaccgaca tccggaggat ttttatgaga ctgaggaatg tgcttgcggc aatggcagtt
     3841 gccgtctctc ttctgcttgc ggcctgcgtc ggcagtacgc cgatcgagaa gtacatgaac
     3901 gaggacatcg caggcatagg cgtggagaag aacatggaag aggcaggcac gatgctggag
     3961 gagatcctgg gcgaatgcgc tgagaagttg gaaaacggag aggaagtaga ctgggacgaa
     4021 gtcgagcaga aagtcgatcc tgtgcttgcg ggcatgcagg aaaccaggga caaagttgcg
     4081 cagctcgaga taaccgatga agacgtgaag gagatcaact cctacctggt ttccacgtat
     4141 gacaagctca tcagcggcta tgaaaagatg atcgccggct tcagagacat ggatgacgaa
     4201 ctgatcaacg aaggctcgga cgatctcacc ggagcccagg aagacatatt gaaatggtat
     4261 gaactgatag aagaggcaat caagtaatga gttgactaaa tctcatcagc atcttataca
     4321 gcccctattg gaaaaggctg gcgcacgata aacggcgccg gtcttttgtt atgtttattt
     4381 gatctttgcc ttgtttagag ctttaatttt gccatacatt cttcgattat tttctatttt
     4441 tatttgtcga gtgccattag gttacagaag gaaatttgct cctttttgct atatctatgg
     4501 cgtttaagga ggtcataata attacctgtc tcttactcga tttttataga attttggcag
     4561 attgtctgga aaaatacacc tgaaagaatt attatatata tagaactgaa gtcatttatc
     4621 ctgcttaaat tgtgctgata ccgttacatg taataaacag tggtatctaa gaatatttta
     4681 tatacttttc acttaagtga aaatacttag gaaactacag gacgatgata gcttttgttg
     4741 atactgaaat aggatttctt aaaaaagatg tactggatat cgggtgcgtc agggatgacg
     4801 gtgggcagtt ccgttccggt gtaatttctg cgttcattga tttccttaaa ggttcagaat
     4861 atgtatgtgg acataatatc ataaaccatg acatgaaata tatcggagat gcagtcaggg
     4921 cagctggcat tacagaggtc atagatactc tgtacctctc accgctgatg tttccggcca
     4981 gaccttatca caagcttctg aaggatgaca agcttcagac cgacgacatt aataatcccc
     5041 tcaatgattc aatgaaagca agggacctgt tctatgatga agttgaagca ttcaggcaga
     5101 ctgatgatac gttgaaaagg atttattact tgttgctgaa agatcagaag gagttcaggt
     5161 cgtttttcag atatctgggg tatacggaac acggagacgg taatcgggag gacagggatt
     5221 gcgaagccgg cagggaagca gttcagtatg aggaaatttc cgagcagcag gcagaagaac
     5281 ttgaaacgct catacgcacg agattcatac tcaggatatg cgaaaatgct gatctgtccg
     5341 gtatgatcat gaacagcccg gttgagcttg catattgtct ttctttgatt aacaccggtg
     5401 accgttattc cattacaccg ccctgggtcc tcaaaaatta ccctgctgtt gaccgcctgt
     5461 tatatttgtt gagagacaag ccatgtgtca cgggctgcgc ctattgcaat gaagcgctgg
     5521 atatttacag cggcctgcag aatttctttg ggtttgaatc gttcaggaca tatgccggta
     5581 tcccactcca ggagcaggca gtcagggctg ccattgatca gaaatccttc ctggcgatat
     5641 tccctactgg gggaggcaaa tctgtcacat tccagcttcc cgcgctgatg agcggcatta
     5701 acaccagggg cctgacagtc gttatttcac cgctgcagtc cctcatgaag gaccaggtgg
     5761 acaacctcga gaagttgaat attactgagg cagtcacgat caacggactg ctgaatccca
     5821 tagagcgtgc gaaggccatc gaaagagtcg aaaacggatc ggcatgtata ctatacattg
     5881 cccctgaatc gctgcgatca aagaccgtgg aaaacttgct gctgggcagg aaggtcgtcc
     5941 gtttcgtcat cgacgaagcc cattgcttct cggcgtgggg ccatgatttc agggtggatt
     6001 acctttacat cggtgatttt ataaggaacc tgcaggaaaa gaagagactg gacgaaccca
     6061 tccccgtctc ctgctttacg gccactgcga aacaaaaggt catcgaggac ataagggagt
     6121 atttcaggga aaagctatcc attgagatgg aactttttac ttccgatgct gtcaggacca
     6181 acctgcatta taaggtgttg cccagggaaa cggacgatga gaagtacaac accatcaggg
     6241 atttgttgag cgagaacgac tgtccaacta tcatctatgt ttcaagaacc aggagagcat
     6301 atgacctggc acaaaggctg acagcggacg ggtttgaggc aagagcctat cacggacaga
     6361 tggacgtcaa tgaaaaaaca gagaaccaga atgcgttcat gaacggtaaa gtgcggataa
     6421 tggtagcgac gtcggccttc ggcatgggag ttgacaaaaa ggacgtgggg atggtcatac
     6481 attatgagat atcggactcc cttgaaaact atatccagga agcaggaagg gcaggccgcg
     6541 atgaatcggt cgatgctgac tgctatgtgc tgttcaacga cgaagacctg aacaagcatt
     6601 tcatattgct caaccagacg aagatcagca taaaggagat ccagcaaata tggcgggcta
     6661 ttaagaacat tacgaaacag cgcatgagag tttcgcgctc ggcgcttgaa atagcgcggc
     6721 aggcaggttg ggatgacagc ataagcaata ttgaaacgaa ggtgaggaca gctatagcag
     6781 cgctggaaga ggcaggctac ctgaaaaggg agcaaaatgt gccccgcgtg tacgccacca
     6841 gcatcctggc tccaaatgcc cgcgaggcga tcgaaaaaat caatgcatcg cccaggtttg
     6901 acgaaaacca gaaagtaaag gcagcaagaa tcatcaaaaa cctcatttcg gcaaaacatt
     6961 ggaagacgcc agagctcgga gaagaacctg aatccagggt ggattacata tcagacatcc
     7021 ttggcatacc aaaagaagaa gtaatagaga tcatatacct gctgcgggat gaaaaaatac
     7081 tcgcaaatac aaaagatatg accgcgtaca tcaaaaagaa aagacagggg aatggttcgc
     7141 tggatatatt gaaatcgcat gtccagatcg aaaattttct gctcggcata tacaaaaacc
     7201 gggccgaagc gtacaatata aaaaagctca acgaacaggc ggttttgagc gggtgtgccg
     7261 aagcaactgt atccaggctg aggaccatca ttaacatctg ggcgattacc aagtggataa
     7321 agagacatta tgacaccagg tcgaatgtgc atttgaagct gcggggtctg atcagctgcg
     7381 aggaccttcg ggcaaagctt gagaaacggc atgaactgtc ccagttcatt attgagtacc
     7441 tgtacaggaa atgcggcgac gaaaaggatg tcggggaagg aacaaagagc ggggaagaga
     7501 taccggtgga gttctctgtg gtggaactta agcaggagtt tgaaagtgag attagacttt
     7561 tcagcactga agtgtcactt gccgaggtgg aggatgcact cttttacctg tcaaggatcg
     7621 gggccctgga actggaaggc ggttttctcg taaaatacaa cgcgatgatg atcgagcgtc
     7681 tagaaaggga caacagaagg cagtataggc ttgaggatta ccaggagctt gaacagtttt
     7741 accagaacaa gatacaacag atccatatag tgggcgaata tgccaggaaa atgatggagg
     7801 attaccaggc cgccctgcag ttcgtggacg attatttcaa gctgaattat tcatcgttcc
     7861 tcaacaagta cttcaagggc agcagacagg acgagatcag aaggagcata acaccggaaa
     7921 agttcaaaaa gctgttcgga gagctttcgc ctacacaact gaaaattatc aacgacaagg
     7981 attcgaagca tatcgtcgtt gctgccggac ccggaagcgg gaagaccagg atacttgtcc
     8041 acaagctggc ttctctcctg ctgatggagg acgtaaagca cgagcagctt ctgatgctga
     8101 cgttttcaag gtcggcggcc acagtcttta agaagcgcct gctgaaactg tacgggaacg
     8161 ccgctaatta cgtggagatc aagacattcc actcatattg cttcgacctg ctggggaagc
     8221 caggtacact ggaggcctcg gacaatatca tacgtgaggc ggtggaaaag atccgccggg
     8281 gtgaaacagg acccggaagg ataaccaaaa cggtgctggt tatagatgaa gcccaggaca
     8341 tggacgagga cgagtatgaa cttgtttgtg cgcttatgga acagaacgaa gagatgcgcg
     8401 ttatagctgt gggcgacgac gaccagaaca tttttgcttt caggggttcc agttctgaat
     8461 acatgaagaa gctcataacc gagaaaaacg ccgtagtata tgaattggcg gagaactaca
     8521 ggagcaaggc caatatcgtc aagttcacga acgagatcgc taaaaagata ccacacaggc
     8581 tgaaacggac acctattata cctgtgcaga aggacaatgg cgtaatcaag atcgtaaggt
     8641 ataagtccaa taacctgata acgccccttg tggatgatat tatgtcaacc gacctttcgg
     8701 gaacgacctg cgtcctgacc gccacgaacg acgaagcggt gaaggttgcc gggctgctgg
     8761 tgaacagggg catgccggcg aagctgatcc agtcggagga gggctttaag ctgtatgacc
     8821 tgcaggaagt gcggtacttc atgggcctgc ttgatgtgga taatccatca cctgtcattg
     8881 gcgctgatga ctgggaggag gccaagcgga agctgaaggg caggttcggc ggaaaccgcc
     8941 aaagcggcag cttgttgtcc ggcctgactg ccaacccgaa ccttgaactg tgcctgaaca
     9001 tgataaaaaa cttcgaagaa gcaaacccaa agctgaaatt caggtccgac ctggaaacgc
     9061 tcatcaggga gtccagactt gaggatttct atgaagagcg gggagaaacc atccttgttt
     9121 ccaccatgca taaggcaaaa ggcagggaat tt
//
LOCUS       DAOSCC010000090         9085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5007_length_9085_cov_4.393245, whole genome shotgun sequence.
ACCESSION   DAOSCC010000090 DAOSCC010000000
VERSION     DAOSCC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5007_length_9085_cov_4.393245"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLG72_06195"
     CDS             <1..469
                     /locus_tag="PLG72_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOJ80422.1"
                     /translation="VRGSAGDITGYAMRGGKIFVKGNTGYRTGIHMKEYRDRKPVIVV
                     GGTVGSFLGEYIAGGIIIVLGLGTDEFPAGNFTGTGMHGGQIFIRSLKKPKYLPKQVC
                     VSLADISDISSIKEYLSEFSELFGIGMDVILEKPFYKLTPNAKNPYKQMYTYN"
     gene            549..1637
                     /locus_tag="PLG72_06200"
     CDS             549..1637
                     /locus_tag="PLG72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOJ80423.1"
                     /translation="MLNKAYLVLETGKTFEGYSFGAEREVIGEVVFTTCMTGYLETLT
                     DKSYYGQIIMHTYPLIGNYGVIPEDFESNAVSALGYIAKDVCTDPSNFRSKGDLGTFL
                     KERDIPGLCGIDTRELTKTLREQGTMNGIITRDPKKADLDKIRAYSVKNAVESVSVKE
                     RKTVNNGGKYTVVLLDFGYKESIVKELAKRNCRVVIMPHDASVQDILEQNPDGIMLSN
                     GPGDPADNPGIINNLKQLIAFRVPIFGICLGHQLLALARGFETRKLKYGHRGSNHPVK
                     DLDTGQLYITSQNHGYYVVNESIDSSIARMSFINLNDRTCEGIKYLDSPAFSVQFHPE
                     GSGGPKDTVHLFDEFLKEAGAYAAGREN"
     gene            1615..2466
                     /gene="dapF"
                     /locus_tag="PLG72_06205"
     CDS             1615..2466
                     /gene="dapF"
                     /locus_tag="PLG72_06205"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ80424.1"
                     /translation="MRQGGKIEFTKMHGCGNDYIYINCFEKEIDDPEELAIALSDRHF
                     GIGGDGLVLICRSDIADAKMRMFNMDGSEGKMCGNAIRCVAKYLYDNGLCKKDEIKIE
                     TLSGIKTLHLNIENCSVSSVKVDMGKAEFDPLKIPVALPGDRIINEPLNIRGQTYNIT
                     CVSMGNPHAVVFCDDTDGLDIAGLGPCFEYNGIFPERVNTEFVQVIGRNTIKMRVWER
                     GSGETLACGTGACAAAVASVLNGHCDKGQDIRVLLRGGELVINYTDERVLMTGNCVKV
                     FEGVIEF"
     gene            2597..4207
                     /locus_tag="PLG72_06210"
     CDS             2597..4207
                     /locus_tag="PLG72_06210"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ80425.1"
                     /translation="MTKFIFITGGVVSGLGKGITAASLGRLLKQRGLKVAAQKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDEDLTRFSNLTSGRVYWNVLNRE
                     RAGGYLGKTVQVIPHITGEIIDFIYNGAEKSGCDVLITEIGGTTGDIESQPFLEAIRQ
                     ISLDKGSDNCLFIHVTLVPYLKGSNEHKSKPTQHSVKELRSMGISPDIIIARTDEPFD
                     DDIKAKISLFCNVKLDCVIQNLTLPNLYEAPLMLHRNGLDDVVCRELDLHTKEPDLAE
                     WEDMVDKIKNRKKEVVIAIVGKYVKFPDAYLSIIESLNHAGYHTGANVRIKWVESETV
                     TKENVAEKLRGASGIIVPGGFDARGIEGKIVACQFARENDIPYLGICLGMQIAVIEYA
                     RNVCGLADANSTEFRESCLNPVINLMEDQLGITRKGGTMRLGAYPCVTREGTMFRSLY
                     GKELISERHRHRYEFNNAYREIMTGHGMVICGTAPDDSLVEAIELPDKKFFIGVQYHP
                     EFKSRPNKPHPLFMGLVKAALDKDTQAD"
     gene            4288..5466
                     /locus_tag="PLG72_06215"
     CDS             4288..5466
                     /locus_tag="PLG72_06215"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ80426.1"
                     /translation="MKINRNYLDLKESYLFAVIADKVDDYRKQNPQKEIIRLGIGDVT
                     LPLVPAVIEAMHKAVSEMGNADTFKGYGEYQGYDFLRQAVCDYYARKDISLDMGEVFI
                     SDGAKSDIGNILDIFDTDNTVIIPDPVYPVYVDTNIMAGRKIIFIDGNEKNGFLPLPD
                     EKTEGDIVYICSPNNPTGAVYTKDQLRMWVDWALERQAVILFDSAYEVFIRDPDLPTS
                     IYQVPGARKCAIEFCSLSKTAGFTGVRCGYTVIPDKLVRDGIPLNKLWYRRQSTKFNG
                     VPYIVQRGAEAVFTDTGLKQISEQIGYYLENARIMADALAGLSVWFTGGKNSPYIWLK
                     CPNGMSSWEFFDFLLNNCGVVGTPGEGFGKNGEGFFRLTAFGNRGNVTEAMERIRKAL
                     "
     gene            complement(5625..5939)
                     /locus_tag="PLG72_06220"
     CDS             complement(5625..5939)
                     /locus_tag="PLG72_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HOJ80427.1"
                     /translation="MRYTYKTKNTCSRMISFDIDENDVVTNIEFTGGCSGNLKAIPKL
                     VDGWTAQQIAGKLLGNTCGDRRTSCADQLAIAVLEASRELKKQKNTSTATSLQSTMST
                     GI"
     gene            6215..6712
                     /locus_tag="PLG72_06225"
     CDS             6215..6712
                     /locus_tag="PLG72_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ80428.1"
                     /translation="MAEFGEQLRKAREVKGMTQQSLAEKLYVTRQAVSRWECGDRYPD
                     LITAKRIAQILDVSLDDLISEKDMKQVAERNPIVEKPSINNIVIAQFAFVLFACIVST
                     VNMFIRNPLDLLPASYDDLLIIGTNLLSKIIQAGIFAFGLIMAVKGMLSPKMTVECRS
                     DLDIC"
     gene            6702..7508
                     /locus_tag="PLG72_06230"
     CDS             6702..7508
                     /locus_tag="PLG72_06230"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ80429.1"
                     /translation="MYVKAASYIWFLGIMALLVYSLVSTLLLKRQLKNARSTEENIFE
                     ANNLETPFLLGIIKPKIYLPAGLDDAERSYILLHEQTHIHRKDHIIKAFAFLTLSVHW
                     FNPLAWIAFRLMTADMELSCDEKVLKEMDGDIRKPYAATLLSLAAGRHILSGNPLAFG
                     EGDVKQRIRNVLNYREPAFWLTLTAVIAAVAVGIGLLANPEGTNIIEVEEDPDAPVKY
                     IQIEITQPGSRELTGRVITDKDGYKAGDTVSVVISESVTYDVESLVKGAI"
     gene            complement(7456..8049)
                     /locus_tag="PLG72_06235"
     CDS             complement(7456..8049)
                     /locus_tag="PLG72_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ80430.1"
                     /translation="MYHTDFAVNVNNVTKTFIQWQRDNTGKGLIRNLIKPEKRKITAL
                     DNVSFTIGKGEFVAYAGPNGAGKSTTMKLLTGMLLPDSGDISVLSLSPQKQRAKLMEH
                     LGILFGNRTELWWDHPVIQSFEWKKVVWGILDDVYRKNLDMLIELLDIGKLLKTFARE
                     LSLGQRMRADLAMMLLHSPEVILRPLQGSQHHKLRFR"
     gene            complement(8714..8956)
                     /locus_tag="PLG72_06240"
     CDS             complement(8714..8956)
                     /locus_tag="PLG72_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80431.1"
                     /translation="MNIAAFCSNGKACVKCEHIKGIAYKYAALGVESALLPVICAILA
                     LLREYCALFSVSDTSRRKHMPPGKANSDACFAASAA"
BASE COUNT         2467 a         2076 c         2402 g         2140 t
ORIGIN      
        1 cgtccggggg tcggcggggg atatcaccgg ctacgcgatg cgcggcggga aaatattcgt
       61 gaagggcaat accgggtacc gtacgggcat ccacatgaaa gaatacaggg acaggaaacc
      121 tgtcatagtg gtaggaggca ctgtcggaag tttccttgga gaatacattg ccggcgggat
      181 tataatcgtc ctgggcctgg gcactgacga atttcctgcg gggaacttca caggcacagg
      241 catgcacgga ggccagatat tcatcagaag cctgaaaaag ccaaagtatc tgccaaaaca
      301 ggtatgtgtg agcctggctg atatttcgga tatcagttcc ataaaggagt acctgtccga
      361 attctcggaa ctgttcggca tcggtatgga tgtgatactg gagaagccgt tttacaagct
      421 tacgccgaac gcaaagaacc cgtacaagca gatgtacaca tataactaac gggtgcgatt
      481 gccggataaa tcctgctttt acagattttt accatattgc aaacttctta tagacaggtg
      541 agaggaatat gctaaataaa gcgtacctgg ttcttgagac agggaaaaca ttcgagggat
      601 attcattcgg agcggaacgg gaagtcatag gggaagtcgt ttttaccacc tgcatgaccg
      661 gatacctgga aacgctgact gataaaagct attacgggca aataataatg catacctatc
      721 ccctgatagg caattacgga gttattccgg aggactttga aagcaatgcg gtttcagcgc
      781 ttggctacat tgcgaaagac gtgtgtaccg atccatcaaa tttcagaagc aaaggcgatc
      841 tcggcacttt cctgaaagag cgcgatattc ccgggctatg cgggatcgat accagggaac
      901 tgacgaagac actgcgcgaa cagggcacga tgaacgggat aatcaccagg gatcccaaaa
      961 aggccgatct ggacaagatc agggcttatt cggtaaaaaa tgcagttgaa agcgtttcgg
     1021 taaaggaaag aaaaaccgtg aacaacggcg ggaaatatac agtcgtgctc ctcgatttcg
     1081 gctataagga aagtattgtt aaagaactgg caaaaaggaa ctgcagggtg gtaataatgc
     1141 cgcatgatgc atctgtgcag gacatacttg agcagaatcc ggacggtata atgctgtcca
     1201 acgggccggg cgaccctgca gacaaccccg gtatcataaa caacctgaaa cagcttattg
     1261 ctttcagggt cccgatcttc ggcatatgcc tcggccatca gctcctggcg ctggcccgcg
     1321 ggtttgagac aaggaagctg aaatacgggc acaggggttc caatcatccc gtaaaggatc
     1381 tggatacagg ccagttgtac ataacaagcc agaaccacgg gtattatgtc gttaatgaaa
     1441 gcatcgacag ttcgatcgcg cggatgtcgt tcataaacct gaacgacagg acctgtgaag
     1501 ggataaaata cctggactcg cccgcatttt cggtgcaatt ccatccggaa ggcagcggcg
     1561 gccctaaaga cacggtccat ctgttcgatg agtttttgaa ggaggcaggc gcctatgcgg
     1621 cagggcggga aaattgaatt caccaagatg cacgggtgcg ggaatgacta tatatacatc
     1681 aactgtttcg aaaaagagat cgacgatccc gaggagcttg ccatcgcctt gtccgaccgg
     1741 catttcggca taggcggcga cggcctggtc ctgatatgca ggagcgatat agccgacgct
     1801 aaaatgagga tgttcaacat ggacggaagc gaaggcaaaa tgtgcggcaa tgctatacgc
     1861 tgcgttgcca aatacctgta tgacaacggc ctttgcaaaa aggatgagat aaagatagag
     1921 actttgagcg gtataaaaac actgcatctg aacattgaaa actgttcggt atcatccgtg
     1981 aaggtagata tggggaaagc tgagtttgac cctttgaaaa tacccgtcgc attgcccggg
     2041 gaccgcatta taaatgagcc tttgaacata aggggccaaa cttacaatat cacctgcgtg
     2101 tccatgggaa atccgcatgc cgtggtcttc tgcgatgata cggacggcct ggacatcgcc
     2161 ggattaggac cgtgcttcga gtacaacggg atattccctg aacgcgtcaa taccgaattc
     2221 gtgcaggtga tcggcagaaa cacgataaaa atgcgcgtat gggaacgggg aagcggggaa
     2281 acacttgctt gtgggacagg cgcctgcgcc gcggccgtcg cgtcggtttt gaacggacat
     2341 tgcgataaag ggcaggatat ccgggtgctg cttaggggcg gcgaactggt gatcaattat
     2401 accgatgaaa gggtgctcat gacaggaaac tgtgtcaagg tattcgaagg cgttatcgaa
     2461 ttctgagcat gtttgcagcg gaatgcgggc agctttgcct gaaatggcag catgagttca
     2521 ggtattgcat tataaggcag gaattgcgaa acgaagtaaa aggtaaacat atgaaaaaga
     2581 aaagggatga atcgctatga caaaattcat tttcataaca ggcggagtcg tatcaggact
     2641 tggaaaaggc attaccgccg caagcctggg caggctgctg aagcagagag gcctgaaggt
     2701 cgccgcacag aaactggatc cgtatataaa cgtggacccg gggacgatga gccctttcca
     2761 gcacggagag gtgttcgtta ctgacgacgg ggcggagacc gatctcgacc tgggacatta
     2821 tgagcgtttc atagacgaag acctgaccag gttttccaac ctgacttcgg gccgggtcta
     2881 ctggaatgtg ctcaaccgcg aacgcgccgg aggctacctg ggcaaaacgg tccaggtcat
     2941 ccctcacatt accggcgaga taatcgattt tatatacaac ggtgctgaaa aaagcggctg
     3001 cgatgtgctg ataactgaaa tcggaggaac gacgggagac atcgaaagcc agcctttcct
     3061 tgaggccata aggcagatct cccttgacaa gggcagcgat aattgccttt tcatccatgt
     3121 gacgctggtc ccgtacctga aaggatccaa tgagcataaa tctaagccta cgcagcattc
     3181 ggtgaaggaa ctcaggtcga tgggcatttc gccggacatc atcattgccc gtaccgatga
     3241 gcctttcgat gatgacataa aggcaaaaat atcgctgttc tgcaatgtca aactcgattg
     3301 cgtgatccag aacctcactt tgccgaacct gtatgaagcg ccgctcatgc tgcaccgaaa
     3361 cggccttgac gacgtggtat gcagggaact ggacctgcat acgaaagagc cggacctggc
     3421 cgagtgggaa gacatggtcg ataagataaa aaaccgtaaa aaagaagtgg tcatagccat
     3481 cgtaggcaaa tacgtgaagt tccctgatgc atatctttcg ataatagaaa gcctgaacca
     3541 cgcaggttac cacacaggcg cgaatgtaag gatcaaatgg gtggagagcg agaccgtcac
     3601 gaaagaaaat gtggctgaaa aactgagagg agcaagcggg atcatcgttc ccggcgggtt
     3661 cgacgccagg ggcatcgagg ggaagatcgt cgcctgccag tttgcacggg agaatgatat
     3721 cccttacctc gggatctgcc tcggcatgca gatagccgtc attgaatacg cacgcaatgt
     3781 ctgcggcctt gcggatgcca attcgacaga gttcagggaa agctgcctca accccgtgat
     3841 caacctgatg gaagaccagc tcgggatcac aaggaaaggc gggaccatga ggctgggcgc
     3901 atatccgtgc gtaacgcggg aaggcaccat gttccggtct ctgtacggca aagaactgat
     3961 aagcgagcgc caccgccaca ggtatgaatt caacaacgcg taccgggaga tcatgaccgg
     4021 gcacgggatg gtcatttgcg gtacggcacc cgacgacagc ctggtcgagg cgatagaact
     4081 gccggataag aagttcttca taggggtaca gtaccaccct gaattcaaga gccggcccaa
     4141 taagccgcat ccgttgttca tggggcttgt gaaggccgct ttggataaag atacacaagc
     4201 ggattagcgc tgtttgatac ggacaaatct tgacttactg tatttttgat ctatctggac
     4261 cagttttata ttgtgaggtt ggcagttatg aaaatcaaca ggaattatct tgatctgaaa
     4321 gaaagttatc tgtttgcagt tattgcggac aaggtcgatg attacaggaa acaaaaccct
     4381 cagaaggaaa ttatcaggct tgggatagga gatgtcacac tgccccttgt tccggcagta
     4441 atcgaggcaa tgcacaaagc tgtttcagaa atggggaatg cggacacctt caaaggttac
     4501 ggggaatacc aggggtatga tttcctgagg caggccgtat gcgattatta cgcgcgcaag
     4561 gacatatcgc tcgatatggg cgaggtgttc atcagcgacg gggctaaaag cgatatcgga
     4621 aacatcctgg atatattcga taccgacaac acggtgataa tccccgaccc ggtctatccc
     4681 gtgtatgtgg acacgaatat catggccggc cggaaaatca tatttataga cggcaacgag
     4741 aagaacggat ttctgccatt gcctgatgaa aaaacagaag gggacatcgt ttacatctgt
     4801 tcgcccaaca atcccaccgg cgcggtctac acgaaagacc agctgaggat gtgggtcgac
     4861 tgggcactgg aaaggcaggc agtcatcctg ttcgacagcg cctatgaagt gttcatccgt
     4921 gatccggacc tgccgacaag catttaccag gtaccgggcg caaggaaatg cgccatcgag
     4981 ttctgctccc tttccaaaac ggcaggcttc acaggagtac gctgcggcta tacggtcatt
     5041 cccgacaagc tggtgaggga cggtatacct ttgaacaaac tgtggtacag gcgccagtcg
     5101 accaagttca acggggtgcc ttacatagtg cagaggggcg ccgaagcagt gttcacggat
     5161 accgggctga agcagatatc ggagcagatc ggttattacc tcgagaatgc acgtattatg
     5221 gcggatgccc tggccgggct cagcgtctgg ttcacgggag gcaaaaactc gccgtatatc
     5281 tggctgaaat gcccgaatgg catgtcgtca tgggaattct tcgatttcct cctgaataac
     5341 tgcggcgttg tcggaacacc gggagaaggc ttcgggaaaa acggagaggg cttcttccgc
     5401 ctcactgcct tcggtaacag gggaaatgtt acagaagcga tggagaggat aaggaaagca
     5461 ttgtaatatt gcattctccc tgagattcgt tctcctgtat ataagttgtg ttttccgtat
     5521 atgtatgata agtatgttga tatacggaaa acgcagcttt tttattgcca gagagcccaa
     5581 tgtatatatt gctgcttata taatcctgaa tgttaaatac ttatttaaat gcccgttgac
     5641 attgtggact gcagggaagt tgcggttgat gtattttttt gttttttgag ttccctggag
     5701 gcttccagaa ctgcaatcgc cagctggtcc gcacatgagg tccgtcgatc gccgcaggta
     5761 ttgccgagca gcttcccagc gatctgctgt gcagtccagc catcgaccag tttgggaata
     5821 gccttgagat ttccgctgca gccgcctgta aactcgatat tcgtgacgac atcattctca
     5881 tcgatgtcga aagagatcat cctggagcac gtgttttttg ttttgtatgt atatctcatt
     5941 attctgcctc cgataagttt tacagcactt ttgccggtat ctcccggatc ttgcctgaag
     6001 tgttggtatc ccgggataat gcaactttac ccacctttaa aatgtacaca ataatattta
     6061 tccttgcaag tcgttttctc tgtcaccaaa acggtgatac tacgttttta cagtgttgct
     6121 tgcttttatg ctgctgacgg atgataataa aaccacactc gcacatgcat ataatcatca
     6181 gtgtgggtaa caagaagacg aaaggagctt ttttatggct gagttcggag aacagctgag
     6241 aaaggcaaga gaggtcaagg gaatgaccca gcagtctctt gcggaaaaac tgtatgttac
     6301 acggcaggca gtatcgagat gggagtgcgg cgaccgctac cccgatctga ttacagccaa
     6361 aaggatagct cagatcctgg atgtgagtct ggacgatctg atttcggaaa aagatatgaa
     6421 gcaagttgca gaacggaatc cgatcgttga aaaaccttcc atcaacaata ttgtgattgc
     6481 acagttcgca tttgtgcttt ttgcctgcat tgtctcgaca gtgaatatgt tcatcaggaa
     6541 ccctttagat ctgttgccgg cttcatatga tgatttgctg ataatcggga caaatctttt
     6601 gagtaaaatc atccaggcag gtatcttcgc tttcggactg atcatggctg taaaagggat
     6661 gctttctccg aagatgacag tcgaatgcag atccgatttg gatatatgtt aaggcagcat
     6721 cttatatatg gtttttgggc ataatggcat tgcttgttta cagccttgta tccactttac
     6781 tgctgaaaag gcaattgaaa aatgcaagat caacagagga aaacatcttc gaagccaaca
     6841 acctggaaac accgtttctg cttggaataa taaaaccgaa gatatacttg ccggctggac
     6901 ttgacgacgc tgaaagaagt tacattctgc tgcatgagca gacccatatc caccggaaag
     6961 atcatataat caaggcattt gcatttctga cattatccgt ccactggttc aatcctctgg
     7021 cctggatcgc gttcaggctg atgaccgcgg atatggaact gtcctgtgat gaaaaagtcc
     7081 tgaaggaaat ggatggagat ataaggaagc cttatgccgc tacattgcta tctcttgctg
     7141 caggcaggca tattttgagc ggaaacccgc ttgctttcgg cgagggggat gtaaaacaaa
     7201 ggataaggaa tgtcctgaac tacagggaac cggccttctg gctgacattg actgctgtaa
     7261 tagcagcagt tgcagtgggg ataggtttgc tggctaatcc tgaaggtaca aacattattg
     7321 aggttgaaga agatcctgat gctcccgtga aatatataca aatagaaatc acccagcctg
     7381 gcagccgtga actgacaggc agggttataa ctgacaagga cggctataaa gccggcgata
     7441 ccgtatctgt tgtaatcagc gaaagcgtaa cttatgatgt tgagagcctt gtaaagggcg
     7501 caatatgact tccgggctgt gcagcagcat catggcaagg tcggccctca tgcgctgtcc
     7561 gaggctgagc tcgcgggcga acgttttcag cagtttccct atatccagaa gttcgatcag
     7621 catgtcgaga tttttcctgt aaacatcatc caggatcccc cagacgacct ttttccactc
     7681 gaagctttgg ataacagggt ggtcccacca cagttccgtg cggttgccga acaggatccc
     7741 aagatgctcc atcagctttg ccctctgttt ctgcggagac agggacaaaa ccgatatatc
     7801 gccgctgtcc gggagcaaca tgcctgtaag cagtttcatc gttgtgcttt tgcctgcgcc
     7861 gttgggtccg gcgtaagcta cgaactcacc cttcccgatc gtgaatgaaa cgttgtccag
     7921 ggctgtgatc ttccgctttt cgggctttat gaggttcctt atcaggcctt tgcccgtatt
     7981 gtcacgctgc cactgtataa aggtctttgt cacgttattt acgttaacgg cgaaatccgt
     8041 atggtacata cctgtttgag ccttttgtaa cataccagtt cattcctttc ctgaagaaat
     8101 atttagctgc aagtgccaga acaagcgcat atgcaaacgg ataatattca gtgaggttca
     8161 aaggcggctt tcccagcagg cataatgcag ggaaccatgc catgagccct tccggcagga
     8221 tggtgagcag ggggacctgt atgaaggcag gcatccccga cagcggaaac ggttgcctga
     8281 gtcctgacga ccatcatatc ggaaagcagt gcacctgcat atgtataggt cagcatctgg
     8341 tccatggtca tacctacgtc tgcgccggat gacatgactg ccttccacat atatatcaat
     8401 ggtatgagag tacagatctt tatgaatata tccggcataa gatacagtac gccgccgttt
     8461 gttttttcca agcacgccat ttgagccgat tttatatacc tgatcatatt ttttctactc
     8521 cttaagcagt cgttccggtt tccggccgga tggcaccgct ttaaaactta gtcgggcccg
     8581 gtcggatgat ttccggtcgg ctgattattg tcagatagca tatttgcgca acaaaaaacc
     8641 caggtatccg atcgaatacc tgggcaaact ttcttatttc aaactttccg gccagaagcg
     8701 acggcctgcg cggtcaggca gcgcttgctg caaagcaggc atcactattc gccttgcccg
     8761 gcggcatatg tttgcggcga gaggtatccg acacggaaaa aagggcacaa tactcccgta
     8821 acagggccaa aatggcgcaa ataacgggca aaagggcaga ctccacccct aacgccgcat
     8881 acttgtatgc gatacctttg atgtgttcgc atttcacaca cgccttgccg ttactgcaaa
     8941 atgcggctat attcatacct ctcaccacct tccatcagaa tggatacatt atactatgac
     9001 aaatcgtgga tgtcaaatga gaagcatcct gtgatagcgt cagtttactg tagggaaaag
     9061 aagaaaagaa tcatcctgat gtttt
//
LOCUS       DAOSCC010000091         9057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5028_length_9057_cov_4.488447, whole genome shotgun sequence.
ACCESSION   DAOSCC010000091 DAOSCC010000000
VERSION     DAOSCC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_9057_cov_4.488447"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1143..1361)
                     /locus_tag="PLG72_06245"
     CDS             complement(1143..1361)
                     /locus_tag="PLG72_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80432.1"
                     /translation="MLPTFMQFAGNDVLVAHNAYNFDIKFLLAAACDIGIEIKNPIVD
                     TLPLCRKLYPIQLQILSKCSNRYKDFEI"
     gene            1490..2893
                     /locus_tag="PLG72_06250"
     CDS             1490..2893
                     /locus_tag="PLG72_06250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOJ80433.1"
                     /translation="MVFSSLVFLLRFLPATLLLYFTVPGMKAKNAVLIAASLFFYAWG
                     EPVWVILLIISSVIDYICGGFAGKYPNTPKGRLFMIISLVLNFGLLGFFKYGGFIVNN
                     INSLLGLNIPDPGVSLPIGISFYTMQTVSYTIDAYKGKAKVQKSYARFLLFVSMFPQL
                     VAGPIVRYTDVEDQLEERTVTMQGIYEGITRFVVGLAKKTLIANFAGSVAAKYLDGDL
                     LGMSAPEVWFGLLCFTFQIYFDFSGYSDMAIGLGKVFGFTFPENFNYPYTAQSITDFW
                     RRWHMTLGTFFRDYVYIPLGGNRRLQMRNILIVWMLTGLWHGASWNFVIWGLYFAVIL
                     VLEKKFLLKALEKVPEAVRTLYSFLLVMFGFNFFYFEDFSRNIDALKVMFGLAGASLP
                     AADVFRLVVSYLPFIAVCVICSLPVKRFILDRAEKLRLPEGVRAAAAALCIAIVFIMS
                     AASIVSNSYNPFIYFRF"
     gene            2938..4560
                     /locus_tag="PLG72_06255"
     CDS             2938..4560
                     /locus_tag="PLG72_06255"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOJ80434.1"
                     /translation="MKKTQHAVICTFVALLALLIVCSLFVDSDGFSPFENRYLGRKPE
                     LTISGVFSGEFMDKYETFLLDNFLLRELSVTGFQAYADLLYLDLFTGSEIVLANVDVD
                     EFVKAGGQEGISAAGNGIAVEAGNEGTSGATGESAVSGGNADITAGQDSGNLQAPDGS
                     SAERYGLADRAQQSVNAGTQTVSAPGEATGAGQAAQNGEQDTEVQQEGQSAGDQTGRQ
                     DGGDNPGEGSAPVNEQADERLNNSLIIAGDRVMMPTGAYNLKAFGGILSEFGEIMPDL
                     NLYSITGPTSAAFYASKKYSTGNYDQSKAEAIIDQNADGVTVVRVFDRLMEHKDEHIY
                     FRSDVHWTALGAYYAYTAFCEASGQVAADINDDFTKGTYEPFLGGLYAQIYKMPQASR
                     LKNNPEKLDYYIPRIGHQLTLYKMGNLKDSYKVDSIINTNFKSLGAYKYSCFAWGDQR
                     IERIDTDNPNGRKLLVIKDSYGSALVPFLVHNYSLIYVIDPKGFNNEGCLDFDAKVLI
                     NDEGIDDVLFCFSIYGSGRKIIQDSLKSLLLR"
     gene            complement(4780..5292)
                     /locus_tag="PLG72_06260"
     CDS             complement(4780..5292)
                     /locus_tag="PLG72_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOJ80435.1"
                     /translation="MRKTALSERMYKLLADHITEIEREKEPILKSLFSDNAQTAMQSE
                     AFFREYINRLEKFLKNAEVKKDGADSCPFSIIGSVVQIKDLEDSEIINCRIVLPYAGK
                     SSAGLFDASCLSPMGQALLLNAPGSIVTVSTPAGQVKYEIVSISVDDLDIGESDLSYY
                     INKLKAVLAE"
     gene            complement(5585..>6790)
                     /locus_tag="PLG72_06265"
     CDS             complement(5585..>6790)
                     /locus_tag="PLG72_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80436.1"
                     /translation="FFRRRIRKVYRNVRTSLAKINSAISENISGMRVIQMFNREKENF
                     ERFGRIGHEYYRAVMSEIITFGLFRPAVEMMSYIALSLLIWFGGLEVLHGTLQFGVLY
                     AFVNYISMFFQPINDLAEKYNILQASMAAAERIFLILDTPAEDDSGALSLPDGVRGEI
                     EFRNVWFAYNEGDWVLRDVSFTVPAGKTVAIVGATGAGKTSIISLLSRLYEIQKGEIL
                     IDGINTRDIRKDDLRKLTGTVLQDVFLFSGKLKDNIRLDEESITDDKVREAARYVNAD
                     GFISKLPLGYDTEVMERGATFSAGQRQLLAFARALAFDPKILVLDEATSNIDTETEML
                     IQDALAKLTRNRTTIVIAHRLSTVQHADKIIVLHKGKVRESGTHQELLAKRGMYYSLY
                     LLQYQKQEP"
     assembly_gap    6791..6890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6891..7533)
                     /locus_tag="PLG72_06270"
     CDS             complement(<6891..7533)
                     /locus_tag="PLG72_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80437.1"
                     /translation="MADDNRENEGFAEEELQVKVYDFRLMKRLLGFAARYWYLFALAV
                     ILLFASTAADLARPYLIGVAIDDFIKKGDAASLSRMGVYFLLLVVSGFLFNLLQIYVL
                     SYAGQSIIFNIRQKLFSHMQKLPLSYFDKNPVGRLVTRVTNDTEALNEVYTNVLVTLL
                     KDIAILAGVVVIMFKLNVTMTLVSLAIMPVVIVLSVFFRRRIRKVYRNVRTSLA"
     gene            complement(7535..>9057)
                     /locus_tag="PLG72_06275"
     CDS             complement(7535..>9057)
                     /locus_tag="PLG72_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80438.1"
                     /translation="LQKLSANYFNTHKTGDIMAHVTNDMNNVTMATGQGVIFAVDCLL
                     MPVASLVMMLQTGGLRLTAASFAPILVLGILVFSYMRIMQSTIQRQQEAFSNLTEAAR
                     ENFSGIRVIKSFVQENHQTERFESVNEANRRANLRFIRIMSMMFPSVMLVSSLSFVIA
                     LWYGGLLVIDGAVSLGEFVAFNSYLGMLIWPITAVGWVANIFKRGSVSLDRINALMDE
                     KPEIADKTGAVTLVIPGAAALHVSEKADVSGKNPDGAVGTKAIEFRNLTFKYPGSREP
                     VLKDISLSVDHGKTLAIVGKTGSGKSTLINLVPRILSAPEGTLFINGIDINDISLSSL
                     RGNIGCVPQDTFLFSDTIKNNIDFYRGFSMEEIEKAAKMARLYDDIMEFPQKFDTLVG
                     ERGVTLSGGQKQRTAIARAIIGSPSILILDDCLSAVDTKTEEGILRELKAIMKNRTCI
                     IVSHRISAIRDADEIIVLDEGKIMERGTHDSLVEQGGYYHDLYTKQLLADEIEEAE"
BASE COUNT         2175 a         2137 c         2209 g         2436 t
ORIGIN      
        1 gtagctcagc aggtagagca gctgacttgt aatcagcagg tcgggggttc gagtccgtcc
       61 accagctcca agggtttcag gaattcttga agcccttttt tcatgtcaat gccgcacatt
      121 atgttgcaga tttttctttt tcatccatgt gttcctgttt gaggctgtaa aacttcttgt
      181 atggtaatga atgtgatgaa aaaatggaca gtaaaaggga gaaagtgacc aaaaaaacaa
      241 ccttccttgc cagacaagtt aaatttcaca taattatatt atgggtaagc gctgtccggt
      301 ccagaacatt attatggttg ttgcctgctg acacataaat aattcagccg ggaacatact
      361 gcctgatact gaaatatgaa tagctaaata attaagctaa atctcatgcc gaaaagatag
      421 taaccaccac agtacatgaa attctttaca ctaccttcat gcgtcaacag ttacacatct
      481 attatacaat aactgaaacg aactatatgc gtaagaagct gctttttcga caagccttgt
      541 acatttattt attatctgcc atagctgcga gttttcttgt ggcaatctag gcaaaggatt
      601 tcacagtttg acagtgtatc agccccgcca gaggaaacgg cagtcttatg gtgtgcttcc
      661 catgcacccc atgcgccttc ttttcccctg ttctcgaaag acaaagtctt attgcaacgc
      721 ccagtatgat tatgcgtaat cctagcgcac tcacactttc cgcctgatcg ttcccaagcg
      781 gcattgatag tttcctttga aaaggccatt atcttcacct ccatttttat actattggta
      841 gacattcaac ttgtaattat atgtgatcac tggtcttgta ttataggcat ctcttaacta
      901 ccattgtata ccaactataa tatacatcaa aaaaattaga aatcaatgtt aaatataaaa
      961 tcaaaataag gggaaatatc tcaaatatta tcaacggagg gaaatatcta ttactttata
     1021 ttagtaggct ggttttccct acttataaag agaatatggt atcttaaaat ggcggtgtca
     1081 ttataatata cttagaagtt aaggaccttg aatacttcat ttattgctaa ctgcactttt
     1141 tattatattt caaaatcttt atatctgttg gagcatttgc tcagtatttg caattgtatg
     1201 ggatataact tgcggcacag cggcaatgtg tcaacaatag ggttttttat ctcgatgccg
     1261 atgtcgcaag ctgctgcaag aaggaatttt atatcaaaat tataggcatt atgggcaact
     1321 aacacatcat tgcctgcaaa ctgcataaaa gtaggcagca cttctgagaa actgtccctt
     1381 tactttggac attcactgcc ttattgtaag gttggtaaat tgatggtatt atttacttgg
     1441 tattatttac atggttgctc aattatacat aagcaacttc ggaggacata tggtattctc
     1501 aagtttggta ttcctgctga ggtttttgcc ggcaacgctt ttgctttact tcacggtgcc
     1561 cgggatgaaa gcaaaaaatg ccgtactgat agcggcgtcg ctgttttttt atgcctgggg
     1621 agagcctgtg tgggtgatac tgctgatcat cagttctgtt atagattaca tatgcggcgg
     1681 atttgccggg aaatatccta atacgccgaa gggcaggctg ttcatgataa tttcgctggt
     1741 gctgaacttc ggactgctgg gttttttcaa atatggagga ttcatcgtaa acaacatcaa
     1801 ttcgctgctt ggcctcaata taccggatcc gggagtatct ctgccgatag gcatttcatt
     1861 ctataccatg cagacagttt catatactat cgatgcatac aagggcaagg caaaagtgca
     1921 gaagtcgtat gccaggttcc tgcttttcgt gtcgatgttc cctcagctgg tcgcaggccc
     1981 catcgtccgc tataccgatg tggaagacca gttggaggaa cgcaccgtca caatgcaggg
     2041 gatatatgaa ggaatcacac ggtttgttgt ggggcttgca aagaaaacac tgatagcgaa
     2101 tttcgctggt tctgtcgcgg ccaaatacct tgacggggac ctgctgggca tgtcggcccc
     2161 tgaagtgtgg ttcgggcttt tatgtttcac gttccagata tatttcgatt tttccggcta
     2221 ctcggacatg gcgataggcc tgggaaaggt tttcgggttc acgttcccgg aaaacttcaa
     2281 ttacccttac actgcacaga gcatcacgga tttctggcgg cgctggcaca tgacgctggg
     2341 aacgtttttc agggattatg tctacattcc gcttggaggc aatcgaaggc tgcagatgag
     2401 gaatattctt atcgtctgga tgctgaccgg cctctggcat ggagcaagct ggaatttcgt
     2461 catatggggc ctgtattttg cagtgatcct tgtgcttgaa aagaaattcc tgctcaaagc
     2521 gctcgaaaaa gtgcctgaag cggtcaggac cctatattcg ttcctgcttg tcatgtttgg
     2581 tttcaacttt ttctattttg aagacttcag caggaatatc gacgcgctga aggtgatgtt
     2641 cgggctggcc ggcgcatcat tacccgcggc cgatgtattc cggcttgtgg tcagctatct
     2701 tccgtttatc gcagtgtgtg tgatttgttc gctgccagta aaacgcttca tcctggacag
     2761 ggcggagaaa ctcaggcttc cggaaggcgt gagggcagct gcggcagcac tttgcattgc
     2821 catagtattt ataatgtcgg cagcaagtat agtgagcaac agctataatc cgttcatata
     2881 tttcaggttc tgaccggcgg caaaaaagca tcttaagcgg cggagaagag gaaagatatg
     2941 aaaaagactc aacacgcagt aatatgcaca tttgttgcat tattggcatt gcttatcgtt
     3001 tgctcgctgt ttgtcgacag cgacgggttt tctcctttcg agaacaggta tctcggcagg
     3061 aagcccgaat tgacgatatc aggcgtattc tcgggagagt tcatggacaa gtatgagact
     3121 ttccttctgg acaacttttt attaagggag ttgtcggtga ccggtttcca ggcttatgcg
     3181 gacctgcttt acctggacct tttcaccggc agcgagatcg tgcttgcgaa tgtggatgtg
     3241 gatgaattcg tgaaagccgg gggccaggaa ggcatttcag cggccggaaa cgggatcgct
     3301 gtggaggcag gaaacgaagg caccagtgga gcgacgggtg aaagcgcagt gtcgggcgga
     3361 aacgccgaca ttacggcggg gcaggattca ggcaacttgc aggctccgga cggcagttca
     3421 gcggaaagat acgggcttgc cgacagggcg cagcaatccg tcaatgccgg cacacagacg
     3481 gtatccgctc ccggagaagc aactggggca ggtcaggcag cgcaaaacgg ggagcaggat
     3541 actgaagtac agcaggaagg ccagtctgcc ggggaccaaa ccggccggca ggatggcggg
     3601 gataatcccg gagaaggaag tgcccctgta aatgaacagg cggacgaacg gctcaacaat
     3661 tcactgataa tagcaggcga cagggtcatg atgccgaccg gagcctacaa cctgaaggcg
     3721 tttggcggga tcctgtccga gttcggcgag atcatgccgg atctcaacct gtattcgata
     3781 accggcccga caagcgctgc tttctatgcg tcgaaaaaat acagcaccgg caattatgac
     3841 cagagcaagg ctgaagccat aattgaccag aatgctgatg gtgtcacggt tgtcagggta
     3901 tttgacaggc tgatggagca taaagatgag cacatctatt tccggtctga tgtacactgg
     3961 accgcactgg gggcgtacta tgcctacact gcgttctgcg aagcgtcagg acaggtagcg
     4021 gccgacataa atgatgattt caccaaaggg acgtatgaac catttctggg cggattatat
     4081 gcccagatat acaagatgcc gcaggcatcc aggctgaaga acaaccctga aaaactggat
     4141 tactatattc cgagaatcgg gcaccagctt acgctttata agatgggaaa cctgaaagac
     4201 tcgtacaagg tcgacagcat catcaataca aacttcaaga gcctcggcgc ttacaaatat
     4261 tcatgtttcg catggggaga ccagcgcata gaaaggatcg acacagacaa ccccaacggg
     4321 cgcaagctgc tcgtgataaa ggactcgtac gggagtgccc tggtaccgtt cctcgtacac
     4381 aactattcgc ttatctatgt catagatccc aaggggttca ataacgaggg atgcctggat
     4441 ttcgacgcca aggtactgat aaatgacgag ggtatagacg acgtgctgtt ctgtttctcg
     4501 atctacggaa gcggaaggaa gataatacag gacagcctga aaagcctgct gctgaggtga
     4561 ccgggtgtat aagaaaggac tcctgccctg ttcggcggga gtccttttat tattgctgct
     4621 tgtcagatcc atttatttat ctttgtattc cgtttgcctg tcaatacttg atatcagccc
     4681 tgtctgtcca ttgatgttga tatgacctgt ggtgtgcttt ttgcaggctg gcgttgtatg
     4741 caggcaatga cggactgccg gacggtactg ccgatgctgc tattccgcca gtaccgcttt
     4801 tagcttgttg atgtagtaac tgaggtcgct ttcaccgata tcgaggtcat cgactgaaat
     4861 gctgactatc tcgtatttga cctgtccggc cggagtactt acagtcacga tgctgccggg
     4921 tgcgttgagc aaaagcgcct gacccattgg cgacaggcag gatgcgtcaa acaggccggc
     4981 agaggatttc ccggcatatg gaagcacgat ccggcagttg atgatttccg aatcctcgag
     5041 gtcctttatc tgcactacgc ttccgattat ggaaaaagga catgaatcag cgccgtcttt
     5101 tttaacttct gcgtttttca ggaatttttc cagcctgttt atatactctc tgaaaaatgc
     5161 ttcgctctgc atcgcagtct gggcgttatc tgaaaaaagg ctcttgagta tcggttcctt
     5221 ttccctctct atttctgtaa tgtggtcggc caaaagcttg tacatccttt cggacagtgc
     5281 cgtttttcgc attttcatct ccctttcgat atttttgcat gcaaaaacaa acataagaag
     5341 acacttcgca ggccaaatgc gaaatgtctt cctccagtct ggcattgttc tttctaaata
     5401 tatgctaaca taaatgtaaa cacatgtcaa tgtaggttct cgctggaaga aagtgaaaag
     5461 aggaaagggg acactcctcc gcaagaggta ttcctttgtg gatgaggaat atcccctgaa
     5521 aaaagaaagg ggacgctcct tcacatgagg tgtttctctt cagttaagga gtgtccccat
     5581 atgatcaggg ttcctgtttc tggtattgca gaaggtacag cgaataatac atgccccgtt
     5641 tggccaggag ttcctggtgg gtaccggatt ccctgacctt tcccttgtgc agcactatta
     5701 tcttgtcggc gtgctggact gttgaaagcc tgtgggcgat cacgatggtg gtccggttcc
     5761 tggtgagctt ggcaagggcg tcctgtatca gcatttcagt ctccgtatcg atattcgagg
     5821 tcgcttcatc cagcaccagt attttgggat cgaatgccag agccctggca aatgcgagca
     5881 gctgcctctg tccggcggag aatgtggccc cgcgctccat cacttccgta tcgtaaccca
     5941 gcgggagttt gcttatgaat ccgtcggcat tgacatacct ggcggcttcc ctgactttat
     6001 catcagtgat gctttcctcg tcgagcctga tattgtcctt cagtttgccg ctgaacagga
     6061 agacatcctg gagcactgtc cctgtcagct tccgaaggtc atctttcctg atatccctgg
     6121 tatttatgcc gtctatcagg atctcgccct tctgtatttc ataaagcctg ctcaacaggc
     6181 ttatgatcga ggttttgcca gctcccgtcg cgccgacgat agcgacggtt ttgccggcgg
     6241 gcactgtaaa actgacatcg cgaaggaccc agtcgccttc attgtatgcg aaccacacat
     6301 tcctgaactc gatctcgccc ctgacgccgt ccggtaggct gagcgcaccg gagtcatctt
     6361 cggctggcgt gtcgagtatg aggaatatcc tttccgcggc tgccatcgag gcctgcagga
     6421 tgttgtattt ttcagcgagg tcattgatgg gctggaaaaa catgcttatg tagttaacga
     6481 atgcataaag cacaccgaac tgcagtgtcc cgtgcagcac ttcaaggccg ccgaaccata
     6541 tcagcagcga aagggctatg tatgacatca tttcaacggc agggcggaac aggccgaatg
     6601 ttatgatctc gctcataact gccctgtagt attcatggcc tatcctgccg aatctctcaa
     6661 agttctcctt ttccctgttg aacatctgga tgacccgcat ccctgatatg ttctccgata
     6721 tagcggaatt gatcttcgcc agcgatgtcc tgacgttcct gtacactttt cttatccttc
     6781 tcctgaaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcgccagcga
     6901 tgtcctgacg ttcctgtaca cttttcttat ccttctcctg aaaaataccg acagcactat
     6961 aacgaccggc attattgcaa gagaaaccag agtcatggtc acattaagtt tgaacatgat
     7021 caccacgacg cctgcgagta ttgctatatc tttcagcaac gtgacgagca cattggtgta
     7081 aacttcattc agcgcctcag tatcgttcgt cacgcgggta acgagtcttc cgacaggatt
     7141 tttgtcaaaa taggagaggg gcagcttctg catgtgcgaa aacaatttct gcctgatgtt
     7201 gaatatgatg gactggccgg catagctcag gacatatatc tgcagcaggt tgaacaggaa
     7261 gcctgagacc acgagcagca ggaaatatac tcccatcctg ctcagcgaag cagcatcgcc
     7321 tttctttatg aaatcatcga tggcaacgcc gatcaggtaa ggtcttgcca ggtctgcagc
     7381 cgtcgatgcg aacagaagta ttacggccag cgcgaacaga taccagtatc gggctgcaaa
     7441 tcccagcagc cttttcatca ggcggaagtc atataccttg acctggagct cttcttctgc
     7501 aaaaccttcg ttttccctgt tgtcatcagc catgctactc cgcctcctct atttcatctg
     7561 ccagaagctg tttcgtgtac aggtcatggt aatatccgcc ctgttcgaca agcgaatcat
     7621 gagtgcccct ttccatgatt tttccttcat cgaggactat gatctcgtcc gcatccctga
     7681 tcgccgatat cctgtgggat acgatgatgc aggtgcggtt tttcattatt gctttcaatt
     7741 cacggaggat gccctcctct gtttttgtgt cgaccgcgga caggcaatcg tccagtatca
     7801 gtatggaagg cgaccctata attgcccgcg cgatggctgt cctctgcttc tggcctccgg
     7861 acagcgtgac gcccctttct ccgacgagcg tatcgaattt ctgcgggaat tccatgatat
     7921 cgtcatataa gcgggccatt tttgctgctt tttctatttc ttccatactg aagcccctgt
     7981 agaaatcgat gttgttcttt atcgtgtctg aaaacaggaa cgtgtcctgc ggcacgcatc
     8041 ctatattgcc tctcagagaa gacagcgata tatcgtttat atcaatgccg tttatgaaca
     8101 acgtgccttc tggagccgac aggatcctcg ggacgaggtt tatcagggtc gacttgccgc
     8161 tgccggtttt gcctacaatg gccagtgttt tgccgtgatc caccgaaagg ctgatatctt
     8221 tcagcacagg ttccctgctg cccgggtact tgaatgtcag gttcctgaat tcgatggctt
     8281 tggtccccac ggcgccgtcc ggattcttcc cgctgacgtc tgctttttcg gatacatgca
     8341 gcgcggcggc accggggatc accagcgtta cggctcctgt tttgtccgct atttccggtt
     8401 tttcatccat cagtgcattt atcctgtcca gcgacaccga accgcgtttg aatatgttgg
     8461 ctacccatcc gacagcggtg atgggccaga tcagcatccc gagatagctg ttgaatgcta
     8521 cgaactctcc gagcgatacc gctccgtcga tgaccagcag gcctccatac cagagcgcta
     8581 tgacgaaaga aagagatgat accagcatca cgctggggaa catcatgctc atgatcctga
     8641 tgaacctgag gttggcacgc ctgtttgctt cgttgacgct ttcgaagcgt tctgtctggt
     8701 ggttctcctg cacgaacgat ttgatgaccc ttatccccga gaaattctcc cgggctgcct
     8761 ccgtcaggtt tgagaaggct tcctgctgcc tttggatggt gctttgcatg attctcatat
     8821 atgaaaacac caggatgccc aggacaagga tcggtgcgaa gctggccgcg gtcagcctga
     8881 ggccgcctgt ctggagcatc atcacaaggg aagcgacggg catgagcagg caatccacgg
     8941 caaatatgac tccctgtccc gtggccatcg tgacgttgtt catgtcattc gtcacatgag
     9001 ccatgatgtc tccggtcttg tgcgtattga aataattggc cgaaagcttc tgaaggt
//
LOCUS       DAOSCC010000092         9023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5052_length_9023_cov_3.995986, whole genome shotgun sequence.
ACCESSION   DAOSCC010000092 DAOSCC010000000
VERSION     DAOSCC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5052_length_9023_cov_3.995986"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLG72_06280"
     CDS             <1..715
                     /locus_tag="PLG72_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ80439.1"
                     /translation="DFDSYRQWLEEIGVGTGLVRIREDDFTASCFINTDLSGNQITGF
                     YPGAMAYDEKISMLEIPMDDVSMVIIAPTEPAAMSRWCDELQKIGIPYLYDAGMQIPR
                     LEPDDLAKGILGARIVIFNEYEYAMMRKKTGLTMGDILAGAELAVITLGEKGSELWTK
                     DGRVTVPAAKPVRVVDPTGAGDAYRAGLLKGYFEGASLEKMGKYASVTAVYAVEHKGA
                     TEHRYTMDEFYARYRENFE"
     gene            1059..2321
                     /gene="ahcY"
                     /locus_tag="PLG72_06285"
     CDS             1059..2321
                     /gene="ahcY"
                     /locus_tag="PLG72_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ80440.1"
                     /translation="MVKCDIRDKSLAPKGKLRIEWADNQMPVLRMIRERFEKEKPLAG
                     KKMSCCLHVTTETANLMRTLKAGGADIVLCASNPLSTQDDVAASLVVDYGISVFAMKG
                     EDTETYYKHLKAAIEHGPDITQDDGCDLVGLLHKEYGDSYLKNVIGGTEETTTGVIRL
                     RSMEKEGALRIPIIAVNESDTKHLFDNRYGTGQSTIDGLLRATNILLSGKNFVVCGYG
                     WCGRGLAMRAKGMGANVTVTEVDYVKAIEAVMDGFRVMKLEDAMSFADIIITVTGDLN
                     VVDEKHIKLAKDGVIIANSGHFNDEINIDALERLSVSKRTVRDFVEEYTLKDGRRVYL
                     LAEGRLLNLAAAEGHPAVVMDMSFANQALGAEYLVRNAHKMEKKVYVLPTELDQEIAR
                     LKLESMGITIDELTEEQIAYLSSWQSGT"
     gene            2631..3326
                     /gene="deoC"
                     /locus_tag="PLG72_06290"
     CDS             2631..3326
                     /gene="deoC"
                     /locus_tag="PLG72_06290"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ80441.1"
                     /translation="MKTQDVKAIASMIDHTILKADATKEQVAEYCRQAIKYGFASVCV
                     NPCHVPQVRKELQGSGVKVCTVIGFPLGADSIETKAFAAKTAVRDGADEIDMVINIGA
                     VKDGDFDYVEKEIGEVVASSGDALVKVIIETCFLTDTEKVLACKAAAAAGAGFVKTST
                     GFGTAGATAEDVRLMKETVGKNVGVKASGGIRSYEAALEMIEAGADRIGTSSGVKIME
                     EALDKSNATTSQK"
     gene            3418..4725
                     /locus_tag="PLG72_06295"
     CDS             3418..4725
                     /locus_tag="PLG72_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ80442.1"
                     /translation="MRILIEGIDVLTADPAAEYIRNADIGIADDRIEFIVPAGQRVPD
                     FIPDRKINGKNMLAMPGMVNAHTHCAMTLMRNAADDLPLHKWLFEKIFPIEDRLTDDD
                     VYWGTRLAAAEMIKSGTTALADMYLHMDAAARGIVDTGMRVNLSKSPLEFHSEGGLKA
                     DDVFDDVRTYFKNWNGKYNGRIRVYLEVHSTYLFDWPSLIRASELAKELGTGIHIHLL
                     ETARERKESFEKHEKSSVEICAETGIFDVPVIGAHLVHIDEEDIRILKQYGVNAAHNP
                     TSNLKLGSGISPVHKMLDAGVNVALGTDGTASNNNLNMFEEMHLAALIHKGVAQDPEL
                     VTARQAFMMATVNGSRAVGFGDETGILKPGMKADIILLDMDKPHLCPVNDPFSAVVYS
                     AQASDVDTVIVDGNILMEKRELKTIDEERVMFEVRKHSGMLLE"
     gene            5357..6592
                     /locus_tag="PLG72_06300"
     CDS             5357..6592
                     /locus_tag="PLG72_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOJ80443.1"
                     /translation="MQRYAVLMAGGSGTRLWPLSKEASPKQFIPMEGDSSMLVQTIKR
                     LCRVVQPENCFVVTNRSLADITKETAGRLIPDENIILEPDRKNTAACIAYTTLLLKKK
                     LGAGILCFVPADGYVKDTSGYARALRMAFDTAENVDSLVIIGVKPAYPATGYGYIHVE
                     PVKRKKSAGEDTAANINSSPDEQSATAEGSTPGSKSDAEKSTLPGEESASFADSAISY
                     ENIDILRVLEFIEKPALKTAQKLAASDDYLWNCGIVVGSMDSIIQKIKENIPEHYHKL
                     SNALESGEYDEGELIRNAYGELHSISFDNAVLERCAASLYAVRASFDWDDIGSIDALA
                     MTLEPDSDGNLVKGRHIGINTTNSVIYGKDAAICTIDIDNMIVVGTKDEVLVCPRNKS
                     QKVKLLVEKLKEQGHDDLL"
     gene            6639..>6886
                     /locus_tag="PLG72_06305"
     CDS             6639..>6886
                     /locus_tag="PLG72_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80444.1"
                     /translation="MANTGFILIREIRAGMWNELHHVLTQSLIAEILQRAPGVYWGKG
                     SLYASPDGSNVFDQFFLPVSVFDVHDLVKEGYTFYPEY"
     assembly_gap    6887..6896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7159..7764
                     /locus_tag="PLG72_06310"
     CDS             7159..7764
                     /locus_tag="PLG72_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-fucosyltransferase family protein"
                     /protein_id="HOJ80445.1"
                     /translation="MAECGADVIVCDTYIDVENVIPYIPEWHPLCGMNRRDLFAHLIR
                     KYIRLQKDVRDFVDDFYNSYMRDAAFLAVHIRGSDKIVEVRNLHDLNGRYADEIDRVL
                     NANPGMKLFLMTDCIEILEEYKQRYGDLLVHTDCRRTHTDGLGIHFLECPDNKLKGLE
                     IIRDTWLAARCDYFIGNGYSNVSAAISELKDWEDGRIRLLY"
     gene            7797..8762
                     /locus_tag="PLG72_06315"
     CDS             7797..8762
                     /locus_tag="PLG72_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOJ80446.1"
                     /translation="MEKSKWLEAYRTMRLIRLIEQRINDEYKHDEIKTPIHLSIGQEA
                     AAVGVCMNLRKDDYAFGTHRSHAMYIAKGGDIKKMFAELYLRKTGCARGRGGSMHLAD
                     TEAGIPGSSAIVGGNIPLGTGTALASKILDNDRVTVVFFGDGAADEGTFHESLNFASV
                     KKLPVVYVCENNFYAVNSHQMARQAGDNIYKWAQGYGMPGFRIDGNDVLEVSEYAKEA
                     IDRCRNGGGPTLLECLTYRWKGHIGTTDDVGESYRPKEEYDYWISRCPIKRFSDFLRN
                     EGVLTDELINSIDREILDLIEEAFQFAQNSPKPSPDELLDFVYAD"
BASE COUNT         2609 a         1836 c         2506 g         2062 t
ORIGIN      
        1 cgactttgac agctacaggc agtggcttga ggagataggc gttgggacgg gactcgtgag
       61 gatcagggaa gatgacttta ccgcttcctg tttcatcaac accgaccttt ccggaaacca
      121 gatcacaggg ttctatccgg gcgcaatggc gtatgatgaa aaaatcagca tgctggaaat
      181 accgatggac gatgtttcaa tggtgataat agcgccgacc gagcccgccg cgatgagcag
      241 atggtgtgat gaattgcaga agatcgggat accatatctg tatgatgcgg gaatgcagat
      301 cccgcggctg gagccggatg atctggccaa aggcatactt ggagcaagga tagtcatttt
      361 caatgagtat gagtatgcta tgatgcggaa aaagacgggc cttaccatgg gcgatatact
      421 tgccggggcc gagttggccg tgatcactct tggagaaaaa gggtccgagc tgtggacgaa
      481 agatggaaga gtgacggtcc ctgcagccaa accggtcaga gttgtcgatc cgactggcgc
      541 aggcgatgct tacagggccg gcttgctgaa agggtatttc gaaggggcaa gccttgaaaa
      601 aatgggcaag tacgccagcg ttacagcggt atacgctgtc gagcacaagg gcgcgacaga
      661 acacaggtat acgatggacg agttctacgc gaggtacagg gagaactttg aataagggac
      721 agttctccga aaaccggctg atgaggggac ggttctcagg aactggggac gaactgggga
      781 cagttctcaa aaattggcct tagggacgaa ggctttatag gggacagttc tcaataaaga
      841 gaaggctaag gaaaagcgcc ggtcaccggc attggagccg aacatttcat aaaaagatac
      901 tgcccgggaa gccgacagtc catgaaaatt ggatgagtct tcagaataag ggaattgcct
      961 gaaagggaca gttctcaaaa aactgacaga atcgacaagg agttaaagaa ttaatataac
     1021 atacaacagg cttgtatatt tcacaggagg tattttaaat ggttaagtgc gacattaggg
     1081 acaaatcatt ggcgccaaag ggcaaattga ggatcgaatg ggcggacaac cagatgccgg
     1141 tactgagaat gatcagggaa cggttcgaaa aggaaaagcc ccttgcaggc aaaaagatga
     1201 gctgctgcct gcatgttact acggaaacgg cgaacctgat gcgtacgctt aaagcgggag
     1261 gagcagacat agtcctgtgc gcatcgaacc ctctgtcgac ccaggacgat gtggcggctt
     1321 cgctggtcgt tgattacggc atatcagtgt ttgccatgaa gggcgaagac accgaaacgt
     1381 attacaagca tctgaaggcc gccattgaac atgggccgga tataacgcag gacgacggat
     1441 gcgatcttgt cggccttctt cataaagaat acggcgacag ctatttaaag aacgtgatag
     1501 gcgggaccga ggaaaccacg acaggcgtca tcaggctcag gagcatggag aaggaagggg
     1561 ctctgcggat accgatcatt gctgtgaacg aatccgatac aaagcacctg ttcgacaaca
     1621 gatatggtac aggccagagc acgatcgacg gacttctcag ggctacgaac attttgctgt
     1681 ccggaaagaa tttcgtggtc tgcggttatg gctggtgcgg cagaggtctt gcaatgaggg
     1741 caaaaggaat gggtgcgaac gttaccgtga cagaggtcga ttatgtcaag gcaatcgaag
     1801 cagtcatgga cggattcagg gtcatgaagc tcgaagatgc catgagcttt gccgatatta
     1861 tcatcacggt gacaggcgat ttgaacgtgg tggacgaaaa gcacatcaag cttgcaaagg
     1921 acggagtgat tatagccaac tccggtcatt tcaatgacga gataaacatt gacgcgcttg
     1981 agaggctttc tgtatccaag aggacggtgc gtgattttgt ggaggaatat acgcttaagg
     2041 acggacgcag ggtatacctg ctggcggagg gaaggctcct gaacctggca gccgcggagg
     2101 gccatcctgc tgtcgtgatg gatatgagtt ttgcaaacca ggctttgggt gcggagtacc
     2161 tggtcaggaa tgcgcataag atggaaaaga aggtatacgt tcttccgact gaactggacc
     2221 aggagatcgc tcgcctcaag ctggaatcaa tggggatcac cattgatgaa ctgacagagg
     2281 agcagatagc ttacctgtcg tcatggcaga gcggtacgtg agtgtacggt gtcacatgta
     2341 taaaatgcgg ttcagtatat gattgcagta taaatatcta tgtctgagcg gtacacgaat
     2401 acacattggc aaacgaaaaa agtacggtct taatgcgcag aactggaagc tgacagatgc
     2461 ccagaacttt gaagaagtca ttgacgcatc tgtcagcagc gtgtatcagg acaattgcaa
     2521 atagacgggc agatatcacg tgatgctgga ccgagtgaag attgcttata atgtgcttca
     2581 gaaatacgat aaactcatgc tccgaatact tgcatgaatg gtgaggacag atgaaaacgc
     2641 aggatgtcaa agcaattgct tcaatgatag atcatacgat acttaaggca gacgcgacaa
     2701 aagagcaggt ggccgaatat tgcagacagg ccataaaata tggttttgcg tctgtctgcg
     2761 tgaatccgtg ccatgtgccg caggtcagaa aagaactgca gggttcaggt gtcaaggtat
     2821 gtaccgtgat cggattccca ctgggagccg acagtatcga aacaaaggcg ttcgcagcca
     2881 aaacagctgt gagggacggc gctgatgaga tagatatggt catcaatatc ggagcagtta
     2941 aggatggcga tttcgattac gttgaaaaag aaatcgggga agtcgtggca tcatccgggg
     3001 atgctttggt aaaagtcatt atcgagacct gctttttgac tgataccgaa aaggtattgg
     3061 cttgcaaggc agctgccgct gccggagcag gatttgtgaa aacgtcgacc gggtttggca
     3121 ctgccggggc cacggcagaa gatgtcaggc tcatgaagga gactgtgggg aaaaacgtcg
     3181 gagttaaggc ttccggcggt atcagaagct atgaagcggc acttgaaatg atcgaagccg
     3241 gagccgaccg tataggcacc agttcaggag ttaagataat ggaagaagcc ctggataaaa
     3301 gtaatgcgac tacttcacag aagtaataag aaacctgagg aaaataacga tcaaaaatag
     3361 caagaaagtt tccgaataaa gtaaaggaag cttcttgata aggaatggga gagtaatatg
     3421 aggattctta ttgaaggtat tgacgtcctg accgccgatc cggcggcaga atacatcagg
     3481 aatgcagata tcggaatagc agacgacagg atagagttca ttgtgccggc aggacaacgt
     3541 gtaccggatt tcatacctga caggaagatc aacggtaaaa acatgctggc aatgcccgga
     3601 atggtaaacg cccatacgca ctgtgcaatg acgcttatgc gcaatgcggc ggatgatctg
     3661 cctcttcaca aatggctttt cgagaaaatt tttccgattg aagacaggct gacagatgat
     3721 gacgtatact ggggaacacg cctcgcagct gcagaaatga tcaaatccgg tactactgct
     3781 cttgctgata tgtacctgca tatggatgct gcggccaggg gtatcgttga taccggaatg
     3841 agggtaaatc tgtcgaagag cccgctggaa tttcactcag aaggcgggtt gaaggccgac
     3901 gatgtatttg atgatgtcag gacttatttc aaaaactgga acggaaagta taacggcagg
     3961 atcagggttt atctggaagt gcactccaca tatctctttg actggccttc acttatcaga
     4021 gcatccgaac ttgcaaagga gctgggaacg ggaatccata ttcacctgct ggagactgcc
     4081 agggagagga aggaaagttt tgagaagcat gaaaaatcgt ctgtcgagat atgcgccgaa
     4141 acagggatat tcgatgtacc tgtaatcgga gcccatctgg tccatattga cgaggaagat
     4201 ataaggattc ttaagcagta cggcgtcaac gcggcacaca acccgacaag caaccttaag
     4261 ctaggaagcg ggatatcgcc ggtccacaag atgcttgatg ccggggtcaa tgtcgctttg
     4321 ggtaccgacg gcacggcaag caacaataac ctgaacatgt tcgaagaaat gcatcttgct
     4381 gccctgatcc acaagggagt agcccaggat cctgaactcg taactgcccg ccaggcattc
     4441 atgatggcga ccgtgaacgg gtccagagcc gtcggcttcg gtgatgagac tggtatcctg
     4501 aagccgggga tgaaagcaga cataatattg ctcgatatgg acaagccgca tctgtgtcct
     4561 gtaaacgacc cgttttcagc agtagtctat tcggcacagg cttcagatgt cgatactgtg
     4621 atagtggatg ggaatatatt gatggaaaaa agggagttga agaccatcga tgaagaacgc
     4681 gtcatgttcg aggtcagaaa acactccggt atgctgcttg aataagcaaa atttcgccgg
     4741 gacggtcaat ttacaagcag gtcaaattgc gttcatagag gggtgccagt taaaatcaaa
     4801 aacttgtacc aggcagaaat gcttcatttt gtgaagatgc tgcaagcaaa caactctgtt
     4861 tatcaaactt ggatcagcgc atgaagggtt ttggacagag caggcagacc cttgtaagga
     4921 agtattactg aaaatcttta gaaattggac ggcacagtcg aaaggctgtg ccattttgct
     4981 tcaggcaact ctattctcat gtcagattaa cctgaacgat cagacagaat tatgaatgag
     5041 ttaacggtgt gtatcatatg atacgtctct taaaggaacc ctaatattca aatttacata
     5101 atatgaacta tgcattatgt attatacaat ttcctggtat atacgttgat tgtttcaaaa
     5161 gtcgcgctgc ctgcttcaca gaaagctttg agtctgtctg cgaaggcttg cggtctgcgg
     5221 catgcaacct ttttcgaaag ggggataggc tgtccgggaa tgcacatgat ccactggaag
     5281 gtacataatt gaggccatgc atttgtcact acataagctt atttttaaaa atgtattgac
     5341 cagggagtgc aggaatatgc agagatatgc cgtacttatg gcaggtggtt cgggaacgag
     5401 gctctggcct ctttcaaagg aagccagtcc gaaacagttc atccccatgg aaggggacag
     5461 ttctatgctg gttcagacga tcaagcgctt atgcagggtc gttcagcccg agaattgctt
     5521 tgtcgtgact aaccgcagtt tggccgatat aacaaaagag acggccggca gactcatccc
     5581 cgatgaaaat atcatcctgg agccggacag gaaaaatact gcggcatgta ttgcttatac
     5641 gacgctgctg ctgaagaaaa aactgggtgc gggcatactg tgctttgtgc cggccgatgg
     5701 ctatgtaaaa gacacgtcag gttatgcgcg ggctctccga atggcgttcg acactgctga
     5761 aaatgtcgac agcctggtca taatcggtgt caaacctgcc tatccggcaa ccgggtatgg
     5821 ttacatccat gtagaacctg taaaaaggaa gaaatcagca ggagaagata ctgcagcaaa
     5881 tataaattca tcgccagacg agcaatctgc aacagccgaa ggttcaacac caggatcaaa
     5941 atctgatgca gaaaaaagta cattaccagg agaagaatct gcttcatttg ctgattcggc
     6001 tatatcatac gaaaacattg atatattgcg ggtccttgaa ttcattgaga aaccggcact
     6061 gaaaacggcc caaaagcttg cagcttcgga cgattatctt tggaactgcg gtattgtggt
     6121 cggttctatg gattccataa ttcagaaaat aaaggagaac atccccgagc attaccataa
     6181 gctttcgaat gctttggaaa gcggagaata tgacgaaggc gaactgatcc ggaatgcata
     6241 cggtgaactg catagcattt cgtttgacaa cgcagtgctt gagagatgtg ctgcttcact
     6301 gtatgctgta agagcttcct tcgactggga cgatatcgga agtatcgatg ccctggctat
     6361 gactcttgag cctgactccg acgggaatct ggtgaaaggg cgacatatag ggataaatac
     6421 tacaaattcg gttatttacg gtaaagatgc tgcaatttgc acgatagata tagacaacat
     6481 gatcgtggta ggaacaaagg acgaagtact ggtctgcccc aggaacaagt cgcagaaagt
     6541 aaagctgctg gtggaaaagc tgaaagaaca ggggcatgat gatctcctgt gagaaaatgg
     6601 acttttaccg tgcaagacag acttttggag ggatactgtt ggcaaatacg gggttcatac
     6661 tgataagaga gataagagcc ggtatgtgga acgaactgca tcatgtgctg acccagtcac
     6721 ttattgcgga gatccttcaa agagcccctg gggtttactg gggtaaaggc agcctttatg
     6781 cttcacctga cggttccaat gtgtttgatc agttttttct gcccgtgtcc gttttcgacg
     6841 tacatgattt ggtgaaagaa gggtatacgt tttatcctga atactgnnnn nnnnnngaat
     6901 gtatcgaata tactgtcctt gtgtccaggg tatgatagcc ggaatgattt gtgtattggt
     6961 ttttcatatc ctgaaaacag aaccttggca tgtttcagtc cgtataagaa taattctccg
     7021 gcacatgatt cagaggacag tagtttggac atttcaaata tcttgttgcc cgatccttgg
     7081 aatgatgact gcgatagtat gaaaaatata ctgtacaaca gtcaaagcaa cgggtatgaa
     7141 aacaggttgc cggatcactt ggcagaatgc ggtgctgatg tcatcgtctg cgatacctat
     7201 atagatgttg aaaatgtcat tccttatata ccggaatggc atccgctctg cggcatgaac
     7261 cgcagggatt tgttcgctca tctgatccgg aaatatatac ggcttcaaaa agatgtccgg
     7321 gactttgtcg atgactttta taactcatat atgagagatg ccgctttcct ggcagtgcat
     7381 atacgaggca gcgataaaat cgttgaagtt cggaatctgc acgacttgaa cggcagatat
     7441 gcggatgaaa ttgatagagt gcttaatgcg aatccgggta tgaagctgtt cctgatgacc
     7501 gactgcattg aaatcctgga agagtataaa caaaggtatg gggatctttt ggtccataca
     7561 gactgcagaa ggacccacac ggatgggttg gggatacatt tcctcgaatg tccggacaac
     7621 aaactgaagg ggttggaaat catcagggat acgtggcttg ctgccaggtg tgattatttt
     7681 atcggcaacg gatattccaa tgtttcggca gccatcagtg agctaaaaga ctgggaggat
     7741 ggcaggatca ggcttttgta ctaactgaaa gatgcgatga ggagaggcgg tggatcatgg
     7801 aaaaaagcaa atggctcgaa gcctacagga ctatgcgtct catacgtttg atcgaacaaa
     7861 ggatcaacga tgaatataag catgatgaga taaaaacgcc gatacatctg tccatcgggc
     7921 aggaagcagc ggcagtcggc gtttgcatga acctgcggaa agacgattac gcatttggca
     7981 cccatcgaag ccatgcgatg tatattgcaa agggcggaga tattaaaaaa atgtttgccg
     8041 aactttacct gagaaaaacc ggatgtgcgc gcggcagggg cgggtccatg caccttgccg
     8101 ataccgaggc aggcataccc ggctcttcag caatcgtagg agggaacatc ccgctgggga
     8161 ccggtacagc cctggcatcg aagatcctgg ataacgacag ggtaacggtc gtgtttttcg
     8221 gcgacggcgc cgcagacgaa gggacattcc atgaaagcct gaattttgct tcagtaaaaa
     8281 agctgcctgt cgtgtatgtg tgcgaaaata atttctatgc cgtcaactcg caccagatgg
     8341 cgcggcaggc cggagacaac atttataaat gggcacaggg ttacgggatg cccggtttcc
     8401 ggatcgacgg aaacgatgtg ctggaagttt cagaatatgc gaaggaagcc atcgacagat
     8461 gccggaacgg tggaggccca acactgctcg aatgcctgac ctacaggtgg aaaggtcaca
     8521 taggaacgac ggacgatgtg ggcgaaagct accggccaaa ggaggagtat gactactgga
     8581 tttcgaggtg cccgataaag cgcttctctg attttctaag gaatgaggga gttttaactg
     8641 acgaacttat aaacagtatc gaccgggaga tactcgacct gatcgaagaa gccttccagt
     8701 ttgcccagaa ctcccccaaa ccttcgcctg atgaactgct cgatttcgtc tacgccgatt
     8761 aggttgggga cagttctgca gaacttgagt tgggggacag ttctgcggaa cttgagtcgg
     8821 gggactgagt ttggggacag ttctgcataa agtatagtta cagtccgatg atggagcaac
     8881 tgatgaaaac tgacttgccg aaaggaggat tattatgccg tggacgacgg ttcaagtgga
     8941 aaaactcgat gactatttca ttaaaggcga aactgagggc gatcgtgtca tcacgtacaa
     9001 agatgcgctg catgaagccc tgg
//
LOCUS       DAOSCC010000093         8928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5122_length_8928_cov_4.996506, whole genome shotgun sequence.
ACCESSION   DAOSCC010000093 DAOSCC010000000
VERSION     DAOSCC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5122_length_8928_cov_4.996506"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2405)
                     /gene="yicI"
                     /locus_tag="PLG72_06320"
     CDS             complement(57..2405)
                     /gene="yicI"
                     /locus_tag="PLG72_06320"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HOJ80447.1"
                     /translation="MKFSNGYWLLRDGVTLASPVEIRDTRICDGILKLFVATRPVRHR
                     GDTLDGPMLTYEFFSPFEDVIGVKVYHFKGGLPKSLKFELYRKEGYSPVITQDDEYIT
                     MTSGRLAMRLRKTGGWSMDFLYDGKRITGNGQRNTGYLTVAGEGAYMREQLDLGVGEY
                     IYGLGERFTPFVKNGQVVDIWNEDGGTSSEQAYKNIPFYISSRSYGVFVNHTGKVSYE
                     IGSETVSKVQFSVPGESLEYFVIGGAAPKDVLSNYTLLTGRPALPPAWSFGLWLTTSF
                     TTSYDEETVTSFIEGMEQRDIPLHVFHFDCFWMKESQWCDFTWDRDVFPEPEAMLKRL
                     KERGLKICVWINPYIAQKSHLFDEGMENGYLLKRPDGTVWQWDRWQAGMGIVDFTNPD
                     AVEWYKDKLRKLLDMGVDCFKTDFGERIPTDVVYHDGSDPVLMHNYYTYLYNKAVFEV
                     LEEKRGRNEAVLFARSATAGGQKFPVHWGGDCSANYDSMAETLRGGLSLCLSGFGFWS
                     HDISGFERTAPPDIYKRWTAFGLLSSHSRLHGNESYRVPWLFDEEAVDVLRFFTKLKC
                     RLMPYIYSAACEAAYKGIPTMRAMFLEFPDDPTCLYLDRQYMLGESLLVAPIFNNEST
                     VQYYLPAGKWTNVISGEKIEGRSWRTEKHGYMSLPLLARPNSVIPFGKIDDRPDYDYA
                     DGVEFHIFELSDGACADSVIYDTNGRRDVVFKASRKGNVISMEAAGVSAGTGAGGNDR
                     QWFAVLRGIEKVSSVEGGRAEATGKDVRIIPDKGIRTVKAVL"
     gene            2566..3456
                     /locus_tag="PLG72_06325"
     CDS             2566..3456
                     /locus_tag="PLG72_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80448.1"
                     /translation="MDKLLLKEDRVHGDVSFPLGSYYMEIPSGSVVLDCHWHEEMELL
                     LVTSGKAQFRIEAEYYEVHQGECIFVNSGELHAGFQIDKSPCSYKALVFSPSLLYSSS
                     FDLTRTNYVDPIMKNSLIVKRHITASPGWQSELLGRLEMLLRYLDARPPAYELLVKSE
                     LFGIFALLSANCISSANTPERSQDHFRLEKLKTALKYIQDNYSKKISTFDIAASVDMS
                     EGHFCRLFKHYVKKTPVEYLNYYRITRAARLLEETGMKVLEVAMEVGFDNLSYFIGTF
                     KRHMGMTPSKYRSLRGKAML"
     gene            complement(3634..5988)
                     /locus_tag="PLG72_06330"
     CDS             complement(3634..5988)
                     /locus_tag="PLG72_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ80449.1"
                     /translation="MKFGYFDEAANEYVITRPDTPTPWINYIGSGKYGGIVTQTGGGY
                     SFHKDPQNRRITRYRYNSIPMDRPGRYIYIRDAATGEYWNPGYQPSCTKLDSYLCRHG
                     MGYTVLEGEYRGITASLTYFVPDDRDFEIWLLKVRNTLNVPKKLQIFSYAEFCFWDAV
                     MDQQNVDWVQQINQCRYNDGIITWYPHFVSGSASFFATGAEVSSYDCNLETFIGRYRS
                     ESNPIAVEQGACSNSISRRTNGVGAFCNEIELGRGETKEIVFILGYTDNRDGIKKEIQ
                     PYLDPAEAKAALGRLNEYWKDYISKLHVDTPDREMNLFVNMWNQYQCKTTFNWSRFVS
                     MYQLGLGRGMGIRDSAQDTLGVMHTIPEEAKGLIIKLLQCQYTDGRAYHLFFPLTGEG
                     GVGDAPIKKFDWYSDDHLWLILAVNAYVKETGDFAFLDQKVLYNDKKTEGTVWEHLVK
                     ALEFTDTWKGPHNIALAGRADWNDTLNLDTGKGVAESVFTSMLFCRAANEMEELAVFL
                     RRNEDAVRFRDMSEKMKKAINDTCWDGEWYVRAFDDESKPLGSKENTFGRIFINSQSW
                     AILGGVADKERARLCLESVNKYLNTKYGIVAMYPSYTQHDPTKGGVTTYPPGAKENGG
                     IFCHTNPWVMIAEIMEGNGDRAFEYYNQILPARRNEDADHYEVEPYVYAQNILGKEHP
                     QFGIGRNSWLSGTAAWNMVASSQYILGIRPGYDCLTVDPCIPSSWDGFRARRIFRGAE
                     YEIEVRNPSHVCCGVKKIILNGKEVDRIPVQPAGSKSQVIVELG"
     gene            6235..6648
                     /locus_tag="PLG72_06335"
     CDS             6235..6648
                     /locus_tag="PLG72_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80450.1"
                     /translation="MSAYVNISKELRNFLGTNKIFRYILPLDVVIMFAGLILIFLDDT
                     VGVNIGGFLRALVHWTFITGLLLTYANMKERPLYIGLIGYGAIHLISFLRLLFKYGYF
                     DCSDLFGSAVYAGLGYLVLRKALAGSADKTPNTAG"
     gene            6930..7754
                     /locus_tag="PLG72_06340"
     CDS             6930..7754
                     /locus_tag="PLG72_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ80451.1"
                     /translation="MKSRVFITGATGGLGKAFAVECASRGSDLFLTDISDASLAKLSE
                     SLANTYGIKVSFYSCDLTDAGSRAGLYGHIRSRCLMFDFLINVAGLDFEGAFAERTPD
                     QIRTMVRLNIEANLELTHELLKLRDPARTFRIINVSSLAAFYPMPVKAMYSASKRFLL
                     NFSRALGEELRSRNVTVTALCPAGMPTTPECIEAIEAQGLAGRITTCNVGYVASKTID
                     MALRGKQVYIPGLLNRILRAAGSVVPDSIIARAVGSRWNAAHTRRMRRHGTNKAYT"
     gene            7772..8689
                     /locus_tag="PLG72_06345"
     CDS             7772..8689
                     /locus_tag="PLG72_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ80452.1"
                     /translation="MENSNDTIKTIVITGATSGIGLAAAKELAMKGAYVIGTGRSEER
                     CRYAEQEVKSVCPDANITYLAADLSSLSEVRRLASDIRETLAKDGFSQLDVLINNAGT
                     VSSWYISTVDGFELQFAVNHLAPFLLTNELLPLLGRSRAGTVICTSSGSHYRTRLRWN
                     DIMLRKHYNTLLAYKQTKLANVLFVTELNRRLAASGSNIKAFALDPGLVDTDIGFKGT
                     SGIAKLVWKFRSKGGTSPEVPAHSMAYLALDPEPRSTGKVYWKDCRPKEPSRYSQKPD
                     VAKKLWELSERMCGIIGHKSSQEQKQTAV"
     gene            8708..>8928
                     /locus_tag="PLG72_06350"
     CDS             8708..>8928
                     /locus_tag="PLG72_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ80453.1"
                     /translation="MDAQMKKGLLDACVLHILTKGDTYGYKLTREITDLMDVSESSLY
                     PVLRRLETQGFLETYSAEHSGRLRRYYRIT"
BASE COUNT         2064 a         2450 c         2215 g         2199 t
ORIGIN      
        1 tcgatccggg caaaacaccc gccggaataa acaggactca caaaacgcgg atcatatcac
       61 aaaaccgctt tgacagtccg gattcctttg tcaggtatta tcctcacatc tttacctgtc
      121 gcttcagcgc gtcctccttc gaccgaggac actttttcga tgcctctcag cacggcaaac
      181 cattgcctgt cattgccgcc ggcgccggtt cccgccgaaa cgcccgcagc ttccatactg
      241 atcacgttcc cttttctcga tgctttgaat accacatccc tgcgtccatt ggtgtcgtaa
      301 atcactgaat ccgcacaggc tccgtcggaa agctcgaaaa tgtggaattc cacgccatcc
      361 gcataatcat agtccggcct gtcatcgatc ttcccgaacg gtatgacgga attcggcctt
      421 gcaagcagcg gaaggctcat gtaaccatgc ttttcggtcc tccagcttct gccctcgatt
      481 ttctctccgc tgataacatt ggtccatttt cctgccggca aatagtactg taccgtgctt
      541 tcattattga atatcggcgc caccagcagc gactctccca gcatgtactg cctgtcaaga
      601 tacaggcatg tcgggtcatc cgggaattcg aggaacattg ccctcatcgt cggtatccct
      661 ttgtatgccg cttcacatgc agcgctgtag atataaggca tcagcctgca cttcagcttc
      721 gtgaaaaacc tcagcacatc caccgcttcc tcgtcaaaca gccagggcac cctgtaggat
      781 tcgttgccgt gcagcctgct gtgtgaagag agcagtccga aagcggtcca gcgtttgtag
      841 atgtccggag gcgcagtcct ttcgaagccg cttatgtcgt ggctccagaa accgaaaccg
      901 gacaggcaca gcgacaggcc gccgcgcagg gtctcagcca tggaatcata attcgccgag
      961 cagtcgccgc cccagtgtac cgggaatttc tgcccccctg cagttgcaga acgtgcaaag
     1021 agcaccgctt cgttccggcc gcgcttttct tccaggactt caaacacggc cttgttatac
     1081 aagtaagtat agtaattgtg catcagcacc ggatcggaac cgtcatgata gaccacatcc
     1141 gtcggtatcc tctcgccgaa atcggtcttg aagcagtcga cgcccatatc gagcagcttc
     1201 ctgagcttat ccttgtacca ttccacggcg tccggattcg taaagtcgac tatgcccatt
     1261 cccgcctgcc acctgtccca ctgccagaca gtaccgtcag gcctcttcag caggtacccg
     1321 ttttccattc cctcgtcgaa caggtgcgat ttctgggcaa tgtacgggtt gatccatacg
     1381 catatcttaa ggcctctttc tttcagcctc ttcagcatag cttcaggttc cgggaataca
     1441 tccctgtccc atgtgaagtc gcaccactgg ctttccttca tccagaagca gtcgaagtgg
     1501 aaaacgtgca gcggtatatc gcgctgctcc atgccctcga tgaagctggt cacagtttcc
     1561 tcgtcgtagc ttgtggtaaa ggatgtggtg agccaaagtc cgaatgacca cgccggaggg
     1621 agcgccggcc tgccggtgag cagcgtgtag ttggaaagca catccttcgg cgccgcgccg
     1681 ccaatgacaa aatactccag cgattctcca ggtacgctga actgcacctt cgaaacggtt
     1741 tccgagccta tttcatagga cacctttccg gtatggttga caaacactcc atagctcctg
     1801 ctggagatgt agaacgggat atttttatat gcctgctcgc tgctggtgcc tccgtcttcg
     1861 ttccagatgt ccacgacctg gccgttcttg acgaacggtg taaagcgttc gcccaggcca
     1921 tatatgtatt cgccgacgcc aagatcgagc tgttcgcgca tataggcgcc ttcgccggcc
     1981 actgtcaggt accctgtatt cctctggccg tttcccgtta tcctctttcc gtcgtataaa
     2041 aagtccatgc tccagccgcc tgtcttcctg agcctcatgg caagcctgcc tgaagtcatg
     2101 gtgatatatt catcatcctg cgttatcaca ggtgaatagc cttctttcct gtatagttcg
     2161 aatttgaggc ttttgggcag cccacccttg aagtggtata ccttcacgcc gattacatcc
     2221 tcaaacggcg agaaaaactc atatgtcagc atgggaccgt cgagcgtatc ccccctgtgc
     2281 cttaccggtc ttgtggcgac aaacagcttc aatatgccgt cacagatcct ggtatccctg
     2341 atctccacag gagatgccaa agtaacgccg tccctcagca gccagtaacc attggaaaat
     2401 ttcataccga tacctcctgt aaacgcagaa atattactat gtccgggtcg atctgcagaa
     2461 tgttgcagca aatcgattgt cataatataa ttttattgat atccatgcgc aaattcttac
     2521 tttattttga taatttctat tacttttttg atacaggcgg tttatatgga taaattactg
     2581 ctgaaagaag acagggtcca cggcgacgtg tcattcccgc tgggttcata ttatatggaa
     2641 ataccgtccg gctccgtagt cctggactgc cactggcacg aggaaatgga gcttctgctg
     2701 gtaacttcgg gcaaagcgca gttccggata gaggccgaat attatgaagt ccatcaaggc
     2761 gagtgtattt tcgtcaacag cggagaactg cacgccgggt tccagatcga caaatcccca
     2821 tgcagctaca aggctctggt tttcagccct tcgctgcttt acagcagttc cttcgacctg
     2881 accaggacca actatgtgga tccgatcatg aaaaactcgc tcatcgtcaa aaggcatata
     2941 accgcatctc cgggctggca gtccgaactt ctgggcaggc tggagatgct gctgcgatat
     3001 ttggatgcca ggcctcccgc atatgaactg ctggtcaagt cggaactctt cggcatattc
     3061 gcacttctgt cggccaactg tatatcttcg gctaataccc ctgaacgcag ccaggaccat
     3121 ttcaggctgg aaaagctgaa gacggcgctg aaatacatac aggacaatta ctcaaaaaag
     3181 ataagcactt tcgacatagc cgccagtgtc gacatgagcg aaggccattt ctgcaggtta
     3241 ttcaagcatt atgtgaagaa gacccccgtc gagtacctga actattacag gatcacaagg
     3301 gcggcaaggc tcctcgagga aacgggtatg aaagtgctgg aggtggccat ggaagtcggt
     3361 ttcgacaatc tcagctactt catagggacc ttcaaacgcc acatgggcat gactccctcg
     3421 aagtacagga gcctcagggg caaggcaatg ttatgatacg cgaaaataaa aaaggggctg
     3481 tccgacagct tcggacaagc ccttttatgt tgtcatcatc ccagtgatac ggtcaactgt
     3541 tcgggggatc cctgcggcct cacgaagcgt gatatcccat gggctcacag gtcaaagccc
     3601 ccggcagcat gaaagtccgt ccggctccaa agttcacccg agttcaacta tcacctgcga
     3661 cttgctgccc gccggctgta ccggtatcct gtcgacctct ttcccgttca gtattatctt
     3721 ttttactccg cagcacacat gggacggatt cctgacctcg atctcgtact cagccccgcg
     3781 gaatatcctc ctggccctga agccatccca tgacgacggg atgcacggat cgaccgtgag
     3841 gcagtcatag cccggccgga tgccgagtat gtactggctg gacgcgacca tgttccacgc
     3901 ggcggtaccg ctcagccatg aattcctgcc tataccgaac tgcggatgtt ctttgcccag
     3961 tatattttgc gcgtaaacat acggttcgac ctcgtaatga tccgcatcct cattgcgcct
     4021 ggccggaagt atctggttgt agtattcaaa tgccctgtcg ccgttgccct ccattatctc
     4081 ggctatcatg acccatgggt tggtatggca gaatatgcct ccgttctcct tcgctcccgg
     4141 aggatatgtg gtcacgccgc ccttggtggg atcgtgctgc gtatatgagg gatacattgc
     4201 cacgatgccg tacttcgtat tgagatattt gttgaccgac tccaggcaaa gacgtgcgcg
     4261 ttctttatct gcaacgccgc caagtattgc ccatgactgc gagttgatga atatcctgcc
     4321 gaatgtgttt tcctttgagc ccagcggctt gctttcatcg tcgaatgccc tcacatacca
     4381 ctcgccgtcc cagcaggtgt cattgatggc ttttttcatc ttttcggaca tgtctctgaa
     4441 ccttacggca tcctcattcc tgcggaggaa aaccgcaagc tcttccattt cgtttgccgc
     4501 cctgcagaag agcatcgatg tgaagacgct ttcggcaacg cccttgccgg tatccaggtt
     4561 cagcgtatcg ttccagtcgg cacggcccgc cagggctata ttgtgagggc ccttccatgt
     4621 atcggtgaat tccagcgctt tgaccaggtg ttcccagact gttccttcgg tcttcttgtc
     4681 attgtaaagc actttctgat ccaggaacgc aaaatcgccc gtctccttca cgtatgcatt
     4741 gacggcgagg atcagccaca gatggtcatc ggagtaccag tcgaacttct tgatgggagc
     4801 gtctccaact ccgccctctc ctgtcagcgg gaagaaaagg tgataagcgc gcccgtcggt
     4861 atactggcac tgcaggagct ttatgatcag gcccttcgcc tcttcgggga tggtatgcat
     4921 gacgccgagc gtatcctgtg cgctgtccct gatgcccatt cctcttccga ggccaagctg
     4981 gtacatcgat acgaacctgg accagttgaa ggtagtcttg cactgatact ggttccacat
     5041 attcacaaac aggttcatct cgcggtcagg cgtatcgaca tgcagtttgc ttatgtaatc
     5101 cttccagtat tcgttaaggc gtccgagtgc cgccttcgct tcggcgggat ccaggtaagg
     5161 ctgtatttcc ttctttatcc cgtccctgtt gtcggtatat cccaggatga aaacgatctc
     5221 cttcgtttct ccgcggccaa gttctatctc attgcagaat gctccaacgc cgttggtcct
     5281 gcgggaaatg gaattggagc atgccccctg ctcgacggct atcgggttcg attcggaacg
     5341 gtatctgccg atgaaagtct cgagattgca gtcatagctg gaaacttcag ccccggttgc
     5401 gaagaacgat gcgctaccgc ttacaaaatg cggataccag gtgattatgc cgtcattata
     5461 gcggcactgg ttgatctgct gcacccagtc cacattctgc tggtccatta cagcatccca
     5521 gaagcagaac tcggcgtagc tgaagatctg gagctttttc ggcacattca gcgtgttcct
     5581 gaccttaagg agccatatct cgaaatccct gtcatccggc acgaaataag tcaggcttgc
     5641 cgttatcccc ctgtattctc cctcaagcac cgtatacccc atgccatgcc tgcacaggta
     5701 gctgtcaagc ttcgtacacg agggctggta gccgggattc cagtattcac cggtggcagc
     5761 gtccctgata tatatgtatc tgccgggcct gtccataggt atgctgttgt agcggtacct
     5821 cgttatcctc ctgttctggg gatccttgtg gaaactgtat cctccgcccg tctgcgtgac
     5881 gatgcccccg tacttcccgc tcccgatata gttgatccat ggggtcggag tatcgggtct
     5941 ggtaatgacg tattcatttg cggcctcatc aaaatagccg aacttcatgg ctgcctcgac
     6001 ctcccttgtt ttctttgtct gatgcgtgat ctggtataca gtaattataa cgcatcaggg
     6061 aaaaatttgc gattacggaa atttttctgc ctctgcgcgt ttcgacgcgg aatacctgtt
     6121 atcaccccgt gctgtaattg aaaaatgtaa aataatccta tataatagaa atgtcggaag
     6181 ccttgacaga gtggcatttc ggcctgatac catccacatg gggagatgtt gtccatgagc
     6241 gcatatgtca atataagcaa agaattgcgg aacttcctcg gtaccaacaa gatattcagg
     6301 tacatcctgc cgctggatgt agtgataatg tttgcaggcc taatcctcat tttccttgac
     6361 gacacggtag gcgtcaacat tggagggttc ctgagagcgc tggtgcactg gacctttata
     6421 acagggctgc ttctgacata tgccaatatg aaggaacgtc ctttatatat aggattgatc
     6481 gggtatggcg ccatacacct gataagcttt ctgagacttc tgttcaaata tggatacttc
     6541 gattgttccg accttttcgg atccgccgta tacgccggac tgggatacct ggtcctgagg
     6601 aaggccctgg ccggttcagc cgataaaact ccaaacacag ccggttgaat cctgctgaca
     6661 gtccgctttg ccacagacag cttacggacg ggtcgagcgc gtttgcccga cccgttttta
     6721 ttttccgtac ttttcatcct catttccata aaagtcgatg gtatatccgg gtaaaacacc
     6781 ggtcccggca catggccggc ttcgtatcct tcacgtccgg accataaacg gcctgtccgc
     6841 ttacaaatat tttcaaaata tatattgaca tataatatta tacattgtat agtattatgc
     6901 gtaaggtaat atgaaaggca gtgaacagca tgaaatccag ggtatttatc acaggagcga
     6961 caggcggact cgggaaagcc tttgcagtgg aatgcgcaag ccggggctcc gacctttttc
     7021 ttacggacat ctcggatgct tcgctggcaa agctctctga gagcctggcg aacacatacg
     7081 gcataaaagt atccttttac agctgcgacc tgacagacgc cggttcaagg gcaggcctct
     7141 atggtcacat caggtcccgc tgcttaatgt tcgattttct cataaacgtc gccgggctcg
     7201 atttcgaagg agcatttgca gaaaggaccc ccgaccagat caggacgatg gtccgtctca
     7261 atatcgaagc caaccttgaa ttgacccatg aactgcttaa actgcgcgat cctgcaagga
     7321 cgttcaggat aatcaatgtt tcaagcctgg cagcattcta tccgatgcct gtgaaggcca
     7381 tgtattctgc ctcaaagcgg ttcctcctca acttttcgag ggcgctcggc gaggaactgc
     7441 gttcacgcaa tgtcaccgta acggcgctgt gccccgccgg catgcctacc acgcccgagt
     7501 gcatcgaggc gatagaagcg cagggtctcg ccggaaggat aaccacctgt aatgtcggct
     7561 acgtggcatc aaaaacgatc gatatggcgt tgagaggaaa acaggtgtat ataccgggac
     7621 tgctgaacag gatactcagg gcggcaggtt ctgtcgtacc ggattccatc atcgcacgcg
     7681 cagtgggctc tcgctggaat gcggcacaca caaggcgcat gcggcggcat gggactaata
     7741 aggcttatac ctgaaaggag ttgtgcatgg aatggaaaat tcgaacgata cgattaaaac
     7801 gatagtgatc acaggcgcca catccggcat tggactcgca gcggctaagg aactggcaat
     7861 gaaaggcgca tatgtgatcg gtacgggccg ttcggaagaa cggtgcaggt acgcggagca
     7921 ggaggtcaaa agcgtctgcc cggacgcgaa tataacatat ctggctgccg atctctcatc
     7981 cctttcagaa gtcaggcgtc tggcgtccga catacgcgag acacttgcaa aagacggttt
     8041 ttcacaactg gacgtactta taaacaatgc cgggacagta tcaagctggt atatatcgac
     8101 tgttgacggc tttgaactgc agttcgcggt gaaccatctg gcgcctttcc tgctgacgaa
     8161 tgaacttctt ccgttgcttg gacggtcccg ggcgggaacg gtcatatgta ccagttcagg
     8221 ctcgcactat agaaccaggc tgcggtggaa tgatataatg ttgaggaagc attacaatac
     8281 acttctggca tataagcaga ccaagctggc caatgtgctt ttcgtgaccg aactgaacag
     8341 gaggcttgcg gcgtccggca gcaatataaa ggcctttgcg ctggacccgg gactggtgga
     8401 taccgatatc ggcttcaaag gcacatccgg aatagccaaa ctggtatgga agttccgcag
     8461 taaaggcggc acatccccgg aagtaccggc gcattccatg gcttatctcg ctctggaccc
     8521 tgaaccgagg tccacgggaa aagtctactg gaaagactgc aggccgaaag agccaagccg
     8581 ctactcgcaa aagccggatg ttgcaaaaaa gctgtgggag ctgtcagaaa ggatgtgcgg
     8641 gatcattggc cataaaagca gccaggaaca gaagcaaact gctgtctgaa caaaggaggt
     8701 agttttattg gacgcccaga tgaaaaaggg gctgctggac gcatgcgtcc tccacatcct
     8761 gaccaaaggc gacacctatg gttataagct taccagggag ataacagacc tgatggatgt
     8821 gtcagagtcc tcgctttacc cggtcctcag gcgccttgag acccagggct tcctcgagac
     8881 ctattccgcc gagcattcag gccgcctgag gcgatattac aggataac
//
LOCUS       DAOSCC010000094         8926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5126_length_8926_cov_4.414384, whole genome shotgun sequence.
ACCESSION   DAOSCC010000094 DAOSCC010000000
VERSION     DAOSCC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_8926_cov_4.414384"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLG72_06355"
     CDS             complement(<1..844)
                     /locus_tag="PLG72_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOJ80454.1"
                     /translation="MPITEFLSRNAALYGNEICLTEINLDLQEKHNVIWREYELIENN
                     PAGEYRRDMTWRVFDEKANRLANLLIKRGVKKGEKVAILLMNCLEWLPIYFGILKAGA
                     IAVPLNFRYTEEEIRYCLDLSDSVALIFGPEFIGRLENIYDRIPKVRMLFYAGEGRPS
                     FAESYDRLTANCSSEAPNVGITDDDDAAIYFSSGTTGFPKAILHTHRSLVSACITEQK
                     HHGQTREDKFLCIPPLYHTGAKMHWFGSLLAGSPAVILRGIKPDWILRTVSEEKITIV
                     WLLVP"
     gene            complement(1090..1788)
                     /locus_tag="PLG72_06360"
     CDS             complement(1090..1788)
                     /locus_tag="PLG72_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80455.1"
                     /translation="MLKKVLAAVLAICMMFAALTACGIKKKDDGAGIAASGPKKNSSE
                     KDAAAAKKEIDDALDTLEEAGVELDDDLQEMLENIDEADIEAAQAARATDVKISVDAP
                     EGWTVENNDYANYSAVNGMRTFMVMCSEEPSGVSDIAAYAKNNQDKMKTVFSEAEYSD
                     ITSVNIDGTKGGRFHIDLEIFGLKQRQIYVYYCKDGLVTVVQGCYIMGMGDDEAESEA
                     EINKMIDSVRVEKR"
     gene            complement(2010..2684)
                     /locus_tag="PLG72_06365"
     CDS             complement(2010..2684)
                     /locus_tag="PLG72_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOJ80456.1"
                     /translation="MGFEGFSAEALKFLLENRMNNSKEWFEAHRQEYRKYVYEPFVEL
                     VKDLAPAMKEIDDGIITVPSKIISRVRRDTRFTKDKTLYRDNAWIVFLRDKTQMSVSP
                     CFWFELGQNGTSYGVGYYKAENASMARMREMILERHPSFMAALRAYESQDEFVIGGES
                     YKKCKYPEQPENIKQWLIRKNIFFESVHRDFGPAFSAGLPDMLINGFRKLKPIYDFFL
                     LVKSSV"
     gene            3029..5698
                     /gene="adhE"
                     /locus_tag="PLG72_06370"
                     /gene_synonym="adhC"
     CDS             3029..5698
                     /gene="adhE"
                     /locus_tag="PLG72_06370"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HOJ80457.1"
                     /translation="MAKTRKYEVIDSVEKLDAALARVREAQKIYAGYTQEQVDRIFFE
                     AAMAANKERIRLAKMAVEETGMGVVEDKVIKNHYASEYIYNTYKNVKTCGVIEEDKAY
                     GVKKIAEPVGILAAVIPTTNPTSTAIFKTLIALKTRNGIVISPHPRAKKCTVEASRIV
                     YEAAVKAGAPEGIIAWIDVPTLEMTNHIMKQADLILATGGPGMVTAAYSSGKPAIGVG
                     PGNTPAIIDSSADILLAVNSIIHSKTFDNGMICASEQSVIVLDDIYDKVKAEFERRGC
                     YFLDDGEKQKVRETIIINGSLNAKIVGQTASAIASLSGFSVPEGTKVLIGEVEKVDLS
                     EEFAHEKLSPVLAMYRAKTFKEAMDKAERLIADGGFGHTASVYLDTIRAKDRLNEFTE
                     RMKTCRILVNTPSSHGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVGVKHLLNIKTVA
                     ERRENMLWFRTPEKVYMKKGCLPVALDELKNVLGRKKAFIVTDRFLYGNGFTKHVTDR
                     LDEMGIVHTTFYNVSPDPTLACAKEGTEAMKSFGPDCIIAIGGGSAMDAAKIMWVLYE
                     HPEVDFMDMAMRFMDIRKRIYTFPKMGEKAYFVAIPTTSGTGSEVTPFAVITDETTGV
                     KYPLADYELLPDMAIVDADLMMDQPKGLTAASGIDALTHALEAYASMMATDYTDGLAL
                     RAVRMIFEYLPRAYEYGAADPKAREKMADASTIAGMAFANAFLGVCHSMAHKLGAFHH
                     LPHGIANALLISEVIRYNAAEAPVKMGTFPQYDHPHTLERYAEIADSLGLNGRNNEEK
                     LESLIRAIEELKEKVGIKKSIRDYGITEEDFLGSLDEMAEQAFDDQCTGTNPRYPLIS
                     EIKQMYLKAYYGSEQNAAGHASEKAEEPKTIPA"
     gene            5882..6631
                     /locus_tag="PLG72_06375"
     CDS             5882..6631
                     /locus_tag="PLG72_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ80458.1"
                     /translation="MKLDNVIAVRKTKVVYRDGDLAVKVFDENYSKADVLNEALNQAR
                     IEQTDLHIPEIVEVTKIDGKWAIITRFIEGKTLEALMNEEPEKYDKYLEFFVDLHLEV
                     HRQQSPLLNKLKDKMHRQISMTDLDATTRYDLHTRLESMPNHKKVCHGDFNPSNIIIA
                     KDGTPYILDWSHAAQGNASADAARTYLLFWLAGSTETAKKYLRLFCRKSDTALQYVQK
                     WIPLVAACQSIKGDPNERSLLLRWVDVIDYE"
     gene            complement(6749..7423)
                     /locus_tag="PLG72_06380"
     CDS             complement(6749..7423)
                     /locus_tag="PLG72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ80459.1"
                     /translation="MGNGNHDIRGEVMANRIIINGSQSDGIQDRTGTRTGQGNRSNGI
                     GSGQSVQSLQNAVPEKDRQQTEEFPLDENVWGNDRALASKLDNEVLAERRLFVRVRHV
                     QKLTCDTIYEEIDKEPVKLASPLQFMSVDLSAAGIGIVCENEIEPGKILGIRMLLDRI
                     PYDIKCRVVYCIPFDGKFRAGLKIAEKDKRFMRHLKIYVARITLTNEYASDSGGSQLF
                     SQAKRV"
     gene            8382..>8926
                     /locus_tag="PLG72_06385"
     CDS             8382..>8926
                     /locus_tag="PLG72_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOJ80460.1"
                     /translation="MGEIHSFEDEKLIVCVMYSDPAFLEKAEKMLVDTFGPYDLVSGD
                     YSFSDNFSAYYDEELGGKAIRRIYSFERCIDPSRLASIKTLTNSFEETLSEGGKRRIN
                     LDPGLLSHGRVVLASTKNSGFRIPLAGGIYAEITLFYGQGRWNRLPWTYVDFQSEPVK
                     DFLMEVRRIYLGQRKAWLKGKS"
BASE COUNT         2295 a         2348 c         2132 g         2151 t
ORIGIN      
        1 atggtactag cagccacacg atggtgatct tctcctcgga aacggtccgc aggatccagt
       61 ccggcttgat acctctcagg atcacggcag ggcttcctgc cagcagactt ccgaaccagt
      121 gcattttggc tcccgtgtgg tacagcggcg gtatgcagag gaacttgtct tcccgggtct
      181 gcccatggtg cttctgctcg gttatgcacg ctgaaacaag gctcctgtgg gtatgcagta
      241 ttgcctttgg aaaaccggtc gttcccgatg agaaatatat cgctgcgtca tcatcgtccg
      301 ttatcccgac attgggagct tccgaactgc agttggccgt gagacggtcg taactttccg
      361 cgaacgacgg gcggccttct cctgcataaa agagcatcct cactttcggt atccggtcat
      421 atatgttctc aagcctgcct atgaactcag gtccgaaaat caaagcaaca gaatcggaca
      481 gatcgagaca gtaccgtatt tcctcttcag tatatcggaa attgagcggt accgcgatgg
      541 ctcctgcttt aaggatgcca aagtagatgg gcagccattc caggcagttc atcagcagta
      601 tcgctacctt ctcgcccttc ttcactccgc gcttgatcag caggtttgcc aggcggttgg
      661 ccttttcgtc gaatacgcgc catgtcatgt cgcggcggta ttcgcccgca gggttgtttt
      721 caataagttc atattcccgc catattacat tgtgtttttc ctgcagatcg agattgattt
      781 ccgtaagaca gatctcattg ccgtacaaag cggcatttct tgacaagaac tcagttatag
      841 gcatgactca tcctcccgtc ctgctggtaa gaatcaaatg aaatgcagtg aaaaccttac
      901 cgtgcttcat aactgcaaaa catcacgtaa attatatgac aataccggca tttaatcaac
      961 agtttgttgg aaggaaagcc agcctataac tgtacatgta cgacttatat gtatataaac
     1021 ttacttaaaa cagttatgct ttccgaaata gaatcttttg ccgaatcaca tcggtttttt
     1081 ccggacgcct tacctttttt ccactctgac tgaatctatc atcttattta tctcagcttc
     1141 agactcagcc tcatcatcgc ccatacccat tatatagcag ccctgtacca cggtcaccag
     1201 gccgtccttg cagtaatata catatatttg cctctgtttc aggccgaata tctccaggtc
     1261 gatatggaag cgtccaccct tcgtaccgtc tatattgacg cttgttatat cagagtactc
     1321 tgcttcagaa aagactgttt tcatcttatc ctggttattt ttggcatatg cagcgatatc
     1381 gctgaccccg gacggttctt cagaacacat taccataaaa gtgcgcatgc catttacggc
     1441 actgtaattc gcatagtcgt tgttttccac tgtccagccc tccggcgcat cgaccgatat
     1501 ctttacatca gttgctcttg ctgcctgggc agcttcaata tctgcttcat cgatattctc
     1561 cagcatttcc tgtaaatcat cgtcgagttc cacaccagct tcctccagtg tgtccagtgc
     1621 atcatcgatt tctttctttg ccgcggcagc atctttctcc gaactgtttt tcttcggccc
     1681 tgaagcggcg atgcctgccc catcgtcctt cttttttatg ccgcacgctg tcagtgcggc
     1741 aaacatcatg cagattgcca gtactgctgc caatactttt ttcaacatcg tgatccccct
     1801 aaccttttat ttttccggcg gcacatccgt catccggatg ccgggcacaa gcttctgcct
     1861 gtcctgtttt gccgagtgtt tttgtttgca agtctattct attgcaaagc attacaggat
     1921 gatatcaccc gggaggggtg aatcaccatg aaaaaggatg attttgaagg gttaaggggt
     1981 gattggcacc tgcctgggca aacgatgtat cacacactgc ttttcacaag cagaaagaaa
     2041 tcgtatatcg gcttcagttt tctgaagcca tttatgagca tatccggcaa tcctgcggaa
     2101 aatgcaggcc cgaagtccct gtgtacgctc tcgaaaaaga tatttttcct gatgagccat
     2161 tgctttatgt tttcaggctg ctcaggatat ttgcactttt tatatgattc tccgcctatg
     2221 acgaactcat cctggctttc ataagctctc aatgcagcca tgaacgacgg atgcctttcc
     2281 agtatcatct ccctcatcct cgccatagac gcattttcag ctttataata cccgactccg
     2341 taactggttc cgttctgccc caattcgaac caaaaacagg gcgacacaga catctgcgtc
     2401 ttgtccctca ggaagacgat ccaggcgttg tccctgtaaa gggtcttgtc cttcgtgaac
     2461 ctggtgtccc ttctcactct cgatatgatc ttcgacggaa cggtgatgat accgtcgtct
     2521 atttccttca tggcaggcgc cagatccttt acaagttcga caaaaggttc gtacacatat
     2581 ttgcggtact cttgcctgtg cgcctcaaac cattccttgc tgttgttcat cctgttttcg
     2641 agaagaaact tcagagcctc cgctgaaaat ccttcaaacc ccatggctga tcctccggtt
     2701 tcgtatgata cgcgctgcat ggccctggct gccgtactga atatacaata tgattttaag
     2761 atacgcatgt caagttatac attcaaagca attcatcatc ctgccggacg gagatcgcat
     2821 gcgtgtgctt aaggttttgc ggccaattca caaattttat aaaaaaatca gccaataatg
     2881 cccgaaaatt atatgttaag taattgacaa aaaatcatgt gcgtgttaat attttatcaa
     2941 agatagttaa atatttatca aagttagtta taaaaataac ataacagcaa aagatgatcc
     3001 ggacagcaca tgtaaaggag gttttaatat ggctaagaca aggaaatatg aagtcatcga
     3061 cagcgtggag aagctggacg cagcgcttgc ccgtgtaaga gaagcgcaga agatatacgc
     3121 gggttatacg caggaacagg tagacaggat cttttttgaa gcagccatgg ccgccaacaa
     3181 ggaacgcatc cggcttgcaa aaatggcagt ggaagagacg ggtatgggcg tcgtggaaga
     3241 taaggtgatc aagaaccact acgcgtcaga gtatatatac aacacataca aaaatgtgaa
     3301 gacatgcggc gtcatcgagg aagacaaggc gtacggcgtc aagaagatcg ccgagcccgt
     3361 aggtatcctg gcagccgtta tcccgaccac gaatcccaca tctactgcca tattcaagac
     3421 actcatcgcc ctgaaaacga ggaacgggat cgtaataagc ccgcatccga gggcaaagaa
     3481 gtgcacggtc gaggcttcaa ggatcgtata tgaggccgca gtcaaagccg gagcgcctga
     3541 agggatcatc gcctggatcg acgtgccgac gcttgaaatg acgaaccata tcatgaaaca
     3601 ggcggacctg atcctcgcaa ccggcggccc cggcatggtg acagccgcat attcgtccgg
     3661 caagccggcc atcggcgtcg ggcccggcaa cacacccgcc atcatcgaca gttctgcaga
     3721 catcctgctg gcggtcaact caatcatcca ttcaaagaca ttcgacaacg gcatgatctg
     3781 cgcatctgaa cagtccgtta tagtgctcga tgatatttat gacaaggtga aagccgaatt
     3841 cgaaagacgg ggatgctatt tcctggatga cggggaaaag cagaaagtac gtgagacgat
     3901 cataataaac ggctcgctga atgcaaagat cgtcggccag acggcttccg ccatagcatc
     3961 gctttcaggc ttcagcgtac ccgagggcac gaaggtgctg atcggagaag tcgaaaaagt
     4021 cgacctttcc gaggagttcg cacatgagaa gctctccccc gttctcgcaa tgtacagggc
     4081 aaagacattc aaagaagcta tggataaggc tgaaaggctg atcgccgacg ggggcttcgg
     4141 ccacaccgct tcagtgtacc tcgacaccat ccgtgcgaaa gacaggctca atgaattcac
     4201 cgaaaggatg aaaacctgcc gcatactcgt gaacacacca tcatcccacg gcgggatcgg
     4261 cgacctctac aatttcaaac ttgcgccgtc gctcacgctg ggatgcggct catggggcgg
     4321 caactcggtt tcggaaaacg tcggcgtgaa gcacctgctc aatataaaaa cggtggcgga
     4381 aaggagagaa aatatgcttt ggttccggac ccctgaaaag gtttatatga aaaaagggtg
     4441 cctgccggtg gccctcgatg aactgaaaaa cgtgctcggc aggaaaaaag cattcatagt
     4501 cacggacagg ttcctttacg gcaacggttt cacgaagcat gtcacggaca ggctcgacga
     4561 gatgggcata gtccatacca cgttctacaa cgtatctccc gatccgacac tggcatgtgc
     4621 aaaggaaggc accgaagcca tgaagtcatt cggtcccgac tgcatcatcg ccatcggtgg
     4681 cggctcggca atggacgccg ccaagataat gtgggtgctc tacgaacatc ccgaagtcga
     4741 cttcatggat atggcgatga ggttcatgga catcaggaag cgcatataca cattccccaa
     4801 aatgggcgaa aaagcatact tcgtggcaat accgacaaca tccggtaccg gaagcgaggt
     4861 aacgccgttt gcagtcatca cagatgaaac cacaggtgta aaatacccgc tggccgatta
     4921 cgaactgctg ccggatatgg cgatcgtgga cgcggacctg atgatggacc agcccaaagg
     4981 cctcaccgcc gcttcaggca tcgacgcgct cacacatgcg cttgaggcat acgcatccat
     5041 gatggctact gattacaccg acggacttgc gttaagagct gtcaggatga tattcgaata
     5101 ccttcccagg gcatatgaat acggcgccgc cgacccgaaa gccagggaga aaatggccga
     5161 tgcatccaca atcgcgggta tggcattcgc gaacgcattc ctcggcgtct gccattcgat
     5221 ggcacacaag ctcggagcgt tccaccacct gccccacggc attgccaacg cactgctgat
     5281 cagcgaagtc atccggtaca atgctgccga ggcgcccgtc aaaatgggca ctttcccgca
     5341 gtatgaccat ccgcacacgc tggagaggta tgccgagata gcagattcac tcggcctgaa
     5401 tggcaggaac aatgaagaaa aacttgagag cctgatcagg gccatcgagg aactgaagga
     5461 aaaagtaggc atcaaaaaga gcatcaggga ttacggcata acggaggaag atttcctcgg
     5521 aagcctcgac gagatggcgg aacaggcttt cgacgaccag tgcaccggca ccaatccgag
     5581 gtatccgctg ataagcgaga taaagcaaat gtacctgaaa gcatactatg gaagcgaaca
     5641 gaatgccgca gggcatgcat cggaaaaggc tgaagaaccg aagaccatac cggcgtaagc
     5701 cgtatcagat atactgaccc gggcgggggc ggccccaccc gggccgggct ttgagaaaac
     5761 cgcaacggct ttggaacagg cacgcaaacg cataacctgc atgatctgct gcggaataca
     5821 cggcgtgtat gcatcgtttt tgcttgcacg caagacctgt accgggaagg aggccgaaga
     5881 tatgaaactg gataatgtga tcgcagtacg gaaaaccaaa gtcgtttacc gcgacggcga
     5941 ccttgccgta aaggtattcg acgaaaacta ctcgaaggcc gacgtgctga acgaagcgct
     6001 caaccaggcc aggatcgagc agacggatct ccatatccct gaaatagtcg aggtcacgaa
     6061 aatcgacggg aaatgggcca taataaccag attcatagaa gggaaaaccc tggaagccct
     6121 tatgaacgag gaaccggaga agtatgacaa atacctggaa ttcttcgtcg atctgcacct
     6181 tgaagtgcac cgccagcagt ctcccctgct gaataagctg aaagacaaga tgcaccggca
     6241 gatatcgatg acggacttgg atgcgacgac ccgctacgat ctccacacgc gcctcgaatc
     6301 tatgccgaac cacaaaaagg tctgccacgg cgacttcaat cccagcaata tcataatcgc
     6361 aaaggacgga acgccgtata tactggactg gtcccatgcc gcgcagggga acgcgtctgc
     6421 cgacgccgca aggacatacc tccttttctg gctcgccggc agtacggaaa ccgcaaagaa
     6481 gtatctcaga ctgttctgca gaaagagcga cactgcattg caatatgtcc agaaatggat
     6541 ccccctggtg gcagcctgcc agtcgataaa gggcgatccc aatgaacgca gcctgctgct
     6601 tcgctgggtg gatgtcatcg attatgaata gtgccgcaga acaaggatat ggcaaaaccg
     6661 ataaaacaaa acggatcttg cgtccgtacc gggggctgta ttcggtgcgg accgcaagat
     6721 ccgtgtgatc cgggtccagg ctggcgggtc atacccgctt cgcctggcta aacagttgtg
     6781 agccgccgct atcggaagcg tattcgttgg tcaatgtaat gcgcgccaca tagattttta
     6841 agtgtctcat gaagcgcttg tccttctcgg ctattttcaa cccggctctg aatttcccgt
     6901 caaatggtat gcaatatacc accctgcact ttatatcgta cggtatcctg tcgagtaaca
     6961 tcctgatgcc aagtatcttg ccgggttcga tctcattttc acagactatg ccgatgccgg
     7021 cagcggaaag gtccaccgac atgaactgga gcgggctggc cagtttgaca ggctctttat
     7081 ctatttcttc atatatggta tcacaggtga gtttctgtac atgcctgacc cttacaaaaa
     7141 gtctcctctc tgccagcact tcattatcga gcttgctggc cagggccctg tcattccccc
     7201 agacattttc atccagcgga aattcttcag tttgctgcct gtctttttcg ggaacagcat
     7261 tttgaaggga ctgtaccgat tggccgctgc caataccatt ggatctgttt ccctgtccag
     7321 tcctggttcc ggtcctgtcc tgaattccgt cgctctgact tccgtttatt atgatcctgt
     7381 tggccataac ttcgcccctt atatcatgat taccattccc catatatatc ataacaccgt
     7441 ccgcgttata taaaaatagt tgatgcgtcc taagatatgg gaccctggta ccatttttga
     7501 actatcccca ctgccctgta ctttacacgc aacgcttgat acgctgtatt tatacaccat
     7561 gtttacatgc tgtactccat actacactcc cgcggccttc cttttctgct cctggtgtgc
     7621 tgccttaata cctgcccgga caagtgaagc gttatctcat cggggcatct ttccttccgg
     7681 aacagtattt ggtcactcat ttcccttttc gcattgccct cccttgcagc agggactcaa
     7741 gtgtacaaaa ttctaattgt tgtgagacgt cctggctttt tattccatta ttttattaca
     7801 ttatatcgtg tattattgcg ttatgtaaat tacatattgt aaattgtcaa tatcaatgat
     7861 gcctttgcac cacaacaatt caatttttgt acacccaggt cactatttcc cataatattt
     7921 cccgatacct ggcccaggac agccctatac cggatccaga atgtccgggc gccggatccc
     7981 ttacctgaag taaaactgca agcaaagcag ctgctatacc ggatccagaa tgtccgggcg
     8041 ccggatccgg tacatcacag caccggattt caatacatcc ttgcactgta tttcaacaca
     8101 tcccggcatc atatccaatg cgtcccgctg ccggatccaa agtcagccac gcattccagg
     8161 tacatgtact tattatcctc gggccgggcc ggtttcataa tcggccaggg tgtttaagtg
     8221 aaccggccat tgacgataca tttatccggc atggcaaaaa tgctataatg cacataggga
     8281 caggctgact gttccgctgc cgtcctgtgc cggccagtcg gttttttatg cggtttgcgt
     8341 tccggccggc agcaacactc aactttcgga gggatctacg catgggcgag atacacagct
     8401 ttgaagacga aaaactgata gtctgcgtga tgtacagcga tccggccttc ctggaaaagg
     8461 cggaaaaaat gctggtagat acgttcgggc catatgacct ggtttccggg gattattcct
     8521 tttcggacaa tttctcggca tactacgatg aagagctagg agggaaagcc atcaggagga
     8581 tctacagctt tgaacggtgc atcgaccctt cccgcctggc ctccatcaaa acgctgacca
     8641 attcgttcga ggaaacgctc agtgaaggcg gaaaacgcag gatcaatctg gatcccgggc
     8701 ttctcagcca cggaagggtg gtcctggctt cgacgaaaaa ctcaggtttc aggataccgc
     8761 tggcaggcgg catctatgcg gagatcacgc tgttttacgg ccagggacgc tggaacaggc
     8821 tcccatggac atacgtggac tttcagagtg agcctgtcaa agattttctc atggaagtac
     8881 gcagaatata cctgggacag cgaaaagcct ggcttaaagg caaatc
//
LOCUS       DAOSCC010000095         8763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5246_length_8763_cov_4.444074, whole genome shotgun sequence.
ACCESSION   DAOSCC010000095 DAOSCC010000000
VERSION     DAOSCC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5246_length_8763_cov_4.444074"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLG72_06390"
     CDS             complement(<1..300)
                     /locus_tag="PLG72_06390"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOJ80461.1"
                     /translation="MNIALIAHDNKKELMVDLCIAYKTILQKHTLYSTGTTASFISEG
                     AGLPVYSVSPGVLGGTQQIAARVSLNEIDMVVFLRDPVEGRGNEAEMYSLLRLCDM"
     gene            complement(314..598)
                     /gene="minE"
                     /locus_tag="PLG72_06395"
     CDS             complement(314..598)
                     /gene="minE"
                     /locus_tag="PLG72_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOJ80462.1"
                     /translation="MLLDLGKIFGKSKNSKDVAKERLKLVLIHDRANVSPQFLEMLKS
                     EIIKVITNYMDVDESALEIQLTRTKSDDGQSVVPALYANIPIKNIRKSGK"
     gene            complement(613..1413)
                     /gene="minD"
                     /locus_tag="PLG72_06400"
     CDS             complement(613..1413)
                     /gene="minD"
                     /locus_tag="PLG72_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOJ80463.1"
                     /translation="MSEVIVITSGKGGVGKTTSTANIGTGLALSGSKTVLIDTDIGLR
                     NLDVVMGLENRIVYDLVDVVEGNCRVKQALIKDKRFDGLYLIPAAQTRDKSAVKPSQM
                     IKLCEELREEFDYILIDCPAGIEQGFKNAIAAADRAIVVTTPEVSAVRDADRIIGLLE
                     ANEIRNPKLLINRLRADMVKRGDMMSIDDIIDILAIGLIGVIPDDEKIVVSTNRGEPA
                     ICDSKSLAGQAFRNVVRRIKGEEVPFMSFTEDDGFVMKLKKLFGLKAN"
     gene            complement(1544..2236)
                     /gene="minC"
                     /locus_tag="PLG72_06405"
     CDS             complement(1544..2236)
                     /gene="minC"
                     /locus_tag="PLG72_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOJ80464.1"
                     /translation="MEESIVSFKAKGNELILVMREEDDFGKIVEGIGRKLDSSGRFFK
                     GASLDVRYRGKKLDEEQERIISELIAEKASAEIISFKEDEEYQDQENSQDQEEPERAR
                     DKLKMKILYFRGLEEGMTKFYRGTVRSGTLVSYDGNVVVLGDVNPGGEVVAAGNVVVM
                     GSLRGMVHAGADGNKEAIVAALSLQPTQLRIADVITRPPDSSQPRQGFLPEIAFVKDD
                     MVYIETFLPQYK"
     gene            complement(2325..4451)
                     /gene="mrdA"
                     /locus_tag="PLG72_06410"
     CDS             complement(2325..4451)
                     /gene="mrdA"
                     /locus_tag="PLG72_06410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ80465.1"
                     /translation="MDKKLRNRYYILCLGFMVFGIMIVFRLFNLQIVEGEKYVADAQK
                     KILKEGEIEAPRGKILDRYGTPIAVNRQGYSLYIVKTNINTSEMNEMLLKLSDLLEKN
                     GDGHVNTLSKFLTYGPPVEFNGMTEKQIRDWQINADRLNMKEEDVKYNAYELFKYLRD
                     EKFKINTLFTDEQAYKIMVFRYELLISNWNFTTGGMVLLARDVSRETVAEIEERNHEF
                     PGIITMTEPVREYIDAYEEAHVLGYIRAISSKQYDELKDEGYDNDDLIGQTGVEKSAE
                     RYLRGKNGRVTFEVDADGNIIQKLVTMEEIPGNDVVLTIDTNLQKVAIESLARNIEKI
                     RNDKKGPAYNYGDANAGSAVVLDVHTGAVLTMVSYPSFDPSIYLAGPEDTEAQKMIIE
                     LTKDEQNLKALLNRTIQENYAPGSTFKPITAIAGLEKGVITPTSNTIVCRGTARIGGR
                     DLFCLEKPTYGHGPLSLKRGLETSCNLYFYELGTRTGIEALTYWANCFGLGKKTGIDL
                     PNEVSGMMSSIELKKKLRNDIWRPADTAQVSIGQFDNSFTPLQLANYTAALANGGKLY
                     RPHVIKEVIRYDGSVVNRTEPTYTEIPLKESTIAAVKEGMVAVTTAIDGTAQEAFKGL
                     PFEVAGKTGSAQTQSVGGRSPNGVFICYAPADDPEIAIAVVIERGVWGSNAAPVARDI
                     IDEYFGLKAADSRNTDLKPEKPLFNP"
     gene            complement(4483..4995)
                     /gene="mreD"
                     /locus_tag="PLG72_06415"
     CDS             complement(4483..4995)
                     /gene="mreD"
                     /locus_tag="PLG72_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOJ80466.1"
                     /translation="MKKKLLFYFVFIVFFLLLQTTVLQRAKILGVMPNLLITLTIVTA
                     LVRNSTEGAVVGLFSGLCIDMQFGAVLGFYGLLGMFTGIAAGSLSRKIYRENLLVVAF
                     FTFVYSVVYESVVFVINNIMSGDINFAFAFTRIILPEAGYNCAVSLLLFPLLVKAGKW
                     FDMSATARKY"
     gene            complement(4992..5852)
                     /gene="mreC"
                     /locus_tag="PLG72_06420"
     CDS             complement(4992..5852)
                     /gene="mreC"
                     /locus_tag="PLG72_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ80467.1"
                     /translation="MLRLFKSKWFIVSLVTIALLVVIGVSANKSSKLNWLSNILSVPL
                     KPVQGFFTSAGREIEDFFAYFQDIDELRKENEALKAEVEELRRQNREYSELKAKNDEL
                     RRALNLKAEFADYTITGANIIAVEPGNWFSVFKVDVGEQDGIRADFPVVTGSRGLVGR
                     VLDTDKSTSNIQTIIDEESAVSGWIAKPGGGHAIVKGDLELKQEGLCKLIYIPIEVDI
                     EVGDIVETSGVGGIYPKGIEIGKVIEVRKTNSELGRYAIIQPTADFRRLEEVFILKSE
                     KNDGTGSAGR"
     gene            complement(5869..6891)
                     /locus_tag="PLG72_06425"
     CDS             complement(5869..6891)
                     /locus_tag="PLG72_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ80468.1"
                     /translation="MGIFAKDIGIDLGTANTLVHVKGRGIVIREPSVVAINKNTREIL
                     AVGDDAKNMIGRTPGDIVAIRPMKDGVIADFDITQSMLKHFIRRAMGTGFISKPRVVI
                     CVPSGVTEVEKRAVEDATLAAGAREAYLIEEPMAAAIGANLPVEEPSGSMIVDIGGGT
                     SEVAVISLGGIVTSRSLRVAGDEFDESIVLFVKKEYNLMIGERTAEQIKINIGNAFLK
                     PKEEYMEVRGRDLITGLPKNVTLSSSEINEALKEPINSIIDAIKYTLEKTPPELAADI
                     MDKGIMLAGGGALLSGLDMLIKEETGMPVTIAENPLDCVAIGTGKVLEEIETLRSVLI
                     TPRKLR"
     gene            complement(6936..7634)
                     /gene="radC"
                     /locus_tag="PLG72_06430"
     CDS             complement(6936..7634)
                     /gene="radC"
                     /locus_tag="PLG72_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ80469.1"
                     /translation="MEYRLRMKDIPASERPYEKLEKYGPEFLSNAELLAVIIRTGSAE
                     ETSVALAQRLLSLGENKGGIAHLHDLSVEELRKVKGIGRVKAVQIKAVMELSKRISAS
                     FINNNKVTIKSPADVSMLLMEEMRHLKKEVFKVILLNTKNHIIKYLNVSVGSLNASIV
                     HPREVFSEAVRAGCSGMLLVHNHPSGDPEPSREDIETTHRLVNAGSILGIKVLDHIII
                     GDGRYISLKEQGLM"
     gene            complement(7734..8315)
                     /locus_tag="PLG72_06435"
     CDS             complement(7734..8315)
                     /locus_tag="PLG72_06435"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOJ80470.1"
                     /translation="MTKIILASASPRRRELLAQVGIPFEVVPGNIDEENADLSGTPEQ
                     KTEQLAYMKAMAVASKLESGLVLGADTIVVCDDEIFGKPRDEEDARRMLNKLKGREHK
                     VVTGICLVDASDGKARTDHEVTMVRFSEITDREIDAYISSGEPYGKAGAYAIQGRAAI
                     FVESLDGCYSNVVGLPLRRVYRLLKEFGVSACG"
BASE COUNT         1933 a         2466 c         1849 g         2515 t
ORIGIN      
        1 catgtcgcag agcctcagca gcgaatacat ttccgcctca ttgccccgcc cctcgaccgg
       61 atccctgagg aacaccacca tgtcgatctc gttgagcgat acccttgcag ctatctgctg
      121 cgtacctccg agcacaccgg gcgaaacact gtacaccggc agccccgctc cctccgaaat
      181 gaacgacgcg gtagtcccgg tggaatacag ggtatgtttc tgcaaaatgg tcttataggc
      241 tatgcacaga tctaccatca gttccttttt attgtcatgc gctatgagag caatattcat
      301 aactggctat accttatttc ccgctttttc ttatattctt tatcggtata ttggcgtaaa
      361 gcgccggcac cacactttgc ccgtcatcgc tttttgtcct cgtcagctgt atttccaaag
      421 cgctttcatc tacgtccata tagttggtga tgacctttat gatctcactc ttcagcatct
      481 cgagaaactg cggcgaaaca ttggcacggt catggatcag caccagtttg agcctttctt
      541 ttgcgacatc cttggaattc ttcgatttcc caaatatttt tcctaagtcg agcagcatcg
      601 tatacctccc cttcagttcg ctttcaggcc aaacaacttt ttgagcttca tcacgaaacc
      661 atcatcctca gtgaacgaca taaagggtac ttcttcgcct tttatccttc ttacgacatt
      721 cctgaaagcc tgtcctgcca gcgatttgct atcacatatt gcgggctcgc ctctgttggt
      781 cgacaccact attttttcgt catcggggat gactcctatg agccctatcg ccaggatgtc
      841 gattatatca tctatggaca tcatgtctcc tcttttgacc atgtcggcac gcagcctgtt
      901 gatcaggagt ttcggattcc ttatctcatt cgcctcaagc aaacctatga tcctgtccgc
      961 atccctgacg gcggatactt cgggggtcgt cactactatt gccctgtcgg cggccgctat
     1021 tgcatttttg aatccctgct ctatgccggc cggacaatct ataagaatat agtcgaattc
     1081 ctcgcgcagt tcttcacaaa gcttgatcat ctgcgacggc ttcacagctg acttgtccct
     1141 ggtctgagcg gcaggtatca gataaagccc atcaaagcgt ttgtccttga taagagcctg
     1201 cttgacgcga cagttgcctt cgactacatc cacaagatca taaacgatcc tgttttcgag
     1261 acccataact acatcaaggt tccttaaccc gatatcggta tctatcaaca ctgtcttgct
     1321 gcccgacaat gccagacccg ttcctatgtt tgccgtcgat gtggttttgc ccactccgcc
     1381 ttttcctgat gtgattacaa tgacttcgct catccaaata cctcctatgt gcctgtatgg
     1441 gcaatttgca gtgacgctga gattgttgag acctcgtaca tatgtaaaat atacagtttt
     1501 aaaagtaatt tgtcaattaa agcacttttt tgtaaataag caattacttg tattggggca
     1561 ggaaagtttc gatatatacc atgtcatcct ttacaaacgc tatttctggc aaaaagccct
     1621 gcctcggctg gctggaatcc ggcggcctgg taatgacatc ggctatgcgg agctgtgtgg
     1681 gctgcagcga caatgccgca actatggctt ccttgttccc gtcggcgccg gcgtggacca
     1741 ttccccgcaa agatcccatc actactacat tgcccgcggc gaccacttct ccgccgggat
     1801 tcacatcgcc cagcacaacg acgtttccgt cgtagcttac gagagtcccg gacctgacgg
     1861 tgcccctgta gaatttggtc attccttctt ccagtcccct gaaatacagg atcttcattt
     1921 tgagcttgtc ccttgcccgt tcaggctcct cttgatcctg gctgttttcc tggtcctggt
     1981 attcttcgtc ctctttaaag cttatgatct cagcggaagc tttctccgcg atcagttccg
     2041 atattatccg ttcctgttcc tcatcaagct ttttcccgcg atatctgaca tcaaggcttg
     2101 cgcccttgaa aaaccttccc gatgaatcaa gcttcctgcc tataccttcc actattttcc
     2161 cgaagtcgtc ttcttcccgc atcacaagga taagttcatt gcccttggct ttaaagctta
     2221 cgatgctttc ttccatggac tgtaccccct gtagcagaat tgattcacat aaactattat
     2281 tacattattt atgcatataa acaagcataa atttgatcag tacgtcatgg attgaacagg
     2341 ggtttttcag gcttcaggtc ggtgtttctc gagtcggccg ccttgagccc gaaatattcg
     2401 tcgatgatgt ctcttgccac gggagctgca ttggatcccc acacacctct ctcgataact
     2461 acggcaatgg ctatttccgg atcatcagcg ggtgcatagc agatgaagac tccattgggc
     2521 gagcgtccgc cgacgctttg tgtctgggct gaaccggtct ttccggctac ttcgaacggc
     2581 agtcctttga acgcttcctg cgcggtaccg tctatagccg tcgtaacggc taccatgccc
     2641 tctttgaccg cggctatggt cgactcctta agcgggatct ccgtataggt gggttcggtc
     2701 ctgttcacca cggacccgtc atatcttata acttccttga tgacgtgcgg cctgtacagc
     2761 ttgccgccgt ttgcaagggc agcggtgtag ttggcgagct gcagcggcgt gaaggagttg
     2821 tcgaactggc cgatcgaaac ctgggccgtg tcggcgggtc tccagatatc gttcctcagt
     2881 ttctttttca gttcaatgga cgacatcatt cccgaaacct cgttcggaag atctatgcct
     2941 gtctttttgc caagtccgaa acagttggcc cagtaggtca gggcttcgat gccggtcctt
     3001 gtgccaagct catagaagta caggttgcag gacgtctcca gccctctttt cagggaaagc
     3061 ggaccgtggc catacgtggg tttttcgagg cagaacaggt cccttcctcc tatacgggct
     3121 gtccccctgc agactatcgt gttcgaagtc ggggttatga cgcccttttc cagccctgcg
     3181 atggcagtga taggcttgaa tgtggaaccg ggcgcatagt tttcctgtat cgtcctgttg
     3241 agcaatgctt tcaggttctg ttcgtccttt gtcagttcta ttatcatctt ctgggcttcg
     3301 gtgtcttccg ggcctgcaag atagatagac gggtcgaagc tcgggtagct taccatcgtc
     3361 aggaccgctc cggtatggac gtccagcacc actgcggatc cggcgttggc gtcgccgtaa
     3421 ttgtaggcag gtcccttctt gtcgttcctg atcttctcaa tattgcgggc cagcgactct
     3481 atggcgacct tctgaaggtt cgtatctata gtgaggacca cgtcgttgcc gggtatctcc
     3541 tccattgtca caagcttctg gattatattc ccgtcagcat cgacctcaaa cgtgactctt
     3601 ccattcttcc cgcgcaggta cctttcagcg ctcttctcga ccccggtttg cccgatgagg
     3661 tcgtcattgt catatccctc atccttgagt tcatcatact gctttgaact tatcgcccta
     3721 atatacccga gcacatgagc ttcttcgtag gcgtcgatat actcccgcac cggctctgtc
     3781 atcgttatga tcccggggaa ctcatggttc ctctcctcta tctccgcaac ggtctccctg
     3841 cttacatccc tggccaggag caccattccg cccgtggtga aattccagtt gctgatcagc
     3901 agttcatacc tgaaaaccat tatcttatac gcctgctcgt ccgtgaacag cgtattgatc
     3961 ttgaattttt catctctcag gtatttgaaa agctcataag cgttatattt gacatcttct
     4021 tccttcatat tcagcctgtc ggcattgatc tgccaatcgc gtatctgctt ttcggtcatg
     4081 ccgttgaatt cgacaggcgg cccgtaggta aggaacttgg acagggtatt gacatgcccg
     4141 tccccgttct tctccagcag gtccgacagt ttgagcagca tctcgttcat ttcggaagtg
     4201 tttatgttcg tctttacgat atacagcgag tatccctgcc tgttgactgc aatgggcgtc
     4261 ccgtatctgt ccagtatctt ccctctcgga gcttctattt ccccttcctt gagtatcttc
     4321 ttctgcgcgt cggcaacata tttctctccc tccactatct ggaggttgaa aagccggaaa
     4381 actatcatga tgccgaatac catgaatccc aggcagagta tgtaatatct gtttctcagt
     4441 ttcttatcca aataatccat cctccttctg agaccatatg gctcaatact tccgagcagt
     4501 cgccgacatg tcgaaccatt tgccggcctt tacgagcaga gggaacagca gcagggatac
     4561 agcacagttg taccccgctt ccggcagtat gatcctcgtg aacgcgaacg caaagttgat
     4621 atcgccggac ataatgttgt ttatgacaaa cacgacagac tcataaacta cagaatatac
     4681 gaacgtaaaa aaggcgacaa ccagtaaatt ctccctgtat attttcctgc tcagcgatcc
     4741 ggccgctata ccggtgaaca tgcccagaag cccataaaag ccaagtactg cgccaaactg
     4801 catgtcgatg caaaggcctg aaaaaaggcc caccacagcg ccctcagtac tgttcctcac
     4861 caatgccgtg actatggtca aagtgatgag cagattcggc atgaccccaa gtatttttgc
     4921 acgctgaagc acagttgtct gcagcaggag gaaaaacacg ataaagacaa aatagaacaa
     4981 cagcttcttt ttcatcgtcc agcactgccc gttccatcat ttttttcgga cttaagtatg
     5041 aaaacttcct ccagcctcct gaaatccgca gtcggctgga tgatcgcata cctgccgagt
     5101 tcactgttgg tttttctcac ttcgatgact ttgccgattt ctatgccttt gggatagata
     5161 ccgccgacac ctgaagtttc aactatatcg cctacttcga tatcgacctc gatgggtatg
     5221 taaataagct tgcaaaggcc ctcctgcttt aattcaagat cgcctttcac aatggcatgt
     5281 ccgcctcccg gttttgctat ccatccggaa acggcgcttt cttcgtcaat gatggtctgt
     5341 atattcgatg tggatttatc ggtatcaaga actctcccaa caagacccct tgaaccagtg
     5401 acaacaggaa aatccgcccg gatcccgtcc tgttccccga catccacctt gaatacgctg
     5461 aaccagttac cgggctccac agcgatgata ttggcgccgg taatggtata atccgcgaat
     5521 tcggccttca ggttcagtgc acgcctcaat tcgtcattct ttgccttcag ttcggaatac
     5581 tccctgttct gtctcctcag ttcctcaact tccgctttca gagcttcatt ttctttcctg
     5641 agttcgtcta tatcctgaaa gtaagcgaaa aaatcttcta tttcacggcc tgccgatgtg
     5701 aaaaagccct ggacaggctt cagaggaaca cttaagatat tgctgagcca attcagctta
     5761 ctgcttttat ttgccgatac acctataacg accagcaacg ctatcgtaac cagagacact
     5821 atgaaccatt tgcttttaaa aagacgcagc aatggacaag tctcccttct atctcagttt
     5881 tctcggcgtt atcagcacac ttctcaacgt ttcgatttcc tcaagcactt tcccggtccc
     5941 gatggccaca cagtccagcg gattctcagc aatagtgacc ggcatgcctg tctcttcctt
     6001 gatcaacata tccagtccgc tcagcagcgc tcctccgccg gcaagcatta tacccttgtc
     6061 cataatgtca gcagccagtt ccggaggcgt cttttcaagc gtatacttga tagcgtcgat
     6121 tatcgagttt atgggttcct tcagcgcctc gtttatctcg ctggatgaaa gcgtaacgtt
     6181 tttgggaagc ccggttatca ggtctcttcc cctgacttcc atgtattctt ccttcggttt
     6241 gaggaaggca ttgcctatat ttatcttgat ctgttcggcg gtcctctcgc cgatcatcag
     6301 gttgtattcc ttctttacga aaagtacgat cgattcgtca aattcgtcgc cggcaaccct
     6361 cagcgatctg cttgtcacta tcccgccgag tgaaattacg gcaacttcgc tggtaccgcc
     6421 tcctatatcg actatcatgc tgccggaagg ctcttcgaca ggcaggttgg ctcctatcgc
     6481 cgccgccatt ggctcttcga taagataggc ctctcttgcg cctgccgcca gcgtggcatc
     6541 ttcgacggct ctcttttcga cttcggtaac gcccgacgga acgcagatga ctaccctggg
     6601 ttttgaaatg aagcctgtac ccatggctct tcttatgaaa tgcttcaaca tgctctgcgt
     6661 aatgtcgaaa tcggcaataa caccgtcttt cattggcctg atcgccacga tatcgcccgg
     6721 cgtcctgccg atcatgttct ttgcgtcatc gccgactgcc agtatctctc tcgtgttctt
     6781 atttattgcg acgacagaag gttccctgat cacgatgccc ctgcctttta catggaccag
     6841 cgtattggct gtccccagat ctattccgat atcctttgca aaaattccca taattatcct
     6901 ccctacgtct gcaaagaaca ggaccgttct tcagtctaca tcagtccctg ttcttttaaa
     6961 cttatgtacc ttccgtcccc aatgataatg tgatcgagga ccttgatccc cagtatactg
     7021 cctgcattta ccagcctgtg tgtcgtttcg atatcttccc tgctgggttc cggatctcca
     7081 ctgggatggt tgtggacaag cagcatgccg gaacaacctg ccctgaccgc ttcgctgaat
     7141 acctcccgcg gatggactat cgaagcattg aggcttccta ccgatacgtt cagatacttg
     7201 attatgtggt tcttcgtatt cagcaggatc accttgaaca cttccttctt aagatgcctc
     7261 atctcctcca tcagcagcat gctgacatcc gccggagatt tgatcgtaac tttattattg
     7321 tttataaaag aagcggatat ccttttcgac agttccatga ccgcttttat ctgcaccgct
     7381 tttacccttc cgatgccttt cactttccgg agttcttcca cggataagtc atggagatgc
     7441 gctatccctc ccttgttttc accaagcgac agcaatcgct gcgccagtgc cactgacgtt
     7501 tcttcagcgc ttcccgtcct gatgatcact gccagaagtt cagcgttcga caggaactca
     7561 gggccgtact tttccagctt ctcataaggt ctttcactag ctggtatatc cttcattcta
     7621 agtctgtatt ccattttggt caaccccgca tttcttaaaa aataattaca catcactcct
     7681 ttcattgtac cattttttac atttattttc tacattttgg aaattttcgc tgttcatccg
     7741 caggccgaaa cgccaaattc cttcaacagg cgatagaccc gccgtaaagg aagcccgacg
     7801 acatttgagt aacatccgtc aaggctctcg acaaaaatgg ccgcccgccc ctggatggca
     7861 taagcgccgg cttttccgta cggctcgccc gacgatatgt aagcatcgat ttccctgtca
     7921 gtaatttcgg aaaatctcac cattgtcact tcatgatcgg tccgtgcctt gccgtccgaa
     7981 gcatcgacca ggcaaatacc ggtaacgacc ttatgttccc gccccttaag cttattgagc
     8041 atccttcggg cgtcttcctc atccctgggt ttgccgaata tctcatcatc acagacgact
     8101 atcgtatccg caccgagcac cagtccgctc tccagcttcg atgccacagc catcgctttc
     8161 atgtaagcaa gctgctctgt cttctgttcc ggtgttcctg acaagtcagc attctcttca
     8221 tctatgtttc ccggcaccac ttcgaagggt atcccgacct gggccagcag ttcccttcgc
     8281 cttggcgacg ccgaagccag tattatctta gtcataggtt aagggacact tccaatttcc
     8341 tcacattgac acttctacgg gttttcaaaa ggggaaattc ctcttcaatc gaagcccggc
     8401 ttcctgcaga ggagcgtcca ctgtcctccg gctggtctca gaaatgtccc ttttttgatt
     8461 caccttcctt atctcatgcc aaagtttgtc cttctgtctc ctaattattt atcttatcat
     8521 aaaaataaac agatataaat taatttcaat aaaagggaac ataaataacg aacctttgtt
     8581 attccggaaa gtggaaaaga aaaaagagga gtggtaatag gaaaagggac acttctcaac
     8641 tgcgaagtat tacttcctgc agaggagtgt ccagtgtcag ctaatcaaaa agggacattc
     8701 ctcaactcat ggagcacttc ttcctgcaga ggagtgtcca tcgtaagcta aaggaaaagg
     8761 gac
//
LOCUS       DAOSCC010000096         8687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5302_length_8687_cov_4.313832, whole genome shotgun sequence.
ACCESSION   DAOSCC010000096 DAOSCC010000000
VERSION     DAOSCC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_8687_cov_4.313832"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLG72_06440"
     CDS             complement(<1..646)
                     /locus_tag="PLG72_06440"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ80471.1"
                     /translation="MRLVLLGAPGAGKGTQAVIISKRLQIPHISTGDIFRSNIKNETP
                     LGKLAKQYIDSGALVPDEVTVAIVKDRLAEPDCANGFILDGFPRTIQQAESLDRILEE
                     LGTKLDKVIDIHAGDDVIIKRLSGRRVCPECTSIYHIRTSEMARNGICENCGAKLIQR
                     EDDREETVRKRLETYHDQTEPLISYYREKGILVRVQGEGSAAETTAEIYQVLGMT"
     gene            complement(665..1963)
                     /gene="secY"
                     /locus_tag="PLG72_06445"
     CDS             complement(665..1963)
                     /gene="secY"
                     /locus_tag="PLG72_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOJ80472.1"
                     /translation="MGEMFQTIRNAWKIPELRKKILFTIAMLIVFRFGNRIPNPGLDP
                     EAFSRLIDMFGSLGSVVDIVSGGAFKIASIFAMGISPYINASIIIQLLTVAIPALEKL
                     QKEGQEGQKILQQYVRYGAVILGIVQAVALYFTVLPHGGIIDNGSVWSFLLVVFSFAA
                     GTAFLMWLGEQITEHGIGNGISLIIFAGIISRGPSMVEGIIVNYQLGNFGTGVWGILA
                     IIGIVILFIAVIAAVIWVQQAERRIPIQYAKRVVGRKMYGGQSTHLPIKVNLAGVLPI
                     IFAMSIMMLPSTIITLFFANKDNWFVRLFKNPERSLAYIIIYALLIIFFTFFYSVIQF
                     NPVEIANNLKKNGGFIPGYRPGKATSDYITKVLNRVTWFGGIFLALVTIAPIILGNVT
                     KIQGIWFGGTAILILVGVAIETVQAIEAQMLMRHYKGFLE"
     gene            complement(1965..2408)
                     /gene="rplO"
                     /locus_tag="PLG72_06450"
     CDS             complement(1965..2408)
                     /gene="rplO"
                     /locus_tag="PLG72_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOJ80473.1"
                     /translation="MKLHELRPAEGARKLPNRKGRGHASGNGKTAGRGHKGQNARSGG
                     GVRPGFEGGQMPLYRRIPKRGFNNKKFAKVYAEINVSALNVFEDGTVVNAELLKEKGL
                     IKEIYDGVAVLGNGELTKKLTVQAARFTRTASQKIEAAGGKKEVV"
     gene            complement(2423..2602)
                     /gene="rpmD"
                     /locus_tag="PLG72_06455"
     CDS             complement(2423..2602)
                     /gene="rpmD"
                     /locus_tag="PLG72_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOJ80474.1"
                     /translation="MAKLKITLIKSTCKCNKNQKATVEALGLKKIRDFVEHEDNPQIR
                     GMIKRVSHLVSVEEI"
     gene            complement(2615..3115)
                     /gene="rpsE"
                     /locus_tag="PLG72_06460"
     CDS             complement(2615..3115)
                     /gene="rpsE"
                     /locus_tag="PLG72_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOJ80475.1"
                     /translation="MQRIDAGTLDLKEKVVKIGRVAKVVKGGRNFRFSALVVVGDENG
                     YVGAGMGKAAEIPDAIRKGIDDAKKNLIKVPLVDTTIPHEVIGHYGAGKVLLKPAGPG
                     TGVIAGGAVRAVLELAGVRDIRTKSLGSNNPINMVNATMEALRQLKTVEEVAVLRGKK
                     PEEILG"
     gene            complement(3163..3531)
                     /gene="rplR"
                     /locus_tag="PLG72_06465"
     CDS             complement(3163..3531)
                     /gene="rplR"
                     /locus_tag="PLG72_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ80476.1"
                     /translation="MIIKVGKNEIRQRKHERVRKKIFGTPERPRLNVFRSLKHIYAQI
                     IDDTTGTTLVAASTMEQSLKDKVTYGGNKQAAREVGKLVAQKALEKGIKKVVFDRGGY
                     LYHGRVKELAEGAREGGLEF"
     gene            complement(3553..4095)
                     /gene="rplF"
                     /locus_tag="PLG72_06470"
     CDS             complement(3553..4095)
                     /gene="rplF"
                     /locus_tag="PLG72_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ80477.1"
                     /translation="MSRIGKKPIDIPAGVNVDIKDNVVTVKGPKGTLTKEMHKDMAIK
                     KEGDQIIVSRPSDEKIHKSLHGLTRTLINNMVEGVTKGFEKALEINGVGYRAQKQGNK
                     LVLNIGYSHPVEMEEPAGITVEVPAPNKIVVKGCDKQAVGEFAAKIRAQRVPEVYLGK
                     GIKYENEVIRRKAGKTGAKS"
     gene            complement(4229..4627)
                     /gene="rpsH"
                     /locus_tag="PLG72_06475"
     CDS             complement(4229..4627)
                     /gene="rpsH"
                     /locus_tag="PLG72_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ80478.1"
                     /translation="MQITDAIADMLTRIRNASSAKHESVDIPASNIKKDMARILLDEG
                     YIKDVEFIEDGKQGIIRIFLKYTGNKQSVISGIKRISKPGLRVYAKKDELPKVLGGLG
                     IAVISTSKGVMTDKKARAEGVGGEVLAFIW"
     gene            complement(4653..4838)
                     /locus_tag="PLG72_06480"
     CDS             complement(4653..4838)
                     /locus_tag="PLG72_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOJ80479.1"
                     /translation="MAKKSLVVKQKRTPKYSTRAYNRCQICGRPHAYMRKFGICRLCF
                     RVLAYKGQIPGVKKASW"
     gene            complement(4862..5404)
                     /gene="rplE"
                     /locus_tag="PLG72_06485"
     CDS             complement(4862..5404)
                     /gene="rplE"
                     /locus_tag="PLG72_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ80480.1"
                     /translation="MSRLKDKYINEVRPAMTAKFKYKSPMQIPKLEKVVINMGVGEAK
                     ENPKAMDAAVNDLMIITGQRPVVTKAKKSISNFKLRQGMNIGCKVTLRSDKMYDFVDK
                     LFNAALPRVRDFRGVNPNSFDGRGNYTLGVKDQLIFPEIEYDKVDKARGMDIVFVTTA
                     KTDEEARELLKLLGMPFANR"
     gene            complement(5436..5783)
                     /gene="rplX"
                     /locus_tag="PLG72_06490"
     CDS             complement(5436..5783)
                     /gene="rplX"
                     /locus_tag="PLG72_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ80481.1"
                     /translation="MANKVHVKTGDMVYVLSGKDKGKKGKVLAVNPDDMTVIVEGVNI
                     TKKHRKPRGRLQEGGIISQESPISSSKVMLVCSRCKSPTKIAKVIRENGQKDRVCKRC
                     GEVIDTQKEAAEE"
     assembly_gap    5796..5805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5851..6219)
                     /gene="rplN"
                     /locus_tag="PLG72_06495"
     CDS             complement(5851..6219)
                     /gene="rplN"
                     /locus_tag="PLG72_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOJ80482.1"
                     /translation="MIQVQTRLKSADNTGAKELMCIKVLGGSKRKYANIGDVIVCSVK
                     NATPGGVVKKGEVVKAVIVRSKKGVRRPDGSYIRFDENAAVIIKEDKNPRGTRIFGPV
                     ARELRDKEYMKILSLAPEVI"
     gene            complement(6257..6514)
                     /gene="rpsQ"
                     /locus_tag="PLG72_06500"
     CDS             complement(6257..6514)
                     /gene="rpsQ"
                     /locus_tag="PLG72_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOJ80483.1"
                     /translation="MAERGLRKTRIGKVVSDKMDKTIVVAIETSVKHPLYKKIVKRTY
                     KLKAHDENNECRVGDKVKVMETRPLSKDKRWRLVEIVEKAR"
     gene            complement(6533..6736)
                     /gene="rpmC"
                     /locus_tag="PLG72_06505"
     CDS             complement(6533..6736)
                     /gene="rpmC"
                     /locus_tag="PLG72_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOJ80484.1"
                     /translation="MKASELRNMTQEELQKELNELKSELFKLRFQLVTNQLENPMKLR
                     DVKKNIARVKTVMREREIKSAKA"
     gene            complement(6723..7163)
                     /gene="rplP"
                     /locus_tag="PLG72_06510"
     CDS             complement(6723..7163)
                     /gene="rplP"
                     /locus_tag="PLG72_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOJ80485.1"
                     /translation="MLMPKRVKRRRVHRGRMKGIASRGNYVAYGEYGLQAVEPAWVTS
                     NQIEAARIAMTRFTKRGGQVWIKIFPDKPVTKKPAETRMGSGKGSPEYWVAVVKPGRV
                     LFEIAGVPEETAREAMRLAMHKLPMKCKFIAREKEMGGETNESQ"
     gene            complement(7163..7831)
                     /gene="rpsC"
                     /locus_tag="PLG72_06515"
     CDS             complement(7163..7831)
                     /gene="rpsC"
                     /locus_tag="PLG72_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ80486.1"
                     /translation="MGQKVNPHGLRIGIIKDWDTKWYASDKNFSEYLVEDHKIREYIK
                     NRLYAAGVARIEIERAANKVKINVHTAKPGIVIGKGGQGIEELRKEIEGLTGRNVLIN
                     ITEIKVPEINAQLVAENIAAQLEKRISFRRAMKQAMARAMKLGAKGIKTAVSGRLGGA
                     EIARTEHYHEGTIPLQTLRADIDYGFAEANTTYGKLGVKTWIYKGEVLPAVKKDNKAE
                     GGDK"
     gene            complement(7865..8239)
                     /gene="rplV"
                     /locus_tag="PLG72_06520"
     CDS             complement(7865..8239)
                     /gene="rplV"
                     /locus_tag="PLG72_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ80487.1"
                     /translation="MLTKKERKILGIGKDEGRAILRHARISDRKAKIVLDLIKGKSID
                     EAYAILRYTPKAASELIYKLLKSAEANAVNNNDLNRDALYVAEAYATQGTTLKRIQPR
                     ARGSAYRIRKRTSHITVVVKER"
     gene            complement(8485..>8687)
                     /gene="rpsS"
                     /locus_tag="PLG72_06525"
     CDS             complement(8485..>8687)
                     /gene="rpsS"
                     /locus_tag="PLG72_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ80488.1"
                     /translation="KVIKTWSRASTIFPQMVGHTIAVHDGRKHVPVYITEEMVGHKLG
                     EFAPTRTFRGHGHHTEKSTALK"
BASE COUNT         1747 a         2459 c         1725 g         2746 t
ORIGIN      
        1 acgtcatccc cagcacctgg tatatttctg ctgtcgtctc cgcggcgctg ccttctccct
       61 ggacccggac caggattcct ttctccctgt agtagctgat aagcggttcc gtctggtcat
      121 gataggtctc aagccttttc ctgaccgtct cttccctgtc gtcttctctc tgtatcagct
      181 ttgcgccgca gttttcacat atgccgtttc gggccatttc gctggtccgt atgtgatata
      241 tcgaagtaca ctcagggcac actcttcttc ctgaaagcct ctttattatc acatcgtccc
      301 cggcatgtat gtcaatgacc ttatccagct tcgtgccaag ttcctcaagt atcctgtcaa
      361 ggctttcagc ctgctggatc gtgcgcggga agccatccag tatgaagccg tttgcacaat
      421 ccggctcagc aagcctgtct ttgactatgg ccacagtgac ttcgtcggga acaagagccc
      481 cgctgtcgat gtactgcttc gccagcttcc ccagcggggt ctcattcttt atattgcttc
      541 tgaaaatgtc accggttgaa atgtgcggga tctgcagtct cttcgagata atgactgcct
      601 gtgttccttt cccggcaccc ggcgctccca atagtacaag tctcataata cgttacactc
      661 cgctttactc aaggaaacct ttatagtgtc tcatcagcat ctgggcttcg attgcctgca
      721 cagtctctat tgcaactccg acaaggatca gtatggctgt accgccaaac catattccct
      781 ggatcttcgt gacgttgccg agaattatcg gagcgattgt caccagcgca aggaatatgc
      841 cgccgaacca agtgactctg ttcagcactt tcgtgatata gtccgaagtc gcttttccag
      901 gcctgtaccc tggtatgaag ccgccgttct tcttcaggtt gttcgcgatc tcaacagggt
      961 tgaactgtat cactgagtag aagaacgtga agaatatgat aagcaaagcg taaattatga
     1021 tatatgccag gcttcgttca ggattcttga acagcctcac gaaccagttg tctttgtttg
     1081 caaagaacag cgttatgata gtcgacggaa gcatcattat ggacattgcg aaaattattg
     1141 gcagaacacc tgcgagattg acctttatcg gcagatgtgt actttgaccg ccatacatct
     1201 tcctgccgac gacgcgcttt gcatactgga taggtatcct tctttcagcc tgctgcaccc
     1261 agatgactgc tgcgatcacg gctatgaaca gtattactat accgattatg gcaagtatgc
     1321 cccatactcc ggtaccaaaa ttgccaagct ggtaattgac gattatgcct tccaccatcg
     1381 agggccccct ggatatgata ccggcaaata tgatcagcga tataccgttg ccgataccat
     1441 gctccgtgat ctgctcaccg agccacatca ggaacgcggt acctgctgcg aacgagaata
     1501 ctaccaaaag gaagctccac acacttccgt tatcgattat accgccatgc gggagcacag
     1561 tgaaatacag ggcaacagcc tgtacaatgc cgagtatgac ggctccgtat ctgacatact
     1621 gctgcaggat cttctgacct tcctgtccct ccttctggag cttttcaagg gcaggtatgg
     1681 caaccgtcag aagctgtatg ataatcgaag cattgatata cggtgatatg cccatagcaa
     1741 aaatcgatgc tatcttaaat gcgccgcccg atacaatatc aacgacactt ccgagcgagc
     1801 cgaacatatc gatcaacctg gagaatgcct ccgggtcaag acccggattc ggtatacggt
     1861 ttccgaacct gaacacgatc agcattgcta tggtaaacag gattttcttg cgaagttccg
     1921 gaattttcca tgcattacgt atggtctgga acatttcgcc cattttatac cacctctttc
     1981 ttccctcctg cagcttctat cttctgggaa gctgtcctgg taaatctggc agcctgaaca
     2041 gtgagcttct ttgtgagttc accgtttccg agcactgcta cgccgtcata gatctccttg
     2101 atcaggcctt tttccttcaa gagttctgca tttaccacag taccgtcctc gaacacattc
     2161 agcgccgaga catttatttc agcgtaaacc ttggcaaatt tcttattatt gaatcctctc
     2221 ttcggtatcc ttctgtagag aggcatctga ccgccttcaa aacccggcct tactccgccg
     2281 ccggagcgtg cattctgacc cttgtggccg cgccctgctg tctttccgtt acctgatgcg
     2341 tgccctcttc ccttcctgtt cggaagcttc cttgcacctt ctgccggtcg caattcgtgt
     2401 aatttcactt tcagcacctc ccttatattt cttctacaga taccagatga gatacccttt
     2461 tgatcatccc tctgatctga gggttatcct catgttccac aaaatctctt atcttcttca
     2521 aaccaagcgc ttcaacagta gctttctggt tcttattaca cttgcatgtg ctctttatta
     2581 atgtaatctt cagctttgcc actgtagtgt cctcctaacc caatatttct tccggcttct
     2641 tgccgcgcag tacggccact tcctcaactg ttttcagttg cctcagagcc tccatggttg
     2701 catttaccat gttgatcgga ttgttggatc ccaatgactt ggtccttata tcacgaactc
     2761 cggccagttc gaggactgct cttacggcac ctccggcaat gacaccggta ccggggcctg
     2821 ccggcttgag caataccttt cctgctccgt aatgtcctat aacttcgtgg ggtatggtgg
     2881 tatctacgag cggaaccttt atcaggttct tcttcgcatc atctatacct ttcctgatag
     2941 catcaggaat ttcagctgcc tttcccattc ctgccccaac atagccgttt tcatctccga
     3001 ctactacaag agcactgaaa cggaagtttc tgccgccctt gacaaccttg gcaacgcggc
     3061 ctattttaac gaccttttcc ttaagatcca gtgttccagc atcgatacgc tgcaatttcc
     3121 atccctccta attcaaacct ggttactgtc ggtgcagccc gcttaaaact caagcccgcc
     3181 ttctcttgcg ccttctgcaa gctctttcac tcttccatgg tacagatatc cgcctctgtc
     3241 gaatacaacc ttctttatcc ccttctccag tgctttctgg gctaccagtt tgccgacttc
     3301 ccttgctgcc tgtttgttgc cgccgtaggt aaccttgtct ttcagcgact gttccatggt
     3361 agacgctgct acgagcgttg ttccggttgt atcatcgatt atctgtgcgt atatatgctt
     3421 taagcttctg aaaacattga gcctcggacg ctcgggagta ccgaatatct tcttgcgtac
     3481 tctctcatgt tttctttgcc tgatttcatt ttttccaacc ttgattatca tctcttccta
     3541 actccctcct gcttaggatt tagcaccggt cttgcctgct ttgcgcctga tcacttcgtt
     3601 ctcgtacttg atacccttgc cgagatagac ctcaggtact ctctgtgccc ttatctttgc
     3661 ggcgaactct ccaactgcct gtttatcgca gcccttgacg actatcttgt tcggggcagg
     3721 cacttctacg gttatacctg caggctcttc catttccacc ggatgcgaat agccaatgtt
     3781 gagtaccagt ttatttccct gtttctgggc tctgtaaccg acaccattga tctcaagcgc
     3841 cttctcaaaa ccctttgtga caccttccac catattgttg ataagtgtcc tcgtcaatcc
     3901 atggagggac ttgtggatct tttcatccga aggccttgat acgatgatct ggtctccctc
     3961 tttcttaatg gccatgtcct tgtgcatttc cttggtcaaa gttccctttg gaccctttac
     4021 tgtaacaaca ttgtctttga tatctacatt cacacctgcc ggtatatcaa taggtttctt
     4081 gcctatcctt gacatcaaag cacctcctgt tcttgagatt atgagccctc tttaggaaat
     4141 gtccgcgaga gcccttttca gatattcagt gcgaaccaat ccatatatga atttccgccg
     4201 tcgattcgca gatacattgt aaatcaaact accatataaa agcaagcact tctccgccta
     4261 cgccttcggc tcttgccttc ttgtcagtca tcacgccctt ggatgtggaa atgactgcaa
     4321 tgccaagccc gccgagtacc ttcggcagtt cgtccttctt ggcatagacc ctaagtccgg
     4381 gtttgcttat cctctttatc cctgaaatga cgctctgctt gttgcctgta tatttgagga
     4441 atatcctgat aatgccctgc ttgccgtctt ctatgaactc gacatccttg atgtagcctt
     4501 cgtccaacag tatacgagcc atgtcctttt tgatattgga tgccggaatg tcgaccgatt
     4561 cgtgttttgc cgagcttgca ttcctgatcc tggtcagcat atcggcgata gcgtctgtta
     4621 tctgcatgtt ttcaacctcc tcccaaaact gattaccagc tggctttctt aactcccggt
     4681 atttgacctt tgtaagccag aaccctgaag cacaggcggc aaatcccgaa cttcctcatg
     4741 tatgcatgag gcctgccgca gatctggcat ctgttgtaag ccctggtact gtattttgga
     4801 gttcttttct gttttacgac caacgatttc ttcgccacgt atatcctcct tccaccactc
     4861 tctacctgtt cgcaaacggc attcccagaa gcttcaggag ttctctcgct tcctcgtccg
     4921 ttttggcagt ggtaacaaat acgatatcca ttccccttgc tttatctacc ttgtcgtatt
     4981 cgatctcagg gaatatgagc tggtccttga cgccaagcgt gtagttccct ctcccgtcga
     5041 atgaattggg atttactccc ctgaagtctc tgacacgcgg cagagcagcg ttgaacagct
     5101 tgtcgacgaa atcatacatt ttgtcgctgc ggagcgtaac tttgcaccct atgttcatac
     5161 cctgcctcag tttgaaattc gagatggatt tctttgcttt ggtcacaacc ggtctctgtc
     5221 ctgtaatgat catcagatca ttaactgcag catccatagc tttgggattt tcctttgcct
     5281 cgccgactcc catgtttatt actacctttt caagttttgg gatctgcatc gggctcttat
     5341 atttaaactt tgccgtcatt gccggtctta cttcgttgat gtatttatcc ttaagccttg
     5401 acatcagaca ttaacctcct ttcaagaagc atatgttatt cttctgctgc ttccttctgt
     5461 gtgtctatta cctcgccgca tcttttgcat acacggtctt tctgcccgtt ctcacggatg
     5521 actttggcta tcttggtcgg gcttttgcat cttgagcata ccagcatgac cttcgaactg
     5581 ctgatcggcg attcctggct tattattccg ccctcctgca gccttcccct cggttttctg
     5641 tgtttcttgg ttatattaac gccttcaacg attacagtca tatcatcggg gttaacagca
     5701 agaaccttgc cctttttgcc cttatccttg ccagagagaa catataccat atccccggtt
     5761 tttacatgta ccttgtttgc cactttagcc gcctcnnnnn nnnnngatgc tatatttcgg
     5821 tcatgtgtca agcgacactg aacattccgt tcagataacc tccggtgcga gtgacagtat
     5881 tttcatgtat tccttgtctc tcagttctct cgcaacaggt ccgaatatac gtgtccctct
     5941 aggatttttg tcttccttta tgataactgc tgcgttttcg tcaaacctga tatatgaacc
     6001 gtccggtctt ctgactccct ttttggaacg aactataaca gctttgacaa cttcgccctt
     6061 tttcacaact ccgccgggtg ttgcattttt aactgagcaa acaataacgt ctcctatatt
     6121 tgcatatttc ctcttggagc ctccgagcac ctttatacac atcagttcct tggctccggt
     6181 gttgtctgct gatttcagtc tcgtctggac ctgaatcata ccggtacctc cttccagtta
     6241 cttcgaaata ctgctgtcaa cgagcctttt caacaatctc gacgagtctc caccttttat
     6301 ccttgctgag cgggcgggtc tccatcacct tgaccttgtc gcctacccgg cattcgttgt
     6361 tctcatcgtg tgccttcagc ttataagtcc tcttaacgat ctttttgtac aaaggatgct
     6421 ttacgctggt ctctatcgca acgacgatgg tcttatccat cttgtcactg actaccttgc
     6481 ctatcctggt tttcctcaat cctctttcag ccaagcagga ttcctccttt cattaagcct
     6541 ttgcactttt tatttctctt tccctcatca cggtcttgac tcgggctata ttcttcttga
     6601 catccctgag cttcatcggg ttttccagtt ggttcgtcac aagctggaaa cgaagtttaa
     6661 aaagctcaga cttaagttca ttcagttcct tctgcagttc ttcctgcgtc atatttctca
     6721 actcactggc tttcattcgt ttcaccaccc atttctttct cgcgggctat gaacttgcac
     6781 ttcataggga gcttgtgcat ggcgagcctc atagcttctc ttgccgtttc ctccggtacc
     6841 cccgcgatct caaataatac tcttccgggc ttcacaactg caacccagta ctccggtgaa
     6901 cccttaccgc ttcccatacg ggtctcggcc ggcttcttcg tgaccggctt gtcagggaat
     6961 atctttatcc agacctgacc gcctcttttc gtaaaacgtg tcattgctat cctggcagct
     7021 tctatctggt tgctcgttac ccaggcgggc tcgacggcct gcagaccata ttcgccgtat
     7081 gcaacgtaat tgcccctgga tgcgatacct ttcattctgc ccctgtgtac tctcctgcgt
     7141 ttaaccctct tcggcatcaa cattacttat cgcccccttc tgccttgtta tccttcttaa
     7201 cggccggaag cacttcacct ttatatatcc acgtctttac tccgagtttt ccgtaagtcg
     7261 tgtttgcttc ggcgaaacca taatctatat ccgctctaag tgtctgcagc gggatggtcc
     7321 cttcgtgata atgctctgtc cttgctattt cagcaccgcc aagccttcct gaaacggcgg
     7381 tcttgatacc ttttgctccc agtttcatgg ctcttgccat cgcctgtttc atcgctcttc
     7441 tgaaagatat cctcttttca agctgtgcgg cgatgttctc ggcgacgagc tgggcattga
     7501 tctcgggaac ctttatttca gtaatattga tcaggacgtt cctgccggtg agaccttcga
     7561 tctccttgcg cagctcttct ataccctggc cgcccttgcc aatgactatg ccaggttttg
     7621 cagtgtggac atttatcttg accttgtttg cggctctctc tatttcgatc ctcgcaacgc
     7681 ctgcggcata aagcctgttt ttgatatact ctcttatttt gtggtcttca accaggtact
     7741 cgctgaagtt tttatcgctt gcataccatt tggtatccca atccttgatt ataccgattc
     7801 gcaatccatg cggatttacc ttctggccca tcatcgaacc tccttcttag cgtattgccg
     7861 acccttatct ttccttcact accactgtga tgtgactggt cctcttgcgt atcctgtatg
     7921 cgcttcctct tgccctgggc tgtatcctct tcagagtcgt gccctgagtt gcatatgctt
     7981 ccgctacata aagcgcatct ctgttgaggt cgttattgtt cacagcattg gcctctgctg
     8041 atttaagaag cttgtaaatc agctcggaag cagctttcgg tgtatatctc aagatagcat
     8101 aagcttcatc gatgctcttg cctttgataa gatccagaac tatctttgcc ttcctgtcgg
     8161 agatccttgc atgcctgagg atggctctgc cttcatcctt gcctatcccg agtatcttcc
     8221 tctccttctt tgtaagcagc gcaggtttct gaaactgtct gctggtcttg ttatattgtt
     8281 ccagtatctg atccttgttc ttcaaaagct cttctttgga caaaaccttc ttttccaact
     8341 ggaattcctc ctttcggcat taagcttgtt cgtgcacgta aatcgctgtg tcggcactgc
     8401 gccggggctt gcagcttcag atgctcctgc gcccgtacgg tccgtcgttg ctgcagaaaa
     8461 catcatttgc cgtatgcgac cggcttattt caatgctgtc gacttctccg tgtgatgtcc
     8521 gtgccctctg aatgtcctgg tcggtgcaaa ttcacccagc ttatgcccta ccatttcctc
     8581 agttatataa accggcacat gctttctgcc gtcatgaact gcaatagtat gccctaccat
     8641 ctgagggaat atcgttgacg ccctggacca ggtctttatg accttct
//
LOCUS       DAOSCC010000097         8669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5318_length_8669_cov_4.761783, whole genome shotgun sequence.
ACCESSION   DAOSCC010000097 DAOSCC010000000
VERSION     DAOSCC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5318_length_8669_cov_4.761783"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="flgL"
                     /locus_tag="PLG72_06530"
     CDS             complement(<1..581)
                     /gene="flgL"
                     /locus_tag="PLG72_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOJ80489.1"
                     /translation="MRITNNMLINNMINYIGNNLTRMDKYQAQLATGKKIQVPSDDPV
                     VAARALKLRTDVAEIQQYQRNLKDARSWLDITESTLSDIGDIFQRVRELIVGSDAIES
                     PEDLQATKNELIQLRTQLINLGNSAYAGRYIFTGFKTDQKLLNDDGTFAVDVSNTEQI
                     YYQIGLADSINVNVLGGDLFNDGTDAATGSTGK"
     gene            complement(655..2280)
                     /gene="flgK"
                     /locus_tag="PLG72_06535"
     CDS             complement(655..2280)
                     /gene="flgK"
                     /locus_tag="PLG72_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ80490.1"
                     /translation="MSGSFFGLNVAVRGLFAAQRNLDIVNHNLNNINTPGYSRQQAIQ
                     VASRPMALLDGTGMLGTGAEVIGVERIRDEYLDFKYWGENPSYGEWSVKDEILKNLEV
                     LFNEPSDSGFTAIMSSFFDSLQELSKDPSSAAVRSLVRQNGVTLAKFFNNLATKLEEL
                     QYDINYRIQTKVEEINSLAVQIQQLNRQIYITELSGNTANDMRDQRTVLVDRLSKIIN
                     IEANEIVVGYTPDGREDKHFVITVSGKALVDHFSVSKLALEQRPDDKMVNQEDIPNLY
                     EVKWADGNKLNVKGGELRGLLDLRDGNEGSVNPDNGLVSPNYKGVPYYIKKLNEFVRV
                     FARAFNEGYIDLDGDREFDPGEFGTGHSSGYGYNSDLTGIRFFTMTDENGKVLSSDEF
                     LNGESDPALIYAAYEKITAKNFCVSADILGDYNLIAASDTQYEAGNIEVLNVLLSFRR
                     NSDMFSEGAPEDFMKSLVTTLGIDSQQARNFSMNQKAVVNQIENRRLSNSGVSMDEEM
                     ANLVKFQHAYNAAAKMIQTMNEVYETLINGLFV"
     gene            complement(2287..3747)
                     /gene="flgK"
                     /locus_tag="PLG72_06540"
     CDS             complement(2287..3747)
                     /gene="flgK"
                     /locus_tag="PLG72_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOJ80491.1"
                     /translation="MSFASYEIARSGLTVNERGLYVTGHNIANVNTPGYVRQQAMIKN
                     GPVNTVLTRGGLMQVGLGADIQEIRQIRNTFLDNMYRKENSALGYWETRQKTFIDLQA
                     ILGEPMESGLQNILNQFWDAWQELAKEPDSLTVRALVRQRSEALVQYVNHLGEQLDRL
                     QNDLNNEIAVRIGEINEITREIAELNVTILKNEVSGDTAADYRDQRNKLIDRLTRLAD
                     VNVIEMQDGQVDITLGGYFLVQRGISTDLYAEERNPGDIFYVAKLAGTDIEVPVKSGI
                     LKGLMESRGEIPVSGGVTSEDTMNIVSDVKNKLNTLISVMLNEINNLHKSGMNIKDPP
                     EYGEDIFVAINPAKPLQMGNITLNPNLSDLSVIAASKTGMAGDNTIALEIANLRNKAL
                     ITSKTGLVSIDDYYQYIILTVGNKASEAVNITGSQQNLVQAIDMQRTAIAGVSMDEEM
                     TNMLKFKFAYDAASRVLNIIDSMMETVVNRMGLTGR"
     gene            complement(3770..4270)
                     /locus_tag="PLG72_06545"
     CDS             complement(3770..4270)
                     /locus_tag="PLG72_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOJ80492.1"
                     /translation="MDTDMVKTLIEILDNEAAIYEALLKLSHEKTDVIVQGKVTELEN
                     ITKVEQSMILRLAKLEGNREKIVEKLSEEIGMDADSITVTELEKVLPKDQSKELAECQ
                     KKLTGIFSDLRNSNDLNARLIKNSLDYINFSVNVLSNVGSPGDIYGKSGQSNDPGKRN
                     FFDVKF"
     gene            complement(4335..4622)
                     /locus_tag="PLG72_06550"
     CDS             complement(4335..4622)
                     /locus_tag="PLG72_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOJ80493.1"
                     /translation="MRIPGDVPKITGIYGSQKKVNRVGSTGSVASRKDVLSISSEAKD
                     FQAVFRALREVPDIRQDKVDDIAGRNETGQYDVKGRDIADKVIRSVFDRKA"
     gene            complement(4724..5158)
                     /locus_tag="PLG72_06555"
     CDS             complement(4724..5158)
                     /locus_tag="PLG72_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOJ80494.1"
                     /translation="MPDVRNCRKCGKIFNYIGGAPICPACREKEEEDFQKIKKYLYEN
                     PGASLTQVSTELEVSVEMIKRFLREGRLEIANDDGNLLLECENCGKAIKTGRYCPECE
                     RILASGFKSAADQMKLDLDSRGTADRRSLGLRYLNKEYERDK"
     gene            complement(5176..5844)
                     /locus_tag="PLG72_06560"
     CDS             complement(5176..5844)
                     /locus_tag="PLG72_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ80495.1"
                     /translation="MFESIVNLIYPPRCIFCQQLLDYNAIIHICGSCYGKLPFSGSAI
                     IRTSQEDEAGHCDGALSVFDYTGMVKESLIRFKFYNKPGYYMTYAKLIADKLKKLTNI
                     SQYDMVMSVPLHKHKEFIRGYNQAYLISKALSRIIRLPECSKVLKRERYTEAQSLLDR
                     QKRNQNVKGAFSVKSPKKVKGKSILLVDDILTTGSTLEECSRVLKQAGAVKVFAVVVA
                     TGRK"
     gene            complement(5871..8099)
                     /locus_tag="PLG72_06565"
     CDS             complement(5871..8099)
                     /locus_tag="PLG72_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOJ80496.1"
                     /translation="MDPALVEGVIEEIIYKNEANGYTVCEIASGGKIITAVGYMPFVN
                     EGETIKANGRWVKHPDYGDQFKVEMYEKVLPHTAESIEKYLASGVIKGVGPVTARKIV
                     ERFGTSALDVISNEPERLSEIKGISLAKAMDIGQALNEHRGLQDVVMFLQEYGVSPSV
                     CIRIFKAYGNKSVEMIRENPYRLCEDVFGIGFKTADRIAMRLGVDPASLFRIRSGIRY
                     ILSRAASNGHTYLPENMVKMYTSQLLGIPEVDIEDALVSLVFDKAVCTEKTEDGSNVY
                     LSAFYTAETGVCKRLLSLSRTSHARDFEDLDEKIRDFEEREGIQLDRKQREAVREAMR
                     NGVMVITGGPGTGKTTIIKCILRLLSEYGYRAALAAPTGRAAKRMTEATGYEAKTIHR
                     LLEIGYADDESELVFQRNEANPLEADTVIIDEMSMVDIILMHHLLKAISPGARLVLVG
                     DADQLPSVGPGSVLHDIISCSGIKTVRLTEIFRQAEESLIIVNAHRINRGEAPILNAK
                     DKDFFFLPRSGQENILRTVIDLCVRRLPDTYGLDPMKDIQVLSPTKKGPAGVVNLNIE
                     LQKVLNPQDGKKAEKVSRVYTLREGDRVMQIKNNYSLRWEKPGNSGIDGTGVFNGDMG
                     TVAEIDDEEMKVIVLFDDDRLVVYDYSILDELEPSFAVTVHKSQGSEFPVVVMPIIPG
                     PEVLMTRNLLYTAVTRAKEMVVLVGEEDVLLGMVGNKRETLRYSGLADKLKTFSEYAD
                     II"
     gene            complement(8226..8588)
                     /locus_tag="PLG72_06570"
     CDS             complement(8226..8588)
                     /locus_tag="PLG72_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80497.1"
                     /translation="MLYEAILCLTYILAIYGLLMLISGIAESIHCRVRGRRPRVRVIL
                     LVRDSEEHIEYIVRNAVKKELAARLLSDAKLIIADAGSSDNTYRLLEKLQKAYPCIEI
                     RRIDEMDKVLAEFASGCH"
BASE COUNT         1779 a         2429 c         1907 g         2554 t
ORIGIN      
        1 aatttgccgg tactgcccgt tgcggcatcg gttccgtcat tgaacaaatc cccgccaagg
       61 acgttgacat tgatgctgtc tgcaagcccg atctgataat atatctgttc tgtattgctg
      121 acatccacgg caaatgtccc atcgtcatta agcagtttct ggtctgtctt gaaaccggta
      181 aagatatagc gtccggcata tgccgagttg ccgagattga taagctgcgt cctgagctgt
      241 atgagctcgt tcttggttgc ctgcagatcc tccgggctct caatagcatc gctgcctacg
      301 ataagctccc tgactctctg gaatatgtct cctatgtcgc ttagcgtcga ctcggttata
      361 tcaagccatg acctggcatc tttcaggttc ctctggtact gctgtatctc cgccacatca
      421 gtacgtagct tcagcgccct cgcagcaacg accggatcat cggaaggcac ctgtatcttc
      481 tttccggtag caagctgcgc ctggtatttg tccatccttg tcaggttgtt gccgatgtaa
      541 ttaatcatat tattgatcag catattgttc gttatccgca ttacataaac ctcctgagca
      601 aatcacagaa tgaatcaagc caaaccttgc aaatactgct gccgaaattc ctgcttatac
      661 aaacaatccg ttgatcaggg tctcatatac ctcattcatc gtctgtatca tctttgccgc
      721 cgcattgtac gcgtgctgga atttcaccag gttggccatc tcttcgtcca tcgaaacgcc
      781 cgaattggaa agcctcctgt tttcgatctg gttgacgacg gccttctggt tcatactgaa
      841 atttcgggcc tgctgcgaat caatgcccaa tgtggtcaca agggacttca tgaagtcttc
      901 cggagcccct tctgaaaaca tgtccgaatt cctcctgaaa ctgagcagca cattcagcac
      961 ttcgatattc ccggcttcgt actgtgtgtc cgacgcggct atcaggttat agtcgccgag
     1021 tatatcagcg ctgacgcaga aattctttgc cgtgattttt tcatatgcgg catatattaa
     1081 agccggatcc gactcgccgt tcaggaattc gtccgacgac agcactttcc cgttttcgtc
     1141 ggtcatggta aagaagcgaa tgcctgtcaa atctgagtta tagccgtatc cagaggaatg
     1201 gcctgtgccg aattcacccg gatcgaattc cctgtcgccg tccaggtcga tatatccttc
     1261 attgaacgca cgggcaaaga cccgtacgaa ttcgttcagt ttctttatgt agtaaggcac
     1321 tcctttgtag tttggcgaaa caagcccgtt gtcaggattc acgctgcctt cgttgccgtc
     1381 gcggaggtcc agcaggcctc tcaattctcc gccttttacg ttgagcttgt tgccgtcggc
     1441 ccattttact tcatataggt tggggatgtc ctcctggttg accattttat catcaggcct
     1501 ttgctccagc gccagcttgc tgacgctgaa atggtccacc agcgccttgc cgctgactgt
     1561 gatgacaaaa tgcttgtcct ctcttccgtc aggcgtgtat cctacgacaa tttcgttggc
     1621 ttcgatgttg attatcttcg aaagcctgtc caccaggacg gtgcgctggt ccctcatgtc
     1681 atttgccgta ttgccgctca gttccgttat atatatctgc ctgttcagtt gctgtatctg
     1741 tacagcaagc gagttgatct cttccacctt ggtctgtatc ctgtagttta tgtcatattg
     1801 cagttcctcg agtttcgttg ccaggttgtt gaagaatttt gccagcgtaa cgccgttctg
     1861 cctcacaagc gacctgacgg cagcgctgct cggatccttc gacagttcct gcagtgaatc
     1921 gaagaagctg ctcatgatcg cagtgaaccc gctgtcggaa ggttcgttga acaggacctc
     1981 caggttcttg agtatctcat cttttacgct ccattcgccg tatgacgggt tctcgcccca
     2041 atacttgaaa tccaggtact cgtcacggat cctttccact cctataactt ccgctccagt
     2101 ccccagcatg cctgtcccgt cgagaagggc catgggcctg gaagccacct ggattgcctg
     2161 ctgcctcgaa tagcccggtg tgtttatatt gttcaggtta tgatttacga tatccaggtt
     2221 ccgctgggcg gcgaaaagcc cgcggaccgc cacattaagt ccgaaaaatg atccactcat
     2281 ctattatcac ctgcctgtca gacccattct gttcactacg gtctccatca tcgaatctat
     2341 tatgttcagc actctcgatg ctgcatcata tgcaaatttg aatttcagca tgttcgtcat
     2401 ttcttcatcc atcgaaaccc ccgctatcgc tgtccgctgc atatcgatgg cctgcaccag
     2461 gttctgctgg cttccggtga tgttcaccgc ttctgatgct ttgtttccaa cggtgagtat
     2521 gatgtactgg taataatcat cgatgctgac aagccccgtt ttgctcgtta tcagggcttt
     2581 gttcctcagg ttcgcgattt ccagcgctat ggtgttgtcc ccagccatgc ccgttttcga
     2641 tgccgctatt acagacaggt cggaaagatt ggggttaagc gtgatattgc ccatctgcag
     2701 cggtttagcc gggttgatgg ccacaaagat gtcttcgccg tattcgggcg ggtcctttat
     2761 gttcatgccg cttttgtgca ggttgttgat ttcattcagc atcacgctga tgagcgtatt
     2821 gagcttgttt ttgacgtctg aaactatatt catcgtgtct tcggaagtca cgccgccgga
     2881 tacaggtatt tcacccctgg attccataag gcctttcagt atgccgctct tgaccggcac
     2941 ttctatgtcc gtgcccgcca gtttggccac gtaaaagata tcgccgggat tgcgttcctc
     3001 cgcgtaaaga tccgtactga tacccctctg gaccaggaaa taccctccca gcgtgatatc
     3061 tacctggcca tcctgcattt cgataacatt cacatcggcc agccgtgtaa gcctgtctat
     3121 aagcttgttg cgctggtccc tgtaatcggc ggccgtatcg cccgaaacct cgttctttaa
     3181 tatcgttaca ttgagttcgg cgatttccct cgtgatctcg tttatctcgc cgatcctgac
     3241 ggcgatctcg ttgttcaggt cattctgcag cctgtccagc tgttcgccca ggtggtttac
     3301 atactggacc agcgcctcgc tcctctgcct gaccagtgca cgaaccgtca ggctgtcggg
     3361 ttccttggcc agttcctgcc atgcgtccca gaactggtta agtatgtttt gcaggccgga
     3421 ttccatcggc tcgcccaata tagcctgcag atcgatgaat gtcttctggc gtgtctccca
     3481 gtaaccaagg gcggagttct ccttcctgta catattgtcg aggaaggtgt tcctgatctg
     3541 cctgatctcc tggatatccg cgccaagccc gacctgcatg agcccgccgc gcgtcagcac
     3601 ggtattgaca ggcccgttct tgatcatggc ctgttgcctg acatatccgg gcgtattgac
     3661 gttagcgata ttatgacctg tcacatacag cccgcgctca ttgaccgtaa ggcccgaccg
     3721 ggctatttcg taactggcga agctcactcc agtatgccct cctgtaacct cagaatttta
     3781 catcgaaaaa gttccttttt cccggatcgt ttgactggcc ggatttgccg tatatgtccc
     3841 ccggagatcc gacatttgaa aggacattga ccgaaaaatt tatataatcg agagagttct
     3901 ttatcagcct ggcattcagg tcattcgaat ttctcagatc actgaagata ccggtcagct
     3961 ttttctggca ttcagccagt tcttttgact ggtccttcgg aagcactttt tcgagttccg
     4021 tgacagtgat gctgtcagcg tccatcccga tctcttccga aagcttttcg acgattttct
     4081 cgcggttccc ctccagcttg gcaagccgga ggatcattga ctgttcgacc tttgtgatgt
     4141 tctcaagttc ggtgaccttt ccctggacga tcacatccgt tttctcatga gaaagtttga
     4201 gcaatgcttc atatattgct gcttcattat caagtatctc tatcagcgtt ttcaccatat
     4261 cggtatccaa agtaccacca cctgaccatg ccgtttccgg aaaaaagaaa ggagcttttt
     4321 actcctccgg atcgtcatgc tttcctgtca aaaacactcc ttattacctt gtcggctatg
     4381 tcacgccctt ttacatcgta ctggcctgtc tcgttcctcc cggcgatatc gtcgaccttg
     4441 tcctgccgga tatcaggcac ttccctcaat gccctgaata ccgcctggaa atccttggcc
     4501 tcactggaga tcgacaggac atcctttctg gatgcgacac ttccggtact tccgactctg
     4561 tttactttct tttgtgaacc atatataccg gttatctttg gcacatctcc tggtatcctc
     4621 attttaacca ctcatcctta attatctgat ttcttttcga ttcaaatagt atatcggaac
     4681 aatgtatccg tttattaact ggcaaattct ttttatagcg aaattattta tccctttcat
     4741 attccttgtt cagatatctc agccccagcg accttctgtc cgcggtgccc ctgctgtcca
     4801 ggtccagctt catctggtct gcagccgatt tgaatcccga tgccaggatc ctttcacatt
     4861 ccgggcagta cctgcctgtc ttaatggctt tcccgcagtt ttcacattcc agcagcaaat
     4921 tgccgtcatc atttgctatt tccagccttc cttctctcag gaaccttttt atcatttcta
     4981 cgctgacttc cagttcggtt gagacctggg tcaggctggc gccgggattc tcataaaggt
     5041 atttctttat cttctggaaa tcctcttctt ccttttccct gcacgccgga cagataggcg
     5101 ctcctcctat gtaattgaaa attttaccgc attttctgca attcctgaca tctggcataa
     5161 aaacccctcc ctcatctact ttcttcctgt ggcaactact acggcgaaca ccttcacggc
     5221 acccgcctgc ttcagcaccc tgctgcactc ttccagtgta gagcctgtgg tcagtatgtc
     5281 gtcgaccagc aggatcgact taccctttac ttttttaggt gattttacag aaaaagcacc
     5341 tttgacgttc tggttcctct tctgtctgtc caggaggctc tgtgcctctg tataccgttc
     5401 tcttttcaac acttttgagc attccggcag tcttattatc ctgctcaggg ctttcgaaat
     5461 caggtacgcc tggttgtatc ccctgataaa ctccttgtgc ttgtgcagcg gtacgctcat
     5521 gaccatatcg tactggctga tattcgtcag ctttttcagc ttgtcggcga tcagcttcgc
     5581 ataggtcatg taatatccgg gcttgttgta aaatttgaac cggatcagcg attctttcac
     5641 cataccggtg taatcgaata cgctcaatgc tccgtcgcag tgacctgctt catcttcctg
     5701 gctcgtcctg ataattgcgc ttcctgagaa tggcagcttg ccgtagcatg aaccgcatat
     5761 atggataatt gcgttgtaat ccagcaattg ctggcagaag atgcatctgg gaggatatat
     5821 caggtttact atgctttcaa acatcttcaa ttgccgtcag cccgctttcg tcatattata
     5881 tcggcatatt ccgaaaaagt ttttagcttg tcggcaagac cggaataacg caaggtctct
     5941 cttttgtttc ctaccattcc gagaaggacg tcttcttcgc ctaccaggac gaccatctct
     6001 ttcgcccttg tcacggctgt gtagagcaaa ttccttgtca ttagtacttc cggcccggga
     6061 atgatcggca tgacgacaac aggaaattcc gagccctggc ttttgtgtac ggtgacggca
     6121 aatgaaggct ccagttcatc gaggatgctg taatcataaa caacgagcct gtcatcatcg
     6181 aaaaggacga ttaccttcat ttcttcgtca tctatctcgg cgaccgtccc catatcgccg
     6241 ttgaaaactc cggtcccgtc gatccctgaa ttgcccggtt tctcccagcg gaggctgtag
     6301 ttgttcttta tctgcatcac cctgtcgcct tccctgagag tatacaccct ggatactttc
     6361 tccgcctttt tcccgtcctg cggattcagg accttctgca gttcgatatt caggttgacc
     6421 acgcccgccg gccctttttt ggtcggagaa agcacctgga tatccttcat cggatcaagg
     6481 ccgtaagtat caggcagcct tctgacgcaa aggtctatga ccgtacgcag tatattttcc
     6541 tgtccgcttc ttggcaggaa gaaaaaatcc ttgtccttcg cgttgagtat cggggcttcg
     6601 cccctgttta tcctgtgcgc gttcactatg atcaggcttt cttccgcctg cctgaatatc
     6661 tcggtaagcc gaacggtctt tatgccgctg cagcttatta tatcatggag cacgctgccc
     6721 ggtcccacgg acgggagttg gtccgcatct cccacaagga caagcctcgc tccaggcgat
     6781 atcgctttca gaaggtgatg catcagaatt atgtccacca tggacatctc atctatgatc
     6841 accgtatccg cctccagcgg attcgcttca ttcctctgga ataccagttc gctttcatca
     6901 tcggcataac ctatttcaag aagcctgtga atggtcttcg cttcatagcc ggtcgcttcg
     6961 gtcatcctct tcgccgctct tccggtaggt gccgcaaggg cagccctgta tccgtactct
     7021 gacagcagcc gcaatataca tttgataata gtcgtctttc cggtgccggg gccgccggtt
     7081 atgaccatca cgccgttacg catcgcctcg cgcactgctt ccctttgctt ccggtcaagc
     7141 tgtattcctt ccctctcttc aaagtccctt atcttttcgt ccaggtcctc aaagtccctg
     7201 gcatggcttg tccgcgacaa actcaacagc cgtttgcata cgccggtttc cgcggtgtag
     7261 aatgcggaca ggtaaacatt gctgccatcc tcggtctttt cagtacatac ggccttgtca
     7321 aacacgaggg ataccagtgc gtcctcgata tcgacttcag gaatccccag caattgggat
     7381 gtgtacattt tcaccatgtt ttcgggcagg tatgtatgtc cgttggaggc cgcgcggctc
     7441 aatatgtacc tgatcccgct cctgatccgg aagagggagg cagggtcgac tccgagcctc
     7501 atcgctatcc tgtcggctgt tttgaatcct atcccgaaaa catcctcgca aagcctgtac
     7561 gggttctccc tgatcatctc aacggacttg ttcccatacg ctttgaatat cctgatgcag
     7621 accgaaggac tcaccccgta ttcctgcagg aacatcacca cgtcctgcaa tcccctgtgt
     7681 tcgttcagag cctgtccgat gtccattgcc ttggcaaggc ttatgccttt tatttcggat
     7741 aaacgctcag gttcattgct gataacatcc agggcggacg tgccgaaccg ttccactatt
     7801 tttctggcgg taacaggccc tactcccttt attacgccgg acgccaggta tttctctatc
     7861 gattcggcag tgtggggcag caccttttca tacatctcca ccttgaactg gtcgccgtag
     7921 tcgggatgct tcacccatct gccgtttgcc tttatggtct caccttcgtt tacaaacggc
     7981 atatatccta cggccgtgat gatcttcccg ccggaagcta tctcacatac cgtatagccg
     8041 tttgcttcgt tcttgtatat gatctcttcg ataacgcctt caaccaatgc cggatccatt
     8101 ttatccacgc ttcgaaacct gtgcacgtat gggtttccaa ttttttagtt attacttata
     8161 gtatattaca ctttcaccgg ggtgaaaaga gcgcaggggc ccggcagaaa cacaaaggca
     8221 gcgcatcagt ggcagcctga tgcaaattct gccaggactt tgtccatctc gtcgatcctg
     8281 cggatttcga tgcacggata tgccttctgc agcttttcca gcagcctgta tgtattgtcg
     8341 gaagaacccg catccgcgat gatgagtttc gcatcggaca gaagcctggc tgccagttct
     8401 ttttttactg cattccgtac tatatactcg atgtgctctt ctgagtcacg gacaagcagt
     8461 atgaccctta ccctggggcg gcgccctctt accctgcagt gtatcgattc ggcaataccg
     8521 gaaataagca tcagaagtcc atatattgcc aaaatgtagg tcaggcataa tatagcctcg
     8581 tacagcatca aaccgcctcc atataattcc aggttatata atatgcacca tatatcgaaa
     8641 tgttacctgg actctggtta atcgtatgg
//
LOCUS       DAOSCC010000098         8649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5329_length_8649_cov_4.078776, whole genome shotgun sequence.
ACCESSION   DAOSCC010000098 DAOSCC010000000
VERSION     DAOSCC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5329_length_8649_cov_4.078776"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /gene="glgP"
                     /locus_tag="PLG72_06575"
     CDS             <1..1998
                     /gene="glgP"
                     /locus_tag="PLG72_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ80498.1"
                     /translation="NAEQDRHITSRLYGGNHETRIQQEILLGIGGVRALEALGIRPTV
                     YHMNEGHSAFLCFELIRRSMIKYNIGFHEAMELVSPSLVFTIHTPVPAGIDVFPHDAM
                     DMYFTSFRESLGVSREEFLALGRDPGNPYGFNMAVLAMKMASGRNGVSKLHGAVTRRM
                     FSGLWPGVPEDEVPVTHVTNGIHTLTWLSPDLRKLFDKYLPEGWENRIYERSTWKFVE
                     NIPDEELWETHLSLKKSMAEYVNARIRSGQMSAWEMKQCMPIDPDALTIGFARRFATY
                     KRAALIFRDIDRIRKLFGRENAPIQIIFAGKAHPADKPAQDLIKYINDISGQEGFRGK
                     VVLLENYNMALARRLIQSVDVWLNNPRMPLEASGTSGQKAGLNGVLNFSVLDGWWREG
                     YNGQNGWAIGTEEIYPDESCQDKADSDSLYETLENSIIPLYYQRNEKGIPTGWVRMMK
                     ESIMSLAPYFSTHRMLQDYMAKLYVPAMRMVCSISGGDGADCQKIIQLAQWKQHVRSN
                     WHLVNIKRGGWRADGPGAGAAVGTAANGAESDEAGSGPYSGTDAVSGTGACSGTDADI
                     TSFSGWKTTLEAVVSLGELKPEDVSVELYYGIIGDEGEIINGMHLPMQLRGEAEGGTY
                     IYSADLCLTGGGEYGYQFRVFPRHPLLTDKFGLGLVKWAEH"
     gene            2155..3534
                     /locus_tag="PLG72_06580"
     CDS             2155..3534
                     /locus_tag="PLG72_06580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ80499.1"
                     /translation="MQKKAFTIFLAIVFILTCSVGVCFADNETTAPVTAGAGPAAQNN
                     ASGAGNTASGTEGKESRAEDMGRGAASMVPTPEISAPSALLMEAETGQILYEKNINAP
                     LHISAANKLMTILVAIENGNLSSYVTASSESVNTEGSTLSLAVGEKYLLGDLLSAVML
                     TSANDAAIAVAEHVSSGDIGKFVDLMNKTAEKLGMSDTHFSNPTGLYDESQYTTARDI
                     ALLVRYAIANPQFNTLFTSKVKLWSDKNKSSLLTSPFRLFWEYDGILGGKTGYNTREK
                     QTVISTASRSNLKLISIVLDAPEETMFTDTKTLLDYGFNNFRKSTLVSKNDVIKTVEF
                     EGHEVRLISQSDIMYVHPLGESYIKDFNVNVDLKTPLKKTVPAGSAVYVLNDDTEVRI
                     SLYPESEIVPVEDVKTRLQKLVMENKDIFLIVAILAAIEILLLITNVGKLIARLITYI
                     QRRDRKGSE"
     gene            complement(3802..4560)
                     /locus_tag="PLG72_06585"
     CDS             complement(3802..4560)
                     /locus_tag="PLG72_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOJ80500.1"
                     /translation="MMMQAFSARSKFTMAVILLAAAAVFVAVIGAFANAPAEAAEAEA
                     EKRTINVSGQSKVTASPDFATINLGVVTEDKDAKVAQKTNAQSMEKVIKAIKALGIAD
                     SDIQTSNYSIYPVYNYKQETGESVITAYRVNNSVNVTVRDLNKAGSVIDAAADSGVNI
                     SSSITFGLSNYEDYYNEALKNAVLAANRKANTIAGALGVKLKGAVTVNEGGGYSPLTN
                     YVVYSKSDEAVGASTPIQAGELEITAYVNIVYEY"
     gene            complement(4861..5853)
                     /locus_tag="PLG72_06590"
     CDS             complement(4861..5853)
                     /locus_tag="PLG72_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ80501.1"
                     /translation="MKKEGLYLGIDVGSVSTNLVTIDENGQITAKHYRRTGGRPIQSL
                     TDGLKVIAEQCGNAAIKGVGVTGSGRELAAVIAGADIIKNEITAHAVAAQAIVPGVRT
                     ILEIGGQDSKIILLKDGVVCDFAMNTVCAAGTGSFLDRQAARLGVPIDEFGDLALRSV
                     SPVRIAGRCAVFAESDMIHKQQCGHSIEDIVNGLCEALVRNYLNNLAKGKEIHGPVVF
                     QGGVAANKGIAAAFEKALGLKLTIPKEYDVMGAFGAALIARRKMKNEKDPATKFNGFD
                     AANSTYETRSFECSGCPGLCEVVELRMDGHVRARWGDRCGRWSSTAAESLKTAQ"
     gene            complement(5822..6979)
                     /locus_tag="PLG72_06595"
     CDS             complement(5822..6979)
                     /locus_tag="PLG72_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HOJ80502.1"
                     /translation="MKVTFPHLGNTYICVKSILDDIGIDYVIPPFNNKRALELGTRYS
                     PEMVCLPLKINIGNYIQAYEIGADTILMAGGCGPCRFGYYSQLHREILKDAGFEMEVI
                     TLEGTDIKDLVSRVRRVVPGGSELMRLPSVLLRAVEIAKRTDEVEMLAFRTRARECER
                     GATDRIYNGFREKALATKGTRNILELLDETEARLKSVRLDPNADPLRVGIVGEIYTGI
                     EHFASFDIQHRLGRLGVEADRKVTVSNWIIEHMIKKPLRLPRDMAFAKAAKPWLGAMI
                     GGHARETVGNTAIYAMNGYDGVIQIFPFSCMPEIVAESLLPSVERDLDIPVLTLIIDE
                     MTGEAGYMTRVEAFIDMLEKRRENREKRRENGTCKRGESGTYKKRRLVSWN"
     gene            complement(6951..8063)
                     /locus_tag="PLG72_06600"
     CDS             complement(6951..8063)
                     /locus_tag="PLG72_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ80503.1"
                     /translation="MCAKIGIPRSLFYYRFAPFWEIFFSALGAEAVFSDPTNKRILDD
                     GVRSCVDEACLPIKLFYGHVLNLKDRVDYILVPRFTSISKNEYICPEFGGLPDMIRHN
                     LKGLPSLIDTEVNMRNSARGGLEAAVEIGAMIGADRRKAAKALRLASQNFRKDSFYTE
                     FKKKVLKGENRVQADAGKVETIPEGACQKSENRPQTECQKGENRPRPKSQKSENCPHG
                     ECRKRLCVGVIGHPYTVYDRYISMDLLRKLADHGVESRTIESVDEADINRHASRLAKP
                     MFWNHGRMAYGAALHMAEGGEVDGFICLSSFGCGIDSFVNDLIERHVRRRFGIPLITM
                     TIDEHSGEAGFNTRLEAFLDMLQWRRQNEGHISASG"
     gene            complement(8414..>8649)
                     /locus_tag="PLG72_06605"
     CDS             complement(8414..>8649)
                     /locus_tag="PLG72_06605"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ80504.1"
                     /translation="GAKGVPEDMLRKAAQSAVCKINIDSDLRLAMTATIREYFAQHPE
                     DFDPRKYLGPARQAIKDLVKHKLIHVLGCAGKA"
BASE COUNT         1964 a         2323 c         2234 g         2128 t
ORIGIN      
        1 aatgccgagc aggacaggca tataacctcg cgattatacg gcggaaacca cgaaacgagg
       61 atacagcagg aaatactgct cggcataggg ggagtcaggg cactggaagc cctcggcatc
      121 aggccgacgg tctaccatat gaacgaaggc cattcggctt tcctgtgctt cgaacttatc
      181 aggcgcagca tgataaaata caatatcggt ttccatgaag ccatggagct tgtttctccg
      241 tcgctggtat tcacgatcca tacgccggtg ccggcaggta tagacgtgtt cccgcatgac
      301 gctatggata tgtattttac gtcgttcagg gaatccctgg gcgtcagccg ggaagaattc
      361 ctcgcccttg gccgggatcc gggcaacccc tacggtttca acatggctgt cctggctatg
      421 aaaatggctt cgggcaggaa cggcgtaagc aaactccacg gtgccgtgac aaggcgcatg
      481 ttcagcggcc tgtggccggg tgttccggag gatgaggtgc ctgtgacaca tgtcaccaac
      541 ggcatccata ctcttacatg gctttcccca gacctgcgga aactctttga caaatatctg
      601 ccggaaggat gggaaaacag gatatatgaa cgcagcacat ggaagtttgt cgaaaatata
      661 ccggatgagg aactctggga aacccatctg agtcttaaga aaagtatggc ggagtatgtg
      721 aacgccagga tacgttccgg ccaaatgtcc gcctgggaga tgaagcagtg catgcccatc
      781 gaccctgatg cgctcacgat aggatttgcc agaagatttg cgacatataa acgggcagcg
      841 ctgattttca gggacatcga ccggatccgg aaactgttcg gcagggaaaa cgccccgatc
      901 cagatcatat ttgccgggaa ggcccatccg gccgataaac cggcacagga tctgataaag
      961 tatataaacg atatttccgg acaggaaggc ttcaggggca aagtagtgct gcttgaaaac
     1021 tacaacatgg cgctggcgcg caggctcatc cagagtgtcg acgtgtggct caacaacccg
     1081 cggatgcccc tggaagcaag cggcaccagc ggccagaagg ccggcctcaa cggcgtcctc
     1141 aacttcagcg tgctggacgg ctggtggcgg gaaggttata acgggcaaaa cggctgggct
     1201 atcgggaccg aagaaatata cccggatgag tcttgccagg acaaagcaga cagcgactct
     1261 ttgtacgaaa cgcttgaaaa cagtataatc ccgctgtatt atcaaagaaa tgaaaagggt
     1321 atcccgaccg gctgggtcag gatgatgaag gaatcaataa tgtcgctggc gccatatttc
     1381 agcacccacc ggatgctgca ggattatatg gcgaaactgt acgtgcccgc catgcggatg
     1441 gtttgcagta tctccggcgg cgacggcgcg gactgtcaga agattataca gttggcacag
     1501 tggaagcagc atgtccgcag caattggcac cttgtgaata tcaaacgcgg cggatggcgg
     1561 gctgacggac ccggtgcagg tgccgctgtc ggtactgcgg caaacggcgc tgaaagtgac
     1621 gaggcgggtt ccggcccata ttccgggact gatgcggtgt ccggtaccgg ggcctgctcg
     1681 gggacagacg ccgacattac gtctttctcc ggctggaaaa ccacgctgga agcggtcgta
     1741 tcgctgggag agcttaagcc ggaggacgtg agtgtcgagc tgtattatgg tatcatagga
     1801 gatgaaggtg agataattaa tggcatgcac ctgccgatgc agttgcgcgg cgaagccgag
     1861 ggcggcacat acatatattc ggccgacctg tgtctcaccg gcggaggcga gtatggatac
     1921 cagttcaggg tattccctcg acacccgctg ttaacagaca aattcggact tggactcgta
     1981 aaatgggctg agcattgagc ggaaccagcc ggactttgga tacagggtcg actggaaatc
     2041 aactgagatc caccgaactg tcagtcggac tttgatgaaa aacagttgga ctttataagt
     2101 cggactttga ccggaaacaa ttccgaacgc ttatgtaact ccatggagga cgtaatgcaa
     2161 aagaaagctt tcacgatatt tcttgcaatt gtctttattc tgacgtgctc tgttggtgtg
     2221 tgctttgcag ataatgaaac cacagctccc gttactgcag gcgccggtcc ggctgctcag
     2281 aacaacgcct caggagccgg caacacagcc tccggtaccg aaggcaaaga atcacgtgct
     2341 gaagacatgg gccgcggcgc cgcaagcatg gtgccaactc ccgaaatcag cgcaccttcc
     2401 gccctgctta tggaggcgga gaccggacag atactttatg aaaagaacat caacgctcct
     2461 ctgcatatat cagctgccaa taagctcatg acaatacttg ttgcaataga aaacggcaat
     2521 ttgtcctcct atgtcacggc gagttccgag tcggtcaaca ccgaagggtc gacgctcagt
     2581 ctcgctgtcg gtgagaaata cctgctgggc gacctgctga gcgccgtaat gctgacttcc
     2641 gccaacgacg cggctatcgc ggtcgccgag catgtttctt ccggcgatat cggcaagttc
     2701 gtcgacctga tgaacaaaac agccgaaaag ctggggatga gcgatacgca tttctccaat
     2761 ccgaccggtc tgtatgatga aagccagtac acgacggcac gggacattgc gctgcttgtg
     2821 aggtatgcca tagcgaatcc gcagttcaat acgctgttca catccaaggt gaagctgtgg
     2881 agtgacaaga ataaatcgag cctgctgaca agtcctttca gactgttctg ggaatatgac
     2941 gggatcctgg gcggcaagac ggggtacaac accagggaaa agcagactgt aataagtact
     3001 gcgtcgcgtt cgaacctgaa gctgatcagc atagttctcg atgccccgga agaaacgatg
     3061 tttactgata ccaaaacgct cctggactat ggtttcaata atttccggaa gagtacgctg
     3121 gtaagcaaaa acgacgtcat aaagaccgtc gaatttgaag ggcacgaggt caggcttatc
     3181 agccagagtg acataatgta tgtccaccct ttgggcgaaa gctacatcaa ggacttcaac
     3241 gtcaatgtgg atctcaagac gccgctgaag aagacggtcc cggccggaag cgcagtttat
     3301 gtcctgaatg atgatactga agtgcgcatc agcctgtacc cggaaagcga gatcgttccg
     3361 gtggaagatg tgaaaaccag gttgcagaaa ctggttatgg agaataagga catattcctc
     3421 atagttgcga tactggccgc catagagata ctgctgctta taacgaatgt cggcaagctt
     3481 atcgccaggc tgatcacgta tatacagcgt cgtgacagga aaggttccga gtgaacaagg
     3541 ttatggaaag cattgaaagt ttggagtcat ggcagcaacg acagtatcag gcacccggtg
     3601 caggtcagca ttatcgcagt cctgatcggt taaagcgatc aaaaggccag gagtctgttt
     3661 cttcagcaga ccccaggcct ttttaagttt tgctcccttt ctgtgtcact tatccgtctc
     3721 atcaatactg acgatatttt tcatcattat ttgtatcaat aatgttacga tattatcggc
     3781 aaacatcgcc ggtttgcatc atcagtactc ataaacgatg ttcacatacg cggtgatttc
     3841 gagttctccc gcctgtatcg gagtcgatgc gccgactgct tcatcgctct tgctgtatac
     3901 tacataattg gtcagcgggc tgtatccgcc gccctcgttg accgttacgg cacccttcag
     3961 tttgacgcca agcgcaccgg cgatcgtatt tgccttcctg ttggctgcaa gcacggcatt
     4021 cttcagcgct tcattgtaat aatcctcgta attgctgagg ccgaaggtga tgctgctgct
     4081 tatgtttaca ccgctgtcgg ccgcagcgtc gatcacactg cctgctttgt tcaggtccct
     4141 tacagtaaca ttgacagaat tgttgacgcg gtaagccgtg ataacgctct cgccggtctc
     4201 ctgtttatag ttgtaaacag gatagatcga gtagtttgac gtctggatgt ccgaatcggc
     4261 gatacccagc gccttgatgg cctttatgac cttctccatc gactgcgcat tggtcttctg
     4321 ggcgactttg gcgtccttgt cctcggtcac gacgccgagg ttgatggtcg caaagtcagg
     4381 cgaagcggtg actttggact gtcccgagac atttatcgtc cttttttctg cttccgcttc
     4441 tgccgcttct gccggcgcat tggcaaaagc gcctatcact gcgacgaaaa cagctgcggc
     4501 ggcaagaagg atcaccgcca ttgtgaattt actcctggca gaaaacgcct gcatcatcat
     4561 atacacctcc aataacagca gacaaaattt ttatcccgtt gcggcgtttt atcttccggc
     4621 acgcctgcgg ttaatgacag tacgcctgta attattgaaa acgggacagt ctgcatgatt
     4681 ttcctttatt ggattagacg tcggtcacag gcagaagttc cggacagaca gaatgttttc
     4741 agggatatgt acagatcatg ttggaaacgt acagatcaga ctgaaaatgt attgctcggg
     4801 caaataagtg caggctggcg ggtaataagg ccagaccgga agtgcatgga gcggatatcg
     4861 ttactgtgcc gtcttcagcg actctgccgc agttgacgac catcttccgc acctgtcgcc
     4921 ccaccttgcc cgtacatggc cgtccatccg cagttcgacc acttcgcaca gtccggggca
     4981 tccgctgcac tcgaagcttc ttgtttcgta ggtactgttt gcggcatcaa atccattgaa
     5041 ttttgtcgcc ggatcctttt catttttcat ttttcgccgg gcgatgagcg cagccccgaa
     5101 tgcccccatt acatcgtatt ccttcgggat cgtcagcttc agcccaaggg ctttttcaaa
     5161 cgccgcggct atgcctttgt ttgcggctac accgccctgg aaaacgacgg ggccgtgtat
     5221 ttcctttccc tttgccaagt tgttgaggta gtttctgacc aaagcctcgc acaggccgtt
     5281 tacgatgtct tcgatgctgt gtccgcactg ctgtttatgt atcatgtccg actcggcaaa
     5341 aacagcgcat ctgccggcta tcctgacagg cgagacggat ctcagcgcca ggtcgccgaa
     5401 ctcgtcgatc gggactccga gccttgcggc ctgcctgtcg agaaaggatc cggtccccgc
     5461 cgcgcagacg gtgttcatgg caaaatcgca gaccacgccg tctttcagca gtattatctt
     5521 cgaatcctgc cctcctatct caaggatggt ccttacgccc gggactattg cctgggcggc
     5581 gaccgcatgg gccgttattt cgttctttat tatatccgct cctgctataa cagccgccag
     5641 ttcccgtccg cttcccgtga caccgacgcc ctttatcgcc gcattgccgc attgctccgc
     5701 gatcactttc agcccgtcgg tcagcgactg gatgggtctg ccgccggtgc gcctgtaatg
     5761 ttttgctgtt atttgcccgt tttcgtcgat cgtgaccagg ttcgtactga cagagcccac
     5821 gtcaattcca agatacaagc cttctttttt tatatgtccc actctcccct cttttgcatg
     5881 tcccgttctc tctccgtttt tcccggtttt ctcttcgctt ttccagcatg tcgataaatg
     5941 cttcgactct cgtcatgtac ccggcttcgc cggtcatttc gtcgattata agcgtcagca
     6001 ccggtatatc caggtccctt tcgaccgacg gcagcaggct ttccgccact atctccggca
     6061 tgcaggaaaa cgggaatatc tgtatcacgc cgtcgtaccc gttcattgca tatatggcgg
     6121 tgttgccgac ggtttcccgg gcatgtccgc ctatcatcgc tcccagccag ggttttgccg
     6181 cttttgcaaa tgccatgtcc ctgggcaggc ggagcggctt ttttatcatg tgttcgatta
     6241 tccagttgct caccgtcact ttcctgtcgg cttccacgcc cagtctgcca agcctgtgct
     6301 ggatgtcgaa gctggcaaag tgctcgatgc cggtgtatat ctcccctact atgccgactc
     6361 tcagcggatc cgcattggga tccaggcgga ccgacttcag cctcgcctct gtttcatcca
     6421 gcagttccag gatatttctc gttcctttcg ttgcgagggc tttttccctg aacccgttgt
     6481 atatcctgtc cgtggcgccg cgttcgcatt cgcgggccct ggtccggaaa gcaagcatct
     6541 cgacctcgtc tgtacgcttt gcgatttcca cggcacgcaa aaggacggac ggcagcctca
     6601 tcagttccga tccgccgggc acgaccctgc gtacccttga gaccaggtcc tttatgtccg
     6661 tgccctccag tgtgatgact tccatctcga agcctgcatc cttcagtatt tccctgtgga
     6721 gctgggaata gtatccgaac ctgcacgggc cgcagccgcc ggccataagg atcgtatcgg
     6781 cgccaatctc atacgcctga atatagttgc cgatatttat tttcagcggc aggcatacca
     6841 tttcaggaga gtatcttgtg ccaagttcca gcgccctctt gttgttgaag ggcgggatga
     6901 cgtaatcgat gccgatatcg tccagtatgg attttacgca gatatatgtg ttacccagat
     6961 gcggaaatgt gaccttcatt ttgccttctc cattgcagca tgtccaggaa agcttccagc
     7021 ctcgtattga acccggcctc tcctgaatgc tcgtctatcg tcatggttat gagcggaatg
     7081 ccaaacctgc gccttacgtg cctttcgatc agatcattaa cgaacgagtc tatgccgcac
     7141 ccaaagctcg aaaggcatat gaacccgtcg acttcgccgc cctcagccat gtggagcgct
     7201 gcgccataag ccatcctgcc gtggttccag aacatcggct tcgcgagcct ggacgcatgc
     7261 ctgtttatgt cggcctcatc caccgactct atcgtcctgg actcgacccc gtgatccgcc
     7321 agtttcctca gcaggtccat gctgatgtac ctgtcgtaca cggtgtacgg gtgccctatc
     7381 actccgacac aaagccgctt gcggcactcc ccatggggac agttctcact tttttgactt
     7441 ttcggccggg gacggttctc acctttttga cattccgttt ggggacggtt ttcacttttt
     7501 tgacaagccc cttccggaat ggtctcaact ttcccggcat ctgcctggac acggttttca
     7561 cctttcagca ccttcttttt gaactctgta taaaaactgt ccttccggaa gttttgcgat
     7621 gcaagccgga gggccttagc agcctttcgc ctgtcggcgc cgatcatcgc tccgatttca
     7681 actgctgctt ccaggccgcc cctggccgaa tttctcatgt tgacttccgt atcgatcaga
     7741 cttggcaacc ctttcagatt atgcctgatc atatccggga gcccgccgaa ttccgggcat
     7801 atgtattcgt tttttgatat gctcgtgaac ctcggcacca gtatataatc gaccctgtcc
     7861 ttcaggttca gaacatggcc gtagaagagc ttgataggca ggcaagcctc atcgacacag
     7921 gatctcacac cgtcatccag gatccgcttg ttggttggat cggagaatac ggcttcagca
     7981 ccgagagctg agaaaaatat ttcccagaac ggagcaaatc tgtagtaaaa caagcttctc
     8041 ggtatcccga tcttagcgca caaaagccca acactccctg atcataagat agttcttcct
     8101 ttatatccga aagattttct taattccatc tcagactatt atttacatac tcgtgaagtt
     8161 tattcaggat gtacaggatg ggcagaggat catatgcata acggcagaac aattatgtaa
     8221 gcggtaaaac gaacggttcg gcactcgatc cgacgccaga atgttaaaaa agtgagaagt
     8281 gtcccttccc gtaatgttga aaaggtgaga agtgtcccct cccgtaatgt tgaaaaggtg
     8341 agaactgtcc ccgtaaaaaa accggtatca ctgatgatac cggttttcgt caattgaaag
     8401 gaagcttttt taatcaggct ttgcctgcgc agccgagcac gtggatcagc ttgtgcttta
     8461 caaggtcctt gatggcctgt cttgcaggtc caaggtactt cctgggatca aagtcttcag
     8521 gatgctgcgc gaagtactcg cggatcgtag cggtcatggc gagtctcagg tcggagtcga
     8581 tgttgatctt gcagactgct gactgtgccg ccttcctgag catatcttcc ggaacgcctt
     8641 tcgcaccgg
//
LOCUS       DAOSCC010000099         8621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5351_length_8621_cov_5.051483, whole genome shotgun sequence.
ACCESSION   DAOSCC010000099 DAOSCC010000000
VERSION     DAOSCC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5351_length_8621_cov_5.051483"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLG72_06610"
     CDS             complement(<1..736)
                     /locus_tag="PLG72_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ80505.1"
                     /translation="MPRNENIRKVMVIGSGPIIIGQAAEFDYSGSQACKSLKEEGIEV
                     VLINSNPATIMTDGDTADRVYIEPITLDVVKKIIERERPDGILASFGGQTGLNMAVQL
                     ANDGILDRYGIELLGTSLMSIKKAEDRELFKETMQSINEKVPMSTIVTNLDDAVAFAR
                     KIGFPIIIRPAYTLGGTGGGIANNMEEFKYICGKGLKLSMIHQVLLEQSVAGWKEIEF
                     EVMRDGADNCIIICSMENLDPVGVHTG"
     gene            complement(813..>1545)
                     /locus_tag="PLG72_06615"
     CDS             complement(813..>1545)
                     /locus_tag="PLG72_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOJ80506.1"
                     /translation="RGIDTRALVKHIRSNGSMYGIISTECGNVDVLLERLRAGMQERR
                     SLVAEVTVKEPIHLEGQGKRIALLDLGTKRTLVRYLKEHGCDIYVLPASSAYEDVMAY
                     DPDGIIVSSGPGDPRDLPELQQLLARLIGRKPVFGVGLGHLLLGLALGGTVCKLKYGH
                     HGANIPVKDNTTGRCYITSQNHNYALNADINDDVEITQININDNTVEGFRHKKLPVSG
                     VQYCPGSADCPRDSAYIFEEFLNIL"
     assembly_gap    1546..1555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1556..1875)
                     /locus_tag="PLG72_06620"
     CDS             complement(<1556..1875)
                     /locus_tag="PLG72_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOJ80507.1"
                     /translation="MKSVLVLEDGTYYPGLGFGAKGETIGEVVFNTSMTGYQEIVTDP
                     SYRGQIVAMTYPLVGNCGFNREDSESGGAQVQGFIVKELCDSPNNWRCSIDAEEYFRM
                     NNIIG"
     gene            complement(1996..3192)
                     /locus_tag="PLG72_06625"
     CDS             complement(1996..3192)
                     /locus_tag="PLG72_06625"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOJ80508.1"
                     /translation="MDLREVMEMDSKYYMNTFGQRTPVCFEYGKGINLYSSDGRKYVD
                     LLGGIAVNALGHAHPALVEAVCSQAQKLIHCSSLYYIEAQAKLAKMLAESSCADRVFF
                     ANSGAEANEGAMKLARIYFKKKGMPEKYGIITLKDSFHGRTLATVAATGQEKYQKPYA
                     PLMPGFKHVQINDIEALKAGIDDKTCAVMMELVQGESGVHPLDAEYVRAVRSLCDEKG
                     LLLIIDEIQTGIGRTGKLFAYEHYGIEPDIFTLAKALAGGVPIGALCAKESVARAFEP
                     GDHGSTFGGNPLACSAGLAVLKTMLDEGLPANAEKMGRYFMDELKKLSAKHSVISEVR
                     GIGLMIGIQLAQDKAVALKGRLFERGYLVGSVGTSVIRLLPPLIITKDNIDPFISTLD
                     EVLASL"
     gene            complement(3356..4270)
                     /gene="argB"
                     /locus_tag="PLG72_06630"
     CDS             complement(3356..4270)
                     /gene="argB"
                     /locus_tag="PLG72_06630"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ80509.1"
                     /translation="MDQKMESIIRKAEILIEALPYMQKLHGKTVVIKYGGNAMISDEL
                     KNSVVEDITLLKYIGLNPVVVHGGGPDINSMLQRLNIKTRFVNGLRVTDMQTMEAAQM
                     VLVGKTNKEIVSLINQKGGKAIGICGIDGSLLECEQLRTVVDGVETDIGQVGRITKVN
                     SRMLELMAKDEYIPVVAPIGIGKDGRSYNINADTVAGEIAAALKAEKLMLLTDVEGVK
                     KSKDSPEVYYALTAAEVHRLIDEKVIEGGMIPKVLGCLEALDRGVGRTHIIDGRIPHC
                     ILLEIFTNQGIGTAIMKEKVPYYENEKI"
     gene            complement(4418..5368)
                     /gene="argC"
                     /locus_tag="PLG72_06635"
     CDS             complement(4418..5368)
                     /gene="argC"
                     /locus_tag="PLG72_06635"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ80510.1"
                     /translation="MVYKVFVDGQEGTTGLEINERLHARSDIELLKISPEKRKDINER
                     KKYINEADIVFLCLPDDAARESVSLVENENTRIIDASTAHRTNDSWTYGFPELSSGHR
                     EKIARSKRVAVPGCYATGFTALMYPLVREGIVPADYPVTCHAVSGYSGGGKKRIAEYA
                     SAAPDDEALRSPRFYALALKHKHLPEMQKVTGLSQPPLFTPIIGYNYRGMTVAIPLYS
                     RLLPEKNSAESICDFLYSYYKDQHFIKVYPYGSEGGIVDGFLSATGCNGTNMLEIYVA
                     GNEEHILLTARLDNLGKGASGAAVQCMNIMMGIDERTGLE"
     gene            5599..6711
                     /gene="ugpC"
                     /locus_tag="PLG72_06640"
     CDS             5599..6711
                     /gene="ugpC"
                     /locus_tag="PLG72_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOJ80511.1"
                     /translation="MASVKLKNVYKRYEGGVTAVSDFNLDIEDKEFVILVGPSGCGKT
                     TTLRMVAGLEEVTEGEIYIGDRLVNDVQPKDRDIAMVFQNYALYPHMTVFDNMAFGLK
                     LRKTPKDEIKKRVHEAAKILEIEHLLDRKPKALSGGQRQRVALGRAIVRDPKVFLMDE
                     PLSNLDAKLRVQMRLEITKLHQKLNTTFIYVTHDQTEAMTMGTRIVVMKDGFIQQVDT
                     PTALYDRPVNMFVAGFIGSPQMNFINATIEKRGDDIYLKFGSHEIKLPEAKAKKLENS
                     DYIGREVVMGIRPESIHDEEAYLQSMPDSIVTANVEVVEMLGSETLLHMYIDDVSVTA
                     RVDPRTKTRAGDVIKVAFDANRIHLFDKDTEKTIIN"
     gene            6897..7994
                     /locus_tag="PLG72_06645"
     CDS             6897..7994
                     /locus_tag="PLG72_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ80512.1"
                     /translation="MVVRLFQNLINQIRESVDGNFGIMDETGLILGCTDEQLTGTTHP
                     LIDEVTGDSRNEFEVRGWLFRKVFIRSRLEFIVFTDVPRDRGKALLAMFAINVSNLRT
                     YHEDKFDRGNFFKNVITDNILPGDIQVKAKELHIPFDTYRVVFLIKTEKSKDIHPHEV
                     ISGLFPKKQKDYVIALDDETLVLIKELKDADDQGEAEKTAKAIIDTLNTELMIKAQIG
                     IGSIVDNIRDIGRSFKEAQTALQIGSIFDSDKSIIDYNNLGIGRLIYQLPTTMCRLFL
                     KEVFKKGSFESLDQETLLTIQKFFENNLNVSETSRQLYVHRNTLVYRLDKIQKTTGLD
                     LRKFDDAITFKVSLLVKKYLDKAESMQGDII"
     gene            8333..>8621
                     /locus_tag="PLG72_06650"
     CDS             8333..>8621
                     /locus_tag="PLG72_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ80513.1"
                     /translation="MVELRNVSKTYPNGTVALRDINLKLDKGEFVFLVGPSGSGKSTL
                     VKLLLKEEDATEGEVYVNGYDVTSMTRQEIPYLRRSLGVVFQDFRLLPNKTV"
BASE COUNT         1971 a         2230 c         2196 g         2214 t
ORIGIN      
        1 cgccggtgtg cacgccgaca ggatcgaggt tttccatgct gcatatgatg atgcagttgt
       61 ccgcgccgtc gcgcatgact tcgaattcga tttccttcca ccccgcgacg ctctgctcga
      121 ggaggacctg gtggatcatg ctgagcttca ggcctttgcc gcagatgtat ttgaattctt
      181 ccatgttgtt ggctatcccg ccgcctgtgc cgccaagcgt gtaagccggc cttattatta
      241 tcgggaagcc gattttccgg gcaaaggcga ccgcatcgtc caggtttgtg acgatggtgc
      301 tcatggggac cttttcattg atactctgca tggtctcctt gaacagttcc ctgtcctcgg
      361 ccttctttat cgacatcagc gacgttccga gcagttctat tccatagcgg tcgagtatgc
      421 cgtcattggc gagctggacc gccatgttga ggccggtctg gcctccgaag gacgcaagta
      481 tcccgtccgg tctttcgcgt tctattattt ttttgaccac atccagggtg ataggctcta
      541 tgtagacgcg gtcggctgta tccccgtcag tcatgatggt ggcggggttg ctgttgataa
      601 ggacgacttc gatcccctct tctttgaggg atttgcaggc ctgtgagcct gaatagtcga
      661 attctgctgc ctgcccgatg attatcgggc ctgatcctat gaccattacc tttctgatgt
      721 tttcatttct tggcatattg acctccgtgc aatgactaaa ggcgtcttaa ataaggatgt
      781 taatgctttg gtatatatcc ggaattccct cttcaaagga tattcaggaa ttcttcaaaa
      841 atgtatgcgg aatcgcgggg acagtctgct gatccgggac agtactggac tccggatacg
      901 ggcagttttt tgtgtctgaa gccttccact gtgttgtcgt ttatatttat ctgcgttatt
      961 tcgacatcgt cgtttatgtc ggcgttcaga gcataattgt ggttttgcga cgtaatatag
     1021 catcttcccg tcgtgttatc ctttacgggg atgttcgctc cgtggtgtcc gtacttcagc
     1081 ttgcagacgg tacctcccag ggccagcccg agcagcagat gccccagtcc gacgccgaaa
     1141 accggcttcc tgccgatcag ccgtgcaagc agctgctgca gttccggcag atccctcgga
     1201 tcgccgggac cgctggatac gatgatcccg tccgggtcat acgccatcac atcttcatag
     1261 gcggaggatg cgggaagcac atatatatcg catccgtgct ccttaaggta tctgacgagc
     1321 gtcctttttg tcccgagatc caggagagct atccttttcc cctgtccttc caggtgaatc
     1381 ggttccttta cagtgacttc cgccaccagg ctgcggcgct cctgcatgcc ggccctgagc
     1441 ctctcaagca gtacgtccac attgccgcat tcggttgata ttatgccgta catgctgccg
     1501 ttgctgcgga tgtgcttgac cagcgcccgt gtgtcgatgc ccctgnnnnn nnnnncctat
     1561 gatgttgttc atcctgaagt attcttctgc atcgatggag caccgccagt tgttggggct
     1621 gtcgcaaagc tcctttacta tgaagccctg gacctgcgca ccgccggact cgctgtcctc
     1681 cctgttgaag ccgcagtttc cgaccagcgg atatgtcatc gccactatct gtcctctata
     1741 tgacggatct gttacgatct cctggtatcc cgtcatggat gtattgaaca caacttctcc
     1801 gatggtttct ccttttgccc caaagccgag cccggggtaa tacgtcccgt cttccagaac
     1861 caaaactgat ttcattgcca acgctccttg catgatcgat atctgtcggt ttgcccggcg
     1921 gcaggccggt atctctgtga taccggcctt cgccggatac atgctgtttc ggcagtggac
     1981 tgttagcagc aggcatcaca gcgatgccag tacttcatcc agtgtactga tgaacgggtc
     2041 gatattgtcc ttggttataa taagaggagg aagaagcctg atgacactgg ttcctacact
     2101 gcctacgaga taacctcttt cgaacagtct gccctttaat gcgaccgctt tgtcctgtgc
     2161 aagctggatg ccgatcataa ggccgatgcc gcgcacttcg gatatcacgg agtgctttgc
     2221 ggacagtttc ttcagttcgt ccataaagta tctgcccatt ttttctgcat tcgccggaag
     2281 gccctcgtcg agcatcgtct tcaggacggc caggccggcc gaacatgcca gcgggttccc
     2341 cccgaacgtg gagccgtgat ctccgggttc gaatgccctt gcgacggatt ccttcgcaca
     2401 cagcgctccg atggggacac ctcccgccag cgccttggca agcgtgaata tgtcaggttc
     2461 gatgccgtaa tgctcatatg caaagagttt tcccgtcctg ccgatacctg tctggatctc
     2521 gtcgattatg agcagcaggc ctttctcatc gcacaggctg cggacagccc ggacatactc
     2581 tgcatccagc ggatgcacgc cgctttcgcc ctgtacgagt tccatcatga ctgcacaggt
     2641 tttatcatcg atgcccgctt tcagcgcctc gatatcattt atctgcacat gtttgaatcc
     2701 cggcatcagg ggcgcgtagg gtttctggta tttttcctgc cccgtcgccg ccactgtggc
     2761 aagggtcctc ccgtggaacg aatccttgag agtgattatg ccgtactttt cgggcatgcc
     2821 cttctttttg aagtatatcc tggcgagctt catcgcgccc tcattggctt cggcgccgct
     2881 gttggcgaaa aagacgcggt ccgcgcagga tgactctgcc agcattttgg ccagctttgc
     2941 ctgggcttct atataataca ggctggagca gtgtatgagc ttctgtgcct gtgaacatac
     3001 cgcttcgaca agcgccggat gtgcgtggcc caaagcgttt acggcaatgc ctccgagtag
     3061 gtcgacatac ttgcggccgt cgctgctgta gaggttgatg cccttgccgt attcaaaaca
     3121 tacgggcgtg cgctgtccga aggtattcat gtagtatttc gagtccattt ccattacttc
     3181 gcgcagatcc atgggtccat ctcctttgca tatggaaaaa agctctattt gatatattat
     3241 tcataattat acgcacatat gcataaaaat gcaacataaa attactataa aaataaagaa
     3301 atgtccccgg aggacatttc gaaaagctga gtttggctgt aaaaccttat gctcttcata
     3361 tcttctcgtt ttcgtagtac ggcacctttt ccttcattat cgcggtgcct atgccctggt
     3421 tggtgaatat ctcgagcagt atgcagtggg ggatccttcc gtctattatg tgcgtcctgc
     3481 cgacgcccct gtccagggct tcgaggcagc cgagcacctt cgggatcatg ccgccttcta
     3541 tgaccttctc atctatcagc ctgtgcacct cggctgcagt cagggcatag tagacttccg
     3601 gactgtcctt tgatttcttg actccctcca cgtccgtaag cagcatcagc ttttcggctt
     3661 tcagcgcagc tgcgatctcg ccggccaccg tgtcggcgtt tatgttgtag ctgcggccgt
     3721 ctttaccgat gccgatgggg gcgacgaccg gtatgtactc gtctttggcc attagttcga
     3781 gcatcctgga attgactttc gtgatcctgc cgacctggcc gatatccgtc tccacgccgt
     3841 cgacgacggt cctgagctgt tcgcattcca gcaggctgcc gtctatcccg cagatgccta
     3901 tcgccttgcc gcctttctgg ttgatgaggg atacgatctc cttgttcgtc tttcccacga
     3961 ggaccatctg cgcggcttcc atcgtctgca tgtcggtgac ccggagcccg ttgacgaacc
     4021 gggtctttat attcagacgc tgcagcatgc tgtttatatc cggcccgccg ccatggacga
     4081 caacgggatt gagccctatg tatttgagca gcgtgatgtc ttccaccacg gagtttttaa
     4141 gctcgtcgct gatcatggca ttgccgccgt attttatgac gactgtcttc ccatgcagct
     4201 tttgcatata aggcagggct tcgatgagta tctcggcctt cctgattatc gattccattt
     4261 tctgatccaa agccatatac ctccatattc cgacatttga gccttatgcc ccggtttttc
     4321 ctgatccgtc cgttttgctg cgaacgatgc ccttgaacag cttgcgtgcc tggttcctta
     4381 tatgagcggc ttccaggata tgcttgcttc cttaatttta ttccagtcct gtacgttcat
     4441 caatacccat cattatgttc atgcactgga cggctgcgcc tgaagcgccc tttcccaggt
     4501 tgtccagcct tgccgtcagc aggatgtgct cttcattgcc tgcgacgtag atctccagca
     4561 tattcgtgcc gttgcagccg gtcgcgctga ggaagccgtc gacgatgccg ccttctgaac
     4621 cgtacgggta tacctttatg aaatgctggt ccttgtaata tgaataaagg aagtcacaga
     4681 tcgactccgc gctgttcttc tccggcagca gcctcgagta gagcgggatc gccaccgtca
     4741 tgcccctgta attatagcct atgatcggcg tgaagagggg cggctggctg aggcctgtaa
     4801 ctttctgcat ttccgggaga tgcttgtgtt tgagtgccag tgcataaaac cgcggacttc
     4861 taagcgcctc gtcgtcaggg gctgccgagg cgtactccgc tatacgtttt ttgccgcctc
     4921 cgctgtaacc ggagactgcg tggcatgtca ccgggtaatc tgccgggact atgccttccc
     4981 tgaccagcgg atacatcagt gctgtgaagc ctgtggcata gcagccgggc actgcgaccc
     5041 ttttggacct ggcgatcttt tccctgtgcc cgctgcttag ttcaggaaag ccgtaagtcc
     5101 atgagtcatt cgtcctgtgg gccgtacttg cgtcgatgat gcgcgtgttt tcgttctcta
     5161 ccagcgacac cgactccctt gccgcatcat ccggcaggca taaaaataca atatcggcct
     5221 cgtttatgta ttttttcctt tcattaatat ctttcctttt ctcgggcgat atcttcagca
     5281 gttcaatatc gctccttgcg tgcaggcgtt cgtttatctc gagccctgtg gtgccttcct
     5341 gcccgtccac gaataccttg taaaccattt tctgcctcct tgtgatccga tatgcgtctg
     5401 ctttaataat agcacattca ttggttcata attatacatt atgatgaata aaaattcaat
     5461 attatactgc aaatacatta tgcaaagtaa ctaaatagct ttttaatatt ttgttaaaaa
     5521 gtatactggt tagaaaccgg gtgacatgtt atattataac taacgttgct atgcttatat
     5581 aaatcaggag gggaaaaaat ggcaagtgta aagctgaaga acgtatacaa aagatacgaa
     5641 ggcggcgtta cggctgtcag cgacttcaac ctcgacatcg aggacaagga gttcgttatt
     5701 cttgtcgggc cgtctggctg tggtaagact actacactga gaatggtggc aggtctcgaa
     5761 gaagtaacgg aaggcgagat ctacatcggc gacagacttg ttaatgacgt tcagcccaag
     5821 gacagagaca tagcaatggt tttccagaac tacgctctgt atccgcacat gactgttttt
     5881 gacaacatgg catttggcct taaactcaga aagactccaa aagacgagat aaagaagaga
     5941 gttcatgaag cagcaaagat actcgagatc gagcacctgc tcgacaggaa gccgaaggct
     6001 ctctccggcg gtcagagaca gagggttgcg cttggccgtg ctatagtccg tgacccgaag
     6061 gtattcctca tggacgagcc tctttccaac ctcgacgcaa aactgagggt ccagatgaga
     6121 ctcgagatca ccaagttgca tcagaaactg aatacaacgt tcatatacgt tacgcacgac
     6181 cagacggaag ctatgacgat gggtaccagg atcgtcgtta tgaaggacgg cttcatccag
     6241 caggtcgaca cgcctacggc actttatgac aggccggtca acatgttcgt cgcaggcttc
     6301 atcggcagcc cgcagatgaa cttcatcaat gccacgatag aaaagcgcgg cgacgatatc
     6361 tacctcaaat tcggaagcca cgagatcaag ctgcctgaag cgaaggcaaa gaagctggag
     6421 aacagcgact atatcggaag agaagtcgtt atgggtatca ggcctgaaag catccatgac
     6481 gaagaggcat acctgcagag catgcctgac agcatagtca ctgcgaatgt cgaagtcgtc
     6541 gagatgctcg gctccgagac tctgctccac atgtacatcg acgacgtgag cgtcaccgca
     6601 agggtcgacc ccaggacgaa gaccagggcc ggcgatgtca tcaaggtagc gttcgatgcc
     6661 aacaggatcc acctgttcga caaggacacc gaaaagacga taatcaactg atcatatatg
     6721 aaacagaaga ggaagatcga caatcttcct cttttgtttt tcatcggctt atcatggccc
     6781 gcaggcagtt aaccatggca tgcggcaatc gaaaatctgt tattccatat ctttgcacaa
     6841 tatagtataa ttaaccctga taatgttttg tcactttgac gaatacagcg ggagggatag
     6901 ttgtgcggct gtttcagaat ctgataaatc aaattcggga gagtgtcgac ggcaatttcg
     6961 gcataatgga cgaaaccgga ctgatactcg gatgtaccga tgaacagctt accggcacca
     7021 cgcatccgct gatcgacgag gtgactggcg acagtaggaa cgaattcgag gtccgtggat
     7081 ggcttttccg gaaggtgttt atcaggagca ggctggagtt catagtcttc acggacgttc
     7141 ccagggaccg tggaaaagcg ctgctggcga tgtttgccat aaatgtgtca aatctgagga
     7201 cctatcacga agacaagttc gacaggggaa atttcttcaa aaacgtgatc accgacaata
     7261 tcctgcccgg tgatatacag gtaaaggcaa aggaactgca tatcccgttc gatacctaca
     7321 gggtggtttt cctgataaag acagagaaat cgaaggatat acatccgcat gaggtcatat
     7381 cgggtctttt ccccaaaaag cagaaggatt atgtgatagc gctggacgat gagacactgg
     7441 tccttataaa ggaactaaag gatgccgacg accagggtga agccgaaaag accgcgaaag
     7501 cgatcatcga tacgcttaac acggaactga tgataaaagc tcagataggc atagggtcca
     7561 tcgtggataa catcagggac atcgggcgct ctttcaagga ggcgcagaca gcactgcaga
     7621 taggcagcat tttcgacagc gacaaatcga tcatagatta caacaacctc ggcatcggaa
     7681 ggctgatcta ccagcttccc accacgatgt gcaggctgtt cctgaaggaa gtattcaaga
     7741 agggttcatt tgaatcgctg gaccaggaaa cgctgctgac tatacagaaa ttcttcgaaa
     7801 acaacctgaa tgtcagcgag acgtccaggc agctttatgt gcacaggaat acactggttt
     7861 acaggctgga caagatccag aagaccacag gccttgacct gaggaagttc gatgacgcaa
     7921 tcactttcaa ggtgtcgctg ctggtcaaga agtacctgga caaggccgag agcatgcagg
     7981 gcgatataat ctaaggaaag gggacactcc tgaaacaggt acggggacac acctctgcga
     8041 agaagtattc ctgtagggct gaggaatgtc cccgatggag acgaggaatg tcctaactga
     8101 actgggacac tccccgaagc aggtgcggag atactcctct gcgaagaagt attcctgcag
     8161 agacgaggaa tgtccccaaa atgtccgaac ttttgaaaca atcttgtaac acagaaaagc
     8221 aggaatttag cgcgttttgc agaaatgatt aacataatgt ataattatgg gcgtaccggc
     8281 gacatcgttc gacaagacgt gtcgtctgca tatatagccg aggtgcatag aattggttga
     8341 actgaggaat gtatcaaaga cctatcctaa cggtacggtc gcattgaggg atataaatct
     8401 gaaactggat aagggcgaat tcgttttcct ggtagggccg agcggatcgg gcaaatccac
     8461 gctcgtgaag ctgcttttaa aggaagagga cgccaccgag ggagaagtgt acgtaaacgg
     8521 gtacgatgtc acgtccatga cgaggcagga gataccgtac ctcagaagga gcctcggggt
     8581 cgtattccag gatttcaggc tgctgccgaa caagactgtc t
//
LOCUS       DAOSCC010000100         8542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5408_length_8542_cov_4.507482, whole genome shotgun sequence.
ACCESSION   DAOSCC010000100 DAOSCC010000000
VERSION     DAOSCC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5408_length_8542_cov_4.507482"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..1188)
                     /locus_tag="PLG72_06655"
     CDS             complement(331..1188)
                     /locus_tag="PLG72_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80514.1"
                     /translation="MPKMFPRTEVGGVSLSRMIIGTNWLLGWSHRSPAADNFIKSFHS
                     SKEAMSPLLETFLGHGVDTIMGPISSQPLMLEAIRHAEDRTGKKMIMIDTPIINVKSD
                     SLSRKEAYETIKRSAGIGSKFCLLHHSSVEQLVRKDSETIERLDEYTKMIREQGMIPG
                     LSAHMPELVVYSDKNGYDVETYIQIYNCMGFLMQVEVEYIAKVIQNAKKPVITIKPMA
                     AGRVTPFVGLTFVWNTIRPCDMVTVGCMTEQEADEDIEISLAAIEHRFPGMGQRSSPN
                     PDQAAFANR"
     gene            complement(1286..1933)
                     /locus_tag="PLG72_06660"
     CDS             complement(1286..1933)
                     /locus_tag="PLG72_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ80515.1"
                     /translation="MNNTGHGETLLKGVSLMKNAAIRIQRDKVYWFDPFRIDGEPRDA
                     DFIFISHDHWDHLSPEDIRKVIKNDTVLIAPESSAALLEGEGWNVTAVQPSKSYEVGG
                     VSFITVPMYNVGKKFHEKSENCVGYVVRIDGVDYYFAGDTDLVPEMESIKADVAFLPV
                     GGTYTMDASEAARAANIIKPAVAVPIHYADVVGTVEDGEAFIRGLDKPIKGVLLK"
     gene            complement(2105..4015)
                     /locus_tag="PLG72_06665"
     CDS             complement(2105..4015)
                     /locus_tag="PLG72_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOJ80516.1"
                     /translation="MAKKFGQASGKRLVYRDGHRIVIKSYYIDLLAVLIFSFGLSVLG
                     IMLSDVVDLMNIARYVRSPLLFLLNMLPLTLLMLLIYHISSRHWVAFGLGGGIYIIAH
                     IVNRFMMELREEPFLPADILLGTEAATVVRITELPFDRITVLSLVFWIVFTVLLLIFV
                     RSDKLKWPVRAAGAVAVIVLFMMSYIGIYKKTVLYDRFKVDGSIYSKVNLYKSRGFMY
                     SFLVRTGAYRSIKPEGYEKELAKQELAKYNEPGRPVVSGKMPNIIAVMGEAFYDIDRI
                     PGIEFNEGYDPLSNLNRIREKAYFGRIVTNVFGGGTANTEFSFLTGHSMPVMPELSSP
                     YSYYIRHDTFSLARVLEKYGYATMAFHPGESWFYNRVNVYRFFGFDSIYFKKDMDLEN
                     VEINHGYISDQDTAEFILEKFREQLEREPGRPFFQFTVDIDNHGPYSKKDIGYPQILK
                     KSKGMDESTYNILNNYLNGLMRCDKALGWLADSIMEMDEPIIFVYFADHLPWLGENDV
                     GYKALGFDIDPDGTLEEYLNKYETPYFILINKAAKELLTENGIQVKRGQGPQISVNYL
                     ATELLEAAGLDGGSYFNYLSELKKEIPVISSRFIKEKEQFTEEPSQDTKDMLETYGML
                     QYYMLTDKEAAQ"
     gene            complement(4313..5188)
                     /locus_tag="PLG72_06670"
     CDS             complement(4313..5188)
                     /locus_tag="PLG72_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80517.1"
                     /translation="MSRTYAEDLDGDGKAEDIGLVYERYKSDYGKGDLVLSVNGSKTA
                     VVEGYGTDMFTRPYRTIMEMPEVRYLAEQDGKSKALLAVYTWATNGVGSTGVVNAYRY
                     VNGDIREVEIIDAERVLEYKDDNIVNVSYPAFGRSFDLKLDEDFIGFYYKDEETFIQR
                     LDGYGPHPLRYHVNDFNGDGRDDLCCVSYLLDYPKVLCTEYIYYEYEDGAIKPVQVYA
                     CSSDIDEKMPYLKGYIIDRLNFKGYLDIGNNGIEDGNLQPYQECTPDEINSAVRELLK
                     DGILVRKGDRLYVCY"
     gene            5947..7686
                     /gene="metG"
                     /locus_tag="PLG72_06675"
     CDS             5947..7686
                     /gene="metG"
                     /locus_tag="PLG72_06675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ80518.1"
                     /translation="MNILIGNAWPYANGSLHIGHIACLLPGDVLARYHRAKGDTVMFV
                     SGSDCHGTPVSIRARQEGKTPQQISDRYHEEFVQCFKKLGFSYDLYSKTSSPEHKEFV
                     REYHRKLYEGGYVYEKTTLQTYCETCGQFLPDRFVRGICPNCGEKARGDQCEACGSVL
                     EPESLLEPECTVCGNTPELKPTTHLYLALTKMRDKLENYVESHKDWRKNAYEMSKRYL
                     DEGLRDRSLTRDLDWGIDVPKEGYENKKIYIWAENVLGYVSMCCTLCRQKGLDFFEFW
                     NTSRQYYAHGKDNIPFHTIILPSLLLGRTDCALKLPDVIISSEYMTLEGKKISTSNNW
                     AVWAKDMLERYDPDSIRYFLIANGPEQRDTDFTWTEFVSSHNGELLGAYGNFVNRSLK
                     FIEKFFDRTVPGNPSAEPEADVTARSDAGTGSVTAPGRPSGDIGSIYASATDSRSVEM
                     LKELQALYPSVGTQIEHGCFKAALETIFAYIRKANKYFDEQQPWITVKSDIQKCGETM
                     NTCVQIIANLSVLLEPFLPFSSEKTKNMLSMDCAWWHPVGIPAGLKLGRVDILFDRID
                     KKAIDEELERLRK"
     gene            7925..>8542
                     /locus_tag="PLG72_06680"
     CDS             7925..>8542
                     /locus_tag="PLG72_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOJ80519.1"
                     /translation="MILIPFPQKLEESQGTFRISGMSLIFLDTSCDYTDFEAALALRD
                     EIQRSAGAKPAITRGKYSERTNSGKDNAANPAVPGTIRPAGSDEAYPARSDAAYPDNM
                     IVLRKSIRSGAAKSGTGKKSVLSPVQAPDAGTATDSPSQQSYMLTVNRDAIVITGNGS
                     SGLYYGIQTLRQLIRNFGAEIPCLRIEDSPSLQYRGFYHDITRGKV"
BASE COUNT         2100 a         2318 c         2018 g         2106 t
ORIGIN      
        1 aaaatatcga ggaacagcct gaacttctcc tccgccatct ccttttccct gccctgcagc
       61 atcccgttgg catacggtat gacgctgaat tcggtacacc cgaacaaaaa ctcgtggctg
      121 acctgtttta tggctacctt ctggtttttg acaggtgtcc cgtccttctt cataatcatt
      181 accttcctgg agcccttccg atggtttagt tgatcatttc gtacatccgc cataaccgac
      241 cctcctgaca atatagtggt gtattctgcg aatcattgtg atatatcccg gatgattggc
      301 ttgtatttgc gatatgcagg ctttcttgca tcatctgttg gcgaaagccg cctggtcggg
      361 attcgggctc gaacgctgcc ccatcccggg aaatctgtgt tctatggcag ccagggatat
      421 ctcgatgtcc tcgtcggctt cctgctccgt catgcatcct acggtgacca tatcgcacgg
      481 ccggatggta ttccacacga acgtcaggcc gacgaacggc gtcaccctgc ccgcagccat
      541 cggcttgata gtgatgacag gctttttcgc attctgtatc acctttgcga tatactcgac
      601 ctcgacctgc atcaggaaac ccatacagtt gtatatctgt atgtatgtct caacatcata
      661 gccgtttttg tcggagtata cgaccagttc cggcatgtgg gctgaaaggc ccggtatcat
      721 gccctgctcc ctgatcatct tcgtatactc gtcgagcctt tctatcgtct cggaatcctt
      781 ccttacaagc tgttcaacgc ttgaatggtg cagcaggcag aatttcgacc cgatcccggc
      841 gctgcgcttt atcgtttcat aggcttcctt tcttgacagg gaatcactct ttacattgat
      901 tatcggagtg tctatcatga tcatcttttt gccggtcctg tcttcagcat gccttatagc
      961 ttccagcatc aagggctggc tgcttatcgg gcccattatc gtgtcgacgc catgccctaa
     1021 gaatgtttcc agcagagggg acatcgcctc ttttgatgaa tggaaagact ttatgaaatt
     1081 gtcagcggca ggactcctgt ggctccagcc caacagccag ttggtcccta tgatcatcct
     1141 cgaaagcgag acgccgccca cttcagtcct cgggaacatt tttggcattt atggaccccc
     1201 atttcgatcg taattattac tgccgaaaac tgctgcaaca gatcaccgac aacaggcaaa
     1261 tcccattgaa acagggcggc ttcggctatt tgagcaagac accctttatc ggtttgtcca
     1321 gaccgcggat aaaagcttcc ccgtcctcta cggtgccgac cacatcagca tagtgtatag
     1381 gcactgcgac agccggcttt ataatattgg cagcccttgc cgcttcgctt gcatccatgg
     1441 tataagtgcc gccaacaggc aggaacgcca cgtccgcttt tatcgactcc atttcaggaa
     1501 ccaggtccgt gtctccggca aaatagtaat cgacaccgtc gatcctcacg acataaccga
     1561 cacagttttc gctcttttcg tggaacttct tgcccacatt gtacatcggg accgtaataa
     1621 agctcacccc gcctacctcg tagctttttg acggctgtac ggctgtcacg ttccagcctt
     1681 ccccttcaag caatgcggca ctgctttcag gggctatcag gaccgtatca tttttgatga
     1741 ccttcctgat atcttcgggt gaaagatggt cccagtggtc atgagaaatg aaaatgaaat
     1801 ctgcgtccct cggttcccca tcgatcctga agggatcaaa ccagtatact ttgtcccgct
     1861 gtatgcggat cgccgcattt ttcatcaggc tgacgccttt cagcaaggtc tctccatgtc
     1921 cagtgttgtt cacggatatc gcctccttca tattccactt catatctcaa ttatatcacc
     1981 tttatcagaa gaattttgcg gttgcagaaa aaattcccca catcagcggt gtttcaacga
     2041 ggcgccaggt atataaagcc tcgttatcgc atcgttcata aaatttacga tccccggaaa
     2101 tttttcactg ggccgcttcc ttgtccgtga gcatatagta ctggagcatg ccgtatgttt
     2161 cgagcatgtc cttcgtatcc tgcgaaggct cttcagtgaa ctgttccttt tcctttatga
     2221 aacggctgct tatgaccggg atctcctttt tcagttccga caggtagttg aagtaactgc
     2281 ctccgtccag gcctgcagcc tcaaggagtt ccgtggcaag ataattgacg ctgatctgcg
     2341 gcccctggcc tcttttgacc tgtatcccgt tttcggtcag caattctttc gcggctttat
     2401 ttatcaatat gaaatatggc gtttcatact tgttcagata ctcctccagg gtcccgtccg
     2461 gatcgatatc gaagcccagc gctttgtacc ctacgtcatt ttcgcccagc cacggcaggt
     2521 ggtctgcgaa atatacgaaa attatcggct catccatctc cattatcgag tcggcaagcc
     2581 atccgagggc cttgtcgcag cgcatgagcc cgttcaggta attgttcagg atattgtacg
     2641 tggactcatc catacctttg ctctttttca ggatctgtgg gtaaccgatg tctttcttcg
     2701 agtaggggcc atggttgtcg atgtcgacag tgaactggaa aaacggtctg ccaggctccc
     2761 tttcaagctg ctccctgaat ttctccagta tgaactccgc agtatcctga tcggatatat
     2821 acccgtggtt tatctcgaca ttctccaggt ccatgtcttt cttgaaatag atcgagtcaa
     2881 atccgaaaaa ccggtatacg ttgaccctgt tatagaacca tgattcgccg gggtggaacg
     2941 ccatggtggc atatccgtac ttttcaagca ccctcgccag tgaaaacgtg tcatgcctta
     3001 tataatacga gtacgggctg gacagttcag gcatgacggg catggaatgg ccggtaagga
     3061 atgagaattc cgtatttgca gtgccgcctc cgaagacgtt cgtaactatc cttccgaagt
     3121 atgccttctc cctgatccgg ttgaggttgg aaagcgggtc gtacccttcg ttgaactcga
     3181 tgccggggat cctgtcgatg tcatagaagg cctcccccat aacagctatg atattaggca
     3241 ttttaccgct gaccaccggc cggccgggtt cgttgtattt ggccagttcc tgtttcgcca
     3301 gttccttctc atagccttca ggcttgatgg accggtacgc accggtacgc accaggaacg
     3361 agtacatgaa tcccctggac ttgtagaggt tgaccttgga atagatggac ccgtccacct
     3421 taaaacggtc atacaataca gtctttttgt aaatgccgat atatgacatc atgaacagaa
     3481 cgataacggc aactgctccc gcagctctca caggccattt cagcttatcg gacctgacga
     3541 atatgagtaa aagcacggtg aatacgatcc agaagacaag gctcagcacc gttatcctgt
     3601 caaaaggcag ttcggttatc ctgactaccg tggctgcctc agtccccagc agtatgtccg
     3661 ccggaaggaa cggctcctcc ctcagttcca tcatgaaacg gttgactata tgggctatga
     3721 tgtatatccc gccgccaagg ccaaatgcca cccaatgcct tgaacttata tgatagatca
     3781 gcagcatcag cagcgtcagc ggcagcatgt tcagcaggaa cagcagcgga gaccggacat
     3841 acctggcaat gttcataagg tccaccacgt ccgaaagcat tatgcctaaa acagataagc
     3901 cgaatgaaaa aataaggacc gcaagcagat ctatgtaata gcttttaata acgatcctgt
     3961 gtccatccct gtaaaccagc cgctttcccg atgcctgtcc aaacttcttc gccacctgtc
     4021 aaagacctcc taagactgta tactttacta tttttagcat aagtgtctgt tgccatactt
     4081 ttggatttct gtcacagaaa tcgataacac caccaaatta tatcactttt agttaagctt
     4141 tgccgccagg atgccgcctg ccgtcaacgc ggcgggccac agcataaaat ttctttgctg
     4201 tccattcttt taccaaacat atcatatact gcttttacgt ataaatctat aaccgctcgt
     4261 cttcttaaat cgctttttcg tccactcgct ttttgcgata tccgtcattt ccttagtaac
     4321 acacatacag cctgtcgcct ttgcgcacaa gtatcccgtc cttcaggagt tctcttaccg
     4381 ccgaatttat ttcatctggt gtgcactcct ggtaaggctg taagttcccg tcctcaatac
     4441 cgttgttccc gatgtccaga tagcctttga aattgagccg gtcaatgatg taccccttaa
     4501 ggtacggcat cttctcatca atatcggacg agcaggcata cacctggaca ggttttattg
     4561 ctccgtcttc gtactcatag tagatatact ccgtacacaa cactttcgga tagtcaagca
     4621 ggtaagaaac acagcaaaga tcgtcccggc cgtctccgtt aaaatcgttt acatgatacc
     4681 ggagcggatg agggccgtaa ccatccagcc tctgtataaa cgtttcctca tccttgtagt
     4741 aaaatcctat aaagtcctca tcgagtttga gatcgaaact tctgccaaat gccggataac
     4801 tgacgtttac gatattatcg tccttgtatt ccaggactct ttctgcgtcg atgatttcga
     4861 cttcccttat atcgccgttt acatacctgt aggcattcac aactccggtg gatccgaccc
     4921 cgttggtagc ccatgtgtaa accgccagta acgccttact tttcccgtcc tgttcagcaa
     4981 ggtatctcac ttcgggcatt tccatgatcg tgcggtacgg ccttgtgaac atatcggtgc
     5041 catagccttc cactaccgcg gttttgctgc catttacgct gagaaccagg tctcctttcc
     5101 catagtcgct tttatatctc tcatagacaa gcccgatatc ctcggctttc ccgtcgccat
     5161 ccaggtcttc ggcgtatgtt ctggacatta cgcgtatctt ttccccgtca atatcaacgg
     5221 cattattgcc ggtatcaaaa ataagacctg atgaccttac tttaatctat ggacggttta
     5281 acaaagtatg aagttctcat ctttgcatat tcataccaca gtaaataaaa tgcaccttga
     5341 catgcgtcct tttacatatt actataaatg gaacaacaga acacggaaaa tcagcgatga
     5401 agagaagagt aagtcgttgg cggccattac agagagctcc gggaaaacca tgctgagacg
     5461 gagtatggca cggatgaccg aagatggtcc tggagctgtt cgcggcgaac cggctttcaa
     5521 agccgctgag ccgtcgacgg gcgcgcccgt taccgcgccg gggaatggca tacggccgcc
     5581 tgtgcgatgc gtacctgtac gacacgcacc tgtgcgatac gtataagttc ctgctgaggc
     5641 cgaagctgcg aggtttcggt gaaccggggt ggtaccgcgg agtatgatgc tctcgtcccc
     5701 aggatatgtc ctgggggcgg gggtttttta tttatatcgg ttcaaatgcc agtcgatcgg
     5761 caattgggca gcagtttagg cgaatgcagg acaaatgccg acaaggcaga aagaacaacg
     5821 acggcaaaat caccagaaag tgcccgggca aagccagata cccgtgacgg taccgcacca
     5881 agatgtatac aggtcagaat ggcacagaac aagatgaaca taaaaacgtg aaaggaagtg
     5941 gagcatatga acatccttat cggaaacgca tggccgtatg ccaatggctc gctgcatatc
     6001 ggccatatag catgcctgct gccgggcgac gtactggcaa ggtaccacag ggcaaaaggt
     6061 gatacggtaa tgttcgtatc cggcagcgac tgccacggca cgccggtctc gatcagggcc
     6121 aggcaggaag gcaaaacgcc gcagcagatc agtgacaggt accacgagga attcgtacag
     6181 tgttttaaaa agcttgggtt ctcctacgat ctctactcaa agacgtcaag cccggagcat
     6241 aaggagttcg taagggagta ccacaggaaa ctgtatgagg gcggctatgt ctatgaaaaa
     6301 accactttgc agacatattg cgaaacctgc ggccagttcc tgccggaccg ctttgtcagg
     6361 ggcatctgcc ccaactgcgg cgaaaaggcc cgcggcgacc agtgtgaagc ctgcggcagc
     6421 gtgcttgaac cggaaagcct tctggaaccc gaatgcacag tttgcggcaa tacgcctgag
     6481 cttaagccca ccacgcacct ttacctggcg ctgaccaaaa tgagggacaa actcgaaaac
     6541 tatgtagaaa gccataaaga ctggcgcaaa aacgcatacg aaatgtcaaa acggtacctg
     6601 gacgaagggc tgagggacag aagcctgacc agggaccttg actggggcat cgacgtgccg
     6661 aaggaaggct atgaaaacaa gaaaatatat atctgggccg agaatgtgct cggctatgtt
     6721 tccatgtgct gcaccctgtg ccggcagaaa gggctggact ttttcgaatt ctggaacacc
     6781 agccgccagt attatgccca tggcaaggac aacataccgt tccataccat catactgccg
     6841 tcgctgcttc tcggccgcac cgactgcgcg ctgaaactcc cggatgtgat catatccagc
     6901 gaatacatga ccctggaagg aaagaagatc tccacaagca acaactgggc tgtatgggca
     6961 aaagatatgc tggaacgcta cgatcctgat tccataagat atttcctcat agccaacggc
     7021 cctgaacaga gggacaccga cttcacatgg accgagttcg tcagcagcca caacggagaa
     7081 ctgcttggag catatggcaa cttcgtgaac cgaagcctga aattcataga gaagtttttt
     7141 gacaggactg ttcctggcaa tccgtcggca gagcctgaag cagatgtcac tgcgcgcagt
     7201 gacgccggta ccggctccgt gaccgcaccc ggcagaccgt ctggcgatat cggcagcatt
     7261 tatgcatctg ccacagacag ccggtcggtt gaaatgctga aagaactgca agccctctac
     7321 ccgtccgtcg gcacacagat cgaacatggg tgtttcaagg cagcgctgga aacgatattc
     7381 gcatatatcc gcaaagccaa caaatacttt gatgaacagc agccatggat caccgtaaaa
     7441 agcgacatcc aaaaatgcgg cgaaacaatg aacacctgcg tacagatcat agccaatctc
     7501 tcagtgctgc tggaaccctt cctgcccttc tcatcggaga agacaaaaaa catgctctca
     7561 atggactgcg cctggtggca cccggtcggc ataccggcag gactgaagct tggacgggtg
     7621 gatatcctgt ttgacaggat cgacaaaaag gccatagatg aggaacttga aaggcttcgc
     7681 aagtgacccg ccatactgcg gcccaggctg agcggcatgc aagtacgggt ccgtatgcga
     7741 tcaggtcagg ccatttaccc tggtctgcct ggatcgcata caggcattta tacggacctg
     7801 cctgcagcag tctgatgtat cggcagcagt tcgcctcaag cggaggaaag cagtcaatcc
     7861 gggcttgact gcttaaacgg caggctataa cattatatta gcatcccaga atggagggtc
     7921 cggtatgata ttgatccctt tcccgcaaaa actggaagaa tcgcagggca cattccgaat
     7981 ctcaggcatg tccctgattt ttcttgacac ctcatgcgat tatactgact tcgaagccgc
     8041 actggcactc agggacgaga tccagagatc ggcgggagca aagccggcca tcacacgggg
     8101 gaaatattcc gaaagaacaa attccgggaa ggacaatgca gcaaatcctg ccgtgccggg
     8161 cacgatccgc cctgccggat ctgacgaagc ctatcccgcc aggtcggacg cagcctaccc
     8221 ggataacatg atagtgctga ggaaatccat ccgttccggt gctgcaaaat ccggtaccgg
     8281 gaaaaaatct gtcctctccc cggtccaggc gcctgatgcc ggtaccgcaa ccgacagccc
     8341 atctcagcag tcttatatgc tgaccgttaa cagggatgcc atagttatca ccggcaacgg
     8401 cagcagcggc ctgtattacg gcatacagac cctcaggcag cttatcagga acttcggcgc
     8461 cgagatcccg tgcctcagga tagaggacag cccttccctg caatacaggg gattctacca
     8521 tgacataacc cggggaaagg ta
//
LOCUS       DAOSCC010000101         8470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5457_length_8470_cov_3.521212, whole genome shotgun sequence.
ACCESSION   DAOSCC010000101 DAOSCC010000000
VERSION     DAOSCC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_8470_cov_3.521212"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLG72_06685"
     CDS             complement(<1..428)
                     /locus_tag="PLG72_06685"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ80520.1"
                     /translation="MKISVAATPAGNGTWNRPAAKGHSRRRDTFAGKIRDIPEPLQQS
                     KKRFGADSAKLKEGEIFMLKIIDMRQGKDSELFEKLADRESVGMQDVVDKVREIIENV
                     RKNGDRAVIEYTKKYDEVDLDASSIRVSGREIEEAYDSIDP"
     gene            complement(394..1056)
                     /gene="hisG"
                     /locus_tag="PLG72_06690"
     CDS             complement(394..1056)
                     /gene="hisG"
                     /locus_tag="PLG72_06690"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ80521.1"
                     /translation="MRYLSIALSKGRLADAAVEMLEKLGIDCSEYKAGSRKLIFTDES
                     NKIRFFLVKPADVPTYVEYGAADMGIVGKDTLLEEGRHLYEVLDLGFAACRMVVAGPA
                     ELAGKLDSLNNKRVATKYPRIAREYFEHKRRESIEVIKLNGSVELAPLVGLSEVIVDL
                     VESGRTLKENGLVVLDTIADISARLVVNRVSAKMESERINRIADGLRKQLEDKRSGDT
                     SR"
     gene            complement(1062..2318)
                     /gene="hisZ"
                     /locus_tag="PLG72_06695"
     CDS             complement(1062..2318)
                     /gene="hisZ"
                     /locus_tag="PLG72_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOJ80522.1"
                     /translation="MSKWKIYTPDGVQDILCDDCYQKRELEHKLRMLFRSCGFNEIEP
                     PAIEFYDVFASEGSGIMQENMFKFFDQQGRILVLRPDITVPVARITATKFKDAVWPLR
                     FSYIGNVYRYNDYGGGKQNEFTQAGVEILGVNTPESDAEVISIAIQALKTSGLENFQI
                     DIGQVDFFNGLMEETGLSEEEISQIRLLMNRKDNVGLGGFLKRTAIRDDLKQLISDLP
                     RFFGSSDVIEKARSYKLNEKSRAALDNISRILEILDDYGHSQYISVDLGMLRDLTYDT
                     GVIFRGFTYGVGFPILTGSRYDKLAGEFGKECPATGFTLGVNMVMMALQRQKIQAEKP
                     ATDTFVRYEREGRAAAFELCSVLRKQGLNVELDVSNMNVQEAARYAAEKNIGGVISIL
                     DEDNVEIHYIQSGETTRVKISELLKR"
     gene            complement(2583..2777)
                     /locus_tag="PLG72_06700"
     CDS             complement(2583..2777)
                     /locus_tag="PLG72_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80523.1"
                     /translation="MDIYDYSEESLAAIARSCRHLSYRHPAGSLNHPDSVSCIDCTHW
                     NGNGCSRNHLDSIASELHLD"
     gene            complement(3137..5650)
                     /locus_tag="PLG72_06705"
     CDS             complement(3137..5650)
                     /locus_tag="PLG72_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ80524.1"
                     /translation="MMTEANRQEFIRLRKELLEKEFGRLNPQQRQAVFQIKGPLLVLA
                     GAGSGKTSVLVNRIAYMIKYGNAYHSERVPANLTEEDIELMHKAAAGAAVTPEEQDRL
                     CTVMRDNPVHPSSILAITFTNKAAREMKDRLENMLGEGLEGIWVGTFHAACVRILRKH
                     IDKLGYSRNFVIFDTTDQQALAKDCIKELKLNESNFPVREVLSKISRAKDELREPQNY
                     AREAGSDYRLGKIAAIYELYQKKLKSNNALDFDDIIMQTIRLFVDNPDVMEEYQLKFR
                     YILVDEYQDTNYAQYMLVNLLSRNFRNLCVVGDDDQSIYSFRGADIRNILEFEKDFSD
                     TKVIKLEQNYRSTGTILDAANNVIKQNHGRKSKKLWTTNEKGEKIQYYEAEDEREEAS
                     FITSEIERLVKEEGRKYSDFALLYRVNAQSRALEEAFMRSGIPYRVFGGLKFYDRKEI
                     KDIIAYLRLIQNPSDDVALKRIINVPKRGIGNTTLGTAEELASERNCSIFAIVSSASE
                     IPELKRAADKLVAFADMIAGFKALSESLSVSELINEVINKSGILDELQAEDTEESRTR
                     VENIRELISGAIDFEAQSEEKGLEAYLANVSLVMDIDTMESEKDYVALMTLHSAKGLE
                     FPVVFIPGFEEGVFPGLRSMESDAALEEERRLCYVGITRAMEKLYFTSTHARTLFGST
                     SYNRCSRFMDDIPDDLIVMNRNRRSGEGGSTGRGSFGWDKGYGAGGRYNGEESFGPSG
                     VGGSVGTGRFSDTVRSGGTGSFKPIFGTETAGTSAGNGKAAAAAADYKPGDRVVHKKF
                     GVGTISNVTFDKGDQVLEIQFKNHGMKRLLAEFANLVRL"
     gene            complement(5960..6412)
                     /locus_tag="PLG72_06710"
     CDS             complement(5960..6412)
                     /locus_tag="PLG72_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80525.1"
                     /translation="MAVVFHPQAEMPQAAPVQPEPEKRPPQAAPAQQSPQPKPVQPSY
                     QPQGQKPPVAQTDMYAAPKKVEKTEPLSFWKFFGMLILQWIPIIGFIMILVWSFGSSF
                     NRNTKNYARAVLVLGIISFTLIVAGFIVNFAVIEQILDAFGYTIDFGW"
     gene            6443..6895
                     /locus_tag="PLG72_06715"
     CDS             6443..6895
                     /locus_tag="PLG72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ80526.1"
                     /translation="MIIHRIIKEYLNDSVYGDRVEELKKKTMEAADRSSLMERRAEEL
                     EREVEKLKKAEYMTYHIGEEFTGIISGVASFGFFVQIEGTIEGLVRVENLDEDYYIYE
                     AEAYRFIGRNSRKTYTLGDPVTVLVDSVDVTAREINFVLSDPDSRMTE"
     gene            complement(6864..8327)
                     /locus_tag="PLG72_06720"
     CDS             complement(6864..8327)
                     /locus_tag="PLG72_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOJ80527.1"
                     /translation="MSLNFPVIIKTLSFVTILVGVAMIPSALVSVFYAEYDVAVLFFM
                     IIVPVLAIGMALSVRIRATASRLRIREGYLAVALSWLLASLIGCLPYLATGTTDGFID
                     AFFEAVSGFTTTGASIVTNVEILPKGILFWRSFCQWLGGMGILVFAISILPALGIGGQ
                     NMAKAETPGISLSKMAPRMTDSAKILYIIYIVFSLAAGILFKRGGLGFFDSVIAALGS
                     MSSGGLSNYNEGISHFNSGYVEFVASFFTVLACINFTLYYNIIQGKWKQFFSNSELRT
                     FLIILAGGGLFIAANLWLTGSYDSIGESLRYGLFQSTVFITTTGHFSANYNLWPAFSK
                     AMLLLLMLIGASSASTGGGIKVIRIIALFKLIQKGIYMRLHPSAVVPVKIQGKIISSE
                     TVSGITSFLSLYAFVFVLSVLVLSLENLDFLTTFSTVVATLNNVGPGMEIVGPGGSFA
                     LFSGFSKLYLCFLMLMGRLELLTIALLFTPSFWNPDR"
BASE COUNT         1907 a         2303 c         1995 g         2265 t
ORIGIN      
        1 gggtcaatgg aatcgtaagc ctcctctatt tctcttcccg acacccttat ggacgaggcg
       61 tccaggtcca cttcatcata ctttttcgta tattcaatga ctgccctgtc cccgtttttc
      121 ctgacatttt caatgatctc ccgtactttg tccactacat cctgcatgcc gacgctttcc
      181 ctgtccgcaa gcttttcaaa aagttccgaa tctttcccct gtctcatatc gattattttc
      241 agcatgaaaa tctccccttc cttcagtttc gcgctgtcag cgccgaaccg ttttttgctc
      301 tgctgtaacg gctccgggat atcccggatc ttcccggcaa aagtatccct ccggcgggag
      361 tggccttttg ccgccggcct gttccatgtt ccgttacctg ctggtgtcgc cgctacgctt
      421 atcttcaagc tgcttcctca gcccgtcggc tatccggttt atcctttcgc tttccatttt
      481 tgcgcttacc ctgttcacga caagccttgc gcttatatct gctatggtat ccagcacgac
      541 caggccgttt tccttcagtg tccttccgct ttccaccagg tccactatca cttccgaaag
      601 tccgacaagc ggcgccagtt ccacggaacc gttgagtttt atgacttcga tggattcgcg
      661 acgtttatgc tcgaaatatt cccttgcgat acggggatat tttgtcgcga cccttttgtt
      721 gttgaggctg tccagctttc cggccaattc cgccggtcct gccacgacca tcctgcatgc
      781 ggcgaaaccc aggtccagca cctcgtacag gtgcctgccc tcctcgagca gcgtatcctt
      841 gcccacgatg cccatgtctg ccgccccata ctccacatat gtagggacat ccgcaggttt
      901 cacaaggaaa aatctgatct tgttggactc gtcggtgaaa atcagtttcc tggatccggc
      961 tttatactcc gaacagtcta tcccgagctt ttccagcatt tctacggcag catcggccag
     1021 tcttcccttg gacaatgcta tcgaaaggta tctcatactg ctcacctctt caacaactcg
     1081 ctgattttta ccctggtcgt ctcccccgac tgtatataat ggatctcgac attgtcctcg
     1141 tccaggatgc tgatgacgcc gcctatgttc ttttcggcag catacctggc agcctcctga
     1201 acgttcatgt tcgagacgtc cagttcgaca ttcagcccct gtttcctcaa aacggaacac
     1261 agttcgaatg ccgctgcccg gccttcccgc tcgtatcgca cgaatgtatc cgttgccggt
     1321 ttttctgcct ggatcttctg cctctgcaga gccatcatca ccatattcac tccaagagtg
     1381 aaaccggtgg cggggcactc cttcccgaac tcgcccgcaa gcttgtcata cctgctgcct
     1441 gtcagtatcg ggaatcccac tccgtaagtg aaccccctga atatgacgcc tgtatcgtac
     1501 gtcaggtccc tcagcatccc caggtccacg gatatgtact gcgaatgccc gtaatcgtca
     1561 agtatctcaa gtatccggct gatattgtcc agggcggctc ttgacttttc gttcagttta
     1621 tatgaccggg ccttctcgat cacatcggat gacccgaaga aacgcggcag atcggagatc
     1681 aactgtttca ggtcgtccct gatagccgtc cgcttaagga acccgccaag gcccacgttg
     1741 tctttcctgt tcatcagcag ccttatctgc gagatctctt cctcggaaag tcccgtctct
     1801 tccatcagcc cgttgaaaaa atccacctgc ccgatatcga tctggaaatt ttcaagtccg
     1861 ctcgttttga gtgcctgtat ggctatcgag atcacctcgg catccgattc cggcgtgttc
     1921 actcccagta tctcgacgcc tgcctgcgtg aactcgtttt gtttcccgcc tccgtaatca
     1981 ttgtagcggt atacgtttcc tatgtacgaa aacctcagcg gccatacggc atccttgaat
     2041 ttcgtcgccg ttatcctggc gacgggaacg gtgatatcag gcctcaggac cagtatcctt
     2101 ccctgctggt caaagaattt gaacatgttt tcctgcatta tcccgctgcc ttcggatgca
     2161 aatacgtcat agaattcgat tgcaggcggt tcgatctcgt tgaacccgca actcctgaaa
     2221 agcatcctca gcttatgttc cagttccctc ttctggtagc agtcatcaca aaggatatcc
     2281 tgcacgccgt cgggtgtgta tattttccac ttggacaaga agggcacccc ctgtaaccac
     2341 gatatatgct tccataacgc ctgccgtact ttcccggatg ccggccttcc gcgccttgcg
     2401 tccttccggg gacggcggtc cataatgcag gcgagaacat tcaagctttt tgctgcttca
     2461 ttgctttatc atgctaaatt gataaataaa tgaattgaga atattataaa atatggaaaa
     2521 ggatttgtca atgtttttta ttaaaaacat taaccggaca acagatgctc cggtcttcct
     2581 tgtcagtcga gatgcagttc ggatgcaatg ctgtcaagat ggttccttga gcatccgttt
     2641 ccgttccagt gggtgcagtc gatgcatgac acgctgtccg gatggttcag gcttccggca
     2701 ggatgcctgt agctcagatg cctgcaggac cgcgctattg cagcgagact ttcttcactg
     2761 tagtcataga tatccattcg ctacctccgg ataagataca gtaccggaaa ttccggtgca
     2821 tgatgtacac tgtattattt gcagatcatc cgcatgatat accgggcacg tgtaaatgcc
     2881 gggtatatga atatgccgga tatgcggata tgtcgtgtat atgaaaatgc cgtgtatgta
     2941 aaaacgccgg ctgtcagaat ccagccggca tttttgatcc gtcagaaaaa cctggttaca
     3001 tatccgatga aaaaccggat tcccgtataa aacataaaat cagatgcaga aaacccttaa
     3061 aaccgcacgg ccgcttcccc agaggcagcc ttgcggtcac agggggtgcc tcgcggttat
     3121 aaaggctgcc ttacagtcat agcctgacca ggttggcaaa ttcggccagc aaccgcttca
     3181 taccgtggtt tttgaactgt atctcgagca cctggtctcc cttgtcaaat gttacattgg
     3241 aaatggtacc gactccgaat ttcttgtgga ccacccggtc gccaggcttg taatctgctg
     3301 ctgccgcggc agccttccca tttccggcag atgtgcctgc cgtctccgtg ccgaagatcg
     3361 gtttgaaact gcctgtgccg ccggatctga cggtatcgct gaatctgcct gtgccgacgg
     3421 atcccccgac gccgcttggg ccgaagcttt cttcgccgtt atatctgccg ccggcgccgt
     3481 agcccttatc ccatccgaag ctgccgcggc ctgtggatcc gccttcgccg ctcctcctgt
     3541 tcctgttcat aacgatgaga tcatcaggta tgtcgtccat gaacctggag catctgttgt
     3601 atgaagtgct gccgaaaagc gtcctcgcat gggtactggt aaagtacagc ttttccatgg
     3661 cccgtgttat gccgacatag cagagtctcc tctcctcctc aagcgcagca tcgctttcca
     3721 ttgaacgcag gcccgggaat accccctcct cgaacccggg tatgaacacg acggggaact
     3781 cgagtccttt ggcgctgtgc agcgtcatca gggccacata gtccttttca ctttccatgg
     3841 tgtctatgtc cattaccagc gaaacattgg caaggtatgc ctcgagccct ttttcttcgc
     3901 tttgggcctc aaaatcgatg gcgccggata tcagttcccg gatgttttcg acacgggtcc
     3961 tcgattcctc ggtgtcctcg gcctgtagtt cgtcaagtat gcctgatttg tttatcactt
     4021 cattgatcag ttcggatacg ctgaggcttt cagacaatgc tttgaagcct gcgatcatgt
     4081 ctgcgaatgc cacgagcttg tcggctgcgc gcttcagttc aggtatctcc gatgcggacg
     4141 aaactatagc gaaaatgctg cagttccttt cggacgccag ttcctcggca gtgcccagcg
     4201 tcgtgtttcc tatccctctt ttgggtacgt ttattatcct tttgagcgca acatcgtctg
     4261 aagggttctg tatcagtctc aggtatgcga tgatgtcctt gatttccttc ctgtcgtaga
     4321 acttgagccc gccgaatacc cgatagggta tgcccgacct catgaacgct tcctcaaggg
     4381 cgcgcgactg ggcatttacc ctgtacagca gcgcaaaatc gctgtacttc cttccctctt
     4441 ccttcacaag cctctctatt tcactggtta tgaaggaagc ctcttccctc tcgtcctcgg
     4501 cctcgtaata ctgtatcttt tcgcccttct cattcgtggt ccagagtttc ttgctctttc
     4561 tgccgtggtt ctgctttatc acattgttgg ccgcgtcgag tatcgtcccg gtggaacggt
     4621 aattctgctc cagcttgatc actttcgtgt cgctgaaatc tttttcgaat tccaggatat
     4681 ttcgtatatc cgcgcccctg aagctgtata tggactggtc gtcatcgccc acgacgcaaa
     4741 ggttcctgaa gttccttgaa agcaggttta caagcatata ctgggcataa ttcgtgtcct
     4801 ggtactcatc gaccagtatg tacctgaact taagctgata ctcctccata acgtcagggt
     4861 tatccacgaa cagcctgatc gtctgcatta ttatgtcatc gaaatcaaga gcattgttgc
     4921 ttttcagctt tttctggtaa agttcatata tggcggctat tttgcccagc cggtaatcgg
     4981 atcccgcttc cctggcatag ttctgcggtt ccctcagttc atccttcgcc ctgcttatct
     5041 ttgaaagcac ctcccttacc gggaagttgc tttcgttgag tttcagttcc tttatgcagt
     5101 cctttgccag tgcctgctgg tctgttgtat cgaatatgac aaagtttctg ctgtatccaa
     5161 gcttgtcgat atgctttctc aggatcctga cgcacgccgc atggaaagtg cccacccata
     5221 tgccttccag gccttcgcca agcatgttct cgagcctgtc cttcatctct ctcgcagcct
     5281 tgttggtaaa agtgatggcc aatatggatg atgggtggac aggattgtcc ctcatgacgg
     5341 tacagagcct gtcctgttcc tcaggcgtga cggcggcgcc tgcggctgcc ttgtgcatga
     5401 gttcgatatc ctcttcggtt aaattggcag ggactctttc cgaatggtat gcgttgccgt
     5461 actttatcat gtatgcgatc ctgttcacaa gcaccgaggt cttccccgac ccggcgcccg
     5521 caaggaccag cagcgggccc tttatctgga aaacagcctg cctttgctgt ggattgagcc
     5581 ttccgaattc cttttcgagc agttccttcc ttaaccttat gaattcctgc ctgtttgctt
     5641 ctgtcatcat cagatcagca ccctgttttc attttatcgt atctcccatt atatccaacg
     5701 atctgccgcc gcgcaacaca aaaacaaaca tccgttcgag atgtcttttt attcctgcgt
     5761 ttgatttgct ggcgatcccg gaaatattgc tgtctggtat tccccggaat actgccgatc
     5821 ggtattccct gaaattccgc ccgttatccc ggatctgcgg cacaatatca aaaagagaca
     5881 ggcccggttc gcgggcgctg cctcttatgt atgccgtaaa tcctgtcata tgtcactgcc
     5941 gaattgcctc gtgccgtctt caccagccaa aatcgatggt gtacccgaac gcatcgagga
     6001 tctgttcgat gacggcaaag ttgacgatga agcctgctac gatcagtgta aaacttatga
     6061 tcccaagcac aagcactgct cttgcatagt ttttggtatt cctgttgaat gaacttccga
     6121 agctccatac gagtatcata atgaacccga tgatgggaat ccactgcaat atcagcatac
     6181 cgaaaaactt ccagaatgac agcggctcgg ttttttcaac cttcttagga gctgcataca
     6241 tgtccgtctg agcaacaggc ggtttctgcc cctggggctg gtatgacggc tgtaccggct
     6301 ttggctgggg tgactgctgt gccggtgctg cctggggagg tcgcttttca ggttccggtt
     6361 gcaccggtgc tgcctggggc atttcggcct ggggatgaaa cactactgcc atttcacagc
     6421 tcccatccgc agatacccgg atttgatcat tcacaggatc atcaaggaat atttaaacga
     6481 cagcgtatat ggagacaggg tggaggagct caaaaagaaa accatggaag cggcggatcg
     6541 ttcctctctg atggaacgca gggctgaaga gttggaacgg gaagttgaaa agctgaagaa
     6601 agcggaatat atgacctatc acatcggtga ggagttcacc ggtattataa gcggagtggc
     6661 ttccttcggg ttcttcgttc agattgaggg aaccatagaa gggcttgtcc gggtggagaa
     6721 cctggatgag gattattata tatatgaagc ggaagcgtat cgcttcatcg ggcgcaacag
     6781 caggaaaacc tatactctgg gagatccggt taccgtattg gtggattcgg tggatgtgac
     6841 ggcgagggaa atcaattttg tcctcagcga tccggattcc agaatgacgg agtgaagagc
     6901 aaagcaatag tgaggagttc cagcctgccc atgagcatga gaaaacaaag gtagagcttt
     6961 gagaaaccgg aaaaaagggc aaaggatccg cccgggccca cgatctccat gcccggtccc
     7021 acgttgttca gcgtggctac taccgtggag aaggtggtga gaaagtccag attctcaagg
     7081 gaaagaacca agacgctcag cacaaaaaca aatgcgtaca gggaaaggaa agacgtaatc
     7141 cccgataccg tttcggaaga tatgatcttt ccttgtattt ttacgggcac aactgcactg
     7201 ggatgtaatc tcatgtaaat gcctttttgg ataagcttga acagcgcgat tatccgtatc
     7261 accttgatcc cgcctcctgt ggatgctgaa gaagctccga tgagcatcag cagaagaagc
     7321 attgctttgc tgaaagccgg ccaaaggtta taattcgcgc tgaaatggcc ggtggtggta
     7381 atgaagacgg tggattggaa aaggccgtat cgcagtgatt ctcctataga atcgtagctg
     7441 cctgtgagcc aaaggtttgc cgcaatgaaa agccctcccc ctgcgaggat aatcagaaaa
     7501 gtccgaagct cactgttgga aaagaattgc ttccatttcc cctgaataat gttgtagtaa
     7561 agagtgaaat tgatacaggc gaggacggta aagaaagacg caacaaattc aacgtatccg
     7621 ctgttgaaat gagagattcc ttcattgtaa ttggataagc cccccgaaga catactaccc
     7681 agagctgcaa taacggaatc aaaaaagccc aaacctccac gcttaaaaag gatgccagcc
     7741 gccagggaaa aaacgatgta gattatatag agtatttttg ccgaatcggt catccttgga
     7801 gccattttgc tcagggagat gccgggggtt tccgccttgg ccatattctg gccgcctatt
     7861 cccagagccg ggagaatact gatagcgaaa accaggatac ccatgccgcc caaccattgg
     7921 cagaaggagc gccaaaacaa aatccccttc ggcaggattt ccacgtttgt tacgatggaa
     7981 gcccctgtag ttgtaaatcc ggaaacggct tcaaaaaagg cgtcgataaa tccgtcggtg
     8041 gttcccgttg ccaggtatgg aaggcagccg atgagggaag ccaaaagcca gcttaaggct
     8101 acggcaaggt aaccttcccg aatcctaagc cttgatgccg ttgctcttat cctgacactt
     8161 aaggccatac cgatggccag aaccggaact atgatcatga aaaacagtac tgccacatcg
     8221 tactccgcat aaaatacgga caccagggcc gaggggatca tggccacccc gacgaggatg
     8281 gtgacaaagc tgagagtttt aataatcaca gggaaattca gcgacataaa cagctccttg
     8341 taaatcagta atccaataga gttatctttt gtcaggagat aaagccccat ttcctgggct
     8401 gaaaaaactt ctccaacttc ggcagctggg aaagtaagaa caagatgatg accttatccc
     8461 cttcttctat
//
LOCUS       DAOSCC010000102         8465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5465_length_8465_cov_4.070868, whole genome shotgun sequence.
ACCESSION   DAOSCC010000102 DAOSCC010000000
VERSION     DAOSCC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5465_length_8465_cov_4.070868"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="PLG72_06725"
     CDS             <1..1900
                     /locus_tag="PLG72_06725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOJ80528.1"
                     /translation="EVAPIPPGTYPPNIEGAEQEIQRLTYARAHEIYGDPLPEIVSKR
                     LEKELNSIIRNGFAVMYLIAQKLVKKSNDDGYIVGSRGSVGSSLVATMSGITEVNPLP
                     PHYICRKCKHSEFITDGSVGSGFDLPAKKCPECGELMRGDGHDIPFETFLGFDGDKAP
                     DIDLNFSGEYQARAHKYTEVIFGKDHVYRAGTIASVADKTAYGFVMKYLEEKGITATN
                     AEINRLVAGCTGIKRTTGQHPGGIIVIPSDKDVHDFTPIQRPADDTSSDVITTHFDFH
                     SLHDTILKLDILGHDDPTMIKMLCDLTGMNIKDVPVNDEKVMQLFVSTEPLGIRPEDI
                     NCEVGTFALPEFGTRFVRQMLLDTKPKTFSDLLQISGLSHGTDVWLNNAQDLIRDGVC
                     TISEVIGTRDSIMIYLMYQGLPPKSAFKIMEDVRKGKGLKPEYEEEMKAHGVPGWYIE
                     SCKKIKYMFPKAHAAAYVLMAMRIGWFKVYHPLAFYTAYFTVRADEFDATTMASGKKN
                     VEKRIEELEKQGNSISAKDRNVLTILEVANEMYARGIEFLPVDLYESDAVRFRIQGNG
                     IRPPLNSLPGLGANAAKAIADARGQGEFISIDDLKLRAKVSKSVIEILQQSGCLDGIP
                     ESSQISMF"
     gene            2134..2595
                     /gene="rimP"
                     /locus_tag="PLG72_06730"
     CDS             2134..2595
                     /gene="rimP"
                     /locus_tag="PLG72_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOJ80529.1"
                     /translation="MAKKKVEDIVEELAKPVVEALSFELVDVEYLKEGANWYLRIYID
                     KPGGVTIDDCQAVSEQMSSILDREDPIKQSYMLEVSSPGERPLKKDRDFERFRGEFVE
                     VKLYQPLNGKKIYEGELLGLNGDIIEIRTDDGVTMGFDRKDTALVRRIIRF"
     gene            2673..3926
                     /gene="nusA"
                     /locus_tag="PLG72_06735"
     CDS             2673..3926
                     /gene="nusA"
                     /locus_tag="PLG72_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOJ80530.1"
                     /translation="MSAELIRALDQLEKERGIEKEVLIEAIEAALVSAYKKNFGTSQD
                     VRISIDRDTGEVKVFALKKVTARPMDENSEISLEDALKLDSRYAEDDIAEVEVTPRKF
                     GRIAAQTAKNVVLQRIREAERNLIYEEYFNKEGDIVTGIVQRIERRNVIIDLGKAEAI
                     MLPPEQTNGEEYRFNDRIKTYIVEVRKTTKGPQILVSRTHPGLVKRLFELEVPEIHDG
                     TVEIKSISREAGSRTKIAVYSKDPNVDPIGACVGQRGTRVQAIVDELRGEKIDIIKWS
                     SDPEEYISSSLSPAKVVRVDVDEETRTARVIVPDFQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSESQFRAAIEQQIFSMDSGYDGETADEDEEDYDEEYDGTNDGTEEDGFEDHEDG
                     TETADGGGTDPDESDPEQDSEPEQE"
     gene            3932..4216
                     /locus_tag="PLG72_06740"
     CDS             3932..4216
                     /locus_tag="PLG72_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ80531.1"
                     /translation="MKKKRIPLRMCTGCHEMKPKKELIRIVRDKEGAISIDLTGKKSG
                     RGAYICRNVECLRSARKGKKLERAFEAPVDDAVYAALEEQLVEQTERNDG"
     gene            4206..4535
                     /locus_tag="PLG72_06745"
     CDS             4206..4535
                     /locus_tag="PLG72_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOJ80532.1"
                     /translation="MMDNRVYSFLGLATKAGKLISGDETCERAIKAGKARLVIVTEDA
                     SGNTRKKFMDMCSYRNIDIRCFGKKEKLGRHIGKSVRSVVAVIDGSFAERLLEMIDDH
                     GKEYGGV"
     gene            4537..7683
                     /gene="infB"
                     /locus_tag="PLG72_06750"
     CDS             4537..7683
                     /gene="infB"
                     /locus_tag="PLG72_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ80533.1"
                     /translation="MEKVRVYELAKELNTTSKRLMEKLAEINIIVKNHMSLLDEKELE
                     ALYRHIGVIRHDDKKSEAEEPKTAPAVPVQPKPELRRDKKNAPCIIRTTEIVINTKDD
                     SHTFGSGYGKDSHKDSQKNIRNSYIRSDDSNSGLLSGFVRKSRTDYIETMIAARKQET
                     TRKEQEPAASAGQTEKNGKVVAKPETKTDTQPEKKEKAADRVQAQKPDIQKADVQKPD
                     TGKDVLKERKTQAEAADAAREKKAKPAEAKGREADTDARTADKPKQTVTGAAAEVKPV
                     QPEKAAQTGKPADSAAVEAQKAGKSAAHATEKFRGEGQQRKDTRPLTAKPQHQAPKPQ
                     YDRGQYQGARTGGGYKPAGKQLDIPMPDFAAVQKDETASQRGESRREFSGKDFDKDAK
                     REQRKEALKVVPSKPSKKIKPTNIGLHEKKGVSEILSEDFLFDDFYDEAGIRKKKLSR
                     QKKSKEENKHIPPKAVLTTIKIPESVTVKELAEALKKTSSEVIKKLMGYGIMATLNQE
                     VDFDTAAVVAEEFGVRAEKEVILSEEDILFDDEPDDPEKLVPRPPVVVVMGHVDHGKT
                     SLLDAIRSTHVIDSEAGGITQHIGAYTVQINGRNITFLDTPGHEAFTAMRARGAQVTD
                     VAILVVAADDGVMPQTIEAINHAKSANVSIIVAINKIDKPGANPDKVKQDLTQYGLVP
                     EEWGGDVICVEISAKKRINIDQLLEMVLLTADILELKADPTRQAKGTVIEAKLDKNRG
                     PVATLLVQRGTLNVGDSIITGTTVGRIRAMMDDKGKSIKSAGPSTPVEILGLPEVPVA
                     GETFYAVTDEKLARQLAEKRRIRQQQEHQHTAAKVSLDGLFKQIQEGKVKDLNLIIKA
                     DVQGTVEALKQSLEKLSNDEVRVNVIHGAVGGITESDVTFADVSNAIIIGFNVRPMPN
                     VSEAAKRAGVDIRLYNVIYNAIEDMEAAMKGMLEPTYREVVIGHVEVRQIFRISGIGT
                     IAGCYVLDGRITRNSDVRVVRDGIVVHEGHLASLKRFKDDVREVQQGYECGLSIEKFN
                     DIKENDIIEVFVMEEVKR"
     gene            7793..8179
                     /gene="rbfA"
                     /locus_tag="PLG72_06755"
     CDS             7793..8179
                     /gene="rbfA"
                     /locus_tag="PLG72_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ80534.1"
                     /translation="MDRTDRISEEIKREISFIIQNELKDPRLGKMISVTAVDVTRDLR
                     YAKVYVSVLGDENDKKDAASGLKSASGFIRREIGRRVQLRYTPEIIFELDDSIERGVY
                     INKLINDTIQDNRRGTHAEEDSGRDG"
     gene            8148..>8465
                     /locus_tag="PLG72_06760"
     CDS             8148..>8465
                     /locus_tag="PLG72_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ80535.1"
                     /translation="MLKKIAEEMAKAGSIVILPHIAADGDAIGSSVALAIGLSGAGKE
                     VSVLLEEKAPQMYNFLPGLEMARVYDADDRKYDLAVALDCGDPERLGSRRPVFEKAPV
                     TVNI"
BASE COUNT         2578 a         1866 c         2460 g         1561 t
ORIGIN      
        1 ggaggtggct ccgatacctc ccgggacata tccgccaaat atagagggcg ccgaacagga
       61 gatacaaagg cttacatatg caagggcaca tgagatctac ggtgatcctc ttcctgaaat
      121 agtgtcaaaa aggcttgaaa aagaactcaa ctcgatcatc aggaatgggt ttgccgtcat
      181 gtatctgata gcgcagaaac tggtgaaaaa atccaacgat gacggatata tcgtaggttc
      241 gaggggatcg gtgggttctt cgctggtcgc gacgatgtcg gggataacgg aggtgaatcc
      301 cctgccgccc cactatatct gccggaaatg caaacattcg gagtttataa cggacggttc
      361 cgtcggatcg gggttcgacc tgcctgccaa aaaatgtcct gaatgcggcg aactgatgag
      421 aggcgacgga catgacatac cgttcgagac cttcctcggt ttcgacgggg acaaggcccc
      481 tgatatcgat ctgaacttct ctggcgagta ccaggccaga gcacataaat acacggaggt
      541 cattttcgga aaagaccatg tctacagagc cggtaccata gcttccgtcg cggacaagac
      601 ggcttatggc ttcgtcatga aatacctgga ggagaaaggt atcactgcta cgaacgctga
      661 aataaacagg cttgtggcag gctgcaccgg tataaagagg accacgggac agcatcccgg
      721 aggcataatc gtcataccgt cggacaagga tgtgcatgat ttcacaccca tccagaggcc
      781 ggctgacgat acgtcctcgg atgtgataac cacgcatttc gacttccatt cactgcacga
      841 cactatactc aagctggaca tcctggggca tgatgacccg accatgatca agatgctctg
      901 cgacctgacc ggcatgaata taaaggatgt gccggtcaac gatgaaaaag tgatgcagct
      961 gttcgtctca acagagcccc tcgggatcag gcctgaggat ataaactgcg aggtgggcac
     1021 atttgccctg cctgaattcg ggacaaggtt cgtaaggcag atgctgctgg ataccaaacc
     1081 caagacgttt tccgatctgc tgcagataag cggactttcc cacggcactg acgtttggct
     1141 gaacaatgcc caggatctta tcagggacgg tgtctgtaca atatccgaag tcataggcac
     1201 cagggacagt ataatgatct acctgatgta ccaggggctt ccgccaaaat ccgcattcaa
     1261 gatcatggaa gatgtcagga agggaaaagg ccttaaaccg gaatatgaag aagaaatgaa
     1321 agcccatggc gttcccggat ggtacatcga gtcatgcaaa aagataaagt acatgttccc
     1381 caaagcccat gctgcagcct acgtgctgat ggcaatgagg ataggatggt tcaaggtata
     1441 tcatccgctt gccttctata ctgcgtactt tacagtcagg gcagatgagt ttgatgccac
     1501 cacgatggca tccgggaaaa agaatgtgga aaaaaggata gaggaactcg aaaaacaggg
     1561 caattccatt tcggcgaagg acaggaacgt gcttacgata cttgaagtcg ccaatgaaat
     1621 gtatgcgaga ggcatagagt ttcttccggt ggatctctac gaatctgatg cggtcaggtt
     1681 caggatccag ggcaacggca tcaggccgcc gctgaactcg ctgcccgggc tcggggccaa
     1741 cgctgcgaag gccatagcgg atgcgcgcgg gcagggtgag ttcatttcca tcgacgacct
     1801 gaagcttagg gcaaaggtca gcaagagcgt catcgagata cttcagcaaa gcggatgcct
     1861 cgacggcatt cccgaaagca gccagataag catgttctga caaacaggta cggacatgca
     1921 tgattcgttt gcggggactt ttccggggtt cctccgagga tcctgaaaac ccgcagaaca
     1981 gtaaaaaact gaaataaaag gattgataaa tgtaaacaat aatgatataa tacaccgtgg
     2041 accaagtata tcttttttta ggcaaagagt gggcttaccc cactctttgt ctttagcgac
     2101 aggctgcatg gccggaagga acggagatga acgatggcga agaagaaagt ggaagatatc
     2161 gtggaagaac ttgcaaagcc ggtagttgaa gcattgtcct tcgagctggt ggacgtggaa
     2221 tacctgaaag aaggcgccaa ctggtatctg aggatataca tagacaaacc cggcggagtt
     2281 accatcgatg actgccaggc tgtgagcgag cagatgagca gcatactgga cagggaagat
     2341 ccaataaagc agagctacat gctggaggta tcatctccag gcgaaaggcc gctgaagaag
     2401 gacagagact ttgaacgatt caggggcgag ttcgtagagg tgaagcttta ccagccgctc
     2461 aacggcaaaa agatatatga gggtgagctg ctcggcttga acggagacat tatagagata
     2521 aggacagatg acggtgtcac aatgggcttt gataggaagg atacagccct tgtcagacgg
     2581 atcatccgtt tttagctggc attccatccc gcaggcgggg ccatgcttgt atgtaaattc
     2641 ccgtatacag aggtattaca ggaggtaaaa atatgagtgc ggaattgatt cgtgcattgg
     2701 atcaactgga aaaagaaaga ggaatagaga aagaagtttt gattgaggca atcgaagctg
     2761 ctctcgtttc agcgtacaaa aagaattttg gtacctcaca ggacgtcagg atatctatag
     2821 accgtgacac cggcgaagtg aaagtgtttg cgctcaaaaa agtcacagcc aggcccatgg
     2881 atgaaaacag cgagatttcg ctggaagatg cattgaagct ggacagcaga tatgctgagg
     2941 atgatatagc agaggtagag gtcacgccga gaaaattcgg aaggatagcc gcacagacgg
     3001 cgaagaacgt tgtgctgcaa aggatacggg aagcagaacg caacctgata tacgaagaat
     3061 atttcaacaa ggaaggcgac atcgtcaccg gcattgtcca gcgtatcgag cgcaggaatg
     3121 tgataataga tctcgggaaa gccgaagcca tcatgcttcc gcctgagcag accaacggag
     3181 aagaatacag gttcaatgac aggataaaaa catatatcgt ggaagtcagg aaaaccacaa
     3241 aaggtccgca gatacttgtt tccaggacac atccgggcct tgtcaagagg ctttttgaac
     3301 tggaggtccc tgagatacac gacggaacgg tcgaaataaa gagcatatcc cgcgaagccg
     3361 gatccaggac gaagatcgcc gtttattcaa aggatcctaa cgtcgacccc atcggcgcct
     3421 gtgtagggca gagggggacg agggtccagg ccatcgttga tgaactcagg ggtgagaaga
     3481 tcgacataat caaatggagc agcgatcccg aggaatatat ctccagcagc ctcagccccg
     3541 caaaagtcgt gcgggtcgac gtcgatgaag aaaccaggac ggcaagggtc atagtaccgg
     3601 atttccagct ttcgctggcg atcgggaaag aaggccagaa tgcaaggctt gcagccaaac
     3661 tgaccggctg gaagatagat ataaagagcg agtcgcagtt cagggccgcc atcgaacagc
     3721 agatattcag catggacagc ggatatgacg gcgaaacagc agatgaagac gaagaagact
     3781 atgatgaaga gtatgacgga accaacgacg gcactgaaga agacggtttc gaggatcatg
     3841 aagacggaac cgaaaccgcg gacggcggcg ggaccgatcc ggatgaatca gatccggaac
     3901 aggacagtga accggaacag gagtgaggcg cttgaagaaa aaaaggattc cattgcgcat
     3961 gtgcacagga tgtcatgaga tgaagcccaa aaaggagcta atcaggatcg tcagggacaa
     4021 ggaaggcgcg ataagcatag acctgacggg caagaagtcc ggcagaggcg catacatctg
     4081 caggaacgtt gaatgcctca gaagcgccag gaaagggaag aagctggaaa gagcatttga
     4141 agcgccggtg gatgatgctg tatatgcggc actggaagag caacttgtgg agcagactga
     4201 gagaaatgat ggataacagg gtatactcat tcttggggct ggcgacaaaa gccggaaagc
     4261 tgatttcagg cgatgagacc tgcgagagag ccataaaggc cgggaaagcc cgtcttgtta
     4321 ttgtaacgga ggacgcttcc ggcaatacaa ggaaaaaatt catggatatg tgcagttaca
     4381 gaaacattga cataagatgt tttggaaaga aggagaagct tgggagacat atcggcaaaa
     4441 gtgtgcgttc agtcgttgcc gtgattgacg ggagctttgc agagcgtctt ctggaaatga
     4501 tcgatgatca cggtaaagaa tatgggggtg tataatttgg aaaaagtcag agtatatgaa
     4561 ctggcaaagg aactgaatac gacaagcaag aggctgatgg agaaactagc ggagatcaat
     4621 atcattgtaa agaaccatat gagccttctt gacgaaaagg agcttgaggc cctttacagg
     4681 catatcggcg tgatcaggca tgatgataaa aagagcgagg cagaggaacc caaaactgcg
     4741 cccgccgtgc ctgtgcagcc caagcctgaa ctgaggagag acaaaaagaa tgccccctgt
     4801 attataagaa cgacagagat agtaataaat acaaaagacg acagtcatac attcgggagc
     4861 ggttacggca aagacagcca caaagacagc caaaagaata tcaggaacag ctatatcaga
     4921 tcagatgaca gcaattcggg cctgctttcc ggttttgtga gaaagagcag gaccgactat
     4981 atcgaaacga tgatcgctgc caggaaacag gaaacgacca ggaaggagca ggaacctgca
     5041 gcctcagccg gccagacaga gaagaacggc aaagttgtcg cgaaaccgga gacaaagaca
     5101 gatacgcagc cggagaaaaa ggaaaaggca gcagaccgtg tccaggctca gaaaccggat
     5161 attcagaagg ctgatgttca aaagcccgat acggggaagg atgttctgaa agaacggaaa
     5221 acacaggcag aagcagccga tgcggccagg gagaaaaaag cgaagccggc tgaagcgaaa
     5281 ggcagggaag cagatacaga cgccaggacg gctgataagc cgaagcagac cgtcacaggc
     5341 gccgctgccg aagtgaagcc tgtacagccg gaaaaggcgg ctcagaccgg caaacccgcc
     5401 gacagtgcgg cggttgaagc tcaaaaagcc ggaaaaagcg ctgcccatgc aactgaaaag
     5461 ttcaggggag aaggccagca gcggaaggat accaggcccc tgactgcaaa accacagcat
     5521 caggctccaa agccccagta tgacaggggg cagtaccagg gagcgagaac aggcggagga
     5581 tacaagccgg ccggcaaaca gctcgacatt ccgatgcctg atttcgcagc cgtacagaaa
     5641 gatgagactg cctcgcagag aggggagagc aggcgtgaat tctcgggcaa ggattttgac
     5701 aaagatgcaa aaagagaaca gaggaaagag gctctgaagg tcgttccgag caaacccagc
     5761 aagaagatca aacctaccaa tataggtttg catgaaaaga aaggggtttc agagatcctt
     5821 tccgaggatt tcctttttga tgatttttat gatgaagccg gcatcaggaa aaagaagctg
     5881 agcaggcaga aaaagagcaa ggaagagaat aagcatattc cgccgaaagc agtgctcacc
     5941 acgataaaaa tacctgagag cgtaacggtg aaagaactgg ccgaagcttt gaaaaaaacc
     6001 tcttccgagg tcatcaaaaa gctgatggga tatggaataa tggcaactct caaccaggaa
     6061 gtggatttcg acaccgctgc ggtcgtagcg gaagagtttg gcgtaagggc agaaaaggaa
     6121 gtcatcctca gcgaagaaga catcctgttc gacgatgagc ctgacgatcc ggaaaagctt
     6181 gtacccaggc cgccggtggt agttgtcatg ggccatgtcg accatggaaa aacttcgctg
     6241 cttgacgcga tcaggtcgac ccatgtgata gacagtgaag caggcggaat cacgcagcat
     6301 ataggcgcat ataccgtcca gatcaacggc aggaacatca ctttcctcga taccccgggc
     6361 catgaagcct ttaccgcgat gagggcaagg ggcgcccagg tgaccgacgt tgccatactc
     6421 gttgtggcag cggatgacgg tgtcatgccg cagacgatag aggcaataaa tcatgcgaaa
     6481 tcggcaaatg tatcgataat cgtcgcaatt aacaagatag acaaaccggg ggcgaacccg
     6541 gataaagtca agcaggatct tacacagtac gggctcgtgc ctgaagaatg gggcggagac
     6601 gtgatctgtg tcgagatatc ggcaaagaag cgcataaata tcgaccaact gctcgaaatg
     6661 gtgctcctga cggccgatat actcgaactt aaagccgacc cgacaaggca ggcaaaaggc
     6721 accgtcattg aagcaaagct tgacaagaac agaggcccgg tggctacact gctggtgcag
     6781 agaggtacgc tgaatgtcgg tgattcgatc atcaccggta caactgtcgg caggatcagg
     6841 gccatgatgg acgacaaggg caaatcgata aagagcgccg gtccgtcaac accggtggaa
     6901 atactcggac tgcctgaagt gccggttgca ggcgagacgt tctatgcggt cacagatgaa
     6961 aaactcgcaa ggcagttggc tgaaaagcgc aggataaggc agcagcagga gcaccagcac
     7021 actgccgcca aggtgtcgct tgacggcctg ttcaagcaga tacaggaagg caaggtcaag
     7081 gacctcaacc tgatcatcaa ggcggatgta caggggacgg tggaggccct gaagcagtcc
     7141 ctcgagaaac tgtccaacga cgaagtgagg gtcaacgtga tccatggggc cgtcggcggt
     7201 atcaccgaat ccgacgtaac gttcgcagac gtctccaatg ccataatcat cggcttcaat
     7261 gtcaggccga tgcccaatgt ttcagaggca gccaagaggg caggcgtaga catccgtctg
     7321 tacaatgtca tctacaatgc catagaggac atggaggcgg ccatgaaggg catgctcgaa
     7381 ccgacataca gagaggttgt gatcgggcat gtcgaagtaa gacagatatt caggatatca
     7441 ggcatcggga ccattgcagg ctgctatgtc ctggacggca ggataacaag gaacagtgac
     7501 gtcagggtcg tcagggacgg tatagtggtc cacgaaggcc acctcgcatc gctcaagcgt
     7561 ttcaaggacg acgtaagaga ggtgcagcag ggttatgaat gcgggctttc gatagagaaa
     7621 ttcaatgata tcaaggagaa tgatattatc gaagtgttcg ttatggaaga ggtaaaacgc
     7681 tgacggcgtt atcagcatgc ggcaaggctg gacaggcggg tcatactgtc tgcccggcct
     7741 ttttgtaaaa gtggctgtcc gccgggcagc atgtgccgaa aggagtggtt ttatggacag
     7801 gacagacagg atatccgaag aaataaaaag agagatcagc ttcataatcc agaatgaact
     7861 gaaggatccc aggctcggca aaatgatcag cgtgacggcg gtggatgtca caagggatct
     7921 gagatatgca aaggtctatg tcagcgtgct tggggacgaa aatgacaaaa aggatgcggc
     7981 cagcggcctg aaaagcgctt caggattcat aaggcgcgag ataggacggc gggtacaatt
     8041 gcggtatacg cctgaaatca tatttgaact ggatgattcc atagaacgcg gggtatatat
     8101 aaataaactt ataaatgata cgatccagga caacaggaga gggacccatg ctgaagaaga
     8161 tagcggaaga gatggctaaa gccggcagca tagtgatact gccccatata gcggctgacg
     8221 gggatgccat tggctcatcc gtcgcgcttg cgatcggatt gtccggtgca ggaaaggaag
     8281 tttcggtact tctcgaagag aaggcacccc agatgtataa tttccttccc ggacttgaga
     8341 tggcccgcgt atatgatgcc gatgacagga agtatgatct cgcggtcgca ctggactgcg
     8401 gcgaccctga aaggctcggc agcagaaggc ccgtatttga gaaggcacct gtaacggtaa
     8461 atatc
//
LOCUS       DAOSCC010000103         8281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5618_length_8281_cov_4.987843, whole genome shotgun sequence.
ACCESSION   DAOSCC010000103 DAOSCC010000000
VERSION     DAOSCC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_8281_cov_4.987843"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLG72_06765"
     CDS             complement(<1..298)
                     /locus_tag="PLG72_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOJ80536.1"
                     /translation="MIHKFRMHGTNIILDVNSGTVHVVDDTAYEMTDELDKPDKAIIE
                     RFSAKYGRDEARETLAELRELQRAGQLFSEDIYEEYLAQNPVSGDGEPTVKALCL"
     gene            complement(459..599)
                     /gene="scfA"
                     /locus_tag="PLG72_06770"
     CDS             complement(459..599)
                     /gene="scfA"
                     /locus_tag="PLG72_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOJ80537.1"
                     /translation="MKHIKTINAPVSKKDISSSGCGECQTSCQSACKTSCTVANQKCE
                     KR"
     gene            complement(684..1121)
                     /locus_tag="PLG72_06775"
     CDS             complement(684..1121)
                     /locus_tag="PLG72_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOJ80538.1"
                     /translation="MPARKSTGIKQEEYEGLNPLLLLKGLLASYIITIPAFLLFALIL
                     ANVDFPQRLVTPVVVVITVISVITAGAVSTRGVRSKGWLNGSIVGLIYMIILYLFSSI
                     VFNNFAVDRNVLTMTIIGVLSGAIGGIMSINTKKSPRYKYIDG"
     gene            complement(1329..1619)
                     /gene="yajC"
                     /locus_tag="PLG72_06780"
     CDS             complement(1329..1619)
                     /gene="yajC"
                     /locus_tag="PLG72_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOJ80539.1"
                     /translation="METEGISMWLRTLGCLVFFILMMYLLIFLPQKRRDKKAKEMLDS
                     MQVGHQVITIGGISGKVVNIKDDEVTIETGVEKAKINLKKWAIKEVIKPIES"
     gene            complement(1684..2805)
                     /gene="tgt"
                     /locus_tag="PLG72_06785"
     CDS             complement(1684..2805)
                     /gene="tgt"
                     /locus_tag="PLG72_06785"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ80540.1"
                     /translation="MSAIRYELIKKCKQTGARLGIVHTPHGSFETPAFMPVGTQATVK
                     CMSPDELKEIRAGIILSNTYHLYMRPGSELIREAGGLHRFMNWDRPILTDSGGFQVFS
                     LSDLRNITEEGVTFRSHIDGSKHMFTPELAIKIQNDLGSDIIMAFDECIPYPADHDYA
                     KKSMERTTRWAERCREAHKDTEKQALFGIVQGGVYEDLREKSARDLVKLDFPGYSIGG
                     LSVGEPPQDMYRILEYTVPLLPEDRPRYLMGVGSPDYLVEGAIRGIDMFDCVLPTRIG
                     RNGTVMTSEGRIIVRDAKYARDFSPLDPKCDCYACKNFTRAYIRHLLKAQEILGLRLT
                     TLHNLKFLLDLMANVRQAIMEDRLGDFREEFFSSYGYKL"
     gene            complement(2838..3863)
                     /gene="queA"
                     /locus_tag="PLG72_06790"
     CDS             complement(2838..3863)
                     /gene="queA"
                     /locus_tag="PLG72_06790"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ80541.1"
                     /translation="MKTSDFYYELPQELIAQEPLEDRSSSRLMVLDRKSGKIEHRIFR
                     DIKEYLKPGDCLVLNDTRVMPARLLGAREDTGGSIEFVLLKNISGDIWEVILKPGRRA
                     KPGARFVFGNGLLKAEILDVVEGGNRIVQFEYEGIFQQVLDKVGIVPLPPYITKKLQD
                     PERYQTVYSRYMGSAAAPTAGLHFTKELLDDLGRSGIYTAYLTLHVGLGTFRPVKTEN
                     VEDHVMHSEVYNLSSETADVINRARTEGGRIIAVGTTSCRVLETVSSDDGLVKPSSGT
                     TDIFIYPGYRFKAIDGLITNFHLPESTLLMLVSAFAGRENILDAYRIAVEQRYRFFSF
                     GDAMFII"
     gene            complement(4052..5986)
                     /locus_tag="PLG72_06795"
     CDS             complement(4052..5986)
                     /locus_tag="PLG72_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOJ80542.1"
                     /translation="MAKSIIRKITALLAASAVFAVIFSANAGTGLASNTNEETIRVGL
                     YYKGSSVDTAQSIFDVSAQAGVRAGFVNSGGTFTEVCSFTGSSAVYVRKDAYYHTSGT
                     SLKEFSGSGSNITGKKYGPYHVKIGTDYPDAASAMNSVHSYRQAGIDAYIAYNDAWQV
                     WTGFCMDTAEAEAMIRELKSLFWETGFEIVTPSSNRVVVTDGQYQTLCVFGSKQYFLQ
                     LRPATGADSAVINIKGKPYRGCVEVKRLSNSDMTVINVVSLREYLYGNVPPEIGGKSP
                     SEALKAQAVVSKMYALNNRGKHGSSGFDICATTHCQVYKGYSVEVAECRKAIDEVYDK
                     IITYNGKPIEHVYYFASGGGSTEDVRNVWGSSYPYLVSVKDDYEKIYTWSKTLRASDI
                     KSMLPELGSILGVTITRTAPTGRVTQLAVTGSSRGEPLYFSNERCRTLFGLDSQLYTI
                     TTDADVYLAGLSEIPVMQSADSTAGKDGAQDVNVIAKEEIPASGPDMESGTGTQDPRG
                     GTDGQAGTNGGNQDKDETEISTPEAGKNGETAEKVTSLPVVAAPVASEPIKTQLGGKK
                     VVTAGGITTISGSNNKITILGADGAVNKAAVIPETYTFTGKGWGHAVGMSQEGAIGMA
                     KAGKTYEEILTYYFQGTKVE"
     regulatory      6241..6416
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6507..7583
                     /gene="asd"
                     /locus_tag="PLG72_06800"
     CDS             6507..7583
                     /gene="asd"
                     /locus_tag="PLG72_06800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ80543.1"
                     /translation="MNSKLRAGVIGGTGMVGQRFITLLENHPWFQVTAVAASERSAGL
                     PFEKAVEGRWKLDVPIPESVKNITVINAADIDRMADEVDFVFCAVDMPKDAVRELEEK
                     YARAEIPVISNNSAHRSTEDVPMIIPEVNPQHTAIIEKQRKRLGTRKGFIAVKSNCSL
                     QSYVPPVHALMQFGPEKILASTYQAISGAGKVFREWPEIIDNVIPYIGGEEEKSEKEP
                     LKIWGTIEGDVIKPASGPAVSAQCYRVPVSDGHLAAVYVSFRNKPSKDEILACWKEFR
                     GKPQELKLPSAPAQFITYFDEPDRPQTKLDRNLENGMGIAVGRLREDNLFDYKFTCLS
                     HNTIRGAAGGGILMAELLKAEGWL"
     gene            7620..>8281
                     /gene="dapA"
                     /locus_tag="PLG72_06805"
     CDS             7620..>8281
                     /gene="dapA"
                     /locus_tag="PLG72_06805"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOJ80544.1"
                     /translation="MKKRVFTGSGVAIITPFTDDGVDFDKLGELIEYQINEKTDAIII
                     CGTTGEASTMPDEEHIEVIAYTVRKVNGRIPVIAGTGSNDTRHAIHLSKRAEEVGADA
                     ILTVTPYYNKTTQEGLYRHFRAVAENIGIPVILYNVPSRTNLNIDPDTVVRLAELDNI
                     VAIKECNLNQAGEIINRCGDDFTVYSGEDALVLPMLSLGGKGVISVMANIIPHATHDM
                     VAS"
BASE COUNT         1990 a         2163 c         1862 g         2266 t
ORIGIN      
        1 gcaggcaaag ggctttgacg gtcggctctc catcgccgga cacgggattc tgtgcaagat
       61 actcttcata tatatcttct gaaaaaagct ggccggcacg ctgcagttct ctcagctctg
      121 ccagggtctc ccttgcctcg tcacgtccgt attttgctga aaacctttca atgatggcct
      181 tatccggttt atcaagttcg tcggtcatct catatgcggt atcatcgacc acatgaacgg
      241 ttccgctgtt gacgtccagt atgatgttcg taccgtgcat cctgaatttg tgtatcattt
      301 atttctcccc attctgatat catcatgtct catatttgat ctgaattggc taacacctgg
      361 gttgtccgtc gccggtatgc aataccggac acgaacatgt ccggacataa taacagcgag
      421 aggtgtatat cctcccgctg tcaaacctct tcagccggtt atctcttctc gcatttctgg
      481 tttgcgacag tgcagctcgt tttgcatgcc gactggcagg aagtctggca ttcgccgcag
      541 ccggaactgc tgatgtcttt tttgcttaca ggtgcattga tagtctttat atgtttcata
      601 tgtaatcccc ctgatctgta ataattttct gctggtcata ctttgcctga atattatagc
      661 acaatcatcg gcacatgaaa aggtcagccg tcaatatatt tatatcttgg gctcttcttc
      721 gtatttatgc tcattattcc gccgatggct cctgaaagca cgccgattat cgtcatcgtg
      781 agaacgttcc tgtctactgc aaaattgttg aacactatac tgctgaacaa ataaaggatg
      841 atcatgtaga tcagccctac aatgctcccg ttaagccaac ctttgctccg tacccccctg
      901 gtcgacaccg cgccggcagt aatcacgctt atgacagtta tgaccactac aacaggcgta
      961 accagcctct gcggaaaatc aacatttgcc agtatcagtg caaaaagcag gaatgcgggg
     1021 atagtaataa tataggaagc aagaaggcct ttaagaagca gcagcggatt gagtccttca
     1081 tactcttcct gttttatgcc ggtacttttc cttgcaggca tgttatcact cccaggcatg
     1141 tatattttat attatataat tattacctgg gttcggatta tagaactccc cggccgtcgg
     1201 ccatcttccg caaaaatgca aaaatcccgt ccttgcggct aacgggatca tatgcatggc
     1261 ttaatacagg ataactttcc tgactatgag ttcttaataa cgctcccctg gagacgtctt
     1321 ttccaggatc aggattcgat cggtttaatt acttccttta tagcccattt tttaagattg
     1381 atcttggctt tttccactcc ggtctctatc gttacctcgt catcttttat gttgacgact
     1441 ttgcccgaga taccgccgat cgttattacc tggtgcccaa cctgcatcga gtcgagcatt
     1501 tctttcgcct tcttgtccct tcttttctgg ggcaggaaaa taagcaaata catcatcagg
     1561 atgaaaaaca ccaggcaacc cagtgtacgt agccacatgc tgatgccttc agtttccata
     1621 tgcttcctcc ttcaattttt gatttattgc tacaggtctt gcaatcgacc ataataaccc
     1681 gctttaaagt ttatacccgt atgaactgaa aaattcttcc ctgaaatcac cgagtctatc
     1741 ctccattata gcttgtctta catttgccat caagtccaaa aggaatttca ggttatgcag
     1801 cgttgtcagc ctgagtccga ggatctcctg cgccttcagc aaatgcctga tatacgctct
     1861 ggtgaaattc ttgcacgcgt aacagtcgca tttgggatca agcggcgaaa agtccctggc
     1921 atactttgca tcccgcacga tgatcctgcc ttcggaagtc atcacggtcc cgttccgccc
     1981 tatcctggtc ggcagcacgc agtcgaacat gtctatgccg cgtatggccc cttccacaag
     2041 ataatcagga ctgcccactc ccatgaggta tcttggcctg tcttccggaa gcagcggtac
     2101 cgtatattcg agtatcctgt acatatcctg cggcggctcg ccgacgctga gcccgcctat
     2161 cgaatatccg gggaaatcca gctttacaag atccctagca cttttctccc tgagatcctc
     2221 atatacgcct ccctggacga tgccgaacaa agcctgcttt tcagtatcct tatgcgcttc
     2281 cctgcaccgc tctgcccatc ttgtggtcct ttccatcgat ttcttcgcgt aatcatggtc
     2341 ggccggatat ggtatgcatt catcgaatgc catgataata tcagaaccaa gatcgttctg
     2401 tatctttatt gccagttccg gtgtaaacat gtgcttagaa ccgtctatgt gagacctgaa
     2461 cgtcacgcct tcctcagtta tgttcctcag atcgctgagg ctgaatacct ggaatccgcc
     2521 gctgtccgtc agtatcggcc tgtcccagtt catgaacctg tgcagtccgc ctgcctcgcg
     2581 tatcagttca ctgcccggcc tcatgtacag atggtatgta ttgctcagga tgattccagc
     2641 cctgatctcc ttcagttcat caggagacat gcatttgacg gtggcctgtg ttccgacggg
     2701 catgaacgca ggggtctcga aggacccgtg aggcgtatgc actataccaa gtcttgctcc
     2761 tgtctgtttg cattttttaa ttagttcgta tcttatcgca gacatctgtt caccttgtcg
     2821 aaattattca tgtgatacta tattatgaac atggcgtcgc cgaagctgaa gaacctgtaa
     2881 cgctgttcga cggctattct gtaggcatca agtatatttt ccctgcctgc gaacgcactg
     2941 acaagcatga ggagcgtcga ctccggcaga tggaagttgg tgatcagccc atcaattgcc
     3001 ttgaacctgt aacccgggta aatgaaaatg tcagtcgttc ctgacgacgg ttttactaaa
     3061 ccgtcgtcag agcttacagt ttcaaggacg cggcagctcg tcgtccccac tgcgatgatc
     3121 ctgccgccct ccgtccttgc acggttgatc acatccgcag tctcactgct cagattatat
     3181 acttccgaat gcataacgtg gtcttccacg ttttctgttt ttacaggcct gaaagtccca
     3241 agtcctacat gcagcgtcag atacgcggta tatataccgc tgcggcccag atcatcgagc
     3301 agctcttttg taaaatggag cccggccgtt ggcgccgctg ccgatcccat atacctggaa
     3361 tacactgtct gatacctttc gggatcctga agcttttttg tgatataagg cggaagcggt
     3421 actatgccta ccttatccag tacctgctgg aaaatcccct catattcaaa ctggacgatc
     3481 ctgttgccgc cctcgaccac atcgaggatc tcagccttca gcaaaccgtt cccgaaaacg
     3541 aatcttgctc ctggtttggc tcttctaccg ggtttgagga tgacctccca tatatctcct
     3601 gaaatgttct tcaataatac gaattcgatg cttccgcccg tatcttccct ggcgccgaga
     3661 agccttgcgg gcattaccct agtgtcattg aggacaaggc aatcgccggg tttcaggtat
     3721 tcctttatat ccctgaagat cctgtgctca attttcccgc ttttcctgtc aagcaccatg
     3781 agcctggaac tgctcctgtc ttccagcggt tcctgggcta tcagttcctg cggcagttca
     3841 tagtaaaaat cgctggtttt catggttctc ccgtttctat gtaatgttgg cgttccatac
     3901 tttatcttct gcacatatac ataaaatatc cattaaacat cggataacga tgaaactgcc
     3961 caaataaaga gctgcaatca ctgcagcccg ttaaccgtca tccagcaaat catactataa
     4021 tttaaaccgt tttacaatat gtcagatgca actattcgac ttttgtcccc tggaagtaat
     4081 atgtcagtat ctcttcatat gtctttccgg ctttcgccat gcctatcgcg ccttcctggc
     4141 tcataccgac ggcatgtccc catcctttcc ccgtgaaggt gtaagtttcg ggtataacag
     4201 ccgccttgtt tactgcgccg tcggcgccaa gtatcgtgat cttgttgttt gagcctgaaa
     4261 tcgttgtgat cccgccagcg gtaacgacct ttttgccgcc aagctgggtc ttgattggct
     4321 ctgatgcgac aggagccgca acaacaggca atgacgttac cttttcagcc gtttccccgt
     4381 ttttaccggc ttcaggcgta ctgatttccg tttcgtcttt atcctggttt cctccattcg
     4441 ttccagcctg tccgtctgtt ccgccccggg gatcctgggt gcctgtaccg ctttccatat
     4501 caggccctga agctggaatt tcctctttcg cgatcacatt cacatcctga gcgccgtctt
     4561 tgccggcggt actatccgca gactgcatta cagggatctc tgacaggccg gcaagatata
     4621 cgtcggcatc cgtggttatc gtatataact ggctgtcgag tccgaacaaa gtacggcacc
     4681 gctcatttga aaaatacaaa ggttcccccc tgctgctgcc tgtgacggcc agttgcgtca
     4741 ctctgccggt aggcgctgtg cgggtaatcg tcacaccaag tatgctgccg agttccggaa
     4801 gcatcgattt tatatccgaa gctcgcagcg ttttcgacca tgtatatatt ttctcgtaat
     4861 catcctttac actcacaagg tacggatatg acgaccccca tacattacgt acgtcttccg
     4921 tgcttcctcc cccggaagca aaataatata catgttctat tggtttcccg ttatatgtta
     4981 taatcttgtc atacacttcg tctatggcct ttctgcattc agccacctca acgctgtagc
     5041 ctttatagac ctggcagtgg gtggtggcac atatatcgaa tccggaactt ccgtgtttcc
     5101 ccctgttgtt gagggcatac atcttggata cgacagcctg ggctttcagt gcttccgacg
     5161 gcgatttccc gcctatctca ggaggtacgt tgccgtacag atactctctg agcgaaacca
     5221 catttattac cgtcatgtca ctgttcgaaa gcctcttcac ctcgacgcag cctctgtaag
     5281 gctttccttt tatattaatg acggcagaat cagctcctgt tgccggcctt aactgcagga
     5341 aatattgctt gctcccgaaa acacacaacg tctggtattg tccgtcggta accactaccc
     5401 tgttcgaaga aggggttact atttcaaatc ccgtttccca aaacagtgac ttgagttccc
     5461 ggatcatcgc ttcggcttcg gccgtatcca tgcaaaagcc ggtccatacc tgccatgcgt
     5521 cattgtaggc gatatacgca tcaatgccgg cctggcggta tgaatgaacg ctgttcatcg
     5581 ccgatgctgc atccgggtaa tcggttccta tttttacatg ataaggaccg tatttctttc
     5641 cggtgatatt ggaacccgat ccgctgaatt cctttagact ggtgccgctc gtatggtagt
     5701 atgcgtcttt gcggacataa actgctgaag aacctgtaaa ggaacatact tccgtgaacg
     5761 tgccgccgct gttcacgaag cctgccctta caccggcctg tgccgatacg tcgaatatgg
     5821 actgggcagt atcaacagag cttcctttat aatagagccc tacccttatg gtctcttcat
     5881 ttgtattgga tgccagcccc gttcctgcat tggcggaaaa tatcactgcg aataccgcag
     5941 atgcagcaag cagtgccgtt atttttctaa tgatgctttt agccatatta tctcctcttc
     6001 attagtagaa acacctaata ataactctcc ggcgctaaaa cacctcatgt tgcctgtatt
     6061 cattttttta attcgacacg gaaagccgat cttccttctg tttcgacctt tttaccgaat
     6121 ttttcatata attttaatat ttctgtaata aaacattata agttgcggtg caaaatattt
     6181 gacaatacgt aatacggatg ttattattat taaaagcttc ttttagttgt caattgaata
     6241 cggattgata gaggcgcgct tctgatgagt aactccgttt aggcagcagg tgccgatgag
     6301 acggcgcgaa aggctgaagc gccgaaggat acgatcccct gacgggtctg gtcctgggtg
     6361 caatggcgaa cagccttgca actgtcatca ggtattgatg gagcgctatc agttctagta
     6421 agcctgacgg aaaagccagg cattagcagg catataggac tgttggcaca tcgccaacag
     6481 tttttattta tcagggaggt attggtatga actcaaaact cagagcagga gtaatcggcg
     6541 gaaccggaat ggtaggacag cggttcatca cgctgctgga gaatcatcca tggttccagg
     6601 taacggctgt cgcagccagc gaaaggtctg caggccttcc ttttgaaaag gctgtcgaag
     6661 gacgctggaa acttgatgtc ccgatcccgg aaagtgtaaa gaacatcact gtgataaatg
     6721 ccgccgatat cgacaggatg gcagatgaag tcgatttcgt tttctgtgcg gtagatatgc
     6781 cgaaggatgc tgtcagggaa ctggaagaga aatacgctcg tgcggaaata ccggtaatat
     6841 cgaacaactc tgcacacaga tccaccgagg atgttccgat gataatcccg gaagtcaacc
     6901 cgcagcacac agcgataatc gaaaaacaga gaaagcgtct cggcacgagg aaagggttca
     6961 ttgcagtgaa atccaactgc tctctccaaa gttatgtgcc gccggtccat gccctgatgc
     7021 agtttgggcc ggagaagata ctcgcttcga cttaccaggc aatatccgga gccggcaagg
     7081 tattcaggga atggcctgag atcattgaca acgtaatacc atacatcggc ggtgaagaag
     7141 aaaaaagcga aaaggaacct cttaagatct ggggaaccat cgaaggcgac gttatcaaac
     7201 cggcatcagg tcccgctgtt tccgcccagt gttacagggt ccctgtttcg gacggtcatt
     7261 tggctgcagt gtatgtgtca ttcagaaaca agccctcgaa agatgaaata cttgcatgct
     7321 ggaaggaatt caggggcaaa ccgcaggaac tgaagctccc cagcgcgcct gcacagttca
     7381 tcacgtattt cgatgaacct gacaggccac agaccaagct tgacagaaac cttgagaacg
     7441 gaatgggaat cgccgtcggg aggctgcgtg aagataatct gttcgattac aaattcacct
     7501 gtctgtccca caacactatc aggggtgcag caggcggcgg gatacttatg gctgaactgc
     7561 tcaaagctga aggatggctt tgatatcacc tgcacaaatc atgaaggagg tcgtgaatta
     7621 tgaaaaagag agtttttaca ggatcaggcg ttgccataat aacccccttc acagatgacg
     7681 gagtcgattt tgacaaactc ggcgaactta tcgaatacca gataaatgaa aaaactgatg
     7741 cgataatcat atgcgggacc accggtgaag cttcaactat gccggatgag gaacacatcg
     7801 aagtcatcgc ctacaccgtc cggaaagtca acggaaggat acccgtgatc gcaggcaccg
     7861 gcagcaatga caccaggcat gccatccatc tgagcaaacg ggcggaagaa gtcggtgctg
     7921 atgctatact taccgtcacc ccgtattaca acaagaccac acaggaaggt ttgtacaggc
     7981 acttcagggc tgtcgccgaa aatataggca ttccggtgat actgtacaat gtgccttcga
     8041 ggactaatct gaacatagac cccgatactg tagtgcggct ggcagaatta gacaatatcg
     8101 tcgccataaa ggaatgcaac ctgaaccagg caggagagat cataaaccgc tgcggtgatg
     8161 atttcaccgt ttattccggg gaagacgccc ttgtcctgcc gatgttgtct ctcggcggta
     8221 aaggtgtcat atccgtcatg gcgaacataa taccccacgc cacccatgac atggtagcct
     8281 c
//
LOCUS       DAOSCC010000104         8248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5640_length_8248_cov_4.580373, whole genome shotgun sequence.
ACCESSION   DAOSCC010000104 DAOSCC010000000
VERSION     DAOSCC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_8248_cov_4.580373"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..328)
                     /locus_tag="PLG72_06810"
     CDS             complement(50..328)
                     /locus_tag="PLG72_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOJ80545.1"
                     /translation="MFIYIALIESEEDKDKFEELYKTYKQLMFYTANNILKDVDLAED
                     AVHQAFLRIINHLDKIDEINCRKTKGFIVTIIEHVSIDIYRKRKRENG"
     gene            744..2375
                     /locus_tag="PLG72_06815"
     CDS             744..2375
                     /locus_tag="PLG72_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOJ80546.1"
                     /translation="MSRWWQERVVYQIYPRSFQDTNGDGIGDIPGIISRLDELRDLGV
                     GILWLSPVYPSPNADYGYDISDYCAINPEFGTMEDMERLIAEAAKRDIKIIMDLVINH
                     TSDEHPWFVQSRDKQSPYRDYYIWRPGKNGKPPNNWMSFFGGSTWEYDEKSGEYYLHL
                     FSKKQPDLNWRNPKVMEEVKNILRFWLDKGIAGFRCDVINLLYKTSLENGKLRPAMVL
                     PGSEHYVNQEGTHEILRTLRREVFDHYDCFTVGETVFVKTEDAKLLCGEDRRELDMVF
                     FFEHMETDAVAKWIRRKFDPARFVRVISRWQDALEWNANYLENHDQPRSVSRFGDDGR
                     YHAESAMMLGTLLLTLRGTPFIYQGQEIGMTNFDFTGMEQIRDVESHNVYNLLKRLHI
                     PSSLRWKIIKPTSRDNARTPMQWDNSPNAGFTTGTPWIGVNGNYTRINVASQMNDPGS
                     IRSYYKKLIVLRNSSKTLIYGDFKPLEITRRLFAYRRELDGHSITVLLNFSDKPQKAD
                     FSGRLVMSNYGRSSYGGTLEPYEAVLLENGRMVQA"
     gene            2372..4714
                     /locus_tag="PLG72_06820"
     CDS             2372..4714
                     /locus_tag="PLG72_06820"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ80547.1"
                     /translation="MIEYQDGVFRLSTQNTSYWFRVTKFGHLEHIHYGPRLKEQPVEG
                     LLLKRTAAIGSSVSYDESDPTYCLDNMCLEWSGIGRGDYRHSPAELKMPDGTFTCDFL
                     YKSHRILPGHVSMDPLPTAYGGENECRTLELTLEDEPNSCRLILYYTVYEKTNVITRR
                     AVLENHSDKPLVIRRLMSMMVDIPDRGFRLVTFDGGWIKEAHRHERPLQYGIFINSST
                     TGASSNRHNPGFLLAEEGATQEHGHVYGFNLVYSGNHYGAVELSNHDIVRVMQGINPH
                     CFEWTVQKGESFCTPEAVMTFSPDGYNGLSHNFHDFINNHIVRGEWKSKERPVLINNW
                     EAHFFKFTQGRLLRLARRARRLGIELFVLDDGWFGDRNNDKAGLGDYNVNRKKLPRGL
                     AHFAGKIRKMGMEFGLWFEPEMVNPDSGLFRNHPEYAVTTPGRKPTMGRNQLVLDLCN
                     PAVRDYIVDSVGRILDEAGITYVKWDMNRHISDAYSPHIRSQGEFFHRYILGLYDILE
                     RIFRPRPHILLESCSSGGNRFDLGMLCYSPQIWTSDDTDPAERLKIQDGLSYLYPLST
                     MGAHVSSAPHQQTLRDTPLATRFNVACFGCLGYELDLKFLSWAERREIKDQIAFYKKH
                     RRTFQYGRFYRLPSVKDNKVYWQVVDPGRDKAIAGLFQTRAEASEGYDFLPLTGLDPD
                     KCYSLSTKPQSLFLKRFGGLVNHLLPFTLDPNGFILRTAGKYYCLTDCVEKYIGFGDL
                     LMSGVRLSNQFMGTGYNSRIRLLGDCGSNLYVIEKNEPPG"
     gene            4976..6352
                     /locus_tag="PLG72_06825"
     CDS             4976..6352
                     /locus_tag="PLG72_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOJ80548.1"
                     /translation="MKTTASRWNRYTFGLGTVGRDMLYSLVSMYLIFYLTDILRLPDS
                     MLWWLTAILLIARIFDAVNDPIMGVIVDNTDSRFGKFKPWIAAGALFTGVFTVMMYTD
                     FGLDGAPYLIMFTVIYLLWDVSFTANDIGYWSMLPSLSMDQKEREKIGAFARICADVG
                     LFAVVVGIVPVTNALGSALGSMKKAYFVFTVIISLLMIGGQCITLFGVKEFKGVFKKE
                     EQTTLGGMVRAIFKNDQLLFTVIAMALFMIGYSTTTSFGLYYFKYAYGDENMYPVFAL
                     VLGVSQIIALAVFPLFRKKFERKALYAGATALVVAGYMIFFFSPMDMRFIGAAGVLVF
                     LGEAFIQILMLMFLSDTIEYGQWKLGRRNDSVTLSLQPFINKIGNAIASGIVGATVII
                     SGISQAASAADVTEEGLVILKAAMFILPLICILAGYVVYRLKYKIDSKMYSQILADLQ
                     ARGDINLE"
     gene            6427..7389
                     /locus_tag="PLG72_06830"
     CDS             6427..7389
                     /locus_tag="PLG72_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOJ80549.1"
                     /translation="MTIVLTIFINNILPIFLLVGLGYIFGRKLGPDINTLTKLNLYAL
                     VPVFTFVYLYTSDIPADMLKVLAFAVIMLAACAALSLAVSRIMGFDQGKKYAFINSVI
                     FYNSGNIGIPLITLVFSSAPFIIDGKTPYLELALTSQIMILVVQNITTNSIGFYNAGR
                     AKMDWRQSLKKILGVPTIYAVPAAILLKLVPYDLTRLPVWPSLDYMRNALVAVALLTL
                     GVQIFRTKFNKLDKGVILSAFIRLVCGPAIAFVVLRLLSLDGVIAQAVLISSGVPTAV
                     NTALIAVDYDNCPGFASQTVLLSTLLCTLTLPAVIYAARILFPV"
     gene            7678..>8248
                     /locus_tag="PLG72_06835"
     CDS             7678..>8248
                     /locus_tag="PLG72_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ80550.1"
                     /translation="MEKTFDTSRKFADLGIIPPILKALGEMGFEYPTEIQSRAIPHAL
                     ARKDIIVMAKTGSGKTAVFGVSMLQLTDPEAEGPQGLILTPTRELAVQVDSDIRQMAK
                     FLAHRTAVVYGQHSMEAEVKELEKGATIVTGTPGRVYDHIQQGTLRTRNIKFLVLDEA
                     DRMLDMGFIDQVKRIIRSVPRDRVTALFSA"
BASE COUNT         2111 a         2157 c         2099 g         1881 t
ORIGIN      
        1 aacgaaggga agataaagta gaaatcattt tacaccactt gacaagacac tatccgtttt
       61 ctcttttacg tttcctgtat atatcaatag aaacgtgttc aattattgta acaataaatc
      121 cttttgtttt gcggcaatta atttcatcta ttttatccag atgattgatg atacgcaaaa
      181 aagcctgatg tacagcatct tctgccagat ccacgtcctt taatatattg ttggctgtat
      241 aaaacataag ctgtttatag gtcttgtata attcctcaaa cttgtcttta tcttcctcgc
      301 tttcaatcaa tgcaatataa ataaacactt atctatctct ccacatcatt tatgccatat
      361 gaaaatccat atatgcttac attattttca attatatcag acatgaatat cttgcgaaat
      421 gtttcattat aaaagaaaaa aggaaatgaa tgttacatga catccagacg aaatctgcgt
      481 atctataata taaacaatat ctgtctatga attgggattg atccatatgc tgctgtactt
      541 gcccgaatcg tcgcagagtt catcctcgat catgttcatg aaagactata gtaaattatc
      601 catatgcgac taaatctttc cacatggcag ccgtctaaca tgatataata aattgtacgg
      661 atcatacggc agaagaacct tccaggatca ctaaaacgta tagttgcatt tcatgcccaa
      721 attaaccgca aggagtggtg tttatgtcca gatggtggca ggaacgcgtt gtgtaccaga
      781 tctatcccag gagcttccag gataccaacg gagacggtat cggcgatatc cccggcataa
      841 tctcacgcct ggacgagctt cgtgacctgg gcgtcgggat actttggctc agccccgtat
      901 atccttcccc caatgccgac tatggctacg atatcagcga ttattgcgcc ataaatcccg
      961 aattcgggac catggaggac atggaacgac tcattgccga agccgccaaa cgggatataa
     1021 agataatcat ggacctggtt ataaaccaca cttccgatga acatccatgg ttcgtacaaa
     1081 gccgtgacaa gcagagcccg taccgggatt actacatctg gaggcccgga aaaaacggga
     1141 aacctccgaa caactggatg agcttcttcg gcgggagtac ctgggaatac gatgaaaaaa
     1201 gcggcgaata ttacctgcac cttttctcga aaaagcagcc ggacctcaac tggagaaacc
     1261 cgaaggtcat ggaggaagta aagaacatac tgcgcttctg gctagacaaa gggatcgcgg
     1321 gcttcaggtg cgacgttata aatttgcttt acaaaacgag cctggaaaac ggaaagctaa
     1381 ggccggcgat ggtgctgccc ggcagcgagc actacgttaa ccaggaaggc acccacgaga
     1441 tcctgaggac gctacggcgg gaagtattcg accattatga ctgcttcacg gtcggggaaa
     1501 cggtattcgt gaaaacggaa gatgcaaaac tgctgtgcgg cgaggaccgc cgggaactcg
     1561 acatggtctt cttcttcgag catatggaaa ccgatgcggt tgcaaaatgg atacggagga
     1621 aattcgatcc cgcccggttc gtacgggtca tctccaggtg gcaggatgcc cttgaatgga
     1681 acgccaatta cctggagaac catgaccagc cccgcagtgt ctcgcgtttc ggagatgacg
     1741 gccggtacca tgcagagtcc gccatgatgc tcggcactct tttgcttacc ctgcgcggaa
     1801 cgcctttcat ataccagggc caggaaatag ggatgaccaa cttcgacttc accggaatgg
     1861 aacagatccg cgacgtggaa tcccacaatg tctacaatct tctcaaacgg ctgcacatcc
     1921 cttcttcatt gcgctggaag atcataaagc ctacatcgag ggacaatgcg cgcacgccga
     1981 tgcagtggga caacagcccc aatgccggtt ttacaacggg cacgccctgg atcggcgtga
     2041 acggcaacta cactcggata aacgtggcgt cccagatgaa cgatcctggt tccatccgga
     2101 gctattacaa aaaactgatc gtcctgagga actcgagcaa aaccctgata tatggcgatt
     2161 tcaaaccatt ggagatcacc cggcgccttt tcgcgtacag gcgggaactg gacggacact
     2221 cgataacggt ccttctgaat ttctccgata agccgcagaa ggcggacttc agcggccgcc
     2281 tggtcatgtc gaactatggc aggtcatcat acggaggcac gcttgaacca tatgaggcgg
     2341 tgttgcttga aaacggaagg atggtgcagg catgatcgaa taccaggatg gtgtgttccg
     2401 gctgtcgacg caaaatacct cgtactggtt ccgggtgacg aaattcgggc acctggaaca
     2461 catccactat ggcccgaggc tgaaggaaca gcctgtcgaa gggcttctgc tcaaacggac
     2521 cgctgcgatc ggaagcagcg tatcctacga cgagtccgat ccgacttact gcctggacaa
     2581 catgtgcctt gaatggtcgg gcatcggccg cggcgactac cgccactccc ccgcggaact
     2641 taaaatgccc gacggcacct ttacctgcga ttttctctac aaatcccaca ggatattgcc
     2701 gggccatgtc tccatggatc cgctgcccac cgcttatggc ggagaaaacg aatgccgtac
     2761 gctggaactg acacttgaag atgaacccaa cagctgccgg ctgatccttt actacaccgt
     2821 atatgaaaaa accaatgtca taacacgccg ggcggtactg gagaaccata gcgacaaacc
     2881 gctggtcata cgcaggctga tgagcatgat ggtggacatc cccgaccgcg gtttccggct
     2941 tgtcacattc gacggcggct ggataaagga agcgcaccgc catgaaaggc cgctgcagta
     3001 cggcatattc ataaactcct cgactaccgg cgccagcagc aacaggcaca atcccggttt
     3061 tctgctcgcc gaagaggggg cgacccagga gcacggccat gtctacggct tcaacctggt
     3121 gtacagcggc aaccactatg gcgcggtgga actgagcaac cacgatatcg tcagggtcat
     3181 gcagggcatc aatccccact gcttcgagtg gactgtgcaa aagggagaat cgttctgtac
     3241 tccggaagca gtcatgacat tcagtccgga cggatacaac ggcctcagcc ataacttcca
     3301 cgatttcata aacaaccata tcgtaagagg cgaatggaaa agcaaagaac ggcccgtact
     3361 gataaacaac tgggaagccc atttttttaa gttcacccag ggcaggctcc tgaggcttgc
     3421 acgaagggcc aggcgcctgg gtatcgaact gttcgtgctc gacgacggct ggttcggcga
     3481 ccgcaacaac gacaaagccg ggctcggcga ctacaatgtg aacaggaaaa agctgccccg
     3541 gggccttgcg cactttgccg ggaagataag aaagatgggg atggaattcg ggctctggtt
     3601 cgagcctgaa atggtcaacc cggacagcgg cctcttcagg aaccacccgg agtatgccgt
     3661 cacaaccccg ggcaggaagc ccacaatggg ccggaaccag cttgtccttg atctctgcaa
     3721 ccctgccgtg agagattaca tagtcgactc agtcggcagg atcctggacg aggcgggcat
     3781 aacctatgtc aaatgggata tgaaccgcca cattagcgat gcgtattccc ctcacatcag
     3841 gagccagggc gaatttttcc acaggtacat actcggcctt tacgacatac tggaacgcat
     3901 tttcaggcca cgtccccaca tactgctcga aagctgctcc agcggcggga accgcttcga
     3961 tctcgggatg ctgtgctatt cgccgcagat atggacgtcg gacgatacgg accccgctga
     4021 aaggctcaaa atacaggacg gcctttctta cctctaccct ctttcaacca tgggagcgca
     4081 tgtttcaagc gcgccgcacc agcagacgct gcgtgacacg ccgcttgcca cccgtttcaa
     4141 cgtggcctgc ttcgggtgcc ttgggtacga actggacctg aaattccttt cgtgggctga
     4201 aaggcgtgaa ataaaggatc agatagcatt ttacaaaaag caccgcagga cgttccaata
     4261 cggaagattc taccgcctgc cgtccgtgaa ggacaacaaa gtgtactggc aggtcgtcga
     4321 tcccggcagg gacaaggcca ttgccggcct gttccagaca cgggcggaag ccagtgaagg
     4381 gtacgacttt ctgccactga ccgggctcga ccccgacaag tgctattctc tgtccacaaa
     4441 accccaaagt ttgttcctga agcgcttcgg cgggctggtc aaccatctgc tgccgttcac
     4501 tctcgacccg aacggcttta tcctcaggac tgccggcaag tactactgcc tcactgactg
     4561 tgtcgaaaag tacatcggct tcggggacct gctcatgtcg ggagtacgcc tgagcaacca
     4621 gttcatgggc acagggtaca actcccggat caggctgctc ggcgactgcg gctcgaacct
     4681 gtatgtcata gaaaaaaacg agcctccagg ctgagccggc cgtcagatgt cccgtttatc
     4741 tgcaggccgg catgtatccg tttccattag tgattttgca ggcgcgatga tgaaataatg
     4801 cgataatgat ataattgaat tagtccggaa gtttgaatca cagcttaagg catcacgatc
     4861 cggtcaaggc agaagtaact ttcaaaacct ggccgcagaa acttcagtat ccggaccctg
     4921 acagaagttc acaaaccggc cggtactata tcctaaagcg aatggaggcc actttatgaa
     4981 aaccaccgct tctcgttgga accgctacac cttcgggctc gggaccgtcg gaagggacat
     5041 gttgtattcg ctggtaagca tgtacctgat tttctacctt acggatatcc tccggctgcc
     5101 ggacagcatg ctctggtggc ttacggcgat actgctcatc gcgcggatct tcgacgccgt
     5161 caacgatcct atcatggggg tgattgtcga caacacggac agcaggttcg gcaaattcaa
     5221 gccgtggatc gcggcaggcg cactgttcac cggtgttttt acggtgatga tgtacacgga
     5281 tttcggtctt gacggagcgc cttacctaat aatgttcacg gtcatatacc ttctctggga
     5341 tgtcagcttt accgcgaacg acatagggta ctggtcgatg ctgccgtcgt tgagcatgga
     5401 ccagaaagag cgggaaaaaa tcggggcctt tgctcgaatc tgcgccgacg ttggcctttt
     5461 tgccgtggtc gtcgggatag tgccggtcac aaatgccctc ggcagtgcgc tcgggagcat
     5521 gaaaaaagca tatttcgtgt ttaccgtgat aatttcgctg ctgatgatcg gcggacagtg
     5581 cataacgctg ttcggcgtga aggaattcaa gggcgtgttc aaaaaagaag agcaaactac
     5641 cctgggcggg atggtgcgcg ccatattcaa aaacgaccag ctcctgttca ccgttatcgc
     5701 catggcgctg ttcatgatcg gctattcgac caccaccagc ttcggcctct actactttaa
     5761 gtacgcctat ggtgatgaaa acatgtaccc ggtattcgca cttgtcctcg gcgtttcaca
     5821 gatcattgcc cttgcagttt tcccgctgtt caggaaaaaa ttcgagagga aagcacttta
     5881 tgcaggtgcg accgcccttg tcgtcgccgg atacatgatc ttcttcttct cccccatgga
     5941 catgcggttc ataggcgccg ccggagtcct ggtattcctg ggtgaggctt tcatacagat
     6001 ccttatgctg atgttcctgt ccgacacaat cgaatacggg cagtggaagc ttggcaggcg
     6061 caacgacagc gtcacgctct cgctgcagcc cttcataaac aagatcggca atgcgatagc
     6121 cagtgggata gtgggcgcaa cagtgataat ttccgggata agccaggcgg cgtcagcggc
     6181 cgatgtcacg gaagaaggcc tcgttatact taaagccgcg atgttcatac tcccgctgat
     6241 atgcatactc gcaggctacg tggtctacag gctcaaatac aaaatagaca gcaaaatgta
     6301 cagccagatc cttgcagatc tgcaggcacg gggcgatatc aacctggaat gatgatgttg
     6361 ttgcaaaaca tgcagtattg gattaaggta aaataaacta cagataacga cggaaggaat
     6421 gtacaaatga ccatagtact cacgatcttt ataaacaata ttttgcccat attcctgctg
     6481 gtgggcctgg gttacatatt cggacggaaa ctcggcccgg acataaacac cctgacgaag
     6541 ctgaacctct atgcgctggt tccggtattc acattcgtct atctctacac gtcggatatt
     6601 ccggcagata tgctcaaggt gcttgccttt gccgtcatca tgctggctgc ttgtgccgct
     6661 ctgtcgctcg ctgtttcccg tatcatgggg tttgaccagg gcaaaaaata tgccttcatc
     6721 aattcggtaa tattctacaa cagcggcaac atcgggatcc cattgataac actggtattc
     6781 agcagcgctc ctttcatcat cgacgggaag acaccatacc tggaactggc acttacatca
     6841 cagatcatga tccttgtagt acagaacatc acgaccaatt ccatcggctt ttacaatgcc
     6901 gggcgtgcca aaatggactg gcgccagtct cttaagaaga tcctgggcgt acctactata
     6961 tatgccgttc cggcagccat cctgctgaaa ctggttccat atgacctgac acggctgcca
     7021 gtatggcctt ccctcgatta catgcggaat gctcttgtcg cagtagcctt actgacgctg
     7081 ggcgtgcaga tattccgcac gaaattcaat aaactggata aaggcgttat cctgtccgca
     7141 tttattcgcc ttgtctgcgg accggctata gcgtttgtgg tattacggct gctgtcactg
     7201 gacggtgtaa tagcacaggc agtgctgatc tcatccggtg tgccgaccgc agtcaacaca
     7261 gcgctgatcg cagtggatta tgacaactgt cctggttttg cttcacagac cgtgttgctt
     7321 tccacgctgc tttgtacgct gaccctgccg gctgtgatat atgccgcaag gatcctgttc
     7381 cctgtctgaa gccggcctgg tcccggctga cccaaacccg gattaatacc agttgatccc
     7441 ggaccggctc agattattgc tggtcacatc tggttttggt cttcgttggc cccgaatgga
     7501 attcttgttc gtccggacct gatcccttat ccgtcatggc cctgatctct ccggtccctg
     7561 ttccatgact gcttccgcat ccggggatca cacgcggtca aggtggaata tgccgcagta
     7621 tagtgctata atgctataat gaaaaccgaa ggaaatcatg aatgagagaa gatgcaaatg
     7681 gagaagacat tcgatacaag cagaaaattt gctgatcttg ggatcatacc tcccatattg
     7741 aaagcgctgg gagaaatggg attcgaatat ccgacagaga tccagagccg ggctattcct
     7801 cacgctttgg ccaggaaaga cattatagtt atggccaaga ccggaagcgg caaaacggca
     7861 gtgttcggag tatcgatgct gcagttgacc gatccggaag ctgaaggccc gcaggggcta
     7921 atactgacgc ctacccgtga gcttgccgta caggtggaca gcgatatccg ccagatggca
     7981 aagtttcttg cccacaggac agcggtcgta tacggccagc acagcatgga ggccgaagta
     8041 aaagagcttg agaaaggcgc aacgatagtc acagggactc cgggaagagt ttacgaccat
     8101 atacagcagg ggaccttgcg tacaagaaac ataaaattcc tggttctcga cgaagcggac
     8161 aggatgctcg acatgggctt tatagaccag gtgaaaagga taataaggtc tgtgccgcgt
     8221 gacagggtca cggcgctttt ttccgcca
//
LOCUS       DAOSCC010000105         8220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5661_length_8220_cov_3.808328, whole genome shotgun sequence.
ACCESSION   DAOSCC010000105 DAOSCC010000000
VERSION     DAOSCC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_8220_cov_3.808328"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..812)
                     /locus_tag="PLG72_06840"
     CDS             complement(81..812)
                     /locus_tag="PLG72_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80551.1"
                     /translation="MGKELWKTKSFNFTSWNAAKTDIEEAKKQIDSMVSGCSVNTVAI
                     TFAARQDHCYSTEIHWKGSHMFDEPKLAELITHAKNAGLRVIVKPMLNVSDGYWRAYI
                     RFFDEDVPCEPKWHDWFREYTDYIVHYARLCDRYGADMLIIGCELVGTDHRHREWRRL
                     IESVRSVYSGLLSYNCDKYQEHRVTWWDALDAISSSGYYPINDWDRQLARIYDTVTIY
                     NKPFFFASGFKIKIKAGLIISHFSF"
     gene            1019..1105
                     /locus_tag="PLG72_06845"
     tRNA            1019..1105
                     /locus_tag="PLG72_06845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Leu,seq:caa)
     gene            1377..2330
                     /locus_tag="PLG72_06850"
     CDS             1377..2330
                     /locus_tag="PLG72_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HOJ80552.1"
                     /translation="MGKKILFFACLAVIAMTCSVAVVLSIVRYGPDESADAGYDTEVV
                     RTDRATTDSGDRQENDVSGIPAECESTQQVDVKDDGGGVVSEDSQNIQVEKNRERGED
                     HDTVPVPVICGKKDDLYDADGNTEGNIVTTGTADGITVIFKPDLPQPRGDMDSQGTTF
                     IIKPKEVIKLDPSLADKLRKHGEEKGKEASGLFRTMLAVEQTEEGLVLKFSLENISGT
                     DLEYYSGSSQKYDYSIYDSNGNEVYRWSNDKDFLAVITEMLLRKGEKASFREIWDYTG
                     NDGKRVLHGEYSVVVRMIAKLNDGRKTDPGELTAKAYFTVD"
     gene            2585..3004
                     /locus_tag="PLG72_06855"
     CDS             2585..3004
                     /locus_tag="PLG72_06855"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOJ80553.1"
                     /translation="MYFEWREEYRTGIDAIDKQHRHLLEIGAKIFDLANTDDGYDHYD
                     EIMEVLDELKDYTVYHFGYEEDLMERYGYERYEPHKFQHYFLTKKIEKFDKERYDIDE
                     KQNEAVLKLAEFISDWVTNHILGEDMQYKDFFKSKGL"
     misc_binding    3075..3334
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3414..5789
                     /locus_tag="PLG72_06860"
     CDS             3414..5789
                     /locus_tag="PLG72_06860"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ80554.1"
                     /translation="MDKKYSFERTEKEMQEFWEENGIYRFDPEKPGDIWSIDTPPPTV
                     SGSLHIGHVFSYAQAEMIARYKRMRGFNVFYPFGFDDNGLPTERLVEKQEGVKAGSMP
                     RKRFVEMCLTTTSKYIEEFRELWQSLGFSVDWSLQYETIEPKAQKISQRSFIKLFREG
                     KAYMRESPVLWCTDCRTSIAQAELETAEKETSFNYIPFETAEGDEKLIIATTRPELLY
                     GCVSLFVNPNDERYARYEGRTVRVPLYGHEIPVLSDRKVAMDKGTGIVMCATFGDSTD
                     VEWFEEHRLPYRKTITQDGTIDLSVPYIGGMEIKEARKHIIGLLKEKGLIARSEELTH
                     TVAVHERCGKETEIILSRQWYIDILSNREKCLEAAEQINWYPDYMKERYRAWVENLKW
                     DWCISRQRYFGVPLPVWYCSRCGKPAAADESQLPVDPAESRPGRPCSCGSTDFTPETA
                     VLDTWATSSVTPQINARWGEADDISERLLPMSMRTQAHEIIRTWAFYTIVKSLYHMGR
                     IPWKDIMICGFVLAKKNEKISKSKSNSAMSPKLLTEKYSADAIRYWAANSRLGTDTFF
                     SLDDLDISMRFITKLWNAARFSISRLQDFDPSEEPELLPVDRWIRERCKQTMHEAGKL
                     LDQYEVGLARHVIDDFFWNDFCDDYIEIVKERLYQPDIHGHPERRSAQRALYDSLFDI
                     LKMYAIYVPHITEYIYQVYFRQHEDEISLHLTRWEDAEEFDYDILEFGRQLKEAVSRA
                     RRIKSARSLSMKAEIEGLDIKTEEKYKEYFIRSIPDIRACCRAREVKLSIK"
     regulatory      5926..6028
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            6147..6665
                     /locus_tag="PLG72_06865"
     CDS             6147..6665
                     /locus_tag="PLG72_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOJ80555.1"
                     /translation="MKKTKVIVFMSILTALNVLLTHVVPLITTDFVRISFGFIPLSLG
                     AMLFGPVIGGIGGAVSDLLGMVIAPKGPYFPGFTLSAFLSGVIYGLFLYRKPKNLGRI
                     TLAVLCVSIFVNLGLNTLWLTMLQGKGFLALLPARALQNAVTSVVQIVLIPLVWKYAG
                     RSIENEYLHQPV"
     gene            complement(6725..7048)
                     /locus_tag="PLG72_06870"
     CDS             complement(6725..7048)
                     /locus_tag="PLG72_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOJ80556.1"
                     /translation="MNEKKSRNLDYLDKGVAISPAVPVVGDKITILYDGSLAKNGASE
                     ITVHLGYGSNWDNEQDIPMVKKDTCFEATIPALKEDFLKLSFHDPFNNMDDNNGQGYS
                     FDIMK"
     gene            7261..>8220
                     /locus_tag="PLG72_06875"
     CDS             7261..>8220
                     /locus_tag="PLG72_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOJ80557.1"
                     /translation="MKAVIMAGGEGTRLRPLTCNRPKPMVPVAGRPVMEHIVRLLKTH
                     GIDDVAVTLQYLPDHIMEYFGDGSDFSVRMRYYIENKPLGTAGSVKNAEDFLDETFIV
                     ISGDALTDIDLTKAVSFHREKGAMATLVLKRVDVPLEYGVVVTDGDGKIIRFLEKPGW
                     GEVFSDTVNTGIYILSPEVFDYYERDQVFDFGRDLFPLLMKENRIMYGYITDDYWCDI
                     GDINAYIQANFDAMDKKVKVTVPGIEIRRGVWTGRGTVIDESASIEGPCIIGDCCRIK
                     GGAVIGSHTVIGDNCVVEENGSIKRSIIWKNSIIGEKAEIRGSV"
BASE COUNT         2302 a         1720 c         2164 g         2034 t
ORIGIN      
        1 aaggtttgca aaacgccacc tccccttaaa gagaagggct aaaagaaaac aaaattaagg
       61 aaataacaga tatcactgtt ttaaaaagag aagtggctta tgatcaatcc cgctttgatc
      121 tttattttga aaccagacgc aaagaaaaag ggtttattgt atattgtgac cgtatcatat
      181 atccttgcaa gctggcggtc ccagtcattg atcggatagt aaccggagga agaaatggcg
      241 tcaagggcgt cccaccatgt gacgcggtgt tcctggtatt tgtcgcagtt gtatgaaagc
      301 aagccgctgt atactgaacg tacgctttct ataagacggc gccattccct gtgcctgtgg
      361 tctgttccaa caagctcgca gcctattata agcatgtctg ctccatacct gtcgcaaagc
      421 ctcgcataat ggacgatgta atcggtatat tccctgaacc aatcgtgcca ttttggttcg
      481 catggcacat cttcgtcgaa aaagcggata tatgcccgcc agtacccgtc cgacacattc
      541 agcattggct tgacaatgac cctgagcccg gcgtttttcg catgtgtgat caattctgcc
      601 agcttcggct catcaaacat gtgggaacct ttccagtgaa tctcggtgga atagcaatgg
      661 tcctgccttg cggcaaaagt aattgcaacg gtattgactg aacaccctga aaccattgaa
      721 tcgatctgtt tttttgcttc ttcgatgtcg gtttttgctg cattccatga agtgaaattg
      781 aatgattttg ttttccacaa ctctttgccc ataaacgtac cctctgatag atcgttaaca
      841 tcagtataaa taatgcgcac ggtcttttca acagaagaag aagaaacgcg aacgggtata
      901 cccggaaacg catgcatgaa atgagactca tgtgcagtgt acgagatgtt ttgcaggatt
      961 ctgtttcttg acaacatcat gtgcctgttg tataattcaa gtgcagtggc ggaagcatgc
     1021 cgaagtgatg gaattggcag acgtagtgga ctcaaaatcc accggtagca ataccgtgcc
     1081 ggttcgagtc cggccttcgg caccacagga aagagcaagg tttggaggca cagatgcagt
     1141 gaacagacct tgctcttttt tatgattacg gcactttttg acatttgcgt cagtttaacg
     1201 ctgccccttg ttgatatctt cacctccgga acaaatgatt gcttctttcg tctatagatc
     1261 cggatgacat ttcacctgaa agtacagtat tagcggatgc ataatatttt tctggcgtca
     1321 ggctgataca actataatca gattgctgac tgcttcattt acagggagtg atgtttatgg
     1381 gtaaaaagat tctgtttttt gcttgcctgg ctgtgattgc catgacctgt tcggtagcag
     1441 tggtcctcag catcgtaaga tacggcccgg atgaaagtgc cgatgcagga tacgacacgg
     1501 aagtcgtccg gactgaccga gcaaccacgg acagtggaga ccggcaggaa aatgacgtat
     1561 caggtatacc tgcagaatgt gaaagcacac agcaagtcga tgtgaaagat gatggaggcg
     1621 gcgtcgtatc cgaagatagc caaaacatcc aggtcgaaaa aaaccgggaa agaggtgaag
     1681 accatgatac tgtgcccgtg ccggtcatct gcggcaaaaa agatgatctg tatgatgccg
     1741 atggaaatac tgagggaaat atagtcacaa ctggcactgc tgacggcatc actgtaatat
     1801 tcaagccgga tttgccgcaa ccgcgcggtg atatggacag tcaaggaact acatttatta
     1861 taaagccaaa agaagtaata aaacttgatc cttcattggc ggataaactg aggaaacatg
     1921 gggaagaaaa gggaaaagaa gcatcaggtt tgttcaggac aatgttggct gttgagcaga
     1981 ctgaagaagg actggtgctg aaattttcac tggaaaacat ctctgggacg gatctggaat
     2041 attattccgg ctccagtcag aagtacgact attccatata tgacagcaac ggcaatgaag
     2101 tatacagatg gtcaaatgat aaagacttcc tggcggtcat aacagagatg ctgctcagaa
     2161 aaggtgaaaa agcatcattc agggaaatat gggattatac cggcaatgac ggcaaaaggg
     2221 tcctgcatgg agagtattca gttgtggtga ggatgatcgc gaaattgaac gacggaagga
     2281 agaccgatcc gggagaactg accgcaaagg catatttcac tgtagactaa aggcatattt
     2341 cacgtcatcc ggcgcggagc aaagcagggt acggcttgca atacactgaa acagctgcat
     2401 tttcgctgcc gtgaccgctt actgttcata acagtattgc ccttttgaaa aaatctgctg
     2461 tcaaccggtt tataatcatt ttttcagtgg tatatttaga ccatagtcaa gatttgtcaa
     2521 aatcctgcat gcagacaagc gagatgtttt gcagagagtc tgatttaaca gagggggttc
     2581 ctgaatgtat ttcgagtggc gagaagagta tcgtaccggg atcgacgcga tcgacaagca
     2641 gcacaggcat ttattggaaa taggcgcaaa aatcttcgat cttgcaaata ctgacgatgg
     2701 ttacgaccat tatgatgaaa taatggaagt cctggatgaa ctgaaggatt acacggtata
     2761 ccatttcggc tatgaggaag acctgatgga gcggtacggc tatgaacgct atgagccgca
     2821 caagttccag cattatttcc tgaccaaaaa aatcgagaaa ttcgataaag aaagatatga
     2881 catcgatgaa aagcaaaatg aagcagtact caaactggcc gagttcattt cggattgggt
     2941 aacgaaccat atcttggggg aggatatgca atataaggat tttttcaaga gcaaaggcct
     3001 ctgacggttt ttgaagggat cgtattgaca attgtaaacg tctggtctat cattataaaa
     3061 aaacgaacac aggcaaaacg gcgttgaagg ggaggagtac atacgctcct gaaacacaga
     3121 gagaagatgg atggtgcgaa tcttcatgac ggacgtgtgg aagtagcccc tgagctgtgg
     3181 acccaaaggc atgcgccgag taggctccaa cggaaatttc caccgttaaa gggaaagcaa
     3241 tatcggatat ccctgcatga tatcccgtat tggcagagtg cagatctatg atctgaatta
     3301 aggtggtacc gcggatcctg agtccgtcct gagcatgacg aaatgtctgc ttggggcgga
     3361 ctttttgatt tccgtaagtt ttgattgtgt tcgggaacga ggaggtatca tcgatggata
     3421 aaaagtactc atttgaacgg acggaaaaag agatgcagga attctgggaa gaaaacggca
     3481 tttaccgttt cgatcccgaa aaacccggag atatctggtc tatagatact cctccgccga
     3541 ctgtgagcgg cagcctccac atagggcatg tcttttcata tgcccaggct gagatgatcg
     3601 ccagatataa gcggatgcgt gggtttaatg tcttttatcc tttcggtttt gatgacaacg
     3661 ggctgccgac cgaaagactc gtcgaaaagc aagaaggcgt aaaagccggc agcatgccaa
     3721 ggaaacggtt cgttgaaatg tgcctgacga ctacgtcgaa atatatcgaa gagttcaggg
     3781 aattatggca gtcgcttggc ttttccgttg actggtcgct ccagtatgag accatagagc
     3841 cgaaggcaca gaagatatca caaaggtcat tcattaagct gttcagggaa gggaaagcat
     3901 acatgaggga gtcgcctgtg ctctggtgca cggattgcag gacttccata gcacaggctg
     3961 aactggaaac tgcggaaaag gaaacgagtt tcaactatat accctttgaa acagctgaag
     4021 gagatgaaaa actcatcatt gccactaccc ggccggaact gttgtatggc tgcgtcagcc
     4081 tgttcgtcaa ccctaatgac gaaaggtatg ccagatatga aggaaggacc gtccgtgtcc
     4141 cgctgtatgg ccatgagatc ccggtgcttt cggacaggaa agttgcgatg gataaaggca
     4201 ccggcatagt tatgtgcgcg actttcggag acagtaccga tgttgagtgg tttgaagaac
     4261 atagactgcc gtacaggaaa acgataacac aggacggaac gatcgacttg agcgttcctt
     4321 acatcggcgg aatggaaata aaggaggcaa ggaagcatat catcggactg cttaaggaaa
     4381 aggggctcat agccaggtcc gaggaactca cgcatacggt ggctgtccac gagcgatgcg
     4441 ggaaagaaac ggaaatcata ctttcaaggc aatggtatat cgatatattg tcaaacaggg
     4501 aaaaatgcct tgaagcagca gagcagatca actggtaccc ggattatatg aaagagaggt
     4561 acagggcctg ggtcgaaaac ctcaagtggg actggtgcat ttccaggcag cgctatttcg
     4621 gcgtacctct tcctgtctgg tactgcagcc ggtgcggtaa acccgcggcg gcagacgagt
     4681 cgcagcttcc ggtcgatccg gccgaaagca ggccgggcag gccatgttcc tgcggaagta
     4741 ctgattttac accggaaact gccgtcctcg acacttgggc tacatcttcc gttacgcccc
     4801 agatcaatgc ccggtggggc gaagccgatg atataagcga aaggctgctg cctatgagta
     4861 tgcggacaca ggcacatgag atcatacgga catgggcttt ttacacgata gtcaaaagcc
     4921 tttaccacat gggccggatc ccatggaaag atatcatgat ctgcggtttc gttcttgcaa
     4981 agaaaaatga gaagataagc aaatcgaaaa gcaactctgc catgtctcct aagcttctta
     5041 cagagaagta ctcggctgac gccatcaggt actgggcggc aaattcaagg ctcgggaccg
     5101 acactttctt ctcccttgat gatctggaca tctccatgag attcataacg aagctgtgga
     5161 atgccgcaag gttctcgata tcacggctcc aggacttcga tccatcggag gagccggaat
     5221 tgcttcctgt ggacagatgg ataagggaac ggtgcaaaca gaccatgcat gaggcgggga
     5281 aactgctgga ccagtatgag gtcgggcttg cacggcatgt tatagacgat ttcttctgga
     5341 atgatttctg cgacgactac atcgagatcg tcaaggaaag gctttaccag ccggatatac
     5401 atggacatcc ggaaaggaga tcggcccaga gggcattgta tgacagcctg tttgacatcc
     5461 tgaaaatgta tgccatatat gtgccgcata ttacggaata tatctaccag gtatacttca
     5521 ggcagcatga ggatgaaatc tcgctgcacc tgacacggtg ggaagatgcg gaagagttcg
     5581 actatgacat cctggagttc ggcagacagc tgaaagaggc cgtcagccgg gcgagaagga
     5641 tcaaatctgc aagatcgctg tccatgaagg ctgaaataga agggctcgac ataaagacag
     5701 aagagaagta caaggagtat ttcattcggt cgatcccgga tataagggca tgctgcaggg
     5761 ccagggaggt aaaactgtca ataaagtgat tttgtgctga ctgcacaaaa aagctgtcag
     5821 gcatgatatt gatttattgc aaataattgt tgaattaatg tattgcttta tttataattg
     5881 actaaacagc ctgataaggg ttgttacaat ttcacctttc aagatgacag agtaggttat
     5941 tgcgcgttaa gtgccagttg gacgggaagt tgccgctgga cgaaaaatcc ttcttggatt
     6001 tgcggtgcaa tagccgcatt ccgctgtcac atgaggtgag ggcattttcc ttccttctgt
     6061 gacgcagcgt cgttgagaag gagcgctgtc aactgtcgtg tctttgcagt tggcgggctt
     6121 aatgaaagga aggaggtgct tattttatga aaaagacaaa agtcatcgtt tttatgagca
     6181 tacttacagc gctgaatgtt ttgctgaccc atgtggtacc gctcataact actgattttg
     6241 tcaggatcag ttttggcttt atcccgctgt cacttggtgc tatgctcttt ggccctgtaa
     6301 tcggcgggat aggcggtgcg gtaagtgatc tgctgggtat ggtcatcgct ccgaaaggcc
     6361 cttattttcc tggatttacg ctgagtgctt ttctgtcagg cgttatttac ggactgtttc
     6421 tatacaggaa acccaagaac ctgggaagga taacgcttgc agtattgtgc gtttcgatat
     6481 tcgtcaacct gggtcttaat acattatggc ttaccatgct tcaggggaaa ggtttcctgg
     6541 cgctgctccc ggcacgtgcc ttgcagaacg cggtaacctc cgtcgtgcag atagtgttga
     6601 tccccctggt atggaaatat gcgggcagga gtatcgagaa tgaatatctg caccaaccgg
     6661 tttaagcaga gcaggggagt cctggagagg actccctttt catttgtccc gcagtacggc
     6721 atgactattt cattatgtcg aaactgtagc cttgtccgtt gttgtcatcc atattattga
     6781 aagggtcgtg gaagcttagt ttcagaaagt cctctttcag agcaggtatc gtggcttcga
     6841 aacacgtatc ttttttcacc atcgggatat cctgctcatt gtcccagttg ctgccgtaac
     6901 ctaaatggac tgttatttcg gaggctccat tctttgccag cgagccgtcg tagagaatcg
     6961 taattttatc gcctactaca ggcactgctg ggctgatggc cacccctttg tcgagatagt
     7021 caagatttct ggactttttt tcgttcattg tgtcacctct gtcgtttata taccgactgc
     7081 cgtattgcag ccatttttat gaatcatttt tacgatgatt agcatataca tctctgaatg
     7141 aaaaattcgc gggagcatat caatggatta caaaggctgc gctcacagcg gaattttttc
     7201 tggctaattg ccaagactaa atgtgtaaat atccataacg acattgagag ggcacagaaa
     7261 atgaaagcag ttataatggc aggcggtgaa ggaaccaggc tgaggccgct gacatgcaac
     7321 aggccgaaac cgatggtacc ggtcgccgga agacctgtta tggagcacat cgtcagattg
     7381 ctgaaaaccc atggtatcga tgacgtagcc gtaactctgc agtatctgcc ggaccatatc
     7441 atggagtatt tcggagacgg cagcgatttt agcgtgagaa tgagatatta tatcgagaac
     7501 aagcctctcg gcaccgcggg aagtgtaaaa aatgcagaag attttctcga tgagacattc
     7561 attgtgatca gcggtgatgc gcttacagat atcgatctga ctaaagccgt cagcttccac
     7621 agggaaaaag gagccatggc gacgctggtc ctgaaaaggg tggatgtccc gctggaatac
     7681 ggtgtcgtgg ttacggacgg ggacggaaag ataatacgct tccttgaaaa gccgggctgg
     7741 ggtgaagttt tcagtgatac cgtgaataca ggtatatata ttttgtcgcc ggaagtgttc
     7801 gactattacg aaagagatca ggtgtttgat ttcggcaggg atctttttcc gctgctgatg
     7861 aaggaaaaca ggatcatgta cggttatata accgatgact actggtgcga tataggagat
     7921 ataaatgcat atatccaggc caacttcgat gcaatggata aaaaggtaaa ggtaaccgta
     7981 cccggtattg aaatcaggcg tggcgtctgg accggccgtg gtacggtcat cgacgagtcg
     8041 gcctcgatcg aagggccgtg cataattggc gactgctgcc gcataaaagg cggagctgtt
     8101 atagggagcc ataccgtgat cggcgacaac tgcgtcgtgg aagagaacgg cagcatcaag
     8161 agaagcataa tctggaagaa ctcgatcatc ggagaaaaag cagagatcag gggaagtgtg
//
LOCUS       DAOSCC010000106         8178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5705_length_8178_cov_4.335467, whole genome shotgun sequence.
ACCESSION   DAOSCC010000106 DAOSCC010000000
VERSION     DAOSCC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_8178_cov_4.335467"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /gene="thrC"
                     /locus_tag="PLG72_06880"
     CDS             complement(<1..1375)
                     /gene="thrC"
                     /locus_tag="PLG72_06880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ80558.1"
                     /translation="MYYVSTRGWSGRVTSAEAIKRGLAPDGGLFVPDAQVRLDPEEIL
                     SLSKMDYMERAVFILRRFLDDYTDEELAECVSGAYGSGRFDAAGIAPVVKLSENAYML
                     ELWHGPTCAFKDVALQLLPRLIVKANMKTNENCETVILVATSGDTGKAALEGFRDVKG
                     TRIIVFFPDEGVSKVQKMQMVTQEGTNVHVVGVKGNFDDTQNGVKAIFSDEELGRAML
                     GNGYKFSSANSINWGRLVPQIVYYFSAYADMVAAGEIAAGEKINITVPTGNFGNILAS
                     WYAREMGLPVNKLICASNDNNVLTEFINTGIYNREREFKKTISPSMDILISSNLERLL
                     FELTGRDAGQVREWMDKLKNEGRYAIYGEEKRKLNEMFWAEYSTEQETIETIREVYEE
                     YGYLADPHTAVGIDVYEKYVIATGDMTKTVIASTASPFKFNRSVAGAIFGEDEIAGLD
                     EFELLEVL"
     gene            complement(1583..2737)
                     /locus_tag="PLG72_06885"
     CDS             complement(1583..2737)
                     /locus_tag="PLG72_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ80559.1"
                     /translation="MDIKAELNNFRAIDLDEIVRGGEVIPDNVRNSVYLYNKAIEDLR
                     NNSEDMAAIKLKKAVALNPHFNEALNLLGVCYTFIGEKEKAAEAFSKVIRSESNSVYA
                     LSFMQRNKLTDVVPAEEGSGNPVLNLQQQPGEPLKRIRKKKEKHLKPAKPEKPLFDPN
                     RNRRIARNIARFAVGFLSGLLVGAFIYFMLPKPEPVQLPPVEEEIEAAVNEAKRIFDQ
                     QYAALEAKYETAQKEKEDAVKEADYYKAALRLYEIESLVGSKEYESAADMLMLMKTIE
                     FRDAEKDKFDSLYEKVMPLAAKTAYDRGYREYNTKKYEDALKSFEKVRIYDPGYSKMD
                     AALYYMGRCYQLLKDSRNAIALYQEIVDNYPSSWYVKNAKQRINELTKVP"
     gene            complement(2785..4149)
                     /locus_tag="PLG72_06890"
     CDS             complement(2785..4149)
                     /locus_tag="PLG72_06890"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOJ80560.1"
                     /translation="MTYEEALEYINGANRFGKKLGLHNIRTLLRLMGDPQKELRFVHI
                     AGTNGKGSTSAFIGSILSQAGYRTGIYTSPYLQRFNERIAISGPAADADKSCRPDTDA
                     RAADSPGTYRSEISDGELAEITSLVRECADGMVKNGDNQPIMFELVTAVAFEYYRRKK
                     CDIVVLETGMGGRFDATNVIDVPDLAVITTISLDHTQKLGNTLAEIAFEKAGIIKAGG
                     DVLVYDQGDEVMRVFEDACRERGARLHKAAFSTIELHEFGPDGQTFSYKGLDGLKISL
                     IGRHQLRNAAVAVEAAMLLKDKGWSVSEADIRRGLEAARWPGRMEIMSRDPVLIIDGA
                     HNPEGAAVLRKTLDEYFPGRPLTFIVGVAADKDYRTMLETALPGCRKVITVTIPSQRA
                     LPAAELAGHASRYCNDVMTSDTIEGAIKTCMETAGPDDVICAFGSLFYIGAVRDMFCS
                     GRDA"
     gene            complement(4197..6944)
                     /gene="secA"
                     /locus_tag="PLG72_06895"
     CDS             complement(4197..6944)
                     /gene="secA"
                     /locus_tag="PLG72_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ80561.1"
                     /translation="MFKNLVEKIIGTYSERELKRIMPIVDKIEALGPEMEALSDAELR
                     AKTDEFKRRLKDGQTLDDIMPEAFAVVREASTRVLGERHYRVQLIGGIVLHQGRIAEM
                     KTGEGKTLVATLPLYLNALEGKGAHLVTVNDYLAKRDSEWMGKIYNFLGLSVGLIVHG
                     LDNAQRRKAYACDITYGTNNELGFDYLRDNMVIYKEDMVQRELHYAIVDEVDSILIDE
                     ARTPLIISGAGDKSTDLYVKANAFVSRLRAKVFVETDDKQPDDDIDADYIVDEKAHTA
                     TLTERGVAKAEQYFGVENLADPENLTLSHHINQALKAHGLMKRDREYVVKDGEVIIVD
                     EFTGRLMFGRRYSDGLHQAIEAKEGVKVERESKTLATITFQNFFRMYHKLAGMTGTAL
                     TEEEEFQAIYKLDVIEIPTNKPVIRVDHPDCVYKNETGKFRAVINEIEEKHKKGQPVL
                     VGTISIEKSELLSSMLKKKGIPHQVLNAKFHEKEAEIIAQAGRLGAVTIATNMAGRGT
                     DIKLGGNPEFLAKQEMRKQGYPEEVISECTSFAVTDDEVILNARTVYKELYEQYKKHT
                     DAEKEKVVELGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYISLEDDLMRLFG
                     SDRLTSVVEALGLEDDQPIEHRMLTSAIENAQRRVEGKNFDIRKRVLQYDDVMNKQRE
                     VIYSQRRQVLNGESLKDSFMKMIESSVNSLVERFCGEIQHPDMWDWEGINAQVKDLLP
                     DIGKLSVPKEEADTYTKEDLKETLLKSIVGAYEAKEAELGAEVMRELERIVLLRTVDE
                     KWMDHIDAMDQLRYGIGMRAYGQRDPVVEYKFEGFEMFEEMIRSIQYDSVKRLLRMRI
                     NREQGAPKREKVAEPVTASHGDGGPRKPAVRGSDKVGRNDPCPCGSGKKYKKCCGANL
                     S"
     gene            complement(7004..7462)
                     /locus_tag="PLG72_06900"
     CDS             complement(7004..7462)
                     /locus_tag="PLG72_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80562.1"
                     /translation="MIRGLFIGFLILSALMPKSAEIQIVNIMNMANLLPDLPDPGTAC
                     TDKACGLNRPGGCCTAKDPGAEQSNAGEPRAFAAWEIRKAAAAIRNEILPSGSASSAS
                     GQRDIPVVAEPVAIYDSPSLIRYIGQGGNIPVRSGERCFMSGLSPPFEIC"
     gene            complement(7459..7767)
                     /locus_tag="PLG72_06905"
     CDS             complement(7459..7767)
                     /locus_tag="PLG72_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOJ80563.1"
                     /translation="MNSKIRDEWTDGLFEAILLLENLEECYNFFEDICTVAEIKAMAQ
                     RLEVAKMLKQGYTYIDIAKKTGASTATISRVNRCLVYGADGYRTILERLEKKQGGNKA
                     "
     gene            complement(7925..>8178)
                     /locus_tag="PLG72_06910"
     CDS             complement(7925..>8178)
                     /locus_tag="PLG72_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80564.1"
                     /translation="IFISLRVVHGLIMRILTNNRVMLVDVVIPLFFDLFVTFWLLFPQ
                     NNTDIAWRGTRYSVRKGGFIEAVYWEDESGGSGKDRPDA"
BASE COUNT         1674 a         2357 c         1826 g         2321 t
ORIGIN      
        1 acagcacttc gagcaattcg aactcatcca ggccggcgat ctcatcttcg ccgaatatgg
       61 cgcccgcaac gctcctgttg aatttgaacg ggcttgccgt ggacgctatc accgttttcg
      121 tcatatctcc ggtggctatg acgtatttct cgtatacatc gatgccgact gccgtatggg
      181 gatctgccag gtagccgtat tcctcgtata cttccctgat agtctcgatt gtttcctgtt
      241 ccgtggagta ttcggcccaa aacatctcat tcagttttct cttctcttcg ccgtagatag
      301 catatcttcc ctcgtttttg agtttatcca tccattcgcg cacctgtcct gcatcgcgtc
      361 ctgtaagttc aaacaggagc ctttccaggt tgctggatat gagtatgtcc atcgaagggg
      421 atatggtctt tttgaattcc cgttcacggt tgtatatgcc ggtatttatg aattccgtca
      481 ggacattgtt gtcgttggat gcgcatatga gcttgtttac gggaagcccc atctcgcgcg
      541 cgtaccatga agcaaggata ttgccgaaat tgccggtggg cacggtaatg tttatctttt
      601 cgcctgcagc tatctctccg gctgccacca tatcggcgta tgcagagaaa tagtagacga
      661 tctgcgggac aagcctgccc cagtttattg aattggcgga tgagaattta tacccgttcc
      721 cgagcatcgc cctgcccagt tcttcatccg agaaaatcgc cttcacgccg ttctgggtat
      781 cgtcgaagtt gcctttcacg ccaacgacat gcacattggt gccttcctgg gtcaccatct
      841 gcattttctg gaccttgctg acgccttcgt ccgggaagaa aacgatgatc ctggtcccct
      901 ttacgtccct gaaaccttcc agcgccgctt tgcctgtatc cccggaagtc gcgacgagga
      961 tgacggtctc gcagttctca ttcgttttca tattggcttt tacgataagc cgcggcagca
     1021 gctggagcgc cacatccttg aaagcgcatg tcgggccatg ccaaagctcg agcatgtagg
     1081 cgttttcact gagcttaacc accggcgcta tcccggcggc atcaaacctg ccgctcccgt
     1141 aagcgccgct cacgcattcc gccagttcct catccgtgta atcatcaaga aaacgccgta
     1201 atatgaaaac agccctttcc atgtaatcca tctttgaaag agagagtatt tcctcaggat
     1261 ccagtctgac ctgggcatca ggcacaaata acccgccatc aggggcaagt ccgcgcttaa
     1321 tggcttcagc agatgtcact ctcccggacc agccgcgggt gctcacataa tacattcaaa
     1381 aacctccccg gcaaataagt tgcaaagcac cgggcatata tacgcgatgc tttatttaca
     1441 cgatgctata ttataataat acaaatgcgg taaaaataca aaagtaaaaa taaaagcttc
     1501 gtaatggcaa agcttttatc tgtaaaaggc ataattatat ccgcactgcg gcaatgccgc
     1561 tgaaacaggc atcatcagca gatcatggaa ctttggtcag ttcattgatc ctctgcttcg
     1621 cgttttttac ataccagctg gacggatagt tgtccacaat ttcctggtat aatgctatcg
     1681 cgttccggga atccttgagc agctgatagc agcgtcccat gtagtacaaa gccgcgtcca
     1741 tcttgctgta accggggtcg tatatccgta ctttttcaaa gctcttgagc gcgtcctcgt
     1801 acttctttgt attgtattcc ctgtagcccc tgtcgtatgc cgtttttgca gcaagcggca
     1861 tgaccttttc atacaggctg tcgaatttgt ctttctcagc atcccggaac tctatcgtct
     1921 tcatcagcat aagcatgtcg gccgcactct catactcctt tgaaccgacc agagattcta
     1981 tttcgtaaag ccttaaggca gccttgtaat agtcggcctc ttttaccgca tcttcttttt
     2041 ccttctgtgc tgtctcatat ttggcctcaa gtgcggcata ctgctggtcg aatatccttt
     2101 tggcctcgtt aacggcagcc tctatctctt cttcaactgg cggaagctgg acaggttcag
     2161 gtttcggaag catgaaataa atgaaggctc caaccagcaa tcctgacagg aagcccacgg
     2221 caaacctggc gatattccgt gcgatccgcc tgttcctgtt tggatcgaac agtggttttt
     2281 ccggttttgc aggcttcagg tgcttttcct ttttcttcct gatcctttta agcggctcac
     2341 cgggctgttg ctgcaggttc aggacagggt tgcccgatcc ttcctcagcc ggcaccacat
     2401 cggtaagctt gttgcgctgc atgaaactca gcgcataaac gctgtttgat tcggaacgta
     2461 tcactttact gaaagcttcc gccgcttttt ccttttcgcc gataaatgtg taacagacgc
     2521 ccagaagatt aagggcttca ttgaaatgcg gattaagtgc gacagccttt ttgagcttta
     2581 ttgccgccat gtcttcgctg ttgttcctga ggtcttcgat ggctttgttg taaaggtata
     2641 ctgaattgcg gacattgtca ggtattacct cacctccgcg aacgatttcg tccagatcga
     2701 tggccctgaa attgttcagc tcagccttta tatccatcaa aaacctcacc ccctgagtat
     2761 ttggagcaat atgtcgccgg cgtgtcatgc atcccggcca ctgcagaaca tatcccttac
     2821 tgcgcctata taaaacagtg agccaaatgc gcaaatgaca tcatccggcc cggctgtctc
     2881 catacatgtt ttgattgccc cctcaattgt atcacttgtc atcacgtcat tacaatacct
     2941 gcttgcatgt ccagccagtt cagcagccgg aagcgccctt tgagatggga tagttaccgt
     3001 tatgaccttc ctgcatcccg gcagggcagt ctccagcatg gtacggtaat ccttgtcggc
     3061 ggctactcca actatgaacg ttaaaggcct ccccgggaaa tattcgtcca gtgttttcct
     3121 caaaaccgca gcgccttcgg ggttatgcgc gccgtctatg atcagtaccg gatcccttga
     3181 catgatctcc atcctgcccg gccaccgggc tgcttcgagc cctcttctga tgtccgcttc
     3241 cgatacgctc cagcctttat ctttcaaaag catggccgct tcaactgcaa cagcggcatt
     3301 gcggagctga tgccttccga tcagcgatat cttcagccca tcgagccctt tgtagctgaa
     3361 tgtctggccg tcggggccga actcatggag ctcgattgtc gagaacgccg ctttgtggag
     3421 tcttgcgcct ctctccctgc atgcatcctc aaaaacccgc attacttcat cgccctggtc
     3481 atagaccaat acatctccgc ccgcttttat aatgcccgct ttttcgaaag cgatctctgc
     3541 cagcgtgttt cccagctttt gggtgtggtc caggcttatc gtcgtgatga cggcaaggtc
     3601 gggaacatcg atgacattgg tagcgtcgaa ccttcctccc atccctgtct caagcactac
     3661 tatgtcgcat ttctttctgc ggtagtactc gaatgcaact gcagtgacca gttcaaacat
     3721 gatcggctgg ttatcgccgt tttttaccat tccatcggcg cattctctta cgagcgacgt
     3781 gatctccgcc agttccccgt cgctgatctc cgacctgtat gtccccgggc tgtccgccgc
     3841 acgggcatcc gtatccggcc ggcagctttt atcagcgtcc gcggcgggcc cgcttatcgc
     3901 tatcctctca ttgaacctct gcagatacgg cgaggtgtag atgcctgtcc tgtaccctgc
     3961 ttgcgataaa atgctgccaa taaatgctga cgtggaaccc tttccgtttg ttccggcgat
     4021 atggacaaac cgcaattctt tctgcgggtc gcccatcagc ctgagcagcg tcctgatgtt
     4081 gtgaagtccc agtttcttac cgaatctgtt ggctccgttt atatattcca gcgcttcttc
     4141 atatgtcata tgcattacct tgctttataa attctgtcgc tgcatatccc gcaatattat
     4201 gacaggttgg ccccgcagca tttcttgtat tttttgccgc tgccgcaggg gcacgggtcg
     4261 ttcctgccga ccttgtcgct gccgcgcacg gccggtttgc gcggaccgcc gtctccatgg
     4321 cttgcagtga caggctcagc caccttttcc ctcttcggcg caccctgctc acggttgatc
     4381 cgcatgcgca gcagtctctt taccgaatca tactggatgc tcctgatcat ttcctcaaac
     4441 atttcgaatc cttcgaactt atactccacc acaggatccc gctgcccgta cgctctcatg
     4501 ccgatcccgt aacggagctg atccatcgca tcgatatggt ccatccattt ctcgtcgact
     4561 gtcctgagca gcactatcct ttcaagctca cgcattactt ctgcgccaag ttcggcttct
     4621 ttggcttcat atgcgccgac tatggactta agcagcgtct ccttcagatc ttcctttgtg
     4681 taagtgtccg cttcctcctt cggtacactc agcttcccaa tgtccggcag cagatctttg
     4741 acctgggcat tgatcccttc ccagtcccac atatccgggt gctggatctc gccgcagaac
     4801 ctttcgacca gagaattcac ggaactttcg atcatcttca tgaacgaatc tttcaggctt
     4861 tcgccattga gcacctgcct gcgctgggag tatatgacct ccctctgctt gttcattacg
     4921 tcgtcatact gcagcacgcg ttttcttatg tcaaagttct tgccttcgac tcttctctgt
     4981 gcattttcta tcgcactggt aagcatcctg tgctcgatgg gctggtcatc ctcaaggccc
     5041 agcgcctcga ctacggaagt cagcctgtct gagccaaaca gcctcatcag gtcgtcctcg
     5101 agtgaaatat agaaacgcga agatccaggg tcgccctgtc ttccggaacg gccgcgcagc
     5161 tggttgtcga tacgccttga ttcatggcgc tccgtaccga tgatatgaag gcctccaagt
     5221 tccacgacct tttccttttc cgcatcggta tgttttttgt attgctcgta aagctccttg
     5281 taaaccgttc ttgcattcag tatcacttca tcatccgtta cggcgaaact tgtacattcg
     5341 ctgatcactt cctccgggta cccctgcttc ctcatttcct gctttgcaag gaattccggg
     5401 tttcctccga gttttatgtc tgttccgcgg ccggccatgt tggtggctat cgtcaccgct
     5461 ccaagccttc ccgcctgtgc tatgatctcc gcttccttct catggaactt ggcattcagc
     5521 acctggtggg gtataccttt cttcttcagc atgctgctga gcagttccga cttttcgatg
     5581 gagatggtac caaccagtac aggctggcct tttttgtgct tttcctcgat ctcatttata
     5641 acagccctga atttgccggt ttcattcttg tatacgcagt ccggatgatc cactctaatc
     5701 acaggtttgt tcgtagggat ctctatgaca tccagcttgt atatcgcctg gaactcctcc
     5761 tcttccgtga gggcagtacc ggtcatgcct gccagcttat gatacattct gaaaaagttc
     5821 tggaacgtga tggtggcaag cgtcttgctt tccctttcca ccttgacacc ttccttcgcc
     5881 tctatggcct ggtgcaggcc gtcgctgtac cttctgccga acatgagcct gccggtgaac
     5941 tcatcgacga tgatcacttc cccgtccttt acgacatatt ccctgtcacg cttcatgagg
     6001 ccatgggctt tcagcgcctg gttgatatgg tgcgaaagag tcaggttttc cgggtctgca
     6061 agattttcga cgccgaagta ctgttccgcc ttcgctacgc ccctttccgt aagcgttgcc
     6121 gtatgggcct tctcgtcgac gatgtagtca gcgtcgatat cgtcgtcagg ctgcttgtca
     6181 tccgtctcca caaagacttt cgccctgagc cgggatacga aggcgttcgc cttaacatac
     6241 aggtccgtgg atttgtcgcc tgcacctgaa atgatgagcg gcgtccttgc ttcgtctatc
     6301 agtatgctgt ccacctcgtc cactatggca tagtgcagtt ccctctggac catgtcctct
     6361 ttatagatga ccatgttgtc cctaagatag tcgaatccca gctcgttgtt ggtcccatat
     6421 gtgatgtcgc atgcgtaagc cttccttctc tgcgcattgt caagaccatg tacgatcagg
     6481 ccgacactga gccccaggaa gttgtagatt ttgcccatcc attcgctgtc gcgctttgcc
     6541 aggtaatcat tgaccgtgac caggtgggct ccttttcctt cgagggcgtt gagatagaga
     6601 ggcagcgttg ccaccagtgt ttttccttca ccggtcttca tttccgctat cctgccctgg
     6661 tgcagcacga tgccgcctat cagctgaaca cggtaatgcc tctcgccaag aactcttgtg
     6721 gaagcttccc ttaccaccgc gaatgcctca ggcattatat catcaagtgt ctgcccgtcc
     6781 ttcagccgcc ttttgaactc atcagtctta gcgcgcagtt ccgcgtccga tagggcttcc
     6841 atctcaggtc ccagcgcttc tatcttatca acgatgggca ttattctctt tagttccctc
     6901 tcgctgtatg ttccgataat tttttccact aaattcttaa acatattaac ctccgattcg
     6961 tatgaatgtt ctcaattacg ggatagtagc ctgcaattac tggtcagcag atttcaaacg
     7021 gaggtgacag gccggacatg aaacagcgct cgcctgagcg caccggtata tttccgcctt
     7081 gcccgatata acggataaga gacgggctgt catatattgc gacaggctcc gccactacag
     7141 gtatgtctct ttgcccggat gctgacgatg ctgatccgga cggcaggatc tcattcctta
     7201 tggctgccgc agccttcctg atttcccatg cagcaaaggc acgaggctct ccggcattac
     7261 tctgctcggc tccgggatct ttagcggtac agcagccgcc cggcctgttc aggccgcatg
     7321 ctttgtccgt gcacgcagtt ccaggatccg gcagatccgg cagcaaattt gccatattca
     7381 tgatattgac gatctgtatt tccgcactct tcggcatgag tgccgataat atcagaaagc
     7441 ctatgaacag acctctgatc aagccttatt tcctccctgc tttttttcaa gcctctcaag
     7501 tatcgtcctg tagccatcgg cgccatatac gaggcaccgg ttcaccctgc tgatcgtagc
     7561 agtgctggca ccggttttct ttgcgatatc tatataagta taaccttgtt tgagcatttt
     7621 ggctacctca agtctttggg ccattgcctt gatctcggcg acagtgcata tatcttcgaa
     7681 gaaattatag cattcttcca ggttctcaag cagcaaaatc gcctcaaaca ggccgtcggt
     7741 ccattcgtcc cggatctttg aattcatcag gttcatctcc cgcccgtgat acttgcactt
     7801 taatacagta aaatattaaa tcgtgctgta attaacatat caactttttg acccgcagtc
     7861 aatatgctgg aatccgggcg gccgaccgcc catgcatcat ggcggcgata aactgccggc
     7921 gcattcatgc atcgggtctg tccttcccgg atcccccgga ttcgtcttcc cagtaaaccg
     7981 cctctatgaa gccgcctttc ctgacagaat atcttgtccc cctccatgca atatccgtat
     8041 tattttgtgg aaacagcagc cagaacgtga caaacaggtc gaaaaacaac gggatcacaa
     8101 cgtcaacaag catgaccctg ttatttgtca atattctcat aatcaaacca tgcactaccc
     8161 tgagtgatat gaagatga
//
LOCUS       DAOSCC010000107         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5779_length_8092_cov_5.206918, whole genome shotgun sequence.
ACCESSION   DAOSCC010000107 DAOSCC010000000
VERSION     DAOSCC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5779_length_8092_cov_5.206918"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PLG72_06915"
     CDS             complement(<1..1169)
                     /locus_tag="PLG72_06915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOJ80565.1"
                     /translation="MDMKYKDKTLPVEERVNDLLERMTPEEKIGQMMQISFTSVSAEE
                     AEKWVTERCAGSFLHALGEDAARLQEIALNTRLGIPLLFGIDAVRGHALYNGATIFPV
                     QLAMACTWNPELVEKAGRVTAKEVAADGLHWTFSPILCLGRDLRWGRINETFGEDPYL
                     AGVLASSIIQGYQGDSLSDHDSILACAKHYIAYGEATGGRDAYDAQISMRKVREVFLL
                     PFRKAAETGCATFMASYQSIDGTPVTANKKVLRKLLKEELGFDGFVVTDWNNVGSLIF
                     NQHVAGDMETAAKMAIEAGNDMIMSTNEFYEAAVDLVRKGAVPEELIDEAVRRILRIK
                     FRLGLFDGRAYPLPSQAKSVIGCKEHLDTALEITRESIVLLENRNNTLPLSGSVK"
     gene            complement(1260..2258)
                     /locus_tag="PLG72_06920"
     CDS             complement(1260..2258)
                     /locus_tag="PLG72_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ80566.1"
                     /translation="MKTWEGYTRGINLGGWISQCPREQAHYDSFIGEQDIQTIASWGL
                     DHIRLPIDNDVVSDEEGKFTEGIRYVDNCVKWCRDNGLNIILDLHKAPGYAFYNQDNT
                     LFTDAHVQDAFVGLWKEFAARYGKFSDFVSFELLNEVVDAEPGLWNDLVRRAICEIRK
                     VAPDINIVVGGIRWNSASTLELLDPPYDVNIIFTFHFYDPFLFTHQGAKWIKPLSDLS
                     LQYPGNIEDYRRTVKDLNSYDSHYFTVPGMGRDYLVKRMTPAIDIAKKHDVRIYCGEY
                     GVILNAPGESTVSWFKDMNSIFEEYRIGRAAWTYKSMSFGVINWPGDISEQIIRYL"
     gene            2658..5045
                     /locus_tag="PLG72_06925"
     CDS             2658..5045
                     /locus_tag="PLG72_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOJ80567.1"
                     /translation="MGGTFLRKKGKNIALLVVVCMIMMFVTSCGQDTASKDNDGGPGR
                     AAADEGPGAAGEDGGKGGDAGENKAKESSDSSGGAPENEDSQAGAGDSTSGQADSSGS
                     QNAVFATIDFENKTSQGFSGRGGSEVLTVTDEANVTKDGKYSLKVTKRSETWHGPSLR
                     IDNYVQEGAEYKFSVYVKLIEPASAQIMLSTQIGSGSGASYANLAQHNLGVEDGWVEI
                     TGSYRYNTKSDYITIYVESSVPKASFYIDDVSAELAGKAVTGIQTDLVPIKDVYKNDF
                     LIGNAISLQDTYGVRMELLKMHHDIVTFGNEMKPESLQRTKGKFTFSTADRMVENVQK
                     AGLKIHGHTLVWHQQSPQWMNQSSKGVYLGREEALENLRIHIKTVMEHFGDKVISWDV
                     VNEAMNDNPSNPENWRKALRQSPWYHAIGDDYVEQAFLAAREVLDAHPEWDIKLYYND
                     YNLDNQNKSRAVYSMVKELNEKYQKSHPGKLLIDGIGMQGHYSLNTNPVNVEKSLERF
                     TSLGVEVSITELDIMAGTNHELTEKQSIQQGYLYAELFSIFKKYSDKITRVTFWGMDD
                     GTSWRSSMNPTLFDKNLQAKPAYYGVIDPQKFMSENKLEYLESNRSLARYGTPVIDGE
                     TDDIWSKAPALKLNRYQMAWEGATGTARVLWDEKNLYVQIIVNDAQLDKANANPWEQD
                     SVEVFIDENNEKTTFYQEDDGQYRVNFENTATFNPEGIGKGFESAVKVTKSSYMVEMK
                     IPFRTITPKADMQIGFDAQINDAKGGVRQSVANWNDASGNGYQDTSVFGTLTLKK"
     gene            5294..6109
                     /locus_tag="PLG72_06930"
     CDS             5294..6109
                     /locus_tag="PLG72_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOJ80568.1"
                     /translation="MVGILTGCGEKSGGGAGVVKVIDIPLTEEEYAFGVDKNQPELLE
                     EVNKFIAKIKSDGTFDKILNNYFGDGTPSPVTSAELDPSKDQLVVATNAAFEPFEYMA
                     GDKYLGIDMEIAGLLAERLGKELVISNMDFDAVCLSVGQGKADIAMAGLTIKEERKEY
                     VTFSDKYYDAAQMLIVKSSDKRFDNCKTTEDVEAVLNGLDSKTRIGVQNGTTGEFYVR
                     GDEDWGFPGYKAECVGYKSGSLAVQDMLNGNIDLVIIDEAPAKCIAEAFNEMN"
     gene            6232..6942
                     /locus_tag="PLG72_06935"
     CDS             6232..6942
                     /locus_tag="PLG72_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ80569.1"
                     /translation="MGSFEKKVNRFWEIFINQNGYNKVLTGLKNTLVIAVVGLAIGII
                     IGTVIAAIEVMPKYKRLPRILNKICTFYVGLFRGTPIVVQLLLAYYVVLPLLGVNLPP
                     VNVCILVFGLNSGAYVSEIMRGGILSVDPGQMEAGRAVGLSYTVTMMKIVVPQAVKNI
                     LPTLGNELIALVKETSVVSFVGAADLYVAFSYIGSNNYEFMVPYLAMALIYIVLVMII
                     AMLVKIMERSLRKSDRRN"
     gene            6926..7750
                     /locus_tag="PLG72_06940"
     CDS             6926..7750
                     /locus_tag="PLG72_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ80570.1"
                     /translation="MIDVIDLRKSFGGVEVLKGITTTIREGEKVAIVGPSGSGKSTFL
                     RCLNCLEDPTSGSIIFEGVDIADMKVDINIHRRHMGMVFQQFNLFNNKTVLENIMLAP
                     LHVKLAELRKKKLKNVFIRLYNLFATDKKPLYEINTTRQQIRQEVRDNALRLLKRIGL
                     EDKADVYPSTLSGGQKQRIAIIRALAMNPKVILFDEPTSALDPEMVKEVLDLIKQVAE
                     EGMTMVIVTHEMGFAREVATRVLFMDDGQILEENTPDKFFTDPQNPRAREFLSKVL"
BASE COUNT         2171 a         1899 c         2127 g         1895 t
ORIGIN      
        1 tttttcacgg agccggataa cggaagcgtg ttgttcctgt tttcgaggag tactatgctt
       61 tcccttgtta tctcaagcgc tgtgtcgaga tgctctttgc atcctatcac gcttttagcc
      121 tggctgggta atggatatgc cctgccgtcg aacaggccga gcctgaactt gatcctgagt
      181 atcctgcgga ccgcttcatc gatgagttcc tcgggcaccg ctccttttct gacaagatcg
      241 actgccgctt cgtagaattc gttagtgctc attatcatgt catttccggc ttcgatggcc
      301 atttttgccg ccgtctccat atcgcctgcg acatgctggt tgaaaatgag gctgccgaca
      361 ttgttccagt ctgtcacgac gaagccgtcg aatccaagct cttctttcag cagcttcctg
      421 aggacctttt tgtttgcagt caccggtgtg ccgtcgatag actggtaact cgccatgaat
      481 gtcgcgcatc cggtttctgc tgctttcctg aacggaagca ggaagacctc tctgaccttc
      541 ctcatgctta tctgtgcgtc atatgcatcc ctgccgcctg tagcttcgcc atatgcgata
      601 taatgttttg cacaggcaag tatgctgtcg tggtctgaca gcgagtcgcc ctggtatccc
      661 tgtatgatcg aagatgccag tacccctgcc agataaggat cctcgccgaa ggtctcgttt
      721 atccttcccc acctgaggtc ccttccaagg cagagtatag gggagaaggt ccagtgcagc
      781 ccgtcggcgg caacttcctt tgcagtgacc ctgccggctt tttcaacaag ctccgggttc
      841 caggtgcatg ccatggccag ctgcaccggg aagattgtcg cgccgttata cagcgcatgg
      901 ccgcggacgg cgtctatccc gaacagcagc gggattccga gccttgtgtt cagagcgatc
      961 tcctggaggc gtgccgcgtc ttccccgagc gcatgcagga aagagccggc gcacctttcc
     1021 gttacccatt tctcagcttc ctctgcgctt accgatgtga agcttatctg catcatctgt
     1081 cctatttttt cttccggcgt catcctttcg agcaggtcat ttactctttc ctcaacaggg
     1141 agcgttttat ccttgtattt catatccatt gcatcatatc ctcctgtgta ttcaatgtaa
     1201 gacgctgcgc atggcagcat ttcagatata tgccggcgca ttgcggatat atcatttttt
     1261 tacagatatc tgatgatctg ttcggaaata tctccaggcc agttgatcac accgaagctc
     1321 atggatttat aggtccatgc cgccctgccg atcctatatt cttcaaatat tgagttcata
     1381 tctttgaacc agcttactgt gctttcaccg ggcgcattca gaatgacgcc gtattccccg
     1441 caatagatgc gcacatcatg ctttttggct atgtcaatgg ccggcgtcat ccttttgaca
     1501 aggtagtccc tgcccatccc gggaacggtg aaataatgtg aatcatatga attaagatcc
     1561 ttcaccgtcc tgcggtaatc ttcaatgttg cccgggtatt gcaaggacag gtcggaaaga
     1621 ggcttgatcc attttgctcc ctggtgcgtg aacaggaacg ggtcataaaa atggaaggtg
     1681 aaaatgatat tcacatcata cggcgggtcg agcaattcga gggtgctggc gctgttccag
     1741 cggatgccgc cgaccactat gtttatatcc ggagctactt tgcggatctc gcatatggcc
     1801 cttcttacaa ggtcgttcca gagacctggt tccgcatcca ccacctcgtt gagcagttca
     1861 aatgaaacaa agtcgctgaa cttaccgtat cttgccgcga attctttcca taggccaacg
     1921 aaggcgtcct gcacgtgggc gtctgtgaaa agtgtgttgt cctggttgta aaacgcatag
     1981 ccgggtgctt tgtgcaggtc caggataatg ttcaggccat tgtccctgca ccatttgacg
     2041 cagttgtcga cataccttat gccttccgtg aactttcctt cttcgtcaga taccacgtcg
     2101 ttgtcgatcg gaagacggat gtgatcgagc ccccatgagg ctatcgtctg aatgtcctgc
     2161 tcaccgatga agctgtcgta gtgggcctgt tcgcgcggac actgcgagat ccagccgcca
     2221 agattgatgc ccctcgtata cccttcccat gttttcatat acgtttcctc ctccaaagtt
     2281 tgtaaatgat cgttatcggc attaatgctt caatatctgt gttgcctgca gagcagcact
     2341 gcattttcgg taaatcggca acatcggcag cattggtcac attagcaaaa agtgccgtat
     2401 gatttaattg tataccatcg gacaacaatg tcaaacgatt gctagccctg cggtcgggca
     2461 ggtttttaaa acagtgccgc atggatgtat atgccgggcc aaaatggaac ggataacagg
     2521 aattgcctcg ttcctgtttc caattggcaa tttcttttgc cggatgttat acttatacta
     2581 ttggatgtac atctgtcagc gccgatgcca ggtaaaccgc atatggcgtt gaatcaggcc
     2641 agcggaggtg tttggttttg ggaggaacgt ttttaaggaa aaaaggaaag aatatcgcac
     2701 ttcttgttgt tgtctgcatg atcatgatgt tcgtgacatc ctgcggacag gatacggcga
     2761 gcaaagacaa tgacggcggc cctggcaggg cagcggcgga cgagggtccg ggcgctgccg
     2821 gtgaagacgg cggtaaaggc ggggacgccg gtgaaaacaa agcaaaggag tcttcagaca
     2881 gcagtggagg cgcaccagaa aatgaagaca gccaggcagg cgccggcgac agcacatcag
     2941 gtcaggctga cagttccgga agtcagaatg cggtgtttgc aaccatagat tttgaaaaca
     3001 aaacctcaca ggggttttca ggcaggggag gctccgaagt ccttactgtg acagatgaag
     3061 cgaatgtgac aaaggacggg aaatattcgc tgaaagtgac aaaaaggtct gaaacttggc
     3121 atgggccctc gctcaggatc gacaattacg tgcaagaggg cgccgaatat aaattctcag
     3181 tatatgtaaa gcttatcgag ccggcaagtg cgcaaataat gctttctacc cagataggga
     3241 gcggaagcgg cgccagctac gccaatttgg cgcagcacaa ccttggcgtc gaagacggat
     3301 gggtggagat cacaggttct taccgctata acacgaaaag cgattatata acgatatatg
     3361 tcgaaagctc cgttcccaag gcatcatttt acatagacga tgtttccgcg gaactcgctg
     3421 gaaaggccgt gactggtata caaacggacc ttgtccccat aaaggacgtg tataaaaatg
     3481 attttttgat cggcaacgca atatctctcc aggatactta cggcgtcagg atggaacttc
     3541 tgaaaatgca ccacgatata gtcactttcg gcaacgagat gaaaccggaa tcactgcagc
     3601 gcacaaaggg caaattcaca ttcagcactg ccgacaggat ggtcgagaat gtacagaagg
     3661 caggccttaa gatacacggg catacgcttg tatggcatca gcagtcgccc cagtggatga
     3721 accagtcgtc aaagggtgtc tacctgggcc gcgaagaggc gctggagaac ctgaggatcc
     3781 acataaaaac cgtaatggag cactttggag acaaggtcat ttcatgggac gtcgtgaatg
     3841 aagccatgaa cgacaacccg tcaaatcccg aaaattggag gaaagcgctg cgccagtctc
     3901 catggtacca cgccataggg gatgattatg tcgaacaggc cttccttgca gcaagagaag
     3961 tactggatgc ccatcctgaa tgggatataa agctgtacta taacgattac aaccttgaca
     4021 accagaataa atcgcgagct gtatacagca tggtcaagga actgaatgag aaataccaga
     4081 aaagccatcc gggcaagctg ctcatcgacg gtataggcat gcagggacat tattcgctca
     4141 acacgaatcc ggtcaacgtg gaaaaatctc tcgagaggtt tacttcactg ggcgtcgagg
     4201 tgagcataac cgaactggat atcatggcag ggacgaacca tgaactgacc gagaagcagt
     4261 ccatccagca aggatatctg tatgccgaac tattcagcat tttcaaaaag tactccgaca
     4321 agatcacgcg agtgactttc tggggcatgg atgacggtac gagctggaga tcttccatga
     4381 atccgacact gtttgacaag aacctgcagg caaaaccggc ttattacgga gtcatagatc
     4441 cgcagaagtt catgagcgag aacaaactcg agtaccttga atcgaaccgt tcacttgcca
     4501 ggtacggcac tccggtcata gacggggaga cggacgacat ctggagcaag gcgcctgcac
     4561 tgaagctcaa ccgttaccag atggcctggg agggagccac cggaacagca agggtgcttt
     4621 gggacgagaa gaacctttat gtgcagataa tcgtaaatga tgcgcagctc gacaaagcaa
     4681 atgccaatcc gtgggagcag gattcggtgg aagtattcat agacgagaac aatgaaaaaa
     4741 ccacattcta ccaggaggat gacggccagt acagggtgaa cttcgaaaac acggcgacat
     4801 tcaaccctga gggaatcggc aaaggcttcg aatctgcagt caaagttacg aaatcgagtt
     4861 acatggtcga gatgaagata ccgttcagga ccataactcc gaaagccgac atgcagattg
     4921 ggttcgacgc ccagatcaat gacgcgaagg gcggtgtccg ccaaagcgtt gcgaactgga
     4981 acgacgcttc cggcaacgga taccaggata cgtccgtatt cggtactctg acactgaaga
     5041 aatagtccgg aggatgacat aatagtgtat aatcattcaa caatttgtat gattatacac
     5101 tattatattt tacaagccaa attttaaaaa gaattgcata tatattagta ttgacaaata
     5161 aatattctta gtataaattt ataaatatgc agacataaat gcataaaacg tataaatata
     5221 cacttagatt acataaaaag gagagaatgt tatgaaaaaa ctgatagcac ttgtgactgt
     5281 attatcgttg ctcatggtcg gcattctgac aggctgcggc gaaaaaagcg gcggcggggc
     5341 cggtgtggtg aaggtcatcg acataccgct cactgaagag gagtatgcgt tcggcgtaga
     5401 caagaaccag cctgaactcc tggaagaagt gaacaagttc atcgcgaaga tcaagtctga
     5461 cgggacattc gacaagatac tcaacaatta tttcggagat gggactccgt cacctgtcac
     5521 atcagcggaa ctcgatccgt cgaaagacca gcttgtcgtg gctaccaacg ctgcttttga
     5581 gccgttcgag tatatggcgg gggataaata cctcggcata gacatggaga ttgcagggct
     5641 ccttgcagag cgtctcggga aagaacttgt aataagcaac atggactttg atgccgtatg
     5701 cctctcggtc gggcagggca aggcggacat agcgatggcc ggccttacga taaaagaaga
     5761 gagaaaggaa tatgtcacat tctccgataa atattatgat gcggcacaga tgctgatcgt
     5821 caagtcttcc gacaagagat tcgacaactg taagacgaca gaagacgtag aggcagtgtt
     5881 gaacggcctt gacagcaaaa ccaggatagg ggtacagaac ggtactaccg gcgaattcta
     5941 tgtccgcggc gacgaggact gggggttccc cgggtacaaa gctgaatgcg tcgggtacaa
     6001 gtcaggttct ctggcagtac aggatatgct caacggcaat atagaccttg tcatcataga
     6061 tgaagcacct gcaaaatgta tagcagaagc gttcaatgaa atgaactgat accttttgtc
     6121 gatcatacag gtcctgggat aacgggatgc ggtgcctcgc cggccgcggc agggcatcgc
     6181 atcatgtcat ttggtaaaat aaattttcgg tagatattca aggggacggc gatgggtagt
     6241 tttgagaaga aagtaaacag gttctgggag atattcataa accagaacgg ctacaataaa
     6301 gttctgaccg gactgaagaa tacactggtc atagcagttg tcggtcttgc gataggcatc
     6361 attataggaa cggtgatcgc agccatcgaa gtcatgccta aatataaaag gctgccgagg
     6421 atacttaaca agatatgtac attttacgta gggctgttca gaggtacgcc catagtcgtc
     6481 caattgctgt tagcctacta tgtcgtgctg ccgctgctcg gtgtgaactt gcctcctgtc
     6541 aatgtgtgta tcctggtatt cgggctcaac agcggggcgt atgtatccga gataatgagg
     6601 ggcggcatac tttcggtcga cccgggccag atggaagcag ggcgcgcagt cggcctgagt
     6661 tataccgtaa cgatgatgaa gatcgtcgtt ccccaggcag tcaagaacat actcccgaca
     6721 ttgggcaatg aactgatagc gctggtaaaa gaaacttcgg tagtcagctt tgtcggggcg
     6781 gcggaccttt atgtagcatt cagctatatc ggctcgaaca actacgagtt catggttcct
     6841 taccttgcaa tggcgctcat atatatcgtc ctggtcatga ttatcgcaat gcttgtaaaa
     6901 ataatggaaa ggagcctgag aaaaagtgat agacgtaatt gatctcagaa aaagcttcgg
     6961 cggggtcgaa gtgctgaaag ggatcacgac caccatcagg gaaggcgaga aagtcgccat
     7021 cgtgggtcct tccggcagcg gaaaaagtac gttcctgcgc tgcctcaact gcctggaaga
     7081 tcccacttcg ggctccatta tattcgaagg cgtcgacatt gccgacatga aggtcgacat
     7141 aaatatacac aggcggcata tggggatggt tttccagcag ttcaaccttt tcaacaacaa
     7201 aacggtgctg gaaaacataa tgctggcgcc gctgcatgta aaactcgccg aattgcggaa
     7261 aaagaaactg aagaatgtat ttatcaggct gtataactta tttgcaacgg ataaaaagcc
     7321 gctttatgaa ataaatacga cccggcagca gatcaggcag gaagtgaggg acaatgctct
     7381 ccggctgctg aagaggatag ggctcgagga caaagccgac gtgtatccgt caacgctttc
     7441 agggggccag aagcagagga tagccatcat ccgtgcgctg gcaatgaacc cgaaagtcat
     7501 acttttcgat gaacccacat cggcactgga ccctgaaatg gtgaaggaag tactggacct
     7561 gattaagcag gtggcggaag aaggcatgac catggtgatt gtgacccatg agatgggctt
     7621 tgccagggaa gtggccacaa gagtgctgtt tatggatgat ggacagatac tcgaagagaa
     7681 cactcctgac aagtttttca ctgatccgca gaatccaagg gcgagggagt tcctgtcaaa
     7741 ggttctgtga tactggagca tggcagcatt caggccggga acggcaccgg accaggcata
     7801 taacccaggc atataacatg gaatgaaaaa agccttaagg agataaacct taaggccttt
     7861 tgagccggcg aaacatcatc ggctgagtca gtgtaaaaag cgggcaggtc tgtgatcata
     7921 tcagcagcat catcagcgga agcgtcacaa tggaagccag ggtcgtgaaa gcaacgacac
     7981 gggatgcgaa cctggaatcg ccgtcataca tttctgcgaa aatcgtcgtg gtcgctgcca
     8041 ccggcatggc tgtcagaagg acgcagactg tcatcggcat ttgcgggaag cc
//
LOCUS       DAOSCC010000108         8051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_5822_length_8051_cov_4.495748, whole genome shotgun sequence.
ACCESSION   DAOSCC010000108 DAOSCC010000000
VERSION     DAOSCC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5822_length_8051_cov_4.495748"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..918
                     /locus_tag="PLG72_06945"
     CDS             64..918
                     /locus_tag="PLG72_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ80571.1"
                     /translation="MYALPTTQTVVCLFYNKEIFDKYGLKEPQTYDDLKNIIKVLNDN
                     GITPFALGNKAPWVGAMYCELITNRIGGNEPYNKVYNGTGTWEDPAFIEAGKIMQELV
                     NLKAFPEGFNALDNDPAQADFKAGKCGMLVMGSWAIQQLYTDMQDKLAVCKFPEIPGG
                     KGNANHWLGQPDQSFAIAAKSSAKDAAAEFIKTISDPEIQLKLVEAGNLVATTVKADP
                     DKVNPVAMKVSEMLNGMEEFFVFYDVGLGATIGNEYNNTVQAITAGQSVEESFKALQQ
                     FTEQNREQ"
     gene            1145..2032
                     /locus_tag="PLG72_06950"
     CDS             1145..2032
                     /locus_tag="PLG72_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ80572.1"
                     /translation="MDSMVRNKKTIALFLMPALVIYLAIVAVPVLSTVYYSFHKWSLV
                     DARRFVGFSNFIQLFTIDKIFRTSLNNTLLLLVLSIVIQVPLAILLATAISGMTRGKR
                     YFKTVFFMPNILASVAVGLLWSFIYNAEFGLINRLLMAVGLEEYTGLWLADQKTVLGC
                     IMVVICWQFAGYHMIIYLAAIENIPPTLTEMATIDGAGPWQRFRSITLPLIRPIIGID
                     TVLIATGSLRYFDLIYVMSNGGPNHSSEVMALYLYYKSFRDMQYGYGSAIAVVLLCMC
                     LLVTVALNRLFKSEEIEFY"
     gene            2132..2998
                     /locus_tag="PLG72_06955"
     CDS             2132..2998
                     /locus_tag="PLG72_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80573.1"
                     /translation="MGLHAEKSKKKPKLKAGQIRSIVITALLSLVSVTYLYPLLWLII
                     NSLKTDTEMFTDPWSVVPSFQFSNYATAWVSGKIGTSFFNSVIVSLISVGVTIIVASM
                     AAFALKRFKWKLSGFVMGVFLIGVMIPIHSTLIPLFMAFNKIKLLNSYISLILPYVAF
                     ALPTSIFILGGFMATFPREIEEAAVIDGCSMKGVFWRIIFPLSKSSIATISIFNFVNA
                     WNELMFALIFMSEDRKMTLPVSLTRFKGQYSTSWTIQLAAVVIMVLPSLITYFFLNDR
                     IIKSLTIGAVKG"
     gene            complement(3021..5111)
                     /locus_tag="PLG72_06960"
     CDS             complement(3021..5111)
                     /locus_tag="PLG72_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOJ80574.1"
                     /translation="MNSHNGYKAWLAYDQPIWKKPDLKGIFFDSICVEPILQELEIPA
                     RELKEALTAIFGNAPDIVRKPSSGSTILIRLIPHGKAPQTAEPASGPDPAGYISDSEA
                     SRLGSEGFIIKRCSSGDTDLTVIAAKTGTGALYGVFAFIRQILCGRFGEDTYMLQNPG
                     VSLRMLDQWDNMDGSIERGYAGKSIFYKDGQIVRDLKRVEDYARLMASVGLNGIVLNN
                     VNVHWHETRLITREQLPNVARIADVFRKYGIKIFLSINFSSPIELGGLDTADPLDSTV
                     QAWWREKTAEIYEYIPDFGGFLVKADSEFRPGPFTYGRNHAEGANMLAAALKPYGGLV
                     IWRCFVYNCKQDWRDRKTDRANAAYDNFMPLDGCFDDNVILQIKNGPMDFQVREPVSP
                     LIGGLKKTNFMLELQITQEYTGQQRHLCYLAPMWKDVLEFDTYSRGKGSTVARLLSEP
                     ADGMTAGIAAVSNIGDDMNWTGHDLAQANLYCYGRLAWDPQLTAEEIAKEWAMQTFGS
                     DPVVIDTVTGMLLDSYHIYESYTTPLGIGWMINPGHHYGPNVDGYEYSAWGTYHRADH
                     KGLGIDRSVATGTGFAGKYFPPNSTMYEKIETCPEKLLLFFHRIEYTYRLKSGVTLIQ
                     YIYDSHFEGAARAAELIDRWKSIEGRIDPGCFERVLARLREQAEHACEWRDVINSYFY
                     RKSGIPDEKNRPIY"
     gene            complement(5272..6405)
                     /locus_tag="PLG72_06965"
     CDS             complement(5272..6405)
                     /locus_tag="PLG72_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ80575.1"
                     /translation="MSIKDAAMLDVDLEQFWKDDELAHKGNCFSPEAPQVALGIRMSE
                     ECVFAELGEEGNPWGYTPRERRLELNKRYNDKAEQIVGRRLLPETLPEPDAEFPYVKR
                     IGEVFEGKYIHNGVAEWLEGTVSTPAELEKLLDRVEKLNLREFMLPPNWESEKKRIYE
                     KYGIKPPLLRHIRGPVTLATSIYGAENLIFLIIDEPDLAKRFSDVICKVIMGMAEIMD
                     EEAGYDETNRPGGFSFADDNCCLLTPEMYEMFGYPILKAVFDRWSPNPEDMRYQHSDS
                     DMGHLLPILGRLNFTGCNFGPKVLVDEIRKYMPKTRIDGCLSPMTFMSNDIDAIIREV
                     RRDCEMARECRGLNLSTAGSINNGSLLTSMRAVMYAIQKYGRY"
     gene            complement(6521..>8051)
                     /locus_tag="PLG72_06970"
     CDS             complement(6521..>8051)
                     /locus_tag="PLG72_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ80576.1"
                     /translation="PLVNETAKRQMEHPQAAMAIIDVHNGQVKALYGGYGKKEASNTL
                     NRASSSLMKRQPGSTAKPIVVYGPAIDMKLATAATVVDDRAVYMLGEKNPEKPYPTNY
                     NNRYDGLTTIRNAIKNSVNVVAAVVWRDMLGADSSIEYLKKVGINREKEKYISLVLGG
                     WEQGINPLQLAAAYVPFANQGLYYEPTTFTLVKDSNGKVIIDRTKKEASIVYSEETAF
                     IMTELLKEVTKGKTSTYPYSGTAAANINEEIIGMPVAAKTGTTQSNIDKWLVGYTPYY
                     AAAVWYGYDNSGGEPIPVKTEEMYQAHKIWAAVMKKVHEDLPRKEFSEMPEDIVTKQI
                     CIYSGKVATDLCKHDPRGNATITEYFIKGTEPRNDDVCTVHVQAQVCTESKDTMGRNL
                     LAGPYCPADKVETRVFIQRETPYAPKTPNEKAPRDVVYELPAGEYCTVHGAPQTPESG
                     NPLGDLVDWLWPFDRQEGNTPADDAQGGSSRYEDVPGGSTGNVPEENTDYEWPEDVPL
                     E"
BASE COUNT         1771 a         2105 c         2045 g         2130 t
ORIGIN      
        1 tgcccaaaaa gagggacaga cagtgacata aaacctaaaa caagttgaca cgaccacgga
       61 aaagtgtatg ctctgccgac gacacagaca gtcgtatgcc ttttctacaa taaggagata
      121 tttgacaagt atggtctgaa agaaccccag acttacgacg atctcaagaa catcatcaaa
      181 gtcctcaacg ataacggtat tactccattt gcgcttggca acaaagctcc gtgggtcggt
      241 gcaatgtact gtgagctgat cacgaacagg atcggcggca atgaaccata taacaaggtg
      301 tacaacggaa cgggcacctg ggaagacccg gcattcatcg aagcaggaaa gatcatgcag
      361 gaactggtaa acctgaaagc attccccgag ggcttcaacg ctcttgacaa cgatcctgca
      421 caggctgatt tcaaagcggg caaatgcgga atgctggtca tgggaagttg ggctatccag
      481 cagttgtaca cggatatgca ggacaaactg gctgtctgca agttcccgga gatacccggc
      541 gggaaaggga atgcaaacca ctggctgggc cagcctgacc agagctttgc catagctgcg
      601 aaatccagcg ccaaggacgc tgcagctgag ttcatcaaga ccatttctga tcccgagatc
      661 cagttgaagc tcgttgaagc aggcaacctg gtggcaacca ccgtcaaagc ggatccggac
      721 aaggtaaatc ctgttgcaat gaaggtcagc gaaatgctca atggaatgga ggaattcttc
      781 gtattctacg atgtaggtct cggagcgacc ataggaaacg aatacaacaa caccgtacag
      841 gctataacag ccggacagtc ggtggaggaa tccttcaagg cgctgcagca gttcacggag
      901 cagaacaggg aacagtaata cagcgctgtg tatttgaaat ccgcgcggtc ggggcagcct
      961 gaccgccgga tccaataaag ggatggtcca atagcgggcg ctctgaccgc tgcttccgac
     1021 acaagagcgt cttccaggcg ccatgacaca cgagggcatg gcggccggag tgcccggatc
     1081 atttcccgga cctgcttttg ccggtcatga acatcatctt ttgctttgca taaggagtca
     1141 tgaaatggat tcgatggtaa ggaacaaaaa gacgatcgcg cttttcctga tgccggcgct
     1201 tgttatatat ctggcgatcg tagccgtgcc ggtactcagt accgtatatt acagtttcca
     1261 caaatggagc cttgtcgatg ccaggaggtt tgtcggcttc agtaatttca tccagctgtt
     1321 cactatcgac aagatattca gaacaagcct gaacaacacg ctcctgctgc tggtattgtc
     1381 catcgtcata caggtgcctc ttgcgatcct gctggcaacg gcgatatcgg gcatgacaag
     1441 agggaagagg tatttcaaga ccgtcttctt catgccgaac atattggcaa gcgtggctgt
     1501 cggcctgctg tggtcattta tatacaacgc tgaattcggg ctgatcaaca ggctgctcat
     1561 ggctgtcggg ctcgaggagt ataccggcct ctggcttgcc gaccagaaaa ccgtattggg
     1621 ttgtatcatg gtcgtcatat gctggcagtt cgccggctat cacatgatca tctatcttgc
     1681 tgcgatcgag aacataccgc cgaccctgac agagatggcc accatcgacg gagccggccc
     1741 gtggcagagg ttcagaagta taaccctgcc gctgatacgg ccgatcatag gtatcgacac
     1801 ggttttgatc gccaccgggt ccctgcgcta ttttgacctc atatatgtca tgtccaacgg
     1861 cggtccgaac cactccagcg aagtgatggc gctttacctc tactataaat cattcaggga
     1921 catgcaatac ggatatggca gcgctattgc cgtggtcctg ctgtgcatgt gcctgcttgt
     1981 gacggtggcg ctgaacagac tgttcaaatc cgaagagatc gaattctact gaagcacgtt
     2041 agcagctgtt cttctgttga catgcttgtg aagtctgctt acatgctgat gaaatatcaa
     2101 tctttctttg gcaagggaga gtttctgaca aatgggcttg catgcagaga aaagtaaaaa
     2161 gaagcctaaa ctgaaagccg gacagatacg gagcatcgtc attaccgctt tgctttctct
     2221 tgtgtcggtc acgtacctgt atccgctttt atggctcata atcaactcgt tgaagacaga
     2281 tacggagatg ttcaccgatc cgtggtcggt cgttccgtca ttccagttca gcaattacgc
     2341 gaccgcatgg gtgtcaggga aaattggcac gagctttttc aactctgtga ttgtttcgct
     2401 tatatcggtg ggcgtgacca taatcgttgc ctcaatggca gcatttgcac tgaaaagatt
     2461 caaatggaag ctttcagggt ttgtaatggg agttttcctc atcggcgtca tgataccgat
     2521 ccactctacg ctgatcccgc tgttcatggc gttcaataag ataaaactgc tgaacagtta
     2581 catttcgctc atactgccct atgttgcatt tgctttgcct acctcgatct ttatactcgg
     2641 cggctttatg gcaactttcc cgagagagat agaggaggcc gcggtgatcg atggctgcag
     2701 tatgaagggc gtattctgga ggatcatatt cccgctgtct aagtcttcga tagcgaccat
     2761 ctccatattc aatttcgtca acgcatggaa cgagctgatg ttcgcgctga ttttcatgtc
     2821 agaagacaga aagatgacgc tgcccgtcag tctcaccagg ttcaaggggc agtattcgac
     2881 aagctggacg atacagcttg cagcagtagt aataatggtc ctgccaagcc ttatcacata
     2941 cttcttcctg aatgacagga tcataaagag ccttaccata ggagccgtca aaggctgagc
     3001 agaagatcaa cagaccatgt ttagtagatc gggcggtttt tctcgtccgg gatcccgctc
     3061 ttcctgtaga aatacgaatt tatcacatcg cgccattcac aggcgtgttc cgcctgttcc
     3121 ctgagccttg ccaggacacg ctcgaagcag ccgggatcga tccgcccctc gatacttttc
     3181 cacctgtcta tgagctcggc agccctggct gcgccttcga aatgcgagtc atatatgtac
     3241 tggatcagcg ttacgcctga tttgagcctg tacgtgtact cgatgcgatg gaagaacagc
     3301 agcagttttt ccgggcatgt ctctatcttt tcgtacattg tcgaattagg tgggaagtat
     3361 ttgcccgcga agcccgtgcc cgtggcgact gatctgtcga tgccgagtcc cttgtggtcg
     3421 gccctgtgat atgtgcccca tgccgagtat tcatatccgt ccacattcgg gccgtaatga
     3481 tggccgggat tgatcatcca gccgatcccc aggggcgtcg tatagctttc gtatatgtga
     3541 tacgaatcga gcagcatgcc tgtcaccgta tcgatgacga cagggtcgct gccgaaagtc
     3601 tgcattgccc attcctttgc tatttcctcg gctgtcaact gcggatccca cgccagcctg
     3661 ccatagcagt aaagatttgc ctgggcaagg tcatggccgg tccagttcat gtcgtctccg
     3721 atgttggaaa ccgccgctat gcccgctgtc ataccgtctg ccggctccga cagcaatcgt
     3781 gccaccgtgc ttcccttgcc gcggctgtaa gtgtcaaact ccagcacgtc cttccacatc
     3841 ggcgccaggt agcagagatg gcgctgctgt ccagtgtatt cctgggttat ctgcaattcc
     3901 agcatgaaat tcgttttctt cagcccgcct atgagtgggg aaacaggttc cctcacctgg
     3961 aagtccatgg gaccattctt tatctgcagg ataacgttgt cgtcgaagca gccgtccagg
     4021 ggcatgaagt tgtcgtaggc cgcattggcc ctgtcggttt tcctgtcgcg ccagtcctgt
     4081 ttgcagttgt atacgaagca gcgccatatc accaggccgc cgtatggttt gagcgcagcg
     4141 gccagcatgt ttgcgccttc ggcgtggttt ctgccgtatg tgaaggggcc cggacggaat
     4201 tcagagtcgg ccttgacaag aaaaccgcca aagtcaggta tgtattcata tatctcagcc
     4261 gttttttcgc gccaccatgc ctgcacagtg ctgtccagcg gatcggcggt gtcaaggcct
     4321 ccaagttcga tggggcttga aaaattgatg ctgaggaata tctttatacc atacttacgg
     4381 aaaacgtcag cgatccttgc cacgttgggg agttgctccc tggttatcag cctggtttca
     4441 tgccagtgca cgttgacatt gttgagaacg ataccgttca gcccgacgga agccatcagc
     4501 cgtgcatagt cctcgactct tttcaggtcc cggacgattt gtccgtcctt gtaaaaaatc
     4561 gactttccgg catagcctct ttcgatgctg ccgtccatat tgtcccactg gtcgagcatg
     4621 cggagactga cgccaggatt ttgcagcatg taagtatcct cgccgaatct gccgcaaagg
     4681 atctgcctta taaaagcgaa aacgccatag agtgcgccgg ttccggtttt tgccgcgata
     4741 acggtaagat cggtatcacc tgaactgcag cgctttatga taaaaccttc gctgccaagc
     4801 cgggatgctt cactgtcgga aatgtacccg gcaggatccg gtccgcttgc cggttcggcg
     4861 gtttgcggcg cttttccgtg cggaatgaga cggatcagta tcgtgctgcc cgatgaaggc
     4921 ttccggacta tatccggtgc attgccgaat atggctgtca gagcttcttt cagttctctt
     4981 gcggggattt caagttcctg cagtatcggt tccacacata tgctgtcaaa aaatatcccc
     5041 tttaagtctg gctttttcca tattggctga tcgtatgcca gccaggcttt gtagccgttg
     5101 tgactattca tatgtatatc tgacatggtg accctttcct gaaaatttta cttggcggag
     5161 ccataacaga tgaaagatat atcatcatgg accaaatact gcagaggcaa tattatcggc
     5221 gcagcaggca gatttgcctg ccggggttcc taaagcctcc aaaatccggc cctaatatct
     5281 gccgtatttt tgtatagcgt acataactgc gcgcatgctg gtcagaaggc tgccgttgtt
     5341 gatggagccg gccgttgaga gattgagtcc tctgcattcc ctggccatct cgcagtcgcg
     5401 cctgacttcc ctgatgatgg catcaatatc gttgctcatg aatgtcatcg gagacagaca
     5461 gccatctatt cttgttttcg gcatgtattt ccttatctcg tccacgagta ccttcgggcc
     5521 gaagttgcag cctgtgaagt tcagcctgcc cagtattgga agcagatgcc ccatatcgga
     5581 gtcggaatgc tgatagcgca tatcttccgg atttggagac cacctgtcga acacggcctt
     5641 gaggatcggg tatccgaaca tctcatacat ttcgggagtc agcaggcagc aattgtcatc
     5701 agcaaagctg aatccgcccg gacggttcgt ctcgtcatat ccggcttctt cgtccatgat
     5761 ttccgccatc cccattataa ccttgcaaat gacatctgag aacctctttg ccaggtcggg
     5821 ctcgtctatg atgaggaata tgaggttttc agcaccgtag atcgatgtgg cgagcgtgac
     5881 ggggcctctg atgtgtctca gcagcggagg cttgatccca tatttctcat aaatacgctt
     5941 tttttcgctt tcccagtttg gcgggagcat gaattcacgc aggttgagtt tctctaccct
     6001 gtcaagcagc ttttcgagtt cggccggtgt actgaccgtg ccttcgagcc attcggcgac
     6061 tccattgtgt atgtactttc cttcaaaaac ttcgccgatc cttttgacat acgggaactc
     6121 ggcatccggt tccggaaggg tttcggggag cagcctgcgt cctacgatct gttctgcttt
     6181 gtcgttatac cgtttattca gttcaagtct tctttcacgc ggtgtatatc cccatggatt
     6241 gccttcttct ccaagttctg caaagacgca ttcctcgctc atcctgatac ccagggctac
     6301 ctggggggct tccgggctga aacagttgcc cttgtgcgcc aattcatcgt ctttccagaa
     6361 ttgctcaaga tcgacatcca acatggccgc gtcttttatg ctcatagctg tatccctcct
     6421 gccaacataa tacctgttca tccacgacca tacaatggaa ctatggaagt ttctttgtaa
     6481 taattaaggg tagtaataat taagggcaat gttcatggca ttattccagg ggcacatctt
     6541 caggccattc gtaatcggtg ttttcttcgg gtacatttcc ggtgctgccg cccggtacat
     6601 cttcgtaccg tgaacttccg ccctgggcgt catcagccgg cgtgttgcct tcctgcctgt
     6661 caaatggcca cagccagtcg accagatcgc ccaacggatt accggattcc ggcgtctgcg
     6721 gcgcgccgtg gactgtacag tactcgcccg caggcagttc gtaaacgaca tctctcggcg
     6781 ctttctcatt gggcgtctta ggcgcatacg gagtttcacg ctgtatgaac accctcgttt
     6841 cgaccttgtc ggcagggcaa tagggcccag ccagcagatt gcggcccatg gtatccttgc
     6901 tttcagtgca gacctgcgcc tggacatgga ccgtgcagac atcatcatta cgcggctcag
     6961 tgccttttat gaaatattcc gttatcgtag cgttgcccct cggatcgtgt ttacacagat
     7021 ccgtcgccac ttttccggaa tagatgcaga tctgtttcgt aactatgtct tcaggcattt
     7081 cgctgaattc cttccgcgga aggtcttcat gcaccttctt catgacagcg gcccatatct
     7141 tgtgtgcctg gtacatttcc tcggttttta ccggtatcgg ttcgccgccg gagttgtcat
     7201 agccgtacca taccgcagcg gcatagtagg gtgtgtagcc gaccagccac ttgtcgatat
     7261 tgctctgcgt ggtaccggtc ttggcagcca caggcatgcc tattatttct tcattgatat
     7321 tcgcagccgc cgttccggaa tagggatatg ttgacgtctt gcccttggtt acttctttca
     7381 gcagttcggt catgatgaaa gctgtctctt cgctgtatac aatacttgct tcttttttgg
     7441 tcctgtcgat gatgaccttt ccgttgctgt cctttacaag tgtaaatgtc gtaggttcat
     7501 aataaaggcc ctggtttgca aatggcacat atgcagctgc cagctgcagc ggattgatgc
     7561 cctgttccca tccgccaagg accagcgaga tatacttttc tttttccctg ttgatgccga
     7621 ctttcttaag gtattctatg gagctgtccg cacccagcat atctctccac actactgccg
     7681 caacgacatt gacagagttc tttatggcgt tgcgtatggt cgtcagaccg tcatacctgt
     7741 tgttgtaatt ggtcgggtac ggcttttcag ggtttttctc tccaagcatg taaacggctc
     7801 tgtcgtcgac aacggtagca gctgtcgcca gcttcatatc tatggccgga ccgtagacca
     7861 cgatgggctt cgcggtggag ccgggctgcc ttttcatgag cgaggatgaa gccctgttga
     7921 gagtgttgct ggcttctttc ttcccgtagc ctccgtacag agcttttacc tggccgttat
     7981 ggacgtctat tattgccatt gctgcctgcg ggtgttccat ctgtcttttg gccgtttcat
     8041 tcaccagcgg g
//
LOCUS       DAOSCC010000109         7838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6003_length_7838_cov_4.521264, whole genome shotgun sequence.
ACCESSION   DAOSCC010000109 DAOSCC010000000
VERSION     DAOSCC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6003_length_7838_cov_4.521264"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..1201
                     /locus_tag="PLG72_06975"
     CDS             482..1201
                     /locus_tag="PLG72_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ80577.1"
                     /translation="MLSLAILDDNIKVLELYEELIPQWFARNGIKGRIVTATGDYTEF
                     LEAVRSRSVNVCIIDINLQAEISGLYIAQKIRQEKLDVEIIFCTGMLEYMHKAFDVNA
                     YHYIIKPIGKNLERCLVKLNKEMEARQNRKNVLELKTKTNIYYIARNSISHIIRKGNK
                     SIIYSSGSIYEVYDSLDSLIEKLGTDKFIKCCRSEIINRDFIKQIDKIKRKIVLVNGY
                     EGKLSRLKTFRLLKGASDNED"
     gene            1191..2489
                     /locus_tag="PLG72_06980"
     CDS             1191..2489
                     /locus_tag="PLG72_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOJ80578.1"
                     /translation="MRIETLTLLINIFSAFLGSLYIIYFQCTICGLILTRIEKVRFSI
                     TFALLNGVVSSIMHVSIYKPLVLLFLSTIIITVLLKATVIQSFLSFSLYIIGLAVGNS
                     LVPLMISILSPDMAVEEVLADPMLSLGGNLFANFFVFLLFLLIKPIKDYTKMLSQDKF
                     LLVLTAATILVISSTFAMYLYTRTLNMAACLIIASISISYCVFIIMMWISALRRVIKD
                     EDLKQQKFYNESLRSTLFELRRFKHDWINNLTVIDSMLKMNKIDELRQYVSELIGHCW
                     AHTNTQIYSVKNAALFGILSSKINLAYEKGISVNMSVTGELENIPGVKISDFCEIIGI
                     FMDNAIEEAVVTDKVINVDMREGSNFIEMSISNTCTKPLDMQKLATDSYSTKGEGRGM
                     GLAIAKKIISKYKNVLHVTTYEDNIFTQTIEILKTGRKGE"
     gene            complement(2518..2661)
                     /locus_tag="PLG72_06985"
     CDS             complement(2518..2661)
                     /locus_tag="PLG72_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOJ80579.1"
                     /translation="MKKNYFKVLICLLAMAGVFVASTVSAYGCWFWTFYQKECPKALI
                     RED"
     gene            complement(2658..3254)
                     /locus_tag="PLG72_06990"
     CDS             complement(2658..3254)
                     /locus_tag="PLG72_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOJ80580.1"
                     /translation="MFDKISKYVTDMIYSTLDGVDPEKREIIEYGVYMTASEIVKISI
                     ILAVSLVLRIVPYVAAAVAVYGIQRTLLGGIHAKSHLGCMIVHSAIVFGVVAASLYCN
                     IDRAYLLPIIAPFSYLTAYLYAPADLPQKPVKSKKQRRQLRIGGFILLTGLFTASYFL
                     PAVWANIILFSCFLQAVFMTPLAYRVSRNKYGREEVSA"
     gene            3545..3778
                     /locus_tag="PLG72_06995"
     CDS             3545..3778
                     /locus_tag="PLG72_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80581.1"
                     /translation="MLSELAECTVLMLKVIHEMYSTQRITYEEFVTHTRKKLQFLSEN
                     ISQFASEAERENAYDIICKCSSILSGEESSYLQ"
     gene            complement(3802..4887)
                     /locus_tag="PLG72_07000"
     CDS             complement(3802..4887)
                     /locus_tag="PLG72_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ80582.1"
                     /translation="MDEEKVLVMTGGGTSGHVTPNIALLPYLKKEGYSIHYIGSRSGM
                     EKELIMKEGIPYHGISAGKLRRYFDLKNLTDVFRVASGFFQAYSLLGRLRPSALFSKG
                     GFVSCPVVWAAWMRKIPVIIHESDITPGLTNRLSMPFARAVCYTFPETARYIPEGKGI
                     RTGMPIRQSLLEGDRRKGREICGFADAKPVIMVIGGSLGSENINNAVRSILDSLLKDF
                     NVCHICGKGNVKDELNNLNGYKQFEYVNEEQPHLYALADLVVSRAGATVLYELLALKK
                     PNLLIPLSRAASRGDQIQNAASFEKQGYSMVLPEEELDGGSLINAIYDLWRNAERFTV
                     SMKKGTDVSGVQSVLKVIREYSDGRTV"
     gene            complement(4953..5156)
                     /locus_tag="PLG72_07005"
                     /pseudo
     CDS             complement(4953..5156)
                     /locus_tag="PLG72_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5261..5884)
                     /locus_tag="PLG72_07010"
     CDS             complement(5261..5884)
                     /locus_tag="PLG72_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOJ80583.1"
                     /translation="MSRNKGQTYFDAFVELVGYSCKAAGLLKEILNKYNAEQLPGKMK
                     EMHEIEHGGDQARHRMIQKLAREFITPIDREDIMALADSIDTVTDTIEDVLMRMYMFN
                     IGKVTEHALKMTEVIVKCCNALKTALDEFYNFRKSAKLHDLVVEVNRLEEDGDALYME
                     ATRKLYVECDNYKELAAWDTIYHYLEKCCDSCEDVADAIESVIMKNS"
     gene            complement(5919..6971)
                     /locus_tag="PLG72_07015"
     CDS             complement(5919..6971)
                     /locus_tag="PLG72_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOJ80584.1"
                     /translation="MTVTLTDFLNKLVTTPALMVTVILTLGVILVNGWTDAPNAIATC
                     ISTRSMSPRPAIVMAAVFNFLGVLVFSLVNAKVAETIYNMVDFRGDSHEALVALCAAM
                     VAIVLWATAAWWFGIPTSESHALIAGISGAALALQRGIGGINPAEWVKVIYGLVLSVA
                     MGFVGGVLVVRLVELIFRGFNRRKTYSFFKYAQIAAAAGMAFMHGAQDGQKFMGVFML
                     GVFLAQGQAGVTIFTIPLWLIFLCSAIMALGTSIGGMRIIKSVGMDMVKLETYQGFSA
                     DMMGTVSLLTANFLGLPVSTTHTKTTAIMGVGAAKRITSVNWGVVKEMMMAWILTFPG
                     CGLIGFLMAALFMRIF"
     gene            complement(7252..7326)
                     /locus_tag="PLG72_07020"
     tRNA            complement(7252..7326)
                     /locus_tag="PLG72_07020"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7292..7294),aa:Ala,seq:cgc)
     gene            7572..>7838
                     /locus_tag="PLG72_07025"
     CDS             7572..>7838
                     /locus_tag="PLG72_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ80585.1"
                     /translation="MKLTVKECITVALFTALMVAGAYLRIPFPLLPVTLQTFICALSG
                     LVLGARLGALSMTVYMCLGLAGLPIFASGGGPASAADASRAPLCR"
BASE COUNT         2129 a         1956 c         1627 g         2126 t
ORIGIN      
        1 gcctatatcc atggcaaaca cgatgtcatc cggagcaata tctttaacag cggctggtgt
       61 ttccgggttt ttgccctcca gcctgatcac tcttccgccg cgtttcttat ttgcactgtc
      121 tgtggctctc atgccgcttt ccttgtccgg actggtgcca tgatacttgc ctgccacagc
      181 tatatcacct ctcacacatg catatggcgg acccatttcg cctgatccgc ctttataggc
      241 tcagctgcat tggtttgata actaatatta attcgacagt taaaagttaa atccttctgt
      301 cgagttgccg atttatttat cgtacgctgc cgcataaaag caacaggtcc ggactcctgc
      361 catggatggg actggagtcc ataggtccaa tttgtacttt tcaacgcctg ttcatgatag
      421 tatactgttt atgtaaatag ataacaaacg tataatgctt cagcaggaaa ggagaacaaa
      481 agtgctgtcc cttgccatcc tcgatgacaa cataaaagtc cttgaactgt atgaagagct
      541 gattccccaa tggtttgcca ggaatgggat caaaggccgc atcgttacgg ccaccggcga
      601 ctacacagag ttccttgaag cagtaaggtc acgctccgta aatgtatgta tcatcgacat
      661 caatctgcag gctgagataa gcggccttta catcgcccag aaaataaggc aggaaaaact
      721 tgatgtcgag atcattttct gcacgggcat gctggaatac atgcataagg catttgacgt
      781 taacgcttac cattatataa ttaaacctat aggcaaaaat cttgaaagat gtctcgtaaa
      841 gctgaacaaa gagatggaag ccaggcagaa caggaaaaat gtactcgaac tgaaaacaaa
      901 aacgaacata tattacattg cccgcaactc aatatcgcat atcataagga aaggcaataa
      961 atccataatc tactcttccg ggagtatata cgaagtgtat gacagtctcg attcactcat
     1021 tgaaaagctt ggcacggaca aattcatcaa atgctgcaga tccgagataa tcaacaggga
     1081 ctttataaaa caaatagaca aaattaaaag aaaaattgtc ctggtaaacg gatacgaagg
     1141 aaagctcagc cgtctcaaaa cattccgtct gttgaaagga gcttctgaca atgaggattg
     1201 aaactttaac tttactgata aatatttttt ctgcatttct tggaagtctc tatatcatct
     1261 attttcaatg caccatatgc ggacttatac tgacacggat cgaaaaagtc aggttttcga
     1321 tcacatttgc gctgctgaac ggcgtcgttt catccataat gcatgtatcc atatacaaac
     1381 cgttagtgct tctgttcctc agcacgatta ttatcacggt tttgttaaaa gccacggtta
     1441 tacagtcgtt cctgtcattc tcactttaca ttatcggact ggcagtggga aactcgctcg
     1501 tgccgctcat gataagcata ttatccccag acatggctgt tgaagaagta ctggccgatc
     1561 ccatgctgtc tcttggagga aatctttttg ccaacttctt cgtatttctg ctgttccttc
     1621 tgatcaaacc cataaaggac tacacaaaaa tgctgagcca ggacaagttc ctgcttgtac
     1681 tcactgctgc tacgatcttg gtcatatctt cgacatttgc aatgtacctg tacaccagga
     1741 cccttaatat ggcagcctgt ttgataatcg catcaataag catttcctac tgcgttttca
     1801 taataatgat gtggatctcg gcgctcagaa gagtgatcaa ggatgaagac ctgaaacagc
     1861 agaaatttta caacgaatcg ctccgatcca ccctgttcga actcagaagg ttcaaacatg
     1921 actggatcaa taatctgact gttatcgact caatgctcaa gatgaacaaa atcgacgaac
     1981 tcaggcagta cgtctcggaa cttatcggac attgctgggc tcacacgaac acgcagatct
     2041 attcagtcaa aaacgccgcg ctgtttggca tcctctcatc aaaaatcaac cttgcctatg
     2101 aaaaaggcat atcggtcaac atgtctgtta ccggtgagct tgaaaacata cctggagtaa
     2161 agatatctga tttctgcgaa ataatcggga tcttcatgga caatgccatc gaagaagccg
     2221 ttgtcactga caaggtgata aatgtagata tgagagaagg aagcaacttc atcgagatgt
     2281 ccatctcaaa cacctgcacg aaacctttgg acatgcagaa actggcaact gacagttatt
     2341 caacaaaagg cgaaggcagg ggcatgggac ttgcaatagc gaaaaaaatc atttccaagt
     2401 ataaaaatgt cctccatgtt accacatatg aagacaatat attcactcaa acgatcgaaa
     2461 tactgaaaac aggcagaaag ggggagtaat cccccctttt gcccggcatt attccattta
     2521 gtcttccctg ataagtgcct tcgggcattc cttctggtag aatgtccaga accagcatcc
     2581 gtatgcggaa acagtggatg caacgaacac gcctgccatt gcgagcaagc atataaggac
     2641 cttgaaatag ttcttcttca tgcagacacc tcctctcttc catatttgtt tctcgatacc
     2701 ctgtatgcca aaggcgtcat aaacacagcc tgaaggaaac acgaaaagag tatgatattc
     2761 gcccatacgg caggaagaaa gtacgaagcc gtaaacagac ctgtcagcaa tatgaagcct
     2821 ccgatcctaa gctgtctcct ctgttttttc gatttgacag gcttttgcgg aagatccgcc
     2881 ggcgcataca gatatgccgt cagatatgaa aatggagcta ttatcggcaa taagtacgca
     2941 cgatcgatat tgcaataaag cgaagccgca acgacgccaa aaacaatcgc ggaatgtacg
     3001 atcatacatc caagatgcga tttagcatgt atccctccca gcagtgtccg ctgtattcca
     3061 tatacggcca cggcggcggc aacatacgga actatcctga ggacaagcga aacagccagg
     3121 atgatgctta tcttgactat ctctgaggca gtcatataaa cgccgtattc tatgatttca
     3181 cgcttttcag ggtcaactcc gtcaagagtg gaatagatca tatctgtcac gtatttgctt
     3241 attttatcga acatcggcat cacctgttaa tattatacat gataatcata tgcttcctga
     3301 agtatttaat cgaataatac tttttctttg gcgaaaacat atttttacgt ttcagtacga
     3361 taaactactc ttttttgcca tatatacagt atttttttaa cgaatcttac gaacttttta
     3421 tatagtggcg ggcaataccg atggaaataa tcatatatat tgacaaataa tacttatgat
     3481 gatatcttat tttcgacgaa tcatagataa tcgcagaaat gcaataatgt gaagaggtta
     3541 tggtatgtta tccgaattag ctgaatgcac tgtgctcatg ttgaaagtga tacatgagat
     3601 gtacagcaca caaaggataa cttatgaaga atttgtaacc cacaccagga aaaagcttca
     3661 gtttttatca gaaaacattt cacagttcgc gtctgaagca gaaagagaaa acgcatatga
     3721 cattatctgt aagtgcagct cgatactgtc gggagaagaa agcagctacc tgcagtaaaa
     3781 cagctaaatg ccgcctgcgt tttataccgt tcgcccgtcc gaatattctc ttataacctt
     3841 taacacagat tgcactccgc tgacatcggt gccttttttc attgacaccg tgaaacgttc
     3901 cgcgtttctc caaagatcat atatggcatt tatcagggat ccgccatcca gttcctcttc
     3961 gggcaaaacc atgctgtatc cctgcttttc aaaggaagcg gcattttgaa tctggtctcc
     4021 gcggctggcg gctctcgaca gcgggatcag cagattcggc tttttcaggg caagcagttc
     4081 atacagcacc gtagcgcctg cccgcgatac gaccaggtct gccagtgcat aaaggtgagg
     4141 ctgctcttca tttacatact caaattgctt ataaccgttt aagttattca attcgtcctt
     4201 tacgttgccc ttcccgcata tatggcaaac gttgaaatcc ttcagcagac tgtccagtat
     4261 gcttcttacc gcattgttga tattctctga cccaaggctg cctcctatga ccatgatgac
     4321 aggcttggca tcagcgaatc cgcatatttc cctgcctttc cgtctgtcgc cttccagcag
     4381 gctctgcctt atcggcatgc ccgtccttat gccctttcct tccggtatgt accgtgcggt
     4441 ttcagggaaa gtatagcata cagccctggc aaacggcatc gaaagcctgt tggtaagccc
     4501 gggcgttatg tcggactcat gtatgatgac agggatcttt ctcatccagg cagcccagac
     4561 cacagggcag gatacaaagc cccccttgct gaacagcgct gaaggcctca gcctgccgag
     4621 cagcgaatat gcctggaaga accctgaagc taccctgaaa acatcagtga gattcttaag
     4681 atcgaaatac ctgcgcagct tgcctgcact gatcccatgg taaggtatcc cttccttcat
     4741 gatcagttcc ttttccattc cgctgcgcga gcctatgtaa tgtatgctgt agccttcctt
     4801 tttcagatag ggcagcagag cgatattggg agtcacatgg ccggatgttc ccccaccggt
     4861 catgacaagt accttctctt catccatatc aatcaactac ctcgttcctg cgcaaaacgc
     4921 catatcaatt cctgacggat cctggaacgc cctcagccca aaatagggct gcactgcgat
     4981 ctctctctat tatatatatc tatttctttc tgcctattcg ccatgtattc cttcaaatat
     5041 tctatccttg acagcctttc gatcatttca tcggtcagaa acccgatgct ggtcacatct
     5101 atattgacgc tgcgcttgat cctgcgtccg cccgaggttt tcatgcccct ccggttcttg
     5161 aaggaatcgg aaacctatgc ccaggccgct gaaacactca tctatccagg cctgcaacat
     5221 gatcccccga cgtatatgat acctgagccg aatcaaattg tcaggagttc ttcattatga
     5281 cgctctctat cgcatcggcg acatcctcgc atgaatcgca gcatttttca agataatggt
     5341 atatcgtgtc ccatgcagcc agttccttgt agttgtcgca ttctacgtaa agcttccttg
     5401 tcgcctccat gtacagggcg tctccatctt cctccagcct gtttacctcc actaccaggt
     5461 cgtgcagctt cgccgattta cggaaattgt agaactcgtc gagagctgtt tttaatgcgt
     5521 tgcagcattt tactatgact tctgtcattt tcagtgcatg ctccgttacc tttccgatat
     5581 tgaacatgta catccgcatg agcacgtctt ctatcgtatc tgtaaccgta tcgatagagt
     5641 cggccagagc catgatgtcc tcgcggtcta ttggtgtaat gaattccctg gccagtttct
     5701 ggatcatcct gtgcctggcc tggtccccgc cgtgctctat ttcgtgcatc tccttcattt
     5761 tcccgggcag ttgctctgca ttgtatttat tcaatatctc cttgagcagg ccggctgcct
     5821 tgcaggaata gcctaccagt tcgacaaatg catcaaaata ggtttgtcct ttattccttg
     5881 acatattccg atcatcctca taaaccttat tttttacttc agaatatcct cataaacagt
     5941 gcagccatca aaaaacctat caggccgcag ccagggaaag tcagtatcca tgccatcatc
     6001 atctctttta ctactcccca gttgaccgaa gttatccttt tagccgcacc cacacccatg
     6061 atagccgtcg tcttcgtatg cgtggtgctg acaggaagtc cgaggaaatt cgcggtcaat
     6121 aggctcacag tccccatcat gtctgcggaa aagccctggt atgtttccag cttgaccata
     6181 tccatcccga ccgattttat tatcctcatg cctccgatcg aagtgccgag cgccattatt
     6241 gcagagcaca ggaatatcag ccacaggggg atggtgaaaa ttgttacgcc ggcctgtccc
     6301 tgtgcaagga acacgccgag catgaatacg cccatgaact tctgaccgtc ctgcgccccg
     6361 tgcatgaatg ccatacccgc agcagcagct atctgggcat atttgaaaaa agaatatgtc
     6421 ttcctcctgt tgaaccccct gaatatgagt tcaacaagcc tgaccaccag cactcctccg
     6481 acgaatccca ttgcgacaga gagtacgagc ccgtagatca ccttgaccca ttcagcggga
     6541 ttgatgccgc ctatcccgcg ctgaagcgct aaggcggccc cggaaattcc tgcgatcagc
     6601 gcatggcttt cgctggtggg gatgccgaac caccatgcgg ccgtcgccca caggacgatg
     6661 gcgaccatgg cggcgcacag cgctacgagc gcttcatggc tgtctcccct gaaatcgacc
     6721 atattataaa tagtttcggc aacttttgcg ttcacaagtg aaaaaacaag tacaccgaga
     6781 aagttgaaga cagcagccat tacaatggct ggtctcggac tcatcgaacg tgtggatatg
     6841 catgtggcga tcgcattggg ggcgtctgtc catccgttta ccagtatgac gcccaatgta
     6901 agaattacgg tcaccatcaa tgccggagtc gtaacaagtt tatttaggaa atccgtcaat
     6961 gtaacagtca tcgaccatca actttcctga ttaagattat gtatatttaa tatgtacatt
     7021 atcaagcgtt tttagcatac catgtttttt ttatccggac aatatcagaa agcgtaaatc
     7081 attccggaaa aagctttttt tgacgaaaca ggaaaaatag ccgcaatttt cgacgaaaat
     7141 ttggttgttt taacgaagga tgaatattac ggcctcttta ctctccagga tgcggctttc
     7201 aacagcactt aaactgaaaa cctgcttaat agcaggtttt catacggttc atggtggagc
     7261 tgatgggatt tgaacccacg actttcacaa tgcgaatgtg acactctccc tctgagttac
     7321 agcccctcat atcatatttg cagttcatat aacaatatac acctttactg aaaccaggtc
     7381 aacagcttgc tgcaaaaact aaatgtcaac ctttattttt tatatggttg acaatcggct
     7441 caaaagtata tattctaagt ataaagatca aaagacccgg cgtatgtcaa cggtatcctg
     7501 caagaaggga acctgcggat tgtccgggct caccgaaacc agggctgatt ttgctgaaag
     7561 aaggggttat tatgaaactg actgtaaaag aatgtataac tgttgcatta ttcacagctt
     7621 taatggtcgc cggagcgtac cttcgcattc cattccctct gctgcccgta accctgcaga
     7681 cttttatatg cgccctttcc gggctggtcc tcggcgcaag gctgggggca ttgtccatga
     7741 cagtctacat gtgccttgga ctggccggcc ttcctatatt cgcaagcggc ggcgggcccg
     7801 catcggccgc agacgcatcc cgggcaccgt tgtgtcgc
//
LOCUS       DAOSCC010000110         7739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6094_length_7739_cov_4.317283, whole genome shotgun sequence.
ACCESSION   DAOSCC010000110 DAOSCC010000000
VERSION     DAOSCC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_7739_cov_4.317283"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLG72_07030"
     CDS             <1..727
                     /locus_tag="PLG72_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ80586.1"
                     /translation="FSLEKGETLSIIGSSGSGKTTLLRCLNFLETPDAGTITVNGEVI
                     FDAADPVSRSEKGIREKRLHFGLVFQSFNLFPQYTALQNVTLAGLLLAKERADYKGRK
                     NEIQEEIIRNAKTILDSVGLSSKLDYYPHQLSGGQQQRVAIARALALNPDILCFDEPT
                     SALDPELTGEVLRVIRELAERKTTMIIVTHEMSFARQVSDNVLFMDDGVIVEYGPPEQ
                     VIGNPREERTKRFLERYFENGNV"
     gene            complement(724..1974)
                     /locus_tag="PLG72_07035"
     CDS             complement(724..1974)
                     /locus_tag="PLG72_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ80587.1"
                     /translation="MDSMELAELWRRYDLFKKRGLKLDMSRGKPEKAQLDLSVPMLRM
                     DYEFTNDDFISEDGIDVRNYGDPTGIPEARRLFSQLLGVPASQVIVGGNSSINMIHDV
                     LTRALLCGPMEGDTPWAKLPRVRFICPVPGYDWHFHMCETLGIGMVPVELGPGGPDMD
                     KVEELVKDPEVKGLICVPMYSNPSGITFSDETVIRLASMKTAAPDFRIIWDNAYCVHH
                     LYEHERDSLMNIYEACAAAGIPDRVLMFTSTSKITFAGGGVAAMGASPANIARHSALL
                     TYQLVCYDKLNQLRHVRFLPDKAAVEAHMAKHAGIIRPKFEYILDVLENELSGIGTWS
                     RPKGGYFICYYAPEGCARRIVELCRDAGVTLTPAGSSYPYGIDPKDSTIRIAPTYPPL
                     SELKLVMELFPVAVKIAVAEKYRH"
     gene            complement(2128..3495)
                     /gene="larA"
                     /locus_tag="PLG72_07040"
     CDS             complement(2128..3495)
                     /gene="larA"
                     /locus_tag="PLG72_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOJ80588.1"
                     /translation="MALRLPYNRTEVCCSAYIRIEKEIEMPYYLKYGNREFEVRLPGE
                     QIKLVLEPNHVELPSRTPRELIEEALDNPIGAKRIEETVKLGEKISIVISDVTRSWQK
                     PGMILSVLLERLNRAGIRDEDIVIISARGTHRAQTAGELRALVTDEIYSRIRVVDHDP
                     SDESNLTYLGTTSRGTPVKLNSIAVNSDHVILIGAVLHHFLAGFSGGRKSVIPGIASR
                     ETVQANHSLSLNHGLGSGGNPDVKSGRMEGNPLHLDMLEGALMLGRCFIINVVADDSY
                     RIVGAFAGDMVKAHEEACRLVDRMNKVRIDRLYPIVIASAGGYPKDINVYQPLKTLCH
                     MLECTEPGGIMIMLSESREGFGSPDTEKQITGYDNMLDREKALREKYSIGAHTGYLFA
                     DTAEKYTFIYVTDFGAENFSKTKMHVVRTLDEALDLAAALNGGKLTGDVLLMPNGSVT
                     LPELV"
     gene            3942..4583
                     /locus_tag="PLG72_07045"
     CDS             3942..4583
                     /locus_tag="PLG72_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ80589.1"
                     /translation="MYELIIFDIDGTMIDTAEVVKKSYSYGMYEELGRDLTDEEVARA
                     FGVPTIQAMEILGARNIEKACGRYFESLFKYYREGVPLFEGIEELLQELKRRGVKCGI
                     VTSRNRDEVANDTTLSSVMKYFSLIVTAEDTKKHKPEAEPVLKLMELAGVVPAGVIYI
                     GDTYFDYMCAKNAGIDFALASWGAREDDRIRPDYLLARPADLLDILDRGREQD"
     gene            4756..6279
                     /locus_tag="PLG72_07050"
     CDS             4756..6279
                     /locus_tag="PLG72_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ80590.1"
                     /translation="MGAYVIGIDIGTQGTKAALFDEKMETIATAFAPSRLISPKPGTV
                     WQEPDDLYMSCAATIKELLDKSGVAAKDVAAIGVDGQMAGIMGVDENGEASTYYDSWL
                     DTRCGKYMEQMRSRAGKRIIELSGGPVSYVHGPRILWWKNEHPEAYAKTARFVMPQAY
                     VVSRMTGLTADETYFDYTHLHFSCLADNANKCWSDELLEIFDIDKNKMARIISPFEVV
                     GRVTEDFAAISGLVEGIPVAAGCGDTAASTFGAGMFDRGQVLDCAGTASVLCSVVDSF
                     VPDTGHETLTMMRSPIDGLFLPLAYINGGGMCVRWFRDTLSGKPAAGYDELEKEAESV
                     EPGSEGLLFIPHFSGRALPCSTSLKGAWMGLDFKHTRGYMYRSVMESIAYEYRYYLSV
                     LRSLYPKGDFSSMVTIGGGAKSALFNQIKADVLGIGVTVFETGDTALLGSAVIAAYAV
                     GMLTDYKQPIRRVMKKRAEYVSNAEKHDAYRFYADAYLRAIDRLTPFYDELASGEIG"
     gene            6362..7165
                     /locus_tag="PLG72_07055"
     CDS             6362..7165
                     /locus_tag="PLG72_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ80591.1"
                     /translation="MAKQSGAMLAQVRQQTIAELVQKMGNITVNDLCARFNVSPATIR
                     NDLRSLESRSIIKRTHGGAISTSKMVYELSTVQKEVRSINEKRAIARTAAEFIEEGDA
                     IALDSGTTTFELARLLVNIKNLTVVTNDLQIASWLEQNTSVNIMLAGGQVRRNFRCTT
                     GQSALEMLSMLHVDKAFIAVNGVSIKSGLTTPTMDMACIKKQMIDSADKVILLADSSK
                     IGRTAFVTYAPVSVLDVMITDEFADNSFVVSLRSTGVNVICAKVDDAGR"
BASE COUNT         1923 a         1891 c         2141 g         1784 t
ORIGIN      
        1 cttcagcctc gaaaaggggg agaccctttc catcatcgga tcctccggaa gcggaaagac
       61 aacgctgctt cgctgcctca acttccttga gactcccgat gcaggcacta tcaccgtaaa
      121 cggggaagtc atattcgacg ctgccgatcc cgtttccagg agcgagaaag gcatacgtga
      181 gaagcgtctg cacttcgggc tcgttttcca gtcgttcaat ctcttcccgc agtatacggc
      241 cctgcaaaac gttaccctgg caggattgct gctggcgaaa gaacgtgctg attacaaggg
      301 gagaaagaac gagatacagg aagaaataat caggaatgcg aaaaccattc tcgacagtgt
      361 cgggctttcg tcgaaactcg attattatcc gcaccagctt tcaggcggcc agcagcagcg
      421 cgttgccata gcccgcgcgc ttgcgctgaa cccggatata ctttgcttcg atgaacccac
      481 gtccgcactc gacccggagc ttaccggcga agtattgcgt gtgatacgcg aacttgccga
      541 aaggaagacc acgatgataa tcgtgacgca cgaaatgtcc tttgcccggc aggtgtccga
      601 caatgtgttg ttcatggacg atggagtcat tgtcgaatac ggcccgccgg agcaggtcat
      661 aggtaatccc agggaagagc gcacaaagcg gttccttgag cgttatttcg agaacgggaa
      721 cgtttagtgc ctgtattttt cggcgactgc tatttttacc gccacgggga agagctccat
      781 gaccagtttg agttccgaca acggcggata tgtgggtgcg atgcgtatcg tgctgtcttt
      841 cgggtcgatg ccgtagggat atgacgaacc tgccggggtc agggtgacgc cggcgtcgcg
      901 gcacagttcc acgatccgcc tcgcgcagcc ttcaggcgcg taatagcaga tgaaataccc
      961 acccttcggg cggctccagg tacctatgcc cgagagttca ttttcgagca catccagtat
     1021 atattcaaac ttcggacgga ttattccggc atgctttgcc atgtgtgcct ccactgcagc
     1081 cttatcaggc aggaagcgga cgtggcggag ctgattcagc ttgtcgtagc ataccagctg
     1141 atatgtgagc agggcgctgt gtcgggctat gtttgccgga cttgcaccca ttgcggcaac
     1201 gccgcctccc gcaaaagtta tctttgaagt cgaagtgaac atgagcaccc tgtccggaat
     1261 tccggcggca gcacatgctt catatatgtt cataagactg tcgcgttcgt gttcgtagag
     1321 atggtgtaca cagtaggcgt tgtcccatat gatcctgaag tccggcgcgg ccgttttcat
     1381 ggatgcgagc cgtatcacag tttcgtcgct gaatgttatg ccggacggat tgctgtacat
     1441 gggaacgcaa atcagcccct tgacctccgg atcctttaca agttcctcca ctttgtccat
     1501 atccggtccg ccgggaccga gttccacagg taccatccct atgccgagcg tttcacacat
     1561 atggaaatgc cagtcatacc cggggacggg gcaaatgaac ctgaccctgg ggagcttcgc
     1621 ccatggtgtg tcgccttcca tcggcccgca cagcagtgcg cgcgtaagca cgtcatggat
     1681 catgtttatg ctggaattgc cgccgacgat gacctggctg gcaggaacgc cgagcaattg
     1741 ggaaaaaagg cgcctggcct ccggtatccc tgtgggatcc ccataattac ggacatcgat
     1801 gccgtcttcg ctgatgaaat catcgttggt aaattcataa tccatgcgga gcatcggtac
     1861 agagaggtca agctgtgctt tttcgggttt cccccgtgac atgtccagtt taaggccgcg
     1921 ctttttgaac aggtcatagc gccgccaaag ttcggccagt tccattgagt ccataatctc
     1981 cactctttcg taagaacatt caggtatacg ttttacgtat cctgttatac agatccggta
     2041 tgatgccatt taaaggtacg gcgctgcatc gcgggttttg catcacaggc tggcctgcag
     2101 caatgcagtg acggtcataa tggaccgtca taccagttcg ggaagcgtta ctgagccgtt
     2161 gggcatcagc aagacatctc ccgtaagttt accgccgttc agagcggcag ccaggtccag
     2221 cgcctcgtcg agggtccgga ctacgtgcat ctttgtcttt gaaaaattct cagcgccaaa
     2281 gtcagtgaca tatataaagg tatatttttc tgcggtatcg gcaaacaggt aaccggtatg
     2341 ggccccgatc gaatatttct cacgcagcgc cttctccctg tcgagcatgt tgtcataacc
     2401 ggttatctgt ttttcggtat caggcgatcc gaaaccttcc cggctctcgg acagcatgat
     2461 cattatgccg ccgggctcgg tgcattccag catatggcac agcgttttca ggggctggta
     2521 tacgtttatg tcctttggat acccgccggc cgatgcgatc actattggat agagacggtc
     2581 gatacggacc ttgttcatcc tgtcgacgag cctgcaggct tcctcgtggg cttttaccat
     2641 gtcgccagca aaggccccga ctatcctgta gctgtcgtct gcgacgacat ttattataaa
     2701 gcagcggccg agcattaggg cgccttccag catgtccaga tgcagcgggt tgccttccat
     2761 acggccgctc ttcacatcag gattgccgcc gctgccgagc ccgtggttca gcgagagcga
     2821 gtggttcgcc tgcactgtct ccctggaagc gatccccggt atgacgcttt tcctgccgcc
     2881 gctgaaaccg gcaagaaaat gatggagcac agcgccgatc aggatcacat ggtcgctgtt
     2941 gacggcgatg gaattgagct ttacaggcgt gccgcggctg gtggtgccca ggtatgtcag
     3001 gttgctttcg tccgagggat catgatccac caccctgatg cggctgtaaa tttcatctgt
     3061 gacaagtgcg cgcagttcac cggcggtttg ggcgcggtga gtgccgcgtg cgcttatgat
     3121 gacgatgtct tcgtccctta tcccggctct gttcagcctt tcaaggagga cgctcagtat
     3181 catgccgggt ttctgccagc ttcttgttac atcgctgata actatgctga ttttttcgcc
     3241 aagttttacc gtctcttcga tccgctttgc gccgatggga ttgtccagcg cttcttcgat
     3301 aagctcgcgc ggcgtacggg acggaagttc gacatggttt ggttccaaaa ccagctttat
     3361 ctgttccccg ggcaaccgca cctcgaattc acggttgcca tacttgagat agtaaggcat
     3421 ttcgatctcc ttctctatgc gtatatatgc actgcagcat acctcggtac gattgtacgg
     3481 caaacgcaat gccatgcagc actcatacgg cagaatgcgt gcaaagtatt tcattcagat
     3541 ctttgacaat aatatatcac aggcgttatg tcggcgcaat ctgcggaaga tccgggatgt
     3601 atattataac tgatcataga agaatttcga ttgtagaaat ttttcttact tctgcgcatt
     3661 tcaacgaggc gggagacatg ttcatatcgt ttgctttcga ccagtcggaa ggaagtacct
     3721 ggtagatcgg cacccgccgg ctataaaggc gggggacatc tgtcgcctcg acacagatat
     3781 gctataatat gaaatgattt ttgcctgcgg gcaagccaca gcagcatccc ggcagggagt
     3841 tcgggatgac atacggctcc aggtgcccgg agcaggtcag gctgctgaac gatagcacca
     3901 ttggccgata aaagctaata aataatgaaa ggaagtagaa aatgtacgaa ctgataatat
     3961 tcgatatcga cggtacaatg atcgacacag cggaagttgt aaaaaaatca tacagttatg
     4021 gcatgtatga ggaacttggc agggacctca ccgacgagga ggtggcaaga gccttcggag
     4081 tcccgactat ccaggcgatg gagatcctgg gagccaggaa tatcgaaaaa gcatgcggga
     4141 ggtatttcga aagccttttc aaatactaca gggaaggcgt cccgcttttt gaaggtattg
     4201 aagaattact gcaggaacta aagaggcgcg gtgtgaaatg cgggatagtc acatcgagaa
     4261 acagggacga agtggcgaac gatacgaccc ttagttcagt gatgaagtat ttcagcctta
     4321 tcgttacggc ggaagacacc aaaaaacaca agccggaagc cgagccggta ctgaaactga
     4381 tggaactggc gggtgtggtc ccggccggag tcatctatat aggagataca tattttgact
     4441 atatgtgcgc aaagaatgcc ggaatcgatt ttgcactggc atcatggggc gcaagggagg
     4501 atgacaggat caggccggac tatttgctgg ccaggccggc cgacctgctg gatatactgg
     4561 accggggccg ggagcaggac tgagcgtcgg catgatcagg gatgaatgtc atgacggaac
     4621 cggaccggtt acgaaaaccg aatcattttc tgaggctgta gccacctgaa cgaatatatt
     4681 atgcgttatt gacatattgg aaaaagaaaa ataaaataaa aataaacagc aataaacgaa
     4741 aaaggagatt cagcagtggg cgcatacgtc ataggaatag atataggaac acagggaaca
     4801 aaagctgcac ttttcgatga gaaaatggaa acgatcgcaa ctgcattcgc accatccagg
     4861 ctgatatcgc cgaaaccggg gactgtctgg caggagcctg acgacctgta tatgtcctgc
     4921 gcggcaacaa taaaagaatt gctggacaaa tcgggcgttg ctgcaaagga tgttgcggct
     4981 ataggcgtcg acggccagat ggcgggcatc atgggtgtgg atgaaaacgg agaggcgtcg
     5041 acatattacg attcgtggct cgataccagg tgcggcaaat atatggaaca gatgcgctcc
     5101 agggcaggga agagaataat cgaactgagc ggcggtcccg tttcatatgt ccacggtccc
     5161 aggatactct ggtggaaaaa cgagcacccg gaagcatatg cgaaaactgc cagattcgtc
     5221 atgccgcagg catacgttgt gagcaggatg accgggctta cggctgacga aacgtatttc
     5281 gactacactc acctgcattt ttcctgcctt gctgacaatg cgaacaagtg ctggtcggat
     5341 gaactgctgg agatatttga tatagataag aacaagatgg cgcggatcat atcgccattc
     5401 gaggtagtcg gcagggtcac ggaggatttt gcggcgatct ccggcctcgt tgaggggatc
     5461 cctgttgcag caggctgcgg agacactgcc gctagcacat tcggcgcagg aatgttcgac
     5521 aggggacagg ttttggactg cgcgggcacc gcttcggtgc tgtgcagtgt tgttgacagc
     5581 ttcgtaccgg acaccggaca tgaaaccctg acgatgatga gaagcccgat cgacggtttg
     5641 ttccttccgc tggcctatat caacggcgga ggcatgtgtg tccgctggtt ccgtgatacg
     5701 ctgagcggaa aaccggctgc tggctatgat gaactggaga aagaggctga gtctgtcgaa
     5761 cctggcagtg agggattgct ctttatcccg cacttttcgg gaagggcgct gccgtgcagc
     5821 accagcctga agggagcatg gatgggactt gatttcaagc atacccgcgg gtatatgtac
     5881 aggtcggtca tggagtcgat cgcctatgaa tacaggtatt acctgtctgt cctgcgcagc
     5941 ctgtacccaa agggcgattt cagcagcatg gtcaccatag gcggcggtgc aaaatcggcg
     6001 cttttcaacc agatcaaggc cgatgtgctc ggcatcggcg tgaccgtgtt cgaaacaggc
     6061 gatacagccc tgctgggcag tgccgttata gccgcatacg ccgtgggtat gctgacagat
     6121 tacaaacagc cgatacgccg ggtcatgaaa aaacgggcgg aatatgtttc taatgcagag
     6181 aagcacgacg cataccgttt ttatgccgat gcgtatctta gggcgatcga tcggctgaca
     6241 cctttttatg atgaactggc ttcgggagaa attggctgaa caaatcggct tttatgatcc
     6301 caacggagat atgctgcccg ttgatatcag atattttatt ttgaataaag gggtaaaagt
     6361 catggcgaaa caatccggag ctatgctggc tcaagtgcgc cagcagacca ttgctgaact
     6421 tgtccagaag atgggcaata tcactgtcaa cgacctgtgt gcgagattca acgtttctcc
     6481 cgcaacgatc aggaacgatc tgcggagcct ggaaagccgc agcattatca agcgtaccca
     6541 tggaggcgcg attagtacct ccaaaatggt atacgaactc agtaccgtcc agaaggaagt
     6601 ccggagcatc aatgaaaaaa gggcgatcgc gcggactgca gcggaattta tcgaggaagg
     6661 cgatgcgata gcgctcgatt ccggtactac gacctttgaa ctagcacgcc tgttagtgaa
     6721 cataaagaac cttactgtag ttacgaatga tttgcagata gcgtcatggc ttgaacagaa
     6781 cacctcagtg aacataatgc tggcaggcgg ccaggtgcgg cgcaatttcc gctgcacgac
     6841 aggccagtcg gccctggaaa tgctttcgat gctgcatgtt gacaaggcat ttatcgccgt
     6901 gaacggcgtg agcataaaaa gcgggctcac gacgccaacc atggacatgg cctgcataaa
     6961 gaagcaaatg atcgactcgg ccgacaaagt gatactgctg gcagacagca gcaagatagg
     7021 aaggaccgca ttcgttacct atgcgccggt ttccgtgctg gacgtgatga tcaccgacga
     7081 gtttgcagac aacagcttcg ttgtttcact gcgcagtacg ggagtgaatg tgatttgcgc
     7141 aaaggtcgat gatgctggaa gataaagaca tggtgtattt tgacataatg tttctgcagc
     7201 ggaacgtaac cagttttggc tgacatccaa atgcataaag tccctgcgat tgcatgccgg
     7261 tattatatac cggcatgtgc catttaggtg acacttctcc tgaggacact cctcagcagt
     7321 aaagaaacac cactttcaga ggagcgtcgc cattcctatt cagacatgcg atatttctca
     7381 accggaaagg aatacttctt tgccgggaag tgtcaccata ccttttacta ccgaaaataa
     7441 acgataaaaa acgataaaaa acgattgaat ctttttatgc agaagggtat aataataaca
     7501 aaaagaatac aaaagactga caggagcaga cggaaaatga gcatacgcga tttaggcatt
     7561 tgtttgatcg gatgtggacg tgccgggatg atccatgcac gcaattttgt gaacaaagtc
     7621 ccgggagcac gtatggctgc agtggtggac gtggtcgagg atactgcaaa ggctgccgca
     7681 gcggaacttg gcatcgacaa atggttcacg gattacaggc agatactgga cgacaggga
//
LOCUS       DAOSCC010000111         7666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6166_length_7666_cov_4.025752, whole genome shotgun sequence.
ACCESSION   DAOSCC010000111 DAOSCC010000000
VERSION     DAOSCC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6166_length_7666_cov_4.025752"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1064
                     /locus_tag="PLG72_07060"
     CDS             54..1064
                     /locus_tag="PLG72_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOJ80592.1"
                     /translation="MGIVGKPLKNMPWEDKPGDCQSVVWRYSGNPIIERNPTKHCGRV
                     YNSAVIPHGNGFVGIFRADHTDGKARLHFGRSSDAIHWEIEDEPIKWKDEEGKPYDPN
                     YAYDPRLVEIDGVYYIIWCTDFGGAALGMGMTRDFKEFTRLENPFPPFNRNGVLFPRK
                     INGKYMLLSRPSDSGHTPFGDIFVSESADLKYWGRHRRVMSRGGSGWWQSTKIGAGPV
                     PIETDEGWLLFYHGVTTTCSGYVYSMGAAILDRDNPSIVRYRCRDYLLTPEKLYETSG
                     FVPNVVFPCATLHDAETGRIAIYYGAADTYVALAFAQVDEVVDYIKNNSEAVYGDDIE
                     YR"
     gene            1082..2290
                     /locus_tag="PLG72_07065"
     CDS             1082..2290
                     /locus_tag="PLG72_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOJ80593.1"
                     /translation="MLSTTRQLKDHVENELRTNILDFWCDHAVDNENGGFYGYISEDL
                     STDALHDKASVLNARILWTFSAAYGSYKEKKYLDMAARAFEYIRDHFINREHMGVYWM
                     LDYRGMPKDTKNQVYANAFAIYGLSEYYRAVGDKSALELAISLFESLERHARDREYGG
                     YLEALACDWSPLPDMSLSPKDMNVPKSMNTHLHVLEAYTNLYRVWKSEELKESLKALL
                     EDVMDRIVDNHTWSFRLFFEKDWTPCAEITSYGHDIEGSWLIHEAAEALGDHALLEKA
                     GKTAVNMAESVLENGVDKKFGGIFNEMHGTILDDNKDWWPQAEAVVGFFNAWQLTRKG
                     VFLDEALKTWKFIDMYIADRNNGEWRRTVARNGSPLAGDEKVSPWKCPYHNGRMCLEI
                     IRRTAGCETG"
     gene            2366..3565
                     /locus_tag="PLG72_07070"
     CDS             2366..3565
                     /locus_tag="PLG72_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOJ80594.1"
                     /translation="MSSYFKNRLEQLIAEYNSIITRRNEPLPSGNGIYERFRYPVLTK
                     DHIPLFWKYDLNEGTNPYLMERLGVNSVFNPGAVELDGKIYLIARVEGNDRKSFFAVA
                     ESENGIDNFRFWDYPVIMPETDDPDVNVYDMRITKHEDGWLYGLFCTERKDPDAPPGD
                     TSSAVAACGIARTRDLKTWERLPDLKTKSPQQRNVVLHPEFVNGKYAFYTRPQDGFIE
                     TGTGGGIGWALSDMIENASIDDEQLIDCKEYHTIKEVKNGQGPAPIKTEKGWLHIAHG
                     VRNTAAGLRYVIYAFLSDPEHPEIVTHRPGGYLIAPEGDERVGDVSNVVFCNGAVARA
                     NGELLIYYASSDTRVHVARTTVDKMLDYVINTPEDPLRSYACVQVRKELIDRNMELIK
                     SGKIKLS"
     gene            complement(3776..4321)
                     /locus_tag="PLG72_07075"
     CDS             complement(3776..4321)
                     /locus_tag="PLG72_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOJ80595.1"
                     /translation="MKLRSKFAAATLMLVLVISLSMNIVSALNQTAIPGSDQDPIVSK
                     SYVDAAFEQLSAKIQALLDKNNELTNKIAEQEKQIKALQTEINTLKSGAAAGPAQSGS
                     GSSGDGKAPEQPAAQLKGVVNVAALNLREKPSTSARKLGTLYKNETVTILSDEGNGWY
                     KIKTSKGTTGYVFAIYVTVKK"
     gene            4571..5956
                     /locus_tag="PLG72_07080"
     CDS             4571..5956
                     /locus_tag="PLG72_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ80596.1"
                     /translation="MQEKTGLEQDLGIFGGREFRKRFLSIAVPVTLQQFLTSSLNLTN
                     NILVGQLGDTSVAAVGLANQVYFILNVILSGIGGGISIYISQFWGSRKYDQIKKVVSL
                     SLAITMLAAMVFFIPSFAAPGFILSLFSNDTGVIELGSKYLKPVSISFLLMIFSVCFS
                     SALRSTGNVRLPLAANFVGIIVNTALNYVLIFGKLGLPAMGVSGSAVATVIARFAESA
                     IILWAVYASHNIVSIKLKDLFSISADLIKRFFGTSGELIAKDGCWALGTSIYMAIYAL
                     MSTEAAASVNITSTIRSMLFVFFNGVSTACLIMVGNKIGENDDETAYKYACRFLRITL
                     LISAAVAAILVLSRPLVLAPYKVSQTVVDNAMGLMVVSAVIIPFQSYASVAIVGAMRG
                     GGDIRFTIFLDLTAVWFIGMPLAWLGGMVFRLDIVWVFLLVSLQEVYKAVMCYFRIHS
                     RKWINNVVRGI"
     gene            complement(5918..6445)
                     /locus_tag="PLG72_07085"
     CDS             complement(5918..6445)
                     /locus_tag="PLG72_07085"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOJ80597.1"
                     /translation="MRIHSFEPIADENCKVLILGTMPSVVSLERQQYYGFGRNAFWKV
                     IYAVFGREPDEDYELRKAFLLGHRIALWDVLESCEREGSSDSDIRNPEPNDFEGFFKR
                     FKGIRYVCFNGNPARDLYSRLVRRKGTGNHIAQYFVLPSTSPRNMLPFEKKLEKWMLV
                     RELLDPADHVVDPLP"
     gene            complement(6460..7092)
                     /gene="yihA"
                     /locus_tag="PLG72_07090"
     CDS             complement(6460..7092)
                     /gene="yihA"
                     /locus_tag="PLG72_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOJ80598.1"
                     /translation="MDTGRSGQMDIGRSRYELTAVKPEQYPAGDLPEIALVGRSNVGK
                     SSLINSLLNRKGLARVSSEPGKTRGINFYNVDDILYFVDLPGYGYAKVSKEEKASWAK
                     MIETYLHTRRQLKLVIMLVDIRHSPTEDDKLMYRWILDRGIAHMVVATKADKIARGRI
                     QGRFSEIRKTLGIKADASLTAYSSETGQGREQLWSELEKAVLEQTVNEQI"
     gene            complement(7252..>7666)
                     /locus_tag="PLG72_07095"
     CDS             complement(7252..>7666)
                     /locus_tag="PLG72_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOJ80599.1"
                     /translation="RVSGNEAHWVKREDRDAGYESFRKYLDTVFAYAGTMSLSKEMKK
                     TDPQGLEPGDVFLKGGSPGHCAIVVDMAENPGTGEKVFIIAQSYMPAQDIHILKNPSK
                     GDGDPWYPADFGDTLVTPEWTFTADQVYRFADADM"
BASE COUNT         1917 a         1978 c         1956 g         1815 t
ORIGIN      
        1 aatataatat tacaaacaat aacttgtaat gttacatcca ggaggctgta agcatgggta
       61 tagtaggcaa gccattgaaa aacatgccct gggaagataa accgggcgat tgtcaaagcg
      121 tagtgtggag atactccgga aatccgatca tagaacgcaa tcccacaaag cattgcggga
      181 gagtgtacaa cagcgcggtg atacctcatg gcaacgggtt cgtcggaata ttcagggctg
      241 accacacgga cggcaaggcc aggctccatt ttggccgcag cagtgacgcc atacactggg
      301 agatcgaaga tgaacccatc aagtggaaag acgaagaagg aaagccgtat gatcccaatt
      361 atgcatatga tcccaggctg gttgagatcg acggcgttta ttacataatc tggtgcaccg
      421 atttcggcgg tgcagcgctc ggcatgggca tgaccaggga tttcaaagag ttcaccaggc
      481 tcgagaaccc cttccctcct ttcaaccgga acggcgtcct cttccccagg aagataaacg
      541 gaaaatacat gctgctcagc agacctagcg acagcggcca cacaccgttc ggcgacatat
      601 ttgtcagcga aagtgcagac cttaagtact ggggaaggca caggcgcgtc atgtcaaggg
      661 gaggcagcgg atggtggcag agcacgaaaa taggtgcagg ccctgttccc atcgagacag
      721 acgaaggatg gctgctcttc taccacgggg tcaccacgac atgcagcggt tatgtttaca
      781 gcatgggcgc cgctatactg gacagggata acccttctat agtaagatac aggtgcaggg
      841 actacctgct gacgcctgaa aagttatatg aaacctcggg tttcgtacca aacgtcgtat
      901 tcccgtgcgc taccctgcat gacgctgaaa cgggcaggat agctatctac tatggagcag
      961 cagataccta tgtcgccctt gcatttgctc aagtggacga agtagttgat tacataaaga
     1021 acaactcgga agcagtttac ggtgacgata tcgaatacag gtaaccagag gtgcggcaac
     1081 aatgcttagt actaccaggc aactgaagga tcatgtcgag aacgagttga gaacgaatat
     1141 cctggacttc tggtgcgatc atgctgtcga caatgaaaac ggaggctttt acggctatat
     1201 ttccgaggat ctcagtacgg atgccctgca tgacaaggca tccgtactaa atgcccgcat
     1261 attgtggaca ttttcggcag catacggttc atacaaagag aagaaatacc tcgacatggc
     1321 agcgcgcgca ttcgagtaca tccgtgatca cttcataaac cgcgaacaca tgggtgtata
     1381 ctggatgctc gattacaggg gcatgccgaa agatacgaaa aaccaggtat atgccaatgc
     1441 atttgccata tacggcctgt ctgaatatta ccgtgcagtc ggcgataaat ccgcgctgga
     1501 actggcaata agcctgttcg agtccctgga acggcatgca agggacaggg aatacggagg
     1561 ttacctggaa gcgctggcct gcgactggtc ccccctgccg gatatgagcc tcagtcccaa
     1621 ggatatgaac gtgccgaaat cgatgaacac gcacctgcat gtcctggaag cttacacgaa
     1681 cctgtacagg gtctggaagt ccgaagaact gaaagaaagt ctcaaagcgt tgctggaaga
     1741 cgtaatggac cgcatagtcg acaaccacac ctggagtttc aggctgttct tcgaaaagga
     1801 ctggactcca tgcgcagaaa tcacatccta cgggcatgac atagaaggaa gctggcttat
     1861 ccatgaagcc gccgaggcgt tgggagatca cgctctcctg gaaaaagccg gaaaaaccgc
     1921 tgtcaatatg gccgagagcg tccttgagaa cggcgtcgac aaaaagttcg gcggtatatt
     1981 caacgagatg catggtacga tactggatga caataaagac tggtggcccc aggcagaagc
     2041 agtcgtcggt tttttcaacg catggcagct cacccgcaag ggggtgttcc tggacgaagc
     2101 acttaaaaca tggaagttca tcgatatgta tatagcagac cgtaacaacg gagaatggcg
     2161 ccggacagtc gcgcgtaacg gcagtcctct tgcaggcgat gagaaggtaa gcccatggaa
     2221 gtgcccctac cataacggca ggatgtgctt agagatcatc aggagaaccg ccggatgcga
     2281 aaccggatga cagcgcctgt ccgggagata ttctgaaagt atgaaaacgg ccgggagcaa
     2341 aatctttcag gaggcaggat caaatatgag cagttacttc aaaaacagac ttgagcaact
     2401 catagctgaa tacaacagta taatcaccag gaggaacgag cctcttccgt caggcaacgg
     2461 gatatacgaa aggttcaggt atccggtgct gacaaaagac catatcccgc tgttctggaa
     2521 gtacgatcta aacgagggaa cgaatcccta cctgatggaa aggctgggcg taaacagcgt
     2581 attcaacccc ggggcggtcg aacttgacgg gaagatatac ctcatcgccc gcgtggaagg
     2641 caatgacagg aagtcttttt tcgcagtggc agaaagcgaa aacggcatag acaacttcag
     2701 gttctgggac taccccgtca tcatgcccga gaccgatgat ccggacgtca acgtgtatga
     2761 catgaggatc acgaagcacg aggacggctg gctatacggc ttgttctgca cggaacgcaa
     2821 agatccggac gcgccgcccg gcgatacctc gagcgctgtg gcagcatgcg gcatagccag
     2881 gaccagggac ctcaagacct gggaacgcct cccggacctt aagacgaaat caccgcagca
     2941 gaggaacgtg gtgctgcatc ccgagttcgt aaacggcaaa tacgccttct acacaaggcc
     3001 ccaggacggt ttcatagaga ccgggaccgg cggcgggatc ggatgggccc tttccgatat
     3061 gatcgagaac gcttccatcg atgacgaaca actgatcgac tgcaaggaat accacaccat
     3121 aaaggaagtc aagaacggcc agggcccggc gccgataaaa acggaaaaag gctggctcca
     3181 tatcgcacat ggcgtcagga acaccgcagc cgggctgcgt tacgtgatct atgccttcct
     3241 gtccgatcct gagcatcccg agatcgtcac ccacaggccc ggcggctacc tgatagcccc
     3301 cgagggcgat gaacgggtgg gagacgtgtc gaacgtggta ttctgcaacg gcgctgttgc
     3361 cagggccaat ggcgagttgc tgatatacta tgcttcctcc gacaccaggg tccatgtggc
     3421 acgtaccacg gtcgacaaaa tgctcgatta tgtaatcaat acgccggaag atcctcttag
     3481 atcgtatgca tgcgtgcagg taagaaaaga actcatcgac aggaacatgg aactgataaa
     3541 gtcaggtaaa ataaagctgt cctgagacaa tgactgatgg atcgtgttac cggcggccat
     3601 atataccccg ggtgatcaat gtcctgtgta cggaaacaca aaactgtaca ggaaatcaaa
     3661 acagcaatcc gcataaacaa agctgcagca gcatgtaaag ctgttgcagc ttttgatatc
     3721 tttcccgttt ctttatgctt tccgtacaat tggtaccgtt tcattccagt ccagtctatt
     3781 tctttactgt cacgtatatt gcaaatacat atcccgtcgt cccttttgac gttttgatct
     3841 tgtaccatcc gttgccctca tccgagagta tcgtgacagt ctcgttctta tacagcgtcc
     3901 caagtttgcg cgctgatgtg ctgggttttt cccgcaggtt gagcgcggcg acattgacga
     3961 cgcctttgag ctgcgcagca ggctgttccg gagccttccc gtcaccgctg ctgccgctcc
     4021 ccgactgtgc agggcccgca gcggcgcctg atttcagcgt atttatttct gtctggagcg
     4081 ccttgatctg cttttcctgt tcagctattt tattggtcag ttcattgttc ttatccagaa
     4141 gcgcttggat cttggcggac aattgttcga acgcagcatc aacatagctt ttgctcacta
     4201 tgggatcctg gtccgaaccg ggtatggcgg tctggtttaa ggcagatacg atattcatgc
     4261 tgagtgaaat gaccagtacc agcattagag ttgctgctgc gaatttcgac cgaagtttca
     4321 tgacttgccc cctcttcatt cgtttagtgc tcataatccg agcacattat cattataacg
     4381 caatattgaa gatttttggt tacagaatcc ttaaaaatgc gtgacattat tcaaaaaaag
     4441 agcaatttat gaggacctgc gcgcaaaatc tggcgagcaa aaatgcccgc acgacttata
     4501 attaatgtgc ctggtataat cgatggaata ttcggcggca taactttcac aacaggacgg
     4561 agatcacagg atgcaggaaa aaacaggttt ggaacaggat ctcggtatat tcggcggcag
     4621 ggaattccgg aaaaggttcc tcagcatagc ggtacccgtt acattacagc aatttctgac
     4681 ttcatcgctg aacctgacga acaatatcct ggtcgggcag cttggcgata caagcgtggc
     4741 tgcagtggga ctggccaacc aggtttactt catactcaat gtcatactgt ccggcatagg
     4801 cggaggcatc tccatatata tatcccagtt ctggggcagc cgtaaatatg accagataaa
     4861 gaaagtggtt tcgctcagtt tagctattac gatgctggcc gcaatggtat tcttcatacc
     4921 gtcgttcgcg gcgccgggat tcatactgtc cctgttttcc aatgacaccg gcgtcatcga
     4981 actcggaagc aaatacctga aacccgtgag catcagtttc ctgctgatga tcttctcagt
     5041 ctgtttttcc agcgcactgc gcagcacggg aaatgtgagg cttccgctgg cagcgaattt
     5101 cgtcgggata atcgtgaata ccgcattgaa ttatgtgctg atattcggca agctcggact
     5161 gccggccatg ggtgtttcag gatctgccgt cgccacggta atagcccgtt tcgctgagtc
     5221 ggccatcatt ttgtgggcgg tatatgcctc ccacaatata gtatcgataa agctgaagga
     5281 tctgttcagc atcagcgccg acctgatcaa aagattcttc ggcacttcgg gcgaactgat
     5341 agcaaaagac ggctgttggg ctctaggcac atccatttat atggcgatat acgcattgat
     5401 gagcaccgaa gccgcagctt ccgtcaacat aacatcaacg atacgcagca tgcttttcgt
     5461 attctttaac ggcgtttcca cagcatgcct gataatggtc ggcaacaaga taggcgaaaa
     5521 tgacgacgaa acggcgtaca aatatgcctg caggttcctc aggataactc ttcttatttc
     5581 agcggcagtc gcagcgatcc tggtcctttc gaggccgttg gtgctggctc cgtacaaggt
     5641 gtcgcagacg gtcgttgaca acgccatggg gctgatggtc gtttccgccg ttatcatccc
     5701 gttccagtcc tacgccagcg tagccatagt cggagccatg agaggcggag gcgacataag
     5761 gttcaccatc ttcctcgatc ttaccgccgt atggttcata ggcatgcccc tggcatggct
     5821 cggcggcatg gtgttccgtc tcgacatagt gtgggtcttc ctgctggtaa gcctccagga
     5881 agtatacaag gcagttatgt gttatttcag gatacattca aggaagtgga tcaacaacgt
     5941 ggtccgcggg atctagaagt tccctgacca gcatccattt ttcgagcttt ttttcgaaag
     6001 ggagcatgtt cctggggctc gtggacggaa ggacgaaata ctgtgcgata tggtttcccg
     6061 tccctttcct tctgacaagc ctgctataca gatcccttgc agggttgccg ttgaagcata
     6121 catacctgat acctttgaac cgtttaaaaa agccctcgaa atcattaggc tcgggattcc
     6181 taatatcgct gtcgctgctg ccttcccttt cgcatgattc cagcacgtcc cagagcgcta
     6241 tacggtgtcc gagcaggaat gccttgcgca gttcatagtc ttcgtccggc tccctgccga
     6301 aaacagcata tatgaccttc cagaacgcat tgcgtccgaa tccgtaatac tgctgcctct
     6361 ccagcgaaac aacgctcggc atggtcccta gtatcagcac tttacagttc tcatcggcga
     6421 tgggttcgaa tgaatgtatc ctcatatcgt ttactgtcct catatctgtt cattgaccgt
     6481 ctgctcaagc acggctttct caagttcgct ccagagctgc tcccttccct gcccggtctc
     6541 tgacgaatat gcggtaagac tcgcatcagc cttgatcccc agtgttttcc ggatctccga
     6601 aaaccttccc tgtatccgtc ccctggctat tttgtcggct ttcgttgcga ccaccatatg
     6661 ggcgatcccg cggtccagta tccacctgta catcagcttg tcatcttcgg tgggagaatg
     6721 ccttatatcg acaagcatga tcaccagctt gagctgcctt cgtgtatgca gataggtctc
     6781 gatcattttt gcccaggacg ccttttcctc ttttgaaacc tttgcatagc cgtatccggg
     6841 gagatcgaca aaatacagga tgtcatccac attgtagaag tttatgcccc gagtcttgcc
     6901 gggctcggaa gataccctgg ccaggccctt cctgttcagg agcgagttga tgagcgacga
     6961 tttccccaca ttcgaccttc cgaccagggc tatctcaggc aggtcgcctg cagggtactg
     7021 ttcaggcttc acagccgtaa gttcgtacct ggatctgccg atgtccattt gtccggatct
     7081 gcctgtgtcc atgatgcctc cctttgtcag ctttctcttt ctttcgcggt cgtataattt
     7141 attatactaa aaaagccggt ttttacaaat catctgtcaa aactggctgt attattcctt
     7201 tttcagcgta tttccgcctt gctgactgcg gcacggcgcc gccatatatt atcacatatc
     7261 ggcatcagca aacctgtata cctggtctgc cgtgaacgtc cactccgggg tgaccagcgt
     7321 atcgccgaaa tctgcgggat accacgggtc gccgtcgcct ttcgaaggat tcttcaggat
     7381 atgtatgtcc tgtgcgggca tatagctctg tgcaatgata aataccttct caccggtacc
     7441 aggattctcc gccatgtcca caacgatagc gcaatgccct gggctgcctc ccttcaggaa
     7501 cacgtcgccc ggctccagac cctgcgggtc cgtctttttc atttctttcg aaagggacat
     7561 cgtgcctgca taggcgaata cagtgtcaag gtatttcctg aaagactcat agccggcgtc
     7621 cctgtcctct cgttttaccc aatgggcttc gtttcccgat accctt
//
LOCUS       DAOSCC010000112         7652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6183_length_7652_cov_5.435830, whole genome shotgun sequence.
ACCESSION   DAOSCC010000112 DAOSCC010000000
VERSION     DAOSCC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_7652_cov_5.435830"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1199
                     /locus_tag="PLG72_07100"
     CDS             279..1199
                     /locus_tag="PLG72_07100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ80600.1"
                     /translation="MPQINSIYQFCEDPPVKLSGENQYISYDGIGEDFEPQVIFWDAN
                     NIERYGVYEACGDQIARKFGRNITVLDLGAGLGTASIPLAKNGIHTITADISNVMLST
                     GVKRAKGRYSNLIFARMNAYEIMLADSSVDIIIENAVLHLVSNPESVIKEMVRVLKPG
                     GCLIRYSTYGQPVSEEEAKQNQYCNSVLEDISSKYFDYLKEHGYKSIWFDNHFEDIIK
                     NYFKPPCRDIVENFSEVFTEKMKFRLHRLKTGAHSDLQDTPRGLINAAWQYTDSYAVK
                     KYGPGYMEIKGFSRYGAAIDIYELNGSKAQ"
     gene            complement(1470..3431)
                     /locus_tag="PLG72_07105"
     CDS             complement(1470..3431)
                     /locus_tag="PLG72_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOJ80601.1"
                     /translation="MPTVSVAIPDTTFVTSSHPAQNFSFYPLMYVGNDAGFMNCISLM
                     EIDLPPLPVTTVDKAVLKLSVIVKTGTDPSPIVVNRVTSPFDVAAVTFNTLPDYETTT
                     SQIDIDISDLYTIIEIDITELVNQWLNGIYPNYGIALTNPDGVTLVQFGTNNIVYEPY
                     FPKLEITYSDTPVPPPTGNAYGYIYDTTEQIVPQNADVKFGNNGALLGIIHETGTAPI
                     IIESAGIYAVWYTVIASNHNQFTLFRNGNPVPGSTNGTENTYTPYSGMVIINAAAGDQ
                     LTLRNYTSALPGSVSIVSRVGGERTGISASVLILKIGEAITPDPKLDAVNSAQNITQM
                     RAAITDPALGLDLDGFNSLSTAAQDTVLGVLLASRPDLGYQTVESLQETLDEAVNETV
                     DPENIRVQAGSVDGNGSIAHPFGSISEGIAAVAPGGTVHVAAGTYSVTTQINFNKAGA
                     TLFGEPGAVIYLQAEVIPLLITGAGATVQGLTMTSDVPYTGEFIQIGAPNVKLIGNTI
                     YGPEQPPPMDNWTVNRAVVSQVNTINVLLEGNTFYSLRTGMYINPGTTGAINNNVVYN
                     TKGGFLVDRAFTTFFGNSWGDPPNEFDIVLLAGTTDGPPYDDLARLSEENDNANISDQ
                     RGTGGNGGGSDEPGEPDEPGEPVEPGESI"
     gene            3998..5023
                     /gene="gmd"
                     /locus_tag="PLG72_07110"
     CDS             3998..5023
                     /gene="gmd"
                     /locus_tag="PLG72_07110"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ80602.1"
                     /translation="MNKALITGITGQDGSYLAEFLLDKGYEVHGLVRRSSTDNTGRIS
                     HLLNGPKKVTLHYGDLTDSGSLSRLLYRIEPTEIYNLAAQSYVKVSFDIPEFTADVNA
                     VGTVRLLESIREVNPGIKFYQASSSELFGKVRETPQNEKTPFYPRSPYAVSKLYAYWI
                     TVNYREAYNLFACNGILFNHESPRRGKNFVTRKITTGIADILAGKLDCLCLGNLDSKR
                     DWGFAGDYVEAMWLILRQNEPDDYVVATGETHTVREFCELAFKCAGMDLVWEGKGLDE
                     KGIDNATGKVLVRVSSEFFRPTEVDLLLGDPTKANSKLGWKPRVSFEELVAMMVKSDL
                     DAAGIKL"
     gene            5117..6064
                     /locus_tag="PLG72_07115"
     CDS             5117..6064
                     /locus_tag="PLG72_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOJ80603.1"
                     /translation="MKKSSRIYVAGHTGMVGSAIVRCLLQNGYENIIKRTHQELDLTN
                     QLDTEKFFEEEKPEYVFLAAAKVGGIHANDTYPADFIMENMLIECNVIRSAFKYNVKK
                     LMFLGSSCIYPKLCPQPIREEYLLTGALEPTNEAYALSKIAGIKMCQSYNKQYGTRYI
                     SVMPANLYGVNDRFDAYNSHVIPSMIIKFHRAKCEDRPFVELWGTGKPLREFLYVDDM
                     AEACLWLMQNYESSELINIGSGNEISIRELAEIIREVVGYQGEIVFDTSKPDGTPRRV
                     LDNSKISATGWKPKVDIKEGIRREYEYYLSTHVGDSSSE"
     gene            complement(6350..6802)
                     /locus_tag="PLG72_07120"
     CDS             complement(6350..6802)
                     /locus_tag="PLG72_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ80604.1"
                     /translation="MNYTSADLKRVTLKKALFDGYSKKQVDTLLAKIIEDYAEMSNSI
                     NQLKCEITSLNEAVQHYKVLEESLQHSILVAQHTAEQIKANACDKAKNIVDEAEIKAQ
                     KIIDEAAEKVRNIQVRYEQLKAEVYTFKTKSEALIKAQMDVLSQLLAE"
     gene            complement(6862..>7652)
                     /locus_tag="PLG72_07125"
     CDS             complement(6862..>7652)
                     /locus_tag="PLG72_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ80605.1"
                     /translation="KYDWSAVYLHNEAEVLGFIRNFIALPLPDVEIVDQNVTKREYIK
                     GSPLYSNVLLKSDKSTQDTVAREIAVFLEQLHSIPVKKAKNTRIGDSRMNLGREEWLA
                     EIETLERKIFPYCTGYVKEYFRQITQPAVDNEEFFNFQPVLIHGELVPCHILVNNAAL
                     GISSIIGFSNAGFGDPAYDIAMILDHLGEGFVRRISRYYPITAECMDRARFYAYISSF
                     MWYRDVCDMITTRDFSRFQIQVKGRDISPIGAPVMVGGIKPSKK"
BASE COUNT         2075 a         1776 c         1735 g         2066 t
ORIGIN      
        1 atcggcttcc gtggaattta cttttgcaaa gcggaaatta ccttttcaat tgacactggt
       61 gaaatgtacc aatccattca aagctactcg cggccctgtc cgataaaagt aaacatctga
      121 agaaatgtcc ccgagtttcc agcttttgac tatacagctc tacataatgt ataatttaca
      181 cgaggggtga taaaatgtcc gtttcggata acatatttct atgcccgaag tgcaggaaaa
      241 accttgcagt atttaaatgc accggatgcg gatatgaggt gccgcagata aattctatct
      301 atcagttctg tgaagatccg ccggtaaagc tcagcggaga aaaccagtat ataagttatg
      361 acggaatcgg tgaggatttt gagccgcagg ttatattctg ggatgcaaac aacatcgaac
      421 gttacggtgt atatgaagca tgcggcgacc agatagcacg gaaattcggc aggaacatca
      481 cggtactcga tcttggagcc ggactcggca cagcatccat acctcttgcc aaaaacggga
      541 tccataccat tacagccgat atttccaacg tgatgttgtc caccggtgtc aaaagagcca
      601 aagggcgtta tagcaacctg attttcgcac ggatgaacgc ctacgaaatc atgctggctg
      661 acagcagcgt ggatattatc atcgaaaatg ccgtgcttca tctcgtaagc aacccggaaa
      721 gcgtgatcaa agaaatggta cgtgttttaa agcctggcgg gtgtcttatc cgctacagca
      781 catacggcca gccggtatcc gaagaggagg caaagcagaa ccagtactgc aacagtgtac
      841 tcgaagatat cagcagtaaa tattttgatt atcttaaaga gcatggctat aagagcatct
      901 ggttcgataa tcattttgag gatattatca agaactattt caagcccccc tgccgtgata
      961 tagttgaaaa cttttccgaa gtctttaccg aaaaaatgaa attccgcctg cacaggctta
     1021 aaaccggtgc tcattcagat ttacaggata caccgagagg cttgatcaac gccgcatggc
     1081 aatataccga tagttatgcg gtcaaaaaat atggacctgg ctatatggaa atcaaaggtt
     1141 tctcccggta tggcgcggcc attgatatat atgagctgaa cgggagtaaa gcacaataaa
     1201 tgagcatttc tatccgttgt ctcgcaaaac tgactgccat ctataaactg caacagtttt
     1261 tgtaacagag ggctgccctg cttccagggc agccaactct aaagtattcc gcatcagtgc
     1321 atttcatgta ttcatgcttc tcataattgt cgcgtatttt tgttatcatt atttatgtgc
     1381 tcatggcctg atgatttcat tctcatagtt cttatgggtc atatatgttt agcggctgct
     1441 ggggctactg gctccacgcg ctattagggt tatatcgatt cccctggttc tacaggttct
     1501 cccggttcat ctggttctcc cggttcatcg cttcctccgc cgttaccgcc ggttcctctc
     1561 tggtcggaaa tattcgcgtt gtcgttttcc tcagacaatc ttgcaagatc gtcgtacggc
     1621 ggtccatcag ttgtacccgc aaggagaact atatcaaatt cgttgggcgg atctccccac
     1681 gagttcccga aaaatgttgt aaacgcacgg tctaccagga atccgccttt tgtattgtaa
     1741 accacattgt tattgatggc gcctgtcgta ccagggttga tatacattcc ggtcctcagc
     1801 gagtagaaag tatttccctc cagaagaaca tttatcgtat tcacctgtga tacgactgca
     1861 cggttgactg tccaattatc catcggcggc ggctgctcag ggccgtagat tgtattccct
     1921 atcagtttta cattcggtgc gccaatctgt atgaactcac cggtataagg aacatcactg
     1981 gtcatcgtca gtccctgtac agtcgcacct gcacccgtga tcagcagcgg gattacctct
     2041 gcctgcaggt agatcaccgc tcccggctcg ccaaacagtg ttgcgccggc tttgttaaaa
     2101 ttgatctgag ttgtaacgct gtatgtgccg gccgctacat gtaccgtccc tccgggagca
     2161 actgcggcaa taccttcgga aatgctcccg aacggatgcg cgatgcttcc attcccgtca
     2221 acgctgccgg cctgcacgcg gatattttca ggatcgacgg tttcattcac cgcctcgtca
     2281 agcgtttcct gcaggctctc cactgtctgg tacccgagat cgggcctgct tgcaagcaga
     2341 actcccagca ccgtatcctg cgctgctgtg ctcaatgagt taaacccgtc aagatcaagt
     2401 ccaagagcag gatcagttat agctgcccgc atctgtgtta tattctgtgc actattcaca
     2461 gcatccagct tcgggtcggg agttattgct tcaccgattt tcagtatcag gacagatgca
     2521 ctgataccgg tcctctctcc acctactcta gagacaatgc ttacagaacc aggcaatgcg
     2581 ctggtatagt ttctcagagt aagttggtcg cctgcagcag cattgataat taccatcccg
     2641 gaataaggag tgtaagtatt ttctgttcca tttgtgcttc ccggcacagg attgccattc
     2701 ctgaacagtg taaactggtt gtgatttgac gctataacag tataccagac agcatagata
     2761 ccagcagact ctatgataat aggcgctgta cctgtctcat gtatgatacc caataaagct
     2821 ccgttgttgc cgaatttaac atctgcgttt tgtgggacta tctgttcggt ggtgtcatat
     2881 atatacccat aagcattgcc ggtcggcggc ggaacaggtg tatctgaata ggtaatctcc
     2941 agtttcggga aatacggctc ataaactatg ttattcgtac cgaattggac aagggtaacc
     3001 ccatccggat tcgtcaacgc gatcccgtaa tttgggtaaa ttccgttgag ccactggttc
     3061 acgagttctg taatatctat ctcgattatc gtatacaaat ccgatatatc gatatcgatt
     3121 tgtgaagttg ttgtttcgta atccgggagt gtgttgaagg ttacagcggc aacatcaaac
     3181 ggactggtta ccctgttaac aacaatcgga cttggatctg tacctgtctt cactatcaca
     3241 gataatttca gcaccgcttt gtcgacagtt gttaccggca aaggcggcag gtcgatctcc
     3301 ataaggctta tgcagttcat aaagcccgca tcatttccta cgtacatcag tggataaaag
     3361 gagaaatttt gcgccggatg agatgatgtc acaaatgtag tatcgggtat agcgaccgat
     3421 actgtcggca taatcagtca cttcctttct tggagttata tacttttgaa gcacgaaata
     3481 cctggtctca gaatcaggca attcatcacg cttccccatc attacatcgt attcttgcat
     3541 atgcaggatg ttacatacaa aagcgaagtg cttcaaatat cctgcgtttt tacgtgtccc
     3601 gatataattg ctgtctttgc acacatgttc tgcccttgca cctttggtcc catactgctt
     3661 cttctgtcat tgccttctct ggttgcctgt actgcctgct tctgcttaag ggtacgaaaa
     3721 tggaacatta cataatcagt ttgcatatta ttattccgag accacttatt ggcttcagcg
     3781 atttgacaat ccggagcatg catatacgga acagagcacg aagagaagaa ttccgtgatg
     3841 ccggtttgct gccgggccga ggtaatgttg atgatagttg atatgatttg tcgcaaataa
     3901 aactgatcaa agcaatatac ttgaaaaatg tgtactccgc taatcaagct atcggataaa
     3961 ggtcaaaaat tcatcaatgc cataggaggg tatccgaatg aacaaggcat taataacagg
     4021 aataaccgga caggatggtt cttatctggc ggaattcttg ctggataaag gttatgaagt
     4081 tcacggactc gtccgtagaa gcagcacaga caatacagga aggatcagtc acctgctcaa
     4141 cgggccgaaa aaagtgactc tgcactacgg tgatttgacc gactcgggca gcctgagcag
     4201 gctgctgtac aggatagaac cgactgaaat atataatctg gcagcccaga gttatgtgaa
     4261 agtttccttt gatataccgg aatttacggc agatgtaaat gctgtcggca cggtcaggct
     4321 gctggaaagc atccgtgaag taaatccagg aatcaagttt taccaggcat catccagcga
     4381 gttgttcggg aaagtacggg aaacaccgca aaatgaaaaa acgccgttct atcccagaag
     4441 tccatatgca gtaagcaagc tttacgcata ctggattacc gtcaactaca gggaagcgta
     4501 taatctcttc gcctgcaacg ggattctgtt caatcatgag tctccaagga gaggcaaaaa
     4561 ctttgtaacc agaaaaataa cgacaggtat cgccgacatc ctggccggga aacttgactg
     4621 tctatgcctt ggaaatctgg attccaaaag agactgggga ttcgccggtg actatgttga
     4681 agccatgtgg ctcatactcc ggcaaaatga gcccgatgac tatgttgttg caaccggtga
     4741 aacacatacg gtaagagaat tctgcgagct ggcgttcaaa tgcgccggga tggacctggt
     4801 ctgggaaggc aaaggactgg atgaaaaagg tatcgataat gccacaggca aagtgcttgt
     4861 cagagtcagc agcgagtttt tcaggcctac agaagtggat cttcttctgg gggatccgac
     4921 gaaagcaaat agcaagcttg gctggaaacc aagagtatca ttcgaagagt tggtagcaat
     4981 gatggttaag agcgacctcg atgcagcagg cattaagctg tagcaggaca cagttataaa
     5041 actgcaacag taagcaggcg aactgcgatg aagcagcaaa gaaatatagc aaaacagtat
     5101 ttcaggaggg attcacatga aaaaaagcag ccggatatat gttgcaggcc acacaggaat
     5161 ggttggatcg gccattgtca gatgtcttct gcagaacgga tatgagaaca tcataaaaag
     5221 gacgcatcag gagttggacc tgacgaacca gctggatacc gaaaagtttt ttgaagaaga
     5281 aaaacccgag tatgttttcc tggctgcggc aaaagttggg gggatccatg caaacgatac
     5341 ttacccggcg gatttcatca tggaaaatat gctgatcgaa tgcaacgtta tccgcagcgc
     5401 tttcaagtat aatgtcaaaa agcttatgtt ccttgggagc tcatgtattt atccgaaatt
     5461 gtgtccccag ccgatcaggg aggaatacct gctcaccggt gcactggaac cgaccaacga
     5521 agcatatgcc ctgtccaaga tagccgggat caaaatgtgc cagtcataca acaaacagta
     5581 cggcacccgc tatatctctg tcatgcctgc aaacctttac ggcgtcaatg acaggtttga
     5641 tgcatacaac tcgcatgtga tcccttcgat gataataaaa tttcacaggg cgaaatgtga
     5701 ggaccggcca tttgttgaat tatggggaac cggcaaacca ttgcgtgaat tcttatatgt
     5761 cgatgacatg gccgaagcat gtctctggct tatgcagaac tatgaaagca gcgagctgat
     5821 aaacatcggt tcagggaatg agatcagcat aagggaactg gccgaaatca tacgtgaagt
     5881 tgtcggctat cagggagaaa tcgtattcga tacttcaaaa cctgacggaa cgccgcggcg
     5941 agtcctggac aactcgaaaa tctcggcaac cggctggaaa ccgaaagttg atataaaaga
     6001 aggcatccgc agagaatacg aatactacct cagcacccat gtcggggaca gttcttcaga
     6061 atgagtggct gagggacagt tctcaaatat tgggggtagt cattcagaat gagtggctga
     6121 tgcagttcat caaatccttg ggacagttcg tcagaatatg ggatcatttt cgtaacttgg
     6181 ggacagttct tcaaatctta agaactgttc atctaaaaga tatcaattct tcatgggaaa
     6241 gcatctctat gttggaataa catgtaaagc aagatctaat tttcattctg cattaagtaa
     6301 agacaggagg atatcatgtt gtcctcctgt ctttttcatt agatactccc tactcggcta
     6361 acaactgact gagaacgtcc atttgagcct tgatcaatgc ttcggatttc gttttgaagg
     6421 tgtagacttc cgctttgagt tgttcatatc tcacctggat atttctgact ttttcggctg
     6481 cttcatctat aattttctgt gcctttattt ctgcttcatc tacaatgttc ttcgctttgt
     6541 cgcatgcatt tgccttgatt tgctcagccg tatgctgtgc aaccagaatc gagtgctgga
     6601 gcgactcttc cagtaccttg taatgctgca cggcctcatt taaagacgta atttcgcact
     6661 tcagttgatt gatactgttg ctcatctccg catagtcttc gataattttc gcaagcaggg
     6721 tgtccacctg ttttttgctg tatccgtcaa aaagcgcttt tttaagtgtt acacgtttca
     6781 ggtcagcaga tgtgtaattc acacgaccat cccctataca aactcctgta acaattctat
     6841 tcgcaaccct gcttggacaa ttcatttttt cgaaggctta attccgccca ccatcaccgg
     6901 tgccccaatc ggagatatgt ccctgccttt tacctggatt tggaaccgcg aaaaatcacg
     6961 tgtcgtgatc atatcacaaa catccctgta ccacatgaaa ctgctgatat aggcataaaa
     7021 cctggctcta tccatacact cggccgtgat cggataatac ctgcttatcc gtcgtacgaa
     7081 cccctctccc agatgatcca ggatcattgc aatatcataa gccggatcgc caaatcccgc
     7141 attgctgaat ccaataatgc tgctgattcc caaggcagca ttgttaacca gtatatgaca
     7201 cggtaccagt tctccatgta tcagtacagg ctgaaaattg aaaaattctt cgttatcaac
     7261 tgctggctgg gttatctgcc ggaaatactc ctttacatag ccagtgcaat acgggaagat
     7321 cttcctttcc aacgtttcaa tctccgccag ccattcttcc cgtccgaggt tcatccgtga
     7381 atccccgatc cgggtatttt ttgccttttt taccggaatg gaatgcaact gctccaggaa
     7441 aaccgcaatt tctctggcaa cagtatcctg cgtggatttg tcagatttca ataatacgtt
     7501 actgtacagc ggcgatcctt ttatatattc acgtttcgtt acgttctggt caactatttc
     7561 cacatccggc aaaggcaatg cgataaagtt ccggatgaat ccgagcactt ctgcttcatt
     7621 gtgcaaatag acagcactcc agtcgtattt tg
//
LOCUS       DAOSCC010000113         7544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6314_length_7544_cov_4.225798, whole genome shotgun sequence.
ACCESSION   DAOSCC010000113 DAOSCC010000000
VERSION     DAOSCC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6314_length_7544_cov_4.225798"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1536)
                     /locus_tag="PLG72_07130"
     CDS             complement(343..1536)
                     /locus_tag="PLG72_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOJ80606.1"
                     /translation="MASIGIITEKCCGCGLCLKACPFGAIRIIDKKAVIQDNCTLCGA
                     CASSCRFEAVGFIKDEAGTGADLSAYKGVWVFAEQKDGEIAGVALELLGEGRKLADRL
                     GEKLSAVLLGDGMEAAVRKLISYGADNVYVVDHPSLKKFNDESYADIFTQLVNRYKPE
                     IVLMGATTYGRSLGPRVASRLNTGLTADCTKLEIDPERRLLLQTRPAFGGNLMATIIC
                     PNHRPQMSTVRPKVMKAAEPDPSRIGEIIRPDVNIPGDVRVKVIETVSTLCEKVSLAE
                     ADIVVSGGRGLGDPKNFALVEELADVLGAAVGASRAVVDAGWVDYSHQVGQTGKTVGP
                     RIYFACGISGAVQHLAGMSSSDTIIAINKDPEAPIFKVATFGIKGDVVEVLKALIAEF
                     RKRLG"
     gene            complement(1580..2377)
                     /locus_tag="PLG72_07135"
     CDS             complement(1580..2377)
                     /locus_tag="PLG72_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ80607.1"
                     /translation="MNIVVCVKQVPGTTEVKMNKETNTIIREGVESIINPFDMHAVEE
                     SLRIKERLGGKVTVLSMGIPSVAELLKTTIGLGVDDAVLLSDRAFAGADTLATSYALS
                     MGIRKIGNVDLVICGKQATDGDTAQVGPSLAEKLGWPHTTYVRKIEEINEKFIRCQRM
                     TDDGYEVIEMPLPAVITVVKEINEPRLPSLKNMMRAKKAVVNVWTADDIGADKNLCGL
                     KGSPTQVVQTFVPVHEVRSEMIEGNAEEQARKLADKLLSMQFPAGRR"
     gene            complement(2469..3623)
                     /locus_tag="PLG72_07140"
     CDS             complement(2469..3623)
                     /locus_tag="PLG72_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOJ80608.1"
                     /translation="MDYFLTEEQQMIKELAARIADEKIAPVAIQYDEEGRFPRDIVKV
                     LADSDLCGVYIPEEYGGLGGGVFEMALVVEELSRACGGIALAFAGTGLGTYPIILFGN
                     DEQKQKYLPDIAAGRKLAAFGLTEADAGSDAAGIRTTAVRDGDEYVLNGTKQWITNGE
                     EADVFTIIACTDRNKGARGFSAFIVEKGTPGFSFGKKENKMGIRASCTSELIFENCRI
                     PKENLLAREGMGFIVAMKTLDRTRPGVAAQALGIAQGAFDEALKYSKQRVQFGQPISS
                     FQAVQHMLADMATQLEAARALVYQTCRYIDSGAKDFSKESAMCKVFASDMAMKVTTDA
                     VQILGGYGYMKEYPVEKRMRDAKITQIYEGTNQIQRNVIALELIKGLSKK"
     gene            complement(3907..4809)
                     /locus_tag="PLG72_07145"
     CDS             complement(3907..4809)
                     /locus_tag="PLG72_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ80609.1"
                     /translation="MKRFFKTIACICLLSAFALLLAGCIDIEMKVNNDGSCDLKYEIR
                     TEGMVSLSDVREQLESSIEDMNSGAGKKVAKLKDIKEKDGRIIANISVSNVSYLAGDA
                     WFGKYPDFVKEYPDYVERLYDAKSGEPVSADKIRGAGGLNVVKVDGMDTGGGDLSKFT
                     LILPGEVKYMTSNVTMIDSKTVTVGGGYGVVLYSRGGGAGWLVCVLVIAAIAVVVVVL
                     AGKKKKGAPSVSFTAAPGAAPVHSGAAAPNTANTAQEGAAAPTAAATTASAGGADIIY
                     CPQCGSQLKRGARFCSKCGGKIPE"
     gene            5169..6497
                     /locus_tag="PLG72_07150"
     CDS             5169..6497
                     /locus_tag="PLG72_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80610.1"
                     /translation="MARKVKKVKCLICGAVFDASLDVCPVCGVGSDMFVPYEEEDEDE
                     EEKSSFRKDTDEVFVILGNGAAGISAAEEIRRRNSTCSIIMVSDEPVAGYNRPMLTKK
                     LCTLKNADEIAVHDANWYKENNVVNRLGIRAEKIDTAARTVALSDGSLLNYDKCIYAL
                     GAECFIPPIQNSDKPEVVAIRRISDVMKIRELLPRVKNTVVIGGGVLGLEAAWEMSRS
                     SHVTVLEVAEKLMPRQLDDAAGDFLAGIIRSKGIDFRLKANIRAITGDSSVTGVELED
                     GSHYPADLVIISCGVRPNVTLAKEAGIAVERGITVNERMETSVPGIYACGDCAYYNGI
                     NYALWSQAVEMGKIAGANAAGEQLEYRPDPPALTFEGMDTTLFAAGDNGRNPDARYKT
                     KEVRDMAKNTFEKYFFIDGRLAGVILIGDTSRLAELTDALREKRSYEEVK"
     gene            complement(6642..>7544)
                     /gene="gatB"
                     /locus_tag="PLG72_07155"
     CDS             complement(6642..>7544)
                     /gene="gatB"
                     /locus_tag="PLG72_07155"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOJ80611.1"
                     /translation="DISDCRMEQGSLRCDANISVRPAGQKEFGTKVELKNINSFRELQ
                     KALEKEEKRQIELYRFGEGHKIVQETRRWDSGKGRTVSMRSKEEAHDYRYFPEPDLLP
                     VIIDEAAVSAIKSSLPELPMEKYRRFTREYGLSGDDADILTGDRFLSEYFENTVKEGA
                     APKTAANWVLGDVLRMLNESGISSREIPVEPARLRRLISMVEEGKISNTAGKEVFREM
                     FAGGGTPEEIVEKRGLTQISSEDALLGLVRKVIEENPKSVADYRGGKAKAVGYLVGQV
                     MKESKGRANPAMVNELLMKELDRA"
BASE COUNT         1636 a         2170 c         1807 g         1931 t
ORIGIN      
        1 gcaataacct gcatcacggc ggcttttccg gtgtccgccg ggcttttgcc tcccgtactg
       61 ctgcctgctg ccagcgcaaa cgctgatgga ccgttaagat atatcagtat tgccaacaca
      121 agtataaagc cgactttgac cctgctcaca aaatactccc cctgaaacaa gacagatgga
      181 acgacctccc ggcacacacc ggaaaaaggg accatgaaat ctatatacta atgtccatac
      241 tccagtatat ataaatttat agaatattca tagtgcctgg gcaaattttt aaaacataac
      301 aaaaaaatgc tgaaggcatc aggacggttc ccgtccctgt ttttatccca gtctcttcct
      361 gaactcggct atcaaagcct tcagcacttc tacgacgtcg ccttttatac cgaatgtagc
      421 gaccttgaag attggggctt caggatcctt gtttatagca atgatcgtat cagatgacga
      481 cattccggcg agatgctgca ctgcgccgga tatgccgcag gcaaagtata tcctgggtcc
      541 tacggtcttg ccggtctgtc ccacctggtg gctgtaatcc acccagccgg cgtcaacgac
      601 ggcgcgcgac gctccgactg cagcgccgag cacatccgcc agttcttcca ccagtgcgaa
      661 attcttcgga tccccaagtc ccctgcctcc cgatacgacg atgtcggctt cggcaaggct
      721 cactttttca caaagggtcg atacagtttc gatcactttg acccttacat ccccgggtat
      781 gtttacatca ggcctgatga tctcgccgat gcgggaggga tcgggctctg ccgccttcat
      841 caccttcggc ctcaccgtcg acatctgcgg cctgtggttc gggcatatga tggtcgccat
      901 caggttcccg ccaaaagccg gacgcgtctg cagcagcagc cgcctttcag ggtcgatctc
      961 cagtttcgta caatcggcgg taagccccgt gttcagcctc gatgcgaccc tcggccctag
     1021 cgagcggccg taggtcgtcg ctcccatgag cacaatttcc ggtttgtacc tgttgaccaa
     1081 ctgcgtgaat atatcggcat atgattcatc attgaacttc ttcagtgacg ggtggtcgac
     1141 cacatataca ttgtcggcgc cgtaggatat gagcttgcgg actgccgcct ccatcccgtc
     1201 gcccagcagc accgccgaaa gcttttctcc aagccggtcc gccagtttcc ttccctcgcc
     1261 gagcagttcc agcgctactc cggcaatctc tccgtccttc tgctcggcaa atacccatac
     1321 gcctttatat gcggacaggt ctgcgcctgt tccggcttca tcctttatga acccgacagc
     1381 ttcgaacctg catgatgagg cgcaggcgcc gcacaacgtg cagttgtcct gtatcacagc
     1441 ttttttatcg attatccgga tggcgccgaa aggacaagct ttcaggcata acccgcatcc
     1501 gcagcacttt tccgttatta tcccaatact ggccatcgaa aaaaccccca agcatgttat
     1561 caatattgaa cgatctgcat cagcgccttc ctgccgggaa ctgcatcgac aggagtttgt
     1621 ccgccagctt cctcgcctgt tcctctgcat tcccctctat catttcgctt ctgacttcat
     1681 ggaccggcac gaatgtttga actacctgcg tcggggaacc tttcagcccg caaaggtttt
     1741 tgtcggcgcc gatgtcatcc gccgtccaga cattcaccac ggcctttttg gctcgcatca
     1801 tgttcttcag cgacggcagc ctcggttcgt ttatttcctt taccacggta atgactgccg
     1861 gaagaggcat ttctatgact tcgtagccgt cgtcggtcat cctctggcac ctgatgaact
     1921 tctcgtttat ctcttctatt ttccttacgt aagtggtatg gggccagcca agcttttcag
     1981 caaggctcgg tcccacctgc gcggtatctc cgtcggttgc ctgcttgccg cagatcacca
     2041 ggtccacgtt ccctatcttc cttatgccca tcgacagcgc atatgaagtc gccagcgtgt
     2101 cagcgccggc aaatgccctg tcgctcagca gcacagcatc gtccactcca aggccgatgg
     2161 ttgttttgag aagttcagcg actgacggga tgcccatgct cagtactgtg acctttccgc
     2221 caagccgttc ttttatcctc aggctttcct ccaccgcatg catgtcaaac gggttgatta
     2281 ttgactcgac cccttcgcgg atgatggtat tggtctcctt gttcattttt acctcagttg
     2341 tgcccgggac ctgttttaca catacgacta tattcattcg atcatacact cctgacgctt
     2401 gatttgtcgc cgacttatcc ggatgtgttt ttatagcggc gccctgctga cagtccggca
     2461 tcggtaggct attttttcga caatcccttg atcagttcca gcgctatgac atttctctgg
     2521 atctggttcg tcccttcgta tatctgggtg atcttggcgt cacgcatcct cttctccacc
     2581 gggtattctt tcatataccc gtaaccgccg aggatctgca cggcatctgt cgtcaccttc
     2641 attgccatat ctgatgcgaa taccttgcac atggcggatt ccttcgagaa atccttcgca
     2701 ccgctgtcga tgtagcggca tgtctggtag acgagcgccc ttgccgcctc aagctgtgtt
     2761 gccatatcgg caagcatgtg ctggacagcc tggaacgacg atatgggctg gccgaactgc
     2821 actctctgct tcgaatactt aagcgcttca tcgaacgctc cctgtgcgat gccgagagcc
     2881 tgtgcagcca cacctggcct ggtcctgtcg agggtcttca tcgctacgat aaagcccatt
     2941 ccctccctgg caagcaggtt ttccttaggt atcctgcagt tttcgaatat gagctcgctg
     3001 gtgcatgaag cccttatgcc catcttgttt tccttttttc cgaaggagaa ccccggggtg
     3061 cctttctcga cgatgaatgc cgagaatccc ctggcgccct tgttcctgtc ggtacatgct
     3121 atgatggtga acacgtcggc ttcttccccg ttcgtgatcc attgcttcgt tccgttgaga
     3181 acatattcat ccccgtcacg cacggcagtt gtcctgatgc cggcagcgtc acttccggcg
     3241 tcggcctcgg tcagtccgaa tgccgcaagc ttccttcctg ccgctatgtc gggaaggtat
     3301 ttttgcttct gttcatcgtt tccaaaaagg attataggat atgtaccaag cccggtcccc
     3361 gcaaatgcca gcgcaatgcc gccgcaggcc ctgctcagtt cctccaccac cagcgccatt
     3421 tcgaaaacgc cgccgccgag cccgccgtat tcttccggta tgtaaacgcc gcacagatcg
     3481 gaatctgcaa gcaccttgac tatatcacgc ggaaaccttc cttcctcgtc atactggatg
     3541 gcgaccggag cgatcttctc atcggctatc ctggcagcca gttcctttat catctgttgt
     3601 tcctcggtca ggaaataatc cataaggcaa aacacactcc ttttcgcgca gggctgatgc
     3661 agtcagcctt aagtccgtcc gcaggccagc ccggcgccgg cattatttta gtatttgata
     3721 atatcatctg gtaataatat cagatattta gagtataata caaattacca tcctgtgcaa
     3781 gagcagccgg tattgaaaat tccatacctt ttacttgggc agaacaacag ggccaggcaa
     3841 aaggatgaac cctcgaacct ggctccggta catttcaata gtagatctgt cgtgcagtac
     3901 tcctgtttat tccggtattt tcccgccgca ttttgaacag aatctggcgc ctctcttcag
     3961 ttgtgaaccg cattgcgggc aatatatgat atcggcgccg cctgcggaag ccgtcgttgc
     4021 tgccgctgtt ggtgctgccg ctccttcctg ggctgtgttt gcagtattcg gggccgcagc
     4081 gcccgaatgt accggcgctg ctccaggagc cgccgtaaat gatacggatg gcgcgccctt
     4141 tttcttttta cctgccaaca cgaccactac aactgcgata gctgcgatca ccagcacaca
     4201 gaccagccat ccggccccgc cgcctctgct gtacaggact acgccgtagc ctccgccgac
     4261 agtgacggtt ttgctgtcga tcatcgtcac atttgatgtc atatacttca cttcacccgg
     4321 aagtattagt gtgaactttg acaaatcacc gccgccggtg tccatgccgt ccacctttac
     4381 cacgttgagt cctccggcgc ccctgatttt gtccgcgctt accggttcgc ccgatttggc
     4441 atcatacagc ctctccacat aatcagggta ttcctttacg aaatccggat actttccgaa
     4501 ccaggcatcg ccggctaagt aggaaacgtt gctgacactg atatttgcga tgatcctgcc
     4561 gtctttctcc tttatgtctt tcagcttcgc aacctttttg cctgcaccgc tgttcatatc
     4621 ttcaatactg ctctcaagct gttccctgac atcagaaagg ctgaccatgc cctccgtcct
     4681 gatctcgtat ttcagatcac atgagccgtc attgttgacc ttcatctcga tgtctatgca
     4741 tccggccagg agcagtgcaa acgctgacaa gagacagatg catgcaatag ttttgaaaaa
     4801 ccttttcata acagacccct tccatcatga catatcgttt ccgcatatgc gccgccaatg
     4861 agacgggtga catataccgc ctcattatat agaaagctat tcgacgcctg tttccaattt
     4921 ccttttatcg attacaaata ttactcttta tccggaagta tcttctcatc ggcaggtcac
     4981 gggcttatac cgggtctgcg ctgcgcgatc cgcttcccca ggccggtccg ggtttatgcc
     5041 gggatcgact atgccgggac cgtcctgatc atatctctgt accaatttct gtttgaaaag
     5101 ttgggatcca ggtatagaat aaaagtagtg cattttcatt aacagtattt acaatggagg
     5161 aatattaaat ggcgaggaaa gtaaaaaagg taaaatgcct catatgcggc gcggtattcg
     5221 atgcatccct cgacgtatgc cctgtttgcg gcgttgggtc ggatatgttc gtaccgtatg
     5281 aagaggagga tgaggatgag gaagagaaat cttcgttcag gaaagatacg gacgaggttt
     5341 tcgtgatcct gggcaacggc gctgcaggca tcagcgcagc agaagaaata aggaggagga
     5401 attccacatg ttccataata atggtgtccg acgaacctgt ggcaggctac aaccgtccaa
     5461 tgctgaccaa aaaactgtgc acgctgaaaa atgccgatga gatcgctgtc cacgacgcaa
     5521 actggtataa agaaaacaac gtagtaaaca ggctcggcat acgcgcagag aaaatagaca
     5581 ctgctgccag gacggtggcg ctcagcgacg gcagcctctt aaattatgat aaatgcatat
     5641 acgctctcgg cgccgaatgt ttcataccgc cgatacagaa cagcgacaag ccggaagttg
     5701 tggcaatacg ccggatatcc gacgtgatga aaataaggga gctcctgccg agggtcaaaa
     5761 atacagtcgt cataggggga ggcgtgcttg gtctcgaggc tgcatgggaa atgagcagat
     5821 caagccatgt tacggtcctc gaagtggctg aaaaactcat gccgcgccag cttgacgatg
     5881 ccgcgggaga cttccttgcc ggaataataa gaagcaaagg catagatttc aggctgaagg
     5941 caaacatcag ggcaataaca ggcgacagtt ctgtgacagg cgtcgaactt gaagacggct
     6001 cgcattaccc ggccgacctg gtaataatat catgcggagt gcgtccgaat gtcaccttgg
     6061 ccaaagaggc cggcattgca gttgaacgcg gcattacagt caatgaaagg atggaaacca
     6121 gcgtaccggg gatctatgcc tgcggagact gtgcttatta caacgggatc aactacgccc
     6181 tctggtccca ggcagtggag atgggaaaga tcgcaggcgc caatgctgcc ggtgagcagc
     6241 ttgaatacag gcccgatccg cctgccctta ccttcgaggg catggatacc accctgtttg
     6301 ccgcaggcga taacggaagg aacccggatg ccaggtataa gacaaaagaa gtcagggata
     6361 tggcgaaaaa cacttttgag aaatacttct tcattgacgg caggctggca ggcgtcatcc
     6421 ttataggcga tacatcaagg cttgctgaac tgacagatgc gctcagagaa aagcgcagct
     6481 atgaggaagt taagtaaggc tgcaggtaat acgaagccat gaaacggtat cttgaagctg
     6541 agtgaaaaat ggccgggtcc gaatgtaatg attcggaccc ggttttgatt ggatccctgt
     6601 ccggccatgg cccgggcgag gtccgtttgt ccgtgatacg ctcaggccct gtcaagttct
     6661 ttcatcagca gttcgttgac cattgcgggg ttcgccctgc ccttcgattc cttcattacc
     6721 tggcctacaa gatatcccac ggctttcgct tttcctcccc ggtaatccgc cactgatttc
     6781 ggattctctt cgatcacctt cctgaccagt cccaggaggg catcctcgga actgatctgg
     6841 gtcaggcctc ttttctcgac tatttcttca ggcgtcccgc cgccggcgaa catttccctg
     6901 aaaacttctt tgcccgccgt attgctgatt ttcccttctt ccaccatgga tatcagcctg
     6961 cggagccggg ccggttctac gggtatttcc cgggacgata tgccgctttc attgagcatc
     7021 ctgagcacat cgccaagcac ccagttcgca gccgttttcg gcgcagcgcc ttctttgacc
     7081 gtattttcga aatactccga caaaaatctg tcgcctgtga ggatatcggc gtcatcgccg
     7141 ctcagcccgt attcccgagt gaaccttctg tatttttcca tcggcagttc gggaagcgag
     7201 ctttttatcg ccgaaacagc ggcttcatcg atgatgaccg gcaaaaggtc aggttcgggg
     7261 aagtacctgt aatcatgcgc ctcttccttg gacctcatcg atacggtcct tcccttgccg
     7321 ctgtcccatc tcctcgtttc ctgcactatc ttgtggccct cgccgaaccg gtacagttcg
     7381 atctgcctct tctcttcctt ctcaagcgct ttttgcagtt ccctgaagga gttgatgttc
     7441 ttcagttcga cctttgtacc gaattccttt tgacctgcgg gcctcacgga gatgttcgcg
     7501 tcgcacctca gcgaaccctg ttccatgcgg cagtcggata tatc
//
LOCUS       DAOSCC010000114         7533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6329_length_7533_cov_4.673442, whole genome shotgun sequence.
ACCESSION   DAOSCC010000114 DAOSCC010000000
VERSION     DAOSCC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6329_length_7533_cov_4.673442"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1942)
                     /locus_tag="PLG72_07160"
     CDS             complement(194..1942)
                     /locus_tag="PLG72_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ80612.1"
                     /translation="MKKKGMALLAALVCAALALTMTACGSKKGVQDANTALSGNTSGT
                     TSSADTAPSASSPGLPQYAPKPGKINIQFSESAAKDGMMPAGTEAGAVAIASMEQYGM
                     PFEKAQPETQFNTEEYKFVEENRMMKVSMSPLSTFSIDVDTASYSNVRRFLGNGMKPP
                     ADAVRIEEMVNYFAYDLPGPDGDVPFSVTTELARCPWNKNNYLAMIAIQGKKVEHGNL
                     PKSNLVFLLDVSGSMDEPMKLPLLKSSFQLLAEQLGENDRVSIVVYAGASGTVLEGAR
                     GDEKEKILAALDSLEAGGSTAGAAGIELAYKVAKKYFINGGNNRVILATDGDFNVGVS
                     SESELARLIEKKREEGIYLSVLGFGYGNYKDNKLEALADKGNGNYAYIDSLMEAKKVL
                     VNEMGSTLLTIAKDVKIQVEFNPAKVKEYRLIGYDNRLLRNEDFDDDTKDAGEIGAGH
                     NVIAFYEIVPYGDGETPELPQLKYQAAEMRQEWMDEWMEVRIRYKQPDSDVSEKIVHT
                     ITDRQIADNPSENFMFASAVAEFGLILKNSEYKGSASLNSVKSRAEAAMGDDEEGYRR
                     NFLELVDKAKEIGIDG"
     gene            2473..3606
                     /locus_tag="PLG72_07165"
     CDS             2473..3606
                     /locus_tag="PLG72_07165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ80613.1"
                     /translation="MLRILHLLNYLGSGGSEKYIYSLAKKLHGGSCEFFIAYSNDGPG
                     RSMFEELGISLIPLGMNSPFDIKAAMKLKKLCRQLSIDVVHTHFLRENYISVLSKIAG
                     NKVGIINTRHMLFENSRMVVLSNRLFTRFNSRIIAVSGHVRDRLVREGINENRIAMIY
                     NGVDPAEWDSAAAAGFRKEMGISEEDMLITSVSRFSPEKGHDFIIDVIGYIKENMGAL
                     GLAGKKFRFVLAGSGPLQEAVIEKAKENGLQDIIIFPGYVNNIRDLLKDSDIFIAHSQ
                     NEALGIAILEAMAAGLPVITTDSGGTSEIVNCSEEDGILVDYGDVQGYAEGLALLIND
                     SHLRRQFADNGLKVVRERFSLDKTAAETYNLYELSRKGEKDDR"
     gene            3596..4084
                     /locus_tag="PLG72_07170"
     CDS             3596..4084
                     /locus_tag="PLG72_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HOJ80614.1"
                     /translation="MIDKNGKLFGRINIIDLSFLVILLAAALFILHAAGVFSPEKAAA
                     PEEKMLLTMFQEEVNSFTPSNVKTGDPVTESFQNISFGKLVDIETGDSINWGADEEGR
                     QVKTKKDGFVSVRLVMETPGKVGPSGLTIGGAKYYIGQFYVIRVGKATFYARIESAEQ
                     IR"
     gene            4103..4579
                     /locus_tag="PLG72_07175"
     CDS             4103..4579
                     /locus_tag="PLG72_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HOJ80615.1"
                     /translation="MLKKRSFWIIIAVIVIVAAGIAGMSDKFAKAKVGAPAAAKDKII
                     ITFYMENTPDCTIDAIKAGDPVREQIQNSSFGKVTAVERGDSIFWANKDSGEFVASPR
                     EGYSSLTLTMEAYGTITGNGVSIDKSVYYVGQTISLYAGNSYLKDGRISGIEKAAD"
     gene            4603..6048
                     /locus_tag="PLG72_07180"
     CDS             4603..6048
                     /locus_tag="PLG72_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOJ80616.1"
                     /translation="MKEFIKNTIDSSLVISYFRGQDKSQQSWAESRVLRIIHLIVNFP
                     GKLLKSAYKKWENVFESSIAFRLLAYLAYNMHIVIGLALVLLMAVPHHIWRNGYSVVL
                     AVGLAALLLIRLMKNRESVIETPAIDYSIWLFFVSLLVAAATSLFPVDSLKYLVHYIV
                     AFVFIAVIVSAIDSARDLNMLIRLIAFGTFLTALYGIYQWKVTGIAVDPSTTDLTLNP
                     DLGGRVYSTMGNANVYGELLVLTIPFFAAVILNEKNLLKKAAWLAMLAPVLLVLFKTG
                     SRGAWVSLAGAAVVFVFCWNIKFIPVIIVAGLIALQLLPAPIYNRIMTIFSGDKSTDY
                     RGMILTSAKPMLKDYWLTGVGLGPGVVSTIFERYKIFGLTKAAHTHVLYLQLWLEAGL
                     AGILTFLLMIFRMVRSVFEVIINRKDPAANNVMFAAISGIAGLLAMGLADHVWFFQRI
                     MLLFWVNIAIILTVVKVTNRNRESIEKDGID"
     gene            complement(6336..6782)
                     /locus_tag="PLG72_07185"
     CDS             complement(6336..6782)
                     /locus_tag="PLG72_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ80617.1"
                     /translation="MFGLVPFNRRNSLASGKDFFSIDNFFDNFFNDSFAGFFTAVNPI
                     RADIRETEKEYIIEADLPGVRKEDIRLELEDGVLTLGVEQNEQINEERENYIRRERRY
                     GSYCRSFRVDGVREDKVTAKYDNGVLTVKLPKSEESKPKSHRIDIN"
     gene            complement(7060..>7533)
                     /locus_tag="PLG72_07190"
     CDS             complement(7060..>7533)
                     /locus_tag="PLG72_07190"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOJ80618.1"
                     /translation="CIFIYLQQTLTGVLNGLGKQGILLRNTVIGSIIRIAVICVLLPV
                     FGIKAYIIGLTVSLLLTECMDLIEINKMTGLVIDLREWIVKPALAGVLMIVAGRYIYH
                     FFEIFSFGARLTTLLSVAVDVMFAVVLLMLVGVFELRELLSMAGLDNMMKTGKKM"
BASE COUNT         1782 a         1909 c         1910 g         1932 t
ORIGIN      
        1 tgtttcaacg agacgaagtc tcgttataac acctaacggt atcaaatccg ccggcaatga
       61 aaaagtacca ggcggttgga cataaccgca aggtactttt tataataaca tctattgcct
      121 tatcctgctg tataacttct attgccttcc tccaccgtaa tccatttttc ggccttatcg
      181 cctcatcccg gcatcatccg tctattccta tctcttttgc cttatcgaca agctcaagga
      241 agttgcgcct gtagccttcc tcatcgtcgc ccattgcagc ttcggcgcgg ctcttcacgc
      301 tgtttaggga cgcgctgccc ttgtactccg agtttttcag tatcagcccg aattcagcca
      361 ctgccgaagc aaacatgaag ttctcggacg ggttgtctgc aatttgccgg tctgtgatgg
      421 tgtgtacgat tttctcgctc acgtcggagt caggctgctt gtagcggatc cttacctcca
      481 tccactcatc catccactcc tgccgcatct cggcagcctg gtacttaagc tgcggcagtt
      541 ccggagtctc accgtcgccg tacggaacga tctcatagaa ggctatgaca ttgtggccgg
      601 ccccgatctc gcccgcatcc ttcgtatcgt cgtcgaagtc ctcattgcgc aggagcctgt
      661 tgtcatagcc gatcaggcgg tattccttca cctttgccgg gttgaattcc acctgtatct
      721 tgacgtcctt tgcgatggtc aggagagtgg aacccatttc gttgaccagc accttctttg
      781 cctccatcag gctgtcgatg tatgcgtaat tgccattgcc cttgtcagcc agcgcttcca
      841 gtttgttgtc cttgtaattc ccgtagccga agccaaggac actgaggtaa atgccttctt
      901 ccctcttttt ctcgatcagc ctggcaagtt cgctctcgct gctgacgcca acgttgaaat
      961 caccgtcggt ggcaagtatg acacggttgt tgccgccgtt gataaaatat ttcttcgcga
     1021 ctttgtaggc gagttcgata ccggctgcgc ctgcggtgga acctcccgcc tcgaggctgt
     1081 ccagggcggc aagtatcttt tctttctcat cgccgcgggc tccttcgagt acggtaccgg
     1141 aagcaccggc atacaccact atggagaccc tgtcattttc gccaagctgc tctgccagca
     1201 gttggaacga ggatttcagc aggggcagct tcatcggttc atccatggag cccgacacat
     1261 ccagcaggaa tacgagattt gatttgggca ggttaccatg ctccaccttt ttgccctgga
     1321 tggctatcat tgcaaggtag ttgtttttgt tccaggggca ccgggccagt tccgtcgtga
     1381 ccgagaaagg cacatcgccg tccggcccgg gcaggtcata cgcaaaatag ttgaccattt
     1441 cctcgatcct taccgcgtct gcaggcggct tcatcccgtt gccgaggaat cgtcttacgt
     1501 tgctgtatga agctgtgtct acatcgatgg aaaaggtgga cagaggcgac atgcttactt
     1561 tcatcatgcg gttctcttcg acaaacttgt actcttccgt attgaactgt gtctcaggct
     1621 gcgctttttc gaagggcatt ccgtattgtt ccatagacgc tatggctacg gctccggctt
     1681 ccgtaccagc aggcatcatg ccgtctttcg ctgccgattc actgaactgg atgttgatct
     1741 tgcccggctt cggagcgtat tgcggcaagc ctggggaaga agccgacggt gccgtatccg
     1801 cggaagaagt cgtcccggat gtattcccgg aaagcgctgt gtttgcatcc tggaccccct
     1861 ttttagagcc gcacgcggtc atggtcagtg ccaaagctgc gcacaccaat gctgccagta
     1921 aggccattcc tttctttttc atttttgttc ctccttttat cttatttatc tctgtctaat
     1981 agattagatt ggataaacag cataaaagtt ccagttgtca agtgctttgc gtgcctgtac
     2041 gtttcgctgg tttcagcctg cacacactgg actccggggg tcccggaaaa tgacttcatg
     2101 gtttccggca gtaaaatacg gcaagcgtgt gctgcctgac caaattaaac gcctcactgt
     2161 cctgaccgga ataaatgcct catacactcg actcgcggga acgtatgtga tagaatataa
     2221 cgaggcgata aaatacccga cctttataga tggcgggtat tgacttgcct ttcaggcgaa
     2281 gagtatgtct cccgcctcgt tgaaacgaac aaagataaga aaaatttcta taaactataa
     2341 tcgaaaattt ttcttggatg cgttataatg cagttggtta agcgagccta tgcgacaggt
     2401 tccggtcact tcgtgactta gttgtttcgg ttattacaga ggataaagtc gtcctcggca
     2461 gggcggtgtt atatgctcag gatattgcat ttgctgaact acctcggaag cggaggatct
     2521 gaaaaataca tatattcact ggctaaaaag ctgcatggcg gatcatgcga gttttttatc
     2581 gcatactcca atgacgggcc gggaagaagc atgttcgagg agcttgggat cagcctgatc
     2641 cctcttggga tgaacagccc tttcgacatc aaagcggcca tgaagttgaa aaaactttgc
     2701 agacagcttt cgatcgacgt cgtccacaca catttcctga gagagaatta tatcagcgta
     2761 ttgtccaaaa tagcagggaa caaggtcggc atcataaata cgaggcatat gctgtttgag
     2821 aactccagga tggtcgtgct gtccaacagg ctcttcacca ggttcaacag ccgcatcatc
     2881 gccgtcagcg ggcatgtcag ggacaggctc gtcagggagg gcataaacga aaacaggata
     2941 gcgatgatat ataacggggt ggatcccgcc gaatgggaca gcgcggctgc agcaggtttc
     3001 agaaaggaaa tggggatatc cgaggaggac atgctgatca cctcggtttc aaggttttca
     3061 ccggaaaaag gccatgattt catcatcgac gttatcggtt acataaaaga aaacatgggc
     3121 gccctcggcc ttgcggggaa aaagttcagg ttcgtgcttg caggcagcgg cccgctgcag
     3181 gaagcagtca ttgagaaggc caaagaaaac ggactccagg acatcatcat tttcccgggc
     3241 tacgtgaaca acatcaggga tttgctcaag gattccgata tatttatagc gcattcacag
     3301 aacgaggctc tcggtatagc catactcgaa gcgatggcag ccgggctgcc ggtaataacg
     3361 actgacagcg gcggcacttc cgagatcgta aattgcagcg aggaagacgg gatcctcgtg
     3421 gactacgggg atgtgcaggg ctatgcggaa ggacttgccc tgctgataaa tgacagccac
     3481 ctcaggcggc agtttgccga caacgggctg aaggtggtca gggagcggtt cagccttgat
     3541 aaaaccgctg ccgaaacgta taatctttat gaactgagca ggaagggaga aaaggatgat
     3601 cgataaaaac ggaaaactgt tcggcaggat aaatatcata gacctttctt tcctcgtgat
     3661 actgctggct gcggccctgt tcatcctgca cgccgcaggg gtgttttcgc ctgaaaaggc
     3721 ggcggctccg gaagagaaaa tgctgcttac aatgttccag gaagaagtca actcatttac
     3781 gccatcgaac gtcaaaacag gcgatcctgt gacggagtcg ttccagaaca tcagcttcgg
     3841 caaacttgtt gatatcgaaa ccggcgattc catcaactgg ggagcggacg aagaaggcag
     3901 acaggttaag acgaaaaaag acggattcgt ctctgtcaga ttggtaatgg aaacgccggg
     3961 aaaggtcgga ccttccggac ttaccatagg aggggcgaaa tactatatcg gccagttcta
     4021 tgtgatcagg gtcggcaaag cgactttcta tgcgaggata gagagcgcgg agcaaatcag
     4081 gtgataaggt gggatggata cgatgttgaa aaaaagatcc ttctggataa tcatagcggt
     4141 cattgtgatc gtggcggccg gcatcgcggg gatgtccgac aaattcgcaa aggctaaggt
     4201 aggcgcgcct gctgcggcca aagataagat aatcattact ttttatatgg aaaacacgcc
     4261 cgactgtacc attgacgcca taaaggccgg cgacccggta agagagcaga tccagaattc
     4321 cagcttcggc aaggttacgg cagttgaacg gggcgactcc atcttctggg caaacaagga
     4381 ctcgggtgaa ttcgttgcct cgccgaggga aggctactct tcgcttacgc tgaccatgga
     4441 ggcatacggc accatcaccg gcaacggcgt ttccattgac aagtcggtat attacgtagg
     4501 ccagacgata tcgctttacg caggcaacag ctacctcaag gacggaagga tatcaggcat
     4561 agaaaaagcc gctgactaag ctgctgattg aggatggtca caatgaagga gtttattaaa
     4621 aatacaatag atagcagcct ggtcatctcg tatttcaggg ggcaggacaa atcccagcag
     4681 agctgggccg aaagccgtgt cctccgcatc atccatctta tcgtcaattt cccgggaaag
     4741 ctgctgaaat ccgcctacaa aaagtgggaa aacgtgtttg agagcagcat cgctttcaga
     4801 ctgctggcgt acctggctta caacatgcac atagtcatcg ggctggccct ggtactgctc
     4861 atggccgtac cgcaccacat ctggcggaac gggtacagcg ttgtcctggc agtcggcctg
     4921 gctgcgcttt tgctgatcag gctgatgaaa aaccgggaga gtgttataga aacgcctgcc
     4981 atcgactatt cgatatggct gtttttcgta agcttgcttg tggctgccgc aacgtcgctg
     5041 tttcccgtcg acagcctgaa atatctcgtc cattatatcg ttgcgttcgt attcatagca
     5101 gttatcgtca gcgccatcga ttcggccagg gacttgaaca tgcttatcag gctcatcgct
     5161 ttcggcacgt tcctgactgc gctttacggc atatatcaat ggaaagtgac cggaatagcc
     5221 gtcgacccgt cgacgaccga ccttacgctg aatcccgacc tgggcggcag agtctattcc
     5281 accatgggaa acgccaatgt gtacggtgaa ctgctggtcc tcacgatacc gtttttcgcg
     5341 gcggtaatac tcaatgaaaa aaacctgctt aaaaaggcgg catggctggc catgcttgcg
     5401 ccagtgctcc tcgtactgtt caagacaggc tccaggggcg cgtgggtctc cctggcaggc
     5461 gcagccgtgg tattcgtttt ctgctggaat attaagttca taccggtgat aatcgttgcg
     5521 ggccttatag ccttgcagct gctgcctgcg ccgatataca acaggataat gaccattttc
     5581 agcggcgata aatccactga ttacagggga atgatactca cctcggcaaa acccatgctg
     5641 aaggactact ggctgacagg cgtcgggctc ggcccgggcg ttgtttcgac gatctttgaa
     5701 agatacaaga tattcggcct caccaaggcc gcccataccc atgtgctgta cctgcagctc
     5761 tggctggagg caggtctggc cggcatcctg actttcctgc tgatgatatt caggatggtg
     5821 cgcagcgtat tcgaagtgat aataaacagg aaggaccctg ccgcgaataa cgtgatgttc
     5881 gctgcgattt ccggcatcgc cggccttctt gccatgggcc tcgccgacca cgtgtggttc
     5941 ttccagcgga taatgctgct gttctgggta aacatagcca taatacttac agtcgtcaaa
     6001 gtaacgaaca ggaacaggga aagtattgag aaggacggca ttgattagca cccgggtcca
     6061 ttggttccgg gctgcagaaa gtcatgcagg caatcctgct tctgtacggc agcagacata
     6121 cttcggaacg tcgtttatca agcagaaaaa cagccggtat atgtgggggg aaacagcata
     6181 caccggcttt ttatcgtaat caggtaccaa tttctttttt atgagatgta atggatgaaa
     6241 gttccgaccg gccggcggcc tgatccaacg acggatcgca gccggccggc tgctcagttg
     6301 ctgtcggtca gcagttgtcg gtcaatcgct gtcgatcagt ttatgtcgat cctgtgggac
     6361 tttggctttg attcctcgga cttgggaagc ttgacagtga gtacgccgtt atcgtatttt
     6421 gcggtcacct tgtcttccct gaccccgtcg accctgaagc tcctgcagta cgaaccgtac
     6481 cttctttccc tgcggatgta gttttctctt tcctcgttga tctgttcgtt ctgctctaca
     6541 ccaagggtaa ggaccccgtc ctcaagctcg agcctgatgt cctctttcct tacgcctggt
     6601 aagtccgcct ctatgatgta ttccttttcg gtctccctga tgtcggccct gatcgggttc
     6661 actgccgtga agaatcctgc aaacgaatca ttgaagaagt tgtcgaagaa gttgtcaatg
     6721 ctgaagaaat cctttcctga cgctaagctg ttccttctgt tgaacggtac cagtccgaac
     6781 atataaccac ctccgaaaaa tttatcaaac ttttttagtg cattttatga attgtttaca
     6841 taaaatgctg cattttcttt gctttccgct gccttgtctt ttgtatgtag tttattttgt
     6901 ctttagttgt cttgttttgt ctttgactat ctttgacctt cgttacgatt ttattttaat
     6961 ctataaacat gtgaaatgca aatttgattt tgactttctt tgacctattg atatggaaat
     7021 gcctgtatat tttgaaatag ctcacgattt ctaactcatt cacatttttt tgcctgtttt
     7081 catcatatta tcaagtccgg ccatactcag caattcccgg agttcaaaca ccccgaccag
     7141 catcagcagc acgaccgcga acatcacatc aacagccacg gagagcagcg tcgtaagcct
     7201 ggcgccaaag gagaatatct cgaaaaagtg gtagatatat ctgcctgcca cgatcatgag
     7261 aacacctgcc agggcgggtt ttacaatcca ctccctcaaa tcgatgacaa ggcctgtcat
     7321 tttgtttatc tctatgaggt ccatgcattc cgtaagcagc aggctcacgg tcaggcctat
     7381 aatgtacgct tttatcccga atacgggcaa aaggacgcag atgacggcta tcctgattat
     7441 cgagcctatc accgtgttcc tcagcaggat gccctgcttc ccgagcccgt tgagaacccc
     7501 ggtcagcgtc tgctgaagat atatgaaaat gca
//
LOCUS       DAOSCC010000115         7479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6378_length_7479_cov_4.215383, whole genome shotgun sequence.
ACCESSION   DAOSCC010000115 DAOSCC010000000
VERSION     DAOSCC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_7479_cov_4.215383"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLG72_07195"
     CDS             complement(<1..1135)
                     /locus_tag="PLG72_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOJ80619.1"
                     /translation="MKIQEREFIPVLLGNDINTYGMSRAFYEAYGVRSVVIGKAATGP
                     SCNSRIIDFRHVPGLDTEEIFIKTIRKTAAEFAEKKVILMGCGDNYVEQIIRNSARLP
                     ENIVAPYVDEQLMDRLLTKTEFYRMCDSHGVEYPDTFIYERSMGSDFTLPFGFPVFLK
                     PSNGIRYWQHEFPTQKKAYRLNSEDELKEVIEQIYGAGYDDRLIIQDFVPGDDSNLRV
                     MICYSGRDGKVKLMSFAHVVLEEHTPHGIGNTAVLMNDHDAGLSEKLRNFLDSIGYTG
                     FSTFDIKYDRRDGRYKILEMNLRQGRSNYYVTAGGNNLAKYVVEDRIYNKNLQLEIAD
                     TRNLWTVIPLGVAFRYTRDENIRRQMKELVREKKVTNPLFMKGD"
     gene            1367..2689
                     /locus_tag="PLG72_07200"
     CDS             1367..2689
                     /locus_tag="PLG72_07200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOJ80620.1"
                     /translation="MPKPFKGPDMPRPFSLPEGFLLGSATAATQIEGGDRNNNWYAWC
                     EKGKIKDGTSCLRADDHWNRYIEDIELLKRLDHKVYRMGLEWSRIEPEKGKFDGAAIA
                     RYRDELQRLIQNGIRPLVTLHHFTHPLWFCAEGEFESEKSIGYFERYTRYVVENIGDL
                     VSEYITINEPNVFVTNGYFMGEWPPGKKDLGLAMKVYRNMALCHIAAYKAIHEVRREK
                     GFDRESRTMVGVANHLRVFDPYSRNPLDWLAARVMCWLFQGAIIRSMSSGRLRFPLGS
                     GAPLGTGRFYDFIGINYYTRSAVRFKGFRSDVMPGTPRNDLDWEIYPEGLYILCREFY
                     KKYRAPIWITENGTCDGNDSFRAGYIYDHLYIVSMLCGEGIPVERYYHWTLMDNFEWL
                     EGESARFGLVHVDYETQARTVRKSGRFYSEICRNNGVTEEMIREHLLE"
     gene            complement(2735..4621)
                     /locus_tag="PLG72_07205"
     CDS             complement(2735..4621)
                     /locus_tag="PLG72_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ80621.1"
                     /translation="MLLLEAVNVKKYYGDRLVIGFDELKVYSGDRIGVTGLNGSGKTT
                     LLDMLYGSLEPDEGYVRRYCEVGYIRQFGMDGKSDIRQLRTEAAGAAMKLLSEFEVGG
                     KLDRPALSGGEQTRLKIAGAFSRETVLMFADEPTSNLDYKGIELFRQKLGKVESFIMV
                     SHDRSLLDALCNRIIEVKDGGIRIFNGNYSFYREQCEAERQRAAAEYEKYIAEKTALE
                     QAISDRMRRAGKVRKAPSRMGNSEARLHKRHAAESQEKLHSAASSLITRLEKLEVKER
                     PKEQPVIRLDLSLTEPPENKIIISADRLSFSYGNVPVFKNAGFRITNGMKTALWGENG
                     TGKTTLLNLVYTAYLARSGSYGTGVPVPDQGHPDTAGRRTGIMPEEFAGTAGQVMSMH
                     TGAGTSTGADAGTGFDPYMWLADRAAAGAARCINIVPRARLAYFRQTFENLDPDKTVL
                     ENVMKDSVQNETVARTILARLLLPGANVFKKVGILSGGEKIKVSFAKLLVSRANVLLL
                     DEPTNYLDIQSIEALENVLKDYEGTVLFVSHDRAFVRAVADRLLVTGGLDVRSFEGGL
                     DEYEESIKTADGAKNMELEKTMLRMRITEVVTKLSLQPADREALEEEFRRLTKQLRDL
                     ESTN"
     gene            complement(5099..5782)
                     /locus_tag="PLG72_07210"
     CDS             complement(5099..5782)
                     /locus_tag="PLG72_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80622.1"
                     /translation="MQLLKYEDFISRVNELGFMFFSNVMDGMPSLVSETPYWAWHTGD
                     PETDPWRWKDRAAEEKRLAFGCVLGGNKGFIAPHMYAVFLAACKPSRTMRERRYEGLV
                     SQACWDLWQLFEWKKVLDTSEIRSHMGVRGKKSGSSKVDSAIVELQKQFYITVGGSRQ
                     KIDRNGRPYGWHINVYERVEDWAPAQWCGGKQKMDREDAVDLILDTGMSIGKNVDRDK
                     LAKVLGFSL"
     gene            complement(5971..>7479)
                     /locus_tag="PLG72_07215"
     CDS             complement(5971..>7479)
                     /locus_tag="PLG72_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOJ80623.1"
                     /translation="ERMVTGVLKETLVNRLYARSEPDDPAGSEEPGIADGTGDTDPAG
                     EKPDETPTGVRDPAGRDGLPAQEADDGQTEPDEPAEPVKPYNVMDIDFDTLMASGESD
                     PLYPMHKYFSSVEPTPTNEYTGIFKGCNLIMFTAEGFSPYAVSPELTPTLYKMINEGF
                     VFRNFYTPIWWVSTSDGEYVACTGLIPKGGVWSMAKSGSNYMPFVMGNQFRKLGYLTK
                     AYHDHTYTYYSRDISHPNMGYDYKGVGNGLEVKKTWPESDLEMIEATVGEYINNQPFH
                     TYYMTVSGHMNYNFYGNQMASKNRKYVEDLPYSDASKAYIACNLELEFAMRELMDRLE
                     EAGIAENTVIVLSADHYPYGLPKENIDELAGHEVETNFEIYRNHLIIYKKGMDPVIVD
                     KPCDSLDIIPTLSNLFGLEYDSRLLMGEDILSSAPPLVIFSNRSWITDRAMYDSTRGK
                     VTFFDGTEKDDEYVKQINRKVANKFKYSAKILETDYYGKVFKVSKQQKASGD"
BASE COUNT         1619 a         2150 c         1754 g         1956 t
ORIGIN      
        1 tgtctccctt catgaacaag ggattcgtaa ccttcttctc cctgaccagt tccttcatct
       61 gccgcctgat gttttcatcc ctggtgtatc tgaaagccac ccccagcggt atgacggtcc
      121 agagattcct tgtgtccgcg atctccagct gcaggttttt attgtatata cgatcttcga
      181 ctacatattt cgcaagattg ttcccgcctg cagtcacata gtagttgctt cgtccctgcc
      241 tgagattcat ctcgagtatc ttgtacctgc cgtccctccg gtcgtacttg atgtcgaagg
      301 tcgaaaagcc cgtatagccg atactgtcga gaaaattcct caacttttcc gaaagcccgg
      361 cgtcatgatc attcatgagc acggcggtat tgccgatacc gtgcggggtg tgctcctcca
      421 gcaccacgtg ggcgaaagac atcagcttca cctttccgtc cctgcctgaa tagcagatca
      481 ttacccgcag gttagaatca tcccccggca cgaaatcctg tatgatgagc cggtcgtcat
      541 aacccgcccc gtatatctgt tctatgactt ccttcagttc gtcttcactg ttcagcctgt
      601 acgccttttt ctgcgtcggg aattcgtgct gccagtacct gatgccgttg gacggcttca
      661 ggaagaccgg gaaaccgaac ggcagcgtga aatcgcttcc catggatctt tcgtagatga
      721 acgtgtccgg atattccacg ccatggctgt cacacatcct gtaaaactcg gtttttgtca
      781 gcagcctgtc catgagctgc tcgtccacat atggcgcgac gatgttctcc ggcagcctgg
      841 cagaattcct tattatctgt tccacataat tatcgccgca gcccatgaga atgacttttt
      901 tttcagcaaa ttccgctgcc gtttttctta tggtttttat gaatatctcc tctgtgtcga
      961 gtcccggcac atgacggaaa tcgattatcc tgctgttgca gctgggcccg gtggcggctt
     1021 tcccgatcac caccgacctg accccgtaag cttcataaaa tgctcttgac atgccgtatg
     1081 tattgatgtc gtttccaagt aatacaggta tgaattctct ttcctggatc ttcaaaatat
     1141 atgtacccct ctgtcctccg tatttgttgt atgatcccgt ctttttctgt acataccacg
     1201 ttcattttag tataacaggt cccaaagtgc aagataaagt attccggtcg ccgtcggcgg
     1261 tccgccggcc gcaccggcag ccttgcctct cctttaagtc ttgccataaa aatcccgtta
     1321 cattactata taatcaggat catttcggaa tgagggggta tcggatatgc ccaaaccttt
     1381 caagggaccg gatatgccaa ggcctttcag tctcccggaa ggtttcctgc ttggcagcgc
     1441 cacagcggcg acccagatag aaggcggcga ccgcaacaac aactggtacg catggtgcga
     1501 gaaagggaag ataaaagacg ggacatcctg ccttcgcgcc gacgaccact ggaacaggta
     1561 catcgaagat atcgaactgc tgaagcggct ggatcacaag gtataccgca tgggactcga
     1621 atggagccgt atcgaacctg agaaagggaa attcgacggt gctgccatag cccgttaccg
     1681 tgacgaactt cagcggctta tccaaaacgg catacgccct ctcgtgacac tccatcattt
     1741 tacgcatccg ctgtggttct gcgctgaagg cgagttcgaa tccgaaaaat caatcgggta
     1801 tttcgagcgt tatacaagat atgtcgttga aaacatagga gacctggtca gcgagtatat
     1861 caccataaac gaacccaacg ttttcgttac aaacgggtat ttcatgggcg aatggcctcc
     1921 cggcaaaaag gatctcggcc ttgccatgaa ggtttacaga aacatggcat tgtgccacat
     1981 agccgcatac aaggcgatac atgaagtgag aagggaaaag ggctttgaca gggaaagccg
     2041 taccatggta ggggtagcca atcacctgcg cgtattcgac ccgtattcca ggaacccgct
     2101 cgactggctg gctgccaggg tcatgtgctg gctgttccag ggagcgatca tcaggtccat
     2161 gtcctccgga aggctcaggt tccccctggg ttccggagcg cctctcggta caggcaggtt
     2221 ctacgatttc atcggcatca attactacac caggagcgcc gtcaggttca agggcttcag
     2281 gagcgatgta atgcccggga ccccgaggaa cgacctggac tgggagatat atcccgaagg
     2341 gctttatata ctttgcaggg aattttataa aaaatacagg gcgccaatct ggatcaccga
     2401 aaacggcacc tgcgacggca atgattcctt cagggcagga tacatctacg accacctgta
     2461 tatagtgtcc atgctgtgcg gggaaggcat cccggtcgaa aggtactacc actggacgct
     2521 tatggacaat tttgaatggc tcgaaggaga atccgcccgc ttcggactgg tgcacgtgga
     2581 ttatgagacc caggcgcgca ccgtcaggaa aagcggaaga ttctactccg agatatgccg
     2641 gaacaacggc gtgacagagg aaatgatccg ggagcatctg ctggagtaag accgcagctg
     2701 gctgttccgg caagactgct aaggtcagat cacttcagtt ggtactttca aggtctctca
     2761 gctgctttgt gagcctccgg aactcctctt caagcgcttc cctgtcggcc ggctgcagtg
     2821 agagttttgt gaccacctcc gttatcctca tacggagcat ggtcttttca agttccatgt
     2881 ttttggcccc gtcagcagtc tttatgctct cctcgtactc atccaggccg ccttcgaaag
     2941 acctgacatc cagtccgccg gtcaccagca gcctgtctgc gactgcacgg acaaaagccc
     3001 tgtcgtgcga gacgaacagg accgtcccct cataatcctt aaggacattc tccagtgcct
     3061 ctatggactg tatgtcaaga tagttggtcg gctcgtcgag aagcaggaca ttggccctgg
     3121 acacgagaag ctttgcgaat gagaccttta tcttttctcc tccgctaagt attccaactt
     3181 ttttgaacac attggcgccc ggcagcagca gcctggccag tatcgtcctt gcaactgtct
     3241 cgttctgcac gctgtctttc ataacgttct ccaggactgt cttatcaggg tcgaggtttt
     3301 cgaatgtctg cctgaagtac gccagccttg ccctgggcac gatgtttatg cacctggcgg
     3361 ctcccgctgc agccctgtca gccagccaca tatacggatc aaaacccgtg ccggcgtctg
     3421 cacctgtact tgtaccagca cctgtatgca tgctcataac ctgtcctgcc gttcccgcaa
     3481 actcctcagg cataatacca gtcctgcgcc cggctgtatc cggatgtccc tggtccggca
     3541 ccggtactcc ggtgccgtaa ctgccgctgc gtgccaggta agctgtatat accaggttga
     3601 gcagggtggt ctttccggtg ccgttttctc cccaaagcgc tgttttcata ccgttggtga
     3661 tcctgaaacc ggcatttttg aatacgggca catttccata gctgaagctc agcctgtcgg
     3721 cacttatgat gattttgttc tccggaggtt cagtcaggga aagatccagc cttatcacag
     3781 gctgttcctt cggcctttcc tttacctcca gcttttcaag cctggttatc aggctgcttg
     3841 cggcactgtg cagcttctcc tggctttcag ccgcatggcg cttgtggagc cttgcctcgg
     3901 agtttcccat ccttgacggg gccttgcgga ctttccctgc ccttctcatc ctgtcggata
     3961 tggcctgttc aagggccgtt ttctcggcaa tatatttttc atactcggca gcggcccttt
     4021 gcctttccgc ctcgcactgc tccctgtaaa acgaatagtt gccgttgaag atccgtatgc
     4081 ctccgtcctt cacctcgatt atcctgttgc agagagcatc cagcaggctt cggtcgtggc
     4141 ttaccataat gaaggattcg actttcccaa gcttttgcct gaacagttcg atccccttgt
     4201 aatcaaggtt ggaggtcggc tcgtccgcaa acatcagcac ggtttccctg ctgaaagctc
     4261 ccgcgatttt aagccttgtc tgttctccgc cgctaagggc cggcctgtcg agtttaccgc
     4321 cgacttcgaa ttcactgagc agcttcatgg ctgctccggc cgcttctgta cgcagctgac
     4381 ggatatcgct cttcccgtcc atgccgaact gccttatata tccgacctcg caataccgcc
     4441 ttacataacc ttcatccggt tccaggcttc catacagcat atccagcagc gtagtcttgc
     4501 ccgagccgtt cagtccggtc acgccgatac ggtcaccgga atatactttc agttcgtcaa
     4561 atcctataac aagcctgtcg ccgtaatact tcttaacgtt caccgcttcc aacaacagca
     4621 aaaaagatct cccctttcac cggccagtgc aggaaagacc ttatagcgtt ccgtcatgat
     4681 ataccatccg acgatttgtc cctgcccggg aacaacaggt cccatacaat aagcaaaacg
     4741 tccaggtaaa gcaatcaaac ctgttttgaa actgtttcag cagttgcccg aataatactg
     4801 ttcctgttgg aaaatgcaaa gacggcaagg tatataaaag ccaatccggc actaaccgga
     4861 gcaatgatgc ccagtcatat aaactgtttg ttttgccgga tcagcgaatc ctcattacgt
     4921 tataccttac cctttcatag aattttctga atgttggaat atgtttcacc tgcaactcat
     4981 tttaccgcgt atccgtctta tcgtcaatac tcgcacattt gcagacaaac tcatattcaa
     5041 aagattattg cactgtataa caggccgagc gctcaactgt aagtgatcgg cctggcagtc
     5101 acaatgaaaa gcccagcacc ttagcaagct tgtccctgtc gacgtttttg ccgatgctca
     5161 tacccgtatc caatataaga tcgaccgcat cctcccggtc catcttctgc tttccgccgc
     5221 accactgcgc cggcgcccag tcttccaccc tctcatagac gttgatatgc cacccgtatg
     5281 ggcggccgtt cctgtcgatc ttctgcctgc ttccgccaac ggttatgtaa aactgcttct
     5341 gaagctcgac tattgccgaa tcgaccttgc tgctgccgct cttcttccct cttacaccca
     5401 tatggctcct tatctcgctg gtgtcgagca cctttttcca ttcgaacaat tgccacaggt
     5461 cccagcaggc ctggctgacc agcccttcgt atctcctctc ccgcatcgtc cgcgaaggtt
     5521 tgcatgcagc aagaaacacg gcatacatgt gaggcgcgat gaatcccttg ttcccgccca
     5581 gtacgcagcc gaatgccagg cgtttctcct cagcggccct gtccttccag cgccacgggt
     5641 ccgtttccgg atcaccggta tgccatgccc aatacggcgt ctcagatacc agggaaggca
     5701 tcccgtccat cacattggag aaaaacataa agccaagttc gttgaccctg ctgataaagt
     5761 cttcatattt cagaagctgc ataacgcatc tcctttcgat atagcgtgga tcgtgcgatc
     5821 cggactccgg cgggtttttc ggacatgcgc ctgttcccgg caaaatacgc acatgatacc
     5881 ggcaaggcac acatctgtat ccggcatgca cacgcagctg attccgggaa gacatacgac
     5941 ctgctccggc agccgatcac cagggccata tcagtctccg gacgctttct gctgtttgga
     6001 aactttgaac acctttccgt aataatcggt ctcaagtatt ttcgcggagt acttgaactt
     6061 atttgcgacc ttcctgttga tctgcttcac atactcgtcg tctttttcag tgccgtcgaa
     6121 gaatgtcacc tttcccctgg tggaatcata catagcccgg tcggtgatcc agctcctgtt
     6181 cgagaatatg accaaaggcg gcgccgacga aaggatatcc tctcccatca gcagccttga
     6241 atcatactcc agcccgaaca ggttggaaag ggtgggaatt atgtccaggc tgtcgcaggg
     6301 tttatccact atgacaggat ccatgccttt tttgtatatt atgagatggt tcctgtagat
     6361 ctcgaaattg gtttcgacct cgtggccagc cagttcgtcg atattttcct tcggcaggcc
     6421 gtacggatag tggtcggcgc tgagcactat gaccgtgttt tcagctatcc ctgcctcctc
     6481 aagcctgtcc atcagctccc tcattgcgaa ttccagttca aggttgcagg cgatgtatgc
     6541 cttggaagcg tcggaatacg ggagatcctc cacatacttc ctgttcttgc tggccatctg
     6601 gttcccgtag aagttgtagt tcatgtgccc gctgactgtc atgtaatatg tgtggaacgg
     6661 ctggttattt atatactcgc cgactgtggc ttcgatcatc tcaaggtccg attcaggcca
     6721 ggtcttcttt acctccaggc cgttccccac tcccttgtaa tcatacccca tgttgggatg
     6781 ggatatatcc ctgctgtaat aggtatatgt gtggtcgtgg tatgcctttg tcaggtagcc
     6841 cagcttcctg aactggttgc ccattacgaa cggcatatag ttgctgcctg actttgccat
     6901 gctccagact ccgcctttgg gtatcagccc cgtacatgcc acatattcgc cgtcagacgt
     6961 gctcacccac catatcgggg tatagaaatt cctgaacaca aatccctcat ttatcatctt
     7021 atacagtgtg ggagtaagtt ccgggctcac agcatacggc gaaaagcctt ccgcagtaaa
     7081 cattatcagg ttgcatccct tgaatatccc tgtatactca ttggtcggag tcggttcgac
     7141 agaggagaag tatttgtgca tcgggtacag cggatcgctt tcccctgaag ccatcagcgt
     7201 atcaaaatcg atatccatca cattgtatgg tttcacaggc tcagccggct cgtcaggctc
     7261 tgtttgtcca tcatcggctt cctgggcggg caggccgtcc cggccggcag gatcccggac
     7321 cccagtcggc gtttcgtcag gtttttcacc agccggatcc gtatcccctg ttccatcggc
     7381 aattcccggt tcttccgatc ccgcaggatc gtccggttca gatctcgcat acagcctgtt
     7441 caccagggtc tccttaagca cacctgttac catcctctc
//
LOCUS       DAOSCC010000116         7423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6427_length_7423_cov_4.491585, whole genome shotgun sequence.
ACCESSION   DAOSCC010000116 DAOSCC010000000
VERSION     DAOSCC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6427_length_7423_cov_4.491585"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..3431)
                     /locus_tag="PLG72_07220"
     CDS             complement(69..3431)
                     /locus_tag="PLG72_07220"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOJ80624.1"
                     /translation="MGLRIVYGRAGSGKSSFCYNEIRERASTDGNAGKAVPKLLLIVP
                     EQFSLQAEKNLARITGASGIYRAEVLSFRRLAYRVFSEVGGVTRRHIDSAGKSMLLFR
                     ILDRLGSELKVFSRAALRKGFIESLSDAITEFKRYDIAPEALLEAASGTEDGSLLKDK
                     LHDLSLVYSEFEKQLHQNYLDADDDLVELYKKLDLSTQYDGAEIWIDEFSGFTPQEYK
                     VIGKLLVKAARVTICMCTDCLSDDPNDSAPMVFGPVRKTAEKLLRLAEHLGVHVEKPV
                     RLDGVILRSRTDSTEASPADPAASAGPAASGRDRKVPDTGKKTSPSVATVSAGDPSKG
                     VSVRFIDSEAIGHLERNFWKYPYSRYTKQTDDIHITASANPYSEVEDAARDILRICRD
                     HGFRFRDIAVTMRNPDDYSSIIKSVFARYGIPYFLDGKRDIDGHPLIVFILSALEIFT
                     GNWSYEAVFRYAKTGLLSIGRQDLDILENYVLANGIRGSMWTREDDWDYPVGYDERKE
                     PSEREPVLLERINAARRELVAPLISFRAKTKGGARAVTFCTALYDLLCETGAAKRIEE
                     LSEMFADTGMLDRANEYRQVWNTVMDVFTQIVEVLGEETVGTERFSDILSVGFAGHKM
                     GLIPPSLDQVLVGSMERARSHDIKALYILGANEGVLPGNLGGDSLLSDSDRDSLARAG
                     LELPGNTQNRIREERFMIYMALATPSRYLRLSYPAADRDGRALRPSRVISEIKRIMPS
                     VTVKSNVVEDPGSLPQPSAPEPVFDEMVSRLRRLYDGNRIHDGWKRIYGWFNGRPEWK
                     DKCRAVLSGLDYTNQAKNLTKDRALKLYGNPVFTSVSRMETYAACPFSFFVKYGLKAK
                     ERKVFSFEQVDAGTFMHNIIDEFSRSLVKNGKSWRDIDEEWCRAEVSKLVDRYLSGKG
                     RNVLGSSKRYLYLSDRLKRTVVKSLMLISKHIAAGSFEPSGYEVEFGQNGRYPEITIE
                     LPSGEMIKLTGRIDRIDTMKGEDGTYLRIIDYKSGSRALKLGDVYYGLQLQLITYMDA
                     VLGQHIKAGSGSAEDDTAKDAGPAHGDPAGDTIKDGCATYSPDKAGEQAGENGKGAGK
                     LIPAGALYFRLSDPLIRCGRDSTEEEIEKAIM"
     gene            complement(3610..4977)
                     /locus_tag="PLG72_07225"
     CDS             complement(3610..4977)
                     /locus_tag="PLG72_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80625.1"
                     /translation="MMRFNGVMHGRTRNNIYTLPMLLVLTFILLMTACTETADNKYPA
                     GTAASNLSPDTQVYDSVSAADSTADKGADNVAAQAALPLSDVEGCSGAVFSSGGRLYV
                     YDAKSGSVSEIDEASGKKHFLGLSPDRKFAAWLYSFRSEDSYLTAEKIGIYEMETGRA
                     TELVLSEHLYQLMSVSWTSGRTIMAVGHLNPSTDMCQAFDAGEGRSLFVRPVGRLFDV
                     SPDGSRIIYYFTQHFSEAPLPPHLRISGVNTEDPDASAGFDGPVYEVGHEDDRIGQAC
                     FAGENKIIFIEYISGDARYNLKLAEIKEDSIEIVSEMPFSFAGLDVENMIAIEYFDDS
                     SKLYIISRDGSSASAAADINLHEFLLKDSEITRVNTVPTGLDASFAHDIVMSENGNGI
                     IITDTTGSGYKVFRKSYWYDGKEFTQILPDREVSDYDLCLLEEYAGEFLGTGPKMEID
                     GIEIY"
     gene            complement(5434..6627)
                     /locus_tag="PLG72_07230"
     CDS             complement(5434..6627)
                     /locus_tag="PLG72_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80626.1"
                     /translation="MNDNVGLEKILAEHFKRYPCMQIQDMVKLIYQNEFGGGHMVSDM
                     EESLVRLRDEYAYITGMEGRPPVSGAIGETGKDRDGAAGTDKCGPDGICSEPADGGQL
                     FEDIGNGLCRIHLAELGRHPVSLETVNNFFVHTANSHKGDIRSFENKLEVLRDCCERG
                     AMPFSTSDLDKYIRSYREQGYPVVSHSEIYRETYHPSYRIVESGFCRYFEVFCRIDEL
                     LKRVDSGLYRRDYITVAIDGNCCSGKSSLAALLEEIYDCNVFHMDHFFLPPELRTEER
                     LNETGGNVDYARFRNEVIRGLESGGGFEYGIYNCSIQAIERTVRVTAKKLNIVEGSYS
                     MHPTLSDHYDLKVFLTVEADEQSRRILKRNGPAMHRRFMEEWVPKENKYFEEFGIASK
                     CDIVL"
     gene            complement(6620..7150)
                     /locus_tag="PLG72_07235"
     CDS             complement(6620..7150)
                     /locus_tag="PLG72_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ80627.1"
                     /translation="MRSSSVRRLVLSGMFLALGLLLPFLTGQIPSVGSMLLPMHLPVL
                     VCGFVCGWPYGLAVGFITPLLRYMLFGMPPMPNALAMAFELAVYGFCTGLFYKLLPKK
                     NVFIYASLIISMICGRIVWGIASLIIYGLQGKAFTWQIFAAGAVVNAIPGIILQVVLI
                     PLIIIALKRGNMLRYE"
BASE COUNT         1667 a         2129 c         1709 g         1918 t
ORIGIN      
        1 gaaatatccc cgtccatgct cctgatcagt ttcacgtccg caagcaccag gcctttcatc
       61 cgcaactcct acattatggc cttctctatc tcttcttcgg tgctgtccct cccgcacctg
      121 ataagcgggt cgctgagcct gaaatacagc gctcctgcgg gtataagctt tccggcgccc
      181 tttccgtttt cgcccgcctg ttcaccggcc ttgtcaggcg aatatgtggc gcagccgtct
      241 tttatcgtat cgcctgccgg atcgccatgt gccggtccgg catcctttgc cgtatcatcc
      301 tctgcgctgc cggatcccgc ctttatgtgc tgtcccagta ccgcgtccat atacgtgatc
      361 aactggagtt gaaggccgta atacacgtct ccaagcttaa gcgccctgct tccggacttg
      421 tagtcaatta tcctgagata ggtgccgtct tcgcctttca tcgtatcgat cctgtcgatc
      481 ctgccggtca gtttgatcat ctcgcctgac gggagttcga tggtgatctc gggatacctg
      541 ccgttttgcc cgaactccac ttcatatcct gaaggctcga aactgccggc agctatatgt
      601 ttgcttatga gcatcagcga tttcacgact gtccttttca gcctgtccga cagataaagg
      661 taccttttgc tgctgcccag cacattcctg cctttcccgg acaggtacct gtccacaagt
      721 ttcgagacct cggccctgca ccactcctca tcgatgtctc tccagctttt cccattcttt
      781 acgagcgatc ttgagaattc atctatgata ttgtgcatga acgtgcccgc atcgacctgt
      841 tcaaagctga agacctttct ctcctttgcc ttcaggccat atttgacaaa gaatgaaaat
      901 gggcacgctg catatgtctc catcctggaa acgctggtaa aaacaggatt gccgtataat
      961 ttcagagccc tgtccttcgt gaggttcttt gcctggttcg tataatcgag cccgctcagc
     1021 acagccctgc atttgtcttt ccactccggt ctgccattga accagccata tatacgcttc
     1081 catccgtcat gtatcctgtt tccgtcgtaa agccggcgga gccgggatac catttcatcg
     1141 aataccggct caggcgccga aggctggggc aggctcccgg gatcttccac gacattgctt
     1201 tttactgtca ccgaaggcat tatccttttt atttcagata tcacacgtga aggccggagg
     1261 gcccttccgt ccctgtcggc cgccggatag ctcagccgca ggtacctcga cggggtcgcc
     1321 agcgccatgt agatcatgaa cctctcttca cggattcggt tctgagtatt gccgggcaat
     1381 tcgagccccg cccgggccag gctgtccctg tcgctgtcgg aaagcaggct gtcgccccca
     1441 agattccccg gcagcacgcc ttcattggcg ccgaggatat aaagcgcttt tatatcatgg
     1501 ctccttgctc tttccatgct cccgacaaga acctggtcga gcgaaggagg tataagcccc
     1561 attttgtgcc cggcaaagcc gacagacagt atatctgaaa agcgttctgt cccgacggtc
     1621 tcttcgccga ggacttccac gatctgtgtg aacacgtcca ttaccgtgtt ccagacctgc
     1681 ctgtactcgt ttgccctgtc gagcatcccg gtatcggcga acatttccga aagttcttct
     1741 atcctcttcg cagcgccggt ctcacagagc aggtcataca gggcggtaca gaaagtgacg
     1801 gccctggcgc cgccctttgt tttggccctg aatgaaatga gtggggcaac aagttccctc
     1861 ctggccgcat tgatcctttc cagcagtaca ggttcacgtt cggacggctc cttcctttca
     1921 tcatacccga cagggtaatc ccagtcatcc tcgcgcgtcc acatgctgcc cctgatgccg
     1981 ttggcaagga catagttttc gaggatgtcc aggtcctgcc ttccaatgga aagaaggcct
     2041 gtcttcgcgt acctgaacac agcctcgtac gaccagttgc ccgtgaaaat ctcaagtgcg
     2101 gacaggatga acactataag cggatggccg tcgatatccc tcttcccgtc gaggaagtac
     2161 ggtatgccgt aacgggcgaa aacgcttttg atgatactgc tgtagtcgtc cgggttcctc
     2221 atcgtcacgg cgatatccct gaacctgaaa ccatggtccc tgcatatcct gaggatgtcc
     2281 ctcgcggcat cctccacttc cgaatatgga ttggcagacg ccgttatgtg tatgtcatcc
     2341 gtttgcttcg tgtatctgct gtacgggtac ttccagaaat tcctttccag gtggccgata
     2401 gcttcagaat cgataaaccg tacggatacg ccctttgacg gatcgccggc agaaaccgtg
     2461 gcaaccgaag gtgatgtctt tttaccggta tccggcacct tcctgtcccg gcctgatgcc
     2521 gccgggcctg cagatgctgc cggatccgca ggagaagcct ccgtactgtc agtcctgctc
     2581 cgcaatatca cgccatcgag cctaacgggc ttctccacat ggactcccaa atgttccgcc
     2641 agccgcagaa gcttttcggc ggttttcctt accgggccga aaaccatagg cgcactgtcg
     2701 tttgggtcgt ccgaaaggca gtccgtacac atgcaaatgg tgaccctggc tgccttgact
     2761 agcaatttcc ctatcacttt gtactcctgc ggagtaaaac ccgagaattc atctatccag
     2821 atctcggcgc cgtcgtactg cgtcgacagg tccagttttt tatacagttc cacaaggtcg
     2881 tcgtcggcat ccaggtaatt ctgatgaagc tgcttctcaa attcggaata gaccaatgac
     2941 aggtcgtgaa gcttgtcctt cagcagggag ccgtcttctg tcccactggc tgcctccagc
     3001 agtgcttccg gcgctatgtc ataccttttg aattccgtta tggcatcgga cagggactcg
     3061 atgaatccct tcctgagagc ggcacgtgaa aatactttga gttcgcttcc gagcctgtcc
     3121 agtatccgga acagcagcat gctttttccc gcggaatcga tatgccggcg ggtcacaccg
     3181 ccgacctcgc tgaacaccct gtatgccagc ctccggaagc tcagcacttc cgccctgtat
     3241 atcccggaag cgcccgtgat ccttgccagg tttttttcag cctgcagcga aaattgctcc
     3301 ggaacgatca gcagcagctt cgggactgcc tttcctgcat tgccgtccgt acttgctctt
     3361 tcccttatct cattgtagca aaaactggat tttccgcttc cagccctgcc gtaaactatc
     3421 ctgagcccca tatccccctc cgttaacaac aacgtcgcac aaaagccggg caactgctat
     3481 atccgctcat gtcaaccgtt cttcatagtg cgatatatct ccccatttga cagacttcca
     3541 tccggtcata catatttcaa taatgtgtta tctttcggga taacggcgat atcacgccga
     3601 taccagcagt caatatattt ctatcccatc gatctccatc ttcggaccgg taccaaggaa
     3661 ctcccctgcg tattcctcca gcaaacacag gtcataatcg gacacttccc ggtccggcag
     3721 gatctgcgtg aactccttcc cgtcatacca gtatgatttt ctgaagactt tgtaaccgga
     3781 tcccgttgta tcagtaataa tgataccgtt tccgttttca ctcatgacga tatcatgcgc
     3841 aaaagatgca tcaagccctg tcggcaccgt atttaccctg gttatttccg aatctttcag
     3901 caaaaactca tgcaggttta tatcggcggc cgccgaggca gaactgccat ccctgcttat
     3961 aatataaagt ttggaactat catcgaaata ctcaatggcg atcatatttt ccacatcaag
     4021 gcctgcaaaa ctgaaaggca tctcggatac gatctctatc gaatcctctt ttatttccgc
     4081 cagtttaaga ttataacgcg catcaccgga tatatattcg ataaagataa tcttattttc
     4141 tccggcgaag catgcctgcc cgatcctgtc atcttcatgc ccgacctcat acacaggtcc
     4201 gtcaaaaccg gcagaagcat caggatcctc cgtgttgacg cccgaaatcc taagatgcgg
     4261 cggcagcggc gcttcagaaa aatgctgcgt gaagtaatag attatccgcg atccgtcggg
     4321 ggatacatcg aagagcctgc cgacaggccg aacgaacagg ctcctgcctt ctccggcatc
     4381 aaatgcctgg cacatgtccg tggacgggtt caggtggcct acagccataa tggtccttcc
     4441 ggatgtccag gaaacgctca tcagttgata caggtgttcg ctcaatacaa gttccgttgc
     4501 tctcccggtt tccatttcat aaatgcctat tttctccgca gtcaggtaac tgtcttccga
     4561 cctgaaggag tacaaccagg ctgcaaattt cctgtcaggc gacaatccga gaaaatgctt
     4621 tttccctgaa gcctcgtcta tttcgctcac cgatccgctt ttggcatcat agacatacag
     4681 cctgccgccc gaactgaata ccgcgccgct gcatccttcc acatcactga gcggaagggc
     4741 tgcctgtgca gccacattgt ccgcgccttt atccgccgta ctgtcagcag cactgacaga
     4801 gtcataaact tgggtatccg gtgaaagatt tgatgcagcc gttccagcag gatacttgtt
     4861 gtccgccgtt tcggtgcacg ctgtcatcag cagtataaaa gtcaatacca gcaacatcgg
     4921 cagtgtatag atattgtttc gtgtacgtcc atgcattacg ccgttgaacc tcatcatcga
     4981 aaactttatc ggaactatta tacagtcgac atatgaatgt tcttcatcaa taatttattc
     5041 taacatatgg acatgcagtt gaaaatcgat aatgatattc ggaaatgcgg cgccatggta
     5101 aacattgata taataaaacc gtcagttaca gccgattgca ggatatcgtg ccattatcga
     5161 cgccgggttc aggatcgaaa gagtggaaga gccttatccc ccggaggact ggaagcaaaa
     5221 atacgtcagc cgatataatg cgtttatcga aacaccaagt tatatgattt taagattgtc
     5281 aaaataaagg ttaagtacat cgggcgattg cccgccaatg gcaggctgtc tgaaaacagg
     5341 ccataaaggg cggcattgaa acgtgctgcg ctatatagtg ttatatagcg ctatattgtt
     5401 atgcagcggt ataagcctgt gactatatcg ggcctataac actatatcgc atttcgaagc
     5461 tattccgaac tcttcgaaat acttgttttc cttcggcacc cattcctcca tgaaccggcg
     5521 gtgcatggca ggcccgttcc ttttaaggat acggcggctc tgctcatccg cttccacggt
     5581 aaggaagact ttgagatcgt aatggtccga cagtgtggga tgcatactgt atgagccttc
     5641 gactatgttg agttttttgg ccgtgacccg tacagttcgc tctatggcct gtatggagca
     5701 gttatatatg ccgtattcga atccgccgcc gctttccagt ccgcggatga cttcgttcct
     5761 gaacctggca tagtccacgt tgccgccggt ttcattcagc cgttcttccg tcctcagttc
     5821 gggcggaagg aagaagtgat ccatgtggaa cacattacag tcgtatatct cttccagcag
     5881 cgctgccagt gaacttttgc cggaacagca gttgccgtct attgcgaccg ttatataatc
     5941 cctgcggtat agacctgaat ccacgcgctt cagcaattcg tcgatacggc agaaaacctc
     6001 aaagtacctg caaaagccgc tttcaactat cctgtatgaa ggatggtatg tttcccggta
     6061 tatttcgctg tggctgacca caggatagcc ctgttcgcga tacgagcgga tatatttatc
     6121 caggtcgctg gtgctgaacg gcatcgcgcc tctctcacag cagtctctca gtacttccag
     6181 cttgttttcg aagctcctga tatcaccttt atgggaattg gcagtgtgga caaagaaatt
     6241 gttcacagtt tcgaggctga ccggatggcg gccgagctct gcaagatgga tcctgcaaag
     6301 cccgttgcca atatcctcaa aaagctgccc gccatcagca ggttcgctgc atatgccgtc
     6361 cgggccgcat ttgtccgttc ctgccgctcc atccctgtct ttgccggttt ctccaatagc
     6421 gccggataca ggcggcctgc cctccatgcc tgttatatat gcatactcgt cccggaggcg
     6481 gaccaggctt tcctccatgt cgctgaccat gtggcctccg ccgaactcgt tctgatagat
     6541 cagcttgacc atatcctgta tctgcataca gggatatctt ttaaagtgct ctgcaaggat
     6601 tttttccagt ccgacgttgt cattcatatc tcaacatgtt acctcgtttc aatgcgataa
     6661 tgatgagcgg tatgaggact acctggagta ttatcccggg gatggcgttc actacggcgc
     6721 ccgcagcaaa tatctgccat gtgaaagctt taccttgcaa gccgtagatt atcaggctcg
     6781 ctatgcccca gacgatccta ccgcatatca tcgagattat aagtgacgca tatatgaaca
     6841 cattcttctt cggcagcagt ttgtaaaaca gcccggtgca aaaaccgtaa acggccagtt
     6901 caaaggccat tgccagcgca ttcggcatgg gcggcatccc gaaaagcatg taccgcagca
     6961 gcggtgttat gaatccgacc gcaaggccgt aaggccagcc acatacaaat ccgcacacca
     7021 ggaccggcag gtgcatcggc agcagcatcg aaccgacaga cgggatctgc cctgtcagga
     7081 acggcaacag cagccccaat gcaagaaaca tcccggacaa tacaagtctt cttacagatg
     7141 atgacctcat actcaattcc tcccttacca atgtaaaacg atcagcggaa acgcaaaaac
     7201 tgcacaaaaa aaccacgcag tcggcgtttc ataaagaagc cttcttcatg aaagcaaata
     7261 ccttcgtggt atcattctgt acgtatgcac agttcatatg acgttttggg attcatcccg
     7321 ctgtaacgga taacgtccgc tgtcaggctg cttaccggtt ttggaggtct ttcccggttt
     7381 ttgacgctgc cgccgtcgtt acataccggc ttcagccggc tgt
//
LOCUS       DAOSCC010000117         7418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6435_length_7418_cov_4.337634, whole genome shotgun sequence.
ACCESSION   DAOSCC010000117 DAOSCC010000000
VERSION     DAOSCC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_7418_cov_4.337634"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLG72_07240"
     CDS             complement(<1..292)
                     /locus_tag="PLG72_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80628.1"
                     /translation="MENNRYLLKLFAALAILILAVNAAVLHFGFGIRFSDLLDFVVHV
                     VGDGSAKTFDDPAGNRSSERNGLSGMGDGSSEMLTLDRGWTGEGGDSPAESWD"
     gene            complement(389..1258)
                     /gene="galU"
                     /locus_tag="PLG72_07245"
     CDS             complement(389..1258)
                     /gene="galU"
                     /locus_tag="PLG72_07245"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOJ80629.1"
                     /translation="MKIRKAVIPAAGMGTRFLPATKAQPKEMLPIVDKPTIQYIIEEA
                     VASGIEDILIVSGRGKRAIEDHFDKSYELEDMLKRKGEEELLSLVQDISNLANVNYVR
                     QKEPRGLGHAIYCARSFIGNEPFAILLGDDIVDSSVPCLKQMIDIYEKYNTTVLGVQQ
                     VPESEVSKYGIIKGTLIEDRVYKVEDMVEKPDIDSAPSNVAILGRYIVTPEVFTHLEN
                     ASPGKGGEIQLTDALKAMLAANPIHAYDFIGKRYDVGNKLGFLEATVEFALKRDELRD
                     EFAAYLRNRINCL"
     gene            1575..2399
                     /locus_tag="PLG72_07250"
     CDS             1575..2399
                     /locus_tag="PLG72_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ80630.1"
                     /translation="MKHFEFKAFAVLNEIDLNKIAVNCGIPKKYTWEEPLILDHKLLG
                     NILGRRYDADSKVLIFSFGSIVLVNVPEDIMPQLLGFIKKYVPGVDLGNWNRYTDEYE
                     LRTEPQSSLEITDEYAVVPRYELFYIELAAIVIAKSVAMEKTEDELGKIFDQLESTID
                     RLEHGQLRIGDRELARATAQVTRHQYNTISYIMILDKPDITWSNSDASLFYEKMSDIF
                     ELNDRYTILTKKADIMNNIISGFSSISHSIRGLFVEWIIVLLIVFEVILMVIDLIL"
     gene            2551..3447
                     /locus_tag="PLG72_07255"
     CDS             2551..3447
                     /locus_tag="PLG72_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HOJ80631.1"
                     /translation="METKHFISIKAYPAIDLNKTAEYFGLPVPEARERSLTIEGEKLS
                     EIFKYDTRQKRVHVFSFGCLVFEDMEIDETDRFLEIMQPITGEPDYRMLLRYRESHDS
                     TVTEDGSIGLWPESGAPAFPPGIELSHTIAAVLAKSAALSKEEEDLNTLLNEAYRYIN
                     RPGEGIFNAGTRMYAKTMTHIVRFEKESAAGFGIFDRPASTGRKLLLRDAYDKLANYY
                     ELDERYDLLEKKAEQLRDIVRSGSVERFRRQENRLLAFETFLLMLFPLSHILGTVFAR
                     SGVKDFINMIFTNLLQKLNIIN"
     gene            3737..4000
                     /gene="ffs"
                     /locus_tag="PLG72_07260"
     ncRNA           3737..4000
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG72_07260"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4299..6185
                     /gene="dnaX"
                     /locus_tag="PLG72_07265"
     CDS             4299..6185
                     /gene="dnaX"
                     /locus_tag="PLG72_07265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ80632.1"
                     /translation="MMSYIALYREWRPTRFSDVVEQEHVVKALRYSVSTGRIAHAYLF
                     CGTRGTGKTTMAHILSRAVNCLDPQDGEPCNQCEICREILSGSCVDVLEIDAASNNSV
                     DNVRSIRDEVIYAPTKARKKVYIIDEVHMLSTGAFNALLKTLEEPPEHVIFILATTEP
                     HKLPATILSRCQRFDFRRISQDSIAKHLAMIASANGTELDPNAAKLIARMSDGAMRDA
                     ISLLDQCMSLGRKSISYDDVLSVVGVVNDEFMTSFIDSMLAKNVEDILHNVSMLIAEG
                     RDAAHFTSDLVVYFRNLLICRSTNGKCDDLIDVPAEVLQTMKQQAEKLSLEEIMYHIR
                     ELSALENNLKYAANPRVLLEVTLIKLCRGIVSANAGIEERLAAIERRLQSGDFPYRPA
                     ADPYRPGDVSSDIPGTQGPAGTPGQADTSGSADISASPGSSAPLAGKSDRGSYGSGDG
                     MTVGGSGITPPGPDNIPVEADDTRRTPGKSFDADSTGKTAEKGHAGQKAPGGAGQKGQ
                     SRKAVDYDKWPQILEHLKSSGRMSIYLILLNVRAVLLDDETLGLVAKEKNVRSMVSMV
                     EHIRVIEDAVSKVAGRSMKIKCIDEEILHKAASDEKKESSDLLERARMLADKVGLPLE
                     IIDE"
     gene            6403..6744
                     /locus_tag="PLG72_07270"
     CDS             6403..6744
                     /locus_tag="PLG72_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ80633.1"
                     /translation="MAKHGFPGFGGANMNNLMKQAQKMQRDMEKLQAELQERIVEATA
                     GGGAVKVVATGKKEIKEITIDPDVVDPDDVEMLQDLILAAVNEALRKAEEMVSSEMAK
                     LTGGLGLGGLF"
     gene            6746..7348
                     /gene="recR"
                     /locus_tag="PLG72_07275"
     CDS             6746..7348
                     /gene="recR"
                     /locus_tag="PLG72_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ80634.1"
                     /translation="MNHLYSPPIARLIEELEKLPGIGHKSAQRLAFHILNLPAEKSEA
                     LANAIREAKLKTRYCSICSNITETDPCSICGSAKRDHSIICVVEDPKDVVAMERIREF
                     NGVYHVLHGVISPMEGIGPEDIRIKELLQRIRDGNIREVILATDPDVEGEATAMYISK
                     LLKPIGIRITRIAHGIPVGGDLEYADEVTLAKALEGRREL"
BASE COUNT         2005 a         1830 c         1863 g         1720 t
ORIGIN      
        1 tgtcccaact ttctgcagga ctgtccccgc cttcaccagt ccagcccctg tccagggtca
       61 gcatttccga agaaccgtcc cccatcccgg aaaggccgtt tctctccgaa ctccggtttc
      121 ctgccggatc gtcaaatgtt ttcgcagaac cgtccccgac aacatggacg acgaaatcaa
      181 gcaggtccga aaagcggata ccgaaaccga aatgcagcac tgcagcattt acagcaagta
      241 tcagtatagc gagagcggca aacagtttta aaaggtacct gttgttttcc ataaaataat
      301 acctgtaatt cttttacagg tattatttgc tatatgtatg agattctatt catcagcagc
      361 ggttgatttt tacgccgcgt acttaccgtc acagacagtt gatcctgttc cttagataag
      421 ctgcgaactc atccctgagt tcatcccgtt tcagggcaaa ttcgactgtg gcttcaagga
      481 aacccagctt atttcccaca tcatagcgtt tgcctataaa gtcataggca tggatcgggt
      541 tcgctgcaag catcgccttg agcgcatccg tgagctgtat ctctcctccc ttgcccggcg
      601 acgcgttttc caggtgtgtg aatacctccg gcgtgactat atatcttccg agtatggcca
      661 cattggaagg agcactgtca atatcaggct tctccaccat gtcttccact ttatatacgc
      721 ggtcttctat cagggtgcct ttgatgatcc cgtatttcga cacttccgat tccggaacct
      781 gctgtactcc gagaacggtc gtgttgtatt tctcataaat gtcgatcatc tgtttcaggc
      841 acggaacaga tgaatccact atatcgtctc ccagcaaaat ggcaaacggc tcattgccta
      901 tgaacgaccg ggcgcagtat atggcatgtc ccagccctct gggttccttc tgcctgacat
      961 aattcacatt ggcaagattc gagatatcct ggacaagtga caggagttcc tcctcgcctt
     1021 tccgtttcag catatcctcc agttcatatg atttgtcgaa atggtcctcg atagccctct
     1081 tccctcgtcc tgatacgatc aaaatgtcct cgatgcctga cgcgacagcc tcttctataa
     1141 tatactggat agtaggtttg tcgactatgg gaagcatctc cttgggctga gcctttgtgg
     1201 ctggcagaaa cctggtaccc attccggctg caggtatcac ggctttgcgt atcttcaatt
     1261 ccatctcccc ttcctgagaa atcttatccg aaacatgccc tgccgttcat aatcagcctg
     1321 aacggtttgt acatcagcat actttaatta tacaccattc ctggaatatt cggcgacgtt
     1381 gtcgtactat atatacctgg tacttggtat ggactccatt ttttatgata ttataatata
     1441 ggataccatc cagtggtcaa taattcaaca gtatgcatcc cggcggcagc ccgggcgggc
     1501 attcaccttg cggcgagcag cgctgccgtg atgaacggcg gcatgatgta tcctgcaaaa
     1561 catgggaggt gtttatgaaa cattttgaat tcaaggcttt tgctgtactg aacgagatcg
     1621 acctcaataa aatcgctgta aactgcggca ttcccaaaaa atatacatgg gaagaaccgc
     1681 tcatactgga ccataaactg ctcggaaata tactcggccg taggtacgat gccgacagca
     1741 aggtcctcat cttctctttc gggagtatag tactggtcaa tgtaccggag gacataatgc
     1801 cccaactgct gggcttcatc aaaaaatacg tgcctggcgt ggatctcggc aattggaaca
     1861 gatataccga cgagtacgaa ttacgtacag aaccccaaag ctcgctggaa atcactgacg
     1921 aatatgcggt ggtaccccgt tatgaactgt tttacataga actcgcagcc atagtcattg
     1981 ccaaatcggt tgccatggaa aaaacggaag acgaactcgg caagatcttc gaccagctcg
     2041 aatccacgat agaccggctc gaacacggcc aactgcgcat cggcgacagg gaactggccc
     2101 gggctacggc gcaggtgaca aggcaccagt acaacaccat aagctatatc atgatactcg
     2161 acaagcctga cataacgtgg agcaacagcg atgcatcgct gttttacgaa aagatgtcgg
     2221 acattttcga actgaacgac aggtacacta tcctgacgaa aaaagccgat atcatgaata
     2281 acataatcag cggcttttct tccataagcc attcgatcag gggacttttt gtcgaatgga
     2341 taatagtgct gctgatcgtc ttcgaggtca tacttatggt cattgacctc atactttaag
     2401 caaccgggcc gggcagcccg aaaaacggct ggcatgccag taaatacggc ttttacgcga
     2461 cccggctgca gccatggacc gatagctgca acagctcaat tctgattgcg gctgttcccc
     2521 agaacccaca gccatccgga ggtatcgtga atggaaacga agcatttcat aagcatcaag
     2581 gcttatccgg ctatagatct gaataaaaca gcggaatatt tcggcctccc tgtaccggaa
     2641 gcaagggaaa ggtcgctcac catagaagga gaaaaacttt cagaaatatt caaatatgac
     2701 acacgccaga aaagggtcca tgttttcagc tttggctgcc tcgtttttga agatatggag
     2761 atcgacgaaa cagacagatt tctcgagatc atgcagccga taaccggtga gcctgactac
     2821 aggatgcttt tgaggtacag ggaaagccac gattctactg ttacggaaga cggatcgatc
     2881 gggctttggc cggagagcgg cgcccctgct ttccccccgg gcatagaact ctcgcatact
     2941 atcgctgctg tattggccaa atccgcagcc ctgtcgaaag aggaggaaga cctcaatacg
     3001 cttctgaatg aagcttacag gtatattaac cggccgggcg agggcatctt caacgccggg
     3061 acccgcatgt acgcaaagac gatgacgcat atagtcaggt ttgaaaaaga aagcgccgcc
     3121 ggtttcggca tattcgacag accggcatcg acaggcagaa aactcctgct cagggatgca
     3181 tacgataagc tggcgaacta ttatgaactg gatgaaagat atgatctcct cgaaaaaaag
     3241 gctgaacagc tgagggatat agtaaggtct ggttctgtgg aaaggttcag gcggcaggaa
     3301 aacaggctcc ttgcttttga aacgttcctg ctgatgttgt ttcccttgtc ccacatcctg
     3361 ggaacggtct tcgccaggtc cggggtcaaa gactttataa atatgatttt cacaaatttg
     3421 ttacaaaaac ttaatattat aaactagaaa atcatggtac aatataattg ccacagccaa
     3481 taatactatt ttttcatttt tgttcctcct tttaatatag acgttggctg agtttagttc
     3541 atcttatctg aaaagaagca cttatattga ttcggaatca tttataaatt cataaagtgc
     3601 tttctttttt tgcccttttt tgaccgggac tggcacaaaa tgcttgtgtg ccgctgaatg
     3661 aaatgtgttg ataaggctaa gcatattgag tataataagt gagtaacact gtaaaccggc
     3721 attgttttaa atttggctgt gctagacggg gaggtagcgg tgccctgtac ctgcaatccg
     3781 ctgcagcagg gtcgaatccc tggtcgaggc ttgtttttgt gaggtctgcc ccgtacaagt
     3841 ggcgatgaca gttgggtctt gcgcaacgta agcccgtgaa ccccgtcagg tccggaagga
     3901 agcagcggta agcggatact ttcgtgtgcc gcaggggtgc ctggctggag tcaactatac
     3961 gggtcacgct cggaaaagta agtcgacacc aggtgcacag ccttcaatta taaatcatgg
     4021 tttctataaa aggtgcgtat cccgcacctt ttgctgtttc ccggagttcg ccctatacaa
     4081 gtggtctgtc cggccatcgg ggtttgcacg atatccggta ttcgtccaac ttcacgttcc
     4141 tgttttcgaa gggcggttca tgcaagcggg gtattttcac gtttctgagg ccgccaggaa
     4201 atttccgtat catggatatc cacggccgga ggactttttt ttggcctgcc attctgatat
     4261 actaagttca taaacaaggt tcggaagaaa ggctgttgat gatgtcctac attgcattat
     4321 acagagaatg gcggccgacc aggttcagcg acgtggtcga acaggagcat gtggtaaaag
     4381 cgctcaggta cagcgtcagt accggaagga tcgcccatgc ctatcttttc tgcggcacca
     4441 gggggacggg caagaccacg atggcccaca tactctcccg tgcggtcaac tgccttgacc
     4501 ctcaggacgg cgaaccttgc aaccagtgcg aaatatgccg cgagatactc tccggcagtt
     4561 gcgtggatgt gcttgaaatc gacgcggcat ccaacaacag cgtcgataac gtacgttcca
     4621 taagggatga agtcatctac gccccgacaa aagccaggaa aaaagtctat atcatagatg
     4681 aagtccacat gctttccacc ggcgccttca atgcgctgct caaaacgctg gaggagcccc
     4741 ctgagcatgt cattttcata ctcgcgacca cggaaccgca taagcttccg gctacgattc
     4801 tttcacgctg ccagcgcttc gatttcaggc ggatttccca ggacagcata gcaaagcacc
     4861 ttgccatgat cgcatccgcg aacggcacgg aactggatcc gaacgccgca aagctgatag
     4921 ctcgtatgtc cgacggcgcc atgagggatg ccatcagcct gctggaccag tgcatgtctc
     4981 tcggcaggaa gtcaataagc tatgacgacg tgctttccgt cgtaggtgtg gtcaacgacg
     5041 agttcatgac aagcttcatc gacagcatgc tggcaaaaaa cgttgaagat atcctgcaca
     5101 atgtgagcat gctaatcgcg gagggccgcg atgctgcaca tttcacttcc gaccttgtgg
     5161 tttatttcag gaacctgctg atatgcagat ccacaaacgg taaatgcgat gacctgatag
     5221 atgtcccggc cgaagtcctg caaacgatga agcagcaggc agaaaagctg tccctggaag
     5281 agatcatgta ccatatacgg gaactgtccg cactggaaaa caacctgaaa tatgcggcaa
     5341 accccagggt cctgctggaa gtcacactga taaaactgtg cagggggatc gtcagtgcaa
     5401 atgcaggcat agaagaaagg ctggcggcga tcgagcggag actgcaaagc ggagatttcc
     5461 cgtacaggcc tgcagcggac ccgtaccggc ctggtgatgt ttcatcggat atccccggta
     5521 cccagggacc ggcaggcaca cccggacagg cagatacatc ggggtctgct gatatatctg
     5581 catctccagg cagttcagca ccccttgccg gtaagtcaga cagaggttcc tacgggtcag
     5641 gtgacggtat gaccgtcggc ggaagcggca ttactccccc aggtcctgat aacatcccgg
     5701 ttgaagctga cgatacccgc agaacaccag gaaaaagctt cgatgctgac agcaccggca
     5761 aaaccgcaga aaaaggtcat gccggccaaa aagcccctgg aggagccgga cagaaaggac
     5821 aatcgagaaa agcagtcgat tacgacaagt ggccgcagat attggagcat ttgaaaagct
     5881 ccgggagaat gtccatttac ctgatactgc tcaatgtcag ggctgtcctg cttgatgacg
     5941 agaccttagg gctggttgcg aaagaaaaaa acgtaaggtc catggtatca atggtggaac
     6001 atataagggt catagaagat gcagtctcca aagtcgcagg acgcagcatg aagataaaat
     6061 gcatagatga agagatcctg cataaggcgg cctcagatga aaaaaaggag agcagcgacc
     6121 tgctggaaag agcgcgtatg ctggcagaca aagttggcct gccgcttgag ataatcgacg
     6181 aataggcacg gcatgatttt gcagatcggg cagttttgct gacaggtcct cggtgaccgg
     6241 gccatatgcc gaagggtagt tggatctgga ccctttggat atacggagcc tgacggcgaa
     6301 gcctgatgct tttgtcgtcc gcaaccagac acccttgaac ggccggatgc taatatatat
     6361 aatatacctg tcataccaaa taagaagacg gaggtgccaa ttatggcaaa gcacggattc
     6421 ccgggcttcg gtggcgcgaa catgaacaat ctcatgaaac aggcacagaa aatgcagaga
     6481 gatatggaaa aactgcaggc cgagctccag gaaagaatag ttgaagcaac tgccggaggc
     6541 ggtgcagtca aggtagttgc cacaggcaaa aaggaaataa aggaaataac gatagatccc
     6601 gacgtcgttg accccgatga tgtggaaatg ctccaggacc tcatcctggc ggcagttaac
     6661 gaagcactcc gcaaggctga agaaatggtc agttccgaga tggcgaagct gaccggagga
     6721 ttaggactgg gcgggttatt ctgacatgaa tcatctttat tcaccaccaa tcgcaagact
     6781 gatagaagaa ctcgaaaagc ttccggggat aggccataaa tcggcacaga ggctggcttt
     6841 ccatatattg aacctgccgg ccgaaaaatc ggaagcgctg gccaacgcga ttagggaagc
     6901 aaagctgaaa acgcgttact gttccatatg cagcaatata actgaaaccg atccctgcag
     6961 catatgcggc agcgcaaagc gtgaccacag catcatatgc gtcgtggaag accccaagga
     7021 cgtagtcgcc atggaaagga tcagggaatt caacggtgta taccatgtgc tgcatggcgt
     7081 catatccccc atggaaggta tcggtcctga agacatcagg ataaaggagc ttctgcagag
     7141 gatacgcgac ggcaatatac gggaagtcat actggccacg gaccccgatg tcgagggtga
     7201 agccacggcc atgtatatct cgaagctttt gaagccgata ggcatcagga tcacaaggat
     7261 cgcccacggt atccctgtag gcggcgatct cgagtacgcc gatgaagtta cgctcgcaaa
     7321 ggcgctcgaa ggccggcggg aactttaaac caagttttaa acttgtataa atcaaataaa
     7381 ctcctttcat tttgcaaatt atcgatagga agaaaata
//
LOCUS       DAOSCC010000118         7376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6474_length_7376_cov_4.959295, whole genome shotgun sequence.
ACCESSION   DAOSCC010000118 DAOSCC010000000
VERSION     DAOSCC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6474_length_7376_cov_4.959295"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLG72_07280"
     CDS             complement(<1..439)
                     /locus_tag="PLG72_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ80635.1"
                     /translation="MGIKYFEDQRVFKLDANNTSYIIGIVDDEDFIGHVYFGRRIGDG
                     HFSPESLMHTNEYPFLPSKNNRDRLSFYDAFPREYPTHGIGDFRETCLEIRDVDGHAA
                     SKLQYVSHRITGGKPKLDGLPATFGNEDDCTTLEIFCEDTVLKL"
     gene            712..2160
                     /locus_tag="PLG72_07285"
     CDS             712..2160
                     /locus_tag="PLG72_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ80636.1"
                     /translation="MAYRSCRPEDFDAFVARNENRLFRAALSMTGNASDAEDILQEVF
                     LRAYEKAPEFTSGEHEKAWLMKVTINLCKSRLRSPWHKRRVAMLESYPATEPEQHELM
                     EQVMSLPSKYRAVIYLYYYEGYSVKDISELTGQRESTVRSHLTRARQRLRSFLKEEVM
                     KAYKDYMDKITVSDALHRKLVSHASGNVRPVLIRRYAAAFACLALALVCTYAVTQLYQ
                     NNTPQEPGKDPYVTPGETPVAAKSGGSQSIAGHSNTASDESDTNPSVSQSDDTSSAGQ
                     PEGSPSVSKPDEAPSVPFQDESPSVADILVKRISDSAGTDVLLNNLSLDEARADADFG
                     AYLPKTVPSGFAFESATRYVDEKSNHLTALWFKGMRMIHWSVSKLDVEDKARITSVSD
                     TKNYDLALYPIPHAESVPEDLREIVDDPIFIIDDLTLDAVKARAYEVQDAGDISGPRM
                     SFSVLYGDTLIKLYVKGASPESVFDMLMQIKA"
     gene            complement(2532..3188)
                     /locus_tag="PLG72_07290"
     CDS             complement(2532..3188)
                     /locus_tag="PLG72_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOJ80637.1"
                     /translation="MSRTTKRLSLLLAVTVCIALAAAHAFASSSIQVMLNSLNILYNG
                     EKTDLDHFVYNNTAYVPLRKAAELFGKEVTWDQATRTVNISDKKTDGSYAESDAVSTA
                     LIANAAGNTIDLEFQVRNNAGKDITITLYYPFYDFAVYDESGNEVYRHSEEYPFSILP
                     IVDETINSGGKFTVNEKLDLSARDFAPGETYRVDFFVSFEMDSEMHKLSESAYFKIPG
                     "
     gene            complement(3345..6344)
                     /locus_tag="PLG72_07295"
     CDS             complement(3345..6344)
                     /locus_tag="PLG72_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOJ80638.1"
                     /translation="MLELKNITKTYHTAGEDVEALKGIDLQFRNSEFVCILGPSGCGK
                     TTLLNIIGGLDSYTSGDLIINGRSTKEYKDRDWDAYRNHSVGFVFQSYNLIPHQTVLS
                     NVEIALTLTGVPKKERRQRAREALEKVGLGDQLRKKPAQLSGGQMQRVAIARAIVNNP
                     DIIMADEPTGALDTETSRQVMDILKEIARDRLVIMVTHNPVLAERYSTRIVKMLDGRI
                     IDDSQPLTDEEYRKELDEAKKKKTETHKKKMPSMSFLTSFGLSLKNLFTKKGRTALTS
                     FAGSIGIIGIALIYAVSKGTTAFIDSVQEDTLASYPLTIEAQSVDMSSLISTFLGKAK
                     SVSEHEDDAVYQKAMIYEMMNSMNSLDTQKNDLKAFKEYIEAQRADENSKLHSALSGV
                     QYSYDLDLMIYTKNVDGSIIRSDRQTILTEVMKKYAKMDMSTMLALREQTVMGRTDSL
                     MKQSMGLWKELVPGDSGKPVNSVTEKQYDIIYGRWPSRYDELVLFVDENNEIDDLTLY
                     TLGLLSEKEIEILMDAALNKKTVDYEIRKWSYEDICNMDFRTILNCDCYTYDEKTGTY
                     TDLRETEAGLKYLYDNALKLRVVGIAKPDEDAISPMTNASIGYTYMLTDYIIDKANES
                     DAVKAQLADPSTDIFTGLPFRSTSGDQTESSRADEFRNYVSSLDTAGKAEAYKKIMSI
                     PSEEAVQEFVSGTLAGMTRADMEKAIAPAMAEQTGMDEETIAGYIASMSDEDLSELLV
                     KGLEEQFRAQYAAEAAKQMSAMSGEQLAAALDMTLEQFTDEQCAAYYDELVVFSDSTY
                     EDNLLRLGLVDRGSPAAINLYASSFANKDVIEEVIAEYNESVDDLKEIKYTDYVGLIM
                     SSVTTIINAITYVLIAFVAVSLIVSSIMIGVITLISVQERTKEIGILRAIGASKGNVS
                     SLFNAETVIIGFTSGLLGVLITYLLCIPINMILHHLTGLSNLSAFLPPEVAVMLVVIS
                     MLLTLIAGIIPSRSAAKKDPVVALRTE"
     gene            complement(6358..>7376)
                     /locus_tag="PLG72_07300"
     CDS             complement(6358..>7376)
                     /locus_tag="PLG72_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ80639.1"
                     /translation="RGSPVNFEYTLTRVKFNELTRDLVERTAVPVQNALTDAGIAASE
                     LSKVLLVGGSTRIPAVQEKVRALTGIIPSKSINPDECVALGAAIQADTLSGGSLMLYG
                     KDNILLLDVTPLSLSIETFGGVATRLVERNTTLPVRCSHIFTTVAPYQRSVEINVLQG
                     ERPLAKDNRSIGRFRLDGIKRAPAGVPQIEVTFDIDTNGILKVSARDLDTGREQSITI
                     TGNERMSDAEIEQAIRDAERYAAQDGIRRQTIELKAEGNRCLAAAQAALDKAKKEIPK
                     EEKKRIRTDIARLQKLIIKARPDRMTESDISALREAIAELRSNSANACRIAGVDQQNM
                     HHIQ"
BASE COUNT         1716 a         1933 c         1732 g         1995 t
ORIGIN      
        1 caagcttcag cacagtatcc tcacagaaaa tttcaagggt cgtgcagtca tcctcgttgc
       61 cgaaagtagc cggcagcccg tccagcttcg gcttgccgcc cgttatcctg tgggacacat
      121 actgcagctt gcttgccgca tggccgtcaa catcacggat ctcaaggcac gtttcgcgga
      181 aatccccgat cccgtgcgtc gggtactccc gcggaaaagc atcatagaat gacagcctgt
      241 ccctgttgtt tttcgatgga agaaacggat actcgtttgt atgcataagg ctttccggtg
      301 aaaaatgccc gtccccgata cgtcttccga aatatacatg cccgatgaaa tcctcatcat
      361 cgacgatccc gatgatatat gaagtgttgt tcgcgtcaag cttgaaaact ctctgatcct
      421 caaagtactt tattcccatt ccggtcctcc agttcccgca tcattggcaa atatccaggt
      481 atttttgatt atcataccag tctgctgccg atgatatgtt atagttaccg gataattata
      541 ccacaagttg cccctcatat cgaacacgac agaaatacgc aagcctccgt ataccggcat
      601 cctgctgctg ttgcgccaga tcccggaacc agacctgata attgaaaaat ttttgtatct
      661 tttgcagcat tttctccgtc taaagagtat acagagcgga ggggttgata tatggcatac
      721 aggtcctgtc ggccggagga cttcgatgca tttgtggcaa ggaatgaaaa cagattgttc
      781 cgcgctgctc tgtcaatgac gggaaatgcg tccgacgccg aggatatact gcaggaagta
      841 tttttgcggg catatgagaa agcccctgag tttacatcag gcgagcatga aaaagcatgg
      901 ctcatgaagg tcacgatcaa cctttgcaaa agccggcttc gttccccatg gcacaaacgc
      961 cgggtcgcca tgctggaatc ataccccgca accgaaccgg aacagcatga actaatggag
     1021 caggtcatgt ccctgccttc caaataccgc gcagtcatct acctatacta ttatgaggga
     1081 tactccgtca aagatatttc ggaactaacc gggcaaaggg agtccacagt aaggagccat
     1141 ctgacccgcg cccgtcagag gctcagatcg tttctgaagg aggaagttat gaaagcatat
     1201 aaagattata tggataaaat aacagtatca gatgcgcttc accggaaact cgtgtcacat
     1261 gcatcaggca acgtcaggcc tgttttgatc aggcggtatg cggcagcctt cgcctgcctt
     1321 gcattggcgc tggtctgtac ttatgcagtg acacagttat atcaaaacaa tacgccgcaa
     1381 gagccaggta aagatccata tgtcacaccc ggtgaaaccc cggtcgctgc aaagtccggc
     1441 gggtctcaga gtattgcggg ccacagtaat actgcgtccg atgaatccga tacaaacccg
     1501 tctgtttcac aatcagatga tacttcatct gcaggacagc cggaaggctc tccatcagta
     1561 tcaaaacctg atgaagctcc gtcagttccg tttcaggatg agagtccgtc cgtggccgat
     1621 atactagtca aacgcatctc ggactccgcc ggaaccgatg tgctgctaaa taatctaagc
     1681 ctcgatgaag cccgcgctga cgctgatttc ggtgcctatc tgccgaaaac cgttccctcc
     1741 ggattcgcct tcgaatccgc gacgcgctat gtcgatgaga aaagcaacca cctgaccgca
     1801 ttgtggttca aaggcatgcg catgatacac tggagcgttt ctaagcttga tgtggaggac
     1861 aaggccagga tcacctccgt atcggacaca aaaaactatg accttgcgct gtatcccatc
     1921 ccccatgcgg aatcagtacc ggaagattta agagaaatcg tggacgaccc catcttcatc
     1981 atagacgatc tgacgctgga cgcagtgaaa gcacgtgcgt atgaggtcca ggatgccggc
     2041 gatatctccg ggccccggat gagtttcagc gtgctgtatg gcgatacgct gataaagctc
     2101 tacgtgaagg gagcatcacc cgaatctgtt tttgacatgc ttatgcagat caaagcctga
     2161 cacggaacat gtagaaaatc gcttgtgact gtgcaatttt ttcgtttatg ggcgctttta
     2221 cggttgtttt tattgtatat tatccatatt ttagctacat aatatggcaa ttctcgtatt
     2281 acgtatactt agccgcgccc ataaacgaaa tttctgcaca ccagcaacag atcaggaaat
     2341 tcatctgcgg aaacgataaa agagatgtcc tgttgaggct taatatgatt tgagcctcaa
     2401 cagaagcgat tccaggaaat ccgcagtcat ggcatactgc agaaaacaaa agatttttgc
     2461 atcatccgca aacggatcag gcttctttaa tatcaaagca aaaagaatcc aacccgtttg
     2521 tgatggaccg gtcagcccgg aattttgaaa taagcgcttt cactcagctt atgcatctca
     2581 gagtccattt caaaggaaac gaagaagtcg accctgtatg tttcgcccgg tgcgaaatcc
     2641 ctggctgaaa gatcaagttt ttcgttcact gtgaattttc ccccgctgtt tattgtctcg
     2701 tcgactatag gaagtatgga aaacgggtat tcttcagaat gcctgtatac ttcatttccg
     2761 ctttcgtcat atacggcaaa atcgtaaaac ggatagtaca aagtaattgt aatgtccttg
     2821 ccggcattat ttcttacctg gaactccagg tcgatggtat tgccggcggc atttgctatc
     2881 aatgccgtgc tgacggcgtc actttccgcg taggaaccat cggtcttttt atccgaaata
     2941 ttgactgtcc ttgtcgcctg gtcccatgtt acttctttgc cgaacagttc tgcagctttc
     3001 ctcagtggca catatgcggt attgttatac acaaaatggt cgagatcggt tttttctccg
     3061 ttgtacaaaa tgttaaggga gttgagcatg acctggatac tcgaacttgc gaatgcatgc
     3121 gcagctgcta atgctatgca taccgttact gctaataata gcgacaatct tttagtggtt
     3181 cgtgacatgt atatccctcc gtaatctgat tttttccgcg gctgtttcct ttatcagccg
     3241 tcatatgaaa gacgtttctg ccgttcaaaa tgttgcacgt cccggcacaa aaagaagccg
     3301 gttgaaatac aaccggcttt tggccaatga atgtccccgc aaaatcactc ggtccgcaaa
     3361 gcaactacag gatctttctt agctgcgctt cgggacggta tgatccctgc gatcagtgtc
     3421 aggagcatgc tgattacgac aagcataacc gcgacctcag gcggaaggaa agctgaaagg
     3481 ttgctgagac ctgtcagatg atggagtatc atgttgatcg gtatgcacag caagtatgtg
     3541 atcagtacgc ccagcaggcc ggaagtgaat cctattataa cggtctcggc attgaacagg
     3601 cttgatacat ttcccttcga tgcgccgatg gcacggagga taccgatctc cttggtgcgc
     3661 tcctgcactg aaataagcgt gatcacgccg atcattatag atgatacgat aagcgacact
     3721 gcaacgaacg ctataaggac ataggtgatc gcgtttatga tcgttgtgac cgacgacatt
     3781 atcagaccta cataatcggt atatttgatt tccttcaaat catccacgct ttcattgtac
     3841 tctgcaatga cttcttctat cacgtccttg ttggcaaatg acgacgcata caggttgata
     3901 gcagcaggac tgccgcggtc gacaaggccg agcctgagca aattatcctc ataggtggaa
     3961 tccgagaata ccaccagttc atcataatag gcagcacact gttcatcggt gaactgttcc
     4021 aatgtcatat cgagcgctgc cgcaagctgc tcgccgctca tggcgctcat ctgttttgca
     4081 gcctcagcgg catactgcgc cctgaactgc tcttccagcc ccttgacaag cagttccgaa
     4141 agatcttcat cgctcattga agcaatatag ccggcaatcg tttcctcatc cattcccgtt
     4201 tgctcagcca ttgccggagc gatcgccttt tccatgtcag cgcgcgtcat acctgccagc
     4261 gtgcctgaaa caaactcctg taccgcttcc tccgaaggaa tgctcatgat ctttttatat
     4321 gcctccgctt tcccggcagt atcaagggat gagacgtaat tcctgaattc gtcagccctg
     4381 ctgctttcag tctgatcccc gctggtgctc ctgaacggca ggccggtgaa aatgtcggtg
     4441 gacggatccg caagctgtgc ttttacggca tcagactcat tcgctttatc gatgatgtaa
     4501 tcagtgagca tatacgtata acctatggac gcgttcgtca tcggtgagat agcgtcttca
     4561 tccggctttg caataccgac gacgcggagc ttcagcgcat tgtcgtacag atacttaagt
     4621 cctgcttccg tttcccgcag atccgtatat gtccctgtct tttcatcata cgtatagcaa
     4681 tcgcagttaa ggatggtgcg gaaatccata ttgcatatat cttcatacga ccatttcctt
     4741 atctcatagt caactgtctt cttgttcaga gcggcgtcca taaggatctc aatttctttc
     4801 tcagacaaca gtcccagcgt gtaaagcgtc agatcgtcga tctcattgtt ctcatccaca
     4861 aaaagtacta gttcgtcata cctggacggc catcttccgt agattatgtc atattgcttt
     4921 tcagtaacgc tgttgacagg tttccccgaa tctcccggca caagttcctt ccagaggccc
     4981 atggactgct tcatcaggga atccgtacgg cccatgaccg tttgctccct cagggcaagc
     5041 atcgtcgaca tatccatttt ggcgtatttt ttcataactt cagtcagtat cgtctgcctg
     5101 tcggaacgaa tgatgcttcc atccacgttt ttcgtatata tcatcaggtc aagatcataa
     5161 gaatactgta cgcctgacag cgcggaatgg agcttgctgt tctcatccgc acgctgtgcc
     5221 tcgatatact ccttgaatgc cttaaggtcg tttttctgcg tatcgagcga gttcatcgaa
     5281 ttcatcatct cgtatatcat tgctttctga tatacggcat cgtcttcatg ttcggaaacc
     5341 gacttcgctt tccccaggaa agtggagatc aggctcgaca tatccacgct ctgcgcctcg
     5401 atcgtcagcg ggtaggaagc cagcgtatct tcctggaccg aatcgatgaa agccgtcgtt
     5461 ccttttgata cggcataaat cagcgctatg ccgataatgc ctatggaacc ggcaaacgat
     5521 gtcagagcgg tcctgccttt cttcgtgaaa aggtttttca gcgacaggcc gaaggatgtc
     5581 aggaacgaca tcgagggcat tttcttttta tgcgtttctg ttttcttttt ctttgcctca
     5641 tcaagttcct tcctgtattc ttcatccgtg agcggctggc tgtcgtcgat gatcctgccg
     5701 tcaagcattt ttacgatacg ggtcgagtat ctctccgcaa ggaccgggtt gtgcgtgacc
     5761 ataatgacga gccggtccct ggctatttct ttcaggatat ccatcacttg tctgctcgtt
     5821 tccgtatcca gcgccccggt cggctcatct gccatgatta tatccgggtt gttgacgata
     5881 gcgcgtgcga tcgcaacgcg ctgcatctgt ccgccggaca gttgggcggg cttcttcctc
     5941 aactggtctc ccagaccgac tttctcaagc gcttctctcg cccgctggcg gcgttctttt
     6001 ttgggtacgc ccgtgagcgt cagcgcgatc tccacgtttg acagcacggt ctggtgcggt
     6061 atcaggttat agctctggaa cacaaaaccg acagagtggt tgcggtaagc gtcccagtcg
     6121 cggtctttat attccttggt cgacctcccg ttgataataa gatcgcctga agtataactg
     6181 tcgaggccgc cgatgatgtt cagcagggtc gtcttgccgc agccggacgg cccgagtatg
     6241 catacgaatt cgctgttgcg gaactgcagg tctatccctt tcagtgcttc tacatcttcg
     6301 cctgcggtat ggtaagtttt ggtgatgttt ttcagttcaa gcattctcac tactccttta
     6361 ctggatatgg tgcatatttt gctggtcaac acccgctatc cggcaagcat tggcactgtt
     6421 cgatctcagt tcggcgatcg cttcccggag ggctgaaata tcgctttcag tcatcctgtc
     6481 cggccttgcc tttattatga gtttttgcag ccgggcaata tcagttctta tccttttttt
     6541 ctcttccttc ggtatctctt ttttggcctt gtcaagcgcg gcctgggctg cagccaggca
     6601 cctgttcccc tctgctttga gttctatcgt ctggcggcgg ataccatcct gcgccgcata
     6661 tcgttcggca tcccgtatcg cctgttctat ttctgcatct gacatgcgct cattgccggt
     6721 tatggtgatc gattgctccc tgccggtatc gaggtccctt gcggatactt tcagtatgcc
     6781 gttcgtatct atatcaaagg taacttctat ttgcggcact cctgccggag cacgctttat
     6841 tccgtcaagc ctgaatcttc cgatactcct gttatccttc gccaatgggc gctcgccctg
     6901 caatacattt atctcgacac ttcgctggta aggcgcaaca gtggtgaaaa tatgggaaca
     6961 gcgcaccggg agcgtagtgt tgcgttccac gagccttgtg gctacaccac cgaacgtttc
     7021 aatggaaagg ctcagcggcg tgacgtcaag cagaaggata ttgtctttcc cgtaaagcat
     7081 cagtgacccg ccggaaagcg tatcggcctg tatcgctgcg ccaagcgcga cacattcgtc
     7141 ggggttgatg ctctttgatg gaattatccc tgtcagcgcc cttacttttt cctgtacggc
     7201 cgggatcctg gtcgaaccgc cgactagcag cacttttgaa agctccgacg cggcaatgcc
     7261 ggcgtcagtc agcgcatttt gcaccggaac ggcggtgcgt tcgaccaggt cacgcgtcag
     7321 ttcattgaac tttacgcggg taagggtata ttcaaaattg accgggctgc cccggt
//
LOCUS       DAOSCC010000119         7367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6481_length_7367_cov_5.666028, whole genome shotgun sequence.
ACCESSION   DAOSCC010000119 DAOSCC010000000
VERSION     DAOSCC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6481_length_7367_cov_5.666028"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="PLG72_07305"
     CDS             <1..97
                     /locus_tag="PLG72_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80640.1"
                     /translation="ASVIYMVPMLLIFLYGEKYIVEGIQLSGLKA"
     gene            134..1396
                     /locus_tag="PLG72_07310"
     CDS             134..1396
                     /locus_tag="PLG72_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ80641.1"
                     /translation="MDIRTIEENMNLLKQKRIRKAIIVFFLFMLIMTFLSKTLSNLSL
                     PRVNAVKPSAGYLVREIRGDGVIEPVEAYEIYPDKNVKVKEILVKSGSYVSKGEKIAE
                     TDITEAEELYAKSMLRYENAKLDYQKLLLSKNGLPELEKEIERIEGEIGEKQAELEDI
                     RQLYSVGAQSADDVSAAEKSLKNARDDLAQKRQEYEKRREQYEIDIRKAQNELKIMEM
                     ELEDMKVEEGIYAPADGIIKTVNDKRDVEKGAPVCVLVDPSKGFRLRIPASKTKTGYL
                     SIGEMLKVDIRNADIQVDGTIADIRDNVTDPANKKDLIIVIESDSLSGGETGSVRFAK
                     KSGVYSLLVPNSAIHEDSEGTYVFVVDEKQGFLGNENYVRKAYVEIEDYDDSKTAVSS
                     GITIDSLVMTGSSKPVFNGDVVRLAVTR"
     gene            1420..2781
                     /locus_tag="PLG72_07315"
     CDS             1420..2781
                     /locus_tag="PLG72_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80642.1"
                     /translation="MNRKTAFLYTFIFLFLFVSAYVLCDISSNQHVPEYLANSVNLFS
                     IAVAEDTGRSGTYISEAEYESLDSLIDKLSMCCHAFSHTGITAMRKMHNANIIFTDYR
                     YAGMNGMEFAYGNYFTEENQSGGDLKAVIDRKTAWELFGTTNDATGLTIGIFGREFEI
                     IGVAECDAGLIQYINDDEINVYIPISVLKLSGEDTKITGILVNFPDDVSSYEGKDRIA
                     RALEAIGKSSYGYRIKSLRTTAVMIRMFSNLFICIIAIITAVIIIRHLYNHFGESIEK
                     SRNDMAASGYKAAFKRFFTGQRILLPAVYLVLIVFIYLSVRSGAKAFLDFYDKDPGIA
                     SVYEHFIECFSQGMHAGSVAHWTSERLFILSSCSLLTGVFAGLPVLLSGMSFLKKFAE
                     DRHTLLIYSGIAFLSITLLVIVSCTLAGLEPSVRIPSILVIWILFTVILADRKGSVSS
                     TNE"
     gene            2979..3452
                     /locus_tag="PLG72_07320"
     CDS             2979..3452
                     /locus_tag="PLG72_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ80643.1"
                     /translation="MKRTISWAAVLSWAAVLLWMALIFWFSSQPADQSNQMSTGITEI
                     IARAIERIIPGTEVIIGELNHIVRKNAHFISYLVLGILNAAALRNSGAEIKKAFLISF
                     VICAVYAVSDEIHQLFVPGRGCQFTDVLIDSAGALVGSGIVCAAGAIRRKRQASE"
     gene            3610..4407
                     /locus_tag="PLG72_07325"
     CDS             3610..4407
                     /locus_tag="PLG72_07325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ80644.1"
                     /translation="MNIGFGFYPGGKRKALTMSYDDGQVHDRKLVEIFNRYGIRGTFH
                     LNSGRFDIDGFLKSSEIRDLFKGHEISAHTLNHPYLTILPGDMVATEVLEDRKNLEEL
                     AGYPVRGMSYPYGAYSQELTEILPFLGIEYCRTVNSHNGFQLPDNFLSWHPTCHHKQG
                     LMEKAKAFKNIGKWAQMPLLYVWGHSFEFEWDKNWDLIEEFCKTVSGDDDVWYATNIE
                     IADYVRAMKSLKFSVDRKTVHNPSALDVWISIDGEPCRIEAGKTVKL"
     gene            complement(4642..5715)
                     /locus_tag="PLG72_07330"
     CDS             complement(4642..5715)
                     /locus_tag="PLG72_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ80645.1"
                     /translation="MQLRTDYTYALVMPTSMGVRITPLDRRPVHTSNLYIMQATSAES
                     NVANIAASLGMRVKLLTTFVKDSEIALFIKSELRKRNIEFEGPEVPQGGPWGYRHQFN
                     IADSGYGMRGPRVLNDRAGEVGRTLNSKDFDIDRIFGKEGVAILHLSGLIAALSRDTS
                     QFCLELAKAAKKHGTKVSFDLNYRASFWAGREEELRDTFSRIAALSDILIGNEEDFQL
                     ALGIEGPEAGGSDLDKKIDSFRHMIENAGKIYPNVSVFATTLRQVLNANSHLWGCIML
                     YDNKWHVIEPREIPVYDRIGGGDGFVGGLLYAILKGWEPEKWPQFGWATGALVVTVPE
                     DYGQPVDEAQVWSIYKGNARVKR"
     gene            6045..6902
                     /locus_tag="PLG72_07335"
     CDS             6045..6902
                     /locus_tag="PLG72_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ80646.1"
                     /translation="MDLFSLEGRKAALLGGGGVLGAAMAKGLVDAGADVAVCDLDLEK
                     AQAVVDSVTSGKSGGITCEDKPRRPVVKAYRVDAMDIESIRATAAAIFNDFGRVDILV
                     NAVGGNMKGATTSDEVSFFDLDAEMLNKVVKLNLMAGAVLPSQVFGREMVRNKNGGVI
                     INISSMNAFRPLTRIPGYSAAKAAVSNFTQWLATDLAKKYGDKIRVNAIAPGFFLTEQ
                     NRFLLTDPETGEFTRRGKTIIEHTPMGRLGRPEDLIGTLIWLCSDASRFVTGIVVPVD
                     GGFSAFSGV"
     gene            7031..>7367
                     /locus_tag="PLG72_07340"
     CDS             7031..>7367
                     /locus_tag="PLG72_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOJ80647.1"
                     /translation="MEKQDIGVIGLAVMGKNLAMNIESKGYSVSVYNRSREKTDEMMD
                     EVKGRRIHGTYSLEEFVNSLKTPRKIIIMVKAGQPVDDMIQQLKQYISKGDIIIDAGN
                     SFFGDTIRRS"
BASE COUNT         2041 a         1569 c         1985 g         1772 t
ORIGIN      
        1 ggcatccgtg atttacatgg tcccgatgct gctgatattc ctgtatggcg aaaaatatat
       61 cgtcgaaggc atacaactgt cagggctgaa ggcatgaaaa cgcatatttc ccggatacgg
      121 aaggagattt gaaatggata taaggacaat agaagaaaat atgaacctgc tgaagcaaaa
      181 aaggatcagg aaagccataa tagtgttttt cctgtttatg ctcatcatga cctttttatc
      241 aaaaacactg agcaaccttt cactcccgcg ggtgaatgct gtgaagccct ccgccgggta
      301 cctggtaagg gagataaggg gcgatggcgt cattgagccg gtcgaggcgt acgagatcta
      361 tccggataaa aacgtaaagg tcaaggaaat ccttgtgaag tcgggaagtt atgtgtctaa
      421 aggcgagaaa atagcggaaa ctgatattac agaggctgaa gaactgtatg ccaaatcgat
      481 gctgagatat gagaatgcga aactggatta tcagaagctg ctgctcagca aaaatggatt
      541 gcctgaactg gaaaaagaga tagaaaggat cgagggtgag atcggggaga aacaggcaga
      601 actcgaggat atcaggcaac tgtacagtgt aggcgcacaa tcagctgacg atgtttcagc
      661 tgcagagaag tctttgaaaa atgcccgcga tgacctggct caaaaacggc aggaatacga
      721 aaaacgcagg gagcaatatg aaatcgatat ccggaaagcg cagaacgaac ttaaaataat
      781 ggaaatggaa ctggaagaca tgaaggtgga agagggtatc tatgcacctg ccgatggtat
      841 cataaaaacg gtcaatgata aaagggacgt tgaaaaagga gctcctgtct gcgtgctggt
      901 cgatccctca aagggattca ggctcaggat cccggcaagc aaaacgaaga ccggttattt
      961 gagcattgga gaaatgctta aagttgatat aaggaacgcg gatatacagg ttgacgggac
     1021 cattgccgat ataagggaca atgtcaccga tcctgcgaat aaaaaagatt taataatcgt
     1081 gatcgaaagc gacagcctga gcggcgggga gacagggtcc gtaaggttcg cgaaaaaaag
     1141 cggtgtatat agtttattgg taccgaattc cgccattcat gaggattcgg aagggacata
     1201 cgtgtttgtg gtcgacgaaa agcaggggtt tttgggtaat gagaactatg taaggaaagc
     1261 atatgtcgaa atcgaagatt atgatgactc aaagacagca gtaagttccg gtatcacgat
     1321 cgacagcctt gttatgaccg gaagcagcaa gccggtcttt aacggagacg tggtcaggct
     1381 ggcagttacc agatgaaaaa taacggtcgg ggtggacatg tgaacagaaa aacagctttt
     1441 ctctacacat tcatatttct tttcttattt gtgtcggcat acgttttgtg tgacatttca
     1501 tcaaaccagc atgtgccgga ataccttgca aacagcgtaa acctgttcag catcgctgtt
     1561 gccgaagata cggggcgttc aggcacatac atcagcgaag ccgaatatga atcgctcgac
     1621 agcctgatcg acaagctttc catgtgctgc catgctttca gccataccgg gataacagcg
     1681 atgagaaaga tgcacaatgc aaatataata ttcactgatt acaggtatgc cggcatgaac
     1741 gggatggaat ttgcttacgg gaactacttc actgaggaaa accagtccgg cggagacctg
     1801 aaagccgtga tagacaggaa aacggcatgg gaactctttg gtacgactaa tgatgcaacc
     1861 ggcctgacca tcggaatttt cggcagggag tttgaaatta tcggagtagc tgagtgtgac
     1921 gccggtctta tccagtatat aaatgatgat gagatcaatg tttatatacc catatctgtg
     1981 ctgaagctct caggggaaga tacgaagatc acgggcattt tagtcaattt ccctgacgat
     2041 gtgtcatcat atgaaggcaa agaccgtatt gccagggcct tggaggcaat tgggaaaagt
     2101 tcatacggat acaggataaa aagtctgcgg accacggcag ttatgatacg gatgttcagt
     2161 aatttattca tatgtattat tgcaattatt actgctgtaa taataatcag gcatttgtat
     2221 aaccatttcg gcgaaagtat tgagaaatcc aggaatgata tggctgcttc cggatataaa
     2281 gcagcattta aaaggttttt tacaggccaa agaattctat tgcctgcggt ttacctggta
     2341 ttgatcgtgt tcatttactt gtcggtaaga tccggggcaa aggcattcct ggacttttat
     2401 gataaagatc ccggtattgc ttcggtatat gagcatttca tcgaatgttt ttcgcagggc
     2461 atgcatgcag gcagcgtagc gcactggact tctgaaaggc tgtttatact ttcatcatgc
     2521 tcattgctga caggcgtatt tgccggattg cctgtcctgc tgtccggtat gagtttcctg
     2581 aaaaagtttg cagaagacag gcatacactg ctgatttact ccggaatcgc gtttttgtcg
     2641 ataactctgc ttgtgatcgt ttcctgcaca cttgccggtt tggaaccgtc agtaaggatc
     2701 ccatcgattt tagtgatctg gattttattt accgtcatac tggcagaccg gaaaggctcc
     2761 gtatcatcca caaatgaata atgaaacagt tcctgataaa cggataccga aattgttgca
     2821 atatctgcat aatccgggtg gatgaaggct gtttagaata aggagatcct gataattata
     2881 ccggttttga tgatgtaata tgaaaccggc tgtttttttt atgctataat gtctctggac
     2941 tctacattca caagaaaggg ctaaaccagg taaaccacat gaaacggacc atatcatggg
     3001 cagcagtact atcatgggcg gctgtgctgt tgtggatggc attgatattc tggttttcgt
     3061 cgcagccggc ggaccagtcc aaccagatga gcacgggaat aacggaaatc atagcccgtg
     3121 caattgagag gataataccc ggcacagaag tgatcatagg cgaactgaac cacatcgtgc
     3181 gcaaaaatgc gcacttcata tcataccttg tacttgggat attgaatgcg gctgccctga
     3241 ggaacagcgg agcagagata aagaaggcat tcctgatatc gtttgtaata tgcgctgtat
     3301 acgccgtttc cgatgagata caccagcttt ttgttccagg caggggatgc cagttcaccg
     3361 atgtgcttat cgacagtgcc ggggcgctgg tcggatcagg gatcgtgtgc gctgcagggg
     3421 ctatccgaag gaagcggcag gcaagcgaat gacaggcggg aaagcggaga caggcacgaa
     3481 aaaactgaaa atgtgaaatc gccgggacac gggaaaatcc ggaggcgttg gatttgtggg
     3541 gggaccggaa aagcgcaggg caggacatgc aatgaccata aacaaatcaa cacaggggag
     3601 gttattttta tgaacatagg ttttggcttt tatcccgggg gaaagagaaa agcgctgacc
     3661 atgagctacg acgacggaca ggtgcacgac aggaaacttg ttgagatttt caacaggtac
     3721 ggcatcaggg gcacattcca cctgaactcg ggcaggttcg acatcgacgg ttttttgaag
     3781 tcatctgaaa tcagggacct gttcaagggc catgagatat cggcgcatac gcttaaccac
     3841 ccgtacctga cgatattgcc cggcgacatg gtggcaacgg aggtgcttga agacagaaag
     3901 aaccttgagg aactggccgg ctatcctgtg cgcgggatgt catatccgta cggggcatac
     3961 agccaggaac ttactgaaat acttcctttt ctcggcatag agtattgcag gaccgtaaat
     4021 tcccataacg gcttccagct gccggacaac tttctttcat ggcatcctac atgccatcac
     4081 aagcagggcc tgatggaaaa agccaaagca ttcaaaaata taggcaagtg ggcccaaatg
     4141 cccctgctgt atgtctgggg ccacagtttt gaattcgaat gggataaaaa ctgggatctc
     4201 attgaagagt tctgcaaaac ggtgtccggt gacgatgacg tatggtatgc gacaaacatc
     4261 gagatcgcgg attatgtcag ggcaatgaaa agcctgaagt tcagcgtcga caggaaaacc
     4321 gtgcacaatc cgtcggctct ggacgtatgg ataagcatag acggcgagcc gtgcaggata
     4381 gaagcgggaa agacagtgaa gctttgattt gagacacttg atctggaaga catttataaa
     4441 agcagccggc taatctgtaa gaacctgcat tcgggaatta cacagcctta aactttacct
     4501 gaaaaaactt ccggaagttt tagcttccgg aagtttttcg gataattcga ctgatgcttt
     4561 tagatcgcct gatttaaaag aggtatttga aacctgtgac tgatattgaa gatttgattt
     4621 ctgaggattt ggtccaaaca gttacctttt aacccgggca tttcctttgt agatgctcca
     4681 gacctgcgct tcgtcgacag gctggccata gtcttcggga actgttacga ccagcgcgcc
     4741 tgttgcccag ccgaactgcg gccatttttc cggctcccat cccttcagta tagcatacag
     4801 caggccgccc acaaatccgt cgccgccgcc tatgcggtcg taaacgggga tctcccgcgg
     4861 ttctatcaca tgccacttgt tgtcatacag cattatgcag ccccacagat gtgaatttgc
     4921 attcagcacc tgccgcagtg tggtggcaaa taccgacaca ttggggtata tctttccggc
     4981 attttcgatc atgtgcctga aactgtctat tttcttatcg aggtccgacc cgcctgcctc
     5041 cgggccttcg atgccaagtg caagctggaa gtcttcttcg ttgcctataa gtatatctga
     5101 aagggcggct atccgggaga aagtatcccg aagctcttct tcacggcctg cccagaacga
     5161 agctctgtaa ttgaggtcga agctcacctt ggtgccgtgc ttttttgccg ccttcgcaag
     5221 ttcaaggcag aactggctcg tatcgcggga caatgcggct atcaggccgg aaagatgcag
     5281 tattgcaacg ccttctttcc caaatatgcg gtcgatgtcg aaatcttttg agttcagcgt
     5341 ccttccaact tctcctgccc ggtcgttgag cacgcgcgga cctctcatgc cataaccgct
     5401 gtcggcgatg ttgaactggt gcctgtaacc ccagggcccg ccctggggca cttcaggacc
     5461 ttcaaattcg atgttgcgct tgcgcagttc gctttttatg aaaagggcta tttcgctgtc
     5521 ctttacgaat gtggtcagca gcttgaccct catgccaaga gacgctgcaa tattggccac
     5581 gtttgattcg gcgcttgtag cctgcatgat gtacaggttg ctggtatgga ccggccgacg
     5641 gtcgagagga gttatcctta cgcccatact ggtaggcatc acaagcgcgt aggtataatc
     5701 ggttcgtaat tgcatttttc aaacctcccg gcaaatattt cactatgcag aagcaaaaat
     5761 gatttgtgtg agtgcataaa tgcagtgaaa atgtttttat ctagatacat cattatagta
     5821 atatagtaac aggcagtttt caatgaatat gctgcctttg agcaatattt acatattaac
     5881 aggcaataat agcaaggatt aactccgccc gttgatttat aatggtaata ggcagaaata
     5941 tgcgcatttg atgagattat cgggtcggac cggtgatggt cggaccggtc gtcacgagca
     6001 aatggccgac tgttatatat aaaatgaagg cgaggaggta gaacatggat ctgttcagtc
     6061 tggaaggaag gaaagctgca ctgcttggag gcggtggagt tcttggcgcc gccatggcga
     6121 aagggcttgt cgatgcaggc gccgacgtgg cagtttgtga ccttgacctg gaaaaggccc
     6181 aggctgtcgt tgattcagtc acttcgggaa agtcaggcgg aataacctgc gaagataagc
     6241 cacggagacc tgtggtaaaa gcatacaggg tggacgccat ggatatcgaa agcatcaggg
     6301 cgacggcagc agccattttc aacgatttcg gaagggtcga catactcgtc aatgcggtgg
     6361 gcggtaatat gaaaggcgcc accacgtcgg acgaggtatc ctttttcgac ctggatgctg
     6421 aaatgctcaa caaggtggtc aaactgaacc ttatggcagg cgcggtgctt ccgtcccagg
     6481 tatttggcag ggagatggtc aggaataaga acggaggcgt gataatcaac atatcctcga
     6541 tgaacgcatt caggccgttg acacgcatac cgggctactc ggctgcaaag gcggcagtga
     6601 gcaatttcac ccagtggctg gccaccgatc tggcaaaaaa gtacggcgat aagatcaggg
     6661 tcaacgctat tgcgccgggc ttcttcctga ccgagcaaaa caggtttttg ctgacggatc
     6721 cggaaacggg ggagttcacg cggagaggga agaccatcat cgagcatacg ccgatgggca
     6781 ggctgggccg gccggaggat ctcataggga cgctcatctg gctctgttcg gacgcgtcca
     6841 ggttcgtgac cggcatagtg gtgcctgtgg atggcggatt ctcggcattt tcaggcgtat
     6901 gacgccgttg ttgccggaac agcgacctgc ctcggaggcc ataccgttca aggcagtatc
     6961 agccggcaag tattttcgga tgattccgga tgacataaag taaaaatgtt gcaaaaggag
     7021 tgatcgatcc atggaaaagc aggatatagg agttattggc ctggccgtta tgggcaagaa
     7081 ccttgcaatg aacatagaga gcaagggcta ttcggtctcg gtatacaaca ggtcaaggga
     7141 aaagaccgac gaaatgatgg atgaagtgaa gggcagaagg atccacggga cttacagcct
     7201 tgaggagttc gtgaactcgc tgaagactcc gaggaagatc atcataatgg tcaaggccgg
     7261 tcagcctgtg gatgatatga tccagcaact gaagcagtat atttcgaagg gtgacatcat
     7321 aatcgatgca ggcaattcct tcttcggtga tacgataagg aggagca
//
LOCUS       DAOSCC010000120         7289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6555_length_7289_cov_4.800249, whole genome shotgun sequence.
ACCESSION   DAOSCC010000120 DAOSCC010000000
VERSION     DAOSCC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_7289_cov_4.800249"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="rlmD"
                     /locus_tag="PLG72_07345"
     CDS             complement(<1..786)
                     /gene="rlmD"
                     /locus_tag="PLG72_07345"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOJ80648.1"
                     /translation="MNKNDIYEIEITGMTHEALGVGKVGDMVVFVQGAIEGEKVTAKI
                     IKVAKNYAVARVEEWITKSHGRQEPFCPVYKRCGGCSLQHMTYDMQLKFKHRVVTDNL
                     ERIGGFRGIRVNQVIGMVDPMNYRNKAQYPVGIGDSGSIAGFYARRSHTIIDSESCGI
                     QHHGSEKVRSAVMDAVRELKIPAYDEETGDGILRHIVTRVSYSTGDIMVILVVTKENV
                     PGLDRLIRKLTCEVPEVKSIVLNINKRRDNVILGDTVRTVYGGD"
     gene            complement(1207..3789)
                     /locus_tag="PLG72_07350"
     CDS             complement(1207..3789)
                     /locus_tag="PLG72_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ80649.1"
                     /translation="MMIKIRAKLILAFAATILICSAATLAVTLGGYNMMVSEIAASAD
                     SNNSRIISVREISGMVHKQYQALSSAVVGRDALPADAVEAENSKIREAVDRLASQSED
                     SERAELENLKNLADQFAQLCKTDISKGIEKSDPTEYNALLADFSLQYDSLIQKETELK
                     SVILKQIDAAAAKLSKDAAGIGSLSAEQIAALDELLPAAEKVLQEYKSAAEANGSLLL
                     LKDKQQIRINELETNIDALLEEISRLKTELEVLKAQQPAGYTGRSSAGTAYSAGGTQP
                     ADTSAAPVSVELPAAGPTGTQPGSSSPSGISPAVYDQVLADSVRTYLDAVLASGTDIR
                     NIVSGLSAAGLQEALQRLSSVNAALTLTAEAYGRAQAGLAVAGSPETESALNAIKSAA
                     AELEKLGSELNSEHAALAAEAAAQCSRMEEAIGSLLEAKKGLEDNGLSESFAGASDLY
                     DRQIELLSKLETSYKSYLADDLERSQELKNNLLITLAVISFISLFIGMFAALWLSGNI
                     LGPIRNMTNLLDKASKGDLSGRVRDRRRDELGELGEKVNVVLDGQQKMLEQVRTTSTS
                     IGKLRNSLVELFSHSRENTGKVSKGFRSIMDGLMAVIKTSGTETAAAADDTDIIAATA
                     GKAVEDGIKAIEIAANGERSVQEAEEVIRNVTDAVKQIAASINELEDSSGKIGNITDT
                     ITEIASKTNLLALNAAIEAARAGQQGKGFTVLAEEIRKLSERSNAAAKEIRHLISEIQ
                     ERIQYAVGRIGDGAASVDVGVGKINHARNSILEMTGVIRQIVETLKATANAISSGQES
                     TAELAGTISTIEKAANETAASGEELDAELELHQKTLKEMEAVTEKLDEVTDALNELLE
                     RFRI"
     gene            4148..5086
                     /locus_tag="PLG72_07355"
     CDS             4148..5086
                     /locus_tag="PLG72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ80650.1"
                     /translation="MKKGKAKRAQKKYLSIVLVPHSSSRVKVLRFNLFYAKLVTFFVV
                     LIAVFVFGGLYISKLLDENNMLKQNVSNLYSTTAEQSTLLQKKSEEIESLKRESEAFR
                     EVVNDRIEEFTEKFNQLTDEYLEEQSARVSRSGERTETAFASDMRELKTSLDKLIQLY
                     SRSKRPDADIEAVEEKINAFMEVIPTLWPTEGRITDQFGYRKDPFTRRKSYHSGLDIA
                     ADVGTSIKAAASGKVIYADYTYATGRTVKIDHGNGFVTVYGHCSKILAEPGQQVKKGE
                     VIAKVGSTGRSTGPHCHFEIHLFGTAIDPLEYLEKK"
     gene            5170..5619
                     /locus_tag="PLG72_07360"
     CDS             5170..5619
                     /locus_tag="PLG72_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ80651.1"
                     /translation="MLKRKASFREGVDTLVGSNSVFKGNIESEGTVRVDGKVIGDIKV
                     EGDVYIGNGAAVEGNVDAGNVYLAGMIKGNIVAKGLLKILSTAKLYGDIKINSFVADE
                     GALFQGKCEMIDGTESEKPPEKHRRNYKKSTVLEDIYEDSHSAGTDA"
     gene            complement(5644..5802)
                     /locus_tag="PLG72_07365"
     CDS             complement(5644..5802)
                     /locus_tag="PLG72_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80652.1"
                     /translation="MIRNSNLYRRWWYIKRRNINRIRLLLRLLIALLIMGAVLLFAEL
                     YIDGFIAL"
     gene            6166..>7289
                     /locus_tag="PLG72_07370"
     CDS             6166..>7289
                     /locus_tag="PLG72_07370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOJ80653.1"
                     /translation="MKFEKKPSAGGAEKKHNKPKKADKPNRKKNSSFIKKFALTLIAC
                     ILLFIFAIGCALTGLVGGAVYAYIETAEPISDEQLIAKASSRTTLIYDAKGNIIHKLT
                     GKDNMDSEPVTDKEVPQYLKDAIISIEDERFFQHPGVDIQGIINAGLGFVKSIITGND
                     EGLRGGSTITQQVVKNITGNTTRSIKRKVQEAYAAILLERKLEKWQILELYMNLGYWG
                     NSCTGVQSASKKYFGKPVSELSLAQCALLAGITKNPAKYNPFTEKGREYAKERQEIIL
                     GKMLELGKISQEEYDQAIKEDLRYALNTGSQKTANVQSYFVDQVIVDVRNALMEEMNI
                     SSTMANYMIYGGGLEIYTTMDPKIQADMDSVFMDDTYFPLV"
BASE COUNT         1810 a         1906 c         1707 g         1866 t
ORIGIN      
        1 atcgccccca tacacggtcc tgacggtatc tccgagtatc acgttgtccc ttcttttgtt
       61 gatgttcaga actatgcttt ttacttcagg cacttcgcaa gtgagttttc ggatcagcct
      121 gtcaagaccg ggaacgtttt ctttcgttac cacgagtatg accatgatgt cccctgttga
      181 gtaagaaacc ctggtcacga tgtggcgcag gatcccatct cccgtttctt catcgtaagc
      241 aggtattttc agttccctga cagcgtccat gaccgcgctt ctcacttttt cgctgccgtg
      301 atgctgtatc ccgcagcttt cggaatcgat gatggtatgg ctgcgtcttg cgtaaaagcc
      361 tgcgatcgat ccgctgtcgc caatgccgac agggtactgg gccttgttcc tgtaattcat
      421 cggatccacc atcccgatga cctgatttac acgtatcccc ctgaacccgc ctatcctttc
      481 gagattgtcg gtcacgaccc tgtgcttgaa cttaagctgc atgtcatatg tcatatgctg
      541 aaggctgcag ccgccgcatc ttttgtatac cgggcagaac ggctcctgcc gcccatggct
      601 tttggttatc cattcttcca cccttgccac ggcatagttc tttgcgacct ttataatttt
      661 agcagtgact ttttcccctt ctatggctcc ctggacaaat accaccatgt cgccaacttt
      721 tccgacgccc agagcttcat gcgtcattcc tgtgatctct atttcgtata tatcattttt
      781 attcataaga tagcctctcc tcagtcatat gcatatcata tcacagcagg aagtccaact
      841 actgcataat tttaattata ttattggtac gtaaatgcgg aactattttt tcaaggctgg
      901 aacaacattc tgtgccgttt tacagcttct gggcagtcgt gcctccggtt tttggatgcc
      961 gattttcagg ttttaggcac tcacaatcgg aatccctgaa ttgcttgttg acataaagcg
     1021 tgtaaatcca accttatgta tgccattgct gtaataacaa agcccatcaa acagaaaatc
     1081 catcccagaa gttgaaaaaa agcacaataa tctcagcaat aacccataag aaatttatcg
     1141 cacagcatct ttgatttacc ggctgacagc tctccattcc ggaggttcct ggaccattcc
     1201 tggaaattat atcctgaacc tttccagcaa ctcgttgagc gcgtcggtga cttcgtcgag
     1261 tttttccgtc actgcttcca tttccttaag cgtcttctga tgcagttcca gttcagcatc
     1321 gagttcctca ccggatgcgg ccgtctcgtt tgccgctttc tcaatcgtac ttatcgtccc
     1381 ggccagttcc gccgtacttt cctgtccgga gcttattgca ttggccgtag ccttgagggt
     1441 ctcaactatc tggcgtatca cgcccgtcat ctcaaggatg ctgttccttg catggtttat
     1501 cttgccgacg cctacatcca cgctcgccgc tccgtcgcct attcgcccga ccgcgtattg
     1561 gatcctttcc tgtatctcgc ttatcaggtg ccttatttcc tttgcggcgg cattgctcct
     1621 ctccgaaagt ttgcgtatct cttccgccag cacggtaaat cctttgccct gctgtcctgc
     1681 ccttgccgcc tcgattgcgg cattcaacgc cagcaggttg gtctttgagg ctatttccgt
     1741 tatggtatca gttatattac cgatctttcc cgaggaatct tcgagttcat ttattgaagc
     1801 tgctatctgc ttcacggcgt cggtcacgtt gcggatcact tcttccgctt cctgtacgga
     1861 tctctccccg ttcgcggcta tctcgatggc tttaatgccg tcctccacgg ctttccctgc
     1921 ggtcgcggcg attatatcgg tgtcgtctgc cgccgctgct gtttcggtcc cggaagtttt
     1981 tatcacggcc atcaggccat ccattatgct cctgaaacct ttcgatacct tgccggtgtt
     2041 ctccctgctg tgcgagaaca actcaacgag gctgttcctg agctttccga tgctggtcga
     2101 ggtagtcctg acctgttcga gcatcttttg ctgtccgtcc aggactacat tgaccttttc
     2161 acccaactcg cccagctcgt cgcgtctcct gtccctgacc ctgcccgaaa ggtcgccctt
     2221 gctggctttg tccagcaggt tcgtcatgtt ccgtatcggc ccgagtatat tgcccgaaag
     2281 ccataaagcc gcaaacatgc cgataaacag tgatatgaag gaaataaccg caagcgtaat
     2341 cagcaggttg tttttaagtt cctgtgagcg ttccaggtcg tctgcaaggt aagacttgta
     2401 tgaagtttca agtttgctaa gcaattctat ctgtcggtca tacaaatcgg atgcaccggc
     2461 aaaactctcg gaaaggccgt tatcctcaag acccttttta gcctccagca gcgatccgat
     2521 cgcctcttcc atccggctgc actgtgccgc tgcttccgca gcaagtgcgg catgctcgga
     2581 attaagttca gatcccagtt tttccagttc agcagctgcg ctttttattg cattcaaggc
     2641 cgattccgtt tccggactgc cggcaaccgc cagcccggcc tgtgccctgc catatgcttc
     2701 tgccgtcagc gtcagcgccg cattgacgga tgacagcctc tgcaatgctt cctggaggcc
     2761 tgcagccgac aagccgctga caatgttcct gatatccgtt ccgctggcca gcacggcatc
     2821 caggtatgtc cttacactat cagcaagcac ctggtcgtaa accgcaggag atatgccgga
     2881 aggagaagat gacccaggct gcgttcccgt cggtccggct gcaggcagtt ccactgagac
     2941 cggtgcagca gacgtatcag ccggctgtgt tccgcccgca ctgtacgcag ttcctgcgga
     3001 actccggcca gtgtaaccgg caggctgctg tgccttcagc acctccagct ccgtcttcag
     3061 cctgcttatc tcctctagca aagcatcaat gttcgtctca agttcgttta tccttatctg
     3121 ctgcttatct ttcagaagca gcagactccc gttggcttcc gccgctgact tgtactcctg
     3181 cagtaccttt tcggctgccg gcagcagttc atccagcgct gcaatctgct ccgcagacag
     3241 agaaccaata cctgccgcat ccttcgaaag ttttgcagcg gcagcatcga tctgtttaag
     3301 tattacgctt ttcagttctg tttccttttg aatcagcgaa tcatactgta aggagaaatc
     3361 agccagaaga gcgttatatt ctgttggatc cgacttttcg atccctttgg agatatctgt
     3421 tttgcacaac tgggcaaact ggtccgccag gttcttcaga ttttcaagct ctgcccgttc
     3481 gctgtcctcg gactgtgaag ccagcctgtc gacagcttcc ctgattttgc tgttttcagc
     3541 ctcgacagcg tctgccggca aggcatccct gccgaccaca gcactggaca aagcctggta
     3601 ctgtttgtgt accataccgc tgatctcacg gacgcttatg atgcgactgt tgttgctgtc
     3661 agcacttgcg gcgatctccg acaccatcat gttgtaaccg ccgagtgtaa ctgcaagcgt
     3721 tgctgctgag catatcaata tcgtcgctgc aaaagcaagg atcagtttcg ccctgatttt
     3781 tatcatcatt ccatctccgc taccctgaag atcagccgcc aatagttcaa atgcgggaca
     3841 ttcctcaact ataaaggaat accctccact gaggagtgtc cccatacctc tttctcacca
     3901 ttacgcttac cattatttat tcgataaagt ctgacaaatt cctccttaat tgtgtcccga
     3961 cttgcgttcc ggcaaaaaat ttgacattgt tgccgatttc agatattaat aattgttgtg
     4021 atatattatc cgaaaaaaag aggattattt attgcggtgt agaatttata tattgccttt
     4081 ttttcggaca tcactgtaca aaagacaaga atacggctgt ttgggcattg ttgcaggatg
     4141 gttcgatatg aaaaagggaa aggctaaaag agcgcagaaa aaatatcttt caatagttct
     4201 tgtccctcat tcgtcaagtc gtgtaaaagt attgagattt aatttatttt acgccaagtt
     4261 ggtcacattc ttcgtggtac ttatcgctgt tttcgtgttc ggcggcttgt acatatctaa
     4321 actgcttgat gaaaataata tgctcaagca aaatgtcagc aacctttaca gcacgactgc
     4381 ggagcagagc acgctccttc agaaaaaatc ggaagagatc gaatcgctga aaagagaaag
     4441 cgaagcgttc agggaagtcg taaacgacag gatagaagaa ttcaccgaga agttcaacca
     4501 actgaccgat gaatacctgg aagagcagag cgccagggta agccgttccg gcgaaaggac
     4561 tgaaacggct ttcgcctccg acatgcggga actgaaaact tccctggaca aactgatcca
     4621 gttatacagc agatccaaac ggccggacgc tgatattgaa gccgtagaag aaaagataaa
     4681 cgcatttatg gaagtgatcc ctacattatg gccgaccgaa ggcaggatca ccgatcagtt
     4741 cggctacagg aaggatcctt tcacacgcag gaaatcatat cacagcggac ttgatatagc
     4801 cgccgatgta ggtacatcca taaaggctgc cgcaagcgga aaggttatct atgccgatta
     4861 cacatacgcc acgggacgca cggtcaagat cgatcacggc aatggcttcg tcacagtata
     4921 cggccactgt tcaaaaatcc tcgcagaacc cggccagcaa gtgaaaaaag gcgaagttat
     4981 agcaaaagtg ggaagtacag gccgcagtac aggtcctcac tgccactttg agatccattt
     5041 gttcggaaca gccatcgatc ctctggagta tctggagaag aaataaagac agcggaaaac
     5101 ggcattgcca gctccggaat cggcaatgcc gtcaggaggt cgatcaaaga aaatcagggg
     5161 aggtaatcca tgttgaaaag gaaagcaagt ttccgtgaag gagtagacac gcttgtcggc
     5221 agcaactcgg tattcaaggg gaatatcgag tccgaaggca ctgtgagggt ggacggaaaa
     5281 gtgatcggcg atatcaaggt cgaaggtgat gtgtatatcg gcaacggcgc tgcagttgag
     5341 ggaaatgtgg atgccggcaa cgtatatctt gcaggaatga taaaaggaaa catcgtggcc
     5401 aagggacttc tcaagatact ttccacagcc aaactgtatg gcgacatcaa aatcaacagc
     5461 ttcgttgctg acgagggcgc tttgttccag ggtaaatgcg agatgatcga cgggacagaa
     5521 tccgagaaac cacccgaaaa acacagaagg aactataaaa agagcaccgt gcttgaggat
     5581 atctacgaag acagccacag cgccgggacc gacgcatgac ggcagcccgg atcatgatgc
     5641 gtcctaaagc gcaataaagc cgtctatgta cagttctgcg aacagcagaa cggcgcccat
     5701 gataagcaat gcgatcaaga ggcggagcaa caggcggatc ctgttgatgt tccgcctttt
     5761 tatataccac catctgcggt agaggttact gttcctgatc acgccggcac ctccgctgca
     5821 ccttttcccc aactgtccgg atgcagcact gttttacgat cataatatgc cgtttgcatc
     5881 attttggtta caggtaccgc agtccatatt tttgagtatc tctaccgaca tacagcgttt
     5941 gaaagtgatt tcagtccgga cgcagagatc aagtttattt tataaaagac agaataaaat
     6001 aagtatggcg caggcgatcg gcgatcggaa ccgaatttta cacccaatct cctgatgtgt
     6061 tataatgtag tttggcattt ttgctgttgg aactgtcagt acggcaaaac agtctaatca
     6121 acctgaatag cacgtattag ctgtttgttc tgataggagg ataagatgaa attcgagaag
     6181 aaaccttccg caggcggagc ggaaaaaaaa cataataaac cgaaaaaagc tgacaaaccg
     6241 aatagaaaga aaaattcttc attcataaaa aagttcgcat tgactttgat cgcctgcatc
     6301 cttctgttca tttttgcgat agggtgcgcg ctgaccggcc tggtaggcgg ggccgtctat
     6361 gcatatatcg agacggcaga acccatatcc gacgaacagc tgatcgccaa ggcgtcaagc
     6421 aggaccaccc tgatctatga cgcaaaaggc aacatcatac ataaactcac aggcaaagac
     6481 aatatggaca gcgaacctgt caccgacaaa gaggtgcccc agtacctcaa agacgccatc
     6541 atttctatcg aggacgagcg tttcttccag cacccgggcg tcgatatcca gggtatcatt
     6601 aacgcgggcc tgggattcgt caagagtatc atcaccggca acgatgaggg gctcaggggc
     6661 ggcagcacca taacgcagca ggtggtaaag aacataacgg gcaatacgac gcgttcaatc
     6721 aagaggaaag tgcaggaagc atacgcggcg atactccttg agcggaaact cgagaagtgg
     6781 cagatactcg aactttatat gaacctcggg tactggggca acagttgtac cggcgtccag
     6841 tcggcttcca agaaatactt cgggaagcct gtaagcgagt tgtcactggc ccagtgcgcg
     6901 ctgcttgccg gtataaccaa aaacccggcc aaatataatc cttttaccga aaaaggccgc
     6961 gagtacgcca aggaacgcca ggaaataata ctcgggaaga tgctcgaact ggggaaaata
     7021 tcccaggagg agtatgacca ggctatcaaa gaagacctgc ggtacgccct gaatactggt
     7081 tcacagaaaa ccgcgaatgt ccagagctat ttcgtcgacc aggtcatagt ggatgtccgc
     7141 aacgcgctga tggaggaaat gaacatatcg tcgaccatgg ctaattacat gatatatggc
     7201 ggcggactcg aaatatatac aacgatggat cccaagatcc aggcggatat ggactcggtg
     7261 ttcatggacg acacatactt cccgctggt
//
LOCUS       DAOSCC010000121         7284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6559_length_7284_cov_4.653064, whole genome shotgun sequence.
ACCESSION   DAOSCC010000121 DAOSCC010000000
VERSION     DAOSCC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_7284_cov_4.653064"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLG72_07375"
     CDS             complement(<1..588)
                     /locus_tag="PLG72_07375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOJ80654.1"
                     /translation="MGIEKSLFGKMPDGTEIYLYRLSNRNNMSVGIINYGGIIVSIDV
                     PDRDGHIDDVNLGYDNLEQYLKLGQYFGAIIGRHGNRIENAEFELNGVTYHLAKNDGR
                     NHLHGGYRGFDKVVWQAEPIDSEDAPALRLSYLSPDGEENYPGNLDVKVTYTLTGDNS
                     LRIDYFAVSDKDTVVNLTNHAYFNLSGHGSGDILDH"
     gene            complement(1003..2007)
                     /locus_tag="PLG72_07380"
     CDS             complement(1003..2007)
                     /locus_tag="PLG72_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ80655.1"
                     /translation="MKIIRVLLAAILVISLILSFTACGEKDLVRIRLNEVTHSIFYAP
                     QYVALNLGFFEEEGLSIELINGQGADKVMTAVLSNQADIGFSGPEAAIYVYNEGKEDH
                     AVVFAQLTKRDGSFLVGRKPEPDFKWTNVKGKLIIAGRKGGVPEMTLEYVLKKHGVMP
                     GVDVELDTSTQFALMAGAFTGGMGDYVALFEPVASTLEREGRGYIVASIGRDSGEIPY
                     TAYYAKKSYIEKNPETIQKFTNAIYRGQVWVDTHTPEEVAEALKDSFPDSDIDLLVTV
                     VKRYKEQDTWCTDPIMKKESLDLLQEVMKTAGELEKEAPYEELVDNSFAEKAIKNIKK
                     "
     gene            complement(2062..2880)
                     /locus_tag="PLG72_07385"
     CDS             complement(2062..2880)
                     /locus_tag="PLG72_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80656.1"
                     /translation="MNSNGISKEHMEYLKKVRMRKTLILVLRLFLLVAFFALWELLAR
                     LKLIDPFITSQPSRIADTVAKLYHEGKLFYHTGITCLETVIGFMLGTTAGTLIAVILW
                     WSELLSRVLEPYLVVLNSLPKIALGPIFIVWIGAGPSSIIAIALTISLVVTILEVLNG
                     FVSVDGEKIKLVRTFGANRLQVLTKVVLPSSYPVIINALKVNVGLSWVGVIVGEFLVS
                     KAGLGYLIVYGGQVFQLDLVMTSVLILSAAAALMYQGVVYIEKSLVRSGKHTSL"
     gene            complement(2926..3693)
                     /locus_tag="PLG72_07390"
     CDS             complement(2926..3693)
                     /locus_tag="PLG72_07390"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80657.1"
                     /translation="MGAIVEVHNLGMKYHSLNGEIAALRDINLEVEEGEFISIVGPSG
                     CGKSTLLSLLAGLLKPTEGSVVIGGRPVTGPDNQVGYMLQKDYLFEWRTIYENVILGL
                     EITRTLNSRTKEYVLKLLETYGLSEFKDKYPNQLSGGMRQRAALIRTLAVNPQILLLD
                     EAFSALDYQTRLAVAEDIYSIIKRENKTAILVTHDIAESISMADRVVVLTKRPGTIKK
                     IYHVRLTCDRKTPMSRREAPEFRHYFNNIWKDLDIRV"
     gene            complement(4031..5161)
                     /locus_tag="PLG72_07395"
     CDS             complement(4031..5161)
                     /locus_tag="PLG72_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80658.1"
                     /translation="MVLDDLKSRNIPDVLRMNDGRYADSPELWRVRRKEILELLGREI
                     YGFSPPPPQEVHADILGSYDEAFGGKAAHYTIRLSFDTPGGEFSFPFNLAVPKNVARA
                     PVFLLISFSPNIPDMLFPVEEILDEGFAAASFCYADVVPDTDDGFSAGIASMYGNGRR
                     EPHSWGKISMWAWAASRVMDHLQSRNDIDTGKVIPVGHSRLGKTVLWCAAQDERFAGC
                     VSNDSGCSGAAITRGKKGERVSNITGAFGYWFCDNYRKYADNEDNMPFDQHYLLSLIA
                     PRPLYVASASRDEWADPESEYLGCIAAGRVYEMLGAKGVGYGSEYAQAAANGPVNGSD
                     YPKPGDVLHGGNVAYHLRSGTHFLSRYDWNCFIRYFRKQFGL"
     gene            5382..6458
                     /locus_tag="PLG72_07400"
     CDS             5382..6458
                     /locus_tag="PLG72_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOJ80659.1"
                     /translation="MYFRTLEDFRNYIEGSDFKSSEQLMIMAGDRSSGQIPEMIELLN
                     KRNISFFGAIFPEIIIGSKTRREGFVVEKLRPIYSSLVFPFMMKFSQDRNKLQGATAI
                     VFVDGLSGRMKDLTDTICEKLGSTVTYVGGGAGFKDMKHRPCIFNSTGIYKDALYVCI
                     VKNSTALAVEHGWKILRGPFYVNRSYDNVLSGLDNCSAFDIYRHVIEEEENLTLFRED
                     FFIYAKDHPFGILDEDGKVIVRDPVAVNDDDEIICVAGIPEGSDLYILHGDVDTLLES
                     SLQITRVQGSISARPKRYLPLVFDCLSRAMFLGKLISEELDNIQANLSYGVEGALSIG
                     EIACRKNGELVIHNKSTVLALMEL"
     gene            6520..7086
                     /gene="thpR"
                     /locus_tag="PLG72_07405"
     CDS             6520..7086
                     /gene="thpR"
                     /locus_tag="PLG72_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ80660.1"
                     /translation="MICIRAFIGIDFSDECRKYIFDLQQRLKKYAVRGRWKHIGNFHL
                     TLKFLDEISAEQKKQIDMSMKQLCGAFKPFSLEISETGIFKGNDAIRVLWLGIGGDLG
                     TLHRLAGSIDSSVSALGFPPEKRRYKPHITIGQDIVFECPFENIADSIGRISYGPIKV
                     EKICLFKSEQVQNKRVYTIVSEYGLKGN"
BASE COUNT         1812 a         1975 c         1712 g         1785 t
ORIGIN      
        1 gtggtccagg atgtccccgg atccatggcc ggacaggttg aaatatgcgt ggttagtgag
       61 gttcaccaca gtatccttgt ccgagaccgc aaaataatcg atccttaagc tgttgtcccc
      121 tgtaagagtg taagtgacct ttacatcgag gttcccggga taattctctt caccgtccgg
      181 gctgagatac gaaagccgga gagccggggc atcttcgctg tcgatcggct cagcctgcca
      241 caccaccttg tcgaacccgc ggtacccgcc gtgcagatgg ttcctgccgt cgtttttcgc
      301 cagatggtat gtcacgccgt tcagttcgaa ttccgcattt tcgatcctgt tcccgtgcct
      361 gccgatgatc gccccgaaat actggcccag tttcaggtac tgctccaggt tgtcatagcc
      421 gaggttcacg tcgtctatat gcccgtccct gtcaggcaca tcgatgctta cgatgatccc
      481 tccgtagttg atgatgccga cggacatgtt attcctgttg ctcagcctgt acagatatat
      541 ctccgtaccg tccggcatct tcccaaaaag gcttttttca atacccatag aaatacctcc
      601 gttcagaaat gtgtgccgca ctgtgcaagt attctgaaaa acacccgcaa caagtatttt
      661 accatatatt attgtctata ccaaacttta tcgtactccg ttcatgtgca atttaacagg
      721 ttttgtccat tgctatgaaa tctatcatat accataatgc tgtattgaag acataatgtc
      781 tcctgttatt ggagccattc aataaaaact gcagaaaatt cggacaaaat tgaaaatttg
      841 aacacagcgg ccggcaaact gaccaacgcc gctgcggcat aaaagttgct gacgctatac
      901 aacaaagaaa aaacgctgac atcgatttat gccagcagaa tatacgcgaa ctatgggcaa
      961 catcatcctg cacaggactc ggtaaacacg ggttcttact gctcacttct taatgttctt
     1021 tatcgctttt tcggcaaagc tgttgtcgac cagttcctcg tacggagcct ccttctcaag
     1081 ctcgccggca gtcttcatta cttcctgcag cagatccagc gactctttct tcattatcgg
     1141 gtcagtacac caggtgtcct gttccttgta acgctttaca acggtgacga gaaggtcaat
     1201 atcggaatct gggaatgaat ccttgagggc ttcagccacc tcttccggag tatgggtatc
     1261 cacccatacc tgtcccctgt aaatggcatt ggtaaatttc tgtattgtct caggattctt
     1321 ctctatatag cttttcttcg cataataggc ggtatacggg atctcgccgc tgtccctgcc
     1381 tatggaagca actatgtatc ccctgccttc cctttccagg gtcgaggcca ccggctcaaa
     1441 cagcgcgaca tagtccccca tacccccggt gaatgcgccg gccatcagcg cgaactgggt
     1501 gctggtatcg agttccacat ccacgccggg catcacgcca tgcttcttga gcacgtactc
     1561 cagcgtcatt tcaggcacgc cgccttttct gccggcaatg atcagcttgc ctttcacatt
     1621 ggtccatttg aaatccggct caggtttcct tcccacaagg aacgatccat ccctcttcgt
     1681 cagctgcgcg aatacgaccg catgatcctc cttcccttcg ttatacacat agatcgcggc
     1741 ctccgggcct gaaaacccga tgtctgcctg gtttgaaagc accgctgtca taaccttgtc
     1801 cgcgccctgc ccgttgatca gttcgatgga aaggccttct tcctcgaaga aaccaagatt
     1861 gagagcgaca tactggggtg cgtagaaaat cgagtgcgtg acttcattga gccttatcct
     1921 gacaaggtct ttctcaccgc atgctgtaaa gctcagtata agacttatca caagtatggc
     1981 ggctaataaa actcgtatga ttttcattga tctccctcct tccgtccaga atcttcctgc
     2041 atcctgttta tatgcaaatc atcacaggga cgtgtgtttc ccgcttctga ccagtgattt
     2101 ctcgatatac acgacgccct ggtacataag ggcggccgct gccgaaagga tcagcacgct
     2161 ggtcatgaca aggtccagtt ggaagacctg ccctccgtac acgatcaggt acccgagccc
     2221 ggctttcgaa acgaggaact ctcctacgat gacaccgacc cacgaaagcc ctacattaac
     2281 tttaagcgca ttgattatga ccggatacga cgacggcagc accactttcg tcagcacctg
     2341 cagcctgttc gcaccgaaag tcctgaccag ctttatcttc tctccgtcca ccgacacgaa
     2401 gccattgagc acttccagta tcgtcacgac aagagatatg gtaagcgcta ttgctatgat
     2461 ggaggacggc cccgcaccga tccatacgat aaagatcgga cccagcgcga ttttgggaag
     2521 actgttgagg acgacaagat acggttcaag caccctggat aaaagctcgg accaccagag
     2581 tatcacggct atcagcgtgc ccgccgtcgt tccgagcatg aacccgatca ctgtctcaag
     2641 gcatgttatc ccggtatggt agaacagttt gccctcatgg tacagtttgg cgacggtatc
     2701 cgcgatccgt gacggctggc tcgtgatgaa cggatcgatc agtttcaggc gcgccagcag
     2761 ttcccacagg gcaaagaagg caaccagcag gaacaggcgc agtacaagga taagtgtctt
     2821 cctcatcctg acctttttca gatattccat atgttctttt gaaatgccgt tgctgttcat
     2881 accgatcaac tccctggtac gacatggatg ccggaaaccg gaccgctaaa cgcggatgtc
     2941 caggtccttc catatgttgt tgaagtaatg cctgaattca ggagcctccc tgcggctcat
     3001 gggtgttttt ctgtcgcacg tcagccggac atggtaaatc ttttttatcg tgcccggtct
     3061 tttagtgagc accaccacgc ggtccgccat gctgatgctc tctgcgatat catgggtgac
     3121 aagtatcgca gtcttgtttt cccgttttat gatagagtag atatcttcag ccactgccag
     3181 cctggtctgg taatcaaggg cggaaaatgc ctcgtcgagc agcagtatct gcgggtttac
     3241 ggccaatgtc cttataagcg ccgccctctg ccgcatacct ccggagagct ggttcgggta
     3301 tttatccttg aattcggaaa ggccataagt ctcaagaagc ttcagcacat actcctttgt
     3361 acggctgttc agcgtacggg tgatctcaag tcccagtatg acgttctcat aaatggtccg
     3421 ccattcaaag agatagtcct tctggagcat atagcccacc tggttgtcag gtccggtcac
     3481 aggccggcct ccaatgacga ctgatccctc cgtcggcttc agaagtccgg caagaagaga
     3541 caggagcgtc gatttgccgc acccgctcgg gccgactatg ctgatgaatt cgccttcttc
     3601 gacttccagg tttatgtccc ttagcgccgc gatctcacca ttcagcgagt gatatttcat
     3661 tccgagatta tgtacttcca ctatcgctcc caaagtacca tcctccgaat cgagtgctgc
     3721 aggctgccat gcccgtatct gccgcatcgt atccggctat ccgaagggca ggaattttat
     3781 gtacattaca gtatataaat cttttgctga aacagtgaat gaaaattggc ccgccatcaa
     3841 cgggaaggct cattcgacag ccggagacgc atcatgcgac gcaggctcac agacaggaac
     3901 gccggccgga aaaaagcata aaggagaaag acaagccggt agaagacagc agcaatagtc
     3961 gcaaatgact gaaaaaggct gccttaaccg gcagcccttc actcatggta tggatcatca
     4021 acaatgctga tcataagccg aactgcttcc tgaaataccg tatgaagcaa ttccagtcat
     4081 atctgctcag gaaatgggtg ccgctgcgca ggtggtacgc tacgttccct ccatgcagta
     4141 catctcccgg cttcggataa tcggatccgt tcactgggcc attggctgca gcctgcgcat
     4201 attcgcttcc gtaaccgaca cctttcgctc ccagcatctc atacaccctg cctgcagcaa
     4261 tacagcccag atattccgac tcgggatctg cccactcgtc ccgcgaagcg ctggcaacat
     4321 acagcggtct cggagcgatc agcgaaagca ggtaatgctg gtcgaaaggc atattatctt
     4381 cattatcggc atatttgcgg taattatcac agaaccagta cccgaaagcc cctgttatat
     4441 tgcttacgcg ctcgcctttt ttaccgcggg ttatcgctgc gccggaacat cccgaatcat
     4501 ttgatacgca tcctgcaaac ctttcatcct gcgcggcaca ccacaaaacc gtttttccaa
     4561 gcctcgaatg tcccaccggg attactttgc ccgtatcaat atcattcctg gactgcagat
     4621 gatccatcac cctcgacgca gcccaggccc acatggaaat cttcccccaa ctgtggggct
     4681 cgcgacgccc gtttccgtac atcgaagcaa tgcctgccga gaacccgtcg tcagtatccg
     4741 gcacaacatc ggcataacag aacgatgccg ctgcaaagcc ttcgtccagg atctcttcga
     4801 ccgggaagag catatccggt atgttcgggc tgaatgaaat gagcaggaaa acaggtgcac
     4861 gggccacatt tttaggaacg gccagattga acgggaatga aaactcaccg cccggcgtgt
     4921 caaaactcag cctgatcgtg taatgcgcgg ctttccctcc aaaagcttca tcatagcttc
     4981 ccaggatatc agcatgaact tcctgcggag gaggcggtga aaatccatat atctcacggc
     5041 caagcagctc gaggatctct ttgcggcgta cccgccaaag ttccgggctg tccgcatacc
     5101 gtccgtcatt catgcgtaaa acatcgggga tattgcggct tttcagatca tccaggacca
     5161 tttggttccc tccatccgga tatgtcattc tgttataatt ataacgcata aatgaaagaa
     5221 aaggcactca aaagcctcgt tgaaacattt aaatccggat tttcacggga ttatccattc
     5281 cctggccaat atttcagcga tttgcaatat attgcaggtt tttttgatag tgtggtaaaa
     5341 tgtactaaat ggcgaacagt gatgatatcc aggaggcata tatgtatttc aggaccctgg
     5401 aagatttcag gaattatata gaaggttcag attttaaatc ttccgagcag cttatgatca
     5461 tggccggaga cagatcgtcc ggccagatac ctgaaatgat tgaattgctc aacaagcgga
     5521 acatcagctt ttttggcgcc atcttcccgg aaatcatcat tggcagcaag accagaaggg
     5581 aagggtttgt agtcgaaaaa ttaaggccga tatattcctc cctcgttttc cccttcatga
     5641 tgaagttttc acaggacagg aacaaactgc agggcgctac ggccatcgtt tttgtggacg
     5701 gcctgtccgg caggatgaaa gatctgaccg acaccatctg cgaaaagttg ggaagtaccg
     5761 tcacttatgt cggcggcggc gccggattca aggatatgaa acacaggccg tgcatattca
     5821 acagcacggg catctataag gatgcgctct atgtatgcat cgtcaaaaac agcaccgccc
     5881 tcgccgttga gcacggatgg aaaatactga gaggcccgtt ctatgtcaac aggtcatacg
     5941 acaacgtgct gtccggcctg gacaactgca gcgcattcga catttaccgg catgtgatcg
     6001 aagaagagga gaaccttaca cttttcaggg aggacttctt catatatgcc aaggaccatc
     6061 cgttcggtat cctggatgaa gacggcaagg tcatcgtaag ggatccggtg gctgtcaatg
     6121 acgacgacga gatcatatgc gtagccggga tccccgaagg ctcggacctg tatatcctgc
     6181 acggcgatgt agacacactg ctcgaatcct cgctgcagat aaccagggta caggggagca
     6241 ttagcgcccg gcctaaacgg tatctgccgc tggtgtttga ctgcctgtca agggcaatgt
     6301 tcctgggaaa gctcatctcc gaagagcttg acaacatcca ggcgaatctc agctatggcg
     6361 tcgaaggcgc cctttccatc ggagagatcg catgcaggaa aaacggagaa ctggtcattc
     6421 acaacaagtc caccgttctt gccctgatgg aactgtaaca ggatacaatt tggaaaatgt
     6481 gataaaataa gctttgattc ccgaatacat gcgtaaagga tgatctgtat cagagctttc
     6541 atcggtatag actttagtga cgaatgcagg aaatacatat tcgatctgca gcaaaggctc
     6601 aagaaatatg ccgtcagggg cagatggaag cacatcggca actttcacct taccctcaag
     6661 ttccttgacg agatcagcgc ggaacagaaa aagcagatag acatgtccat gaagcagctt
     6721 tgcggcgctt ttaagccttt cagccttgaa atctcagaaa ccgggatatt caaaggcaat
     6781 gatgccatac gcgtcctttg gctgggtatc gggggcgacc tcgggacgct gcaccgactg
     6841 gccggcagta tcgacagttc ggtgtccgca cttggtttcc cgcctgaaaa aaggcgctac
     6901 aaaccgcaca ttaccatcgg ccaggatata gtattcgagt gtccgtttga aaatatagcg
     6961 gactcgatcg gcaggatatc atacggcccg attaaagtgg aaaaaatatg cctgttcaaa
     7021 agtgaacagg tgcagaacaa gcgtgtctat acgatagtat cggaatacgg cctgaaaggc
     7081 aattagatat aaaaaaaccg gctttgtatc caaaagccgg cacttaattt tgctgatccg
     7141 cctcattatc agcccccctg ggatctcacc ctttgacatc cctgccttct gctgctgtct
     7201 gcggctccat gttcacatcc actaagccga agtattcctc aagagtaagg cctttttcca
     7261 ttatgtcggt cgccgcatcc ttgc
//
LOCUS       DAOSCC010000122         7267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6583_length_7267_cov_4.268719, whole genome shotgun sequence.
ACCESSION   DAOSCC010000122 DAOSCC010000000
VERSION     DAOSCC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6583_length_7267_cov_4.268719"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..2384
                     /locus_tag="PLG72_07410"
     CDS             249..2384
                     /locus_tag="PLG72_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80661.1"
                     /translation="MKTKLISLMLVLVLMVCIATVPSMADTSENSTFAYLMYADASWT
                     NQYWGTDDSSVKAAKADITGPGEYTVGLDFTGTEAGVASGIAFTALGIQNGELAFPYY
                     TIELTSIKINGEDIEFTKGYTSSDDGITTRMNIYNEWVSELPYDARSFDNNISDASAV
                     IVSKDAFASVRTIEVSFKLHAPQDVAYLMYADSTWTYQYWGDPVDTGVVAKDATITGS
                     GTYTVSLDFTQTADKKASGLAFTALGIKTGERSFPGYFIKIKDIKVNGKSISLKKGYT
                     SSDDKIVTRMNIYNEWVANLPDDARSYDGKVDDANWIIVDKDAFASVETVEVTFDFVQ
                     PSAEAYIMYADTAWKSTYFNDGTETAIIPTNAKVTDPGHYKVALDFRSTEDKAASGLA
                     FTAVGIKDAEISHPGWFIKIESIKVNGREIEFTKGYTSSDNGKETRMNIYNEWETALP
                     DNARSYDKKLDDASWKIVDPAAFANVETLEIEFAFIRGERPKKEKDADIDIDAALAAD
                     YNAYFGIQTESYIFRNAWNEKNYGKETDYFTHLTGWDSDNNAVDYGGTLKDAAVTGNG
                     TYTVGVDLGPMGLGSDQTIRMLYVSTDIPSVLVDKGYVTISDVKTSIDGGKAQTAFTV
                     NTDGDYVQIDILNEYTSTGTEAISYKMPTKSVTITFTVSGLSKDAGSAGASASGLPKA
                     GGVPGELMYVLGCAVTSLGIYMKARKKKV"
     gene            3079..3978
                     /locus_tag="PLG72_07415"
     CDS             3079..3978
                     /locus_tag="PLG72_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ80662.1"
                     /translation="MEMPEKYTLFLMLLPIIILAFVFLYLPLYGWRYSFFDYEAGGTL
                     SRENFVGFKWFTILFRNQATVNDILRVLRNTLAMSGLGILTSWVPMAFAIFLNEIRSN
                     RFKRVVQTVTTIPNFISWVLVYAIALAIFSTEGFLNSFLELFNANRNINWLMSSEHTW
                     LKMLLWGIWKSLGWSAIVYIAAIAGIDQELYEAAKIDGAGRFQRIWHITVPGLMPTYL
                     VLLLLTIAGALSNGLEQYLMFQNANNKDVIEVLDLYVYNIGITGGRIPLSTVIGMTKS
                     LVSIALLMFANRVSKSVRGEGII"
     gene            3989..4927
                     /locus_tag="PLG72_07420"
     CDS             3989..4927
                     /locus_tag="PLG72_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80663.1"
                     /translation="MNNSTELKLKSARRSGFRRSPGEVAFDVINVTVFTIFMLLCIFP
                     FYYLFINTISDNDLVLKGAITYYPVGINISNYIALKEVNDLGSAFIVTVSRTIIGTAL
                     MVAASALAGYLVTKKKLWKRSFWYRYLVITMYFNAGLIPWYMNMVMLGLTENYMAYII
                     PGIVAPYNIILVKTYVESIPGELEESAFIDGASTFTIFRKIIWPLSKPILATIAVFGA
                     VGNWNSFQDSLLLMKSSPKLFTLQHRLYFYLVKASDLESIMAGGTGTNVVLENTLNSK
                     VVKYTIAMATIIPILLVYPIMQKYFVKGIMLGAIKG"
     gene            5000..6991
                     /locus_tag="PLG72_07425"
     CDS             5000..6991
                     /locus_tag="PLG72_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80664.1"
                     /translation="MKKLKGFKKIVSTILSVMLILSMLFMITGCGQDGGTADGDAAVQ
                     GTDGSASDASGGQASAGGDASADGTSGNAGGTTADGQPAAGDANDESGTGLTEDQPMV
                     QEAAGSEEIEVVESTDKAEKPSELITLDVYSQLANWSGNQIGWYAALLEDKFNVRLNI
                     IPDAEGTYETLIESGDMGDIVVWGDNGAEYKKAVQLGLLYDWEEGDLCKTYAPMIWNN
                     CKIALETNRKISGDGHIYGIGHSVALSKDDHQSFFYTWDLRWDLYKKLGYPKVKNLDD
                     YFNVLKQMKEICPKDENGRETYAVSLWPDWDGSMVMYVKAMATAYYGYDELAVGLYNP
                     TNGEYYDALMENGPYLEMLKFFNRLYQNGLLDPNSMTQTFDQMSDKVKAGGTFFSIFN
                     FAGSASYNTTEHMKQGKMMLPMVPEDASPAVYGMSMLGGNRVWSIGAYTEYPELCMEI
                     LNYLFTPEGAMTIWYGLPELMWYYDDNGYTHFTDLGLKCNRDPHYDLGGVTWTSPETG
                     KTYKLGGNFTDGMLQINNTTWVIDTKNPDSNGETFNKDTWRSMAGPAQSTIEQDWRDY
                     YKVTTVNEYMKKGKYMVVPGTSYSEPKRSDELELIWTQVTQAIKQYSWRAIYAKNDGE
                     FNYHVQQMIKVCKDYGYDQVREWSRQQAATRYKLQKAEN"
BASE COUNT         1974 a         1740 c         1901 g         1652 t
ORIGIN      
        1 tgcctgccgg gcaatataat gcggatcgaa ggacacagca aataattatc tgaacgggac
       61 cgcgtcggcg gttgagatag agaataagac ggggcttccc tcgttggcag ggaggacgac
      121 attcgtactg taaggcagta tctgcaggat gtgttttact gcagaaggaa gtgccgtttt
      181 actttccgaa gccgcggacg ggtatcctgt catactacca ccaaatatta agtgaaagga
      241 gtaatataat gaagacgaag ctaatttcac taatgctggt attggttctg atggtctgca
      301 tcgcgacggt accttccatg gcagatacga gcgaaaacag cactttcgcg tacctgatgt
      361 atgctgatgc cagttggacg aaccagtact ggggcacaga cgacagcagt gtcaaggcgg
      421 ctaaagcaga catcaccgga ccgggtgaat acacggtcgg ccttgatttt acaggaacgg
      481 aagcgggcgt ggcaagcggt atagcgttta cagcactcgg catccagaat ggtgaacttg
      541 cgttccccta ctatacgatc gaacttacgt caattaagat caacggagaa gatatcgaat
      601 tcacaaaagg ctatacaagc tcggacgacg gaataacgac ccgcatgaat atttacaacg
      661 aatgggtatc ggaacttcca tacgacgcaa ggagctttga caacaacatt tccgatgctt
      721 cggctgttat cgtatccaag gatgcgtttg cttcagtcag gaccatcgag gtatccttta
      781 aactgcatgc gcctcaggat gtcgcatacc ttatgtatgc cgattcgacc tggacgtacc
      841 agtactgggg cgacccggta gatacaggcg tagttgcgaa agatgcgacg atcaccggat
      901 ccggtacata taccgtaagt ctcgatttca cgcagacagc ggacaaaaag gcatccggcc
      961 ttgctttcac cgcactcggg ataaaaaccg gtgaaagatc tttcccgggc tacttcataa
     1021 agatcaaaga catcaaggtc aatggcaagt caataagcct caaaaaggga tatacaagtt
     1081 ctgacgataa aattgttacc cgtatgaaca tatataacga atgggtagcc aatcttcccg
     1141 atgatgcaag gtcctacgac ggcaaagttg acgatgccaa ctggataatc gtcgacaaag
     1201 atgcattcgc ttccgttgaa acggtcgagg taacttttga ttttgtacag ccgtctgctg
     1261 aagcatacat catgtatgcc gatacagcat ggaaaagtac atacttcaat gatggcactg
     1321 aaactgcaat cattccgacc aatgcaaaag tgacagaccc cggacattac aaggtagctc
     1381 ttgatttcag aagcacggaa gacaaagcgg catccggcct tgcatttact gcagtcggaa
     1441 taaaggacgc tgaaatctcg catcccggct ggttcataaa gatcgagtcg atcaaggtca
     1501 acggcaggga gatcgagttc acaaaaggct atacaagttc ggacaacggc aaagaaaccc
     1561 gcatgaacat ttataacgaa tgggaaaccg cgcttccgga caatgcacgt tcctacgaca
     1621 agaaactgga cgatgcaagc tggaaaatag tcgacccggc agcctttgcc aatgtggaga
     1681 cccttgagat cgagttcgca ttcatccgcg gtgaaagacc gaagaaggaa aaagatgcgg
     1741 atatcgacat agatgccgca ctggcagcgg attataacgc ttacttcggc atccagacag
     1801 agtcatacat tttccgtaac gcctggaatg agaagaatta cggaaaagag acagactact
     1861 tcacacacct gaccggctgg gacagtgaca acaacgctgt cgactatggc ggaacactga
     1921 aggacgctgc tgtcacaggc aacggtacgt atacggtagg cgtcgatctc ggccccatgg
     1981 gtctgggttc tgaccagacg atccgtatgc tctatgtgtc caccgatata cccagtgttc
     2041 ttgttgataa aggatatgta acgatcagtg acgttaagac gtcgatagac ggcggaaaag
     2101 cccagacagc attcacggtc aataccgacg gcgattatgt ccagatcgat atactcaatg
     2161 aatacacttc gaccggaacg gaagccattt catacaagat gccgacaaag agcgtcacta
     2221 tcacatttac ggtttccggc ctgagcaagg atgccggatc agccggcgct tcagcttccg
     2281 gtcttccgaa ggcaggcgga gtgccgggcg aactcatgta tgtactgggc tgtgcagtaa
     2341 catcacttgg aatatacatg aaggcaagga agaagaaagt ctgacgccga aagggagggt
     2401 gcggtccccg aacaggggcc gcacctgcgg atcccttatg caagccgcct ttacgatata
     2461 tgatccaggc tttatcgttt aaggagaata aatgatatga ataacacggc gatacaaccg
     2521 aacgaactta ataccgcaaa agaggtcaag actttaaaac cgtcgacgat actgcatggc
     2581 atccttcgca taagttcact gattttatgc ctgtgcatgt tttttcccgg gctcaacccg
     2641 gcacgcattt cgaatctgat aaacaaaaat atttcattgt tcacgacggg gttttcgttt
     2701 tcaaacctta ccacaaggtt caacgccatg ttcaggcagg gcgcggtcaa taaatcgacg
     2761 ttcgtattgt tgagcgccgc atcggttgtc ataattgtcg gtgcgatcct ttgcgggatc
     2821 ggaggatgta tgtcgcttgg caacaaccgc atgaggtcgc gcgggatctg gttccccctg
     2881 gcaggagccg tcctggtatc agccggtctg tgcggtatat ataagtctta cctggaactc
     2941 caggcatcca ggggcgcgcg gcgggcacag gtagtgtttc cttctgtttt ctatatctat
     3001 gcaggccttg cggccgtttt gctcctggtc agccttataa tactcgtgtc cgagcggcgg
     3061 ctcgagaagg aagaaaaaat ggagatgccc gagaaatata ccctcttcct catgctgctg
     3121 cctataataa tcctggcgtt cgtcttttta tacctgcccc tgtacggatg gcgctattca
     3181 ttcttcgact acgaggcagg gggtacattg agccgcgaga atttcgtggg gttcaaatgg
     3241 tttacgatac tgttccggaa tcaggcgacg gtcaacgata tattgagagt cctgcgcaat
     3301 accctggcga tgagcgggct tggcatactg acaagctggg tgcctatggc atttgcgatc
     3361 ttcctcaatg aaatcaggag caaccgcttc aaacgcgtag tccagacagt gacaaccatt
     3421 ccgaatttta ttagttgggt gctggtatat gcgatcgcgc tcgctatttt cagtacggaa
     3481 ggctttttga attcattcct ggagcttttt aacgccaacc ggaatatcaa ctggctgatg
     3541 agttcggagc atacatggct caaaatgctc ctgtggggca tctggaaaag ccttggatgg
     3601 agcgccatcg tttatatcgc tgcaatagca ggtatagacc aggaattgta tgaagcggca
     3661 aaaatcgacg gcgccggccg attccagagg atctggcata taactgtgcc gggactcatg
     3721 ccgacatacc tggtgcttct gctgctcacg atagcgggag ctctcagcaa tggcctggag
     3781 caatacctga tgttccagaa cgcgaataac aaggacgtta tagaagtcct cgacctttat
     3841 gtttacaaca ttggcattac cggcggaagg atcccgcttt cgaccgttat cggaatgacg
     3901 aagtcattgg taagcatcgc tctgctgatg ttcgcgaaca gggtgtccaa gtcggttcgc
     3961 ggcgaaggga taatttagga ggtgcaggat gaacaactca acggagctca aacttaaaag
     4021 cgcccgtcgt tccggattcc gcagaagtcc gggggaagtc gcattcgatg tgataaacgt
     4081 tacggtgttc acgattttca tgctgctgtg catcttcccg ttttactacc tgtttatcaa
     4141 taccatttcc gacaacgacc tcgtactgaa gggagcgatt acatattatc ccgtggggat
     4201 aaacatatcg aactacattg ccctgaaaga ggtcaacgac ctcggcagcg cattcatcgt
     4261 gaccgtttcg aggacgatca taggaactgc gctgatggtg gctgccagcg ccctggcggg
     4321 ttatctcgtg acaaaaaaga agctttggaa acggagtttc tggtatcgct acctcgtcat
     4381 aacgatgtat ttcaacgcag gtcttatccc ctggtacatg aacatggtta tgctgggcct
     4441 gacggaaaac tatatggcgt atataatccc gggcatcgta gcgccgtata atataatcct
     4501 tgttaagacg tatgtcgagt cgatccccgg cgaactggaa gagagcgcat tcatagacgg
     4561 cgcatcgact tttacgatct tccggaagat catatggccg ctttcgaaac ctatcctggc
     4621 aacgatcgct gtttttggag cggtagggaa ctggaactca ttccaggact ctctgctgct
     4681 tatgaaaagc tcgccaaaac tgttcacgct gcagcaccgg ctgtactttt accttgttaa
     4741 ggcttccgac cttgagtcga tcatggccgg aggcacaggc acgaatgttg tactggaaaa
     4801 tactctgaat tcaaaagtag tcaagtacac gatcgcgatg gcgacaataa tccccatact
     4861 gcttgtttac ccgattatgc agaagtactt tgtcaagggt atcatgcttg gagccataaa
     4921 gggatgattt cggcgaacga tcacacaccg ggcatatggc ccggatatga aacatattaa
     4981 caatgaaagg gtgctctgaa tgaaaaagct gaaaggattc aaaaagatcg tatctactat
     5041 cctgagcgtt atgctgatcc tgtccatgct gttcatgatc accggctgcg gacaggacgg
     5101 cgggacggca gacggtgatg cggccgtaca gggaaccgac gggtcggcgt ctgatgcctc
     5161 aggcggccag gcatcggcag gcggagacgc ttcagcagat ggcacgagcg gcaacgccgg
     5221 cggaactacg gcagacggcc agcctgctgc aggagatgca aatgatgagt caggaacggg
     5281 attaactgaa gatcagccga tggtccagga agcggccggc agcgaagaga tagaagtagt
     5341 ggaaagtact gataaggcgg aaaagcccag tgaactgatc acgctggacg tatattcgca
     5401 gcttgccaac tggagcggga atcagatagg ctggtatgcc gcactgcttg aggacaaatt
     5461 caatgtcagg ctcaacatca taccggacgc cgaaggtaca tacgaaacgc tgattgagtc
     5521 aggcgacatg ggcgacatcg tcgtatgggg cgataacggg gccgaatata agaaagccgt
     5581 tcagctaggg ctcctttatg actgggaaga gggcgatctc tgcaagacct atgcgcctat
     5641 gatctggaac aactgtaaga tcgctcttga gacaaacaga aagatatcgg gggacggcca
     5701 tatttatggt atcggccaca gcgttgccct gagcaaggat gaccaccaga gcttcttcta
     5761 tacatgggat ctcaggtggg atctgtataa aaagcttggt tacccgaagg taaagaacct
     5821 cgacgactat ttcaatgtac tcaaacagat gaaagagatc tgccctaaag acgagaacgg
     5881 aagggaaaca tatgcagtat ccctctggcc tgactgggac ggcagcatgg taatgtatgt
     5941 aaaggctatg gctaccgcat actatggtta tgacgaactg gctgtcggcc tgtataatcc
     6001 cacaaacggc gaatactatg acgcacttat ggaaaacggt ccatacctcg aaatgctgaa
     6061 attcttcaac aggctttatc agaacggcct ccttgatccc aactccatga cccagacatt
     6121 tgaccagatg tctgataaag tcaaagccgg cggcacattc ttttccattt tcaactttgc
     6181 cggatcagcc tcctacaaca cgaccgagca catgaaacag ggaaagatga tgcttccgat
     6241 ggttcccgag gatgcttcgc cggctgttta cgggatgagc atgcttggcg gcaaccgtgt
     6301 ctggtcgatc ggtgcttata ccgaatatcc cgaactctgt atggagatcc tcaactacct
     6361 ctttacgccg gagggcgcca tgactatctg gtatggcctg ccggaactga tgtggtatta
     6421 tgatgacaac ggctatactc actttaccga ccttggcctg aaatgcaacc gcgatcctca
     6481 ttacgatctc ggcggcgtca catggaccag ccctgaaaca ggcaagacat acaagcttgg
     6541 cggcaatttc accgacggaa tgctgcagat caacaatacg acgtgggtaa tcgacacaaa
     6601 gaatccggat tccaacggtg agacattcaa caaggataca tggcgcagca tggcgggccc
     6661 tgcccagagc acgatcgaac aggattggcg tgactactac aaggtcacga ctgtcaatga
     6721 atacatgaag aagggcaagt acatggtagt acccggaaca tcgtattcag agccgaagcg
     6781 ttccgacgaa ctcgaactga tctggacaca ggtcacacag gcaatcaagc agtactcgtg
     6841 gcgtgctatc tatgcaaaga atgacggcga gttcaactac catgtacagc agatgatcaa
     6901 ggtctgcaaa gactatggtt acgaccaggt ccgcgaatgg agccgtcagc aggctgcaac
     6961 gcgttacaag ctgcagaagg ccgagaactg atcccctttc tccgatacgg gaaccggttc
     7021 cgtatcaaaa cctggatata gcgatgagac aggtatcccc cacttttata agtgggggat
     7081 acgctattaa cagcagatac tgttttgcct gcaaatacat ttttgctgat gtaccggcaa
     7141 gcattccggc cgtgatgata ctgttccggc cctgtaaggc aatgctgcaa tattccggtt
     7201 ccacctggca ctggcggggt acaggtttta tccagaccta cggtataggc tttgggcgaa
     7261 acctggg
//
LOCUS       DAOSCC010000123         7165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6706_length_7165_cov_4.053024, whole genome shotgun sequence.
ACCESSION   DAOSCC010000123 DAOSCC010000000
VERSION     DAOSCC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6706_length_7165_cov_4.053024"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..776)
                     /gene="fliP"
                     /locus_tag="PLG72_07430"
     CDS             complement(3..776)
                     /gene="fliP"
                     /locus_tag="PLG72_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249122.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOJ80665.1"
                     /translation="MIRMKRGLKIATALTIITLFSSDAYAETTFPFKFGFSVEPASTP
                     QEVSSSIQLLLILTVLSLAPAIIIMMTSFTRIIIVLSFLRNALGTQQMPPNQVLIGLA
                     LFLSLFVMTPVINEINTQAYEPFNKGEITQEQAFRNAENAMKGFMLKQFGDDEKELAF
                     FVSLAKIETPVRDPMDLPMTVVIPAFIINELTIAFKIGFLIYIPFLIIDMVVSSTLMS
                     MGMMMLPPVMISLPFKVLLFILVDGWNLITQTLVSSFIT"
     gene            complement(751..1284)
                     /locus_tag="PLG72_07435"
     CDS             complement(751..1284)
                     /locus_tag="PLG72_07435"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOJ80666.1"
                     /translation="MGTIDYILRIILFLIGFGALLFLTYVTTRYIGRKQIRSMTGRNI
                     SIIETVMLGPDKRLHLVRAGKSYVLIATTSRSVEYLATVELDGNAEATAAEEDNGSRF
                     DFRSVFEKYSGIYRAKAMKNTNDEKPVPSGDEREEPAFRKNLEKLRIILKRNLENGTG
                     NDKNGVDDTNDKDETRS"
     gene            complement(1357..1719)
                     /locus_tag="PLG72_07440"
     CDS             complement(1357..1719)
                     /locus_tag="PLG72_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ80667.1"
                     /translation="MGKTVLIVDDAAFMRMMIRDILTKNGYEVVGEAENGGRAIEKYK
                     ELLPDLVIMDITMPEVNGIEAVREIRKLNPDSQVIMCSAMGQQAMVIESIQAGARDFI
                     VKPFRAERVIEAVKKVLG"
     gene            complement(1827..3026)
                     /gene="fliY"
                     /locus_tag="PLG72_07445"
     CDS             complement(1827..3026)
                     /gene="fliY"
                     /locus_tag="PLG72_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOJ80668.1"
                     /translation="MGDMLSQAEIDALLKGVIPSGPSDADLDPQEIDALGEIGNISMG
                     TSATTLFALLGQKVTITTPKVSVCTWKEISTQYSQVHVGVRVEYTKGLIGTNLLILKE
                     HDVKVITDLMMGGDGSNTEGSLTELHLSAISEAMNQMVGSSSTSMSSMFDKRIDISPP
                     KAFIFNVENMNENTLMDDNEKVVKVAFKMVIGDLVDSEIMQIIPISFAKEMVGALLSM
                     ETDSGTYKLPDEVSAGQAETAPVREASHSAPGPAAANFNDQPQTAYQPFSQPQPAQRS
                     YHGAPQYDHGFVEHEPRQHREPVNVQPAQFQSFEEDRIVLDRHNINLIMDVPLQITVE
                     LGRTQRLIKDILEFSPGSIIELDKLAGEPVDILVNGKQIAKGEVVVIDESFGVRITDI
                     AAPSKRL"
     gene            complement(3026..4012)
                     /gene="fliM"
                     /locus_tag="PLG72_07450"
     CDS             complement(3026..4012)
                     /gene="fliM"
                     /locus_tag="PLG72_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOJ80669.1"
                     /translation="MGDILSQNEIDDLLKALSSGEINAHEIQTTTQKKKVKTHDFRRA
                     SKFAKDHIKTLNIIYDNYARLVTNFLTGYLRTLVQVDVVSVEALPYSDFSNSVANPVI
                     MAIVDFAPLSGSIIFEIEPHIAFALVDRILGGKGSSMERIREFTEIELAIIERIVIQM
                     LNLMREPWENVLSIRPRLDKIETNAQFAQIVAQNETVALITLSARVGEVDGMMNICIP
                     HMVVEPIVSKLSTRFWFSTVEKEATPEMKRDIERGVQNTSVPVKAVLGRTTVTVRDVL
                     ELQVGDVLALDTGVNDELEVRVGDLLKFYGIPGVKKNRVAVRITKAVKKEEE"
     gene            complement(4052..4576)
                     /locus_tag="PLG72_07455"
     CDS             complement(4052..4576)
                     /locus_tag="PLG72_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOJ80670.1"
                     /translation="MESRSTFFILIVIVAVLALSLAALAGYLLIVQGSSDGGGNAQQN
                     GVGEGSGVAPKEEDLLKIPLYDGAKYFTLKKASPNDSSIIQVNVTLKCFKELKKNRKV
                     VVEELIDAKKEEIQELVVRFFLKRTIDEIKDVEMLDKAKDELTGQINSLLNEDLNKPE
                     DVVYRVIFSEWLFQ"
     gene            complement(4580..4792)
                     /locus_tag="PLG72_07460"
     CDS             complement(4580..4792)
                     /locus_tag="PLG72_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOJ80671.1"
                     /translation="MIKLTRLNGSVYVLNSNLIETVESTPDTVITTVDGKRYVCRESV
                     DEVIRKVIEFEGNIRFHADTQKTEGA"
     gene            complement(5055..6401)
                     /locus_tag="PLG72_07465"
     CDS             complement(5055..6401)
                     /locus_tag="PLG72_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOJ80672.1"
                     /translation="MMRSMFSGVSGLRAHQTKMDVIGNNVANVNTVGFKSGRVTFQEI
                     FSQTLQGASAPDPVTGRGGTNPMQIGLGLGVGAIDTITTRGSVQRTDNITDLSIEGDG
                     FFICKGGSSDTFKFTRAGNFTIDLLGNLVTSSGMNVYGWQELKNDGSGEFDTDRPIEP
                     INIYSDAYRNKRIISAKNTSMVQLAGNLDAAMPTSSGPSETGGTGETGETGETGTNQD
                     DNILLVPMTVYDKLGNAYKIEVRLWKTGASTWQWDIPSSSGVYNVSGASGTLNFTPEG
                     KLNQAPNSPPIKIQFTPDRSKGTDSFDIELDFSKITCYAADNSVAPEFVDGYTSGSLV
                     SISIGSDGVITGIYDNGQQQALGLIALSYFGNPAGLQKVGDNMYLPTPNSGDYKAFRP
                     GSGSTGMLNPGTLEMSNVDLSKEFTEMIITQRGFQANSRIITTSDEMLQELVSMKR"
     gene            complement(6483..6881)
                     /locus_tag="PLG72_07470"
     CDS             complement(6483..6881)
                     /locus_tag="PLG72_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOJ80673.1"
                     /translation="MVIDNTGNRINRTVITGIPPARTDRPESRKDTAAVSFDSILRQK
                     QKENEEIKFSKHAEYRLRSRNISLTPSQKDRMMEAVAKAESKGVRDTLVLMDDLAFVV
                     NIRNRTVITAVNSSELKENVFTNIDGAVII"
     gene            complement(6871..>7165)
                     /locus_tag="PLG72_07475"
     CDS             complement(6871..>7165)
                     /locus_tag="PLG72_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80674.1"
                     /translation="KDSSTGKRVPVTAIITDLKFDNKGGIKAVLNDVHVPLESITNIK
                     KQPEGNSAEGGNETGAAEETEDGSRQDGENGTDTVGNSGETSSDGQAGDDNGN"
BASE COUNT         1542 a         1953 c         1522 g         2148 t
ORIGIN      
        1 gcctatgtaa taaaagacga aaccaacgtc tgtgtaatca ggttccagcc gtccaccagt
       61 ataaacaaga gtaccttgaa cggcagcgat atcattaccg gcggaagcat catcatgccc
      121 attgacataa gcgtgctcga aactaccatg tcaatgataa gaaaaggtat gtatatcaga
      181 aatcctatct tgaacgctat tgtcagttca tttatgataa aggcaggtat tacgacggtc
      241 attggcagat ccatcggatc cctgaccggc gtttctatct ttgcaaggga tacgaaaaaa
      301 gcaagttcct tctcatcatc tccaaattgc ttcagcatga accccttcat ggcattttcc
      361 gcattcctga acgcctgctc ctgtgttatc tcacctttgt tgaagggctc atacgcctgc
      421 gtatttattt cgtttataac cggagtcatt acgaaaagcg acaaaaacaa agcaagcccg
      481 atcagaacct gattaggcgg catttgctgt gtcccgagcg cattcctcag gaaggaaagc
      541 actattatta tccttgtgaa agaggtcatc attatgatga tggccggagc cagagacaat
      601 acggttaaga taagcagaag ctggatactt gacgaaactt cctggggagt ggatgccggt
      661 tccacactga aaccgaactt gaagggaaaa gtggtttcag cataggcgtc tgagctgaaa
      721 agagttatga ttgtcaatgc tgttgcgatc ttaagacctc gtttcatcct tatcattagt
      781 atcgtcaacc ccgtttttat cattaccggt accgttttcc aggtttcttt tcaaaatgat
      841 cctgagtttc tcaagattct ttctgaaagc gggttcttca cgttcgtccc ctgatggtac
      901 cggtttttca tcattcgtgt tcttcatggc ctttgctctg taaataccgc tgtacttctc
      961 gaaaacagac ctgaaatcaa aacgcgagcc gttgtcttct tcagcagcag ttgcttctgc
     1021 atttccatca agctccaccg tagcgaggta ttcgacggat ctcgatgtcg tggctatcag
     1081 cacgtagctt tttcctgccc tgacaaggtg cagtcttttg tcagggccaa gcatcaccgt
     1141 ctcgattatg ctgatatttc tgcctgtcat cgacctgatc tgcttgcgtc cgatatacct
     1201 ggtagtgacg tatgtaagga acagcaacgc gccgaatccg atcaggaata aaattatcct
     1261 cagtatgtaa tcaatagtcc ccatcgtaat cccccggcct gcaagcggcc tgctccactg
     1321 cccgcccggc cttttccggc atcttttgca ggcatttcag cccagcactt tctttacggc
     1381 ttctatcact ctttcagccc ggaacggctt aacgataaaa tctcttgcac ctgcctgtat
     1441 cgactcaatg accatggcct gctgtcccat ggccgaacac ataatcacct gggaatcagg
     1501 attcagcttc cggatctccc tgactgcctc gataccattg acctcgggca tcgtgatatc
     1561 catgatcacc agatccggga gcaactcctt gtatttttct atggcacgtc ccccgttctc
     1621 cgcttctccg accacttcat acccgttttt cgtcaatatg tctcttatca tcatcctcat
     1681 aaatgcagca tcatcaacaa tcaagacggt ttttcccata aattctctcc ccttatgtgt
     1741 agtgagtcga aaattatgtt attttactgt tattcatgta tatttatgtt aacatttcgc
     1801 acatttataa tcaaggcatt tttttcttac agccgcttgc tgggggctgc aatatcggtt
     1861 atcctgacgc cgaagctttc gtcgatgaca actacttctc ccttggctat ctgttttccg
     1921 ttcacaagta tgtccaccgg ttcgccggcc agtttgtcaa gctctataat ggatccgggg
     1981 ctgaactcga gtatgtcctt gatcagcctt tgcgtccttc ccagttcgac tgtaatctga
     2041 agcggcacat ccatgatcag gttaatgttg tgcctgtcca gtacgatccg gtcttcctcg
     2101 aaactctgga actgggcagg ctgtacgttg acaggttccc tgtgctgcct cggttcgtgt
     2161 tccacgaatc catggtcata ctgaggagcg ccatgatatg accgctgcgc cggctgtggc
     2221 tggctgaacg gctgatatgc cgtctgcggc tgatcattga aattcgcggc cgccggtccc
     2281 ggtgccgaat gagatgcttc tctcacgggc gcagtttccg cctggcctgc cgatacttca
     2341 tccggcagct tgtatgtacc gctgtcggtc tccatgctca gcagggctcc aaccatctct
     2401 ttcgcgaaag agatcggtat aatctgcatt atctcgctgt ccaccaggtc gcctataacc
     2461 atcttgaaag ctactttcac gaccttctca ttgtcatcca tgagagtgtt ttcattcatg
     2521 ttctcaacgt tgaatataaa tgctttcgga ggtgaaatat ctatcctttt gtcgaacatg
     2581 gaagacatgg atgtggaaga tgatcccacc atctggttca ttgcttcact gatggcgcta
     2641 aggtggagct ccgtaaggct gccttcggtg ttcgatccgt cgcctcccat catcaggtcg
     2701 gttatgacct tgacgtcatg ttccttcaat atcagcaggt tcgtaccgat caggcctttt
     2761 gtatactcga cccttactcc tacatgcacc tgtgaatact gtgtagatat ttctttccat
     2821 gtacagaccg acaccttggg agtggttatc gtcactttct gtccaagaag cgcaaacagt
     2881 gtagtagctg aagtccccat gcttatattg cctatctcgc caagagcgtc tatctcctgg
     2941 ggatcaaggt ccgcatcgga cggtcctgaa ggaattacac ccttcagcaa tgcatctatt
     3001 tcagcctgag ataacatatc acccattact cttcctcctt cttcactgcc tttgtgatcc
     3061 tgactgccac cctgttcttc tttacgcccg ggatgccata gaatttcaga agatcaccga
     3121 cgcgcacttc cagttcatcg ttcacaccgg tatccagcgc aagcacatct ccaacctgca
     3181 gttcaagaac gtcccttacc gttaccgtcg tcctgccgag tactgctttt accggaacgg
     3241 atgtgttctg tactcctctt tcgatatccc gtttcatctc aggcgtggct tctttttcca
     3301 cagtcgaaaa ccaaaacctg gtactgagct ttgaaactat cggttcaacc accatatggg
     3361 gtatgcatat attcatcatt ccgtcgactt cgccgacacg cgcgctcagc gttatcagcg
     3421 ccaccgtttc gttctgcgcg actatctggg cgaactgcgc attggtttct atcttgtcca
     3481 gccttggtct gatcgataag acattttccc acggctcacg catcaggttc agcatctgga
     3541 tcactattct ctcgattatg gcaagttcta tctccgtaaa ttcccttatc ctttccatgg
     3601 aagacccttt tccgccgagg atcctgtcca ccagcgcaaa tgcgatatgc ggctcaatct
     3661 cgaatattat ggacccggac agcggagcaa agtccactat tgccattatg acaggattcg
     3721 ctaccgagtt gctgaaatca ctgtacggaa gcgcttctac ggatacgaca tcgacctgta
     3781 ccagggtcct caggtagcct gtcaggaagt tcgtaaccag cctcgcgtag ttgtcgtata
     3841 ttatgttgag ggtctttata tgatcctttg caaatttgct ggctcttcgg aaatcatggg
     3901 tctttacctt ttttttctgc gtcgtggtct ggatctcatg cgcgttgatc tcacctgagc
     3961 ttaacgcttt caaaagatcg tctatttcat tctgcgaaag gatgtccccc ataaaatcac
     4021 ctcccgtctc agaccgttat cactgaacgt actactggaa cagccattcg gaaaaaatca
     4081 ccctgtacac gacatcctca ggcttgttca gatcttcgtt cagcaggctg ttgatctggc
     4141 cggtaagctc atctttggcc ttgtcgagca tttccacatc cttgatctcg tcaatggtcc
     4201 gtttcaggaa gaaccttacg acaagctcct gtatttcttc cttttttgca tctataagct
     4261 cttcgacaac tactttcctg ttctttttca attctttgaa gcatttcaga gttacattca
     4321 cctggattat ggacgagtcg ttcggactgg cttttttcag tgtaaaatac tttgcgccgt
     4381 cgtaaagcgg gatcttcaga agatcttcct ctttcggcgc cacaccgctt ccttctccga
     4441 caccattctg ctgggcatta cctccgccgt cagagctgcc ctgcacgatc agcaggtatc
     4501 cggcaagagc ggcaagtgaa agagcgagaa ctgcaacaat gactatgaga atgaaaaatg
     4561 tgctcctgga ctccaattct tatgccccct ctgtcttttg tgtgtctgca tgaaacctga
     4621 tgtttccctc gaattctatt accttcctga tgacttcatc gacagattcc ctgcagacgt
     4681 atctttttcc atcgactgtg gttatgaccg tatcaggcgt cgattccaca gtttctataa
     4741 gattgctgtt aaggacataa accgaaccgt ttagtctggt cagtttaatc ataatatttt
     4801 tgctccaaaa actcaatagt ttttcataaa atggcagttc ccgcgctttt tcatgaaaaa
     4861 ttaccgagtt tttccccttt cttcttatat ttccggaagc gctccttctg atcagcatac
     4921 catatacgaa atcacttata tttattgccc gttcagaagg agcgccccgg cagcacccca
     4981 aaccgtctct attcgtttgc cgcctgcttt atctgtcgtt catgcgatca tgtcagccgc
     5041 tgctgccacc ggaattatct cttcatgctt accagttcct gcagcatctc atccgatgtt
     5101 gtgatgatac ggctgttggc ctggaagcct ctctgcgtga tgatcatctc tgtgaactcc
     5161 tttgacaggt cgacattgga catttcaagc gtacccgggt tgagcatccc ggtactgccg
     5221 gatccgggcc tgaacgcctt gtagtctccg gagttgggcg taggcagata catgttgtcg
     5281 ccgaccttct gcaggcccgc cgggttcccg aaatatgaca gggctatgag cccgagcgcc
     5341 tgctgctgtc cgttgtcata tattccggta attaccccgt cggagccgat gctgatcgaa
     5401 accaggcttc ctgatgtata tccatcgaca aactccggcg cgacagagtt atctgccgca
     5461 taacaagtta tttttgagaa gtccagttct atgtcaaatg aatccgtgcc cttcgatcta
     5521 tcaggagtaa attgaatttt tataggtggc gaatttggcg cctgattcag ctttccttca
     5581 ggtgtaaagt taagtgtccc gctggcacct gagacattgt acacaccgct ggaagaaggt
     5641 atatcccatt gccatgtcga tgcacctgtt ttccataacc gcacttcgat cttgtaagcg
     5701 tttcccagct tgtcatatac ggtcatcggc accagcaaaa tgttatcatc ctggttagtg
     5761 ccagtttcgc cagtttcacc ggtttcaccc gttccgccgg tttcgctggg tccgctgctt
     5821 gtcggcatcg ccgcgtccag gttgcctgcg agctgtacca tggaggtgtt cttggctgaa
     5881 attatcctct tgttcctgta tgcatcagaa taaatgttta taggctctat cggcctgtcg
     5941 gtgtcgaatt ccccgctgcc atcgttttta agctcctgcc atccatatac attcatcccg
     6001 ctgctggtta caagatttcc aagcaggtcg atggtaaaat ttcctgccct ggtgaattta
     6061 aacgtgtcac tggagccgcc tttgcagatg aagaaaccgt caccctctat cgacaggtca
     6121 gtaatgttgt ctgtcctctg cacgcttccc ctcgtagtaa ttgtatcgat cgcacccact
     6181 ccaaggccca gtcctatctg catcgggttc gttccgcccc tgccggtaac cgggtcaggt
     6241 gcgctcgctc cctgaagcgt ctggctgaat atctcctgga atgtcactct tcctgatttg
     6301 aaacctaccg tattgacatt tgcgacgttg ttgcctatta cgtccatttt ggtctgatgt
     6361 gccctgagtc ccgacacacc ggaaaacatt gatctcatca taaattcatt tcgacctcct
     6421 tcagaactta tacatcccgt ccgtcgttca gggatgccca gcctcccgaa agggtccggc
     6481 tgtcagatta tcactgctcc gtctatattc gtgaatacat tctccttcag ttcactgctg
     6541 tttactgccg ttatcaccgt cctgttcctt atattgacca caaatgcaag atcgtccatc
     6601 aaaacaagcg tatcccttac tccttttgat tcagcctttg ccaccgcttc catcatcctg
     6661 tccttctgag aaggcgtcag gcttatgttc ctggaccgga gccggtattc cgcatgcttg
     6721 gagaatttta tctcttcatt ctccttctgt ttttgccgca gtatactgtc gaaacttacg
     6781 gctgccgtat cctttctgct ttccggcctg tctgtcctgg ccggcggaat gcctgttatg
     6841 acggtcctgt taatgcggtt gcctgtgttg tcaattacca ttatcgtcac ctgcctgtcc
     6901 gtcggacgaa gtttctccgc tgttgcctac cgtatcggtt ccattttctc cgtcctgccg
     6961 tgatccgtct tcggtttctt ccgcggcgcc tgtttcattt ccgccttcgg ctgaatttcc
     7021 ttctggctgt ttttttatgt tcgttatact ttcaaggggc acatgcacat cgttcaggac
     7081 cgctttgatc ccgcctttgt tgtcgaattt caggtcggta atgatggcag tcacgggaac
     7141 cctctttccg gtagaactgt ctttg
//
LOCUS       DAOSCC010000124         7061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6839_length_7061_cov_4.235512, whole genome shotgun sequence.
ACCESSION   DAOSCC010000124 DAOSCC010000000
VERSION     DAOSCC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_7061_cov_4.235512"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLG72_07480"
     CDS             <1..603
                     /locus_tag="PLG72_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ80675.1"
                     /translation="ADEKYIAGLRRLCDEKGILLIFDEVQTGIGRTGKMFAYQHYGIE
                     PDIMTLAKGIGGGFPLSAMLTKEKFNIFDPGDQGGTYTGQPLAMAVGLAVVKEVLSRD
                     LPARAAIMGDYITERLKELSEKYPIRNIRGKGLLVAFDLPDPVGEKVVSACLDEGLIL
                     NSPRPSTIRLMPPLIVMKQHVDEMLDILSKVLDRICYSGR"
     gene            complement(1140..1475)
                     /locus_tag="PLG72_07485"
     CDS             complement(1140..1475)
                     /locus_tag="PLG72_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ80676.1"
                     /translation="MKKLWKCSVCGYVHAGDEAPDACPKCGAPKDKFVGLSDEEAEKI
                     NKSERTNDIHMEIVVLAERIMALAQEGIDIDLDPPCVALFKAAVKEARVIKQRSKAEM
                     EGHMKKGKW"
     gene            complement(1685..2782)
                     /locus_tag="PLG72_07490"
     CDS             complement(1685..2782)
                     /locus_tag="PLG72_07490"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOJ80677.1"
                     /translation="MRKRLALCLVMAALAAAVCGCGDKSGGQEIQPVVVETALPEPFA
                     DTGDSSAEQSPENQPEPGDEPAVPAEDDYTLPFTGVRPMAVMIDNQGSRVLPQGGLDK
                     AQIVYEVIVEGGLTRLMPVFWGTEPEMIGPVRSARHYFLDFSMEYDAIYVHYGGSPQA
                     GTDIRKLKVDEIDGIALGPEYFWDLTTDRNNWQDSYTSMEKLKIYAEKRRFRDTTDAG
                     FPLRYNGPGDDLVPEGGDDAKTVSLKYPSMSVRYEYDSGTGTYKRFRYDKEHMERVSG
                     KQFEAKNIIVLYMRNWTIEGDREGRQDMQTTGTGKGYFITCGKAYKIKWSKETRSSQT
                     KYTYENGEPIVLNRGQTWIQVMPVNAKVTIE"
     gene            3064..3756
                     /locus_tag="PLG72_07495"
     CDS             3064..3756
                     /locus_tag="PLG72_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ80678.1"
                     /translation="MTPQDIALGTRLELEMIDDNGERIGNVYVSQLLEHQEDGTMVIS
                     APISGSRVIYIPDGITIRLTFIDPIQGLLGFTAVTGYKEYRGKVAVIIVKPGTEISKI
                     QRRMHYRLETVLDAAIRPANADGTAEAAEKADPVKVCTKNISGSGVCIISETDIPKNT
                     MLEMELDLTDGTRIKTRGVVVRSQEVEARKGRNYEVGVYFTDISKKDQDRLIRYIFEQ
                     QRILLKKDKEKE"
     gene            complement(3872..4168)
                     /locus_tag="PLG72_07500"
     CDS             complement(3872..4168)
                     /locus_tag="PLG72_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOJ80679.1"
                     /translation="MDAERDDIVVLIDEDGEEVEFEHIDTIELNGNEYVVLVPLEEEE
                     DDGQEEEEVVILRVEHTEDGEDTFVTIEDDDELEEVFDEFQSRLEEEFEDDDED"
     gene            complement(4215..4631)
                     /gene="ruvX"
                     /locus_tag="PLG72_07505"
     CDS             complement(4215..4631)
                     /gene="ruvX"
                     /locus_tag="PLG72_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ80680.1"
                     /translation="MRIMGIDFGDSRIGIAVSDPMGWTAQGLETIERKGPMEQDAERI
                     RQLAKQYGVGKIVIGLPKNMNGTIGPSAEKAIEFGELLARTTGLEVVQWDERLTTVAA
                     NRLMHEVGMKTSKKKKSVDRIAAVFILQGYLDSMRN"
     gene            complement(4710..5657)
                     /locus_tag="PLG72_07510"
     CDS             complement(4710..5657)
                     /locus_tag="PLG72_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ80681.1"
                     /translation="MEYVELGKTGLRVSRLCFGALVIGPLQKNMPVRDGAAVIRAALE
                     RGVNFIDTAELYGTYGHIREAVKGLGEKPVVATKSYAYTAGGAAESVEKARKELDMDV
                     LDIFMLHEQESRLTLKGHREALEYYLNEREKGRIRAVGVSTHNIEVVRAICDMPEIDV
                     VHPLVNITGIGIGDGSTGEMLEAVKAAYDRGKGVYSMKPLGGGNLLRSYDECMDFVLG
                     LPYVHSIAVGMQSADEVEMNILKFEGREIPARLKEAAALRKKRLHIDYWCTGCGKCAE
                     RCNQKALSVTGGKAVPDPGKCVLCGYCSGACPQFAIKIC"
     gene            complement(5821..6075)
                     /locus_tag="PLG72_07515"
     CDS             complement(5821..6075)
                     /locus_tag="PLG72_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOJ80682.1"
                     /translation="MAGSETMMFKMDKDKENEARDILFTVYQALNEKGYNPINQLVGY
                     ILSGDPTYITNHLNARSVIRRLERDELLEEIVRFYLENKK"
     gene            complement(6205..>7061)
                     /locus_tag="PLG72_07520"
     CDS             complement(6205..>7061)
                     /locus_tag="PLG72_07520"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOJ80683.1"
                     /translation="VQEGCNQFCSYCIIPYARGPIRSRPAEEVISEVHKLVEAGFREV
                     VLTGIHIASYGIDLGQIRLPDLIRMVHDVDGIERIRLGSLEPTMITKEFVAEAAGLEK
                     LCPHYHISLQSGCDETLKRMNRKYTTAEYAKAVELLREEIPDVSVTTDVMVGFPGETD
                     EEFETTYMFLEKIRFAKMHVFKYSPRKGTPAARHKDQVDGRVKEERSDRLIELSNRCQ
                     LEFNRNFLGRVMPVLYEQEDNDMKGWYEGLTPNYIRVLSKGDPGISGNILPTRLLEAE
                     NDFILGEI"
BASE COUNT         1627 a         2005 c         1601 g         1828 t
ORIGIN      
        1 gcagacgaaa agtatatcgc gggtctgcgt agactgtgtg atgaaaaggg cattttgctg
       61 atctttgatg aagtgcagac aggaataggc aggacaggga aaatgtttgc ctaccaacat
      121 tacggcatcg agcccgacat aatgacgctt gccaagggaa taggcggagg tttcccgctc
      181 tccgcaatgc tgacaaagga gaagttcaac atcttcgatc ccggcgacca gggcgggaca
      241 tataccgggc agcctcttgc catggctgtc gggctggctg tcgtaaaaga agtgctgtcc
      301 agggatcttc cagccagagc cgcaataatg ggagactata taacagagcg gctgaaggag
      361 ctgtcagaaa aatatccgat caggaatata cgtggaaaag gcttgctcgt cgcgttcgac
      421 ctgccggatc ctgtcgggga aaaagtggtg tctgcctgcc ttgatgaagg cctgatcctg
      481 aattcgccca ggccctcgac tatcaggctc atgcctcctc tgatcgtaat gaagcagcat
      541 gtcgacgaaa tgctggatat actgtcaaag gtactcgaca ggatctgtta tagcggccgg
      601 tagttaaagc agcaagggga accacagaag caatcaggga aaacacggaa gcggtttggg
      661 gaacatggca gcagtgagtg aactcatgga agcagtaagg gaaaccatgg aaacattcag
      721 gaagcttgca gaaacagtaa tgtaaaccac ggaagcagta aaggaactcc tggccgccgc
      781 actaaaagca tttccccgaa gtgtttactc aaagttttcc ctcgagtatt tccccgggaa
      841 ataaaacctg ggtgtacaaa aaacgaattg ttgtgtgtta aagtcgcttg tgtatggata
      901 aatatcagta atccagttac cgtaatgtgg tatttatggg cattatggta atctattcac
      961 aaagaataaa ctggaatgtt ctattatgtt aacccaacaa ttcgaatttt gtacacccag
     1021 gtctgcgttt gctgcactat cactatgctg ctgcaacacc actgcagcta tatttatgcc
     1081 gctgtatcgc cactgcggct atgttgccgt ggcttccctg ccgctatgga cctcccggat
     1141 caccacttgc cctttttcat gtggccttcc atttccgcct tggatctctg cttgatcacc
     1201 cttgcttcct tgacagcggc cttgaacagc gctacgcatg gcgggtccag atcgatgtcg
     1261 atgccctcct gggccagtgc catgatcctt tctgccagta cgacgatctc catgtggatg
     1321 tcatttgtcc tttccgactt gtttatcttt tcagcctctt catcgctgag tccaacaaac
     1381 ttgtctttcg gcgcgccgca tttcgggcag gcatcaggcg cctcgtcccc tgcgtgtaca
     1441 tatccgcaca cactgcattt ccagagtttc ttcatcagcc atcatccttt cataaatatt
     1501 gggaatctat attggacatc agttttatat atacccgaaa agcagtccgg ttaacatgac
     1561 gattccaaaa aatcacacct gtaaaatcat tgacggcaca accgggccat gcctttgggg
     1621 ctgactgagc cgaaggaccg gctccggtaa aatccgcgac acacacctgc agtattatcc
     1681 gctttcactc aatcgtgact ttcgcattga caggcattac ctggatccag gtctggccgc
     1741 ggttcagcac gatgggctcc ccgttctcgt atgtatattt cgtctgggag cttcttgtct
     1801 ccttagacca ctttattttg tatgccttgc cgcatgtgat gaaataccct ttccctgtcc
     1861 ctgtggtctg catgtcctgc cggccctcac gatcgccttc tatggtccag ttgcgcatat
     1921 acagcactat tatgtttttt gcttcaaact gctttcccga gaccctttcc atatgctcct
     1981 tatcatacct gaaacgctta tatgtcccgg tcccgctgtc gtattcatac ctgacggaca
     2041 tggacggata tttcagagaa acggtttttg catcatcgcc gccctcaggg acaaggtcat
     2101 ctcccggccc attgtatctt aacgggaatc ccgcatcggt ggtgtcgcgg aacctccgct
     2161 tttccgcata tatcttcagt ttttccatgg aagtatatga atcctgccaa ttattccgat
     2221 ctgtggtcag atcccagaaa tactcagggc caagagcgat accgtctatc tcgtcgacct
     2281 tcagcttcct tatgtccgtg cccgcctggg ggctcccgcc gtaatggaca tagatggcgt
     2341 catattccat actgaagtcg aggaaataat gcctcgcact tctgacagga cctatcatct
     2401 caggctcggt accccagaat accggcatca gccttgtgag cccgccctcc actatgactt
     2461 cgtaaactat ctgcgccttg tccaggccgc cctgaggcag cactcttgag ccctggttgt
     2521 ctatcatcac ggccattggc cttacacctg tgaacggcag tgtatagtcg tcttccgccg
     2581 gtaccgccgg ttcgtctcca ggttcgggct gattttccgg cgattgttcc gcactgctgt
     2641 ctccggtgtc tgcaaaaggc tccggcaaag ctgtttcaac aactacaggc tgtatctcct
     2701 gtccacctga tttatccccg catccgcata ctgctgccgc aagcgctgcc atcaccagac
     2761 acagggcaag tcttttccgc atttgtttcc ctccaggatc attagtcgga tattatataa
     2821 attctatcca atgttcacaa ttcctcttaa gatttgaatt ttgcctgccg aaatttattg
     2881 caggggctta tgccgaacac ggatgagcga tacggcatca aaactgttag aagtatgacg
     2941 aaacagggaa ataaaaataa gggtacgact gtagacagat aacagcatcg cctgaggatc
     3001 gatcccaggc agggatctct gacagaaacc atcatccgtc aagacacgac aggagaaatg
     3061 cagatgacgc cacaagatat agcgttaggg accaggcttg aacttgagat gatcgacgat
     3121 aacggtgaaa ggatcggaaa cgtatacgtc agccagcttc ttgaacacca ggaggatgga
     3181 acgatggtga tttctgcccc gatctctgga tccagggtga tctatattcc ggacggaatt
     3241 acgatccgcc ttaccttcat cgatccgatc cagggactgc tcggtttcac cgcagtgacc
     3301 ggctacaagg aatacagggg aaaagtcgcc gttatcatag taaaacccgg tacggaaatc
     3361 tcaaagatcc agcggaggat gcattacagg ctggaaacag tgcttgatgc ggccatccgg
     3421 cctgcgaatg ctgacggaac tgcggaagca gcggaaaaag ccgaccctgt gaaagtatgt
     3481 accaaaaaca taagcggatc gggtgtctgc ataatcagcg aaacagatat cccgaaaaac
     3541 accatgctcg agatggaact tgacctcacc gacggcactc ggatcaaaac caggggcgtc
     3601 gttgtcagga gccaggaagt agaggcaaga aaaggcagaa actacgaagt aggcgtctat
     3661 ttcaccgata tcagcaaaaa agatcaggac aggcttatcc ggtacatctt cgagcagcag
     3721 cggatacttc tgaaaaagga caaggaaaag gagtaataaa taatcgctga cggcgataat
     3781 gtatcccggg aaaaatggag aaccggccga agggaagcaa aaagccattg gatccgtcca
     3841 atggcttttt attcagactc tgaaaacact ttcagtcttc atcatcatct tcgaactctt
     3901 cttccagcct cgactggaat tcatcgaaca cttcctcgag ttcgtcgtcg tcctctatcg
     3961 tcacgaaagt atcctctccg tcttcggtgt gctcgaccct gaggatcacg acctcttctt
     4021 cttcctggcc gtcgtcttcc tcttcctcga gcgggaccag tactacatat tcgttcccgt
     4081 tcaattcgat agtatcgata tgttcaaatt cgacttcttc accgtcttcg tcgatcagga
     4141 caactatatc gtctctttct gcatccatgg cgatcacttc ctttcctata ctgccgtacc
     4201 atattataat aaccttaatt cctcatactg tcaagatatc cctgcaatat gaagaccgca
     4261 gcgatcctgt caaccgattt cttttttttc gaggtcttca tgccgacctc atgcatcagg
     4321 cggttggctg ccacggtcgt cagccgctcg tcccactgca ccacctcaag cccggtggtc
     4381 ctggcaagaa gctcgccgaa ctcgatggcc ttctcggcgc tgggccctat ggtcccgttc
     4441 atgttcttcg gcaggccgat gacgatcttg ccgacaccgt actgtttggc cagttgcctg
     4501 atcctctcgg catcctgctc catcgggcct ttccgctcta tcgtttccag cccctgtgcc
     4561 gtccatccca tcggatcgct gacagcgatg ccgattctac tgtctccaaa atctataccc
     4621 attattcgca taatgtcctc ctggtatccc ggactgtcca tcattcgcca atggaccgcg
     4681 atatggcgtt ccgggaaaag tatccgtcct cagcaaattt taatggcaaa ctgagggcat
     4741 gcgcccgaac aataaccgca cagaacacat tttccgggat ccggcaccgc tttccctccg
     4801 gttactgaaa gtgctttctg attgcatctt tcagcacatt tgccgcagcc cgtgcaccaa
     4861 tagtctatat ggagccgctt tttcctaagc gctgcggcct ctttcagcct tgcaggtatc
     4921 tcacgtcctt cgaacttgag gatgttcatt tccacttcat cggcgctctg catcccgaca
     4981 gcgatggaat ggacataagg caggccgagc acgaaatcca tgcattcgtc atatgatctc
     5041 agaaggttgc cgcctccgag aggcttcatg ctgtaaacgc ccttcccgcg atcataggcc
     5101 gctttgacgg cttcgagcat ctcccctgta ctcccgtcgc ctataccgat ccccgtgatg
     5161 tttaccagcg ggtgcacgac atctatctct ggcatatcac aaattgccct gaccacctct
     5221 atattgtggg tcgacacgcc gacagccctt atcctgcctt tctcacgctc gttgaggtaa
     5281 tattccagcg cctcccggtg ccctttgagc gtcagcctgg actcctgctc atggagcatg
     5341 aaaatgtcca gcacgtccat atcgagctct tttctcgctt tctcgacact ttcagccgca
     5401 cccccggccg tatatgcata tgatttcgtc gctaccacag gtttttctcc gagccctttc
     5461 accgcttccc ggatatgccc gtaggtgccg taaagctctg cggtatcgat gaaattgact
     5521 cctctctcca gcgctgccct tatgacagcc gctccgtccc tgaccggcat gtttttctgg
     5581 agcgggccga tcaccagcgc tccgaaacac agccttgaga cccttagacc ggttttgccc
     5641 agttcaacat attccatgaa agaagtacct ctttcgtcgc atgtttattt tatgtcatat
     5701 cctgatccca cagattcatg catgaaactt cggactcgca gctccacact tttcaaactt
     5761 cacgcctgcc ggataccgca ctgatgccaa aaaacaaccc cgaactggtc ggggtctcgg
     5821 tcatttcttg ttttcaaggt agaaccgcac tatctcttca agcagttcgt ccctttcaag
     5881 ccttcgtata acgctccttg cattcagatg attggtgatg tatgtaggat ctcccgaaag
     5941 gatgtagcca acgagctgat tgatcgggtt atagcctttt tcgttcaggg cctggtagac
     6001 cgtgaacagt atgtcccgcg cctcattctc cttatccttg tccattttga acatcattgt
     6061 ttcgcttccc gccatcatca acactcccct gccatcatgc gatgcccggc atgcgcccgg
     6121 ccttacgcaa aacacacgtc tgacgtgaat actattatat tataacatac ggaaattata
     6181 tgcaagtcgt ctgtccgcaa aacatcagat ttcgcccaga atgaaatcat tctcggcttc
     6241 cagcagcctc gtcggcagaa tatttcccga aatacccgga tctccctttg agagcaccct
     6301 tatatagttg ggcgtgaggc cttcatacca gcctttcatg tcattgtctt cctgttcata
     6361 cagcacgggc attacccgtc cgaggaagtt cctgttgaat tcaagctggc accggttcga
     6421 cagttcgatc agccggtcgc tcctctcttc cttgaccctg ccgtccacct ggtccttatg
     6481 ccgtgccgcc ggggtcccct tccttggaga atacttgaat acgtgcatct ttgcaaagcg
     6541 gatcttctcc aggaacatgt aagtcgtttc aaactcctcg tccgtctccc cagggaaacc
     6601 caccatcaca tcggtcgtga ccgatacatc cggtatttcc tccctcagca gttccactgc
     6661 cttcgcatat tcagccgtgg tgtatttcct gttcatcctt ttcagcgtct cgtcacagcc
     6721 gctctgcagc gatatgtggt aatgggggca gagcttttcc agcccggccg cctcggcgac
     6781 aaactctttg gtgatcatcg taggttccag cgatcccagc cttatcctct cgataccgtc
     6841 cacatcgtgg accatcctga tgaggtcggg cagcctgatc tgtcccaggt ctatgccgta
     6901 ggaagctata tgtatgcctg tcagcacgac ttccctgaac cccgcttcca ccagcttgtg
     6961 cacctcgctg atgacttctt cggccggccg gctgcgaata ggccctctcg catatggtat
     7021 tatgcagtac gagcaaaact ggttgcatcc ctcctgcact t
//
LOCUS       DAOSCC010000125         7058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6848_length_7058_cov_4.249036, whole genome shotgun sequence.
ACCESSION   DAOSCC010000125 DAOSCC010000000
VERSION     DAOSCC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6848_length_7058_cov_4.249036"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLG72_07525"
     CDS             <1..598
                     /locus_tag="PLG72_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ80684.1"
                     /translation="ISALLLKKKPDRAAFAGVIVALAGIFFMSGGLKFDFNIGDLLTF
                     ICAICWAFQIVFIDRFTQYEDAPLLAILQLLFTGLASSVLWLVVDIGKPMEFNATVAV
                     ILLITAVFGSSLAYGVQTIAQQYTTPVHTSLIFTAEPVFALVFALIIPNADGVRESLT
                     LMKIAGCLLILAGMIVSELRVGRGKDSTECDGRISNGV"
     gene            773..2779
                     /locus_tag="PLG72_07530"
     CDS             773..2779
                     /locus_tag="PLG72_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ80685.1"
                     /translation="MLSFPEVCRILSISAATGRNWIRLGKLKPADASAKRPVFARSEI
                     ERLVAAVEADGKVLKSRRNKKKVSGASLYENYITNSRNISVVNEILGQDRVHIDDDIM
                     KIIIANFALQFICRLNGKGRAERAGKAGNLIHDYLSNNLDVSGFGPLIDDLLHGVEDG
                     MGLVDECGEILEMELEYVPEEDSLGFAYISLKSMSQRKLSGAYYTPLSVVKELVGLAA
                     ETGGLAGRRILDPCCGTGNFLLYLASQSVGPECLYGQDSDELAVRIARINFALRFNIT
                     DMDFLKEHITCGDTLKTVPLSGYDLIIGNPPWGYDFTEEELAELAGVYRTASHKTAES
                     FDLFVERCLSLLSEGGLLAFVLPEAILNVRSHRAIRSILLEKAEFRFVSYLGEVFSKV
                     QCPSVILGVSNTKGMADRTDSGGIRVSAGGQTFTVSAERKFTPDTFNFHVSDEKQSCL
                     DEISRNAEMAYLKGNARFALGIVTGNNQAFISDRPLDGYEPVLRGSDIYKFRIAESHA
                     YMRFVPENFQQVAPAGMYRAPEKLMYRFICDTLVFAYDDRRTLSLNSCNILIPEIPGL
                     SVKYVLAVLNSSVAAFFCQQNFNSVKVLRSHLEAIPIPAASAGVQREITAMVDRIMEP
                     GAMVRKLYDELDGCIMRLYGLSAKNCGIIRESLKGKNRFLPPEP"
     gene            complement(2991..3536)
                     /locus_tag="PLG72_07535"
     CDS             complement(2991..3536)
                     /locus_tag="PLG72_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80686.1"
                     /translation="MDAELKEFRDGIFALRTRRFGTVAELMIEALFRFRRSRNQFHDR
                     YDAVEGKRIEIKFSTVMKENDARISRENLIEQCRNANVGNRALNSADIYRYPFDCNIQ
                     QVKRAEFDVLYYGLFFADRIAIFRMTCDDVLSCPGYSDFQHKGNEGEGQFHLNQKTIG
                     WHMENKFVQWLTYEELYNIFS"
     gene            3733..4866
                     /locus_tag="PLG72_07540"
     CDS             3733..4866
                     /locus_tag="PLG72_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOJ80687.1"
                     /translation="MPFKIVRNDITKMEVDAIVNAANPELKMGGGVCGAIFAAAGPEE
                     LQRECDRIGGCEVGQAVITGAGRLSAKYIIHTVGPIWRGGGNGEAKLLRGSYMNSLEL
                     AAEHKCKSIAFPLISSGIYGYPKDQALHIAVSAIGEFLMKNDMDVYLVVFDRSSYKLS
                     EKLFSAIGKYIDDNYVDARLDAERSRRLTRIEEQMMRGSDGYPAASGPADQASFGPVD
                     QAARRLADQAEYGPAKEEEIHLLRAAARPVSPRQKQKRRLEDIVGQLDETFSEMLLRL
                     IDEKGMTDVETYKRANIDRRLFSKIRSNKEYKPSKPTAIALAIALRLNLDETLDLLAK
                     AGYTLSMSSKFDVIIRYFIEEGNYNIFEINEALFAFEQDLLGA"
     gene            5093..5758
                     /locus_tag="PLG72_07545"
     CDS             5093..5758
                     /locus_tag="PLG72_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ80688.1"
                     /translation="MKRNFTELVFILDRSGSMAGLESDTIGGFNAMLKRQKNEPGDAV
                     VTTVLFDDRFELLHDRIDIKAVRPITEKEYYVRGSTALLDAMGHTISKIANVQKQTAE
                     EHRADRVMFVITTDGMENASREYTYEKIRDMVRRQKEKYGWEFIFLGANIDAVSTAES
                     VGITADRAANFVNDSEGISLNYKAVSEAVSAFRASRPIHGDWKKDIDSDYIKRGGRSK
                     KLH"
     gene            complement(6065..>7058)
                     /locus_tag="PLG72_07550"
     CDS             complement(6065..>7058)
                     /locus_tag="PLG72_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOJ80689.1"
                     /translation="EDVRSKFGKSAIGVIFPILSRNRFAVCLRGIAKGAGKIVLMLSY
                     PADEVGNHLVDIDSLDEKGINPYTDVLTLKQFRELFGNRKHTFTGIDYIDYYKSLAEE
                     FGIECEIIFSNNPRTILEYTKNVLACDIHTRARTKRILRSAGAETVYGLDEILTAPVN
                     GSGYNETYGLLGSNKATDDKVKLFPRNCSRLAENIQARLKELTGKTVEVLVYGDGAFK
                     DPVGKIWELADPVVSPGYTPGLEGTPNEVKLKYLADNEFRNLRGEEQKKAISEYIRNK
                     KKDLEGEMVSQGTTPRRLTDLIGSLCDLTSGSGDKGTPVIYIQGYFDSYAVQDR"
BASE COUNT         1703 a         1720 c         1952 g         1683 t
ORIGIN      
        1 catttcagcc ctccttctga aaaagaaacc ggacagggca gcctttgcag gcgttattgt
       61 cgcgcttgcc gggatcttct tcatgtccgg cgggctgaag ttcgatttta acataggcga
      121 tctgctgaca ttcatatgcg ccatatgctg ggcattccag atcgtgttca ttgacaggtt
      181 cacacagtat gaagacgcgc cgctgctcgc tatcctgcag ttgctgttta ccggcctggc
      241 aagctcggta ctgtggcttg tggtcgatat cggaaagccg atggagttca atgcgacagt
      301 ggctgtcata ctcctgataa cggcagtgtt tggctcttcg ctggcatatg gcgtgcagac
      361 cattgcccag cagtatacga cgcctgtcca tacttcgctg atattcaccg ccgaacctgt
      421 atttgcgctg gtgtttgcgc tgatcatacc caacgcggac ggcgtgaggg agtcgctgac
      481 actgatgaag attgccgggt gccttttgat acttgccggg atgatcgtat cggaactgag
      541 ggtcggaagg ggcaaggaca gtacggaatg tgacggccga atcagcaatg gagtttgatg
      601 gaagaagtgg aatacggaag gtcattcata tcgataccag cggagtggat acatgaaaaa
      661 catgtcgaga gcaataagcg gacagctttc attttttagt gattcggatg cagatcaaaa
      721 cggcttttca gattcaaaac atggtggcct ttctttcggt atgggggagg aaatgctttc
      781 ctttccagag gtttgcagga tcctttcgat atccgcggcc acggggagga actggataag
      841 gctcggcaag ctcaagcccg ccgacgcgtc tgccaaaagg cctgtttttg cccgttccga
      901 gatagagagg cttgtcgccg ccgtggaggc ggacggcaag gttttgaaat ccaggaggaa
      961 caaaaagaag gtttcgggcg catcgctgta tgaaaattac atcacgaaca gccgcaatat
     1021 ttcagtggtc aatgagatac tcggacagga ccgtgtacat atagacgatg atatcatgaa
     1081 aataatcatc gccaatttcg cgctgcagtt catctgccgg ctgaacggca aaggccgcgc
     1141 ggaaagagcc gggaaggcgg gaaacctgat tcacgattac ttgagcaata acttggacgt
     1201 ttccggattt gggccgctga tcgatgatct gctccatggt gtggaagacg gcatgggcct
     1261 tgtggatgaa tgcggtgaaa tactggaaat ggagcttgag tacgtcccgg aagaggacag
     1321 ccttggcttc gcctatatct cgctgaaaag catgtcgcag aggaagctca gcggggcata
     1381 ctatactccg ctgtccgtcg tgaaggaact tgtcggcctg gccgcagaaa ccggcggtct
     1441 ggcaggccgc aggatcctcg acccgtgctg cggcacgggc aacttcctgc tctaccttgc
     1501 gtcgcagtca gtggggccgg agtgccttta cggccaggat tccgatgagc ttgccgtccg
     1561 catcgccagg atcaactttg cattaaggtt caacataaca gacatggatt ttctgaagga
     1621 gcatataacc tgcggcgaca ccctgaaaac ggtgccgtta tccggctatg acctgataat
     1681 tggcaatccg ccctggggat atgattttac tgaggaagag ctggctgaac ttgcaggcgt
     1741 ttaccgcacc gcttcacata aaaccgcgga atccttcgat cttttcgttg agcgatgcct
     1801 ttcccttctg agcgaaggag gcctcttggc gttcgttctt cctgaagcga tcctgaacgt
     1861 gagatcccac agggccatac ggagtatcct gcttgaaaag gctgagttca ggttcgtttc
     1921 gtacctgggg gaagttttca gcaaggtcca gtgcccttcg gtcatccttg gcgtgtcgaa
     1981 cacaaaagga atggccgata ggaccgactc cggcgggata agggtaagcg cgggcggaca
     2041 gacttttacc gtcagcgcgg aacggaagtt cacaccggat acgttcaatt tccatgtgag
     2101 cgacgagaag cagtcttgcc tggatgaaat cagcaggaat gctgaaatgg catatctgaa
     2161 aggcaatgcc cggtttgcgc tggggatcgt tacagggaac aaccaggctt tcatttcgga
     2221 caggccgctg gacggctatg aaccggtgct gcggggcagc gatatataca aattcaggat
     2281 tgctgaaagt catgcatata tgagattcgt tcccgaaaac tttcagcagg tggcgcctgc
     2341 cggtatgtac cgtgctccgg aaaaactcat gtacaggttc atctgcgata cactggtttt
     2401 tgcatatgac gacaggcgga cactgtcgct gaacagctgc aacatcctca tcccggaaat
     2461 accaggtctg tcggtcaaat atgttctggc tgttctgaac tcaagcgttg cggcattttt
     2521 ctgccagcag aatttcaact ctgtgaaggt tttgcggtcc catcttgagg ctatacccat
     2581 tcctgccgct tccgccggag tacagcggga aatcacggcc atggttgaca ggatcatgga
     2641 gccaggcgcg atggtcagaa agctgtatga tgaactggat ggatgcatca tgaggcttta
     2701 cggactctca gcaaagaact gcggcataat cagggaatca ctgaaaggga aaaaccggtt
     2761 cctgccgccg gaaccgtaat acgtctgcca aatccactgg aacaggccat ccaactgtat
     2821 agtgcaccaa aacaggttat caggctgcat gatccttcat ggaatgccgt catccgactg
     2881 tatggttggt catccggttg catggttcgc tggaacaagc tgtccggttg tttgttccac
     2941 tattgagcga actctatggg ccatcgttaa gtgtcgtccg tccgcatggc tcacgaaaaa
     3001 atgttataca gttcttcgta tgtcagccac tgcacgaatt tgttttccat gtgccagccg
     3061 atggttttct ggttgaggtg gaactgtccc tcgccttcgt tgcccttatg ctggaaatcc
     3121 gagtagcccg ggcaggagag gacatcgtcg catgtcatcc tgaaaattgc gatcctgtct
     3181 gcaaaaaaca gcccatagta caggacgtcg aactcagcac gtttgacctg ctggatgttg
     3241 caatcgaaag ggtatctgta tatatcagcg ctgttgagcg cgcggttgcc gacattggca
     3301 ttcctgcact gctcgatcag gttttctctt gagatccggg catcgttctc cttcatgacg
     3361 gtcgagaact tgatctctat acgcttgcct tcaactgcgt cataacggtc atggaactgg
     3421 ttcctggacc tgcggaaccg gaacagcgct tctatcatca actccgcaac agtgccgaac
     3481 ctgcgcgtgc gcagagcgaa tatcccgtcc ctgaattcct ttaattctgc gtccattgct
     3541 gttctccttt gctatgtccg ttggtgcgtt taaaatgtgt tatggacagt ttaccataac
     3601 aggcaggacg acgcaatata ctgctgaata tgtcggaata ggatctgccg caggcaggac
     3661 tctttacggc aaagtattat agtcgtcaaa atgttataat attaccagaa cttgtccgtg
     3721 ggggttgttt gtatgccgtt taaaatagtg cgcaatgata taacgaaaat ggaagtcgac
     3781 gccatcgtga acgcagcgaa tcctgaactg aagatgggcg gcggtgtatg cggggcgatt
     3841 tttgccgctg ccggaccgga agaactgcag cgggaatgcg accggatcgg cggatgcgaa
     3901 gtggggcagg ctgttataac cggcgccggc aggctttccg ctaagtacat aatccatacc
     3961 gtggggccga tatggcgggg cggcggcaat ggcgaggcga agcttctgcg cggcagctac
     4021 atgaattccc ttgaactggc tgcagaacat aaatgcaaat ccatcgcgtt tccgctcata
     4081 tcatcgggga tctacggtta tccgaaggac caggcgctgc acatagcagt ttccgccata
     4141 ggcgagttcc tgatgaaaaa tgatatggac gtctatcttg tggtgtttga caggagctca
     4201 tataaactga gtgaaaagct attttcagcg atcgggaaat atatagacga taattacgtc
     4261 gatgcgcgcc tggatgctga acgatccagg agacttaccc gtattgaaga acagatgatg
     4321 aggggatccg acggttatcc ggccgccagc gggcctgccg atcaggcttc attcgggcct
     4381 gtagaccagg ctgcacgaag gttggccgac caggccgaat acgggccggc caaagaggaa
     4441 gagatccatt tgctcagagc cgcagcacgg ccggtttctc ccaggcagaa acagaaacgc
     4501 aggctggagg acattgtcgg ccaactggat gaaacgtttt cggagatgct gctgaggctg
     4561 atcgatgaaa aaggcatgac cgacgtggag acatacaaga gggcaaatat cgaccgcagg
     4621 ctgttttcaa aaatacggag caacaaggag tacaaaccaa gcaaaccgac agcgatagcg
     4681 ctggcgatcg cccttaggct caaccttgac gagaccctcg atctgcttgc caaagcaggg
     4741 tatacgcttt ccatgagcag caagtttgac gtcatcataa ggtatttcat agaggaaggc
     4801 aactacaata tatttgagat aaacgaggct ctcttcgcgt tcgaacagga tctgctcggc
     4861 gcgtgaacaa gcagcccatg gctctgccgt cacagggcct gaacggctgc acggggagta
     4921 aaccggccgg tccgtggtgc ggctgtccgc ccgttttgaa atttgtcgct tcgaaggcga
     4981 ccataatatt cctgcgcggt gttatccttt ggtcatacag gaaagtcaat tccaggctga
     5041 aggctttcaa ggccggtatg attcctggat cagaatggag gagggtaaca ccatgaaaag
     5101 aaacttcacc gaactggtat tcatccttga caggagcggc tcgatggcag ggctggaaag
     5161 cgacaccata ggcggcttca acgcgatgct gaaaaggcag aagaatgagc cgggagatgc
     5221 cgtggtcacc accgttttgt tcgacgacag gttcgaactg ctgcatgaca ggatagatat
     5281 aaaggctgta aggcctatca ccgaaaagga gtattacgtc cgcggcagca cggcccttct
     5341 ggatgccatg ggtcatacca tcagtaaaat agccaatgtg cagaagcaaa cggcagaaga
     5401 gcacagggcg gacagggtga tgttcgtcat taccacggac ggtatggaaa acgccagccg
     5461 tgagtatacc tatgaaaaaa taagggatat ggtcaggcgt caaaaggaga agtacggctg
     5521 ggagttcatt ttcctcggtg ccaacatcga cgcggtttcg actgccgaaa gtgtcggaat
     5581 aaccgcggac agggcggcaa acttcgtaaa tgacagcgag gggatcagcc tgaattacaa
     5641 ggccgtcagc gaagcggtaa gcgcattccg tgcctccaga ccgatccatg gggactggaa
     5701 aaaagatatc gacagcgatt atatcaaaag gggaggcaga agtaaaaagc tgcattaaga
     5761 aaagctgtaa ctgcatcaaa caattgagac tgagatcaca ttaaaagaga tgcctgcaga
     5821 acagggtcca gtccctgcgc tgcaggcagt tttactgata gatatcatat atatgtctta
     5881 aaggatcttg aatgatactg cccatattga cgattataga catcgcatat attgacagcg
     5941 gatattgcag caaatcgtca caggtatcca atatatcgat atttatgtgc atactttact
     6001 gcgtattcta tacggatatc ttaagtgcct gattcatgcg tacgcatgga atctgatccc
     6061 gggatcaacg atcctgcact gcgtagctgt cgaagtatcc ctggatgtat atcaccggcg
     6121 ttcccttgtc gccgctgccg ctggtcaggt cgcacagcga acctatcagg tcggtcagcc
     6181 ttcttggtgt cgtgccctgg gataccatct ctccctcgag atcctttttc ttgttcctga
     6241 tgtattcgga gatcgccttt ttctgttctt ctcccctcag gttcctgaac tcgttgtcgg
     6301 caaggtactt cagcttcacc tcgtttggag tgccttcaag gcctggagta tagccgggcg
     6361 atacaacggg atcggccagt tcccatatct tgcccacagg gtccttgaac gcgccgtccc
     6421 cgtaaaccag gacttcgacg gttttgcctg tgagttcctt caggcgggcc tgtatatttt
     6481 cagcaagccg gctgcaattg cgcgggaaaa gtttgacctt gtcatccgtg gccttgtttg
     6541 agccaagcag gccgtaagtc tcgttgtatc cgctgccgtt gaccggtgct gtcagtattt
     6601 cgtccaggcc gtacactgtt tccgcgccgg cggatctcag gatccttttg gtacgcgccc
     6661 tggtatggat atcgcatgcc agcacatttt tcgtatattc gaggatcgtc ctcgggttgt
     6721 tcgagaatat gatctcgcac tcgatcccga actcttccgc cagcgattta taataatcga
     6781 tatagtcgat gcctgtgaaa gtgtgcttcc tgttgccgaa cagttcacgg aactgtttca
     6841 gtgtgagcac atcggtataa ggattgatgc ctttttcgtc aagcgagtcg atgtccacga
     6901 ggtggttgcc gacttcgtcg gccggatagc tgagcatcag cactatcttc ccagcgccct
     6961 tggctatgcc gcgaaggcag accgcgaagc ggttcctgct gaggatcggg aaaataactc
     7021 cgatggccga ctttccgaat ttcgagcgga catcctcg
//
LOCUS       DAOSCC010000126         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6851_length_7055_cov_4.276286, whole genome shotgun sequence.
ACCESSION   DAOSCC010000126 DAOSCC010000000
VERSION     DAOSCC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_7055_cov_4.276286"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>224
                     /locus_tag="PLG72_07555"
     CDS             28..>224
                     /locus_tag="PLG72_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80690.1"
                     /translation="MKLRIHAFFVLLIITKACIYYNLVGIEKYPLATVLLTAVYMAVI
                     YFICGSKKLAYCFISLLISVL"
     assembly_gap    225..234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <235..2413
                     /locus_tag="PLG72_07560"
     CDS             <235..2413
                     /locus_tag="PLG72_07560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOJ80691.1"
                     /translation="ANVLYNRYFHMFFSLNIILQAGKLKEVGGVAFDLLKPADLLLFI
                     DPVLPVILIAASNKHRTGQKRGAGWTGPVSRAAMFLAMAGSFAFFAVNPTDSKLVTAV
                     NHQEIFTWYFRDSLVRSETDTADAADTEKMEIGTATAEQLSGEGYLNGIAAGRNLIVI
                     QMESFQNFLINAVYNGREITPNLNKLAAGESIYFSSYYQQLGRGNTSDAEFVTNNSLY
                     PVIFGPAYELYQGNRFYGLPWVLKENGYRTVALHGYKKSFWNRDKAFPGQGFDMFIGE
                     EDLAVDEKIGFGLSDKSFFRQAAEHLKNMEQPFYSFIITLSSHVPYKMPASCQELELL
                     PGDEDTLFGNYIQAVHYTDEAIGEFINYLKELGLYDNSIIALYGDHFGIDCKNEAAEQ
                     VKAFLGRDYTYNEMLNIPLIIHIPGSGIHETVETTGGQVDFMPTVLNLLGISGENLVM
                     FGHDLLQAGSGFVASQTYMLKGSFIDDEKVFEMARTGIFEDSKAWDRKTGEPVSIDTC
                     REGYERAIKEIAKCTYLLENDLLRGMDLGGGTTAARGGNLTDIGQSVNEILTSHGTVT
                     DIGTMDRIGEDPGILDTMYGEGFKLYFAELQAKEPGTLALPDGHSFDKLASWLSGHSD
                     SFLVVPVTVGDISVLENLKKLYPDICRQIIPQISDLGDYVKVEYQGFRRIILDLRSSG
                     IDADKLAVFLDRNRVSGAVLSEGQLSDAQLLEVLESRDVAVLK"
     gene            2558..3646
                     /gene="ychF"
                     /locus_tag="PLG72_07565"
     CDS             2558..3646
                     /gene="ychF"
                     /locus_tag="PLG72_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ80692.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITKAGAEIANYPFCTIEPNVGIVPV
                     PDERLDKLAEMYNPAKVTPATVEFVDIAGLVKGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFDDSNIVHVDGSVSPSRDAETINLELIFADLEMLERRIDKAKKMLKGDRKYQAEVE
                     LYERIKETLESGMPARAMDFTEEEQHLVDQLFLLTSKPVLYAANISENDLQNSENPYV
                     KELSEIARREGAEIMVICAKLEEEIAQLEDEERKLFLEELGLKESGLDKLIKASYRLL
                     GLMSFLTAGPTEVRAWTIRKGTKAPQAAGKIHSDMERGFIRAEIVSYDDLVRYGSYAA
                     ARDNGLVRSEGKDYVMQEGDVVVFRFNV"
     gene            complement(3672..4133)
                     /locus_tag="PLG72_07570"
     CDS             complement(3672..4133)
                     /locus_tag="PLG72_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ80693.1"
                     /translation="MSELNVVIFNLNGQLFGADASQVFQIIRYQEPVRMPKMPSFIEG
                     VINYRDTVLPVINLVKRFELGNMDVNKKTKILVARIGEKYAGFLVNDVTEIIRFAENE
                     VEEAPSVVNAGTASYLKKIGKKEDMLYSIIDLENILNEAEKKRLPAPQPKA"
     gene            4661..5722
                     /gene="aroF"
                     /locus_tag="PLG72_07575"
     CDS             4661..5722
                     /gene="aroF"
                     /locus_tag="PLG72_07575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ80694.1"
                     /translation="MIVVMSRNATKQEIDNVENKLRELGFKTHPIVGEVKTVIGAIGD
                     KRILNTVNITTMPGVESIVPIMKPFKLAGRELKQTPSIIDVGGIKFGGKELAVIAGPC
                     AIESEESFIETALKVKEAGACILRGGAFKPRSSPYSFQGLQEDGLRIMAKAREITGMK
                     IVTEVVDTRDVELVASYADMIQIGARNMQNFRLLHEVGLTSKPILLKRGLAATIEEWL
                     MAAEYIMNAGNENIILCERGIRTYETSTRNTIDISAVPVVKELSHLPIIADPSHAAGN
                     WKYVAALSRAAVAAGADGLIVEVHIDPPNALSDGPQSLRPIKFKQLMEELELVAAAVG
                     RTIRKSGTDDVPEKTGRPN"
     gene            complement(5759..6880)
                     /locus_tag="PLG72_07580"
     CDS             complement(5759..6880)
                     /locus_tag="PLG72_07580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ80695.1"
                     /translation="MVDERPNRINTRKEAKSIRYGNQGVIIILVYRWLVFLFTAILSI
                     TGSSFDTQGYPILESLAVIAVYNGAVTAYCLNNGKKYAGSLLYIDIVAISLLILFTGG
                     INSDLYIFYFLPLCLYGIKNDLPGTVRITGFTAVFYSGFCFLSSMLFNTRLNWFRLII
                     YDFLFLLAAFAVTRLSFEVKKYDELRKKEFKLARTDKLTGLANRHYFDQKIREEAAYA
                     DAWNSVINILMFDLDNFKGYNDSYGHASGDKLLKLFADIIKQCIRKTDIPVRYGGEEF
                     LIMIRDMDVIIAKSVGERIRKQLEKQCIRLGSKFGRQVVTASCGIAQYPVHAPNIKDV
                     IEMADQALYHAKAIGKNIVACYDEIGMTREELERKQELD"
BASE COUNT         1891 a         1685 c         1785 g         1684 t
ORIGIN      
        1 gttgtaatat atagtacagg tgattttatg aagctcagga tacatgcttt ttttgtgctt
       61 ttgatcataa cgaaggcctg catatattat aacctggtag gtattgagaa gtatccgctc
      121 gccactgtgc tgctgacagc cgtatatatg gctgtcatat atttcatatg cggatcgaaa
      181 aaactggcat attgttttat cagcctgctg atctctgtac ttatnnnnnn nnnnggcaaa
      241 tgtactgtat aacagatatt tccatatgtt cttctcattg aacattatcc tgcaagcagg
      301 caagctaaag gaagtcggag gagtcgcctt tgacctgctg aagccggcgg acctgctttt
      361 gtttatcgac ccggtcctgc ctgtcatact catcgccgcg tcaaataaac acaggacagg
      421 gcagaaacgc ggagcaggat ggacaggccc tgtatcaagg gcggcaatgt ttctcgcgat
      481 ggcgggatcc tttgcgttct ttgccgtgaa tccgacggac agcaagcttg tcacagcagt
      541 caatcaccag gagatattca cctggtattt cagggacagc ctcgtcaggt ctgaaactga
      601 tacagccgat gcagccgata cggaaaaaat ggaaattggg acagctactg ccgaacagtt
      661 gagcggcgaa ggttacctga acgggattgc ggccggaagg aatctcatcg ttatccagat
      721 ggagtcattt cagaatttcc tgataaatgc agtgtacaac ggccgtgaaa tcactccgaa
      781 cctcaataag ctggcagccg gtgaatctat ctatttcagc agttattacc agcagctcgg
      841 cagaggcaat acatcagacg cggagttcgt gacaaacaat tcgctttacc ctgtcatatt
      901 cggcccggca tatgaactgt accagggcaa ccgcttctac ggcctgccgt gggtcctcaa
      961 ggagaacggc tatcggacgg tcgccctcca tggttataaa aagtctttct ggaacaggga
     1021 taaggctttt cccgggcagg gtttcgacat gttcatcggc gaggaagatc tcgcggtcga
     1081 tgaaaaaatc ggcttcggcc tgtcggacaa gtccttcttc aggcaggcgg cagagcattt
     1141 aaaaaatatg gagcagccgt tttacagctt catcataacc ctgtcttcac atgtccccta
     1201 caaaatgcct gcctcctgcc aggaactcga actgctgccg ggagacgaag acacgctttt
     1261 cggaaactac atccaggcag tccattatac tgatgaagca ataggcgagt tcattaacta
     1321 cctgaaggaa ctgggcctgt acgacaattc gatcatcgcg ctgtacggcg accatttcgg
     1381 catagactgc aaaaatgagg ccgccgaaca ggtaaaggcc ttcctcgggc gagactatac
     1441 ctacaacgag atgcttaaca taccattgat aatacacatc cccggctcag gcatacatga
     1501 aacggtcgaa acaacgggag gacaggtgga tttcatgccg acagtgctta accttttggg
     1561 gatctccgga gagaacctgg taatgttcgg ccatgacctg cttcaggccg gatcaggctt
     1621 tgtcgcatcc cagacctaca tgctcaaggg ttctttcatt gatgacgaaa aagtttttga
     1681 gatggcaagg acaggcatat tcgaagacag caaagcatgg gacagaaaaa cgggagagcc
     1741 tgtaagcatc gacacatgcc gtgaaggata tgaaagagcc ataaaggaaa tagcaaaatg
     1801 cacatatctg ctggaaaacg acctgctcag aggcatggac ctgggggggg gaaccacagc
     1861 cgccaggggc ggaaatctca ctgatatcgg ccagtccgtc aatgagatcc tcacgagcca
     1921 cggcactgta actgacatag gcacgatgga caggatcggt gaagatccag gtattcttga
     1981 tacgatgtac ggcgaaggct ttaaactgta cttcgcagaa ttacaagcca aagagcccgg
     2041 aacgctcgcg ctgccggacg ggcacagttt tgacaaactt gcgtcatggc tctccgggca
     2101 ttcggactca ttcctggtcg tccctgttac cgtcggcgat atcagtgtcc tggagaacct
     2161 gaaaaagctg tatccggaca tatgccggca aataatcccg cagataagcg atttgggcga
     2221 ctatgtaaaa gtggaatacc agggattcag aaggatcata cttgatctga gatcatccgg
     2281 catagatgcg gacaagctgg ctgtattcct ggacaggaac agggtttcgg gggcagtact
     2341 gtcagaagga cagctttcag acgcgcaact gcttgaagta cttgagtcga gagatgttgc
     2401 cgtgctaaaa taaggtgccg gagaggttgc cttgcgtcct gtttttgttg acagtttcga
     2461 caataaagat ataatttgtt ttgaccggtc cggagataag tgccggcagt gctgccggct
     2521 ataaggcgat cagaaaaaga aagaaggatg gacatatatg aagcttggta tagtaggcct
     2581 gcccaatgta ggcaaaagca cacttttcaa cgcaataacg aaggccggcg cagaaatagc
     2641 caattatccc ttctgcacca ttgaaccgaa tgtgggtatc gtcccggtgc ctgacgaaag
     2701 actggacaaa cttgccgaaa tgtacaatcc tgccaaagtg acaccggcaa cagttgaatt
     2761 tgtggatata gcgggccttg tcaaaggggc cagcaaaggt gaaggccttg ggaacaagtt
     2821 tctctcgcat atccgggagg tcgatgcgat agtgcatgtg gtccgttgct tcgatgacag
     2881 caacatagtc catgttgacg gttccgtatc tccttccagg gatgctgaga cgataaacct
     2941 cgagcttatt ttcgcggacc tggaaatgct ggaaagacgc atcgacaagg cgaaaaagat
     3001 gctgaaaggc gacaggaaat accaggcgga agtcgaactg tacgaaagga taaaagaaac
     3061 cctggaatcc gggatgccgg caagggcaat ggatttcacc gaagaagagc aacacctggt
     3121 ggaccagctt ttcctgctca cttccaaacc tgtcctgtat gcagccaata tctcggagaa
     3181 tgatctgcaa aacagtgaga atccttatgt taaagaactc agtgagatcg cccgcaggga
     3241 aggcgcggag ataatggtga tctgcgcgaa actcgaagaa gaaatcgccc agctggaaga
     3301 tgaagaaagg aagctgttcc tcgaagaact cggactgaag gagtcaggtc tcgacaagct
     3361 gataaaagca agctacaggc ttcttggcct tatgagtttt ctgaccgcag gcccgacgga
     3421 agtaagggca tggaccataa gaaaaggcac caaagcgccg caggccgccg gcaagataca
     3481 cagtgacatg gagagaggtt tcataagggc cgagatcgta tcatacgacg acctggtcag
     3541 gtacggttca tatgctgcgg cacgcgacaa cggacttgtc cgttccgaag gaaaggatta
     3601 tgtcatgcag gagggagacg tggtcgtttt caggttcaat gtttaactat catgcttctg
     3661 atattttgct atcaggcttt tggttgcgga gcagggagtc tttttttctc tgcttcgttt
     3721 aatatgtttt caagatcgat tatggagtac aacatatcct cttttttgcc gatcttctta
     3781 aggtatgacg cggtcccggc atttacgaca gacggggctt cttccacctc attttccgca
     3841 aacctgatga tctctgtcac gtcgttgacc aggaatcctg catacttctc gcctatcctg
     3901 gcaacaagga tctttgtctt tttattgaca tccatgttcc ccagttcaaa cctcttcaca
     3961 aggtttataa cgggaagtac ggtgtccctg taattgatga ccccttcgat gaaagaaggc
     4021 atcttcggca tcctgacagg ctcctggtat cttattatct ggaatacctg cgatgcgtcg
     4081 gccccgaaca actggccgtt aaggttgaat attacgacat tcagttcact catatgacag
     4141 cctccctggt atttggcccc tggccggtcc tgcgccggaa aacagttgcc agttatatta
     4201 tagcacagaa tttcgacaca ttgtaaccaa aaaactctct tgacagtaaa aacacaccat
     4261 gctaagatta tataaattta ttcactgcaa aaacatgcaa ttaaactgac aaaggcaatg
     4321 accaagggaa agtaggccgc acgatgcctg tataccagga agcgttacgg aattccgcaa
     4381 tcaggttcgt tccgtaacag ttccgtgcag cgagccggga atggtgggag cccggcctgg
     4441 tatgacggtc gaagttcccc tttgagccgc aggctgaaag atacagtagg ccgagccggg
     4501 tttcccccgt tacagggata gcgcatgata gtgcgtgaca gagtgggcgt ttgccaattt
     4561 gagtggtacc gcgtggagta taactcttcg tctcatacga gatgaagagt tttttaatta
     4621 atgtgcaata tccgattcaa cttgaaggag gactgatgtc atgatcgtag taatgagcag
     4681 gaacgccacg aagcaggaaa tcgacaatgt tgaaaataag ctacgggaac ttggtttcaa
     4741 aacacatccg atcgtaggag aagtaaaaac cgttattggc gccatcggcg acaaacgtat
     4801 cctgaatact gtaaacatca ccacgatgcc cggagtcgaa agcatcgttc ctatcatgaa
     4861 gccgttcaaa ctggcaggca gagaactgaa acagactcca agcatcatcg atgtcggcgg
     4921 gataaagttc ggaggaaagg aactggctgt cattgccgga ccatgtgcca tagaaagcga
     4981 agagtctttt atagaaacgg ccctgaaagt gaaggaagca ggcgcgtgca tcctgagagg
     5041 aggagccttc aagccccgtt cttccccata ttctttccag gggcttcagg aagacgggct
     5101 caggatcatg gcaaaagcac gtgaaatcac gggcatgaag atcgttaccg aagtagtgga
     5161 cacccgggac gtcgaactgg tggcatccta cgcggacatg atccagatag gcgcaagaaa
     5221 catgcagaat ttcaggcttc ttcatgaagt gggcctcact tcgaaaccca tactgcttaa
     5281 gcgcggcctg gccgccacga tcgaagaatg gctcatggcg gcggaatata taatgaacgc
     5341 cggaaatgag aacatcattt tgtgtgaaag aggcatacga acttatgaaa cttcgacaag
     5401 aaacacgata gatatcagtg cggtaccggt agtaaaagaa ctctcccatc tgccgattat
     5461 tgcggatccg agccatgccg caggcaactg gaagtatgtc gccgccctgt ccagggccgc
     5521 tgtagctgca ggtgccgacg ggctcatcgt cgaagtccac atcgatccgc caaacgccct
     5581 gagtgacggg ccccagtccc tcaggccgat aaaattcaag cagctgatgg aagaattgga
     5641 actggtagcc gctgccgtcg ggcgtacaat cagaaaatcc gggacagacg atgtcccgga
     5701 aaaaaccggt aggccgaact gatataatgt cagggacctc cgtgtcccgg aggtcccatc
     5761 agtccaattc ctgtttcctt tcgagttctt ccctggtcat gcctatctca tcatagcagg
     5821 ccactatgtt cttgcctatc gccttggcat ggtaaagcgc ctggtcggcc atttctataa
     5881 catcctttat atttggagca tgcaccggat actgggcaat gccgcagctt gcagtcacaa
     5941 cctgccttcc gaacttgctt cccagccgga tgcactgttt ctcaagttgt ttcctgatcc
     6001 tttcgccaac gctcttggct atgatgacgt ccatatccct tatcattatc aggaactctt
     6061 ctcccccata tctcacgggt atatcggttt tcctgatgca ctgctttata atatctgcaa
     6121 agagtttcag cagcttgtcc cctgaagcat gaccgtaaga atcattataa cccttgaaat
     6181 tgtcaaggtc gaacatcagt atgtttatca cggaattcca tgcgtctgca tacgccgctt
     6241 cctccctgat cttctggtcg aaatagtggc ggttggcaag cccggtcagc ttgtcggtgc
     6301 gggcaagctt gaattccttc ttcctgagtt cgtcgtattt cttcacttca aagctcagcc
     6361 ttgttactgc gaacgcagcc aaaaggaaca ggaaatcata tattatcagt ctgaaccagt
     6421 tgagcctggt gttgaacagc attgaagaca ggaaacagaa ccctgaataa aatacggctg
     6481 taaaaccggt tatcctgaca gtgccgggca aatcattctt tatgccgtac aggcacagcg
     6541 gcagaaaata aaaaatatac aggtcggaat ttatcccgcc tgtgaaaaga ataagcagcg
     6601 aaatagcgac tatatcgata tagagaaggg atcctgcgta cttcttcccg ttgttgaggc
     6661 aatatgcagt cacagcgccg ttgtagaccg caatgacagc aagcgactca agtatcgggt
     6721 acccctgggt gtcaaagctg ctgcccgtaa tggacagtat agctgtaaac aggaatacga
     6781 gccaccggta aaccagtatt atgattactc cctggttccc atacctgatg ctcttggctt
     6841 cttttcgggt attgatccgg ttcggtcttt cgtccaccac ttctcacctg cgttttagat
     6901 gttggggatc aggtttatcg gggcgccggt cccttgaccc ggaattcagc ttcttcttca
     6961 acaagttctc cgtttttgta gtaaatcctg atacaggcgg cattttcact gtctccggct
     7021 tgctggtcta tcgggatata tatatctaca ttttt
//
LOCUS       DAOSCC010000127         7015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6911_length_7015_cov_4.920977, whole genome shotgun sequence.
ACCESSION   DAOSCC010000127 DAOSCC010000000
VERSION     DAOSCC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_7015_cov_4.920977"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PLG72_07585"
     CDS             <1..1297
                     /locus_tag="PLG72_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ80696.1"
                     /translation="AAYAIVSYQTAYMKAHYPVEFMAATLNSFVGNSDKISYYVQECR
                     QEGIEVLPPDVNESDVRFTVANGKIRFGMAAVKNVGESAVRELIDERDKRGLFKNFYD
                     FCERIGERDFNKRCVESLIKCGAFDSFGVYRSKMMAVYEKLLESAQQGKKSRVEGQLS
                     LFEMSGQIQKMKTEESYPDLKEFSREMLLSMEKEMLGLYISGHPLDEFKNELMEQITV
                     NSKDINAYGSEGEDDGSGGTGLIDGMLVTVGGIITEVKTKTTRSNNLMAFVTLEDLYG
                     SMEVIVFPTVLTRYRHQLTSDNVVIIDGRISIKEEEVPKIICENVRMLKKKEISENGH
                     VMGANGVSAKNERYEILYIRSDIQENSDMMKSVISLLKFFSGKVPVVFCRSDETGNIA
                     RANMGEYRVDACDTLQRELKERFGEDNVKLVSKAMQKQQ"
     gene            1400..1633
                     /gene="mtrB"
                     /locus_tag="PLG72_07590"
     CDS             1400..1633
                     /gene="mtrB"
                     /locus_tag="PLG72_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HOJ80697.1"
                     /translation="MDWELDKLIGEFIVVRAEENGVNVIGLTRGKDTKFHHTEKLDKG
                     EVLLAQFTENTSAIKVRGKSRILCRHGEIISGE"
     gene            1655..1852
                     /locus_tag="PLG72_07595"
     CDS             1655..1852
                     /locus_tag="PLG72_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80698.1"
                     /translation="MWTVVYMAQSKEIATALQELLTKEGILVKLRPISKNHDNSDDYY
                     EVLVPEAEVEEAHSVIIEKGY"
     gene            1945..2367
                     /locus_tag="PLG72_07600"
     CDS             1945..2367
                     /locus_tag="PLG72_07600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOJ80699.1"
                     /translation="MFVSETPLVVRYAETDQMGVVHHSNYPIWFEAGRTDFIRKVGMP
                     YSVIEQKGAMLPLLELRCRFMSFARYEDNIIIKTYIKEFTGTRLCFSYQAVREEDGRI
                     LAEGETLHCWTRRDLKPVNIKKYMPEIYELIEKAAKDN"
     gene            2446..2991
                     /locus_tag="PLG72_07605"
     CDS             2446..2991
                     /locus_tag="PLG72_07605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOJ80700.1"
                     /translation="MNIPNILTILRFLLIPAFAYFLSVRSYVVAVLLFLAGGLTDVLD
                     GYIARRYNLITSWGKIADPMADKLMQITAVVILSAVLKIIPIELVLVILAKEILMGLG
                     ALVLYRQKKYVVSANWYGKMTTVIFYLAIVLLIFDEPFGRWTLLGVAVNQLVFILAVA
                     ATLFAFFMYFRSYLRIKQQKQ"
     gene            3001..3888
                     /locus_tag="PLG72_07610"
     CDS             3001..3888
                     /locus_tag="PLG72_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOJ80701.1"
                     /translation="MFGYIVPEKPEMKIKEYELFRAYYCGVCRAIGRRCGQLKRLTLS
                     YDTTFLAVLLSAASGEKVQVMKKRCALHPLEKRHVISGSSAVDYASDINIILAYYNLE
                     DKKRDKDSPVSASALVLLKGAFRRLRRKYPEKCRIMEQRLDELVRLEKQQCPSMDMAA
                     EPFARLMEEITAYEPLCTGKDTEKILRWLGYNLGKWIYLLDAFDDLETDIKRGNYNPF
                     VCQFGYTGQDVDGFRNEIREKAGFTLFYTLSEIARAFELLEAKINRGILENIIYIGML
                     RKTENILGIRRCGEVEESV"
     gene            3872..4504
                     /locus_tag="PLG72_07615"
     CDS             3872..4504
                     /locus_tag="PLG72_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ80702.1"
                     /translation="MKNPYEVLGIREGASEEEIKKAYRELVKKYHPDQYRDNPLSKLA
                     EEKLAEINEAYDYLMKNVSSGGNSQYSGGASYGRSGADGWSDSGSDFFNQVKSHINSG
                     NIRLAEEMLDSSRSRPAEWYYLKGIIFIRKGWYNEAYTHIQRAVNMDPANYEYRSALD
                     RMNTSYNAYRTNPFNSGRYRSEPDLCTICQCLWCSDCCCECMGSDLISCC"
     gene            4523..5044
                     /locus_tag="PLG72_07620"
     CDS             4523..5044
                     /locus_tag="PLG72_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80703.1"
                     /translation="MRGPFKSKTIALNGVLGALAVICLLLADVLPTSKISLYVLSSFF
                     VSVAIIEGGIRAGLIFYAATALVGFIILPDKISMIPYVLFFGTYGIVKFYIEKLNCIV
                     IEYVLKVIFFNACAGLALLAAGSLFEGGLASGLPWGILAGICQVIFLVYDIVYTLFIN
                     YYRDRLRTRILKR"
     gene            5080..>7015
                     /locus_tag="PLG72_07625"
     CDS             5080..>7015
                     /locus_tag="PLG72_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ80704.1"
                     /translation="MEERTLRILEFVKIKEKLVSLCSSELGKELASVLLPQTQPEEVE
                     RMLKETSDAVDFILRRGSPPLGGIHDIRDTLRRVELGTALNPGELLRVADTLRAARNL
                     KNYASEADPRADESGNHIRNLIASVTPNKRIEDSINAAIISDEEISDNASSTLASIRK
                     RIREEQESLKDKLNSMIHSSKYQKFMQESIVTIREDRYVIPVKAEYRNEIPGLVHDSS
                     ASGATVYIEPMAVVEANNNIKQLRIKEQLEIERILQELTGEVAGIIEPLKANMTLFAQ
                     LDFVFAKAKLSLDLKCVCPKLNREKRINIKKGRHPLLDSKTVVPIDLWIGERFTTLVI
                     TGPNTGGKTVTLKTVGLFTLMVQSGLHVPAAEGTEMSVFDNVFADIGDEQSIEQSLST
                     FSSHMRNIVEILSRADDMSLVLFDELGAGTDPTEGAALAMSILENLHGRGAITIATTH
                     YSELKVYALTTDGVENACCEFDVETLRPTYRLLIGVPGKSNAFAISKRLGLSDDILDR
                     AREFLSGEEIQFEDVLMTIERNRRESEQERLQAESLRLEIEKLKKELEEQKYKLSSQR
                     EKLLREAREEARRILLDARKDAGDILEEMKQATKLQDEKERRRAAEEAKAKLRGSIGK
                     IEESLAEGLFRRKGFVKPPEN"
BASE COUNT         2066 a         1479 c         1955 g         1515 t
ORIGIN      
        1 cgcggcctac gccatcgtct cctaccagac cgcctatatg aaggcgcatt atccggtcga
       61 attcatggcg gcaacgctca acagttttgt cgggaacagc gacaaaatat catattatgt
      121 acaggaatgc aggcaggaag gaatcgaagt gctgccgccg gatgtcaatg aaagcgatgt
      181 gaggtttacg gtggcaaacg gtaaaataag attcggaatg gctgccgtaa agaatgtcgg
      241 ggaaagcgcg gtgcgggaac tgatagacga aagggacaaa agaggcctgt tcaaaaactt
      301 ctatgacttc tgcgagcgca taggagagag ggatttcaac aaaagatgcg tcgaaagcct
      361 gataaagtgc ggagcgttcg attcattcgg cgtatacaga tcgaaaatga tggctgtcta
      421 tgaaaagttg cttgaaagtg cccagcaagg caaaaagagc agggtggaag gccagctttc
      481 gttattcgag atgtccggac agatacagaa aatgaaaaca gaggaaagct atccggacct
      541 taaggagttt tccagggaga tgctgctttc gatggagaag gagatgcttg gcctgtacat
      601 ttcggggcat cctcttgatg agttcaagaa tgaactgatg gagcagataa ctgtcaacag
      661 caaggacatc aatgcatacg gaagcgaagg ggaggatgac ggttcaggcg gaacaggcct
      721 catcgatggg atgcttgtta cagtgggcgg cataatcacg gaagtgaaga cgaaaaccac
      781 caggagcaat aacctgatgg ctttcgtgac actggaagat ctctacggaa gtatggaggt
      841 gatcgtattt ccgacagtgc tgaccaggta caggcatcag ctgaccagcg acaatgtggt
      901 gattatagac ggacgcatca gcataaagga agaggaagtg cccaaaatca tatgtgaaaa
      961 cgtaaggatg ctgaaaaaga aagagatcag tgaaaacggg catgtaatgg gcgcaaacgg
     1021 ggtttctgca aaaaacgaac ggtacgagat actgtatatc aggagcgata tacaggaaaa
     1081 cagcgatatg atgaagtcgg tgatctcgct gctgaaattc ttcagcggga aagttcccgt
     1141 ggtcttctgc aggtctgatg agacgggaaa tattgctaga gcaaatatgg gcgaatatcg
     1201 tgtcgatgct tgtgatacac tgcaaagaga gctaaaggaa aggttcggag aagataacgt
     1261 caagctggtt tccaaggcga tgcagaaaca gcaatgatac gctgaaacgc tctaatacca
     1321 gcctttatcg gccgcctggc caaaatatca tgttgatttt tcaggtgaaa taatatatag
     1381 tagtctcgag gtggtttttt tggactggga gttggacaag cttataggag aattcattgt
     1441 agtcagggca gaagagaacg gagtaaacgt cataggtctt accagaggca aagacaccaa
     1501 attccatcat acggaaaaac ttgataaggg agaggttttg ctggcacagt ttacggagaa
     1561 tacttccgct ataaaagtga gaggaaaatc caggatatta tgccgccacg gtgagatcat
     1621 ttccggtgaa tagtccgaaa aggggcgtgt ttttatgtgg acagtagtat acatggctca
     1681 aagcaaggaa atagcgactg cactgcagga gttgctcact aaagaaggaa ttctcgtaaa
     1741 actgagacca atcagtaaaa atcacgataa cagcgacgat tattacgagg tacttgttcc
     1801 ggaggccgaa gtcgaagaag ctcacagcgt cattatcgaa aagggctatt gatggaattg
     1861 cgcaaactga taactcaagt gagacccgtg tataccatgg cacgggctta attttaaaga
     1921 gggatgtaag gaccgggagg aatcatgttc gtatcagaaa ctccattggt ggtaagatat
     1981 gcggaaacgg accagatggg cgtggtgcac cattccaatt acccgatatg gttcgaagcc
     2041 ggaaggacgg atttcataag gaaggtgggc atgccttatt cggtgataga gcagaaggga
     2101 gcgatgctgc cgctgctcga actgaggtgc cggttcatga gctttgccag gtatgaagac
     2161 aacataataa taaagacgta cataaaggaa tttacaggta cgaggctctg cttcagctat
     2221 caggccgtaa gggaagagga cggcaggata ctggccgagg gagagacgct gcactgctgg
     2281 accagaaggg atctgaaacc cgtaaacata aaaaaataca tgcctgaaat atatgaatta
     2341 atagaaaagg cagcaaaaga caattgatgt tcacgggccg tataattgta caatgacggt
     2401 tttatatgat aaactattct gtataagcga tataggcggt gaaacatgaa tatcccgaac
     2461 atactgacaa tattgcgctt tttgctgata cctgcatttg catattttct cagtgtacgc
     2521 agttacgtcg ttgcagtgct gctgtttctt gccggcgggc tgaccgatgt cctggacggt
     2581 tatatcgcca ggagatataa cctgataacg tcatggggga aaatagctga tccgatggct
     2641 gacaaactga tgcagataac ggcagtcgtc atattgtcgg cggtgctgaa gataataccg
     2701 atagaactgg tgctcgtgat ccttgcaaaa gagatcctta tgggcctggg ggcccttgtc
     2761 ctgtacaggc agaaaaaata cgtggtttct gccaactggt acgggaagat gaccaccgtt
     2821 attttttatc ttgccatcgt attgctgata ttcgatgagc ctttcggaag atggaccctg
     2881 cttggcgttg ccgttaacca gctggtattc atattggccg tggcagccac gttgttcgct
     2941 ttcttcatgt atttcaggtc atatctcaga atcaagcagc agaaacagta aagggatcct
     3001 atgttcggat atatcgttcc ggaaaaaccg gagatgaaaa taaaagagta cgagcttttc
     3061 agggcttatt actgcggagt ttgcagggcg ataggaaggc gctgcggcca gctgaaacgg
     3121 ctgacgctca gttatgacac tacgttcctg gccgtcctcc tgtcggcggc atccggtgag
     3181 aaagtacagg tcatgaaaaa gaggtgtgcc ctgcatcccc ttgaaaaaag acatgttatc
     3241 agcggcagtt ctgccgtgga ctatgcctca gacataaaca tcatactggc gtattataat
     3301 ctggaagata agaaacgtga caaagattcg ccggtttcag cgtccgcact ggttttgctg
     3361 aagggagctt tcaggcggct cagaaggaag tatcctgaaa agtgccgcat aatggaacag
     3421 cggcttgatg agctcgtccg gctggagaag cagcaatgcc cttcgatgga tatggccgcg
     3481 gagccgtttg caaggctgat ggaagaaata actgcgtatg agccgctgtg taccggcaag
     3541 gacaccgaga agatactcag gtggctcggc tataacctgg gcaagtggat atacctgctg
     3601 gacgcatttg acgatcttga gacggacata aaaagaggga attacaatcc gtttgtctgc
     3661 cagttcggat ataccggcca ggatgtggac ggattcagga atgagataag ggaaaaagcc
     3721 gggttcacgc tgttttacac gctcagcgag atagcccggg ctttcgaact gctggaggcc
     3781 aaaataaaca ggggcatact tgaaaatata atatatatcg gaatgctcag gaaaacagaa
     3841 aatattcttg gtataaggag atgcggcgaa gttgaagaat ccgtatgaag ttctcgggat
     3901 aagggaaggc gcaagcgagg aggaaataaa aaaagcatac agggaactgg taaaaaaata
     3961 tcatcccgac cagtacaggg acaatccgtt gtcgaaattg gcggaggaaa aacttgccga
     4021 gataaatgag gcttatgatt atcttatgaa aaacgtgtcc tcaggcggaa acagccagta
     4081 cagcggcggc gcatcgtacg gccgcagcgg ggccgacggc tggagcgaca gcgggagtga
     4141 ttttttcaac caggtgaagt cccatataaa cagcgggaat atcaggcttg cagaggaaat
     4201 gctggacagt tcgcgcagcc gtcccgccga atggtattat ctcaagggca tcatattcat
     4261 acgcaagggc tggtacaacg aagcatacac gcatatccag agggccgtca atatggatcc
     4321 ggccaattac gaatacagaa gcgcgcttga caggatgaac acgtcataca acgcataccg
     4381 gacgaacccg ttcaacagcg ggcgttacag gagcgaacct gatctgtgca ctatatgcca
     4441 gtgcctgtgg tgttccgact gttgctgcga atgtatgggc agcgatctga taagctgctg
     4501 ctgatattgg gaggaagtcc ggatgagagg tcccttcaaa tcaaaaacca ttgcattgaa
     4561 cggcgtgctg ggagctcttg cagtgatatg cctgctgctg gccgatgtac tgccgacaag
     4621 caagatatcc ttatatgtgc tgagttcctt tttcgtttcc gttgccataa tcgaaggagg
     4681 catccgggca ggcttgatat tttacgcagc caccgctctc gtcggattca ttatattgcc
     4741 tgacaagata agcatgatac cgtatgtgct cttcttcgga acatatggta tagtcaaatt
     4801 ttacattgaa aagctgaact gcattgtaat agaatatgtc ctaaaggtca ttttcttcaa
     4861 tgcctgcgcc ggcctggcac ttctggctgc cggaagcctg ttcgaagggg gacttgcgtc
     4921 cggtttgcca tgggggattc tcgcaggtat atgccaggtc atatttctgg tttatgatat
     4981 cgtgtatacc ttgttcataa actattacag ggacaggctc aggacgagaa tactgaagag
     5041 atgacctgaa aacagtgaca gtcaggggaa ttggatccaa tggaagaaag aacgcttcga
     5101 atcctggaat tcgttaaaat aaaagaaaaa ctggtatcac tctgctcttc cgaactgggg
     5161 aaagagctcg catctgttct tttgccgcag acacagcccg aagaagtgga gaggatgctg
     5221 aaagagacgt ccgacgcggt ggacttcata ctgaggaggg gaagccctcc cctgggaggc
     5281 atccatgata taagggacac gctcagaaga gtggaactcg ggactgctct caaccctggc
     5341 gaacttctca gggtggcaga tacactcagg gcagcccgca atttgaagaa ttatgcctcc
     5401 gaagccgacc cgcgtgcgga tgagagcggc aatcacatca ggaaccttat cgcgtccgtc
     5461 acgccgaaca agcgcatcga agacagcata aacgccgcga tcatcagcga tgaggaaatt
     5521 tcggacaatg caagcagcac gcttgcgagt ataaggaaaa gaatcaggga ggagcaggaa
     5581 tccctcaagg ataaactgaa ctcgatgatc cattcctcca aataccagaa gtttatgcag
     5641 gaatccatcg tcacgatcag ggaagaccgc tatgtgatcc cggtaaaggc tgagtacagg
     5701 aacgagatcc caggcctggt gcacgattcg tcagccagcg gggccacggt ctacatcgag
     5761 ccgatggctg tggtcgaagc caacaacaac ataaagcagc tcaggataaa agaacagctt
     5821 gaaatagaga ggatacttca ggaactgacc ggcgaggtcg caggcatcat cgaacccctg
     5881 aaggccaaca tgacgctgtt cgcacagttg gatttcgtat ttgcaaaagc aaagctgagc
     5941 cttgatctca aatgcgtatg cccgaaactg aacagggaaa aaaggatcaa tatcaaaaag
     6001 ggcaggcacc cgcttcttga cagcaaaacg gtcgttccca tcgacctttg gataggagag
     6061 aggttcacga ctcttgtcat caccgggccg aataccggcg gtaagacggt gacactcaag
     6121 accgtcggac tgtttaccct catggtccag tcaggactgc acgtcccggc tgcagaaggc
     6181 actgagatga gcgtattcga caatgtattt gcggatatag gcgatgagca gagtatcgaa
     6241 cagagcctga gcactttttc gtcccatatg agaaatatcg tggaaatatt gtcccgggcg
     6301 gatgatatgt ccctggtgct ttttgacgag cttggcgccg gtactgatcc tacggaaggc
     6361 gccgcacttg ccatgtccat actcgaaaac ctccacggca ggggagccat cactatcgct
     6421 acgacgcact acagtgaact gaaagtttat gcccttacca cggacggcgt cgaaaacgcc
     6481 tgctgcgaat tcgatgtgga gactcttagg ccgacataca ggctcctgat cggtgtgccc
     6541 gggaaaagca acgcatttgc catctcgaaa cgactggggc tttccgacga cattcttgac
     6601 agagccaggg agttcctttc cggtgaggaa attcagttcg aagacgtact gatgaccatc
     6661 gaaaggaacc ggagggagtc cgaacaggaa aggctgcagg ctgaaagtct caggctcgag
     6721 atagagaaac tgaagaaaga gctcgaagaa cagaaatata agctcagttc acaaagggaa
     6781 aaactcctgc gggaggcgag ggaagaggca agaaggatac tgctcgatgc aaggaaagat
     6841 gccggtgata tcctggagga aatgaagcag gcgacaaaac tgcaggatga aaaggagagg
     6901 agaagagcgg cagaagaagc aaaagcaaaa ctcaggggca gcatcgggaa gatcgaggag
     6961 tcgcttgcgg aaggcctttt ccggcggaaa ggcttcgtaa agccgccgga aaacc
//
LOCUS       DAOSCC010000128         7006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6927_length_7006_cov_4.238671, whole genome shotgun sequence.
ACCESSION   DAOSCC010000128 DAOSCC010000000
VERSION     DAOSCC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6927_length_7006_cov_4.238671"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLG72_07630"
     CDS             complement(<1..549)
                     /locus_tag="PLG72_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ80705.1"
                     /translation="MLSEIYLDNSATTRQYDEVTSLMAEVASKYYGNPSSLHTKGIEA
                     ERLVSGARETIASLLGAQPSEIFFTSGGTESNNLAIVGYLDANPRKGKHVITTKTEHP
                     SVLEVYRNLESRGYSVDYLDVDGNGRIIPGQLEQKIRPDTALISIILVNNETGTIQDI
                     NRISAIRDALNPHAVIHADAVQA"
     gene            789..2585
                     /locus_tag="PLG72_07635"
     CDS             789..2585
                     /locus_tag="PLG72_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ80706.1"
                     /translation="MLEFLKKNINLVCFFVLFSMVVSLSAFSYAQYISNNQDAGDRMQ
                     PFSDVPENSYAYEPIHRLRRMGVANGIGSNRFGYGRTISRGEFITLLVNLLGFDGEVP
                     QQGSFSDNKNPKKFYYKPVEIALEHDIISNETGAFRPDDPITRQEAAVMIVNGLGYAN
                     LAGRLSYFDAPYEDVSGYSGHITIARDFGIVSRASAFNPDRQLKREEAAAMLVRMLDV
                     MERKISGLNAFYAISSSSQSGMIPDLTSVCFGWSSLGYDSSSGNIVLNMSRTALGQND
                     YYLPSGFSSRLETAKNAGVPAYLMVYADQSSRIKDPVTGDVYGLPEYVFNDPATSEKV
                     ISEIADALEGISLGNETGSFDGVVVDFEGLKGESAKKGLNEFLRKLKEKLEPEGKKLL
                     VAVHPLMHPKRSASSMDGYDYRTIGVLADKVILMAHDYDAKRLTKSEAERGVTLTPLT
                     PIEDVYYALQAITDPVTGVQDKSKIMLQISFDWTVWKKQNGKTLNQVPLKYNLENFMK
                     LLDSGREISFFYHEDYENPYLKYTDSETGVEYTVWYEDKRSVLAKVKLARYFGIEGIS
                     LWRLGSIPDREDPGQNSPYMDVWQSILAEMDR"
     gene            complement(2590..2898)
                     /locus_tag="PLG72_07640"
     CDS             complement(2590..2898)
                     /locus_tag="PLG72_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ80707.1"
                     /translation="MKERRYVLKRHRYVLKNRRRFNIFLMTLTVLLFVLVFTVAANGA
                     DSEDNYETVVVKRGDTLWDFAREYRGDTDIRQYIEKIKTVNGLKDSTIYEGDIIRLPL
                     "
     gene            3066..3716
                     /gene="lexA"
                     /locus_tag="PLG72_07645"
     CDS             3066..3716
                     /gene="lexA"
                     /locus_tag="PLG72_07645"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ80708.1"
                     /translation="MSKKLTEKQQQILDFVNKQVEEKGYPPSVREICSAVGFKSTSTV
                     HGYLEKLRKNGLIAKDPTKPRAIRVIGGTRKAPRNENEGLKDFYPGKELVDVPIVGKV
                     TAGQPILAVENVEDTFPLPVDFVQNSTAFMLRVQGDSMIEAGILDNDLVLVKQQSTAN
                     NGEIVVALIGDEATVKTFYKEKDHIRLQPQNRYMDPIIVRDNIAILGKVIGVFRKM"
     gene            complement(3738..3977)
                     /gene="hfq"
                     /locus_tag="PLG72_07650"
     CDS             complement(3738..3977)
                     /gene="hfq"
                     /locus_tag="PLG72_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOJ80709.1"
                     /translation="MNKSNINLQDVFLNQVRKEHLPVTIYLTNGFQLKGMVKGFDNFT
                     VILDTDGRQQLVYKHAISTISPMKVVNFIFNDNKE"
     gene            complement(4046..4990)
                     /gene="miaA"
                     /locus_tag="PLG72_07655"
     CDS             complement(4046..4990)
                     /gene="miaA"
                     /locus_tag="PLG72_07655"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ80710.1"
                     /translation="MEPVIVIAGPTASGKTRLSIELARLINGSVVSADSMQIYKYMDI
                     GTAKPGKEEMSGIRHYMIDEVEPDENYSVARYRERALECIADIIREGRRPIIAGGTGL
                     YINSLIYNINFSETICDEDLRKALKAEAEQKGNRYLYEKLVEIDPEAAERIHENDIKR
                     IIRAIEVYTHTQKTISEHIRMSRIEPPPYRYVVFGLNWDREELYRRINKRVDNMIRDG
                     LVEETRHLVEMGYDKRSTAMQAIGYKEILPYLRGECSLEEAVEILKRNTRRYAKRQMT
                     WFRQIREIIWLDMDEDTDYKETAEKIINHCIETFGIIL"
     gene            complement(5041..>7006)
                     /gene="mutL"
                     /locus_tag="PLG72_07660"
     CDS             complement(5041..>7006)
                     /gene="mutL"
                     /locus_tag="PLG72_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ80711.1"
                     /translation="VSKVQMLTRTHDSVQGICVTVAGGSINEIRPAGCPAGTSITVNE
                     LFFNTPARYKFLKKDAAEAGYAADILTRIALSHPGISFTFTNNGRKVMHTPGNGDLKS
                     TIFSIYGAETAKYLAKVDYEDKGIKLDGYAGLAEIARSSRVNQSIYINKRYIRNKTVT
                     SAIDAAYSTFLMKNRYPFIVLNIGMDPSKVDVNVHPSKMEVKFSNEQDIFRAIYHAVN
                     NALLSQSAVRTLSGTGRSAGNDSITVNSITVNGMVTERTHPVLQPGSGPFSGWTPQKG
                     KAVSGRSSYTAQYTQQEFELVKKELDLKYQEAISGRYQEQDAGTVEYSANGMVPSKTS
                     GYQADGPKKDIQDGPSMNRTDTVNKADRQAGIYEDKRDGTGINEDAGFVKDAAFGLFT
                     EQRETSEGTLAGIEKENSVTETAKPGDRCADQQVMLKKEAADNICKTVHTDGDVKYMD
                     GRTGDLPDISYLFKNTRIIGQLFSTYILLEGADELFLLDQHAAHERIRYEKLKKAYDQ
                     NRPLSQILLSAIHVDLTSAEYEYAMGEKSFFERLGFTFESFGTNSVIIRSVPLIDDGM
                     DVRECFTETLDFIMYHDVTDKSLVADEALYRMACKSAVKANRKLDEREINALLADLSA
                     LENPYTCPHGRPAIYRIRRYDLEKLFKRIV"
BASE COUNT         1706 a         1701 c         1593 g         2006 t
ORIGIN      
        1 agcctggacc gcgtccgcat gtatgacggc atgcgggttc agcgcatccc tgatggccga
       61 tatcctgttt atatcctgta tcgttcctgt ctcgttattg acgagtatga tgcttataag
      121 cgccgtatca ggccttattt tttgttcaag ctgcccgggt attatccggc cgttcccgtc
      181 tacatcaaga taatccacgc tgtaacccct gctttcaaga ttcctgtaca cttcaagcac
      241 ggatggatgc tctgttttgg tggttataac atgtttccct ttcctggggt tcgcgtcaag
      301 atacccgact atggcaaggt tgtttgactc ggtgccgccc gatgtgaaaa aaatctccga
      361 aggctgggct cccagcaaag atgctatggt ttccctggcg ccggagacaa gcctttcggc
      421 ttctatgcct ttcgtatgca gcgacgacgg attgccgtag tattttgaag ctacttcggc
      481 catcagcgaa gtgacttcgt catattgtct tgttgttgca ctgttgtcca gatatatttc
      541 ggataacatt ccatacctct tcaaacttgc atgtccggaa cttaattcgt ccgggactta
      601 tgcaattcct gctattgccc gttgcgtttt tctatagatt attatgtcca ataccagccg
      661 cctgaaaaat tatccatatg gctgaccctt cctattatag cacactctta tctaaatgtt
      721 gcagttattc gtcccgttat gataaactta tactgaatta tgtcaaatta cggttttcag
      781 ggggcaaaat gctggagttt ttaaaaaaga atataaatct ggtttgcttt tttgttctgt
      841 tctcaatggt ggtatcattg tccgcctttt catatgccca gtatatttcg aacaatcagg
      901 atgcgggcga caggatgcag ccgttttccg acgtgcccga aaacagctac gcatatgaac
      961 cgatccacag gctcaggcga atgggcgtag caaatggtat tggaagcaac aggttcggtt
     1021 acggccgtac gatatcgagg ggtgaattca tcactctcct ggtgaacttg ctgggctttg
     1081 acggagaagt cccgcagcag ggttcgtttt cagacaataa aaacccgaaa aagttttact
     1141 ataaacctgt cgaaatcgca cttgagcacg atataatctc aaatgaaacc ggcgctttca
     1201 gaccagatga tccgatcacc cgccaggaag ctgcagtaat gatcgttaac ggcctcggtt
     1261 atgcgaatct tgccggcagg ctcagttatt tcgatgcccc gtacgaagat gtgtccggat
     1321 attcaggcca tatcactata gcaagggatt ttgggatagt aagcagggca tccgctttca
     1381 atcctgacag gcagcttaag agagaagagg cagcagcgat gctggtgagg atgctggacg
     1441 taatggaaag aaaaatcagc gggcttaacg ctttttatgc aataagctct tcatcccaga
     1501 gcggcatgat ccctgatctc acttctgtat gcttcggctg gagcagcctg ggatatgaca
     1561 gttcttccgg aaacatagtc ctcaacatgt cacgtacagc gctgggacag aatgattatt
     1621 accttccctc aggattcagc agcaggctgg aaacagccaa aaacgccggc gtacctgcat
     1681 accttatggt ttacgcggat cagtcgtcca ggatcaaaga ccctgttaca ggagatgtgt
     1741 acggtcttcc tgaatatgta ttcaatgatc cggcaacatc tgaaaaagtc atctctgaaa
     1801 tagccgacgc tctcgaaggc atttccctgg gcaacgagac cggttcgttc gacggtgtgg
     1861 tcgtcgactt tgaaggtctc aaaggcgaaa gtgcgaagaa aggactcaac gagtttctga
     1921 ggaagctgaa agaaaagctg gaacccgaag ggaaaaagct tcttgttgca gtacatccgc
     1981 tgatgcatcc aaaacggagt gcttcaagta tggacgggta tgactacagg acgatcggcg
     2041 tgcttgctga caaagttata ctgatggctc atgactatga cgccaaacga cttaccaaat
     2101 ctgaggcgga acgcggcgtc acgctcactc cgctgactcc gatcgaagat gtgtattacg
     2161 cgctccaggc catcaccgat ccggtaaccg gggtacagga taaaagcaag atcatgctcc
     2221 agatatcatt tgactggacc gtctggaaaa agcaaaacgg caaaacactg aaccaggtgc
     2281 cgctgaaata caaccttgag aacttcatga agctgctgga cagcggcagg gagatatcgt
     2341 tcttttacca tgaagattat gaaaatccat atctgaaata taccgactcc gaaaccggcg
     2401 tcgaatacac agtctggtat gaagacaaaa ggagtgtact ggctaaggta aagcttgcaa
     2461 ggtacttcgg cattgaaggc atatcactgt ggaggcttgg atcgataccg gaccgcgagg
     2521 atcccggaca aaacagtcca tatatggacg tatggcagtc gattctggca gagatggaca
     2581 ggtaattcgt caaagaggga gcctgataat gtccccttcg taaatagtgc tgtctttcag
     2641 gccatttact gtttttatct tttctatgta ctgccggatg tcggtgtcgc cccggtattc
     2701 tctagcaaag tcccagagag tgtctcccct ctttacaacg actgtctcat aattatcttc
     2761 ggaatccgcg ccgttggcag caaccgtgaa aaccaggaca aacagcagaa ctgtcagcgt
     2821 catcaggaat atgttgaaac gtctgcggtt tttaagaaca tatctgtgcc ttttaagtac
     2881 atatcttctt tctttcatat aatcatgtca ccacctacag aacacatgtt ctgttcagat
     2941 tatacacgaa caggtgttct gtgtcaatat atatttcgaa catttgtttg acattttttt
     3001 gtttttttaa tataatggga tcagtggata aaaatactgg gtaaaagtca aaaggggtgt
     3061 ataggatgag caaaaagctt actgaaaagc aacagcagat cctggatttc gtcaacaagc
     3121 aggtcgaaga gaagggatat ccgccgtcag tgcgtgagat atgcagcgca gtcggattca
     3181 aatccacttc caccgtgcac ggctatcttg aaaaactgag aaaaaacgga ctgatagcca
     3241 aggatcctac gaaaccgaga gccatcaggg tcattggagg aaccaggaag gcaccccgaa
     3301 acgaaaacga aggattgaag gacttttacc cgggaaaaga actggtcgac gtgccgatag
     3361 tgggcaaagt cacagccggc cagcctatcc tcgcggttga gaatgttgaa gacaccttcc
     3421 cgctgcccgt agattttgtc cagaattcca ccgcattcat gctgagggtc cagggagaca
     3481 gtatgatcga agccggcata ctcgacaacg accttgtcct ggtaaaacag cagtccactg
     3541 ccaacaacgg agaaatagtc gtggctctca tcggcgatga agcgaccgtc aagacatttt
     3601 acaaggaaaa ggaccatata aggctccagc cccagaacag gtacatggat cccatcatag
     3661 tccgtgataa cattgctata cttggcaagg tgataggcgt cttcagaaag atgtagcagg
     3721 tctgatgacc cttgcgttta ttccttgttg tcgttgaaaa tgaagtttac cactttcatc
     3781 gggcttatgg tggaaattgc gtgtttataa accagttgct gccttccgtc ggtatccagt
     3841 atgacagtaa agttatcgaa tccctttacc atgcccttga gctggaaacc gttcgtaagg
     3901 taaatggtca ccggaaggtg ctcttttctg acctgattta aaaatacatc ctgcaaattg
     3961 atatttgatt tgttcacctg acaatcctcc tcgatcatat atcctcactc ctgaatcatc
     4021 tccaaaagta aggatcattt taattctaca agattattcc aaacgtttca atacaatgat
     4081 taattatttt ctctgcagtc tctttgtaat cagtatcttc atccatgtcc agccagatta
     4141 tttcccggat ctgcctgaac cacgtcatct gccttttggc ataccgcctc gtgttacgtt
     4201 tcaggatttc aacggcttcc tccagtgagc actctcctct cagataaggc aatatttcct
     4261 tgtatccgat agcctgcatc gcggtgcttc ttttgtcata tcccatttcc accaggtgcc
     4321 tggtctcttc cacaagtccg tccctgatca tattgtcgac ccttttgttt atgcgccggt
     4381 acagttcctc cctgtcccag ttgagcccga aaacgacgta cctgtacggc ggcggttcga
     4441 tcctggacat ccttatatgt tctgagatcg ttttttgggt atgtgtgtat acctctatgg
     4501 ctcttatgat ccttttgata tcgttctcat gtattctttc cgccgcctca ggatcgatct
     4561 caacgagctt ctcataaagg tatctgttcc ccttctgctc tgcctcggcc ttcagggctt
     4621 ttctcaggtc ctcgtcgcat attgtctcag agaagttgat gttatagatc agagagttaa
     4681 tgtataagcc tgttccgcct gcaatgatcg ggcgacggcc ttccctgatg atatccgcga
     4741 tgcattcgag agcccgttcc ctgtatcttg cgacgctgta attttcgtcc ggttccacct
     4801 cgtcgatcat gtagtgcctg attcctgaca tttcttcctt cccaggtttg gctgtgccga
     4861 tatccatgta tttgtatatc tgcatcgagt cggcggaaac aacgctgccg tttatgagcc
     4921 tggccagttc gatggacaaa cgggtctttc ctgacgctgt aggacctgct attacgatta
     4981 caggttccat cccggccatt ccccctccta tctgacaatt gtaaccagca gtaatattat
     5041 ttatactata cgtttgaaca atttttccag gtcgtatctc ctgatcctgt atattgcagg
     5101 acgtccgtga ggacatgtat acgggttttc caaagccgac aggtcggcca gcaacgcgtt
     5161 tatttccctc tcatccagtt ttctgttggc tttgaccgct gatttgcatg ccatcctgta
     5221 aagagcttca tcggcaacga ggcttttgtc tgtaacgtca tggtacatga tgaagtcaag
     5281 cgtttccgta aaacattccc ggacatccat gccatcgtcg atcagaggca ctgatctgat
     5341 tatgaccgaa ttcgttccga agctttcaaa agtaaagccc agtctttcaa aaaaagattt
     5401 ctcacccatt gcatattcat attcagcact tgtcaggtcg acatgtattg ctgacaacag
     5461 tatctgtgat aaaggcctgt tctgatcata tgcctttttg agtttctcgt accttatcct
     5521 ttcgtgggcg gcatgctgat cgagcaggaa aagttcgtcg gcgccctcaa gaagtatata
     5581 ggttgagaac agttgcccga ttatccttgt atttttaaag agatatgata tatcgggcaa
     5641 atcgcctgtc ctgccatcca tgtattttac atcgccatcc gtatggacgg ttttgcaaat
     5701 attatccgct gcttcttttt tcagcatgac ctgttggtcc gcgcatctgt cgcctggttt
     5761 tgcagtttca gttacactgt tttctttttc aatacctgcc aaagttcctt ctgatgtttc
     5821 gcgctgttcc gtaaacaggc cgaatgctgc atccttcacg aaccctgcgt cttcgtttat
     5881 gcctgtaccg tcccgcttat cttcatagat cccggcctgc ctgtcagcct tattcacggt
     5941 atcggtccgg ttcatggaag gtccatcttg aatatccttt tttggtccat ctgcctgata
     6001 accggatgtt ttcgacggta ccatgccatt tgcgctgtat tcaacagtgc cggcatcctg
     6061 ctcctggtac ctgccggata ttgcctcctg gtatttgagg tccagttcct tttttaccag
     6121 ttcgaattcc tgctgggtat attgcgccgt gtatgatgac cgtccggaca cagcttttcc
     6181 cttctgaggc gtccatccgg agaatggccc tgatccgggc tgtaataccg gatgtgttcg
     6241 ttcagtgacc ataccattta ctgttatgct atttactgtt atgctatcgt taccggcgct
     6301 tctcccggta cctgaaagcg tcctgacagc agactgcgac aaaagggcgt tgttgacagc
     6361 gtgataaatc gccctgaaaa tatcctgctc attggagaat ttgacttcca tctttgacgg
     6421 atgtacattg acgtctactt tcgacggatc cataccgata ttgagcacga tgaacgggta
     6481 cctgtttttc atcaggaaag tcgaatatgc cgcatcgatg gcagacgtga cggtcttatt
     6541 cctgatgtat cttttattta tgtatatcga ctggtttacc ctgcttgaac gggctatttc
     6601 ggcaagtccg gcatatccgt caagttttat gcctttgtct tcataatcta ctttcgccag
     6661 atattttgcc gtttccgcgc cataaatgct gaaaatcgtg cttttaagat ctccgttgcc
     6721 cggcgtgtgc atcactttcc tgccgttgtt cgtaaatgtg aaagatattc cgggatggga
     6781 cagcgctatc cgggtaagta tatctgccgc atatcctgct tccgcggcgt ctttcttaag
     6841 gaacttatac cttgccggag tattgaaaaa caattcgttc accgttatgg aagtacctgc
     6901 gggacagccg gccggcctga tttcattgat agaaccgccg gctaccgtta cacagatgcc
     6961 ctgtacgctg tcatgggtcc tggtaagcat ctgtactttc gaaaca
//
LOCUS       DAOSCC010000129         6999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_6934_length_6999_cov_4.560916, whole genome shotgun sequence.
ACCESSION   DAOSCC010000129 DAOSCC010000000
VERSION     DAOSCC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_6999_cov_4.560916"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1201
                     /locus_tag="PLG72_07665"
     CDS             155..1201
                     /locus_tag="PLG72_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOJ80712.1"
                     /translation="MDNGVIDMKKYEEFLQSHPKGHFMQSPQWAEVKSFWKNEIVTVE
                     DENGNIKGSMSLLIRKIPILPYTMMYSPRGPVCDPHDGDTIKKLLEKCKALAKKYRSY
                     VLKMDPDIEIEDTQFEQIVRKLGFKVSRGLKNYEGIQPRFVFRLDLRGKTEEQIMADF
                     HHKVRYNIRLAERKGVEVRVGTREDMAVFHRMIVETGKRDNFVVRSREYYEKVYDCFA
                     PDHARVYIASYEGKPIAGTVAILYGNKCWYLYGASINEHRNLMPNYLLQWNMIKWALE
                     SGCDIYDFRGVPGDLDENNPMYGLYRFKVGFKGKFTEFVGMLDYVFDPFVYFIAEKAI
                     YAFRELRRKIYKRK"
     gene            1208..2899
                     /locus_tag="PLG72_07670"
     CDS             1208..2899
                     /locus_tag="PLG72_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOJ80713.1"
                     /translation="MKKQSSVKGFTVLSAASIINKLLGLLYVPVVTLIIGNYGNGIRE
                     QGYKLYELAFVITNTGIPVALSKIISEQLASSRYKLSYRTLRVSAVLMTAGGLFTSIL
                     TAALAGPVSKLMQTPQAYWTIMALSPALLFTSISCIFRGYFQGRSNMVPTSISQIIEQ
                     VINTVLAVLFAWLAYQWGLKFAAGEGLAGEEMVNEALKFAAAGATVGVSAGAMASAGY
                     LYWTYARIKAEIIDEFRYSSTDGSEMSATDILKWIVRLALPITLGQVMIYVTQLIDAV
                     NTKSRLMTAGFSDVEATSMYGVLATQYTKVLFVPVVFATALSTAVLPSISEAAAVNDR
                     ALLNRRISEAFRTIFMIAVPAATGLTVMAKPVINILFFRSPDGWDLLMAGSWTIILIS
                     LVSVQTSVLQGLGKTYVPTVHMVIGLALKLIVNYTLIAVRSINIKGAIIGNAVCYIFA
                     AVMNYRALKKLTGIRLNVRKTFNRPLSVSIIMGIFVYLIYHGFVFMLEWLIKTVFVLN
                     AVCCFAAVAAGCLIYYMLMIVAGGITADDIRNLPMGEKILAVTAKFPYVDRFLMR"
     gene            complement(2979..4355)
                     /locus_tag="PLG72_07675"
     CDS             complement(2979..4355)
                     /locus_tag="PLG72_07675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ80714.1"
                     /translation="MKKGLLELLYLFVITILSFTLAVPVYAKSPDIPDEETCYVLMDA
                     KTGRIFAEQNADKQIRPASLTKILTAIVALENGDLQQEMKVSQEAVYDIGRGGMNIGI
                     MAGETGFTLENMLNVMLIKSANEAANIIAENVASTRKDYVEMMNRRAKELGAVNTYYV
                     NPSGKDTEKEDADHFTTARDIAVMARHAMTIPEFRKIVATEYYEDLPATNKHDKWDPL
                     RNTNKLLWYDNTYPYTVSGKECEYTVTGMKTGYTAAAGNNLVISATGEDGMELIAVVM
                     HVMTANKVYGYATELLRYGFENYSMQKLTDAGSTVKSVIVEEAAVNDNILDLVTGSEF
                     SIALPVQSDGTAGNDIETRLDIPNVIKAPVRKGDVIGTVEYISDGKAIGKVDLIAART
                     ISKAPVAAAQDTEAEDPGLESHYSYTVLGILLAASGFVILRTILKKISRRVNKKKYEY
                     RYYRGPNS"
     gene            4822..6474
                     /locus_tag="PLG72_07680"
     CDS             4822..6474
                     /locus_tag="PLG72_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ80715.1"
                     /translation="MKIRRIVRILAIAVIIAVAVSIYLQEKNVIEREDSYGYVQSSVK
                     SDEGKIRVVISAVGDVVLGQDSRFSYRDSFDHVFEKTGGDYGYFFSNAREVLEQDDIT
                     IANLECVLGNETEKAEKYDYGNNYWFIGRPEYVNILKAGSIEAVSLANNHTYDYGQKG
                     YDATCRALDDVGIKHFGYSRTAVSMVNGINVGMAGFNQLGEYEQGRDTDELKQEIESV
                     TRDLRERSNLVVVYFHWGKEYSYKTDRLQTELAQLAIDSGADLVLGSHPHVLQPIEIY
                     KDRYIVYSLANFCFGGNKKPSDLDTAVYRQAFLFDRDGSLVSIQAPEIIPFSASSQRS
                     VNDYRPTPAEGEAKDRIFAKLDYSADMAALMELSVDKDEMVRLDEVAEDVIIDLKYAS
                     SDNITGKPVYDSNTAWLRRGTANKLKKANEALMNQGYRIKVWDAYRSEEDHRILHDTA
                     KNSYYFIDPKIGSNHTRGAAVDVTLVDIDGNELDMPSRFDEMSEKAHRTYKFATPEQK
                     RNAVILEKAMKDAGFIPLENEWWHFDDSECKSYEFLPSLNDE"
BASE COUNT         1750 a         1655 c         1892 g         1702 t
ORIGIN      
        1 acaggatcat gaggatgctg ttcctgtata aatcacatct gagccactgg ttcaaattca
       61 ggaaatacct gtgacctgct gaaccggcga atgacccggt cacacggaac aaacgggata
      121 aggctatacg gaataaacga cagaggtgac gcaattggat aacggagtta tcgatatgaa
      181 gaaatacgag gagtttttgc agtctcatcc gaaaggccat ttcatgcagt ccccgcagtg
      241 ggcggaggtg aaatccttct ggaagaacga gatcgtgacc gtggaggatg aaaacgggaa
      301 tataaagggt tcgatgagcc tgctcatcag gaagataccg atacttccgt ataccatgat
      361 gtactcgcct aggggccccg tttgcgaccc tcatgacggg gacacgatca aaaaactgct
      421 tgagaagtgc aaggctctgg cgaaaaagta ccgcagctat gtcctgaaaa tggaccctga
      481 catcgagatc gaagatacgc agttcgaaca gatagtaaga aaactcgggt tcaaggtgag
      541 ccgcggtctt aagaactatg agggcatcca gcccagattc gttttcaggc tcgatctgcg
      601 cggcaagacc gaagagcaga tcatggccga cttccatcac aaggtacgct acaatatcag
      661 gcttgcagag aggaaaggcg tggaagtcag ggtaggtacc agggaagaca tggccgtttt
      721 ccacaggatg atcgttgaga ccggaaaacg cgacaacttt gtcgtcagga gccgggagta
      781 ttatgaaaag gtttacgatt gcttcgcccc ggaccatgcc agggtctaca tcgcgagcta
      841 tgagggcaag cctatcgcgg gcactgtcgc catcctttac ggaaacaagt gctggtacct
      901 gtatggcgcg agcatcaatg agcaccgcaa tctcatgcct aattaccttt tgcagtggaa
      961 catgataaaa tgggcactgg aatcaggctg cgacatatat gatttcaggg gcgttccggg
     1021 agacctggac gagaacaacc ccatgtacgg cctttacagg ttcaaggtcg gattcaaagg
     1081 taagttcacc gagttcgtgg ggatgcttga ttatgttttc gacccgttcg tatacttcat
     1141 agcagaaaaa gccatttatg ccttccgtga actcaggaga aaaatatata agagaaagta
     1201 aagaacagtg aaaaaacaat cgagtgtaaa aggctttacc gttttaagcg cggcaagtat
     1261 cattaataag ctgcttggcc tgctgtatgt gcccgttgtt acgctgataa tcggcaatta
     1321 tggcaatggt atacgcgaac agggctataa gctttacgag ctggcatttg tcataaccaa
     1381 taccggcata cctgtcgcgt tgtcaaaaat catatcagaa cagctggcgt catcaagata
     1441 taagctttca tacaggacgc tgcgtgtatc cgccgtactg atgacagcgg gaggactctt
     1501 taccagcata cttaccgctg ccctggccgg ccctgtgtcg aaactgatgc aaactccgca
     1561 ggcatactgg acgatcatgg ccttgtcccc cgccttgctt ttcacttcca tatcctgcat
     1621 attcaggggc tatttccagg gcaggtccaa tatggtgccc acaagtatat cacaaataat
     1681 agaacaggtc attaataccg tgctggctgt attgtttgcc tggcttgcgt accagtgggg
     1741 actgaagttt gccgcaggag agggactggc aggcgaagag atggtcaacg aggcgctgaa
     1801 atttgcggcg gcaggcgcca ctgtaggcgt atctgccgga gcgatggcaa gcgccggata
     1861 cctgtactgg acttatgcca ggattaaggc tgagatcatc gatgaattca ggtattcgtc
     1921 gactgacgga tccgagatgt cggccacaga tatccttaaa tggattgtaa gactggcttt
     1981 gcctataaca cttggacagg taatgatata tgtgacgcag ctgatagatg cggtaaatac
     2041 caaatcgaga ctgatgactg ccggtttcag cgatgttgag gccacaagca tgtacggcgt
     2101 cctggcgacg caatacacaa aagtgttgtt tgttcctgtc gtgtttgcga cggcgcttag
     2161 tacggccgta ctcccgtcca tatcggaggc agctgcagtc aatgaccggg ctttgttgaa
     2221 caggaggata tcagaagctt tcaggaccat attcatgata gcggtacctg ctgctacagg
     2281 ccttacggtc atggcaaagc ctgtaataaa tatacttttc ttcagatcgc ctgatggctg
     2341 ggatctgctg atggcaggtt cgtggacgat catcctcatt tctctggtat cagtgcagac
     2401 ctcggtactt caaggcctgg ggaagactta tgtgccaacc gtacatatgg tgatcggtct
     2461 tgccctgaag cttatcgtga actacacgct gatcgctgta agatcgataa acatcaaggg
     2521 cgccatcatc ggtaacgccg tatgctatat ctttgcggca gtcatgaatt acagggcgct
     2581 gaagaagctg acaggcatca ggctgaatgt caggaagact ttcaacaggc ctctgagcgt
     2641 gtcgatcatt atgggcattt ttgtgtatct gatttatcac gggttcgtat tcatgctgga
     2701 atggctcatc aaaacagtat tcgtattgaa tgctgtttgc tgctttgctg cggttgcagc
     2761 cggctgcctg atctactaca tgcttatgat cgttgccggc gggatcacgg cagacgacat
     2821 ccggaacctt ccaatgggag aaaagatcct ggcggttacc gcaaagttcc cttacgtaga
     2881 cagattcctg atgcgataac ctgcagctgc cagataccag gacctgcgtt tgccgggcac
     2941 aggaacctgc ggctgcccgg tatggcggcg gcagggcctt agctgttcgg ccctcggtaa
     3001 tacctgtact cgtatttttt cttattgacc cttctggata tctttttcag tattgtcctg
     3061 agtatgacga aaccgcttgc tgcaagcagg atgcccagca cggtatatga atagtgcgat
     3121 tcgagccccg ggtcttcggc ttccgtatcc tgggctgctg ccacaggggc tttgcttatc
     3181 gttcttgcgg caatgaggtc tacttttcca atcgcctttc cgtccgagat atattcaaca
     3241 gttccgatca catcgccttt tcgtaccggc gccttgatca cattgggtat gtccagcctg
     3301 gtttcgatat cgttacctgc agtaccgtcg gactgcaccg ggagtgcgat actgaactcc
     3361 gatcctgtta ccaggtccag tatattatca tttacggctg cctcttctac aatcaccgat
     3421 tttactgtgc ttccggcatc cgtcagcttt tgcatcgaat agttttcgaa accgtaacgg
     3481 agcagttccg tagcataccc gtagaccttg ttcgccgtca tcacgtgcat gactactgcg
     3541 atcagttcca tgccgtcctc gcccgttgcc gatatcacga gattgtttcc tgcagccgcc
     3601 gtatagccgg ttttcatgcc tgtcacggta tactcgcatt ctttgccgct gaccgtgtat
     3661 ggatatgtat tgtcatacca cagcagtttg ttcgtgttcc tcagcggatc ccatttatcg
     3721 tgcttgtttg tggcgggcag gtcttcatag tattccgtgg ccacgatttt cctgaattcg
     3781 gggatggtca tggcgtgtcg ggccatgacc gcgatatcgc gggcggtcgt gaaatggtcg
     3841 gcgtcttcct tttctgtgtc ttttccggaa gggttcacgt aatatgtatt tacagccccc
     3901 aattcctttg ctctccgatt catcatttcc acgtaatctt tgcgtgtaga ggccacgttt
     3961 tcggcaatga tattggcagc ctcgttggcg gattttatca gcatcacgtt gagcatgttt
     4021 tccagtgtaa agccggtctc gcccgccatg atgcctatgt tcatcccgcc cctgcctata
     4081 tcgtatacgg cttcctggga tactttcatc tcctgctgca ggtcgccgtt ctccagcgcc
     4141 acgatggcgg taagtatctt ggtgaggctg gcaggacgta tctgcttgtc cgcattctgc
     4201 tccgcaaaaa tccttccagt ctttgcatcc atcagtacat aacaggtttc ttcgtctggt
     4261 atgtccggcg acttggcata cacgggcaca gcaagggtaa aagacaggat agttatgaca
     4321 aacaggtata acagttccag taatcctttt ttcatatgtc tctccagttt ggtcagtctt
     4381 ttgtgaaata gtgttcgctt tgcgagaccg catcatgcct gttgcaaaat tgcatcattt
     4441 tcattttagc attttgcggc gcccgtttca agccgcacca gcacgggcat ccgcggaaac
     4501 atacaggtca gcccttctct gcccgccgca gcttggcgca gaacatgtat taacgtcctt
     4561 catggtacgc cgggtatatg gcggcattac aatgcatatg cgcctgggtt gtcgtgcgtg
     4621 tatcggttat atgtagtaca atagtaatat aatgccgata ttatagctgg gcaatgcaaa
     4681 tgctgcgcca gtgcaatggc aatgctgaac agtgcagata ctcgtgttgg tatgatgatg
     4741 tgcgatgctt atacataaat tcaccgggaa gtggcaaatt aagtataaac aatcaaccag
     4801 tgccgggagg atcatttact aatgaaaatc agacggattg tcaggattct ggcgattgca
     4861 gtcatcattg ccgtcgctgt ttcgatatac ctgcaggaga agaatgtgat agaaagagag
     4921 gacagttacg ggtatgtcca gagttctgtc aaaagcgacg aagggaaaat cagggtagtc
     4981 ataagcgccg taggcgacgt ggtgctcggg caggactcgc ggttcagtta cagggattcg
     5041 ttcgatcatg ttttcgaaaa gaccggcgga gattacggtt actttttctc caacgcccgg
     5101 gaggtactcg agcaggatga catcacgata gcgaatcttg agtgcgtcct gggcaatgag
     5161 acggaaaagg ctgaaaaata tgactacggc aataattact ggttcattgg caggcctgaa
     5221 tatgtgaaca tcctgaaggc cggaagcata gaggctgtca gtctcgcaaa caaccacacg
     5281 tatgactatg ggcagaaggg atacgatgct acctgcaggg cccttgacga cgtggggata
     5341 aagcatttcg gctattcccg gacggcggtc agcatggtca acgggataaa tgtcggcatg
     5401 gccggattca accagcttgg cgaatacgag cagggccgtg acaccgatga gcttaagcag
     5461 gagatcgaat ccgtgaccag ggatctcagg gagcggagca atcttgtcgt tgtttacttc
     5521 cactggggca aagagtattc ttacaaaacg gacaggctgc agacggagct ggcgcagctt
     5581 gccatagatt ccggggccga ccttgttttg ggcagccatc cccacgtatt gcagccgatc
     5641 gagatataca aagacaggta tatagtctac agccttgcca atttctgctt cggcggcaac
     5701 aaaaagccgt cggatctcga tacggcggtc taccggcagg catttctgtt cgaccgggac
     5761 ggcagcctgg tcagcataca ggcgccggag ataataccgt tctcggcatc atcgcagcga
     5821 tccgtaaacg attacaggcc gacgcctgct gaaggggaag caaaagaccg gatttttgca
     5881 aaactggact attcggcgga tatggcggca ttgatggaac tgtcggtgga taaggatgaa
     5941 atggtcaggc ttgatgaggt ggcagaggat gtaatcattg acctgaagta tgcctcgtcg
     6001 gacaacatca cgggaaagcc cgtctatgac tcgaacaccg cctggctgag aagaggtact
     6061 gcaaacaagc tcaagaaggc caacgaggcc ttgatgaacc agggttacag gatcaaggta
     6121 tgggatgcgt acagatccga ggaggatcac aggatcctgc atgatactgc gaagaacagc
     6181 tattacttca tcgacccgaa gatcggctcc aatcatacac ggggggccgc agtcgatgtg
     6241 acgctggtgg atatcgacgg gaacgagctc gatatgccca gcagattcga cgagatgtcc
     6301 gaaaaagcac acaggactta caagtttgcc acgcccgaac agaaaaggaa tgccgtgata
     6361 ctggagaaag cgatgaagga tgccgggttc atcccgctgg aaaacgagtg gtggcatttt
     6421 gacgactcgg aatgcaagag ctatgagttc ctgccttcgc tgaatgatga ataagctgag
     6481 gtttaatatc gcggctttgg gtatgtgaac aagcggttcc cggagcgcat gataacaggc
     6541 gccgtttgag cggaagcagg accagggatt gcctgctata taccgggatc cccgcggcag
     6601 ccggatgtac gactcgattc tggtggaaaa acggacaagc cggtgataaa ataatgtccg
     6661 gcaataatgc aggccggttg ggcagaccgg cgccgaatat ttatacatgc caggcgggaa
     6721 attgaaataa taattaagta agcgttatca tcgtacaggt gaacgctatc agttgctccg
     6781 gcaggctgct tcacaccggg tcccggaagc agcggccggc aagtcacggg ggtgcgggat
     6841 ggaaaaacat gctttggagg agtacataga aaaactccag gagaaaaaac tgctgctgag
     6901 gcacaactgt acgcttgaaa ccgcaaaaca ggcagtgaac catgtggcgt acgattcgaa
     6961 caaggcagcg cgcaatacgc ttttcgtatg caagggagc
//
LOCUS       DAOSCC010000130         6910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7048_length_6910_cov_4.585996, whole genome shotgun sequence.
ACCESSION   DAOSCC010000130 DAOSCC010000000
VERSION     DAOSCC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7048_length_6910_cov_4.585996"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLG72_07685"
     CDS             <1..455
                     /locus_tag="PLG72_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOJ80716.1"
                     /translation="KGTLKDVIVGADVFIGLFVAGVLTKDMVRSMAKDPVIFAMANPT
                     PEIMPDEAIEAGAKVIGTGRSDFPNQINNVLAFPGIFRGALDVRARDINDEMKIAAAQ
                     AIAGLISDDELNPEYVIPAPFDPRVSKAVAKAVADAAIRTGVNRIGGR"
     gene            552..1748
                     /locus_tag="PLG72_07690"
     CDS             552..1748
                     /locus_tag="PLG72_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ80717.1"
                     /translation="MNILLVIIYLAFISLGLPDSLLGSAWPAMRADLGAPVSTAGWLA
                     MTISVCTIISSLFSNRVITRFKTGKVTAVSVAMTAVALFGFAFAPSVWSMFLMAIPLG
                     LGAGSIDAGLNNYVALHYESSHMNWLHSFWGIGATVGPMIMAYHIAAQNGWRYGYLTI
                     AAVQAVLVAVLFASLKLWKNGADTSSSDDGREARYISNREALRLPNAKLAMIGFIFFS
                     ATESTTGLWCSSYLSAYHGFSAADAATVASCFYGGITVGRLISGFVSMKVKSPLLIRW
                     GQLICVAGTLLIILPLPAAFSVTGIVLTGLGTSPVFPGMIHETPNRFGKDVSQAIVGL
                     EMAVAYIGGTVSAPLFGQLASLVGIRLFPYYILACVLVMLIASEMLQKRIEDRDRQGQ
                     LAKIEG"
     gene            complement(2169..3023)
                     /locus_tag="PLG72_07695"
     CDS             complement(2169..3023)
                     /locus_tag="PLG72_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80718.1"
                     /translation="MKLEGLHEKEIFTERYPFRLVENTTDEFKYPMHWHNAAELVYNA
                     AGICCINVAGADYSLKDGELLLIPAGEIHEITSCSKSGKRYFIQFDISSLDGFGGIND
                     VRPFMEKITKIGDDTCPQLKVSVEKCVMELISEYEKKDYAYALSINARIFDIIVLVSR
                     SLAGSCPGAENTRSRTVAGLKQINKAFEYIERNYPLQITLKDISQAAGFSEYYFSRLF
                     KEYTGQSFLSYLNTYRVKQAAKLLKSTERSILEIAMDSGFNSLTTFNRIFRKIKGCSP
                     SYYRKAGI"
     gene            complement(3179..5353)
                     /locus_tag="PLG72_07700"
     CDS             complement(3179..5353)
                     /locus_tag="PLG72_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ80719.1"
                     /translation="METKPVYLDESLSFEERAKDLVSRMTLEEKVSQMLHRAPAIPRL
                     GIPAYNWWNEALHGVARAGVATVFPQAIGLAATFDEDLIYKIADIISTEGRAKYHENV
                     RKGDRDIYKGLTFWSPNINIFRDPRWGRGHETYGEDPYLTARLGVAFVKGIQGNDRKY
                     LKAAACAKHYAVHSGPESERHSFNAVVSKKDLRETYLPAFKALVKEAKVEAVMGAYNR
                     TNGEPCCGSKTLLTDILRNEWGFKGHVTSDCWAIKDFYEGHMVTKTAPESVALAVRSG
                     CDLNCGNLFINLLIAHKEGLITEEEIDRAVTRLFVTRMKLGMFDDPKNVPYSDTPYEV
                     NSCKEHSDFSVEVAKKTLVLLKNEGSLLPLDKKKLKSIAVIGPNADSRDALQGNYFGT
                     SDRYVTVLDGIRNAVGDDVRVYYSEGCHLYRDRSSNLAEPRDRFAEALACAERADVVI
                     MCLGLDATIEGEEGDTGNEFASGDKLNLELPGLQQELLEKVYAAGKPIVLVLLSGSAL
                     AVRWADEHIPAIVQAWYPGGRGGEAVASLLFGEYSPSARLPVTFYRTTEELPDFRDYS
                     MKNRTYRYMENEALYPFGYGLSYTKFEYSPIKAEKTVINAGEDFTCSIRVKNIGSMAS
                     EETVQLYLKDVEASVEVPRWSLNGIRKVYLEPGCEKEVTFTLTPRQMALIDYEGKAIL
                     EPGTFEVFIGGSQPDARSISLIGTPVQKIAFEVTGERMELEY"
     gene            5635..6459
                     /locus_tag="PLG72_07705"
     CDS             5635..6459
                     /locus_tag="PLG72_07705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HOJ80720.1"
                     /translation="MQIGNVGTFSIISMIISAALSILVPIALIIILGIRKKLNWKAML
                     LGSGLFLLFVMILERLMHWAVLGPDPAASALYRNKWLYMLYAGFAAGIFEETARLLGF
                     RFILRVSENESIDTGISYGLGHGGIESILIVGISTVTNISMAYMHNSGALNSMYAALP
                     EAERESLSEYIKLLVTTPPYEFLMSGFERVVALVLQISMSLFVLKAVSQKKWQYFLFA
                     ILLHAGVDIIAVHYSLGMITNLFLLEGILAVIAVTFAAVAFRTYHGRVKIQPENIQ"
     gene            6728..>6910
                     /locus_tag="PLG72_07710"
     CDS             6728..>6910
                     /locus_tag="PLG72_07710"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOJ80721.1"
                     /translation="MPIRIPDSLPAAEVLTNENIFVMTERRAESQDIRPLNIVVLNLM
                     PTKIVTETQLLRLIGNT"
BASE COUNT         1580 a         1753 c         1796 g         1781 t
ORIGIN      
        1 agaaaggaac gctgaaggat gtcatcgtcg gcgccgatgt gttcatcggc ctgtttgtgg
       61 cgggtgtgct gacaaaggat atggtcaggt cgatggcaaa agatccggtc atatttgcca
      121 tggcgaaccc gacgcctgaa ataatgccgg acgaagcaat agaagcgggt gccaaagtaa
      181 tcggcaccgg gcgctccgat ttcccgaacc agataaacaa tgtccttgca ttcccgggca
      241 tcttcagggg agcgctcgat gtcagggcaa gggatataaa tgacgagatg aagatcgccg
      301 ccgcgcaggc aatagcaggc ctgatttcag acgacgaact gaatcccgaa tatgtcatac
      361 ctgctccctt cgatcccagg gtgtcaaagg ctgtggccaa agctgtcgcc gatgcggcta
      421 taaggacagg agtcaatagg atcggcggca ggtaaaaccg gcagtatttg cagcaaacag
      481 acaaattact aaaggacata caggacaata tgcacaaaga tatattggaa gacatataag
      541 aaggatccgc aatgaacatt ttactggtga tcatatatct tgcctttatc agcctggggc
      601 tccctgattc gcttctcggg agcgcctggc ctgcaatgag ggcggacctt ggcgcgcctg
      661 tttcgacagc cggatggctc gccatgacga tcagtgtgtg tacgatcata tcaagcttat
      721 tctcaaacag ggtcataacc aggttcaaaa cagggaaggt gactgcggtc agcgtggcca
      781 tgactgcagt cgctttattt ggatttgcct ttgcgccgtc ggtgtggtcc atgttcctca
      841 tggccattcc gctggggctt ggagccggca gcatcgacgc tggactgaac aattacgtcg
      901 cgctccacta cgaatcaagc cacatgaact ggctccacag tttctgggga ataggcgcga
      961 ctgtcggacc gatgatcatg gcataccata tcgcggcgca gaatggctgg cgatatggct
     1021 accttaccat tgccgccgtc caggccgtac tggtcgccgt acttttcgct tcgctgaaac
     1081 tgtggaaaaa cggagccgac acaagcagtt ccgatgacgg gagagaggca agatacatct
     1141 caaacagaga agcgttaagg ctccccaatg caaaacttgc gatgatcggc tttatattct
     1201 tcagcgctac cgagtcgacc accggcctgt ggtgcagcag ctacctttcc gcataccacg
     1261 ggttttccgc cgccgacgct gcgacagtcg cttcctgttt ttacggcggg atcacggtcg
     1321 gcagacttat atcaggattt gtatcgatga aggtaaagag cccgctcctg ataagatggg
     1381 gccagctcat atgcgttgcg ggaactttgc ttatcatttt accgctgcct gccgcttttt
     1441 ctgtaacagg catcgtactg acgggcctgg ggacttctcc tgttttcccc ggcatgatcc
     1501 acgaaactcc aaacaggttt ggcaaggatg tgtcgcaggc gatcgtgggt cttgaaatgg
     1561 cggtggcata tataggaggt accgtctcag cgcctttgtt cggccagctt gcgtcgcttg
     1621 tcggtatcag gctgttcccg tactatatac tggcttgcgt cctggttatg ctgatcgcat
     1681 cggaaatgct gcaaaaaagg atagaggacc gggacaggca gggacaattg gctaaaatcg
     1741 aaggatagag gaagatacga aaaaatgaaa aatgaataag gggaaagcca tcagcggcct
     1801 tccccttcac tgtatctgct ttgttacctg tacccgagga ggaagcaaat acagctttat
     1861 acagagtcgc acgtaatgtg tttttgctgc tgcatacctg tggtacgctg aagtagtact
     1921 cggtgccgtc tgcaagctgt ctggaaacat tagctgttat cattgtcagt atggacactg
     1981 ctgcatatga tctgccgtga tgatcccaaa aagttttccg tacctgatac tcctcccaac
     2041 agacacaata cccgctatta atagtatggc ataactgttg tgccagttgg ttacaataca
     2101 gtgaatttta cttaacaaga atattacaat tttgtggaga gggcggcgag cggagtgcag
     2161 ggcccgggtc atatcccggc cttgcggtaa taggacggcg aacagccttt tattttcctg
     2221 aatatcctgt tgaacgtggt cagactgttg aagcctgaat ccatggcgat ctccaggatc
     2281 gacctttcag tggatttcag cagctttgcc gcctgtttca ccctgtaagt attcagatag
     2341 cttaggaagc tctggccggt atactccttg aaaagcctgg agaaatagta ttcactgaag
     2401 cctgctgcct gtgagatgtc tttcagcgtg atctgcaaag ggtagttccg ctcaatatat
     2461 tcgaaagctt tattgatctg cttcagcccg gctacagtcc tgctcctggt gttttcggca
     2521 cccggacagg aaccggccag gcttcttgaa acaagcacta ttatgtcaaa tatccttgcg
     2581 tttatggata atgcatatgc atagtccttt ttttcgtatt cgctgatgag ttccataacg
     2641 catttttcta ccgatacttt cagctgcggg caggtgtcgt cgcctatctt cgtgatcttt
     2701 tccataaacg gcctcacatc gttgatgccg ccgaaaccgt caagtgatga tatatcgaac
     2761 tggatgaagt accttttccc gcttttgctg caagaggtga tctcgtgtat ctctcctgcg
     2821 ggtatcagca gcagttcgcc atctttaagc gaataatccg cccctgccac attgatgcag
     2881 cagatgcccg cggcattata gacaagttcg gccgcattgt gccagtgcat cggatatttg
     2941 aactcgtccg tcgtattttc cacaaggcgg aaaggataac gctcggtgaa tatttccttt
     3001 tcatgaaggc cttccaattt cataagaaca cctcgatatc atattatcag atatccgggg
     3061 gtgtccgcaa tgagtcggaa ggcggccgcc ttcaggctta ctttagccct gttggtcggc
     3121 cctcgtaaga aacggggacg gttcccgagt cttgcgggaa ccgtccccgt tcaatgcatc
     3181 aatattccag ttccatacgt tcgccggtga cctcgaaggc gattttctgc accggtgtgc
     3241 cgatcagact gattgacctt gcatccggct ggctgccgcc tatgaaaacc tcgaaggtgc
     3301 cgggctccag gatcgctttg ccttcataat cgatgagagc catctgcctc ggtgtcagcg
     3361 tgaaggtcac ttccttctcg cagccgggct caaggtaaac cttcctgatc ccgttgagcg
     3421 accaccgagg cacttcgaca cttgcctcga catctttcag gtaaagctgt acggtctctt
     3481 cagatgccat ggagccgatg tttttgaccc tgatgctgca tgtgaaatcc tcgcctgcat
     3541 tgatgaccgt cttttcagcc tttatcgggc tgtattcaaa tttggtatag ctaaggccgt
     3601 agccgaacgg atacagtgcc tcgttttcca tatacctgta tgtcctgttc ttcatagagt
     3661 aatcgcggaa atcgggcaac tcctcggtag tcctgtagaa tgtcaccgga agcctggctg
     3721 acgggctgta ctcaccgaac agcagcgatg cgaccgcttc gcctccgcgg cctcccggat
     3781 accatgcctg gactattgcg ggtatatgtt cgtctgccca tctgaccgca agggcgcttc
     3841 ctgacagaag tacaaggacg atcggttttc cggcggcata caccttttcc agcagttcct
     3901 gctgcaggcc gggaagctcg agattcaact tgtcgccgct ggcgaattcg ttgcccgtat
     3961 cgccctcttc gccctcgatg gttgcatcca ggccgagaca catgatgaca acgtcggccc
     4021 tttccgcaca tgccagggct tcggcgaacc tgtcccgcgg ttctgcgaga ttggaactcc
     4081 tgtccctgta aaggtggcag ccttcagagt aatagacccg tacgtcgtcg cccactgcat
     4141 tcctgatgcc gtcaagcaca gtaacatacc tgtccgacgt cccgaaataa ttgccctgca
     4201 gtgcatccct gctgtctgcg ttgggaccta tgactgctat ggatttcagt ttctttttat
     4261 cgagaggcag caggctgcct tcgtttttca gcagtaccag cgtctttttc gcaacttcca
     4321 cggagaagtc gctgtgctcc ttgcagctgt tgacttcgta aggcgtgtcg gaatacggta
     4381 cgtttttggg atcgtcgaac atgccgagtt tcatgcgtgt tacaaacagc cttgttacgg
     4441 cgcggtctat ttcttcttcg gtgatcaggc cttccttgtg ggctatcagc aggttaatga
     4501 acaggttgcc gcaattgagg tcgcagccgc tcctgacagc cagtgcgaca gactcgggag
     4561 cagtcttcgt taccatatgg ccttcataga agtcctttat tgcccagcag tcggaagtga
     4621 catggccttt gaatccccat tcgttgcgga gtatgtcggt aagcagcgtt ttgctgccgc
     4681 agcagggctc gccgttggtc ctgttgtatg cgcccatgac ggcttcaact ttggcttctt
     4741 tgaccaaagc cttgaatgcg gggagatatg tctcgcgcag atcctttttt gatactaccg
     4801 cattgaaact gtgcctttcg ctttccggac cgctgtggac ggcgtaatgc ttagcgcagg
     4861 cggcggcctt cagatacttt ctgtcattgc cctggatgcc tttcacgaaa gccactccaa
     4921 gccttgcagt gaggtacgga tcttcgccgt aggtctcatg gccgcgtccc cacctgggat
     4981 ccctgaaaat atttatgttc ggcgaccaga aagtaaggcc tttatatatg tcgcggtcgc
     5041 ccttgcggac attctcatgg tacttggctc ttccttcggt ggaaatgatg tcggcgatct
     5101 tatagatcag gtcttcatca aaggtggcgg ccaatccgat ggcctgcggg aatacggtgg
     5161 ccacacctgc ccttgccact ccgtggagcg cttcgttcca ccagttgtaa gccggtattc
     5221 cgagcctcgg gatggcaggc gccctgtgca gcatttgtga aactttttct tcaagggtca
     5281 tgcgggatac gaggtccttc gcccgctctt caaagctgag gctttcatca aggtaaacgg
     5341 gtttggtttc cataacaagt tacctccttt gctttttatt catgcatatt tttcttccaa
     5401 taataccgat ttacatatat acttaattcg atactatatt tttttcggcg ttaaatcaaa
     5461 tcaaaacttg ctgaccattg ctcagttttt gcgcaacaga gcatgtgccg cgcggcggac
     5521 gcagcattca gcccgggcag gaaaaaggct catcagatgc gcaggcggac tgccggcctg
     5581 aaatctggta gtcgtacgta acagcgctat aataaacagt ttggaggtaa gtttatgcaa
     5641 atcggcaatg taggcacatt ttccatcata tctatgataa tatcggcagc attgtccatt
     5701 ttggtaccga tcgccctgat cataatctta ggaataagga aaaaattgaa ctggaaagcg
     5761 atgctgctgg gcagcgggtt gttccttctt ttcgtaatga tacttgaaag acttatgcac
     5821 tgggcagttc tggggccaga tcccgccgcg tctgcactgt atcggaataa atggctgtat
     5881 atgctgtatg ccgggtttgc ggccggtatt ttcgaagaga ctgccaggct gctcggattc
     5941 agattcatac tcagggtatc cgaaaacgaa agtatcgaca ccggcatatc gtacggactg
     6001 gggcacggcg gcatcgaatc gatattgatc gtcggcattt cgaccgtcac caacatttct
     6061 atggcgtaca tgcataactc cggtgccctg aacagtatgt acgctgcact gccggaagcg
     6121 gaacgggaat cattgagcga gtatataaaa ctgctggtga cgactccgcc atacgagttc
     6181 ctgatgtccg gattcgaaag ggtcgtggcg cttgtgctgc agatctccat gtcgctgttc
     6241 gtactcaagg ctgtttctca gaaaaagtgg cagtacttcc tgtttgcgat tcttttgcac
     6301 gccggcgttg acataatcgc cgtacactac agcctgggaa tgataaccaa cctcttcctg
     6361 cttgagggta tcctggcggt catagccgta acttttgccg ctgttgcatt caggacttac
     6421 cacggaaggg tgaaaataca gccggaaaac atacagtaaa gcgtatcggg gaagcacatc
     6481 ggaaattgtg acggaacaag gccgtgagca ggcggaacag cggcatctgc cctgatgtgc
     6541 aatgtcgcta ttgattattt ttctcccatg ctctattata tttaacatat cacccaaaat
     6601 ctgagcaaaa gaccgtccat atatgctgca acactgctga tggaagggga cccgggcgga
     6661 ccgttatctc cgggtcggag tgttaaccgg gcgggagccg aacgagtaca tgaaaggtgg
     6721 aaaatgaatg ccgattagga ttccggacag cttgccggca gcggaggtac tgaccaacga
     6781 aaatatattc gttatgacag agcgcagggc tgagagccag gatatcagac ccctgaatat
     6841 agttgttctc aacctgatgc cgacaaagat cgtaactgag actcagttgc tgaggcttat
     6901 tggaaacacg
//
LOCUS       DAOSCC010000131         6828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7150_length_6828_cov_4.384763, whole genome shotgun sequence.
ACCESSION   DAOSCC010000131 DAOSCC010000000
VERSION     DAOSCC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7150_length_6828_cov_4.384763"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1569)
                     /gene="gpmI"
                     /locus_tag="PLG72_07715"
     CDS             complement(37..1569)
                     /gene="gpmI"
                     /locus_tag="PLG72_07715"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ80722.1"
                     /translation="MKNKLVTLIILDGFGINERKEGNAVAAAYKPNLDRYMKEYPNTI
                     IHTSGLDVGLPKGQMGNSEVGHTNIGAGRIVYQELTRITKSIEDGDFFEKKELVDAME
                     NCRKNGTKLHLYGLVSDGGVHSHNTHLYALAEMAKKFGLKDVFVHCFFDGRDVPPDSA
                     KDYVAELEAKLKEIGVGKIASVMGRYYAMDRDNRWERVKLAYDAMVLGQGLTAESAAE
                     AVAASYGREEFDEFVKPTVILTDGKPTATIGQNDSIIFFNFRPDRAREITRAFVDPEF
                     KGFERAKGFFPVHYVCMTQYDKTMPNVTVAFKPESLTNTFGEYISKNGLRQLRIAETE
                     KYAHVTFFFNGGTETMYEGEDRCLIPSPKVPTYDMKPEMSAYEVTEEVLKRIDSRQYD
                     VIILNFANCDMVGHTGVFEAAKAAVEAIDKCVGRIVDAVRAQDGIVLITADHGNAEQM
                     IDYETGGPYTAHTTNVVPLIGIGIGGRQLKEGRLADLAPTMLELLGFEKPVEMTGESL
                     LR"
     gene            complement(1716..3656)
                     /gene="tpiA"
                     /locus_tag="PLG72_07720"
     CDS             complement(1716..3656)
                     /gene="tpiA"
                     /locus_tag="PLG72_07720"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF010569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ80723.1"
                     /translation="MALYNKKTIEDIDVRGKKVIVRVDFNVPLDENRNITDDKRIRAA
                     LPTIKYLVDNKAKTILVSHLGRPKEGPEEKYSMKPTAVRLGELLGQPVIMANDVIGPD
                     AKAKAAALKEGEVLMLENVRFHKEETKNDPAFAKELASLAEIYVNDAFGTAHRAHAST
                     AGLADYLPAVCGYLIQKEIEIMGKALSNPERPFVAILGGAKVSDKISVIENLIDKVDT
                     LIIGGGMAYTFLKAKGYKIGDSICEDDKLDLAKSLMEKAEQKGVKLLLPIGSIVGKEF
                     KPDTEYKYVPSDDMPDGWMGMDIGSQTIEYFSKEIRKAKTIVWNGPMGVFEFENFANG
                     TREIARAVAESGAVSIVGGGDSAAAIEQLGFADKITHISTGGGASLEFLEGKVLPGIA
                     VLMDKNPRKKVIAGNWKMNKTAKEAAEFVKALIPRVADVQADVVVGAPFVCLPDVVKA
                     AAGSNIKVAAQNMHWEEKGAFTGEVSGLMLKDLGVEYVIIGHSERRQYFAETDETVNR
                     KVHAALKYGLKPIVCVGESLTQREQGVTAELVSYQVKIALLGLTAEQVKDVIIAYEPI
                     WAIGTGKTATNEQANEVCIIIRNTIKSLYGEETAEAIRIQYGGSVTAANAEGLFSMSD
                     IDGGLVGGASLKLDDFEKIVKS"
     gene            complement(3788..4801)
                     /gene="gap"
                     /locus_tag="PLG72_07725"
     CDS             complement(3788..4801)
                     /gene="gap"
                     /locus_tag="PLG72_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ80724.1"
                     /translation="MAIKIGINGFGRIGRLVFRAAVDNPEVEVRGINDPFIDLDYMVY
                     MLKYDSVHGHFKGEVHTEGDKLVVNGKKISVYASMNPDEIPWSECGAEYIVESTGVFL
                     TTEKASAHFKGGAKKVVITAPAKDDTPMFVMGVNNDKYTKDMKIVSNASCTTNCLAPL
                     AKVIHDNFGIVEGLMTTVHSITATQKTVDGPSKKDWRGGRAAAHNIIPSSTGAAKAVG
                     KVIPELKGKLTGMSFRVPTLDVSVVDLTCRLEKPATYDEIKEAVKKASEGELKGILGY
                     TEDDVVSSDFIGDPRTSIFDAKAGISLNDHFVKLVSWYDNEWGYSNKVLDLIMHMAKV
                     DAQ"
     gene            complement(5077..5973)
                     /locus_tag="PLG72_07730"
     CDS             complement(5077..5973)
                     /locus_tag="PLG72_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOJ80725.1"
                     /translation="MEFSEITLNSKHLFDQYIKKHKPEISELTFTNFFAWRYYYRFRY
                     AVVSDLLCVIAAPAKKQPFAMMPIGDVDERNFEEAYNAIRSYFAERGWKLCFGRITRD
                     ELAYFRDKVADEASIVYDRDNSDYLYNTRDLAELKGKKYDGKRNHISRFMREHTFEYV
                     PLECGLLDECARIMEEWCRDKDCSCQDGDYCERNANLEILRHFKTLGCKGALIKVDGE
                     FAAFTVGEMLNEDTAVIHIEKARSSINGLYTVINQQFVQREWSSTAYINREQDLGLEG
                     MRKAKLSYHPVTLVEKFNVYPV"
     gene            complement(6039..>6828)
                     /locus_tag="PLG72_07735"
     CDS             complement(6039..>6828)
                     /locus_tag="PLG72_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ80726.1"
                     /translation="SGIVSILEALRVVKEKEIEHGDIQVVFTISEEGGLYGAKYLDYS
                     RIYAKYGFVMDGDGPIGTVSVKAPSQDKILVTVEGKASHAGVAPEKGVDAIRIAASAI
                     SGMKLGRIDYETTANVGVINGGRETNIVCDRVELKAEARSRDDRKLDDQTRHMRERFE
                     QAASEFGGKVDFRAERMYKSFNITKDAPIMGILERAAENAGISLIAEETGGGSDTNIF
                     NEKGIESVAVSSGMQKVHSTEECISINDMVKAAEFVVSIIRSVS"
BASE COUNT         1389 a         1925 c         1539 g         1975 t
ORIGIN      
        1 tccagcagag aagtgtcccc tttccttatt ctccccttac cttaacagcg actcgcccgt
       61 catttctacc ggcttctcga agcccagcag ttccagcatc gtcggtgcaa ggtccgccag
      121 gcggccttcc ttaagctgcc tgccgccgat gccgatgcct atcagcggca ccacgttggt
      181 ggtgtgggcc gtgtacgggc cgccggtctc gtagtcgatc atctgctcgg catttccatg
      241 gtccgcggtt ataaggacga taccatcctg ggccctgaca gcatcgacta tccttccgac
      301 gcatttgtcg atggcttcca ctgccgcctt tgcagcctca aataccccgg tatggcctac
      361 catgtcgcag tttgcgaagt tcagtatgat cacgtcatac tgccttgagt cgatcctctt
      421 cagtacttct tccgtaacct cgtatgcgct catctcaggc ttcatgtcgt atgtgggcac
      481 cttcggcgac ggtatcaggc acctgtcctc gccttcatac atcgtttcgg tcccgccgtt
      541 gaagaagaac gtgacatggg catacttctc ggtttcagct atcctgagct gcctgagccc
      601 atttttgctg atatattcac cgaatgtgtt ggtcagcgac tccggcttga aagccactgt
      661 tacgttcggc atggttttgt catactgggt catgcagaca tagtggacag ggaagaaccc
      721 tttggccctt tcaaaacctt tgaattcagg gtccacaaat gccctggtta tttctcttgc
      781 cctgtcgggc ctgaaattga agaatattat cgagtcattc tggcctatgg tggcagtagg
      841 tttgccgtct gtcaggatga ccgtgggctt gacgaattcg tcgaactcct cgcgtccata
      901 tgatgccgca acagcctccg ctgccgactc agctgtcagg ccctggccaa gcaccatcgc
      961 gtcatatgcc agcttgaccc tttcccagcg gttgtccctg tccatggcat aatacctgcc
     1021 catcacgctg gctatctttc ctacgccgat ctccttaagt ttggcttcga gttccgcaac
     1081 ataatcctta gcgctgtccg gcggcacgtc cctgccgtcg aagaagcagt gtacgaatac
     1141 gtccttcagt ccgaactttt tagccatctc ggccagcgca tacaggtggg tgttgtggct
     1201 gtgtacgcct ccgtcggaca ccagtccgta caggtgcagc ttcgtgccgt tcttcctgca
     1261 gttctccatg gcgtcgacga gttctttctt ttcaaagaag tcgccgtcct cgatggactt
     1321 ggtgatcctt gtgagttcct ggtaaactat cctgccggcg ccgatgttcg tatgtccgac
     1381 ctcggagttg cccatctgtc cttttggaag gccaacatcc aaaccgctcg tatgtatgat
     1441 ggtgttcgga tattccttca tgtaacggtc caggttaggc ttgtatgccg ccgctacagc
     1501 gttgccttct tttcgttcgt tgatcccgaa tccgtccagt atgatcaatg ttaccaattt
     1561 atttttcata tatgatccac tccttgtgtt acggcctgta taaaatctca aaaaggctgt
     1621 cctttcagac agccttttga tcgtccattg ccgggtacac tcctgtaaag ccgtttaccg
     1681 gatcactgtc cggaaggtta actgtccctg gtccattatg acttgactat cttttcgaag
     1741 tcgtcaagct tgaggcttgc accgccaaca aggccgccgt cgatgtccga catgctgaag
     1801 aggccttccg cattggcagc agtgacgctt ccgccatact ggatgcggat cgcttcagca
     1861 gtctcttctc catagaggct ctttatggtg ttcctgatga ttatgcaaac ctcatttgcc
     1921 tgctcattcg tcgccgtctt cccggtaccg attgcccaga tgggctcata agctattata
     1981 acgtctttga cctgttcggc cgtgagcccg aggagagcga tcttgacctg gtagcttaca
     2041 agctcggctg tgactccctg ctccctctgt gtcagcgact caccgacaca aacgatgggt
     2101 ttgagtccgt atttgagtgc tgcatggacc ttcctgttga ctgtctcgtc ggtttcggca
     2161 aaatactgtc tcctctcgga atggcctatg atgacatatt ccacgccaag atccttaagc
     2221 atcaggcccg aaacctcacc ggtgaatgct cctttttctt cccagtgcat gttctgagct
     2281 gcgaccttga tattcgatcc tgcagcggct ttaacaacat ccggcagaca tacgaatggc
     2341 gcgccgacaa ctacgtctgc ctgaacatct gctactctcg gtatcagagc cttaacgaac
     2401 tctgctgcct ctttcgcagt tttattcatt ttccagttgc cggctatcac tttttttcgc
     2461 gggttcttat ccatcaggac tgcaattccg ggaagtactt ttccttcgag gaattccagc
     2521 gatgctcctc cgcctgttga gatatgggtg atcttatcgg caaagccaag ctgttcgatc
     2581 gcagctgcgg aatcgccgcc gccgacaatc gaaacggctc cggactcggc gacagccctt
     2641 gctatctccc tggtaccgtt tgcgaagttc tcgaactcga atacgcccat gggaccgttc
     2701 catacgatgg tctttgcctt tctgatctct ttcgagaagt attcgatggt ctgcgagcct
     2761 atatccatgc ccatccagcc gtcaggcata tcgtcggaag gtacgtactt gtactcggtg
     2821 tccggtttga attccttacc gactatgctg ccaatcggga gcaacagctt gactcccttc
     2881 tgctcggctt tttccatcag gctctttgcc agatccagct tgtcgtcctc gcaaatggaa
     2941 tcgcctatct tgtaaccctt tgccttgagg aatgtataag ccattccgcc gccgatgatc
     3001 agcgtatcaa ctttatcgat aaggttctct attacgctta tcttgtcgga tactttcgca
     3061 ccgccaagta tggccacgaa aggcctttca ggattggaga gggctttgcc cataatttcg
     3121 atttccttct ggatcaggta tccgcatacg gccgggagat aatcggccag gcccgccgtg
     3181 gaggcatgtg ccctgtgtgc ggtaccaaaa gcgtcgttta catagatttc tgcaagtgat
     3241 gcaagttcct ttgcaaatgc aggatcgttc tttgtctcct ctttatggaa cctgacattc
     3301 tccagcatca gcacttctcc ttccttcagc gctgctgcct tcgcctttgc gtcaggccct
     3361 atgacatcgt ttgccatgat gaccggctgt ccaagcaatt cgccaagcct tacggctgtc
     3421 ggcttcatgc tgtatttctc ttccggtcct tctttcggcc ttccgagatg ggaaaccagt
     3481 atagtctttg ctttattgtc gacaaggtat ttaatggtcg gaagtgccgc cctgatcctc
     3541 ttgtcatcgg ttatgttgcg attttcgtcc agaggtacgt tgaagtcgac cctgacgatg
     3601 acttttttgc ctcttacgtc tatgtcttcg atcgtcttct tattgtacaa tgccatacaa
     3661 tccacccttt cgaacttcga ttcgcggctg tttctccgca tccgtcataa tcaaaaggcc
     3721 caccctttga ccactcactt aaggtaaggg caagagcggg ccctgatcag tccttaacga
     3781 tcaagcatta ttgtgcatcg actttcgcca tgtgcatgat gagatcgagg accttgttcg
     3841 aatagcccca ctcattgtca taccatgaaa cgagctttac aaagtggtca ttgagagaga
     3901 ttcctgcttt tgcatcgaag atcgatgtac ggggatctcc gatgaagtcc gatgaaacga
     3961 cgtcgtcttc ggtatatccg aggataccct tgagttcgcc ctcggaagcc ttcttgacag
     4021 cttccttgat ctcatcgtat gttgcaggct tctcgagacg gcatgtgagg tcgactaccg
     4081 atacatcgag ggtcgggacc ctgaacgaca tgcctgtcag cttgcctttc agttcgggga
     4141 tgacctttcc gactgccttt gcagcgccgg tcgaagaagg aatgatgttg tgtgcagcag
     4201 cacggccgcc tctccagtct ttctttgaag gaccgtcaac tgtcttctgg gttgcggtga
     4261 tcgagtgaac tgtcgtcatt agaccttcaa ctatgccgaa gttgtcatgg atgacctttg
     4321 caagaggcgc cagacagttc gtggtgcagg atgcgttgga tactatcttc atatccttcg
     4381 tgtacttgtc gttgtttacg cccataacga acatcggggt gtcgtccttt gccggcgcgg
     4441 tgatgacgac tttctttgcg ccgcccttga agtgagccga tgctttttca gttgtcagga
     4501 acacgcctgt agactcaact atgtattcag caccgcattc gctccagggg atctcgtcgg
     4561 gattcatgga cgcgtagacc gagatcttct tgccgttaac gaccagcttg tcgccctcgg
     4621 tatggacctc gcccttgaaa tggccatgga ccgaatcata cttgagcata tataccatgt
     4681 aatcgagatc gatgaacggg tcatttatcc ctcttacctc aacttcagga ttgtcgaccg
     4741 cagctctgaa tacgagacgt ccgattcttc cgaaaccgtt gatacctatt tttattgcca
     4801 tttcctctta cctcctatat aaattatggt tattactttc caaaatcaca actattttat
     4861 attatttgat atagtttttc aataccttga gccaaaaata attaaatttt taacaaagtc
     4921 gtgatgccat gttttcaaca gttccgcagg atgcctgcaa tcaacaatat gttctcctgt
     4981 atcgtacgaa agtatacgcc cgaagtatac gcccgcagaa tggccgtggg tccggtgcca
     5041 aacttccgcc cgttactgca gccaggctga cgccgttcat acaggatata cgttgaactt
     5101 ttcgacaagc gtcacggggt gatatgaaag cttggctttc ctcatccctt caaggcccag
     5161 atcctgttcc ctgtttatat atgcagtgct gctccactca cgctgtacaa actgctggtt
     5221 tatgacagta taaaggccgt ttatagagct tctggccttt tctatatgga tcacggcggt
     5281 atcttcattg agcatctcgc ccaccgtgaa cgccgcgaac tcaccgtcca ctttgatcaa
     5341 cgcacccttg cagcccaaag ttttgaaatg cctaagtatc tcaaggttgg cattgcgttc
     5401 gcagtaatcg ccgtcctggc agctgcagtc cttatccctg caccattcct ccatgatcct
     5461 ggcacattcg tccagcagtc cgcactcaag aggcacgtat tcaaaagtgt gctccctcat
     5521 gaacctgctg atatggtttc gcttgccgtc gtatttcttc cccttcagct cggccaggtc
     5581 cctggtatta tacaggtaat cgctgttgtc cctgtcataa acgatggagg cttcatctgc
     5641 caccttgtcc ctgaaatatg ccagttcgtc ccgggtgatc ctgccgaaac atagtttcca
     5701 gcccctttct gcaaaatagc tcctgatcgc gttatatgct tcttcgaaat tcctttcgtc
     5761 gacatctcca atgggcatca tcgcaaaagg ctgttttttg gccggagccg ctatgacaca
     5821 cagaaggtct gatacgaccg cgtacctgaa cctgtagtaa tatctccacg caaaaaagtt
     5881 ggtaaacgtc agttcggata tctcaggctt atgtttcttt atatattggt cgaacagatg
     5941 cttgctgttc agcgttattt ccgaaaactc catatttaaa tgcactccag tcattgttcc
     6001 cggtcattgt ataaagtcaa tataccaaca ttcgccgttt acgaaacact gcgtattatg
     6061 gaaaccacaa attcggcggc ttttaccatg tcatttatcg atatgcattc ctcggtgctg
     6121 tgcaccttct gcatccctga actgacagcc acagattcga tacctttctc attgaaaatg
     6181 ttcgtatcgc ttccgccgcc cgtttcctca gcgatcagac ttataccggc attttcagcc
     6241 gccctttcaa ggatacccat gatcggggcg tctttagtta tattgaagga tttgtacatc
     6301 ctctccgccc tgaagtcaac cttacctccg aattcggatg ccgcctgctc gaaccgttcc
     6361 ctcatgtgcc gtgtctggtc atcgagcttc ctgtcgtcgc ggctcctggc ctctgccttc
     6421 agttcaaccc tgtcacagac tatgttggtt tcacggccgc catttatcac acctacattg
     6481 gcagtggtct cataatcgat cctgccgagt ttcatccctg aaatggcgga tgcagcgatc
     6541 cggatagcat ctacgccctt ttcaggagcg acccccgcat gtgatgcttt tccttccacc
     6601 gttaccagta tcttgtcctg tgacggcgcc ttaactgata cagtgcctat cggcccgtcg
     6661 ccgtccataa cgaaaccgta ttttgcatat atcctgctgt agtccaggta cttcgccccg
     6721 tacagtccgc cttcctcgga aatcgtgaaa accacctgta tatcgccgtg ttcgatttct
     6781 ttttctttta cgactctgag ggcttccagt atgcttacta tcccggac
//
LOCUS       DAOSCC010000132         6806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7178_length_6806_cov_4.798548, whole genome shotgun sequence.
ACCESSION   DAOSCC010000132 DAOSCC010000000
VERSION     DAOSCC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_6806_cov_4.798548"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="ruvA"
                     /locus_tag="PLG72_07740"
     CDS             <1..605
                     /gene="ruvA"
                     /locus_tag="PLG72_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOJ80727.1"
                     /translation="AYIKGKLESRGSDYIIIEAGGVGYLIYTSQTTLQEAGRLEPGQE
                     FKAYTYLHVREDAMDLFGFATNEELSVFKLLLTVTGVGPKVALSLLSAVSPSKFSLAV
                     ITDDVRTLTKAPGVGNKMAQRIILELKDKIKKEQLDLKAAGPDLTLEPLVGSRLAEAV
                     SALMVLGYSQAEASQAVASVYSDELGLEEIIRNALKGLVR"
     gene            687..1718
                     /gene="ruvB"
                     /locus_tag="PLG72_07745"
     CDS             687..1718
                     /gene="ruvB"
                     /locus_tag="PLG72_07745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ80728.1"
                     /translation="MDEERLIGTKAVSEDIDETSLRPRRLDEYIGQKKVKENMKVFIE
                     AAKRRKEPLDHVLLYGPPGLGKTTLAGIIAAELGVNIRITSGPAIERPGDLAAILTNL
                     NEFDVLFIDEIHRLNRTVEEILYPAMEDFALDIIIGKGPSARSIRLDLPKFTLVGATT
                     RAGLLTSPLRDRFGVMNRLELYDVEELTLIVKRSAAILGIEIDDMGANEIARRSRGTP
                     RIANRLLKRVRDFAQVKGEGVIDAETAAMALNALEVDSIGLDSTDRNMLASIIEKFGG
                     GPVGLETLAACIGEEPDTIEDVYEPYLLQIGFIKKTPRGRMTTKLAYDHLGIRMKGGF
                     YEQQEMEID"
     gene            1871..3559
                     /locus_tag="PLG72_07750"
     CDS             1871..3559
                     /locus_tag="PLG72_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ80729.1"
                     /translation="MKKFTSFFLIISVVFLAGLSAVYAENGDGSAPDGGAVSSETVPA
                     DNRGRLEGITYSSDGVYEAADIAVTGFAYYYITELTDPLRIVIDMKNVTVPDEQGIIQ
                     ADGEYVKSIRYSQFTKRIARVVLDVAEGYDYSIVRTDNGLTAYVYKYGTPDQLREGST
                     IPLGPEYRISVSGSGADETVTIELGGYDEYSISRLTDPERLIVTIPEAYVSSKAKQFD
                     FNGKRISSVGYAPAGKSGAEITISLKAQLQYKTEVSEGKLNISFSLPSYKNITYSNSG
                     DRVYFLIEKAALTKGTKDLKPLYTDFVDESGRAYTVTFPSKNADLNEGILDINDCYLE
                     SFEVKNNGDGTTSLVFTGRPGNSYLVYTRDSGDTAVTVIRPAAGGQRIVAIDAGHGGT
                     ATGARYGDLYEKDLNLDIARRLESLLKSKGVQTCMIRSDDTNVDNYERAYLANKLGAA
                     LYLSIHNNAAGSGSVKGTMTLCYPTKKSGFTGRQFARIIQEQMVAALKTNDLNVRLRP
                     DLIVLRETSMPAALAEVAFMTNKQDRESLRSESFRQTAAGALCSSVMEALDRMA"
     gene            3803..5116
                     /locus_tag="PLG72_07755"
     CDS             3803..5116
                     /locus_tag="PLG72_07755"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ80730.1"
                     /translation="MRRFLVFFLVIQITVLLLAGCSIPFFGRSSDKPDKTGGVNDMIG
                     LDDSTGSNGTAAADAGMSDTDYIDPDSDGISEEDVSLNDPGTLTGDAAGSIFTSEPED
                     REEHYSNAEIGSGISEDTGNDGTGPYGTGVLAAQSVTKTAWSEPRRPVTVYYQDEDGC
                     MVPMTRWIQPQLGIARAAVSLAVDSPLTREETAYYGVYPVIPEGTEILGIDIRNGTAV
                     IDFSRDLLNYGTAWSERNIIAAVVYTLTEFDTVDKVRILINGYPVGVLKYGTDLSEPL
                     GREDLIINTEMVKALPGPGKEKVDVYFMKRANEDFVYAVPVSLAVESGEADLEPDTLV
                     RLLLGTVPGGGIYTEIPEGAYLRGSYVRDGTAYLDFSGEFLNYGGTAREEAILKQITY
                     TLRQCDGIRKIKITVDGSSTELPEGTYISAGLVIPATVNDVMDRG"
     gene            5124..5753
                     /locus_tag="PLG72_07760"
     CDS             5124..5753
                     /locus_tag="PLG72_07760"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOJ80731.1"
                     /translation="MDGGKQVPGKKRIIVATKNRGKLEEIAQLLSGLPYEVVSMTDAG
                     VTGDITEDGSTFEENALIKARTVWNATGDTVMADDSGLEVDYLGGAPGIYSARYAGQS
                     ATDEDKFCKLLKELSGVPEDQRNARFVCAIALILPDGREVTVRGTCEGYIAEEPAGAG
                     GFGYDPVFYVPEFGMTFAQMGEELKNSISHRGNALRKVLDILTEESKKH"
     gene            5815..6294
                     /locus_tag="PLG72_07765"
     CDS             5815..6294
                     /locus_tag="PLG72_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ80732.1"
                     /translation="MKILVISDTHGDTKKAEEAIRKNRGTDMVIHLGDYFRDAEKLSG
                     MFPGLPFEYIYGNSDFFANGIPAEKLLEVCGKKIFITHGHRYSAKWGYDKLFRKAEEL
                     KTDILMFGHTHMPDIVRRDGYTILNPGSTSDPRGDSMESYAIVEIQDGKVSARICRI"
     gene            complement(6468..6686)
                     /locus_tag="PLG72_07770"
     CDS             complement(6468..6686)
                     /locus_tag="PLG72_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ80733.1"
                     /translation="MPFYDLKCRCGEVFNIMASMSDRENKRIRCPKCGSNELEAVFTN
                     VNIIQTKGPAKAECPNFRQCGGMCRHLQ"
BASE COUNT         1838 a         1552 c         1951 g         1465 t
ORIGIN      
        1 acgcatatat aaaaggaaag cttgaatcca gaggcagcga ttacataatc attgaagcag
       61 gcggcgtggg gtatctgatc tacacttcgc agaccacgct ccaggaggcc ggccgcctgg
      121 aaccgggcca ggagttcaaa gcgtatacgt acctgcatgt cagggaagat gcgatggacc
      181 ttttcgggtt cgccacgaat gaggaactgt cggtattcaa gctgctgctc accgttaccg
      241 gcgtagggcc gaaagtggct ctgtcactgt tgtctgccgt gtcaccatcc aaattcagcc
      301 ttgccgtcat caccgacgac gtcaggacat tgacgaaagc tccgggcgtc gggaacaaaa
      361 tggcccagag gatcatcctg gaactcaagg ataagataaa gaaggaacag ttggatctca
      421 aggcggcagg accggatctg acgctggaac ccctggtggg ctcaaggctg gctgaagcgg
      481 tcagcgcgct tatggtgctg ggttactcac aggcggaggc aagccaggcg gtagcgtccg
      541 tttactcgga tgagctggga ctcgaggaga tcatcaggaa tgccctgaaa gggctcgtga
      601 gataaattcc tgggagacgg gtttcgttcc cggggccgaa caacgggcct gccgggaaag
      661 gataacggat ttgcggagga agaacgatgg atgaagaaag attgatcggg acaaaagcgg
      721 taagcgagga tatcgatgag accagtctca ggccgagaag gctggatgag tatataggac
      781 agaaaaaagt caaggaaaac atgaaggtgt tcatagaggc ggcaaaaaga cgaaaggagc
      841 ctttggacca cgtgctgctc tatgggcctc ccggcctcgg caagaccacg ctggccggca
      901 tcatagccgc ggaactcggc gtcaacatcc gcattacgtc cggacccgcc atagaaaggc
      961 ctggtgatct tgctgccata ctgaccaacc tgaatgaatt cgatgtgctt ttcattgacg
     1021 agatacaccg tctcaacagg acagttgaag agatacttta tcctgcgatg gaggatttcg
     1081 cactggacat catcataggc aaagggccga gcgcccgttc catcaggctg gatctgccga
     1141 aattcacgct ggtgggagcc accacgaggg cagggctgct gacatcgccc ctgagggaca
     1201 ggttcggtgt catgaaccgc cttgaattgt atgatgttga ggaacttact ctcatagtaa
     1261 agcgctccgc agcgatattg ggcatagaga tcgatgacat gggggcaaac gaaatcgcca
     1321 gaaggtcgag aggaacgccc aggatcgcca acaggctgct gaagagggta cgcgatttcg
     1381 cgcaggtcaa gggagagggc gtcatagatg ccgagactgc ggcaatggcg ctgaatgccc
     1441 ttgaagtgga ctccatcgga cttgacagca cggacaggaa catgcttgcc agcataatcg
     1501 agaagttcgg cggcggcccg gtaggactgg agacgcttgc cgcctgcatc ggcgaggaac
     1561 cggatacaat agaggacgtg tacgagccgt acctgctcca gatcggattc atcaaaaaaa
     1621 cgccgagggg caggatgacc acgaaacttg cctacgatca tctcggcatc aggatgaagg
     1681 gcggttttta cgaacagcag gagatggaaa ttgactgaga ttggcaatat tacagcatcg
     1741 ttaaattatc gttaagtaat ttggcaaatc aaaccccttg tcgaatttta tggtattctg
     1801 taagtaagtc aaatccttac gggcagctgt cttgcaggat cacaaaagaa aattcggcga
     1861 gggatctttt atgaaaaaat tcacttcatt ttttcttatt atttctgttg tctttttggc
     1921 cggattatct gccgtttatg cagaaaacgg cgatggatcg gcaccggacg gcggagctgt
     1981 gtcgtctgaa actgtaccgg ctgataacag gggccgcctg gaaggtatca catattcctc
     2041 tgacggggta tatgaagcag cagacattgc tgtgaccggt tttgcgtatt attacataac
     2101 ggaacttaca gacccgctga ggatagtgat cgacatgaaa aacgttacgg tcccggatga
     2161 acagggcatt atccaggctg acggcgaata tgtaaaaagc atcaggtatt cgcaattcac
     2221 taagaggatt gcaagggtcg tcctggatgt cgctgaaggt tacgactatt cgatagtgag
     2281 gaccgataac ggacttactg cgtatgttta caaatacggg actcctgacc aactccggga
     2341 aggcagcacg ataccgcttg gcccggaata caggataagc gtgtcgggaa gcggcgctga
     2401 cgagaccgtg acgatagagc ttggagggta cgatgaatac agcatatcaa gactgacaga
     2461 ccctgaaagg cttatcgtca ccatacctga agcgtatgtg tcttcaaagg cgaaacagtt
     2521 tgattttaac ggcaagcgga tcagttcggt cggatatgct cctgcgggaa agtcgggagc
     2581 agagatcacc atcagcctga aggcgcaact acagtataaa acggaagtat cggaaggaaa
     2641 acttaatatt tcgttctcat tgccatcata caaaaacatc acatacagca acagcggaga
     2701 cagggtgtat tttctgattg aaaaggctgc cctgacaaaa ggcacgaaag atctgaagcc
     2761 cctttatacg gatttcgtcg atgagagcgg cagggcctat acggtcacgt tcccgtccaa
     2821 aaatgcagac ctgaacgagg gtatcctgga cataaacgac tgttacctgg aatcattcga
     2881 agtgaaaaac aacggcgacg gcactacctc cctggtattc accggacggc cgggaaactc
     2941 gtatctggtc tataccaggg attcaggcga tactgcggtc actgtcatca ggcctgcagc
     3001 gggcggccaa aggatcgtcg cgatcgatgc ggggcatgga ggcacagcta ccggggcccg
     3061 ttacggggat ctgtatgaga aagacctgaa tctcgatata gccaggcgcc tggaatcgct
     3121 tctgaaaagt aaaggcgtac agacatgcat gatcaggtcg gatgatacca acgtggataa
     3181 ttacgagagg gcatacctgg ccaataagct tggagcggcg ctttatctaa gcatacacaa
     3241 caatgcggca ggctcaggca gtgtgaaagg gacgatgacc ctctgctatc ccacgaagaa
     3301 aagcggtttc accggcaggc agtttgcccg gataatacag gaacaaatgg tggcggcact
     3361 gaagacgaac gatctgaatg tgaggctgag accggacctt atagtgctga gggaaaccag
     3421 tatgccggcg gctcttgcag aggtagcgtt catgaccaac aagcaggaca gggagagcct
     3481 gcgcagcgaa tcgttcaggc agacggccgc cggagctttg tgcagttccg taatggaagc
     3541 cctggacagg atggcatgat gcggctggtt atcgtaatgt tgaaaactat gataatgttg
     3601 taaattatga ttaaaaaaat cagcagaatt tatgaattct gttgattttt tttgagtatt
     3661 ccgtgtggta acattatgta agattttaca cggccgccgt agctggaaat cacgcaggaa
     3721 gggcaatcat atccgggaaa tactaaaact tcctttagtt gtccataatc tccatgtaaa
     3781 ccaattatca tggagggacg atatgagaag gtttttggta tttttccttg ttatacagat
     3841 cacggtattg ctgctggcgg gatgctcgat accgtttttt ggcaggtcat ccgacaaacc
     3901 ggacaaaacc ggcggcgtca atgatatgat cggactggac gacagtaccg gcagcaacgg
     3961 gaccgcggct gcggatgccg gcatgtccga cacggattac atagatccgg attcggacgg
     4021 cataagtgaa gaggatgtca gcctcaacga tccgggtacc ctgacaggag atgctgccgg
     4081 ttccattttt acgtcggagc cggaagatcg tgaggaacac tattccaatg ccgaaatcgg
     4141 cagcggcata tctgaagata ccgggaacga tggtacaggc ccgtacggca ccggagtcct
     4201 cgccgcccaa agcgtcacga aaacggcctg gtctgagccg cggaggcctg tgactgtata
     4261 ttaccaggat gaggacggct gcatggtgcc catgaccagg tggatccagc cgcagcttgg
     4321 gatagccagg gctgccgtga gccttgccgt cgacagcccg ctgacgcgtg aggagacagc
     4381 gtattacgga gtgtaccctg taataccgga gggtaccgag atattgggta tcgatatcag
     4441 gaacggcacc gcagtgatcg acttcagcag ggatctgctc aactacggca ctgcctggtc
     4501 cgagaggaac ataatagctg ccgtggtata tacgcttacc gagtttgata cggtagacaa
     4561 ggtaaggata ttgataaacg gctatccggt gggtgtgctc aaatatggta cggatctgtc
     4621 ggaaccgctc ggaagagagg atcttataat aaatacggag atggtaaagg cattacccgg
     4681 gccgggaaaa gaaaaggtcg acgtgtattt tatgaaacgg gccaacgagg actttgtcta
     4741 tgccgtaccg gtatcgctcg ctgttgaaag cggagaagca gacctggagc ccgatacgct
     4801 tgtcaggctg ctgcttggga ctgtgcccgg agggggtata tataccgaga tacctgaagg
     4861 agcttatctt agaggcagtt atgtacgcga cggcaccgcg tacctcgatt tcagcggcga
     4921 gttcctgaac tacggcggaa cggcgagaga agaggcgatt ttaaaacaga taacgtacac
     4981 gctgcggcag tgtgatggca tcagaaaaat aaagatcacg gtggacggga gcagcaccga
     5041 actgccggaa ggcacatata tctcggcagg ccttgtgatc ccggcaactg tgaatgatgt
     5101 aatggacagg ggttagaatg caaatggacg ggggaaaaca ggttcctggg aaaaagagga
     5161 tcattgtggc aacaaaaaac agggggaagc tcgaggaaat cgcgcagctg ctttccgggc
     5221 ttccctatga agttgtttcg atgacagacg ccggggtgac cggcgatata acggaggacg
     5281 ggtccacatt cgaagaaaat gcgcttataa aggcaaggac agtctggaat gcgacgggcg
     5341 ataccgttat ggctgacgat tcaggcctcg aagtcgatta cctgggcgga gcgccgggta
     5401 tatattcggc aagatacgcc ggacagtctg caaccgatga ggacaaattc tgcaaactcc
     5461 tgaaagagct ttccggcgtt ccggaagacc aacggaacgc caggttcgta tgcgccatag
     5521 cgctcatcct gcctgacgga agggaagtca cggtaagagg tacatgcgaa ggttatattg
     5581 ccgaagagcc ggcgggagcg ggcggatttg gatatgatcc cgttttctat gttccggaat
     5641 tcgggatgac attcgcacaa atgggcgaag aattgaaaaa cagcataagc caccgtggca
     5701 atgccctgag gaaagtactg gacatcctga ctgaagaatc gaaaaaacat tgacaggcaa
     5761 ggcgtcagaa atcggcatcc tatgtcacag catggttaac gggggttttt cataatgaag
     5821 atattggtca taagcgacac gcacggggac acaaaaaagg ctgaggaggc tatccggaaa
     5881 aacaggggaa cggatatggt catccatctc ggagattatt tcagggatgc ggagaaattg
     5941 tccggtatgt ttccgggact tcctttcgaa tatatatacg gcaacagcga ttttttcgca
     6001 aacggcatac ccgcggaaaa actgctcgag gtctgcggaa agaagatctt tataacgcac
     6061 gggcacaggt attctgccaa gtggggctat gataagctct tcaggaaggc ggaggaactc
     6121 aagaccgaca ttttgatgtt cggccatacc catatgcccg acattgtaag aagagacggg
     6181 tacacgattc tcaaccccgg aagtacgagc gatccaaggg gggattccat ggaatcgtat
     6241 gccattgtgg agatccagga cggcaaagta tcggcaagga tctgcaggat atgacccgcg
     6301 cgccgcacat catcgcgtat ccggcgccag gatcgttgag ggtcagtata gttcctgcct
     6361 caatccgtca cactgtatcc ctggaaaagt ataagtgtgt tctttttcag catgatgaaa
     6421 ctctccttgt gctcagctgt catcggctgc gataccggac ttcaggatta ttgcaggtga
     6481 cggcacattc cgccgcattg ccggaagtta ggacattccg ctttggccgg tcctttcgtc
     6541 tgtatgatat tgacattggt aaatactgct tcaagttcat tgctgccgca ttttggacat
     6601 ctgatgcgct tgttttccct gtcgctcatg gatgccatta tgttgaaaac ttcgccgcat
     6661 ctgcacttca ggtcataaaa cggcaatacg tccacctccg ctgagaaaat gctgtcatct
     6721 tacagtttac catattgcgc cgaccgtgat aagcattatt gttaaaacat gaattatatg
     6781 ttatcatttt ttcagaaatc ccacat
//
LOCUS       DAOSCC010000133         6788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7215_length_6788_cov_4.678598, whole genome shotgun sequence.
ACCESSION   DAOSCC010000133 DAOSCC010000000
VERSION     DAOSCC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7215_length_6788_cov_4.678598"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..598
                     /locus_tag="PLG72_07775"
                     /pseudo
     CDS             1..598
                     /locus_tag="PLG72_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
     gene            1226..1597
                     /locus_tag="PLG72_07780"
     CDS             1226..1597
                     /locus_tag="PLG72_07780"
                     /inference="COORDINATES: protein motif:HMM:NF028436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HOJ80734.1"
                     /translation="MNWFPDGVFLLKGRHLYHLPEPPPLLSGLKVVKFGWFLGTVEDN
                     RFEPSHSLVMALDKGAFKRTLDLGTESAEVRSYLKGETLILKGDKGLTAVCVDGKTLG
                     WAKQTGDMLKNMYPKGWRMMK"
     gene            1691..2437
                     /locus_tag="PLG72_07785"
     CDS             1691..2437
                     /locus_tag="PLG72_07785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ80735.1"
                     /translation="MKNTVRLDKLLANFGFGSRNDIKKMVRNGMVTVDGEVARDSSMH
                     VDPEKSVISANGMTLNYRKFIYVMMNKPTGVVSATYDARLRTVTDLLPDELACFNLFP
                     AGRLDIDTEGLVLLTNDGQLAHNLLSPKKHVAKRYYALVAGQVTENDVAAFSEGVVLD
                     DGYKTMPAELIIIRSGLRSEIELVLHEGKFHQVKRMFEAVGKKVLYLKRIQIGGLKLD
                     ETLEPGECRELTPDELEQLKSGDTSHLFDS"
     gene            complement(2928..3878)
                     /locus_tag="PLG72_07790"
     CDS             complement(2928..3878)
                     /locus_tag="PLG72_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ80736.1"
                     /translation="MGNKTGKAEKSSKKIMTPELLVSRYRKRFRVTLGVLIGFILATG
                     VFVFFNYDYLAFKIFITSSYIYTETLDDLYKKYLDVDTKGRYYNNFDNFVIAAVTERI
                     RAERGDRYTYLYTPSSLERSRQLDKEEAAQSEITFPDDRTVYLKLTNFTKYTQEFMEE
                     NQENLKSRPNIIIDLRGNRGGDIQVMVNISGMFLPKKSVVATDHFRWYDHVYRSNKNQ
                     PLKYEKIIILQDENTASASENMIAALNDNLDNVELIGTKTFGKGIGQFTLPLRRGYAV
                     KATILKWLTPSGLNIQGNGIEPDIEYTGGDIIQYALSHIS"
     gene            complement(4169..>5713)
                     /locus_tag="PLG72_07795"
     CDS             complement(4169..>5713)
                     /locus_tag="PLG72_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ80737.1"
                     /translation="IIGENRYEWAVSYMAAVNGTGIVVPLDKDLPENEIENLLIRSKA
                     DAIIFSGHLKESISNIAKRIDFIKYYITMDEVSDGNGAESEKQESNHAERPIVFTELK
                     ELIEKGRKRVAEGCRDFIDAQIDPEAFSILLFTSATTDTSKAVMLCHRNIAENLMAMS
                     SMLNIVPDDIFLSVLPLHHTYENTCGFLCPFYRGAAVAYCEGLRHITKNLQESKATVM
                     LGVPLIYEAIYKRIWDQAAKHPGMLKKLKFGLKISGFMKKFGIDITRKLFAPIHNTFG
                     GNVRIFISGAAGIDPEVAEGFRNFGIHFVQGYGLTECSPIVALNRDVDFKDDAAGLPL
                     PGIEVRIDEAGSDGVGEIVVKGPNVMLGYYEDPENTAKAIRDGWFHTGDLGYIDSDGF
                     VHITGRKKNVIVTKNGKNIYPEEIETLLAKSPYIKECIVFGKDDPVYGDIVVSATIVP
                     DLDVIEAEHGGQRPGEEELHEIIRREIKEVNKSLVTYKYVKDFDLRFEEFAKTTTKKI
                     KRYVKN"
     assembly_gap    5714..5723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5724..5988)
                     /locus_tag="PLG72_07800"
     CDS             complement(<5724..5988)
                     /locus_tag="PLG72_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80738.1"
                     /translation="MKNIPLYEVRKIKSIKDMMETSVELYSDKTAFLYKPKGSDKYVP
                     VTFRQFKDDVDALGTALIDLGLKGGRIAIIGENPRGTGWNKYGL"
     gene            complement(6340..6543)
                     /locus_tag="PLG72_07805"
     CDS             complement(6340..6543)
                     /locus_tag="PLG72_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ80739.1"
                     /translation="MPDKTLICKDCHQEFVFTEGEQAFYKEKGFENEPQRCPECRRAR
                     KAQRRNSSFGSQPRNNYSTGSRW"
BASE COUNT         1663 a         1549 c         1621 g         1945 t
ORIGIN      
        1 cggcaggcat ccatactacc atgccgggct gtactatata caggaaccga gtgccatgct
       61 tccggggcag gtgctcggtg ctgagcccgg agacagggtg ttggacatgt gcgcggctcc
      121 aggcggaaaa accgttcaga tagctgcggc gatgaagggg agagggctgc tggtttcgaa
      181 tgacatcaat gcagagcggg taaaagctct tgtgaaaaat atcgaactat gcggcataag
      241 gaatgccatc gttttgaatg aatcgccgga gaacctggct cgcaatttta aaggatactt
      301 tgacaaaata ctgatcgatg ctccctgttc gggagaaggc atgttcagga aagacgagac
      361 agcggtcaga agctgggaaa gcttcaaatg cgaacgctgt tcgaaaatgc aggaagcgat
      421 cctccattat gcggatcttc tgctgaagcc tggagggacg cttgtgtact caacctgcac
      481 gttttctccg gaagagaatg agcagatgat tatcgatttt atactaaagc acaaaggcta
      541 tgaactcgtg gaaatcccga agctcgccgg gatcgaaggg ggacggcctg aatgggctgg
      601 aattgccgga aaacaggcgt gtaccccgga acatcaggtc ataggagaga atcctgaatg
      661 ggctcaggtc attggtgaag gtccatgtga tcgggacact gcggttggaa agcttgaaca
      721 gctcaccgat gatagttctt tccattgcgt tgactatgga gtcctggaat gtgccgccag
      781 gttatggccg cacaaagtca ggggagaggg tcactttgta gcgaagctgg tcaaaagggg
      841 aacttctcca gtacattttt ccggcagttt gtgtgttccc ggtgactcgc atatttccgg
      901 taacatgcat tatcccgaca gaaatgtgat taccggccat aattttacag gtgaaaccat
      961 ttccagtttc actgataaga ctgtctacaa ctttaaagga gaaaccgcct ccaatcttat
     1021 aggaggatct gtccccaacg accaggtaag gactgcttac ggctatgccg acaggttcag
     1081 ggagactgtg catgataccc ggaatgctat aaatgatatc cgtggtcaag gtggagaaac
     1141 ttcgaccaac tttatcgaca gcgtggagga aaccattcct tatgagaaac tgccggaggt
     1201 ctttcacaga ttcgtggacg agaacatgaa ctggttccct gacggcgtat ttttgctgaa
     1261 aggacgccat ctctatcacc tgcctgaacc accgccgttg ctgtcaggac taaaagttgt
     1321 gaaattcggt tggtttcttg gcactgttga agataacagg tttgaaccat cccattcatt
     1381 ggtcatggca ctggataaag gggcattcaa aaggacgctg gatcttggga cggagtcggc
     1441 agaggtcagg agctatctga aaggggaaac actgatattg aagggcgata aaggactgac
     1501 tgccgtttgt gttgacggaa agacgctggg atgggcaaag cagacgggag atatgctgaa
     1561 gaacatgtac cccaagggtt ggagaatgat gaagtaaaaa aggagtgaag tgataacttt
     1621 aatgagataa agattggtta tcgcggttaa gtgactagcc ccatgcagga tgaaggagtt
     1681 gagtaccggg atgaaaaaca ccgtacggct ggataagctg ctggctaact tcgggtttgg
     1741 cagcagaaat gatataaaaa agatggtaag gaatggcatg gtaacagtcg atggcgaagt
     1801 tgccagggat tcatccatgc atgtcgaccc ggaaaaaagc gtgatctcag ccaatggtat
     1861 gacgctgaat tacaggaagt ttatatatgt catgatgaac aagcctacag gtgttgtttc
     1921 ggccacatac gatgcgagac tgagaacggt gacagacctg ctgccggatg aactggcatg
     1981 tttcaacctt ttccctgcag gacggcttga tatcgatacg gaaggtttgg tcctcctgac
     2041 caatgacggc cagttggccc ataacctgct ttcacccaaa aagcatgtcg ccaaacgata
     2101 ctatgcgttg gtcgccggtc aggtcacaga gaatgatgtt gccgcctttt cagaaggagt
     2161 cgtactggac gacggatata aaacaatgcc tgccgaactg atcatcataa ggtcagggct
     2221 tcgttcggaa atagagttgg tcctgcatga gggcaagttc caccaggtca aaaggatgtt
     2281 cgaagcagtg gggaaaaagg tcctgtacct gaaaaggatc cagatcggcg gcctcaagct
     2341 tgatgaaacg cttgagcccg gcgaatgccg cgaactcacc cccgacgaac ttgaacaact
     2401 caaaagcggg gacacttctc acctttttga ctcctgaggt ggggacagtt ctcacctttt
     2461 tgtccttttc ggacaaggtg gttttctcag cagggacgct tctcaccttt ttatcttttt
     2521 cggtgaggcg tcagagggga cagttctcac ttttttgagt tcaggtttta cattcatgga
     2581 cggcagctga gattcataat aacagctatc ttctgttact gtcttacttc ttagtgggtt
     2641 tttagttatt tgcttagtat atatctaagt gaacacctta gcagatatca tggtaaatgt
     2701 ctaataaaac catctcctgc tgtcattagc atttcagagg gtaagagtta ttaaaaatga
     2761 cggttatttt ttatgaggaa atcaggcaga gatgcattat cactgcaaat ggattaggcc
     2821 atttcctttt gtcaaaaagg tgagaactgt ccccggcttt ttgatgaaaa ccggttcctc
     2881 attctgttga aaaggtgaga agtgtcccca aaaaactacc tgattaatca ggaaatatgg
     2941 ctgagggcat attggattat gtcgccgccg gtgtattcta tatcaggctc gatgccgttg
     3001 ccctggatgt tcaggcccga cggggtaagc catttcagaa tggttgcctt taccgcgtat
     3061 ccccgccgca gcggcagcgt gaactggccg atgcccttgc cgaatgtttt tgtaccgatc
     3121 agttccacat tatcgaggtt gtcattcagg gcggcgatca tgttctccga tgcgctggca
     3181 gtgttctcat cctggagtat tattattttc tcatatttca gtggctggtt cttgttcgag
     3241 cggtatacgt ggtcatacca cctgaaatgg tctgtagcca caactgattt tttaggcagg
     3301 aacatgcccg atatgttcac cattacctgg atgtcgccgc ccctgttgcc tctgagatct
     3361 attattatgt tcggcctgct tttgaggttt tcctggttct cttccatgaa ttcctgcgta
     3421 tacttggtga aattagtgag tttcagatag acggtcctgt catcgggaaa tgtgatttct
     3481 gactgcgccg cctcttcttt atccaactgt cgtgaccttt ccaggcttga cggggtatat
     3541 agatatgtat acctgtcgcc tctttcggcg cggatccttt cggttacagc tgcgatcaca
     3601 aagttgtcga aattgttgta ataccgtcct ttcgtatcca catccagata ctttttataa
     3661 aggtcgtcca gcgtttcagt gtagatatag ctgctggtga tgaaaatctt gaatgccaga
     3721 taatcataat tgaaaaatac gaacacgcct gttgccagga taaaaccgat aagaacgccg
     3781 agtgtcacgc gaaacctttt tctgtaccgt gaaaccagca gttcaggtgt catgattttc
     3841 ttactgcttt tttctgcttt tcccgtttta tttcccataa tactcactgt ttctccaatc
     3901 tgtcctgcag taactgattt taatcataaa tctgcttcgt atacctatcg atcattactg
     3961 tctgataaaa aggctcttat tggcgtaatt atccggtatg cagcggttat caataagtga
     4021 aaaccggctt tgctgttgca cgcacatatt ctgtgcccac atgtttccct gcggcctttt
     4081 cgacccggct tctctttagt gttatttgtc ccggttccat cgattactat gctttatcag
     4141 ttctgctgat gtaagttcct ctgtatcatc aattctttac ataacgcttg attttctttg
     4201 ttgtggtttt tgcaaattcc tcgaaacgca ggtcgaaatc cttgacgtac ttatatgtga
     4261 caaggctctt gttgacttct tttatttccc tgcgtatgat ttcatgcagt tcttcttcac
     4321 cgggcctctg gccgccgtgt tctgcctcga tgacatccaa atcgggaact atcgttgcgg
     4381 agacgactat atcgccgtat accggatcgt ccttgccgaa cacgatgcat tctttaatat
     4441 aggggctctt ggccagcagt gtctcgattt cctcaggata gatgttcttg ccgtttttcg
     4501 tgacgatgac gttctttttc ctgccggtga tatgaacgaa tccgtcgctg tcgatatagc
     4561 caaggtcgcc cgtatggaac cagccgtccc tgatggcttt tgcggtattc tcgggatctt
     4621 cgtaatatcc gagcattacg ttgggccctt taacaacgat ttctccgaca ccgtcgcttc
     4681 cagcctcatc gattcttacc tcaatgccgg gcaaaggcag tcctgctgca tcgtctttga
     4741 aatcaacatc cctgttcagc gccacgatgg gactgcattc agtcagcccg taaccctgca
     4801 cgaaatggat gccgaaattc ctgaaacctt cagccacttc gggatcgata cctgcagcgc
     4861 cgctgatgaa tatccttaca ttgcctccaa atgtattgtg tatcggagca aacagtttcc
     4921 ttgttatatc tataccgaat ttcttcataa agccgctgat cttcaggccg aacttcagtt
     4981 tcttcagcat tcccggatgc ttcgcagcct gatcccagat ccttttgtaa atcgcttcat
     5041 atatcagcgg aaccccgagc ataacagtcg ctttcgattc ctgcaggttt ttggtgatat
     5101 gcctcagacc ttcgcagtat gcgactgctg ctccgcggta gaaggggcag aggaaaccgc
     5161 atgtgttttc gtatgtatga tgaagcggaa ggacagaaag gaaaatgtca tcaggaacga
     5221 tattgagcat cgaactcata gccatgagat tctccgctat gttcctgtga cagagcataa
     5281 ccgcttttga cgtatctgtg gttgccgaag tgaagagaag gatactgaat gcttcaggat
     5341 ctatttgggc atctataaag tccctgcagc cttcagccac tctttttctg cctttttcaa
     5401 taagttcttt caattctgta aatacgatgg ggcgttcagc atgattactt tcctgttttt
     5461 ccgattcagc tccatttcca tccgacactt cgtccatcgt tatgtagtat tttatgaaat
     5521 cgatgcgctt tgctatgttc gagatacttt ccttaagatg gcctgaaaat attatcgcgt
     5581 cagcttttga cctgatgagc aggttctcga tctcattctc ggggagatcc ttgtccagcg
     5641 gcaccacaat cccggtgccg tttactgccg ccatgtatga aaccgcccat tcgtagcggt
     5701 tctcgccaat gatnnnnnnn nnnacagtcc gtacttgttc cagcctgtac cacgagggtt
     5761 ctcgccaatg atggctattc ttccgccttt aaggccgaga tcgatcaatg cagtgccaag
     5821 tgcgtcgaca tcgtctttga attgtctgaa cgtaacaggc acgtatttat cgcttccttt
     5881 cggcttatac aggaatgcgg tcttgtcgga gtacagttcg acactggttt ccatcatatc
     5941 ctttatcgat ttgatctttc tgacttcata caacggaatg tttttcataa aaccacctca
     6001 ggtatatttg caagcattat ctgcattata gcttacaggg catgtaaata ttatatatca
     6061 tttgttacaa gtgtaaaaga aaagaaggca attggtgtgt aaagatgttt tttgccggaa
     6121 attatttctc atcgatccct ggcatggagg gcacagaaac gggagctttc tgcatcggta
     6181 tcctcttcat tggaatttat gcataagaaa ggagagtctt ttgactctcc ttaaagcatg
     6241 cgcttattgt catctgaata agtctgcaac ctgcagttta ccgttttcaa taagtctgcg
     6301 tctgcaatct gccgcctgga acaggcctgc acctgcagat taccacctgc tgccggtgga
     6361 gtaattgttt cttggctgac ttccgaagct gctgttcctt ctctgagctt tcctggccct
     6421 cctgcattcc gggcaccttt gaggctcgtt ttcgaatccc ttttccttat agaaggcctg
     6481 ttcaccttcc gtaaagacga attcctgatg gcaatccttg caaatcaatg ttttgtcggg
     6541 catattttta cctcctggtc gatctggatt taactggttt ggacttaatt cctctgttat
     6601 ccaaaaagac taggaggttt cggtcggcaa actaattaaa tctcttgata ttctgtatct
     6661 gacgatacgt acatattata ccactataat ctgaaagtta caagcaaaat ttggaagact
     6721 agtaaattat cctcctgacc acgatgtcgg accggatgcg ctgccacacc gcgggatcct
     6781 ccatgcca
//
LOCUS       DAOSCC010000134         6769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7236_length_6769_cov_4.065833, whole genome shotgun sequence.
ACCESSION   DAOSCC010000134 DAOSCC010000000
VERSION     DAOSCC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7236_length_6769_cov_4.065833"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLG72_07810"
     CDS             complement(<1..373)
                     /locus_tag="PLG72_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ80740.1"
                     /translation="MLFDTHAHLYDTQFAGDLDEVVSRAKENGVGYILTASENVASSV
                     ENITLTQKYDIFYAAVGIHPHNAVNQNNSIISVLKEFASYPKVVAIGEIGLDYYYDNS
                     PRDVQKTWFAKQISLAGELKLP"
     gene            complement(610..2667)
                     /gene="metG"
                     /locus_tag="PLG72_07815"
     CDS             complement(610..2667)
                     /gene="metG"
                     /locus_tag="PLG72_07815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ80741.1"
                     /translation="MERKTFYITTPIYYPSDKLHIGHSYTTVAADAIARYKRLRGYDV
                     MFLTGTDEHGQKIQRKAEEKGVTPKQYVDEIVAGIKELWKLMKITNDKFIRTTDKEHE
                     VTVQKIFKKLYDQGDIYKSEYEGWYCTPCESFWTETQLKDGKCPDCGREVELTREESY
                     FFRLSKYQDRLIKYIEENPDFIQPVSRRNEMLNNFLLPGLEDLCVSRTTFNWGIPVTF
                     DEKHVVYVWIDALSNYITALGYLSDDDSAYRKYWPADVHLVGKEIVRFHTIIWPAMLM
                     ALGEPLPKQVFGHGWLLLEGGKMSKSKGNVVDPVILINKYGLDPIRYFLLREVPFGSD
                     GVFSNESLIQRINSDLANDLGNLLNRTVAMIEKYFGGTIPGQREEGEYDSDLRDTFAK
                     LPYTVEELMDKFQFSTALAEIWKAISRANKYIDETMPWVLAKSEESKPRLAAVMYNLA
                     ESLRIVSILIQPFMPETPAKMWEQLGISDSAAIAWDSAKQWGGYPAGAAVQKGEALFP
                     RIDMKKELEELETMSAKGKESAASGGHAQGGQDTSGDSGKASGSGQAEGCKAAGVRNA
                     AGAGKADDKNTDGTSGFITIEDFAKLDLRAAKVLEAEKIEGSDKLLKLKLELGSETRQ
                     VVSGIAKHYTPESLIGKSVILVANLRPARLRGVESQGMILAASNDSGLVVATLDGEIS
                     SGS"
     gene            2859..3968
                     /locus_tag="PLG72_07820"
     CDS             2859..3968
                     /locus_tag="PLG72_07820"
                     /inference="COORDINATES: protein motif:HMM:NF022233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2600 family protein"
                     /protein_id="HOJ80742.1"
                     /translation="MGKLTETAAFYAKFAGRVLPEACRQRSRVGEELGHCLPVPLPST
                     DETEEDPLSGMFAVRQACCFLFSICPGADYRDLVPFISSLHLIAVTLEKYRKKKNITD
                     EKEIRDLLGCLSAAADPSRNLPQSGIPGILACNMQDADTGRRDTHAKNRPPLMCHAEQ
                     CRLKLAVLPSYTQVSAKIKKYMQMYIDVQSYRHYPPIISMDLLKTWSSNYLKRYRDIS
                     CWELCAAADSFLGIAAMFAAASVPDITEEEVLLLDEFCFPWLCGFCSMLGSVVSLRMW
                     PDSDGLNFSSFYKSLKECEERLIFFAENTGAACLKLRNSSLYLLIMKAAAGLYLSDPE
                     AGFGMLRLTVSNIVRKTSLRPYRYAGSLLRALRLL"
     gene            complement(4129..4668)
                     /locus_tag="PLG72_07825"
     CDS             complement(4129..4668)
                     /locus_tag="PLG72_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ80743.1"
                     /translation="MKSSIFAAISMYILPVVILVILGAGLIRKVKIFEAFTEGAAEGI
                     TTMARIMPSLVGILTAIGVFRASGALDVVVGLLAPAASLLGIPSEALPLVLMRPVSGS
                     ASLAIVSDIISKYGPDSYVGRVACTMLGSTETIFYTLAVYYGSVGVRNIRYTLAAALI
                     ADGVSAVASAFICALVFGR"
     gene            complement(5072..5629)
                     /locus_tag="PLG72_07830"
                     /pseudo
     CDS             complement(5072..5629)
                     /locus_tag="PLG72_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
     gene            6002..6244
                     /locus_tag="PLG72_07835"
     CDS             6002..6244
                     /locus_tag="PLG72_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ80744.1"
                     /translation="MKSTGIVRKVDELGRVVLPIELRRTLDIAEKDALEIYVDGSTII
                     LKKYEPACIFCGDAKDVTTYKGKNICPNCMKELRKQ"
     gene            complement(6365..>6769)
                     /locus_tag="PLG72_07840"
     CDS             complement(6365..>6769)
                     /locus_tag="PLG72_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ80745.1"
                     /translation="RETRTMVFYEAPHKLIHTLKDLRDLLGNRRIAIARELTKKFEEV
                     WRTTLDEAIARYGNEPPKGEFVIILEGADERGLHEEEVKSWEEVSIEEHVRMYMDSGL
                     DRKEAMKKVSEDRGISKRDVYNSLLGKDRGRQ"
BASE COUNT         1518 a         1886 c         1582 g         1783 t
ORIGIN      
        1 caggcagctt cagttcccct gcaagcgata tctgttttgc aaaccaggtc ttttgcacat
       61 cacgtggtga attgtcataa tagtaatcaa gacctatctc gccgatagca actactttcg
      121 gatatgatgc gaattccttc agtaccgata tgatgctgtt gttctggttg accgcgttgt
      181 gcggatgtat cccgacggcg gcataaaaaa tgtcgtattt ctgggtcagc gtaatatttt
      241 caactgacga ggctacattt tcagacgctg tcagtatata tccgaccccg ttttcctttg
      301 cccttgatac cacttcgtcg agatcgccgg caaactgtgt gtcatacaga tgtgcatgcg
      361 tgtcaaacaa catatgaaac ctcgcgtaag ctcactggta cttgccttaa ttatatctta
      421 ttatcccgga cactgcaaca aagccgcctg taactgtatt caggcggctt tcgatccgtt
      481 gttccctgca gtgttcagtc gatcacggag ccattgtccc tgatgtctgc caggcaaccc
      541 tgggaccgct ctgtccgcag cggtgcccgg acggccccgg agccccgttc caggcaatac
      601 tcaggaaact tatgaaccac tgcttatttc accgtcaagc gtagcgacga ccagaccact
      661 atcattcgat gcggcgagga tcattccctg cgactccacg cctctcagcc tggcaggcct
      721 gaggtttgcc acaagtatga ccgatttgcc gatgaggctt tcaggggtgt aatgctttgc
      781 tattccggac acgacctgcc tggtctcgct gccaagctcc agtttcagtt tcaggagttt
      841 gtccgacccc tctatttttt cggcctcgag tacttttgct gccctgagat ccagtttcgc
      901 aaaatcttct atcgttatga agccgctcgt tccgtctgta tttttgtcat cagctttgcc
      961 ggctccggcg gcattacgga cacctgcagc cttgcagcct tcggcctgac cggaacctga
     1021 agctttgccg ctgtctccgg aagtatcctg tccgccttgt gcatgcccgc cgcttgctgc
     1081 gctttccttt ccttttgccg acattgtctc aagttcctcg agttcctttt tcatgtctat
     1141 cctcgggaaa agtgcctcgc ctttttggac cgcagcgccg gcaggatagc cgccccactg
     1201 cttagcgctg tcccaggcga tagcagcgct gtccgaaata ccaagctgtt cccacatctt
     1261 agccggagtc tcgggcatga acggctgtat gaggatagac acgatcctca ggctctctgc
     1321 aaggttgtac atgacggcgg cgaggcgtgg tttgctttct tcggatttcg ccagcaccca
     1381 tggcatcgtt tcatcgatgt atttattcgc cctcgatatg gccttccaga tttctgccag
     1441 cgcggtgctg aactggaact tgtccataag ctcctccacc gtatacggca gttttgcaaa
     1501 agtgtccctc aggtcgctgt cgtattcccc ttcttccctc tgtccaggta tggtgcctcc
     1561 gaagtatttt tctatcatgg cgacagtcct gttaaggagg ttgccgaggt cattggcaag
     1621 atccgaattg atcctctgga tcagcgattc gttggagaat acgccgtcgg atccgaaggg
     1681 gacttccctg aggaggaaat accttatcgg atccaggccg tacttgttga tcagtatgac
     1741 cggatcgacc acattcccct tggatttgga catcttgccg ccttccagca gaagccagcc
     1801 atggccgaac acctgcttcg gcagcggctc gccgagagcc atcagcatcg ccggccatat
     1861 gatcgtatgg aacctgacta tctccttgcc caccaggtgg acatccgcag gccagtattt
     1921 gcggtaggcg gaatcgtcat ccgaaaggta gcccagcgcc gttatgtagt ttgaaagcgc
     1981 gtctatccac acatagacca catgcttttc gtcaaatgtc accgggatgc cccagttgaa
     2041 cgtcgtcctc gatacgcaga gatcttccag gcccggcagc aggaagttgt taagcatctc
     2101 gttgcgcctc gacacgggct ggatgaaatc ggggttctct tctatatact ttatcagcct
     2161 gtcctggtat tttgacagcc tgaagaaata gctctcttcc ctggtaagct cgacttctct
     2221 gccgcagtcg ggacatttgc cgtccttcag ctgcgtttct gtccagaatg attcgcacgg
     2281 cgtgcagtac cagccttcat attcgctttt ataaatatcg ccctggtcgt aaagtttttt
     2341 gaatatcttc tgcacggtga cctcatgctc tttgtcggtc gtccttataa acttgtcgtt
     2401 ggtgatcttc atcagtttcc agagctcttt tatgccggca actatttcat ccacatactg
     2461 cttcggagtc acgcctttct cctcggcttt gcgctgtatt ttctggccat gttcgtctgt
     2521 tccggtgagg aacatcacgt catagccacg gagcctcttg tacctcgcga tcgcatctgc
     2581 agccacggtt gtatatgaat gccctatgtg cagcttgtca ctcggataat atattggtgt
     2641 ggtgatataa aaagttttcc tttccatctc attgcctcca tgtccctttt agcttacggt
     2701 ttgccgcaat atttaatata accggcctct aaccatttca gactttattt taatatacat
     2761 tttggacata tatttttcaa gtgatatttg gtaccggcgg aatatatcac cgggacggaa
     2821 aataaaatct aaacagatcg gtgaagggag ccgggcccat gggaaagctg accgaaactg
     2881 ccgcatttta tgcaaaattc gcaggcaggg tgctgcccga agcgtgcagg cagcgcagcc
     2941 gcgtcgggga agaactgggg cactgcctgc ctgttcccct ccccagcacg gatgaaacgg
     3001 aagaagaccc gctgtcaggg atgtttgccg tcaggcaggc atgctgcttc ttattctcga
     3061 tatgcccggg cgccgattac agggatcttg ttccattcat atcatcgctc catctcattg
     3121 ccgtaaccct ggaaaaatac aggaaaaaaa agaacataac agacgaaaag gaaatccgcg
     3181 accttttagg gtgcctttcg gcagcggcag atccttcgag gaacctcccg cagtcgggta
     3241 tccccgggat ccttgcatgc aatatgcagg atgcggatac aggacgccgg gatacacatg
     3301 ctaaaaaccg accgccactg atgtgccacg cggaacagtg caggctcaag ctggctgtcc
     3361 ttccctcata cacacaggtt tcggccaaaa taaaaaagta catgcagatg tacatagacg
     3421 tacagtcata caggcattat cctccaatca tcagcatgga cctgcttaaa acatggagca
     3481 gcaactatct caaacgctac cgcgacattt cctgctggga actgtgcgct gcagccgatt
     3541 ctttcctggg gatcgcggca atgtttgcgg ccgcatccgt tccggacatc acggaagaag
     3601 aggtgctctt gctggatgag ttctgtttcc cgtggctctg cggtttttgc tcgatgctcg
     3661 gttcggtggt cagtttaagg atgtggcctg attcggacgg actgaacttc tcttcattct
     3721 acaaaagcct taaggaatgt gaagaaaggc tcatcttttt cgcagaaaac accggggctg
     3781 cctgcctgaa actcaggaac agcagccttt acctgctcat aatgaaagcc gccgcagggc
     3841 tttacctgtc tgatcccgaa gcaggtttcg gaatgctgcg tcttaccgtg tcgaacatag
     3901 tccggaaaac atcgctgcgt ccataccgtt atgccggttc cctgctgcgc gccctccgcc
     3961 tgctttaaac ggggacgttc cttcaaatta cccgtccgcc gaaagccgga cggctcctgc
     4021 attccggctt tattagccga ataaaagata aacagcgaat ggcaggggac agttcttcac
     4081 ttaacaagga cgttaaccaa acccgtacct ggaacgctgc ccggatccct acctgccgaa
     4141 aaccagcgcg catatgaacg ccgacgcgac tgcgctgacg ccgtccgcga tcagtgccgc
     4201 cgccagtgta tatcttatat tcctgacgcc gacagagccg tagtagactg caagagtata
     4261 gaagatggtc tcggtagatc cgagcatcgt gcatgccact cttcccacgt atgaatccgg
     4321 gccgtatttg ctgattatat ctgagacgat cgccaatgac gcactgcctg aaaccggcct
     4381 catcaagacg agtgggagag cttccgacgg tataccaagc agcgaagccg ccggagccag
     4441 caatccgaca acgacgtcca gcgctcctga cgccctgaag acgcctatgg ccgtaaggat
     4501 cccaaccagc gacggcatta tcctggccat ggtcgtgatc ccctcggcgg cgccttctgt
     4561 gaaagcctcg aatattttga ctttccggat aaggcccgcg ccaagtataa ccaggataac
     4621 cacaggaagt atgtacatgg atatggcagc aaatatcgag gatttcatac cgtcgtcaac
     4681 ctgtccccgt ttttcctcct gttcctgcgc tcgttcctgt ccgcagccgc gtttccgttc
     4741 ttatccctgt gcccatttcc gcttaaaaac ctgaactgac cctgcctcca ttaccgcagc
     4801 tgtctctctg gcgatcataa actttcctgt tacccgaacc gggtccgatc ctgataccgg
     4861 gaccggatcc tttcccgctt cctgggccgc gcccgatacc agctccattt attccggcat
     4921 ggtacagccc gtttcgcgtt gccgaaccgg atcctgcatt ccgtggcctg ccgtgcctgg
     4981 catatgcatt cggcagggac atccccgtat ttcgggccga tgccgcgccg gcattcgata
     5041 aagcaggcat tccggccttc cacgccagca tcagcacttt agctgccagt atcccggcaa
     5101 acgcagcgca cagtgaagcc gcccatatgc aaggtatgat ctcaaccggg gctttagaac
     5161 cggcatcgct cctcaatgct atcacagttg tcggtataag ctgcagcgct gatgtgttca
     5221 gcacaaggaa catgcacatg gcgtcggtgg cagtatcctt tttcctgttc accttttgca
     5281 attcttccat agcctttatc ccaaatggcg tggcagcatt gcccaacccc aggaaatttg
     5341 ccgacaggtt catgacgatg gcgcccatgg ctttatcgtt gccgtcaaga tccggaaaaa
     5401 ccggcctgag aatgaatttt gcagctttcg ctataaaatt tatcaatccg cttttttcca
     5461 ttatcttcat caggccggac cagagacaca tgatcccgag cagcccaagg cccagttcca
     5521 cggccttgcc ggccgaatcg aaggctgctt tggtcacttc gccgaccctg ccgtttatga
     5581 tgcctgccag gagaccgacg gccagcagcc ccgcccagat ataattcata tactgcctcc
     5641 tcacaatttc caaccggtca attatttata ttgagaatgc tgttccaaaa ttacaggaaa
     5701 ttgtacccga aactgccaaa cacgatgtcg aattttgcat aatattttct ttcggttttt
     5761 ccagcagatt ttacggtttt gcaaaccgcg aaaaaacgcg attcttatgt aatgaaaata
     5821 ttcccatttc tcaaacttgc tttggttccg ggctttgacc gatcacagcg tattgttttc
     5881 acttaatttt ccataacaat tttttgaata tatataaaat aattgttgac tcaaactgga
     5941 atttatgcta gtatattttt ataaaagaat gtcgaaatat gttagtaaag gaggaaagag
     6001 tatgaaatcc accggaatag taagaaaagt tgacgagctt ggtagagtag tcctcccgat
     6061 cgagttgagg agaacacttg atatagccga gaaggacgca cttgaaatat atgtcgatgg
     6121 atctacgatc atattgaaga aatatgaacc ggcatgtatc ttctgcggcg acgccaaaga
     6181 cgtcactact tataagggta agaatatctg cccgaactgc atgaaagaac tcaggaaaca
     6241 ataatcggtt cggtaaagat gcacatattc acagagaaat acgtttcaca gacagtgcgc
     6301 aaaatgtaca ggcactgagc atccaaaaac agtgaagccc ggctttgcag tcgggttttt
     6361 tgttttactg cctcccccgg tctttcccca gaaggctgtt gtacacatct cttttgctga
     6421 ttccccggtc ttccgacact tttttcattg cttctttcct gtcgagcccg ctgtccatgt
     6481 acatcctgac gtgttcctct atactgacct cttcccagct ttttacttct tcttcatgca
     6541 gtccgcgttc gtccgcgccc tccaggatga tgacgaattc gcctttcggg ggttcgttcc
     6601 cgtacctggc aatagcctcg tcaagcgtgg tccgccagac ctcctcgaac ttcttggtca
     6661 gttcccttgc aatcgcaatg cgcctgttgc caagtaaatc ccttagatcc ttcagcgtat
     6721 gtatgagctt gtgaggcgct tcatagaaca ccatggtcct ggtttccct
//
LOCUS       DAOSCC010000135         6759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7247_length_6759_cov_4.187947, whole genome shotgun sequence.
ACCESSION   DAOSCC010000135 DAOSCC010000000
VERSION     DAOSCC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7247_length_6759_cov_4.187947"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1382)
                     /locus_tag="PLG72_07845"
     CDS             complement(117..1382)
                     /locus_tag="PLG72_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ80746.1"
                     /translation="MYSRRRYGSASILITSVLTSMAVSLLVLFAVPRLITVADSDERG
                     NKAPGARLQYSGSGAGNLNGNAGQQDPNGTGWSEQGGNTAENGYMTGSTADYAASIPV
                     TQRVAKKATAGVVGITVERAVKENLFSRNRETEVGFGSGVIVSSDGYILTNYHVAGGK
                     SNRIVVSLADGRYVDGEVVWSDPVLDLAAVKVNLTGLTPLALGDATKLEVGEHAIAIG
                     NPLGLDFQRSVTSGIISALNRTIRIETEDGVNYMEDLIQTDASINPGNSGGPLLNSRG
                     EVVGINTVKVTSAEGMGFAIPINIVKPVVARLMETGTFEEPYMGIFAYDGQTIPYIDG
                     QVQIQNGIYVAHVDRNGPAYKAGIREGCIIARVDDNEINTMMQLRTYIYTKLPGDTVR
                     IYHKKRGSDEWITSELVLARKERDGLVTR"
     gene            complement(1496..1726)
                     /locus_tag="PLG72_07850"
     CDS             complement(1496..1726)
                     /locus_tag="PLG72_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOJ80747.1"
                     /translation="MADRYRLGDIVEFRKQHPCGSKQWEVMRTGADFRVKCLGCGHQV
                     MMPRPKFEKSVKKIIRPFLSADTSIDTTDENK"
     gene            complement(1791..3125)
                     /gene="glnA"
                     /locus_tag="PLG72_07855"
     CDS             complement(1791..3125)
                     /gene="glnA"
                     /locus_tag="PLG72_07855"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOJ80748.1"
                     /translation="MSRYTKEDIIKIVRDEKVEFIRLQFTDIFGMLKNVAITAKQLEK
                     ALDNKCMFDGSSIEGFVRIEESDMYLSPDTDSFVILPWNSEIGKTARLICDVYTADGK
                     PFEGDPRYVLKRALNKAARMGYDTFNVGPECEFFLFLVDDEGNPTTITHDKAGYFDLG
                     PVDLGEKARRDMCLTLEEMGFEVEQSHHEEAPGQHEIDFKYTDALNAADAIQTFKLVV
                     KTIAQKHGLHATFMPKPVYGIAGSGMHVNTSLFNKGKNVFYDENGPLELSQEAYWFIG
                     GLMKNIRSITALTNPLVNSYKRLIPGYEAPVYIAWSARNRSPLIRVPSARGTATRLEL
                     RCPDPSCNPYLALAAILSAGLDGIENRIQPPEPVNRNLYDMSYEDRCAGQIGALPASL
                     EEAICEMRQSEFARELLGDHIFNKYIEAKLKECEGYKTQISKWEIDAYLKNY"
     gene            3489..5468
                     /locus_tag="PLG72_07860"
     CDS             3489..5468
                     /locus_tag="PLG72_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ80749.1"
                     /translation="MQKSKYLTALLCAAAVALTSTAALNAVPFSFAGTEEADGTLRTK
                     GSKIVDPTGREVRLYGLNCPSLEWIHTGEHMPEAVITAFDQWEANLVRLPMNQDFWFG
                     YDQSQTDGGKAYRRLISEIVDIAAERKKYIWLDLHWSNGGTEWGMNTGQHKMPDKNSL
                     VFWESVAKEFRNHPYVLFGIYNEPHDISWDVWKNGGLVFESVEVTENGKKTSKSIKYE
                     AVGMQALIDAIRATGANNIVIVGGIDWGFDLSKAADEKYRLVDTPEGNGIVLDTHPYP
                     WKNKNWSLLIDKAGKHYPIIVGEFGTGPDKNDPDKPDETYFKNYHETLFNWIEEHNYS
                     FAAWSFHPGAGPCIIRDWSYEPTPFHGVYVRDFLIKAASRGVSLFDGNNYTGKSIDIK
                     PGKYTSADLKAAGMDPASIKSVKLNNDHSLAYKLTLFSNDDFRGSSQTCIYNIPDLQA
                     LLADKTVKSLKLETVKLENINKGSSVECSSGNDQSGAVTDGDETTRWIVDEEGPKWVI
                     TDLGTVCTIHRIILKHASGKNLNKHNNNQDFKVSISADGTSWTEVYAATGNKDYESVI
                     DFEPSAARYVKVDIMKGSQIPPHTRATISEIEVYGLKGSETAGERSAETQGGDGNGSF
                     AGNPWIWLAFSGLASILVIALTVLSRRKERIRGTA"
     gene            complement(5673..5834)
                     /locus_tag="PLG72_07865"
     CDS             complement(5673..5834)
                     /locus_tag="PLG72_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOJ80750.1"
                     /translation="MDRYVCTACGYVYDPAVGDPDGGIAPGTPFDDIPDDWVCPLCGV
                     GKDMFEKEQ"
     gene            complement(5936..6460)
                     /locus_tag="PLG72_07870"
     CDS             complement(5936..6460)
                     /locus_tag="PLG72_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80751.1"
                     /translation="MKRSGRVLGGILVALGILFLIRNVDIFEPVWRIINPGALLAKFW
                     PSLFLLLPGALFHYGYFSGSRRNPGLLVPGGILLVLGLVFQFNMLFGGWDITWPLIIF
                     SIAFGLFELYIFGNREKGLLIPIGILSGLSFLFFFSFSLSRLLSFNTKPYAMPVLLIG
                     VGIILVSGGRKKHV"
     gene            complement(6578..>6759)
                     /locus_tag="PLG72_07875"
     CDS             complement(6578..>6759)
                     /locus_tag="PLG72_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1256 domain-containing protein"
                     /protein_id="HOJ80752.1"
                     /translation="TGIVNFGGFMDFLILQNTRLHVVMRMADLIASGIRFVIWKLEKS
                     GLLLTDRSEAGTAEK"
BASE COUNT         1724 a         1812 c         1582 g         1641 t
ORIGIN      
        1 tcattggaac aaatgagccg gccagcagct gtccgcgaaa catacccaat gctggatcgg
       61 ccgccccttc gctaaagtcc agcaacaaaa cttaataact tgtcgggtca caatcatcac
      121 cttgtgacga ggccgtctct ttccttgcgt gcgagcacaa gctcagaagt gatccattcg
      181 tcgctgccgc gctttttatg gtaaatacgc acggtatcgc cagggagttt cgtatagatg
      241 taagtcctca actgcatcat cgtatttatc tcattgtcat ccacccgcgc gatgatgcat
      301 ccttcccgta tccccgcctt atatgcaggc ccgttcctgt caacatgggc gacatagata
      361 ccgttttgga tctgcacctg gccgtcgata tacggtatgg tctgcccatc gtacgcgaat
      421 atccccatgt aaggctcttc aaatgtgcct gtctccataa gccttgcaac gacaggcttc
      481 acaatgttga tcggtatggc gaagcccatc ccctcggcgc ttgtcacctt tacagtattg
      541 attccgacga cctctcccct tgagttgagc aggggcccgc cgctgttgcc aggatttatg
      601 ctggcatccg tctgtatcag gtcttccatg taatttacgc cgtcttccgt ttctatcctg
      661 atggtcctgt tgagagcgct gatgatgcct gatgtgactg atctctggaa atcaaggccc
      721 aaaggattgc cgatggcaat ggcatgctcg cccacttcaa gcttagtcgc atcgccaagt
      781 gccaatggcg tcaggcctgt aaggtttact ttcacggccg ccagatccag tacgggatcc
      841 gaccatacga cttctccgtc gacgtacctg ccgtcagcca gcgacactac tatcctgttg
      901 ctctttcccc cggctacatg ataattggtc aggatatatc catccgaact gacaattact
      961 cctgaaccga atccgacttc cgtctccctg ttcctgctga aaaggttttc ctttacggcc
     1021 ctttccactg ttatgccgac aacacccgca gtagcctttt tggctactct ctgggtcaca
     1081 gggatactgg ccgcatagtc tgccgtactg ccggtcatat aaccattttc cgccgtattc
     1141 ccgccctgtt cgctccagcc ggtaccattc ggatcctgct gccctgcgtt cccgttgaga
     1201 ttcccggccc cgcttccgga atactgcagc cttgctccag gagccttatt gcccctttcg
     1261 tcgctatcag cgacagtaat aagcctggga acggcaaaaa gcaccaggag gcttacggcc
     1321 attgaagtaa gtacggatgt gatcaggatg gacgccgacc cgtatcgtct tctgctgtac
     1381 atatatcatc aaccccgttt tcgctttatg acagtcatgc ttattatttt ccgcggtgtc
     1441 ataaagaata cggaaagcgg gcagaaacat atacagtagc aatatttcca tcctgctatt
     1501 tgttttcgtc tgtcgtatcg atcgatgtgt cggccgaaag gaaaggcctg atgatctttt
     1561 ttacgctttt ctcaaatttt ggtctgggca tcatgacctg gtgtccgcat ccaaggcatt
     1621 tgaccctgaa atcggcgccg gttctcatga cttcccactg tttgctcccg catgggtgct
     1681 gttttcggaa ttcaactata tctcccagac ggtacctgtc cgccatttac atacaccacc
     1741 gttccatctg ctccttcagc ctgtacgaat ggctgccgga acgatacata tcagtagttt
     1801 ttgagataag cgtcgatttc ccacttgctt atctgcgtct tgtatccttc acattccttc
     1861 agctttgctt cgatatactt attgaatata tgatctccaa gcagttccct tgcaaattca
     1921 ctctgcctca tctcacagat ggcttcttcc aggcttgccg gcagggctcc gatctgccct
     1981 gcgcatctgt cctcgtagct catgtcgtac aggttcctgt ttaccggctc gggaggctgg
     2041 atcctgttct ctatgccgtc aaggcctgcg gacagtatcg cagccagtgc cagatacggg
     2101 ttgcagcttg gatcgggaca tctgagttcg agcctcgtcg cggtcccgcg ggctgaaggc
     2161 accctgatca gcggactcct gttcctcgct gaccatgcta tgtaaaccgg ggcttcatat
     2221 cctggtataa gccttttgta tgaattgacc agggggtttg tcagcgccgt tatggaacgg
     2281 atattcttca tcagtccgcc aatgaaccag taagcctcct ggctgagctc cagcgggccg
     2341 ttctcatcgt agaagacatt ctttccttta ttgaacagcg aagtgttgac atgcattccg
     2401 gaacccgcta tgccgtatac aggctttggc atgaacgtag cgtgaaggcc gtgtttctgt
     2461 gcaatcgtct tgaccaccag cttgaatgtc tggatcgcat cagcagcatt cagtgcgtcg
     2521 gtatatttga agtcgatctc atgctggccg ggcgcttcct catggtggga ctgctccacc
     2581 tcaaacccca tttcctccag ggtgaggcac atgtccctcc tggccttctc gcccaggtcc
     2641 acgggaccaa ggtcgaaata ccccgctttg tcgtgggtga tcgtagtggg gttcccttca
     2701 tcgtcgacaa ggaaaaggaa aaactcgcat tcaggaccga cattgaacgt atcgtatccc
     2761 attcttgctg ctttattcaa agccctctta agtacatacc tggggtcccc ttcgaagggc
     2821 tttccgtccg cggtgtacac atcacagatg agccttgcgg tcttcccgat ttcagaattc
     2881 catggcagta tgacgaacga atccgtatca ggcgacaggt acatgtcaga ctcttcgatc
     2941 cttacaaatc cttctatgga tgagccgtcg aacatacact tgttgtcaag agccttttcc
     3001 agttgctttg ccgtgatcgc cacattcttc agcatgccga agatgtcggt gaactggagc
     3061 cttatgaact cgaccttctc atccctgact atttttatga tatcctcttt cgtgtatctg
     3121 gacatctggt acacctccgc gatataaaaa aacacactca ggcaaacctg gatatcgttc
     3181 aggaactctg ctgctaaaac cagactggat ataaactgcc gcagttgcgc cgaaccggat
     3241 gccgccggta tccggcaaaa atctatctct attttaccat gaaaaaggaa cagggcaata
     3301 ttaatttagg ttttaagcac cacccagggc agtttgactt aaaaaccgat ttcctgttcc
     3361 atgacccgaa agctgcatat tcagcgtaaa gaaaactgtt tttaaagcac ttgtccgtcg
     3421 cgatggtata ataatattgc aggcaggatc ggcacccgcc gcttattttc gttcaggggg
     3481 caataaaaat gcagaagtca aaatatctaa cggctttatt gtgcgcggca gcagtagctc
     3541 ttacatcaac ggcagctctg aatgcagttc ctttcagctt cgccggtacg gaggaagctg
     3601 acggcacgct ccgtacaaag gggagcaaaa tcgtggatcc caccgggcga gaggtacgcc
     3661 tttacgggtt gaactgcccg agccttgaat ggatccatac aggcgaacat atgcccgaag
     3721 ccgtcattac agcctttgac cagtgggaag ccaacctggt aaggctgccg atgaaccagg
     3781 acttctggtt cggatacgat cagtcccaga cagacggcgg gaaagcatac cgcaggctca
     3841 tatcagagat cgttgacatc gcggcggagc gcaaaaaata catctggctg gatctgcact
     3901 ggtccaacgg cggcaccgaa tggggcatga ataccggcca gcacaagatg ccggataaaa
     3961 acagccttgt attctgggaa tcagtcgcaa aagaattcag gaaccatcct tatgtactat
     4021 ttgggatcta taacgagcct catgacatat catgggatgt ctggaaaaac ggaggcctgg
     4081 tttttgaatc ggtggaagtg actgaaaacg gcaaaaagac atcgaaaagc atcaagtatg
     4141 aagctgtcgg catgcaggcc ctcatcgacg ccatcagggc gacaggcgca aacaacatcg
     4201 taatagtcgg gggcatcgac tggggcttcg atctgtccaa agccgcagat gagaagtaca
     4261 ggctcgtgga tacgcctgaa ggcaacggca tagtcctcga cacccatcca tacccgtgga
     4321 aaaacaaaaa ttggtcgctg cttatagata aagccggaaa acattatccg atcatcgtag
     4381 gcgaattcgg caccggaccc gataaaaacg atccggacaa acccgatgaa acctacttca
     4441 aaaactacca tgaaacgctt ttcaactgga tcgaggaaca caactacagc tttgccgcct
     4501 ggtcattcca tccgggcgca ggtccatgca ttataagaga ctggagctat gaaccgactc
     4561 ccttccatgg cgtatatgtc cgggacttcc ttataaaggc ggcgtcccgc ggagtaagcc
     4621 tttttgacgg gaataactac acaggaaagt cgatagacat aaagccgggc aaatatacca
     4681 gcgctgatct caaggcagcc ggtatggacc cggcttccat caaatcggta aagctgaaca
     4741 acgaccacag cctggcatac aagctgacac tgttctcgaa cgacgatttc cgcggaagca
     4801 gccaaacctg catttacaac attccggacc ttcaggcatt gttagctgat aaaaccgtaa
     4861 aatcactgaa acttgaaact gtgaagcttg aaaacatcaa taagggcagc agtgtcgaat
     4921 gcagcagcgg caatgaccaa agcggtgctg tcaccgacgg ggatgagacg accaggtgga
     4981 tagtcgatga agaaggcccc aaatgggtca taaccgacct gggaacagtc tgtacgatcc
     5041 acaggatcat acttaagcac gcatccggca agaacctcaa caagcataac aataaccagg
     5101 atttcaaagt aagcataagc gctgacggca caagctggac agaggtatat gcagccacag
     5161 gcaataagga ttacgaaagc gtgatcgatt tcgagccttc cgccgcacgg tacgtaaaag
     5221 tggacataat gaagggcagc cagattccgc cgcacacacg cgcaaccata agcgagatcg
     5281 aggtttacgg cttaaaagga tcggagacag ccggtgaaag gtctgcagaa actcagggcg
     5341 gtgacggtaa cggaagcttc gccggaaatc cctggatatg gctcgctttt tccggtctgg
     5401 cttctatact tgttatagcg cttacggtat tatccaggcg gaaagagaga atacgaggga
     5461 ccgcatgaat gaaacagtac tgtaatgaac aggctgcagt atccgttaat gggtatgatg
     5521 cagtatgaaa agaattccgc ttaacacctg aaaaatcata tagtaagcaa agagtgcacc
     5581 gtccggatta aagggctgca gttatataaa ctacagcccc ttaattttaa gcatatcaga
     5641 aatcacaaaa tctctccgtc ataaggcccg gttcattgtt ccttttcgaa catgtccttt
     5701 cctactccgc acagcgggca aacccagtca tcaggaatat catcaaacgg tgtgcccggc
     5761 gcgatcccgc cgtcaggatc ccccacggcc ggatcatata cataaccgca ggcagtgcaa
     5821 acgtatctgt ccatagaatc aactccttaa aaatgatatc tctaattatg ttccccttca
     5881 attatatcaa taaacaatgt tttaaaaaag cggggcggta aatatcctgc ctgcttcaga
     5941 catgtttctt cctcccgccg gaaaccagga tgatcccgac gccgataagc agcaccggca
     6001 tcgcatatgg tttggtgttg aagctaagaa gcctgctcag cgagaatgag aagaaaaaca
     6061 ggaatgacag tccgctcagg atgccgatgg ggatcagcag ccctttttcc ctgtttccga
     6121 atatataaag ctcaaaaagt ccgaaagcta tcgagaatat gatcagcggc caggttatgt
     6181 cccagcctcc gaacagcatg ttgaactgga acaccaggcc gagcaccagc agtatcccgc
     6241 cgggcacgag caaccctgga ttccttctgc ttccggagaa ataaccataa tggaagagcg
     6301 ccccgggcag cagcaaaaac agcgatggcc agaattttgc caataatgct ccgggattga
     6361 tgatcctcca tacaggttcg aatatatcca catttcttat aaggaacagt ataccaagag
     6421 caacaagtat accgcccagc actctgccgg accttttcat tttaaaacct cccaggtaaa
     6481 taatcagaca ttgccgtctc tgttcatact ttctatataa ttataatgtc gcgccggcaa
     6541 aatgtctgat gattttggga gtgcagacga aaacatctca tttttccgcg gttccggctt
     6601 cagacctgtc cgtgagcagc aggccgctct tttcaagctt ccatatgacg aaccgtatgc
     6661 ctgatgctat aaggtccgcc atccgcatca ctacatggag ccttgtattc tgcaggatca
     6721 gaaaatccat gaagccgccg aaattgacga tcccggtca
//
LOCUS       DAOSCC010000136         6746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7266_length_6746_cov_4.806456, whole genome shotgun sequence.
ACCESSION   DAOSCC010000136 DAOSCC010000000
VERSION     DAOSCC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7266_length_6746_cov_4.806456"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLG72_07880"
     CDS             <1..598
                     /locus_tag="PLG72_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOJ80753.1"
                     /translation="DSEHYYTEKPTSELKECQFRYRIKNAELSLISVSGVFAFENRVD
                     KASELLIQAFRPSGRSVLDLGCGYGAVGLFIKALYKEQLVCLSDVNERAVEYSMLNAS
                     NNHLDVKVVKSDLFSAFGGISFADIVTNPPFAAGKCLLANLIKEAYEHLSPNGALWTV
                     AYHNKGGSSLKKIMLERFSNVEDVVKSGGIRVYKSVRN"
     gene            complement(728..1600)
                     /locus_tag="PLG72_07885"
     CDS             complement(728..1600)
                     /locus_tag="PLG72_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041734410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ80754.1"
                     /translation="MSVGKNTGKNIIEVKNASYVYRSNSDDRQDVLALDEVSLAVRQG
                     EFLAVLGRNGSGKSTLAKLMNALILPVSGTVIVDGIDTTNEEMLWDIRCTTGMVFQNP
                     DNQIVGTVVEEDVAFGPENLGVPPKEIRTRVDEALEMIGMSEFKKHAPHQLSGGQKQR
                     VAIAGILAMRPKCIVLDEATAMLDPVGRKEVMRILRKLNTGEGITIVHITHHMDEAGM
                     ADRVLVIDGGKCVMLGSPRDVFSDVKRIKSLGLDVPQVTELMYELNKSGFDFPTDILT
                     VDEAVKRLQEYLGE"
     gene            complement(1828..2361)
                     /locus_tag="PLG72_07890"
     CDS             complement(1828..2361)
                     /locus_tag="PLG72_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HOJ80755.1"
                     /translation="MPTLRKLGRATDQRRAMLKSLVTALLHKGRIETTEARAKEVQAI
                     AEKLIAMAIKEDGNFTSKQIKVSAAKTDSKGRKITKSVTSKNGRKYEVVDREVKTDMV
                     TVDNPSRLSARRRAMKWIYRVKDENGEYINVVNKLFDEIGPRYKGVNGGYTRIYKLGP
                     RRGDGAEVVILELTKQQ"
     gene            complement(2390..3337)
                     /locus_tag="PLG72_07895"
     CDS             complement(2390..3337)
                     /locus_tag="PLG72_07895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ80756.1"
                     /translation="MIEIEKPRIECVESLEDNTYGKFVVEPLERGYGITLGNSLRRIL
                     LSSLPGVSVTSIKIDGVLHEFSTVPGVIEDVTEIILNIKNLSMKLHSDGPKIIYIDAE
                     GEGPITAGDIKADSDVEILNPDLHICTLNGDSRFYMEMVINKGRGYVPAEKNKQPGQP
                     IGIIPVDSIYTPVRRVNYTVENTRVGQVTDYDKLTLEVWTNGSLKPDEAISLGAKIMS
                     EHLNLFIDLSDQAKHTEIMVEKEETKKEKVLEMTIEELDLSVRSYNCLKRAGINTVED
                     LISRTEEDMMKVRNLGRKSLEEVLQKLHALGLSLAPSEE"
     gene            complement(3555..4181)
                     /gene="rpsD"
                     /locus_tag="PLG72_07900"
     CDS             complement(3555..4181)
                     /gene="rpsD"
                     /locus_tag="PLG72_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ80757.1"
                     /translation="MARYTESVCRLCRREGEKLFLKGERCYTNKCSVGRRAYAPGQHG
                     QQRKKMSEYGMQLREKQKARRFYGILESQFRKYFEMAVKRKGVTGENLLQILESRLDN
                     VVYRLGLATSRPEARQLVRHGHFAVNGKKVNIPSLLLSPGDVITVRDNFRNSDKLKTI
                     LDIAGGKVVPEWLEFDSENLTGKVVRQPAREEIDLPIREHLIVELYSK"
     gene            complement(4201..4602)
                     /gene="rpsK"
                     /locus_tag="PLG72_07905"
     CDS             complement(4201..4602)
                     /gene="rpsK"
                     /locus_tag="PLG72_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOJ80758.1"
                     /translation="MAAKATGKRTTRKRKERKNIERGAAHIRSTFNNTIVTITDTAGN
                     AISWASAGGLGFRGSRKSTPFAAQMAAETAAKAAMEHGLKTVEVYVKGPGSGREAAIR
                     AIQAAGLDVSLIKDETPIPHNGCRPPKRRRV"
     gene            complement(4624..4998)
                     /gene="rpsM"
                     /locus_tag="PLG72_07910"
     CDS             complement(4624..4998)
                     /gene="rpsM"
                     /locus_tag="PLG72_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOJ80759.1"
                     /translation="MARIAGVDLPREKRVEIGLTYIYGLGRSKAKEILEKTAINPDTR
                     VRDLTDDEISRLRELIDKEYKVEGDLRREVALNIKRLIEIGCYRGRRHRAGLPVRGQR
                     TKTNARTRKGPKKTVGVQRKKS"
     gene            complement(5343..5456)
                     /gene="rpmJ"
                     /locus_tag="PLG72_07915"
     CDS             complement(5343..5456)
                     /gene="rpmJ"
                     /locus_tag="PLG72_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOJ80760.1"
                     /translation="MKVKPSVKAICEKCKIIRRKGKVMVICSNPKHKQKQG"
     gene            complement(5554..5772)
                     /gene="infA"
                     /locus_tag="PLG72_07920"
     CDS             complement(5554..5772)
                     /gene="infA"
                     /locus_tag="PLG72_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ80761.1"
                     /translation="MSKEDVIEVEGRIVEALPNAMFQVELDNGHRVLAHISGKLRMNF
                     IRILPGDRVTLELSPYDLTRGRITWRAK"
     gene            complement(5789..6052)
                     /locus_tag="PLG72_07925"
     CDS             complement(5789..6052)
                     /locus_tag="PLG72_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOJ80762.1"
                     /translation="MNAAVGQIVVSRAGRDAGRAFVVAKVIDDLYVEICDGDLRKVEK
                     PKRKKIKHLNITDKFAEGLAEKLKNGDRITNAEIRKALADFVS"
     gene            complement(6049..>6746)
                     /gene="map"
                     /locus_tag="PLG72_07930"
     CDS             complement(6049..>6746)
                     /gene="map"
                     /locus_tag="PLG72_07930"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ80763.1"
                     /translation="AVRPGITTLELDRIAESYITRLKAIPLFKGVPCPIDGGSDFPGT
                     ICTSVNDEVIHGIPGLRELKDGDIISIDMGASFDGYCADAARTFPVGNISDGARRLIE
                     VTEQCFYEGLKYAVKGNRIVDISRAVQRHAENNGFSVVRDYVGHGIGRELWEAPQIPN
                     YVTRERGPRIEPGMTLAIEPMINQGVCNVRILNDRWTVVTADGKLSAHYENTIAVTDG
                     EPVIMTKLRGEDP"
BASE COUNT         1478 a         1872 c         1389 g         2007 t
ORIGIN      
        1 tgatagcgaa cattattaca cggaaaaacc aacttccgaa ttgaaagaat gccagttcag
       61 atacaggata aaaaatgcag aactgtccct catttctgtc agcggggttt tcgcgttcga
      121 aaacagggtc gataaggcat ccgaattgct tatccaggct ttcagaccgt cggggcgttc
      181 ggtgctggac ctgggatgcg gctacggcgc tgtagggctg ttcataaagg cgttatacaa
      241 agagcaattg gtctgtcttt cagatgtgaa tgaaagagcg gtagaatatt ccatgctgaa
      301 tgcgtcaaac aaccatcttg atgtaaaagt cgtaaagagc gatcttttct cagctttcgg
      361 cgggataagc ttcgccgata tcgttacaaa tccgcccttc gccgccggca aatgtctgct
      421 ggcaaacctt atcaaagagg cttatgaaca cctgagccca aatggcgcgc tttggacggt
      481 ggcataccac aacaagggcg ggtcttcact gaagaaaata atgctggagc gcttttctaa
      541 tgttgaagac gtggtaaaaa gcggaggcat cagggtgtat aaatcggtac gcaattgagt
      601 acacaactta ggattaagcc ggtattcagg ctttcgtgta atattttcac ggttaatacg
      661 ataatccggt ccaaatcctg tcatagacgg atctgaaacc gtcaacgctt taccttaagc
      721 ttacagctca ctctcccaga tattcctgca gccgcttcac tgcctcatcg actgtcagga
      781 tatccgtggg gaaatcgaaa cctgatttgt tcagttcata cataagctct gtgacttgcg
      841 gcacatcaag ccccaggctc ttgatccttt ttacatcact gaacacatcc ctcggagagc
      901 caagcatgac gcacttgccc ccgtctatga cgagcaccct gtctgccatg cccgcctcat
      961 ccatatgatg ggttatatgg actatggtga tcccttcccc ggtattcagt ttgcgcagta
     1021 tgcgcatgac ttcctttctt ccgacgggat cgagcattgc cgttgcttcg tccagcacga
     1081 tgcatttggg tctcatggcc agtatgcccg ctatcgcgac cctctgcttc tgcccgcctg
     1141 acaactggtg cggcgcatgc ttcttaaatt ccgacatgcc gatcatttca agggcctcat
     1201 ctaccctggt ccttatctcc ttcggcggca ctcccaggtt ctccgggccg aaagccacat
     1261 cttcctccac taccgtgccg acgatctggt tgtcagggtt ctggaacacc atacctgtag
     1321 tgcacctgat atcccagagc atctcttcat tggtcgtatc tatgccgtcg actataacag
     1381 tccctgacac cggcaggatc aatgcattca tcagtttggc cagcgtcgac ttgccggagc
     1441 cgttcctgcc gagcacagca aggaattccc cctgcctgac ggccaggctg acttcgtcaa
     1501 gcgccagtac atcctgcctg tcatcgctgt tggacctgta gacatagctt gcatttttta
     1561 cttcgattat gttcttacca gtgtttttgc caactgacaa cgcttaaccc tctttctttt
     1621 cccggctcat caaaatatcc atgtacgctt agtggtcagc attccacatg atcagcaact
     1681 gttcagcgga taagtaaatc aggcaacacg gttctcataa cccaggcgga tcatccatcc
     1741 gacgagcata acacgaataa tccgcttgaa acaaaatcag ggattggcct tcgttccggc
     1801 aaatccctgg catggctcac ataaatctta ctgctgcttc gtcagttcga ggatgacgac
     1861 ttccgctccg tcgccccttc tcggtccgag cttgtagatc ctggtgtatc cgccgttaac
     1921 acccttgtac ctcgggccga tctcgtcaaa gagcttgttg acgacattga tatattcgcc
     1981 gttctcgtcc ttgaccctgt atatccactt cattgccctt ctcctggcac tcagccttga
     2041 aggattatcg accgttacca tatcagtttt gacttccctg tcaacgacct cgtacttcct
     2101 gccgttcttc gaagttacgc tcttggtgat ctttctgccc ttgctgtccg ttttggcagc
     2161 acttaccttg atctgcttcg atgtaaaatt cccatcttct tttatagcca tggcgatcag
     2221 cttttcagca attgcctgta cttcctttgc tctggcttcg gtcgtttcga tcctgccttt
     2281 atggagcaat gcggtaacga gacttttcaa cattgccctt ctttgatcgg tagcacgtcc
     2341 aagctttcta agtgtaggca tttctctctt acctccctga attccaattt cattcttcac
     2401 tcggagcaag cgacaacccc aaagcatgca gcttctggag tacttcttcg agcgactttc
     2461 ttcccagatt tctcactttc atcatatctt cttccgtcct gctgatcaaa tcctcgaccg
     2521 tgtttatacc tgctctcttc aggcagttgt aagatctgac ggacagatcg agttcttcga
     2581 tggtcatttc aagaactttc tctttctttg tctcttcttt ctcgaccatt atttcagtat
     2641 gctttgcctg atccgaaagg tctatgaaca ggttcagatg ctcgctcatt atcttggctc
     2701 caaggcttat tgcctcgtca ggcttcaggc ttccgttggt ccagacctcc agcgtgagct
     2761 tgtcataatc agtgacctga cctacacgcg tattctccac cgtgtaattg acccttctca
     2821 caggtgtata tatggaatca acaggaatta tgccgatagg ctggccgggc tgcttgttct
     2881 tttcagccgg cacatagcct ctgcccttgt tgataaccat ttccatgtaa aacctgctgt
     2941 caccattcag cgtgcatata tgcagatccg gattcaggat ctccacatca gagtcggctt
     3001 ttatatctcc tgctgtgatg gggccttcac cctcggcgtc gatgtatatt atcttcggcc
     3061 cgtcgctgtg gagcttcatg gaaagattct tgatgttgag tattatctcg gtgacatcct
     3121 cgatgactcc tggcactgtt gaaaactcat ggagcactcc gtcgatcttg atggatgtca
     3181 ctgaaactcc cggcagggac gaaagcaata tacgcctgag cgagttgccc agtgttatgc
     3241 cgtaccctct ctcaagaggc tcgactacaa atttaccata agtattgtct tctaaagact
     3301 caacacattc gattctcggc ttttctattt ctatcaacaa aaaccctcct ttggcttgca
     3361 ttttgatttt tatgagggca actgattcgt aatccttttg tcatatccag tactctgttc
     3421 gcaataccct gatgcatgta atgcaggaat ggtcagttgc ttttcaggct ttccggagca
     3481 ccggcaccag cgatgctcct ttcagccgtt tgaactggtt taatctatgg gtatatatca
     3541 acagcatcaa cagattactt ggagtacaac tcaacaatga gatgttctct gatcggaaga
     3601 tcgatttctt ctcttgccgg ctgtcttacg actttaccgg taagattctc actgtcgaat
     3661 tcgagccatt ccggtactac cttcccgcct gcgatatcga ggatagtttt cagtttatcc
     3721 gaatttctga agttgtcacg taccgtgatg acatcccccg gagacaaaag caatgaaggt
     3781 atgttgacct ttttgccgtt cactgcgaag tggccatgcc ttacaagctg ccttgcctca
     3841 ggcctggatg tagcaagtcc aagtctgtaa accacattgt caagtctgct ttcaagtatc
     3901 tggagaaggt tctcgccggt gactcctttt ctcttgacag ccatttcgaa gtatttcctg
     3961 aactggctct caagtatccc ataaaacctt cttgctttct gtttctcacg cagctgcatg
     4021 ccatactcgg acatcttctt cctctgctgt ccgtgctgtc ccggagcata cgcccttctg
     4081 ccaacagagc atttattggt atagcatctt tcgcctttca gaaaaagctt ctcgccttcc
     4141 ctgcggcaaa gtctgcaaac tgattccgtg taccttgcca tctgtcttta cacctccaaa
     4201 tcacactctt cttctcttgg gcggcctgca tccgttgtgc ggaataggcg tctcgtcctt
     4261 gatcaggctg acatcgagcc ctgccgcctg tattgctctt attgcggctt ctcttccgga
     4321 acccggtccc ttgacgtaca cctcaacagt cttgaggcca tgctccattg ctgctttcgc
     4381 agcagtctcc gcagccatct gcgctgcgaa cggagtactc tttcttgaac ccctaaagcc
     4441 caatccgcca gcacttgccc atgagatggc atttcctgct gtgtcagtta tagtaactat
     4501 agtattattg aacgtagaac ggatatgtgc cgcaccgcgt tcaatattct tgcgctcttt
     4561 tcttttcctg gtagttctct tcccggtagc tttagctgcc atgcgttaat caacctccta
     4621 acattacgat ttcttacgct gcactccgac agtcttcttg gggccttttc tagtccttgc
     4681 gttggtcttt gtcctctggc ctctgaccgg caaaccggcc ctgtgccttc tgcccctgta
     4741 gcagcctatc tctatcagcc tcttgatgtt cagagcaacc tctctcctga ggtcaccttc
     4801 caccttgtat tccttgtcta tcaattccct gagcctgctg atctcgtcat cggtcaggtc
     4861 tctgactctc gtatcagggt tgatagcggt cttttccagg atctctttcg ctttgcttct
     4921 gcccaaacca tagatgtaag taagtccgat ctctacccgt ttttccctgg gcaggtctac
     4981 tccggcaata cgtgccatct tgtcaaacac ctccaaataa tcttaccgtg taacaacctt
     5041 attaactaag aatttcctgc agggtcagat acattatggc acatatgtca acataaataa
     5101 cataccttaa tatattgacc cggtatatat gatcaacacc cggatcatgt gcgtttaggg
     5161 cgcacaagca gccgcttccg ggatggtcaa ttcaactttg ttccagggat ggataattga
     5221 taattgaccg caagtcaagc ttaaattata tcacacaccg cccgaataaa caagcttgca
     5281 tgcgcaaatt ccaattatca ggtatccgtc tgctccgaaa cgtgtccggt gcatacagaa
     5341 catcaaccct gtttttgttt atgcttcgga tttgagcaaa taaccattac tttgcctttt
     5401 cttctgatta ttttgcattt ttcacatata gctttgactg aaggtttaac tttcatttta
     5461 tgcactccca tccggttaga atatagattt attacaatat tgtctttgat ctgccgtccc
     5521 gttcgggaca agcaggactt gaaaccatgt cctctatttg gctctccagg ttatccttcc
     5581 gcgggtgaga tcgtacggcg acagttccag tgtcacccgg tcccccggca ggatccttat
     5641 gaaattcatc ctgagcttgc ctgaaatatg ggcaagcacc ctgtggccgt tgtccagttc
     5701 cacctgaaac atcgcattgg gcaatgcttc aacgattctt ccctcaacct cgattacgtc
     5761 ttccttagac aaaactcaaa ccctcctttt aactgacaaa atctgcaaga gctttcctga
     5821 tttcagcatt tgtgatcctg tctccgttct tcagcttctc tgcaagtcct tccgcaaatt
     5881 tgtctgttat gttcagatgc tttattttct ttctcttcgg tttctccact ttacgcagat
     5941 cgccgtcgca tatctcaaca tacagatcat cgatcacttt cgccactacg aatgctctcc
     6001 cggcatccct gccggcgcgg gatacaacta tctgacctac agcagcattc aagggtcctc
     6061 acccctgagt tttgtcatga taaccggctc gccgtctgta acggctatgg tgttctcata
     6121 atgagcggac agttttccgt cagccgttac cacggtccat ctatcattga ggattcgcac
     6181 attacaaact ccctggttga tcatcggttc tatcgccagc gtcattcccg gctcaatcct
     6241 gggtcctctt tcccttgtga cataattggg tatctgggga gcttcccaca actccctgcc
     6301 tatgccatga cccacataat cgcgcactac cgaaaatcca ttgttctcag cgtgtctttg
     6361 tacggctctg gagatatcaa cgatccggtt gcctttgaca gcgtatttca gtccttcata
     6421 aaagcattgc tctgttactt ctatcagcct gcgggcgccg tccgatatgt ttccgacagg
     6481 gaaagtcctt gcagcgtcgg cacaataacc gtcaaagctg gctcccatat cgatactaat
     6541 aatatctcca tcttttaact ctcttaaacc cgggatcccg tgtatgacct catcgttgac
     6601 cgatgtacag atcgtaccgg ggaagtccga tccgccgtca atggggcacg gcacaccctt
     6661 gaacaaaggt atcgctttca gtcgggttat atagctttca gctatcctgt caagttccaa
     6721 tgttgttatg ccgggtctga ccgcct
//
LOCUS       DAOSCC010000137         6721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7294_length_6721_cov_3.241374, whole genome shotgun sequence.
ACCESSION   DAOSCC010000137 DAOSCC010000000
VERSION     DAOSCC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7294_length_6721_cov_3.241374"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="PLG72_07935"
     CDS             <1..2100
                     /locus_tag="PLG72_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ80764.1"
                     /translation="GKEDEARAYQGDDNFTDADQVKWPEGRNILAYLKDNPQYGFIGY
                     PDGRFGVDDKLLPRQLYKVLLTAMGYKEGEDFSYADVIEFAQARGFTGLSPDNGPVKI
                     AQMISAVVEALGLPTADGQQTLAGKFGLAAFAQFGSPVIDGEVDEIWSKATPIVPRYS
                     TTTENTTAEFRAMWDDKALYILAQVKDENLSKDSVNPYEQDSLEIFLDENNDKTREYG
                     ADDLQFRINYANEETADKGDLTRLYSQAKVVDGGYVIEARIAWDKLVPANGKVVGLEL
                     QINDAKGAARVGTINVFDGTNNAWQNTALFGNLILEGKAPGSLTGKDPYTLLSFIKSI
                     YRKYNKDDYKNFDIVEEAIIAAEAVAADKDSTQEDYDEQYANLQKALSQLEYTEEAAK
                     IKRFAEMPSEYKDVCTSGGTIETLSYDVKISVKDEDGTETEKTDTKYLNVYLPYGYDP
                     DDKAVKYNVLYLMHGGGENWQLLFGGPGEERELKRILDNMIARGDIDPLIVVTPSFYN
                     SDGSFDFNAFAGEELLNQIIPLVEGKYNTYAAAAAAASDGAITPDVIRATRGHRAFGG
                     FSMGSVCTWAVLANCIDYVKHYIPLSGESWTFEGNTPAERAQAMAELLKAHNMGSRDF
                     YILGATGSADIAYPNMKPWMDALEEIGDPFIFNRDLTVGNCYFMVGENGTHAWNFVNQ
                     YLYNILPDLSYLFSSES"
     gene            2545..2913
                     /locus_tag="PLG72_07940"
     CDS             2545..2913
                     /locus_tag="PLG72_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80765.1"
                     /translation="MSNQVTIINRDLIDGMEFRVRNLTDDKEIALEDITGRFTIRTGR
                     RSGIYSVRKFRLDSRGDIELEVSGSSSESDDVKVFISSKMIPAAGGFEVFGRMFLVVL
                     VLMVLQIVIIRILKRLGVAR"
     gene            complement(3130..5097)
                     /locus_tag="PLG72_07945"
     CDS             complement(3130..5097)
                     /locus_tag="PLG72_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOJ80766.1"
                     /translation="MGVINVRGNSIVFQKRDEIAILEPWGKDCLRFRSTPNSAIADEN
                     WNLLAQPEAERSINTDEEKATIVNGKISAEIFKNGKVVYYKNGKEILAERSEMAFYSR
                     YREYRSLGADNHRATVIFEPNEHEHFYGLGQEQNNCFDLKGSTSQLLHKNTKSSIPFV
                     YSSRGYGFLWNNPSVGRCELTHNHTLWEANSTKQVDYVVIAGDTPAEVMSKYADLTGH
                     APKFPAWASGFWQCKLRYTTQDELLEVAREYKRRGIPISAVIIDFFHWTEQGDLKMDP
                     RYWPDPKKMCEELDEMGIRVIASVWPTINPKSENYAYMNERNMLVRTERGQYGIFDFH
                     GLQTYFDAMNPETREFVWSKIYENYYKNGIKTFWLDQAEPEFHPNQYDNLRFYRGNGE
                     EVALLYPYHYSQLFYEGLKSAGESEIISLTRAAWIGSQKFGALVWSGDIPSTFESLRM
                     SVKTGLNMAMSGIPWWNSDIGGFWGGDPESEYFRELIVRWFQFGVFCPVMRLHGDRKR
                     AKNHPLRDSETLQPSGGDNEIWSFGEKVYPILKELIELRERLRPYIHKYMDIASETGA
                     PIMRPMFYEYPDDEVCYTLGDQYMFGSDILFAPIVNQGQTDRRVYLPAGTWVNVNDKQ
                     EYEGGKFVDAHAELHQFIAFVKKGAEVLSVF"
     gene            complement(5298..>6721)
                     /locus_tag="PLG72_07950"
     CDS             complement(5298..>6721)
                     /locus_tag="PLG72_07950"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ80767.1"
                     /translation="IQDALDLIEFANGGPDTVWGKKRAELGHPEPFNLKYLGIGNEEV
                     GEDFFVRYELIHKRVKEKYPEIKLINTAGPNAAGSEFERGWASAKENGSDYVDEHYYQ
                     APEWFMANMHRYDSYPENGPKVFLGEYASWGSTYRNALVEAAYMIGLEKAPAVGLACY
                     APLLCNTGYQKWYPDLIYFNNHQAFGSACYYVQKLFMNHQGDDLLAVSAEHLPEPEKT
                     YEPVRGKIVVVPTKATVQYKDFELVDPHTGTVSKLPDVSAGGASLIPVAETEGDFILR
                     FTAVKLDESNQDSPFGDRGFEIRFGWQNEDNYLSWKVGAWQNQDSFLRSVVNGKSSDL
                     TQSLFTVKEDVEYRCELRVTQRKMTGIINGSVINEAEDSLPVIDPLYYTASLDHESGD
                     VIVKIVNLSEHERPVTISLEQYGAQRLNGTLYEMKGYAPDDENSFDDPKKVSYTERPF
                     RAEKPEFEHVFGPRSLSVFRFHA"
BASE COUNT         1627 a         1637 c         1711 g         1746 t
ORIGIN      
        1 ggaaaagagg atgaggccag ggcatatcaa ggcgatgata acttcaccga tgccgaccag
       61 gtgaaatggc cggaaggaag gaatatcctg gcatatctca aggataaccc gcagtacggg
      121 tttatcggat acccggacgg caggttcgga gtcgacgata agttacttcc ccggcagctt
      181 tataaagtat tgcttacggc aatgggatat aaagaaggag aagacttcag ttacgcagac
      241 gtaatagagt ttgcgcaggc caggggcttc accgggctca gtccagataa tggccctgta
      301 aaaatagccc aaatgatatc tgcggttgta gaggcactgg gacttcccac ggccgacggg
      361 cagcaaaccc ttgccggcaa atttggcctt gctgcattcg cgcagttcgg ttctcccgtg
      421 atcgacgggg aagtggatga aatatggagc aaagccacac ctattgttcc gagatactcc
      481 acaacgaccg aaaacactac agctgagttc agggccatgt gggatgacaa ggccttgtac
      541 atattggccc aggtaaagga tgaaaacttg tcaaaagatt ccgtcaaccc atacgagcag
      601 gattcgttgg agatcttcct cgacgagaat aacgacaaga caagggaata tggagcggat
      661 gacctgcagt tccgtatcaa ctatgcaaat gaagaaactg ccgacaaggg cgatctgaca
      721 cgtttgtatt cgcaggcgaa ggtcgtcgac ggcggctatg ttatcgaggc aagaatagca
      781 tgggacaaat tggtgccggc aaatggtaag gtcgttggcc ttgagctgca gatcaatgat
      841 gcgaagggcg cggcccgtgt tggtacgatc aatgtatttg acggtacgaa taatgcatgg
      901 cagaacacgg cattgtttgg caaccttatc ctggaaggga aggctcccgg ttcgttaacg
      961 ggaaaagatc cttacacgct tctgagcttt atcaagagca tatacaggaa atataacaag
     1021 gatgattaca agaacttcga tatcgtagaa gaagccatta ttgcagcaga agctgtcgca
     1081 gctgacaagg attccacgca ggaggattat gatgaacagt atgccaattt gcagaaagcg
     1141 ttgagccagc tggaatatac tgaagaagct gcgaagatca agcgctttgc agaaatgcct
     1201 tccgagtaca aggatgtttg tacatccggc gggactattg agactttgtc atatgacgtt
     1261 aagatcagcg ttaaggacga ggatggcacg gaaacagaaa aaactgatac caagtacctc
     1321 aatgtatacc tgccgtacgg atacgacccg gatgacaagg ctgtgaagta caatgtgctc
     1381 tacctgatgc atggcggcgg cgagaactgg cagctgctct tcggaggccc gggggaagag
     1441 cgtgaattga agaggatcct tgacaacatg atcgccaggg gcgacattga tccgttgatc
     1501 gtcgttacgc cgagtttcta caatagtgac ggatcatttg actttaacgc gtttgccggc
     1561 gaagaattgc ttaaccaaat aattcctctt gtagaaggta aatacaatac gtatgcggca
     1621 gcggccgcag cagcatcgga cggtgcaatt actcctgatg tcatcagggc tacgcgcggt
     1681 cacagggcat tcggcggatt ctcgatgggc tccgtttgca catgggctgt tctggccaat
     1741 tgcatcgatt acgttaagca ctatataccg ctgagcgggg aaagctggac attcgaaggc
     1801 aacactccgg cagagagggc ccaggcgatg gcggaactcc ttaaggccca caacatgggc
     1861 agccgcgatt tctatatttt aggtgctaca ggcagcgcgg atatcgctta tccgaacatg
     1921 aagccgtgga tggatgcact ggaggagatc ggtgatccgt tcatattcaa cagggacctt
     1981 accgtgggca actgctactt catggtagga gaaaacggga cacacgcatg gaactttgtc
     2041 aaccagtact tatacaacat tctgcccgac ttaagctact tgttcagttc tgaatcctaa
     2101 gtccctggca agacagctgc aggctggtac gccaaaaagc aggagggaga ttaacgtctc
     2161 tcctgcgctt ttatgggcgg cctgccatgc caggacgatg aatgccggaa ggccgcagat
     2221 ggactcagtt ggactgtttt tctgcgaggt ggatgcaagc cgtgcaaaat gatataatta
     2281 ccagtggaat aggtgaagga gtgctgctgc aaggtaaatt cgtccggtag cggggggttg
     2341 tccatgcgta atgctaagaa aattgtttcc gctataatgg tcgtaattgc catatcatat
     2401 attgtcatat ctttatggga ttatagcggg agaaaaaaag aattttttga tgacccggat
     2461 aaaatttcac cacccggcag agcagtgtac agtgatttgg agaaaggcac ttactatatc
     2521 ttcgttttaa gcaggcatgc tcttttgtcc aaccaggtca cgataatcaa tagggacctg
     2581 atagacggga tggaattcag ggtcaggaac ttaactgatg acaaggaaat cgcgctggag
     2641 gatattaccg gccgattcac gatacgtacc ggaagaagat caggtatata ttcggtacga
     2701 aaattccggt tggacagtcg gggagacatt gaactggaag tttcgggcag cagttctgaa
     2761 agcgatgacg tcaaggtgtt tataagcagt aaaatgatac ccgctgccgg aggatttgaa
     2821 gtcttcggcc gtatgtttct tgtggtactg gtcctgatgg tattacaaat cgttataata
     2881 cgcatattga agcggttggg tgttgctcgc tgatgcaaag aatgaaaata agccggagtg
     2941 gatccggcag gcaacagctg cattgaggcc ggcagtttcg gacaggcaat accgtcagct
     3001 tactgatatt ttgaggcagc aaaaacagta caggcacacc aggcagtcat acgtctgcaa
     3061 taacaggatt cgtgactatc tggcgtgcct gccatgttat atgctatatt ccatactccc
     3121 tgccatattc taaaagaccg aaaggacctc ggcgcctttc tttacgaagg ctatgaactg
     3181 atgcagttcc gcgtgggcat ctacaaattt tccgccttca tattcctgct tatcgttgac
     3241 attgacccat gtccctgccg gaaggtatac cctgcggtct gtctgcccct ggttgacgat
     3301 tggggcgaat aggatatcgc tgccgaacat atactggtct cccagggtat agcagacttc
     3361 atcatccgga tattcataga acatcggacg cattatcgga gcccctgtct cgcttgcgat
     3421 atccatatac ttatggatat atggacgcag cctttctctc agttcgatca attccttcaa
     3481 tatgggatac actttttctc caaagctcca gatctcgttg tctccgccgc tcggctgcag
     3541 ggtttcggaa tcgcgcagcg gatggttttt ggctcttttc ctgtcgccgt gcagtctcat
     3601 gacggggcag aatacaccga actggaacca gcgcacgatc aattcccgga aatactcact
     3661 ttccgggtcg ccgccccaga agccgcctat atcactgttc caccagggta tcccgctcat
     3721 tgccatgttc agtccggtct tgacactcat cctgagggat tcgaaggtgg acgggatgtc
     3781 tccggaccat accagcgcgc cgaatttctg gcttccgatc catgcagccc gggtcagtga
     3841 tattatctcg ctttcaccgg cacttttgag tccttcgtaa aagagctgag aataatgata
     3901 gggatacagc agcgctactt cttctccgtt tcccctgtaa aaacgcagat tatcgtattg
     3961 gttcggatgg aattcgggtt ctgcctggtc gagccagaaa gtctttatcc cgtttttata
     4021 ataattttca tagattttgg accatacgaa ttccctggtt tcgggattca tcgcatcaaa
     4081 gtatgtctgg agtccatgga agtcaaatat cccgtactgg cctctttctg tccttacaag
     4141 catattgcgt tcgttcatgt atgcatagtt ttcgcttttg ggatttatcg taggccatac
     4201 cgaagcgatc accctgatac ccatttcatc cagttcctcg cacattttct tcgggtcggg
     4261 ccaatacctg ggatccatct tcagatctcc ctgctccgtc cagtggaaaa agtcgattat
     4321 tacagcagag atcggtatcc cgcgcctttt atactctctg gccacctcga gcagttcatc
     4381 ctgggtcgta tatctaagct tgcactgcca gaatcctgaa gcccatgccg ggaacttcgg
     4441 cgcatggccc gtgagatctg cgtattttga catgacctcc gccggggtat ctcccgctat
     4501 aacgacataa tccacctgct tcgtggaatt cgcctcccac agcgtgtgat tgtgggtcag
     4561 ttcgcatcgc ccgacggacg gattgttcca gaggaacccg taaccccttg aggaatatac
     4621 gaaaggtatg gaggatttcg tgttcttgtg caaaagctgg cttgttgagc ctttcagatc
     4681 gaagcagttg ttctgctcct gtcccaggcc gtagaaatgc tcatgctcgt ttggctcaaa
     4741 tattactgtt gcccggtggt tatcagcgcc aagcgaccga tactccctgt acctgctgta
     4801 aaatgccatt tctgagcgtt cggcgagtat ttccttcccg tttttataat agacgacctt
     4861 tccgtttttg aatatttcag ccgatatttt gccgtttact atggttgcct tttcttcatc
     4921 ggtatttata ctgcgctctg cttcaggctg ggcaagaaga ttccagtttt catccgctat
     4981 tgcggaattg ggggtcgacc tgaaacgcag gcagtctttt ccccatggtt caagtatcgc
     5041 tatttcgtcc cttttttgaa atacgatact gtttcccctt acatttataa cacccatatt
     5101 ttcctccttc cctgtctgca cgacagcttc acgggactga tattagtgga aactcctgtt
     5161 acaattacac cataaattct gtaaaattgc actattctga ctgcaaatgg tcccttgtcc
     5221 cgggctgtga ccaaaaaggc acaggccggg acagggaatc ttttcctgtc tcgctcccgg
     5281 aaccctcggg tcacctgtca ggcgtgaaag cgaaagacgc tcagtgaacg gggtccaaat
     5341 acatgctcga attccggctt ttcagcccgg aacggtctct ccgtataaga aacctttttt
     5401 ggatcgtcaa agctgttctc atcatcagga gcatagccct tcatttcata taatgttccg
     5461 ttcagcctct gcgcgccata ctgttcaagc gagatggtca cgggcctctc atgttcagac
     5521 aggttgacta tctttacgat cacgtcgccg ctttcgtgat cgagactggc cgtataatac
     5581 agcggatcta tgaccggaag gctgtcttca gcctcattga tcacggatcc gttgattatt
     5641 ccggtcattt tgcgctgcgt cacccgaagc tcgcagcggt actccacatc ttcttttact
     5701 gtaaaaaggc tttgggtaag gtcggatgat ttcccgttta ccaccgagcg gaggaaagaa
     5761 tcctggttct gccatgctcc tactttccag cttaagtagt tatcttcatt ttgccagcca
     5821 aaccgtatct cgaagccgcg atcgccgaaa ggagagtcct ggttgctttc atccagcttc
     5881 actgccgtga aacgcaggat aaaatcacct tctgtctctg cgacagggat aagcgatgct
     5941 ccgccggctg acacatcggg aagcttgctg accgttcccg tgtgaggatc gacaagttca
     6001 aagtctttat actggacagt tgcttttgta ggtacgacga ctattttgcc cctgaccggt
     6061 tcgtacgttt tctcgggttc cggcaggtgc tccgcgctga cggcaagcag gtcgtccccc
     6121 tggtggttca tgaacagctt ctggacatag tagcaggcgg atccgaacgc ctggtggttg
     6181 ttgaaatata tcaggtcggg ataccatttc tggtagcctg tattacataa aaggggagcg
     6241 tagcaggcaa gcccgacggc aggagctttc tccagcccga tcatataggc ggcttccacc
     6301 agggcattgc ggtatgtact gccccaggat gcgtattcgc ccaaaaagac cttcggaccg
     6361 ttttccgggt agctgtcata gcggtgcatg ttggccatga accattccgg cgcctggtaa
     6421 taatgctcgt ccacgtaatc ggaaccgttt tcctttgcgg atgcccatcc tctttcaaat
     6481 tcgcttcctg cggcgtttgg tcccgccgta ttgatgagtt tgatttccgg gtatttttcc
     6541 tttacgcgtt tgtggatcag ctcatagcgt acgaaaaaat cttctccgac ttcttcgttc
     6601 ccgataccca ggtatttaag gttgaaaggc tccggatgcc cgagttcagc gcgttttttg
     6661 ccccagacgg tatccgggcc gccgtttgcg aattctatca ggtcgagcgc atcctggatc
     6721 c
//
LOCUS       DAOSCC010000138         6679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7358_length_6679_cov_4.742905, whole genome shotgun sequence.
ACCESSION   DAOSCC010000138 DAOSCC010000000
VERSION     DAOSCC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7358_length_6679_cov_4.742905"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLG72_07955"
     CDS             <1..487
                     /locus_tag="PLG72_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ80768.1"
                     /translation="PYLRRSLGVVFQDFRLLPNKTVYENVAFAMIITEALPKEIRRQV
                     PMALALVGLSRKANMYPHQLSGGEQQRVALARALVNNPALLIADEPTGNLDPETSWEI
                     MKLLSEINHRGTTVIVATHEKSIVDEMKKRVVAINKGVIVRDQEKGLYRDEDKNTQIF
                     Y"
     gene            456..1355
                     /gene="ftsX"
                     /locus_tag="PLG72_07960"
     CDS             456..1355
                     /gene="ftsX"
                     /locus_tag="PLG72_07960"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ80769.1"
                     /translation="MKIRTLRYFIKEGFVNSYRNKLMSLASILIVVASLIIFGVFVLI
                     SLNAKHNIEIWKEQPQLQAFCYEQLDETQIRRVEEIIKADDRILSYEKVTKEQAMERM
                     REKLGQEAAILDGYGADIFPVSFIIRLKDVSYSDEVVKDLEKVTGIEKVSYSHDTVEM
                     ITRVSHWFSLGCSVMIIIFLAASVFIIANTIKLTVFARRKEISIMKYIGATDWFIRWP
                     FVVEGVIISITGAILAFIISAYGYKAVVDKFSQDAFFAQTNLLSLLSIENVWSKIAVY
                     YAAIAVVVGASGSFLSMRRYLKV"
     gene            1396..2532
                     /locus_tag="PLG72_07965"
     CDS             1396..2532
                     /locus_tag="PLG72_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ80770.1"
                     /translation="MKRFTIAVLLIALTVCSVCPVMADELSEAKNKKAAADNRIADLT
                     KKKQTATKEKAALEQQKKSLTQQQNEEANAIEELERQISEAQEHLRQTELALAQATAE
                     YNQMDELVKTRLQVMYKNSGTTMLDTLLSSGSVVEFYERLHYMQVISENDNKLMQQLE
                     EARQDVELKKQMQEQAKALLEEKLAEREERLQQIKTSRAQVEGEISRTQKEIEQLEKE
                     IDKWLEESKKLASQIKNLSTKKKYVGGSMTWPCPSSQRVTSTFGMRKHPILRKYKMHT
                     GVDIAADKGAAIVAANSGTVIMAGYDSSGYGNMVVIDHGGGITTLYAHASKLLVKVGD
                     KVKAGQNIAKVGSTGLSTGNHLHFEVRVNGEPVDPMNGYLSKKK"
     gene            2664..3725
                     /locus_tag="PLG72_07970"
     CDS             2664..3725
                     /locus_tag="PLG72_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ80771.1"
                     /translation="MRRLFSIQQVAVIVAVTLAVSVGAAAFIFYSVHTAENRYMITFD
                     ANTVDYRNVVKFNRVMDILKNDFYQDVDENKMLEGAINGLAESLGDPYTVYFDKEHMD
                     AFLEKSRGSYVGIGVTVNLDDDGLLTVVEPMKGSPAMEAGMQQGDKIVKVDGKDVTSI
                     SDENMIISMIKGKENTRVKITVYRPLENRYVEFDIRRKRIKASNIRSEILDGNIGYIK
                     IVMFDSEIAKYFRNDLNSMIKKGIKGLIIDLRDNPGGSYEQVVEIADSLLPAGTIVYT
                     EDRNGRKEYRNSDKAYVDIPLAILINGNSASASEILAGAVKDHGRGILVGTRTFGKGL
                     VQELKLLEDGSGLKVTISR"
     gene            3979..4158
                     /locus_tag="PLG72_07975"
     CDS             3979..4158
                     /locus_tag="PLG72_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOJ80772.1"
                     /translation="MEKKTDPNEKLKFEIASELGLREKVMKYGWKSLSAKETGRIGGL
                     VSKRRKALQNGDQET"
     gene            4218..5054
                     /locus_tag="PLG72_07980"
     CDS             4218..5054
                     /locus_tag="PLG72_07980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ80773.1"
                     /translation="MGAAYHRMRLFCREFLWYHYGWASWKQSGVAAIYGDFAYYYDRL
                     MWDVDYSMWTDYVTDIFKWHGCEPKMIADLGCGTGSFCLEMSRRGFDMIGIDISAEML
                     SIAKQKAVSANADILFLNQDMTSFELYGTVDAVTCLMDSINYVTYKNDLKRLFRLVNN
                     YLNPGGIFVFDVNSPYKFERILSSNVFCESSEDVSYIWQNTYDKRSGLCRFDLTFFVK
                     EGKYYRRFDETHYERSYGTDELQQMLLAAGLTVHGIYGAFGSGKPGPKTERIFFACGK
                     HK"
     gene            5254..5457
                     /locus_tag="PLG72_07985"
     CDS             5254..5457
                     /locus_tag="PLG72_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOJ80774.1"
                     /translation="MQKGRVKWFNAEKGFGFIEREDGNDVFVHYTAIQMDGFKTLEEG
                     AEVEFEVVEGAKGPQAANVTKVM"
     gene            5588..6460
                     /gene="hslO"
                     /locus_tag="PLG72_07990"
     CDS             5588..6460
                     /gene="hslO"
                     /locus_tag="PLG72_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOJ80775.1"
                     /translation="MEDHIVRATAADGYVRAFAAVTTNLVQDAHKIHEASGVATVALG
                     RTLTAAAMMARMLKNDKDTMTIQIRGDGPLGLIVVVTDAFSNVRGYVHNPQVYLPLNP
                     AGKFDVSAAVGKNGYLNVIKDLGLREPYIGYVKLASGEIGEDIAYYFAASEQVPTAVS
                     VGVLVGPDQNVLAAGGYIIQLMPDAGEEIIDYLEEKVMFADSITCMLSDGMDAEAVLR
                     SLLGEKDLQIIDRLPCRYQCTCSRDRMERNLVSLGAVELGKMADEQHGAELQCHFCNK
                     KYYFTEEELRALIK"
BASE COUNT         1916 a         1419 c         1896 g         1448 t
ORIGIN      
        1 accgtacctc agacggagcc tcggggtcgt attccaggat ttcaggctgc tgccgaacaa
       61 gactgtctat gagaacgtcg cattcgcgat gataataacc gaggcgctgc ccaaggaaat
      121 aagaaggcag gtgcctatgg cactggcgct tgtcggactg agcagaaagg cgaacatgta
      181 tccgcaccag ctttcgggag gtgagcagca aagggtcgca ctggccaggg ctttggtcaa
      241 caaccctgcc ctgctgatag ctgatgagcc gacaggcaat cttgatccgg agacatcctg
      301 ggagataatg aaacttttga gcgagatcaa ccacaggggt acgaccgtca ttgtcgcgac
      361 acatgaaaaa tccattgtcg acgagatgaa aaaacgtgtc gtcgccatca acaagggcgt
      421 gatcgtaagg gatcaggaga aaggacttta cagggatgaa gataagaaca ctcagatatt
      481 ttattaagga aggtttcgta aattcataca ggaacaagct tatgtcgctg gcgtcgatcc
      541 tcattgtggt ggcgtcgctc atcatattcg gagtgtttgt gctgatatcg ctgaatgcga
      601 agcacaacat agaaatctgg aaggagcagc cgcagctcca ggcattttgt tacgagcagc
      661 tggacgagac gcagatacgc cgcgtggagg aaatcataaa ggccgatgac aggatccttt
      721 cctatgagaa agtgaccaag gagcaggcca tggagagaat gcgcgagaag ctcgggcagg
      781 aagcggccat actggacgga tacggagcgg atattttccc tgtatccttt ataattaggc
      841 tgaaggatgt gagctacagc gacgaggtcg taaaagatct tgaaaaagtg accggtatag
      901 aaaaggtcag ttattcccat gataccgttg agatgatcac gagagtatcc cattggttca
      961 gccttggatg cagcgtgatg ataataatat tcctggccgc atcggtgttc attattgcaa
     1021 acaccataaa actgaccgta ttcgcaaggc gcaaagaaat aagcataatg aaatatatag
     1081 gcgcgacgga ctggttcata cgctggcctt ttgttgtaga aggtgttata attagtatta
     1141 caggggcaat tttggcattt attatttctg cttacggata taaggccgtg gtcgacaagt
     1201 tttcacagga tgcgtttttt gcgcagacaa accttctgag cctgctgagc atcgaaaatg
     1261 tatggagcaa gatcgccgta tattatgcgg cgatagccgt cgttgtcggg gcgtcgggaa
     1321 gtttcctgtc gatgcgcagg tacctgaagg tgtgagaact taaaatccaa tgcatggggg
     1381 acaggatacg tgaggatgaa gagattcact atagcggttc tgcttatcgc actcacagtt
     1441 tgttctgtgt gtccggtaat ggccgacgaa ctttcagaag ctaaaaacaa gaaagcagca
     1501 gcggacaacc ggatagctga cctgacgaaa aagaaacaga cggcaacaaa agaaaaggcg
     1561 gcgctcgagc agcaaaagaa gagtttgaca cagcagcaga atgaagaagc gaatgctata
     1621 gaagagttag agaggcaaat ttccgaagca caggaacatc tgcggcagac cgagctcgcg
     1681 cttgcgcagg ctacagccga gtacaaccag atggatgaac tggtgaagac ccggctccag
     1741 gtcatgtata aaaattccgg tacgaccatg ctggataccc ttctttcatc aggaagcgtc
     1801 gtcgagtttt atgagagact gcattatatg caggttatat ccgagaatga caacaagctg
     1861 atgcagcaac tggaagaggc aaggcaggac gtggaactca agaagcaaat gcaggagcag
     1921 gccaaggcgc tccttgagga gaagcttgcg gagagagagg aacggctgca gcagatcaag
     1981 acttccaggg cgcaggtcga gggcgaaatt tctcggacac agaaagagat agagcagctt
     2041 gagaaagaga tagataaatg gctcgaagag tccaagaaac tggccagcca gatcaagaac
     2101 ctttccacga agaagaagta tgtcggcgga tcgatgacat ggccgtgccc aagctcacaa
     2161 agggtaacat cgacattcgg gatgaggaag cacccgatcc tcaggaaata caagatgcac
     2221 accggggtcg acatagccgc ggacaagggc gcagccatag tcgcggcaaa cagcggcact
     2281 gtaataatgg ccggttatga tagcagcggc tacggaaata tggtagtcat cgaccatggc
     2341 ggcggcatca ccactttgta tgcgcatgcc agcaagctgc tggtcaaggt gggggacaaa
     2401 gtcaaggcag gacagaacat agccaaggtt ggaagcacag gcctgtccac aggaaaccac
     2461 ctgcacttcg aagtcagggt caacggtgag ccggtggatc cgatgaacgg atatctgagc
     2521 aaaaagaagt aatatcagtt cgccgacagg acaggataat gcaaaaaaca taaataagcg
     2581 gtcaagcccg gttgaaaaac cgggtttatt tatttcagcg gccgcataaa gatataagga
     2641 atcaactttt gggaagtgtt tttatgcgca ggcttttttc aatccagcag gtcgctgtga
     2701 tcgtcgccgt gacgctggca gtttccgtcg gtgccgctgc gtttatcttt tattcggtcc
     2761 atacggcgga aaacaggtat atgataacct ttgacgccaa tactgttgac tacaggaatg
     2821 tggtcaaatt caaccgcgtc atggatatcc ttaaaaatga cttttaccag gatgtcgatg
     2881 aaaacaaaat gctcgaagga gcaataaacg gcctggcgga atcgctcgga gacccgtata
     2941 cggtgtactt cgacaaggag catatggatg ccttccttga gaagtcaagg ggcagttacg
     3001 taggaatagg ggtcactgtc aaccttgatg atgacggcct gctgaccgtc gtcgagccta
     3061 tgaagggttc tcccgcgatg gaagcgggca tgcagcaggg cgacaagatc gtgaaggtgg
     3121 acggcaagga tgtgacgtcg atcagcgatg agaacatgat catcagcatg atcaagggga
     3181 aggaaaacac ccgcgtgaag atcaccgtat acagacccct ggagaacagg tatgtcgaat
     3241 tcgatataag acgcaaacgc atcaaggcat cgaatatcag gagcgagata ctggacggga
     3301 atatagggta tataaagata gtcatgttcg acagcgagat agccaaatac ttcaggaatg
     3361 atctcaacag catgatcaaa aaaggcataa aagggcttat tatcgacctg agggacaatc
     3421 ccggcggatc ttacgagcag gtggtggaaa tagccgacag cctgctccct gccggcacga
     3481 tagtttatac ggaggacagg aacggcagga aggagtacag gaattccgac aaggcatatg
     3541 tcgacatccc gctggcgatc cttatcaacg gcaacagcgc cagcgcgtcc gaaatactcg
     3601 ctggcgcagt caaggaccac ggaaggggca tacttgtcgg taccaggaca ttcggcaagg
     3661 gactggtgca ggaactgaaa ctccttgagg acggttccgg tcttaaagtc accatttcac
     3721 gatagttcac accttcggga gtctgcatac aggggaccgg catcaagcct gatttagagg
     3781 taggcgtatt cgacgaatac aggaaccttc ctgtttcgca tatcccgagg gacagggatg
     3841 tccagctaag gtcggcggtc gaggcattgc ttggtgagat tgagcagccc gggaaataga
     3901 gtaagaagta tgaataaaag gtttttttct ccacaaacta ttaagggtaa tgctactaac
     3961 gtacggagag gtgtgaagat ggagaaaaaa acggatccca atgagaagct gaagtttgag
     4021 atcgccagcg aactggggct cagggagaaa gtcatgaagt atggatggaa gagcctgtcc
     4081 gccaaagaaa cgggcaggat cggcgggctg gtatcaaaaa ggagaaaggc gctgcagaat
     4141 ggagaccagg agacgtaaca gaaacgtgcg cagaggtatc acaggataga aacaggccac
     4201 ggatgcacag gaggcgtatg ggagccgcat atcaccgcat gcggcttttt tgccgcgaat
     4261 ttttgtggta tcattacgga tgggcatcat ggaagcaaag cggggtggcg gcgatttacg
     4321 gggattttgc atattattac gacaggctca tgtgggacgt ggactattcc atgtggaccg
     4381 attatgtgac ggatattttc aaatggcacg gctgtgagcc gaaaatgata gcggacctcg
     4441 ggtgcggtac cggaagcttc tgcctggaga tgtccaggag aggctttgac atgatcggaa
     4501 tcgacatatc cgccgaaatg ctgagcattg cgaagcaaaa ggctgtcagc gccaatgcag
     4561 atatcctctt cctgaaccag gacatgacgt ccttcgaact ctatggcacc gtggatgccg
     4621 ttacttgcct tatggacagc ataaactacg ttacatataa aaacgacctg aagcggcttt
     4681 tcaggcttgt gaacaattac ctgaaccccg gggggatctt tgttttcgat gtcaattcac
     4741 cgtacaaatt cgaaaggata ctgtcttcca acgtgttctg cgagtcatcg gaggatgtct
     4801 catacatctg gcagaacaca tatgataaac gttcggggct atgcaggttc gaccttacct
     4861 ttttcgtgaa ggaaggcaag tactacagga ggttcgatga gactcattat gagagaagct
     4921 acgggaccga tgaactgcag cagatgctcc tggctgcggg actgaccgtt cacgggatat
     4981 acggggcatt cggatccggg aagcctggtc cgaaaacaga aaggatattt ttcgcatgcg
     5041 ggaaacataa ataatattct tgtcggatat tgtataaaaa gcctgtgttt aggcaaacat
     5101 aaagttgcag gttgtttatg gtaaacgtcg aataacgccg attatgcggt ttatttgatt
     5161 gacaattcgt gagccggtgt gttacactga ttaaggtttg gcaaaagaag cgaaagcggt
     5221 cagacccaag tagaattcag gaggtttatc gtaatgcaaa aaggtagggt taagtggttt
     5281 aatgcagaga agggttttgg tttcatcgag agagaagacg gcaatgacgt gttcgttcac
     5341 tacaccgcga tccagatgga cggattcaaa accctcgaag aaggcgctga agtagagttt
     5401 gaagttgttg agggagcaaa aggccctcag gctgcaaacg tcacgaaggt tatgtaatta
     5461 aagttgtatc aactttaaca taaatccagt cagcacctaa gccccgatat caaatatcgg
     5521 ggtttttagt tggaaggacc gggtaaaatt gcttgtgtaa agattttaat ggcagaggag
     5581 tcgggatatg gaagaccaca tagtcagagc aacggcagcg gacggttatg tgcgcgcatt
     5641 tgccgctgtg accacgaacc tggtgcagga cgcccataag atccatgaag cttcgggagt
     5701 cgccactgtg gcgcttggca ggacacttac ggcggctgca atgatggcaa ggatgctgaa
     5761 aaacgacaag gatacgatga ctattcagat caggggggac ggtcctctgg gactgatagt
     5821 ggtcgtaacc gatgcgtttt ccaatgtccg gggatatgtc cacaacccgc aggtgtatct
     5881 gccgcttaat cccgcaggca agttcgatgt gtccgcagcc gtaggtaaaa acgggtatct
     5941 gaacgttata aaggatcttg gcctcaggga gccgtatata ggctatgtga aactggcatc
     6001 gggcgagatc ggagaagaca ttgcatacta tttcgctgcc tccgagcagg taccgacggc
     6061 cgtatcggtg ggagtcctcg tgggcccgga ccagaacgtg ctggcggctg gaggctatat
     6121 aatccagctt atgcccgatg caggcgaaga aataatcgat tatctcgaag aaaaagtgat
     6181 gtttgcggac tccataacct gcatgctgtc ggacgggatg gacgccgaag ccgtactgcg
     6241 gagcctgctg ggcgagaaag acctccagat aattgacagg ctgccatgca ggtaccagtg
     6301 cacctgcagc cgcgaccgca tggaaaggaa cctggtaagc ctgggcgcgg tagaactggg
     6361 caagatggcc gacgagcagc acggcgctga actgcagtgc cacttctgca ataagaagta
     6421 ttattttacc gaggaagaac tgagggcgct tataaaataa gggcccgcct gtcagataaa
     6481 ggtcttgcac gaactgctgc gcatccgcgc agcagttttt cgttatagct ttctttacct
     6541 tccgttaaaa agggttttct tcacttccgc agttttaccg ggcagtaagg ccgtgttatc
     6601 cagatactgc actatttggc aagatataaa tttaaatatt tacttttgta ttataatttg
     6661 ttaaaattaa ccatggaca
//
LOCUS       DAOSCC010000139         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7382_length_6665_cov_4.482753, whole genome shotgun sequence.
ACCESSION   DAOSCC010000139 DAOSCC010000000
VERSION     DAOSCC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7382_length_6665_cov_4.482753"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..343)
                     /locus_tag="PLG72_07995"
     CDS             complement(170..343)
                     /locus_tag="PLG72_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80776.1"
                     /translation="MNAERFHLKSLLLGIGIGIIITSIISLIYLSGRDPFEGISDEQI
                     IARAEKLGMIMKD"
     gene            complement(349..1026)
                     /locus_tag="PLG72_08000"
     CDS             complement(349..1026)
                     /locus_tag="PLG72_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80777.1"
                     /translation="MSGFYVSMIFIGVVLVLVSLVLIMIDRKNVFVFKNSADEKKKEL
                     IEIISDAEQMIEELNRFSGYIVDQMDLKNDELNRNIMEAEQKINALIEKVRLASEGQM
                     IPEKNAEVRKKDAVGCTEAKSLQLAAETVKAAVSASREMPDIYEAEKADGVRACIAAS
                     PAGSVAAAYSRNSARNAARKKDNVIQFNKYQEVLRLSREGMDELEIAKNLNMGKGEVE
                     LIIGLRR"
     gene            complement(1217..1546)
                     /locus_tag="PLG72_08005"
     CDS             complement(1217..1546)
                     /locus_tag="PLG72_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80778.1"
                     /translation="MSVKPVDFQIAIPRSIDAAKAVSDEIQKNAAMQQQQAASVQNRA
                     EDTLKQVYSRSKTEEVRITEKQREHERQGESRKKKDEDKEKNRGKKNSGKIYGGTITS
                     GRIDIKI"
     gene            complement(1588..3210)
                     /locus_tag="PLG72_08010"
     CDS             complement(1588..3210)
                     /locus_tag="PLG72_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOJ80779.1"
                     /translation="MLRNDTDYNVNDGFCELLFKEDGVYMCVYPPEGNGKRLELNAVI
                     DHLSRKKVRNFDRNKVEHAVIRADKVPVKIAEPQVEEKIDATVSVTVSPDKMKAYVTF
                     TPPDNGRMLTMEEVLDELSKNGVIYGINRTNLETLIKYPVYNEMICIAEGTPPVNGEN
                     GRVEFHFDIKRDHRPTILDDGRVDFRELNLIQNIEKGGVLCTLILPSPGIPGKTVTGT
                     DIPAADGKPAVLPKGRNVAVSEDGTKLIANISGQISYIDGKVSVFATHEVKADVDNST
                     GNINFIGNVVVMGNVLSGFTIEAGGSVEVMGVVEGAVIRAGGDIILRRGMQGMGKGIL
                     VSGGDIIARYIENSNIEARNDIKAEAIMHSNVKCGNKLELSGKKGLLVGGTCKVGKEI
                     IAKVIGSHLATVTDIEVGVDPTVRERYKAVREEIVQMETDLRKAEQAIAILKKMEMTG
                     TLTPDKKEMLAKSMRTKVYYSSRLLELKEEMSQLEAKLQQDAYGKIRCYDVIYPGTKV
                     AIGSCMMYVKENLQYCTLYRDGADIRVGPIDK"
     gene            complement(3264..4034)
                     /locus_tag="PLG72_08015"
     CDS             complement(3264..4034)
                     /locus_tag="PLG72_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ80780.1"
                     /translation="MQNDSKNIKEIWKKYQETRDPALRETLILTYSHLIKYIAGRLNI
                     YFGSNVEYEDLISYGVFGLIDAIDKYDMNKGVKFETYASLRIRGAIIDSIREMDWVPR
                     SLRQKCKELEKAYWEVENELGRSATDKDIADKLNISVEELHKLLNEVNVSSLISLEDF
                     LEQNYEIGVEPTSGRRQDRPEYEAEINELSEILGNAIDKLPEKEKMVLTLYYYEELTL
                     KEISAIMKVSESRISQLHTKAIMRLRGKLARQRSLLEE"
     gene            complement(4057..4461)
                     /locus_tag="PLG72_08020"
     CDS             complement(4057..4461)
                     /locus_tag="PLG72_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80781.1"
                     /translation="MGNIRKLPFLSGCSAAILAGIVSYAEGVENQTIYIRMAVIMFLF
                     YMIGVLVRNTVENAKKEIEEKKKEMEQEEARKRKKQKEEKMKEVLESRKNDVKHDDGT
                     EPGTTEEKREHDPNLDEEFRVLSDVIMTKAKE"
     gene            complement(4464..4952)
                     /locus_tag="PLG72_08025"
     CDS             complement(4464..4952)
                     /locus_tag="PLG72_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOJ80782.1"
                     /translation="MVKIIKVGMADLQSSVHPCIITTLGLGSCVGVTLYDPVRKVAGL
                     AHIMLPSSQQARNNSNIAKFADTAIVKLVNDMVALGADKSRLVAKIAGGAQMFSFADG
                     TSEMLRIGARNVSAAKEVLSSLRIPIVSEDTGGSYGRTIEIHSEDGRLKIKTIGHGTK
                     EI"
     gene            complement(4953..5588)
                     /locus_tag="PLG72_08030"
     CDS             complement(4953..5588)
                     /locus_tag="PLG72_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOJ80783.1"
                     /translation="MVRNINDLNSVHIDVLKEIGNIGAGNAATALAKLLNRKIDMDVP
                     RVKILDFKDVSETLGGAELPVVGIMLKVTGDLTGNILFILKQQAAAILVNMLMGKVSD
                     GADDAKVPEFSEIEISALKEIGNILAGSYLSSLSSLTNLNIVPSVPDLAFDMAGAILS
                     VPAIEFGKVGDTVLYIETEFTEGDKRVVGDFFLIPDVDTYSVLMNALGVSF"
     gene            complement(5625..6083)
                     /locus_tag="PLG72_08035"
     CDS             complement(5625..6083)
                     /locus_tag="PLG72_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ80784.1"
                     /translation="MADQNANEAKKYLVFRLEDDEFGIEISKISTIIEKDMDITRVPR
                     QPAFLKGVINLRGEIIPVLSLRLKFGLPDDVYNEDTRIVIIKLDDLSVGLIVDSVAEV
                     VELDDETTESVSNIAGETSLEYLKGVGKLNGRIITLLDLEKIIMLEEAAT"
     gene            complement(6128..>6665)
                     /locus_tag="PLG72_08040"
     CDS             complement(6128..>6665)
                     /locus_tag="PLG72_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ80785.1"
                     /translation="ITDLSGRGVGLDVVKTKIEALGGTVEVETELGMGSRFYIRLPLT
                     LAIIQALLVMVGEEKYAIQLSSVHKIINITPDDIKTVQKQEVVMYRNSVIPIVRLREV
                     LEVPGESNADQKVMTLVIVKKGERLTGFIVDRIIGQQEIVQKSLGKYLAGTKVITSAT
                     ILGDGNVALILDVNALAN"
BASE COUNT         1430 a         1864 c         1277 g         2094 t
ORIGIN      
        1 ggcaggatac tgcccgggaa aggacggagg atattatgtc atgggcaagc ctgcgcagga
       61 aaccatcagg cattacttct ccaaatggtg atctgccgca aagacatcca catggtactt
      121 acctatgctg caatcatatg ataaaaatgc cggctaatga tcgccggctc taatctttca
      181 tgatcatacc cagtttctcg gcacgggcta ttatctgttc gtcgctgatc ccttcgaacg
      241 gatcccttcc tgacagatat atgaggctta ttattgaagt tatgattatc ccgatgccga
      301 tcccaagcag caaactcttc agatggaaac gttccgcgtt cattccgctc atctccgcag
      361 gccgatgatc agttctacct cacctttgcc catattgagg ttcttcgcta tctccagttc
      421 atccatccct tcccttgaaa ggcgcagcac ctcctggtac ttgttgaact ggatgacatt
      481 atcctttttt cttgcggcat tacgcgcact gttcctgctg taggctgcgg ccacacttcc
      541 tgccgggctg gcggcaatac acgccctgac gccgtcagct ttttccgcct catatatgtc
      601 tggcatctca cggctcgctg aaaccgcagc tttaaccgtt tcagccgcaa gttggagact
      661 tttggcttca gtacagccga ccgcatcctt tttccgtact tcagcatttt tctccgggat
      721 catctgccct tcggatgcga ggcgcacctt ttcgatcagg gcattgatct tctgttctgc
      781 ttccattatg ttcctgttca gttcatcgtt cttgagatcc atctggtcta ctatgtagcc
      841 tgaaaatcgg ttcagttcct cgatcatctg ctctgcatca ctgatgatct cgatcagttc
      901 tttctttttt tcatcggcag aatttttgaa cacgaagaca tttttcctgt cgatcatgat
      961 gagcacaaga gataccagta ccaaaacgac accgataaag atcatactta cataaaatcc
     1021 gctcaattca ttcacctctc acagacgatc aggcatctct ccggacttgc gccatttccg
     1081 gcattggagt gcccgaaggt cttttttctt ttgtattgtg tatattattt tgattaacgt
     1141 ataccgtttt attataacat aacatataat atcctggtgt ccgttatctg atataatgcg
     1201 caaatatgcc gcaccctcag atcttgatat ctatcctgcc cgatgttatc gtacccccgt
     1261 atatctttcc gctgtttttc tttcccctgt tcttctcctt atcctcatct tttttcttcc
     1321 ggctttcccc ttgccgttcg tgctcccttt gtttttcagt tatccttacc tcttccgttt
     1381 tggagcgcga atatacctgt ttcagcgtat cttccgccct gttctgtacc gatgcagcct
     1441 gttgctgctg cattgcagca ttcttctgga tctcatcgct cacagctttt gccgcatcta
     1501 tcgacctcgg tattgcaatt tgaaaatcga caggtttgac agacatactc ctcacatcct
     1561 tgcccggaac agcgaagccg ggtcacatca cttgtctatc ggtcctaccc tgatatctgc
     1621 gccatccctg taaagcgtac agtattgcag gttctccttt acgtacatca tgcatgaccc
     1681 aatagctacc tttgtaccgg gatatattac atcataacac ctgatcttgc catacgcatc
     1741 ctgctggagc tttgcctcaa gctggctcat ttcttccttc agttcgagga gcctgcttga
     1801 ataatagacc tttgtcctca tactcttggc aagcatttcc tttttatcag gtgtaagagt
     1861 ccctgtcatc tccatttttt taagtatcgc aatcgcctgc tcagccttcc tgagatccgt
     1921 ttccatctgt actatctctt ccctgaccgc tttgtacctt tccctgaccg tcggatccac
     1981 gccgacttct atgtcggtga cagtcgcaag gtgtgaacct atgacctttg caatgatctc
     2041 tttgccgacc ttgcaggtcc ctccgaccag cagtcctttc tttcccgaaa gttcaagctt
     2101 gttgccgcac ttaacattgc tgtgcattat tgcttcagcc ttgatgtcgt tccgcgcttc
     2161 tatgttgctg ttctcaatat acctggctat gatgtcgcct ccgctgacca ggatgccctt
     2221 acccataccc tgcatccctc ttcttagaat aatgtcgccg ccagccctaa tgacagctcc
     2281 ttcgacaacg cccataactt caacgcttcc gcctgcttct attgtaaatc cggataatac
     2341 attgcccatc actaccacat ttcctatgaa gtttatgttg ccggtggaat tgtcgacgtc
     2401 cgcctttact tcgtgagtag caaaaacact gaccttgccg tctatatagc ttatctgccc
     2461 gcttatgttc gcgatcagtt tcgttccgtc ctcggaaacg gccacgttcc taccctttgg
     2521 caggacggcc ggcttgccgt ctgctgcagg tatgtccgtt cctgtcaccg ttttgccggg
     2581 gatcccggga gaaggcagta tcagggtaca cagtaccccg cccttttcta tgttctgtat
     2641 caggttcagt tccctgaaat caactctgcc gtcatcaagt atggtcggcc tgtgatcgcg
     2701 ttttatgtcg aaatggaatt caactctgcc gttctcgcca tttaccggag gcgtaccttc
     2761 agcgatgcat atcatttcat tgtataccgg gtatttgatc agggtctcaa gattggtcct
     2821 gtttatgccg taaatcacgc cgtttttcga aagctcgtca agaacctcct ccatagtcag
     2881 catcctgccg ttgtcgggag gtgtgaaagt gacatatgct ttcattttat caggcgatac
     2941 cgttaccgac acagtcgcat cgattttctc ttccacctgc ggttctgcga tttttaccgg
     3001 gaccttgtcc gctcttatga cagcgtgttc aaccttgttt ctgtcaaaat tccttacctt
     3061 tttcctggaa agatggtcga tgacagcatt cagttcaagc ctttttccgt tgccctcggg
     3121 gggatagacg cacatgtaga cgccgtcttc tttgaacagc agttcgcaaa aaccgtcatt
     3181 tacattgtaa tccgtatcgt tccgcaacat taagacaaga cctccgtaac ttccggtatt
     3241 acagcgtaat ttcccataaa taactattcc tcaagtaaag atctctgtct tgcaagtttg
     3301 cccctgagac gcatgattgc cttggtatga agctgtgata tgcgagattc cgacaccttc
     3361 attatcgcac ttatttcctt taaagtcaat tcttcatagt agtacagcgt caataccatt
     3421 ttttctttct ccggcagttt gtctattgcg tttccgagta tctcgctcag ttcgttgatc
     3481 tcagcctcgt attcaggcct gtcctgcctt cttccgctgg tcggctccac tcctatttca
     3541 tagttctgct ccaggaaatc ctccagtgat atcagtgagg atacattgac ttcattgagc
     3601 agtttgtgca gttcctcgac agatatgttc agtttatcgg ctatatcctt gtcagtcgcg
     3661 gacctgccca gttcattctc cacttcccag tatgccttct caagttcctt gcatttctgt
     3721 ctcagtgatc ttgggaccca gtccatttcc cttatactgt cgatgatagc tcccctgatc
     3781 ctcaatgaag cataggtctc aaattttacg cctttattca tatcgtactt gtcgatggca
     3841 tcaatcagtc caaatactcc ataactgatc agatcttcat attccacgtt cgatccgaaa
     3901 taaatgttca gcctccctgc tatatacttg ataagatgcg agtacgtaag tatcagggtc
     3961 tctctcaaag caggatctct cgtttcctga tactttttcc atatttcctt tatgttttta
     4021 gagtcattct gcatttaaat cccccgtata cccggtttat tcttttgctt ttgtcatgat
     4081 aacatcgctg agcactctga actcctcatc caggttcgga tcgtgttccc ttttttcttc
     4141 cgtcgttccc ggctctgttc catcatcatg ctttacatcg tttttccgcg attcaagcac
     4201 ctctttcatt ttttcttctt tctgtttctt tcttttcctg gcttcttcct gttccatttc
     4261 cttctttttt tcttcgatct ccttcttagc attttcaaca gtgtttctta ccaggactcc
     4321 gatcatataa aaaagaaaca ttataaccgc catccggata tatatcgtct ggttttcgac
     4381 tccttcggca taacttacga tacccgccag gatcgctgcc gaacacccgg ataagaacgg
     4441 taatttccga atgtttccca tacttatatc tcctttgtcc cgtgtccgat agtttttatc
     4501 ttaagcctgc cgtcttctga atgtatctca atggtccttc cgtagctgcc gcctgtgtcc
     4561 tcggaaacta tggggatccg cagcgaactc agcacttctt tcgccgctga cacattcctt
     4621 gctcctattc tgagcatttc agacgttccg tcggcaaatg agaacatctg cgctcctccg
     4681 gctattttgg ccacaagcct tgatttgtcg gcgcctaaag ccaccatgtc attgaccagc
     4741 ttcacgatcg cagtatctgc aaactttgct atattcgaat tgttgcgagc ctgttggctc
     4801 gaaggaagca ttatatgagc cagtcctgca acctttctga ccggatcgta caaagtcact
     4861 ccaacacatg atccaaggcc aagcgtcgtg ataatacagg gatgcacgga cgactgcaaa
     4921 tcagccattc ccacctttat gatctttacc attcagaaac taactcccaa tgcattcatt
     4981 aacacgctgt aagtatctac atcaggtatc aggaaaaaat ccccgacgac ccttttatca
     5041 ccttcggtaa attcagtctc gatataaagc acggtatcgc cgactttgcc gaactctatc
     5101 gcaggcacgc tgagtatggc tccggccatg tcgaaagcca gatcgggcac tgacggcacg
     5161 atattgaggt tcgtcagcga agacagcgat gacagatatg acccggcaag tatgtttccg
     5221 atctccttga gcgccgagat ctcgatttcg ctgaattcag gaactttcgc atcatcagcg
     5281 ccgtccgaca cctttcccat gagcatgttg acgagtattg ccgcagcctg ctgcttgagt
     5341 atgaacaata tattcccggt aagatcgccg gtgaccttga gcattatacc gaccaccggc
     5401 agttctgctc ctccaagggt ctcactgaca tccttgaagt ctaatatctt taccctggga
     5461 acatccatat ctatcttcct gtttaaaagc tttgccagcg ctgttgcggc gttccccgcg
     5521 cctatgttgc ctatctcctt caaaacatcg atatggacac tgttaagatc atttatattc
     5581 cttaccatgg ctcattctcc cgttcaaacc tgcacatcct gtacctatgt ggcagcctcc
     5641 tccagcatta tgatcttctc cagatccaga agcgttatta tcctgccgtt gagttttccc
     5701 acacccttaa ggtattcaag cgatgtctcg cctgctatgt tggaaacact ctcagtagtt
     5761 tcatcgtcca actccacgac ctctgcaaca gagtccacga tcagcccgac ggacaggtca
     5821 tccagtttaa ttatgacgat tctggtatct tcattgtata catcatcagg aaggccgaat
     5881 ttaaggcgca ggctcagtac aggtatgatc tcgcccctta agttgatcac tcccttaagg
     5941 aaagccggtt gtctcgggac cctggtaata tccatgtcct tctcgattat tgtcgatatc
     6001 ttgcttattt ctataccgaa ctcatcgtcc tcaagcctga aaaccagata ctttttagct
     6061 tcgttcgcat tttgatccgc catatctgaa ccccctccga gtatatccta tttgcagctg
     6121 ttaagattca gtttgccaaa gcgttgacat cgagtatgag tgcaacattt ccgtcaccaa
     6181 gtatggtagc gctcgtgatc acttttgtcc ctgccaggta tttacccagg gatttctgaa
     6241 ctatctcctg ctgccctatt atcctgtcga caataaagcc tgtcagccgc tcgcccttct
     6301 tgacgatgac aagggtcatc actttctggt cggcattgct ttctccgggc acttcaagca
     6361 cttccctcag cctgacgatc gggatcacgg aattcctgta catgacgact tcctgctttt
     6421 gcactgtttt tatatcatcc ggcgtgatat tgattatctt atgtaccgaa ctaagctgga
     6481 ttgcatactt ttcctcgccg accatgacga gcagagcctg gattattgca agtgtgagcg
     6541 gcagcctgat atagaacctg cttcccatgc caagttccgt ttctacctcg accgtaccgc
     6601 caagagcctc gatctttgtt tttacaacgt ccaggccaac acctcttccg gaaaggtcag
     6661 taatt
//
LOCUS       DAOSCC010000140         6495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7596_length_6495_cov_4.328416, whole genome shotgun sequence.
ACCESSION   DAOSCC010000140 DAOSCC010000000
VERSION     DAOSCC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7596_length_6495_cov_4.328416"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..518)
                     /locus_tag="PLG72_08045"
                     /pseudo
     CDS             complement(1..518)
                     /locus_tag="PLG72_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161180.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
     gene            complement(606..1613)
                     /gene="asnA"
                     /locus_tag="PLG72_08050"
     CDS             complement(606..1613)
                     /gene="asnA"
                     /locus_tag="PLG72_08050"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOJ80786.1"
                     /translation="MSQLTVPKGYKSLLSLYETQIAIGMIKRIFEDNLSKALNLKRVS
                     APLFVPANTGINDDLNGVERPVEFDIKETKTCAQVVQSLAKWKRLALYKYGFSSGEGI
                     YTDMNAIRRDEEMDNLHSIYVDQWDWEKVISREERNLETLKDTVRKIVGAICDTLDEI
                     KKTYPVLTTYLSREVFFITTQELEDMYPELSPKERENAYVKEHRTVFIMQIGDVLRSG
                     KKHDGRAPDYDDWKMNGDILFWNDVLQCALEISSMGIRVDSKSLDEQLTKARCDDRRE
                     FMFHKMLLNDELPLTMGGGIGQSRLCMLLLQKAHVGEVQVSVWDRDTIEGCAAAGINL
                     L"
     gene            2063..3154
                     /locus_tag="PLG72_08055"
     CDS             2063..3154
                     /locus_tag="PLG72_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80787.1"
                     /translation="MMKEGMFRNVSGCAISGIFSKSGRRFSGKLIIDSITVMHERSNG
                     LGGGFAGYGIYPEYKDYYAFHIFYENKSAKKECEEYLEEHFDIINLSKIPTRKVPQIT
                     DEPLIWRYFVAPQPTKLADSQLDEREYVVRSVIRINTAIDGAYVFSSGKNMGVFKAMG
                     YPEDVGRFYRLEEYEGYCFTAHGRYPTNTPGWWGGAHPFALLDYSVVHNGEISSYDAN
                     RRCIEMYGYKCTLQTDTEVITYIIDYLHRKKGLTLKEICSVIAAPFWSTIERKPQKER
                     EELEYLRSAFDTLLITGPFSIIVGFEGGMMALNDRLKLRSMVVGEKDDMVYVASEEAA
                     IRVIQSDLDKVWAPRGGEGVIITLENGES"
     gene            3154..4659
                     /locus_tag="PLG72_08060"
     CDS             3154..4659
                     /locus_tag="PLG72_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOJ80788.1"
                     /translation="MAVQYIYPEYEVVRNYDRCIACRVCERQCANEVHEYLADSNKMV
                     ADDSKCVNCHRCVALCPTRALKIVKTDHTFKENANWSGKSITEIYKQAENGGVLLSSM
                     GNPENYPVYFDKLLLNASQVTNPSIDPLREPMETRVFLGAKPEKIERDERGRLKNNLP
                     PQIILSMPVMFSAMSYGSISYNAHESLARAAAELGILYNTGEGGLHENLYKYGKHTIV
                     QVASGRFGVHKTYLDSGAAIEIKMGQGAKPGIGGHLPGAKIVGDISKTRMIPEGTDAI
                     SPAPHHDIYSIEDLRQLVFSLKEATGYTKPVIVKIAAVHNVAAIASGIARSGADIIAI
                     DGFRGGTGAAPTRIRDHVGIPIELALASVDKRLREEGIRNNVSIVVGGSIRSSADVVK
                     AVALGADCVYIGTAALIALGCHLCRSCHTGKCNWGIATQREDLVKRLNPDLGYRRLVN
                     LMTAWKHEIKEMMGGMGINSIEALRGNRLMLRGVGLNEKELEILGIKHAGE"
     gene            4738..5172
                     /locus_tag="PLG72_08065"
     CDS             4738..5172
                     /locus_tag="PLG72_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ80789.1"
                     /translation="MKRVYVNEQWCLGCHLCEYYCAFASTNEKDMARALKDVKINPRI
                     RIEEKDGISFAVSCRHCDEPLCVKSCITGALTKVNGIIMIDKNRCIGCYTCVLSCPYG
                     AVYPSEDGAIQKCELCIKTHEGTPRCVTGCPNGAIVFEERGE"
     gene            5174..6421
                     /locus_tag="PLG72_08070"
     CDS             5174..6421
                     /locus_tag="PLG72_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80790.1"
                     /translation="MKYVIVGNSAAAVGAVEAIRRNDKEGSITIISDEKHHVYSRPLI
                     SYLLLGKTDENRMKYRDDGFYIDNGCELLSGKTAVKIDPGEKRVILDDGASVSYDRLL
                     LCTGSSPFVPPVPGLDKVKSKFTFTTLDDAKALNDALKENSRVLIVGAGLIGLKCAEG
                     ISKKKVKITCIDLAPRVLSSILDDESSEIVKRHLEEHDIRFYLNQQVMELEEDTAYLG
                     DNTAIRFDVLVMAVGVRPNISLLKDIGGKTNRGIIINDRCRTSIPDIYAAGDCCESID
                     ISSGEAKVMALLPNAYMQGECAGMNMSGADFVFDKAIPLNSIGLLGLHIMTAGTYKGE
                     VYAETGEKSCKKLFYSDNRLNGFILIGNIEKAGIYTSLIRERTPLDTVDFELICKMPG
                     LMAFSRKERAEKLGGVRDESIGA"
BASE COUNT         1706 a         1530 c         1753 g         1506 t
ORIGIN      
        1 cgcctgcctg ttgagggcct gcatggagcg caggagggcg tctttttatc cagggcttct
       61 ccgctgtacg aacagaaagc tgtgggaata tacagcgtac tgccttttac gaaagcatag
      121 gaagtcggat cccatgcggt gtatcctctt gcctcgaagg tggatctcag tcctcctgaa
      181 ggaaagctgg atgcgtcagg ttcgcctttc actagcgatt tgcctgaaaa ctccatgatg
      241 gccctgccac ggccgtccgg agtaatgaaa ctgtcatgct tttcggcggt cacgcctgtc
      301 atgggctgga accaatgggt gaaatgggtc gcgcccttct ctatggccca gtctttcatg
      361 acggctgcca ccacgtttgc tacgtcgagt tccaggtggg taccgttttc aatggttttc
      421 ctcagcgcat tatagatttc tttcggcagg cgttctttca ttaccgcgtc tccgaatacc
      481 atactgccga agagttcgga tatattctta tttgccattt ttgcaccctc tttccagaaa
      541 attacagcat tgattaaaat aataccaata gaaacagttc tccgacttag caggtatatt
      601 accgctcaaa gcaaatttat tcccgctgcg gcacagcctt cgatcgtatc ccggtcccag
      661 acggaaacct ggacttcacc gacatgggct ttctgcagca gaagcatgca aagtctcgac
      721 tgtcctatac cgccgcccat cgtcagcggc agttcatcat taagaagcat tttgtggaac
      781 atgaattcgc gccggtcgtc gcacctggcc ttggtaagct gttcatcaag agatttactg
      841 tcgacgcgta tacccatgga tgatatctcg agagcgcact gaaggacgtc gttccagaag
      901 aggatgtcgc cgttcatttt ccagtcgtca tagtccggcg ctcttccgtc atgctttttg
      961 cctgacctca gcacatctcc tatctgcatt atgaagaccg tcctgtgctc tttcacatat
     1021 gcgttttcac gttccttggg cgaaagctca gggtacatgt cttccagttc ctgggtggtg
     1081 atgaagaaaa cttcccttga aaggtatgtt gtaagtaccg ggtatgtctt tttgatctca
     1141 tccagcgtgt cgcatattgc gccgacgatt ttcctgaccg tatccttcag ggtttccagg
     1201 tttctttctt cacggcttat aaccttctcc cagtcccact ggtcgacata tatcgaatgg
     1261 aggttatcca tctcttcgtc acggcgaatt gcgttcatat cggtgtatat cccttcgccc
     1321 gaggagaacc cgtacttgta cagcgccagg cgtttccact ttgccagcga ctggacgacc
     1381 tgggcgcagg ttttggtttc cttgatgtcg aattccacag ggcgttcaac accgttcaga
     1441 tcgtcattta tccccgtatt tgcaggaacg aacaacggtg ccgagactct tttcagattg
     1501 agagccttgc tgaggttgtc ctcaaatatc ctctttatca tcccgatcgc gatctgggtc
     1561 tcataaaggc taagaagcga tttgtatcct tttggcactg tcagctgact catgaccaca
     1621 ctcctctcag tgaaaatacc tgtaaataat aaataaagat gccgcggacc ggaagctcca
     1681 cagcaccttt gctgttacag gcaattccac agcacctttg ctgctagcaa aagattaaca
     1741 cggcagtaag ccgatgtcaa tacttttctt ccatcttaaa aataaaaatg caatttgcat
     1801 aattcgaact tgcaaaaatc aaaaaccggt caggcatatt caccaaaacc aaattaaaat
     1861 atgcaatttg taatataatg cgattcataa aacagttgac acatataatg ttaatgtagt
     1921 atcattatca aaacgaacaa aggcgttcat cagatagtgg aggcgatagc cgaagaggct
     1981 gcgccgcctg gaaaggcact atgtgatgga cgccttaatt attttggttc atggtatttt
     2041 ggttcaaagc cggggggtga agataatgaa ggaaggaatg ttcagaaacg tatcaggatg
     2101 cgccatatca gggatctttt caaaatcggg cagacgtttt tccggcaagc tcataatcga
     2161 ttccatcacc gtcatgcatg aacgctccaa cggtcttggc ggaggtttcg cgggatacgg
     2221 gatctaccct gaatataagg attattatgc cttccatata ttttatgaaa acaaatctgc
     2281 gaagaaggaa tgcgaggagt acctggaaga gcatttcgac attataaacc tttcgaagat
     2341 accgacgcgg aaggtgccgc agatcactga cgagccgttg atctggaggt atttcgtcgc
     2401 accccagccg acgaagcttg ccgacagcca gcttgacgag agggaatacg tagtaagaag
     2461 cgtcatcagg ataaatacgg ctatcgatgg tgcgtatgta ttctcaagcg gaaaaaacat
     2521 gggagtgttc aaggcaatgg gatatcccga agatgtagga aggttctacc gccttgaaga
     2581 gtacgagggt tactgtttca cggcgcacgg caggtatccc accaatacgc cgggctggtg
     2641 gggaggggcg catccctttg cactccttga ttactcggtg gtccacaacg gcgaaatatc
     2701 ttcatacgac gccaaccgcc gctgtatcga gatgtatggc tacaaatgca cgctgcagac
     2761 agataccgaa gttattacct atatcataga ttatctgcac aggaagaaag gacttaccct
     2821 gaaggagatc tgctcggtga tcgcggcgcc gttctggtcc acgatagagc gtaaaccgca
     2881 gaaggaaagg gaagaactcg agtacctgag gagcgctttc gatacactgc tgatcactgg
     2941 cccgttttcc ataatcgtgg gattcgaagg cggcatgatg gccctgaacg acaggctgaa
     3001 gctccgcagc atggtagtag gagagaaaga cgatatggtc tacgttgcca gtgaggaagc
     3061 cgccataaga gtcatacaga gcgacctcga caaggtatgg gctcccaggg gcggcgaagg
     3121 agtcattatc acactagaga atggagaatc gtaatggctg ttcagtacat ctatccggaa
     3181 tatgaagtgg taaggaatta tgaccgctgc atcgcctgca gggtctgtga acggcaatgc
     3241 gcgaacgaag tgcatgaata cctggcggac agcaacaaga tggttgccga tgattccaaa
     3301 tgcgtgaact gtcaccggtg tgtggctctc tgcccgacac gggctctgaa gatcgtcaaa
     3361 acggatcata ccttcaagga gaacgccaac tggagcggca agagcatcac ggagatctat
     3421 aaacaggcgg aaaacggagg cgttttgctg tcaagcatgg ggaaccctga aaattatcct
     3481 gtgtattttg ataaattact gctcaacgct tcccaggtca cgaatccttc catcgaccca
     3541 ttgagggagc cgatggagac cagggttttt cttggcgcaa agcccgaaaa gatcgagagg
     3601 gatgaaaggg gccgcctgaa gaacaatctg ccgccgcaga taattctttc catgccggtc
     3661 atgttttcgg ccatgagcta cggctcgata agctacaacg cgcatgaaag ccttgcaagg
     3721 gccgcagctg agctagggat cctatacaac accggtgagg gcggtctgca tgagaacctt
     3781 tacaaatatg gaaagcatac gatcgtgcag gtggcttcag gacgattcgg agtccacaag
     3841 acttacctgg acagcggggc tgccatcgag atcaagatgg gacagggtgc aaagcccggc
     3901 atcggcggac atctgcctgg tgcaaaaata gtgggggata tttcgaagac ccgtatgata
     3961 cctgaaggca ccgatgcgat atcgccggct ccccatcatg acatttactc cattgaagac
     4021 ctgcgccagc tggtattctc actgaaggaa gcgaccggct acacgaagcc tgttatcgtc
     4081 aagatcgctg ccgtgcacaa tgttgcggcg atcgcaagcg gcattgcacg cagcggggcg
     4141 gacatcatcg ccatagacgg tttcaggggc ggaacgggcg cagctcccac gcggatccgc
     4201 gaccatgtgg gcatcccgat cgaactggca ctggccagtg tcgacaaaag gctgcgcgaa
     4261 gaggggatcc gtaataacgt atccatcgtt gtgggaggga gtatccgctc cagtgccgac
     4321 gtcgtgaaag cagtcgccct cggcgctgac tgcgtttata tcggaactgc ggctctcatt
     4381 gccctgggat gccatttgtg caggagctgc catacgggca aatgcaactg gggcatcgcc
     4441 acccagagag aggatcttgt taagcgcctc aacccggatc tggggtacag gaggctcgtg
     4501 aacctcatga ccgcctggaa gcatgagatc aaggaaatga tgggcggtat ggggatcaac
     4561 tcgatcgaag cgctgagagg caacaggctc atgctccggg gcgtcggcct gaacgagaag
     4621 gagcttgaga tactcggcat aaagcatgca ggggagtgat accggcatac tgacagccgg
     4681 ttcagaactg gttaatttga gcaacggtat tgaaattatt gtgacggtgg ttgagaaatg
     4741 aaacgtgtat atgtgaatga acaatggtgc ctggggtgtc atttgtgcga atattactgc
     4801 gcttttgcgt cgaccaacga gaaagacatg gcaagggcgc tgaaagacgt aaagatcaat
     4861 cccaggatca ggatcgaaga aaaagacggg ataagttttg cggtttcctg ccgccactgc
     4921 gatgaaccgc tctgtgtcaa aagctgtata acaggcgcgt tgacaaaagt aaacggcatc
     4981 ataatgatcg ataaaaacag gtgcataggg tgctatacct gcgtcctgtc ctgcccctac
     5041 ggcgcggtct atccttctga agacggagcc atacagaagt gcgagctttg cataaaaacg
     5101 cacgagggga ctcccaggtg cgtgaccgga tgtcccaacg gggcgatcgt gttcgaggag
     5161 agaggtgaat agcatgaaat atgtcatagt cggcaattct gctgccgcag tcggcgctgt
     5221 ggaggcaata cgcaggaatg acaaggaagg aagcattacc attataagcg atgaaaaaca
     5281 tcatgtttat tccaggcctc ttatctcata cctgttattg ggcaaaacgg atgaaaacag
     5341 gatgaaatac agggatgacg gcttttatat cgataacggc tgtgaacttc tgtcgggtaa
     5401 aaccgccgtg aagatagacc ccggggaaaa gcgggtgatc ctcgacgacg gggcatctgt
     5461 aagttatgac aggctgctgc tgtgtaccgg gtcttcgccg ttcgtccctc cggtgccggg
     5521 actggataag gtcaaaagca aattcacctt taccacgctc gatgacgcta aagcgctgaa
     5581 tgatgcactt aaggagaatt caagggtact gatcgtggga gcaggcctca taggtctcaa
     5641 atgcgccgag ggcatcagca aaaaaaaggt gaagatcact tgtatagacc tggctcccag
     5701 ggtgctgtca agcattttgg atgatgaaag ctcggagatc gtgaagaggc acctggaaga
     5761 acacgatatc aggttttacc tgaatcagca ggtcatggag ctcgaagagg acacagcata
     5821 tctgggcgat aataccgcca tacggttcga tgtgctggta atggcagtgg gagtgcggcc
     5881 gaatatatcg ctgctgaagg atattggagg caaaacgaac aggggtatca tcatcaatga
     5941 taggtgccgg acttccatac ccgatatata tgccgcgggc gactgctgtg aaagcataga
     6001 tatttcctcg ggcgaggcga aagtcatggc tctgctccca aatgcatata tgcagggaga
     6061 atgcgcgggg atgaacatgt cgggggcgga tttcgttttc gacaaagcca ttccgctgaa
     6121 ttccatcggt ttgctcggac tgcacataat gacagctggc acctataaag gcgaggttta
     6181 tgcagagaca ggcgagaaaa gctgcaagaa gctgttttac tcggacaaca ggctgaacgg
     6241 cttcatactg atagggaata tagaaaaagc gggcatttac acatcgctga taagggaaag
     6301 gaccccgctt gatacggtcg attttgaact aatatgcaaa atgcccggac tgatggcctt
     6361 cagcaggaaa gaacgggccg agaaactggg aggtgtcagg gatgaatcta ttggcgcgtg
     6421 aactgggatt caggaaactg aacgaaatga tccgcaatac aaatgaagat gtggtgatcg
     6481 actgctgcta cggcg
//
LOCUS       DAOSCC010000141         6392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7754_length_6392_cov_4.534480, whole genome shotgun sequence.
ACCESSION   DAOSCC010000141 DAOSCC010000000
VERSION     DAOSCC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7754_length_6392_cov_4.534480"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PLG72_08075"
     CDS             complement(<1..170)
                     /locus_tag="PLG72_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ80791.1"
                     /translation="MLQAWYDIVEFLLPFEWAEHNFMKNALLAVLLITPLFGLLGTMV
                     VSNRMAFFSDSIG"
     gene            complement(170..973)
                     /locus_tag="PLG72_08080"
     CDS             complement(170..973)
                     /locus_tag="PLG72_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOJ80792.1"
                     /translation="MHTDRYHAKCDGNCCNLCCTRIENLGVTKGHTTILENVNIHIHC
                     GELTAIIGPNGGGKSTLLKALLGEVPYTGEFRFLNARGDRSIEPVIGYVPQHLDFDAG
                     IPASVSDLFIACLSKKPAWLMRPGSIRKRALEGLRKVNAEHLLDRRLGALSGGELQRV
                     LLALALDPVPNLLLLDEPVSGMDQGGLELFYSTVAELRHNYDLSIILVSHDFDQVYRH
                     ADRVVLLNRTVITSGTPQQVFSDEKTLKCFGITGFFADGMHGGPEGGNG"
     gene            complement(992..1915)
                     /locus_tag="PLG72_08085"
     CDS             complement(992..1915)
                     /locus_tag="PLG72_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOJ80793.1"
                     /translation="MYRRLATIAAVLLAAAMLLTGCRTADDKDGGSNAAGDGQDGLHV
                     VTSFFPVYVAVINVTDGVPGVTVTNMTEPQTGCLHDYSLKPSDLRLLEKADVFIINGA
                     GMESFLDDVLSQQEDLEIIEAAAGIPAIVEENGEENPHVWVSPTNVTAYVQNIADGLA
                     ALDEKNAELYQKNAASYIRKIETLKQEMHNELDGLEYKDIITFHEAFPYFAQEFGLNI
                     AAVVEREPGTEPTPKELAEIITLVRETGVRALFAEPQYSSGAMDTIAGETGAVVYTLD
                     PVVTGEAVPGARDAYLDAMRRNMETLKEALG"
     gene            2154..2351
                     /locus_tag="PLG72_08090"
     CDS             2154..2351
                     /locus_tag="PLG72_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80794.1"
                     /translation="MSVNGMMVQYIGSIRQALAVAALRKNLGQDVRTMDALLQGFQNT
                     NAKIMENSVTPHKGGNIDIRV"
     gene            complement(2452..4437)
                     /gene="tkt"
                     /locus_tag="PLG72_08095"
     CDS             complement(2452..4437)
                     /gene="tkt"
                     /locus_tag="PLG72_08095"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ80795.1"
                     /translation="MNNVELLTVNTIRILSAEAVQKANSGHPGLPMGAAPMAYTLWAR
                     NLKHNPANPGWDDRDRFILSAGHGSMLIYSLLHLFGYGLTIDDLKNFRQLDSLTPGHP
                     EYGHTKGVEITTGPLGQGLANAVGMAMAEAYLANRFNRPGYPVVDHYTYVLAGDGCMM
                     EGVASEAASLAGTLGLGKLIVLYDSNSITIEGSTDIAFREDVRKRFEAYGWHTLKVED
                     GTSIEDIDAAIKAAKAVKDKPSLIEIKTQIGYGSPKQGMASAHGEPLGAENVLETKKT
                     LGWDYTEEFHVPDEVKAYMEQIRANGMKAENEWNKMMEAYKKEYPELAAEWEAWKEKD
                     YSGTLLDDEDFWSYDSKANATRNSSGEVLNKLAARIPNLIGGSADLAPSNKSYMKNRG
                     DFSREDRSGSNLHFGVREHAMAAIANGIAAHGRLVPYVATFFVFCDYMKPSMRLSALM
                     KLPVTYILTHDSIGVGEDGPTHQPVEHLAALRSIPNFIVFRPCDAKETAAGWYTAMSR
                     KNTPTALILTRQNLPQLEMTGKDALKGAYVILDSGKKPEIILMASGSEVHLVYEAGKQ
                     LLERGIAVRVVSMPSWELFEEQDEAYKESVFPPDVVKRLAVEAGASFGWHKYTGLQGE
                     IISIDHFGESAPAGLLFKRYGFTVENVLEKALKLLNM"
     gene            complement(4546..5304)
                     /locus_tag="PLG72_08100"
     CDS             complement(4546..5304)
                     /locus_tag="PLG72_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOJ80796.1"
                     /translation="MGTEAVYGHTYTIGYSDVDFMKYLKCSKLFEYFQDTASEAVDRL
                     GAGVGTLAEKYSAAWVLTRIRVEFTRIPGLNESITVATWPHPPGKIEFERDFRVCDRN
                     GDAIIRAKSIWVIIDTKTRELRKSDYIHMDYPEYTEGHALDSRPGKLRPSFKPEPVYR
                     KTVGYSDVDFNGHINNSRYIDYIMDCFSVESHKQYMIKAMEVNYIKEAFPGDTLLLCR
                     EIPRDGGNTVYIEGLNEEDGRPCFKARIWAEPRK"
     gene            complement(5739..>6392)
                     /locus_tag="PLG72_08105"
     CDS             complement(5739..>6392)
                     /locus_tag="PLG72_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOJ80797.1"
                     /translation="GHGTSLGGVIVDGGKFDWIGSGKFPGLTEPDPSYHGIVYARDVG
                     AAAYVTKIRVQLLRDTGAALSPFNAFLLLQGLETLSLRVARHVDNAMKIAKYLNGHPQ
                     VAWVNYPGLENNKYHELARKYMPKGAGSIFTFGIKGGLEAAVKFIDSLEILSLLANVA
                     DAKSLVIHPASTTHAQLSPEEQKAAGVTPDQIRLSIGIEDPEDLIYDLEQALRKAAE"
BASE COUNT         1337 a         1855 c         1564 g         1636 t
ORIGIN      
        1 cctatggaat cggaaaagaa tgccatcctg ttgcttacga ccatcgttcc aagcaggccg
       61 aacagcggtg ttatcaaaag caccgccagc agcgcgtttt tcatgaagtt gtgttccgcc
      121 cattcgaaag gcagcaggaa ttccacgata tcataccagg cctgcagcat catccgttac
      181 ctccctccgg acccccgtgc atcccgtccg caaaaaaccc ggtgatcccg aaacatttca
      241 gggtcttctc gtcactgaac acctgctgcg gagtgccgct ggttatcacg gtcctgttga
      301 gcaggaccac ccggtccgcg tggcggtaaa cctggtcaaa gtcatgggaa acaaggatga
      361 tggaaagatc gtaattgtgc ctgagttccg ccacggtgct gtaaaacagt tcaagcccgc
      421 cctggtccat ccctgaaacg ggttcatcga gaagcagcag gttgggcaca ggatcgagag
      481 ccagcgccag cagcaccctc tgcagttctc ctccggacag cgcccccagc ctgcggtcca
      541 gcagatgctc tgcgttgacc ttcctcaggc cctccagcgc cctcttcctg atactgcccg
      601 gcctcatgag ccacgccggt tttttcgaaa ggcacgcgat aaacaggtcg gacacacttg
      661 caggtatgcc agcatcaaaa tccaagtgct gcggcacata cccgatcacc ggttcaatgc
      721 tccggtcgcc cctggcattt aagaacctga attcgccggt atacggcact tcaccgagca
      781 atgccttcag cagggtgctt ttaccgccgc cgttggggcc tattatggcg gtcagttcac
      841 cgcagtgtat atggatattg acattttcga gtatggtggt atggcccttt gtcacaccaa
      901 ggttttcgat ccttgtacag caaaggttgc agcagtttcc gtcacatttc gcatggtacc
      961 ggtccgtatg catgatctgc ctcaccgcgt tttatcccaa agcttctttc aatgtctcca
     1021 tgttccggcg catcgcgtcc aggtatgcgt cccgcgcccc aggaacggcc tctcccgtga
     1081 ccacgggatc cagtgtatac acaacggcac ccgtctcgcc ggctatcgta tccatcgctc
     1141 ccgatgagta ctgcggctca gcaaaaagcg cccggacgcc cgtttctctg accagggtta
     1201 tgatctctgc cagttccttc ggagttggct cggtgcccgg ttccctttcg acgaccgcgg
     1261 cgatgttgag gccaaactcc tgtgcaaaat acgggaatgc ctcatggaac gttataatat
     1321 ccttgtattc caggccgtcc agttcattat gcatttcctg tttcagcgtc tcgatcttgc
     1381 gtatgtatga agcggcgttc ttttgataca gttccgcatt tttttcgtcc agggctgcca
     1441 gcccgtccgc tatattctgc acgtatgctg tcacattggt cgggctgacc cagacatgcg
     1501 ggttctcttc gccgttctcc tccacgatcg ccgggatacc ggccgctgct tcgattatct
     1561 ccaggtcttc ctgctggctc agcacatcgt cgagaaacga ctccatcccg gcgccgttta
     1621 taatgaacac gtcagccttt tcgagcagtc tgaggtctga aggtttgagc gaataatcgt
     1681 gcaggcatcc tgtctggggt tcagtcatgt tggtcaccgt gacgccgggc acaccgtccg
     1741 tcacattgat cactgccaca tatacaggga aaaaggacgt cacgacatgc agtccgtcct
     1801 gcccgtcccc ggcggcattg ctcccgccgt ctttatcatc tgcagtcctg cagcctgtaa
     1861 ggagcatcgc cgcagccagc agtaccgctg caattgttgc cagccttctg tacattatta
     1921 ccctccaggt tctttcatat gcctgtattc taccacatga cagccaaaca gcaaacaggg
     1981 atcgtttgtg tcaggcctga acgttccggt ccgatgcggc cggaacaggc ggtcctgggc
     2041 caaataggcc ttccgcccca tatacttctg atgaaaaatg atgttacaat gattatgagg
     2101 tatctgatat ccaatatcag catcccgcct gttgaatgcg gggtggtgtc cgaatgagcg
     2161 tgaacgggat gatggtccag tacataggca gtatacgcca ggctcttgct gttgccgcac
     2221 tcaggaaaaa cctcggccag gatgtccgga caatggacgc actgctgcag ggttttcaga
     2281 acacgaacgc aaaaatcatg gaaaactcag tcacgcccca taaaggcggg aacatcgata
     2341 tacgagtata atgctgcaat acttcatcat aacactgcca gggacggtcc agcctgtctc
     2401 ccgaatctga tcccgtggga acaccggcat acggaccgtc cctttttatc attacatgtt
     2461 cagcagtttc aatgcttttt ccaatacatt ctctacggtg aatccgtatc tcttgaacag
     2521 gagccctgcg ggagccgatt caccgaaatg gtctatcgag attatctcgc cctgcaggcc
     2581 ggtgtactta tgccagccga aggaagcgcc cgcttccacc gcaagccttt ttacaacatc
     2641 aggcgggaac actgattcct tataagcttc atcctgttct tcgaacagtt cccatgacgg
     2701 catgctgacg acgcggacgg caataccgcg ctccaacagc tgtttgcctg cttcgtagac
     2761 caggtgcact tcggatccgg aggccataag tatgatttca ggcttcttcc ctgaatccag
     2821 tatgacataa gctcccttga gcgcgtcctt gccggtcatt tcaagctgcg ggagattctg
     2881 tctcgtaagt atgagcgctg tgggcgtgtt tttcctggac attgccgtat accatcctgc
     2941 ggctgtctcc tttgcatcgc acggacggaa cactatgaag ttggggatgc tcctgagcgc
     3001 cgccaggtgc tccacaggct ggtgggtagg cccgtcctca ccaacgccga tactgtcatg
     3061 ggtcagtata tatgtcacag gaagcttcat caacgcggaa agcctcatgg acggcttcat
     3121 ataatcgcag aacacgaaga acgtagcaac atacggcaca agcctgccat gggcggcaat
     3181 accgttcgct atcgctgcca tggcgtgttc acgcacgccg aaatgcaggt tggacccgct
     3241 gcggtcttct cttgagaaat ctcccctgtt cttcatgtag gacttgtttg agggcgcaag
     3301 gtctgcagat cctcctataa ggttgggtat gcgggcggca agcttgttca gcacctcgcc
     3361 ggaggagttc cttgtagcat tcgccttgct gtcatagctc cagaagtctt catcatcgag
     3421 gagggtcccg gagtagtcct tctccttcca tgcctcccat tccgccgcaa gctccggata
     3481 ttccttctta tatgcttcca tcattttgtt ccattcgttt tcagccttca tgccgttggc
     3541 gcggatctgt tccatatacg ccttcacttc atccggcaca tggaattctt cggtgtaatc
     3601 ccaaccgagg gttttcttgg tctcaagcac attttccgcg ccaaggggct cgccgtgggc
     3661 tgatgccatg ccctgcttcg ggctgccgta cccgatctgc gtcttgatct cgatgaggga
     3721 aggcttgtcc ttcactgcct ttgccgcctt tatcgccgcg tcgatgtctt cgatgctggt
     3781 gccgtcttca actttcagag tatgccagcc gtaagcctcg aaacgctttc tgacatcttc
     3841 gcggaaggct atgtcggtgg aaccttctat ggtgatgctg ttggaatcat acagcactat
     3901 aagtttgcca agcccaaggg tccctgccag tgacgccgcc tcggaagcca cgccttccat
     3961 catgcagccg tcgccagcaa gcacgtaggt gtaatgatcg acgacagggt aaccggggcg
     4021 gttgaacctg ttggcaagat aagcttccgc catagccatt ccgacagcgt ttgcaagccc
     4081 ctggcccaga ggcccggtgg ttatctcaac gcccttggta tggccgtatt ccggatgtcc
     4141 cggcgtcagg ctgtccagtt gcctgaagtt tttaaggtcg tcgatggtca gtccgtagcc
     4201 gaacaggtgg agcagcgaat aaatcagcat cgatccgtgt ccggcagaca atatgaaccg
     4261 gtctctgtca tcccagccgg gatttgcggg gttgtgcttc agattccttg cccagagtgt
     4321 atatgccatc ggagcggctc ccatcggaag tccgggatgg cccgagttgg ctttctgtac
     4381 tgcttcggca gacaggatcc ttatggtgtt tactgtaaga agttcaacgt tgttcatatt
     4441 tacctcctgg catctaagat ttttattggt gcaaacccgc atgcccttcg gggtacgttt
     4501 cccttcggat tcatgcagcg cgattatata tttatatcaa acggttcact tcctgggctc
     4561 cgcccatatc cttgccttga agcagggtct tccgtcttcc tcgttcagtc cttctatata
     4621 gaccgtgttc ccgccgtcac gcggtatctc acggcagagc agcagcgtat cgccggggaa
     4681 cgcttcctta atataattta cttccatcgc ttttatcatg tactgcttat ggctttcgac
     4741 cgaaaaacag tccatgatgt agtctatata tctcgagttg tttatatgac cgttgaaatc
     4801 cacatcactg tagcccacag ttttcctgta gaccggctcg ggcttaaatg aaggcctgag
     4861 tttcccgggt cttgagtcaa gcgcatgtcc ctcagtgtac tcgggataat ccatgtggat
     4921 ataatcgctt tttcttagct ccctggtttt cgtatctatt atgacccata tggacttggc
     4981 cctgataatg gcatccccgt tcctgtcgca aaccctgaaa tccctttcga actcgatctt
     5041 tcccggcgga tggggccagg tcgcaacagt tatgctctca ttcaacccag ggatcctcgt
     5101 gaactccacc cttatgcggg tcagtaccca tgccgcggag tatttctccg ccagcgtacc
     5161 cactcctgcg ccaagcctgt ctaccgcctc actggccgtg tcctggaagt attcgaacag
     5221 tttgctgcac ttcaggtatt tcatgaaatc gacatcgctg tatccgatcg tatatgtgtg
     5281 tccgtatacc gcctctgttc ccaaatccga acctcctgca gcccatcctt cctttatact
     5341 gtcaaaaaag ataatagcac aacgcctcaa tattttccac tgataacatc aaatcgccga
     5401 gaaattttat aaaaatcagc acagatatct gcaatgatct gcatccgagt gtccaaaacc
     5461 tgcaaaaccg catattgata acaccgggct gagtaacagg aaattttgaa atggagaaag
     5521 ctactggaaa gggtttctcg cattgcccgc aggagccgca ttcctggcat tctccatgct
     5581 ggtatggcaa ttcggcgtcc gtcattacaa aagcaccgga agctgaactc ccggtgcttt
     5641 tgctccttat tgcagcattt ctgtctccgg ctgataatgc ttttttccgg caatatttat
     5701 cagaagctgt aacagctaca tagtttcact ggtgcactct actccgctgc cttcctcaat
     5761 gcctgctcca ggtcataaat caggtcttcg ggatcctcga tgcctatcga aagcctgatc
     5821 tggtccggcg tcacacctgc ggccttctgt tcctcaggag acagctgggc atgtgtcgtg
     5881 gacgccggat gtatcacaag ggacttcgcg tctgcgacat tggcaagcag cgagagtatc
     5941 tcaaggctgt ctatgaactt cactgcagcc tcgagtccgc cctttatgcc gaaggtgaag
     6001 atggagcctg ccccctttgg catgtacttc cttgcaagct cgtggtactt gttgttctca
     6061 agacccggat agttgaccca tgcgacctgt ggatggccat ttaagtattt tgctatcttc
     6121 atcgcgttat ccacatgtct tgccactctc agcgacagtg tttccagccc ctgcagcagc
     6181 aggaacgcat tgaacgggct gagggcggcg ccggtatccc tgagcagctg cacccttatc
     6241 ttcgttacat aagcagcagc accaacatct cttgcgtaaa cgatgccgtg gtagcttgga
     6301 tcgggctcgg tcagccccgg gaacttcccg cttccgatcc agtcgaattt gccgccgtcg
     6361 acgatcacgc cgcccaagct ggtgccgtgg cc
//
LOCUS       DAOSCC010000142         6246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7981_length_6246_cov_3.641576, whole genome shotgun sequence.
ACCESSION   DAOSCC010000142 DAOSCC010000000
VERSION     DAOSCC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7981_length_6246_cov_3.641576"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLG72_08110"
     CDS             complement(<1..640)
                     /locus_tag="PLG72_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80798.1"
                     /translation="MKKNNRSTTVIAILVFAAAMLCLLGAGTVSGKISLLPSLEAPGG
                     SNTPDELSGLQSRDKIPGQQLPGTIPDAQQPGISPGLEIPDTPAGLPSPETASSLTGS
                     VQDETASQQPGQYAGNSAGLLRCLHYTEKDFKKFVNGTSPAYDTLFSINKGAGTGAIM
                     GGIVPHHSVAGRLIAEFFRALAEDPPETVIVIGPNHKLEGSGEIQTNPTDWGT"
     gene            complement(793..5793)
                     /locus_tag="PLG72_08115"
     CDS             complement(793..5793)
                     /locus_tag="PLG72_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ80799.1"
                     /translation="MGKIKELFRKKTAIIITAAVLLFAAAAATAALTGLFGGIGKSAG
                     TTAGADTGTDTQKDGLSDAIRYAELLDSIVEISPTDQDSLGVSTTSAFRLLFSRDTDE
                     KAIVSTLSVEPKQDFSINRVSGNEFSLAFDKPMESNKIYTFTLNDMDNGEKKSWAFQT
                     KKQLNVVRTLPRDRAVYVPVNSGIEITFSHSGIENAEECFEITPKVNGRFEWNKKTLV
                     FVPENLEYDTIYTVTVKKGIRVKGSSETLQDDYTFKFQTEQKPTSKMYFQFSDNFYNF
                     IPGSVPALEVYTMGEMAGKEVPVEIYSYPNAPAFMDALKEAQSRPYWAVKDSNVDYGV
                     TKLEKVASLNCTVTEQGANYWNRYYLILPSTLQEGYYLAAVDIDGDKYYAPFQINGTS
                     VYIMTANNKYLAWLNDAITGQPISGAVFRHESGASAVSDNKGLAVFDAPKNNSDTAYD
                     FFMIEPGSKLPAVACVTNYSTIYYWYNNYYGSAITDNYWTYMYLDRSVYLPRDTVNVW
                     GIIKPRDGADIGNEAVLELIGYNWNFPDSEEPPVITSQSIFLSPDGTFTGSLRISNFN
                     PGGYNIRVRIGDKIMASQYISVQEYTKPIYTVDVVPDRKYMFGWETVNFDITSSFFEG
                     TPASGMKLDYSAMISYLYGTKETGAVTSDSSGKSSVSVKPASKSTGWKPETMEFMVTN
                     SDAEEQQIRENQYIYVFPRDTMVEVGTKTENGKGTISFTTSRIDLSGLKSNGSGYPSV
                     DDYRGESVDMPVKAKLYERYYKKVKTGDYYDHINKVRRDIYEYREVENLVSEYSFTTV
                     GGKYEVQFNAEKDKHYKLVVYAADSRGRSIEDTTYIYNWDSYDPYNTSKYILKQPDVS
                     RYYRTGETVKAEVTYNDDEPFAGENRKYLFIRLRNGIIDHTVSEDALYEFPFESRFIP
                     NIYVKALCFDGTGIYDAGIINFRYESSEKKLDINITTDKQSYRPGEEVKLSLDVKDSS
                     GKPAAAEVNISIVDEAYFALYGQNADILGGLYNSSLSSGLLAEYLSYKPYEEGMDGGA
                     EMGGEGGDSSTRKDFRDTALFTAVKTGADGKAEVSFRLPDNLTSWRVTCQAVTSDIKA
                     GTRVINVSSKLPFFVDSIFNKSFMKGDSPSILVRANGEELPEGAEVGFKVTVTAEDGT
                     SKDFTAKGIAKLHTEIQLGPLDTGNYTIRIEGSYGSLKDAMERSFRVSDSLLETSVTD
                     FIPLAEGVSLATDVKGLTTLTFFNEDSLVLYNELYSLFYNWGVRLDQVLARKISSKLL
                     KNYFNEEPYYDEDFDLSKYQTDDGGLALLPYDSSSPEVAAKMCALAADSIDRGALAAY
                     FYGLIENENTVPEDAVYAYWGLAALKEPVLLDIRSLLSEDGLDMKTRLTLGVALAEAG
                     DYQGAARIYNEFMSSRGTVTETYAWLDAGTRDDSIDATALCTLIAMKTNAPEKMKLFN
                     YISSNSTSMLLVNMERMMFVTNFIKDASLTGSFTYELDGVKKQVGLNRGSSFRLILTP
                     EKLSSIKFSNITGNIVAARSYTAPISEVMKTAGDIVSISRTFEKAGTKGPADTFDRSD
                     TVRVTLTLHFSENAPDGYYEVTDILPAGFRYTRVSYSDKDIGINEKWHYPYEVTGQKT
                     VFGCYYNKKYDKNEYSIIYFAKAVSPGTYTADSAAVRHTDSDIAGFSQTDRITVNK"
BASE COUNT         1260 a         1620 c         1584 g         1782 t
ORIGIN      
        1 gcgtgcccca gtccgtagga ttggtctgga tctcgccgga tccttccagt ttgtggttcg
       61 gacctataac gatcactgtt tcgggaggat cctccgcaag tgcccggaaa aactcggcga
      121 tcagcctgcc ggcaacggaa tggtgcggaa cgataccgcc catgatagcc cctgtgcctg
      181 cacctttatt tattgagaac agtgtgtcgt aagccggcga tgtcccgttg acgaactttt
      241 tgaagtcttt ttccgtgtaa tgcaggcacc tgagcaatcc cgcactgttt cctgcatatt
      301 ggccaggctg ctgtgaagcc gtctcatcct ggacactgcc ggtcagtgac gaggctgttt
      361 caggggatgg aaggccggcg ggcgtatcgg ggatctccag tcccggggat atgcccggct
      421 gctgcgcatc cggaatggta ccgggcaact gctgacccgg aatcttatca cgtgactgca
      481 ggccggaaag ctcatcaggc gtattcgaac cgccaggcgc ttcaagggac ggaagcaggc
      541 tgattttacc cgataccgtg cctgcgccaa gcaggcacag cattgcggcg gcaaacacta
      601 atatcgcaat tactgttgtg cttcggttat tctttttcaa cctgccttcc cccattcaaa
      661 gccggaatgt tttccattcc ggctgttcat cgaatcccgg ccacggactt aaagaccccg
      721 gttccggcct gcagttcgcc gccgtcatcg taattactgc catacgctgc tggttttatt
      781 tactgccgta tgctacttat tcactgttat ccggtcagtc tgcgagaagc ccgctatatc
      841 gctgtccgtg tggcgtacgg ctgcgctgtc ggccgtgtat gtgcccggtg agaccgcctt
      901 tgcgaagtat atgatgctgt attcgttttt atcatatttc ttgttgtagt aacagccgaa
      961 tacggtcttc tggcctgtca cctcataagg ataatgccat ttttcgttta tgcctatatc
     1021 cttatcggaa taggaaaccc tggtgtacct gaagccggcc gggagtatgt cggtcacctc
     1081 gtagtagccg tcaggcgcgt tctcgctgaa atgcagagtc agtgtcaccc tgacggtatc
     1141 cgacctgtca aatgtgtctg ctggaccttt tgtaccggct ttttcaaagg tcctggatat
     1201 cgatacgata tctcccgcgg ttttcatcac ctcgcttatc ggcgccgtat atgagcgggc
     1261 tgccacaata ttgccggtga tgttgctgaa ttttatagag gacagtttct caggggtgag
     1321 tatgagcctg aaactgcttc ccctgttcag cccgacctgc tttttcactc cgtcgagttc
     1381 atatgtgaag ctgcctgtaa gtgacgcatc ctttatgaag ttcgtcacga acatcatcct
     1441 ttccatgtta acgaggagca tggacgtgga gttggaactg atataattga acagcttcat
     1501 tttttcagga gcgtttgttt tcatcgctat caatgtgcac agcgccgttg cgtctatact
     1561 gtcgtccctt gtacctgcgt caagccatgc gtaggtttcg gtgacggtcc ccctggaaga
     1621 catgaactcg ttgtatatcc gggcggctcc ctggtagtct cctgcttctg caagcgcaac
     1681 gccgagcgtc agccttgttt tcatatccag cccgtcttcg gaaagcagcg acctgatatc
     1741 gagcagcaca ggctccttaa gtgccgcgag cccccaatat gcgtataccg cgtcttcggg
     1801 aacggtattt tcgttttcga tcaggccgta gaaataggct gcaagggcgc ccctgtctat
     1861 gctgtcggct gccagtgcgc acatctttgc agccacttcg gggctgctgc tgtcgtaggg
     1921 caggagtgcg aggccgccgt cgtccgtctg gtacttcgac aggtcgaaat cctcatcata
     1981 gtacggttct tcattgaagt aattcttaag cagctttgac gagatcttcc ttgcgagcac
     2041 ctggtcgagc cgcacgcccc agttatagaa cagcgaatac agttcgttgt agaggaccag
     2101 cgaatcctcg ttgaagaatg tcaaagtcgt caggcctttt acgtctgttg ccagagatac
     2161 tccttctgcc agcgggatga aatcagtcac cgatgtctca aggaggctgt cgctgaccct
     2221 gaagcttcgc tccatggcat ctttcaggct gccatacgag ccctcgatcc ttatggtgta
     2281 attgccggta tcaagcggtc ccaactggat ctctgtgtgc agttttgcaa tgccttttgc
     2341 tgtaaagtcc tttgaagtcc cgtcttctgc cgtgacggtg actttgaacc caacctcggc
     2401 tccttcagga agttcttctc cgtttgccct tacgagtatc gacgggctgt ctcctttcat
     2461 gaaggacttg ttgaatatgg aatccacgaa gaacgggagc ttcgaagaca cgttgatcac
     2521 cctggtgccg gccttgatat cgcttgtcac ggcctggcag gtgactctcc aggaggtcag
     2581 gttgtcaggc agcctgaacg atacttcggc cttgccgtca gcgccggttt tgactgccgt
     2641 gaacagggcg gtatccctga aatctttgcg tgtactgctg tcgccgcctt ctccgcccat
     2701 ttcggccccg ccgtccatgc cttcctcgta gggcttgtat gacaggtact ctgccagcaa
     2761 gcccgaagac agggatgagt tgtacaggcc gccaagtata tcggcgttct gtccatacag
     2821 tgcgaaataa gcttcatcta ctatgcttat attgacttcg gcggctgcgg gtttcccgga
     2881 gctgtctttc acatccagcg acagtttcac ttcttcaccc ggcctgtagc tttgcttatc
     2941 cgtcgttatg tttatgtcca gctttttctc gctgctttcg tacctgaaat ttatgatgcc
     3001 ggcgtcatat atgccggtgc cgtcaaaaca cagggctttg acataaatgt tcggtatgaa
     3061 cctgctttca aacgggaatt catacaaggc atcttcggaa acggtatgat ctatgatgcc
     3121 gttccgcagc cttatgaaca ggtatttcct gttttccccg gcgaagggct catcgtcatt
     3181 gtatgtcact tccgctttta ctgtttcgcc ggtcctgtaa tacctggaaa cgtcgggctg
     3241 tttcagtata tatttacttg tgttgtacgg gtcataggag tcccagttat agatgtatgt
     3301 cgtatcttcg atggagcggc cccgggagtc ggctgcataa accaccagtt tgtagtgttt
     3361 atccttttcg gcgttgaact gcacctcgta cttgccgcct actgtggtga aactgtattc
     3421 ggatacgagg ttctccactt ctctgtactc ataaatgtcg cgtcttactt tgtttatgtg
     3481 atcgtaatag tcgcctgttt ttactttttt ataatacctc tcatatagtt ttgcctttac
     3541 aggcatatcg acgctttcac ccctgtaatc atcaactgag ggatagcccg atccattgct
     3601 tttcaggcct gaaagatcga tcctgctggt ggtgaaggat atggtgcctt tgccgttttc
     3661 ggtttttgtg ccgacctcga ccatcgtatc tctcgggaag acgtaaatat actggttttc
     3721 tctgatctgc tgctcttctg cgtcactgtt ggtcaccatg aattccatcg tttcaggttt
     3781 ccatcccgtg ctcttcgatg ccggcttgac ggatacggag gattttccgc tgctgtcgct
     3841 tgtaactgcg ccggtctctt tggttccata caagtaggat atcatcgcgc tgtagtcgag
     3901 tttcatgccg gaagccgggg tgccctcgaa gaaggaggag gtgatatcaa aattgactgt
     3961 ttcccatcca aacatgtatt tgcggtcagg taccacatcc accgtgtata tcggcttggt
     4021 gtactcctgc actgaaatgt actggctggc catgatcttg tcgccgatcc ttacgcggat
     4081 attgtatccg ccgggattga aattcgagat cctgaggctt cccgtgaagg tgccgtccgg
     4141 cgacaggaaa atgctttgcg atgtgatcac cggaggttct tcactgtccg ggaagttcca
     4201 gttgtagccg atcagttcga gtacggcttc attgccgatg tcggctccgt ccctcggctt
     4261 tatgatcccc catacgttga cggtgtcccg gggaaggtac acgcttctgt cgagatacat
     4321 gtaagtccag tagttatctg tgatggccga tccgtaatag ttgttgtacc agtaatagat
     4381 cgtgctgtag ttcgtcacgc atgcgacagc cgggagtttc gatccgggct cgatcatgaa
     4441 gaaatcatat gctgtatcgg agttgttctt tggtgcgtcg aatacagcaa gccctttatt
     4501 gtcagacact gcagaagctc cgctttcatg cctgaaaaca gcgccggata ttggctggcc
     4561 tgtaatcgca tcattcagcc atgcaaggta tttgttgttc gccgtcatga tatataccga
     4621 ggtgccgttt atctggaaag gagcatagta cttgtcaccg tcgatgtcga cagccgcaag
     4681 gtaatatccc tcctgtaaag tggaagggag gatgaggtag tacctgttcc agtagtttgc
     4741 tccctgttct gtgacggtgc agttcagcga agcgaccttc tcaagctttg tcacgccgta
     4801 atccacgttg ctgtccttca ccgcccagta tggcctggat tgcgcttcct tcaacgcgtc
     4861 catgaaagct ggcgcattcg ggtaactata tatctcgacg ggcacttctt ttcctgccat
     4921 ttcccccatg gtgtatactt caagcgcggg gaccgagccc ggaataaagt tatagaagtt
     4981 gtcggagaac tggaaataca tcttcgatgt gggtttctgc tcggtctgga atttgaacgt
     5041 gtaatcgtcc tggagcgttt cgctgctgcc ctttacacgg atgccttttt tgaccgtgac
     5101 ggtgtatatc gtgtcgtatt ccaggttttc cgggacaaag accagcgtct ttttgttcca
     5161 ttcgaagcgt ccgttgacct tgggcgttat ctcgaaacat tcctccgcat tttcgatgcc
     5221 gctgtggctg aaagtgatct cgatgccgga gttgaccggc acatagaccg ccctgtcgcg
     5281 gggcagcgtg cggacgacgt tcagctgttt tttagtctgg aaggcccatg actttttttc
     5341 gccgttgtcc atgtcgttca atgtgaaggt gtagatcttg ttgctttcca ttggtttgtc
     5401 gaatgccagg ctgaactcgt ttcctgaaac cctgtttatg ctgaaatcct gcttcggttc
     5461 cacgctcagc gtcgagacaa tggccttttc atcggtatcc ctgctgaaca ggagcctgaa
     5521 tgccgatgtg gtgcttacgc caagactgtc ctggtctgtt ggggagattt cgacgatcga
     5581 atccagcaac tccgcatacc gtattgcatc ggaaagaccg tccttttgcg tgtctgtgcc
     5641 cgtatctgct cctgcagtcg taccggcgga tttcccgatg ccgccgaaca aacctgtcag
     5701 cgcagccgtt gccgctgccg ccgcgaacag gagcacagcc gccgtgataa tgattgcagt
     5761 ttttttgcgg aacagttctt tgattttacc catacatctc aaccttacct ttcattccgg
     5821 cccggccggt gataacaaaa atgctgtcat cgtattacaa tatacagacg tggcagcaga
     5881 tggttttgtt tcaaaatatt atatcttata tcgttccaat gatcaacggc agaacaatac
     5941 cgtcatcttc gccaagcccc ggtgctccct gctcatcata gttccaggaa aacaggtttc
     6001 ctgtttcaga taatgcggag cattctttgt ctccggcttc gattttgctg atagcttcgg
     6061 ggagcggatg tgttgcagat acgctgctat tgtatcacaa acttggaaat atgtatattt
     6121 atattgtgtc atactgcgat ttcagcggta cccataagcc gaaaaactgc acagataaga
     6181 agggaaatac ggtaaggggc ccggttattg cccaaaagct gtaaaaccgt acaaaatgga
     6241 attcgg
//
LOCUS       DAOSCC010000143         6245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_7983_length_6245_cov_3.907431, whole genome shotgun sequence.
ACCESSION   DAOSCC010000143 DAOSCC010000000
VERSION     DAOSCC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7983_length_6245_cov_3.907431"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1117
                     /locus_tag="PLG72_08120"
     CDS             341..1117
                     /locus_tag="PLG72_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HOJ80800.1"
                     /translation="MKDKILAMIRKRLPIILVTIGVLLMAYPVIGRIYARYQEEKMLA
                     EWLNSIDVSDFGDADAADPEESYALLQDAFDTEDSGNGGKGTGISDNAGGDGGQTGDG
                     GASAGNGTGQKSSASDLSNQRVLGIIQIPKIKVKDPIVEGVEKSNLKAGIGHVPGTPL
                     PGQPGNCALAGHRNYALKKLFRRLDELEAGDEVIIITKTETLRYTVTGKVVVEPDDVS
                     VLQGSKDKSIISMITCTPMYVHSHRLVVFAELVERVPREP"
     gene            1565..4206
                     /gene="alaS"
                     /locus_tag="PLG72_08125"
                     /pseudo
     CDS             1565..4206
                     /gene="alaS"
                     /locus_tag="PLG72_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
     gene            4283..4627
                     /locus_tag="PLG72_08130"
     CDS             4283..4627
                     /locus_tag="PLG72_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOJ80801.1"
                     /translation="MQDCIFCKIIERQLPSTVVYEDDKVMAFNDINPVAPIHVVIIPK
                     RHIANVNELTEENASMVGDIHLAAKKIAEKLGVADKGYRLINNCGPDAGQTVFHLHYH
                     LIAGVKLGAKII"
     gene            4757..5770
                     /gene="galE"
                     /locus_tag="PLG72_08135"
     CDS             4757..5770
                     /gene="galE"
                     /locus_tag="PLG72_08135"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOJ80802.1"
                     /translation="MAVLVTGGAGYIGSHTCVQLLEAGYDIVVLDNLCNSKIEAVRRI
                     KELTGKDFVFVQGDIRDSDLLDDIFSKYDIDSVVHFAGLKAVGESVSVPLRYYRNNVT
                     GSILLFESMQRNGVKNIVFSSSATVYGMAEKMPLREDAPLGAVNPYGRTKLMIEDILR
                     DIYSADNSWNIILLRYFNPVGAHPSGRIGEDPNGIPNNLMPFISQVAVGKLKELNVFG
                     NDYPTPDGTCIRDYIHVMDLADGHVKALEKIKENPGVVAYNLGTGKGSSVMEMIAAFA
                     EATGIKIPYKITGRRPGDAPVSYADPSKAEKELGWKASRNIIDMCRDLWTWQSQNPDG
                     YGD"
     gene            5948..6124
                     /gene="rpsU"
                     /locus_tag="PLG72_08140"
     CDS             5948..6124
                     /gene="rpsU"
                     /locus_tag="PLG72_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ80803.1"
                     /translation="MSEVRVKENESLDSALKRFKRQCAKAGVLAEVRKREHYEKPSVR
                     RKKKSEAARKRKHR"
BASE COUNT         1814 a         1309 c         1811 g         1311 t
ORIGIN      
        1 atatagtcgg ctgggcttcg ggtaagctgg ctgagacagg tggagttgct gcagaagttt
       61 tctatgcagc aggcggcctg ttcatagttg ctgccggcgt gcttgcagct gcaaggaaga
      121 agtaagccac aggtttgttg ccaggtacat aagatgagtg ccaaaattgc tgatacttga
      181 acatatggaa atagcaaata agagaatata ttggatgatc aggatcatat ctgataatat
      241 gatgtgtgat tgtagtattg gcagaaagct gtttgaggat tcctgagcaa atcaggcgtc
      301 cttttttatt tgctgagagc atacaaacca agaggtgcat atgaaagata aaatacttgc
      361 aatgatcaga aaaaggctgc caataatttt agtgacgata ggcgtcctgc tgatggccta
      421 ccctgtaatt ggcaggatat atgccaggta ccaggaagaa aaaatgctgg cagagtggct
      481 gaacagcatc gatgtaagcg attttgggga tgctgatgcc gcagatccgg aggaatccta
      541 tgcacttctc caggatgcat tcgataccga agacagcggg aacggcggca agggaaccgg
      601 gatatccgat aatgccggag gagacggagg gcagacaggg gacggaggag cgagtgccgg
      661 aaatggtacg gggcaaaaat cgtcggcgtc ggacctgtcc aatcagagag tgcttggtat
      721 tatccagatt cctaaaataa aagtgaagga tcccatcgtt gaaggagttg aaaaatcgaa
      781 tctgaaagcc ggaatcggac atgtgccagg gacacctctg cccgggcagc caggcaactg
      841 tgctctcgcg gggcacagga actatgcgct gaaaaagctg ttcagaaggc tcgatgagct
      901 tgaggcaggc gatgaagtca taatcatcac gaaaaccgag acattgagat atactgtaac
      961 gggaaaggtc gtcgtggaac ctgacgacgt gtcggtactc cagggaagca aggacaaaag
     1021 catcatatcg atgatcacat gtacgcctat gtatgtacat tcccacaggc tggtcgtttt
     1081 tgcggagcta gtggagcgcg taccgaggga accttaaatg gactgaaact tgcttgcaga
     1141 gttaaaaacg gcggcttaat cactgaaaaa atatggcgct gacacatgcc atgccagcgc
     1201 tatttttaat aggaatcctg taacaattac tactttactc gtaccaatat tactttgaac
     1261 ggcacgggac atgttgttgc cacgcccctc tttataacgg gatcacctga gaacgatgcc
     1321 gaaaactgaa atgaatgcaa tggtccgcag gaatcctgcc atcctgaaag aatgatttga
     1381 atccgggcat tcaattccct gcaattcctt gacagttccg gcctgctttt atattataat
     1441 tttaattatc cagcagaatt ttagctgtat tgtcactaaa ctattaaaat aatcgcataa
     1501 gtcgtctata attaaactat aaaataaatt tacgcgcaat gataaaggat agaggatgga
     1561 caacatgaaa aaactgggac tgaatgaaat aagggagaag tatttaagtt tctttgagag
     1621 caagggccat ttgcgcctgc ccagcttttc gctggtaccg aagaacgacc cgagcatact
     1681 gctcataaat gccgggatga cgccgctcaa gccgtatttc acgggagcgg agaagccgcc
     1741 gcgtacaagg gtgacgacgt gccagaagtg tataaggacg ccggatatag aaaatgtagg
     1801 gaagactgca aggcacggca ctttcttcga gatgctgggc aatttctcgt tcggcgacta
     1861 tttcaagaaa gaagcgatca catgggcatg ggagtttgtc acgaaggtca tggaaatgcc
     1921 ggaggacagg ctctatgttt cgatttacga agaagacgat gaagcgttcg agatatggaa
     1981 caaagaagtt ggcctggcgc ctgagcgcat cttcaggatg gggaaaaagg acaacttctg
     2041 ggagcacggc accggcccat gcggaccatg ttccgagatc tatttcgaca ggggccccga
     2101 gagaggctgc ggaaagcctg actgcacggt aggctgcgac tgcgacaggt atatagagtt
     2161 ctggaacctc gtatttacgc agttcgataa acaggaagac ggcacttata caaagctgga
     2221 gaagaagaac atcgataccg ggatgggact ggagcgcctg gcagttatca tgcaggacgt
     2281 tgacagccta tttgaggtgg atacgataag gcgcatactg gagtatgtat gcgagaaagc
     2341 cggcgttgtc tacggccatg gcgacgagga cgtgtccatc agggtcataa ccgaccatat
     2401 aaggagcacc acgatgatgg tcgcagacgg gatacttcct tccaatgaag gacgcggata
     2461 tgtcctcagg aggctactca ggagggccgc aaggcatggc agactgctcg gcatcgaagg
     2521 gccgttcctg tatgatgtgg cgagagtcgt tataagggaa tcctgtgagg catatcctga
     2581 actggctgaa aaggaagaat acatctgcaa ggttataaag atagaggaag agcgctttgc
     2641 ggctaccatc gaccagggca tagcaatact caatgccatg atcgcagagc tgaagatgga
     2701 aaacaggacc gtactgccgg gaagcatggc cttcaagctg catgatactt acggcttccc
     2761 gctggacctg acaagggaga tcgcagagga agccggcctt acagtcgacg agcagggatt
     2821 catggctgag atggcggagc agaaaaacaa ggcgcgtatg gcaatcaaga gcaaggagat
     2881 atctgcatgg ggccgcgata ttgcggcagg gattgacaag gatctcagga ccgagttcgt
     2941 cggatatgag agctattcgt ccgaaagcaa aatactgtat atcatcctta acgacgagca
     3001 ggcggacagt gcgcagcagg acgatgaagt cacgatcata ctggacagga cgccgttcta
     3061 tgctgaaagc ggcggccagg tggcagatac cggcatgata accggaagag acggaaaggt
     3121 caggatcacc ggctgcgtca agaccggcga aggtttgtat ctccataccg gcgttgttga
     3181 ggaaggcata atagagaaag gttctgcagt gactgcggcg atagatattg aaagaaggct
     3241 tgccactgca aggaaccaca cggcgacgca tcttctgcac aaggcattga ggaatgtgct
     3301 gggcgatcat gtgacgcagg ccggatcgct ggtcgagcct gacaggctga gattcgactt
     3361 ccaccatttc tccgcgatgt caaaggaaga gataaagcgg gtggaagatg aagtaaatgc
     3421 caggatactt gatgatctca atgtgaacgt aagggaaatg cccattgaag aggcgaaaaa
     3481 gctcggagcg atggcgcttt ttgaagaaaa atacggagat gtcgtcagag tcgtacaggc
     3541 aggcgattac agcacggaac tctgcggcgg cacccacctg aaatcgacgg cccaggcggg
     3601 actcttcaag ataatcagcg aaagcggagt cgcggccggt gtaagaagga tagaggctct
     3661 tacaggcaaa ggcgcgctgg aatactatgg ccatgtggaa gagaagctgg aaaacatcgc
     3721 gtcggtgctg aaaacgacgc cgcaggagag cctgagaagg gtggagacca tcagtgcgga
     3781 actgaaagcc gccctgagga gatagaacag ctgaaggcaa aactggcaag cagttcgttc
     3841 gacgaggtgc ttgcaggcgc ggtggatgtc aacggaatca aaattgcgac aggcaggttt
     3901 gatcagcttg atatggattc gctgcgcaac acctgtgaga acctcaggag cagggtcggc
     3961 tccggagtcg tggtcttggg atcggctttc ggggacaagg taagtttcgt ggcaatggcg
     4021 acgaaggatg ctgtcgaacg gggcgtccac tgcggcaata tcgtgaagga agcggcaaag
     4081 gcagcaggcg gaggcggagg cggaggcggc aggccggata tggcacaggc cggcggcaag
     4141 gatacatcga agatcgacaa agcgctcagg cttgcggtcg atgctgccag ggcacagata
     4201 aaataattgt ttgcaaatta taaaaagctt gcaatttata aaatggatat aactcataaa
     4261 ataaacggag gaaggtgcag cgatgcagga ttgtattttt tgcaaaatca tcgaaaggca
     4321 actgccgtca acagttgtat acgaggacga taaggtgatg gcgttcaatg atatcaatcc
     4381 ggtggctccg atccatgttg tcatcattcc taagaggcat atagccaacg tgaatgagct
     4441 tacggaggaa aacgcttcaa tggttggcga tatccacctt gccgcaaaaa agattgcgga
     4501 gaagctcgga gtcgcagaca agggctacag gctgataaac aactgcggcc ctgatgcggg
     4561 gcagaccgtg ttccatctgc attatcacct gatcgcagga gtcaaactgg gagcgaagat
     4621 aatctgaggc atcaggcgtt ggagaacgga tgccaaagac aagatatcag gtaccagata
     4681 ctaagggaaa gataaaaacc acagatcata atcagtgtaa aaccttaagg ctatactcag
     4741 aaggagagcg tgttatatgg cagtacttgt taccggcgga gccggatata tcggaagtca
     4801 tacctgtgtc cagctgcttg aggcaggata tgatatcgtt gtgcttgaca acctttgcaa
     4861 cagcaagatc gaggctgtca gaaggataaa agaacttaca ggaaaagact ttgtatttgt
     4921 gcagggagat atccgcgaca gcgatctgct ggacgatatt ttcagcaaat acgatatcga
     4981 ctcggtagtg cattttgccg ggctgaaagc tgtgggagag tctgtgagcg taccgctgag
     5041 gtattaccgc aacaatgtga ccggctcgat actgctgttt gagagcatgc aaagaaacgg
     5101 cgtgaaaaac atcgttttca gttcatcagc cactgtctat ggtatggcgg agaaaatgcc
     5161 cctgcgcgag gatgcgccgc tgggcgcagt caacccgtat ggacgtacca agctgatgat
     5221 agaagatata cttcgtgata tttattctgc cgacaacagt tggaacataa tactgctcag
     5281 gtatttcaat ccagtgggcg cacatcccag cggaaggatc ggcgaagatc ccaatggaat
     5341 acccaataac ctcatgcctt ttatttcgca ggtcgctgtg ggcaagctga aggaactcaa
     5401 tgttttcggc aatgattatc cgacgccgga cgggacatgt atcagggact atatccatgt
     5461 gatggatctg gctgacggcc atgtgaaggc tctcgaaaaa ataaaggaaa acccgggcgt
     5521 cgttgcatac aatctgggca ccggcaaagg ctccagcgtt atggagatga tagccgcgtt
     5581 cgccgaagcc acaggcataa aaatacctta caaaataaca ggcaggcggc cgggagatgc
     5641 tccggtaagc tatgctgacc cgtcaaaggc tgaaaaggag cttggctgga aagccagcag
     5701 aaatatcatc gatatgtgca gggacctgtg gacgtggcag tcgcagaacc cggacgggta
     5761 cggcgactga gacagccgtg cttccagttt gcggggtctt tcatgcgtcc cgtttctatt
     5821 gactttattt tgaaggataa tatataatat actgtgtgct gcacatagct tgattcccac
     5881 atgcaatcga agcaatatac gcggcttgta tacggcacaa tagctgccat cggagggagg
     5941 gatatacgtg tcagaagttc gggtaaaaga gaacgagtct ctcgacagcg cactcaagag
     6001 attcaagcgt cagtgtgcca aggcaggcgt tctggctgaa gtcagaaaaa gagagcatta
     6061 cgagaagccg agcgtcagaa ggaaaaagaa gtctgaggct gccaggaaaa ggaagcatag
     6121 ataatcctgc gcagttttaa gtgttcattt tttctcggga ggctggccga atgtccctca
     6181 aggaacagtt actggaagat atgaaagatg ccatgaagaa aaaggatacc gtcaggaaga
     6241 acgct
//
LOCUS       DAOSCC010000144         6190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8067_length_6190_cov_3.857213, whole genome shotgun sequence.
ACCESSION   DAOSCC010000144 DAOSCC010000000
VERSION     DAOSCC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_6190_cov_3.857213"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLG72_08145"
     CDS             complement(<1..834)
                     /locus_tag="PLG72_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80804.1"
                     /translation="MEKLGCLGGTATAGGINYFLGGRKLDEKSKKHIRVIGGIFDEIT
                     DKLISEGNAIEPDTIDLDFNPVGWYPRMASGIVFDELALKILMDDMCEALGIRVYYNS
                     ALIDVKRTGRFIDSVIIHNKDGLVSLEAGCFADCTGDADLAALSGCSCLKGRESDGLT
                     TPASLEMHVENVDGNELVRYQNEHQSPKLVEIIERLKKDGIWKFPYEIFVCMQLVHKD
                     VFLINTIRQVCVDGTSEASISKALAQGRKENLELFRIMKEYFPGFKNARIRKVCDFIG
                     IR"
     gene            854..1432
                     /locus_tag="PLG72_08150"
     CDS             854..1432
                     /locus_tag="PLG72_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80805.1"
                     /translation="MRAALTAAGYAVSKIRFRGKKLIFTLVIACLVMPKEIYLLPLYQ
                     QMVSYRWIGSMLGFIIPDIASPFGVYLMTNYYDTIPDEIYESASIDGSGHFRFFTDFA
                     LPLSKPGIAALGILTFIRTWNSYLWQFVMKTNENTYTLPVGIASLFDNPDIVDYGLKF
                     AGASITAIPLLLIFMLFQKFFVSGITSGSVKG"
     gene            1467..>2021
                     /locus_tag="PLG72_08155"
     CDS             1467..>2021
                     /locus_tag="PLG72_08155"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ80806.1"
                     /translation="MKNKERFGGIIPPLVTPLLPDETLDVVTTKQLIEYLIQNNVNGI
                     FVNGTSGEAMRVTESVWEGITRLALETVNGRVPVFCGAIDTSTLRVIERIRRIEAMGG
                     ELVVCTPPFYLKSFGQDEILRHYDKICSSVSKNIQIAVYNIPSTTGANIEPETIAKLA
                     DYDNIVAYKDSCADFEQVQHDLFQL"
     assembly_gap    2022..2031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2032..2395
                     /locus_tag="PLG72_08160"
     CDS             <2032..2395
                     /locus_tag="PLG72_08160"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ80807.1"
                     /translation="ADKDICMFNGAEDLCAASMLFGAQGCIPGLANFYPELFVRLYQL
                     CRKGEVAESVRLQEQISEIRKCLHVGTSWMAVMKYLLKREGFGHDTVSSPIPPLNDEH
                     RRSIDAILERAGVSRRER"
     gene            2392..3351
                     /locus_tag="PLG72_08165"
     CDS             2392..3351
                     /locus_tag="PLG72_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ80808.1"
                     /translation="MRAYGYLALSWVAHYAQRCPVAYQLLKSYGVEVKSTEELTQDEL
                     ERLLPEADFAIAGLNIWDETLFKKMKKLRLLTKFGSGVDNIDLVAARRFGVDVCNTRG
                     GNANAVAELAVGLMISVLRYIPQSNSEIHQGEWVKRTGSELRGKTLGLVGFGMIARRL
                     AELLRAFNMRIIACDPWPDRGEAERLGVELVTLQQLLQESDIISLHAPYTAETRGMIG
                     ANELAGMRDGAVLINCARAGLVDTPALAEALRSGKLAAAALDVFDEEPVSSSNPLIHM
                     PNVICTAHYGGSTFESMYIDSMISAQAIISVLRGEQPENLLNR"
     gene            3391..4398
                     /locus_tag="PLG72_08170"
     CDS             3391..4398
                     /locus_tag="PLG72_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ80809.1"
                     /translation="MIGFRPYFFISKCPGLKVNNMYIVSDRCKDISEKVKKYKNEILI
                     FLAISVALLGDASLYVLLPVHASDRGISIASLGVLLSVNRLVRLAVNSFIKYLHKWTG
                     SRTLFTASITLTALSTLIYGLPVSFITLLAARIIWGCCFSSMRYIAYLKIAESSDDSN
                     RGELTGLYNSAIRVGSLIAAPLGAALAGLLVSIRHFYTVFLSVYFGRLSDRYGRFRVV
                     VLSCCIQAVSLAVIALSRSFLLLSMAVLAAFTGATALTNTLSAAAMDCHHIYKTKNVL
                     GCFTTWFDIGSALGTMAGYTLMVAIGYSRTYIYSGLILTAVLGLIARKMFAGAEQANQ
                     N"
     gene            4518..>6190
                     /locus_tag="PLG72_08175"
     CDS             4518..>6190
                     /locus_tag="PLG72_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOJ80810.1"
                     /translation="MGSFFRTEGSRLIREYRNEILWIEPWGANSLRVRGTKRAAMEQQ
                     QDWALLPGPGGDAAEISISDDGRSAEIRNGKITAKIFPNGKLAYYNSEGRLLLEEYVR
                     SRDNLKEFVSALCINSREYSPVPGTNDFRLTVRFEANDDEKLFGMGQYQQPYLDLKGC
                     TLELAHRNSQASVPFLLSNRGYGFLWNNPAVGKVTFGRNVTEWYAGSVQQMDYWITAG
                     DTPAEIEEAYARVTGTVPMMPDYAMGFWQCKLRYQTQEELLEVAREYKRRGLPISVIV
                     IDFFHWTKQGDFKFDPEYWPDPEGMVRELREMGIELMVSIWPTIDKTSENFREMHEKG
                     YLVRFDRGNSIVMDWMGNTMFFDATHPGAREHLWKIIKRNYYDKGIRIFWLDEAEPEY
                     SEYEFDNYRYHIGTALQVSNIYPLMYAKTFYDGMKAAGQENIINLIRCAWAGSQRYGA
                     LVWSGDIHSSFRALRNQVRAGLNMAIAGIPWWTTDIGGFHSGDIRDPAFHELIIRWFQ
                     YGTFCPVFRLHGDREPHTPPMGTSGGGIFGSGAPNFFFFKQKTAYEIFK"
BASE COUNT         1542 a         1468 c         1596 g         1574 t
ORIGIN      
        1 acggataccg ataaaatcgc acactttccg gatccgtgca tttttgaagc ccgggaagta
       61 ttctttcatt attctgaata actccagatt ttcttttcgt ccctgtgcca gtgctttgct
      121 tatggatgct tcactggtac cgtcgacaca tacttggcgt attgtgttga taaggaagac
      181 atctttatgt acaagctgca tacatacgaa gatctcgtaa gggaatttcc agatcccatc
      241 tttcttcagc cgttcgatta tttcaaccag cttgggtgac tgatgctcat tctgatatct
      301 gacaagttcg tttccatcca cgttttctac atgcatttcc agtgaagccg gcgtcgtaag
      361 tccgtcagat tcgcggcctt taagacaact gcagccagaa agggcagcca gatccgcgtc
      421 tcccgtacaa tctgcaaagc agccggcctc aagcgatact aaaccgtctt tgttgtggat
      481 tatgacagaa tcaataaaac gtcccgtcct ttttacatca ataagggcgg agttgtaata
      541 gacacgtata cccaatgctt cacacatatc atccatcagg atcttcagcg ctaactcgtc
      601 aaatacgata cctgaagcca ttcttggata ccagccgaca gggttgaaat ccagatctat
      661 cgtatcgggt tcaatagcat ttccttccga aatcaattta tcggtaatct catcaaagat
      721 cccgcctatg acgcgtatat gctttttgct cttttcatca agctttcgcc cgccaaggaa
      781 gtaattgatc ccgcctgccg tcgcggttcc gcccagacaa ccgagctttt ccacaattgc
      841 agttttaagt cccttgcgag ctgcactgac agcagcaggc tatgctgtct caaaaatacg
      901 ttttcgcggc aagaagctga ttttcaccct ggttatcgct tgtcttgtaa tgcccaagga
      961 gatctatctt ctgccgctat atcagcagat ggtctcctac cggtggatcg gctccatgct
     1021 gggatttatt atcccggaca tcgcttcgcc gtttggtgta taccttatga ctaactacta
     1081 cgacaccata cctgacgaaa tttatgagtc ggcgtcgatc gacggcagcg gacatttcag
     1141 atttttcacg gattttgcgc ttccgttgtc aaagccgggg atagctgcgc ttgggatact
     1201 gacatttatt cgcacatgga acagctacct gtggcagttt gttatgaaaa ccaatgaaaa
     1261 cacctatacg ttgcctgtag gcattgcgag cctgttcgat aatcccgata tcgtggacta
     1321 tggtctgaag tttgcaggcg cttcaattac ggcaatacca ctgctgctga tcttcatgct
     1381 attccaaaag ttttttgtat caggaattac atctggatca gtcaagggct agcaagaaac
     1441 atatgagaca gaaagaggtt taaaacatga aaaacaaaga gcgttttggc ggaattattc
     1501 ctccgctggt cacaccactg cttccggatg aaacgcttga cgttgtcacc actaagcagc
     1561 ttattgaata cctgatccaa aacaatgtta acgggatatt cgttaacggt acttccggag
     1621 aagcgatgcg cgttaccgaa agtgtctggg aaggaatcac aaggctggcg cttgagaccg
     1681 tcaatgggcg tgtcccggtc ttttgcggag caatagatac gtccacgttg cgggttattg
     1741 aacgcatccg gcgtattgag gcgatgggcg gcgagctggt cgtttgcacg cctcctttct
     1801 acctgaagag ttttggacag gatgagattt tacgccatta tgacaaaatt tgctcatcag
     1861 tcagcaagaa catacaaatt gcagtttaca acataccatc gaccaccggt gccaacatcg
     1921 agccggagac catcgcaaag cttgccgatt atgacaatat tgtcgcttac aaagacagct
     1981 gcgctgattt tgagcaggtc cagcatgatc tgttccagct gnnnnnnnnn nggcagacaa
     2041 agacatatgt atgttcaacg gggccgagga tctatgcgct gccagcatgc tcttcggtgc
     2101 acagggatgt atcccgggac tggctaattt ttaccctgag ctgtttgtca ggctgtacca
     2161 gctttgccgg aaaggcgaag tggctgaaag cgtccgtctc caggaacaga taagcgagat
     2221 ccggaagtgc ctccatgttg gcacatcctg gatggcagta atgaagtatt tgcttaagcg
     2281 ggaaggcttc ggacatgaca ccgtttcttc accaataccg ccgctcaatg acgagcatcg
     2341 ccgtagcatt gatgctatcc ttgagcgtgc aggtgtctcc aggagggagc gatgagagca
     2401 tatggctacc tggctctgag ctgggtagcc cattatgcgc agcggtgtcc cgtagcatat
     2461 caactgctga aatcatatgg cgttgaggta aagtcgacag aagaactgac acaggatgaa
     2521 ctcgaacgcc ttttgcccga agctgatttc gcaattgccg ggctcaacat ctgggacgag
     2581 accttattta aaaaaatgaa aaaactcagg ctgctgacca agttcggctc aggggtggat
     2641 aacatcgacc ttgtggctgc acgccgcttt ggcgttgatg tatgcaacac caggggcggt
     2701 aatgccaatg ccgtggccga gcttgccgtc ggtcttatga tctctgtact gcggtatatt
     2761 ccccaaagca attctgaaat acaccagggc gaatgggtca agcgtacagg aagcgaactg
     2821 cgaggcaaga cgctggggct ggttggcttc ggcatgattg cccgccgcct ggcagaattg
     2881 ctcagggcat tcaacatgcg gataatcgcc tgtgatccat ggcctgaccg tggcgaggct
     2941 gaacggctgg gcgtggagct tgttacgctg cagcagcttt tgcaggagtc ggatattatc
     3001 agcctccatg cgccttatac tgctgaaaca cgcggtatga tcggtgccaa tgaactggca
     3061 ggtatgcggg acggtgcagt gctgatcaat tgtgctcgtg ccgggctggt agacacacct
     3121 gcgctggctg aagcgctgcg ttccggaaag cttgccgctg cagcattgga tgtattcgac
     3181 gaggagcctg tcagttcgtc caatcctctg attcatatgc caaatgtgat ttgtactgct
     3241 cactatggcg gaagtacttt cgaaagtatg tatatagaca gcatgatttc cgcacaagcc
     3301 attataagcg tactccgcgg tgaacagccg gaaaaccttc ttaacaggtg atacacctga
     3361 gaatattcag actatgcggc ggagacccgg gtgatcggtt tccgccctta ttttttcata
     3421 tcaaaatgcc ccggcttaaa ggtgaacaat atgtatattg tttcagacag atgtaaggac
     3481 atttctgaga aggtcaaaaa atataaaaat gagattttga tttttcttgc tatttctgtt
     3541 gctttgcttg gggatgccag cctgtatgta cttctgcctg tccatgcttc tgatcgtggg
     3601 atctcaattg cgtctttggg agttctgtta agtgtaaatc gccttgtcag gctggcagtg
     3661 aatagtttca taaaatatct gcataaatgg accggaagca ggacgctgtt tacggcaagc
     3721 ataacactaa cagcattatc aacattgatc tatggtttgc cggtaagttt cattaccctc
     3781 ctggcggcca gaattatttg gggatgctgc ttctcatcaa tgaggtatat tgcatatctg
     3841 aaaattgctg aaagttcaga tgacagcaac cggggagaac tgacaggatt gtataattct
     3901 gcgatcaggg tcggcagtct tattgctgcg cctttaggag cggctttggc tggcctcctg
     3961 gtatcgatac ggcactttta cactgtattc ctgtctgtat acttcgggag gctgtcggat
     4021 cgttacgggc gtttcagggt agtagtattg tcctgctgta tacaggcggt atccctggcg
     4081 gttattgctt taagccgatc gtttttgctg cttagcatgg cagtactggc agcattcaca
     4141 ggagctactg cattgacaaa tactctaagt gcggcagcaa tggactgtca ccatatttac
     4201 aaaaccaaaa acgtgcttgg atgctttact acctggtttg atataggatc tgcacttgga
     4261 actatggcag gttatacgct tatggtagcg attggataca gcagaacata tatatacagc
     4321 ggcctgattt taactgcagt cttagggctt attgcaagga agatgtttgc cggcgcagag
     4381 caggcaaatc aaaactgaca tcataattta ttgccgtatt tcttttcata tttacatgta
     4441 ttagaattat gctaaagaag gtatgcagat ccggcatatt tcacactgct atatcactat
     4501 cgactggaga tgatggaatg ggcagctttt tcaggaccga aggatcacgt cttatcaggg
     4561 aataccgcaa tgagatactc tggatcgagc catggggcgc caacagcctg cgcgtccgcg
     4621 ggacaaaacg cgcagcgatg gaacagcagc aggactgggc tttgctgccc ggacccggcg
     4681 gcgatgcggc ggagatctcc atatcggatg acggcaggtc ggcggagatc aggaacggca
     4741 agataactgc aaaaattttc cctaacggga aactagccta ttataattct gaaggcaggc
     4801 tgctgcttga agagtacgta aggagccgcg ataatctgaa ggagttcgtc agcgcgcttt
     4861 gcataaattc ccgggaatac tcccctgtcc ccgggaccaa tgatttcagg cttacggtaa
     4921 gattcgaggc caatgacgat gagaaactgt tcggaatggg acagtaccag cagccgtatc
     4981 tcgatctcaa gggctgcaca ttggaactgg cccacaggaa ctcccaggca agcgtaccgt
     5041 tcctgctttc gaacagggga tacggtttcc tctggaacaa ccccgctgtc ggcaaagtta
     5101 cgttcggcag gaacgtgacc gagtggtatg caggttccgt gcagcagatg gattactgga
     5161 taacggcagg tgatacgcct gcggagatag aggaagcgta cgcccgggtg acaggtactg
     5221 tgccgatgat gcccgattat gcgatgggtt tctggcagtg caagctgcgt taccagacgc
     5281 aggaggaact gctggaagtg gctcgtgagt acaagcgcag aggtctgccc atatctgtta
     5341 tcgttatcga cttcttccac tggacgaagc agggcgattt caaattcgac cccgaatact
     5401 ggcccgaccc ggaaggcatg gtgcgtgaac tcagggaaat ggggatcgaa ctcatggttt
     5461 ccatatggcc tacgatcgac aagaccagcg agaacttccg ggagatgcat gaaaagggat
     5521 accttgtcag gttcgacagg ggcaattcca tcgtgatgga ctggatgggc aacactatgt
     5581 tttttgatgc cacccatccc ggggcaaggg aacacctgtg gaaaatcata aaaaggaatt
     5641 attatgacaa gggcatcagg atattctggc tcgatgaggc cgaacccgag tattccgaat
     5701 acgaattcga caattaccgg tatcatatag gcaccgcgct gcaggtcagc aacatctatc
     5761 cgctgatgta cgcaaaaact ttttatgacg gcatgaaagc ggcgggccag gagaacataa
     5821 tcaacctcat ccgctgcgcc tgggcgggaa gccagcggta tggagcgctg gtatggtcgg
     5881 gggacatcca ttccagtttc agggctctcc gcaaccaggt gagggccggg ctcaacatgg
     5941 ccattgccgg tataccgtgg tggaccaccg atattggcgg tttccacagc ggagatatcc
     6001 gcgatcctgc tttccacgaa ctcattatcc gctggttcca gtatggtaca ttctgtccgg
     6061 tattccgact ccacggcgac agggaaccgc acacgccgcc catgggcacc tccggaggcg
     6121 gaatattcgg gagcggcgcc ccgaactttt tttttttcaa gcagaagacg gcatacgaga
     6181 tattcaaaaa
//
LOCUS       DAOSCC010000145         6188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8069_length_6188_cov_4.732920, whole genome shotgun sequence.
ACCESSION   DAOSCC010000145 DAOSCC010000000
VERSION     DAOSCC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8069_length_6188_cov_4.732920"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1268
                     /locus_tag="PLG72_08180"
     CDS             372..1268
                     /locus_tag="PLG72_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ80811.1"
                     /translation="MHMSIGDRIINFITYVVYSFFAFVCVYPFYYIFINSISANNLSD
                     KGKVLFWPLQIHFTNYVNVMKISTLFSALKVSVARTVIGTVLTVLFSAFLGYMFTRET
                     LWGRRFWYRFVVVTMYFNAGIIPWFITMMNLKLTNNFWGYIWPVVVQPFYVVLCKTFV
                     ESTPKELQEAAEIDGAGTLTIFFKVMLPVIKPILATIAIFAAVAQWNSFQDTLLLMTD
                     SKLYTLQFVLYQYINQANSLKGLVNNATGNIDIAASLAKAQTATSIRMTVTIIVVTPI
                     ILVYPIFQRFFVKGIMIGAVKG"
     gene            1437..>3008
                     /locus_tag="PLG72_08185"
     CDS             1437..>3008
                     /locus_tag="PLG72_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ80812.1"
                     /translation="MKRLLSIMLVVCLILTMALTGCGDTTSKGTGSDTSSGSGASTGG
                     DSTGSSTGDASAGGTREISHDKEITIDNYNVAANYHGEQTGWFGKLLKDKFNIKLNIM
                     APQVAGDALYQTRISSGNLGDIVILEGPDFADCVQNGLIKDISDEIKNWPNLMEYFNQ
                     IETYNKSIPGNEAGKIYGIPCQMTNTSPTAYSQDVIYSSPQLRWDLYQAIGAPVIQDL
                     EGLLDALEAIQKIHPTNEDGEPAYAMTLWPDWDGGDNMMGIANVVQLTTWYGEKIKGS
                     VVLKPDGTFYKLTDKNGTYRKMLHFLFEAQQRGLVDPDSMDQDWNAVYPKIASGRVHL
                     LWYSWQQGFWNTADRLAQGNAFIFIPVMDQNYYADSDTYYGSGRVFGVGSGVDDEKFL
                     RIMEFLDWYASPDGLEYQHIGIEGFNYTRGTDGKLTIMNENALMDNLPVPEEWGGGGF
                     NDGNNAINQWIVDSISINPKTGEPYSRTYWSSYKEATRTEMKRQWAEKFGAEEPAEWM
                     KKNNKLIVSPNVSVTL"
     assembly_gap    3009..3018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3019..3240
                     /locus_tag="PLG72_08190"
     CDS             <3019..3240
                     /locus_tag="PLG72_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80813.1"
                     /translation="NPNDIAVTRNSCEEILEEYSWKMIFCGSEEKFNQLWDEMVAKMD
                     GNDFDQVVQFDLEKWQIELDAKKAAMENQ"
     gene            3485..5017
                     /locus_tag="PLG72_08195"
     CDS             3485..5017
                     /locus_tag="PLG72_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOJ80814.1"
                     /translation="MLRIVKTENGIVQGLPAADPRITSFKGIPFAAPPVGENRWRAPQ
                     PAKNWDGVLKAFQFAPVSIQPQMGVDPNDIYVREWHVDPDTPMDEDCLYLNVWTPAKR
                     PDEKLPVFVWFFGGGLQCGYTSEMEFDGERIARRGIVVVTVNYRLNVFGFLCHPEITA
                     EAPEAPANFGHLDQQAGLKWVKRNIAAFGGDPDNITIGGQSAGGGSVLCQLTSPQNEG
                     LFNRAIIQSGMIAPVYPGNRVPRHGRTLSDAEKEGAEFFEFLGVKTLAEARKLDAKYI
                     LEKALQYKNFWSMLWSTVQDDKFCVGNPYELFIKNKRWMVPLLTGHTSSEFFSVPQVE
                     TMDEFRKMAVEMFAEDADEFLALCNAQSGNIEEVRKKASVSGLEYSIRIAAQANDRSK
                     TGVPIYYYNFDAEIPGWDNPGTFHSVDLWFTFETLAKCWRPFTGKHYDLARQMCNYWC
                     NFIKSGDPNGADSTGEEMERWEPYTTDAPYCMVFGDKPKFVREQPSGLMEFLVRQYFK
                     RK"
     gene            5288..>6188
                     /locus_tag="PLG72_08200"
     CDS             5288..>6188
                     /locus_tag="PLG72_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ80815.1"
                     /translation="MFEFWEVPQKYGREIHVNTNHPHASDVNPGTPSKPLKTINAAAR
                     IATPGTRVLIHGGTYRETVQPAIGGLSPEKMISYEACEGEEVIIKASVEVKEFRPSVG
                     WRLARGFGGKRVNTEGIRVWEIDMDPEDFKGYNPFCAVNILHDRLFIEYDKTDMTPYL
                     NRRGMVFVDGKPMKQVPLYYMLSENENAYWVEANGQKVHIRLANDDDPGNHTIELTNR
                     EQCFAPKVPFLSYIKVKGLTLAHAATGAPVPQRGSLSCYRGHHWIIEDCTIDWSNATG
                     IDCGNECWHHTVEEGQIIGHSIIR"
BASE COUNT         1719 a         1471 c         1662 g         1326 t
ORIGIN      
        1 gttctgttgt gtgctacaaa cggggtatcg aaacttgtga gaggcgaggg gttcatctga
       61 tcccgggatc catccggcct gcctggaagg aagggccgcc gtatgatcgg gccagatctt
      121 acaggaagga agcaacggct gccggctgac tcgttcttat agatggatac atagaacaac
      181 gattcccggt tagaaagaat ccaatacggt ttatatggcg gcttgcccaa aggacggttg
      241 ccggtccatc ggttttgatc catcggaaat ggaaagcaag gcggtcggtc catgaaaaat
      301 gctgtggccg gtacattgag atgggaggat ttgagatgag tgtttcaagg cagaaaactc
      361 aaaaacacaa aatgcatatg agtataggcg acagaatcat aaacttcatt acctatgtgg
      421 tatattcgtt ttttgcattc gtatgtgttt atccttttta ctacattttc atcaattcca
      481 tcagtgccaa caatctcagt gataaaggaa aggtactgtt ctggccgttg cagatacact
      541 tcaccaacta tgtcaacgtt atgaagatct ccaccctgtt cagcgccctt aaagtatcag
      601 tcgcaaggac tgttatcggt acggttctga ccgtcctgtt ctctgctttc ctcggataca
      661 tgttcacaag ggaaacactt tggggcagga gattctggta caggttcgtg gtggtcacca
      721 tgtactttaa cgcagggatc atcccgtggt ttatcacaat gatgaacctg aaactgacca
      781 acaacttctg gggatacata tggccggtag tcgtacagcc cttctacgta gtactgtgta
      841 agacttttgt cgaatccact cccaaggaat tgcaggaggc ggctgaaatc gacggggcag
      901 gtacactgac tatcttcttc aaagtgatgc tgccggtcat caagccgatc cttgcgacca
      961 tcgccatttt tgcagcagta gcacagtgga actcattcca ggatacgctg cttctgatga
     1021 cggacagtaa gctctatacg ctgcagttcg tgctgtacca gtatatcaat caggctaact
     1081 cgctcaaagg tctggtcaac aatgctacag gcaacataga tattgccgcg agccttgcaa
     1141 aagcccagac agctacatcc atacgaatga cagtaacgat catcgtcgta acgccaatta
     1201 tcctcgtata tcccattttc cagaggttct tcgtaaaagg catcatgata ggtgccgtaa
     1261 agggataact ttaagcactg cagggataga cactgtgaca gatcgtgaac agaagcaatg
     1321 gcatggcgta ctacggcaat aatgagtcag gcatcagcaa tacgacaaca gatatcaaca
     1381 atgaagccag tcttcattgt tcgattatag accaaaacaa agaggaggtt ccaactatga
     1441 aaaggttact gtcaatcatg cttgtggtct gccttatcct gaccatggcc ctgacgggat
     1501 gcggagatac cacaagcaaa ggtaccgggt cggatacatc gtccggcagc ggggcctcaa
     1561 caggcggaga tagtacagga agcagtacag gagatgcttc agcaggaggg accagagaga
     1621 tttcccatga taaggagatc accatagaca actacaatgt ggcggccaac tatcatggag
     1681 aacagaccgg ctggttcgga aaactcctga aggacaaatt caacatcaag ctcaacatca
     1741 tggcgcccca ggttgccggc gatgcactct accagacccg tatcagttcc ggtaatctcg
     1801 gagatatcgt aatacttgag ggcccggact ttgcagactg cgtacagaac ggacttatta
     1861 aggatatctc tgacgagatc aagaactggc ccaatctcat ggagtatttc aatcagatcg
     1921 agacctacaa caagagcatt cccggaaatg aagcagggaa gatatacggc atcccgtgcc
     1981 agatgaccaa tacgtccccg acagcttatt cacaggatgt catctactca agcccgcagc
     2041 tccgctggga tctgtaccag gcaatcggag ctcctgtaat acaggatctt gaagggctcc
     2101 tggatgcact tgaagcaata cagaagatcc atccgacaaa cgaagacggc gaaccggcat
     2161 atgcgatgac cctctggcct gactgggacg gcggcgacaa catgatggga atcgccaacg
     2221 ttgtccagct taccacatgg tacggcgaga aaataaaggg ttctgtcgtg ctgaagccgg
     2281 acggaacatt ctacaagttg acagacaaga acggaacata ccgcaagatg ctgcatttcc
     2341 tctttgaagc tcagcaaaga ggccttgtag atccggattc gatggatcag gactggaacg
     2401 cagtatatcc gaagatagct tcaggccgtg tacatctgct ctggtacagc tggcagcagg
     2461 gattctggaa cacggcggac aggcttgccc agggcaatgc gttcatcttc atacctgtaa
     2521 tggatcagaa ctattatgcg gattctgata catactatgg cagcggccgt gtattcggcg
     2581 taggttcggg agttgacgac gagaaattcc tcaggatcat ggaattcctc gactggtatg
     2641 caagtccgga cggcctggag tatcagcata taggtataga aggcttcaac tacacgagag
     2701 gaaccgacgg caagctgact ataatgaatg agaacgctct tatggataac ctgccggttc
     2761 ctgaagagtg gggcggcggc ggattcaacg acggcaacaa tgccatcaac cagtggatcg
     2821 ttgattccat aagcataaac ccgaagaccg gcgagccgta ttcaagaaca tactggagtt
     2881 catataagga agccaccagg actgagatga agaggcagtg ggctgaaaaa ttcggcgctg
     2941 aagaacctgc tgaatggatg aagaagaaca ataaactgat cgtcagcccg aacgtcagcg
     3001 taacgcttnn nnnnnnnnaa tccgaacgat attgcagtta cccgtaactc ctgcgaagag
     3061 atccttgagg aatactcatg gaagatgatc ttctgcggca gcgaagagaa gttcaaccag
     3121 ctgtgggatg agatggtagc gaagatggac ggaaacgact tcgaccaggt agtacagttt
     3181 gaccttgaaa agtggcagat agaactcgat gcaaagaaag cagcaatgga aaaccagtaa
     3241 tgctgatata gagtgagcag tgctgcgcat gggataagca gctaaataca ggcgtgaggg
     3301 gatcggtccc gcggatcggt tccctcatgt gttattgcct ggtgcaataa cggtatttcc
     3361 gccattttgt ggcttgccgg gcaatacagg aagtgctata aaggaattag aagcgttaaa
     3421 gtaaacagcc aagttaaaag agaagcaaaa tgatgatatg atataaagat atggagtgat
     3481 aatcatgctg agaattgtaa agacggaaaa tggtatcgtc cagggacttc ctgcggcaga
     3541 tccgcgtatc acgagtttca aaggcatccc gttcgctgct ccgcctgtcg gggaaaacag
     3601 atggcgcgct ccacagcctg cgaaaaactg ggatggtgta cttaaggcat tccagttcgc
     3661 gcctgtatca atacagcccc aaatgggagt tgacccgaac gacatatatg tcagggagtg
     3721 gcatgtggac ccggacaccc cgatggatga ggactgcctg tacctcaatg tgtggacgcc
     3781 ggcaaaaagg cctgacgaga agctgccggt gttcgtttgg ttcttcggcg gcggactcca
     3841 gtgcggatac acgtctgaaa tggagtttga cggtgagcgc atcgcgcgaa ggggcatcgt
     3901 cgtcgtcacg gtaaattacc gcctgaatgt cttcggattc ctgtgccatc ctgaaataac
     3961 cgcagaagcg cctgaagcgc ctgcgaattt cggccatctc gaccaacagg caggcctgaa
     4021 gtgggtcaaa cgcaacatcg cggcatttgg cggagacccg gacaacatca ccataggcgg
     4081 ccagtcggcc ggcggcggga gcgtgctgtg ccagctcact tcaccacaga acgaagggct
     4141 ctttaaccgc gctatcatac agagcggtat gatagcaccc gtctatcccg gaaaccgtgt
     4201 gccgaggcac gggcggacgc tgtctgacgc tgaaaaggaa ggcgccgagt tctttgagtt
     4261 cctgggcgtc aagacgctgg ccgaagccag gaaactcgac gcgaagtata tactcgaaaa
     4321 agcgctccag tacaagaact tctggagcat gctctggagc acggtccagg atgataaatt
     4381 ctgcgtgggc aatccttatg agcttttcat aaagaataaa cggtggatgg tgcccctgct
     4441 gacagggcat acttcatccg aattcttcag cgtcccgcag gtcgaaacga tggatgagtt
     4501 caggaaaatg gctgttgaaa tgttcgccga agatgccgat gagttcctcg cgctttgcaa
     4561 tgcgcagtcg ggaaatatcg aggaagtgcg caaaaaggcg tccgtgagcg gactcgaata
     4621 ttcgatccgc attgccgcgc aggcgaacga ccggagcaag accggcgtcc cgatatacta
     4681 ttacaacttc gacgccgaaa taccaggctg ggataatccg gggactttcc attccgtaga
     4741 tctctggttc acgttcgaaa cactggcgaa atgctggaga cccttcacag gcaagcatta
     4801 cgatctggca cgccagatgt gcaactattg gtgcaacttc ataaaatcag gcgaccccaa
     4861 cggagcggat tcgaccggtg aagagatgga gcgctgggaa ccttacacaa ccgatgcacc
     4921 atactgtatg gtcttcggag acaaaccgaa gttcgtcagg gaacagccca gcggcctgat
     4981 ggaattcctc gtaaggcagt attttaagcg taaatagagg ggacagttct caccttttca
     5041 gcaaaaacta aggaagggac agttctcact ttttagagaa gcagaggagt actgttcccg
     5101 cctccttagc agaaattgga ggggacagtt ctcacctttt tgacagatat gacaggggca
     5161 gaactttctt tacattcgca ggagatgcgc atataagtac agttgaaata agccaacgag
     5221 tattatttcg aaaggaaaat acaccatgac aggatatgat ccgaccgcag tcttgccgga
     5281 cggccgaatg tttgagttct gggaagtacc gcagaagtac ggccgtgaga tccatgtgaa
     5341 taccaaccat ccccatgcgt ctgacgtcaa tcccggcacg ccgtcaaaac cgctgaaaac
     5401 gatcaatgca gcagcgcgta ttgcaactcc ggggaccagg gtacttattc acggcggaac
     5461 ataccgcgaa acggtccagc ctgctattgg cggtctgagc cctgaaaaga tgatcagtta
     5521 cgaagcctgc gaaggggaag aggtgatcat caaagcttcg gtggaagtaa aggaattcag
     5581 gccgagtgta ggctggaggc tggccagagg ctttggcggc aaacgtgtga ataccgaagg
     5641 catcagggtg tgggagatag acatggatcc agaggacttc aagggctaca acccgttctg
     5701 cgcagttaac atccttcatg acaggctctt catcgagtat gacaagaccg atatgacgcc
     5761 ttacctgaac aggagaggga tggtgtttgt cgacggcaaa ccgatgaagc aggtcccgct
     5821 ctattacatg ctcagtgaaa acgagaatgc ttactgggtc gaagccaacg ggcaaaaagt
     5881 gcacatcagg ctagccaatg acgatgatcc cgggaaccat accatcgagt taaccaacag
     5941 ggagcagtgc tttgcgccca aagtcccatt cctgtcctat atcaaggtaa aagggctgac
     6001 gctcgcccat gcggcaacag gcgcacctgt accgcagcgc ggttcgctgt cctgttacag
     6061 aggccatcac tggatcatcg aggactgcac gatcgactgg tccaatgcaa ccggcataga
     6121 ctgtgggaac gagtgctggc atcacacagt tgaggaaggc cagatcatag gccactccat
     6181 catcaggc
//
LOCUS       DAOSCC010000146         6089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8224_length_6089_cov_3.435366, whole genome shotgun sequence.
ACCESSION   DAOSCC010000146 DAOSCC010000000
VERSION     DAOSCC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8224_length_6089_cov_3.435366"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLG72_08205"
     CDS             complement(<1..1023)
                     /locus_tag="PLG72_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ80816.1"
                     /translation="MMDSAHKGMFNRKPAGKWDEAYPSGNGVQGVLVMGEPLDETIIF
                     NHERLFLPTPVNAATVLPDMSGELPAVRRLIRDGKYREATRHFLNKAREKGFPSELIW
                     TDPFHPAFELKIKTVDAAAAPDYIRWLDFETGEAGVKWYGESGRYERRVFVSRACDAV
                     VIKLDAPSGGKLNCDIYLAERPGKAHIKWVSVKAGVFTDFVPHKPGRQDSDEQMPKQL
                     RSVKQPCPGEQMQNRNDIWLVFDSRYEAGSGGYCGIVRIHAEGGSAGLAGDTARLRNA
                     DGAVITARTLPYKDHSKFNLADAIEELAAIPMDYGMLLKEHADIHGGLFNRMRLELCR
                     ENNGSGT"
     gene            complement(1213..5502)
                     /locus_tag="PLG72_08210"
     CDS             complement(1213..5502)
                     /locus_tag="PLG72_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ80817.1"
                     /translation="MANNWTKEQLEAITERGCNLLVAAAAGAGKTAVLVERIIRKITD
                     PENPVDIDRLLVVTFTNAAATEMRERIGDALAEALEKDPSSVNLQKQMALLDRASIMT
                     LHSFCLEVVRSHFHALDLDPLFRIADETESALMKLEALDRLFESKYENEDAGSLFFKL
                     VESYGGGRDDSLLRDTVMTLHRFTSSHPYPEKWLAEQTEAYNAGANACMCGTPWARVL
                     LSSAAAELQGLMGMTGRAKERASRAEGLEPYCGTLDDDMKVLEKIRALCLDAMIKADG
                     ACGKADEGAARDADLAWDRLHDALASCELTRLPRCGKDADKTAQEEVKAVRNTVKERL
                     KKLQASGFDASSAEIREDFGKLYPQLKYLSGMVSEFDGIYRQMKKEKGVLDFNDLEHY
                     CLQILLEDGVPTAAARELRKRYEEILIDEYQDSNLIQELIISVVSGNDDDIYNIFMVG
                     DVKQSIYRFRQARPDLFLSKYQGYHRDKGYEDRVIQLYKNFRSRCEVINAVNLIFRRI
                     MSPEVGELDYTEQEYLNPGAVFPVPGHDCIAGGQVELHILDLLSETDEIKPDWNVKPE
                     GQADGETGSEAAGGDKPGNGAAASGISGSGAPGGDAGNGIGTGRNNANGNKDVHGMND
                     GDSKNGGMPENSGSGEDDEEPLDSIQYEARLVGSIIRRIVTHEPGGFSVFDKKVNAYR
                     PVEYRDIVILMRTTMKWADVFVDELALMGIPAYADTGLGYFRTVEVETMLSLLQIIDN
                     PMQDIPMLAVLRSPIGGFSTDDLADIRLADRSVSIYEAMRKLAGSYDTGKGDPSGSCR
                     TAGLDADKNDLADKSDGTDKSDVIKKTHDFLARLDRWRDAAQYTPTDELVWQLLSETG
                     YYSYVGALPGGAERKANLRMLFERARQYEETSYRGLFNFINFINRLRSGGGDMGSAKI
                     LGENDNVVRIMSIHKSKGLEFPVVIVAGCGKKFNMQDLNASILLHQDLGFGPDLVDLE
                     KRTIIPSLPKFAIRQKLRLETLSEEMRILYVAFTRAREKLIITGSVRDHAGKLASWRE
                     KAAGAADKLSPYDMMQASSYLDWIGPAVIKQDSPGATGDAHGTGDEDDIHLDEPLVEI
                     RFWGTGAARLEKGAPEAAGNIREWLREIHERDAESPQMPDEGTKRPEQEERPESVGHA
                     DVQQIIEALEWTYPYQKLTTVPAKISVTELKRRALQEEVTESAVPYIQPMQERPLFME
                     PDKGLSAAHRGTVMHFVMQHLDLGRLYEAAKSKAKDAEPSSAPSGTADSTGTAEGNVL
                     PMALRPGTDADLTDGLVPEISSQISSMKDMELITPAEAESVDPEAVAAFFRSPFGMRM
                     LSLHDSIHRETAFTIEIKCSEVLKDDAEGAACDETMLLQGVIDCWFDTGDGLVLLDYK
                     TDYVPGGRSDIIRDRYKVQIEYYTKALEKITGKKVTERYLYLFRSGELVAC"
     gene            complement(5663..6089)
                     /locus_tag="PLG72_08215"
                     /pseudo
     CDS             complement(5663..6089)
                     /locus_tag="PLG72_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514090.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         1140 a         1844 c         1454 g         1651 t
ORIGIN      
        1 tgtacctgat ccattatttt cgcggcacag ttcgagacgc atgcggttga acaacccgcc
       61 atgtatatct gcatgctctt tcaaaagcat gccgtaatcc atcggaatgg cagccagttc
      121 ctcaatcgca tccgcaaggt tgaatttgga gtgatccttg tatgggagcg tccgtgcagt
      181 tatgaccgcg ccatcggcat tgcggagcct tgccgtgtcg cctgcaaggc cggcagatcc
      241 gccttccgca tgtattctga ctattccgca gtaaccgccg ctgcctgctt catatctgct
      301 gtcgaatacg agccatatgt cattcctgtt ttgcatctgt tctccgggac acggctgttt
      361 gacggaacgg agctgtttcg gcatttgctc gtcagaatcc tgccgtcccg gcttatgcgg
      421 aacgaaatca gtgaatacgc ctgcttttac agatacccat tttatatgtg ctttcccagg
      481 gcgttctgcc agataaatat cacagttcag tttgcctccg gacggcgcgt caagttttat
      541 aaccaccgca tcacatgccc tggaaacaaa aacacgcctt tcgtaccgtc cgctttcacc
      601 ataccatttg acgccggcct ctcccgtctc gaaatccagc cagcggatat aatcaggcgc
      661 tgccgctgcg tctactgttt ttattttcag ttcgaatgcc ggatggaaag gatcggtcca
      721 gatcagttcg gaaggaaaac ccttttccct tgccttgtta aggaaatgcc tcgtggcttc
      781 cctgtatttc ccatccctga tcagccttcg cacagccgga agttcgcccg acatgtcggg
      841 aaggacagtg gccgcattga caggtgtcgg aaggaacagc ctttcatggt tgaatataat
      901 ggtctcgtca agcggttcgc ccatcacaag gactccctgt acgccgttcc ctgacggata
      961 ggcttcgtcc cacttgcctg ccggtttcct gttaaacatg cccttatgag cagaatccat
     1021 catgatgcac ctcttgcctg atttttatct gttttcaggt atcttttgcc atgcctgatt
     1081 tagtaagttt accatatttt aattaccgtt actgcaatat tagacggaaa ctgcaaaatc
     1141 cgcccggagc acagaagcac cgactcatgg ccttcaccgg caaacatttg atacgaaact
     1201 gcccgtacgg catcagcatg ctacaagttc gccgctgcgg aacagataca ggtacctttc
     1261 ggtcaccttt ttgccggtga tcttctccag cgcctttgtg taatattcta tctggacttt
     1321 atacctgtcc ctgattatat cggaacgccc tccaggcaca tagtcggtct tgtaatccag
     1381 cagcacgagc ccgtctccgg tatcgaacca gcagtcgatg acgccctgca gcagcatcgt
     1441 ttcgtcgcag gccgcgcctt cagcatcgtc tttgagcacc tccgaacact ttatctcgat
     1501 ggtgaaggca gtttccctgt gtatggaatc atgcagggaa agcatgcgca tcccaaacgg
     1561 agacctgaag aatgcagcaa ctgcctccgg atcgacagac tccgcttccg caggcgtgat
     1621 cagttccatg tctttcatgg acgatatctg ggaacttatc tccgggacca gtccgtcagt
     1681 cagatccgcg tctgtcccgg gtcgcaatgc cattggcagc acatttcctt ccgcagttcc
     1741 tgttgagtct gcagttccgg atggtgcgga agacggttcg gcgtcttttg cttttgattt
     1801 tgctgcttca tacagcctgc ccaggtccag gtgctgcatg acgaaatgca tcaccgtgcc
     1861 cctgtgggcg gcgctcaggc ctttatcagg ttccatgaac aaaggccttt cctgcatggg
     1921 ctggatatac ggaacagcag actcggtaac ttcctcctgc aatgccctgc gcttgagttc
     1981 agtcaccgat atctttgcag gcacggtcgt cagcttctga tatggatacg tccattcaag
     2041 agcctcgatg atctgctgta catcagcatg tccgacgctt tccggacgct cttcctgctc
     2101 cgggcgtttg gttccttcat caggcatttg cggactttcc gcgtcccgct catgtatttc
     2161 acgcagccat tccctgatgt ttccggcagc ttcaggcgct cccttttcga gccgggcagc
     2221 ccctgttccc cagaatctta tctccaccaa aggttcatcc aggtgtatat catcttcatc
     2281 gccggtcccg tgtgcatcgc ctgttgctcc gggagagtcc tgttttatga cggcgggacc
     2341 tatccagtcc aggtatgaag atgcctgcat catgtcatat ggcgacagct tgtcagccgc
     2401 gcctgccgct ttttcgcgcc atgaagccag cttcccggcg tgatccctga cgcttccggt
     2461 tattatcagt ttctcccttg cccttgtaaa ggctacgtaa agtatgcgca tttcttccga
     2521 cagcgtttcc agcctcagtt tctgtcttat ggcaaacttg ggcagggacg ggattatcgt
     2581 ccttttttcc aggtcaacaa gatccgggcc aaagccaaga tcctggtgca gaagtattga
     2641 tgcgttcagg tcctgcatat tgaacttttt gccgcatccg gcgacgatga cgacagggaa
     2701 ctccagtccc ttgctcttgt ggatgctcat gatcctcacc acgttgtcgt tctccccgag
     2761 gatctttgcg ctgcccatgt ccccgccgcc gcttcttagc ctgtttatga agtttatgaa
     2821 gttgaagagt cccctgtaac tggtttcttc gtattgcctg gcccgttcga acagcatcct
     2881 gaggttggcc ttccgctcgg ctccgcccgg cagtgcgccg acatagctgt aatacccggt
     2941 ttcggagaga agctgccata caagttcatc tgtaggcgta tactgtgccg cgtccctcca
     3001 tctgtccagt ctggccagaa aatcatgcgt tttctttatt acatcgctct tgtccgtgcc
     3061 atcgctttta tcagcgagat cgtttttatc ggcatccagg ccggccgtac gacatgagcc
     3121 ggatggatct cccttgccgg tatcatacga cccggccagt ttcctcatcg cttcgtaaat
     3181 ggataccgac ctgtcggcaa gccttatatc cgccaggtcg tccgtactga agccgcctat
     3241 cggcgaccgg aggacagcaa gcatcggaat gtcctgcata ggattatcga ttatctgcag
     3301 gagagacagc atcgtttcaa cctcgacagt cctgaagtat ccgagtcctg tgtcagcata
     3361 ggccggtatc cccatcagcg caagttcgtc gacaaaaaca tcggcccatt tcatggtcgt
     3421 cctcatgagt atgacgatat ccctgtactc gacaggcctg tatgcgttca cctttttgtc
     3481 gaatacgctg aaaccgccgg gctcatgtgt gacgatccgc ctgattatgc tgccgaccag
     3541 gcgcgcttca tactggatac tgtccagcgg ttcctcgtca tcttctccgc tcccgctgtt
     3601 ttccggcatc ccgccatttt tggaatcgcc gtcgttcatg ccgtgcacgt ctttattgcc
     3661 atttgcattg ttcctgcctg tgccgatgcc attgcctgca tcaccgccag gcgcaccgct
     3721 gccggatatt ccactggctg ccgcgccatt gccgggttta tcgccgccgg cagcttcact
     3781 gcctgtttct ccgtccgcct gcccttccgg ttttacgttc cagtccggct ttatctcatc
     3841 cgtttccgac aacaggtcaa gtatatgcag ttctacctgc cctccggcta tgcagtcatg
     3901 cccggggaca gggaagaccg ctcccggatt cagatattcc tgttccgtgt agtcaagctc
     3961 gccgacctca ggtgacatta tccgtctgaa aatgagattt accgcgttta tgacctcgca
     4021 cctgctccgg aagtttttat aaagctgtat gaccctgtct tcgtaccctt tatcgcgatg
     4081 atagccctgg tacttggaaa ggaacaggtc gggccttgcc tgcctgaacc gatatatgct
     4141 ctgttttaca tcgcctacca tgaatatgtt gtatatatcg tcgtcatttc cggataccac
     4201 cgatatgata agttcctgga taaggttgct gtcctggtac tcgtctataa gtatctcctc
     4261 gtagcgtttc ctgagttccc tggcagccgc cgttgggacg ccgtcctcga ggagtatctg
     4321 caggcagtaa tgctcaaggt cgttgaaatc gaggacccct ttttccttct tcatctgccg
     4381 gtatattccg tcgaactccg ataccatgcc ggacagatac ttaagctgcg gatacagttt
     4441 gccgaaatct tcccttattt cagcgctgga ggcgtcaaat ccgctggcct gcagtttttt
     4501 gagcctctcc ttcaccgtgt tccttacagc cttgacctct tcctgtgcgg tcttatcggc
     4561 gtctttcccg cacctcggga gccttgtgag ctcacatgag gctaatgcgt catgcagcct
     4621 gtcccacgca aggtccgcgt cgcgggccgc tccctcgtcg gctttcccgc aggctccatc
     4681 tgcctttatc atggcatcga gacacagggc tcggattttt tcgagtacct tcatatcatc
     4741 atcaagggtg ccgcagtacg gctcaaggcc ttccgcccgg cttgcccgtt cctttgctct
     4801 ccctgtcatg cccatcaggc cctgcagttc cgccgctgca ctgctcagca gcacacgggc
     4861 ccacggcgtg ccgcacatgc aggcatttgc tccggcgtta tatgcttccg tctgttcggc
     4921 aagccatttc tcggggtatg ggtggctgga cgtaaaccgg tgcagcgtca tgacagtatc
     4981 ccggagcaaa ctgtcatcac ggcctccgcc gtagctttca accagtttga agaaaaggct
     5041 gccggcgtct tcattctcgt atttgctttc gaaaagcctg tcaagcgcct cgagcttcat
     5101 cagcgcagac tcggtctcat cggcgatgcg aaaaagggga tccagatcca gggcatggaa
     5161 atggcttctt accacctcca ggcagaatga gtggagcgtc attatacttg cgcggtccag
     5221 cagcgccatc tgcttctgca ggttgacaga tgaaggatcc ttttccagcg cctcggccaa
     5281 tgcgtcgcct atcctctcgc gcatctcggt cgccgctgca ttggtgaacg taacgaccaa
     5341 cagcctgtct atatctaccg ggttttccgg atcagttatt ttccgtatta tcctctctac
     5401 gagcacagca gtcttgcctg cgcctgccgc tgccgctacc agcaggttgc accctctttc
     5461 ggtgatggct tcaagctgct cttttgtcca attatttgcc attctccgaa tccctccgat
     5521 cccttaccgg cacaggctgc ttgtcctgtc cggctgcctg ccgttctgcc gcatcctgtc
     5581 ccggtgaatc ctgtacagct gaccctgccg tatcctgcgc tgcgtgtccg gctgcatcat
     5641 ctgcgccatt attccgccca cctatcagcc tccatacctc gtcatcgtcc aggtcggcca
     5701 ggtaacggta ccggttgtcc cgcatcgccg tgtcgaactg gcatacagaa ctgtagacgc
     5761 agtactcgca ggcggtcatc ttctgccttt tatacgggtt tacggccacg ttgccgtcca
     5821 ggatgctttt gccgatggag ccgaggagct tcctcacatg cccgcacagt tccctgaact
     5881 gtttctccgt ggcggcggac gatctcccga gcgacccgtc cttatttatc cttgccggga
     5941 tgatcagcga gtcgccagca atatctttgt ccatttccct gatcagtttc acgtctgcaa
     6001 gcaccaggcc cttcatcctc aactctttca ttattgcctt ctctatctct tcttcggtgc
     6061 tgtccctccc gcacctgata agcgggtcg
//
LOCUS       DAOSCC010000147         6084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8231_length_6084_cov_5.410184, whole genome shotgun sequence.
ACCESSION   DAOSCC010000147 DAOSCC010000000
VERSION     DAOSCC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8231_length_6084_cov_5.410184"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLG72_08220"
     CDS             <1..228
                     /locus_tag="PLG72_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ80818.1"
                     /translation="VTYLGPLLAYTLTGSFVVEKIFTIPGLGNDFISSITNRDYMLIM
                     GTTIFLAALLITMNLVVDIAYKLIDPRIKLK"
     gene            318..1367
                     /locus_tag="PLG72_08225"
     CDS             318..1367
                     /locus_tag="PLG72_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80819.1"
                     /translation="MTKEKNPLSLQLDVSDFLPADNEEKQSLVVMRQSVSFWRDGLRR
                     FRKNKIAMTSLVIIILTMIFAFIGPVFYPYTYEQQIRGSENLAPMQYSETELALKAQG
                     EKVFPHILGTDSLGRDTMVRLMMGSRVSLLVGIVASVLVLLIGSIYGAVAGYFGGKVD
                     LIMMRIVDIIYTVPDILIIILLMVTLKYPLENLANTVPGFAWINTIGVGLICIFIVFS
                     LLYWVGMARIVRSQILMLKEQEFVTAAKALGASHRRIIRKHLLTNCMGTLIVTTTLQI
                     PSSIFTESFLSFIGIGVAAPMPSLGSMASQAINGISSYPYRLLAPAIMISLIILSFNL
                     IGDGLRDAFDPKLKD"
     gene            1421..2422
                     /locus_tag="PLG72_08230"
     CDS             1421..2422
                     /locus_tag="PLG72_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80820.1"
                     /translation="MGKTLVDIKNLRVSFFTPAGEVKAINDVSLKMEEGEVLGIVGES
                     GSGKSVTAYSLMGLIPYPGRIIGGTIDFNGHRINDMTEIEMREIRGNEVSIIFQDPMT
                     SLNPVYTIGNQIMEVILLHTDKDKKQAYDRAVELLTLVGINEPKKRMKQYPHELSGGM
                     RQRVMIATALACEPKLLIADEPTTALDVTIQAQILELMIELKQKIGMSIIMITHDLGI
                     VANMCQKIAVMYAGKIVEYGTADEIFYNPKHEYTKGLLRSIPRLTEKEHRKLIPIEGT
                     PVDMLNPPAGCPFAPRCSSCMKICLGRMPEYTHLSETHYSACWLLQKEEFERRGQEA"
     gene            2419..3387
                     /locus_tag="PLG72_08235"
     CDS             2419..3387
                     /locus_tag="PLG72_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80821.1"
                     /translation="MSTLVEVKNLKQYFRVGGSLFSPRYVKAVDDVSFHIDKGETLGL
                     VGESGCGKTTTGRTILRLYEPTGGQIIYDGADITKANMLPYRRKMQIVFQDPYASLDP
                     RMTVGDIVGEAIDIHKLASDKHERKEKIYAMLERVGLNSEHANRYPHEFSGGQRQRIG
                     IARALAVDPEFIVCDEPISALDVSIQAQIINMFEELQEDLGLTYLFIAHDLNVVRHIS
                     NRIGVMYLGKLVELADSDELTFHSLHPYTKSLISAIPIPDPVESRSKKRIVLEGDVPS
                     PLDPPSGCRFRTRCPYATEICAQQEPEFREVSKGHQVACHHIDKIN"
     gene            3528..5114
                     /locus_tag="PLG72_08240"
     CDS             3528..5114
                     /locus_tag="PLG72_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ80822.1"
                     /translation="MRRKTALLLSLVLAFSVVLAACSGTSKTDVLTVHVGSDPDTIDP
                     ALNSAVDGATLIIHAFEGLMTLDKNGVPVKAQAKDYKVSDDGTVYTFTLRDDIKWSDG
                     QPVKASDFVYAWNRAISPDTAADYEYMFEVIDGYDEGTLNVQAPDDKTLVVTLKSPVP
                     YFLELCAFPTYFPVRQDIVEKYSDTWAVNPETYIGNGPYKIAEWVPGSHILYEKNKNY
                     YNYKDLGPEKIKFVLMDDDNAIKSAFENEEILFADSWPNDEIDAWRDKPEFHIEGQLG
                     TYYVSFNTKKPPLDNPKVRKALTLAVDRDFIVRNIGKAGQQPAGAFVPTGLSDADPTK
                     EFREVGGDYYDPSKEAYAANLEEAKRLLAEAGYPNGEGFPTLTYLYNESTGHQQIGEA
                     LQDMWKQIGVNVKLESQEWNTFLNTRKNGDYDIARNGWLGDYNDPISFLDMWVTGSGN
                     NDAQWSNAQYDELIRKIKVETNTEERYKMMHQAEDIIFEESMLCPIYYYVDIYLLNQK
                     VEGFYSSPLGYKYFMYCSVK"
     gene            complement(5344..5979)
                     /locus_tag="PLG72_08245"
     CDS             complement(5344..5979)
                     /locus_tag="PLG72_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOJ80823.1"
                     /translation="MAANYSSLTAKQKKVYMVIESYIKTNGIPPTVREIGELVGEKTP
                     GAVQGILNRLEQKGVIKRQLGAARSIQLVSPDDQMYGKPVFVPKIKKISKRNINDLTN
                     IYNIELYHPFSQEVLSENGDYILSECPADGLTESGFGSNDMILVNRTAELKPGDIVLA
                     FYETISLLRRYYPESNGTITLKADSKVIDKENFTPDEIAIIGKVEGRYTKF"
BASE COUNT         1658 a         1434 c         1570 g         1422 t
ORIGIN      
        1 gtcacctacc ttggtccgtt gcttgcatac acactgacgg ggagcttcgt tgtcgagaaa
       61 atattcacga tacccgggct tggcaatgac ttcataagct ccatcacaaa ccgggattat
      121 atgctgatca tgggcactac gatctttctg gcggcattgc tgatcaccat gaacctggtg
      181 gtcgacattg cgtataagct gatagacccg aggatcaagc tgaaatagtc cgtgcggaac
      241 aggaagcggc tcagcgacgc cgcggatcgg ggtcatcgga tagtgaacaa aaaaggaacg
      301 gggagcgata caagaaaatg acgaaggaga agaatccact tagtttgcag ctggatgttt
      361 cggattttct gccggctgac aacgaggaga agcaaagcct tgtggtcatg cgccaaagcg
      421 tgagcttctg gcgtgacggg ctgcgccggt tcagaaagaa taaaatagcc atgacaagcc
      481 ttgtaattat aatattaaca atgatattcg catttatagg gcctgtcttc tacccctaca
      541 catacgagca gcagataagg gggagcgaaa acctggctcc gatgcaatat tcagaaacgg
      601 agcttgcact gaaggcacag ggagagaaag tgttcccgca tatactgggg accgactcgc
      661 ttggacgtga taccatggtc cgtcttatga tgggcagccg cgtatcgctg ctggtaggaa
      721 tcgtagccag tgtgctggtg ctcctgatag gctcgatcta cggtgctgtc gcaggatact
      781 tcggcggcaa ggtggatttg ataatgatgc gtattgtcga tatcatttat accgtgcctg
      841 acatactgat catcatactg ctgatggtca cactgaaata tcctttggag aacctggcga
      901 atactgtgcc cggatttgcc tggatcaata caataggtgt cgggttgata tgcatattta
      961 ttgttttctc gctcctctac tgggtgggga tggccaggat agtcaggagc cagatactga
     1021 tgctgaagga gcaggaattc gtaacagcgg caaaggcgct tggagcgagc cacagaagga
     1081 taataaggaa gcatctgctg accaactgta tgggtacgct gatcgttacg acgacactgc
     1141 agatcccgtc atcgattttt actgaaagct tcctcagctt cataggtata ggcgttgcag
     1201 ccccgatgcc gtcgctcggt tcgatggctt cacaggctat aaatgggata tcttcatatc
     1261 cgtacaggct tctggcgcca gcgatcatga taagcctgat aatactttcg ttcaacctga
     1321 tcggggacgg actgagagat gcattcgacc cgaagctgaa agactgatgc aaaattgcag
     1381 caaagcaggg ctgcggtatt gatagattga ggtgaaaaaa atgggcaaaa cactggttga
     1441 tataaagaat ctgcgtgtct ccttcttcac acccgcggga gaagtgaaag cgatcaacga
     1501 tgtttcgctg aagatggaag aaggagaagt actcgggatc gtcggagaaa gcggcagcgg
     1561 aaagagtgtt accgcatatt cgctgatggg gctgatcccg tatcccggaa ggatcatcgg
     1621 aggaactatc gattttaacg gacacaggat caatgacatg actgaaatcg aaatgcggga
     1681 gatcaggggc aatgaggtca gcatcatatt ccaggacccg atgaccagcc tgaaccctgt
     1741 gtacactata ggcaatcaga taatggaagt gatcctgctc cataccgaca aggacaaaaa
     1801 gcaggcgtac gaccgggctg ttgaactgct gacgctggtc ggcatcaatg aacctaaaaa
     1861 gaggatgaag caatatcctc acgaattgtc cggcggcatg cgccagaggg taatgatagc
     1921 caccgcgctg gcctgcgaac ccaagctgct tatcgcggac gagcctacga ctgccctgga
     1981 cgtgacaata caggcgcaga tactggaact gatgatagaa ctgaagcaaa agataggtat
     2041 gtccatcatc atgataaccc atgatttggg catcgtagcc aacatgtgcc agaaaatcgc
     2101 ggtcatgtat gccggaaaaa tagtagagta cggcacggcc gatgaaatat tctacaaccc
     2161 gaagcatgag tacacgaaag gactgctcag gagcatacca aggcttacgg aaaaggagca
     2221 ccgtaagctc atccccatcg aaggcacacc ggtggatatg ctcaacccgc ctgccgggtg
     2281 tccttttgca ccccgctgca gcagctgcat gaagatatgc cttggcagga tgccggaata
     2341 tacgcacctg tccgaaaccc attacagtgc atgctggctg ttgcagaagg aagaatttga
     2401 aaggaggggg caggaagcat gagcacactg gttgaagtaa agaatctgaa gcagtatttc
     2461 cgtgtgggag gaagcctctt tagccccagg tatgtcaagg ctgtcgatga tgtgagtttc
     2521 catatagaca agggagagac cctcggtctg gtcggtgaga gcggctgcgg gaagacgacc
     2581 acgggccgta ccatcctgag gctttatgag ccgactggcg gccagatcat ttatgacggc
     2641 gctgatatca caaaagcaaa catgctgccg taccggcgca agatgcagat agtcttccag
     2701 gatccgtacg caagcctcga tccgagaatg actgtcggag acatagtcgg agaggccata
     2761 gatatacaca aactggcatc ggacaaacac gagaggaagg aaaagatata tgcgatgctg
     2821 gagcgggtcg gcctgaacag cgaacatgcg aaccgctacc cgcacgaatt ttccggaggc
     2881 cagcgccagc gtatcggcat cgcgcgcgcc cttgcggtcg acccggaatt catcgtgtgc
     2941 gacgagccga tatcggccct ggacgtttcg atccaggcgc agataatcaa tatgtttgaa
     3001 gaactgcagg aggacctggg actgacatac ctgttcatcg cccatgacct caatgtggtc
     3061 aggcatataa gcaacaggat aggcgtcatg taccttggga agcttgtgga actcgcagac
     3121 agtgacgaac tgactttcca cagcctgcat ccgtatacaa aatctctgat ttcagccata
     3181 ccgatcccgg atcctgtgga aagccgcagc aaaaagagga tcgtactcga aggggatgtg
     3241 ccaagtccgc tggatcctcc gtcagggtgc aggttcagga ccaggtgtcc ctatgcgact
     3301 gagatctgcg cacagcagga gcctgagttc cgtgaagttt caaaaggaca tcaggttgca
     3361 tgccatcata ttgacaaaat caactgacag gataaaattt gtttaaatgc tgccattcag
     3421 ggggattata tcaaataaat catttgatgt atgactcctg gatgattgca tttcaatatt
     3481 acagtacata tatgtactga aataaaaatt tatgaggagg aagatttatg agaaggaaga
     3541 cagcattact tttgtcgctg gtccttgcat tcagcgtggt acttgctgca tgctcaggca
     3601 ccagtaagac agacgtactt acggttcatg tcggatcgga cccggatacc atcgacccgg
     3661 ccctgaactc ggccgttgat ggcgccacac tgatcatcca tgcattcgaa ggccttatga
     3721 cgctcgacaa gaacggagtg cctgtaaagg cgcaggctaa ggactacaag gtcagcgatg
     3781 acggaacagt ctatacgttc actctgcgcg acgacatcaa gtggagcgac ggccagcctg
     3841 tcaaggcgag cgatttcgtt tacgcgtgga accgtgcgat ctcgcctgac actgcagctg
     3901 actatgaata catgttcgaa gtcatcgatg gttatgatga aggaaccctg aatgtacagg
     3961 ctcccgacga caagactctc gttgtcacac tgaagtctcc tgtcccgtat ttcctggagc
     4021 tctgcgcatt cccgacctac ttccctgtac gccaggatat agttgagaag tatagcgata
     4081 cctgggcagt aaatcccgag acatatatag gaaacggacc ttacaagata gctgaatggg
     4141 tccccggttc ccacatcctc tatgaaaaga acaagaacta ctataattac aaagatcttg
     4201 gtcccgagaa gatcaagttc gtcctgatgg atgatgacaa tgcaataaag agcgcatttg
     4261 agaacgaaga aatactgttt gcagattcat ggcccaacga tgagatcgat gcctggagag
     4321 acaagcccga gttccatatc gagggccagc tcggaacata ctacgtatca ttcaacacaa
     4381 agaagcctcc gctggacaat ccgaaagtcc gtaaggctct gacgctcgca gtcgaccgtg
     4441 atttcatagt caggaatata ggcaaggccg gacagcagcc tgccggcgca ttcgttccga
     4501 caggactgtc tgatgcggat ccgaccaagg aattccgtga agtaggcggc gactactatg
     4561 atccttcaaa agaagcatat gcagctaacc tggaagaggc aaagagactg cttgctgaag
     4621 ctggttaccc gaacggagaa ggattcccga ctctgacata cctctataat gaatcgacag
     4681 gtcaccagca gataggcgaa gctctgcagg acatgtggaa gcagataggc gtcaacgtca
     4741 agctggaatc ccaggaatgg aacacattcc tcaatacacg taagaacggc gactatgaca
     4801 ttgcacgtaa tggctggctc ggtgactaca acgacccgat ctcgttcctc gacatgtggg
     4861 taacaggcag cggcaacaac gatgcgcagt ggagcaatgc tcagtatgac gagctcatca
     4921 ggaaaatcaa ggttgagacc aacactgaag agcgttataa gatgatgcac caggcagaag
     4981 acataatctt cgaggaaagc atgctctgcc cgatatacta ctatgtcgac atttatctgc
     5041 tcaaccagaa ggtcgaagga ttctactctt caccgctggg ctacaagtac ttcatgtact
     5101 gcagcgtgaa gtaagaacta tttcgcccgt atccggacga gatgaaaggc cggctcgaat
     5161 gagccggcct tttaagtttc tgatgcatgg tacaccagtt aagacatggg gacggtttcc
     5221 gtgtccggcg ggacatccgc cgtgcatgaa aaccgtcccc ttcctttgcc gttccctctt
     5281 ccgggaactt tccgtttctg ataaatcact tgtgcttttt caaaaatccc gggtattttg
     5341 atttcagaat ttcgtgtatc ttccttccac ttttccgatt atggcgatct cgtcaggtgt
     5401 gaaattctcc ttgtcgatca cttttgagtc ggctttgagc gtaatggtgc cgttgctttc
     5461 aggataatac cttctaagca gtgaaatcgt ttcataaaaa gcaagtacta tatcgccggg
     5521 tttcagttct gccgtcctgt tgacgagtat catatcgttt gacccgaaac cgctttctgt
     5581 cagcccgtca gccgggcatt cggaaaggat ataatcgccg ttttcagaca gtacttcctg
     5641 tgaaaacgga tgatacaact cgatattata gatattcgtc aggtcattaa tgtttctctt
     5701 gctgatcttc ttgatcttgg gcacgaaaac aggtttgccg tacatctgat catccggaga
     5761 aacaagctgg atcgaccttg cggctccaag ctgccttttt atgacacctt tctgttcaag
     5821 cctgttcagt attccctgaa cagccccggg ggttttttcg ccgaccagtt caccaatttc
     5881 ccgtactgtt ggaggtatac cgttggtctt gatataagac tcgatcacca tgtatacctt
     5941 tttctgttta gcagttagac tgctatagtt agctgccatc ttatcaccct ttgtatctgt
     6001 atacttttgt atttatatac tttacataat tcacaaaaca gtttagcaga ataattttaa
     6061 ataatcaaat taaaatctta cagt
//
LOCUS       DAOSCC010000148         6054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8285_length_6054_cov_4.758293, whole genome shotgun sequence.
ACCESSION   DAOSCC010000148 DAOSCC010000000
VERSION     DAOSCC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_6054_cov_4.758293"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            494..643
                     /gene="rpmG"
                     /locus_tag="PLG72_08250"
     CDS             494..643
                     /gene="rpmG"
                     /locus_tag="PLG72_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOJ80824.1"
                     /translation="MRVKIVMACTECKQRNYNTMKNKKNDPDRIEMKKYCKFCHKHTV
                     HKETK"
     gene            662..907
                     /gene="secE"
                     /locus_tag="PLG72_08255"
     CDS             662..907
                     /gene="secE"
                     /locus_tag="PLG72_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOJ80825.1"
                     /translation="MSENARTSKPAGFGSKLANLGNRIARFFRDIRNELRKVIWPTKE
                     QLVKSTISVLAICLIIGAVIWVCDAVFAKLMEWTLSR"
     gene            929..1462
                     /gene="nusG"
                     /locus_tag="PLG72_08260"
     CDS             929..1462
                     /gene="nusG"
                     /locus_tag="PLG72_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOJ80826.1"
                     /translation="MAVYDMPDEPKWYVVHTYSGYENKVKANLEKIVENRNLHDYIFD
                     IVVPMEEQIEIKDGRKKATLRKVFPGYVLVKMIMTDESWYVVRNTRGVTGFVGPGSKP
                     VPLSDEEVRNMGVEEFAPVLDYEVNDTVRVIDGPLENFIGTVEEINFEKRKVRVAVSM
                     FGRETPVELELTQIQKI"
     gene            1569..1994
                     /gene="rplK"
                     /locus_tag="PLG72_08265"
     CDS             1569..1994
                     /gene="rplK"
                     /locus_tag="PLG72_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ80827.1"
                     /translation="MAKKIIGYVKLQIPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTAKDAGLIIPVIITVYADRSFSFVTKTPPAAVLLKRACKIESGSGRPNRDKVAKISK
                     EEIRKIAELKMPDLNAASIEAAMSMVAGTARSMGIVVED"
     gene            2057..2752
                     /gene="rplA"
                     /locus_tag="PLG72_08270"
     CDS             2057..2752
                     /gene="rplA"
                     /locus_tag="PLG72_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ80828.1"
                     /translation="MKRGKRYIESAKLIDRAKLYDPVEALDLVKKTAKAKFDETVEVH
                     IKLGVDSRHADQQVRGAVVLPHGTGKTVRVLVFAKGDKAKEAEDNGADYVGAEDLVAK
                     IQNENWFDFDVVVATPDMMGVVGRLGKILGPKGLMPNPKAGTVTMDVAKAIAEIKAGK
                     IEYRLDKTNIIHCPVGKASFDLDKLTDNFRTLLGAIIKAKPAAAKGQYLRSVVVTSTM
                     GPGIKVNPQRIAE"
     misc_feature    2814..2986
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2998..3537
                     /gene="rplJ"
                     /locus_tag="PLG72_08275"
     CDS             2998..3537
                     /gene="rplJ"
                     /locus_tag="PLG72_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ80829.1"
                     /translation="MPKEYTLNQKREVIDRISSRMKAAKTMIFADYRGLTVEQDTELR
                     RALRNAGVEYKVVKNTLTKFAAKENGLEELYPYLNGPTSMASSDTDPVAPAKVLCDFA
                     KKYESFELKVGVVEGKVMDVDGIKALAELPSREVLIARVLGGFNAPISGFVNVLNANI
                     RGLVVALNAIAEKKQQASA"
     gene            3610..3999
                     /gene="rplL"
                     /locus_tag="PLG72_08280"
     CDS             3610..3999
                     /gene="rplL"
                     /locus_tag="PLG72_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ80830.1"
                     /translation="MASDKVVKLIEEVKALTVLELSELVKALEEEFGVSAAAPVAVAA
                     AAPAAGAAAAPAAEEKTEFDVILKEVGADKIKVIKVVREVTNLGLKEAKDLVDNAPKP
                     VKENVSKEEAAAIEAKFKEVGATVEIK"
     gene            4526..>6054
                     /locus_tag="PLG72_08285"
     CDS             4526..>6054
                     /locus_tag="PLG72_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ80831.1"
                     /translation="MVHPVKLGRTTRMSYSRIDEVLDMPNLIEVQKNSYKWFLEEGLR
                     EVFRDVSPIQDYTGNLVLEFPDYRLETDNPKYSVEECKERDTTYSAPLKVKVRLINKE
                     TGEVKEQEIFMGDFPLMTDNGTFIINGAERVVVSQLVRSPGLYYAKKLDKTGKALFSA
                     TIIPNRGAWLEYETDSNDVMYVRVDRTRKLPITVLVRALGYGTDAQITELLGDDERVM
                     ATIQKDSAKTQDEGLIEIYKRLRPGEPPTVESATSLLRGLFFEPKRYDLAKFGRFKFN
                     KKLSLASRIAGSKSAERIVSPVTGEILVDEDEYIDREKAEAIQNSGINSVYLNVEGKK
                     VRVIGNGTVDLKAFVDFDVSDVGISEKVRYSVLKELMDEYKDREDLKSALKENVDLLV
                     PKHITEEDIIASVNYLVHLSYGIGEDDDIDHLGNRRLRCVGELLQNQFRIGLARMERV
                     VRERMTIQDIDIVTPQALINIRPVAAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLSA
                     LG"
BASE COUNT         1783 a         1331 c         1661 g         1279 t
ORIGIN      
        1 gggaccagcg ccggcagcgt ccgtgctgcc ggtcgtagtt cctgtggcct cattcgagcg
       61 gcgtgatccg tccgcatctg tagcgcttgc agccacgaat gttatgatcg gccgcccgct
      121 gccctgcatg cagcctacga gccattagcg gtgcaagaca gttggagccg gtactataag
      181 agtcggccag caggtgtccg cgaagcagcc caaatgcaat ggtcggtcat ccgcattata
      241 caggcgcaaa tctggttcgc accatctcgc agctacgaat gccctgatcg gccacctgca
      301 gccgccatag caattcaaag gctggaaata ttaatccttg acatccttgc ggttatatgt
      361 taaattaggt attgctccgt ctcggaggtc ttttttttgt gttgtgaaat atcgatgccg
      421 catgtatcag cggcatctcc ataaataaac tgtgcaccct gcgaaaactg caaaggggct
      481 ggaggtgtta agcatgagag tcaaaatagt tatggcatgt actgaatgca aacagagaaa
      541 ttacaacacc atgaagaaca agaagaacga tcccgacagg atcgaaatga agaagtactg
      601 caaattctgc cataagcaca cggtacacaa ggaaacgaaa taatgcgcgg aggtgcacat
      661 tatgtctgag aacgcacgca catccaaacc ggccggtttt ggaagcaaac tggctaattt
      721 gggaaacagg atagccagat ttttcagaga tatcaggaat gaactcagga aggtcatctg
      781 gccgacaaaa gaacagcttg tcaaaagcac gatctccgta ttggcgatct gtttgattat
      841 tggtgccgtc atttgggtct gtgatgcggt tttcgcaaaa cttatggagt ggactctgtc
      901 aaggtaatca aggaggatat caatccttat ggctgtttat gatatgcctg atgaaccgaa
      961 atggtatgtg gtgcatacct attccggtta tgagaacaaa gtgaaggcca atttggagaa
     1021 aatagttgaa aacagaaatc tgcatgatta tatcttcgat atagtggtac ccatggagga
     1081 gcagatcgag atcaaggacg gaaggaaaaa ggccactttg aggaaggtat tcccgggcta
     1141 tgtccttgtt aagatgatca tgacggatga atcctggtat gttgtgagga ataccagggg
     1201 agtcacggga ttcgtggggc cgggatccaa gcctgtaccg ctttccgacg aggaagtgcg
     1261 caacatgggc gtcgaagagt tcgcgcctgt gctcgactac gaagtcaacg acactgtcag
     1321 ggtcattgac gggccccttg agaatttcat cggcactgtc gaggaaatca acttcgagaa
     1381 aagaaaagtg cgggtcgccg tatcgatgtt cggcagagag actcctgtgg aactggaact
     1441 gacacagata cagaagatat agagacggag atcggcagat ctctttctat atgatatgat
     1501 gagcagcgtg tttgacccgg actgcgggac agacatgttt taagaagaat tgggaggtgg
     1561 aataagccat ggcaaagaag atcataggct atgtaaagct gcagattcct gcggggaagg
     1621 caaccccggc tccaccggtt ggaccagctt taggacagca tggtgtaaac atcatgggat
     1681 tttgcaagga gttcaatgaa agaactgcga aggatgcggg tttgatcatt cctgtcatca
     1741 taaccgttta tgctgacaga tcgttttcct ttgtgaccaa gactcctccg gcggcagtgc
     1801 tcctgaagag ggcatgcaag atagaaagcg gttcaggcag gcccaacagg gacaaggttg
     1861 cgaagatctc gaaggaagag atcaggaaga tcgcagagct caaaatgccc gatctgaacg
     1921 ctgccagcat cgaagcagct atgagcatgg tagcaggtac ggccagaagc atgggtatag
     1981 tagttgaaga ctagcatggc cgcaggtggg aggaaaccga ggtttccgaa aaattcacca
     2041 caaaggggag gataatatga agagaggaaa gagatacatc gaaagcgcaa agcttatcga
     2101 tagagcaaag ttgtacgatc cggttgaagc tcttgacctg gtcaagaaaa cagccaaagc
     2161 gaagtttgac gagacggtcg aagtccatat caaactgggc gtcgactcaa gacatgcaga
     2221 ccagcaggtc agaggagccg tagtgcttcc gcatggtacc ggcaagacag tcagggtgct
     2281 cgtgttcgcc aaaggcgaca aggcgaagga agcagaggac aacggcgccg attatgtagg
     2341 cgcggaggac ctcgttgcga agatacagaa cgagaactgg ttcgactttg atgtcgttgt
     2401 cgctacgccc gatatgatgg gcgtcgtagg aaggctcgga aagatactcg gacccaaggg
     2461 cctcatgccg aacccgaagg ccggtacggt aaccatggac gttgcaaagg caatagccga
     2521 gattaaggct ggtaagatcg aatacaggct ggacaagacg aacatcatcc actgccccgt
     2581 agggaaagct tcattcgatc tggataagct caccgacaac ttccgcacgc tgctgggggc
     2641 cattatcaag gccaagccgg ctgccgcaaa gggacagtat ctcaggagcg tagtcgtgac
     2701 atcgaccatg ggacccggga taaaggtgaa tcctcagagg atagccgaat aaaaaagctt
     2761 tacatccggc agattatgta gtattataga caacggtcaa caaaaagcca gaaaatcgaa
     2821 tatgtcacat accgcagaca gcaggtgctt ccagcataat cggaaaaaaa gccggcctgc
     2881 cgaggtaagc gttgatacgc ggagccgcat gctccgttgc cgatatggtg tttcgccctt
     2941 gcatgtctgc tgtatgcaga tatacaaggg cttaatttat gcaaggaggt ggatggtttg
     3001 cctaaggaat acaccctgaa tcagaagcgt gaagtaatag acaggatcag cagcaggatg
     3061 aaggcggcaa aaacaatgat ttttgctgat tacaggggat tgacagtgga acaggatacc
     3121 gagctgaggc gtgctttgag aaacgcgggc gtggaataca aggtcgtaaa gaacacgctg
     3181 accaaattcg cagccaaaga gaacggactt gaggagctgt atccctatct gaacggacct
     3241 acgtccatgg catcaagcga tactgaccct gtggcacctg cgaaggtatt gtgcgatttt
     3301 gcaaagaagt atgagagttt cgaactgaaa gtaggcgtag tggaaggcaa ggtaatggat
     3361 gtcgacggga taaaagccct ggctgaactg ccgtcgaggg aagttctcat tgccagagtc
     3421 cttggaggtt tcaacgctcc catatccggc tttgtcaatg tactcaacgc caatatcaga
     3481 ggattggtcg tggcgctcaa tgcaatagcc gaaaagaagc agcaggcgtc cgcatgagta
     3541 atgtaagaca gtcaatataa cataaaacag gaactaaaaa ttcaattcta aatcacggag
     3601 gtatttgaaa tggctagtga taaagttgta aagcttatcg aagaagtaaa ggcattgaca
     3661 gtcctggaac tgtccgagct cgtaaaggct cttgaagagg aattcggagt atccgcagca
     3721 gctcccgtgg cagtggctgc agcagcaccg gcagcaggcg ccgcagcagc accggccgct
     3781 gaggaaaaga ccgagttcga tgttatcctc aaggaagtcg gcgctgacaa gatcaaggtc
     3841 atcaaggtcg tcagggaagt taccaacctg ggcctcaagg aagcaaaaga cctcgtggac
     3901 aacgctccca agccggtcaa ggagaacgtt tccaaggaag aagcagccgc tatcgaagca
     3961 aaattcaagg aagtcggcgc aacagtcgaa atcaaataat taccggatcg acgaaacgtc
     4021 ccggacatat ccgggaaaaa tgccccgggt atgcccgggg catttttttg gcgctaaatg
     4081 aaaaattgca ataaaaaatc accgtaaaaa cacgttgaca agtatgatgc ggtgtgctag
     4141 aattataaac tgcgttataa tctgaacttg tatgcttttt caggctgcaa ccggaatcgt
     4201 tcgcagatag attataacac aggggaataa aatacacaag aactgttaat aattatttaa
     4261 tggttttagg caatttgacg aatgaaatcg aaatcctgcc atattcatat atatctatat
     4321 atgagccgat ataaccgcga ttcagatttc tctgtcggat tgctgtgcaa tattgcccca
     4381 aaagctttca ggaatgcagc tgaaagcttt acatacgccg gctgttgtac cgataaggcc
     4441 ggcgatccgg ttgaattgca tgaatgcaag cctttcaggt aacgttgttc tttttacaag
     4501 tcataatatt atgaggtgat tattgatggt acatcccgta aaactgggcc gaactaccag
     4561 aatgagctat tccaggatag atgaggtcct ggatatgccg aatctcatcg aagtgcagaa
     4621 gaactcttac aagtggtttc tggaagaagg cctgcgcgag gtgtttagag acgtttcccc
     4681 tatacaggat tataccggga acctggttct ggaatttccg gactacagac tggagaccga
     4741 taaccccaag tacagtgttg aagaatgcaa agaaagggat actacatatt cagcaccgct
     4801 gaaagtaaaa gtccgcctta tcaacaaaga gaccggagag gtcaaggaac aggagatctt
     4861 catgggagat ttcccgctga tgaccgacaa cggtactttt attatcaacg gggctgaaag
     4921 ggtcgtagta agccagctgg tcaggtcacc cggtctttat tatgccaaaa agctcgacaa
     4981 aacggggaaa gcgctgtttt cggctaccat cattccgaac cgcggcgcat ggctggagta
     5041 cgagacggac tcaaatgacg tgatgtacgt aagggtcgac aggaccagga aactgccgat
     5101 cactgttctc gtccgcgcgc tgggatatgg tacggatgcc cagataaccg aactcctcgg
     5161 cgatgacgag agagtaatgg ccactatcca gaaggacagc gcaaaaaccc aggacgaggg
     5221 tctcatagag atatacaaga ggctcagacc cggtgagccg ccgacggtcg aaagcgctac
     5281 atcgctgctc aggggtctgt ttttcgagcc caagagatac gatctcgcca agttcggaag
     5341 gttcaagttc aacaagaaac tttcactggc ttcgagaata gccggcagca aatccgcgga
     5401 aaggatcgtg agcccggtca ccggtgaaat actggttgat gaagatgaat acatcgacag
     5461 ggaaaaagcc gaagcgatac agaactccgg catcaattcc gtatacctca acgtggaagg
     5521 caaaaaggtg agggtgatcg gaaacggcac tgtcgatctg aaagcattcg tcgattttga
     5581 cgtctcagat gtcggtatct cggaaaaagt gagatacagc gtgctcaaag agctcatgga
     5641 cgaatataag gacagggagg acctcaagag cgcactcaaa gagaatgtgg acctgcttgt
     5701 ccccaagcat atcacggaag aggatataat agcgtccgtc aattacctcg tacacctcag
     5761 ctatggtatt ggcgaggacg atgatataga ccatctcggc aacaggaggc tccgctgcgt
     5821 cggagaactg ctgcagaacc agttcaggat cggtcttgcc aggatggaga gggtcgtcag
     5881 ggaaaggatg accatacagg acatcgatat agtcacgccc caggcactga tcaatatcag
     5941 gccggtcgcc gcagccatca aagagttctt cggaagcagc cagctgtcac agtttatgga
     6001 ccagacaaat ccccttgcgg aactgactca caagagaagg ctgagcgcac ttgg
//
LOCUS       DAOSCC010000149         5981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8417_length_5981_cov_4.781809, whole genome shotgun sequence.
ACCESSION   DAOSCC010000149 DAOSCC010000000
VERSION     DAOSCC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8417_length_5981_cov_4.781809"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLG72_08290"
     CDS             <1..367
                     /locus_tag="PLG72_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ80832.1"
                     /translation="KIIMGGPMMGVSQHSLDDPVLKQTNALLAFNERDAEQPKESVCI
                     RCGKCVAACPMSLLPLYINLNVVRGKFEETEKYHVTDCIECGCCSYVCPASRNLVQSV
                     RIAKAELRKIAARKQGGKN"
     gene            369..1331
                     /locus_tag="PLG72_08295"
     CDS             369..1331
                     /locus_tag="PLG72_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOJ80833.1"
                     /translation="MEISLLAGSSPHIKSGESTQKIMRDVIIALLPAAVSSVIYFKLK
                     AAMLILVTVVSCVAAEYAWCKITKKENAVTDLSAVVTGLLLAFNLPPSLPPWMAAIGG
                     VFAIIIVKQLFGGLGHNFVNPALAARAFMVASWPSQMTSWQEPGWDAVSTATPLEIMK
                     NGSRAAEQLPKLWDMITGNIGGCIGETSALALLIGVVYLVVRKVITLELPLTFIGTVG
                     FMTWMLGGSEGLFTGNFVYHIFAGGLILGAFYMATDYTTSPVTFKGRIIMGIGCGFLT
                     SIIRLYGGYPEGVSYSILLMNLVVPVIDRYMIPKSFGGGVAANE"
     gene            1324..2190
                     /locus_tag="PLG72_08300"
     CDS             1324..2190
                     /locus_tag="PLG72_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOJ80834.1"
                     /translation="MSEERRELAQEGMHEGLQAGSPEEMQKDIREDTREENLDAVREE
                     ICEDADEAKRETNADTDEGINGEVQETPGIGAHEETPVEKRGPGGIFSDEFREMMGTA
                     LRLFVICLVTALCLAVVNDITKDTIALRDQQAAEEQRMLVMSGADSFEKLEDWEDQEG
                     TGLVKEVYAAYSGDALVGYVFSAISSGYGGDIPVTVGVGKDGTITGIRIGDNEETPGL
                     GSKVAGERFTSQYEGKDISGELKIVTGPVSADDEIQAVSGATISTRAVNSAVQASADL
                     GARLLQQNGGGK"
     gene            2190..2870
                     /locus_tag="PLG72_08305"
     CDS             2190..2870
                     /locus_tag="PLG72_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOJ80835.1"
                     /translation="MRLLKIFKNGIIDENPTFRLVLGTCPTLAITTSAVNGIGMGLAT
                     TAVLIGSNVVISLLRKFIPNKVRIPAFVTVIAGFVTIVQMLLKAYVPALDKALGIYIP
                     LIVVNCIIMARAESYASKNPVLDSAVDGLGMGIGFTIAITLIGAVREILGNGSIFNMP
                     LFGESASPALAMILPPGGFLVFGLAIGLVNLLTNRQVHAAGCGAHEAGCDACPANCGF
                     AAEEKGGQ"
     gene            2873..3469
                     /gene="rsxA"
                     /locus_tag="PLG72_08310"
     CDS             2873..3469
                     /gene="rsxA"
                     /locus_tag="PLG72_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOJ80836.1"
                     /translation="MLASILIAFISAVFINNFVVVKFLGICPFLGVSRNIKTAAGMGV
                     AVIFVMTFASSATWAVQEFLLKPLKLEYLQTIAFILVIAALVQLVEMVLQKTSQALYK
                     ALGVYLPLITTNCAVLGVCILNLEIQGNARFLMSTLNGAFSGVGFTLAIVLFAGIRER
                     LETADIPKCLEGFPISLISASLVSLAFFGFKGLFQGLI"
     gene            3607..4407
                     /locus_tag="PLG72_08315"
     CDS             3607..4407
                     /locus_tag="PLG72_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOJ80837.1"
                     /translation="MEILYAVLVISGLGLIFGIGLSYASRVFAVKVDEKVALVREVLP
                     GANCGACGYSGCDGYASAVVAGDADTNCCPVGGNEVEKKIADIMGVEPEGIKRTAARV
                     LCNGRQSVSKEKYEYQGIDSCAAAAQVFGGHKACIYGCLGHGDCVRACQFDAIIMVGG
                     IARIIEDRCKACGKCVAACPKNLIELIDKTKKYSVMCRSRDKGALTRTICQVGCIGCR
                     RCVKACPQQAIYMEHETLAKIDYDKCDNCGECTKVCPTMAIRVMDFGD"
     gene            4907..5398
                     /locus_tag="PLG72_08320"
     CDS             4907..5398
                     /locus_tag="PLG72_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOJ80838.1"
                     /translation="MSKKIWKTSDYVRYITEAAVTAALYAALTILIPGSSGQVQVRVS
                     EALTVLPFFTQAAIPGLFAGCIIANMVTTSAGVYDVVFGSLATLTAAFLTSKMPSKYL
                     APLPPVVINAVVVAAVLNATINAPLFVTMGFVALGEMIACYGLGYPLILFLEKKAGKI
                     FGR"
     gene            5558..>5981
                     /locus_tag="PLG72_08325"
     CDS             5558..>5981
                     /locus_tag="PLG72_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOJ80839.1"
                     /translation="MYNSIQHFNEFGLREIEEVIKTFVAEKKDLADFVLDLQEILYEL
                     GRSIVTEVLEDMDDYIRNSTERKKQYEIVRKDPNTLLTSFGMVSYKRTYFKPRAGGHR
                     KYLVDELAGIDPHDRVSADVVINAIEEATQSSYRKAGTK"
BASE COUNT         1598 a         1272 c         1756 g         1355 t
ORIGIN      
        1 aaagatcata atgggcggtc cgatgatggg tgtgtcccag cactcgctgg acgatcccgt
       61 attgaagcag accaacgcgc tgttggcctt caacgagagg gatgcggagc agccgaagga
      121 atcggtatgt atacggtgcg gcaagtgcgt ggcagcgtgc cccatgtctc tgctgcccct
      181 gtatatcaat ctgaacgtgg tccgcggaaa gtttgaggaa accgaaaaat accatgtcac
      241 cgattgcata gaatgcggct gctgctcgta tgtatgcccc gcatccagga atcttgtaca
      301 gtccgttcgt attgccaaag cggaactgag aaagatagca gcaaggaagc agggagggaa
      361 aaactaacat ggaaatcagt cttttagccg gttcgtctcc gcatataaaa agcggtgaga
      421 gcacgcagaa aataatgagg gacgtaataa tagcattgct gcctgcggcc gtcagctcag
      481 tgatatactt taagttaaag gccgccatgc tgatacttgt gactgtcgtc tcttgcgtgg
      541 ctgccgagta tgcgtggtgc aaaataacta aaaaagagaa cgctgtaacg gatctcagcg
      601 cagtggtgac aggcttgctt ctggcgttca acctgccgcc gtccctgccg ccgtggatgg
      661 cagccatcgg aggcgtattc gccatcatca tagtcaagca gttgttcgga gggcttgggc
      721 acaatttcgt aaatcctgca cttgctgcaa gggcattcat ggtggcgtcc tggccgagcc
      781 agatgacatc gtggcaggaa cccggatggg atgcggtcag tacggccacg ccgctggaaa
      841 tcatgaagaa cggaagcaga gccgccgaac agttgccgaa gctctgggac atgatcacag
      901 gcaatatagg cgggtgcatc ggtgagacat cggcgctggc gctgcttatc ggggttgtgt
      961 acctggtagt cagaaaggtc atcacactgg aattgccgct cacatttatc gggacagtcg
     1021 ggtttatgac ctggatgctt ggaggcagcg aaggcttgtt taccgggaat tttgtttacc
     1081 atatattcgc aggaggactt atcctgggcg cattctacat ggcaacggac tacactactt
     1141 ctcctgtaac gttcaaagga aggatcataa tggggatcgg ctgcgggttc ctgacatcca
     1201 tcatcaggct gtacggagga taccctgaag gcgtgtcata ttccatactc ctgatgaacc
     1261 ttgtagtacc tgttatcgac aggtatatga tcccgaagag tttcggaggg ggtgtggcgg
     1321 caaatgagtg aagaaaggcg tgaactggcg caggaaggga tgcatgaagg attacaggcg
     1381 ggatcgccgg aagaaatgca gaaagatata agggaagata ccagggaaga gaaccttgat
     1441 gcagtacggg aagaaatctg cgaggatgct gacgaagcaa agcgtgaaac taacgcagat
     1501 accgatgagg gaatcaatgg tgaagtacag gaaacacccg gcattggagc gcatgaggaa
     1561 acacccgtgg aaaaacgcgg accgggcggg atattcagtg atgaattccg cgaaatgatg
     1621 ggtaccgcac tgaggctttt cgtaatatgc ctggttacgg ctttatgcct tgctgttgta
     1681 aacgacataa cgaaagatac cattgccctg cgtgaccagc aggctgcgga agaacaaagg
     1741 atgctggtta tgagcggagc agacagcttt gaaaagctcg aggactggga agaccaggaa
     1801 gggacagggc ttgtgaaaga agtctatgcg gcatacagcg gagatgcact ggtcggttat
     1861 gtgttctcag ccatatcttc cggttatggc ggtgatatcc ctgtgacggt cggcgtcggg
     1921 aaagacggta cgataaccgg tatcagaata ggcgataatg aagaaacgcc gggccttgga
     1981 agcaaggtgg ccggtgaaag attcacctcg cagtatgagg gaaaggatat atcgggcgaa
     2041 ctgaaaatag ttaccgggcc ggtttcggca gatgatgaaa tacaggcggt aagcggtgcg
     2101 actataagta cgagagctgt caacagcgcc gtacaggctt ctgcagatct tggcgccagg
     2161 cttttgcagc agaatggagg cggtaaataa tgagactgtt gaaaatattc aaaaacggga
     2221 tcatcgatga gaatccgaca ttccgccttg tactggggac atgtcccacg ctggctataa
     2281 cgacgtcggc tgtgaacggc ataggtatgg ggcttgccac gacggcggtg ctgatcggtt
     2341 ccaatgtggt catttcactg ctgaggaagt tcatacccaa caaggtcagg atacctgcgt
     2401 tcgtcacggt catagcggga ttcgtcacca tagtgcagat gctcctgaag gcgtatgtac
     2461 cggcgcttga caaggcactg ggaatataca tcccgctcat cgtcgtcaac tgcataataa
     2521 tggccagggc ggagtcatat gcttcaaaga atcccgtgct ggattcggca gtggacggcc
     2581 ttgggatggg tatcggattt acgatcgcga tcactctgat aggcgcggta agggagatac
     2641 tcgggaacgg cagcatattc aacatgccgc tgttcggaga atcggctagc ccggcgcttg
     2701 caatgatact ccctcccgga gggttccttg tgttcggcct ggccataggg ttggtgaacc
     2761 tgctgaccaa caggcaggtc catgcagcag gctgcggcgc tcatgaagca gggtgcgatg
     2821 catgcccggc caactgcgga tttgccgctg aggagaaggg gggacagtaa taatgcttgc
     2881 gagtatactg atagcgttta tttcggctgt ttttataaat aatttcgtgg ttgtcaaatt
     2941 cctggggatc tgtccgttcc ttggagtatc gaggaatata aagactgcgg caggtatggg
     3001 cgtagcggtc atattcgtca tgacattcgc atcgtcggca acgtgggcgg tacaggaatt
     3061 cctgcttaaa ccgctgaaac ttgagtatct gcagactata gcatttatcc tcgtcatagc
     3121 ggcgttggtg cagttggtgg aaatggtgct gcagaagacg agccaggccc tttataaggc
     3181 gctgggtgtt tatctgccgc ttatcacgac aaactgcgcc gtacttggcg tttgcatact
     3241 caacctggag atacagggga acgcaaggtt cctgatgtcc acgctgaacg gcgctttttc
     3301 cggagtagga ttcactctgg cgatagtgct gtttgccggt atcagggaac gccttgagac
     3361 tgctgatata cccaagtgcc tcgaaggttt cccgatatca ctgatctccg cgtccctggt
     3421 atcactggcg ttcttcgggt tcaagggatt gttccaggga cttatatgag aggatggact
     3481 caaataaccg cgggattcga aattaagaca cgggtttgga tatgaagcct gaaatactga
     3541 caactgaaat gaatttttga tggccggact gattaacgat agaaaggccg gaggggtgaa
     3601 atataagtgg aaattttata tgcagtattg gttataagcg gtctcgggct aatatttgga
     3661 ataggtctgt cctatgcatc cagggttttt gcagtaaagg tcgatgaaaa agttgcgctg
     3721 gtccgggaag tacttcccgg agctaactgc ggtgcatgcg gctattcggg atgcgacggc
     3781 tacgcaagcg ctgtggtagc cggcgatgca gataccaact gctgcccggt gggcggaaat
     3841 gaagtggaga aaaagatagc agatattatg ggcgtggagc cggagggcat aaaacggact
     3901 gctgcgagag tcctgtgcaa cggacggcag agcgtaagca aggaaaaata tgagtatcag
     3961 ggcatcgaca gttgtgcagc cgcggcccag gtcttcggcg ggcataaggc ctgcatatac
     4021 ggctgcctcg gacacggtga ctgcgtcagg gcatgccagt tcgacgccat catcatggta
     4081 ggcgggatag caaggattat cgaggatcgc tgcaaagcat gcggcaaatg tgtggctgca
     4141 tgcccgaaaa acctgataga actcatagac aagacgaaga agtattcggt gatgtgcagg
     4201 tccagggaca agggcgctct gacaaggacc atctgccagg taggctgtat aggctgcagg
     4261 cgctgcgtca aggcatgccc gcagcaggcg atatatatgg aacacgagac gcttgcaaag
     4321 atcgattacg acaagtgcga caactgcggt gaatgcacga aggtctgccc gacaatggca
     4381 ataagggtca tggacttcgg ggattgatgc agagtattgc catgatgtgc atttgtcgtg
     4441 tataataaca tcatattgct gacagtatac tgaaaatgat aatttactgc ttatgtacac
     4501 ttagtttctt agagcactaa accggcgatg cacttaagtt gcagtttgcg ggttatgcgc
     4561 actgaaaact ggataaaaga cagttcgaaa ccatcctgtc tataaacaaa actacggaaa
     4621 ctccaggccg gcatacgggc cggcttgagt atagatgttt aagggggacg ggtacgtccc
     4681 tttttgtttg gggacgggtg cgtccctttt ttatttggaa cgggtacgtc ccattaaaca
     4741 ttttgaacaa gatgcaaatg aaaggaatga aacatgatac gaatgggata aagtgctgaa
     4801 gcaatcagag aacgggcatg cgatacggaa tgagaagcac ggatgcatga atgatgaaaa
     4861 gaacgcagta aagaattcga gaatgacagg agggaatcat aagtctatga gcaaaaaaat
     4921 atggaaaacg tcagactatg taagatacat cacggaagcg gcagtaacag ccgccttgta
     4981 tgctgcgctt acgatactga taccaggcag ttcaggccag gtccaggtga gagtgtcaga
     5041 agcgctgaca gtgctgccct tctttactca ggcagcgata cccgggctct tcgcaggctg
     5101 tattatagcc aacatggtaa ccacctctgc aggcgtttac gatgtcgtat tcggcagcct
     5161 ggcgacgctg accgccgcat tccttacaag caaaatgcct tcgaaatacc tggctccgct
     5221 gccgcctgtt gtgatcaatg ccgtggtcgt tgcagccgtg ctcaatgcaa cgatcaacgc
     5281 tcccctgttc gttacgatgg ggtttgtggc gctgggcgaa atgatagcct gttacgggct
     5341 gggatatcca ctcatccttt ttcttgaaaa gaaggcagga aaaatattcg gcaggtagtt
     5401 tgcggcatac cgcgcgggta ttctgatgcc tgagttagcg taaagttact gtagggagtt
     5461 cgaaggcata attaggaagg gcaatagaaa agaagatgaa atcctgttaa tatataatcg
     5521 actcaaaaaa tacactaaca aaggatgttc atcttctatg tataacagta tacaacattt
     5581 taacgaattt ggccttaggg aaattgaaga agtaataaaa actttcgtgg cagagaagaa
     5641 agatttggca gacttcgtac tggatctgca ggaaatcctg tatgaactgg ggagaagcat
     5701 tgtgacagaa gttctggagg atatggacga ttacatccgg aacagtacgg aacgcaagaa
     5761 gcaatatgag atcgtcagga aagatccaaa tactcttttg actagctttg ggatggtttc
     5821 atataagcgg acatatttta agcccagggc gggtggtcac cggaagtacc tggtggacga
     5881 actggcaggc attgaccctc atgaccgggt tagtgccgat gttgtaataa atgcgattga
     5941 agaagcgaca caaagcagtt acaggaaggc cgggacaaag g
//
LOCUS       DAOSCC010000150         5938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8500_length_5938_cov_4.305116, whole genome shotgun sequence.
ACCESSION   DAOSCC010000150 DAOSCC010000000
VERSION     DAOSCC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8500_length_5938_cov_4.305116"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..387)
                     /locus_tag="PLG72_08330"
     CDS             complement(82..387)
                     /locus_tag="PLG72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ80840.1"
                     /translation="MVVPGTFEVLDELKECGYRLAVVSNHRSWMPGYLKSVGLADYFE
                     QIIISDLVGCEKPDARIMEIALSKLGLLPKDCLYVGDHPFDVLCAKKAGLDCAWLAL"
     gene            complement(439..1140)
                     /locus_tag="PLG72_08335"
     CDS             complement(439..1140)
                     /locus_tag="PLG72_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOJ80841.1"
                     /translation="MFHALTDNNVWVEEILKDRDIALIGFADLSELDPETRLGYQFGI
                     CFAIALRMFPSTTSEPSREYYDEYKDVNARLRETSIFLAEKIKERGYNAYSLAHVKSN
                     EEYRTRLPFKTLATRAGLGWIGKSAALITKEYGNAIRLNGVLTDMPLSVGTPINESFC
                     GKCEECVRHCPGHAIKGNTWRLNMDRENLLDAFSCQKAIEDRGKVINLTEGTCGICIS
                     VCPWTKRYISRRGAS"
     gene            1345..1653
                     /locus_tag="PLG72_08340"
     CDS             1345..1653
                     /locus_tag="PLG72_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ80842.1"
                     /translation="MDLEKFPQAGKIQAKGADYVSVVTVQKWGNSLGIRIPKEAADKI
                     GIHQDSELEMLVSEDKRTITLRPKKARKKYSLEELLSQITPGNRHDEIDFGTEGRELI
                     "
     gene            1653..2036
                     /locus_tag="PLG72_08345"
     CDS             1653..2036
                     /locus_tag="PLG72_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOJ80843.1"
                     /translation="MADIPERGAFIVLNFDPQAGHEQAGRRIAFVLSPKNFNEKTGFA
                     VVCPITNQKKNYPFEVDLPVSGIPVPGSGDPITGVILTDQIKSLDWKARKIKILKNYD
                     PDDSQIEEIDAIIDECLAKIATYLT"
     gene            2069..2359
                     /locus_tag="PLG72_08350"
     CDS             2069..2359
                     /locus_tag="PLG72_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ80844.1"
                     /translation="MFIGVLQTELFISESQSLKDKRQVVRSLVDKIKNRFNAGVAETG
                     KLDSWNYCVLGLVFVSNEAGHLDSMMNSVANFVENQGSCEVTGIRTEIIPYK"
     gene            2607..3653
                     /gene="mtnA"
                     /locus_tag="PLG72_08355"
     CDS             2607..3653
                     /gene="mtnA"
                     /locus_tag="PLG72_08355"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOJ80845.1"
                     /translation="MDTLRWENGVLKIIDQTKLPTEEVELCIDNIKDCREAIKRLQVR
                     GAPAIGDAAAFGVLLGLNEVTANDFDSFYAEFLKIKDYLASSRPTAVNLFKSLERMDE
                     TVRKNRGRSIPEIKEILEEEALRIYREDIDTCHAIGINGAALLKEGMSVLTHCNAGRL
                     ATAKYGTALAPVYTALEQGIHVRVYADETRPLLQGARLTAYELMDAGVDVTLIADNMA
                     AYLMKQGKIDIVIVGCDRVAANGDTANKIGTYGLAVLAKAHNIPFYVAAPTSSVDLRT
                     PSGNEIVIEERSPEEITMGFGRQTAPDGVKVYNPAFDVTDHELITGFITEKGIIGPPY
                     DEHLKALEYQAVPS"
     gene            complement(3663..5066)
                     /gene="purF"
                     /locus_tag="PLG72_08360"
     CDS             complement(3663..5066)
                     /gene="purF"
                     /locus_tag="PLG72_08360"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ80846.1"
                     /translation="MDKLREECGIVGIFNFDTSRDVAKSLYYCLYALQHRGQESAGIA
                     TTDGHRTHFHKEMGLVHEAFNEEKLKGLKGNIGIGHVRYSTTGDSKVTNAQPLAVRYR
                     GGSITLAHNGNLVNAAELRDCLEENGVVFQTTIDSEVIVNLIARFSSEGIVNAIEQTM
                     EMVRGAYTLVVMTEDSLIGIRDPYGLRPLCLGKTEDGCVLASESCALDIIGAEFVRDV
                     EPGEIIVIDKDGLRSKRVQSKCRRASCIFEYIYFARPDSVMDGTSVYEARKNAGRILA
                     EEHPVEADLVIAVPDTSIPAAVGYAEASGIPFGEGLIKNRYVGRTFIQPEQELRETAV
                     RLKLNPLRYNVSGKRIVMIDDSIVRGTTSRQIVNSLKMAGAREVHLRISSPPVTHSCY
                     FGIDTPDREQLIGARKTPEQIAEEIGADSLGYLSVDGLVRSVGKPAEQLCLACFTGDY
                     PMEVPKTGNKKVFEYRQ"
     gene            complement(5123..>5938)
                     /locus_tag="PLG72_08365"
     CDS             complement(5123..>5938)
                     /locus_tag="PLG72_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOJ80847.1"
                     /translation="PRAGSAIAVTEAARNLVCSGAIPLAVTDGLNFGSPEKPDRFWQF
                     REAVIGIGEACRELDTQVISVNVSFYNEAETQAIYPTPIIGMVGLIEDIDKHCTMHFK
                     DEGDIVILLGETRNELGGSEYLSRIHGLEKGTVPRLNFGLEKKLQAFILSAIDKGLLK
                     SAHDVSDGGLAIALAECCMEKELGVKVKLESQLRTDAVLFSESQSRFVVSCSPEALAE
                     LEQAMVQREVPYRVLGKVAGKELVIDINDKNVIALNVKDMKEAWRGAFGCLMN"
BASE COUNT         1477 a         1536 c         1388 g         1537 t
ORIGIN      
        1 cggcttcatc ggtagtgttt ttaaaaatca ggtcaaatct ctgtccggtt ttactgagag
       61 agaatttgtt tggcttcagt tctataatgc cagccatgca caatcgaggc cggctttctt
      121 tgcacacaaa acatcgaaag gatgatcgcc gacatacagg caatctttgg ggagtaaacc
      181 cagcttgctc aatgctatct ccattatcct ggcatcaggc ttttcgcagc caaccagatc
      241 ggaaattatg atttgctcaa agtaatctgc caggcccacc gatttcaaat aaccaggcat
      301 ccatgacctg tggtttgaaa ctaccgccag cctgtaaccg cattctttga gttcatccag
      361 aacctcgaat gttccgggaa caaccaccag ttttacatcc tgatgggtac tttcctataa
      421 ctgctttata cggcatattc aactcgctcc tcttcgtgaa atataccgct tcgtccacgg
      481 gcataccgat atgcaaatgc cacatgtgcc ctcagttaag ttgatcactt ttcccctatc
      541 ctcgattgct ttttgacaac taaatgcatc cagcaggttt tccctgtcca tattcagtct
      601 ccatgtatta cctttgattg catgtccggg acagtgcctg acgcactctt cacattttcc
      661 gcaaaatgat tcattgatgg gtgttccgac agacaacggc atatccgtca gtacaccgtt
      721 gagccttatc gcgttgccgt attcctttgt gatcaatgca gccgacttac cgatccagcc
      781 gaggcctgcc cgcgttgcca gtgtcttgaa aggcagccgg gtccggtatt cttcatttga
      841 tttaacatgg gcaagcgaat atgcattata tccacgttct ttgattttct cagcgaggaa
      901 gatacttgtt tcccttagcc tggcattcac atctttgtac tcgtcataat actccctgga
      961 cggctcgctt gttgtactgg gaaacatccg aagagctatg gcaaagcaga taccaaactg
     1021 atacccgaga cgagtttcgg ggtccagttc cgacagatcc gcaaaaccta tcaaggcgat
     1081 atccctgtct ttcagtattt cttccaccca aacattattg tctgttagtg catgaaacat
     1141 aaccccggaa gacctctctg ttctttcagc ttaaatcagc cattccagca gcaaacattc
     1201 agggatgtta attatcaata atgtaataat aatattttat tctgccgctc atgtcaaagc
     1261 ttaccataat tttattgcat aagaatatat gaatcacaga aaagtatata ctatgtatat
     1321 acttttctgt gaaaaggtga tataatggat ttggaaaaat tcccgcaagc cgggaaaatc
     1381 caggcgaaag gagctgatta cgtgtctgtg gtcactgtcc aaaagtgggg aaacagtttg
     1441 ggaatacgta tcccaaaaga agctgccgac aaaattggca tccatcagga ctctgaactt
     1501 gaaatgctgg tttccgaaga caaacggacc attacattaa ggccaaaaaa agccagaaag
     1561 aaatactcgc ttgaagagct attatcacag ataactcctg gtaaccgtca cgatgagata
     1621 gatttcggaa cggaagggcg tgaattgata taatggcgga catacctgag agaggcgctt
     1681 tcatagttct gaatttcgat cctcaagccg gacatgaaca agccggaaga aggatcgcct
     1741 ttgttttgtc accgaaaaat ttcaatgaaa aaacaggatt tgcagttgtc tgtccaatca
     1801 ctaaccaaaa gaagaactac ccctttgaag tagatttacc ggtcagcggt ataccagtac
     1861 ccggtagcgg agacccgata acaggagtga ttttaacaga ccagatcaaa tcattggatt
     1921 ggaaagcccg caaaatcaag atacttaaga attatgatcc agatgactcc caaatcgaag
     1981 aaatagacgc gataatagac gaatgcctgg cgaaaatagc tacatacctt acataatcta
     2041 aatcgtttgc tgtccggggg atgcggttat gttcataggt gttttgcaga cagaactttt
     2101 catcagcgag tcccaatcgc tcaaagacaa gagacaggtc gtcaggagcc tggtcgataa
     2161 aatcaaaaac aggttcaacg ctggcgttgc tgaaaccgga aagcttgatt cctggaatta
     2221 ttgcgtactt ggccttgtat tcgtttcgaa tgaggccggc cacctggaca gcatgatgaa
     2281 ctccgtcgcc aacttcgttg aaaaccaggg ttcctgcgaa gttaccggta tccggactga
     2341 aatcattccg tataaatgac cgaattttac agtgaataac tgaatcttgc aacatcatat
     2401 cctgcggcag tatcacgtcc tttggcgaaa ttcaaaaacc gtccggaacg ctgcacaatg
     2461 ccgcctggtt tatcagacgc gcagccggtg catttcccgg cagacggaac catgccttgt
     2521 ggtataataa tggcgggcta ttctgacgaa gtctggctgt aactgcatgt ttatttttcg
     2581 gtagagtaat attgcggagt gctactatgg atacattaag atgggaaaac ggggtattga
     2641 aaataatcga ccagactaaa cttccaacgg aggaagtcga gttgtgcatc gacaacataa
     2701 aagactgccg ggaggcaatc aaacggctgc aagtccgcgg cgcgccggca atcggcgacg
     2761 cagccgcttt cggagttttg ctgggactga atgaagttac ggcaaatgat tttgactctt
     2821 tctatgccga atttctaaaa ataaaagatt acctggcatc atcaaggcca actgcggtaa
     2881 acctgttcaa atcactggaa cgcatggatg aaacggtcag gaagaacagg ggcaggagta
     2941 ttccggaaat aaaggagata ctggaggaag aagctttacg gatataccgg gaggacatag
     3001 atacgtgcca tgcaataggt ataaacgggg ccgctttgct gaaagaagga atgtccgtcc
     3061 ttacacactg caatgcaggg cgcctggcaa cggcaaaata cgggactgct cttgctccgg
     3121 tctacacagc cctggagcag ggcatccatg tgagagttta tgcagacgaa acgagaccgc
     3181 tcctgcaggg cgcaaggctt accgcatatg aactgatgga tgcaggggtc gatgttactc
     3241 ttatagcgga taatatggca gcttacctga tgaagcaggg caaaatcgac atcgtgatcg
     3301 taggctgtga ccgggtggca gccaacggcg ataccgcgaa caaaatcggt acgtatgggc
     3361 tggctgtact cgcaaaggcg cataatattc ctttttacgt tgcggcgccc acatcatcgg
     3421 tcgatttgcg cactccgtcg ggaaacgaga tcgtgattga agagcgcagt cccgaggaaa
     3481 taaccatggg attcggaagg cagactgcgc cggacggggt caaagtatat aaccctgcat
     3541 ttgacgtgac tgatcatgag cttattacag gatttattac agagaagggc attatcgggc
     3601 cgccttacga cgaacattta aaggcactgg aatatcaggc cgttccttct taaggcacct
     3661 gcctactgcc tgtactcaaa caccttttta ttgcctgttt ttggcacctc catgggataa
     3721 tcgcctgtaa agcaggccag gcaaagctgc tctgccggct tccctaccga tctgacgagg
     3781 ccgtcaacgc ttagatagcc aaggctgtca gcgcctattt cctcagcgat ctgctccggg
     3841 gttttccttg cccctatcag ctgctccctg tccggcgtat ctatgccgaa ataacaacta
     3901 tgggtgaccg gcggcgaact gatcctgaga tgtacctccc tggctcccgc cattttcagg
     3961 ctgttgacta tctgtctgct ggttgtcccc ctgacgatgg aatcgtcgat catgacgatc
     4021 ctcttgccgg agacattgta tctcaggggg ttcagcttga gcctgacagc cgtttccctg
     4081 agctcctgct ccggctggat gaacgtcctg cctacgtacc tgttttttat cagcccttcc
     4141 ccgaaaggaa tacccgaagc ttccgcatag ccgacggcgg caggaatgga ggtgtccggg
     4201 actgcaatta ccaggtccgc ctccacagga tgttcttccg ccaatatcct tcccgcattt
     4261 ttacgggcct cataaacgct tgtgccgtcc attacactgt ccgggcgggc aaagtagata
     4321 tattcgaata tgcaggaggc tctcctgcat tttgactgca cacgctttga cctgagaccg
     4381 tccttgtcta tgacaatgat ttctcccggc tccacatccc tgacaaactc tgcccctatg
     4441 atatcaaggg cacagctttc agaggccagg acacagccgt cctctgtttt tccaaggcaa
     4501 agcggcctta ggccgtaggg atccctgatg ccgatcaggg aatcttccgt cattaccaca
     4561 agggtatacg cgcccctgac catttccatg gtctgctcga tagcatttac gatgccttcg
     4621 ctgctgaaac gggcaataag gtttacgatg acctcggaat ctattgtcgt ctggaagacc
     4681 acgccgtttt cctccaggca gtccctcagt tcggcagcgt tgaccaggtt tccgttgtgg
     4741 gcaagggtta tgctgccgcc gcggtatctg actgccaggg gctgcgcatt ggtaactttg
     4801 ctgtctcccg ttgtggaata tctcacatgt cctataccta tattcccctt gagccccttt
     4861 aatttctctt cattaaaggc ctcatgcacc agccccatct ctttatggaa atgcgtccgg
     4921 tggccgtcag tggtggctat tcccgcgctt tcctgccccc tgtgctgcag ggcatacagg
     4981 caatagtaca gggatttggc cacatccctg gatgtatcga agttgaaaat ccctacgatc
     5041 ccgcactctt cccttaattt atccatgcct gccgctccgt tgacgcttag tggtccatca
     5101 ccgcgcaaag gttttccggt tcttaattca taaggcatcc gaaggctcct ctccacgcct
     5161 ctttcatatc ttttacattc aaagctatga catttttatc gttgatatcg attacaagct
     5221 ccttgcccgc gaccttgccc agtacccggt aaggcacttc ccgctgtacc attgcctgtt
     5281 caagctctgc cagcgcttcc gggctgcagg agaccacaaa ccttgactgg ctttcagaga
     5341 acagtacggc atccgtacga agctgggatt ccaactttac ctttacaccc aattcttttt
     5401 ccatgcagca ttccgccagg gctatggcca aaccgccgtc gcttacgtca tgggcggatt
     5461 tcaggaggcc tttgtcaata gctgacaata tgaaggcctg cagctttttt tcaaggccga
     5521 agttcagcct cggtaccgtg cccttctcaa gcccgtggat cctcgagagg tactcgctgc
     5581 cgccaagctc attcctggtc tccccaagaa gtatgacgat atctccttca tccttgaaat
     5641 gcattgtaca gtgcttgtca atgtcttcta tgaggcccac catgccgatg atgggggtcg
     5701 ggtaaatggc ctgcgtctcg gcttcgttgt agaagctgac gtttacgctt attacctgcg
     5761 tgtccagttc cctgcatgct tcgccgatac ctattaccgc ttccctgaac tgccagaacc
     5821 ggtcgggctt ttccgggctg ccgaagttga gcccgtcggt gacggccagc ggtatcgctc
     5881 cgctgcatac gaggttcctc gcggcttctg tcactgctat ggcgcttccc gccctcgg
//
LOCUS       DAOSCC010000151         5850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8666_length_5850_cov_4.065746, whole genome shotgun sequence.
ACCESSION   DAOSCC010000151 DAOSCC010000000
VERSION     DAOSCC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8666_length_5850_cov_4.065746"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1370
                     /locus_tag="PLG72_08370"
     CDS             78..1370
                     /locus_tag="PLG72_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOJ80848.1"
                     /translation="MDILYQDFLKNTFGIDESIARLAQSVEHEVRDIFGEIESVREAN
                     QYKVIAAMQEHNLSDSHFGGTTGYGYNDRGREVLDSVYASVFGAEDALVRHQIVSGTQ
                     AISICLYGNLRPGDELLSITGKPYDTLEEVIGIRGEGAGSLKDFGITYRQLDLLEDGS
                     IDMDNIKTVMGPRTKMVLIQRSRGYEWRTSIKTGDIKKAAEAVKKINKDTIVMVDNCY
                     GEFTETEEPTQTGADLVAGSLIKNPGGGLVPAGGYIAGRKELVRNAAFRLTTPGLGKE
                     VGATLGNNRLMFQGLFMAPHTVAESLKGAVFTAALMQKLGFETSPGPAEKRSDIIQAI
                     RFNDPESMIAFCQGIQKGSPVDAFVTPEPWEMPGYDSKVIMAAGAFIQGSSIELSADA
                     PLKPPYIAYMQGGLVYEHVKLGVMTALKYMKERNLVRF"
     gene            1378..2721
                     /locus_tag="PLG72_08375"
     CDS             1378..2721
                     /locus_tag="PLG72_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ80849.1"
                     /translation="MRKIYTEDEEKKTIEFPTGKALSQEEAESGRKKAFKSITVGTFL
                     ILFTVLYVPSLLNWVSGNHIAQDIIRNGVIEEYVQAQAVLIRNEVLLKPSSIDGICIP
                     EVTEGEKAAAYSTIAKVMGDESNELLREIEDINAKIIKARMEQAEKTEFFSADLARLD
                     DEIGKKVTELIAACNSRSFEEMGRKRSEIRSIVEKKAEIAAGDTKDEYINTLQKQKRT
                     IQGMIQQNTVEVRTNTSGIVSYCIDGYEDILTPDRVRDLTAAELDGIRERCASLAADT
                     RNVKKDVPFAKVIKGIDMYMAADIPAVKAKKLEEGDSVRVRISSMGFETSATIMSIGN
                     ADNDKVVVIVRMNRGLDVLSSKRVVDVDFITKTEEGLKVPLKCLRDITADGSRGRIML
                     VKSNIAVSRIVDIVCSDDEYAIIKTPEGEYERTVDLYNIYLINPDNIEEGEIIIK"
     gene            2718..3422
                     /locus_tag="PLG72_08380"
     CDS             2718..3422
                     /locus_tag="PLG72_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ80850.1"
                     /translation="MTGDRDIIIKNIDELRSRVAAAAARAGRDPAGIKIVAVTKTVEP
                     GRIMTAYQCGITDFGENRVQELVQKADIMNVECKWHMIGHLQTNKVKYIIDRVCMIHS
                     LDSIELAYEINRRAEKAGRIMDVLVQINVAGESTKYGIEPASAPDFVKEVSRMGNIRI
                     KGLMTIAPLVSDPEEVRWVFRGLRQLLIDIGKENIDNVSMEYLSMGMSNDFEVAIEEG
                     ANIIRPGTAIFGKRKS"
     gene            3493..3945
                     /locus_tag="PLG72_08385"
     CDS             3493..3945
                     /locus_tag="PLG72_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOJ80851.1"
                     /translation="MAKLLNKMLNLVGWETEEEEEEFEEEEVEEKHQPPQYLQTFTKK
                     PQGSKVVNIHSNSQFKVVIMQPESFDDAREVCDQLKSKKPVVVNLESLTKETAQRAID
                     FLSGSVYALDGDIQKISSGIYMIAPSNVDIMSNFRDELRTKATFPWVK"
     gene            3978..4250
                     /locus_tag="PLG72_08390"
     CDS             3978..4250
                     /locus_tag="PLG72_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOJ80852.1"
                     /translation="MRILLYRLARLLFDAAELILLMRVVISWLPVNRDHKLVILLYRI
                     TEPVLMPIRSMIRRSSAGQRLFFDFSPLIAFILLAILRNIVLRIIL"
     gene            4279..4752
                     /locus_tag="PLG72_08395"
     CDS             4279..4752
                     /locus_tag="PLG72_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ80853.1"
                     /translation="MNYTPNDLQNLTFKKSFMGYSEDMVNDVLDKIIEDYTAYIRENV
                     ELKDKLALLNEGLQHYKTMEEQLQNTLLVAQQTGEEIKKNAYEKAENIIREAELRAQK
                     LINDANQEVVKIKFEYEEARKKLHLFKSKCEMLLMSQLEILRQMFDEEDEESEEE"
     misc_binding    4810..5130
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5207..>5850
                     /locus_tag="PLG72_08400"
     CDS             5207..>5850
                     /locus_tag="PLG72_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ80854.1"
                     /translation="MYKKVSTDLNFVEREKEVLRYWKENKIFEKSLKFREGNETFTFY
                     DGPPTANGKPHIGHIITRTMKDLFPRYKSMKGYNVLRKAGWDTHGLPVELEVEKSLGI
                     NGKPQIEEYGVEPFIQKCKESVWKYKREWEEMSERVGYWADMENPYITYSNSYIESVW
                     WALRQIWDKGLLYKGHKIVPYCPRCGTSLSSHEVAQGYKDVKEASIFVKFKVKG"
BASE COUNT         1810 a         1173 c         1664 g         1203 t
ORIGIN      
        1 gccggtagta ttaagaatca actaatagcc taagcaaacc gataacggta ttgggaaatt
       61 accaaagaga ggtacatatg gatatacttt atcaggattt tttgaaaaat acctttggga
      121 tagacgaaag tattgccaga ctggcccaga gtgttgaaca tgaagtcagg gatatcttcg
      181 gagaaataga atccgtcagg gaagcaaacc agtataaggt catagcagcc atgcaggagc
      241 acaacctcag cgactcgcat tttggcggaa cgacaggcta tggctataat gacaggggca
      301 gggaagtgct cgacagcgta tacgcgtccg tattcggcgc agaagatgcg ctggtcaggc
      361 atcagatcgt gtcaggcacc caggcaatat cgatatgcct ttacggcaac ctgaggcctg
      421 gtgatgaact cctttccatt acgggaaaac catatgacac attggaggaa gtaataggca
      481 tcaggggcga aggcgccgga tccctgaaag attttgggat aacataccgg cagctggacc
      541 tgctggaaga cggaagcata gatatggaca atatcaaaac ggttatggga ccgcggacga
      601 aaatggtact tatccagcgc tccaggggct atgaatggag aacgtcgata aagaccgggg
      661 atataaaaaa agctgccgag gcagtaaaga aaataaacaa agatacgatc gtaatggtgg
      721 acaattgcta cggtgaattc actgaaacag aggagcctac acagaccggc gccgatcttg
      781 tggcaggctc gctgataaag aacccgggag gcgggcttgt accggccggc ggatatatag
      841 cgggcagaaa ggaactggtg cggaacgccg ccttcaggct gaccacaccg ggacttggca
      901 aagaagtagg ggcgacgctg gggaacaaca ggctcatgtt ccaggggctg ttcatggcgc
      961 cgcatacggt ggcggagagc ctcaaaggag cagtatttac agctgcgctg atgcagaaac
     1021 tgggctttga gacttcgcca ggacctgctg aaaaaagaag cgacatcata caggcaatac
     1081 gtttcaatga tcctgaaagc atgatcgcat tctgccaggg catacagaag ggatcgcccg
     1141 tcgatgcgtt cgtaactccc gaaccatggg aaatgcctgg atatgacagc aaggtcataa
     1201 tggccgcagg cgcattcata cagggatcat ccatagaact gagcgccgat gcgccgctca
     1261 aaccaccgta tatagcatat atgcagggcg gactggtgta cgagcatgtg aagcttggcg
     1321 taatgacagc tcttaaatat atgaaggaaa gaaaccttgt aaggttttag atcggatatg
     1381 aggaagatat ataccgaaga tgaagagaaa aaaacgatag aattcccgac cgggaaagcg
     1441 ttaagccagg aagaggcgga atccgggagg aagaaagcat tcaaaagcat cactgtggga
     1501 acgttcctca tcctgttcac tgttctttat gtcccgtctc tcctcaactg ggtctcgggg
     1561 aatcatatag cccaggatat aataaggaac ggcgtaatcg aggagtatgt ccaggcacag
     1621 gcagtcctta tcaggaacga ggtgctgctc aagccgtcgt ccatcgacgg gatctgtatc
     1681 ccggaagtaa ctgagggcga gaaggcagcc gcatacagta cgatagcaaa ggtgatgggc
     1741 gatgaatcca atgaactgct cagggaaata gaagacataa atgccaaaat cataaaagcc
     1801 cgcatggaac aggcggaaaa gaccgagttt ttttcagcgg acctggcaag actcgatgac
     1861 gagataggaa agaaggtaac ggaactgatc gctgcatgca attccaggag ctttgaggaa
     1921 atgggcagaa aaaggtcgga aatccgcagt atagtagaaa aaaaggccga gatcgcggca
     1981 ggcgatacga aggatgaata tataaatacg ctgcagaagc agaagcggac cattcagggt
     2041 atgatacaac agaacacggt cgaggtcagg acgaacacgt caggcatagt ttcatattgc
     2101 attgacggat atgaggatat attgacgcct gacagggtca gggatctgac agcggctgaa
     2161 ctggacggca taagggaaag atgcgcatcg ctcgctgctg atacgcgaaa tgtaaaaaag
     2221 gatgtgccct ttgccaaggt aataaaaggc atagatatgt atatggcagc ggacatcccc
     2281 gcagtcaaag cgaagaagct ggaggaaggc gacagtgtca gggtgcggat cagcagtatg
     2341 gggttcgaga catcagcgac gataatgagt ataggtaatg cggataatga caaggtcgtc
     2401 gtaatagtca ggatgaacag aggtcttgac gtcttatcaa gtaaaagggt ggtagatgtc
     2461 gatttcataa caaaaaccga ggaaggcctg aaggtaccgc tgaaatgcct cagggatatt
     2521 accgccgacg gttcaagggg caggataatg cttgtgaaaa gcaatatcgc tgtcagcagg
     2581 attgtcgaca tcgtatgcag cgatgacgag tatgcgatca taaagacgcc tgaaggagaa
     2641 tatgaaagga cagtggacct ttacaacatt tatctgataa atccggataa tatagaagaa
     2701 ggtgaaataa taataaaatg acaggggatc gtgacatcat aatcaaaaat atcgatgagc
     2761 tccgttcaag ggtggcggcg gccgcggcca gggcgggacg ggaccccgcc ggcataaaga
     2821 tagtcgccgt caccaaaaca gtggagccgg gacgtataat gacggcatac caatgcggaa
     2881 taaccgattt tggtgaaaac agggttcagg aattagtgca gaaggccgat ataatgaatg
     2941 tagaatgcaa atggcacatg atcggccacc ttcagacaaa caaggtgaaa tatatcattg
     3001 acagggtatg catgatacat tcgctggaca gtatagaact ggcctatgag atcaacaggc
     3061 gggctgagaa ggccggcagg atcatggatg tgctcgtgca gatcaatgtg gccggagaaa
     3121 gcaccaagta tgggatagag cctgcatctg ctcccgactt cgtgaaagaa gtcagcagga
     3181 tgggaaatat caggataaaa ggattgatga caatagcgcc gctggtctcc gatccagaag
     3241 aagtaaggtg ggtattcagg ggcctgcggc aattgcttat tgacatcgga aaggaaaata
     3301 ttgataatgt aagtatggaa tacctatcta tggggatgag caacgatttt gaagtcgcca
     3361 tagaagaggg agcgaatata ataagaccgg gaacggcgat tttcggaaaa cgtaagagtt
     3421 agcgaaggtt gttacggatg ccggctcaaa ggagtgggcc ggccaacgaa agaaaaacgg
     3481 agggatatac taatggcaaa gcttcttaac aaaatgctga atctggttgg ctgggaaacc
     3541 gaagaggaag aagaggaatt cgaggaagag gaagtggaag agaagcatca gcctcctcag
     3601 taccttcaga cctttaccaa aaaaccacag ggcagcaaag tagtaaatat ccattccaac
     3661 agtcagttca aagtcgtcat tatgcagcct gaatcatttg atgacgccag ggaagtctgt
     3721 gaccaactga agagcaaaaa gccggtcgtc gtcaatcttg agagtctcac gaaggaaaca
     3781 gcgcagaggg ccatcgattt tctcagcggt tcggtgtatg ccctggatgg cgacattcag
     3841 aagatatcgt cagggatata catgatcgcg ccgagcaatg tggatatcat gagcaatttc
     3901 agggatgaac tcaggaccaa agctacattc ccatgggtaa aatgatctgt ctgccgtttt
     3961 aggtttggag gcgtgtaatg aggattttgc tgtacaggct ggccaggctg ttatttgacg
     4021 cggcggaact gatactgctg atgagggtcg tcatttcatg gctgcctgta aacagggacc
     4081 acaagctcgt aattttgtta tacaggatca ctgagccggt tttaatgccc ataaggtcga
     4141 tgatcagaag gtcgtctgca gggcagcggc tgttttttga tttttcgcca ctgatagcat
     4201 ttatactgct tgccattttg cgtaatatag ttttgcggat catattatga tattgtatgg
     4261 acaaggggtg agcattccgt gaattataca cctaatgacc ttcagaacct gactttcaag
     4321 aagtctttca tgggttacag cgaggatatg gtgaatgatg tcctcgacaa gataatcgag
     4381 gattataccg catatataag agaaaacgtt gagctcaagg acaagcttgc actgctcaac
     4441 gaaggcctgc agcactacaa gacgatggag gagcagctgc agaacacgtt gcttgtggcg
     4501 cagcaaaccg gagaagagat aaagaaaaat gcctatgaaa aggcggagaa catcatcagg
     4561 gaagctgaat tgagagcaca gaaacttatc aatgacgcga accaggaagt agtcaagatc
     4621 aagttcgaat atgaggaagc gaggaagaaa ctccatctgt tcaaatccaa gtgcgagatg
     4681 ctgctgatgt cgcagctgga aatcctcagg cagatgttcg acgaagagga cgaagaaagc
     4741 gaagaagagt gaatttgttg acagcaggcc ggcattattg tataatgctt ttcaatattc
     4801 tgaataactg caaatactgt gatcggaacg agtaaaggac ctgaacgctt ccagagagcg
     4861 gacggctggt gggagtccgc agcaacggtt cttgaaaatc atccgggagt agctgtcgga
     4921 aagcagagcc gggatcaggc ccggcgcttc ggagcctcgg gatcggactg cgagtatgat
     4981 aagccggttc acaccgttat atgtggaacg agtgactgct gcagagtacc ggacaacgat
     5041 gccggcatgt ctgcctgtta taggctggtc cggatacggc agcggtaaat tgggtggtaa
     5101 cgcgtgagat gttatgctct cgtccctttg catgggacgg gagcttttta gtttaaaaga
     5161 gctttttctt aaaacaggaa cgtaatgtga tagcgaggag gaaaccatgt ataaaaaggt
     5221 atctacagat ctcaactttg tcgaacggga aaaggaagtg ctaagatact ggaaggaaaa
     5281 caaaatattc gaaaagagcc ttaaattcag ggaaggcaat gaaaccttca cattctatga
     5341 cggtccgccc acggcaaacg ggaagcctca tataggccac atcatcaccc gtaccatgaa
     5401 ggacctcttc cccaggtaca aaagcatgaa gggctataat gtcctccgta aggcagggtg
     5461 ggacacccac gggctgcctg ttgaactgga agtggaaaag tccctcggga tcaacggaaa
     5521 accgcagatc gaggagtatg gtgtagaacc gttcatacag aaatgcaagg aaagcgtatg
     5581 gaagtataag cgcgaatggg aagagatgag cgaacgtgtc ggttactggg ctgacatgga
     5641 gaacccctac atcacgtaca gcaactcgta tatagaatcc gtgtggtggg cgctcagaca
     5701 gatctgggac aagggactcc tgtacaaggg gcataagata gtgccctact gtccgcgctg
     5761 cgggacatcg ctctcgagcc acgaggtagc gcagggatat aaggatgtca aagaagcgtc
     5821 gattttcgtc aaattcaagg tcaagggcga
//
LOCUS       DAOSCC010000152         5835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8706_length_5835_cov_4.713668, whole genome shotgun sequence.
ACCESSION   DAOSCC010000152 DAOSCC010000000
VERSION     DAOSCC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_5835_cov_4.713668"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLG72_08405"
     CDS             <1..208
                     /locus_tag="PLG72_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOJ80855.1"
                     /translation="FEKAMGSLEGVTLESVVIAKRTASALKKRIEKGLASTADFPLLL
                     HIDDIISNGAVADIPWKSFETEVF"
     gene            357..947
                     /locus_tag="PLG72_08410"
     CDS             357..947
                     /locus_tag="PLG72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ80856.1"
                     /translation="MSYNSYIYGRWIKIGIAALVIIILAATSISTYNGLVAADQQVQK
                     AWSTVESMMQRRADVINNQVETVKGYMQHEEKVISEIAAARAVLYDNYSDINSKIAAD
                     EKISAAGRQILALAENYPELKAGDLFENLQVEIEGSENRVSVARKDFIDAVQVYNTKV
                     SRFPGNIFARLMGFEKKEYFKASPGAQEAPKVDFGS"
     gene            991..1890
                     /locus_tag="PLG72_08415"
     CDS             991..1890
                     /locus_tag="PLG72_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOJ80857.1"
                     /translation="MKKKTAAIITIIIISLLLAPGSCAAGDYPSATGEFFVNDFADVL
                     DQDTERSIVQIGMALEELTTAQVVVVTIDTLDGRDIDSYANELFRKWGIGQKDKNNGV
                     LILDAVQDRMLRIEVGYGLEGALTDIETAKIRKEYMNPYLSQGDYDSGLLKGYIAVVN
                     EVAAEYGISMDDISQKLPSESRMDYNFYSQYPSDGGHNGGGDGFLPTFAVIVMFLAVD
                     GIFFRFRITEMLLRIAFYGSLFGGRHRSRRRRGGWDDDDFGGFGRGGFGGFGGGGHGG
                     GGFGGGSHGGGGSSGGGGSSGSY"
     gene            2046..4427
                     /locus_tag="PLG72_08420"
     CDS             2046..4427
                     /locus_tag="PLG72_08420"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOJ80858.1"
                     /translation="MKRLFALFLAAVIVLAGTGSYSQADVVPEVAIWLEGEKIVFPDA
                     QPIHTGTRVLVPVRGFFEKIGVTVDWNAANRMVILKDGSREIMMKADSRAVLNNGAVE
                     YLDSSVIVRGGRAFIPIRYISESFGYKVEWDGKTKSVYVTKAPEDTQGKTDDASALPT
                     LGDLESFYYLLKYNGYMADYLDIYDYSPGIRVLPKLSESAASETWTTTPDQAADAPDG
                     ADYSGTNVQVEGVDEGDVVKTDGKYIYLADGSELKIINADPQDLRIISRIPYRGSISE
                     IYIHQDRLVIISGDSRIECRARDSREQALLDNLLGRIGINSTNVKVYDVSDRARPVLV
                     SDKDYEGRYLSSRLTDDDLYIVTNSRVRFFDSYFTDKVSEILATNDKNRFIKLMGEDT
                     AQAKSLLQLAGDRDGAELFDDLRNLVEEYITPRSVDNKTGKTRVISLKDVYCFRDMVR
                     PNYMITVGLDLDSGREEVKAYLGSSGSMYVSQDHLYAAVAAYEYNVLKSRLEGYPYYD
                     YLTTVYRFGLDDGRIAYEAKGKVPGEILNQFSMDEYKGIFRIATSSRETFGSTTNNVY
                     ALDKDMKITGKLEGIAEGERIFSTRFAGERIYMVTFREMDPFFVIDAADPQKLKVLGY
                     LKIPGFSTYMHILDRDHVLGFGYETEERAGWGFDTTGFKVSLFDVSDVANPVEAKKEV
                     IGKKAESPLSTDHKALMISPEKGIMGFPVNYITSDSDYFAGYHLYSISRGDFKLKGRV
                     THVPDGTPMNGIREEDLIYRGLYIGNNLYTVSDGQLQVHDLATLDKKGSLSLK"
     gene            4660..5373
                     /locus_tag="PLG72_08425"
     CDS             4660..5373
                     /locus_tag="PLG72_08425"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOJ80859.1"
                     /translation="MSGVEVLRKWVEKCNNIVFFGGAGVSTESGIPDFRSVDGLYNQK
                     YKYPPETILSYSFFIRNQEEFYKFYRDKMLFLDAEPNITHKKLAQLEAEGKLKAVITQ
                     NIDGLHQKAGSRKVLELHGSVLRNYCMRCGKSYGVETIIEAKGVPRCSCGGVIKPDVV
                     LYEEGLDQDVLSESVRLISEAEMLIVGGTSLSVYPAAGLVRYYRGDKLVLVNKSPTPY
                     DRYADLLLQCGLGEVFSKI"
     gene            5509..>5835
                     /locus_tag="PLG72_08430"
     CDS             5509..>5835
                     /locus_tag="PLG72_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80860.1"
                     /translation="MYDIIIVGGGPAGSTLARLAGDRHRVLLLEKRSFAEDMSFASQK
                     CCGGLLDPDAQRMLAKFGLGIPKNVLMSPQLFAVRTIDIKNRIERYYQRHYINIDRSA
                     FDKWLES"
BASE COUNT         1554 a         1285 c         1732 g         1264 t
ORIGIN      
        1 gtttgaaaag gcgatgggat cactggaggg agtaacactg gaatcggtcg tgatagcaaa
       61 gcgcaccgca tccgcgctga agaagcggat cgaaaaaggt ctggcatcca ctgccgattt
      121 tccattgctg ctccacatcg atgatatcat aagcaacgga gcggtggcgg acatcccgtg
      181 gaagagcttt gagacggagg tattttgaga caggctgcag aactgccggc ctgttgccgg
      241 caaatcgcat ggttatcaga caacttggca gccggatgcg tttaacatat aggcggacat
      301 agcaggatat aattcagtaa atgacgttat aaatgaaaag gggtggttcc atgagcatga
      361 gctacaattc ctatatctat ggaagatgga tcaaaatcgg catagctgcg ctggtgatca
      421 tcatactggc ggcaacttcg atatccacat ataacggcct tgttgccgcg gaccagcagg
      481 tccagaaggc atggagcaca gtcgagagca tgatgcagag aagagccgac gtgatcaaca
      541 accaggtcga aacagtgaaa ggctatatgc agcatgagga aaaggtgatc agtgaaatag
      601 cggcagccag ggcggtactg tatgataatt actcggatat caacagcaag atcgcggccg
      661 atgaaaagat ttcggcagcc ggaaggcaga tcctggctct ggccgagaat taccccgagc
      721 tgaaagccgg cgatctgttc gaaaacctgc aggtggagat cgaaggttcg gagaacaggg
      781 tgtccgtagc caggaaggac tttatagatg cggtccaggt atacaacacg aaagtatcga
      841 gattcccggg gaatattttc gcaaggctga tgggttttga gaaaaaagag tatttcaagg
      901 catctcccgg cgcccaggaa gccccgaagg tcgatttcgg gagctaagtg ctgatcagga
      961 aggcttgttg ttggaaagcg caggtatgag atgaagaaaa agacggcggc tatcattaca
     1021 attataataa tttcattgtt gctggcgccc ggcagttgtg ctgcaggcga ttatccgtcc
     1081 gcgaccggcg agttcttcgt caatgacttt gccgacgtcc tggatcagga tacggaacgg
     1141 agcatcgttc agatcggaat ggcgcttgaa gaactcacta cggcgcaggt ggttgtcgtg
     1201 acgatcgata ctcttgacgg ccgggatatc gacagctatg ccaacgagtt gttcaggaag
     1261 tggggtatag ggcagaagga taagaacaac ggcgtgctga tcctcgatgc cgtgcaggac
     1321 aggatgctca ggatcgaagt cggctacggg ctcgaaggcg cgctgacaga tatcgagact
     1381 gcaaaaatca gaaaagaata tatgaatccg tatctttcac agggcgatta tgacagcggt
     1441 ttactgaaag gatacatcgc agtcgtcaac gaggtcgctg cagaatacgg catatcgatg
     1501 gacgatatca gccagaagct tccgtcagaa agccggatgg attataactt ctacagccag
     1561 tacccgtcgg atggcgggca taatggcgga ggcgacggtt ttttgccgac ttttgcggtt
     1621 attgtcatgt tcctggctgt cgacgggata tttttcaggt tcaggatcac ggagatgctg
     1681 ttgaggatag ctttctacgg cagccttttc ggcggcagac atcggagccg caggaggcgc
     1741 ggcggatggg acgacgatga tttcggaggc ttcggcagag gcggctttgg cggtttcggg
     1801 ggcggaggcc atggaggcgg cggcttcgga ggcggaagcc acggcggcgg cgggagttcc
     1861 ggcggcggcg gcagcagcgg cagttactga aacggcagat acttacagag aacgtaatga
     1921 agctctgata ctgaagcggc agtcatcgat acagggcgca ccgaaacggc acatacgtca
     1981 tatcggaaca tttcgtggga aaattcgtca gatggttgtg taatacgatg tatggaggga
     2041 acactatgaa aaggctgttc gctttatttc ttgctgccgt gatcgtactg gccggcacag
     2101 gcagctatag ccaggccgac gttgtacccg aagttgccat ctggctggaa ggagagaaga
     2161 tcgtattccc ggacgcgcaa ccgatccata cgggcacgag ggtgcttgtc cctgtgcggg
     2221 gctttttcga gaagatcggt gtgacagtag actggaatgc cgcaaaccgg atggtcattc
     2281 taaaggatgg cagccgggaa ataatgatga aagctgacag cagagcggtt ctgaacaacg
     2341 gagccgtgga atatctcgat tccagcgtta ttgtgagggg cggccgcgct ttcataccca
     2401 tcaggtacat atccgagagt ttcggctata aagttgaatg ggacggaaaa acaaaaagcg
     2461 tatatgtgac caaagcgccg gaagatacgc agggcaagac ggacgatgcg tctgcgctgc
     2521 caacgcttgg tgatttggag agcttttatt atctgctgaa atataacggt tacatggcgg
     2581 actacctgga catttatgat tattctcccg gcatacgggt cttaccgaag ctttctgaaa
     2641 gtgctgcttc cgagacttgg acgaccactc ctgaccaggc ggcggacgca ccggatggcg
     2701 ccgattactc cgggacgaac gtccaggtgg aaggtgtcga tgaaggcgat gttgtcaaga
     2761 cagacgggaa atatatctat cttgcggatg gcagcgaact taagataata aacgcagacc
     2821 cgcaggacct ccgcatcatc tcaaggatcc cgtacagagg gagcataagt gagatctata
     2881 tccaccagga caggctggtc attatcagcg gcgattcgcg gatcgaatgc agagcccgtg
     2941 acagcaggga gcaggccctc ctggataact tgctgggacg cattggtata aacagcacta
     3001 atgtgaaggt gtacgatgtg tccgacaggg caagacccgt acttgtaagc gataaggatt
     3061 acgaaggccg gtatctctca tcaaggctca cagatgatga cctgtacatc gtgaccaact
     3121 cccgtgtaag gttttttgac tcctatttca ccgataaagt atctgagata ctggcaacca
     3181 acgataaaaa ccggtttatt aagttaatgg gagaggatac cgcgcaggcg aaatctctgc
     3241 tccaactggc aggagacagg gacggcgcag agcttttcga tgatctgaga aacctggtcg
     3301 aagaatatat aactcccagg tctgtcgata ataaaacggg caagaccagg gtgataagcc
     3361 tgaaagatgt atactgcttc agggatatgg tacgccccaa ttatatgata actgtcggcc
     3421 tggacctgga cagcggccgg gaggaagtaa aggcatacct gggaagctcc ggatcgatgt
     3481 acgtatccca ggatcatcta tatgcggcgg tggcggcata cgaatacaat gttctgaaaa
     3541 gcaggctgga aggatacccg tactatgact acctgacgac cgtttacagg ttcggcctgg
     3601 atgacggcag gatagcgtat gaagcgaagg gaaaggtccc cggggagata ctcaaccagt
     3661 tttcaatgga tgagtataaa gggattttca ggatagcgac ctcatcgagg gagacgttcg
     3721 gttcgacaac caacaatgta tacgcgctgg ataaagacat gaagataacc gggaagctcg
     3781 aaggcatagc tgaaggcgag agaatcttca gcacgcgctt cgccggtgaa cggatctata
     3841 tggtgacctt ccgcgagatg gatccgtttt tcgtgataga cgccgcggat cctcaaaaac
     3901 tgaaagttct tggctatctg aagatccccg ggttcagcac atatatgcac atactggaca
     3961 gggaccatgt gctcgggttc ggttatgaga ccgaggaaag agccgggtgg ggattcgata
     4021 ctaccggttt caaggtttcg ctcttcgatg tgtcggatgt ggcgaacccg gtggaagcca
     4081 aaaaggaagt gatcggcaaa aaggcagagt cgccgctttc caccgaccac aaggcgctga
     4141 tgatatcgcc ggaaaaggga ataatgggct ttccggtcaa ttatataacc tctgattccg
     4201 attattttgc cggataccat ctgtacagca tttcaagagg cgatttcaag cttaaaggcc
     4261 gtgtgacgca tgtgcctgac ggtaccccga tgaatgggat cagggaagaa gatctcattt
     4321 acagggggct ctatataggg aacaacctct ataccgtttc cgacggacaa ctgcaggtcc
     4381 atgaccttgc cacgctggat aaaaaaggga gcctcagcct gaagtaacgg ctgcagaaaa
     4441 agcatgcatg ttaaggccgg gtgttatgag ggcataaatg cctgttatat cagcccggcc
     4501 tccaatcgtt ttacgcagtt attgccgcgg ctccggcatg caatgatttg attgggcata
     4561 ctgttagtgg tagtatatag ctgtggatgg ttgccacgtc atgtgtaccg gcaaaatgtg
     4621 atcagatgtg atcagtacat ttgcaaggga gatgaaaata tgtccggtgt ggaagttttg
     4681 cgcaaatggg tggagaaatg caataatatc gtttttttcg gcggagccgg cgtgtccact
     4741 gaaagcggga tacctgattt ccgcagcgtc gacgggcttt acaaccagaa gtacaaatat
     4801 cctcccgaga cgatcctgag ctattccttt ttcataagga accaggaaga gttttataag
     4861 ttttaccggg acaagatgct gttccttgac gccgagccca acatcaccca taaaaagctg
     4921 gcgcagctgg aggccgaggg aaaactgaag gctgtcatca cgcagaatat cgacggcctg
     4981 catcagaagg ccggcagcag gaaagtgctg gagcttcatg gctctgtgct gaggaattac
     5041 tgcatgagat gcggcaagtc atacggcgtc gaaacgataa tcgaggcgaa gggtgtgccg
     5101 cggtgcagct gcggcggcgt aataaagcct gatgtcgtgc tgtacgagga agggcttgac
     5161 caggatgtgc tcagcgaatc tgtccggctc ataagtgagg ccgagatgct gatagtcggc
     5221 gggacatcgc tttcggttta tcctgctgcc gggcttgtcc ggtattacag gggagataaa
     5281 ctggtcctgg taaacaagtc tcccacgcca tatgaccggt acgcagacct gctgctgcaa
     5341 tgcggcctcg gggaagtgtt ctcaaagata tagcaaaggc agccgtgtga aaggcagatt
     5401 acgcacacca agggaatatg gccggcacat gcggttaaaa aatgtcatta taatgcggaa
     5461 acaaggtata attgtgacca gatgcaagat atactgcagg ggtgaaggat gtacgacata
     5521 atcatcgtcg gaggcgggcc cgccggttcg acgctggcca ggctggccgg agacaggcac
     5581 agggtgctgt tgctggagaa gagatcattt gcggaggata tgagtttcgc aagccagaaa
     5641 tgctgcggcg gattgctgga tcccgatgcg cagcgtatgc ttgcgaaatt cggccttggc
     5701 ataccgaaaa atgtattgat gagcccgcag ttgtttgcgg tccgcacgat cgatatcaaa
     5761 aacaggatcg agcgttacta ccagaggcat tatataaata tcgacaggag cgcattcgat
     5821 aaatggctcg aatcc
//
LOCUS       DAOSCC010000153         5830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8717_length_5830_cov_4.501126, whole genome shotgun sequence.
ACCESSION   DAOSCC010000153 DAOSCC010000000
VERSION     DAOSCC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8717_length_5830_cov_4.501126"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLG72_08435"
     CDS             <1..514
                     /locus_tag="PLG72_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/uracil permease"
                     /protein_id="HOJ80861.1"
                     /translation="IRPRVNGTVIMGALSLVMLNIGANISFGGITASIAGTTQNFDHL
                     SIINSLADFPSSLFGGPPIEAIISGTAGAPWPVACGIVFMLVTGVLVLTGVIDRLGKY
                     LPSQSIAGFLLVIGFALTFAPNLSAVAGSDDPMSGYIALGVTAWSKNPFLGMVVGIIV
                     RYLGAYVGLV"
     gene            546..1130
                     /locus_tag="PLG72_08440"
     CDS             546..1130
                     /locus_tag="PLG72_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ80862.1"
                     /translation="MLNQKWPSAWKVRIDEDLEIELPLVEIGNDFYIYSFDMTGESKW
                     NKHAARALAKKLAGCSYDGFVTVQTKSSGLCQEMARDMDCYLELRKSHKSFMKDPKHV
                     LVESITTQGKQELWIGREKYERFQGKKLCFVDDVVSTGGTVDAALTMAKEIGFEISVI
                     ACVLTEGEERTNYKGIPLISLGHIPLPGRLADNE"
     gene            1559..2632
                     /locus_tag="PLG72_08445"
     CDS             1559..2632
                     /locus_tag="PLG72_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOJ80863.1"
                     /translation="MADVKPLPPDAELETKRVLKQLTRSHRALAELKGFASTIPNKNI
                     LINAVTINEAKDSSEIENIVTTHDELFKAMSQDNYNNPAAKEVVNYRTALWHGYELIR
                     KKQILTTNMIIEIQQLIEKNRAGIRKLPGTVLKNEATGEVIYTPPAGEKEIMGLMDNL
                     EKYINDDYDNVDPLIKLAVIHYQFESIHPFYDGNGRTGRIINVLYLVLKDLLDSPILY
                     LSKYIICNKSAYYRLLQEVRTEGNWEEWILFILEGIEHTAEETLLLIKRIYDILEKTA
                     DEIKSQLPKIYSRELVNLLFYEFYTKIAYIENGLDITRKTASNYLSSLEEKGFLISQK
                     VGKERIFLNKRLFEVVRQAGTER"
     gene            2716..2898
                     /locus_tag="PLG72_08450"
     CDS             2716..2898
                     /locus_tag="PLG72_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ80864.1"
                     /translation="MATARLEQVSIPENKRIICISDVHGKTDLLRKLLDKVKFSDQDI
                     LILVGDLCVGRHDKSQ"
     gene            2921..3745
                     /locus_tag="PLG72_08455"
     CDS             2921..3745
                     /locus_tag="PLG72_08455"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ80865.1"
                     /translation="MGLRVGLLGVGPVGENIVRVLRERKFPVDGELVVMATSERKETL
                     AGETYDVRKIDESIFKKLDLVFFAGKEGAKGASVQWGDIAVKSGCIVIDNGSDFRMHP
                     NYPLVVPEINMDVVTKDTRYICNPNCSTIQMVVALAPLHRAARIYRITVSTYQAVSGH
                     GGAAMAELKKETSQALNGENVEHDRSIFARPIAFNCIPHIDRFLDNGYTREEMKMINE
                     TRKIFNEPNLLINPTTVRVPVLNGHCESINAEFMLPMDREKAIQILSDKSQSPGVC"
     gene            3991..4749
                     /locus_tag="PLG72_08460"
     CDS             3991..4749
                     /locus_tag="PLG72_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ80866.1"
                     /translation="MDHLLQAMDLNWTQWITVIVVALIIGFSKTAISGAAMFAIPVLA
                     SVFGGKDSTGIMLPMLLVGDLFAVWYYRKSVEWKKVFLPLPWALVGIILGGFVGNRIS
                     DRTFLTLIGIIILCCLGILVYTEVKGKSFKVPDGAWFYIPVGVLSGFASMIGNAAGPI
                     FVIYLLALGFAKNNFMGTNAWFFFIINLIKLPLQIFFWHNIGARSVAVTAAMLPLIAL
                     GAVVGYYILKKINEKVFRYIVIIMTAIAAIKLFV"
     gene            complement(4871..>5830)
                     /locus_tag="PLG72_08465"
     CDS             complement(4871..>5830)
                     /locus_tag="PLG72_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80867.1"
                     /translation="RFAGAVTFPYENDRSMLVSIIRGKSPGERIKRAHDICSRILRKK
                     LGISISCGISAEAHSPGMLQNLFPDAAAALDARFWKGPGTAAFHSPGCLSEDPNLSLD
                     QDTLNKIILNIKLSNLQTALSFIESVSDSLKASPAPNYKPALVREFFWQFIQSVITIL
                     NIQLPFIHQESVVSGRHPYDRIREIRFCSDLEDYVKELLRRIYDFYHDKNPVDNANII
                     ENAKRVIENNFAGDISLEQVARHVHLSPAYLSELFKKQTGMSFIDYKTIVRIENAKKL
                     LSGPSVTISEVSAKVGYSDPKYFSKLFKKITGKTVYEFKKGHS"
BASE COUNT         1592 a         1233 c         1510 g         1495 t
ORIGIN      
        1 cataaggccc agggtgaatg gtacggtgat catgggggct ttgagcctgg tcatgctgaa
       61 tatcggcgcg aacatctctt ttggcggcat tactgcaagc attgccggta ccacacagaa
      121 ttttgaccat ctttccataa taaattccct ggctgatttt ccaagctcgc tgttcggagg
      181 accgccgatc gaagcaataa tatcgggtac tgcaggcgct ccgtggcccg ttgcctgcgg
      241 catagttttc atgcttgtta caggcgtcct ggtattgaca ggcgtgatcg accggctcgg
      301 caagtatctg cccagccaga gcattgccgg tttcctgctt gtgatcggct ttgcactcac
      361 attcgctcct aatctgagcg cagtggcggg atccgacgat ccgatgagcg gttatatcgc
      421 gcttggcgtg acggcatggt ccaagaatcc tttccttggc atggtggttg gtattattgt
      481 gcgttacctc ggtgcgtacg taggtttggt atagggaata cgcagtgtca ggaggaaata
      541 cagacatgct taatcaaaaa tggccttccg cctggaaggt gcgtatagat gaagatcttg
      601 aaatagaact gcccctggtg gagatcggca atgatttcta tatatattcc tttgatatga
      661 ccggcgaatc aaagtggaac aagcatgcgg caagggcgct ggccaaaaag cttgcaggct
      721 gcagctatga cggttttgtg acggtgcaga ccaaatcctc gggcctttgc caggagatgg
      781 ccagggatat ggactgctat cttgaactgc gcaaatccca caagtccttt atgaaggatc
      841 cgaagcacgt actggtcgaa tccatcacaa cgcagggcaa acaggagctg tggataggca
      901 gggagaagta cgaacggttc cagggcaaaa agctctgctt cgtggacgat gtggtgtcga
      961 ccggcggtac ggtagatgca gcgctgacca tggctaaaga gatcggtttt gagatctcgg
     1021 ttattgcctg cgtcctgaca gaaggagagg agcgtaccaa ttataaaggg atacccctga
     1081 tttcactggg acatatccct ctgcccggca ggctcgccga caacgaataa gccggtgaaa
     1141 taagcaaaca catcaacaga caagtaaagc cggataatgg gcagacccgg cggtccagga
     1201 cgcggacctg gcgggtccgg cggaccgggc ggattctggc gcggacccgg cagattcaga
     1261 cacagaccga gaggatttgg gcctcctccg ccgccaagaa gaagattttg gtttcctccg
     1321 ccaccaagaa gatattggcg ccccgcaggc tgccttgggt gcggcgggtc catctttgcg
     1381 gttcttgcag cggttggatg tactatagcg gttatgataa tgcttcttta gacttgcttc
     1441 atgtgtaaaa aatactcacg tcataaaaac gtgggtaatt ttttttattt tttacccatg
     1501 tgcttattga aactgggaat aattacacat ataatgttga tgtaaggagt gaatttaaat
     1561 ggctgatgta aagcctttac cgccggatgc agaacttgag accaaaagag tattgaagca
     1621 gcttacgaga tcccatagag ctctggcaga actcaaaggt tttgcgagta cgataccaaa
     1681 taaaaatata cttataaatg ctgtaactat caatgaagcc aaagacagtt ccgaaatcga
     1741 aaatattgta actacgcatg acgaactgtt taaagcaatg tcccaggaca attataataa
     1801 cccggcagcc aaagaagtcg taaactacag gactgcactt tggcacggtt atgaacttat
     1861 caggaaaaag cagatcctga ccaccaacat gatcattgaa atacagcaac tgattgaaaa
     1921 aaaccgcgca ggcataagaa aacttccagg tactgtactg aaaaacgaag cgaccgggga
     1981 agtgatatat actccgccgg ccggagaaaa agaaataatg ggattaatgg ataacctcga
     2041 aaagtacata aatgacgatt atgacaatgt cgatcctctc attaagctgg ctgtcatcca
     2101 ttaccagttt gagtccatac atccgtttta tgatggcaat ggaaggacgg gcagaatcat
     2161 aaatgtactg tatcttgtcc tgaaagattt gctggacagt ccgatactct atctcagtaa
     2221 atatataata tgcaataaat cagcatatta caggctcctt caggaggtaa ggaccgaagg
     2281 caactgggag gagtggatat tatttattct tgagggtata gagcatactg ccgaagaaac
     2341 attactgctg ataaagagaa tatatgatat tcttgaaaag acagccgatg aaattaaaag
     2401 ccaactgccc aaaatatatt cgagagaact tgtaaattta cttttttatg agttctacac
     2461 caaaatcgca tatatagaga acggactcga catcacgagg aaaactgcct cgaattactt
     2521 atcatctttg gaagaaaagg gattcctgat atcgcaaaaa gtcggcaaag aaagaatctt
     2581 tcttaacaaa cgcctgtttg aagttgtgcg gcaggccggt acggaaaggt agacttacgc
     2641 aattttactg tatcatactg tgataacagg taatctgctg caaaaagata aggacacggg
     2701 aggaaagggc ttgagatggc gactgccagg ctggaacagg tttcgatccc tgaaaacaaa
     2761 cggataatct gtataagcga cgtgcatgga aagaccgact tactcaggaa acttctggac
     2821 aaggtaaaat tttctgatca ggatattttg atactggttg gggacctttg cgtcggccgg
     2881 cacgataaaa gccagtaaca aaaatgaaag agggatcaaa atgggactca gagttggttt
     2941 gttaggggtc gggccagtag gagaaaatat cgtacgggtt ctccgtgaac ggaaatttcc
     3001 cgttgacggc gaactggtcg taatggcaac atcggaaaga aaagagactc tggccggaga
     3061 aacatacgat gtaaggaaga tcgatgaaag catcttcaaa aaactcgatt tggtattctt
     3121 tgcaggaaag gaaggagcca aaggggcaag tgtacagtgg ggagatatag ccgtcaagag
     3181 cggatgtatc gttatcgaca atggcagcga ttttcgtatg catcccaatt accctttggt
     3241 cgttcctgaa ataaacatgg atgtcgttac aaaggacacc cggtatattt gcaatccgaa
     3301 ctgctcaacg atccagatgg ttgtggctct cgctccgctc catcgagctg ccaggatata
     3361 caggataact gtttccacct accaggctgt aagcggacat ggcggcgctg ccatggctga
     3421 actgaagaaa gagacttctc aggctttgaa cggggagaat gtggagcatg acaggtcaat
     3481 attcgccagg cccatagctt tcaactgtat tccgcacatt gacaggtttt tggataacgg
     3541 atataccagg gaagaaatga agatgataaa tgaaaccagg aaaattttca atgaacccaa
     3601 cttgctgata aaccctacga ctgtgcgagt gccagtgctg aacggccact gtgagtcgat
     3661 caatgcagag ttcatgctgc cgatggatag ggaaaaggct attcaaatac ttagtgataa
     3721 gagccagtcc ccgggcgtat gctgaagctg gggtacctgt aaggaagatg caaggcgctg
     3781 cagcaggcaa atccatagtt tatatatacc atactcattt tttgacaccg atgagtatcc
     3841 aaagtccatt gaagtcgttg tagaatggaa ttagtccttt gcaatcctgt gccatgcctg
     3901 taatgaaata acctggtgag gatatgaaat aataacggcc ataggacaga agtaccgatc
     3961 agccggagtc aacctgagag gagacacaac atggatcatt tgctgcaagc aatggattta
     4021 aactggacgc aatggatcac tgtaatcgta gtggcattga taataggctt ttcaaaaacg
     4081 gccatcagcg gggctgcgat gtttgcaata cctgttcttg catctgtatt cggcggcaaa
     4141 gattctacag gtatcatgct gcctatgttg ttagttggtg atttatttgc ggtttggtat
     4201 tatcgtaaaa gcgtggaatg gaaaaaggta tttcttcctt tgccctgggc gctggtcggc
     4261 ataatcctcg gaggttttgt agggaatcgt atcagtgaca ggaccttcct gacattgatc
     4321 gggatcatca ttttatgctg ccttgggatt ttggtttaca ctgaggtaaa aggcaaaagc
     4381 tttaaggtac cggacggggc ctggttttac attcccgtag gagtgctgag cggctttgcg
     4441 tcaatgatcg ggaatgcagc aggtcccata tttgttatct acctgttagc attaggtttt
     4501 gcgaaaaata actttatggg gacgaatgcc tggtttttct ttataatcaa tctcataaaa
     4561 ctgcctctgc aaatcttttt ctggcataat atcggcgcaa ggtccgtggc tgtcaccgca
     4621 gcaatgctgc ctttgattgc tctgggtgcc gtcgtgggtt actacatact gaaaaagatc
     4681 aacgaaaagg tttttcggta tatagttatt ataatgacag ctatagctgc cataaaactt
     4741 tttgtttagt tattgcggaa ctctgcttgt cagtggtaaa aggacactcc ggagcaagga
     4801 aaggggacac acctctacag gaggcattcc tttatggaag aggtatgtcc ccttatgcaa
     4861 agttcaaggc tcaggaatgt ccctttttga attcgtacac tgttttgccg gtgattttct
     4921 tgaacagctt gctgaagtac tttgggtcgg agtagccgac cttcgcgctt acttcgctga
     4981 tggtgacgga aggacccgag agcagctttt tcgcattctc gatcctcact atagtcttgt
     5041 aatctataaa gctcatccct gtctgcttct tgaagagttc gctcagataa gcagggctga
     5101 ggtgcacgtg ccttgccacc tgctcaaggc ttatgtcgcc tgcgaaattg ttttctatga
     5161 ctcttttggc gttttcgatg atattcgcgt tgtcgacggg gttcttgtcg tggtagaagt
     5221 cgtagatgcg cctgagcagt tcttttacat agtcctcaag atcggaacag aaacgtatct
     5281 ccctgatcct gtcatacgga tgccggcccg ataccacgct ttcctggtgg atgaaaggca
     5341 gttgtatgtt aagtatcgtg atgacggact ggatgaattg ccagaaaaat tcccttacca
     5401 gggccggttt gtagtttggc gcaggcgaag ccttcagcga gtctgaaaca ctttcgatga
     5461 atgacagggc ggtctggagg tttgacagtt ttatattgag gattatcttg ttgagcgtgt
     5521 cctgatccag cgaaagattc ggatcttcag acaggcagcc gggcgaatgg aatgcggcgg
     5581 tacccggccc cttccagaat cttgcgtcaa gagccgccgc agcgtcgggg aacaggttct
     5641 gcagcatgcc gggcgaatgg gcttcggcgc tgatgccgca tgagatgctg atcccaagtt
     5701 ttttccgcaa tatcctgctg cagatgtcat gggctctttt tatccgctct cccgggcttt
     5761 ttcccctgat gatcgaaacg agcatggacc tgtcgttttc ataggggaac gtcacggcgc
     5821 cggcgaatct
//
LOCUS       DAOSCC010000154         5803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8775_length_5803_cov_4.642310, whole genome shotgun sequence.
ACCESSION   DAOSCC010000154 DAOSCC010000000
VERSION     DAOSCC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8775_length_5803_cov_4.642310"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..186
                     /locus_tag="PLG72_08470"
     tRNA            112..186
                     /locus_tag="PLG72_08470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:145..147,aa:Arg,seq:ccg)
     gene            307..1278
                     /gene="dusB"
                     /locus_tag="PLG72_08475"
     CDS             307..1278
                     /gene="dusB"
                     /locus_tag="PLG72_08475"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOJ80868.1"
                     /translation="MKIGSLTLENNIFLAPMAGVTDMPFRILCKEQGCGFVYTEMISA
                     KGMYYNDEKTWKLAELSQAELPAAIQIFGSEPDIMAHAAEKLNGSPAAVIDINMGCPT
                     PKITKNGDGSALMLKPELAGEIIRAVASASSKPVTVKIRKGWDDGHVNAVEFAVMAEK
                     CGAAAVTVHGRTREQFYSGQADWDIIRDVKRAVSIPVIGNGDIRSPEDAARMLEYTGC
                     DAVMVGRGAQGNPWLFKRLGYFLKTGQMLPEPDIEEKKAMMLRHLDMMVEYKGEYTGV
                     REMRKHIAWYIKGCKNSSRFREKAFKASAYKEMAELIKTFGIENAGQ"
     gene            1521..3794
                     /locus_tag="PLG72_08480"
     CDS             1521..3794
                     /locus_tag="PLG72_08480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ80869.1"
                     /translation="MNLEQMLERIRSSQDMMKNITAWVEVPAKEAVYADFPDFLDERI
                     VKALERRGVRKLYSHQASAIQAVHDGDSIVVVTPTASGKTMCYNIPVMDAILKDESSR
                     ALFIFPTKALSQDQTAELHELITEAGVDVKTYTYDGDTPQSARKAIRQAGHIVVTNPD
                     MLHSGILPHHTKWTKLFENLKYVVIDEVHHYRGVFGSHLANVMRRLRRICDFYGSKPQ
                     FICCSATIANPVELASKIVGREVRLIDNNGAPSAAKHIIFYNPPVINKELGIRKSSML
                     EAERIAESFIRNGIQTILFTRSRLNVEVLVTYLKEIYKGDREAEKKVRGYRGGYLPTL
                     RREIEKGLRDGTITGVVSTNALELGIDIGALEACIICGYPGTIASTWQQAGRAGRRSG
                     VSAAILVASSSPLDQYIINNPDYFFGRTPEYGLINPDNLVILYSHMKCAAFELPFEDG
                     EKFGVETTAEILGFMEEAKIVRHVGNRWHWMADVFPADEISLRSASNENFIIIDITEP
                     KPVVIGEVDRFSAPMMLHEEAIYIHEGVQYQVEKLDFDDKKAYIRKVNVDYYTDANLA
                     VDLKVLDVFREDKSKQVHRSVGEVSVTALVTMFKKIKLHTHENIGSGPVNLPEMEMHT
                     TSYWVSLPETIPGMSQTELQNGLLGLSNVLANAAPIYLMCSPGDIQVVHQVRATFTQR
                     PSVYIYDNYPGGVGFSDKLYELHGQLFETAAQMVEQCGCESGCPSCVGPLDQFTGTDD
                     PKGLTLRLIKLIRQESQ"
     gene            3913..5286
                     /locus_tag="PLG72_08485"
     CDS             3913..5286
                     /locus_tag="PLG72_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOJ80870.1"
                     /translation="MTGAVVLRKEAQYPGGNGRIGFENRSNIRMDLRSKLNLYRQTQA
                     ASSQAPKTGVGHNADAAGRGRDIDVLIPGTVRENEHGSCYIIEESYHPGCIHGGCRIG
                     AASGISGETLAVLGGPDCDGLQADKLLYLDTETTGLAGGTGTVAFLVGTGFFENGSFI
                     IRQYFMRDYDEEAAMLAELKELISGRQGLVTFNGKCFDINILQSRFISNRFRTDISGM
                     PNIDLLFPARRVWGMKLESCRLASLEENILGHARHDDIPGSLIPAVYFKYLEDRDASE
                     IKRVIEHNKLDVLSMVSLLYRLSAMLQDPLAESDGGLELLGIGRIFETIGRTEDMVEC
                     LEACTGSGRFDVKLQAVKRLTGVYKREGRYDRALEHWQAIESEGSGFELFHLIEMAKY
                     YEHRAKDPEKALQAVEKAFAVCRQAGLTAGRQIDELAKRRDRLRRKINIMETSKGQCC
                     RRDIENL"
     gene            complement(5447..>5803)
                     /locus_tag="PLG72_08490"
     CDS             complement(5447..>5803)
                     /locus_tag="PLG72_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80871.1"
                     /translation="EEAIEEIKKCAGKQFDPFLAGKFIKIIEKGNGSGTGGTATDVIS
                     ETGDMDGDPGETDGYGAAEAVGDGLGETVADGIIRPDNSSETDGNVVTDGTDTDSTKS
                     RAPSAASASAASAVPG"
BASE COUNT         1546 a         1434 c         1644 g         1179 t
ORIGIN      
        1 aatttgttct tttttctaaa tgacaaaaaa gatctggcaa acatgagctt cactgcatca
       61 atactgttgt caaaatgctt aaaataggct ataatactat agcagataag tgtcccggtg
      121 gtctaatgga taaggtaatg gattccggtt ccattgatgg gggttcgatt cctctccggg
      181 acgccatgtt aagcgggtct tggcgagtat tcccaaggtc cgcttttata tattgccggc
      241 atcgacacgg accgctgcgg atggatgacg gcagtgaaat acgtattgcg gcaggggaag
      301 caacggatga aaattggttc gctgacattg gaaaacaata tatttcttgc gcctatggcg
      361 ggcgtcaccg acatgccctt caggatcctc tgcaaagagc agggctgcgg tttcgtatat
      421 acagagatga tcagcgccaa aggaatgtac tataatgacg aaaaaacctg gaagctggct
      481 gaactcagcc aggctgagct tccggcggcc atacagattt ttggctccga accggatata
      541 atggcgcatg ccgctgagaa actgaacggc tctccggcgg ctgtcataga cataaacatg
      601 ggatgtccca cgccgaagat cacgaaaaac ggtgacggaa gcgcacttat gctcaaaccg
      661 gaacttgccg gcgagataat acgggccgtg gcgtcggcat cctcgaagcc tgttacggtg
      721 aaaatccgca aaggatggga cgacgggcat gtaaatgccg tagaattcgc cgttatggct
      781 gagaaatgcg gagcggccgc tgtgaccgtg catggccgta cacgggaaca gttctacagc
      841 gggcaggctg actgggatat catacgggat gtcaaaagag cggtttccat acctgtcatc
      901 gggaacggcg atatccgttc cccggaagat gcggcccgca tgctggaata cacgggctgt
      961 gacgccgtaa tggtaggcag gggcgcccag ggaaacccgt ggttattcaa aaggctcggc
     1021 tacttcctca aaacaggaca aatgcttccc gagcccgata tagaagagaa aaaggcgatg
     1081 atgctgcgcc acctcgacat gatggtagaa tacaagggcg agtatacagg cgtacgggaa
     1141 atgcgcaagc acattgcctg gtacatcaag ggatgcaaaa actccagccg tttcagggaa
     1201 aaggctttca aggcgtcggc atataaagag atggcagaac tgataaaaac tttcggaatc
     1261 gaaaatgcgg gccaatgatc ctgcctgtca cacacatacc gccgcaggca tggatttcat
     1321 gggcgatgcg caatttgcgg atattatcgc agcaagaatg cgggaaagtt tgaggcgagg
     1381 ctgtaaacgg gcctgaaccg gccatgctga cgcagcatcc ggtttatcct gtcgcggcaa
     1441 gccggagcaa tccaacactt cctttacatc gtttatgttt tgcattaaaa tatttacaga
     1501 atatatgttc ggaggatgcc atgaaccttg agcaaatgct ggagcgtatc agatcgtcgc
     1561 aggatatgat gaagaacatc acggcctggg tggaagtacc ggccaaagag gctgtatacg
     1621 ccgatttccc ggatttcctg gatgagcgca tcgtaaaagc cctcgaaagg agaggggtca
     1681 ggaagctcta ctcgcaccag gccagcgcga tccaggcggt acacgacggg gacagcatcg
     1741 ttgtggtgac gcccactgca tcaggaaaga ccatgtgcta caacataccg gtcatggacg
     1801 ccatattgaa ggacgagtcc tccagggcat tgttcatttt ccccacaaaa gcgctgtcgc
     1861 aggatcagac cgcggaactc cacgaactga tcacagaggc aggagtggac gtcaagacat
     1921 acacttatga cggcgacacg ccccaatcgg caaggaaagc cataaggcag gcgggccata
     1981 tagtggtcac caatcccgac atgctccaca gcggcatact cccgcaccat acgaaatgga
     2041 ccaagctctt cgaaaacctg aagtacgtcg tcatagatga agtgcaccat tacaggggcg
     2101 tattcggcag ccacctggcc aacgtcatga gaaggctcag gaggatatgc gatttctacg
     2161 gttcaaaacc gcagttcata tgctgctccg cgaccatcgc caaccccgtg gaactggctt
     2221 cgaaaatagt cggccgggaa gtcaggctca tcgacaacaa cggcgcgccg tctgccgcca
     2281 agcatatcat tttttacaat ccgccggtca tcaacaagga gctgggcatc aggaaaagca
     2341 gcatgctgga ggccgagcgc attgcggagt cgttcatacg gaacggcata cagaccatat
     2401 tgttcacaag gagccggctg aatgtagagg tgcttgttac ctacctgaaa gagatataca
     2461 agggggacag ggaagccgag aagaaggtgc gcggttacag gggaggttat ttgcctacgc
     2521 tccggcgtga aatagagaaa ggcctgcggg atggcaccat aacaggagtc gtatctacga
     2581 acgcgctgga actgggcatc gacatcggcg cgttggaagc atgcattatc tgcggttatc
     2641 cgggaaccat cgccagcaca tggcagcagg ctggccgcgc agggcgcagg agcggcgtat
     2701 ccgccgctat cctcgtggca agcagcagcc cgctcgacca gtatataatc aacaacccgg
     2761 actacttctt cggaaggacc ccggaatacg ggcttataaa ccccgacaac ctggtcattt
     2821 tgtacagcca tatgaagtgc gccgcatttg aactgccctt cgaagacggt gagaaattcg
     2881 gagtggaaac tacagcggag atccttggat tcatggagga ggcgaagatc gtaaggcacg
     2941 tggggaaccg gtggcactgg atggccgatg tgttcccggc cgatgagata agcctgagaa
     3001 gtgcatccaa cgaaaacttc atcataatcg acataacgga accgaagcct gttgtcatag
     3061 gggaggttga caggttcagc gcgcccatga tgcttcacga ggaagcgata tatatacatg
     3121 aaggcgtcca gtaccaggtg gagaagctgg atttcgacga caagaaggcc tatatccgaa
     3181 aggtcaacgt ggactattac actgacgcca acctggccgt ggacctcaag gtcctggacg
     3241 tattccgcga ggataaatcg aaacaggtgc acaggagcgt cggcgaagtt tcggtcaccg
     3301 cgctggtcac catgttcaag aagataaagc tccataccca cgaaaatatc gggtcaggcc
     3361 cggtaaacct gccggaaatg gagatgcaca ccacatccta ctgggtcagc ctgcctgaaa
     3421 ccatacccgg catgtcccag acagaactgc agaacggtct gctcgggctt tccaacgtgc
     3481 ttgcaaatgc ggcgccgatc tatctcatgt gcagccccgg agacatccag gtcgtacacc
     3541 aggtacgggc cacattcacg caaaggcctt cggtttacat atacgacaac tatccgggag
     3601 gagtaggctt cagcgacaag ctgtatgaac tgcacggcca actgtttgag actgccgccc
     3661 agatggtgga gcaatgcggc tgtgaatcgg gctgcccttc atgcgtcggg cccctcgacc
     3721 agtttacggg gaccgacgac cccaaggggc tgacgctcag gctgattaaa ctgatcaggc
     3781 aggagtcgca gtagtaaaac gggaaccatg gtatccgggt atggcgttat tcaggcagga
     3841 atttcggtat tcgactggaa caatagaatc agggctagaa cgtagtattg cggcaagagc
     3901 cgtagcatta ttatgacagg agccgtagta ttaaggaagg aagcgcagta tccgggcggg
     3961 aacggcagga ttggctttga gaacaggagc aatattcgga tggatctcag gagcaagctg
     4021 aatttatacc ggcaaacaca ggcagcatca tcgcaggcgc caaaaacggg cgttggtcac
     4081 aatgcagatg ccgcgggaag agggcgcgac attgacgttc tgatacccgg tacggtacgg
     4141 gagaatgagc atggctcctg ctatatcatt gaagaatcat atcatcccgg atgcatacat
     4201 ggcggctgca ggatcggcgc tgcatccgga ataagcggtg aaacgcttgc agtacttggc
     4261 ggtcccgatt gtgacggcct tcaggccgat aagctgctgt atctcgatac ggaaaccaca
     4321 gggctggccg gagggacggg gaccgtcgca ttcctggtcg ggaccggctt tttcgaaaac
     4381 ggcagtttca taataagaca gtatttcatg cgcgattacg atgaagaagc cgcaatgctg
     4441 gctgaactga aggagttgat ctcgggacgg cagggccttg ttacgttcaa cgggaaatgc
     4501 ttcgatatca acatccttca aagcaggttc atctccaaca ggttcaggac cgatatctcc
     4561 gggatgccca atatcgatct gctgttcccg gcaagaaggg tatggggcat gaaactggaa
     4621 agctgccgcc tggcatcgct tgaggaaaat atcctgggcc atgcgaggca tgacgatata
     4681 ccgggatcgc tgataccggc cgtttacttc aagtaccttg aagacaggga cgcatctgaa
     4741 ataaagaggg taatagagca caacaagctt gacgtattgt ccatggtgtc gctgctgtac
     4801 cggctttcag caatgctgca ggatccgctg gccgaatctg acggcgggct tgagctgctg
     4861 ggcataggca ggatcttcga gaccatcggc aggacggaag acatggtgga gtgccttgag
     4921 gcgtgcacag gttcagggcg gttcgatgta aaactgcagg cggtgaagcg ccttaccggc
     4981 gtttataaga gggaaggacg ctatgaccgt gcgctggagc actggcaggc aatcgagtcg
     5041 gaaggctccg gttttgaact gttccacctc atagagatgg caaagtacta tgaacacagg
     5101 gcgaaggatc cggagaaagc gctgcaggca gtcgaaaaag cattcgcagt atgccggcag
     5161 gccgggctta cggccggaag gcagatcgat gaactggcaa agcgcaggga caggttgaga
     5221 aggaagataa atattatgga gacatccaag ggacaatgct gccgcaggga tatagaaaat
     5281 ttataataag catggaagtc gtgcctgcga cgcagatata atataaccac caataaagat
     5341 catgcgcgta cgcaaacaac cttacagatg ctaaaggaaa catgcagaca caaagacgca
     5401 gcagggatca taaaatgcca caactgtcat aagggtaccg cagagattat ccaggtaccg
     5461 cagatgctgc agaagccgaa gccgctgaag gcgcccggct cttggtactg tcagtatcag
     5521 tgccgtccgt cacaacattg ccatccgttt cgctgctgtt atccggtctg attataccat
     5581 cggcgactgt ttcacccaaa ccatcaccaa cagcttcggc agcaccataa ccatccgttt
     5641 cacctggatc gccatccata tcgcctgttt cacttataac gtccgttgca gtgccgccgg
     5701 ttccgctgcc gttacctttt tctattattt ttatgaactt cccggccagg aatgggtcaa
     5761 actgtttgcc tgcgcacttt ttgatctctt ctatcgcttc ttc
//
LOCUS       DAOSCC010000155         5778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8818_length_5778_cov_5.300542, whole genome shotgun sequence.
ACCESSION   DAOSCC010000155 DAOSCC010000000
VERSION     DAOSCC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8818_length_5778_cov_5.300542"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLG72_08495"
     CDS             complement(<1..310)
                     /locus_tag="PLG72_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80872.1"
                     /translation="MNSFRYISDLLHKHRYRYVLGVVSLLCVDTLQLIMPKILEAATD
                     SLEEGVLTRSSLAAYAAALVLVSVGIAVFRFLFRYTLYGVSRSIELSFRNRLYAHLQK
                     L"
     gene            565..1170
                     /locus_tag="PLG72_08500"
     CDS             565..1170
                     /locus_tag="PLG72_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ80873.1"
                     /translation="MGLHNYLMTVRKLNNMGRWSTDFMHQRATVSEHSFFVAQIGQML
                     ALIEEQNGNKVDWSRLFKKLLNHDVVEAMTGDIISTVKHKNAQLRDMLNMLEKEVVEE
                     NLLASMEEPYKSIYREILFDGKDSTLEGEILKCADHIDALVECINEVKLNNLYPFVEK
                     FHAIVKILSGSNLVSVRYFIGKILPGMIEGCKELERSDLIK"
     gene            1352..1804
                     /gene="fabZ"
                     /locus_tag="PLG72_08505"
     CDS             1352..1804
                     /gene="fabZ"
                     /locus_tag="PLG72_08505"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOJ80874.1"
                     /translation="MLTNLDIRKILPHRYPFLLVDKIIELEPGKRAVGIKNVTADEPF
                     FQGHFPDYPIMPGVLIVEALAQTAGIAANAETAQAEGENGETKLGVFAGIDEMKFKKQ
                     VMPGDTLRLEAEILSSKMGIVKAKVRAAVDDKTAAEGEIRFAMTTVKK"
     gene            complement(1906..3267)
                     /gene="murC"
                     /locus_tag="PLG72_08510"
     CDS             complement(1906..3267)
                     /gene="murC"
                     /locus_tag="PLG72_08510"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ80875.1"
                     /translation="MTGIGGVSMSGLARILAYQGYIVTGSDERPSPATQKLEKEGIKV
                     TIGHCAENVNGADLLVYTAAVKPDNVELVAASGAGIPCINRATLLGEIMKKYPMSFAV
                     SGTHGKTTTTSMLSLIMLEEGLDPTIHVGGELAAIGGNIRIGGSEYFVTEADEYTGSF
                     LKFYPHVAVILNIDYDHVDCFRNIDDVKKAFHGFAEHVPENGHIVVNGDDISALDALN
                     GISCDITTFGLRDSHNEWSAANIEFDDMGCASYTLLRVGEPVAEIKLKVPGIHNVSNS
                     LAAIAASSLAGCSISSAVRALHKFTGTCRRFEIKGVSDGVTVVDDYAHHPSEITATLK
                     AAVKYNHSRIWCVFQPHTYSRTKSLMDDFSRAFDNADFVILTDIYAAREKDTGEVNSS
                     MLAEKIALTGKKAVYIKDFGSIVELLDKNVMPGDLVITMGAGDIYKVGDMFLESRKKL
                     AVS"
     gene            3768..4634
                     /gene="purR"
                     /locus_tag="PLG72_08515"
     CDS             3768..4634
                     /gene="purR"
                     /locus_tag="PLG72_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HOJ80876.1"
                     /translation="MKMQVLTKESDDMDKLHRNERISVLQRILCESPGKVFTLGYFTD
                     MLNCAKSSVSEDIDIIRSSLEKTGAGTIETIPGASGGVRYVPMMSEDAVQQLAGRLSA
                     ELSIRERILPGGYLYMLDTIYDPGTVSEVGRVFAGYFRNRTVDYVITVETKGIPLAFM
                     TAVYLNKPLVIARHNIEATDGASVNINYVSGSSDRIQTMVLPMRSLRRGSWLLFIDDF
                     MKGGGTAKGILELAREFGCQVAGAGVLVETAMPDRKLPVDYFSLLTLYGVDEASGVTD
                     IRPSPRLIAGGR"
     gene            4785..5114
                     /gene="spoVG"
                     /locus_tag="PLG72_08520"
     CDS             4785..5114
                     /gene="spoVG"
                     /locus_tag="PLG72_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOJ80877.1"
                     /translation="MEITDVRIRKIDVEGKMKAVVSVTFDNEFVVHDIKVIESQNGMF
                     IAMPSRKTPDGEFKDIAHPINAATREKIQKAILDKYIATTTAEETTASSEGPADQTDG
                     STESSEE"
     gene            5232..>5778
                     /locus_tag="PLG72_08525"
     CDS             5232..>5778
                     /locus_tag="PLG72_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOJ80878.1"
                     /translation="MESITALILAAGEGKRMKSDKAKVLHQVCGKPMIEWVYEAARDA
                     GVGKCVIIVGHKAEQVMEHMGDRAEYVMQEQRLGTGHAVQQARSYMEDEGYILILYGD
                     TPLITSETIKKAMDCMLDEKLDAVVISADVEDPSGYGRIVRNPDGSFKRIVEHRDASP
                     AEREIKEINSGMYIFKARELAD"
BASE COUNT         1584 a         1290 c         1492 g         1412 t
ORIGIN      
        1 aaagcttctg aaggtgtgcg tatagcctgt tcctgaagga aagctctatg gatctcgaca
       61 caccgtaaag agtatacctg aaaaggaaac ggaataccgc tattccgact gataccaata
      121 ccagcgcggc agcgtaggct gccaggctgc ttcgcgtcaa gactccctct tccagcgagt
      181 cagtggcggc ttccagtatt tttggcatga taagctgcag tgtatctacg caaagcaggg
      241 aaaccacgcc caaaacgtat ctgtacctgt gtttgtgcag caaatcgctt atgtacctga
      301 aactgttcat gcaatacgct cctgaaagtc acaactgtac ggtactgatt atatcacatt
      361 ggctgtttcc aaagacaata aacatgtacg gctatgtttg aaatctttag tctgatataa
      421 taagatataa taaaacataa ggcagtacat gcgggcaaaa cagccttttt accggcggta
      481 tgcctgcagg gacgatccgg tgttatacag cttcccggcc tggagcacgg gaaataaaat
      541 cgtgctgtta ttatggagga tgccatggga ctgcacaatt acctgatgac tgtgcgaaag
      601 ctgaacaaca tgggccgctg gtccacagac tttatgcatc agagggctac tgtcagcgag
      661 cattcctttt ttgttgcaca gattgggcag atgcttgcgc tgatcgaaga acagaacggc
      721 aataaggtgg actggagcag gctgtttaag aaactgctga accatgatgt agtggaggca
      781 atgaccggcg acatcataag caccgtcaag cataaaaatg cgcagctgag ggacatgctg
      841 aacatgcttg agaaggaggt cgtcgaggaa aacctgcttg ccagcatgga ggaaccatat
      901 aagagcattt acagggagat acttttcgac gggaaggaca gcaccctgga aggagagatc
      961 ctcaagtgcg ccgaccatat cgacgcattg gtggaatgca tcaatgaagt aaagctcaac
     1021 aacctgtatc cgtttgttga gaagttccat gcaatagtga aaatactgtc cggatccaac
     1081 cttgtaagcg tgcggtattt tatcgggaag atactgcctg gaatgatcga aggctgcaag
     1141 gaactggagc gcagcgatct tataaagtga aacgccgaag cagaaagctt ccgatgtgaa
     1201 aaaccgggcg cagagacccg gaggggcgcc ggccagggtt tgtcggcatc cggagacggc
     1261 ctttataaaa ataccgtccg gatcgacttg taccggacca caaaaaatcg gtttggatac
     1321 aaaaattact atactgaaat gaggtgtaca tatgctgacg aatcttgata tcaggaaaat
     1381 attaccgcac agatatcctt ttttgcttgt ggacaaaatc atagagcttg aacccgggaa
     1441 gagggcggtc gggatcaaaa atgtgacagc cgacgaaccg tttttccagg gacatttccc
     1501 ggattatccg atcatgcccg gagtgctgat agtggaagca cttgcacaga cagcggggat
     1561 cgctgcgaat gccgaaaccg cgcaggcgga aggagagaac ggcgaaacaa agctgggagt
     1621 gtttgccggc atcgatgaga tgaaattcaa aaagcaggtc atgccaggtg atacattgcg
     1681 cctggaagcc gagatccttt catcaaagat gggcatcgta aaggcaaagg tcagggcagc
     1741 tgtggacgac aagacggcgg ccgagggaga gatccgcttc gctatgacaa ctgttaagaa
     1801 ataaaagact tccccgcaag ggaagtcctt tacagcatct ttcggtttat cggtaggccg
     1861 caaatatgcc ggaacaattg atcgataacg atccagcgca aatcatcagc ttactgcaag
     1921 cttctttctc gactcgagga acatgtcgcc gaccttgtat atgtctccgg cgcccatggt
     1981 aatgacaaga tcgccaggca tcacgttttt gtcgaggagt tccacgatgc tgccgaaatc
     2041 cttgatatag acagccttct tgcctgtaag ggcaattttt tcagcaagca tcgacgagtt
     2101 cacttctccg gtgtcttttt cacgcgccgc atatatgtcg gtcaggatga cgaaatctgc
     2161 attgtcgaat gctctgctga agtcatccat aagacttttc gtcctggaat atgtatgggg
     2221 ctggaatacg caccatatac gcgaatggtt gtatttgacc gcagccttca atgtagctgt
     2281 gatttcggac ggatgatgtg catagtcgtc cacgactgtc actccgtcgc tgactccttt
     2341 tatttcaaat ctcctgcatg taccggtgaa tttgtgcaat gccctgacgg cggatgaaat
     2401 actgcatcct gccaggctgc tcgctgcgat cgctgctagc gagttgctga cattatgtat
     2461 tccgggtacc ttcagcttta tctcggcgac cggctcgccc acgcggagca gggtatacga
     2521 tgcgcatccc atatcgtcaa attctatatt tgcagcgctc cattcattgt ggctgtccct
     2581 taacccgaat gtagttatat cgcatgaaat gccgttcagc gcgtcgagag ccgatatgtc
     2641 gtcaccgttt acgactatgt gcccgttttc ggggacatgt tctgcaaagc cgtggaacgc
     2701 tttttttacg tcatctatgt tcctgaagca atccacatga tcataatcga tattcaggat
     2761 gacggcaaca tgggggtaga atttaagaaa acttcccgta tattcgtcgg cttcggtcac
     2821 gaaatattcg ctgccgccga tcctgatatt tccgcctatg gcggcaagtt cgcctccgac
     2881 atggatggtc ggatcgagcc cttcttccaa cataatcaac gatagcatgg atgtggtcgt
     2941 ggtttttccg tgagttcctg aaaccgcgaa actcatggga tatttcttca tgatctcgcc
     3001 caggagtgtg gctctgttga tgcacgggat ccctgctccg gatgcggcaa caagttctac
     3061 attgtcgggc ttgactgccg cagtgtatac cagcagatca gcgccgttca cattttctgc
     3121 acaatggcct attgttacct ttataccttc cttttcgagc ttctgagtgg caggggacgg
     3181 cctttcgtcc gacccggtaa ctatgtatcc ctggtatgca agtatcctgg caagcccgct
     3241 catgcttacg ccgccgatcc ctgtcatgtg cactctcttt atattgccgg aacttaaaag
     3301 ctcgtcagtt ttcaaaccca acactccctg atcatgaaat tcagctaagt aaatgataca
     3361 ataaatgccc gtaaaagtaa aatagatata tctaagcatt gacagctatc tcatataata
     3421 tgccgttttt cacgtcttta gtgcatgatc tccacatcat attttattct tttttcgaat
     3481 tttttatttc agctgctttc cgtattatcg ggctaagcct gttatgaagt ccggcgctga
     3541 cgcctgtacg aactctgata aaactgtgat ataatgatgt aacagggcga cggaagtatc
     3601 tcaattgtat aaatatccgc tgcggatgtt ccattgagac ggtggataca attggacaat
     3661 gagtatattc aggacaatga atatatttac cgcctcgttg aaacactgct gttgtgataa
     3721 aattcttctg aaagcgtgaa attttttgga tttgagtgct gtaaaacatg aaaatgcagg
     3781 ttctcacgaa agagagtgat gatatggaca agctgcacag gaatgagagg atatcagtgc
     3841 ttcagaggat actgtgcgaa tcgcctggta aagtcttcac actgggctat tttacggaca
     3901 tgctcaattg tgcgaaatcc tcagtaagtg aggatatcga tattatacgc agttcgctgg
     3961 aaaagacggg agccggcacc atcgaaacga tacctggcgc atcaggcggc gtgcgctatg
     4021 taccgatgat gtcggaggat gcggtacagc agcttgccgg caggctgagc gcggaacttt
     4081 ccataaggga aaggatactg cccggaggct acctttacat gcttgacact atatacgatc
     4141 ccgggacagt ttccgaggtg ggcagggttt tcgccggcta tttccgcaac aggactgtcg
     4201 attatgtcat cacggtagag acaaaaggca tacctcttgc ttttatgacg gcggtatacc
     4261 taaataagcc gcttgtgata gcgcgtcaca acattgaagc gacagacgga gcgtcagtaa
     4321 acataaacta tgtttccggt tcatcagaca ggatacagac catggtactg ccgatgcggt
     4381 cattgaggcg cggttcgtgg ctgcttttca tcgacgactt catgaagggc ggcgggactg
     4441 cgaaaggcat cctggagctt gcgagggaat tcggctgcca ggttgccggc gcaggcgttc
     4501 ttgtagagac agccatgccc gacaggaagc ttccggtcga ttatttttca ctcctgacac
     4561 tttacggtgt cgacgaagcc tccggagtaa ccgatataag gcccagtccg cgccttattg
     4621 ccgggggaag gtgaaaaacg gaccgtgggg acatatgtcg caatgtgaca acagaccaat
     4681 attataatag ccaaaaataa caaatgaaag gcaaataaga aggaaaatat cattttgtat
     4741 agaatatata agttgcacat tattttggga aggtggtaac ataaatggaa atcacggatg
     4801 tccgcatcag gaaaatcgac gttgagggca agatgaaggc ggtcgtatcg gtaacttttg
     4861 ataacgagtt cgtggtacac gatatcaaag ttatagagag ccagaacggc atgttcatag
     4921 ctatgcccag cagaaagact cccgacgggg aattcaagga tattgcccat cctatcaatg
     4981 ctgccacacg agaaaaaatc cagaaagcta ttctggacaa atatatcgca acgacaactg
     5041 cagaggagac aacggcttct tccgaaggac cggcggatca gacggacgga tcaactgaat
     5101 catctgagga atgatttata taaaaggggc actttgaaca gaagtgtctt tttttatttt
     5161 ttactattat aaagttgaaa tacaaaatca aaagtgtgat aatatatgcg gatctgaaag
     5221 gaatcgcatg tatggagagt attactgcgc ttatcctcgc ggcaggcgag ggaaagagga
     5281 tgaaatcgga taaagccaag gtgctgcatc aggtctgcgg aaagcccatg atcgaatggg
     5341 tatatgaggc tgcccgcgat gcaggagttg ggaaatgtgt catcatcgtc ggccataagg
     5401 ccgaacaggt gatggaacat atgggagaca gagccgagta cgtgatgcag gaacagcgcc
     5461 ttgggacggg acatgctgtg cagcaggcca ggagctatat ggaggatgaa gggtacatac
     5521 tcatccttta cggcgatact cctcttatca cctcggagac gataaaaaag gcaatggact
     5581 gtatgctgga cgaaaagctg gatgcggtgg tcatctccgc agatgtggag gacccttccg
     5641 gatacggccg aatcgtcagg aatccggacg gcagttttaa gcggatcgtc gagcaccggg
     5701 acgcaagccc tgccgaaagg gaaataaagg aaataaattc agggatgtac atcttcaaag
     5761 caagggaact ggctgatg
//
LOCUS       DAOSCC010000156         5764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8841_length_5764_cov_3.977404, whole genome shotgun sequence.
ACCESSION   DAOSCC010000156 DAOSCC010000000
VERSION     DAOSCC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8841_length_5764_cov_3.977404"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1544)
                     /gene="gguA"
                     /locus_tag="PLG72_08530"
     CDS             complement(9..1544)
                     /gene="gguA"
                     /locus_tag="PLG72_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ80879.1"
                     /translation="MSEYILEMKNITKEFPGVVALSNVTFQVRKGEIHALVGENGAGK
                     STLMNVLSGIYPYGTYSGEIIFKGAECRFRNIRDSEAAGIAIIHQELALNPFMTIAEN
                     IFLGNERARHGMINWDETNRKAAELMKRVGLNEKHTTLVKNIGVGKQQLVEIAKALGK
                     NCELLILDEPTAALNDDDSDHLLELLQTLNREHGLTCIMISHKLHEVIKIAHSITVLR
                     DGKTIESLDNSVHVSESRIIKGMVGRELTDRFPKRTPNIGEVGFEIKDWVVYDPMNEE
                     RVVIDHVNIYVRKGEIVGLAGLMGAGRTELAMSVFGRAYGKKISGKLIKDGKEITLNN
                     ISQAIANGIAYVTEDRKTAGLIHIQDIKRNITLANLKALTSNFVINEDEEILIGKDYR
                     ERFAIKSTSVFQKVGNLSGGNQQKVVLSKWIFAAPDVLILDEPTRGIDVGAKYEIYKI
                     ILQLADAGKCIIMISSELPEILGITDRLYIINEGRVVGEMKTSEATQEEIMSTIIRSL
                     GGR"
     gene            complement(1632..2714)
                     /gene="chvE"
                     /locus_tag="PLG72_08535"
     CDS             complement(1632..2714)
                     /gene="chvE"
                     /locus_tag="PLG72_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ80880.1"
                     /translation="MAKRSIVLLLVLAMLVVVVTGCGQATSGKGKLVGVTMPTKSLQR
                     WNEDGANVKKALEAKGYRVDLQYADNKQETQNSQIENQITMGCDVLVIASIDGGALGG
                     VLEQAKKKNIPVIAYDRLITNTENVDYYATFDNYGVGKMQGEFIVQALGLDKGEKGPF
                     TMECFGGDPGDNNAYLFNKGAMDVLQPYIDDGILVVKSGQTKFPDQIAITNWESKGAQ
                     DRMDNLLTAYYADENIDVVLSPNDSLAQGIVASLKSAGYGTASKPFPILTGQDCDKIN
                     VGQIIRGEQSMSIFKDTRILADQVIAMTEAILEGREVPTNATYDNGVKQVPSFNCEIK
                     FANKDNWKELLVDSGYYQLSDIPDAQ"
     gene            3124..5289
                     /gene="feoB"
                     /locus_tag="PLG72_08540"
     CDS             3124..5289
                     /gene="feoB"
                     /locus_tag="PLG72_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ80881.1"
                     /translation="MGLTSQSTGFGALKEMPELHVDSPDDTVIALAGNPNTGKSTVFN
                     SLTGLKQHTGNWPGKTVVNAQGKYRHNGRNCCLIDLPGTYSLLANSAEEEAARDFICF
                     ADPDVTVVVTDATCLERNLNLVLQILEITGNVVVCVNLMDEAGRKKIRINLKKLSQIL
                     GVPVVGTNARKNEGLRELKDAVHRVANGYQKTRPVKVSYDEAVEKAIALLQPALEELA
                     AGRINSRWAALRMIEGEKGLLDSLCKYLGFEDANSEEAAGYKELERKIGEARALLAES
                     GIGTEAVRDVVASAIVKTAEKISREVVTFETENYNRLDRRIDSVVTSRTWGIPIMLVM
                     LGIVFWITIKGANYPSELLADGLFRLQGHLSGLLMKAGAPGWLHDMLILGMYRTLAWV
                     VSVMLPPMAIFFPMFTLLEDLGYLPRVAFNLDNFFKKACAHGKQALTMCMGFGCNAAG
                     VIACRIIDSPRERLIASVTNNFVPCNGRFPTLIALSAIFIAEAAGAFRSVAAAVSVLA
                     VILLGVVITLLISKLLSKTILKGLPSSFTLELPPYRKPQLGKILVRSLLDRTLFVLGR
                     AVIVAAPAGMVIWVMANVSVDGISLLDHCAGFLDPFGRLIGLDGYILMAFILGLPANE
                     IVIPIIIMSYMSSGAILEFDSLDDLRRLLADNGWTWLTAVCTMLFSLCHFPCATTLLT
                     IRKETGSWKWTGLSFLIPTITGIVICFTVTQAVRLLGLV"
     gene            complement(5325..5639)
                     /locus_tag="PLG72_08545"
     CDS             complement(5325..5639)
                     /locus_tag="PLG72_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HOJ80882.1"
                     /translation="MKKVKITVMRRTVYQDLIDEYENPLENACDMKTGQVFIANGWRK
                     PEGLCDSAWESMSAFVMALAHGAEGLYDGWMKNRKSVMISCNDGFRPVSFLIEALDEE
                     AD"
BASE COUNT         1247 a         1564 c         1408 g         1545 t
ORIGIN      
        1 tttcccccct atcgtccgcc caagcttctg attattgtcg acatgatttc ttcctgggta
       61 gcttcgctcg ttttcatctc acccacgaca cgcccttcgt tgattatata caggcggtcg
      121 gtgataccca gtatctcagg cagttcggaa gatatcatga ttatgcattt ccctgcatca
      181 gccaactgca ggattatctt gtatatctca tattttgcgc cgacatcgat gcctcgtgtc
      241 ggctcgtcaa gtatcagcac atcaggtgcg gcaaatatcc acttgctcag cacgaccttc
      301 tgctggttgc ctccggaaag gttgcccact ttctggaaca cgcttgtcga tttgatagcg
      361 aaccgttccc tgtaatcctt gcctatcagg atctcttcat cctcgtttat gacgaagttg
      421 ctcgtcaacg ctttcaggtt cgccagcgtg atgttccttt tgatgtcctg tatgtgtata
      481 agccctgccg tcttcctgtc ttcggtgaca taggctatgc cgtttgctat tgcctggctg
      541 atgttgttca gcgttatttc ctttccatcc ttgatcagct taccgcttat ctttttgccg
      601 taagccctgc cgaacacact catcgcaagt tcggtcctcc ctgctcccat aaggccggca
      661 agaccgacta tctcaccttt cctgacatag atgttcacat ggtctattac gaccctctct
      721 tcattcatgg gatcataaac gacccagtct tttatttcga accctacctc acctatattg
      781 ggagtacgct ttggaaagcg gtcagtaagt tcgcgcccga ccattccctt gattatcctg
      841 ctttcactga catgcacgga gttgtcaaga gactcgatgg tcttcccgtc cctgaggacg
      901 gttatgctgt gcgcgatctt tattacctcg tgcagcttgt gcgatatcat tatgcaagtc
      961 agcccatgtt ccctgttcag cgtctgcaaa agttcgagaa gatggtcgga atcatcgtcg
     1021 ttcagggccg ccgtcggttc atccagtata aggagttcgc agtttttgcc aagcgccttg
     1081 gctatctcga caagctgctg cttaccgacg cctatgtttt tcaccagtgt cgtgtgcttt
     1141 tcattcagcc cgaccctctt catcagttcg gcagctttcc tgttggtctc atcccagttg
     1201 atcattccgt gccgggctct ttcattccca aggaatatgt tctctgcgat ggtcatgaac
     1261 gggttgagcg caagctcctg gtgtattatc gctatgcccg ccgcttcgct gtcacgtata
     1321 ttcctgaatc tgcactcggc acccttgaat atgatctcac ctgagtatgt accataggga
     1381 tatatcccac tgaggacgtt catcagcgtg gatttgccgg ctccgttctc tccgaccagt
     1441 gcatgtatct cgcctttcct gacctggaag gtgacattgg aaagagcgac gaccccggga
     1501 aactctttgg tgatgttttt catttcaagg atatattccg acattttccg ctcctcttca
     1561 acgatagatt aagcattttc cctttttatt aaaatggcgg cagacaagct gccgccagcc
     1621 tcaggacagt attactgagc atccggtata tcggacagct gatagtagcc gctgtcgaca
     1681 agcagttctt tccagttgtc tttgtttgcg aacttgatct cgcagttgaa tgaaggaact
     1741 tgctttacac cgttgtcgta cgttgcgttc gtcggaactt ccctgccctc cagtatagcc
     1801 tctgtcatgg caataacttg gtccgcgagg atcctggtat ccttgaagat ggacatagac
     1861 tgttcgccgc ggatgatctg gcctacattg atcttgtcac agtcctgccc ggtgaggatc
     1921 gggaacggtt tggacgccgt gccgtaacct gcggacttca gcgaagcgac gataccctgt
     1981 gcaaggctgt cattcggcga gaggacaacg tcgatgttct catcggcata atatgcagtc
     2041 agaaggttat ccatcctgtc ctgcgcaccc ttcgactccc agttcgtgat agctatctgg
     2101 tccgggaact tggtctgacc tgacttgaca acgaggattc catcgtctat gtacggctgc
     2161 aggacatcca tagctccctt gttgaacagg tatgcattgt tgtcgcccgg gtcaccgcca
     2221 aagcattcca tagtgaaggg acctttctcg cctttgtcaa ggccgagagc ctgtacgatg
     2281 aactcaccct gcatcttgcc gacgccatag ttgtcgaatg ttgcatagta gtcaacgttt
     2341 tcagtgttgg ttatcagcct gtcataagca attacgggaa tgttcttttt ctttgcctgc
     2401 tcaaggactc cgcccagtgc gccgccgtcg atcgaagcaa tgaccagcac gtcgcatccc
     2461 atggttatct ggttttcgat ctggctgttc tgggtctcct gtttgttgtc ggcatactgg
     2521 aggtcgaccc tgtagccttt agcctccagg gctttcttga cattggcgcc gtcttcattc
     2581 catctctgga gcgatttcgt aggcattgtg actcccacga gctttccttt gcctgatgta
     2641 gcctgaccgc agcccgtcac tacaaccacc agcattgcaa gcaccagtag cagtacgatt
     2701 gaccttttcg ccataaccat tcctcccata tataatattc aagtaaattc tataattaga
     2761 tatggcagtc ctgaatagag ataattatcc aatgatggta atttattatc tggcaaaaat
     2821 taaccggctt tcatatctgg aattttttaa tgttaaaaaa tcacaggcat gaaagccggt
     2881 aaaacagcgg atcgtggcgt ttatgtactg taacttgcaa gccgagtaat gtatggcaac
     2941 gggatctgcc gttgcccctg catattttat gctgttatag cacagcggtg ctgtacgttt
     3001 ccgccgtcct attttacccc ggcaggtaac acatgattcc ggcggttcat atattagccg
     3061 taggcaaatc atttattgct ggtattaaag cagacagcat caccatcgag gaagggatac
     3121 acaatggggc tgacaagtca atcgaccggt tttggtgccc tgaaggaaat gcccgagctg
     3181 catgtcgact ctcccgacga cactgtaata gcccttgcag gaaatcccaa taccggaaaa
     3241 agcacagtat tcaacagcct tacagggctc aagcagcata cggggaactg gcccgggaaa
     3301 actgttgtca atgcacaggg aaagtacagg cataatggga ggaactgctg tctgatcgat
     3361 ctgcccggta catattcatt gctcgccaac tcggcagagg aagaagcggc cagggatttc
     3421 atctgcttcg cggatcctga tgtgaccgta gtcgtgacgg atgcgacatg cctggaaaga
     3481 aacctgaacc tggtgctcca gatcctggag atcacaggca atgtagttgt atgcgtcaac
     3541 ctgatggatg aagccggaag aaaaaaaatc aggatcaacc tgaagaagct ttcacagata
     3601 ttgggcgtgc cggtcgtcgg taccaacgcc agaaagaatg aaggcctgag ggaacttaaa
     3661 gacgccgtgc accgtgtcgc caacggatac cagaaaacca ggcctgtgaa ggtttcatac
     3721 gatgaagctg tcgagaaagc catagctctc ctgcagcccg cattggaaga actggccgcc
     3781 ggcaggataa acagccgatg ggcggctttg cgcatgatag aaggagaaaa ggggttactt
     3841 gattctctct gcaaatacct gggttttgag gatgcaaaca gtgaagaggc tgccggttat
     3901 aaagaactgg aacggaaaat cggcgaagcc cgtgcacttt tggcggaatc cggcatcggc
     3961 acggaagcgg tccgcgatgt cgtggcttcc gccatcgtga agactgcgga aaaaatcagc
     4021 cgtgaagtgg tgacctttga aaccgaaaac tacaacaggc tggaccgcag gatcgactcc
     4081 gtcgtgacct ccaggacatg ggggatcccg ataatgcttg tcatgcttgg gatcgtgttc
     4141 tggatcacca tcaagggagc gaattaccct tcagagttgc tggcagacgg cctgttccgg
     4201 ctccagggcc atctgtccgg cctgttgatg aaagcaggag cgccgggctg gctgcatgac
     4261 atgcttatcc tgggcatgta ccgaacgctt gcctgggtcg tgtcagttat gctgcctcct
     4321 atggcaatat tcttcccgat gttcacgctg ctggaagatc tcggctacct tccaagagtt
     4381 gccttcaatc tcgacaactt cttcaagaag gcatgcgccc atgggaagca ggcacttaca
     4441 atgtgcatgg ggtttggctg caatgccgcc ggagtcattg cctgccgtat catcgactct
     4501 ccccgtgaaa ggctgatcgc atctgtcact aataacttcg ttccctgcaa cggccggttc
     4561 cctacattga tcgcactgtc cgccatattc atcgcggaag cggccggagc cttcagatcc
     4621 gttgccgcag cggtctcagt actggcagtc attttgctgg gcgtagtgat cacacttctg
     4681 atatcgaaat tgctgtcaaa aaccatcctc aaagggctgc cgtcttcctt cacgctggaa
     4741 cttcctccat accggaagcc ccaacttgga aagatcctcg tccggtcgct gctggacagg
     4801 acactgttcg tccttggccg cgccgttatc gtggcagcgc ctgccggtat ggtcatctgg
     4861 gtgatggcca atgtgtccgt ggacggcatc agcctgctgg accactgtgc gggattcctg
     4921 gatccctttg gccgtctgat cgggctggac ggctatatcc ttatggcttt catcctggga
     4981 ctgcccgcga atgagatcgt cattccaata atcatcatga gttatatgtc atcaggtgca
     5041 atactggaat tcgacagcct ggacgacctt cgccggctgc ttgcggataa cggatggacc
     5101 tggctgacgg ccgtctgcac catgctgttt tcactgtgcc atttcccatg cgcgacgacc
     5161 ctgctgacca tccgtaagga aacaggcagc tggaaatgga ccgggctctc attcctgata
     5221 ccaacgatca ccggcatcgt catttgcttt accgtgacac aggccgtgcg gctccttggt
     5281 cttgtatgaa catcggcgcc cggttgccgg acgcgcatca tttttcagtc cgcttcctca
     5341 tccagtgcct cgatcaggaa gctcacgggg cggaagccgt cgttgcacga tatcatgacc
     5401 gacttcctgt tcttcatcca tccgtcgtac aagccttctg ctccgtgggc aagagccatc
     5461 acgaaggcgg acatgctctc ccatgcacta tcgcacaggc cttccggctt cctccatcca
     5521 ttggcgataa aaacctgtcc ggttttcatg tcgcaggcat tctccaatgg gttttcatac
     5581 tcgtctatca ggtcctgata gactgttcgc ctcataactg taattttcac tttcttcata
     5641 cggatcactc cctatcctgc actgatacgt tcttctccca gtcatctctc gtaaggcagg
     5701 taacgtgcag tgtgcggata tctccggtca gccgccatag gacatccttc cttgtatggt
     5761 gata
//
LOCUS       DAOSCC010000157         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8888_length_5740_cov_4.226913, whole genome shotgun sequence.
ACCESSION   DAOSCC010000157 DAOSCC010000000
VERSION     DAOSCC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8888_length_5740_cov_4.226913"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLG72_08550"
     CDS             complement(<1..399)
                     /locus_tag="PLG72_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ80883.1"
                     /translation="MAQNIAVLGSTGSIGVQTLDVVRNLGIRVSALTAYSNVDLLEQQ
                     AREFMPRLVSVGDGVLAADLKDRLKGLKIEVLSGTDGIRAAAGIDGVDTVVVAIVGIA
                     GLLPTVEAIRKGRKVALANKETLVTAGRIVM"
     gene            complement(432..1259)
                     /locus_tag="PLG72_08555"
     CDS             complement(432..1259)
                     /locus_tag="PLG72_08555"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ80884.1"
                     /translation="MKTRVISAIVAIAILAAVICLGETAIGIAVFLLAVMAMHEFNRA
                     LEKGGYMPVKAISFAACAPLLYIALSGILPSGAVISGNGVVIAAAVFAFILLVSLFCF
                     LMFSDGKYSIKDISVTLFGIMYIPFLFSFFTLTRQMEAGYLYIWLILIGACATDTFAF
                     FTGLAVGRVKIVPKISPKKTLEGCIGGVAGCVISMTVFGACFRNGMDVPLMQFAVLGF
                     ICGIISQLGDWSASAVKRAAGIKDYGNIMPGHGGVLDRIDSILFTAPAVYFYINLFF"
     gene            complement(1261..2007)
                     /locus_tag="PLG72_08560"
     CDS             complement(1261..2007)
                     /locus_tag="PLG72_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOJ80885.1"
                     /translation="MRLFRKKAKSTGFDNSLNVPVHIAVIMDGNGRWAAKRGLPRNAG
                     HREGANTLKRLVKECNAIGIKYLTAYAFSTENWNRPKEEVDGLMDLLLEFLRNAEKEL
                     AGTNIRISVIGDTGKLSEEIRKEIRRVVANTAANTGLNLIIALNYGSRCEIAAAAARI
                     AKDAKEGRLDPEDVDEELFSSYLYTSGIPDPDLVIRPSGEYRLSNFLLWQSSYSELWF
                     SNVLWPDFSKEHLYQAIRDYQHRNRRFGGL"
     gene            complement(2084..2278)
                     /locus_tag="PLG72_08565"
     CDS             complement(2084..2278)
                     /locus_tag="PLG72_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80886.1"
                     /translation="MKDVSGFRLEDAMKQLKAQGYEITVRLTAAPRLRDRGYDADSRV
                     VRQRLLDEKTVELLVCNVNS"
     gene            complement(2284..2835)
                     /gene="frr"
                     /locus_tag="PLG72_08570"
     CDS             complement(2284..2835)
                     /gene="frr"
                     /locus_tag="PLG72_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ80887.1"
                     /translation="MSEYKDIENKMVKAIGALKEELVGIRAGRANPAILDKITVDYYG
                     VPTPINQLGSISVPEARVIVIQPWDAKILKDIEKAIQKSDIGINPSNDGKVIRLIFPV
                     LTEERRKELTKQVKKIGENAKIAIRNVRRDALEDFKTQKKNGEITEDDLKVAENDIQK
                     LTDKYIEEVDKLVELKEKEIMEV"
     gene            complement(2867..3571)
                     /gene="pyrH"
                     /locus_tag="PLG72_08575"
     CDS             complement(2867..3571)
                     /gene="pyrH"
                     /locus_tag="PLG72_08575"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ80888.1"
                     /translation="MALKYKRVLLKLSGEALAGDRKAGIDTEVLNEVAEKIRQAYEMG
                     AEIAIVVGGGNFWRSRSGVGMDRTTADHMGMLATVINALALQDALESKGVPTRVQTAI
                     EMRQIAEPYIRRKAVRHLEKKRIVIFACGTGNPYFSTDTTAALRAAEIDADVILKATM
                     VDGVYDSDPKVNPNAVRFESISFAEALNLRLGVMDSTAISLCMDNKLPLIVFDLSDPE
                     NILRVMKGEKIGTTLY"
     gene            complement(3681..4328)
                     /gene="tsf"
                     /locus_tag="PLG72_08580"
     CDS             complement(3681..4328)
                     /gene="tsf"
                     /locus_tag="PLG72_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ80889.1"
                     /translation="MITAEMVRQLRERTGSGMMDCKKALTEANGDMEKAIEILRERGL
                     AAAAKKAGRIAAEGIVDAYIHGNGRIGVLVEVNIETDFAAANEEFKQLVKDIAMQIAA
                     ARPEYVRREDVPAEVIEKEMNILRVQAKNEGKPDKVIEKMVEGRMEKFYKEVCLLEQP
                     WIKDPDKTIKQLVAEKIATIGENINIRRFARFERGEGLEKKQENFAEEVAKQIGG"
     gene            complement(4639..5325)
                     /gene="rpsB"
                     /locus_tag="PLG72_08585"
                     /pseudo
     CDS             complement(4639..5325)
                     /gene="rpsB"
                     /locus_tag="PLG72_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT         1221 a         1660 c         1206 g         1653 t
ORIGIN      
        1 catgacgata cggcctgccg ttacaagtgt ttccttgttt gcaagcgcca ccttcctgcc
       61 tttgcgtatg gcttcaacgg taggcagcaa tccggctatg cctacaattg cgaccactac
      121 tgtgtcaacg ccgtcgatgc ccgcggcagc cctgataccg tcagtgccgc taagaacttc
      181 tattttcagg cctttcagcc tgtctttaag atcggccgca agaacgccgt ctccgaccga
      241 aaccaggcgg ggcatgaatt ccctggcctg ctgctcaagc aggtccacat ttgagtatgc
      301 tgtcagagcg ctcaccctga tgccaaggtt cctcacgacg tcaagagtct gtactcctat
      361 ggaacctgtt gaaccgagca cagctatgtt ttgtgccatt gcgaactcct tcccgaaagt
      421 ttggaccttt ttcagaagaa aaggtttatg taaaaatata cggcgggagc agtgaaaagt
      481 atgctgtcta tcctgtccag cacccctccg tgtccgggca ttatattccc gtaatccttg
      541 atccctgccg ctcgtttgac tgccgatgcc gaccagtcgc ccaactgcga tattatgccg
      601 catatgaacc cgagcactgc aaactgcatc agcggtacat ccatcccgtt tctgaagcag
      661 gcgccgaaaa ccgtcattga gatgacgcat ccggcaactc cgccgataca cccctcaaga
      721 gttttctttg gacttatctt tggtacgatc tttacccttc cgacagccag acctgtaaag
      781 aaggcgaacg tatcggtcgc gcaggcgccg atgaggatca gccagatata cagatatcca
      841 gcttccatct gcctggtgag cgtaaaaaag gaaaacagga acggtatgta cattataccg
      901 aacaatgtga ccgatatatc ctttatactg tatttcccgt ctgaaaacat caaaaaacag
      961 aaaagcgaca ccaacaggat aaaagcaaaa accgcagccg caatcacgac gccgtttcca
     1021 ctgatcaccg caccggaagg aagtatgccc gagagggcta tgtacagcag cggggcgcat
     1081 gctgcaaaac tgattgcttt tacaggcatg tatcctcctt tttccagcgc cctgttgaat
     1141 tcatgcatcg ccatcactgc aagcaggaag acagctatgc caatggcagt ctccccaaga
     1201 cagatcactg ctgcaagtat tgctatcgct acaatggcac taatgaccct ggtcttcatt
     1261 ctataaccct ccaaaacgcc tgtttctgtg ctgataatcc ctgatagcct ggtacagatg
     1321 ctccttagag aaatccggcc acaaaacgtt cgagaaccac agttccgagt atgatgactg
     1381 ccagagcagg aaattgctca gcctgtactc gccgctcggc cttatcacaa gatccggatc
     1441 aggtatcccg ctggtataaa gataacttga aaacagttct tcgtccacgt cttccgggtc
     1501 aagccttcct tctttggcat ccttcgcgat gcgggccgct gccgcggcga tctcgcacct
     1561 gctgccgtaa ttgagggcaa tgatcagatt cagcccggta tttgcggccg tatttgccac
     1621 tactctcctg atctctttcc ggatctcctc cgagagtttg cccgtatcgc ctatcaccga
     1681 aatcctgata ttcgtccctg ccagctcttt ttccgcattc cgcaggaact ccagcagaag
     1741 atccatgagg ccgtccacct cttcctttgg ccggttccag ttctccgtgg aaaaggcata
     1801 tgccgtcaaa tattttattc cgatggcatt gcattctttg accagccttt tgagcgtatt
     1861 ggctccctct ctgtgccctg cgttcctggg caggcctctt ttggccgccc atctgccatt
     1921 gccatccatg attacagcaa tatggacagg cacattgagg ctgttatcaa agccagtgct
     1981 cttggctttc ttcctgaaca accgcatctg ttacctccgg gaaatacaca aatagcgcaa
     2041 atactgccgt aaataaacaa ggctgtctat acagccttgt aaatcagcta tttacgttgc
     2101 acaccaaaag ctcaactgtt ttttcatcca acagcctttg cctgactact ctcgaatccg
     2161 cgtcgtaccc cctgtccctc agccttggtg cagccgtgag acggacagtt atctcgtagc
     2221 cctgagcttt cagttgtttc atggcatcct ccagcctgaa gccggagaca tccttcatcc
     2281 ggattatacc tccattatct ccttctcttt gagttccacc agcttgtcga cctcttctat
     2341 gtacttgtca gtcagcttct ggatatcatt ttccgctact ttcaggtcat cttccgtaat
     2401 ctcgccgttc tttttctgtg ttttgaaatc ttccagtgcg tctctcctga cgttgcgtat
     2461 cgcgatcttg gcattctcac cgatcttttt cacctgtttg gtgagttctt tccttctttc
     2521 ttcggtgagc accgggaaaa tcagccttat aacctttccg tcattgctgg ggtttattcc
     2581 gatgtcagat ttctggatcg ctttttcgat atccttgagt atctttgcat cccagggctg
     2641 tataactatg acccttgcct ccggtactga aatgctgccc aactggttga ttggcgtggg
     2701 tactccgtag taatccacgg ttatcttgtc taaaatagcc ggattggccc tgcctgcgcg
     2761 gattccgacc aactcttcct tcaatgcgcc tatcgccttg accattttgt tttcgatatc
     2821 cttgtattct gacatatgaa accctccttt ttatggatta tttggatcag tataatgttg
     2881 ttccgatttt ctcgcctttc atgaccctca gtatgttttc agggtcgctc agatcgaaaa
     2941 cgatcagcgg cagtttatta tccatgcata aagagatcgc tgtcgagtcc attacgccta
     3001 atctgaggtt aagcgcctct gcgaacgata tcgattcaaa acggacagca ttcggattca
     3061 ctttcgggtc ggaatcatat acaccgtcca ccatggtcgc cttgagaatg acatccgcat
     3121 ctatctcggc agctctcagc gccgctgtcg tatcggtaga gaaataaggg tttccggttc
     3181 cgcatgcaaa aatgactatt ctctttttct cgagatgcct tacagccttc ctccttatat
     3241 agggctctgc tatctgtctc atctcgatgg ctgtctgaac tctcgtagga acgccttttg
     3301 attccagtgc atcctgcagt gccagagcat ttataactgt cgccagcatc cccatgtgat
     3361 cagccgtcgt tctgtccatg ccgacgccgc tcctgcttct ccagaagttg ccgccgccta
     3421 ccacaatcgc gatctcggct cccatttcat aggcctgtct tatcttctca gctacttcat
     3481 tcagaacttc cgtatctata ccggccttcc ggtcccccgc gagcgcttcc ccgctaagct
     3541 taagaagcac ccttttgtat ttcagagcca tatgatagtt acccccgcct ttataaattg
     3601 ttcttcaatg aacacaaaaa atcctgccga cgcactaaaa agggaacata ttttgtatgc
     3661 tccctttttt tcgatctcta tcagccaccg atctgctttg caacttcttc tgcaaagttc
     3721 tcttgcttct tctccaaccc ttctcccctt tcgaaacggg caaatcttct gatgttgata
     3781 ttttcgccta tcgttgcgat cttttcagcg acaagctgtt ttatcgtctt atccggatcc
     3841 ttgatccagg gctgctcgag caggcagact tctttgtaga acttctccat cctgccttcg
     3901 accatcttct ctatgacttt gtccggtttg ccttcattct ttgcctgtac tctgagaatg
     3961 ttcatttctt tctcgataac ctcggcagga acatcctccc tgcgcacata ctcaggtctt
     4021 gcagcagcta tctgcatagc gatatccttt accaactgct tgaattcctc atttgcagcg
     4081 gcaaagtcgg tttcgatgtt tacttccacg aggacgccta tcctgccgtt gccgtgtatg
     4141 taggcatcca cgatgccttc tgccgcgatc cttccggcct ttttggcagc ggcggccagt
     4201 cctctttccc tcaatatttc aatcgccttt tccatatcgc cgttagcttc agtcagggcc
     4261 tttttgcagt ccatcatccc tgaacctgtt ctttcacgga gctgtcttac catttcagca
     4321 gtaatcatca gtattcctcc aaacactaat atttatatta ttaaccgcac aggcggccgt
     4381 tattcccaaa cactccaggt atatccgccc ggctccctct caggaaccgg gcggcccttt
     4441 gccgtattac tcagcagagc tttcagttgc ttctgcttcc gcagcggctt ctccggagac
     4501 ttccgcagca gtttctccag cagcttctgc cgccggctct tctgcgatct ccgctgcagc
     4561 atcagcagct tctccggtca tgtcagttcc atccgtctca tggatcttgt cttcgccgtc
     4621 tgcttctccg gttgagaatt gctcaccctg attagcctct ataacggcat ccgcaatttt
     4681 cgacgcgatc agcttgactg ccctgatggc atcgtcattg cccggtatga cataatccac
     4741 ttcgtcagga tcacagttcg tgtcgactat tgccactacc ggtatgccga gtttccttgc
     4801 ttcgaggata gcattccttt ccttcctggg gtctacgacg aacagcgctc caggcagctt
     4861 cttcatgtcc tttatgccgc cgaggtattt ctcgagcttt tccatctcac cgcgcagctt
     4921 gatgacttcc ttcttcggaa gaacgtcaaa caggccgttc tgctccatgg cttcaagctg
     4981 tttgagcctt tcgatcctct gctggatcgt cctgaagttg gtgagcatac cgccgagcca
     5041 tctggcgttc acatagaact gtccgcatct cagggcttca tccctgatag aatcctgggc
     5101 ctgtttcttc gtacccacga aaaggatgct gtcaccgttc atgatgactt ctcttacaaa
     5161 gaaataggct tcttcgattt tcttgacggt tttctgaaga tcgatgatgt agatgccgtt
     5221 cctctcagtg aagatgtatt cggccatctt ggggttccat ctcctggtct ggtgtccgaa
     5281 gtggactcct gcttccagga gctgtttcct tgaaataact gacatatata acctccttaa
     5341 gtttttgcca ccacagccgt catccgccca gaacaccgga aacggcactt ttctgcgcgt
     5401 cctgccgtgt gtgtgatttt tttctttgat agtataacac acagttaatg aattatcaat
     5461 caaaaaattg ggtgaaaacg cacagcagga tgataccctt catcatcagt tccaaatcaa
     5521 ataaaaactg acctgcagcc gggctgcggg cggccggtca gccatttggg ctgcttcata
     5581 aacaactgtt ggcaggctta cctgctccga tcgcaattct gatagtcatc gggactcatg
     5641 tgaagccggg agattggccg ttcatgcatt tgggttgctt cgtgaacagc tactggccgg
     5701 ctcatttgct ccaatgaggg cgcaggtcac tcgccgtccg
//
LOCUS       DAOSCC010000158         5730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8904_length_5730_cov_3.893744, whole genome shotgun sequence.
ACCESSION   DAOSCC010000158 DAOSCC010000000
VERSION     DAOSCC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_5730_cov_3.893744"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLG72_08590"
     CDS             complement(<1..384)
                     /locus_tag="PLG72_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ80890.1"
                     /translation="MAGKSRTKLIIAAVIAVLLIATFLFADDAARVLYPLKYHDLIRK
                     YSERYNLDPYLVLAVIKAESSFRHTAVSRKNARGLMQITEGTGKWSAEKIGLKDFTVD
                     MLFDPEININIGCWYLSRLYSEFGDT"
     gene            complement(409..1017)
                     /gene="coaE"
                     /locus_tag="PLG72_08595"
     CDS             complement(409..1017)
                     /gene="coaE"
                     /locus_tag="PLG72_08595"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520919.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ80891.1"
                     /translation="MKIIGVTGGIGSGKSSVARILKDLGAAVIDADVLAKSVTSAGGK
                     AFDELVGYFGEEIVGDDGEIDRKKLAHAAFSDEEKLKMLNAITHKHIAEKIYDTVEVL
                     KNTGKWDTIVIDAPIPIEKGFIDLADEIWVVVADRETRIRRAMERSGYTYDEVAARID
                     SQMSEDEYLELADEVIRNGDSIEELEQTVVRLFLSKKQKWQQ"
     gene            complement(1049..3739)
                     /gene="polA"
                     /locus_tag="PLG72_08600"
     CDS             complement(1049..3739)
                     /gene="polA"
                     /locus_tag="PLG72_08600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOJ80892.1"
                     /translation="MEKLMLIDGNSILNRAFYGLQGPQLLTAADGLHTNAIYAFLNIL
                     NKYLAEEDPRYICVAFDLKAPTFRHREFADYKANRKGMPPELAEQVPYTKEILDAMNI
                     KRLEMEGFEADDIIGSVSKCAEGREMQVVIVTGDRDSLQLASEKTRIIIPVTRAGRTE
                     TEYYDAAAVFERYGVTPAQLIDVKALMGDKSDNIPGVPGIGEKTALELIQAYGTLEGV
                     YENIDKVSKKSVREKLEANRDLAFMSRRLATIDRNMPELCSLDELKRKEFDDNRLYKL
                     FNRLGFRSLIEKFGLDTKERINVVKAELPEFTAVEDPIALAGLAASMAEAKEISICHL
                     LDHTSRFETGLAGIAFSWAGQNAYVNVGRGISEEQFLEVFGKLFSDPAVKKYGHNLKN
                     LVVYLKRKGVQLDGLCFDTMLGAYILDPSRSTYALSDLSQLYLGYSFESLEDLCGKGK
                     NFTPYHMLPAEQVAPVAVMHASSTAKLAEAIRTQIEANGQEKLCYEIEMPLIEVLADM
                     EYRGFRVNRKELEEYSARLDEKIEAAEKDIYDLAGETFNINSPKQLGAILFEKLGLPV
                     IKKTKTGYSTDAEVLDQLADEHEIVSRILEYRQLIKLKTTYADGLLNMINPATGRIHS
                     SFNQTVTATGRISSTEPNLQNIPVRLEMGREIRKVFVPENENFLLTDADYSQIELRVL
                     AHIAADENMRTAFRNNEDIHTATAAGVFRVPKEQVTPLMRTRAKAVNFGIVYGIGDFS
                     LSRDLGITRKQARKYIDDYLDRYPGVKAYMHDVVEYGRQHGYVETILGRRRYLPELKS
                     SNFNTRSFGERVAMNMPIQGTAADIIKIAMVNVYKKLRARKMISALILQVHDELIVET
                     SIDEKEEVEKILKDCMENAVRLEVPLVAEVRTGSSWYETK"
     gene            complement(3870..5048)
                     /locus_tag="PLG72_08605"
     CDS             complement(3870..5048)
                     /locus_tag="PLG72_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ80893.1"
                     /translation="MMNCADVRENISAYADDELSAGERQLFEEHISSCPECRKELDDM
                     VRIIKLCSSIPLHDLPEGFRDELHEKLTAAVSKNHGPAGADKPKRKWTARTFASIAAG
                     ILLIFLGGSIVRLGLLSVKLGAKSSAPAETASAAGAAESPAETTADGSAGIAMAEAED
                     SVPIQFSEATGEMRDTTEAEMNSMEPGKLFASQRSSEANRSCEDDERINGLGLVMAEN
                     QTINYKTSEMYVNAEDPAAALETVMTLPTDNNGTLYSDENDLYGGGQRYGQGGYLDKP
                     QFDVSQAGSMTQLKLVLTFTEMDYDNFTAALNDTFGAANVQTGAFVIEDRTDELNKLA
                     EQANSYDQAIKDLEEKGDKGSAEEIDKLKKEKEDADRRIESLRLNSDFITVDVYINKK
                     "
     gene            complement(5068..5664)
                     /locus_tag="PLG72_08610"
     CDS             complement(5068..5664)
                     /locus_tag="PLG72_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ80894.1"
                     /translation="MDQNERLLISKAKAGDVEAFEQLIEAYQKKVYNLALRMTGNQDD
                     AADLAQEAFIRVFRSISGFKEQSSFSTWVYRITTNVCLDEIRKRKNRKVISIDEEIHM
                     DDGEIKRQVISEEPLPDELAEREELRSIVNAAINSLPEDQRIVLTLRDLNGLSYDEIA
                     KILDCPGGTVKSRINRARQALRNVLSARTELLIDDYVK"
BASE COUNT         1170 a         1593 c         1212 g         1755 t
ORIGIN      
        1 cgtatcgccg aattcactgt aaagcctaga aaggtaccag catcctatat tgatattgat
       61 ctcgggatcg aacagcatat ctaccgtgaa atccttaagt ccgatctttt cagcgctcca
      121 ctttcctgtc ccctcggtta tctgcatcag gccccttgcg tttttgcgtg aaacggcggt
      181 atgcctgaaa ctgctctccg cttttatcac tgcgagtaca aggtacgggt caagattata
      241 gcgctcggaa tacttccgga taagatcgtg gtatttcagc ggataaagaa ctctggccgc
      301 atcatcggca aacaggaagg tcgcaataag caaaacggcg attactgcgg caattatcag
      361 ctttgttctg cttttacctg ccaaatgaac acacctctcg atccacgttt actgctgcca
      421 tttctgcttt tttgacaaaa acagtctgac caccgtctgt tccaattctt cgatactgtc
      481 accgttcctt atcacttcat cagccagctc caggtattcg tcttcgctca tctgcgaatc
      541 tatcctggct gctacttcgt cataagtata tccgcttctt tccatcgccc gcctgatcct
      601 ggtctccctg tccgcaacaa cgacccatat ctcatccgcc agatcgataa accctttctc
      661 tataggtatt ggtgcgtcga tcacgatggt atcccatttt cctgtgttct ttaatacctc
      721 cacggtatcg tatatctttt ccgctatatg tttatgggtg atggcgttga gcattttcag
      781 tttttcctcg tctgaaaacg cggcatgtgc cagcttcttc ctgtcgattt ccccatcatc
      841 acccactatt tcttcgccaa aataacctac aagttcatca aaagcttttc ctcctgcaga
      901 tgttacgctt ttcgccagta catcggcatc gataactgcg gcgccaaggt ctttcaatat
      961 ccttgccacc gagctttttc cgctgccgat gccgccggtt accccgatta ttttcaacat
     1021 taccatcctt tccagccatt gttctgttct attttgtctc ataccagctg gatcctgtcc
     1081 tgacctcagc aactaagggc acttcaagcc ttaccgcgtt ttccatgcag tccttcagta
     1141 ttttttccac ctcctctttc tcatcaatgc ttgtttcgac gatcagttcg tcatgtacct
     1201 ggagtatgag cgccgatatc attttacgtg cacgaagttt cttatataca ttgaccatcg
     1261 ctattttgat gatgtcggcg gcagtcccct ggatcggcat gttcattgcg acccgctcgc
     1321 cgaaagaccg ggtattgaaa ttggacgatt tcaattccgg aagatatctc ctcctgccca
     1381 aaatagtctc cacatagccg tgctgcctgc catattccac tacatcgtgc atgtaggctt
     1441 tgacgcctgg atatctgtcc agatagtcat cgatgtattt cctcgcctgc ttcctggtga
     1501 tgcccagatc ccgtgaaagg ctgaaatctc ctatcccata cactataccg aaattgacgg
     1561 ctttggcccg tgtacgcatc agcggtgtga cctgctcctt cggcaccctg aaaacacctg
     1621 ctgcggttgc ggtgtgaata tcttcattat ttcggaatgc tgtcctcata ttttcatctg
     1681 ccgctatatg agccagcacc ctcagttcta tctgtgaata gtcagcatca gtcagcagga
     1741 aattttcatt ttccggtacg aacaccttcc ttatttccct gcccatctcc agcctgacag
     1801 gtatattctg cagattcggt tccgtgctgc tgatccttcc tgttgcagtg accgtctggt
     1861 tgaaacttga gtgtatgcgc cccgtcgcag ggttgatcat attgagcagc ccgtctgcat
     1921 acgtggtttt aagcttgatg agctgcctgt actcaagtat cctggaaacg atctcatgct
     1981 cgtctgccag ttggtcgagc acttccgcat ctgtcgagta acccgtcttg gtctttttta
     2041 tcaccggaag cccaagtttt tcgaaaagta tcgcgccaag ctgtttgggc gagttgatgt
     2101 tgaatgtctc gccggccaaa tcatatatgt ctttctctgc cgcttctatt ttttcgtcca
     2161 gccttgccga gtattcctca agctcctttc tgtttaccct gaatcccctg tattccatat
     2221 cagccagtac ttctataagc ggcatttcta tctcatagca cagcttttcc tggccgtttg
     2281 cctctatctg cgtcctgatc gcttccgcaa gttttgcggt gctgcttgcg tgcatcacgg
     2341 cgacaggagc tacctgctct gccggcagca tgtgatatgg agtaaagttt ttgcctttgc
     2401 cgcagagatc ttccagggat tcgaacgaat agcccaggta aagctgcgac aggtcggaca
     2461 atgcatatgt gctccgggaa ggatccagta tataggcgcc gagcatggta tcaaagcaca
     2521 atccgtcaag ctgtactcct ttgcgtttaa gatacactac gaggttcttg aggttgtgcc
     2581 cgtatttttt tacagcggga tcggagaaca gctttccgaa tacttcaaga aactgttctt
     2641 cactgatgcc tcttcctaca ttaacatagg cattctgccc cgcccacgag aaagctatcc
     2701 ctgccagccc tgtctcgaac cggctggtat gatccagcag atggcatatc gagatctctt
     2761 ttgcttccgc catggacgcc gccaggccgg caagcgcaat tggatcctca accgccgtaa
     2821 actcaggaag ttcggccttc accacattta tcctttcttt cgtatcaagg ccgaattttt
     2881 ctatcaggct cctgaatccc agcctgttga aaagcttgta cagcctgtta tcgtcaaatt
     2941 ctttcctctt cagttcatcc aatgagcaaa gctccggcat gttcctgtcg atggtggcaa
     3001 ggcgtctgct catgaatgcc aggtccctgt tggcctccag tttttcacgc acggattttt
     3061 tgcttacttt atcaatattt tcatatactc cttccagggt cccgtatgcc tggatcagtt
     3121 caagcgccgt tttctccccg atgccgggga ctccgggtat attgtcggat ttgtcgccca
     3181 tcagggcttt aacgtctatc agttgcgcgg gtgtgacccc gtagcgttcg aacactgccg
     3241 ccgcgtcata gtactcggtt tctgtcctgc cggccctcgt aacgggtatt atgatccttg
     3301 tcttttcaga tgcgagctgc aatgaatctc tgtcgcccgt cactattacg acctgcatct
     3361 ccctgccttc cgcgcatttt gaaacagagc cgattatatc gtccgcttcg aatccttcca
     3421 tttcaagacg ttttatattc atcgcatcca gtatttcttt cgtatatggg acctgctccg
     3481 ccagttcggg aggcatccct ttcctgttcg ccttatagtc cgcaaattcg cgatgtctga
     3541 atgtcggtgc cttgaggtcg aaagccacgc agatataccg tgggtcttcc tccgcgaggt
     3601 atttgttcag tatattcagg aacgcgtata tcgcgttggt atgaagcccg tccgccgcag
     3661 taagaagctg cggaccctga agtccgtaaa aagccctgtt gagtatgctg ttgccgtcga
     3721 tcagcatcag tttttccata cggcacctcc atagcccgga tatttctttt tacaatcata
     3781 tgtttttcca caggtgctgt ttttcatcca atatattcta cacaaaaaaa cggctgccgt
     3841 aaatggccgc cgttttcagc agactatgac tattttttgt taatatacac atctaccgtg
     3901 ataaagtcgg aattgagcct tagtgattcg atgcgtctgt ccgcatcctc tttttccttt
     3961 ttcagtttgt caatttcctc tgcgctgcct ttgtcgccct tctcttccag atcttttata
     4021 gcctggtcgt atgaattggc ctgttctgcc agcttgttca gttcatctgt ccggtcctca
     4081 atgacgaatg cgccggtctg aacatttgcc gctccgaacg tatcgttgag cgcagccgtg
     4141 aaattgtcgt aatccatttc ggtaaaggtc agtaccagtt tgagctgtgt catggagccc
     4201 gcctgcgaaa cgtcaaattg cggtttgtcc aaataaccgc cctggccgta gcgttggccc
     4261 ccgccataaa gatcgttttc atccgaataa agcgtaccgt tattgtctgt tggcagcgtc
     4321 atgacagttt ccagcgccgc cgcgggatcc tccgcgttga cgtacatctc cgaggtttta
     4381 tagtttattg tttgattttc agccattaca agacccagtc cattgatgcg ctcgtcatct
     4441 tcacatgaac ggtttgcctc ggaagacctc tgagacgcaa ataatttgcc cggttccatt
     4501 gaattcatct cagcttcggt tgtgtcccgc atttctccgg ttgcctcgct gaactggatc
     4561 gggacggaat cttctgcttc agccatggct attcctgcgc tgccgtctgc agtggtttcg
     4621 gccgggcttt ctgctgcgcc tgctgcagat gccgtttctg ccggagcgct ggattttgcc
     4681 ccgagtttga ctgaaagcaa tcctaatctc acaatgctgc ccccaaggaa gataaggagt
     4741 atgcctgctg caatcgatgc aaaggttctt gcagtccatt tcctcttcgg cttatcagcg
     4801 cctgccggac cgtgattctt cgatactgcc gcagtcagtt tttcatggag ttcgtccctg
     4861 aagccttccg gaagatcatg caaaggtatg ctgctgcaga gctttattat cctgaccatg
     4921 tcatccagtt ctttcctgca ctcgggacag ctgcttatgt gttcttcgaa caattgccgt
     4981 tcaccggcac ttaattcgtc atctgcatat gcgctgatat tttcccttac atccgcacag
     5041 ttcatcatct ccaaccctcc tttcacgtta tttgacgtaa tcgtcgatca aaagttccgt
     5101 tcttgctgac aaaacgttgc gaagcgcctg ccttgccctg ttgatcctcg atttgaccgt
     5161 acctcccggg caatcgagga tcttcgctat ttcatcgtaa ctcaggccgt tcaggtccct
     5221 cagtgtaagc actatcctct gatcctccgg caggctgttt atcgccgcat tcactatgct
     5281 tcggagttcc tccctctctg caagttcatc ggggagcggt tcttcgctga tcacctggcg
     5341 ctttatttca ccgtcatcca tatgtatctc ttcatcgatc gaaatcactt tcctgttctt
     5401 tctttttctt atttcatcga ggcatacgtt tgtagtgatc ctatagaccc acgtggaaaa
     5461 ggacgattgc tccttgaaac cggagatcga cctgaatacc ctgatgaagg cttcctgggc
     5521 cagatcggct gcatcgtcct ggttgcctgt cattctcagt gcaaggttgt atactttttt
     5581 ctgatatgct tctatcagct gctcaaaagc ttcgacatcg cccgccttgg ccttactgat
     5641 caaaagcctt tcattttggt ccataggctt ttgtgacccc ctgcagaaaa taagtgcata
     5701 taataagcac ccatgttaga ttatacatta
//
LOCUS       DAOSCC010000159         5725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_8914_length_5725_cov_5.111817, whole genome shotgun sequence.
ACCESSION   DAOSCC010000159 DAOSCC010000000
VERSION     DAOSCC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8914_length_5725_cov_5.111817"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..909
                     /locus_tag="PLG72_08615"
     CDS             97..909
                     /locus_tag="PLG72_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOJ80895.1"
                     /translation="MGKERLDILLVKKGLSESIEKARSSIMAGLVYVDGEKADKAGAQ
                     YPSDAEIVLKESEIPYVSRGGLKLEKAIRFFGINLDGKTAIDIGASTGGFTDCMLKNG
                     AAKVVAVDVGYGQLAWVLRNDERVINLERTNIRRLKPEDIGFKADFASIDVSFISLKK
                     VIPPVRELLKPGTGEIVCLIKPQFEAERNQVGRHGVVRDIGVHKQVVRDIVSFALETG
                     FFFKGITYSPIKGPEGNIEYLCYLSMKDGSAEISEILPAIDEAVQWSHIELG"
     gene            995..1879
                     /locus_tag="PLG72_08620"
     CDS             995..1879
                     /locus_tag="PLG72_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ80896.1"
                     /translation="MKKIGIITNADKDKDFAYTKLLAESIRRHGGSPMMTDVLSEASS
                     TGVPCGSEQDLIDSCDYLVCLGGDGTFLKAARKIYKKQIPIMGINLGNLGYLTEVDRN
                     NIDNAAAQLMEGKFEIEKRMMLEAVVTRKGKDTITDVALNDVVIARGAVSRILHVKAY
                     INDVFVDTFPGDGLIIASPTGSTAYSLSAGGPIAEPDTDVMIVTPICPHILYSRSFIT
                     KSDSVVKAVVADDFDHSAMVSLDGRDGYEISNGDYVSVRKSGHSINLIRIGKVNFFNI
                     LRTKIYFRGESLKKDEIQ"
     gene            1866..2318
                     /locus_tag="PLG72_08625"
     CDS             1866..2318
                     /locus_tag="PLG72_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOJ80897.1"
                     /translation="MKYSRHAKILELIEKYPIETQEELAEKLREHGMDVTQATVSRDI
                     KELRLIKVMADDGSYRYASITRTEGNISNKLLTVFSESFVSCDYANNIVVVKTLPGMA
                     QAAASAVDAMKWPEIVGSIAGDDTIMIVCRAEKIAEELVSKFNRLISV"
     gene            2397..4109
                     /gene="recN"
                     /locus_tag="PLG72_08630"
     CDS             2397..4109
                     /gene="recN"
                     /locus_tag="PLG72_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ80898.1"
                     /translation="MLLQLDIHDVALIDEISIELGEGLNVLTGETGAGKSIIIDSINA
                     VLGDRVSREIIRTGSEKALVEAVFRVDAERIRDIMNDMGMEPEEDGTLILSREISQSG
                     KNTCRINGKMVSATFMKAIGERLIDIHGQHDNQSLLKTECHIELLDSFAGESLRSVKE
                     EYARLLEQYRDVRAKLKALSGDPGERERKMDLLKFQVDEIRKARLKTGEDEELNKQKT
                     LLVNAGKISDTLAMVYDLLSGNSAGNSALDMMNVSLSQLSNIAGLDKSYGEMYSRLQD
                     LIYQLDDLAGEVRQYRESAEYDPAMLDEIEERLDLIFRLKRKYGSTIPEIHEFCQEAE
                     KQLEELEKSEETAKALTEELAKLEELLYQAALKLNAERMKAASLLESKIAQQLDDLEM
                     KKAQFRVDIQFDAEASVASGRKFGNNGLDKVEFLISPNVGEPLKPLARIASGGEMSRV
                     MLAIKTILAEVDRIPVLIFDEIDTGISGKAAQKVGEKLSYISRSHQVLCVTHQAQIAC
                     IADHNYLIEKTVEKEKTRTKVTRLGDDEKTKEIARLIGGETTEASLKYAEEMIKTAKK
                     TRTA"
     gene            4246..5529
                     /gene="spoIVB"
                     /locus_tag="PLG72_08635"
     CDS             4246..5529
                     /gene="spoIVB"
                     /locus_tag="PLG72_08635"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOJ80899.1"
                     /translation="MKKSAFIIKVLFILLSTAIAVTGISFMKVILTVPGEMVLIEGEE
                     YSYDIDSLFPISIKADTQGILRINGKSVERPYNHVGISKPVSLSSDRGGSLKLNLRFF
                     GIIPVKTVNVDVVSNKNLVACGNTIGVKIKLDGILVIGISSVISNGQKIVPARNTGIK
                     PGSKIIAVNGKETGSIDDLADAIEQSGGKPLKIKYKTEDEVQETMVTPIRSTEDGKYH
                     IGLWVRDSTAGIGTLTFYDADTGKFGALGHGITDIDTGTLMSVKTGEILESDILGVKM
                     SRSGVPGELKGVFSEGRHLGTILANTEVGIYGKLDSDAMQRLRGRVYPVGVRANIKEG
                     PATILSNIDGKKIEEYDIVIQKVSRQSLNGSKGMIIKITDERLLQTTGGIVQGMSGSP
                     VIQDGKIVGAVTHVLVNDPTRGYGIFIETMLMNAA"
BASE COUNT         1758 a         1169 c         1568 g         1230 t
ORIGIN      
        1 gtctgacggc ggcttgcacc gagagatccg cagggcatgt gacagctgta tcgatccggt
       61 tgccgggtca accgggattt attcgggagg aagtatttgg gaaaagaaag acttgatata
      121 ctgctggtta agaaaggctt gtctgaaagt atcgaaaagg ccaggagttc cataatggcc
      181 ggccttgtgt atgtagacgg cgaaaaggcc gataaggcag gagcgcagta tccctccgac
      241 gccgagatcg tcctgaagga aagcgagata ccctatgtga gcagaggcgg actgaaactg
      301 gaaaaagcca tccgtttttt cggcatcaat cttgacggaa agacagccat cgatatcgga
      361 gcgtccaccg gcgggtttac agactgtatg ctgaagaacg gagccgcaaa ggtcgtggcg
      421 gttgatgtgg gctacggcca gctcgcgtgg gtgctcagga atgatgaaag ggtcataaat
      481 ctcgaacgta cgaacatacg acggcttaaa cccgaagata tcggatttaa ggctgatttc
      541 gcatctattg acgtttcatt catatcgctg aaaaaagtga tcccgccggt cagggaactt
      601 ctcaagcccg ggacgggcga gatcgtctgc ctgataaagc cccagttcga agcggagcgg
      661 aaccaggtgg gcaggcacgg cgttgtaagg gatatagggg tccacaaaca ggttgtaagg
      721 gatatagtgt catttgcctt agagaccgga ttctttttca aaggtattac ctattctccg
      781 ataaagggcc ctgaagggaa tatagaatat ctttgctatt tgtcgatgaa agacggttcg
      841 gctgaaatat cggaaatatt acccgccatt gatgaagctg tacagtggtc tcatattgaa
      901 ttgggatagc ctattgggta taatgaaata agttgagcat cgcagcgttt ctttctgaag
      961 catggatccg gatgcttgtc cgtcggaggt cttgatgaaa aagatcggta taatcacaaa
     1021 cgcagataag gacaaggatt ttgcatatac gaagcttctt gccgaaagca tcagaaggca
     1081 cggaggaagc ccgatgatga cggatgtgct ctccgaggcg tcaagcacag gtgtgccatg
     1141 cggcagtgag caggatctga tcgattcctg cgattacctg gtgtgcctcg gcggcgacgg
     1201 gacatttctg aaagcagcgc ggaaaatcta caaaaagcag atcccgataa tgggcataaa
     1261 cctcggaaat ctcggatacc ttacagaagt ggacaggaat aacatagaca atgcagcggc
     1321 acagttgatg gaaggcaagt ttgaaataga aaaaaggatg atgctcgaag ctgtcgtcac
     1381 acggaaagga aaagatacaa tcacagacgt ggccctcaat gacgttgtca tcgccagggg
     1441 agctgtttcg aggatattgc atgtgaaagc gtacataaac gatgtgttcg ttgatacttt
     1501 ccccggtgac gggctcatca ttgcaagtcc gaccggatcg acagcctatt ctctgtctgc
     1561 gggaggtccg attgctgaac ctgatacgga tgttatgata gtgactccaa tatgccccca
     1621 tatactttat tccaggtctt tcatcactaa atccgacagt gtggtcaaag cggtagtagc
     1681 agacgatttc gaccactctg caatggtttc gcttgacggc cgggacggat atgagatatc
     1741 gaacggcgat tatgtcagcg taaggaaatc cggccattcg ataaacctga tcaggatagg
     1801 gaaagtaaac ttctttaaca tactaagaac caaaatatat ttcaggggag agagtctgaa
     1861 gaaagatgaa atacagtaga cacgctaaaa ttctggaatt gatagagaag tatcctatcg
     1921 agacccagga agaactggca gaaaaactga gagagcatgg aatggatgtg acccaggcca
     1981 ccgtttcgag agatatcaag gaactcaggc tgatcaaggt catggctgac gacggttcgt
     2041 acaggtacgc atcgatcact cgcacagaag gcaatatttc caacaaactc ctcacagtat
     2101 tcagcgaatc atttgtgagt tgtgattatg cgaacaacat cgttgttgta aagacgctgc
     2161 caggtatggc ccaggctgcc gcatcagcgg tagatgctat gaaatggcct gagatcgtag
     2221 gctcgatcgc aggcgacgat accataatga tcgtatgccg ggcggaaaag atcgctgaag
     2281 agctcgtcag caagttcaac aggttgattt cggtataatg ggccggcccg ggcatattcg
     2341 ggaacgatcg cggcgaagag acgccaggag tcaggttggg ggtgaattct gccaatatgc
     2401 ttctgcaact ggatatccac gatgttgcgc ttatagacga aataagcatt gaacttggcg
     2461 aaggcctcaa tgtgctcaca ggagaaacag gagctggtaa atcgataata atagattcca
     2521 tcaatgcagt ccttggagac agggtatcga gagagatcat ccggacaggt tccgagaagg
     2581 ctctggtcga agccgtattc cgggtcgatg cagagaggat aagagatatt atgaacgata
     2641 tgggaatgga acccgaagaa gacgggacgc ttatattgtc aagggagatc tcgcagtcag
     2701 gaaagaacac ctgcaggata aacgggaaaa tggtttcggc aacgtttatg aaagcgatcg
     2761 gagagaggct gatcgatata cacggacagc atgacaacca gtcgcttctt aagacggagt
     2821 gccatatcga gcttcttgat tcatttgcag gcgaaagtct ccgatctgta aaagaagagt
     2881 atgcgagact gcttgagcag taccgggatg tacgggcgaa gcttaaggcg ctttcgggag
     2941 atcccggtga acgtgaaagg aagatggatc tccttaagtt ccaggtcgac gagatccgga
     3001 aagcgagact aaaaacgggg gaagacgagg aactgaacaa acagaagaca cttcttgtca
     3061 atgcggggaa aatatcagat acgcttgcaa tggtatacga ccttttatcc gggaattccg
     3121 ccggaaattc cgcgctcgac atgatgaacg tatcgctttc ccaactcagt aacattgcgg
     3181 ggctggataa aagctacggg gaaatgtatt cccggctcca ggatcttata taccagcttg
     3241 acgatcttgc aggcgaagta aggcaatacc gtgaaagcgc agagtatgat cctgcgatgc
     3301 ttgacgagat cgaggaaagg ctggacctga tattcaggct caagaggaaa tacgggagta
     3361 ctatccctga aatacatgaa ttttgccagg aagccgaaaa acagctcgaa gaacttgaaa
     3421 agagcgagga aaccgcaaaa gcgctgacgg aggaactggc aaagcttgaa gagttgctgt
     3481 accaggcggc cctcaagctg aacgctgaac gtatgaaggc tgcttcgctg cttgagagca
     3541 aaatcgcgca gcaattggac gatctggaaa tgaaaaaggc acagttcagg gtggatatac
     3601 agttcgatgc agaagcatcc gtcgcttcgg gcagaaaatt cggcaataac ggcctggata
     3661 aagtggagtt cctcatatcg cccaacgtgg gagagcctct caaaccactg gcccgcatag
     3721 cgtccggcgg cgagatgtcg agagtgatgc tggcaatcaa gaccatcctc gcggaggtcg
     3781 acaggatccc cgtgctgata ttcgacgaaa tagatacggg gataagcggc aaggcagccc
     3841 agaaagtagg cgaaaaactg tcatacatat cccgaagcca ccaggtgctt tgcgtgacgc
     3901 atcaggcaca gatagcatgc atagcggacc ataattacct gatcgaaaaa acagtggaaa
     3961 aagaaaaaac caggacaaag gtaaccaggc tcggcgatga tgaaaagacg aaggagatcg
     4021 caaggctgat aggcggcgaa actacggaag catcgctgaa atacgcagaa gaaatgatca
     4081 aaaccgccaa aaagacgaga acggcctgat aaatcaagaa taagcaacaa aatccggctt
     4141 aatcgccgga tttttttctt ttctattcat ttttaccttc tgttcattta attgtttgaa
     4201 taattgattt tgcaaaaggt taacttactt gcaggaggtt gaacaatgaa aaaatccgca
     4261 tttatcataa aggtactttt tattttgttg tcaactgcaa tcgcggtaac cggcatttct
     4321 ttcatgaagg tcatcctgac cgttcccggt gaaatggttt taatagaagg agaagaatat
     4381 tcatacgaca tagacagtct tttccccatc agcataaaag ctgataccca ggggatcctg
     4441 aggataaacg gaaaaagcgt cgaaaggccc tataatcacg ttggcatatc caaacctgtt
     4501 tcgctcagtt cagacagagg cgggagcctg aaactgaacc tgcggttttt cggcatcatt
     4561 ccggtaaaga cggtaaatgt cgatgttgtt tcaaataaaa accttgtagc ctgcggcaat
     4621 accataggcg taaaaataaa actggacggc attcttgtga taggtatatc cagtgtgatt
     4681 tcgaacggac agaaaatagt tccggccagg aacacgggca taaagccggg aagcaaaata
     4741 atagccgtaa acggcaagga aacggggagc atcgatgatc ttgccgatgc gatagaacaa
     4801 agcggcggaa agcctctgaa aataaagtac aaaaccgaag atgaagtgca ggagaccatg
     4861 gtgacgccga tcaggtcgac cgaggacggc aaataccaca tcggtttatg ggtccgcgac
     4921 agtaccgccg ggataggcac gcttacattt tacgatgccg ataccggcaa atttggagct
     4981 ttgggccatg gcatcacaga tatcgacaca gggacgctga tgtcggtaaa aacaggagaa
     5041 atactggaat ctgacatact gggcgtgaaa atgagcaggt cgggcgtacc gggcgaactg
     5101 aagggagtct tttctgaagg caggcatctt ggtacgatac tggcgaatac ggaggtaggg
     5161 atatacggca aacttgacag tgatgcaatg cagaggctca gaggaagagt gtacccggtg
     5221 ggggtcaggg caaatataaa ggaaggcccg gccacaatac tgtcgaatat agacgggaaa
     5281 aagatagagg agtatgatat agtgatacag aaagtatcca ggcagagcct taacggctcg
     5341 aaagggatga taataaaaat aacggatgaa aggctgctgc agactactgg tggtatagtc
     5401 caggggatgt cgggaagccc ggtgatccag gacggaaaaa tcgtcggggc agtaactcat
     5461 gtactggtga acgatccgac gagagggtac gggatcttta tagaaaccat gcttatgaac
     5521 gcggcttgat aacttacagg aaataaatgg taattcacgg aaataaagca atgacaactg
     5581 aagcacaaac aaggttatat gtcggcgaaa ctgcttgaaa taaagggttt caataacaaa
     5641 aaacctgata tatatctata aatgagcaaa agcgctaaca tataatgtaa aattaaaggg
     5701 gacaccttga aaatccgata ttttt
//
LOCUS       DAOSCC010000160         5635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9069_length_5635_cov_3.312186, whole genome shotgun sequence.
ACCESSION   DAOSCC010000160 DAOSCC010000000
VERSION     DAOSCC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9069_length_5635_cov_3.312186"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLG72_08640"
     CDS             <1..683
                     /locus_tag="PLG72_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ80900.1"
                     /translation="ELPVEFIKLRRRRSSLPEIDPSVAAAIGATMTPTVGQRDINLLP
                     KLKEIREATLRRIKLTRVAALFAVVALLSLAIYGFLVLRQYGVTADTMSVQEEIIKYS
                     SINETKNRLRQANALLASAESVLEKYNSDVIPGTWIFDLINTAMPESVFAVSYSQTED
                     GKVNISGVSKNLTDLPDFVYNLKQSGKVEKAVLSAIISREAEDGSVLDYNFTIEIIPM
                     KAGAENEG"
     gene            673..1836
                     /locus_tag="PLG72_08645"
     CDS             673..1836
                     /locus_tag="PLG72_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80901.1"
                     /translation="MKVNLSNIRFKPQRGLTTREFIMLALLVVVLEGYFLINYLLAPA
                     YDNYASAVRELEERQQALDDIKRDYVRKSEIEEEIKEKEGKLAVIQAQLPPYVSQEEA
                     VFSLKDYSDRSGLNIKSISFIGAEELPLSVVPAGGTEDKAPFTAPVPVLVEQQVTINF
                     MGDYRQVYDFLSNVETNSRKGSLKSITLQKNDDGTLNGVMTLSFPSYWDEYEGRKPFV
                     MTPESESGKASLFDEYIGYSTSGQARTEVLKPAPRPDFYITLNSYLNNSAKVFMMNYY
                     NAGSEAIEDRNETVTAQMTLNGADGKYTFSYKLGAYDIVGDTPTEIKDGKIRMEVLVQ
                     PRRSDEDRVGLVLDITNNTGVPFEITVKGDDPSNPRFMTGRTVGNVMVIQANG"
     gene            1869..2825
                     /locus_tag="PLG72_08650"
     CDS             1869..2825
                     /locus_tag="PLG72_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ80902.1"
                     /translation="MIGSFTRKTAWNRLMKKLKAKAAQSSGFTLIEVIFAIIVLSIVS
                     ITLLEMFTVSTKTNAAAGVLDKARSLCVEASEEFRANPVCPDPMDPADDGESEYLRGF
                     KASSDVPGKITYTKYFNSDWEETGEDRAEYMLEIVSEEALADTIPASYYPAPAVRDRA
                     AGSPASIEVRTNETIRLARVGLSDCELTIDSNTYDIDTDADAIILIDADVAVAKTALI
                     PIHLDCRNVTGNIEVKVANEIGQLVIDGEVYEAIADIYLCDIPEGKNVTINVTRGVAT
                     SNEVSTAVQEMVRYNAAISVKEIGSGHTIAESSVETFLVKNR"
     gene            2841..5456
                     /locus_tag="PLG72_08655"
     CDS             2841..5456
                     /locus_tag="PLG72_08655"
                     /inference="COORDINATES: protein motif:HMM:NF022289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HOJ80903.1"
                     /translation="MKKRCFFAVLSGKLHNEKGNAAVITLSVILVLTALGTVSLLASA
                     MNVRMSGKTLSWSEEYYTLDTMAEKLVQKIEEEVLIPAEKDARTYVMNRLDRAKPEDL
                     YGLFHDDSLTPPGDPYQMYISYAQDYFNKYYLETWTYEAGGDRHEYTVHDYEQAIVDG
                     TFNGKTIEEISRKYVNDGSPAEDRAARAYTGDLLQYTEELFDRVYFHMVAKRLEYLHM
                     HDDDFSDAKENIVIKSIAYDENYGRREDTEQNRCTYRFSNIRDESPTRTISETWEHIK
                     PESGDITIYIRAWDPTNIRDKLVHVAVEVIRPAYETIEKTIYTPVYGNPIWANALTVR
                     GGIKIGEEAPAKAVIKGDVYASGASGEPYESGLTVFNNTTVDIYGNVYTAGNVQAVSI
                     REGNGGTLRVHTSETGSSGVSYNYKKAIYGNEYLHENHYAYDIDIEYTADQDAGAGSI
                     PFVFKDSVDQGNVYCDSLQVSEGVNGAYLEVNGNLWTMDDIQMDGEQSAISVGTTSEK
                     DGNTVAKYTYIGLNDESSRDDPNTSSSVINNFPFTSTGGTNSIIELNSNFVVPGVAFY
                     NFFDSPDPADRRNKYYKSVESVTSRTEKPVAIINAYLDSAGNIYYNQEGDEFALMAVN
                     EDEVNIDDQAKAIIRFINSLEGGVYTNIRTRLTEPAGYVAGVALVEKETGGAIVYTRR
                     PNIDETGSVVLTNASENWLAYSELTYSEKLFRLHMAKTKMLGRYVSEYNSSAEPIRFG
                     DLVDIGRLGSLDSSEIIYRSGRPGSEPLDIRGTMEGIVYCTGDLKITGSGEFRGAIIC
                     EGDVTIEGDVTIKYDENVIRRKLKNSARLREFFSKGQMGQKLFDIEEYSTTSGKRVQV
                     KRYRIAAWKETPRLR"
BASE COUNT         1651 a         1311 c         1549 g         1124 t
ORIGIN      
        1 cagaacttcc cgttgagttc atcaagctca ggcggagacg ttcctcatta ccggaaatag
       61 atccgtcggt cgctgcggcc ataggcgcca cgatgactcc gaccgtgggc cagcgggaca
      121 taaacctgct gccgaagctc aaggaaatca gggaagcgac cctgagaagg ataaagctga
      181 caagggtggc agcgctgttt gccgtggtag ccctcctgtc gcttgccata tatggtttcc
      241 tcgttctcag gcagtatggg gtgacggccg acacgatgag cgtccaggag gaaataatca
      301 agtattcatc gataaatgaa acaaagaaca ggctccggca ggccaatgct cttcttgcaa
      361 gcgctgagag cgtgcttgag aaatacaatt cggacgttat tcctggcacc tggatcttcg
      421 acctgatcaa cacggcaatg cccgaatcag tatttgccgt ttcatactcg cagaccgagg
      481 acggcaaggt gaacatttcg ggcgtctcga aaaatctcac tgacctgcct gacttcgtat
      541 acaacctcaa gcagagcggc aaggtcgaaa aggcagtcct gtcggctata atcagcaggg
      601 aggccgaaga cggcagtgtc ctcgactata acttcaccat tgaaataata ccgatgaagg
      661 caggtgctga aaatgaaggt taacctttcg aacatacgct tcaagccgca gagaggactg
      721 acgacccgcg aattcataat gctggctctc ctggtggtgg tcctggaagg ttatttcctg
      781 ataaactacc tccttgcgcc tgcctatgac aattatgctt ctgccgtcag ggaacttgag
      841 gaaaggcagc aggcgctcga tgatatcaag agggattatg tgcgcaagag cgagatcgag
      901 gaagagataa aggaaaaaga ggggaaactg gccgtaatac aggcgcagtt gcccccgtat
      961 gtgtcgcagg aggaggcggt cttctccctg aaagactatt cggacaggag cggccttaat
     1021 ataaaatcca tttctttcat cggcgctgaa gaactgcctc tttccgtggt gcctgccggc
     1081 ggcacggaag ataaagctcc cttcaccgca cccgtgcccg tactcgtaga acagcaggtg
     1141 accatcaact tcatgggaga ctaccgacag gtatatgact tcctttccaa cgtggaaacc
     1201 aactcaagga aaggatccct gaagtcgatc acgctgcaga aaaacgacga cggcactctc
     1261 aacggcgtga tgacactgtc gttcccgagc tactgggacg aatacgaagg ccgcaaaccc
     1321 ttcgtcatga cgccggaatc agaatccggg aaggcaagcc tttttgacga atatatcggg
     1381 tattcaactt ccggccaggc gcgcacagaa gtgctcaagc ctgcgcccag gcccgacttt
     1441 tacataacgc tcaacagcta tctcaataat tcggcaaagg tattcatgat gaactactac
     1501 aacgcaggct ccgaggccat cgaggacagg aacgagacag tgaccgcaca gatgaccctg
     1561 aacggggcgg acggcaagta tacgttcagc tataagctcg gcgcctacga catcgtcggc
     1621 gataccccga cggagataaa ggacgggaag ataaggatgg aagtgctcgt gcagccgaga
     1681 agatcggatg aggacagggt tggcctggtg ctcgacataa ccaacaacac cggcgtgccg
     1741 ttcgaaataa cggtaaaggg tgacgatcca tccaatccga gattcatgac cggaagaacg
     1801 gtcgggaacg ttatggtgat acaggccaac ggatgacacg gcacagggac attggagcag
     1861 gtggtacgat gatcggaagc ttcaccagga aaactgcctg gaacaggctg atgaaaaagt
     1921 taaaggcgaa agcggcgcag agcagtggct tcacgctgat cgaagtcata ttcgctatca
     1981 ttgtgctttc catcgtttcc ataacgttgc tcgagatgtt cacggtatcg acgaagacca
     2041 acgcggcggc cggcgtactc gacaaggcga gatcgctctg cgtggaagcc agcgaggaat
     2101 tccgggcaaa cccggtctgc ccggatccca tggatccggc tgacgatggc gaaagcgaat
     2161 atttgcgcgg cttcaaagcg tcatcagatg taccgggcaa gatcacatat acgaagtatt
     2221 ttaacagcga ctgggaagaa acaggagaag accgggccga atatatgctg gaaatagtct
     2281 ccgaagaagc attggcggat accatacccg ccagttacta tcctgcaccg gctgtcaggg
     2341 accgtgctgc cggttctccc gcatcgatag aagtgaggac caatgaaacg atcaggctgg
     2401 cgcgcgttgg tttgtcagat tgtgaactga ctatagattc aaacacttac gatatcgaca
     2461 ccgacgccga cgcgataata ctgatcgatg ccgacgtggc tgttgcaaaa accgcgctta
     2521 tcccgataca cctggattgc cggaacgtga caggcaacat cgaggtcaaa gtggcaaatg
     2581 agatcggaca actggtcata gatggggaag tctatgaagc gatcgcagat atatacctct
     2641 gcgacatccc tgaagggaaa aatgtcacga taaatgtaac caggggcgtt gccacctcga
     2701 acgaagtcag tacggcagtc caggaaatgg tcaggtataa cgccgctatc agcgtaaagg
     2761 aaataggcag cggacacacg atagctgaaa gcagcgttga aacctttttg gtgaagaacc
     2821 gataaccggg tggatgtgaa atgaaaaaaa ggtgcttttt tgctgtatta tccggaaaac
     2881 ttcataatga gaaaggcaat gcggcggtaa taacgctgag cgttattctt gtgctcacgg
     2941 cgctcggcac cgtatcgctg ctggccagcg ccatgaatgt caggatgagc ggcaagaccc
     3001 tgtcctggtc tgaggagtac tatacccttg atacgatggc tgaaaaactt gttcagaaga
     3061 ttgaagaaga agtactcata ccggccgaga aggacgcaag gacgtatgtg atgaacaggc
     3121 tggacagagc aaaaccggaa gatttgtatg gactgttcca tgacgacagt cttacacctc
     3181 ctggtgaccc ataccaaatg tatatttcct atgcacagga ttatttcaat aagtactatc
     3241 ttgagacatg gacatatgag gcaggcggtg acaggcacga atacacagtg catgattatg
     3301 agcaggccat cgttgatggt acattcaacg gcaagaccat agaagagatc tccagaaaat
     3361 atgtgaacga cgggtctcct gccgaagaca gggcagccag ggcgtataca ggtgaccttt
     3421 tgcaatatac ggaagaactt ttcgacagag tttatttcca catggtagcg aaaaggctgg
     3481 agtatttgca tatgcatgat gacgatttct ccgatgcaaa agaaaacatt gtaataaaga
     3541 gcatcgccta tgacgaaaat tacgggagaa gagaagacac tgaacaaaac aggtgtacat
     3601 acagattcag caatataagg gatgaatccc ccacacggac catcagtgaa acctgggaac
     3661 acataaagcc ggagagcgga gatatcacca tttatatacg tgcctgggac cctacgaata
     3721 ttcgtgacaa gttagtgcat gtggcggtcg aggtcatccg gcctgcctat gagaccatag
     3781 aaaagaccat atacacaccg gtctacggca acccgatatg ggcaaatgct ctgaccgtga
     3841 gaggcggcat caaaataggc gaggaggccc cggcgaaagc tgtgataaag ggtgatgtat
     3901 atgcgtcagg tgcatcgggc gaaccttatg agtccgggct aactgttttc aacaacacta
     3961 ctgtggatat atacggcaat gtgtacacag ccggcaatgt acaggccgtg agcataagag
     4021 aagggaacgg cgggacgctg agagtgcata catcagagac tggttcgtca ggagtctcgt
     4081 acaactataa gaaagccata tatggaaatg aatacctgca tgaaaaccat tatgcgtatg
     4141 atatcgacat tgaatataca gcagaccagg atgcgggggc aggcagcata ccgtttgtct
     4201 tcaaagactc agtcgatcag ggcaatgtgt actgcgacag cctgcaggtg tccgaaggag
     4261 tgaatggagc atatcttgag gtgaatggca acctctggac catggacgac atccagatgg
     4321 acggggaaca gtcggccata agcgtcggga cgaccagcga aaaagacggc aatactgtgg
     4381 cgaagtatac gtatatcggt ctgaatgacg agtcgtcgag agatgaccca aacaccagca
     4441 gttcggtgat aaacaatttc ccgttcacct ccacaggagg gacaaacagt atcatcgagc
     4501 tcaacagcaa cttcgtcgtt ccgggcgtgg ccttctacaa ctttttcgac agcccagacc
     4561 cggctgacag gagaaacaag tattacaagt ccgtcgaaag cgtcacgtcc aggacagaaa
     4621 aacctgtggc gataatcaac gcctacctgg attcggccgg caatatctat tacaatcagg
     4681 aaggcgatga gttcgcactg atggcagtta atgaagatga agttaatata gacgatcagg
     4741 cgaaagcgat tatacgtttt ataaatagtc ttgaaggtgg ggtttacacc aacatcagga
     4801 ccagactgac ggagccggcg ggttacgtag ccggtgtggc gctggtcgaa aaggaaaccg
     4861 gcggtgcaat tgtgtataca cgcaggccga acattgatga aacaggttcg gtggtactga
     4921 ccaatgcgag cgaaaactgg ctcgcgtaca gtgaactgac gtacagcgaa aaacttttca
     4981 ggctccatat ggccaagacg aaaatgctcg gcaggtacgt cagcgaatat aattcttccg
     5041 ccgaaccgat aagattcggt gatcttgtcg acattggcag gctgggcagc ctggacagtt
     5101 cagaaatcat ttatcgtagc ggcagacctg gcagcgaacc gcttgacata cgcgggacta
     5161 tggaaggaat agtttactgc accggcgacc tgaaaataac gggcagcggt gaattcaggg
     5221 gagcaataat atgtgaaggc gatgtcacga tagaaggcga cgtcacgatt aagtatgacg
     5281 aaaacgtgat acgccgtaaa ctcaaaaaca gcgcaaggct cagggaattc ttcagcaaag
     5341 ggcaaatggg acagaaactg tttgacatag aggagtattc gaccaccagc ggcaaacgcg
     5401 tccaggtgaa gcggtacagg atagccgcgt ggaaagagac tccaaggctg agatgacaga
     5461 tatctcagac aggacaagca gcccgggcat gacaggtatc tcaaaacgac actgaaaaac
     5521 ggaaaggtca gaggtaatgg agggaggcct tatgaaaaaa agatccaaca gagtgacagc
     5581 tatagtgaca ttactgatca tgataataac gcttttggtg ccggcgggcg catac
//
LOCUS       DAOSCC010000161         5622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9092_length_5622_cov_4.600323, whole genome shotgun sequence.
ACCESSION   DAOSCC010000161 DAOSCC010000000
VERSION     DAOSCC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_5622_cov_4.600323"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLG72_08660"
     CDS             <1..955
                     /locus_tag="PLG72_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ80904.1"
                     /translation="LSKLGIEKQWLADLYESQEVSGYVSQEAAALTGLKAGTPVVGGG
                     GDQAAGAVGNGIVKPGVVSSTIGTSGVVFAYLDKIRIDPLGRLQTFCHAVPNTWHVMG
                     VTQAAGFSLKWFRDNLCSDEMSVAGLMDTDPYVLMDKEAEKVKPGSNGLIYLPYLIGE
                     RTPHLDSDARGVFFGLSTVHRKSDIIRSVMEGVVFSLKDCLSIIADLGVEINEVRASG
                     GGGKSKLWKQMQADVFGIPITTTNSSEGPALGVALLAGVGTGVYKDVAEACDVAIKPV
                     EVQQPDAGNTEVYSKFYGLYCQLYKSLKQDFKDLAKIMKSI"
     gene            965..2164
                     /locus_tag="PLG72_08665"
     CDS             965..2164
                     /locus_tag="PLG72_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ80905.1"
                     /translation="MTKGKTGNSKFLKAYNETGILDLIRTKQAVSRAELSKETGLSPT
                     AIGVIVSSLMEKEYIHEIGTGESSGGRKPVLLELKPDSWYSVGLDIEVDIVQYVLMDI
                     TGRVLHEGSVPLRVNTPEMTVMASAACVRNIMQKFSIKKERLLGVGISVPGIVDSKSR
                     RVVLAPNIGWEDADPAEMLKAKLGLPVYIENEAMASAICENWIGSCQTAGNFVCLNIK
                     SGIGAGIFTGGRPYRGTNGSAGEVGHIVVDENGPRCGCGNYGCLETLASTSSIIEKAR
                     RIVRQGVVSSLNEIENIDEITLEDIAKAARAGDAAAMNVLEEATRYIGIALSNLINTL
                     NPSMIVLGKDFTVYGDLVLGTLRSIIDAKALRRPASNVEIVISKLGERASTLGAAIIP
                     LKKLFGR"
     gene            2225..3547
                     /gene="xylA"
                     /locus_tag="PLG72_08670"
     CDS             2225..3547
                     /gene="xylA"
                     /locus_tag="PLG72_08670"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOJ80906.1"
                     /translation="MAEYFKGVQKIKYEGKGSDNPLAFKYYNPDEVVAGKRMEDHLRF
                     AVAYWHTFQATGTDPFGPVGTAIRPWSHISDPMDLAKAKVEACFEFCEKLGVPFFCFH
                     DRDIAPEADTLRETNKRLDIIVAAIKDRMKNSTVKLLWGTTNAFGHPRFTHGASTSPH
                     AEVFAYAAAQVKKAMEITKELGGQNYVFWGGREGYETLLNTDMKLELDNMARFLRMAV
                     EYAKEIGFEGQFLIEPKPKEPTKHQYDFDSATVIGFLKTYGLDPYFKLNIEANHATLA
                     QHTFQHELCVARINNMLGSIDANQGDLMLGWDTDQFPTNIYDTTLAMLEVLKMGGFTK
                     GGLNFDAKVRRGSFEPEDMFYAHIAGMDAFAKGLKVAARIMEDGKFDKFIADRYSSYT
                     TGIGKDIVENKVGFKELEQYVLDLGKVNIKSGRQEMLEVWLNSYILEE"
     gene            3753..4292
                     /locus_tag="PLG72_08675"
     CDS             3753..4292
                     /locus_tag="PLG72_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HOJ80907.1"
                     /translation="MLEDRMYSAGSGGWRTVKPYDRDEAVRYAHKWAYGRNPAYYDFE
                     KLGGDCTNFASQCIYAGSGVMNHTPVYGWYYINSYNRTASWTGVNYLYKFLINNKGSG
                     PFAEEVDIGGVMPGDIAQLSFSDSKHFQHSPVIVKVSDPPTMEGILVAAHTFDTDYYR
                     LSDFEPVHVRFLHIAGVRV"
     gene            complement(4364..4849)
                     /locus_tag="PLG72_08680"
     CDS             complement(4364..4849)
                     /locus_tag="PLG72_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ80908.1"
                     /translation="MGVKQYVVFLVDGDEFGLEIEKVSIIERMLEIFRIPNTPGYIEG
                     LANLRGKVHTIFNLRKRFHQPCPEFNEDNRIIIAKTSDDSVVGLIVDSVREIVKIEES
                     QFEPVPSALSSVRDKFLSGTAKIDDRLILLLDLDKVITEENVPPEKPAKKNSTKKAEK
                     S"
     gene            complement(4897..>5622)
                     /gene="argJ"
                     /locus_tag="PLG72_08685"
     CDS             complement(4897..>5622)
                     /gene="argJ"
                     /locus_tag="PLG72_08685"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOJ80909.1"
                     /translation="IELELQDTRVVIGGMAKGSGMIHPNMATMIGIITTDANISRKLL
                     DKALRTVVRSSFNRITVDGDTSVCDMVVIFANGMAENPGIITEGEDYEKFVKGLEYVC
                     TYLARLIAKDGEGATKLIEVSVEGAENPDDAYKAACAVAKSPLVKTMMFGNDVNWGRI
                     MTAVGYSGAEFDPEYVDIYIGDIPMCKAGCAVDFDEAEARKLLEQDEVKVLIKLNKGV
                     YSDRVWTCDFSYDYVKINASYRT"
BASE COUNT         1417 a         1387 c         1538 g         1280 t
ORIGIN      
        1 gctctcgaag cttgggatag agaagcagtg gctggccgat ctgtatgaat cccaggaggt
       61 aagcggctat gtcagccagg aggcggcagc gctgacagga ctgaaagccg ggacgccagt
      121 tgtcgggggc ggcggtgacc aggcagcagg cgctgtcgga aacggcatcg tcaaaccggg
      181 agtagtatcc tcgaccatcg gtacttcagg cgtcgtattc gcttatcttg acaagatcag
      241 gatagatccc ctggggaggc tccagacatt ctgccatgct gtcccgaata cctggcacgt
      301 catgggcgtc acacaggcag ccggcttctc gctgaaatgg ttcagggaca atctgtgcag
      361 tgatgaaatg agcgttgccg gtcttatgga cacggatcca tatgtactga tggataagga
      421 agctgaaaag gtgaaaccgg gaagcaacgg cctgatttat ctgccttacc ttatcggcga
      481 aaggacgccg catcttgatt cggatgcaag aggcgtattc ttcggacttt caacggtaca
      541 caggaagagc gatattatca gatccgtcat ggaaggcgtc gtcttcagtc tcaaagactg
      601 cctgagcatt attgcggacc ttggcgttga gataaacgag gtgcgtgctt cgggcggcgg
      661 cgggaagagc aagctgtgga agcagatgca ggctgatgta ttcggcatcc cgataacgac
      721 caccaattcc agcgagggcc ccgctcttgg cgttgcgctg ctcgcaggag taggtacggg
      781 cgtttacaag gatgtggccg aggcatgcga cgtggctatc aagcctgtag aagtccagca
      841 gccggacgcg gggaacacgg aagtgtactc gaagttctac ggcctgtatt gccagcttta
      901 caaatcactg aagcaggatt tcaaggacct ggcaaagata atgaaaagca tttgagggtt
      961 atagatgaca aaagggaaga ccggaaacag caaatttctg aaagcttata acgaaacagg
     1021 catattggac ctgatccgca cgaaacaggc tgtttcaaga gcggagcttt ccaaggaaac
     1081 aggattgtcg cccactgcca taggcgttat agtgtcttcg ctgatggaaa aggagtatat
     1141 ccacgagatc gggacaggcg agtcgagcgg cgggagaaaa cctgtgctcc tggaactgaa
     1201 gccggactca tggtattcgg tcggattgga tatagaggtc gacattgtcc agtacgtttt
     1261 gatggacata accggccggg tgctgcatga gggctcggtg ccgctgcgtg tgaacacgcc
     1321 cgagatgaca gtaatggcct cggcggcatg tgtcaggaat ataatgcaga agttctccat
     1381 aaaaaaggaa aggttactgg gagtggggat ctcagtgccc gggatagtgg actccaaatc
     1441 ccgcagggtc gtattggctc cgaatatcgg atgggaagac gcggatcccg ccgaaatgct
     1501 aaaggcaaag ctcgggctcc ctgtctacat cgaaaacgaa gcaatggcct ccgccatttg
     1561 cgagaactgg ataggatcat gccagacagc cggcaacttt gtctgcctta acatcaaatc
     1621 aggtataggc gcgggcattt tcaccggagg caggccttac agggggacca acggaagcgc
     1681 cggcgaagtg gggcatatag tcgtcgatga aaacgggccc aggtgcggct gcggcaacta
     1741 cggatgcctt gagacgctgg cttccaccag cagcatcatc gaaaaagcca gaaggatagt
     1801 caggcagggt gtggtttcca gcctgaacga aatagaaaac atcgatgaga tcacactgga
     1861 ggatatagcc aaagccgcaa gggcgggaga cgcagcggcg atgaatgtgc ttgaggaggc
     1921 gacccgatac atcggcatag cattgtccaa cctgatcaac acgctcaatc cgtcaatgat
     1981 cgtactgggc aaggatttca ccgtatacgg cgaccttgta ttgggcacgc taagaagcat
     2041 tatagacgcc aaggcgctca ggaggcccgc ttcgaatgtc gagatcgtaa tatcgaagct
     2101 gggcgaacgg gcttccacgc tgggagcggc gataatccct ctcaagaagc tgtttggaag
     2161 ataggctgtt catgcggcgc atgagtatgc gcgagtcaaa ataaatcaaa atggaggtta
     2221 gatcatggct gagtatttta aaggtgtaca aaaaatcaag tacgaaggca aaggttccga
     2281 caacccgctg gcattcaaat actacaaccc ggatgaagta gtagcgggca agaggatgga
     2341 ggaccacctt aggtttgccg ttgcatactg gcatactttc caggcgaccg gaacagaccc
     2401 gttcgggccg gtaggcactg ccataaggcc ctggagccat atctccgatc cgatggacct
     2461 ggcaaaggca aaagtcgaag cttgcttcga attctgtgaa aagctcggcg ttccgttctt
     2521 ctgtttccat gacagggaca tagctcctga agccgacacg ctgagagaga ccaacaagag
     2581 gctcgacatc atcgtggctg caataaagga caggatgaag aacagcactg taaaacttct
     2641 gtggggaacc accaatgctt tcggacaccc gaggttcact cacggcgctt cgacgtctcc
     2701 ccatgctgaa gtgttcgcat atgctgctgc ccaggtcaaa aaggcaatgg agatcacgaa
     2761 ggaactcggc ggccagaact acgtattctg gggcggcagg gaaggttacg agacactgct
     2821 caacaccgac atgaagctgg agctcgataa catggcacgt ttcctcagga tggcagtgga
     2881 atatgcgaag gagatcggtt tcgaaggcca gttcctgatc gagcccaagc cgaaggaacc
     2941 cacaaagcac cagtacgatt tcgattcagc aacagtaata ggcttcctca agacatacgg
     3001 actggatcct tacttcaaac tgaacataga agccaaccat gcgacactgg cacagcatac
     3061 gttccagcat gaactctgtg tggctcgtat caacaacatg ctcggcagca tagacgcgaa
     3121 ccagggcgac ctgatgctcg gctgggatac cgaccagttc ccgacgaaca tttacgatac
     3181 gacacttgca atgctcgaag tcctcaagat gggcggcttc acgaagggcg gcctgaactt
     3241 cgacgccaaa gtcaggagag gctcattcga accggaagac atgttctatg cccatatcgc
     3301 aggcatggat gcattcgcaa aaggcctgaa ggtcgcggca aggataatgg aagacggcaa
     3361 gttcgacaag ttcattgctg acaggtactc tagctataca acgggtatcg gcaaggatat
     3421 agtcgaaaac aaggtcggct tcaaggaact cgagcagtat gtacttgacc tcggcaaagt
     3481 caatatcaag tccggcagac aggaaatgct cgaagtatgg ctcaacagct atatccttga
     3541 agaatgacgg atggtttgcc catactggga caacaagctg taaatgcgca tagaaggaat
     3601 atagataaat atatagaaac tgcgtataga gaaagaatat agggacaggc tgttgcgggg
     3661 ttctgcatca ttactgcgca atgattcaaa accccggaca gccaattttt ttagttcttc
     3721 tgcataggat gatacaggaa ggccgtttgc ttatgctgga ggacaggatg tattctgccg
     3781 gcagtggcgg atggaggaca gtgaagccat acgacaggga cgaggcggtc cggtatgcgc
     3841 ataagtgggc ttatggaagg aatccggcct actacgattt cgaaaaatta ggcggggact
     3901 gcacaaattt cgcttcccag tgcatatatg ccgggagcgg cgtcatgaac catacgccgg
     3961 tctatggctg gtattacatc aactcatata acaggacggc atcctggacc ggcgtcaatt
     4021 atctgtataa attcctcata aataataagg gatcgggtcc ttttgcagaa gaagtggata
     4081 ttggcggtgt catgcccgga gatatagccc agctttcttt ttccgacagc aaacacttcc
     4141 agcactcgcc tgtcatcgtg aaagtttccg atcctccgac catggaaggc atattagtgg
     4201 ccgcccacac attcgatacg gattattacc ggctttctga ctttgaaccg gtacatgtca
     4261 ggttcctgca catagcaggc gttcgggtat gatgatattt tctcattgga tcccgctgca
     4321 actggtcgta atttcttttc ctgccagctt gcgcatcatg ccttcaggat ttttcagcct
     4381 tctttgtgct gttcttcttc gcgggttttt ccggagggac gttttcctct gtaatgacct
     4441 tatccaggtc gagcagcagg atcagcctgt catcgatctt tgcagtgccg ctcaggaact
     4501 tgtccctgac gcttgacagc gctgaaggca caggttcgaa ctggctttcc tcgatcttga
     4561 cgatttcgcg gacactgtcc acgataaggc ctacgacaga gtcatcagaa gtctttgcga
     4621 ttattatcct gttgtcttcg ttgaattcgg ggcaaggctg atggaaacgc ttcctgagat
     4681 tgaatatggt atgaaccttg cctctcagat tggccagtcc ttcgatgtaa ccgggcgtgt
     4741 tcgggatcct gaatatttcg agcattcttt ctattatgct gactttctcg atttcaaggc
     4801 cgaactcgtc accgtcgacc aggaatacca cgtactgctt cacacccact ttgacccctc
     4861 cctgttccgg gtgttatcta tgctgtatat cggctgtcat gtgcggtaac ttgcgtttat
     4921 cttcacataa tcgtatgaaa agtcacatgt ccagacacga tcggaataca cgcctttgtt
     4981 cagctttatc agcaccttta cttcatcctg ctcaagcagc ttccgggctt ccgcctcgtc
     5041 gaagtcgacg gcgcacccgg ctttgcacat gggtatgtct cctatatata tatcgacata
     5101 ttcggggtcg aattcagctc ctgaatagcc gacggcagtc attatcctgc cccagttgac
     5161 atcgttgccg aacatcatcg ttttcaccag gggcgacttt gccaccgcgc atgccgcttt
     5221 atacgcgtcg tccgggtttt cggccccttc gaccgacacc tcgatcagct ttgtggcgcc
     5281 ttccccgtct tttgcgatga gccttgccag gtaagtgcag acatactcca ggcctttgac
     5341 gaatttctcg taatcttctc cttcggtgat gataccgggg ttttccgcca taccgttcgc
     5401 aaatatgacg accatgtcgc acacactggt atctccgtcg acggtgatcc tgttgaagga
     5461 ggatctcacg acagtcctga gcgccttatc gagcagcttt cgggaaatat tggcgtcggt
     5521 agtgatgatg cctatcatgg tcgccatgtt ggggtgtatc atgccggaac ccttggccat
     5581 accgccaatc accacccgcg tatcctgcag ttccagttct at
//
LOCUS       DAOSCC010000162         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9139_length_5600_cov_4.954914, whole genome shotgun sequence.
ACCESSION   DAOSCC010000162 DAOSCC010000000
VERSION     DAOSCC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9139_length_5600_cov_4.954914"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLG72_08690"
     CDS             <1..1340
                     /locus_tag="PLG72_08690"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOJ80910.1"
                     /translation="FDYSNALSFINENEITILSPFIKNAHQMLHNKTGPGSDFTGWVD
                     LPQNYDKAEFARIKEAAGKIRSDSDALVVIGIGGSYLGARAAIEALSHTFHNLLPGDR
                     RGAPEIYFVGNSISSTYIAELFELLEGKEISVNVISKSGTTTEPAIAFRVFREYLESK
                     YGKAGAAKRIYATTDRARGALKKLADEEGYESFVIPDDVGGRYSVLTAVGLLPIAVAG
                     VDIDEMMKGAADAADLYNNDDVAVNGCYQYAAVRNALYRKGKTIEIMVNYEPSMHYFT
                     EWWKQLYGESEGKDHKGIFPAGVDFTTDLHSMGQYIQDGLRSIFETVLSIEKPRKNIT
                     IKEDKDNLDGLNFLAGKDIDYVNRMAMQGTILAHNDGGVPNLVVNIPEMNSYWFGNLV
                     YFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEMERLRARLKK"
     gene            complement(1463..2263)
                     /locus_tag="PLG72_08695"
     CDS             complement(1463..2263)
                     /locus_tag="PLG72_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ80911.1"
                     /translation="MALAIVLLVIAALYAYMKFETTWLKIERLDFSKKGTGLRIMHLT
                     DLHVYMTGIPAERIKKAVKAEDPDIILITGDYINKPVHASEFLRYLQTFAKGRRTIIC
                     LGNHDYKAFGSNTEGLRNFIHEIEALGVEVLVNSNVTVEKDGRKYNIIGFDDLRRGHP
                     DIERAMEGCIPDAPKIAITHNPDLVLGIPGKIVDYMFAGHFHGGQIWMPFNLEFAILR
                     DDQLCRMGIRRGLQRVNGIKVYLNRGLGNGVVPLRFLSRPEILICTIR"
     gene            2421..2783
                     /locus_tag="PLG72_08700"
     CDS             2421..2783
                     /locus_tag="PLG72_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ80912.1"
                     /translation="MKNECCDTCEELKTDPERVNRVRNCMIKDDEAIMLSELFKVLGD
                     QTRIKIIFALSCCEMCVCDIAEAVNMTQSAVSHQLRLMRGMKLVKYRKEGKSVYYSLD
                     DDHILQLFNQGMEHVKHG"
     gene            2776..3555
                     /locus_tag="PLG72_08705"
     CDS             2776..3555
                     /locus_tag="PLG72_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ80913.1"
                     /translation="MDKQNCVADNGVMTIDTATADGLMTEFSLEKILSVPKKYRYETH
                     MHTKEASACAGSTGAEMVRAYYRAGYSGLIVTDHFFNGNSCIPAHLPWAQRVELFCMG
                     YENALREAGKLDFHVFFGWEYNDRGAEFLTYGLGKDFLLAHPDMLSWPIEKYLKVVRE
                     AGGFVSQAHPYREAWYIKEIRVFPEHVDAIEVRNASHTNPKFDERALDLANEYGLYHT
                     GGSDAHFAEYLPGGGMEFDCELFTIGDFIRAVKEGRGKVIP"
     gene            3670..4908
                     /locus_tag="PLG72_08710"
     CDS             3670..4908
                     /locus_tag="PLG72_08710"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOJ80914.1"
                     /translation="MALPLVFLIIFSSICIISLYLGIYTLYTNPRSFTNRIFLALTTA
                     MFIWSFGFAMAISAADISACLFWRRFAAIGWGTFFSILLQFVVSLTGRNHLPDRWWKY
                     VLLYLPAAVCLSVFTYIPGLNPQQFNLVQTPSGWINVPIKNCWDWFYIAYYLGYTLTG
                     IYFLWKWGRTDSSRNVRKQSNIIILAILAALVLGAFTESAANSVFSVRIPQMAPIVML
                     IPVMAVSYAMQKYGFLDIRKNMTDSLLFSEQIRKRVVHYLATSLFVSSVLNVVGMYFI
                     YKDTNLARILVFSGVLIVSGVIFEVIERIEKNDRYKDGSDTRGAFLYTDTRADTELRR
                     VRQHDCLGLHIHFSHCFHWHDKQDHPGLHSFFGHAYTPAGFHIETGTGRPDRCLRPCR
                     QDSPERYSDSPYCLLNVRAI"
     gene            4923..>5600
                     /locus_tag="PLG72_08715"
     CDS             4923..>5600
                     /locus_tag="PLG72_08715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ80915.1"
                     /translation="MDLDSFKNVNDTLGHEGGDELIIEAARKLTDTLRKSDTVSRFGG
                     DEFLIMVKNIASTEDVYKIADKVMNVFRTPFTVCNQEIYISASIGIAVYPFDGKDADT
                     LLRNADIAMYQAKARGKNQYCLCTEDMKEEVVRRHELSNRLYRAIDNNELSLHYQPLV
                     SAKTGEIQCMEALVRWNQPDYGLILPGVFIPLAEQNGLIGRIGEWVLRNACRQTKEWL
                     DMGLPEMH"
BASE COUNT         1466 a         1289 c         1478 g         1367 t
ORIGIN      
        1 gttttgacta ttcaaacgca ttatccttca tcaatgagaa cgagataacg attttgtccc
       61 cttttataaa gaatgctcat caaatgctcc ataacaagac aggacccgga agtgatttca
      121 ccggctgggt ggatcttccc caaaactacg acaaagctga atttgccagg ataaaggagg
      181 ctgccgggaa gataagatcc gattccgatg cacttgtggt gatagggatc ggcggttctt
      241 acctcggtgc aagggcagcg atagaagcac tctcgcatac gttccacaac ctgctgcccg
      301 gagacagacg aggagcgccg gagatctact ttgtaggcaa cagcataagt tcgacttata
      361 tcgcggaact cttcgaattg cttgaaggca aggagatatc cgtcaacgtg atatcgaagt
      421 ccggcacgac caccgagcct gccattgcgt tcagggtctt cagggaatat ctcgaaagca
      481 aatacggcaa ggcaggagcg gcaaaaagga tatatgccac tacggacagg gcaagaggcg
      541 ccctgaagaa actggctgac gaagagggat acgagagctt cgtgatcccg gatgatgtcg
      601 gcgggagata ttcagtgctg acggctgtag gacttctgcc gatagccgtt gcaggcgtcg
      661 atatcgatga gatgatgaag ggcgctgctg atgctgcaga cctttacaat aatgacgatg
      721 tggcagtgaa cgggtgctac cagtatgcgg cagtaaggaa cgcgctttac aggaagggaa
      781 agaccatcga gataatggtc aactatgagc catcgatgca ctattttacg gaatggtgga
      841 aacagcttta cggcgagagc gaaggcaaag accataaggg cattttccct gcaggcgtgg
      901 acttcaccac cgatctgcat tcaatgggcc agtacatcca ggacggcctt cgcagcatat
      961 tcgaaacggt attgagtatc gagaagccca gaaagaacat cacgataaag gaagacaagg
     1021 acaacctgga cggcctgaat ttccttgccg gcaaggatat agactacgtg aacaggatgg
     1081 cgatgcaggg taccattctg gctcataatg acggcggagt gcccaacctc gtcgtgaata
     1141 tacctgaaat gaacagctac tggttcggga acctggtata cttctttgaa aaagcatgcg
     1201 gcatcagcgg atacctgctg ggtgtgaacc cgttcgacca gcccggtgtc gaagcataca
     1261 agaagaacat gttcgcactg ctgggcaagc ccgggtatga gaaggagatg gaacgtctcc
     1321 gggcgcggct gaaaaagtaa tcacggatga ggcccggaca gcatggacaa gtcaggcaga
     1381 taagcgagtt gctcctgtgg ctgagcaaag ttcggccaca ggggcagttt tatttctgac
     1441 acgataatgg aatagcaaaa tgtcatcgga tcgtacagat gagtatttcg ggacgggaca
     1501 ggaagcgcag cgggacgact ccgttgccga ggcccctgtt caggtagacc ttgatgccgt
     1561 taacacgctg cagtcctctc ctgatgccca tcctgcacag ttggtcatcg cgcaggatcg
     1621 caaactccag gttgaagggc atccatatct ggccgccgtg gaagtggccg gcgaacatgt
     1681 aatccactat ttttccggga atgccgagca ccaggtccgg gttgtgcgtt atcgctattt
     1741 tgggggcgtc cgggatgcag ccttccatgg ccctttcgat atccggatgt cctcttctca
     1801 ggtcatcgaa cccgattata ttgtattttc tgccgtcttt ctccactgta acattgctgt
     1861 ttacaagtac ttccacacca agcgcctcta tttcatgtat gaagttccga agcccttccg
     1921 tatttgaccc gaatgccttg taatcgtgat ttccgagaca gattatggtt cttcgccctt
     1981 tggcgaatgt ttgaagatac ctgaggaact ccgatgcatg cacaggcttg tttatatagt
     2041 ctcctgtaat gagaatgata tcagggtcct cggctttgac cgcttttttg atgcgctccg
     2101 ccgggatccc cgtcatgtag acatgcagat ccgtcaggtg catgatgcga agcccggttc
     2161 cttttttgga aaaatcgagc ctttctatct tcagccatgt tgtctcgaac ttcatatatg
     2221 catataacgc cgctatcacc aataatacta tagccagagc catgcagaac tctcccggta
     2281 ccagctttta tatataaata taccaattgc cgtcaaagtc agcaacatcc attttggctg
     2341 ctatttgact aatactggtt ttaaaaatat aataaaatta tcgggctgga atccgaaatt
     2401 tattccggat ggcgatcggt atgaaaaacg aatgctgtga cacctgcgaa gaactgaaga
     2461 ctgatcctga acgtgtcaac agggtccgga actgcatgat aaaagacgac gaggctatca
     2521 tgctttccga attgttcaag gtgctgggtg atcagacaag gatcaagatc atctttgcat
     2581 tgtcctgctg cgaaatgtgt gtatgtgata tcgcggaagc ggtaaatatg acacagtctg
     2641 ccgtatcgca ccaactcagg ctcatgaggg gcatgaagct ggtcaaatac aggaaggaag
     2701 gcaaatccgt atattattca ctggatgatg atcatatact gcagcttttc aaccagggta
     2761 tggagcatgt gaaacatgga taaacaaaat tgcgttgctg ataatggggt catgacgatc
     2821 gatactgcga cagcggatgg attgatgacg gaattttcac tggagaagat actttccgta
     2881 ccaaagaaat acagatacga aacccacatg catacaaaag aagcaagtgc atgtgccggg
     2941 agcaccggcg cggaaatggt gcgggcctat tatcgggcag gctactcggg actcatagtc
     3001 accgatcact tcttcaacgg aaactcgtgc atacctgctc atctgccatg ggcgcagcgg
     3061 gtggagctat tttgcatggg ctacgaaaat gcgctgcgtg aagcaggcaa actggacttc
     3121 catgtgtttt tcggatggga atacaatgac agaggggctg aattcctgac atacggactg
     3181 ggcaaggatt tcctcctggc acaccctgac atgctgtcat ggccgatcga gaagtatctc
     3241 aaagtcgtgc gggaagccgg cggattcgtt tcacaggccc atccgtaccg ggaagcatgg
     3301 tacataaaag agatccgtgt ttttcccgag catgtggacg cgatagaagt ccgcaatgca
     3361 agccatacga atccgaagtt cgacgagcgg gcgctggacc tggccaacga atacgggctt
     3421 tatcacaccg gcggttccga cgcgcacttc gcggagtatt tgcccggcgg aggcatggaa
     3481 ttcgactgtg agctttttac catcggggat ttcatacgag ccgttaagga aggaagggga
     3541 aaagtgatcc cctgatacaa gcccgggctt gtcatatgta atacaaggtt agaggaaacg
     3601 ttatccagtt aaaagtaaat accgatatat aaagcagata tatgtatatc ttgcgggagt
     3661 gacagcgtga tggccttgcc tcttgtgttt ttgatcatct tcagttcgat ttgcattata
     3721 agtctatatc tgggcatcta cacactttat acaaatccaa gatctttcac gaaccggatc
     3781 ttccttgcac tgacgactgc gatgttcatt tggtcgtttg gttttgccat ggccatctcg
     3841 gcggccgata tatccgcatg cctgttctgg agaaggttcg cagccatcgg atgggggact
     3901 tttttcagta tcctgctcca gttcgtggtt tcgctgaccg gcaggaacca tctgcctgac
     3961 aggtggtgga aatatgtgct gctttatctt cccgcagcag tatgcctttc cgtatttaca
     4021 tatatcccgg ggctcaatcc gcagcaattc aacctggtac agactccttc cggctggatc
     4081 aacgtcccga tcaaaaactg ctgggattgg ttctatatag cgtattacct gggttatacc
     4141 ctgaccggca tatacttctt atggaaatgg ggaagaacgg acagcagcag gaacgtgaga
     4201 aagcagtcca atataatcat cctggcaatc ctggccgctc ttgtgctggg agcttttacc
     4261 gagagtgctg ccaacagcgt attttctgtc aggatacctc agatggcccc gatcgtcatg
     4321 ctgatcccgg tcatggcggt cagttatgct atgcagaagt atggttttct tgatataagg
     4381 aagaacatga cggactcact gctgttcagc gaacagatca ggaagagagt ggtccattac
     4441 ctggcgactt ccctttttgt ctcaagtgtg ctcaatgtag taggcatgta ttttatctac
     4501 aaggatacga acctggcccg gatcctggtt tttagcggcg ttttgatagt aagcggcgtc
     4561 attttcgagg taatcgaaag gatcgagaaa aacgacaggt acaaggatgg atctgataca
     4621 cgcggtgcat ttctttatac tgatacccgt gctgacactg aattacgtag agtacggcag
     4681 catgactgtc tgggcctgca cattcatttt tctcattgtt tccattggca tgataagcag
     4741 gatcatccag gtctccatag cttcttcggt catgcttaca cacctgctgg ttttcatatt
     4801 gaaaccggaa ctggtcgtcc ggatcgatgc ctcagaccat gtcggcagga tagccctgag
     4861 cgctatagcg acagtccgta ctgcttgctg aacgtacgtg cgatataatc gcagtgattt
     4921 tcatggatct tgactcattt aagaatgtga acgatacgct tggacatgaa ggcggagatg
     4981 aacttattat tgaagccgcc cggaagctga ccgatacgct gcgcaaatcc gatacggtgt
     5041 cccgtttcgg cggagatgaa ttcctgatca tggtcaaaaa catagcaagc acagaggatg
     5101 tctataaaat tgccgacaag gtgatgaatg ttttcaggac tccatttact gtgtgcaacc
     5161 aggagatata catatctgca agtattggca tagcagttta tcctttcgac gggaaagatg
     5221 ccgataccct gttaaggaat gcggatattg ccatgtacca ggcgaaagcc agggggaaaa
     5281 accagtattg cctgtgtacc gaggatatga aggaggaagt cgtacgcagg cacgaacttt
     5341 ccaacaggct gtacagggca atagacaaca acgaactgag cctgcattac cagccgctgg
     5401 tatcggcgaa aactggcgag atccagtgta tggaggcttt ggtacggtgg aaccagcctg
     5461 attacggact catcttaccg ggagtcttca taccgctggc agagcagaac ggcctgatag
     5521 gccggatcgg cgaatgggtg ctgagaaatg catgcaggca gacgaaagaa tggcttgaca
     5581 tgggcttgcc tgaaatgcat
//
LOCUS       DAOSCC010000163         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9259_length_5535_cov_3.840146, whole genome shotgun sequence.
ACCESSION   DAOSCC010000163 DAOSCC010000000
VERSION     DAOSCC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9259_length_5535_cov_3.840146"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLG72_08720"
     CDS             <1..858
                     /locus_tag="PLG72_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ80916.1"
                     /translation="PEAPTGWVAAVQWAADQTAKRLGLNYKLLVSADENTQANHIDEL
                     IQLGCETIVLMPHNDTLGTAAQKIKDAGITLINFDRTLGATEPDYYVAGDNYAMGVLG
                     AEYIAEKLGGKGKVVIANIPAYGEIFTQRVNGFRDTIKEKYPEIEILQEYGAENGAPE
                     TGLRIMTDILTAYPSGIDGIYSTDDELSIGLIQAISEAGRMGDIKVITGGGGSQAYFK
                     VMEQYPEMHISSQTYDPYMMITAVEIAAGLQEGKTYESKTIIPPKNLDRANYQAWLDE
                     NHITADAPY"
     gene            1152..2663
                     /locus_tag="PLG72_08725"
     CDS             1152..2663
                     /locus_tag="PLG72_08725"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ80917.1"
                     /translation="MEKFSLQMKDIVKSYNGVTVLKGVSLNVRQGEIHALLGENGAGK
                     TTLMNILGGVIPKDSGEIHLFGEPAKISSPADSQALGIAFIHQELNVVNDLRVYENLF
                     LGRELHKKSGILDAKRMCEETAKIFSKMNVTIDPRAMMRDLETSYKQIVEIAKSLLMD
                     ARLIIMDEPTTSLTQKEIDNVFNIIRGLTKDHDVTVIFISHKLGEVVDFCDSYTVLRN
                     GENVSSGSITQPDGSKINQADLARMMVGKDVLNQQVYEEHEIGRTLLEVKDMTVDNRV
                     KGVSFSLKEGEILGITGLLGDGKDEMVRALFGDLHHTGTVLKDGKQLSIRHPSHAKKL
                     GIGYLPPNRKENAIIKDMSVQDNINITALEKCRRGISLSRRKERDIAREFIEKLRIKT
                     DSLICKITSLSGGNQQKCVLSKWLNARPDVMIMSNPTQGVDVGAKNEIYTEIMKLAKQ
                     KIGIIVTSGEAQEIIKLCDRCLVMYHGELRGELSRDQLTEENIMILSTGGSLN"
     gene            2711..3688
                     /locus_tag="PLG72_08730"
     CDS             2711..3688
                     /locus_tag="PLG72_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ80918.1"
                     /translation="MQNTVSKQRFGSLKKVVSSNSIVLVVIAMIIAGTFAEGSQFLSF
                     QNFMNILRNHAVIGIIALGMSFVIISGNIDLSVGAQMVAVAAITVSFVNSTGNIVMGL
                     ILGILAGCAMSTVTGIIVTKGRVPSFIVTLGMQYIYRSLCMFYMQGGGFYLDKTKAPF
                     YTQISNYMLFGKIPMPVVYYALMFLLFWFISRYTKLGRHIYAVGSNEKATKLSGINTD
                     KIKILAFTLMGLAISVAAIVESSRMGSINSTSSGNAYELNAIAMTVIGGISMEGGKGK
                     ISGALWGMLILGIINNMITLLGVNVYLINAFKGVIIIAAVLLQRKDREL"
     gene            3879..4739
                     /locus_tag="PLG72_08735"
     CDS             3879..4739
                     /locus_tag="PLG72_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ80919.1"
                     /translation="MKIGIDSYCFHRLFGEVYDFQEKPERFWTVEEFLEFAKKLDVDG
                     VSLETCFLPSLDDGYLKELGAKIKEYGFDTVFAWGHPNGLERGLNPAAFEEMKSLIPK
                     TRLLNTDVMRITGSAFDWRHENHRDHIERLVPMYKEAVKIAEANGVKIAAENHIDFTS
                     DEMLEIIERVDSPNFGINFDTGNFLRLLDDPVAGMEKLAPYVFSTHIKDLKIHPTAKP
                     TDWYFFCGVPVGFGMVDNLKLAELLHKAGYKGFLAVEIDAPAPEWLGMEEEAVAVSVK
                     NLKKIAAHFK"
     gene            4879..>5535
                     /locus_tag="PLG72_08740"
     CDS             4879..>5535
                     /locus_tag="PLG72_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ80920.1"
                     /translation="MGKKKINIAMIGGGFMGKAHSNAWRRVNEFFDAEFEPVLKVIAG
                     NRTRLEDFAERWGYEEVTYDWRTVVERDDIDVIDIGTPTYEHKEMAIAAARAGKHIVC
                     EKPFTLTYADAKEMVDAVEKAGVVHYLNHNYRRAPAVAYAKQLIEEGRLGTIYHWRGA
                     YLQDWIADPSFPLSWQLQKEKAGGGPLFDLSSHAIDLARYLIGEPEAVTAVNKTFIKK
                     R"
BASE COUNT         1599 a         1301 c         1458 g         1177 t
ORIGIN      
        1 cctgaagctc cgacaggctg ggtagcagca gtacagtggg cagctgacca gactgcaaag
       61 agacttggtc tcaactacaa gctccttgtt tcagcagatg aaaacaccca ggcaaaccac
      121 atcgatgaat tgatccagct tggctgcgaa accatcgttc tgatgccgca caacgatact
      181 ctcggtactg cagcacagaa gatcaaggac gcaggcatta ccctgatcaa cttcgaccgt
      241 actctcggcg caacagagcc tgattactat gtagcaggcg acaactacgc aatgggagtc
      301 ctgggcgctg aatacatcgc agaaaaactg ggcggcaaag gtaaagtagt tattgccaac
      361 atccctgctt acggcgaaat attcacacag cgtgttaacg ggttcagaga cactataaag
      421 gaaaagtatc ctgaaatcga gatcctccag gagtatggcg cagagaacgg cgctcctgaa
      481 acaggcctgc gcatcatgac ggatatactg accgcatatc cgagcggtat cgacggcatc
      541 tactccaccg acgacgagct ttcgatcggc ctcatccagg ctataagcga agccggccgt
      601 atgggcgaca tcaaggtcat caccggcggc ggcggatcac aggcttactt caaggttatg
      661 gaacagtatc ctgaaatgca tatctcttca cagacctacg atccttacat gatgatcacg
      721 gctgttgaga ttgcagcagg cctccaggaa ggaaaaactt atgaaagcaa gaccataatc
      781 cctccgaaga accttgaccg cgccaactat caggcatggc ttgatgaaaa ccatataaca
      841 gctgacgctc cgtactaata gtgcaggtgt tgcgggaacc gggatccgcg gaaggacccg
      901 gccggaaaaa gctcatgtaa ctgaaggcat tatatgggag acggcttagg ccgtctcctg
      961 tataaacgta ccgcgttaga taaagcatgc aaaaagttat ttaatgatcc aggtcacagt
     1021 gtcgctgtga tttcggaaga tacgaggcaa taaacatgac ccgcctcgtt atggacgctg
     1081 ccgaggaaag agaatccttc cgataacgga gattttcttt gaaccaaggt gttataagtg
     1141 aggagaccag catggaaaaa ttctcgttgc agatgaaaga cattgttaaa tcgtacaacg
     1201 gagttacggt tttaaaaggt gtcagtctca atgtgcggca gggcgaaatc cacgcactgt
     1261 taggcgaaaa cggggcaggc aaaacaacgc tgatgaatat acttggagga gtcataccca
     1321 aggattcggg agaaatacac ctgttcgggg aaccggcaaa gatcagttcg ccggccgact
     1381 cccaggcgct gggcatcgct tttatccacc aggaactgaa tgtcgtcaac gacctcaggg
     1441 tctatgagaa cctgttcctc ggccgagaat tgcacaagaa gtccgggata ctcgatgcaa
     1501 aacgaatgtg cgaggaaacg gcgaaaatat tcagcaaaat gaatgttacc atcgatccca
     1561 gggcaatgat gagggatctc gaaacgtcgt acaaacagat agtagagata gcaaaatcat
     1621 tattgatgga tgcaaggctg atcataatgg atgagccgac cacatcactg acgcagaaag
     1681 agatagacaa cgtcttcaat ataatccgtg gtcttacgaa ggaccatgat gtcacagtca
     1741 tattcatttc acacaagctt ggggaagtcg tggatttctg tgacagctat accgtcctgc
     1801 gaaacgggga gaatgtctct tcaggcagca taacccagcc ggacggctca aagataaacc
     1861 aggccgatct ggcaaggatg atggtcggaa aagacgtact gaaccagcag gtatatgagg
     1921 aacacgaaat aggcaggaca ttgctggaag taaaagatat gacggtagac aaccgtgtaa
     1981 agggagtaag cttttcactg aaggaaggag agatccttgg gatcacgggg ctccttggcg
     2041 acgggaagga tgaaatggta cgcgcactgt tcggcgacct ccatcatacc ggaacggtgc
     2101 tcaaagacgg aaaacagctg tccatacgcc atccgtcaca tgccaaaaag ctcgggatcg
     2161 gctacctgcc gcctaaccgt aaggaaaatg cgatcatcaa ggacatgtcc gtccaggaca
     2221 acataaacat cacggcactg gaaaaatgcc gccgcgggat aagcctcagc cgcagaaagg
     2281 aaagggacat tgcccgggag tttattgaaa agcttcggat aaaaacagac agtttgattt
     2341 gcaagataac aagcttgtca ggcgggaacc agcaaaagtg cgtcctttcg aaatggctca
     2401 atgccaggcc ggacgtcatg ataatgtcca accctacaca gggcgtggac gtgggcgcga
     2461 agaacgagat ctataccgag atcatgaagc ttgccaagca aaaaataggc attatcgtaa
     2521 cttcaggcga agcacaggaa ataatcaagc tgtgcgaccg ctgtctcgtt atgtaccatg
     2581 gagaactgcg cggggaactg agccgcgatc agttgaccga ggagaacatc atgatactgt
     2641 ctacgggagg cagcctgaat taataatctg caggggacag cttaaactaa gatttgggga
     2701 gtagggaaaa atgcagaaca cagtttccaa acagagattc ggttctctga aaaaggttgt
     2761 gagcagcaac agcatcgtcc tggtagttat cgcgatgata atcgccggta cgtttgccga
     2821 aggctctcag ttcctgtcgt tccagaactt tatgaacatc ctgcgcaacc atgcggtcat
     2881 aggcataatt gcgctgggaa tgtcgttcgt aatcatttca ggcaatatcg acctgtctgt
     2941 cggcgcccag atggtggcag tcgcagccat tacggtttcg tttgtcaaca gcaccggcaa
     3001 tatagtaatg ggtttgatcc ttggcatcct ggccggctgc gccatgtcca cggtcaccgg
     3061 catcatagtt acgaaaggcc gtgttccttc atttatagtg acgctgggca tgcagtatat
     3121 ataccgttca ttgtgcatgt tctacatgca gggcggcggg ttctatctcg ataaaaccaa
     3181 ggctccgttc tacacgcaga tatcaaacta catgctgttc ggcaagatac ccatgcctgt
     3241 agtttattac gcgctgatgt tcctgctgtt ctggttcata tccaggtaca cgaagcttgg
     3301 acggcatata tatgcagtcg gttccaacga gaaagccacg aagctttcag gtatcaatac
     3361 cgataaaata aagatcctgg catttaccct catgggtctg gcaatatcgg tggccgccat
     3421 agttgagagt tcaaggatgg gctccatcaa ctcaacttca tccggtaacg catatgaact
     3481 gaatgcgatc gcaatgaccg tcataggcgg catctcgatg gaaggcggta agggcaagat
     3541 atccggggct ctctggggca tgctgatcct tggaattatc aacaacatga tcacattgct
     3601 cggcgtgaac gtatacctga taaacgcatt caagggcgtg atcatcatcg ctgccgtgct
     3661 ccttcagagg aaagacaggg aactctgatc ataaagaaac gacagatatc ctccgcttat
     3721 acgggcggcg gatatctgtt tgcgtttcat tcgtaaacca gttcgcatct catacgcaag
     3781 ccaaagagtt aatcctgact gtagccaaat tatcgtcggg ctgtatgccg gatgataatt
     3841 tggaaaatat aaaaaataaa acaacaggag ggttatatat gaagatagga atcgacagtt
     3901 attgttttca caggcttttc ggcgaagtgt acgattttca ggagaagccc gaaaggttct
     3961 ggaccgttga agagttcctg gaatttgcaa aaaaactcga tgtagacggc gtatcgcttg
     4021 aaacctgttt cctgcccagc cttgatgacg gctatctgaa agagcttggc gcaaagataa
     4081 aggaatacgg tttcgacaca gtatttgcgt ggggccatcc gaacggcctc gaacgcggac
     4141 tgaacccggc agcgttcgaa gaaatgaaat cgctcatacc aaagacacgt cttctcaaca
     4201 ccgacgtaat gcgcattacc gggtccgcat tcgactggcg gcatgaaaac caccgcgacc
     4261 acatcgaaag gctcgtaccc atgtacaagg aagcggtaaa aattgctgaa gccaatggtg
     4321 tcaagattgc agcggagaac cacatcgatt ttacgtcaga tgaaatgctt gaaatcatcg
     4381 agagagtgga ttcccccaat ttcggcataa actttgacac gggcaatttc ctgcgtttgc
     4441 tggacgatcc ggttgccgga atggagaaac tcgctcccta tgtattctca acccacatta
     4501 aagacctgaa aatacacccg acggcaaaac ccaccgattg gtacttcttc tgcggcgtgc
     4561 ccgtaggatt cggaatggtc gacaacctga agctggccga actgctccac aaggcaggct
     4621 ataaaggctt cctcgcagtg gagatcgacg caccggctcc ggaatggctg ggcatggagg
     4681 aagaggctgt cgcagtcagc gttaagaacc tgaagaagat cgctgctcat ttcaaataaa
     4741 ctgcggcagc atgacgacaa acagctgaca ggatgcacac tgtcgggaga tccgcaattt
     4801 cagcggagat ccgtgttgtt tcgggaggat gcatcctgcc ggacagttta ggatttggaa
     4861 agcgaaagga ggcttaacat gggaaagaag aagataaaca tcgccatgat aggcggcggc
     4921 ttcatgggta aggcgcacag caatgcatgg cgcagagtga atgaattttt cgacgcggaa
     4981 ttcgagcctg tgctgaaagt catagcaggc aacaggacaa ggcttgaaga tttcgcagaa
     5041 agatgggggt atgaagaggt cacctacgac tggcgtaccg ttgtggagcg cgatgacatc
     5101 gatgtaatcg acatcgggac tcccacttat gaacataagg aaatggcaat tgccgcagcc
     5161 agggcaggca agcacatagt atgcgaaaag ccattcacgc tgacgtacgc tgatgctaaa
     5221 gaaatggtag acgcagtcga gaaagccggc gttgttcatt atctgaacca caactaccgc
     5281 agggctcctg ctgttgcata tgccaagcaa ttgatcgaag aaggcaggct cggtacgatc
     5341 tatcactggc gcggggctta cctgcaggac tggatcgccg atcccagctt cccgctgtca
     5401 tggcagctgc agaaggaaaa agcgggcggc gggccgctgt ttgacctgtc atcgcacgca
     5461 atcgaccttg caaggtattt aataggagag cccgaggctg taacggcagt gaacaaaacg
     5521 ttcattaaaa aacgt
//
LOCUS       DAOSCC010000164         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9306_length_5517_cov_4.267301, whole genome shotgun sequence.
ACCESSION   DAOSCC010000164 DAOSCC010000000
VERSION     DAOSCC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_5517_cov_4.267301"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..979
                     /gene="raiA"
                     /locus_tag="PLG72_08745"
     CDS             440..979
                     /gene="raiA"
                     /locus_tag="PLG72_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ80921.1"
                     /translation="MNLKITGKNFEVTDALKEKVNKKLGKLDKFFKPDTEAQVTMSVQ
                     RNRHILEVTIVTGGITLRAEVTGDDMYSCIDKAQDILERQIRKHKTRLAKRLHEEVLD
                     QPGAFRPDIDDVEEEKEFRVVRTKRFAVKPMPLDEAILQMNLLEHEFFVFSNAETNQV
                     NVVYKRKDGNYGLIEPEFD"
     gene            1212..2612
                     /gene="lpdA"
                     /locus_tag="PLG72_08750"
     CDS             1212..2612
                     /gene="lpdA"
                     /locus_tag="PLG72_08750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOJ80922.1"
                     /translation="MFGLIYDLIVIGGGPAGYHAAELAGKSGLNTLLAEKRHIGGVCL
                     NEGCIPSKTLLYSAKLFYGAVHGEKYGITVENAVLDHKKVIDRKNKVVRTLAAGVKAR
                     LRQSNVTVFEGAAVIAGMRAGADGETVFDVKAGDDMYTGRRILIATGSVPVLPGIPGI
                     KDGLDSGRVMTNREIFDLKEVPDSLAVIGGGIVGLEMASYFSAAGSHVMVLEMLDHIA
                     GSVDREISDILQRNLSKRGVEFLLNCRATGINDGGVVYTSEPDGGEPKFVKADKVLVS
                     IGRRPATEGLGLENIGVAVEKGRIITDLHGRTNVPGVYAAGDVNGVSMLAHTAYREAE
                     ACINDMLGIDDEVRYDAIPSVIYTDPEAAGVGETEASAAEKGLDFETAKLPMTYSGRY
                     VAENERGDGICKILIDRRLGTVIGVHMIGSYSSEIIYGAAMMIEKRMRVDDLKKVVFP
                     HPTISEIIREAITEPK"
     gene            complement(2705..4549)
                     /locus_tag="PLG72_08755"
     CDS             complement(2705..4549)
                     /locus_tag="PLG72_08755"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ80923.1"
                     /translation="MATRLRKFSTSPVTKAIAFILAVLIPTGLVVLALFANFSYLDTE
                     CLFVEDYFDSRDFANRMRKAAAEVSDTLYSRVPPENVPYYYYVSDGKKEWSNVGKQDK
                     SFFEQFEPAFCSLENGQWSFGRSTDFTASGIYTDRLKYYAVVDKSNTIYVAYPSETSR
                     AWEQKWQKDRETFVPMFMAAAAGIVLLLVLAAYLAAVTGRKPHDRDLHPGKTDSIFSE
                     ILIAVLLLTALAWYAIIEPAFDENPIDTGELGTYHIVVLVLVGIITSAAATICGTILL
                     TLIRKLKAGSLVKHSIIYKIFYRIYDLAVSLFDGRAFSKYPLTKSLFYRQLIFIILSF
                     VFVFLTFLFIALETYAALIPPVLEMVMIYWYIKGNNRTYMDINRGFDESLEEQMKSER
                     MKVALITNVSHDLKTPLTSIISYIDLLSKEEGLPETAKDYVKILGEKAGRLNNMVSDL
                     FDLAKSTSGNMPVDIEKLDLKKLIEQTLADMGDRIEKSGLAFRVRLPEKPMQINADGK
                     KLYRVFQNVIDNALKYSLQGTRVYVALEENEGMAVATVKNTAGYEMDFTADEILQRFM
                     RGDKSRTTEGSGLGLSIAESYTKLCGGRFKVDIDGDLFKVTISFNLVS"
     gene            complement(4524..5261)
                     /locus_tag="PLG72_08760"
     CDS             complement(4524..5261)
                     /locus_tag="PLG72_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ80924.1"
                     /translation="MDNARISSGDTRASNARILVVDDDREIAMAVEKLLTREGYEVIR
                     AYDGMQALEALAENQIHLILLDIMMPKLDGLSATMKIRESRNIPIIIISAKSEDSDKI
                     LGLSMGADDYVTKPFNPQELVARVKSQLRRYMHLGDIANGSRSSQLRVGGLCLDTDAK
                     QLTVDGEPVRLTAKEYKILELLMSNPGVVFSAEQIYEAVWKDVPYSVENTVMVHIRRI
                     REKIEINPKEPKYLKVVWGIGYKIEKV"
BASE COUNT         1375 a         1285 c         1472 g         1385 t
ORIGIN      
        1 atcgatggag cttaaagcaa ttgaatatgc cttaagacgg gatgtctgtg gataacatca
       61 agccctgccg gtacctggcc agggctttgt ttgtttggca gaggatacct gttttccggc
      121 tttgagggga tcaatgatcc acagcgtcag gatttgcacc gcggtataat ccacagcccg
      181 ggaaagcctg aaaagcagtt atgcacatag ttatccacat tatccacatg gagatattca
      241 caaaacacct gttttgtgaa tatccggccg gggatgttga taatctgtga aatgacgcgt
      301 ttgagcatac ttaccgtgag gtatatataa atgtaaatat accaataatt ttggaaggaa
      361 ttatgataat cattgttgaa taaaaaatat atggcggtct gataactgac aaataccgat
      421 tatgaaagga gcagtgacta tgaatctgaa gatcacggga aagaacttcg aggtaacaga
      481 tgctctgaag gaaaaagtaa ataaaaagct cggaaagctg gacaagttct tcaaacctga
      541 cactgaagca caggttacga tgagtgtaca gagaaacagg cacatacttg aagttacgat
      601 agttaccggc gggatcactc tgcgtgcaga agtgaccgga gacgatatgt attcgtgcat
      661 agacaaggca caggacatcc tcgaaaggca gatcaggaaa cacaagacca ggcttgcaaa
      721 gaggctgcac gaagaagtgc tggaccagcc cggcgcattc cgcccggaca ttgatgatgt
      781 ggaggaagag aaggaattca gggtcgtgcg tacgaaaagg ttcgccgtaa agccgatgcc
      841 cttggatgaa gccatattgc agatgaacct gctcgagcat gagttcttcg tgttctccaa
      901 cgcggaaacc aaccaggtca atgtcgtcta caagcgcaag gacgggaatt acggcctgat
      961 cgagccagaa ttcgactgat gtcgggacgg cccggggagc cgggacagtt tcgtccacat
     1021 gccggcatcc gtatggttcg gctggagtgt ataaagtata aatgatgcag tgaccgacaa
     1081 cgggtaaacc ggataaaata gtgacgccgc ggcgtcacta ttagttttcg caggccaggc
     1141 cgactgcctg gacggcggga caggcctgga cccggttgac ggatcgggga tgtgcgattt
     1201 attgataagg agtgttcggt ttgatatacg atctgatagt aatagggggc ggaccggcgg
     1261 gctaccatgc cgcggaactt gcgggaaaat cgggactgaa tacgctgctg gctgagaaaa
     1321 ggcatatagg gggagtttgc ctgaatgaag gttgcatacc ttcaaaaact ttgctgtaca
     1381 gcgccaagct tttctacggc gccgtccacg gggaaaaata cggaataacc gttgaaaatg
     1441 ccgtattgga tcataaaaag gtcattgaca ggaaaaacaa ggtcgtacgg acgcttgccg
     1501 ccggtgtgaa ggccaggctc aggcaaagca atgtgacagt gttcgagggc gccgccgtga
     1561 tagccgggat gagggccgga gctgacggtg aaacggtttt tgatgtaaaa gcaggcgatg
     1621 acatgtatac cggccgccgc atcctgatcg cgacgggatc ggtacctgtg ctgcctggga
     1681 tacccggtat aaaggatggg ctggattccg gccgggtaat gacgaacagg gagatattcg
     1741 acctgaaaga ggtgccggat tcgcttgcgg taataggcgg aggcatcgtc ggccttgaga
     1801 tggcttcata tttcagcgct gccggcagcc atgtcatggt gctggaaatg ctggatcaca
     1861 ttgcaggctc tgtcgacagg gaaatttctg atatactgca gaggaatctt tcaaaaaggg
     1921 gcgtcgaatt cctgctgaac tgcagggcaa cgggaatcaa tgacggcgga gtggtctata
     1981 catcagagcc tgacggcggc gagccaaaat tcgtgaaagc ggataaggtg ctcgtgagta
     2041 tcggaaggag gccggcgact gaaggccttg ggcttgagaa cataggcgtt gccgtggaga
     2101 aaggccggat catcaccgac ctgcatggaa ggacaaatgt ccctggtgta tatgcggcag
     2161 gggatgtaaa cggcgtttcg atgcttgcac atacggctta ccgggaggca gaggcctgta
     2221 tcaacgacat gctgggaata gatgatgagg tcaggtatga tgcaatacct tcagtcattt
     2281 ataccgatcc cgaggcagcc ggcgtaggtg agacggaagc ttcggccgca gaaaaggggt
     2341 tggatttcga gacggctaag ctgcctatga cctacagcgg aagatatgtc gctgaaaatg
     2401 agagaggaga cggcatttgc aagatcctta tcgacaggcg gctggggacc gtgataggcg
     2461 tccatatgat cgggagctat tcgtcggaga tcatatatgg ggcggccatg atgatagaaa
     2521 aaaggatgcg ggtggatgac ctgaagaaag tggtattccc gcatccgaca atatcggaga
     2581 tcatcaggga agcgataacc gagcctaaat aaccggcgga ccggaagtgc ggcatccctg
     2641 cacttccggt cccgggtcaa atttatgagg agttatgccg gcatggtcag gccggcataa
     2701 tttattaact tacgaggtta aagcttatcg taactttgaa aaggtcgccg tctatatcca
     2761 ctttgaacct tccgccgcac agcttcgtat agctttccgc tatcgaaagg ccaaggccgc
     2821 ttccttccgt tgtgcgcgat ttgtcgcccc tcatgaaccg ctgcagtatt tcatccgcgg
     2881 tgaaatccat ttcatatccg gccgtatttt ttaccgtcgc gacggccatg ccttcgtttt
     2941 cctcaagggc gacatatacc ctggtaccct ggagcgagta tttcagcgcg ttgtcgatca
     3001 cgttctggaa aaccctgtag agttttttgc cgtcagcatt aatttgcatt ggtttttccg
     3061 gcagcctgac cctgaacgca agccctgatt tttcgatcct gtcgcccata tccgccagcg
     3121 tctgttctat cagtttcttc agatccagtt tttcgatgtc gaccggcatg ttgccgctgg
     3181 tacttttggc caggtcgaaa aggtctgata ccatattgtt gagcctgcct gctttttcac
     3241 ccagtatttt tacatagtcc ttcgctgttt ccggaagacc ttcttctttg gacagcaggt
     3301 cgatgtagct tatgatcgaa gtcagcggtg tcttgaggtc gtgtgataca ttggttatga
     3361 gcgccacttt catcctctcg gatttcatct gttcttcgag gctttcatcg aagcccctgt
     3421 tgatatccat gtatgtcctg ttgttccctt ttatatacca gtagatcatt accatttcca
     3481 gtaccggcgg aataagcgca gcataggttt ccagcgcgat gaacaggaaa gtgaggaata
     3541 cgaatacaaa gctcaggatt ataaatatga gctgcctgta aaaaagggat ttcgtcaggg
     3601 gatactttga aaacgccctg ccgtcgaaca ggctcacagc aagatcatag atacggtaaa
     3661 agatcttgta aatgatactg tgttttacca ggctgccggc tttcagcttg cggatgagcg
     3721 taagcaggat cgttccgcat attgtggctg cagcgctcgt tatgatcccg acgagcacca
     3781 gcaccactat atggtatgtg cctaattctc ccgtatcgat cgggttttca tcgaacgcag
     3841 gttcaattat cgcgtaccag gccagtgcgg ttaaaagcaa tacggcaata agtatttctg
     3901 aaaatatgga atctgtctta ccgggatgca gatccctgtc atggggcttt ctgcctgtca
     3961 ctgcagccag gtatgccgca aggaccagga gtaatacgat tcctgctgct gcagccatga
     4021 acattggcac gaaagtctct ctgtccttct gccatttttg ctcccatgct ctcgaagttt
     4081 cagaagggta tgctacgtat atggtattgc ttttgtcaac aacggcatag tacttcaatc
     4141 tgtcggtata tataccggaa gctgtaaagt cagtacttct tccgaatgac cattgtccgt
     4201 tttccagcga gcagaatgcc ggttcgaact gttcaaaaaa gcttttatcc tgtttaccta
     4261 cgttggacca ttcttttttt ccatcgctta cataatagta ataagggaca ttttccggcg
     4321 gcactcttga ataaagagtg tcgctcactt ctgccgctgc tttcctcatc ctgttggcaa
     4381 aatccctgct gtcgaaatag tcttcgacaa acaggcattc cgtatccagg taactgaagt
     4441 tcgcgaaaag ggccagcact accaggccgg ttggtataag caccgccagg atgaatgcga
     4501 tcgcctttgt caccgggctt gtgctaaact ttctcaatct tgtagccaat tccccacacc
     4561 accttcaaat attttggctc cttagggttg atttcgattt tttcacggat gcgccttatg
     4621 tggaccatga ccgtgttttc cacggagtaa gggacgtcct tccagacagc ctcatagatc
     4681 tgctccgcgg aaaatacgac gcccgggttc gacatcagga gttcaagtat cttatactct
     4741 tttgcagtaa gcctgacagg ttcgccgtcc acggtaagct gtttcgcgtc agtatcgagg
     4801 caaagcccgc cgaccctgag ttgggaagac cttgacccgt tggctatgtc gccgagatgc
     4861 atgtagcgcc tcagctggct ttttacgcgc gcgacgagct cctgcggatt gaatggtttt
     4921 gtcacataat cgtccgcccc catcgacagc ccgaggatct tgtcgctgtc ttcggatttg
     4981 gcggagatta ttataatggg tatgttcctg ctctcgcgta tcttcatcgt ggcagaaagc
     5041 ccgtcaagtt tcggcatcat gatgtcaagc aggatcaggt gtatctggtt ttcagccagc
     5101 gcttccagcg cctgcatccc gtcatatgcg cggatgactt cgtagccttc gcgcgtcagg
     5161 agcttttcga cagccatggc gatttccctg tcatcgtcga ctacgagtat cctggcgttg
     5221 cttgcccggg tatccccgga ggatatcctt gcgttatcca actgcgcatc ccccttcagc
     5281 ctggatagat tttacggcag gaatcttacg accggcctca taaaattctt aaaaatatct
     5341 taacaactca gcgatccgga taaagtgagc gaatcagcat ttgtccgatg tatgccataa
     5401 ctgtattata atacacattc cggttttttt ggacaaacgg gcgctatgcc gcccggaacg
     5461 ttatttatgc cggtattgag gggaaaataa aacaggcatt tagtgaaaat gcccggt
//
LOCUS       DAOSCC010000165         5470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9404_length_5470_cov_4.812004, whole genome shotgun sequence.
ACCESSION   DAOSCC010000165 DAOSCC010000000
VERSION     DAOSCC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9404_length_5470_cov_4.812004"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLG72_08765"
     CDS             <1..522
                     /locus_tag="PLG72_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ80925.1"
                     /translation="GGIVVSTSIKVAGDKFDEAIVRYMRKKHNIMVGERTAEEMKINI
                     GTVYPRVQEVSMDVRGRNLISGLPKTITVTSSEMMEALEEPMQAIIEAVLSVLERTPP
                     ELAADISDRGIVLTGGGSLVYGLDKLLQEKTGINVVIADDAISAVALGTGEALNNIEH
                     IEKSAAMEVRQKK"
     gene            765..1544
                     /locus_tag="PLG72_08770"
     CDS             765..1544
                     /locus_tag="PLG72_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOJ80926.1"
                     /translation="MVRGLYTSGWSMLALQKRMDVITNNMANADTYGFKKDTTVMESF
                     PDLLTKRLKYQASSSGRPRNIGTMELGSDIGEVYTYFSQGQLSKTDNELDIAIKDSDS
                     AFFTVAVPDADGGLAAFFTRDGSFVRNSDNVLVTSDGYYVLGENGPIMLQSGSFTVDE
                     KGYVIQDGEIIDRLLITEFTDTTALRKYGNNLIQADEDAQTRGFTGIIQQGYVELSNV
                     NIVREMVDMITVTRAYEANARVLQAIDSTLDKAVNQIGTVR"
     gene            1815..2615
                     /gene="flgG"
                     /locus_tag="PLG72_08775"
     CDS             1815..2615
                     /gene="flgG"
                     /locus_tag="PLG72_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOJ80927.1"
                     /translation="MMRALWTAASGMNAQQMTVDVIANNLANVNTTAYKKQRIEFKDL
                     LYETMDRARVLPEGGKPVNLQVGHGAMAAATVRDFEKGSPEETGNPLDFYIDGDAFFM
                     VEGPSGEILYTRDGSFKISMTDSGKMLATSDGYPVLDEAGMPIILDFDLSALTVSQNG
                     EISYLDEDGLTVPTGQYFGLVYFENRNGLEAVGRNLYKATPAAGDAIPAAESTETSIL
                     MQNFLEASNVKTVEEMVKLIVAQRAYELNSKAIQSSDEMLQTANNLRR"
     gene            2682..3095
                     /locus_tag="PLG72_08780"
     CDS             2682..3095
                     /locus_tag="PLG72_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOJ80928.1"
                     /translation="MDINMIDGMTIGNFVRNATNSATRASDDDFARRLEEAARNNDDR
                     ELKKACQEFEAIMLDMLYKQMKATIIKSDLVEEDPGRDIFESMLEEELMEQAAKRGSL
                     GLADTLYKQLSRQYGTSASANTSKNTKNNGSSGEE"
     gene            3085..4278
                     /locus_tag="PLG72_08785"
     CDS             3085..4278
                     /locus_tag="PLG72_08785"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ80929.1"
                     /translation="MKNKKNANKSILAIIAVLSLILVVEGFFILLGSRGDKGPDTDDP
                     AGGMPGETAEEPPAVDPKDHVKYMHRTESINELKQEINILEIDPGVPGIKIKPALSYD
                     LIYGYELFSKIASRHNAYAAVNGGFFRDFGLPSGMVVIDGQLMSAATGDYPVLVLRDS
                     RAELKEIDSELYIVVNGSDGYDKEAAKMIIDNINFPGGGKQTIVYTPVYGRTNRADKR
                     NITATVVEGVVKEIAEYEKEARIPGDGMLISFFDVAAYRGKELPVKVGDRVELVHEPD
                     MTGNVQAYECGCWLVRDGVPVVGDYDPWVGVLTNRDPRTAVGIKEDGTVILLTVDGRQ
                     PGYSAGFTGRELADYLVRCGAVDAAMLDGGASTAMLLEGKLVNRPSYKGQEREVAGAI
                     LVLLE"
     gene            complement(4351..5148)
                     /locus_tag="PLG72_08790"
     CDS             complement(4351..5148)
                     /locus_tag="PLG72_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80930.1"
                     /translation="MVEFFITENNEIEIRRYDADTTLADLAAELEAFRQTVPDLRTGK
                     CKGCGECCSDNIPVLGLDLAILDLDPAALCTGPGAKGANPAAASPNPEAQGTEPAVPD
                     FHPRALGGRHGPDTGQAAGSILVLPDKPDMAFRRKAIKEMCEWTSISELEATLLFEYN
                     NAEPLILARQASGECCFLKDGLCTRYTARPYSCGLYLCNMGRRLAYIQEMIVRQGTWH
                     AYSKLGWISEADIPHNPFLRAYSYDKLCVADFEFDLKSTSDQLFFYF"
BASE COUNT         1479 a         1270 c         1541 g         1180 t
ORIGIN      
        1 ggcggcatcg tagtgtcgac atccatcaag gtagccgggg acaagttcga cgaagcaata
       61 gtcaggtaca tgaggaagaa gcacaacatc atggtgggag aacgtacggc cgaagaaatg
      121 aaaatcaaca ttggtaccgt atatcccagg gtccaggaag tgtcgatgga tgtcaggggc
      181 cgaaacctta tttcagggct tcccaagaca ataacagtga cgtcgtctga aatgatggaa
      241 gccttggaag aacccatgca ggcgattata gaagctgtcc tttcggtcct tgagaggacg
      301 ccgccggaac tcgccgccga cataagcgac aggggcatag tgctgacggg cgggggcagc
      361 cttgtttatg gtttggacaa actgctgcag gaaaagaccg ggataaacgt ggtcatcgcc
      421 gatgacgcga tttccgcagt ggcgcttggt acaggcgaag cattgaacaa tatcgaacat
      481 atagaaaaga gcgctgcaat ggaagtaaga caaaagaagt aaaaggacac acgacatata
      541 agacagacat catgcaaaat gtaaaatgaa gccggtgaga tgccggcttt tctcattccc
      601 ctgcacaacc tcatgaaatc tccgcgtatc ttgtacgaag tgatttgctg tcatttttcg
      661 gttaagcctg gcggtacttt ttccgataat agtaatgaac caaccaatat tcaaagtatg
      721 ggacaggcct gcagcaatgc acaggcgggc gttcaggaga atttatggta cgtggattgt
      781 atacgtcagg ctggagcatg ctggcgctcc agaaaagaat ggatgtcata acgaacaata
      841 tggcgaatgc cgacacctat ggctttaaaa aggatacaac ggtcatggag agttttcctg
      901 atcttttgac caaaagactg aagtatcagg cttcttcttc cggaaggcca cgcaatatcg
      961 gcactatgga acttggaagc gacataggcg aggtatacac ctacttttcc cagggccagc
     1021 tgtccaaaac ggacaacgaa ctcgacatag ccatcaaaga cagcgacagc gcatttttca
     1081 ctgttgcggt gcctgatgcc gacggcggcc tggcagcgtt tttcaccagg gacggttctt
     1141 ttgtcagaaa cagcgacaac gtccttgtca caagcgacgg atattatgtg ctgggagaaa
     1201 atggtcccat catgcttcag agcggttcgt ttaccgtaga tgaaaaaggc tatgtcatac
     1261 aggacggaga gataatcgac agactgctta ttaccgagtt tacagacacg accgcgctga
     1321 ggaaatacgg gaacaacctg atacaggcgg atgaggatgc ccagactcgc ggatttacag
     1381 ggatcataca gcagggctat gtagagctgt caaatgtcaa tatcgttcgt gaaatggtag
     1441 acatgataac cgtgacaagg gcgtatgagg caaatgcaag ggtgcttcag gccatcgaca
     1501 gcacccttga caaggcggtc aaccagatcg ggacagtaag gtagaaaggt gcggtgacac
     1561 tcctctgcga ggaggtattc ctctgtggat gaggaatgtc acacgtttga aaagggtgcg
     1621 gtgacactcc tatgcaaaga tgtattcctc tgtggatgag gaatgtcaca cgtttgaaag
     1681 gggtgcggtg acactcctat gcgaagaggc attcctctat atataaggaa tgtcatcaaa
     1741 gctggtacta cataaggtcg ggaaatggag cacgttgtat agtaatacat tgtgattcag
     1801 gaggttcatc agttatgatg agagcactat ggacagcggc atcgggcatg aatgcgcagc
     1861 agatgacggt cgatgtgata gccaacaatc ttgccaatgt caatacaacc gcatataaaa
     1921 agcagcggat cgaattcaag gacctgctct atgaaaccat ggacagggcg cgtgtactgc
     1981 cggaaggcgg caagccggtg aacctgcagg tcgggcacgg cgccatggcg gccgcaactg
     2041 tcagggattt tgaaaaaggc agccctgaag aaacgggaaa tccgctggac ttctatatcg
     2101 acggggacgc atttttcatg gtcgagggcc cttcaggcga gattctctat acgagggacg
     2161 gcagtttcaa aataagtatg accgattcag gcaaaatgct cgccacttct gacgggtatc
     2221 cggtgctcga tgaagcaggt atgcccataa tacttgattt cgacctgtct gccctgactg
     2281 tatcacagaa cggtgagatc agttatctgg atgaggatgg tctgactgtc cccacaggac
     2341 agtacttcgg actggtttat tttgaaaaca ggaacggcct tgaagccgtg ggaaggaacc
     2401 tttacaaggc gacacccgca gccggcgatg caattccggc cgctgaatcg accgagacga
     2461 gcatcctgat gcagaatttc cttgaggctt caaatgtaaa gaccgtcgaa gagatggtca
     2521 agctcatcgt ggcacagagg gcatatgaac ttaactccaa ggcaatacag tcctcggatg
     2581 agatgctgca gaccgcgaac aacctcagga ggtaaggccc gttactgatc tgttgacatg
     2641 gcagccgcaa taaatatatt atttatgtca gagggattca aatggatata aacatgatcg
     2701 acggcatgac catcgggaat ttcgtcagga acgcgacgaa ctcggcaacc cgcgcgtccg
     2761 atgacgattt cgccaggaga cttgaggaag ctgccaggaa caacgacgac agggagctta
     2821 aaaaagcctg ccaggaattc gaggcaataa tgctcgacat gctctacaag caaatgaagg
     2881 cgaccataat caaatcagac cttgtggaag aggatccggg cagggatata ttcgaatcga
     2941 tgctcgaaga ggaactgatg gaacaggctg cgaaaagggg aagcctcggg ctggcagata
     3001 cgctgtacaa acagttgtcc aggcaatacg gaacaagcgc ttccgcaaac acatccaaaa
     3061 acacgaaaaa caacgggtcg tccggtgaag aataagaaaa atgcaaacaa aagcatactg
     3121 gcgataattg cagtattatc cctcatatta gtcgttgagg gattttttat tttgcttgga
     3181 tccaggggcg ataaggggcc ggatacggat gatcctgccg gaggaatgcc cggggagacg
     3241 gccgaagagc cgccggccgt cgatccgaag gatcatgtga aatatatgca ccggaccgaa
     3301 tcaataaatg agcttaaaca ggaaatcaac atccttgaaa tagatcccgg ggttcccggc
     3361 atcaaaataa agccggcact ttcctacgac ctgatatacg ggtatgaact gttcagcaaa
     3421 atagcttcca ggcacaatgc ctatgcggcc gtcaatggcg gctttttcag ggatttcggg
     3481 ctgccctcgg ggatggtggt tatcgacgga cagcttatgt cggcagcaac gggagattat
     3541 cctgtcctgg tactgcgcga cagcagggct gaattgaagg agatcgacag cgagctgtac
     3601 atcgtggtca atggatcaga cggatatgac aaggaagctg ccaaaatgat catcgacaac
     3661 atcaatttcc ccggcggcgg aaagcagacc atcgtttaca ctcccgtcta cggcaggacc
     3721 aacagggctg ataaaaggaa catcaccgca actgttgtgg aaggcgtcgt taaagagata
     3781 gcggaatatg agaaggaagc gcggataccc ggcgacggca tgctgatctc atttttcgat
     3841 gttgccgcat acagggggaa ggaattgccc gtaaaggtgg gcgacagggt ggaactcgtg
     3901 catgagcccg atatgacggg caatgtgcag gcatatgaat gcggatgctg gctcgtcagg
     3961 gacggagtgc cggtagtcgg ggattacgac ccgtgggtcg gggtactgac aaaccgtgac
     4021 ccgagaacgg ctgtcggtat caaagaagac ggaacggtca tactgcttac cgtcgacggc
     4081 aggcagccgg gttacagcgc aggatttacc ggccgtgagc ttgccgatta cctggtacgc
     4141 tgcggcgccg tggatgcggc gatgctggac ggcggggctt ccaccgcgat gctgctggaa
     4201 gggaagctcg tcaacaggcc ttcttataaa gggcaggaaa gggaagtagc cggcgccatc
     4261 ctcgtattgc tggaataggc ctgtaccggc gataaaagcc ttgggccccg aattgttcaa
     4321 aatgattaca attgcgtaaa ataaagctgc ttaaaaatag aagaacaact ggtccgacgt
     4381 gcttttcaga tcgaattcga aatcggcgac acacagttta tcatatgaat acgccctgag
     4441 gaacgggttg tgcgggatgt cggcctcgct gatccagccg agtttgctgt atgcgtgcca
     4501 ggttccctgc ctgactatca tttcctggat gtatgcaaga cgcctgccca tgttgcacag
     4561 gtagaggccg cagctgtacg gacgtgccgt ataccttgta cagaggccgt ctttgaggaa
     4621 acagcattcg cctgatgcct gcctggcaag tatcagcggc tccgcattgt tgtattcgaa
     4681 cagcagcgtt gcttccagtt ccgaaatgga ggtccattcg cacatttctt ttatggcttt
     4741 tctgcggaag gccatgtccg gtttgtcagg cagcaccagt atacttccgg cggcctgccc
     4801 ggtatccggg ccgtgtctgc cgcccagtgc ccggggatga aagtcgggta ccgctggttc
     4861 tgtaccctgc gcttcgggat tagggctggc tgcggccgga ttggcgccct ttgctccggg
     4921 accggtacat aatgcagcag gatcgaggtc aagtattgca agatcgaggc caagcaccgg
     4981 tatgttgtcg ctgcagcatt cgccgcagcc tttgcatttc cctgtcctga gatccggcac
     5041 agtctgccgg aatgcctcca gttccgctgc caggtcggcc agcgtggtat cggcgtcata
     5101 tctcctgatt tctatttcgt tgttttcggt gatgaaaaat tcgaccattg ccgttcctcc
     5161 ttccttaaag tataccagat gcgccgttgc cgctcaacaa gtcccgggac gcccagcctg
     5221 gatacatggc tgtgtaagac cggtcaaaat tacagcatga aataaccgga ggaccggaac
     5281 ctttatccag aaccgggggt ctaatatcat gtactgccgg ttgcggcatg acacaaagcg
     5341 ggagtcccgt tgaatacccg ctgaagtgag tggacttttc agcaaccggc ggatttctgc
     5401 cgattgatga aaaaaatgaa agaccagcta attaaagtca agagttttta agaaaaagct
     5461 ttatgaattt
//
LOCUS       DAOSCC010000166         5461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9429_length_5461_cov_4.733444, whole genome shotgun sequence.
ACCESSION   DAOSCC010000166 DAOSCC010000000
VERSION     DAOSCC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9429_length_5461_cov_4.733444"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLG72_08795"
     CDS             <1..454
                     /locus_tag="PLG72_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ80931.1"
                     /translation="GIRESRRICGRTVITIQDALNGARYADCIGATTYNFDLPDPLKP
                     SYDPMMGDAKNPNAKRKHIVIRIPYRSLLPVEVDNLIVAGRCISAEREVLGAIRVMGP
                     CMQMGQAAGTAAASAVSAGCDFSRIDTDQLRETLWKDGVLNPDELPFE"
     gene            534..995
                     /locus_tag="PLG72_08800"
     CDS             534..995
                     /locus_tag="PLG72_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOJ80932.1"
                     /translation="MIVDSLKNAKLYIPLHPGFKKAFDFLEACEADFPPVGKHIIDGT
                     SVYASVQEYDTSPEENNIWEAHRKYIDIQFIRSGHELIGYTNAENIKDFSSYNCEKDC
                     MTAAKAGNAAFIDMLPGYFAIFYPNDAHKPKCIAGTKTHVQKIVVKVLLDQ"
     gene            complement(1164..1240)
                     /locus_tag="PLG72_08805"
     tRNA            complement(1164..1240)
                     /locus_tag="PLG72_08805"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1204..1206),aa:His,seq:gtg)
     gene            complement(1380..3311)
                     /locus_tag="PLG72_08810"
     CDS             complement(1380..3311)
                     /locus_tag="PLG72_08810"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ80933.1"
                     /translation="MKHGFVRVAAAVPELKVGACSYNTGKISEMIREAENAGVEIIVF
                     PELSITAYTCADLFQQDRLLRSALDGLEKIARLTGDMKIVVIAGLPLLLDNQLFDCAA
                     VIQGGEILGVIPKTYIPGYSEFYEERWFASARNAQSDHIELLGRKVPFGTDLLFVSQD
                     SGAACFGVEICEDLWIPVPPSSYLAAAGAAVIFNLSASNEVIGKTEYRRDLVRMQSGR
                     CIAGYVYASSGVHESTTDVVFGGHALIAENGGVLCESERFRRDSHMIMTELDIQRLMN
                     NRIKNTSYMDGVSRQSFRKIPFTLYRNDDLKLTRHVDPHPFIPADKQGRDERCREIFA
                     IQTAGLAKRLEHTGIEKAVIGISGGLDSTLAMMVTAKAFDMLRLPRKNILAITMPGFG
                     TTDITHDNAVRLINSLGAEFREINIVKACMQHFEDIGHDPSIHDVTYENVQARERTQI
                     LMDVANKINGLVIGTGDLSELALGWCTYNGDHMSMYAVNCGIPKTLVRHLVRYVAEST
                     DDTAVSDVLLKILDTPVTPELLPPDKNGEIRQKTEDIIGPYELHDFFIYHMLRYGASP
                     AKILYLAEHAFEGRYDTHTILTWLKVFIRRFFSQQFKRSCLPDGPKVGTISLSPRGDW
                     RMPSDVSAEEWLRELDALE"
     gene            complement(3499..>5461)
                     /locus_tag="PLG72_08815"
     CDS             complement(3499..>5461)
                     /locus_tag="PLG72_08815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ80934.1"
                     /translation="NPEDERYRDLVGKTVILPLMDREIPVIADSYVDKEFGTGVVKIT
                     PAHDPNDFEVGLRHGLQQIRVMDDRAVMNENAGRYAGMGRYEARKKIVEDLKELGLLA
                     KIEGHKHNVGTCYRCGTVIEPILSRQWFVKMKPLAEPAIEVVKDGTVKFVPERFSKIY
                     FNWMENIQDWCISRQLWWGHRIPAYYCQGCGEMIVAREQPDRCPKCGSTNLVQDPDTL
                     DTWFSSALWPFSTLGWPEKTRDLEYFYPTDVLVTGYDIIFFWVARMIFSALEHTGREP
                     FKYVFIHGIVRDAQGRKMSKSLGNGIDPLEVIEQYGTDALRFALTTGNSPGNDLRFSS
                     EKVEAGRNFANKIWNATRFVLMNFDENVDFGRVDRNKLTTADKWILSRTNTVVKEVTE
                     NLENFELGIALQKIYEFIWEEFCDWYIELVKPRLFDRDDPTRLEAQWVLNHVLGTAMK
                     LLHPYMPFVTEEIYRHLINDDDSIMISAWPVYDSSMEFAEDEKDMELVMDAIKAVRNI
                     KAEMNVPPAKKIKLIFVAGESESRILKDGTKFIERLAGASEILIQADKAGVPDDAVTA
                     VVPGAEIYIPLEDLVDIAKELERLAKEKENLEKELERVNGKLSNEGFVSKAPAKVVEE
                     ERAKKAKYSEMYEKVLARIASLEKLGGKA"
BASE COUNT         1186 a         1555 c         1307 g         1413 t
ORIGIN      
        1 cggtatccgt gagtcacgcc gcatttgcgg aagaaccgta attacaatac aggacgcgct
       61 caatggagcc aggtatgcgg actgcatagg tgcaaccact tataatttcg atttgcctga
      121 tcctttgaaa ccaagttacg atcccatgat gggggatgca aaaaacccca atgcgaagcg
      181 caaacatatc gttatccgga taccataccg aagcctgctg cctgtggaag tcgacaacct
      241 gatcgtcgca ggcaggtgca tcagtgcaga acgtgaggtt ctcggagcaa tccgggttat
      301 ggggccatgt atgcaaatgg gacaggctgc cggtacggca gctgcctcag ctgtttctgc
      361 aggctgcgat ttcagcagga tagataccga tcaacttcgg gagactttat ggaaggatgg
      421 cgtacttaat cctgacgaac tgccgtttga atagcctcat atatcgttgc tgctcagacg
      481 gcctttaacc gggtatcggg tcgtctgagc acagcactgc aaggaggacg tttatgatcg
      541 tagattcatt gaaaaatgca aaactgtata tacccctgca ccctggcttc aaaaaggctt
      601 ttgactttct ggaagcctgc gaggcagact ttcctcctgt cggaaagcat atcatagatg
      661 ggacaagcgt ctatgcttcc gtacaggaat acgatacctc tcctgaagaa aacaacatct
      721 gggaggcgca tcgcaaatat atcgatatcc agtttataag atccggtcat gaactgatcg
      781 gttacaccaa cgcagagaat atcaaggact tcagttcata caactgcgaa aaggattgca
      841 tgaccgcagc caaagccggc aatgcggcat ttatcgatat gcttcccgga tattttgcaa
      901 tattttatcc taatgacgct cataaaccga aatgtatagc gggcacgaag acccatgtcc
      961 agaaaatcgt ggtgaaggtg ctgcttgatc agtgaccggg acttcgcatc ccttctatgg
     1021 accagcccga tatggacatc catgtatttg attaaatctc tttgccgcca gattccatag
     1081 cggactttca ccgactggtg cggtccagct ttgctggatg cgccgcatat aaaaaaagag
     1141 gttgtatcaa caacctctcc tgttggggtg agctaaggga attgaaccct tgacctccag
     1201 ggccacaacc tggcactctg accaactgag ctaagctcac cgcatatatg cgctttgaca
     1261 gcgacatatg ctattgtaaa gcacgaagcc ggttttgtca agtaacaatt aacccggccg
     1321 tttcacctgg aagcagccga tattctcttg actccagtat cattccatgg ctccaggtat
     1381 cactcaaggg catcaagttc cctgagccac tcctcggccg aaacgtcgct gggcatgcgc
     1441 cagtctcccc tcggcgaaag gctgatcgta ccgacctttg gcccgtcagg caggcaggag
     1501 cgcttgaact gctgcgagaa gaacctcctg ataaatacct tcagccatgt aagtatggta
     1561 tgcgtatcat acctgccctc aaaggcatgt tccgcaaggt acagtatctt tgcgggcgaa
     1621 gcgccgtacc gcagcatgtg atagatgaaa aagtcgtgca gttcgtaagg gccgatgata
     1681 tcttctgtct tttgccttat ctccccgttt ttgtccgggg gcagaagttc aggcgtcacc
     1741 ggtgtgtcaa ggatcttcag cagcacgtcc gaaacagccg tatcgtcagt gctttcggca
     1801 acataccgca caagatgcct gacaagggtc ttcgggatcc cgcagtttac ggcatacatg
     1861 gacatgtgat ctccgttgta tgtgcaccat ccgagagcga gctcggaaag gtcacctgtg
     1921 cctattacaa gcccgttgat cttgttggcc acgtccatga gtatctgtgt gcgctccctc
     1981 gcctggacat tctcgtaagt gacatcgtga atcgaggggt cgtggccgat atcttcaaag
     2041 tgctgcatgc atgcctttac gatgttgatc tcgcggaact cggccccaag cgagttgatg
     2101 agccgtacgg cattgtcatg cgtgatatcg gtggtgccga acccgggcat cgttatcgcc
     2161 agtatattct ttcttggcag ccgcagcata tcgaatgcct ttgcggtgac catcatcgcc
     2221 agcgtggaat caagcccgcc cgatatgcct ataactgcct tttcgatgcc ggtatgttca
     2281 agcctttttg caaggcctgc agtctgtatc gcaaatatct ccctgcacct ttcatccctt
     2341 ccctgtttgt ccgcagggat aaacgggtgc ggatccacat gccttgtaag cttcaagtca
     2401 tcattcctgt aaagcgtgaa gggtatcttc ctgaaacttt gccttgaaac gccgtccata
     2461 taactggtgt tctttatcct gttgttcata agtcgctgta tgtcgagttc ggtcattatc
     2521 atgtggctgt cgcggcggaa cctttcggat tcgcacagca caccgccgtt ttcggcgatc
     2581 agtgcatggc cgccgaatac tacatcggtg gtggactcat ggacaccgct cgatgcataa
     2641 acatatcctg cgatgcatct gcccgactgc atcctgacca ggtccctcct gtattccgtc
     2701 ttaccgatca cctcgttgct ggcggaaaga ttgaatatga cggcagcgcc tgccgcagcc
     2761 aggtaagaac ttggagggac ggggatccac agatcttcgc atatttcgac tccaaagcag
     2821 gcagcgccgg agtcctgcga cacgaacagc agatccgttc caaacggtac cttcctgccg
     2881 agcaattcga tatggtcgct ctgggcattc cttgcagacg cgaaccagcg ttcctcgtaa
     2941 aactcgctgt agcccggtat atatgtttta gggatcacgc cgagtatctc cccgccctgg
     3001 atcacagcgg cgcagtcgaa cagctggttg tccagcagca gcggcagtcc ggctatgacg
     3061 actattttca tgtcgccggt cagccgcgcg atcttctcaa gcccgtccag ggccgacctg
     3121 agcagcctgt cctgctggaa caggtccgca catgtatagg cagtgatcga cagttccggg
     3181 aacacgatta tttcgacgcc cgcgttctcg gcctccctga tcatctctga aatttttccg
     3241 gtgttatagc tgcaggctcc cactttcagt tccggtaccg ccgcagcgac cctgacaaat
     3301 ccgtgtttca tactatccgc tcctgcttcc ataccgagtt tccatacctt atgctgacta
     3361 tattataaca cttatgaaca ttataggata catgcggcct gttcgcatga cgaacgccat
     3421 atcgcccaac ggtacgggct ttgtcatact gaaaacgcct gcagcatcat ccgcaggcgt
     3481 tcagtgctga aatccggatc aggcctttcc tcccagcttt tcaaggcttg cgatgcgcgc
     3541 aaggaccttt tcgtacatct cgctgtattt tgccttcttg gcccgttcct cttcgaccac
     3601 cttcgctgga gccttggaaa cgaacccttc gttgcttagc ttgccgttta ccctctccag
     3661 ttccttctcg aggttttcct tttctttcgc tagcctctcg agctcttttg cgatatccac
     3721 aaggtcttcc agcgggatgt atatttccgc gccgggcacc actgctgtca cggcatcgtc
     3781 aggcacaccg gccttgtcgg cctggataag gatctccgaa gctccggcaa ggcgttcgat
     3841 gaatttcgtg ccatccttca gtatcctgct ctcggattct ccagcgacga atatcagctt
     3901 tatcttcttt gccggcggca cattcatctc tgcctttatg ttcctgaccg cctttattgc
     3961 gtccatgacg agctccatat ccttctcatc ttccgcaaat tccatgctgc tgtcgtatac
     4021 aggccaagcg gagatcatga tgctgtcgtc gtcattgatg agatgcctgt atatttcctc
     4081 ggtgacgaac ggcatatacg ggtgcagcag cttcatggca gtgccgagga catggttcag
     4141 cacccactgc gcttcgagcc tcgtgggatc gtccctgtcg aagagccttg gcttcacgag
     4201 ctcgatatac cagtcgcaga actcctccca gatgaattcg tatattttct ggagcgcgat
     4261 gccaagttcg aaattctcaa ggttctcggt aacttccttc acgacggtat tggtcctgct
     4321 gagtatccac ttgtcggccg tcgttaattt gttcctgtct accctgccga agtccacgtt
     4381 ttcatcgaag ttcatcagca cgaatctcgt agcgttccat atcttgttgg caaaattcct
     4441 gcctgcctcg accttctccg atgagaacct caggtcgttt ccaggagaat tgcccgtagt
     4501 cagggcgaac ctcagcgcat ccgtaccgta ctgttcgatg acttccagcg ggtctatgcc
     4561 gttcccgagg gatttgctca tcttcctgcc ctgcgcgtcc cttacaatgc cgtggatgaa
     4621 cacgtatttg aacggctctc tgcctgtatg ctcaagggcc gagaatatca tcctcgctac
     4681 ccagaagaat atgatgtcat acccggtcac aagtacatcc gtcggataga aatactcgag
     4741 atccctcgtc ttctccggcc atcccagcgt cgagaacggc cacagcgccg aactgaacca
     4801 tgtatccagc gtgtcaggat cctgtaccag gttcgtgctt ccgcatttcg gacatctgtc
     4861 aggctgctcg cgcgcaacaa tcatctcccc gcatccctgg cagtaatacg caggtatgcg
     4921 gtggccccac caaagctgcc tcgatatgca ccagtcctgg atgttttcca tccagttgaa
     4981 atatatcttc gaaaatctct cgggaacgaa tttgaccgtc ccgtccttca ccacttctat
     5041 cgccggctct gccaggggtt tcatctttac gaaccactgc cttgacagga tcggctcgat
     5101 aacggtgccg cacctgtaac atgtgcctac attatgctta tggccttcga tcttcgcaag
     5161 caggccgagt tctttcagat cctcgacaat tttctttctt gcctcgtacc ttcccatacc
     5221 tgcataccgg cctgcatttt cgttcatcac ggccctgtcg tccatgaccc ttatctgctg
     5281 cagcccatgt cgcaggccta cttcaaagtc gttgggatcg tgtgccggcg ttatcttgac
     5341 gacgcctgtc ccgaactctt tgtcaacgta ggagtccgca atgaccggta tctccctgtc
     5401 cataagcggc agtatgaccg tcttgcctac caggtccctg tacctctcat cttccgggtt
     5461 g
//
LOCUS       DAOSCC010000167         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9484_length_5439_cov_4.755944, whole genome shotgun sequence.
ACCESSION   DAOSCC010000167 DAOSCC010000000
VERSION     DAOSCC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9484_length_5439_cov_4.755944"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLG72_08820"
     CDS             complement(<1..899)
                     /locus_tag="PLG72_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOJ80935.1"
                     /translation="MRKNLTALLLVILMLVSVAGCSTTKAVSALPQSVKNISGLTANN
                     TESQPIAKNPAESLKNSTEKLPEDPSFEIRFLDVGQADAALIKCDGKAMLIDGGNSED
                     SNLIYSVLKAQKITHLDYIIATHAHEDHVGGLAGALNAAAAGTVYSPVDSYDSKAFKN
                     FLKYVRKQGLELTYPPVGKEISLGSATVQFIGPQKDYEDPNNTSIVVRIVYGETSFLF
                     TGDAETEAEHDIIDAGYDLSSTVLKVGHHGSEASTSYVFLREVMPEYAVISVGKDNPY
                     GHPHDNTLSRLRDAGVIVYRTDM"
     gene            1066..1254
                     /locus_tag="PLG72_08825"
                     /pseudo
     CDS             1066..1254
                     /locus_tag="PLG72_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1391..1633)
                     /locus_tag="PLG72_08830"
     CDS             complement(1391..1633)
                     /locus_tag="PLG72_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80936.1"
                     /translation="MAMAALISIGKFFAKLILFVIFSPVIFTWMLFRCLRCRTVLVRN
                     MVQAGMPREYALELAREIRISRMLGFSMDGKGHKPD"
     gene            complement(1637..1933)
                     /locus_tag="PLG72_08835"
     CDS             complement(1637..1933)
                     /locus_tag="PLG72_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80937.1"
                     /translation="MSSDIPVKEIGELLDAVSDKAPKIITSLMDTLYSAESAKKMGQA
                     VGSFYRELVESGIPQEEALKLARDYMLSLKDMTGSFQNNAPDYNAAYKQADCSK"
     gene            complement(1946..2281)
                     /locus_tag="PLG72_08840"
     CDS             complement(1946..2281)
                     /locus_tag="PLG72_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ80938.1"
                     /translation="MDECERVSELLLDYMNRRLSGNENIAAAKHLAECRRCRNELAVL
                     LRIRNLAHEGISPIPQDIADTAFDKIPDTKGTLAAALNLRQYFKAFDYLHDAMSVVNH
                     VMKLAQQAM"
     gene            complement(2268..2855)
                     /locus_tag="PLG72_08845"
     CDS             complement(2268..2855)
                     /locus_tag="PLG72_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ80939.1"
                     /translation="MKAVKRGGADGFSPESCTESTLIRKAGNGGKEAFEVLMKGLLDI
                     IYSYISSHVGTAEDVKDILQETMLSVWNGLKSYDNRSSFKTWVIGITRRKIADHYRMR
                     YRDAADRLSEFEDILSSDDDFERINSRNDINAAMAVLDKTEREIVFLAFNVQLTYPEI
                     SEILGIPTGTVKSRMSSIKAKLRKQLDKEGGYGRV"
     gene            3174..3539
                     /locus_tag="PLG72_08850"
     CDS             3174..3539
                     /locus_tag="PLG72_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ80940.1"
                     /translation="MVEYRLGAMETRFAELIWDNEPISSGELVRLCEKELAWKKSTTY
                     TMLRRLCQRGIFQNVNGTVSALMSRQEFYALQSEKFVEETFEGSLPKFLAAFTLRKKL
                     TEKEIKELQKLIDEKRGEY"
     gene            3539..5170
                     /locus_tag="PLG72_08855"
     CDS             3539..5170
                     /locus_tag="PLG72_08855"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ80941.1"
                     /translation="MRMYALLVSILNMSLTGGIAIVFVMLARILLRKAPKIFSYALWA
                     VVLFRLVCPVSFSSAFSVMGLLRMPAEENGGIPFISTDIVHTSDIVRNEYLRADMPSP
                     DSDEVKIGSLLQDAQLLPADPPEAPVAVATMLWIFGMAVMLAYSVVSLLMLRQRLIGS
                     VCLRDNIFLADHIPTPFVMGVLKPRIYIPSSLPENEQGYVILHEKIHIRRFDHIVKIL
                     AFIVLAIHWFNPLVWTAFVLCMKDMEMSCDERVLKEMGEGIRFDYSNSMLSLAAGRRL
                     ISGSPLAFGEWNVKERIRNVLNFKKPAVWIIAVSLVFVAALGFALAANRAGDKAEGDL
                     HEISGNQEDISGIPDEKEIRLASKQEQMMMSRNNMEIVVPISPELSAGLQIGADMTEL
                     DYASDEIVIFHDYFGLFVYDIELKKIVRSLDLESIGCHFTQGDNYCEVAVSEDGKTVQ
                     LHPMSSDRMYVYSVPDKLLVKKPYKSMDGRFDDFVDITNIVDYSEAGKCSHTAVSFDN
                     GDYGYLYTSDWTLDTLYYVRGDSTYKLFDFEETSD"
BASE COUNT         1336 a         1259 c         1364 g         1480 t
ORIGIN      
        1 tgcatatcgg tgcggtaaac gattacaccg gcatcgcgca agcggctaag ggtattatcg
       61 tgaggatgtc cataagggtt gtctttccca acagaaatta cggcatattc tggcatgact
      121 tctctgagga aaacatatga ggttgatgcc tcgctgccgt ggtggccaac cttcagaaca
      181 gtagagctta agtcatatcc tgcgtcaata atgtcatgtt cggcttccgt ttctgcatca
      241 cccgtaaaca gaaatgaagt ttcaccatat actatcctga ctactatcga tgtgttattc
      301 gggtcctcat aatccttttg cgggccgata aactgcaccg tcgcactgcc gagagatatt
      361 tcttttccaa caggcggata cgtaagctca agaccttgct ttcttacata tttcaaaaag
      421 ttcttaaagg ctttactgtc atatgaatct acaggactgt agaccgtacc ggctgcagcg
      481 gcgttaagcg caccagccag accgccgaca tggtcttcat gcgcatgcgt agctatgata
      541 taatcaaggt gtgttatttt ctgagctttc aatactgaat agataaggtt tgaatcctca
      601 gagttcccac catcgatcag cattgcttta ccgtcgcatt ttatcaaagc tgcgtcggcc
      661 tggccgacat ccaggaaccg tatctcaaat gacggatctt ccggcagctt ttctgttgaa
      721 ttttttaatg attctgccgg attttttgca attggctgag attcagtatt attagcggtt
      781 aacccggaaa tgttcttaac ggactggggt aaagcagata ctgctttggt tgtactgcac
      841 cctgcgacag aaaccagcat caaaataacc aataacagag ctgtcaaatt ttttctcatt
      901 agtccagcca ccttccggat aagtaaaaaa tatatattta taatattcat cggcatttga
      961 tttttttcca taagaatggc ttgataaagg gaaagatcgt ttatattcaa gagatgtgca
     1021 tatctataag ttactgaata attgagcgga ccgcctgatt caaagcaggg agagccttat
     1081 gaagatgaga agttcggcga agcgatcgaa gagttgtggg aagtaatgaa aaagtatgac
     1141 ccgcggatct acggatttga atgggcggat gaggatgcgc cgagattcca gcttgaaccg
     1201 cagggttaca ggggttatat cgaagccagg ccggttaggc cgatacaaag gtaagtgcgg
     1261 cgtaaacaga cagaggaaat ttcttagaca aacaaggaaa ggtttccgga cagacagggg
     1321 ggacggttcc cgtgcccggt caatgcagga cccggacaca ggagccgtcc ctccggctcg
     1381 ttattttcac ttaatcaggt ttatggccct ttccatccat gctgaatcca agcatgcggc
     1441 tgatacggat ctccctggca agttccagtg cgtattccct tggcatgccc gcctgtacca
     1501 tgttgcgtac aagtacagtc ctgcagcgaa ggcagcggaa cagcatccag gtgaagatca
     1561 ccggggaaaa aatgacgaac agtatgagtt ttgcaaagaa cttgccaatg cttatcaaag
     1621 ccgccattgc cataccctat ttgctgcaat ctgcctgttt gtacgccgcg ttataatcag
     1681 gggcattgtt ttggaatgag cctgtcatat ctttcagcga cagcatgtaa tccctggcca
     1741 gctttagtgc ttcctcctgc gggatgcctg actctaccag ttccctgtag aagctgccga
     1801 ccgcctgccc catctttttc gccgattcgg ctgaatagag cgtatccatg aggcttgtta
     1861 tgatttttgg cgctttatcg ctgactgcat ccagcaattc gccgatttct tttaccggga
     1921 tatcacttga cataaaatct cctccttaca ttgcctgctg tgcaagcttc attacatggt
     1981 ttacaaccga catggcatca tgcagataat cgaatgcttt gaaatattgc cttaagttga
     2041 gtgctgcagc aagcgtacct ttagtatcag gtattttgtc gaaagcagta tcggcgatat
     2101 cctgcgggat aggtgatatg ccttcatgtg caaggttcct gatcctgagg agtactgcaa
     2161 gctcattcct gcagcgccgg cactctgcaa gatgttttgc tgcagcgata ttctcattcc
     2221 cggacaatct acggttcatg taatccagca gcagttcaga gactctttca cactcgtcca
     2281 tatccaccct ccttgtcaag ctgtttcctc agtttcgctt ttattgacga catcctgctt
     2341 ttgacggtgc cggtgggaat tcccaatatt tcagaaattt ccggatatgt aagctgtacg
     2401 ttgaaagcca ggaacacgat ttccctctcg gttttgtcga gcacggccat ggccgcgttt
     2461 atgtcattcc tgctgttgat gcgctcaaaa tcatcatctg aagataaaat atcttcaaat
     2521 tccgataacc ggtccgctgc gtccctgtaa cgcattcggt aatgatcggc gatcttgcgc
     2581 ctggttatcc cgatcaccca ggttttgaag gacgacctgt tgtcataact tttcagcccg
     2641 ttccagaccg aaagcatcgt ttcctgcagg atatccttga cgtcttctgc tgtgccgacg
     2701 tgagaggaaa tatagctgta aatgatgtcc agcaagccct tcatcagcac ttcgaatgct
     2761 tctttgccgc cgtttccggc tttcctgatc agggtgcttt ccgtacagct ttcaggggaa
     2821 aaaccatcag caccacctcg ctttactgct ttcacttact tagtcggaga aaaagggcaa
     2881 acggttcagg ttattttatg cagtacagag aaaatactgc ttgacatttt ggtttaatga
     2941 cgttaaactg ataatacggt ttaatcatat tagaccaaaa catccggccg tgcttcgggc
     3001 tgattgccgg caggcttgaa gtgcggttgg gcgccgctgt caccttggtg agacggcgca
     3061 ctgccttagc cgggcggcgt attcgcttca gccggatggc gtgtttgcct cagccgtgcg
     3121 gcgcatttgc ccgggtcagg cggcaatcag agtgatcggg agaggtgtcg gtaatggtag
     3181 agtacaggct tggcgccatg gaaacaaggt ttgccgagtt gatatgggat aatgagccta
     3241 tatcttccgg tgaattggta aggctttgcg aaaaagaact tgcgtggaaa aaatcgacga
     3301 cttatacgat gctgcggagg ctttgccagc gcggcatctt tcagaacgta aacggaaccg
     3361 tatccgccct tatgagcagg caggagttct atgcgctgca aagcgagaag tttgtcgagg
     3421 agaccttcga aggctcgctt cccaaattcc tggctgcgtt caccctcaga aaaaagctga
     3481 ctgagaaaga gattaaagaa ctgcagaagc tcattgatga aaagaggggt gaatactgat
     3541 gcggatgtac gcgttgctgg tcagtatcct caatatgagc ctgacaggcg gaatagccat
     3601 tgtctttgta atgctggcac gaatactgct caggaaagcg ccgaaaatat tctcttatgc
     3661 actctgggca gtcgtcctgt ttaggcttgt ctgcccggta tcattttcgt cagctttttc
     3721 cgtgatgggt ctgctgcgta tgccggccga ggaaaacgga ggcattccgt tcatttctac
     3781 cgatatcgtg catacaagcg atatcgtacg taacgaatac cttcgtgcgg atatgccatc
     3841 tccggattct gatgaagtca aaatcggcag cctgttgcag gacgcccagc tattgcctgc
     3901 ggaccctcct gaagcccccg tggctgttgc cacgatgctc tggattttcg gcatggccgt
     3961 gatgctggca tacagtgttg tgtctctttt gatgctgcgc caaaggctaa taggctctgt
     4021 gtgcctgcgt gacaatatat tccttgctga ccacattcca actccatttg ttatgggggt
     4081 gctgaaaccg aggatctata ttccgtcatc actgcctgag aatgaacagg gctatgttat
     4141 acttcatgaa aagatacata tcaggcggtt cgaccatatt gtcaaaatcc ttgcctttat
     4201 cgtgcttgca atacactggt tcaatccgtt ggtttggacg gcgtttgtat tatgcatgaa
     4261 ggatatggag atgtcctgcg acgaacgtgt tcttaaggag atgggcgagg ggatcaggtt
     4321 cgattattcg aattcgatgc tgtcccttgc ggcggggcgt cggctcatca gcggcagccc
     4381 gcttgcgttt ggcgaatgga atgtaaagga acgtattaga aatgtgctga acttcaaaaa
     4441 gcctgccgta tggatcattg cagtttcgct tgtgtttgtg gctgccctgg gcttcgcact
     4501 ggcggccaac agggctgggg ataaagcgga aggagacttg cacgaaattt ccgggaacca
     4561 ggaagatata tcgggaatac cggatgaaaa agaaatcagg ctggcaagca aacaggagca
     4621 gatgatgatg tcaaggaata atatggaaat tgtcgtgcct atatcaccgg aactgtcggc
     4681 cggactgcag attggcgccg atatgactga acttgattac gcctcggatg agatcgtgat
     4741 tttccatgat tatttcggct tgttcgtata tgacattgaa ctgaaaaaaa tcgtccgcag
     4801 cctggatctg gaatcgatcg ggtgccattt cacgcaaggt gacaactact gtgaggtggc
     4861 tgtaagcgaa gacgggaaaa cggtccagct tcatcccatg agcagcgaca ggatgtatgt
     4921 atattcggtc ccggataaac ttctggtgaa aaagccgtat aagagcatgg atggccggtt
     4981 tgacgatttt gttgacataa cgaatatagt agactacagc gaagccggaa aatgcagcca
     5041 taccgctgtc agcttcgaca acggcgacta tggctacctt tatacttctg actggacgct
     5101 ggatactctt tattatgtcc ggggagattc gacgtataaa ttgttcgatt tcgaagagac
     5161 ctctgattga gttggtgtaa agagacgcct gactgagtcg gtttcagcct gaccaggtcc
     5221 ggcggatgac ttgtccgctc aaatccgggc agtctatctg cccgccggga tcgggacaga
     5281 taaatagaga ttcttgttgc tgtatccctt tgtctcctaa aatacaacca ctgattttat
     5341 atcgatgagt gtctttattt ccataggtct tttcctcagg ctgcaggccg tttcccccag
     5401 gttgtcgcca ggcttgacga ccgaatcggc ataatcgtt
//
LOCUS       DAOSCC010000168         5376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9643_length_5376_cov_4.444277, whole genome shotgun sequence.
ACCESSION   DAOSCC010000168 DAOSCC010000000
VERSION     DAOSCC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9643_length_5376_cov_4.444277"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1136
                     /locus_tag="PLG72_08860"
     CDS             213..1136
                     /locus_tag="PLG72_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ80942.1"
                     /translation="MAPKLDQYRIFCEVAKCRSFSRAAKRLYMTQPAVSQAVMSLESE
                     LGVRLFTRTSTGVTLTNDGQLLFEHVNSAINLIHQGENKLLESRDLKVGEMKVGVGDT
                     ISRYFLLPYLEIFHSRYPNIKLNIVNRTTTELCALVKSGEIDIAVCNFPVKDDALETK
                     ECIGIHDIFVCGAKYIGQFREPAGLDVIAGMPLILLERKSNSRLYVEKYLLSQGIRVT
                     PEIELGSHDLLLEFASFNFGIACVVEEFSREYLSSSRVFRIKLTREIPPRSIGFCHLK
                     SVPLSPAAQKLVEVVFSELKSGRTVKGYGQE"
     gene            1695..2726
                     /locus_tag="PLG72_08865"
     CDS             1695..2726
                     /locus_tag="PLG72_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOJ80943.1"
                     /translation="MAIITISRQDGSYGSEIAAKLSEKLGMRLILKDTVLNEWMREVT
                     NDYQLRMLTESPKFYNNVMDCGLTYKEYIERKLVSEAEKSPCVIVGMGSQIIFAGHPD
                     ALNIRIIASDGTRVERLRQKYGLGAREAERLMQQGDRRHRRYISVLYGADWDDVRHYD
                     MILRTDDLTVDMCVEIISGTAVMKDKLKASGQAVPGTEPAPAVRRGEIRFKHPAEEEF
                     AKILDMYGIEWQYEPRTFPVGWDAEGNVKMAIKPDFYLVKFDTYVEITTMDQKYVTTK
                     NKKVRMLRELYPDININIVYKKDFHSLLKRFGFGGEDEIDEFNRNEKNTVQRRDDKEQ
                     GEGTGQADQ"
     gene            2641..3201
                     /gene="hpt"
                     /locus_tag="PLG72_08870"
     CDS             2641..3201
                     /gene="hpt"
                     /locus_tag="PLG72_08870"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ80944.1"
                     /translation="MNLTGMKRILFSEEMIKNRVKELGRQISEDYRDKHPILISVLKA
                     TMYFVADLTRELDIPVNIDFMSIGVYPGITKNTGVVRINKDLDLNITGRHVLMVEDII
                     NTGLTIGYLVQNMEARKPASIKICTLLNNPSKRLVNIPLEYVGFEVPDVLLVGYGIDY
                     SQEYRHLPYIAEFSEEEYLKANSGGV"
     gene            3386..4174
                     /locus_tag="PLG72_08875"
     CDS             3386..4174
                     /locus_tag="PLG72_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80945.1"
                     /translation="MSSKAVVSLIAALAISVSGIVRPVAALAAEEGANAAVTGSGNSL
                     TESDSVLSEEVKASGYYAALAAVSLLKQAGMILGDPVVAAAAADVKKAADTAASLSGL
                     PIQAGASAEDDESLWYCPGIPMKKEHQKLLWECCRERGLDYIDMLALIALESNFQEKC
                     SNGKYKGYFQISSDHGTTLSRQLNTKNDPLDGTVNIIWGTAFFSWIMDDERVKGLEGK
                     EKRDVALSIYQRVPGGYDKYGKNKKYLEKYYKKRDMIVELFENR"
     gene            complement(4503..5129)
                     /locus_tag="PLG72_08880"
     CDS             complement(4503..5129)
                     /locus_tag="PLG72_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80946.1"
                     /translation="MYNYYRDNPGMMPMQNVGMMPAQNAGMMPAQNANIMPVQNTGLM
                     PMSNMDQMPAANVMPMYNTGLMPSANVMPMYNPCNPMAAMPFQSNQFAEMSTEDLENL
                     YPQTYNVIRPVVESTCDNWMSKYGDKCPSEKEYDSMVSDIYKKVEADVEAVIKNSANP
                     EERQFFGGSRRLLRDFIGAVLIASLIRRRRRPFFGPFYGGFYGGYPFY"
BASE COUNT         1408 a         1236 c         1424 g         1308 t
ORIGIN      
        1 cgggaagctt tgataacgct gtctgtcaca atctgtatca gatcatcgcc ctcgttgatt
       61 ataggcgtcc tgatgcccct taccacggtt cctgtgattc tttccatcca aacactccct
      121 gtacatagtt ttattgttac acttgtatta tatgtgaaat tatgatataa gtaaaataaa
      181 tatccataat atgtactata agtggtgatt atatggcgcc gaaactcgac cagtacagga
      241 ttttttgtga agtggcaaag tgcaggagct tttccagggc tgcaaaaagg ctgtatatga
      301 cccagcctgc cgttagccag gctgtcatga gcctggagag cgaactgggc gtgcggctgt
      361 tcaccaggac ttcgacaggt gtgactctca caaacgacgg ccagttgctt tttgaacatg
      421 tcaactcggc aatcaatctc atacatcagg gcgaaaacaa gctgctggaa tccagggacc
      481 tgaaagtggg cgagatgaag gtcggcgtgg gagatacgat atcaaggtat tttttgctgc
      541 cctatcttga aatattccac agcaggtatc cgaacatcaa gctgaacata gtcaacagga
      601 cgacgacgga actttgcgca ctggtgaaat ccggcgagat agatatcgcg gtatgcaact
      661 ttcctgttaa ggatgatgcc cttgagacca aagaatgcat cgggatacac gatattttcg
      721 tctgcggagc caaatacatc ggccagttcc gggagcctgc cggactcgac gtgattgccg
      781 ggatgccgct gatcctgctg gagcgcaagt cgaactccag gctgtatgtg gaaaagtatc
      841 tgctgtcgca ggggataagg gtaacgcctg aaatagaact gggctcccat gacctgctgc
      901 tagagttcgc cagtttcaat ttcggcatag cgtgcgtcgt ggaggagttt tcccgggaat
      961 atctcagctc gtcgagagtg ttcaggataa agctcacccg tgagataccg ccaagaagca
     1021 tcgggttttg ccacctcaaa agcgttccct tgtcgccggc tgcgcagaaa ctggtcgaag
     1081 tggtgttcag cgaactgaaa tctggaagga cagtgaaagg gtacggccag gaatgaacag
     1141 aaaccggacg gtcgggaaaa gtaagggaaa ccggacatcg gtactgagcc gattcaggac
     1201 ataaaaaacg actccagcga acctttctcg atccaatacc ttcagcactg acccttttcc
     1261 tcaggctttt tcgtcctccg taccggaacc tgcctttcgg tatgctatct ccggatgctt
     1321 tcaccctgca tccccttcga ttcgcatcct ccggtttctt tccgatcctt tggatatagc
     1381 ggattttaat tcacttagtg aaccatttta tccgctcgcc tttccgcata ttatgcggcg
     1441 gccgctgctc catcggtact gttcttaggt attccctgga gtctaatgaa attataacag
     1501 aatacctccg tatgacacaa catgcatctg gctataaaac atgggaatat tttcatctat
     1561 ctcagtattt ttttatctat cagcgatttg ctcaatattt cattttccgt tgacgatttt
     1621 ttgtgcagca tgctgagatc cacgtttttt tcataccata tttatggtaa tattattgca
     1681 ggcaggtgat gtttttggcc ataattacga tttcgaggca ggacggaagc tacggaagcg
     1741 aaattgcggc aaagctgagc gagaaactcg gcatgcggct tattctcaag gatacggtcc
     1801 ttaatgaatg gatgagggaa gtcacaaatg attaccagct caggatgctg acggaaagcc
     1861 cgaaattcta caacaatgta atggactgcg ggcttacata caaggaatat atcgagagaa
     1921 agcttgtgag cgaagcggaa aagtccccgt gcgttattgt cgggatgggg tcgcagatca
     1981 tttttgccgg ccatcccgat gctttgaata tcaggatcat cgcgtccgac ggcacaaggg
     2041 tcgaaaggct caggcaaaag tacggccttg gcgccaggga agcggagcgg ttgatgcagc
     2101 agggcgacag gaggcacagg aggtacatat ccgtgctgta cggggctgat tgggacgatg
     2161 tccgccacta cgacatgatc ctcaggacgg atgacctgac cgtggacatg tgtgtcgaaa
     2221 tcatatccgg aacagctgtt atgaaggata agctgaaagc ctccggacag gccgttcccg
     2281 gcacggaacc tgcaccggct gtgaggcgcg gcgagatccg gttcaagcac ccggccgagg
     2341 aggaattcgc aaagatcctt gatatgtacg ggatagaatg gcagtacgaa cccaggacgt
     2401 ttccggtcgg atgggacgcg gaaggcaacg tgaagatggc gataaaaccg gatttttacc
     2461 tggtgaagtt cgacacatac gtggaaatca ccacgatgga ccagaaatat gtgacgacca
     2521 agaacaaaaa ggtcaggatg ctgcgggaac tgtatcccga catcaatatc aatatcgtgt
     2581 acaagaagga tttccattcc ctgctcaaac gtttcggatt cggcggagag gatgagatag
     2641 atgaatttaa ccggaatgaa aagaatactg ttcagcgaag agatgataaa gaacagggtg
     2701 aaggaactgg gcaggcagat cagtgaggac tacagggaca aacatcccat actgatcagc
     2761 gtgctcaagg cgacaatgta ttttgtggcg gatctcacaa gggagcttga tatccctgta
     2821 aatatagatt tcatgtcgat cggcgtgtac ccgggcatca cgaagaacac cggagtggtc
     2881 aggatcaaca aggacctgga tctcaatatc accggaaggc atgtgcttat ggtggaggat
     2941 ataatcaaca ccggcctcac catcgggtat ctcgtccaga acatggaggc caggaaaccc
     3001 gcaagtatca aaatctgtac cctgctcaac aacccgtcga agcgtctggt gaacatcccg
     3061 ctcgagtatg tagggtttga agtccctgac gtgctgcttg tcgggtatgg gatcgactac
     3121 agccaggagt acaggcatct gccctacata gctgaattca gcgaggaaga gtatctgaaa
     3181 gccaactccg gcggcgtgtg accggacgga tatgggaaat gcttcccgct gaaaaggccg
     3241 taaggctggc agaccgccag acaaatgttc tgctgttatt gtaaacctgt aaatgatata
     3301 attatatgca gccggatatg atgccgcggc agatatgccg tgatatgccg cggacggctg
     3361 cattacttgt ttttgggggt attttgtgtc ttcgaaggcc gttgtcagtt taatagcagc
     3421 cctggcgatc tctgtttcag gtatcgtacg tcctgttgcc gcattagccg cagaggaagg
     3481 agcaaatgcc gcagtgacag gatcaggtaa ctcattgacg gaatcggaca gcgtattgtc
     3541 ggaagaagta aaggcatccg ggtattacgc tgctttggct gcagtttcgc tgttgaaaca
     3601 ggcagggatg atcctcgggg atcctgttgt tgcagcggca gcggctgacg tgaagaaagc
     3661 cgccgataca gcggcgtctt tatcagggct gcctatacag gcaggcgctt cggcggaaga
     3721 tgacgagagc ctgtggtact gtcctggcat cccgatgaaa aaggagcatc agaaactgct
     3781 ctgggagtgc tgcagggaaa gagggctcga ttacatagat atgctggcgc tgatagcgct
     3841 ggagagcaac ttccaggaga agtgttccaa cgggaaatac aagggatact tccagatatc
     3901 atcggatcac ggaacgacac tgtccaggca actgaataca aaaaatgatc cgctggacgg
     3961 tactgtaaac ataatctggg gcacggcttt cttcagctgg ataatggacg acgagagggt
     4021 taagggcctg gaaggcaagg agaagaggga tgtggccctc tcaatatacc agcgcgtgcc
     4081 gggcggctat gacaaatacg gcaagaacaa gaaatatctt gaaaagtatt ataagaagcg
     4141 ggacatgata gtggagctgt ttgaaaacag atagagggtt gcaggcacgc atcgagaaat
     4201 atgttaatgg catttgagat cagcaatttg tgatataagc gaatgaaatg ctatatataa
     4261 aacggcataa aaagggggtc cggatgagcc ccctttttga tactggctta tcgcacaccg
     4321 gacgtgcatt ccggtttccg gtgttgagct ggacagcacc attacagtaa tagctggcag
     4381 tactgtaatg gtcagcaata ttgcagtgcc caatcggcaa aataactgca ggtacagtgt
     4441 ttagctgcaa ctgcagtata tactgccggc atagtgtgtt tctttggcaa cagcaccttt
     4501 actcagtaga acgggtaacc gccgtaaaaa ccgccgtaga atggcccgaa gaacgggcgt
     4561 cttctcctgc ggatcaggct agcgatgagg acagccccta tgaagtccct cagcaggcgc
     4621 ctgctgccgc cgaagaactg cctttcttca ggattggcgc tgttctttat gacagcctcg
     4681 acgtcagcct ccactttctt gtatatgtcc gaaaccatgg aatcatattc cttttcggac
     4741 gggcatttgt ccccatattt gctcatccag ttgtcgcatg tgctttccac tacgggccgt
     4801 atcacgttgt aagtctgcgg atacaggttt tcaagatcct ccgtcgacat ctctgcaaac
     4861 tggttggact ggaacggcat tgccgccatc gggttgcagg gattatacat cggcattacg
     4921 ttggcagacg gcatgaggcc ggtattgtac atcggcatca cgttcgcggc cggcatctgg
     4981 tccatgttcg acatcggcat cagacctgta ttctgaaccg gcattatgtt tgcattctgt
     5041 gccggcatca tacccgcatt ctgcgccggc atcataccca cgttctgcat aggcatcatg
     5101 ccgggattgt cacggtaata attatacatc ggccatcctc ctgttctcat attggaatca
     5161 taaatgtttt cctcgttaca atttatgaat taaatatgag aagggttacg gtttattgct
     5221 ttatgttaat atagatttta cagtcggttt ctgcctgcgg ctcaggacaa acccaaaaac
     5281 actgtccacg gcgcaaaata ttataaagat tactgtaaat cctgcggcaa agttctgata
     5341 gttggtcaaa gccttcgcat cgctccatcc cgtctt
//
LOCUS       DAOSCC010000169         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9722_length_5340_cov_4.349290, whole genome shotgun sequence.
ACCESSION   DAOSCC010000169 DAOSCC010000000
VERSION     DAOSCC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_5340_cov_4.349290"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLG72_08885"
     CDS             <1..641
                     /locus_tag="PLG72_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ80947.1"
                     /translation="SMLQQSLISVSSVFITGMVNRFGADATTAFGASGRIESIVFMPA
                     MSIGLAASALTGQNLGAGNVKRVREVFRWGVIMTAGITLSLSAVILLFPKPLLTLFVH
                     DPEILEIGAGYLGTLGYAYILFAVMFVSNGVINGSGRTLVTMCITVLSVWLVRIPLAA
                     VLSSTGLGIRGIWLAVVIGFAVGLASSLSYYFAGGWKKAAFRPGQAAGGYLE"
     gene            complement(700..3294)
                     /gene="clpB"
                     /locus_tag="PLG72_08890"
     CDS             complement(700..3294)
                     /gene="clpB"
                     /locus_tag="PLG72_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOJ80948.1"
                     /translation="MDIDKFTEKAQQAIGTAQEIALRMNHQQVDGEHLHMALLTQEDG
                     LIPKLISYMGINTELLIRDVQKELDKLPAVYGAGSSGMYATRRLNQILLQAADEAGRF
                     KDEYAGVEHVYLALLKERGTPSAEIFRRNGINRENFLSALGKVRSNQRITSKNPEETY
                     EALARFGRDLADMARQGKLDPVIGRDAEIRRVIRILSRRTKNNPVLIGEPGVGKTAVV
                     EGLAQRILKGDVPEGLKNKTVFALDMGSLIAGAKYRGEFEERLKAVLKEIEKSEGRII
                     LFIDELHNIVGAGKAEGAMDAGNILKPMLARGELRCIGATTLDEYRKYIEKDAALERR
                     FQPVMVEQPDVEDTISILRGLKERFEIHHGVRITDNAIIACAVLSNRYISDRFLPDKA
                     IDLMDEAAAMIRTEIDSMPAELDEISRRIMQLEIERQALKKETDRSSLERLAALEKEI
                     AELKEQSDLAKAQWESEKMALVREKELKQQIEEVKRQIEEAERRYDLEELAVLKHGKL
                     PGLEKQLQAEKEKQQASGRKRLLKEEVTEEEIAEIVSKWTGIPVTKLVENERDKLLNL
                     ESVLHKRVIGQDEAVEAVAGAVLRARSGLKDPRRPIGSFIFIGPTGVGKTELAKALSE
                     ALFDSEDNMVRIDMSEYQEKHSVARLIGAPPGYVGYEEGGQLTEAVRRKPYCVILFDE
                     IEKAHPEVFNTLLQLLDDGRLTDSKGRTVDFKNTVVIMTSNLGSRYLLESIRSGRTDD
                     EEMRESVMAELRSNFRPEFLNRIDEIVMFRPLGRNEIIKIIDLQLADLNRRLADRRIE
                     LRITDAAKEHIADTTYVPEYGARPVKRFLQKELETELGKMIIKDVIHEGSVAVVDAEQ
                     GSLTIRAE"
     gene            complement(3432..4373)
                     /locus_tag="PLG72_08895"
     CDS             complement(3432..4373)
                     /locus_tag="PLG72_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ80949.1"
                     /translation="MKYKDYYQILGVSKNASEDEIKKAYRKLAKKYHPDTHPGDRAAE
                     EKFKEINEAYEVLGDPEKRKKYDQFGSQGQFFHGADFDPSQFGFGNGVHYEFRSGGSG
                     FSDFFDWIFGGGDPFSAFGDSGVFGKRSGSGSHFNRSMKMKGGDTETVLEISIAEGFS
                     GAEKLISLRGANGEKKISLKIPAGIKQGEKIKLAGQGNPGINGGPNGDLYLRIEFRKD
                     PVFELNGLDLEARLQLYPWEAALGAEKPFNTLDGRISVRIPAGVQTDSRIRVAGKGYR
                     DKSGRRGDLFLRAVIVNPPRLNREQLELYEKLSRIQV"
     gene            complement(4569..>5340)
                     /locus_tag="PLG72_08900"
     CDS             complement(4569..>5340)
                     /locus_tag="PLG72_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ80950.1"
                     /translation="TGTTYLICSTGLSDSVDEDTIYDILASGAEPDETAGELVAEALK
                     KEPEEAVSAMVIRIEDANEQYDYGDDMARPIQNRAPSVRVQPRYSGNTAARRRQIPVD
                     VGKIAAILVLVVLATAVILGGYKLWKMLSPQAKVPVEDSVPANNGTEGEDSTPADDTP
                     DGQEQDDDLEPADEPDDAEGDETGQPGTPSGGNSGDSYEIGPDGTTYIVKPGDMLMAI
                     AKKFYGDESKYVLIMKANNIEDPNKIYVNQELKIPPLP"
BASE COUNT          973 a         1619 c         1247 g         1501 t
ORIGIN      
        1 cgtccatgct gcagcaatcg ctgatctcgg tgagctcagt attcatcacc ggcatggtca
       61 accgtttcgg cgccgatgcc accacggcct ttggcgcgtc aggaaggata gaaagcatcg
      121 ttttcatgcc ggcgatgtcc attggcctgg cagcgtcagc gctgaccggc cagaaccttg
      181 gcgcggggaa cgtgaaacgc gtcagggaag ttttcaggtg gggcgtcata atgacagcag
      241 gaataaccct gtcattatcc gcagttatcc ttctgttccc gaagccgctg ctgacactgt
      301 tcgtccacga tcccgaaata cttgagatcg gcgccggtta tctcggaaca ctgggatacg
      361 catatatcct tttcgcagtc atgttcgttt ccaacggtgt cataaacggg tccggaagga
      421 ccctggtgac catgtgcatc accgtattat cagtatggct ggtcaggatc ccgctggccg
      481 ctgtcctctc atcgaccggt ctgggcatca ggggcatttg gctggcagta gttattggat
      541 tcgccgtagg cctggcatca agcctcagct attattttgc cggcggatgg aaaaaagcgg
      601 cgttcaggcc gggacaggcc gcgggcgggt acctggaata gatcccgccc aaatgcggct
      661 ttggctgtgc cacaactaaa gaggcaaggc gacattattt cattccgccc tgatggtcag
      721 ggaaccctgt tctgcgtcca cgaccgccac tgaaccttcg tggatcacat cctttatgat
      781 catctttcca agttcagttt caagttcctt ctgcaggaag cgtttcacag gccttgcgcc
      841 gtattcgggc acataagtgg tgtctgcgat gtgttctttc gcggcatccg ttatgcgcag
      901 ctctatcctc ctgtcggcaa gcctcctgtt gaggtcggca agctgcaggt ctattatctt
      961 tattatttca ttcctgccaa gtggcctgaa cataactatc tcatcgatac ggttgaggaa
     1021 ttcgggcctg aagttgctcc tcagttcagc catgacgctt tccctcattt cctcatcgtc
     1081 tgtccggcct gagcgtatgc tttcgagcag gtatctgctg ccgaggttcg aggtcattat
     1141 gacgacagta ttcttgaaat ccaccgtccg gcccttgctg tcggtcaggc gtccgtcgtc
     1201 aagcagctgc agcagcgtgt tgaatacttc cgggtgcgct ttttcgatct catcgaacag
     1261 gatcacacaa tatggcttcc tccttaccgc ctccgtcagc tggccgccct cttcataacc
     1321 gacatatccg ggaggcgccc ctatcagcct tgctacactg tgcttctcct ggtactccga
     1381 catatcgatc ctgaccatgt tgtcctcgct gtcgaacagc gcctctgata aagcttttgc
     1441 aagttcggtc ttcccgacgc cggtcgggcc tatgaatatg aacgagccga tcggcctgcg
     1501 cggatctttg agtcctgaac gcgcccgcag tacggcacct gcaactgcct ccacagcttc
     1561 gtcctggccg atcactcgtt tatgcagtac gctttccagg ttgagcagtt tgtcgcgctc
     1621 attctccaca agttttgtca ccggtatacc ggtccattta gatacgatct cggcgatctc
     1681 ttcttctgtc acttcttctt tcagcaggcg cttcctgccg gacgcctgct gtttctcctt
     1741 ctcggcctgc agctgttttt caagtccagg cagtttccca tgcttgagca ctgccagttc
     1801 ttccaggtca tacctgcgtt cggcttcctc tatttgacgc ttgacttcct ctatctgctg
     1861 tttcagttcc ttctccctga caagagccat tttctcgctc tcccactggg ctttcgccag
     1921 gtcggactgc tccttcagtt cagcgatctc cttttcgaga gcagccagcc tttcaagcga
     1981 gctcctgtca gtctcctttt tcaatgcctg cctttctatc tcaagctgca tgatcctgcg
     2041 gctgatctca tccagttctg caggcatgct gtctatttcg gtacgtatca tagcggcggc
     2101 ttcatccatc aggtcgatgg ccttgtcggg caggaagcgg tcgcttatgt agcggttcga
     2161 cagcacagcg caggctatta tggcattatc ggttatcctc actccatggt gtatctcgaa
     2221 tctctccttc agccccctga gtatcgatat cgtatcctcg acatcaggct gttcgaccat
     2281 tacaggctga aacctgcgtt caagcgccgc atccttttcg atatacttcc tgtactcatc
     2341 cagcgttgtc gcaccgatgc agcggagttc gcctctcgca agcatcggtt tgagtatgtt
     2401 gccggcatcc atggcgcctt cggccttccc ggcgcctact atgttgtgca gttcatcaat
     2461 gaacaggatt atcctgccct cggatttctc gatctccttc aggaccgcct tgagcctttc
     2521 ttcgaattca cccctgtatt tcgctcctgc tatcagcgaa cccatgtcga gagcaaaaac
     2581 cgtcttgttt ttaagccctt cgggcacatc gcccttcagt atccgctgtg ccaggccttc
     2641 gaccacagcc gttttaccga cgcccggttc acctatcagg acggggttgt ttttagtcct
     2701 ccgggaaagt atcctgatga cccgcctgat ttccgcatcc ctgcctatca cgggatcgag
     2761 ctttccctgg cgcgccatgt ctgccaggtc cctcccgaac ctggccagcg cctcgtatgt
     2821 ctcctccggg ttctttgaag tgatgcgctg gttgctcctc actttcccga gggcggaaag
     2881 gaaattctcc ctgttgatcc cgttcctcct gaaaatttcg gccgacggcg tgcccctctc
     2941 cttaagcagc gccaggtata cgtgttcgac tccggcatat tcgtccttga acctgccggc
     3001 ttcgtccgcg gcctggagaa gtatctggtt gagcctcctg gtcgcataca tccctgacga
     3061 accggcgccg tataccgcgg gaagcttatc gagttccttc tgtacatccc tgatcagcaa
     3121 ttcggtgttg atgcccatat aactgatcag tttcggtata agcccgtcct cctgcgtcag
     3181 gagcgccata tgcagatgct cgccgtccac ctgctggtgg ttcatccgca gggctatctc
     3241 ctgcgcggtc ccgatggcct gctgcgcttt ttcagtaaat ttgtctatat ccatggtcac
     3301 ttgcctcctc tgagaatctt agttaagtca gaatagaatt catagtgttt tgaatagtgt
     3361 caatgcaatt gatctgtatc aaattgcatt gacccactat aatctcttaa tttgcttcac
     3421 attttatgaa ttcagacctg gatcctgctc agcttctcat aaagctcgag ctgctccctg
     3481 ttcagcctcg gcgggttcac gatgaccgcc ctcaggaaaa ggtcgcctct cctgccggat
     3541 ttgtccctgt agcctttccc tgccaccctg atcctgctgt ctgtctgaac accggccggt
     3601 atcctgaccg atatccttcc gtccagcgtg ttgaaaggct tttcggcgcc cagcgcagcc
     3661 tcccacgggt aaagctgcag cctggcttca aggtccagcc cgttcagttc gaatacagga
     3721 tctttcctga actctatgcg cagatacagg tctccgttgg gtccgccgtt gatccccgga
     3781 tttccctgcc ctgccagttt tattttctca ccctgcttta tccctgcagg aatcttcagc
     3841 gagatcttct tctcgccatt ggctcccctg aggcttatca gcttttcagc gccgctgaac
     3901 ccttcggcta tggatatctc cagcaccgtc tccgtatctc cgcccttcat cttcatgctg
     3961 cggttgaaat ggcttccgga tccgctcctt ttcccgaata cgccgctgtc gccaaaagcg
     4021 ctgaagggat caccgccgcc gaagatccag tcaaagaagt cgctgaatcc gctgccgccg
     4081 gatctgaatt cataatgcac cccgtttccg aaaccgaact gtgacggatc gaaatcggcg
     4141 ccatgaaaaa actggccctg gctgccgaac tggtcgtact tctttctctt ttcagggtct
     4201 ccaagcacct catatgcttc gttgatctcc ttgaatttct cttctgcggc cctgtccccg
     4261 ggatgtgtgt caggatggta cttcttcgcc agtttcctgt acgctttctt tatttcatct
     4321 tccgaagcat ttttgcttac ccccaggatc tgataataat ctttatactt catccaacca
     4381 ccttccggtg ctgactttga ctttctttga cctatatatt attatacacg ttcacatgaa
     4441 aaatgcaagc aatatcagaa ctatttcccc ataaacggat aggctcaggt catctgtcca
     4501 atgactttgc cgccgattaa aaaacaggcg acattttcat gtcgcctgtc atgatatccc
     4561 tcgatggatt aaggcagcgg aggtatcttc aactcctggt tcacatagat cttattcgga
     4621 tcttctatat tgttggcttt catgatcaga acatacttgc tttcgtctcc atagaacttc
     4681 tttgctatcg ccatcagcat atcgcccggt ttgacgatat atgtggtgcc gtcaggtcct
     4741 atttcgtagc tgtcgccgct gttgccgccg ctcggtgtac ccggctggcc ggtctcatcg
     4801 ccttcagcgt catccggttc atccgccggc tcgagatcgt catcctgttc ctgtccgtcc
     4861 ggtgtatcat cggccggggt ggaatcttcc ccttcggtac cattgttggc cgggacactg
     4921 tcctcgaccg gcaccttcgc ctgcgggctc aacatcttcc agagcttgta tccgcccaat
     4981 atcaccgctg tagcgaggac caccagtacc agtatggccg ctatcttgcc gacatccacc
     5041 gggatctggc gcctgcgtgc tgcagtattc ccggaatacc tcggctgtac cctgacggat
     5101 ggtgccctgt tctgtatcgg cctggccatg tcgtcgccat aatcgtactg ctcattcgca
     5161 tcctctatcc tgatgaccat tgccgacacg gcttcttcgg gttccttttt cagcgcttca
     5221 gccacaagct cccctgcggt ttcgtcgggc tctgcgcccg atgccagtat atcgtatatc
     5281 gtatcctcat ccacagaatc tgagaggcct gtactgcata tcagatatgt ggtccctgtt
//
LOCUS       DAOSCC010000170         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9830_length_5291_cov_4.640756, whole genome shotgun sequence.
ACCESSION   DAOSCC010000170 DAOSCC010000000
VERSION     DAOSCC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_5291_cov_4.640756"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="purL"
                     /locus_tag="PLG72_08905"
     CDS             complement(<1..1362)
                     /gene="purL"
                     /locus_tag="PLG72_08905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOJ80951.1"
                     /translation="MSKPYEAVGLTEQEYKKIVEYLGREPNELELNMYGVMWSEHCSY
                     KHSRALFRHFPTTGKRVLQGPGENAGIIDIGDNLAIVMKIESHNHPSAVEPYQGAATG
                     VGGIIRDIFAMGARPIAILDSLRFGDIENSERVKYLVDGVVAGIAGYGNCIGIPTVAG
                     EVGFHNSYNGNPLVNAMCVGIIEHDKIFRGNASGVGNSVMYVGAATGRDGMGGASFAS
                     VELTEESEEKKSAVQVGDPFMEKLLLEACLELLETGSIVGLQDLGAAGLTSACCETAA
                     RGEGGMEIDVLKVPRREKGMTPVEVMISESQERMLLIVEKGREDEVKSIVEKWGLHAV
                     TIGKVTGNGRLVVKEGDKIVADVPACSLDSSGAPKYYREYEEPGYYRELKQLDLESIP
                     EPGDYNKTMMDLLTSPNLCSKEWVYRQYDHMVRTSTVVKPGSDAAVMRIRGTKKGIAL
                     TTDC"
     gene            complement(1364..2035)
                     /gene="purQ"
                     /locus_tag="PLG72_08910"
     CDS             complement(1364..2035)
                     /gene="purQ"
                     /locus_tag="PLG72_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOJ80952.1"
                     /translation="MKFGVIVFPGSNCDIDCFHAVKDALGRDVEYIWHDDEELDGFDC
                     IILPGGFSYGDYLRCGAVARFSRIMEAVKAHAASGKLVIGICNGFQILTEAGLLPGAL
                     VRNKNLKFICDTVPLRVETTDTPFTSLYKKGEIINIPIAHGEGNYVVDEETLRKMKEN
                     GQIVFSYVDNPNGSMENIAGVCNEQRNVLGMMPHPERVCEEILGNTDGLRLFESIVTA
                     LEGRR"
     gene            complement(2104..2343)
                     /gene="purS"
                     /locus_tag="PLG72_08915"
     CDS             complement(2104..2343)
                     /gene="purS"
                     /locus_tag="PLG72_08915"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOJ80953.1"
                     /translation="MKAKVYVTLKKSIADPQGNAIQNALRKLGYTNVEEVRIGKLIEL
                     DLGDITADEAQAQLVKMCEGLLANTVIEEYKYELC"
     gene            complement(2585..3865)
                     /locus_tag="PLG72_08920"
     CDS             complement(2585..3865)
                     /locus_tag="PLG72_08920"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOJ80954.1"
                     /translation="MLTAIVGINWGDEGKGRMVDLLGSQYDIIVRYQGGNNAGHTVVN
                     EKGKFILNLLPSGILRNDTVNVMGPGMVVDIAHLCKEIKGLSEKGIKITPENLKISQG
                     ATICLPYHVRQDILEEERLADKKYGSTRRGIAPVYADKYMKKGIRMSDLLFPDTLREK
                     LEGIVEWKNLMICGGYRDQAVDTDEMLEWLKVHGEPIIPFICDVTEYLENAVRENKNI
                     LFEAQLGALRDIDFGIYPYTTSSTTLASFATTGAGIPGAKLDNVIGIMKAYSSCVGEG
                     PFVAEFFGEEGSRLREAGSEYGAATGRPRRVGAFDIPASRYGIKVQGADEIALTKLDV
                     LSYMDKVPVCIAYEIDGERTDRFPTGDRLLRAKPVIEWLDGWKCDISSCRRKEDLPAE
                     AAAYIRYIEEKTECRIRYVSVGAERDAYIDMDMA"
     gene            complement(3957..>5291)
                     /gene="purB"
                     /locus_tag="PLG72_08925"
     CDS             complement(3957..>5291)
                     /gene="purB"
                     /locus_tag="PLG72_08925"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOJ80955.1"
                     /translation="TWRKLWVALAESQKELGLDISDRQIEAMKEHIYDINYEVAEQRE
                     KEVRHDVMAHIYAYGVQCPEARPIIHLGATSSYVGDNTDLILMKEALVQIRSSLAGVI
                     VELARFALKHKSLPTLAYTHFQPAQPTTLGKRATLWMQDLVSDMDALEFVTSQIKMLG
                     CRGATGTAASFLELFDGDAEKVERLEKLIAQKMGFDGIFDVSGQTYPRKLDYNVLSIL
                     SGIAQSAWKFSNDVRLMSHLKEVDEPFEDKQVGSSAMAYKRNPMRSERISSLSRHVIA
                     LAQDAAMTASTQWFERTLDDSAARRIYIPEAFLATDGILSLYRNVIGNMRVYPEMMRR
                     HLEEELPFMATENILMHCVKKGGDRQLLHEAIREIAVSTARNIKETGARNDLIERILA
                     DERFNITREEMENLLDAGKFTGLAEKQAETYAKKILDRFGENLVSEDAEINV"
BASE COUNT         1040 a         1547 c         1222 g         1482 t
ORIGIN      
        1 gcagtccgtg gtcaaagcga tacctttttt agttccccta atccgcatca cggctgcatc
       61 cgagccgggt tttaccacgg tacttgtcct taccatgtgg tcatactgcc tgtacaccca
      121 ttccttgctg caaaggttgg gggaagtcaa aaggtccatc atggttttgt tgtaatcgcc
      181 gggctccggt atggattcca ggtcaagctg cttcagttcc cggtaatatc caggttcctc
      241 gtattccctg taatacttgg gcgcgcctga agagtccagg ctgcaggcag gtacatctgc
      301 aactattttg tcgccttcct tgacgaccag cctgccgttg ccggtaacct ttcctatcgt
      361 tacggcatgg aggccccatt tttcaactat gctctttact tcgtcctccc tgcctttttc
      421 gacgatcagg agcatcctct cctgcgattc ggagatcatg acttcaacgg gcgtcattcc
      481 tttttcacgg cgggggacct tcagcacgtc gatctccatc ccgccctcgc ccctggcggc
      541 agtctcacag catgccgacg tcaggccggc ggcgccgaga tcctggagtc cgactatcga
      601 tcctgtttcc aacagttcca ggcaggcctc cagcagcagt ttctccataa aggggtctcc
      661 cacctgtaca gcggactttt tctcttccga ttcctcggtc agttcaacag aggcaaagct
      721 tgctccgccc ataccgtccc tgccggtggc cgcgccgaca tacattacgg agttgccgac
      781 tcccgaggcg ttgcccctga agatcttgtc atgctcgatt atgccgacgc acatggcgtt
      841 gaccagcgga ttcccgttgt agctgttgtg gaaacctacc tcgccggcaa cggtcgggat
      901 gccgatacag ttgccgtacc ctgcaatacc tgcaacaacg ccgtccacca agtatttgac
      961 cctttcgctg ttctctatgt cgccgaagcg gagggagtcg agtattgcaa ttggccttgc
     1021 acccattgcg aagatgtccc ttatgattcc gcctacgccg gtggcggcgc cctggtaggg
     1081 ctctacggcg gaaggatggt tatggctttc tattttcatg acgattgcca ggttatcacc
     1141 gatatcgata attcctgcgt tttctcccgg tccctgcagg acccttttgc ctgttgtcgg
     1201 gaagtgcctg aacaatgccc gcgaatgctt gtagctgcaa tgctccgacc acatgactcc
     1261 atacatgttg agttcaagtt cattgggctc cctgcccagg tattccacga tttttttgta
     1321 ttcctgttcc gtcagtccta ctgcttcata cggtttgctc atgttaccgc ctcccttcaa
     1381 gtgccgttac gatggattca aacagcctca ggccgtcggt attgcccagt atctcctcgc
     1441 agaccctctc ggggtggggc atcataccca gtacatttct ttgctcgttg catactcccg
     1501 ctatattctc catggacccg ttgggattgt ccacataact gaaaactatc tgtccgtttt
     1561 ccttcatttt cctgagggtc tcttcatcca ccacgtaatt tccttcccca tgggcaatgg
     1621 ggatgtttat gatctcgcct tttttgtaca ggcttgtaaa tggggtatcg gtggtctcca
     1681 cccttaaagg taccgtatcg catatgaatt tcaggttttt attcctgacg agagccccgg
     1741 gcagaagccc cgcctctgtc aatatctgga atccgttgca tatcccgatg accagcttgc
     1801 cgctggcggc atgggccttt acggcttcca ttatccttga aaaccttgca acggcgccgc
     1861 atcttagata atctccgtag gaaaagcccc ccggaagtat gatgcagtca aagccgtcaa
     1921 gctcttcatc atcgtgccat atatactcga catcgcggcc cagtgcgtcc ttcaccgcgt
     1981 gaaagcagtc gatgtcacag ttggaccccg ggaaaactat tacgccgaat ttcatcctga
     2041 tgacctcctc ctgactcctg atcgatctcc tctgattgag tattctgact gtgtcatccc
     2101 ctcttaacag agttcatatt tatattcttc aatgacagtg ttggccaaca ggccttcgca
     2161 catttttacc agctgtgcct gcgcctcatc cgcggtgata tccccgagat caagttcgat
     2221 cagcttacct atccgaactt cttcgacatt cgtatagcca agcttacgaa gagcattttg
     2281 tatcgcgttg ccctgcggat ccgctatgct ctttttcagt gtcacgtata cctttgcttt
     2341 catcctgact cctcctgaca tatcattgat ctgatcttaa taacagtctt catccggctt
     2401 ccagccgtcc attatatcta atatacctgt ttgtccggat atttcacgct tgtccggaaa
     2461 gcttcgttcc atgccctgcc ctttgcatgc ggcaggcatg gggtactgaa accacttcac
     2521 aatcactgat cgccgattaa ataaaatacg gacggataag tgctctgcaa aagagttaac
     2581 ctgttcaggc catatccata tcaatgtatg cgtcccgctc tgctcccacc gatacgtaac
     2641 ggatcctgca ttccgtcttt tcctcgatgt atcttatata agcggctgct tcagcgggca
     2701 gatcttcttt cctgcggcag gatgaaatgt cgcatttcca gccgtccagc cactcaataa
     2761 caggtttggc cctgagcagc ctgtctcctg tcgggaacct gtccgtcctt tccccgtcga
     2821 tctcataagc gatacagaca ggtactttat ccatgtatga cagcacgtcc agctttgtga
     2881 gggcgatctc gtccgcaccc tggaccttta tgccgtagcg tgatgcagga atatcgaacg
     2941 ctcccaccct gcgcggcctt ccggtcgctg cgccgtattc gcttccggct tccctgagcc
     3001 ggctgccttc ttctccaaag aattccgcga caaacgggcc ttctccgacg caactggaat
     3061 aagccttcat tattcctata acgttatcca gcttcgcccc cggaatgcct gcccctgtgg
     3121 tcgcaaacga agcaagcgtg gtggaggacg tcgtataggg atagatgccg aaatctatgt
     3181 ccctgagtgc gccaagctgc gcttcaaaca atatattttt gttctccctg accgcatttt
     3241 cgagatactc cgtaacgtcg cagataaagg gtatgatagg ttcgccatgg accttcagcc
     3301 actccagcat ctcatcggta tcaacggcct ggtcgcggta tccgccgcag atcattaagt
     3361 ttttccattc cacaatacct tcaagttttt ccctcaaagt atccggaaaa agcagatcgc
     3421 tcatacgtat gcctttcttc atgtacttgt cagcgtacac aggcgcgatg ccgcgtcttg
     3481 ttgaaccgta ttttttgtcg gccagccgtt cctcctccag tatatcctgg cgtacatggt
     3541 acggaaggca tatcgttgcc ccctggctga tttttaaatt ttcgggagtg atctttatcc
     3601 ctttttcaga aagtcctttt atttctttgc agagatgagc aatgtctacc accatgccag
     3661 gtcccatgac atttacggta tcattgcgca ggatccccga cggaagcagg ttcagtatga
     3721 acttcccttt ttcgttcaca acggtgtggc ctgcattgtt cccgccctgg taccgcacta
     3781 tgatatcgta ttgtgaacca agcaggtcca ccattcttcc ttttccttca tctccccagt
     3841 ttattccgac tattgccgtt aacatggcta aaccgcctcc cttttctcag tcatgggtct
     3901 tgggatttcc gaaagccatg gtcccgtcag catgcccatg gctctgactg attggtctaa
     3961 acatttattt cagcatcttc tgaaactaag ttttccccga accggtccag gattttcttc
     4021 gcgtaagttt cggcctgttt ctccgccagc ccggtgaatt tccccgcgtc gagcaggttc
     4081 tccatctctt cccgtgttat gttgaacctt tcgtccgcca ggatcctttc gatcaggtcg
     4141 ttccttgccc cggtttcctt gatgttcctg gctgtcgaca cggcgatctc cctgatcgcc
     4201 tcgtgcagca gctgcctgtc gccgcctttc tttacacagt gcatcaggat attttcagtc
     4261 gccatgaatg gcagttcttc ttcaagatgt cttctcatca tttccgggta gaccctcata
     4321 ttgccaatca cattcctgta cagtgaaagt atcccgtccg tcgcaaggaa tgcttcaggt
     4381 atatagatgc ggcgtgctgc cgaatcgtca agggtgcgct cgaaccactg cgtcgatgcc
     4441 gtcatggcgg cgtcctgggc cagtgcgatc acatgccttg aaagtgaaga tatgcgctcg
     4501 ctccgcatgg ggttacgctt gtatgccatt gccgacgagc ctacctgttt gtcttcgaaa
     4561 ggttcgtcca cttctttcag gtgtgacatg agacggacgt cgttcgagaa tttccacgcg
     4621 ctctgcgcga tgcctgacag gatcgaaagc acgttgtaat ccagcttgcg ggggtaagtc
     4681 tgcccgctta cgtcaaaaat cccgtcaaag cccatcttct gtgcgatcag cttttcaagg
     4741 cgctcgactt tttcggcgtc gccgtcgaaa agttcgagga aactcgccgc ggtaccggtg
     4801 gcgccgcggc acccgagcat ttttatctgc gacgttacga attcaagcgc gtccatgtcg
     4861 cttaccaggt cctgcatcca cagggtcgcc cgcttgccga gggtagtggg ctgcgcaggc
     4921 tgaaaatgcg tgtatgcaag cgtagggagt gatttatgct tcagggcgaa tcgtgcaagc
     4981 tcgactatga ccccggcaag ggacgatctt atctgaacaa gggcttcctt catcaggatc
     5041 aggtccgtgt tgtcaccgac atagctcgat gtcgcgccaa gatggattat cggccttgcc
     5101 tccggacact ggactccgta agcgtagata tgggccatga catcgtggcg cacttctttc
     5161 tcgcgctgct cggcgacttc gtagtttata tcatatatgt gttccttcat tgcctctatc
     5221 tgccggtcgg agatatcaag accgagttcc ttctggcttt ccgcaagggc tacccacagt
     5281 tttctccagg t
//
LOCUS       DAOSCC010000171         5288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9840_length_5288_cov_4.804892, whole genome shotgun sequence.
ACCESSION   DAOSCC010000171 DAOSCC010000000
VERSION     DAOSCC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9840_length_5288_cov_4.804892"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLG72_08930"
     CDS             complement(<1..1101)
                     /locus_tag="PLG72_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOJ80956.1"
                     /translation="MRKKAKTGLSNYRIIVVDDEIGIIDSLSVILRRSGYDFTGVTDP
                     LEAVEKVRNEKYDLMILDYLMYPVHGDKVVEMIREFDKEIHILLLTGHNELAPPLETI
                     KALDIQGYCEKSDRFDQLLLWVESGIKSISQMRTIRKFRDGLNEILRAVPKIYSLQPI
                     DTILEDILQELMPLVDGENAFILVDDITDMSEEGHKSIFKGIGKYRATINHFMEMLSP
                     NLMEYIGYARITKQRVELEEGVILPLNNEKSQAIGVIYVEGANRDDGIELLEIYTNQA
                     ASSINNAFLHSLVNMKNEELSRTYDQLKLRYMDTIEALRQAVDARDIYTRGHSDRVAL
                     YALEIGKAIGLSPEELETLRIGGIFHDIGKIGT"
     gene            complement(1098..2999)
                     /locus_tag="PLG72_08935"
     CDS             complement(1098..2999)
                     /locus_tag="PLG72_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ80957.1"
                     /translation="MLDYPPYSINNDFQIKVAGIHYTWQYVLIVLQIIVFSDIFILVR
                     LKKIDRLNEYLEKNDKVEIEKIRIRCHTFPYLLYAVLVIVPALLSSVVLLAIGTNPVL
                     VYKICSIALTFTTVIALITHVFASKIMAGILYDISFTHRIFGRRIGIRTKMYLQILPA
                     FLAAILFTSLVCYSRHVTEKGNIIFEKYRSELVRSFSGRAKPSREEISAILQTIADEG
                     HTPFLIDPEGNVEPEAIAQNNFFITYLNDIAFDRTGGHVYDYYGVDSQAAAVRVYTYD
                     GIWTAGIKYDVTSPGTIELLGVSLLILIVLYASVLGYISKSMADDISRVAVSLDDIVH
                     GENVDLDRKLPVTSNDEIGDLVVAFNKILELAKANIEAIEQKQTILIERERLASLGQL
                     IGGIAHNLRTPIMSISGAIEALKDLVYEYRDSIGDRSVNEQDHMEIAGEMLTWLDKMR
                     PYCAYMSDVISAVKGQAVQMTASGTVRFTVDEAVKRVELLLKHELKRHHCVLNIMRNI
                     DMRTEIRGEVSNLVQVLDNIIINAMQAYDGQAGTIDMEIVRSGDNVEFIIRDYGKGIP
                     QNVANRLFREMITTKGKKGTGLGLYMSYSTIKGRFGGSITFTSKEGCGTTFFISIPCI
                     TNSGQEEYK"
     gene            complement(3032..>5288)
                     /locus_tag="PLG72_08940"
     CDS             complement(3032..>5288)
                     /locus_tag="PLG72_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOJ80958.1"
                     /translation="AVLKCGAVFLPVDPMYPKERIRYMLEDAGPRAVLYDDGGTMEYM
                     DCGSRWINAAEPASEEDLDPGIYPDGSSLAYIIYTSGSTGNPKGAMITNKGLVNYIVW
                     ADKVYVRGDDMTFALHSSLSFDLTITSLFTPLISGSSIAIYEDGGPEPPIFRVFAENR
                     ADIVKLTPSHLSLIKDMDNSRSRVKRLIVGGEDLRTELARQVHDSFGGNVEIYNEYGP
                     TETVVGCMIYKYDRSGDNGVSVPIGRPADNVRIYILDRYLNPVPIGTPGELCIGGDGV
                     CAGYLNNPGLTAKKFIEDPFIPGERIYMTGDLARWFPKGDVEYLGRIDDQVKIRGYRV
                     EPGEIETQLMSHSGVSSAVVLVYDDPNGRKSLCAYVKANGGTDENELRIYLAKRLPSW
                     MVPSCFIFVDEIPLTPNGKVDRHRLPKPEDKAGQNGYTAPSNETERIIAEAVREITGI
                     EKVGACDSFDDLGIDSLDIIRIQARLLKYGWKLTTQDFYEYPSIRLLSMKIAGKTGER
                     GGPGAGNDNGIMKFPSVSFGNVSYEGDVLITGATGFLGAHLLKEVISGSDADVYCLVR
                     GDGLENAEKRLFSKLEYYFGYDFIKTAGNRIHTVRGDFSIEKFGLADDEYRNLGSRVR
                     RIIHSGALVKHYGDSGLFERTNCYGTEQAADFAEAFGLQFSHISTVSVAGIYDGQSKK
                     AGEFFENDIYMGRFPEDNVYIKSKIMAERMIVKRVLNNKLNASIFRVGNLTGRFSDGV
                     FQQNRNENLPS"
BASE COUNT         1126 a         1492 c         1189 g         1481 t
ORIGIN      
        1 cgtccctatt ttcccgatat cgtggaaaat accgcctatc ctcagcgtct caagctcctc
       61 cggggacagt cctatagctt tccctatctc aagggcatac agcgcaaccc tgtccgaatg
      121 gcccctggta tagatatccc tggcatccac agcctgcctc agcgcttcga tggtatccat
      181 gtatctgagc ttgagctggt cgtaggtcct gctcagttct tcattcttca tattgaccag
      241 cgaatgcagg aatgcattgt tgatggacga ggcggcctgg ttcgtgtata tctcgagaag
      301 ttctatgcca tcgtccctgt tagccccttc aacgtaaatc acgcctatcg cctggctctt
      361 ttcgttgttc aggggcagga tgaccccttc ctccagttcg accctctgtt tcgttatcct
      421 ggcgtatcct atgtattcca taaggttcgg gctgagcatc tccatgaaat gatttatcgt
      481 agccctgtat ttgcctatcc ctttaaagat gctcttatgg ccttcttccg acatgtccgt
      541 gatgtcgtcc accaggatga acgcattctc gccgtccacc aggggcatca gttcctgcag
      601 tatgtcttcg aggatcgtat cgatcggctg cagcgagtat atcttcggaa cagcccgcaa
      661 tatctcgttc aggccgtccc tgaatttcct gattgtcctc atctgggaaa tcgatttgat
      721 gcccgattcg acccacagca aaagctggtc gaaacggtcg cttttttcac aatagccctg
      781 tatatccagg gccttaatgg tctccagcgg cggagcaagc tcattgtgtc ccgtcagcag
      841 caatatatgt atttctttgt cgaattccct tatcatctcc actaccttgt cgccgtggac
      901 cggatacatc aggtaatcaa gtatcataag gtcgtatttc tcgttcctga ccttttccac
      961 agcttcgagc ggatctgtga cgcctgtgaa gtcataaccg ctccgtctca ggatcaccga
     1021 cagcgaatcg attatcccta tctcgtcgtc tacaactatt atcctgtaat tcgacagacc
     1081 tgttttcgct ttctttctca tttgtattcc tcctgaccgc tgttggttat acatggtatg
     1141 gatatgaaga atgtagtacc gcagccttcc ttgctcgtga acgtgatgct tcccccgaag
     1201 cggcccttga tggtggaata tgacatgtac aggcccagtc ctgtaccttt cttgcccttt
     1261 gtggttatca tttccctgaa aagcctgttt gcaacgttct ggggtatccc tttcccgtaa
     1321 tccctgatga tgaattcaac attgtccccg cttctgacta tttccatatc tatagtaccg
     1381 gcttgtccgt cgtacgcctg cattgcgttt atgataatgt tgtcgagcac ctgcacaagg
     1441 ttgcttactt ctcctcttat ctctgtcctc atatcgatat tgcgcatgat attgaggaca
     1501 cagtggtgtc tcttcaactc atgcttcagc agcagttcga cccttttaac cgcttcatcc
     1561 acagtgaacc ttacggtacc ggacgccgtc atctgcacag cctggccctt gacggccgaa
     1621 atgacgtcgg acatgtaagc gcagtaaggt ctcatcttgt cgagccatgt aagcatttca
     1681 ccggctattt ccatgtggtc ctgttcatta acgctccggt cgcctatcga atccctgtac
     1741 tcataaacca ggtccttgag cgcctcgatc gctcctgaaa tggacatgat cggagtcctc
     1801 aggttgtggg ctatgcctcc tatcaactgg ccaagtgatg cgagccgctc ccgttcgatc
     1861 agtatcgtct gtttttgttc tatggcctca atattcgctt ttgcaagttc aagtatctta
     1921 ttgaacgcaa ccaccaggtc gcctatctca tcattcgacg ttacaggcag tttgcggtca
     1981 aggtccacgt tttcgccgtg tactatatcg tcaaggctga cagccacccg agagatgtca
     2041 tcggccatcg acttcgatat atagcccagc actgaagcgt ataagacgat cagtatcagg
     2101 aggctgacac ccagaagttc tatcgtgccc ggtgaagtca catcatattt tatccctgcc
     2161 gtccagatcc cgtcgtaagt gtataccctg accgcagcgg cctgggaatc gacgccataa
     2221 tagtcgtaaa catgtccgcc ggtcctgtca aaagcgatat cgttcaggta cgtgatgaaa
     2281 aaattgtttt gtgctatcgc ttccggttcc acgttccctt ccggatcgat caggaaaggc
     2341 gtgtgccctt cgtccgcaat cgtctgcagt atcgcggata tctcctccct gctcggcttt
     2401 gcccgcccgc tgaaactcct gacaagctcg gagcggtatt tttcaaatat gatgttccct
     2461 ttttcggtta cgtgcctgga atagcatacc agtgacgtga aaaggatcgc cgcgagaaat
     2521 gccggcagta tctgcaggta catttttgtc cttatcccga tcctccggcc gaatatcctg
     2581 tgagtaaacg agatatcgta cagtatcccc gccattattt tgctcgcgaa cacatgggtt
     2641 atcaatgcga taacggtagt aaacgtcaaa gcgatgctgc atatcttgta taccaggaca
     2701 ggattggtcc cgatcgccag cagaacgaca gacgaaagca gggcaggaac gatgaccagc
     2761 accgcataca gcaaataagg gaatgtgtgg caacgtatac ggatcttttc gatctcgacc
     2821 ttgtcatttt tctcaaggta ctcgttcagc ctgtcgattt ttttcagcct gaccagaatg
     2881 aaaatatcgc tgaaaacaat gatttgcaga acgatcagga catactgcca tgtatagtga
     2941 atccctgcaa ctttgatctg gaaatcgttg tttatcgagt aaggagggta atccagcacc
     3001 tgcggcagca gaaaatagaa aactgcgcct attatgacgg caggttctcg ttcctgttct
     3061 gctggaacac gccgtccgaa aaccttcctg tcaggttccc gaccctgaaa atgctggcgt
     3121 tcagtttgtt gttcagtacc ctcttcacga tcatcctttc agccatgatc ttgcttttta
     3181 tgtaaacgtt atcttcgggg aaccgaccca tgtatatgtc gttctcaaag aattcaccgg
     3241 cctttttgga ttgtccgtca taaattcccg ccacgctgac agtcgatata tggctgaact
     3301 ggagaccgaa cgcttccgca aaatccgccg cctgttcagt cccatagcaa tttgtccttt
     3361 caaacagacc gctgtcgccg taatgcttta ccagagctcc tgaatgtata atacgcctga
     3421 ccctgctccc caggttcctg tattcgtcgt cggcaagtcc gaatttttca atggagaaat
     3481 ctccccttac ggtatgtatc ctgtttccgg ctgtttttat gaaatcgtac ccgaaataat
     3541 attcaagttt tgaaaacagc cttttctccg cgttttccag gccatctccc cttacgaggc
     3601 agtatacgtc ggcgtcagaa ccgcttataa cttcttttag caggtgcgct ccaagaaaac
     3661 ccgtcgcgcc ggtaatcaga acatcgcctt cgtaagagac attaccgaag gatacagacg
     3721 ggaatttcat tatcccgtta tcattccccg ctcccggtcc gcctctttct ccggttttgc
     3781 cggctatttt catggaaagc agcctgatac tcgggtactc gtagaaatcc tgggtggtca
     3841 gtttccagcc atacttcagg agccttgcct ggatccttat tatatccagc gaatctatcc
     3901 caaggtcgtc aaaactgtca caggctccga ctttttcaat ccctgttatt tcccgtaccg
     3961 cttcggcgat gatcctttcc gtttcatttg aaggcgccgt gtaaccgttt tgcccggctt
     4021 tgtcctccgg tttcggcagc ctgtgcctgt ccacctttcc gttgggcgtc agcggtatct
     4081 cgtcgacaaa aatgaaacag gacgggacca tccaggaagg aagccttttt gccaggtaga
     4141 tgcgcagttc gttctcatcc gtgccgccat ttgcttttac ataggcgcac aaagacttcc
     4201 tgccgttcgg atcatcatag acaagtacca cggccgagct taccccgctg tggctcatca
     4261 gctgcgtttc gatctctcct ggctccaccc tgtaacctct tatcttcacc tggtcatcga
     4321 tcctgccgag gtactccacg tctcccttcg ggaaccaccg cgccaggtct ccggtcatgt
     4381 atatcctttc gccaggaatg aaaggatcct cgatgaattt cttcgcggtc agcccgggat
     4441 tgttcaggta accggcgcac acgccgtccc cgccgatgca gagctctccg ggagtcccga
     4501 tgggcacggg attcaggtac ctgtccagta tgtagatcct cacattgtcc gcaggcctgc
     4561 ctattggcac tgatacgccg ttgtccccgg accggtcgta tttgtagatc atgcagccta
     4621 cgaccgtttc agtgggcccg tattcattgt aaatctccac atttccgcca aagctgtcat
     4681 gcacctgtct tgccagttcg gtcctgagat cttctccgcc tactatcagc cttttcaccc
     4741 ttgaccggga gttgtccata tcttttatca ggctcaggtg cgacggcgtc agtttgacga
     4801 tatcggccct gttttccgca aaaactctga atatgggcgg ttcagggccg ccgtcctcat
     4861 agatcgcgat cgaactgccg gatatcagcg gggtgaacag cgatgttatt gtaaggtcga
     4921 aagacaatga cgagtgcagc gcgaaggtca tatcgtctcc cctgacatag accttgtccg
     4981 cccacactat gtagttgaca agccctttat tggttatcat cgctcctttc ggattgccgg
     5041 tggagcctga ggtatagatg atatatgcaa ggctgctgcc gtccggatat attcccggat
     5101 ccaggtcctc ttcactggca ggttctgccg cgttaatcca tctgctcccg caatccatgt
     5161 attccatggt gccgccgtcg tcatacagta cggccctggg cccggcatcc tccagcatgt
     5221 accttatcct ctcttttgga tacatcggat cgacaggcag gaataccgcg ccgcacttca
     5281 ggacagcg
//
LOCUS       DAOSCC010000172         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_9975_length_5231_cov_4.886399, whole genome shotgun sequence.
ACCESSION   DAOSCC010000172 DAOSCC010000000
VERSION     DAOSCC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9975_length_5231_cov_4.886399"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLG72_08945"
     CDS             <1..250
                     /locus_tag="PLG72_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOJ80959.1"
                     /translation="SSTKSMTGHLLGAAGAIEAIITVMAIKDGIIPPTINLVNPDPEC
                     DLDYVPNEARKADIRYALSNALGFGGHNAALVFKKFTG"
     gene            459..1181
                     /gene="rnc"
                     /locus_tag="PLG72_08950"
     CDS             459..1181
                     /gene="rnc"
                     /locus_tag="PLG72_08950"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOJ80960.1"
                     /translation="MLDKIITENMDRLKGLEEVIDYTFRDPGNLVLALTHSSYANEKK
                     APDLKSNERLEFLGDSVLNIITSDYIYRNYPELPEGEMTRTRAAIVCEASLMKCAEKI
                     RLGEYLFLGKGEENTGGRNRASILSDAFEALIGAIYIDGGIDEARRFVLRYMKEIYPD
                     IDGKDIFRDYKTMFQEIIQKQTDQRISYRILDETGPDHDKTFTAQVLVGDKPYGEGIG
                     RSKKEAEQNAAKDAISNMKVGK"
     gene            1178..2215
                     /locus_tag="PLG72_08955"
     CDS             1178..2215
                     /locus_tag="PLG72_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ80961.1"
                     /translation="MSKKHVIIPIFVPHKGCPNDCIFCNQKKISGQLEEMTPDKIHET
                     VKEHLGTISQDAFVEIAFYGGSFTAIDKVLQEEFLKHAWSYIEAGKVSEIRVSTRPDR
                     IDTEILEMLKKYGVRTIELGVQSLDDEVLRASLRGHDSAVVFRSAKLIKDMGFRLGIQ
                     IMTGLPGDTREKCMETARKVVSMSPDVVRIYPVLVIKGTGLERLMEKGDYRPQTLEEA
                     VDLCAELLRLFEDNGIDVIRVGLQPTENIREGAGSDVAAGPVHPAFRQLAQARLSLKR
                     IEEIIDSQHLSDAQELIICTGPSNISDVVGQKRSNIESLKSKYGFADVRVMPDPKLSR
                     EIISRAISSAD"
     gene            2700..>5231
                     /gene="smc"
                     /locus_tag="PLG72_08960"
     CDS             2700..>5231
                     /gene="smc"
                     /locus_tag="PLG72_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOJ80962.1"
                     /translation="MYLKRLELQGFKSFAEKIRLDFNGGITAVVGPNGSGKSNIADAI
                     RWVLGEQSIKTLRGSKMEDVIFAGTEHRKPLGFAEVTIVLDNSDSTLPIEYDEVAITR
                     RVYRSGESEYLINKTNCRLKDITELLLDTGIGKDGYSVIGQGRIDEILSTRSEDRRQI
                     FEEASGIMKYKVRKQEAEKKLELTRQNLERINDIITELEAQLEPLREQSEKARRFLDL
                     REELKVLDVNVYLDNMKKSRERLETLEQQLKTVQDQINDENKKLEEITNSNREKTDLL
                     KSLDEKLEAAKQVYYETEGNLERCNSEISLSKEKIANLMQNIERLDAEKAELNEKIEA
                     LESEEANKQNRLNYLEKQLEDYSTKLAAYEKQMEELLKTLDEEERKIELLKSEIMDKM
                     DIYSDKKLQISNLRTHIENMQKRQRSIDTDVYQNIVEKDREKMKKEELAESIRKAEKL
                     IKEHKEKISALSGERGETDRMLSELRAKQSKINSDIQFKSSRVRTLREMEQRLEGYNR
                     SVREVLQACHASPQFGKGIHGALAQLIEVDRKYETAIEISLGGALQNIVTQTEEDAKK
                     AIEFLKKNKLGRATFLPISSVEGKGFDSQTLGRIKQCEGFCGIASDLVKHDPLYNGII
                     RSLLGKVVVIDDLDNGIKMARKFGYSFRIVTLEGDLLNTGGAISGGSIENRGTGILTR
                     NREIGELDEELKALKQQESEVNNDIRLLTEDISRIEAEIASIEKEIKDNELIKIRDES
                     HLAQTDENISKLSARIDMLRQEKEQLERQIKETEDELVKYELELLQTEQEINEAKKTV
                     SEFQEKHKEDQAVRDALHQDITDFKISVNSILESIAGVKENADRLR"
BASE COUNT         1685 a         1136 c         1388 g         1022 t
ORIGIN      
        1 aagttcaacg aaatcgatga caggccattt acttggagcg gcaggagcca tagaagcaat
       61 cattacggtc atggcgatta aggatggcat catcccgcca accatcaacc ttgtgaatcc
      121 tgatcccgaa tgcgatctgg actatgttcc aaatgaggcc agaaaagcgg atatcaggta
      181 cgctctgtcg aatgcccttg gcttcggcgg ccataatgcc gcattggtgt tcaaaaagtt
      241 caccggttga atatgatgtc tgtaatgaca ccaataataa ctaagcggaa aagagtttgc
      301 gtgaaggcaa gatatggggt aaaatttgta aaggcgggaa tgcggtttcc tgcctttacg
      361 tttttagaca acaatcccgc tgctggataa aacacgatca gataaaccag gcgcctgaaa
      421 acgaacggcg gtatcatgat ggaacgggga gactgcagat gcttgataaa atcataacag
      481 aaaatatgga cagactgaaa ggactggaag aagttataga ctacacgttc agggatcccg
      541 gcaaccttgt gctggccctg actcacagtt cgtatgccaa cgaaaagaaa gcacccgatc
      601 tcaaaagcaa tgagaggctt gaattcctgg gagattcggt attgaacata atcaccagcg
      661 attatatata caggaactat cccgaactgc ctgaaggcga aatgaccagg acaagggctg
      721 caatcgtctg tgaagcgtca ctcatgaagt gtgctgaaaa aatccggctt ggcgaatacc
      781 tgttcctcgg gaagggtgaa gaaaacaccg gaggcagaaa ccgggcatcg atactgtcag
      841 acgctttcga ggcgttgata ggagccattt atatagacgg gggcatagat gaagcaagaa
      901 gattcgtatt gagatacatg aaggaaatat atccggatat cgacgggaag gatatattca
      961 gggattataa aaccatgttc caggaaatca tccagaagca gaccgatcag aggatatcat
     1021 acaggatact tgacgaaacc ggcccggacc atgacaagac attcacggcg caggtcctcg
     1081 taggagacaa gccttacggc gaaggtatcg ggcgcagcaa aaaggaagcg gaacagaacg
     1141 ctgcaaaaga tgcgatcagc aatatgaagg tggggaaatg agcaaaaaac atgtgatcat
     1201 acccatattc gtcccgcata agggatgccc caacgactgc atattctgca accagaaaaa
     1261 gataagcggc cagcttgagg aaatgacccc ggacaaaatt catgaaacgg ttaaggagca
     1321 tcttggtaca ataagccagg atgcatttgt cgagatcgca ttctatggcg gcagctttac
     1381 ggcaatagac aaggtgcttc aggaagaatt cctgaaacat gcctggagtt atatagaagc
     1441 cgggaaggta tcggagataa gggtatccac gaggcctgac cggatcgata cagaaatatt
     1501 ggaaatgctc aaaaagtatg gagtcaggac catagaactc ggggtgcaga gccttgacga
     1561 cgaggtactc agggccagcc tcaggggcca cgattcggcc gtagttttca gatcggcaaa
     1621 gcttataaaa gatatgggat tcaggcttgg gatacagatc atgacaggat tgcccggcga
     1681 tacaagggaa aaatgcatgg aaacggcacg gaaggtcgtt tcgatgtcgc ctgacgtcgt
     1741 acggatatat ccggttctcg tcattaaagg caccgggctc gaaaggctca tggagaaagg
     1801 ggattacagg ccacagacgc ttgaagaggc cgttgatctg tgcgcagaat tactgaggct
     1861 gtttgaagac aatgggatcg atgtgatcag ggtcggcctg cagcccacgg aaaatataag
     1921 ggaaggcgcc ggctccgatg tggcggccgg tcccgtacac cctgcattca ggcagctggc
     1981 acaggccagg ctttcgttga aaaggatcga agagataata gattcacagc atctctcgga
     2041 tgcacaggaa cttattattt gcaccgggcc ttcgaacatt tccgatgtgg tcggccagaa
     2101 acggtccaat atagaatctt tgaagagcaa atacggtttt gccgatgtca gggtcatgcc
     2161 tgatccgaaa ctttccaggg aaataatatc ccgtgcgatt tcatcggctg attagcactg
     2221 tttacagacc ttgatgtcat ccgttctgtt gtttcgtttt gctgctcaga caaattttcg
     2281 tgtagaactt gttaccaaag ccgtgacgat ggtcatccaa ccatttgggg ttgcttcgtg
     2341 gacagctact ggccggctca tttgctccaa tgagggcgca ggtcactcgc cgtccgtggc
     2401 tcgggaccag cgccggcagc gtccgtgctg ccggttgtgg tgcctgatgc ctcattcgag
     2461 ctgcatgacc cggccgtatc tgtagcactt gcaacccagt aatggtgtgt atgaccatcg
     2521 tcgtgtcttt ggccttgatc ctgaaaatac aaatggcgtc tgcgcagcaa aaccatatat
     2581 aagaacggat gacacaaaac ttaagatgcg ctccaggcag caagctaatg aaataatatt
     2641 gcttaaccca caaattatgt taaaatgaaa atgcaccaat gacaaacgga gggatccatt
     2701 tgtacttaaa gcggctagaa ttacagggct ttaaatcttt tgctgaaaaa atcaggctgg
     2761 atttcaacgg cggcatcacg gcagtagtcg gtccgaacgg cagcggcaaa agcaatatag
     2821 cagatgcgat cagatgggtc cttggagaac aaagcataaa aacactccgc ggaagcaaga
     2881 tggaagacgt tatttttgca ggcaccgagc acaggaaacc tctcggtttt gctgaagtca
     2941 ccatcgtcct cgacaattcc gattcaacgc ttcccataga gtacgacgaa gtcgcgatca
     3001 cacgcagagt ttaccgttca ggggaaagcg aatacctgat caacaaaacc aactgcaggc
     3061 tgaaggatat aacggaactg ctgcttgaca caggtatcgg gaaagacggt tattccgtta
     3121 ttggacaggg aaggatcgac gagatcctca gcacccgctc cgaagaccgc cggcagatat
     3181 tcgaggaagc ctccgggatc atgaaataca aggtcaggaa acaggaagct gagaaaaagc
     3241 ttgaactcac caggcagaac cttgagagga taaacgacat cataacggaa ctcgaggccc
     3301 agttggagcc tctcagggag cagtccgaaa aagcgcgcag gttcctggat ctgagagaag
     3361 aactgaaagt ccttgatgtc aacgtatatc tcgacaacat gaaaaaatcc agggaaaggc
     3421 tcgaaactct ggaacagcag ctaaagacag tacaggacca gataaatgac gaaaataaaa
     3481 aacttgaaga gatcacgaac tcaaacaggg aaaagacaga ccttctcaag tccctggatg
     3541 agaagctcga ggccgcgaaa caggtatatt atgaaaccga aggcaatctt gagcgctgca
     3601 actccgagat atcgctgagc aaggaaaaga tagcaaattt aatgcaaaac attgaaaggc
     3661 tcgacgctga aaaggctgaa ttaaatgaaa agatagaagc ccttgagagc gaggaggcaa
     3721 acaagcaaaa caggctgaat tacctggaga agcagcttga agattacagt accaaactgg
     3781 cggcatatga gaagcagatg gaagaactcc tcaaaacgct ggatgaggaa gaaaggaaaa
     3841 tcgaactgct caagtccgaa ataatggaca agatggacat atattcggat aagaagcttc
     3901 agatcagcaa tctgaggacg catatagaaa atatgcaaaa acgccagaga agcatcgata
     3961 ccgatgtgta ccagaacata gtcgaaaaag acagggaaaa gatgaaaaag gaggaactgg
     4021 ctgaaagcat caggaaagcc gaaaaactca taaaagagca taaggaaaag atatcggccc
     4081 tgtccggcga acgcggggaa accgacagga tgctttcgga actccgtgca aagcagagca
     4141 aaatcaattc agatatacag ttcaaaagct caagggtccg taccctgagg gaaatggagc
     4201 aaaggctgga aggctacaac aggagcgtca gggaagtgct ccaggcctgc catgcgtcgc
     4261 cccagttcgg caaaggcata catggggcat tggcacagct gatcgaggtt gacaggaagt
     4321 acgagacagc catagaaata tcccttggcg gcgctctcca gaacatagtc acacaaaccg
     4381 aagaagatgc aaaaaaggca atcgagttcc tgaaaaagaa caaactagga agggccactt
     4441 tcctgccgat cagttctgta gaaggcaaag gattcgacag ccagacactt gggcgtataa
     4501 agcaatgcga agggttctgc ggcatcgctt ccgacctggt aaagcatgat ccgctataca
     4561 acgggatcat caggagcctg cttggaaaag tagtcgttat agacgatctg gacaacggca
     4621 taaagatggc gcgcaaattt ggctactctt tcaggatagt tactctcgaa ggcgatctgc
     4681 tcaatacggg cggcgcaatt tcgggcggaa gcatcgagaa caggggcaca ggcatcctga
     4741 ccaggaacag ggagataggc gaactggatg aagaactgaa agccctgaag cagcaggagt
     4801 ctgaagtaaa taatgacatc cggctgctca ctgaagatat ttcccggatc gaagccgaaa
     4861 tagcctcgat cgaaaaggaa atcaaggata acgaactcat taagataaga gatgaaagcc
     4921 atcttgcaca gacagacgaa aatatcagca aactgtccgc ccgtatagac atgctcaggc
     4981 aggagaaaga acagcttgag cggcagataa aagaaacaga ggacgaactg gtcaaatatg
     5041 agcttgagct tttgcagacg gaacaggaga tcaacgaagc gaagaaaacc gtatcagagt
     5101 tccaggaaaa gcacaaggaa gaccaggcgg tccgtgatgc tttgcaccag gacataaccg
     5161 atttcaagat ctcggtaaat tctatactgg aaagcatagc aggcgtaaag gagaatgccg
     5221 acaggctccg t
//
LOCUS       DAOSCC010000173         5186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10068_length_5186_cov_4.457026, whole genome shotgun sequence.
ACCESSION   DAOSCC010000173 DAOSCC010000000
VERSION     DAOSCC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10068_length_5186_cov_4.457026"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PLG72_08965"
     CDS             <1..1510
                     /locus_tag="PLG72_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOJ80963.1"
                     /translation="AFLNLFACVAGFEPSVLRAVLMACVLLLAAVIYREPDIYTSIAV
                     SCIIMLVLSPYMLFNIGFQLSYAATLSIVMLYGNISRAIRCRFIPKKTADVIAATLAA
                     QIGVLPVTIMHFNKVSVISLIPNILAAPMLGVITVLGALMAVLGQFSLTLSVLIGYVN
                     NILLSAVLYITKWSASLPWATFRMVTPPAAAVIIYYMMVLYLLWYAPLKGIKLKLKHV
                     SAVLLLSAAVCLMFVVRPACLEVVFLDVGQGDSTFIRTSSGMTVLVDGGGSSNPDVDS
                     GVGELTVVPFLLDSGVASLDAVIATHAHTDHTQGLMDVMEMLGVRNLIIPSLDDEACF
                     AELLELAGNKGIPVTRCSSGMVINLDRNTSLYVLNPEARCKEDEDSLNNTSLVLRLCY
                     KDISLLFMGDAEKEVEEKLANSAFAQLLDADVIKIGHHGSATSTGESFIEAVDPVAAV
                     ISTGRNNFGHPSKKVLRLLEEKGIRYFRTDECGAVLLKSDGKRIRIRGTIGK"
     assembly_gap    1787..1886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2224..3333
                     /gene="holA"
                     /locus_tag="PLG72_08970"
     CDS             2224..3333
                     /gene="holA"
                     /locus_tag="PLG72_08970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ80964.1"
                     /translation="MAPKDSYLGNEKILWKSIKENKIGKLYLFFGPEEYLIRNYCEQM
                     EKRLLEEEFRLLNKVVLNSKATPSQIMENCLTYPVFSNRRLVIVRDSGLFRSGRKSDD
                     TGEAGEDAGKKAKGTRSRGKDADELSEFLQNLPEHICLVFIEKEIDKRVKYVSLAEKY
                     GLVVQFDFRKPDELAEWVIKRLREMQHDASPRTAAMIVDYCEPSMDDILNELNKLCAY
                     AGDRKMINEDDVAKVCTRSVKSRIFDLTDALAAKQSRTALSILNDMAALKEPMPRITF
                     MIARQFRQLIQAKLMVREGAGKNEIASFFKVPPFIAEKIIKQAAGFDMDSLRKAVDTG
                     LELDLAVKNGRLDDKAAIELLITSLLAPGRSISAR"
     gene            complement(3459..3725)
                     /gene="rpsT"
                     /locus_tag="PLG72_08975"
     CDS             complement(3459..3725)
                     /gene="rpsT"
                     /locus_tag="PLG72_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ80965.1"
                     /translation="MANTKSAAKRVLVIRKKTIRNSMKKSALRTAVRKCREAIAANSE
                     SAPEILKATIKAIDKAAAKNILHKNTAARKKSQLVRAFNAAAGK"
     gene            3879..4880
                     /gene="gpr"
                     /locus_tag="PLG72_08980"
     CDS             3879..4880
                     /gene="gpr"
                     /locus_tag="PLG72_08980"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOJ80966.1"
                     /translation="MAELGNKRTDLTLEAHELLREKAVREQKAQQDIPGIEVENDGDE
                     RIKITRVRVTSRTGEVSIGKPMGNYITLEVPGLKENDRDLYEDTCRALAKELTSIMKL
                     EDKTLTLVIGLGNWNVTPDALGPKVVSNMMVTRHLLEYLPDQVDEGVRPVCAVAPGVL
                     GITGIETGEIVKGIVDRIHPDYIIAIDALAARKLERVNTTIQIADTGIAPGSGVGNKR
                     MELSKDTLGVPVLAIGVPTVLDAATMANDAIDLVLDSLIGQARQGTDFYNMLKNIDRE
                     EKYSLIREVLEPYSGNLIVTPKEIDDVVRRVSKVIANGLNIALHKGIDLDDIDRYIN"
BASE COUNT         1348 a         1169 c         1391 g         1178 t
ORIGIN      
        1 agcgttcctc aatctgtttg catgtgtcgc cgggttcgaa ccgtctgtgc tcagggcagt
       61 gttaatggcc tgcgttctcc ttctggccgc tgttatttac cgggagccgg acatctatac
      121 ttcgatcgct gtttcatgca tcattatgct ggtgttaagc ccgtacatgc ttttcaatat
      181 cggtttccag ctctcgtatg ctgcaacact gagcattgtg atgctgtacg ggaatatcag
      241 ccgggcaata cgctgccgtt tcatcccgaa aaagactgcc gatgtgatcg cagcgaccct
      301 ggctgcccag atcggtgtgc tccccgtaac gatcatgcat ttcaataagg tctctgtcat
      361 ttcgctgata ccaaacatac tggcagcgcc gatgctcggc gtaataaccg ttctcggggc
      421 gctgatggcg gtcctggggc agttcagcct cacactgtcg gtacttatcg gatatgtgaa
      481 caatattctg ctgagtgctg tgctgtacat caccaaatgg tctgcttcgc tgccatgggc
      541 gactttcagg atggtcactc cgccggcggc tgctgtcatt atctattaca tgatggtatt
      601 atacctgctc tggtatgccc ctttaaaggg aatcaagctg aaactgaagc atgtatccgc
      661 agtattgctg ctttcggcgg ctgtttgcct tatgtttgtc gtcaggcccg cctgccttga
      721 ggtggtgttc cttgatgttg gccaggggga cagtacgttc atcaggacaa gcagcggcat
      781 gacagtgctg gtcgacggcg ggggcagcag taatccggat gtggattcgg gagtcggaga
      841 actgacagtg gtgccgtttc tgctggacag tggagttgca agccttgacg ccgtcatagc
      901 cacccatgcc cataccgacc acacacaggg gcttatggat gtcatggaga tgcttggagt
      961 cagaaatctc atcattcctt cactcgatga tgaggcctgt tttgcggaac tgctggaact
     1021 ggcggggaat aagggcatac ctgtaactag atgttcgagc ggcatggtga taaacctgga
     1081 caggaacacc tccttgtatg tgctaaatcc ggaagcccgc tgcaaagaag atgaggattc
     1141 gctcaacaac acgtcgcttg tgctgaggct gtgctataaa gacatttcgc ttctctttat
     1201 gggggacgca gagaaagaag tcgaggaaaa gttggcgaac tcggcgtttg cacagttgct
     1261 cgacgctgat gtcatcaaaa tagggcacca tggttccgcc acttcgaccg gcgaatcatt
     1321 catcgaagcg gtcgatcccg tggcagcggt gatcagcacc ggcaggaaca atttcgggca
     1381 cccttcgaaa aaagtgctca gactgcttga agagaagggg ataaggtatt tccggacgga
     1441 tgaatgcggg gccgtgctgc tgaaaagcga cgggaaaagg atacgaatca ggggcactat
     1501 tggcaaataa gcataatgca tgctgtgccg taacgtgcaa atatgtttgt gatgctgtgc
     1561 aatgcaagta aacataagcg aagcgcagcc tagagatgta tagtcagagc gaggaccagc
     1621 aagccgactg tctgagcaaa aatgccacga cgaaaaatcc attccatgca tttttgcgag
     1681 tttcggcttg cccgagctga actataacag ctcttgcaaa cggagcttca gtttacggca
     1741 ttgcacagca tcacaggcat accgtgatac tatggaggta ctatgcnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnttta cggcattgca cagcatcaca ggcataccgt
     1921 gatactatgg aggtactatg ctgcgatact gtgcaatgca agtgaacata agcgtagcgc
     1981 agcttagaga tgtatagtca gagcgaggac cagcaagccg actgtctgag caaaaatgcc
     2041 acgacgaaaa atccattcca tgcatttttg cgagtttcgg cttgcccgag ctgaactata
     2101 acagctcttg caaacggagc ttcagtttac ggcattgcac agtatctcag gcatgtccat
     2161 gccttgaaaa agggccggcg tattgctaaa atcaagaaaa gcacataaag agagttggat
     2221 tgtatggcgc caaaagactc gtatctcgga aacgaaaaga tactctggaa atccataaaa
     2281 gaaaacaaga taggaaaact ttaccttttc ttcggacccg aagaatatct cataaggaac
     2341 tactgcgaac agatggagaa aagactgctg gaggaggaat tcaggctgct gaacaaggtt
     2401 gtcctgaata gcaaggcaac gccgtcgcag ataatggaga actgccttac atatcctgtc
     2461 ttcagcaaca gaaggctggt catcgtcagg gattcgggcc ttttcaggtc cggcagaaaa
     2521 agtgacgata cgggcgaagc aggcgaagat gcagggaaaa aggcaaaagg gaccagaagc
     2581 aggggaaagg acgctgatga gctttcggag tttctccaga atttgcccga acatatctgc
     2641 cttgttttta ttgagaaaga gatcgacaaa agggttaaat atgtcagcct ggcggaaaaa
     2701 tacgggctcg tggtgcagtt cgatttcagg aaacccgatg aactggcgga atgggtaatc
     2761 aagaggctga gagagatgca gcatgacgcg tcgccgagaa cggctgcgat gattgtcgac
     2821 tactgtgagc cgtcaatgga tgatatactc aatgaactca acaagctgtg cgcatatgcc
     2881 ggtgaccgga agatgataaa tgaagatgat gtcgccaaag tctgtaccag gtcggtcaaa
     2941 agcaggatct tcgacctcac cgatgccctg gcggccaaac agagccgtac cgcgctttcg
     3001 atcctcaatg acatggcagc gctgaaagag cctatgccaa ggatcacatt catgatagcc
     3061 aggcagttca ggcagttgat ccaagccaag ctgatggtgc gggagggcgc agggaagaat
     3121 gaaatagctt cgttcttcaa agtgccgcca ttcatagccg agaaaataat aaagcaggct
     3181 gccggctttg atatggacag cctcaggaag gcagttgata ccggacttga actggatctt
     3241 gccgtgaaga acggcaggct ggacgacaag gcggccatag agcttctgat cacaagcctt
     3301 ttggcgcccg gcaggtccat ttccgcccgg taaagcaaaa ctgccggaac aggcagcccg
     3361 gcaaaataaa aaagcccggc cgcctggaat tttccatttg ctgctcgcgc tttttttatt
     3421 gtgctattta tggatacagc aaatcccgga atcgggaatt acttgcctgc tgcagcattg
     3481 aatgccctta ccagctgtga tttttttctc gcagcggtgt tcttgtggag gatgttcttt
     3541 gctgctgcct tgtctatagc cttgatcgtg gctttcagga tctcaggcgc agactcgctg
     3601 tttgcagcta tagcttccct gcacttcctg acggcagtcc tgagagccga cttcttcatg
     3661 ctgttcctga tggttttttt cctgatcacc aatacccttt tagcagcaga ttttgtattt
     3721 gccaagcttt tcacctcctg cggtccgatt tttttcaaca tgtgatattc taccatgaac
     3781 atttttaaaa agcaaggata aaaaaaacgg gttttgtgaa aaactaaaac ccggtactat
     3841 gcataaatcc ggaatcatat caaggagaga aaccaacgat ggcggaactg ggaaacaaaa
     3901 ggacggacct tactctcgaa gcccatgagc ttttaaggga gaaggcggta agagagcaga
     3961 aagcgcagca ggatatcccc ggcattgaag ttgagaatga cggggatgag cggataaaga
     4021 taaccagggt ccgggtcacg tcccgtaccg gcgaggtttc catagggaag ccgatgggca
     4081 actatatcac tcttgaggtg ccgggactga aggaaaatga cagggatctg tatgaagata
     4141 catgcagggc cctggcaaaa gagctgacaa gcataatgaa gcttgaagac aaaacgctga
     4201 ctctggtgat cggcctggga aactggaacg ttactcccga tgcccttggc ccgaaagtgg
     4261 tgtcgaacat gatggtgacc cggcatctgc tggaatacct gccggaccag gtcgatgaag
     4321 gcgtaaggcc ggtctgcgcc gtagctcccg gtgtcctcgg cattacgggg atagagacgg
     4381 gtgagatcgt aaagggaata gtcgacagga tacaccctga ttatatcata gccattgatg
     4441 cacttgcggc aaggaaactg gaaagagtga acacgacgat acagatagcg gataccggta
     4501 ttgccccggg ttccggtgta ggcaacaaaa ggatggaact ttcaaaagac acgctgggcg
     4561 tacctgtact ggctataggc gtaccgacgg tgctggacgc ggctaccatg gcgaacgatg
     4621 ccatcgacct ggtgctggac agccttatcg gacaggcccg gcagggaaca gatttctaca
     4681 atatgctgaa gaacattgac agggaagaga aatacagcct cattcgcgaa gtgctcgaac
     4741 cctattcggg aaacctgatc gtcacgccca aggagatcga cgatgtggtg agaagggttt
     4801 cgaaagtcat agccaatggg cttaacatag ccctgcacaa aggaatcgac cttgatgaca
     4861 tcgaccggta tataaattaa gcaaaggaaa gcagcgtcgg cgcgtcggct ggcggcagct
     4921 gccggtaccg ctcattccgg gctttatttt gacccccgaa agaagtataa ttaagaaagc
     4981 gcagttatat ttacaatgta tccactattc gtcattcggg ggtcgggatg aagcaaagcg
     5041 gaagatatgt ggtcatactc atagcggcaa tggcattgtg gaataccgtt gtaataaagc
     5101 cgttgaaaat actgtccgtt tttttccatg aactgggcca ttcattcatg tccgtcattt
     5161 tcgggaatgg tatagcaggt ttcagg
//
LOCUS       DAOSCC010000174         5184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10071_length_5184_cov_3.351725, whole genome shotgun sequence.
ACCESSION   DAOSCC010000174 DAOSCC010000000
VERSION     DAOSCC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10071_length_5184_cov_3.351725"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLG72_08985"
     CDS             complement(<1..710)
                     /locus_tag="PLG72_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ80967.1"
                     /translation="MYRFVGFKWFQTMVSNKVRVTQLLSVLRNTFAISGISLAFSWFP
                     MMFAVFLNEIRCTPFKKFVQTVTTLPNFISWVLVYSIAYSVFSSTGAVNTIAMNLGLL
                     DEPKIFLASSEHIYFKMWLWLTWKNAGWAAIMYIAAIAGIDEELITAAKVDGASRMKI
                     IWYVTIPSLLPTYFVIVMLNLANFLSNGFEQFYVFQNAFNKEYIQVLDLYVYTLAMGN
                     GGYSLSVAVSMFKSIVSIV"
     gene            840..974
                     /locus_tag="PLG72_08990"
                     /pseudo
     CDS             840..974
                     /locus_tag="PLG72_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440545.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
     gene            complement(1227..>1635)
                     /locus_tag="PLG72_08995"
     CDS             complement(1227..>1635)
                     /locus_tag="PLG72_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOJ80968.1"
                     /translation="GFLRSETSLIQTIGRAARNVEGKVIMYADTITDSMQRAISETNR
                     RRKIQMEYNLKHGITPTTVQKGVRDTLETIKAAEGGTKYAFTQDTEQMDESDRQALIG
                     KLEKEMKEAARELQFEKAAFLRDRLMELKEKMK"
     assembly_gap    1636..1645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1646..3134)
                     /gene="uvrB"
                     /locus_tag="PLG72_09000"
     CDS             complement(<1646..3134)
                     /gene="uvrB"
                     /locus_tag="PLG72_09000"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOJ80969.1"
                     /translation="MPEFKIVSEYKMCGDQPEAVDRLVKGLNRGYRHQTLLGVTGSGK
                     TFTMANVIERVQRPTLVIAHNKTLAAQLCSEFREFFPNNAVEYFVSYYDYYQPEAYIP
                     STDTYIEKDSSINDEIDKLRHSATAALFERRDVIIVASVSCIYGLGDPEDYTDLMISL
                     RPGMIKDRDEVIRKLVDIQYERNEVDFRRGRFRVRGDVLEIFPPGSTDHVLRVEFFGD
                     EIEKISEVDYLTGEIIGVRTHAAIFPANHYATTREKMLKAVSSIEQELEERLKEFREQ
                     GKLLEAQRLEQRTRYDLEMLQEVGFCQGIENYSRHISGREPGSPPYTLLDYFPDDMLV
                     FIDESHVTVPQIGSMYNGDRSRKESLVEYGFRLPSAFDNRPLTFEEFEGKVKQAIYVS
                     ATPGKYEREHSQQIVEQIIRPTGLVDPEVEVRPVKGQIDDLIGEIRIRVEKQQRVLVT
                     TLTKKMAEDLTAYLKELGFKVEYLHSDVETLERVELLRDLRLGVFD"
     gene            3559..4908
                     /locus_tag="PLG72_09005"
     CDS             3559..4908
                     /locus_tag="PLG72_09005"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ80970.1"
                     /translation="MIVAKFGGTSLASASQIKKVCNIITSDPERRIIVVSAPGKRSRD
                     DIKVTDMLIACAQAGLEKGNADVEMNAVVARFAEIASELGLSDDIIDTIAGDIRARVN
                     SDKSNKEKYTDNLKAAGEDNSAKLVASYLRSIGYDARYVNPKDAGLILSDEYGNAQVL
                     PQSYENLKTLSDYPGITIFPGFFGYSLSGEVVTFPRGGSDITGSILAAAVDADLYENF
                     TDVDNVYSANPNLIENPKPIIEITYREMRELSYAGFSVLQEETLIPVYKKGIPVRIKN
                     TNNPSAPGTTIVKERHNITEPVVGIAGDSGFCFIYLSKYLMNREIGFGRKLLQILEEE
                     HIPFEHMPSGIDNITIILKQAHFTEQKEKKIVDRIVNELEVDDISVQRNLALVMIVGE
                     GMRNTVGITSRASGALARENINLDLISQGPSEVSMIFGIKDYEKDRAIRALYNEFFK"
BASE COUNT         1249 a         1308 c         1206 g         1411 t
ORIGIN      
        1 acgatactta cgatgctctt gaacatactg accgcgacag aaagcgagta acctccgttg
       61 cccattgcaa gggtgtatac gtacaggtcg agaacctgga tatattcctt gttgaatgca
      121 ttctggaaca cgtagaactg ctcaaatccg ttggagagga aatttgccag gttcagcatt
      181 acaattacga aataggtcgg cagcaggctt gggatagtta cgtaccaaat gatcttcatc
      241 ctggaagcgc cgtccacttt agccgcggtg atcaattctt cgtcgatgcc ggctatggcc
      301 gcaatgtaca tgatagccgc ccaaccggca ttcttccagg tcagccagag ccacatcttg
      361 aaatatatgt gctcggatga tgcaaggaat atcttcggct catcaagcag tccgaggttc
      421 atcgctatcg tattgacagc gcccgtgctg ctgaataccg aatatgctat cgaatataca
      481 aggacccagc ttataaaatt ggggagtgtt gtgactgtct gaacgaactt tttgaatgga
      541 gtacacctga tctcattgag gaatactgca aacatcatcg ggaaccatga gaaagcaagg
      601 cttatcccgc ttatggcaaa tgtgttccgc aaaaccgaga gaagttgtgt gaccctgact
      661 ttgttcgaaa ccatcgtctg gaaccacttg aagccgacaa acctgtacat cctgtgatcc
      721 cgatccgttg ctgactgcat ggcttaatgc atgtcaacag tatataagta ccatacctcc
      781 caggatactt atgaatatac ctgcacctgt ccgatcccgt gccggcaaca cttgcaacca
      841 aagtgcaaat aaccgcagat agcgcttagc ctaagtctat tgctggattg caaggacttg
      901 gtttacgcca tattcgaggc gctaaaaagg gtaaagacag tattgccaat ggtattcagt
      961 tcatacagaa ctttataccg tcggatgctg gaatgatttt tccgattata atattgcatg
     1021 tgcttggttt attatccggt ttgatctttg attgccgaat caatcatacg gtcatgccgg
     1081 acctttcaaa gtcgatctga tatttacagg ccatttgggc tcttccggat cattcggaca
     1141 gctcatttgc catttgatta tcttcagatt atcggtccgg tccatagcaa tattttcaag
     1201 ttatcaaatc tgtccataac atcacgtcac ttcattttct ccttcagttc catcaacctg
     1261 tctctcagga atgcggcctt ttcaaactga agttctctcg ccgcttcctt catctctttc
     1321 tccagcttgc cgatcagtgc ctgccggtca gattcgtcca tttgctctgt gtcctgagta
     1381 aatgcgtact ttgtaccacc ctctgcggct tttatcgtct ctagtgtgtc acgcactccc
     1441 ttttgaacgg tagtcggagt gatcccatgc ttgaggttat attccatctg tatctttctt
     1501 ctcctgttgg tttcgctgat ggctctctgc atggaatccg tgatggtatc ggcatacatt
     1561 atgactttcc cctcaacatt cctcgccgca cggcctatgg tctgtatcag cgatgtctcg
     1621 gaacgcagga atcctnnnnn nnnnncgtcg aaaacgccga gtctgaggtc tctcaaaagt
     1681 tcgactcttt caagggtttc aacatcggaa tgcaggtact ctactttgaa gccaagttcc
     1741 ttcagatagg ccgtcaggtc ctccgccatc tttttggtca gcgtggtgac caggactctt
     1801 tgctgttttt cgacgcggat cctgatctcg ccgatcagat cgtctatctg gccttttacc
     1861 ggacgcactt cgacttccgg atcgaccagt cccgtaggtc ttattatctg ctccacgatc
     1921 tgctgcgagt gttctctttc atactttccg ggtgtggcgc tgacatatat ggcctgtttg
     1981 actttccctt cgaactcttc gaacgtcagc ggcctgttgt cgaacgccga cgggagcctg
     2041 aagccgtact ccaccagcga ttccttcctt gaacggtctc cgttgtacat gcttccgatc
     2101 tgagggacgg tcacgtggga ctcgtctatg aagaccagca tgtcgtccgg gaagtaatcc
     2161 agcagtgtat atggcggact gccgggctcc ctgccgctga tatgccttga gtagttctct
     2221 atgccctggc agaaaccgac ttcctgcagc atttcaagat cgtacctggt cctttgctca
     2281 agccgctgcg cttccagcag tttgccctgc tccctgaact ccttcagcct ctcctccagt
     2341 tcctgctcta tcgatgatac ggctttgagc atcttttccc tcgtcgtcgc gtaatggttc
     2401 gcagggaaaa ttgcggcatg cgtccggaca cctatgatct cgccggtcag gtaatcgact
     2461 tccgatatct tctcgatctc gtctccgaag aactccaccc tcaggacatg gtccgtcgag
     2521 cccgggggga atatctcaag cacgtctcct cttaccctga acctgcctct cctgaagtcg
     2581 acctcattcc tttcgtactg gatatccacc agtttcctga tcacttcatc cctgtccttt
     2641 atcatgccgg ggcgcagtga gatcatgaga tccgtatagt cctcggggtc gccgaggccg
     2701 tagatgcagg atacgcttgc cacgatgatg acgtcgcgcc tctcaaaaag cgctgccgta
     2761 gcagagtgcc tgagcttgtc tatctcatcg tttatagatg aatccttttc tatatatgtg
     2821 tccgttgacg gtatatatgc ttcaggctgg taatagtcgt agtagctgac gaagtactcg
     2881 accgcattgt tggggaaaaa ctccctgaac tcgctgcaca gctgggcagc cagcgtcttg
     2941 ttgtgcgcta tgaccagcgt cggcctctgg accctttcaa taacgttcgc catcgtgaac
     3001 gtcttcccgg atcctgtcac gccaagcagc gtctggtgcc tgtagcccct gttcagcccc
     3061 ttcaccagcc tgtccaccgc ctccggctga tcgccgcaca tcttatattc cgatactatc
     3121 ttaaactccg gcatacatcc tccttttagt ggggacactt ctcacctttt tgacattttc
     3181 agagcaatcc gcctggaggg acgcttctca cttttgtgac attccctcat ccggatcaac
     3241 tgatacctta cctgtccctc cggtagggga cagttctcac ctttttatct tttcaaagag
     3301 gcactgaaaa catactggag catcatcgtc ctgctatgtt cagtccggca cggctgtgat
     3361 gctgttcgcc gaaccgttaa tcacgtattt taacatttat aaaagtgttg aaaacgtgag
     3421 aactgtcccc accattatag cataaattat aagtgaaatt tttcaatttt ccatgtaact
     3481 tgtgacgatt ttttgttgac atgacctttt gtctggtgtg ataatatgag gaaaactact
     3541 atagtgggag ctggtagtat gatcgttgca aaatttggcg gaacttctct ggcgtctgcc
     3601 agtcagataa agaaagtatg caatatcatc acctctgacc cggaacgcag gataatcgtg
     3661 gtttctgccc cgggcaagcg cagcagggac gatatcaaag tgacggatat gctgattgcg
     3721 tgcgcgcagg caggtctcga aaaaggtaat gccgacgttg aaatgaacgc agtggtggcg
     3781 agatttgcag agatcgcgtc agaacttggt ctttccgatg acattatcga taccatcgcc
     3841 ggcgacataa gggcacgagt gaacagtgac aaatcaaaca aggaaaagta cacagataat
     3901 ctcaaggcgg caggcgaaga caacagtgcg aagcttgttg cctcatatct gcggagcatt
     3961 ggttatgatg cccgttatgt caacccgaag gatgccggcc taatactcag cgacgaatac
     4021 ggcaatgccc aggttctgcc tcagtcatac gagaacctga aaacgctttc ggattatccg
     4081 gggatcacca tattccccgg ctttttcgga tattcgctga gcggcgaggt cgtaacgttc
     4141 ccgagaggcg gctccgatat tacaggttcg atacttgccg ctgcagtaga tgccgatctt
     4201 tatgagaatt ttactgatgt ggacaatgtt tacagcgcca atcccaatct gatagaaaac
     4261 ccgaaaccga taatcgagat tacatatcgc gaaatgcgcg agctttctta tgcaggtttc
     4321 agcgttctgc aggaagaaac tctgattccg gtatacaaaa agggcattcc tgtaaggata
     4381 aaaaacacaa acaatccgtc agctcccgga accaccattg taaaggaacg ccacaatata
     4441 acggagcctg ttgtaggtat cgcaggcgat tcgggatttt gctttattta cctgagcaaa
     4501 tacctgatga accgcgaaat cggttttggc cgcaaactgc tgcagatcct ggaagaagag
     4561 catattccgt tcgagcacat gccgtcaggg atcgataata tcacgataat actgaagcag
     4621 gcccatttca ccgaacagaa agaaaagaag atcgtcgacc gtatcgtaaa cgagcttgaa
     4681 gtcgatgata tttcggtgca gaggaacctt gcccttgtca tgatcgtcgg cgaaggcatg
     4741 cggaatactg tcggaatcac ctcaagggcc tctggtgcgc tggcaagaga gaatatcaat
     4801 ctggatttga tcagccaggg tccatccgag gtaagcatga tattcggaat aaaagattac
     4861 gagaaagaca gggctataag agctctgtac aatgagttct tcaaatgagt tgtgattcga
     4921 gtttcgcaga tatgcgggga ttcagtttgt tatttgatga gtttttcagc agctacctga
     4981 gaatacagat gctggagaac aattatgtaa gtgcttcagc gtatagaata atgacataat
     5041 aataacttag gtgctgcggt tttcgtccag gcttttagtt atgctatatc aggtcaaggc
     5101 gccatagcag ttcaagaatt gctgactgtt ccggcagcca ggctgaaatg gtcaaacgcg
     5161 gccaattcct gccggaacaa tgcc
//
LOCUS       DAOSCC010000175         5174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10091_length_5174_cov_4.360031, whole genome shotgun sequence.
ACCESSION   DAOSCC010000175 DAOSCC010000000
VERSION     DAOSCC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_5174_cov_4.360031"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..4177
                     /locus_tag="PLG72_09010"
     CDS             197..4177
                     /locus_tag="PLG72_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ80971.1"
                     /translation="MKQNLRENSRINNGIYRRPHRSAAGILAFLLLAAQFIIAVPAAA
                     ADNPPQPAPEKLRVEAIYDQAPNVQPAIGYNEYDKYYVDLKSDKIARPLNTPATPSIY
                     LNYYLQEVTKPYKPAKPTILKEADVPADTSADNKIRLRELNSGTVYYAYSRAYYTYTT
                     AEAGTYTSPESTPSNTVKFLTDIEIEAYSYGPNQIRIVWDDVWNAGKRMDYKLYVSEN
                     SQFTNTPPIYIGQEQIGQNGPVRVNESTGKLEYIHTVRDPGRVYYIKIEPNTTETELK
                     RSPSSKVVAVSSFILAKTTRVSVDPATGNTIWKLEWSPVVTGLSGSDIKITYHIYRGT
                     GTAGSIEQYIAAKNDTTFFLTVAPGEENYYYIIKAIVTRNGQDLYPGIPITSSKIYIK
                     ESEVAAKPAVPELVTELSDAAMTVRQEINATDATILWRVPRKGNGEVDSDVMYDIWLV
                     TDPNLINNPPAGSKIASDLKMNQGNYVMSGNILLGYKYTIRDLVPNTTYYLKIVAKKN
                     YIEYVDSMLQNVTLESDPALKMFITPALGPTDQPVVPGTPPLKLKKDKQGKEMVTSTT
                     VVITLKNKWYEEYTNKKTPDAEPGEYSWYFRTPSQLDEISADLVDKLESGDSSVNPLR
                     FRKVEYDEGVTIDVGVIEYVPGMDYNDLENIPADKVIGHTVTANDPDEDINAEDNVRD
                     NLRHNVDITLYDLEPNKTYVIWVRAVRRSVNLMSGPSDPILVTTMPELPVEAEKPTVP
                     VFNYHYAGDTFIDLGWNFNSQYIYYLVYGTADDMSKAVGRITITPQDLEYTTYYRVNG
                     LKPDTVYYFWIQAEAASAAGEKKQSDFSDSYIVKTQKEIPPDTPLGFGVKDRTKDSIT
                     YEWLAVEGMEYILEIDDGIDYAGSTRYHITNASEFTVTGLRSNFRYYARLYAYDPAKQ
                     LESAPTQSVVVRTLRSTDDYDSSEDPDYVIIGDFIIKDSYAVNGIWKVRITGVNADRF
                     IQYVKTDNVLDYVIDLKTMPQGTKTISLIISQKVFKALGTLGENLMLRTVSNTIIIRP
                     GVMADKNGIYGSATSESDFVIDIVLQAAAAGDTGNMTFKTPISDIAVSLSDGLPKKIS
                     SFGKPLKVEYEYTSDGWYIPGTTYGYYLPSGESSWKKVSLQRNYNYDAGIGTLAFEVP
                     VPGRMAIADQGSDFYTDISRSYARRSIANVASVHELKSVTGKKFEPEKDLTVGAAVKF
                     MLDIMDTDYGVNYMTLAARAGIINAADTGRADEKCTREQLISMAARVCEIKTGDRIGN
                     DQTDLSVYKDIGQASPALLPRIRYAYRTGVITSRFSDTLGPKDIVTRAEAMVLLEKLL
                     RYAGEL"
     gene            4690..>5174
                     /locus_tag="PLG72_09015"
     CDS             4690..>5174
                     /locus_tag="PLG72_09015"
                     /inference="COORDINATES: protein motif:HMM:NF020264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HOJ80972.1"
                     /translation="MKAFEMTGAEATASEIYIRGRSENPGFNEEMSLSLLVEIVSGIG
                     SGTGGAVPVFCPIDTDFAIGHEVNYIIDENKKIHMTVLDQKGPETGNTLLISLYDISS
                     EPELQKCYEAVTEVLDKYSIDHEINITVTGFVKGKLDDDETADIFDRAMGSTGASRVE
                     G"
BASE COUNT         1506 a         1250 c         1337 g         1081 t
ORIGIN      
        1 gatcaatact atatgtccgt tctgatagtc gtcgacatca atgtaatgca gcttgacgcc
       61 tcaggaaggt tccggcccag gggcaccatg acccgtgccg aggctgtggc cgcattcgca
      121 aggctgctgg aactgaccgg cgaactgtaa actgggtggt cggcagccga tgtatcgagc
      181 aagaggtgat atgccaatga agcaaaactt aagagaaaac agcagaataa acaacgggat
      241 atacaggaga ccgcacagga gtgcagcagg catactggcc tttttgctgc tggccgcgca
      301 gttcatcatt gcagttccgg cagctgcggc agacaatccg cctcagcctg cgcccgaaaa
      361 gctcagggtc gaagccattt acgaccaggc gccgaatgtt cagcctgcca taggctataa
      421 cgaatacgac aaatattatg tcgacctgaa gagtgacaaa atcgcgcgtc ccctgaatac
      481 tccggctacc ccgagcatat acctgaacta ctatctgcag gaagtaacca agccgtacaa
      541 gccggcaaag cctactatcc tgaaggaagc ggatgtgcct gcggatactt ccgcagacaa
      601 caagatacgc cttagagaac tgaattcagg tactgtttac tatgcttatt ccagggccta
      661 ctacacttat actactgccg aggcgggcac ttataccagc cctgaatcga caccgtccaa
      721 tacagtaaag ttccttacgg atatagagat agaagcctat tcctacggcc cgaaccagat
      781 aagaatagtg tgggatgacg tgtggaacgc aggcaaaaga atggattaca aactctacgt
      841 ttctgaaaac agccaattta caaatactcc tcccatatat atcgggcagg agcagatagg
      901 ccagaacggg ccggtgagag tcaacgaatc gaccggcaag ctcgagtaca tacacaccgt
      961 aagggatccg ggaagagtat attacataaa aattgagccg aatacaaccg aaacggaact
     1021 caaacggtcg ccttccagca aggtcgtagc tgtcagcagc ttcatacttg ccaaaacgac
     1081 cagggtgtcg gtggatccgg ccacaggcaa caccatatgg aaactggaat ggagccccgt
     1141 ggtcacgggc ctgagcggca gcgatataaa aatcacgtac catatataca ggggcaccgg
     1201 gaccgccggc agcatagagc agtatatagc ggcaaagaat gacactacgt tcttcctgac
     1261 ggtagcaccg ggcgaagaaa actactatta catcataaag gcaatagtta cgaggaatgg
     1321 ccaggatctt tatccgggca taccgatcac ttcttcgaag atatatatca aggagtcgga
     1381 agtcgctgcg aaaccggccg taccggaact agtcaccgaa ctgtccgacg ctgccatgac
     1441 ggtaaggcag gagataaacg ccaccgacgc gactatcctg tggagagtcc cgcgcaaggg
     1501 gaacggggag gtcgacagcg atgtcatgta tgatatctgg ctcgtaaccg acccgaatct
     1561 tataaataat ccgccggccg gcagcaaaat agcatctgac ctgaaaatga accagggcaa
     1621 ctatgttatg agcggcaaca tactgcttgg ctataaatat acgatcaggg acctggtccc
     1681 gaacaccacg tattatctga agatcgtagc aaaaaagaac tacatcgaat atgtggacag
     1741 catgctgcag aacgtgacac ttgagtctga tccggcgttg aagatgttca taacgccggc
     1801 cctgggtccc accgaccagc ctgtcgtgcc cggaacgcct ccccttaagc tgaaaaagga
     1861 caaacagggc aaggaaatgg tcaccagcac gacggttgtc ataacgctga agaacaaatg
     1921 gtatgaggag tacacgaaca aaaagacacc ggacgccgaa ccaggcgaat attcctggta
     1981 tttcaggacg ccttcgcagt tggacgagat aagcgctgat ctggtcgaca agctggagtc
     2041 cggcgatagt tcagtcaacc cgcttagatt cagaaaagtg gaatatgacg agggagtgac
     2101 cattgacgtc ggtgttatcg aatatgtccc gggaatggat tacaatgacc ttgaaaatat
     2161 accggctgac aaggtgatcg ggcacacggt gacggcaaac gatcccgacg aggatatcaa
     2221 tgctgaagac aacgtcaggg ataacctcag gcacaatgtg gacatcacgc tgtatgacct
     2281 cgaaccgaac aagacctatg taatatgggt gcgggctgtc cgccgcagtg taaacctgat
     2341 gtcaggcccg tcggatccca tcctggtgac gacgatgccc gaactgccgg tcgaggcaga
     2401 gaaaccgact gtacctgtgt tcaattatca ttatgccggc gacacattca tagatcttgg
     2461 ctggaacttc aacagccagt acatttatta cctggtgtac gggacagccg acgacatgtc
     2521 caaagccgtc ggcaggatca cgataacgcc gcaggatctg gaatatacca catattacag
     2581 ggtaaacggc cttaagccgg atacggtcta ttatttctgg atacaggcgg aagcggccag
     2641 cgccgcaggc gagaaaaaac agtctgattt cagcgactcc tatatcgtga aaacgcaaaa
     2701 ggagatacct ccggatacgc ctctgggatt cggcgtaaag gacaggacca aagacagcat
     2761 aacatatgaa tggctggctg tggagggaat ggaatacata ctcgaaatag atgacggtat
     2821 cgattatgca ggcagcacaa ggtatcatat caccaatgcg tccgagttca ctgtcaccgg
     2881 cctgagatcc aacttcaggt attatgcaag actgtatgca tatgatcccg caaaacagct
     2941 ggaatcggcg cctactcaaa gcgtcgtggt ccggacgctc cggtcgactg atgattacga
     3001 ttccagtgaa gaccccgact atgtgataat cggcgatttc atcataaagg acagctatgc
     3061 ggtaaacggc atatggaagg tccggatcac gggagtcaac gctgaccgct tcatacagta
     3121 tgtaaaaacc gataacgtcc tcgattatgt catcgacctg aaaaccatgc cccaggggac
     3181 aaaaacgatt tcgctgataa tctcgcaaaa ggtgttcaag gcattgggca cacttggcga
     3241 aaacctgatg ctcaggactg tcagcaatac cattattatc cgcccaggag tcatggctga
     3301 caaaaacggc atatatggtt cggcaacttc cgaatccgat ttcgttatag atatcgtcct
     3361 gcaggctgct gctgcaggcg ataccggcaa catgacattc aaaaccccta tatcggacat
     3421 cgccgtgagc ctgtccgacg gtttgccgaa aaagatcagc agcttcggca aaccgctgaa
     3481 ggtggaatat gaatatactt ccgatggatg gtacatacct ggcaccacgt atggctacta
     3541 tctgccgtcg ggtgagagct cctggaaaaa ggtatcgctg caaagaaact ataattatga
     3601 cgccggtatc ggtacattgg catttgaagt gccggtcccc ggcaggatgg cgatagccga
     3661 ccagggatcg gacttctata ccgacatatc aaggagctat gcaagacgtt ccatagccaa
     3721 tgtcgcatcg gtccatgaac tcaaatcagt gacagggaag aagttcgagc ctgaaaaaga
     3781 ccttactgtc ggtgcggcgg tcaagttcat gctggacatc atggataccg actacggcgt
     3841 caactatatg accctggccg caagggcagg tataataaat gcggcggaca caggcagggc
     3901 cgatgagaaa tgtacgagag aacagctcat atcgatggcg gcaagggtat gcgagataaa
     3961 aaccggggac aggatcggga atgaccagac agacctgtcg gtatataagg acattgggca
     4021 ggcaagcccg gcgctgctgc cgcgtataag gtatgcatac cgtaccggcg tgataaccag
     4081 caggttttca gacacgctcg gcccgaagga tatcgtcacg cgggcagaag cgatggtgct
     4141 gctggagaaa ctgctgcgct acgcgggtga actgtaaggc tgtacgccgg aaatctcaaa
     4201 gaatgcctgc atgttttgcc gcaggacaga aaaaggcaac tgataaaggc atgattatta
     4261 cggaaaacaa cgtatttttc tgcttaattt ttcataaagc atattggaaa gccatatcaa
     4321 agcatcgcga ggcggcatca cacttttgcc gcctcgcatt ttgcatctca aagtgctcat
     4381 atcctgactt tattaaattt gaagaattat gatcaccatc cagcaatgac gtcgggcata
     4441 gaattttttc atgtatacgc cctatcagat gaggcaataa ttctagtgga accgaaccgc
     4501 aatgattatg tcaagctggt tttgcggcat tgacccgtta tccggccggg catggcaaat
     4561 gtccgataaa gggggcgtac catgaaacga tcgtttatct gcattttcac ttttgtttta
     4621 tttatctcca ttttttacgt tttaatgcat gcaggcagcc gggatgttcg ctcagcggca
     4681 gaaaccgtca tgaaagcttt tgaaatgacc ggtgccgaag ccacggcttc agagatatac
     4741 atcaggggca gatccgaaaa tcccggcttc aatgaagaaa tgtccctcag ccttctggtt
     4801 gagatcgttt caggcatcgg atccgggacc ggcggagctg tgcctgtttt ctgcccgata
     4861 gatactgatt ttgccattgg acacgaagta aattacataa tagatgaaaa taagaaaata
     4921 catatgacag tgcttgacca gaaggggccg gagaccggaa atacgctgct catcagcctt
     4981 tatgacattt ccagtgaacc tgaactgcaa aagtgctatg aggctgtcac agaagtgctg
     5041 gacaaataca gtatcgacca tgaaataaac ataactgtca caggattcgt gaaaggcaag
     5101 ctggacgatg atgaaacagc ggatattttc gacagagcca tgggcagcac aggtgcatca
     5161 agagtcgaag gcat
//
LOCUS       DAOSCC010000176         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10178_length_5138_cov_4.679323, whole genome shotgun sequence.
ACCESSION   DAOSCC010000176 DAOSCC010000000
VERSION     DAOSCC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_5138_cov_4.679323"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLG72_09020"
     CDS             complement(<1..315)
                     /locus_tag="PLG72_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ80973.1"
                     /translation="MTTFLIILAVIAVLAVIYVIASYNGLVSKRNKVANSWSQIDVQL
                     KRRFDLIPNLVETVKGYAAHEKDTYESVIRARNSYMSASSHQDVIKATNELSTGLNRL
                     MVL"
     gene            complement(386..2341)
                     /locus_tag="PLG72_09025"
     CDS             complement(386..2341)
                     /locus_tag="PLG72_09025"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOJ80974.1"
                     /translation="MKKGIKSAVFLLLVLILVSSVFCQSVSADSRYYKINAYTVYVVV
                     NPDGSADVEERIAYEFVGDFNGVLRDIDYEKTDGLENLTVYVEEDGISIKEFTVNSTT
                     GLDAAGASGTYNVYHEDEIAHLKVFEKSSNTRKTFVYKYRLKNVVTKYNDIAEFNRKL
                     IDTGWDVPLNDIRIHIQLPKGAFKDEIRVFGHGPLTGESKIIDGQNVDFILDRLDPGY
                     YVETLVLFPPRLVPESARVKAEDALQRILDNEKKMADEANAERERARKQVEEYNRRLA
                     EERERKQQRKKITDVLCIVLFIAWIPLIIRLYLKYDREFKSSFNAKYYRELPGEYTPA
                     EMSVLMNTGSVDKRDITATLMDLVRKGHLVLKQESYIKEGFFRDKEVEDYSLTLNPEA
                     PQSGLTGHESYLMNWFINEIGDGSRVFLDEVSDHVKTEADARRFTRNYQNWCERVKKE
                     AAKHNFFDESSRRGRIIAVLAGIAYIVLGFFLTAAAENPTGILVIISGFIMLIFGARL
                     NRRSAYGNEQYTMWKAFRNFLKDFSRLDKAEMPSIVLWEHYLVYAVSLGVAKEVIKQL
                     PLVYTDADLQNTRLTYMYGHNLRSLSSLTSTLDRTVYSVDHAIDRAMAVANSKQSSSS
                     GSGGGFSGGGFGGGSGGGGGRGGGGAF"
     gene            2621..3025
                     /locus_tag="PLG72_09030"
     CDS             2621..3025
                     /locus_tag="PLG72_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ80975.1"
                     /translation="MEHDAGQTAKQSGKGRKRPNFLLCAVIGLVTGLANGLFGSGGGT
                     IAVPAMAHLLKAEEHKAHATAISVILPLTVVSSVFYILKGHVDWGITIKATLGGLVGG
                     YIGAKLLNKCPDKLLRKIFAVFMAAAGIKMIF"
     gene            3188..3553
                     /locus_tag="PLG72_09035"
     CDS             3188..3553
                     /locus_tag="PLG72_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ80976.1"
                     /translation="MVLFLAGLASGIISGMGMGGGAVLIPVLVLVANPGQHIAQSVNL
                     LYFIPTAVVALVIHIRNKSIDFGMAVPIIAFGLAGALAGSWIAASMEAGLLRKIFGGF
                     LLAMSVYEMFRKGRNDAGK"
     gene            3622..3978
                     /gene="rsfS"
                     /locus_tag="PLG72_09040"
     CDS             3622..3978
                     /gene="rsfS"
                     /locus_tag="PLG72_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ80977.1"
                     /translation="MGNRKIADTAYGILEDKKARDIEVIDISEVSILADYFIICSGTS
                     TTHIKTLADELELKMGEAGYNLIHKEGYQSARWILLDFGQLVVHIFNEEDRKFYNLER
                     LWSEGKFRTPDDLEHS"
     gene            4022..>5138
                     /locus_tag="PLG72_09045"
     CDS             4022..>5138
                     /locus_tag="PLG72_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ80978.1"
                     /translation="MLAYSTKIDEKWQKKWEETGLYKFDRNNIDKKLYCLEMFSYPSG
                     AKLHVGHWYNYGLTDTWARLKRMQGWNMFHPQGFDAFGLPAENYAIKTGIHPKDSTYA
                     NIRTMEEQLRKMGASFDWDYEVITCAPEYYKWTQWIFLQLYKHGLAYRKKAPVNWCPS
                     CKTVLANEQVVDGCCERCKSEVTKKDLTQWFFKITEYAQELLDCLPQLDWPEKTKKIQ
                     QNWIGRSEGAEIVFEGADGSFSFKVFTTRADTLYGVTYVVLAPENELVDRITTPECRD
                     AVEQYREQAKKQTEIERMSTVREKTGVFTGAYAINPVNGDKVPIWIADYVLAGYGTGC
                     VMAVPAHDERDYEFAAKYNLPIKRVIKGGEGVDDTLPF"
BASE COUNT         1282 a         1230 c         1382 g         1244 t
ORIGIN      
        1 aaggaccatc agccggttga gccccgtgga aagctcgttg gtggccttga ttacatcctg
       61 gtgcgaagat gcgctcatgt aactgttgcg ggcccgtatt accgactcgt atgtgtcttt
      121 ttcatgcgct gcataaccct ttaccgtttc taccaggttt ggtatgagat cgaaccttct
      181 cttcaactgg acgtcgatct ggctccatga attggctacc ttgttgcgtt tgctgaccag
      241 tccgttgtag ctggctatca catagatgac tgccaggact gcgattactg ccagaatgat
      301 caggaaagtc gtcatttgga tatcctcctt aattaaaaat tgtacatttc ttatataaac
      361 atataatcaa actttatttc cggtatcaga acgctcctcc gccgccgcgg ccgccgcctc
      421 cgccggagcc tcctccgaag cctccgccgc tgaagccccc tcccgaacct gaagaggagg
      481 attgcttgga gttggctaca gccattgccc gatctatcgc atggtcaacg gagtaaacgg
      541 tcctgtcaag cgtgctggtg agcgagctca ggctgcgcag gttgtgacca tacatgtaag
      601 tgagccttgt gttctgcagg tcggcatcgg tgtatacgag aggaagctgc tttataactt
      661 cctttgcaac accaagcgaa accgcgtaga ccaggtagtg ttcccacagg acgatggacg
      721 gcatttccgc cttgtcgagc ctgctgaaat ctttcaggaa attcctgaaa gccttccaca
      781 tggtatactg ttcatttccg taagcagaac gcctgttgag ccttgctccg aagatgagca
      841 tgataaagcc cgatattatg accagtatcc ctgtcgggtt ttccgccgcg gcagtcagga
      901 aaaagccaag gacgatatat gctatgcccg caagtactgc aattatcctg cctctcctgc
      961 tggattcgtc aaagaaattg tgcttggcgg cctctttctt gaccctttcg caccagttct
     1021 ggtaattccg tgtgaatctt cgcgcatctg cttccgtttt tacatgatcg gacacttcgt
     1081 cgaggaacac gcgggagccg tctccgatct cattgatgaa ccagttcata aggtacgatt
     1141 cgtggcctgt aagaccggac tggggagcct cggggttcag cgtcaggctg taatcctcga
     1201 cctccttgtc cctgaaaaat ccctctttta tataagattc ctgcttcagt accagatggc
     1261 ctttccgcac caggtccatc agcgtcgcag tgatatcccg cttgtcgacg ctgcccgtgt
     1321 tcatgagcac ggacatttca gccggggtgt attctcccgg gagttcccgg tagtatttgg
     1381 cgttgaatga gcttttgaac tccctgtcat atttgaggta cagccggatg atgagcggta
     1441 tccaggcgat gaagagaacg atgcacaata catcggttat cttctttctc tgctgctttc
     1501 tttccctttc ttccgcaagc ctcctgttgt attcctccac ttgtttgcgc gccctttctc
     1561 gctcggcatt ggcctcgtca gccattttct tttcgttgtc gaggatcctc tggagcgcat
     1621 cctcagcctt taccctggct gattccggga cgagccgcgg agggaacagt acgagggtct
     1681 ccacataata acccgggtca agcctgtcga gtatgaaatc aacgttctgt ccgtcgatta
     1741 tcttagattc gccggtcaaa ggcccgtggc cgaacactct tatctcatcc ttaaaagccc
     1801 ctttcggcag ttggatatgt atccttatgt cgttgagagg cacgtcccag ccggtatcga
     1861 tcagctttct gttgaattcg gctatgtcat tgtattttgt caccacattc ttcaaccggt
     1921 acttgtagac aaaagtcttc ctcgtattgc tgcttttttc gaaaactttc agatgggcta
     1981 tttcatcttc atggtatacg ttgtaggtgc ccgatgcgcc ggcggcgtcc aggccggtag
     2041 tggaattgac cgtgaactcc tttatgctga tgccgtcttc ttccacgtac accgtcaggt
     2101 tttccagacc atcggtcttt tcgtagtcta tatcacgcag gacaccgtta aaatcgccta
     2161 cgaattcata tgcgatcctt tcttccacgt cggccgaacc gtcgggattg acgacgacat
     2221 agacggtata cgcgttgatt ttataatacc tgctgtccgc ggagacgctt tggcagaata
     2281 ccgacgatac caggatgagc acgagcagta aaaataccgc tgacttaatg ccttttttca
     2341 ttttgcgcac ctcacaagaa aatcacgctg accggatcca gcgatgcgga ccgggtcccg
     2401 ggccgctaac taaattctac cacattatac ttgacatgca atgtatttgt ttgttgaaaa
     2461 attatacagg ggacagagct ttcacgatat atttccggag gccaggatgc ccggtctccg
     2521 gatggccgcc gatcgacgtg gaaaatgaat aatcagggga taaaatcgaa caatatggat
     2581 agtacgatac agaaaaataa ggtaaggcag tgtaaacgtg atggagcatg atgcgggaca
     2641 aaccgcgaag caaagcggga aggggaggaa acgccccaat tttctgttat gtgcagtgat
     2701 cggcctcgtg acgggacttg ccaacggcct gtttggttca ggcggcggta cgatcgcggt
     2761 acccgcgatg gcgcacctgc tcaaagccga ggaacacaaa gctcacgcaa ctgcaatatc
     2821 cgtgatactg ccgcttactg tcgtaagttc ggtgttctat atcctcaaag gccatgtgga
     2881 ctggggcata acgatcaagg cgacgttggg aggcctcgtt ggaggataca tcggtgcgaa
     2941 gctgctgaac aagtgtcctg ataaattgct gaggaagata ttcgcggtgt tcatggcggc
     3001 agcaggcata aaaatgattt tttagaagga cctgcaaaaa ggccggcaga cggggagcaa
     3061 aaaagccaaa aagaatatga ggatgcaggc ggcaggagtt ttccagggta aattgcaata
     3121 aaggttttgt cggagcatca gaaaaggaga attgcaaggg gaagatgagt tgaacaactg
     3181 gaactggatc gttttattcc tggccgggct tgcgtcaggg atcataagcg gcatggggat
     3241 gggaggaggc gctgtcctga taccggtgct agtccttgtt gccaatccgg ggcagcacat
     3301 cgcccagagc gtcaacttgc tgtatttcat tccgacagca gttgtggcat tagtcataca
     3361 tatcagaaac aaaagcatcg atttcgggat ggcggtcccc atcatcgcat tcggcctggc
     3421 gggagcgctg gcaggttcat ggatagccgc atcgatggag gccggtctgc tcaggaaaat
     3481 tttcggcggg ttcctgcttg cgatgagcgt gtacgagatg ttcagaaaag gcaggaacga
     3541 cgccgggaag taatttttta ttcacaaacg gagcgcatta gtggtataat atttaatcaa
     3601 tcatgataca ggaggtacgc tttggggaac aggaaaatcg cagatacggc atacgggata
     3661 cttgaggaca aaaaggccag ggatattgaa gtgatcgata tttcagaagt atcgatactc
     3721 gccgattatt tcataatctg cagcggtacg tcgactacac atatcaagac gctggcggac
     3781 gaactggaac tgaagatggg cgaagccgga tacaacctta tccataagga aggttaccag
     3841 agcgcaagat ggatactgct ggatttcggc caactggtcg tacatatatt caatgaagaa
     3901 gaccggaaat tctacaacct tgagaggtta tggtccgaag gcaagttcag gacgccggac
     3961 gacctggaac attcatgatc ggatccggat cacaatttgc tcaatctgaa aggagctgtt
     4021 tatattggct tactcaacga agatagacga gaaatggcaa aagaaatggg aagaaacagg
     4081 tttgtacaag ttcgaccgca ataatatcga taaaaagctg tactgcctcg agatgttttc
     4141 atatccgtca ggagccaagc tccatgtagg ccactggtac aactacgggc tgacggatac
     4201 atgggcaagg ctgaagagga tgcagggctg gaacatgttc catccccagg gattcgatgc
     4261 attcggcctt cctgcggaga actacgccat taaaacaggt atccatccca aggattctac
     4321 atatgcgaat ataaggacaa tggaagaaca gctcaggaaa atgggcgcaa gctttgactg
     4381 ggactacgag gtgatcacct gcgcgccgga gtattacaaa tggacgcagt ggatcttcct
     4441 gcagctgtac aagcacgggc tggcgtacag gaagaaggcg cctgtcaact ggtgtccgag
     4501 ctgcaaaacg gtgctggcca acgagcaggt cgtggacggc tgctgcgaga ggtgcaagag
     4561 cgaagtaacc aagaaagacc tgacgcagtg gtttttcaag ataacggaat atgcgcagga
     4621 acttctggat tgcctgccgc agctcgactg gcccgagaag acgaagaaga tccagcagaa
     4681 ctggatcggc aggtcggaag gtgccgagat cgtattcgaa ggcgcggatg gcagcttttc
     4741 gttcaaagtg ttcacgacga gggcggatac gctttatggc gtgacctatg tggtactggc
     4801 gcctgaaaac gaactcgttg acaggatcac gacgcctgaa tgcagggacg cagtcgagca
     4861 atacagggaa caggcgaaga agcagaccga gatcgagagg atgtcgaccg taagggaaaa
     4921 gaccggtgtc tttaccgggg catatgctat aaatcctgtg aacggcgaca aggtaccgat
     4981 ctggatagcc gactatgtac tggccgggta cggcacgggc tgcgtaatgg ctgtgccggc
     5041 ccatgatgaa agggattatg agttcgctgc gaagtataac cttcctataa agcgtgtcat
     5101 aaaaggcggg gaaggcgtgg acgacacact gcctttcg
//
LOCUS       DAOSCC010000177         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10278_length_5099_cov_5.005353, whole genome shotgun sequence.
ACCESSION   DAOSCC010000177 DAOSCC010000000
VERSION     DAOSCC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_5099_cov_5.005353"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..968
                     /locus_tag="PLG72_09050"
     CDS             135..968
                     /locus_tag="PLG72_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ80979.1"
                     /translation="MYGLIIAFKDFKFSKGIIGSPWCGLDNFIYLFGLKDFYRVFGNS
                     FILSLIRLVFFFPIPIILALMLNEIPFMKYKRVLQTVIYLPYFISWVVIGGILVNLLS
                     PSWGIINSLIKVLGGEPIFFLGSDKYFRVIALISYIWKQAGWDTIIYLAAITSINPDL
                     YEAATIDGASRLQRIWHITMSGIKPTIIILLLLSIGNIMNNGFEQIYILQNSSNIQVS
                     EVFETYTYKLGMVNGRLSFATTVGLFSSVVGFILLMTTSVIIVMRLLWQIQKMIRWRR
                     S"
     gene            1551..2489
                     /gene="pfkB"
                     /locus_tag="PLG72_09055"
     CDS             1551..2489
                     /gene="pfkB"
                     /locus_tag="PLG72_09055"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOJ80980.1"
                     /translation="MIYTVTLNPAVDKTVVINDFKVDSVNRVSSLRTDAGGKGINVSK
                     VIKSLGGRSKAMGILGGSAGRYVKKYLDDMGIDNDFVFISGETRTNLKVIDGKNGTNT
                     DINEPGPNVSECELELLKDKLLDQVANGSIVVFSGSVPENVDKDIYFRWIDAVKQKGA
                     AAILDADGELLKKGIEAGPYLVKPNIYELGRLFGTDIKDIGKAVKYAETLIRDFAVGM
                     VAVSMGKDGALFVDKSRVALVHALKVNVQSTVGAGDSMVAALAYSIGKGMDYESMVRL
                     AAASASANVMTSGSQPADISVIRELEGRVTFEYLRS"
     gene            2646..3437
                     /locus_tag="PLG72_09060"
     CDS             2646..3437
                     /locus_tag="PLG72_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ80981.1"
                     /translation="MQKAVELASQGLTLRGMLHMPDNVSGKVPAVVIFHGFTGNKMEP
                     HFIFVKLSRMLEKKGIASVRFDFGGSGESDGDFIDMTLSGEIRDAHNILDFVKKLDGV
                     DPDRIGVVGLSMGGAVASVLAGERKDEIKTLCLWAPAGTMGEIIAKQVAGLGYDTAEK
                     IKKAGYVESGGFRVGIGFYEDVMKFDIFGKAKNFDKNVLIIHGEKDNAVPVEVSERYL
                     EYYGGRATLKIIKDADHTFNNSVWEKETLELTAEYLVKELKQTDD"
     gene            3499..4461
                     /locus_tag="PLG72_09065"
     CDS             3499..4461
                     /locus_tag="PLG72_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ80982.1"
                     /translation="MIQPYDLSLDELRKYKPALTRQPDFLEFWESNRKELAKVPLKYE
                     LEPYDYPAKGVKVSRVTFTGFMGASIDGWFAVPDAPGKYPGLVLYHGYNWAFDGCIHE
                     VVDMALHGYAVLQMLVRGQHGRSVDNTVSSLGHVAGWMTKGILSREEYYYRAVYMDSV
                     RALEVLADMDCVDDGRIGVTGGSQGGALTLVSAALSDIPAVAVASYPYLCNFDRAIDI
                     APSSPYGELNEFFRRYSDPAVEEQARKTLTYFDVMNLAPEIKCPVLVATGLVDEVTPP
                     STVFAAYNHMTCPKEIAVYRYFGHEFIPGFVEKKLRMLMKYLQD"
     gene            4591..>5099
                     /locus_tag="PLG72_09070"
     CDS             4591..>5099
                     /locus_tag="PLG72_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ80983.1"
                     /translation="MSEKAALNERPKAIILLVVTAVMWSLGGLLIKSIDAHPLAIAGV
                     RSAISAVIIVIATGRPKLNWSPAQIGAALSYAGMVTLFVAATKNTTAANAILIQYTAP
                     VYVIFLSAWLLKEKVNLFDWVTVIIVMAGMVMFFIDDISIGGMLGNILAAGSGISFAA
                     FTIFMRMQK"
BASE COUNT         1358 a         1056 c         1334 g         1351 t
ORIGIN      
        1 aatctctgga ctggcaatac cgtcagctta ctgacatttt gagatagcga aaacagtact
       61 tctcaaacaa taagtattta tatctcttgt tacttccctg cgttatatac tttataatat
      121 ttaactattt gccgatgtac ggactgatta tcgcattcaa agattttaag ttttcaaagg
      181 gcatcattgg gagcccgtgg tgcggactgg ataattttat ttatcttttt ggactgaaag
      241 acttctaccg ggtctttgga aattcattca tcctaagtct gataagactg gtattctttt
      301 tcccgatacc gatcatactt gcgctgatgc tcaatgaaat tccttttatg aaatataagc
      361 gggtattaca gactgtgatt taccttccgt actttatttc atgggtggta atcgggggta
      421 ttctggtaaa cctgctatcc ccttcctggg ggataatcaa cagtttgata aaggtattgg
      481 gcggagagcc gatttttttc ctgggcagtg acaaatactt ccgggtgatt gcccttattt
      541 catatatatg gaagcaggcc ggttgggata ctattattta tcttgcagcg attacgtcta
      601 taaacccgga tctttatgaa gctgcaacaa tcgacggcgc gtcaaggttg cagcgtatct
      661 ggcatattac gatgtccggc attaaaccta caataatcat tctcttgctt ctttcgatcg
      721 gaaacattat gaacaatggg tttgaacaaa tatatatact tcagaacagc agcaatattc
      781 aagtctccga agtctttgag acttatacct ataaattggg tatggtaaac ggacgcttat
      841 cttttgcgac aacggtaggt ttattcagct ctgtcgtagg tttcattctt ctcatgacca
      901 cgtcggtaat tattgtgatg cgcctcttat ggcaaataca aaaaatgata cggtggagaa
      961 gaagttaagc tacacttaca tagggcattt cagccgttat atcaaacccg gtgctttccg
     1021 gatagccagt actgtatata ctgacaggct tgaaacgaca gcgttcagga atccggatgg
     1081 tacgattgca tgtgtattgc tgaacaggac tgaaagcgac ttgcctgtta acttaagaat
     1141 atgcggtcag tttgcttata tgctgattcc gcgggaatcc attaccacac tgattattac
     1201 ggataaaaaa atataacaat ggcttttatc tgtgaatctc tgaatcgact accttatatg
     1261 tcaagcgtgt catcacgcct gacatataag gcctctttcc ttcataaatt cgcgattctg
     1321 cagaactgtc cctcccctct cataactgtt tttcttttca ggttactgtt ggtttcgcag
     1381 aactgtcccc ggattccctc gaattgtttc cgacttttct cttttcgatg gactattcct
     1441 cttttctcct cgccagggaa ccctcgattt tgcagaactg tcccccagac aattgacatg
     1501 ccgtgttatc aattctaaaa ttaagtcaaa cgcctgattc ggagtaaaaa atgatatata
     1561 cggtcacgct gaatccggct gtcgacaaga cagtcgttat aaatgatttt aaagtggact
     1621 cagtcaacag ggtttcttct ttgaggacgg atgccggcgg caagggcatc aacgtttcca
     1681 aagtcatcaa aagccttggc ggacgcagca aggcgatggg catccttggc gggtctgccg
     1741 gaaggtatgt taaaaaatac ctggatgata tgggcattga taatgatttc gtgttcatca
     1801 gcggtgaaac gaggaccaat cttaaagtca tcgatgggaa aaacggaacc aataccgata
     1861 taaatgaacc tggcccgaat gtctcagaat gtgaactgga gttactgaag gacaaattgc
     1921 tggaccaggt ggcaaatggt tcgatcgtcg tattttccgg gagtgtgcct gaaaatgtgg
     1981 ataaggatat atatttcagg tggatcgatg ctgtgaagca aaaaggagcg gcggcaatac
     2041 tcgatgcaga cggtgaactg ctgaagaagg gtatcgaagc gggaccgtac ctggtaaagc
     2101 ctaatatata tgaactggga aggcttttcg gaactgacat caaagatatc gggaaggccg
     2161 taaaatatgc tgaaacgctt atcagggatt tcgctgtcgg gatggtcgcc gtttcgatgg
     2221 ggaaagatgg agcccttttt gtcgataagt caagagtggc gctggtccac gctttgaagg
     2281 ttaatgtgca gagcactgtg ggggccggcg attccatggt cgcggcgctt gcctattcca
     2341 tcggcaaagg aatggattat gaaagcatgg tcaggcttgc agcagcttcg gccagtgcaa
     2401 atgttatgac ttcaggttcc cagccggcag atatctctgt tatcagggag cttgaaggcc
     2461 gtgtcacatt cgagtatctg agatcatagc gaaagattat aatggggcaa cggatgtgct
     2521 gcgggtatct gctgtggaag attatggcct ggatatgttg aagatttatt ctgtatgcag
     2581 tgagtatcga tattacctgt aattgaacaa ttttaatcca tgatacttga aagggtggtg
     2641 cagcaatgca gaaagcggtc gaactggcaa gccagggact tacattgagg ggcatgctcc
     2701 acatgcctga caacgtttcg ggaaaggtgc cggcagtggt catattccac ggattcaccg
     2761 gcaacaagat ggaacctcac tttattttcg ttaagctgtc caggatgctt gagaaaaaag
     2821 gcattgcaag tgtcaggttc gattttggcg gaagcggcga aagcgacggc gatttcatcg
     2881 atatgacgct ttccggggag atccgcgatg cccacaatat actggatttt gtgaaaaaac
     2941 tcgatggcgt cgatccggac cggataggcg ttgttggact gagcatgggc ggcgcagtcg
     3001 caagtgtcct ggccggcgaa agaaaggacg aaataaaaac cctttgcctg tgggcgcctg
     3061 caggcaccat gggagaaatt atcgcgaaac aggtcgccgg acttggatat gacacggccg
     3121 aaaagatcaa aaaggccggg tatgtggaat caggaggatt tcgcgttggt atcggctttt
     3181 atgaagatgt gatgaaattt gatatttttg gtaaagcaaa gaactttgac aagaatgttc
     3241 tgataatcca tggggaaaag gacaatgctg ttccggtcga ggtttctgaa agatacttgg
     3301 aatattatgg cggaagggcg acgctgaaaa taattaagga tgccgaccat acattcaaca
     3361 attctgtctg ggagaaagaa acacttgaac tgaccgccga atatctcgta aaagaactga
     3421 aacagacaga tgactgataa agagaaacct gaacagaaaa aagcaatatg tacagggaaa
     3481 atgcggaggt gtgcataaat gatacagcca tatgatctta gccttgatga attgcggaag
     3541 tacaagcctg ccttgacgag acagcctgat tttcttgaat tctgggagtc gaacaggaag
     3601 gaacttgcaa aggtgccgct gaaatacgaa ctggagcctt atgactatcc tgcaaaaggt
     3661 gtcaaagtca gcagggtgac gtttaccggg tttatgggcg caagcataga cggatggttc
     3721 gccgttcccg acgcgccggg taaatatccg ggccttgtgc tgtatcatgg gtataactgg
     3781 gctttcgacg gatgcataca tgaggtagtg gatatggcgc ttcatggtta tgctgtgctg
     3841 caaatgctcg tccgtggaca gcatggccgc agtgtggaca atacggtatc gtcgcttggc
     3901 catgttgccg ggtggatgac caagggaata ttgtccaggg aagagtatta ctatcgtgcg
     3961 gtttacatgg acagcgtccg cgctctcgaa gtacttgcgg atatggactg tgtcgatgac
     4021 ggcaggatag gagtaacggg aggcagccag ggcggagcgc ttacgctggt ttcagcagct
     4081 ctttcggaca taccggctgt tgccgtggcg tcataccctt acctgtgtaa ctttgacagg
     4141 gctatcgata ttgctccttc gtcgccgtat ggcgagctga atgagttttt ccgcaggtat
     4201 tccgatccgg ccgttgaaga gcaggccaga aagacgctga catactttga tgtcatgaac
     4261 cttgctccgg aaataaaatg cccggtactc gtggcaacgg ggctggtcga tgaggtgacg
     4321 ccgccgtcga cggtgtttgc cgcatacaat catatgacat gtccgaaaga gatcgctgtt
     4381 tacaggtatt tcgggcatga attcataccc ggatttgtag agaagaaact gaggatgctg
     4441 atgaagtatc tgcaggatta ggaacttgat gacgcataca actttaatga tcaggccggt
     4501 ggcatccaaa acctgccgga ccgatcatac cggtattcgg ccgtatcggt gtgaatacag
     4561 gaccgtcccc tgaaaacaag tggtgattac gtgagcgaaa aagcagcttt gaatgaaaga
     4621 ccaaaagcaa taatactcct cgttgtaacg gcggtgatgt ggagcctcgg aggactgctg
     4681 ataaaatcca tcgacgccca tccgctggcc atcgccgggg tcaggagcgc gatatctgcc
     4741 gtcattatag tgatcgcaac cggcaggccg aagttgaact ggtctccggc tcaaatcggc
     4801 gcggcgctgt cgtacgccgg catggtgaca ttgttcgttg ccgcaacgaa aaacacaacg
     4861 gctgccaatg ccatactgat ccagtatacc gcccctgtat atgtgatatt tctcagtgcg
     4921 tggctgctga aggaaaaagt gaacctgttc gattgggtga cggtaataat cgtgatggcc
     4981 ggcatggtca tgttcttcat cgacgatata agcattggcg gtatgctcgg gaatatcctt
     5041 gctgcgggaa gcgggatctc gtttgccgca ttcacgatct tcatgcgcat gcaaaagga
//
LOCUS       DAOSCC010000178         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10420_length_5051_cov_3.514011, whole genome shotgun sequence.
ACCESSION   DAOSCC010000178 DAOSCC010000000
VERSION     DAOSCC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10420_length_5051_cov_3.514011"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1049
                     /locus_tag="PLG72_09075"
     CDS             204..1049
                     /locus_tag="PLG72_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ80984.1"
                     /translation="MKQKYLVTHSLNHTDLNMYQCGTEVCAPGHYYGPAVRDHFLIHY
                     IHSGRGRFCVGDKVYHLSKGQGFLICPNIVTFYQADMDDPWNYSWVGFHGLKAESYLK
                     SAGLTADNPIFTTNDDEFIGGCFRKMIEAKNLAKGREIRLLGHLYLFLSQLIEENGTG
                     MFADTAGSRKEAYVKRAIEYIDMNYSRKMTIAELAHSIGLDRSYLGSIFKEHFGVSLQ
                     GYLKNYRMDKACELMKNEDLSIGDIARSVGYDDPLLFSKLFRKCKGVSPREYRKMYID
                     SYNAG"
     gene            complement(1174..1683)
                     /locus_tag="PLG72_09080"
     CDS             complement(1174..1683)
                     /locus_tag="PLG72_09080"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOJ80985.1"
                     /translation="MARDIFLLAALMCAAINIWSGGKAWSVIVIWALYMIWSMLVYPD
                     MIEYNRISQFTKAIVQSCVMLVLIDVFITSGWAANVVSIICFGSLIAVCLLLYTDFER
                     QRHNLLPVILLSALSLITSAVLMIISGGNIRWQLPVTCVLSFVVLAVCMKTMGWNIKS
                     ELKKMFHTI"
     gene            complement(1739..>2878)
                     /locus_tag="PLG72_09085"
     CDS             complement(1739..>2878)
                     /locus_tag="PLG72_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80986.1"
                     /translation="PEILQMALTFTIKRFPSFATTVKKGFFWHYLDSTKRRYIVEPET
                     GIPCQPIHIGRSGSQTFRVLYYRNRISVEYFHILTDGTGGMCFLKTLVAEYFRLLGTE
                     SACVSGVLPVNGLVSPGEIANEFINIRSEGPASSFVDKAAVQYGGRLSRIRPCRVIHF
                     KIDAAALKAVADRKRATVTAYILSLMFVAGKKATDEVAGEFNIQVPVNMRKFYPSETL
                     RNFSMYCGIRIPLAEISEPDALIPEIMRQLTEKASCQAMNEMVKATNRIVNAIRFIPL
                     FLKAPVVRMVYGFIGDRNFSNTLSNLGVVSLPPEIAGLVDSMDVVLGAAITNRASCSL
                     VTCGNTSTLSVSKNTADPSFEEALYELLCRDGLTPVVEGSELIAD"
     assembly_gap    2879..2978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2979..3178)
                     /locus_tag="PLG72_09090"
     CDS             complement(<2979..3178)
                     /locus_tag="PLG72_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80987.1"
                     /translation="MVKRYMSFAMRKKETAQKPNISTACKEKQKDGRKGVKKRYSQSI
                     SLPFARKADNQKAALFFEDLECR"
     gene            complement(3925..>5051)
                     /locus_tag="PLG72_09095"
     CDS             complement(3925..>5051)
                     /locus_tag="PLG72_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ80988.1"
                     /translation="FFIDRFGKSLGKLGKAEWEASRKTNLIELAMRGFTVAGYGGNLY
                     MLVKALLAGEISVGAFAAVFGSIGMMFGSMEEVIARHIGNMASNLGKAQNFIRFMELP
                     ERGGEEDTADFSEGIVLENVSFIYPNAECKSIDNVSFEIKAGETVAIVGENGAGKTTL
                     IRLILGLYKPTEGVVRINGMDTSKVNSRSLFRNVSGVFQKYQRYLMTLEDNIRISDME
                     SSEEVSAAAEQADVEADGASFPDGYKTMLSREFDGVDLSGGQWQRIAIARGLYRVHDI
                     IVLDEPTAAIDPLEESRIYKKFVEISRGKTAIIVTHRLGSTKIADRVIVMDKGRVAAI
                     GTHDELMENCELYRTMYNSQAEWYSDKLLHNGAGALTAAG"
BASE COUNT         1200 a         1347 c         1135 g         1269 t
ORIGIN      
        1 gaatgacagg atgtccctta caagatatct ggtgaagccg aaactgccgg ccccgataaa
       61 ggctattttt ctcatggaaa cgccccctta agcaagtgtt gtatacctgg tactttcatt
      121 ttataccaca atctttgtac agccaatggc agcatgcttg taaaatatgt tataatgctt
      181 ggtgagaaag aaaggatccg gaaatgaaac agaagtattt agtcacccat agcctgaacc
      241 ataccgatct gaatatgtat caatgcggta ctgaggtctg cgcccccggg cattactacg
      301 gacccgccgt cagggaccat ttcctcatcc attatatcca cagcggacgg ggacggttct
      361 gcgtcggcga taaggtatac cacctttcca aaggacaggg gttcctgatt tgtccgaata
      421 tcgtcacatt ttaccaggct gacatggatg acccgtggaa ctactcatgg gtggggttcc
      481 acgggctgaa agccgaaagc tatctgaaaa gcgccggcct gacagcagac aaccccattt
      541 tcaccacaaa tgacgatgaa tttatcggcg gatgtttcag gaaaatgatc gaagccaaaa
      601 acctggcaaa aggacgggag atccgcctgc tcggccacct gtacctgttc ctttcacagt
      661 tgatcgaaga aaacggcaca ggtatgttcg ccgatacggc cggttcccgg aaagaagcat
      721 atgtgaagag ggccatcgag tacatcgaca tgaattactc gagaaagatg accattgctg
      781 aacttgcgca cagcatcggc ctcgacagga gttacctggg cagcatcttc aaggaacact
      841 tcggcgtctc tctgcagggt tacctgaaaa actaccggat ggacaaggca tgcgaactga
      901 tgaaaaacga agacctgtcg ataggagaca tcgcgcggtc tgtaggttat gacgacccgc
      961 tgcttttctc caagctgttc aggaaatgca agggagtttc tccgcgggag taccgcaaga
     1021 tgtacatcga cagttataat gccggctgat ttccccgtcc cggcccaagc tggggcccaa
     1081 tcacagctga accacattcc tgtcattgat cctgccaggc aaacatgtgt cagcactgga
     1141 ctgcaaatat caccatgcgg tccgccgacc ctgtcatatg gtatggaaca tctttttcaa
     1201 ttcgcttttt atattccacc ccatcgtttt catacacacc gcgagaacaa cgaaggacag
     1261 tacgcacgtt acaggaagct gccagcggat gtttcccccc gaaatgatca tcagcaccgc
     1321 cgatgtgatc agtgaaagcg ctgacagcag tataaccggg agcaggttgt gcctttgcct
     1381 ttcgaaatcc gtatacagca gcaggcaaac tgcgatcagt gagccgaagc atattatcga
     1441 aactacgttt gccgcccatc cggatgtaat gaaaacatcg atcaggacaa gcatgacaca
     1501 gctttgcaca atagccttgg taaactggct gatccgattg tattcgatca tatccggata
     1561 aaccagcatc gaccatatca tgtaaagcgc ccagatcact atgacgctcc atgccttgcc
     1621 gcccgaccag atgtttattg cagcgcacat aagcgcagca agcaggaata tgtccctcgc
     1681 tataccgatt attatgctgc ggtggctttt cctcttctat ggtttcggat atgtaacatt
     1741 aatctgcaat gagctcactc ccttcaacga ccggagtaag gccatcccgg caaagaagct
     1801 cgtacagcgc ttcttcaaat gacggatccg cagtattttt ggatacggac agcgttgagg
     1861 tatttccgca agtcaccagg gagcaactcg cacggttggt aatcgccgct cccagtacta
     1921 cgtccatgct gtccaccagt cctgcgattt ccggcggcaa tgatacaaca ccgaggttgg
     1981 aaagcgtgtt gctgaaattc cggtctccga taaagccata taccatcctt acaacaggcg
     2041 ccttgaggaa aagtgggatg aacctgatcg catttacgat cctgttcgtt gctttgacca
     2101 tctcgttcat agcctggcag gaggctttct cagtaagctg gcgcatgatc tccgggataa
     2161 gcgcatccgg ctccgagatc tccgccagcg gtatccgtat accacagtac atggaaaagt
     2221 ttctcagggt ctccgaggga tagaattttc tcatgttcac agggacctgg atgttgaatt
     2281 ctccggcaac ttcatcagtt gcctttttcc ctgcgacaaa cataagggaa agtatgtacg
     2341 ccgtgacagt cgcacgcttt ctgtcagcga cagccttcag tgcggcagca tcgatcttga
     2401 agtggatgac cctgcaaggt cttatgcggg acagcctgcc gccatattgc acggcagctt
     2461 tatcgacaaa gcttgaagca ggaccttcgg aacggatatt gataaactcg tttgcaatct
     2521 cacccgggga taccaggcca ttgacgggta aaacaccact gacgcaggca gactcagtgc
     2581 cgagcagccg aaagtattcc gcgacaagag tcttgaggaa gcacataccg cccgttccgt
     2641 ccgtcagtat gtggaagtac tcaacactga tgcggttccg gtaataaagg acgcggaagg
     2701 tctgcgaacc gcttcgccct atatggatcg gctggcaggg tatgcctgtt tccggttcca
     2761 cgatatacct gcgcttcgta gaatcaagat aatgccagaa gaagcctttc ttgaccgtcg
     2821 tcgcaaagct ggggaaccgc ttgatcgtaa aagtcagggc catctggagt atctccggnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcattccagg tcttcaaaaa
     3001 acagggcagc tttttgatta tctgctttcc tggcaaatgg caggctgata ctctgcgagt
     3061 accgtttttt cacgcctttc cttccgtctt tctgcttttc cttgcaggcc gtcgaaatat
     3121 tcggtttttg cgcagtttct ttttttctca tagcaaatga catatacctc ttgaccatat
     3181 cggtttcctg caagccgctt ttgaaacctt aatataatct tgtccttatg agcgatttca
     3241 aatcatatct ttgcctgtat tttccgaaat ttctccactt tggtatgcaa aaagttctgc
     3301 tgaggtatac tttaccacaa cagaacttcc catatcaaat ttttatggcc ttttcccgta
     3361 ttgtgcctgt caagtctctt caggcgatac tttttcataa cgccttccgc tgctataccg
     3421 gcaacggtac ctgtatacca ccgctatgtc tgccggttcc ggtccgtgcc tgtgaatctt
     3481 ccggcgatgt tttcttactt acgacacctg catgccacgc ctctgctgtt ccgtttttcc
     3541 atttatgggc tggttttttc aaaattacag cgttttctgt accattttta catatatggg
     3601 cgcttggatt caaatttact ccacgtatac gttacataaa ctccgttaat tgtatcttat
     3661 gtaaacactt tacgcttaat cgaacgcaac caggtggttt attgttcaat aaacgaaaat
     3721 ttggtacata aaacatatcc ggagttaatt ctgtggtttt cagccgacca ctctgtcggc
     3781 atcgcggcag gacaacaggc ctcgtggcag ggcagccgtc ccgtatctgc accggcggca
     3841 ggacaaacgt taccgtatct gcaccggcag caggataacc gtctccatgt ctttaccagg
     3901 cagcagcaga atcgtcccaa cgcctcagcc cgcagcagtc agcgcaccgg cgccgttgtg
     3961 cagcagcttg tcagagtacc attcggcctg tgaattatac attgtcctgt acagttcaca
     4021 attctccatc agttcatcat gcgtccctat ggcggcgacc ctgcccttat ccattactat
     4081 gacccggtcg gctatcttcg tagaacccaa cctgtgggta acgataatgg ctgttttgcc
     4141 cctggagatc tcaacgaact tcttatatat gcggctttct tccaaaggat cgatggccgc
     4201 tgtaggttca tccaggacta tgatatcatg cacacggtaa agaccccttg cgatcgctat
     4261 gcgctgccac tgcccaccgg acaggtctac gccgtcgaac tccctcgaca gcatggtctt
     4321 gtagccatcc ggaaaactcg ccccatcagc ctcgacgtcg gcctgttcgg ccgcggcact
     4381 gacttcctcg gagctttcca tgtcactgat ccgtatattg tcctccagtg tcatcaggta
     4441 ccgctggtat ttctggaaca ccccggaaac attgcggaac agggaccggc tgttcacttt
     4501 agatgtgtcc atgccattta tcctgaccac tccttccgta ggtttgtaca gccccagaat
     4561 cagcctgatg agagtagtct tccccgcgcc gttctcgcct acgatggcta ccgtctcgcc
     4621 ggcttttatt tcaaacgaca cattatcaat actcttgcat tcggcattgg ggtaaatgaa
     4681 gctgacattc tccagcacga tgccttcgct gaaatcggca gtgtcttctt ccccgccgcg
     4741 ctccggcagt tccatgaaac gtatgaaatt ctgcgctttg ccgaggttgg atgccatatt
     4801 gcctatatgc cgtgctatta cttcctccat actaccgaac atcatgccaa tggaaccgaa
     4861 tactgcagca aatgcgccta cagatatttc acctgccagc aatgccttta ccagcatgta
     4921 gaggttacca ccgtatccgg caaccgtgaa cccacgcatg gccagctcta tcaggttggt
     4981 tttcctgctt gcttcccatt cagcctttcc cagcttgcca agcgacttgc caaaacggtc
     5041 gatgaagaac c
//
LOCUS       DAOSCC010000179         5048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10426_length_5048_cov_5.314040, whole genome shotgun sequence.
ACCESSION   DAOSCC010000179 DAOSCC010000000
VERSION     DAOSCC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10426_length_5048_cov_5.314040"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..979
                     /locus_tag="PLG72_09100"
     CDS             389..979
                     /locus_tag="PLG72_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ80989.1"
                     /translation="MDFKESRTYVNLMRAFAGESQARNRYNISASYARKEGLQIIEAI
                     FNYTADQERAHAKVFYELLKEAAGETIAIDGTYPVDLYDSTLELLRAAQHNENEEWED
                     VYRNFGLVAKEEGYAKIAMTFENIAKIERVHAERFGRFADALENGTLFKKDEEIAWLC
                     TNCGHIHIGREAPEVCAVCKHPRGFFIPFVESFAAR"
     gene            complement(1271..1477)
                     /locus_tag="PLG72_09105"
     CDS             complement(1271..1477)
                     /locus_tag="PLG72_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80990.1"
                     /translation="MKFKVTLGCAACGYSDTVEADTVFDCSGLVCPKCKNEDCLSITA
                     VSETTADTFKKQNILQRLKMIQNL"
     gene            complement(1741..3018)
                     /locus_tag="PLG72_09110"
     CDS             complement(1741..3018)
                     /locus_tag="PLG72_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOJ80991.1"
                     /translation="MKKILCLLLAVSLMACNAGCSSGSASDRENESMTDPNSVIKDTS
                     AQAGPKAPGVKSQGLSEANRQFAFDIFKQLSKEDKNQNIFISPLSISVALSMAYQGAD
                     GSTKTAMENALGYDGINKDDLNESYRKLLGYLNKVDKNVELNISNSVWIRDGKPVSEE
                     FLETNEDVFGAKAAVIDFSGKNAADTINGWISKSTKGKIDKIISPPINQDVLMYLINA
                     IYFKGHWTKKFDTRNTFSAEFSNADGTKSKVMMMSREGRVEYASGENYKAVRLPYGSG
                     KTAMYLILPEEGTDLDEFIAGLRPEFWGTLKSSLSATDDVKLQVPRFKLEYGAKELRE
                     SLSALGMGEAFSGTADFSGIRENIYISSVLHKAVIEVNEEGSEAAGVTSVEVQFTAAA
                     LEPVSFIADRPFIFAIAEDDTNTILFLGKVIRL"
     gene            3246..3959
                     /locus_tag="PLG72_09115"
     CDS             3246..3959
                     /locus_tag="PLG72_09115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOJ80992.1"
                     /translation="MGHNSGRQSNKFEICAEDITIEVERKKIKNMHLRVYPPDGHVRV
                     SAPMRCSEDTIRSLVASKIDWIKKHQERIRRRNTRAPLKFSDGELHFFKGKEYRLRVI
                     EKEAKPHVETNGDSLVLYVRPGADEEKRRAVLDEFYRYQLNQELPGIIDRWAKKMNVR
                     VSGFGIRKMKTKWGTCNIKARRIWINLELAKKPPECLDYIVVHELAHLLERYHNSTFY
                     GYIDRFLPQWRIYKKELDS"
     gene            4256..>5048
                     /locus_tag="PLG72_09120"
     CDS             4256..>5048
                     /locus_tag="PLG72_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOJ80993.1"
                     /translation="MDYRKESLRLHNEWKGKIEVISTVPVTNKQELSLAYTPGVAEPC
                     LAIQKDVDLSYELTRRWNLVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKSFADVD
                     AFPLCIRSKDVDDIVKTIKLISGSFGGINLEDISAPRCFEIERRLKAECDIPIFHDDQ
                     HGTAVVTLAAMINALKIVKKDIRDIEVVINGSGAAGIAVTRLLMAMGLKKVILCDTKG
                     AIYEGRENLNAEKAEMAKISNFEKKKGTLKDVIVGADVFIGLSVAG"
BASE COUNT         1307 a         1156 c         1270 g         1315 t
ORIGIN      
        1 attttcaaaa gcagttccga cagtttcttg ctgacggtat cgttgattat acctgccttt
       61 ttggcaatga aaccgataaa cataataatg aatagtatga gtacctgatt gaaaacagtc
      121 accgagttgc ccatatgtac ctcacagtat gcagatttga ccgtgttaac agccttagtc
      181 gatattatac tacacaaatc cttaaaagta atacagaaat ccggatgtgt atcaaacttc
      241 catcatggat gcagtccccc gtacggaaca gaatggcgga ataattttgc ttttcttaca
      301 gataatatcc taataaaatg cagtatattc ccggaatgat cctttgtacg gatcattccg
      361 gacaaaacag caagaaggag cagtgaagat ggattttaag gaaagcagaa cttacgtcaa
      421 tctgatgagg gctttcgcag gcgaaagcca ggcaaggaac aggtataata tctcggcgtc
      481 atatgccagg aaggaaggcc tgcagatcat cgaagcgata ttcaattaca ctgcggacca
      541 ggagagggcg catgcaaaag tgttctatga actgttgaag gaggcggcgg gagagactat
      601 cgccatagac ggaacctatc cggtggatct gtacgacagc acgctggagc tgctgcgggc
      661 ggcacagcac aatgaaaatg aagaatggga agatgtgtac aggaatttcg gcctggtcgc
      721 aaaggaagaa ggatatgcaa aaatcgccat gacattcgag aacatagcca agatcgaaag
      781 ggtacacgca gaacggttcg gaagattcgc cgatgcactg gagaacggaa cactgttcaa
      841 aaaagatgaa gagatagcct ggctctgtac caactgcgga catatccata taggcaggga
      901 agcgcccgaa gtatgcgccg tgtgcaaaca tccgcgtggc tttttcatcc cgtttgttga
      961 atcctttgca gccaggtaaa tttgcgggat aatcagtatt gcctgatgca attcaaacat
     1021 gtccgtgcgt aacaaatctg tccgatagtc gtgcagattc cgcagtatgg gcaagtcatg
     1081 tgttgatcca tatataggag acaaattcgc agcgggtact gatttgttgc cctgggatcg
     1141 ttatgcggcg gcaaatcggt gcctgttttc ctggtgtcct gttggtttct gctttctgca
     1201 tatccatata catatatggc ggcagccgcc gctttgccac tgcagccatc aggctgcatc
     1261 tgcgcctgca tcagagattt tggatcattt tcaggcgctg gaggatattt tgctttttga
     1321 aggtatcagc agtcgtctcc gacactgctg taatcgaaag gcagtcctca tttttgcatt
     1381 tcgggcagac gaggcctgag cagtcgaaca cggtatcggc ttcgacagta tcgctgtatc
     1441 cgcatgcggc acagccaagt gtgactttga atttcatatg gttacctccg gtggttgcct
     1501 cctgatgtgc cgaacatgtg ttcgggattt aattatacat ctgccgtgat ggataataca
     1561 ggccggtatt ttgccatacc ggcctgtata aatcaaatat tttcacatgg caagcggtga
     1621 catggtttac cttattattt cacatagttt tgcatgttca gtgtataccg gaccctattt
     1681 gcatactgcc gactcatctt acggactgtc ctgcttcgct tgcgcccggg ccggtttccc
     1741 tcacagcctg atgactttac cgaggaaaag gatcgtattc gtatcatctt cagcgattgc
     1801 gaatatgaaa ggcctgtctg ctataaaact taccggttcc agtgccgctg cggtgaactg
     1861 tacctcaact gaagtaactc cggctgcttc gctgccctct tcattcactt cgatcacagc
     1921 cttgtgaagg acactgctga tatatatgtt ttcccgtatg cctgaaaaat ccgcagtgcc
     1981 gctgaatgct tcgcccattc cgagcgcaga aaggctttcg cgcagttctt ttgcgccata
     2041 ctcaagcttg aatcgcggga cctggagctt cacatcatca gttgcgctga gactgctttt
     2101 tagcgtgccc cagaattccg gccgcaggcc cgctataaac tcatcaaggt ccgtgccttc
     2161 ttccggcagt attaaataca tggcagtctt tccgctccca tacggaagcc ggactgcttt
     2221 gtaattttcg cctgaagcgt attccaccct gccttcccgg ctcatcatca tgactttgct
     2281 ttttgtaccg tctgcattgc tgaattcagc tgagaatgtg tttctggtgt cgaatttctt
     2341 tgtccaatgt cccttgaaat atatcgcatt gataaggtac atcaggacat cctggtttat
     2401 cgggggactg atgattttat ctattttacc ttttgttgac ttagatatcc atccattgat
     2461 tgtatctgcg gcatttttgc ctgaaaaatc gatgacggcg gccttggcgc cgaaaacatc
     2521 ttcgtttgtt tccaggaatt cctcgctgac tggctttccg tctcttatcc atactgaatt
     2581 gctgatattg agttcgacgt ttttatcgac tttgttaaga tatcccagga gtttcctgta
     2641 tgattcattg agatcatcct tatttatacc gtcgtatccc agcgcgtttt ccattgcggt
     2701 tttcgtcgat ccgtctgcgc cctggtacgc cattgacagc gcaacagata tgctcagcgg
     2761 cgaaatgaaa atgttttggt ttttatcttc tttgctcagt tgtttgaata tatcgaatgc
     2821 gaactgacga tttgcttcgc tcaggccttg tgatttgacg cctggcgctt tcggcccggc
     2881 ttgggctgaa gtatccttta ttacgctgtt tggatctgtc atactttcgt tttcacgatc
     2941 cgatgcgctt cccgatgaac aaccggcatt acacgccata agcgaaactg ccagcagcag
     3001 gcacagaatt tttttcataa ttgcatacct ccttaacatc tatttttgac gagcaaaacc
     3061 agtttatgtt tcattgcata ccgaccggca ttatggagcc cgaggtgctg ttatctcccg
     3121 cattgttaac tgcagatgcg aagaaacctg taaaatcccc cggaacggaa aatttcgccg
     3181 gaccggaacg ccagggacgc cggaagtgtt ataatatcca caaagacgaa tgagagggta
     3241 tatggatggg gcataacagc ggcagacaaa gcaacaaatt tgagatctgt gcggaagata
     3301 tcacgataga ggtcgagcgg aaaaaaatca aaaacatgca tcttcgggta tatccgcctg
     3361 acggacatgt ccgcgtatct gcacccatgc gctgcagcga ggatacgatc cggtcccttg
     3421 tggcctcgaa aatcgattgg ataaaaaagc accaggagag gatcaggcgg aggaataccc
     3481 gcgcaccgct gaaattttcc gacggcgaac tgcatttttt caaagggaag gaatacaggc
     3541 tccgggtaat cgagaaagag gcaaaaccgc atgtggaaac aaacggcgat tcgcttgtgc
     3601 tttacgtccg ccccggggcg gacgaggaaa agcgccgtgc cgtacttgac gaattttaca
     3661 gatatcagct taatcaggaa ctgcctggta ttatcgacag gtgggcaaag aagatgaatg
     3721 tcagagtcag cggtttcggc atcagaaaaa tgaaaaccaa gtggggaacc tgcaatataa
     3781 aagcccgccg gatatggatc aacctcgaac ttgcgaagaa accgccggag tgcctcgatt
     3841 atatagtggt acacgaactg gcgcatttgc ttgaacgata ccataacagc acgttctatg
     3901 gctacattga ccggttcctg ccgcagtggc gtatatataa aaaggaactg gactcgtgat
     3961 attccgggaa tgcaccggcc ggcatgccgc agcgccagtc gtcatactgc cgcgctgatc
     4021 ctcccgatta tacggttttt cgcaggtgag atccagcttg tggcgtattt catcgctgct
     4081 tgccaaaaat agcttgatta aggatataaa ctttgctatt cttgttagtg tttatgttaa
     4141 gcagttatca ctgcagtgtg ctgatgtgaa cggagtgtcg gtcatactac aggtttgccg
     4201 gtttatacat aataactgaa cagggcatta tattaactca gggggagtag atacgatgga
     4261 ttatagaaag gaatcactca ggctgcataa tgaatggaag ggtaagatcg aagtcataag
     4321 cactgtacct gtaacgaaca aacaggagtt gtcgctggct tatacgcccg gtgtggcgga
     4381 accctgcctc gcgatccaga aggacgtgga cctgtcatat gaactgacga gaagatggaa
     4441 tctcgttgca gtcgtgaccg acggcaccgc cgtacttgga ttgggggata taggccctga
     4501 agccgggatg cctgtcatgg aaggaaagtg cgtattgttc aagtcgttcg cggatgttga
     4561 cgcgttcccg ctttgtatac gttcgaagga cgtcgatgac atagtgaaga ctataaaact
     4621 gatctcaggc agtttcggcg gcatcaacct ggaggacata tccgctccga gatgcttcga
     4681 gatagaaagg cgcctcaagg cggaatgcga tatcccgatc ttccacgacg accagcacgg
     4741 aaccgcggtt gtcactctcg ctgcaatgat aaatgcactt aagatcgtta agaaggatat
     4801 cagggatatc gaagtcgtaa tcaacggatc gggagcggca ggcattgccg taacaaggct
     4861 gctgatggca atgggtctga agaaggtcat cctttgcgat acaaaaggcg ccatatatga
     4921 gggcagggaa aacctgaacg cggaaaaggc cgagatggcg aagatttcga atttcgaaaa
     4981 gaagaaagga acgctgaagg atgtcatcgt cggcgccgat gtgttcatcg gcctgtctgt
     5041 ggcgggtg
//
LOCUS       DAOSCC010000180         5037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10467_length_5037_cov_4.024689, whole genome shotgun sequence.
ACCESSION   DAOSCC010000180 DAOSCC010000000
VERSION     DAOSCC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10467_length_5037_cov_4.024689"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLG72_09125"
     CDS             complement(<1..730)
                     /locus_tag="PLG72_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ80994.1"
                     /translation="MKNIIRLVKYAKPYWHLLVISALSIIAITVLNLVGPRLITWLID
                     ILTSGFSSDSLGDVRTIALALTATYILKILFRYLNSYLSHKAAWNLVADMRVRVYDHL
                     QKLSLRFYQDKQTGQLMSRTTNDTATFEMLIAHVVPELFGNVLVVIGVAVVLFLTNVQ
                     LAMLTLIPIPFLLFGSWVFANKVRPNFRAAQGNLAELNATLQDNISGMKEIQVFNQQR
                     KEKDRVRAKAGSYAQAILHALKLSA"
     gene            complement(953..3394)
                     /locus_tag="PLG72_09130"
     CDS             complement(953..3394)
                     /locus_tag="PLG72_09130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOJ80995.1"
                     /translation="MNLTENAIKVLEKRYLAKDETGKLLEDPEGMFKRVAKAIAAADK
                     PYATSSELKKIEKEFFSMMANLEFLPNSPTLMNAGRPLGQLSACFVLPIEDTMEGIFD
                     SIKNAALIHKSGGGTGFSFSRLRPKGATVNSTGGVASGPISFMKVFNAATEAVKQGGT
                     RRGANMGILSVDHPDIREFITCKQDNRDITNFNISVALTEEFMKAAEQGLEYDLIDPR
                     TNKSVKKENAREIFNMIVDNAWNNGEPGIIFLDRINKDNVTPELGEIESTNPCGEQPL
                     LPYEACNLGSINLSLMVKESDGKFEVDFDRLRNIVRKAVHFLDNVIDVNRYPLPQIDK
                     MTRGTRKIGLGVMGWADMLCKLGIPYNSQQAIDLAEKVMHFIQDESRRMSVELAEKKG
                     VFPYFDKSIYKELGARVRNATTTTIAPTGTLSIIAGCSSGIEPLFAISYIRNVMDNDE
                     LIEVNPIFRKVAEEEGFYSDDLMRQIAKKGSIHDFAEIPPHIKEVFVTAHDISPEWHV
                     RMQASFQKYTDNAVSKTVNLSHDATRDDVRAVFELAYKTYCKGVTIYRDGSREMQVLN
                     IGQVKGKEGTKTEAGASEARAEVVPQQAMPVVAPRPRPDITTGFTEKVKIGCGNLYIT
                     VNYDEHGICEVFTNTGRAGGCPSQSEATARLVSVALRSGIDAKSIVEQLKGIRCPSTI
                     RQKDLKVLSCPDAIGRLIEKVLKLQNGNGNGNGNGNGKENGFSNGDAKIIQDVVKKSM
                     TDTGRIDYIPRPTGNSPEYDERRLSSMAEINENDSGYSLSETLHMTHCPDCGNPVEHE
                     GGCVICRHCGFSKCG"
     gene            complement(3649..4950)
                     /locus_tag="PLG72_09135"
     CDS             complement(3649..4950)
                     /locus_tag="PLG72_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOJ80996.1"
                     /translation="MKFRKPARLVSMLLVLMILVTGCMGQKLQQNTGDAGNTAGAEGN
                     ETAGETENGDAAASEQGSETADPEEQAEGNDPNQGEAEGSENAEGTEPGEKEEERIKV
                     KALYLTGWTAGSSQKLEHYVELANTTEINAYVVDIKDDDGYVGYESQVPEVRENGTWK
                     KKYDAEKVLKAFHDNGIYVIGRLVVFKDPVYSLKRPDLAVKHVNGGLWKDKDGITWLN
                     PYNRETWDYTISIAKEAVALGFDEIQFDYVRFPDGTKKTGDFSGIDKEKYEAINEFLA
                     YAKQQITEVPISADVFGIILESPKDTENIGQYLELVGMDIDYISPMVYPSHYALGQIV
                     NKVKFDVPDLKPYEVVYNSLVKCRDRISQVEGYKAKVRPYLQDFTATWLKDRKTGKKY
                     YQEYGPEQVRQQIQAVYDAGYEEWILWDARNTYSEEALLKE"
BASE COUNT         1009 a         1364 c         1171 g         1493 t
ORIGIN      
        1 cagcgctcag tttcagtgcg tggagtatcg cctgggcata gctgccggct tttgctctca
       61 cgcggtcctt ttccttacgc tgctggttga aaacctgtat ctctttcatt cctgaaatgt
      121 tgtcctgcag ggtggcattg agttcggcaa ggttcccctg ggcagcgcgg aaattgggcc
      181 tcaccttgtt tgcgaagacc cagcttccga aaagcaggaa cggtatcggg atcagcgtca
      241 gcatggctaa ctgtacattg gtgagaaaca gaacaactgc cacgcctatg acgacaagga
      301 cattcccgaa cagttcgggt accacatggg cgatgagcat ctcgaatgtg gccgtatcgt
      361 tggtggtcct ggacatgagc tgtccggtct gcttatcctg gtagaacctc agcgacagct
      421 tctggagatg gtcatacacc ctgaccctca tgtcagcgac aagattccac gcggctttgt
      481 ggctgagata gctgttcagg tacctgaaaa gtatcttcag tatgtacgtc gccgtcaatg
      541 ccagcgctat cgtcctgaca tctccaagcg aatcactgct gaatccgctg gtgagtatat
      601 ctatcagcca tgtgatcagc ctcgggccga ccaggttgag cacggtaatg gcgatgatgc
      661 tgagagccga tatcacaagc aggtgccagt atggttttgc atatttgaca agtctgatta
      721 tgtttttcat attacctctc catttttgta ctttgcattt tttattgttg tatcctggtt
      781 cgaaaattac tgttatgaat attataacga tgaatgtcat aatgcgccaa agaatttttc
      841 gattgcatgg attttccttg cctcaaaaaa ataaggtgaa gcttcttcac cttatttttg
      901 gagagatttg agattatgca gtttctggca tgagtcacac ggccgggacc cgtcagccgc
      961 atttgctgaa cccgcaatgc ctgcagatca cgcagccgcc ttcatgttcc accgggttgc
     1021 cgcagtcggg gcagtgtgtc atatgcaacg tctcactcag gctgtacccg ctgtcgtttt
     1081 cattgatttc agccatgctg ctcagacgtc tttcatcata ttcgggagaa ttgcctgtcg
     1141 gcctcggtat atagtcgatc cttccggtat ctgtcatgga ctttttgaca acgtcctgta
     1201 ttatctttgc atcaccgttg ctgaagccgt tctctttgcc gttgccgttc ccattcccat
     1261 tcccgttgcc gttctggagc ttaagcacct tttctatcag ccggccgatc gcatcagggc
     1321 aggacaatac cttcagatcc ttctgcctta tagtcgacgg acacctgatg cctttaagct
     1381 gctccacgat ggatttcgca tcgatgcctg acctgagcgc tacagacacg agtcttgccg
     1441 tagcttcgga ctgagacgga catccgcctg cacgcccggt atttgtaaac acttcgcaaa
     1501 tgccgtgttc gtcatagtta acagttatat agaggttccc gcagccgatc tttacctttt
     1561 cagtgaaccc ggtggtgata tcgggacgcg gtctcggcgc gaccacaggc atcgcttgct
     1621 gcggtacgac ttcagccctg gcttcggatg ccccggcttc ggttttcgta ccttccttcc
     1681 ctttgacctg gccgatgttc agtacctgca tctcccggct gccgtccctg taaatggtca
     1741 cgcccttgca gtatgttttg tatgccagtt cgaacaccgc cctgacatcg tcgcgggtgg
     1801 cgtcgtggct gaggtttaca gtcttggaca cggcattgtc ggtatacttc tggaacgacg
     1861 cctgcatcct cacatgccac tcgggagata tatcatgggc tgtgacaaac acttccttta
     1921 tatgtggcgg gatctccgca aagtcatgga tcgatccttt cttagctatc tgtctcatga
     1981 gatcgtcaga atagaagcct tcctcttcag cgactttcct gaagatcggg ttgacctcga
     2041 tcagttcgtc attgtccatt acgttcctga tgtacgatat tgcaaataac ggttcgatgc
     2101 cgctggagca gccggcgatt atgctcaaag tccctgtggg agctatagtt gtggtcgttg
     2161 cattgcgcac ccttgcgccg agttccttgt aaatgctctt gtcgaagtac gggaacacac
     2221 cttttttctc cgccagttca acggacatcc tgcgggattc atcctggatg aagtgcatga
     2281 ccttctcggc aagatcgatc gcctgctggg aattgtacgg tatgccgagc ttgcagagca
     2341 tatccgccca gcccatgacg ccaaggccga tcttccttgt cccccttgtc atcttgtcta
     2401 tctggggcag aggatagcgg ttcacatcta tgacattgtc aaggaaatgc acggcttttc
     2461 tcactatgtt cctcagcctg tcaaaatcga cctcgaactt gccgtcgctc tccttcacca
     2521 taaggctcag atttatggag ccgaggttgc aggcctcata cggaagaagg ggctgctcac
     2581 cgcacgggtt ggtgctctct atctcgccca gttcgggagt cacgttgtcc ttgttgatcc
     2641 tgtcgaggaa tatgatgccg ggttcgccgt tgttccacgc attgtcgacg atcatgttga
     2701 atatttcacg ggcgttttcc tttttgaccg acttatttgt cctcgggtct atcagatcat
     2761 actccaggcc ctgttccgcc gctttcatga actcttccgt cagcgcgacg cttatattga
     2821 aattggtgat gtccctgtta tcctgcttgc atgtgatgaa ttcgcgtatg tccgggtggt
     2881 ccacactgag tatgcccatg ttggcccctc ttctcgtacc gccttgcttc accgcttcag
     2941 ttgccgcatt gaacaccttc atgaaactga tagggccgct ggccacaccg cctgtggaat
     3001 tgaccgtcgc gccctttggc ctgagccttg aaaagctgaa tcctgtgccg cccccgcttt
     3061 tatgtatcag tgccgcattc ttgatagaat cgaatatgcc ttccatcgta tcctcgatgg
     3121 gtagcacaaa gcaggcgctc aattgtccaa gaggcctccc cgcgttcatc agtgtcggcg
     3181 aatttggcag gaactccaaa tttgccatca tgctgaaaaa ttctttttcg attttcttca
     3241 attctgagga agttgcataa ggcttgtctg ccgccgctat cgctttggct acgcgtttga
     3301 acatcccttc ggggtcttca agaagtttac cagtttcgtc cttagcaaga tagcgtttct
     3361 caaggacctt gatggcattc tcagttaaat tcatggctcg gcctccttga acgtgacaca
     3421 atattttgtg gaatcgtatt tttcctggca ctgtatattg tatcactttc gcagatttgt
     3481 cgtcaagatg aaaatgaggc aacaggcacc aaacagggaa tggttctcct gcctggacat
     3541 ggcgccgcga cagccggaga aaaatcgtgc caggtacgaa acacggacac atatttctga
     3601 ttttaatatg tgtctgtatg tcgtgcctgg cacaatatcc aggtttcttt actccttcag
     3661 caatgcctct tcactgtagg tgttccttgc atcccacagt atccactctt cgtagcccgc
     3721 gtcatatacg gcctggatct gctggcgtac ctgttcgggg ccgtattcct ggtaatactt
     3781 cttcccggtt tttctatctt tgagccatgt tgctgtgaaa tcctgcagat agggccttac
     3841 ctttgccttg tagccttcca cctgagaaat cctgtcccgg catttcacaa gtgaattgta
     3901 gaccacttca tagggtttga gatcgggcac atcgaacttc accttgttga ctatctgtcc
     3961 gagagcatag tgggacggat agaccatcgg cgatatgtag tcgatgtcca tgcctacgag
     4021 ttccaggtac tggcctatat tctccgtatc cttcggactt tccagaatga tgccgaacac
     4081 gtcagctgaa atgggcactt ctgttatctg ttgctttgca tatgccagga actcgtttat
     4141 agcctcatat ttttctttat ctatgcctga gaaatccccg gtctttttgg tgccgtcggg
     4201 gaacctgaca taatcgaact gtatttcgtc aaatcccagg gcaacggctt ctttagctat
     4261 gctgattgta taatcccacg tttccctgtt gtacgggtta agccatgtta tgccgtcctt
     4321 gtccttccac agcccgccgt tcacatgctt gacggccaga tcgggcctct tcagggaata
     4381 gaccggatcc ttgaacacga ctaatctgcc gatcacatat atgccattgt cgtggaacgc
     4441 tttcaatact ttttcggcgt catatttctt tttccatgtg ccgttttcac ggacctcagg
     4501 cacctgcgat tcatagccga catatccgtc gtcatccttt atgtctacta cgtaagcatt
     4561 gatctcggtc gtgtttgcca gttccacgta atgctccagt ttctgcgaac tgcccgccgt
     4621 ccagccggtc aaatagagcg ccttgacttt tatacgttct tcttcttttt ctcccggttc
     4681 ggtaccctcc gcattttcag agccttcagc ctcaccctgg ttcggatcgt ttccctccgc
     4741 ctgttcttcc ggatcagcgg tttccgatcc ctgctccgaa gctgctgcat cgccgttttc
     4801 cgtttcgccg gcagtttcat tgccttccgc tcctgccgta tttccggcat ctcctgtatt
     4861 ctgctgcagt ttttgcccca tgcatcctgt gaccagtatc atcaggacca acaacatcga
     4921 gaccagcctg gccggtttcc tgaatttcat gattatgccc cccatattat cctttgtgca
     4981 gccttcagta ttttatgtac acttgcaaat tttattatag gtatttatta gacatga
//
LOCUS       DAOSCC010000181         5032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10484_length_5032_cov_5.238497, whole genome shotgun sequence.
ACCESSION   DAOSCC010000181 DAOSCC010000000
VERSION     DAOSCC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10484_length_5032_cov_5.238497"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1937
                     /locus_tag="PLG72_09140"
     CDS             135..1937
                     /locus_tag="PLG72_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80997.1"
                     /translation="MFRKCLAVIICMAVFVSLVGCEGVKSLFSQKDTGGPDGMIENAD
                     EQTDIADPDAARENGMDAGIPESEYLLFMRINDNSTKESYDVLELTEDTITYSFYDYS
                     DPGNPVLKEKTVPFDEILAKENGRDKYFITIYLFDGVNEYELDLTRDLEEKIREYPEF
                     RETLGTVLGKPFAKPPQDNVITSEDINATAMRNAGVSEKAGNGTGAAKDTGSGENTEV
                     QQPTGSVIGYDVYGELYADNDGTAGYRIEYPQISGLGNESVRKKINDTIRAEALNALA
                     YYKDSRPSADVDISYRIRRSSPCLLSVEYRETGKNTDEPQAGGPCFTVNIDIRTGNRI
                     RLKDMVNVIDGFAIKLFDDRSRAVGGHGTEPLADLGFLKLKELLAEADITKNVGSPEE
                     PHVFSYLTTDSLGISIYFGQGDGVLPAGRQKTGGSATGGRAVYEIGFGDLEEDMIIDE
                     TLRGELLSTGVEQVASLDGVEFEGLGQAKLTVGKYCENGVMRLKLLLEERNSGLVYVF
                     PEPTDIPWYFLDLSALEVLDADADGLSDIVAIVDYITGVGTDGAVPFPYAHIYFRRPD
                     GFVRDPELEQEINEEEFSSAEDVARLADKYSGRR"
     gene            2111..2380
                     /locus_tag="PLG72_09145"
     CDS             2111..2380
                     /locus_tag="PLG72_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ80998.1"
                     /translation="MRQRDTALFIVNCLLILSVIAILGSSLFIGNSVGLSDNGDFNRV
                     ITPNRIYYEDGAREPFVFTDRFKLAFEGDGRFEKIFNTLFTLDGR"
     gene            2756..4411
                     /locus_tag="PLG72_09150"
     CDS             2756..4411
                     /locus_tag="PLG72_09150"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOJ80999.1"
                     /translation="MGSKFANIPIGLLFALAGLAFLGFRDGGRGFKAVTAGGLIVSLA
                     AAALFYIAVPNWMDKATTYQAVFFGILKDSPDPEKDLEQLDLPLYMADLQNTNYYMDG
                     HKVDISSDDFQRDFYRKVSKADVLKFYLRNPARFLDKLDISCKNSSLIRPKYLGNLGP
                     GNERLTLVNEFGLWSGIRAKMPFDSLFFTFVLFLVSLIIIITELRSAFRAKPKDPKKI
                     AVCAYYTALLGGTAINLAVPVVANGEADIAKHMFGFINGMDLIFFSVMFWIFYKLAAI
                     CRSCIGRIADPVKIIASRNRTVPVLVSVLAVIAVIAAAAAFFAGYGKTPGSSKKGAFV
                     TIGRLDGTPLLWRILDEDESGILLLADAPVAYMPFDTPSGDGDANRQKYGSNLWRDSS
                     LREWLNGDFTAKCFSESEQELIQDYKNKVILARHDLDMAVSGSNDFFWTHVPTMVDFG
                     YDEAYSDFVADRIFLLDVRQFREYLAEGNIAYARKYPWWLETPYYLNSSMVRVVDTDG
                     YVYMKDALVDHIAVLPAMYIKPEVSVSDGDGSREDPFIFELKR"
     gene            4534..>5032
                     /locus_tag="PLG72_09155"
     CDS             4534..>5032
                     /locus_tag="PLG72_09155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ81000.1"
                     /translation="MNSDRTVYSVIVPLYNEEAVVQDSYKRIREVMDTTGESYEIIFV
                     NDGSRDSTYEKVREILKNDKNVRLISFSRNFGHQSAITAGMDHCTGQAVIVIDADLQD
                     PPEVMLKMIEKWKEGYEVVYGKRIKRKGETLFKKATAKIYYKILSKTTDVEIPIDTGD
                     FRLIDR"
BASE COUNT         1343 a         1083 c         1336 g         1270 t
ORIGIN      
        1 agcattattg ttaaaacatg aattatatgt tatcattttt tcagaaatcc cacatttaag
       61 agcgctgtgc aatcatttcc gctgagttgc cttcatggcg catgcgtgat tcggcacagg
      121 gacaggggaa aataatgttt aggaaatgtc ttgctgttat catatgcatg gctgtattcg
      181 tatcccttgt gggctgcgaa ggagttaagt ctcttttttc acaaaaagat accggcggtc
      241 cggacggtat gatcgaaaat gccgatgagc agacggatat cgcggatccc gatgctgccc
      301 gcgaaaacgg aatggatgca ggtatcccgg agagcgagta tcttctgttt atgaggataa
      361 acgataacag cacgaaagaa agctatgatg tgctggaact cacggaagac accatcacct
      421 acagtttcta tgattacagc gatcccggga atcctgtcct gaaagaaaaa acggttccct
      481 ttgacgagat acttgcaaaa gagaacggca gggacaaata tttcatcacc atatacttgt
      541 tcgacggagt caatgaatat gaactggacc ttaccaggga ccttgaggag aagatcaggg
      601 aatacccgga attccgcgaa acacttggga ccgtgcttgg gaagcctttt gcaaagcccc
      661 cgcaggacaa tgttataacc agcgaagata tcaatgcaac ggcaatgaga aatgccggcg
      721 tatctgaaaa agccggcaat gggaccgggg ccgccaaaga taccggcagc ggtgaaaata
      781 ctgaagtaca gcaaccgaca ggatctgtaa tcggatatga tgtatacggg gaactctatg
      841 cggataatga cgggacggcg ggatacagga tcgagtaccc ccagatatcg ggacttggca
      901 atgaaagtgt ccggaagaag ataaacgata cgatacgggc tgaggcgctg aatgcgctcg
      961 catattacaa agattccagg ccatcggcag atgtagatat aagttaccgt atcaggagga
     1021 gttcgccttg cctgctaagc gtagaatacc gtgagacagg gaaaaacact gatgaaccgc
     1081 aggccggcgg tccttgtttt acagtaaaca tcgacatcag gacagggaac aggatcaggc
     1141 tgaaggacat ggtaaatgtc attgacggtt tcgccatcaa gcttttcgac gacaggtcca
     1201 gggcagtcgg cggccatggg acggaaccgc ttgcggacct tggcttctta aagctgaaag
     1261 agcttcttgc tgaagcggat atcacgaaaa acgttggctc gccggaagaa cctcatgttt
     1321 tcagttatct tacgacggat tcgctgggca tatcgatata ttttggccag ggcgacggcg
     1381 tgctgcctgc cggaaggcag aagacaggcg gctcggcaac tggcggtcgt gcggtatatg
     1441 agatcggatt cggggatttg gaagaagaca tgataattga cgagaccctt cggggagagc
     1501 tcctgagtac aggcgttgaa caggttgcgt cccttgacgg tgtggaattt gaaggcctcg
     1561 gacaggcgaa gctgacagtc gggaaatatt gcgaaaacgg cgtgatgaga ctgaaactgc
     1621 tgcttgagga aagaaacagc ggccttgtat atgtgtttcc cgagcccacg gatataccat
     1681 ggtatttcct tgacctgtcg gctttggaag tgctggatgc cgacgctgac ggactcagtg
     1741 acatcgttgc catcgtggat tatatcacag gtgtgggaac cgacggggct gttccgttcc
     1801 cctatgccca tatatatttc aggcggcctg atggattcgt ccgtgatccg gagctggagc
     1861 aggagataaa tgaagaagag ttttcgtcgg cagaagatgt ggccaggctg gcggataaat
     1921 atagcggcag gagataagag accatctcat ttttacatcg gcctaacatg tgatctccgc
     1981 cgccgggtaa acgctttgta atgttgcgat ccttgtgcct ccctgttttt agtggtataa
     2041 tctcatacat gtcataggac tgctccctga tatatgaacc aaaaaggact gttcggaggt
     2101 tgtagcgtaa atgaggcaaa gagatacggc tctttttatt gtaaattgtt tgctgatttt
     2161 atccgttatc gcaatactgg gcagttcttt gttcatcgga aacagcgtag gcctttcgga
     2221 caatggagat ttcaaccgcg tgatcacgcc caaccggatt tattatgaag acggcgcaag
     2281 agagccgttc gtgttcaccg atcgctttaa acttgccttc gaaggagacg gcaggttcga
     2341 aaaaatattc aacacacttt ttacattgga tggcagataa atcaccaccc agcatttgtt
     2401 catcaagatc tcgatattgc ttaatctctg cttcaatgcc gttagcggga ccgatttcag
     2461 cgtatatcat atagaatggc tgggtgctgc ctactgcctg ttccttgttg ttgcggtgtc
     2521 actgttcatt tttacagttc gcacgggaaa aacgtggctt gatatcattt ttaaacttct
     2581 gttcatattc tttttttgcg acattgccta cacagcctat ttcaattcct tctacggaga
     2641 agccttgcag tttgtttcct tcctgttcat attcgcatgt atggctgccg tgattttttc
     2701 aaagaaacac ggaaaactgt tctgcctgct ttatttttca ggagtcatcc tttttatggg
     2761 ttccaagttt gcaaacatac cgatcggctt gctgtttgcc cttgcggggc ttgctttttt
     2821 gggtttcagg gatggcggca gaggatttaa ggctgtgacg gcgggcggac tgatcgtttc
     2881 cctggctgca gcagcactgt tctatatagc ggtacccaac tggatggata aggctaccac
     2941 ataccaggct gtatttttcg ggatactgaa ggattcgccg gatcctgaaa aggacctgga
     3001 gcagctggac ctgcctttgt atatggcgga cctgcaaaat accaattatt acatggacgg
     3061 ccacaaggta gacatcagtt ctgacgattt ccagagggat ttctaccgga aggtctcaaa
     3121 agcggacgtc cttaaattct atctgcgcaa tcctgcaaga ttcctggaca agctggatat
     3181 ttcctgcaaa aacagttcgc tgatcaggcc caagtacctg gggaacctgg ggcctggcaa
     3241 tgaaaggctt acacttgtca atgaattcgg cctttggagc ggtatcaggg ccaaaatgcc
     3301 ctttgacagt ctttttttca cctttgtatt gttccttgtc agcctcatta tcattattac
     3361 ggaactgaga tccgcattcc gtgcgaaacc gaaggatccg aaaaagatcg ccgtctgcgc
     3421 atattataca gcgcttcttg gaggtactgc cataaacctg gccgttcctg tggttgcaaa
     3481 cggagaagcg gatattgcaa agcacatgtt cggttttata aacggtatgg accttatatt
     3541 tttctccgtc atgttctgga ttttctataa actggcggcg atctgcagat cctgcatcgg
     3601 aaggatagct gatcccgtaa aaattatagc atcgcgaaat cgcacagttc cggtccttgt
     3661 atccgtcctt gcagtcattg ctgtgatcgc agctgcagca gcattttttg ccgggtacgg
     3721 gaaaacgcca ggaagttcca aaaaaggtgc ttttgtaact ataggccgcc ttgacgggac
     3781 accgctgctg tggaggatac tggatgagga tgaatcaggc atcctgctgc ttgcggatgc
     3841 acctgtggca tacatgccgt ttgatactcc ttcaggagac ggagatgcga acaggcaaaa
     3901 atacgggagc aacctgtggc gggacagcag cctgagagag tggctcaatg gagattttac
     3961 tgcaaaatgc ttcagcgaaa gcgaacagga actgatacag gattataaaa ataaagtgat
     4021 cctggccagg catgacctgg acatggctgt cagcggcagc aatgactttt tctggaccca
     4081 tgttccgacc atggtcgact ttggctatga tgaagcatat tccgacttcg ttgccgacag
     4141 gatctttttg ctggatgtcc ggcagttcag ggaatatctt gcggaaggaa atatcgcgta
     4201 cgccaggaag tatccctggt ggcttgagac gccatactat ctgaattcaa gcatggtaag
     4261 ggtcgtagac accgacggct atgtatacat gaaagacgcg ctcgtggacc atatcgctgt
     4321 attgccggcc atgtacataa agccggaagt gtcggtttcg gacggcgacg gcagccggga
     4381 agatcccttt atctttgaat tgaaaaggtg aattcaaaag ttgagatata taaaagaaaa
     4441 agcgcgtggt tatatgatta tccgtaaagg ttattgtcga tataatttac ggaaaggtga
     4501 aggaatgctg tgatgatccg ggaggcggca ctcatgaaca gtgatcggac ggtttattct
     4561 gttattgtac ccctgtacaa tgaagaagcg gtggttcagg attcatataa gcgtatcagg
     4621 gaagtcatgg acactacggg agaatcctac gaaatcatat ttgtcaatga tggaagcagg
     4681 gacagcacgt atgaaaaggt ccgtgagatc ctgaaaaacg ataagaatgt gcggctcatc
     4741 agtttctcca ggaattttgg ccatcagtcg gccattactg ccggaatgga ccactgtacg
     4801 ggccaggccg tgatcgttat cgacgccgat ttgcaggacc ccccggaagt catgctgaaa
     4861 atgatcgaga aatggaagga aggctatgaa gtcgtatatg gcaaaaggat caaaagaaag
     4921 ggagaaaccc tgtttaagaa agcaacggca aaaatatatt acaagatcct gagcaaaacg
     4981 accgacgtag aaatcccgat cgatacggga gatttcaggc ttatcgacag ga
//
LOCUS       DAOSCC010000182         5027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10498_length_5027_cov_3.893403, whole genome shotgun sequence.
ACCESSION   DAOSCC010000182 DAOSCC010000000
VERSION     DAOSCC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10498_length_5027_cov_3.893403"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLG72_09160"
     CDS             complement(<1..384)
                     /locus_tag="PLG72_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ81001.1"
                     /translation="MTDGVVTGSMTDRVKADGNGSVEKKTVEKKFVDFESALSLTGEE
                     VDRALLSSPAVVRTYTSHLALARGKFFRAVSLLTCAMNSDSLIDENAVKLAAAVEVLH
                     LATLVHDDVIDDADIRRGLPTLQKKF"
     gene            complement(377..2497)
                     /locus_tag="PLG72_09165"
     CDS             complement(377..2497)
                     /locus_tag="PLG72_09165"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ81002.1"
                     /translation="MDTKIVIVGAGYAGILTAKKLAKRFRKDKNVSITVIDKNPFHTM
                     LTELHEVAAGRVDEDEIRISLKRVFAGRKVDVKQDLVTDIDFKKRTVVGTNERYEYDY
                     LVLCAVSKPTFFGVSGAREHAFTLWSYEDAIVLKDRIPDMFRRAANETDEGEKKRLLT
                     FHIVGAGFTGVEMAGELAEYVPVLCDKFEVDRELVSISVIDVLERTVPTLPPKLSAKV
                     ERRLKKMGVRVLLNTRVLSIGEDYIEIGDAEKPIHEKAGTVIWAAGIESADITGKAAE
                     DLESAGRGRIKVDRYLRSVNDERVYVIGDNMFYIPPGEEHPVPQVVENCEHSAAVAAH
                     NIVCAVTGKDGMIEYRPGFHGIMVSIGGRYGVAYVGTAKKKISLASFFAMFVKHFINM
                     VYFVQLLGWNKVFSYMKKEFFTIRHCRSFVGGHFSNRTPSFLLVPLRIWLGAVWVFEG
                     VKKILEGWFVSPKLAGFFNGANQWYNSILSGAGGAADGTSGATPAAADAVSQATGAVA
                     DAVSSATGAVADALSSAASAVADAVSSATAVAADAASTATPAAEAVTSIGKAIINFDF
                     LGLFKVLFVSGKDPTDAALSDYAFKLDVPLINRMINDLILPSEGFTMFMQIAIVVAEI
                     LIGLALIGGLFTTPASAASLVLQAMFVTTTGLYLTTFWMVFAGIAVLVGGGRIFGLDY
                     YVMPALKEKWKNVKWVRKWYLYND"
     gene            complement(2712..4100)
                     /locus_tag="PLG72_09170"
     CDS             complement(2712..4100)
                     /locus_tag="PLG72_09170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOJ81003.1"
                     /translation="MLAEKSMEKIVALCKNRGFVYPGSEIYGGLANAWDYGPLGVEFK
                     NNVKKAWWKKFVQENTYNVGVDCAILMNRRVWEASGHVGGFSDPLIDCKSCKTRHRAD
                     KLIEDWNAENGIELVVSGWKNEQLMEYIKEKGVKCPNCGSSNFTDIRKFNLMFKTYQG
                     VTEDSQAEIFLRPETAQGIFVNFKNVQRTTRRKMPFGICQIGKSFRNEITPGNFTFRT
                     REFEQMELEFFCKPGTDLEWFAYWKDFCYQWLLNYGISKENLRLRDHSKEELSHYSNA
                     TTDIEYKFPFGWGELWGIADRTDFDLRQHMEYSKEDLSYFDPATNEKYIPYCIEPSVG
                     ADRVALAYLCEAYDEEELEEGDVRTVLHLHPALAPVKIAVLPLSKKLGEDAFKIYEKL
                     VKKYVCEYDETGSIGKRYRRQDEIGTPYCVTFDFDSLEDASVTIRDRDTMDQVRIKID
                     ELENYFEGKFDF"
     gene            complement(4328..>5027)
                     /gene="coaBC"
                     /locus_tag="PLG72_09175"
     CDS             complement(4328..>5027)
                     /gene="coaBC"
                     /locus_tag="PLG72_09175"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOJ81004.1"
                     /translation="ACSILMPGQDLAGIRMIVTAGPTRESIDPVRYISNHSSGKMGYA
                     VAQAARERGADVLLISGPVGIECPEGVKLVSTATAEEMCKAVLDNFGNCDVVVMAAAV
                     ADYRPAEVSETKIKKNDDEMVLRLVRTPDILKKLGTIKGNRILVGFSAETDHLTDNAM
                     KKLESKNLDMIVANDVTMEGAGFGTDTNIIKIIKRDGTVIDLPLMSKLEAAHRILDEV
                     RGLAAGREHGRGGA"
BASE COUNT         1109 a         1508 c         1077 g         1333 t
ORIGIN      
        1 gaattttttc tgcagtgtcg gaagtccacg cctgatatcc gcatcatcga taacatcatc
       61 atggaccagc gttgcaaggt gcagaacctc gaccgctgcc gccagtttta ccgcattttc
      121 gtctatcagg ctgtcactgt tcatagcgca ggtaagcagt gaaactgccc ggaaaaattt
      181 cccccttgca agcgccaggt ggctcgtata tgtccggact acggcaggcg aagataaaag
      241 cgccctgtcg acctcttccc cggtaagcga aagagcgctt tcaaaatcga caaacttctt
      301 ttcaacggtc ttcttttcaa cagaaccgtt gccgtccgcc ttgacacggt cagtcatgct
      361 ccctgttaca accccatcag tcattataca aataccactt tcttacccat ttcacattct
      421 tccatttttc cttcagagcg ggcatgacat aatagtcaag cccgaatatc ctgcctccgc
      481 ccaccaggac cgcgatgccc gcaaagacca tccagaatgt cgtcaggtat aagcctgtgg
      541 tggtcacgaa catcgcctgc agtacgagtg atgctgccga agccggcgtt gtgaacagac
      601 ctccgatcag ggcaaggccg atcaggatct cagccactac gatggctatc tgcataaaca
      661 tcgtaaagcc ctcgctcgga agtataagat catttatcat acggttgatc aggggcacat
      721 ccagcttgaa cgcatagtcg ctcaaagctg cgtccgtcgg atccttgccg cttacgaaca
      781 gcactttgaa caaaccgagg aaatcgaaat tgattattgc ttttccgatc gaagtcacgg
      841 cttcagccgc cggagtcgca gtacttgccg catctgcagc cactgcagta gccgaactga
      901 ccgcatccgc cactgcgctt gcagctgagc ttaacgcatc agccaccgcc cccgtggccg
      961 aactgaccgc gtcggcaaca gccccggtcg cctggctcac agcatcggcc gctgccggtg
     1021 tggcgccgct cgtcccgtcg gccgctccgc cggcgccgct caatatactg ttgtaccact
     1081 ggttagcgcc attgaagaat cctgcgagtt tcggcgaaac gaaccatccc tccagtatct
     1141 ttttgacgcc ttcaaatacc catacggcgc cgagccatat cctcagcggc accagcagga
     1201 agcttggcgt cctgttcgag aaatgtccgc ccacgaagct cctgcaatgc cttatcgtaa
     1261 agaactcctt cttcatatag ctgaagacct tgttccagcc aagcagctgc acgaaataga
     1321 ccatattgat gaaatgcttg acgaacatcg caaagaacga cgcaaggctg atctttttct
     1381 tcgcggtacc tacatatgca acgccgtagc gtccgccaat gctcaccatt ataccgtgga
     1441 aaccgggcct gtattctatc attccgtctt tgcccgtaac agcgcaaact atattatgtg
     1501 ccgcaacagc ggcgctgtgt tcgcagtttt caaccacctg gggcacggga tgctcctcgc
     1561 ccggcggtat ataaaacata ttgtcgccaa tgacatatac acgctcgtcg ttgaccgagc
     1621 gcaggtatcg gtccaccttt atgcgtcccc tgcccgccga ttccagatcc tcggccgcct
     1681 ttccggtaat atcggcggat tcgatgccgg ccgcccagat gaccgttccg gccttctcat
     1741 gaatcggttt ttcggcatcg ccgatctcga tgtaatcctc gccgatactc aagactcgtg
     1801 tgttcaggag gacgcgcaca cccatcttct taaggcgcct ttcgactttg gccgacagtt
     1861 tcgggggcag cgtcggcacc gtacgttcca gcacgtctat aacggatatc gaaacgagtt
     1921 ccctgtcgac ctcaaacttg tcgcacagca ccggaacata ttcagccagt tcgcccgcca
     1981 tttcaacgcc ggtgaagcct gcgccgacga tatggaacgt gagcagccgc ttcttttccc
     2041 cttcatcggt ttcattggct gctctcctga acatgtcggg gatccggtct ttcagcacga
     2101 tcgcatcttc atagctccag agcgtaaacg catgctccct ggctccgctt acgccaaaaa
     2161 aagtcggttt ggacacggcg cacaatacga gataatcata ctcatagcgc tcattagtac
     2221 cgacaacagt tctctttttg aaatctatat ctgtgacaag atcctgcttc acatcgactt
     2281 tcctgccggc aaaaacacgt ttcaggctga tcctgatctc gtcctcatca acccgcccgg
     2341 ctgcgacttc gtgcagctcc gtaagcatcg tgtggaaagg attcttgtca atgaccgtta
     2401 ttgaaacgtt tttatccttt ctgaacctct tcgccagctt cttcgctgtc agaattccgg
     2461 cataaccggc tccaactata actatcttcg tatccatggc catctcccga attactgtgt
     2521 tattgagatt ccaacagtgt ttttcagcac cgttaaactt ctgacaatat acgtcaccta
     2581 tattacattc ccattattag cattttaaaa cacaactata acaaaaaaag gacagcacct
     2641 gaaagcgccg ctcctgcctg tccctcttct ctacatgtta tgctgggcag ctttcaggga
     2701 ctgtccgtcc gtcaaaagtc gaacttgccc tcgaagtaat tctccagttc gtcgatcttt
     2761 atccttacct ggtccatcgt atccctgtcc cttatcgtta cgctggcgtc ttccagggaa
     2821 tcgaagtcga atgtgacgca gtacggcgtg cctatctcgt cctgcctcct gtaccgtttg
     2881 ccaatgcttc cggtctcatc gtattcgcag acatactttt tgaccagttt ctcatatatc
     2941 ttgaacgcat cttcaccgag ctttttcgaa agaggcagca ccgcgatctt gaccggcgca
     3001 agtgccggat ggaggtgcag tacggtcctc acgtcgcctt cttccaattc ctcttcgtca
     3061 tatgcctcgc acagatatgc gagcgccacc ctgtcggcgc cgacagacgg ttctatgcag
     3121 tatggtatat atttttcatt ggtagccgga tcgaagtaac tcaggtcttc ttttgaatat
     3181 tccatgtgct gcctgaggtc gaaatccgtc ctgtcggcta tgccccacag ctcgccccac
     3241 ccgaacggga acttgtattc tatatccgta gtggcgttgc tgtaatgcga caactcttcc
     3301 ttcgaatggt ccctcagcct gaggttctcc tttgaaatgc cataattaag gagccactgg
     3361 taacagaaat ccttccagta cgcaaaccat tcgagatcag tacccggctt gcagaaaaac
     3421 tcaagctcca tctgctcgaa ctcacgggtc ctgaatgtga aattccccgg agttatctca
     3481 ttcctgaacg acttgcctat ctggcatata ccgaacggca tctttctcct ggtagttctc
     3541 tggacattct tgaaattgac gaaaataccc tgcgcagtct caggcctcag gaatatctct
     3601 gcctgtgagt cctcagtgac gccctggtac gtcttgaaca tcaggttgaa tttgcggata
     3661 tccgtaaaat tgctcgagcc gcagtttgga cacttaactc ccttttcctt tatatattcc
     3721 atcagctgct cattcttcca tccgctcacc acaagttcta tgccattctc cgcattccag
     3781 tcttcaataa gtttgtctgc cctgtgcctt gttttgcagc ttttgcagtc gatgagagga
     3841 tcgctgaagc ctcccacatg tcctgacgcc tcccataccc gccggttcat cagtatggca
     3901 cagtcgactc ccacgttata ggtgttctcc tggacgaact tcttccacca cgcctttttg
     3961 acgttgtttt tgaactctac tccaagaggt ccgtaatccc acgcattggc aagcccgccg
     4021 tatatttcgg aaccgggata gacaaagccc ctgtttttgc ataaagccac gattttctcc
     4081 attgattttt ccgccaacat ctcaagcctc cacgaatatt aatctatttt gaataaattg
     4141 cagtaactca actgttttat ttcagcaaac cagcctcatg tccgctgtct ttgccgccaa
     4201 accaaaacaa ccactatatc ttatggcttt ttatccttaa tacctggttt tatccctggt
     4261 acctggtttc atccctatat gcggtgcgac ttctatctta tatttaataa acttatacta
     4321 aacgtcatca ggctccgcct ctcccatgct cccgtcctgc cgcgaggccc cggacctcgt
     4381 cgagtatcct gtgggcagcc tccagcttgc tcatcagcgg cagatcgatg acagtcccat
     4441 cgcgctttat aatcttgatg atgttcgtat cggtaccaaa gcccgcgcct tccatcgtga
     4501 cgtcatttgc gacgatcatg tccaggttct tcgactccag tttcttcatg gcattatccg
     4561 tcagatggtc ggtttcggcg ctgaaaccga caagtatcct gttgcctttt attgtcccca
     4621 gctttttaag tatatcggga gttcttacga gtctcaatac catttcatcg tcattcttct
     4681 ttattttcgt ctccgatacc tcggcgggcc ggtaatccgc cacggctgcc gccattacca
     4741 ccacgtcgca gttcccgaaa ttatcgagga cagccttgca catctcctcg gcggtggcgg
     4801 tgctgaccag tttgactcct tcagggcatt cgatcccgac aggccctgaa atcaggagga
     4861 catcggcgcc gcgttcacgg gccgcctgtg cgacggcgta acccatcttt cccgatgagt
     4921 ggttcgaaat gtacctgacc ggatctatgc tttccctggt cggaccggcc gtaacgatca
     4981 tccttattcc tgcaagatcc tgtccgggca ttaagatcga acatgcc
//
LOCUS       DAOSCC010000183         5025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10505_length_5025_cov_4.833199, whole genome shotgun sequence.
ACCESSION   DAOSCC010000183 DAOSCC010000000
VERSION     DAOSCC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10505_length_5025_cov_4.833199"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLG72_09180"
     CDS             complement(<1..922)
                     /locus_tag="PLG72_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ81005.1"
                     /translation="MQTISRGSTGPDVRLVQSLLNRIGYNAGPVDGIFGPRTYQAVAA
                     FQANNSLAADGIVGPATWAVLDRLLLGYDTYIVQPGDTLYRIARAYYTTVDAIVTANP
                     GIDPNVIRVGQRLVIPYGIDVVTLDVAPTYEIVRRQIEGLKARYPFLETGSIGKSVMG
                     RELYYVRLGRGSREVSYNASHHANESITTPVLMKFIENYAKAYAARGSIQGYNIRELY
                     DLTSMYIIPLVNPDGVDLVAYWPNYDDPAFTNAARLNRTGLPLPSVWKANIRGVDLNL
                     NYPAEWEKEKQEELERGITGPAPRDYGGEAP"
     gene            1114..2865
                     /locus_tag="PLG72_09185"
     CDS             1114..2865
                     /locus_tag="PLG72_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ81006.1"
                     /translation="MDDTGCMDNTGHNDILRQDSKLLTTLLDVSNLVSSTMELRPLLE
                     AILDKLREIIEYRDAKIFISQGDRASVIAHRSKLRPDQERTYTLPLDREILRNPVVIG
                     DLWSDDELAVTFRNNMREYMDTVFRDARCWMSLPMVYKDTVIGIMTLDHTEPGYYRDY
                     HVELGTAFANQAAIEYENAKLYEEVKRRLQENEQRRRVAEGLKDIMRALNSNRSLEDI
                     LGFIIRQAARLLRADAAALFRLDGDKGLLYTVATYGLPHMENMDSDTGFKAPVLQEAF
                     AGRKPIVRTDPPKTEKSYSGHPVMDAHLKWLRDNCCKMTAFPLICRDEVYGGIALYFK
                     CDGDGTDRDISNEDLTLAMSFADQAALAIDNTRLRKQAEETAVLAERNRLARDLHDAV
                     TQTLFSASIIAEVIPKIWERNRNEGMKRLDELKQLTRGALAEMRTLLFELRPATLVEA
                     PIEDLLRQLGESVTGRARIPVKLDVKTAAKLPPEVKIVFYRVAQEALNNIAKHSGAKN
                     ALVELSVNEDPNLGIIVAALRVTDDGKGFELQNVDSGHFGLGIMKERAESAGAKLTVS
                     SKPGCGTKVELEWVGSK"
     gene            2977..3618
                     /locus_tag="PLG72_09190"
     CDS             2977..3618
                     /locus_tag="PLG72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ81007.1"
                     /translation="MSDEKIRIVVADDHNVVRSGLSAFLLAFDDFELVGEAADGKEAV
                     KLCETLHPDVVLMDLVMPVMDGAQATREIRERFEDIQVIVLTSFKEDELVEGALQAGA
                     IGYLLKNVSADELADAIRKAHAGKPTLAPEATEALIKATGRKKESDISEELTPREKEV
                     LRMMAEGLNNPEIAQKLFVSRSTVKFHVSSILSKLGAASRTEAVSKALQHKII"
     gene            3861..4211
                     /locus_tag="PLG72_09195"
     CDS             3861..4211
                     /locus_tag="PLG72_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ81008.1"
                     /translation="MKVLIADDQADVLNALMVLLEQQEGRFVIEEAEDADSLLSKVEK
                     FRPELLLLDWELSNRDMAEVVPVIKKMAGEVRIIAMSVRPEARDMALYAGADAFVSKG
                     DNSDKLLSVIYTIR"
     gene            4421..4966
                     /locus_tag="PLG72_09200"
     CDS             4421..4966
                     /locus_tag="PLG72_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ81009.1"
                     /translation="MTRTDSELVRSCLMGNNDAFAELITRYKRLIYSVAYKFTKENEE
                     ADDMAQEAFIKIYRSLSKYNDKYKFSTWCVKVATNVCLDHVRRRKLNCVSLEEVENFS
                     ATDTDSPEEHYLRKEKYQVLQDAIDSLPEIYREPIVMYHQKGMSYKEIAEDLGKPMSI
                     IKNRIFRARHTLRENLTTAVS"
BASE COUNT         1300 a         1196 c         1467 g         1062 t
ORIGIN      
        1 gcggcgcctc accgccgtag tctctcgggg cagggcccgt gatgccgcgt tccagttctt
       61 cctgcttttc cttttcccac tcggcgggat aattcaggtt caggtccact ccgcggatat
      121 ttgctttcca tacgctcggc agcggcaggc cggtccggtt cagacgcgct gcattggtga
      181 atgccggatc gtcgtaattg ggccagtatg ctacaagatc gacgccgtcc ggatttacca
      241 gtggtattat gtacatgctg gtcaggtcat acagttcgcg tatgttgtaa ccctgtatgc
      301 tgccgcgtgc ggcataggct ttcgcgtaat tttctatgaa tttcatcagc accggcgtcg
      361 ttatcgattc attcgcatgg tgggacgcgt tgtatgaaac ttctctcgat cccctgccaa
      421 gccttacgta ataaagttcc ctgcccataa cgcttttgcc gatggatcct gtctcaagga
      481 acgggtatct tgctttcagc ccttctatct gcctgcgcac gatctcatat gtaggagcca
      541 catccagggt aacgacatct ataccatagg gtatcacgag ccgctggccg actcttatta
      601 cattcggatc gataccggga tttgcagtca cgatggcatc cacggttgta taataggcgc
      661 gcgctatccg gtataacgtg tctccaggct gaacaatgta tgtgtcatat ccgaggagca
      721 gccggtcaag aacggcccat gtcgcaggtc cgacgatccc gtctgccgcc agcgagttgt
      781 ttgcctggaa tgctgcgacg gcctggtatg tcctcggccc aaatatgccg tccaccggac
      841 cggcattata cccgatcctg ttcagaagac tttgaacgag ccttacatca ggtcctgtcg
      901 acccgcggga gatggtctgc atgaaaaaac accagccttg aatataatgg tcggggtgcg
      961 ccggtgaatg tcactgcgtt ccggacgtca ttgcgaccag attgacacta tcattatatt
     1021 ggggacacag tccaggtgtg ttacggttat tgatatttga aagtatttgg tatacaatca
     1081 aaaaaacatt tttattaccg gccggaggga actatggacg atacaggctg catggacaat
     1141 acaggtcata acgatatact taggcaggat tcaaagctgc tgaccacgct gcttgatgta
     1201 tccaacctcg tctcatcgac gatggaactc aggcccctgc ttgaggctat cctggacaag
     1261 ctgagggaga tcatagaata cagggacgcg aagatattca tatcccaggg ggatcgtgcc
     1321 agcgttattg cccacaggtc caagctcagg cccgaccagg aacggaccta taccctgccc
     1381 ctggacaggg agatactcag gaaccctgtg gtcatcggcg atctgtggtc tgatgacgaa
     1441 cttgcggtca cattcaggaa caacatgcgg gaatacatgg ataccgtatt cagggatgca
     1501 aggtgctgga tgagcctgcc catggtttac aaggataccg tcataggcat catgacgctt
     1561 gatcataccg agccaggcta ttacagggat tatcatgtgg agcttggcac ggctttcgca
     1621 aaccaggccg ctatagagta tgagaatgcc aagctgtatg aagaagttaa gagaaggctg
     1681 caggaaaacg agcagagaag gcgcgtggcc gaaggcctga aggatataat gcgggcgctc
     1741 aactccaaca ggtcgctgga ggacatactc ggctttatca tccggcaggc ggccaggctg
     1801 ctccgcgcgg atgcggcggc gctgttcagg ctcgacggtg ataaagggct cctctatacg
     1861 gtcgcgacat acgggctgcc tcacatggaa aatatggaca gcgatacggg attcaaggcc
     1921 ccggtactcc aggaggcatt tgccgggaga aagccgatag taaggacaga ccccccgaag
     1981 acagagaaat catacagcgg ccatcctgtc atggatgcgc atttgaaatg gctcagggac
     2041 aactgctgca agatgacggc ctttcccctg atatgcaggg atgaggtata cggcggcata
     2101 gcgctttatt tcaagtgcga cggtgatggg acggacaggg acatctcgaa tgaagacctt
     2161 acgcttgcca tgtcattcgc cgaccaggcc gcgctggcga ttgacaacac gaggctcagg
     2221 aaacaggctg aggaaacggc tgttctggcg gagaggaaca ggctggcccg agatctccat
     2281 gacgcggtta cgcagacatt gttctcggca agcatcattg ccgaggtcat accaaagata
     2341 tgggaacgga acaggaacga ggggatgaag aggcttgatg aactcaaaca gctgacccgc
     2401 ggcgcgctgg cggagatgcg tacgctgctt ttcgaactga ggcctgcgac gctcgtggag
     2461 gcgccgatag aagacttact gaggcagctg ggtgaatctg tgaccggcag ggcgcgtatc
     2521 ccggtgaagc ttgatgtaaa gacggctgca aagcttccgc cggaggtgaa gatcgttttt
     2581 taccgtgttg cacaggaagc cctgaacaat atagcaaagc attcgggagc aaaaaacgcg
     2641 ctggtcgaac tcagcgtaaa cgaggatccg aatctcggga tcatcgttgc cgccctccgc
     2701 gttactgacg acggaaaagg attcgagctg caaaacgtgg attccgggca ctttggcctg
     2761 ggcatcatga aagagcgtgc tgagtcggcc ggcgccaaac ttactgtctc gtcaaagccg
     2821 ggatgcggta cgaaggtgga actggaatgg gtaggcagca agtgaaacgg ttcgtgcgga
     2881 agggaaacgg gttgatccgg cgagaaaacg gaaggagcaa aatatcagga agacgtggta
     2941 ttattttcgg tggttggatg aaaggtgggc aatgtgatga gtgacgaaaa aatcaggata
     3001 gttgtcgcag atgaccataa tgtcgtaaga agcggactga gcgcgttcct gcttgcgttc
     3061 gacgattttg aactggtcgg cgaagcggca gacggcaaag aagcggtgaa actgtgtgag
     3121 accctccatc cggacgtagt gctgatggat ctcgtgatgc ccgtgatgga cggcgcacag
     3181 gctacaaggg agatcaggga acgctttgaa gatatacagg tgatcgtgct cacaagcttc
     3241 aaggaggacg aactggtcga aggagcgctg caggcgggag ccataggcta cctgctgaag
     3301 aacgtgtccg cagatgaact tgccgacgcg atacgcaaag cgcatgccgg caagcccaca
     3361 ctggcgccgg aagcgacaga ggcgctgata aaggcgacag ggcgaaaaaa ggagtccgat
     3421 atcagtgagg aacttactcc gagggagaag gaagtgctca ggatgatggc cgagggactg
     3481 aacaatcccg agatcgcgca gaagcttttt gtcagcaggt cgacggtgaa gttccatgtc
     3541 agcagcattt tgtccaagct tggagcagcc agccggacag aagccgtgtc gaaggcgctg
     3601 cagcataaga tcatctagcg gccttttgcc cgccatggag gaaaaacgga agcggcctta
     3661 tatagggcct aaaaacgcag gataaaaata ctgccggaat gaccgccaaa tgcacctggc
     3721 cacctgacca ggcatgtata ctgaccacac ggccagaatt aatctcccca tgtgaatgaa
     3781 gcatggaagg ggatttttta ttacaatcag tattatacat gataaaatag caggcatact
     3841 gcccggaacg aggtgggaac atgaaagtac tgattgccga tgatcaggcc gatgtgctta
     3901 atgcattgat ggtcctgctt gagcagcagg aagggcgttt tgtcatagaa gaggcggagg
     3961 atgcggacag tcttttatcc aaagtggaga aattcaggcc ggagcttctg ctgctggact
     4021 gggaactgtc gaaccgcgat atggccgaag ttgttcccgt gataaaaaag atggccgggg
     4081 aagttcgcat aatcgcgatg agcgtcagac cggaagccag ggatatggct ttgtacgcag
     4141 gtgctgacgc ctttgtgagc aagggagata attccgataa actgctttcc gttatctata
     4201 cgatcagatg acctttcgga tactggcctt ccggccaggc ggaactgatc agatggccgg
     4261 taaaaacaac tgaacacccg gactgcctga aatccgtcct gctggctgat gcggcgcgga
     4321 tcacggataa atataatgaa catagttaaa aaaataacaa cgcgctgtaa tggctgagtc
     4381 ggccagtaac gcgaatctga tcgattgggg gttggatgaa atgacaagaa ctgattccga
     4441 gttggtaaga agctgcctga tgggcaataa tgacgcgttt gcagagctta ttacaaggta
     4501 caagaggctg atctattccg tcgcatacaa gttcacgaag gagaacgagg aagcggacga
     4561 tatggctcag gaagcattca taaagatata caggtcgctt tccaagtaca atgacaagta
     4621 caaattctcc acctggtgcg taaaggtcgc gaccaatgtc tgcctcgacc atgtcaggcg
     4681 caggaaactg aactgcgtct cccttgagga agttgaaaac ttctcggcaa cagatacgga
     4741 ctcgcccgag gaacactatt tgcggaaaga gaagtaccag gtgctgcagg atgccatcga
     4801 cagcctgcct gagatctaca gggagcccat cgtgatgtac caccagaagg gtatgtccta
     4861 caaggagata gctgaggacc tcggcaagcc catgtcgata ataaagaaca ggatcttcag
     4921 ggcaaggcat acactgaggg agaacctgac cacggcagta tcgtgaaagc ggaatgaccg
     4981 tcatgttgtc tgcggtatct ctgtggatca gggtttactg cggaa
//
LOCUS       DAOSCC010000184         4979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10644_length_4979_cov_5.071893, whole genome shotgun sequence.
ACCESSION   DAOSCC010000184 DAOSCC010000000
VERSION     DAOSCC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10644_length_4979_cov_5.071893"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLG72_09205"
     CDS             <1..596
                     /locus_tag="PLG72_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81010.1"
                     /translation="MIVADLPGERGVVESLALARMGYRPVPLYNGVDGRVHSMAVKVG
                     DIRKALFTGADELYGCDISPDAPPVFMLDSNRMAGSGKQPGTYDNRWCVFPQDMPSAS
                     FLLKAGIRKVIVRSDKIRNDLAHILLRYQKEGIEIYLCPDREIKKISVVKPSRFMSLS
                     YRFRVIAGLSRNGAGGFGARIPEPVETSSGGRYYGFG"
     gene            919..2196
                     /locus_tag="PLG72_09210"
     CDS             919..2196
                     /locus_tag="PLG72_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOJ81011.1"
                     /translation="MENSIASSPKFNRWIPVAASIAIQLCMGTAYIWSVFQSYLIISP
                     TTPDALFDWPASYGTLAYSLLLGLLTAGSTIGGKLQEKVSPRTVVIAAGVVLGSGFFL
                     VRFITESSPWLLWLTYGVMGGIGMGMSYTTTIACCQKWFPDKRGLVTGIIVSALGSGG
                     LVFTPVAEALIAKYGVLNTFSILGVVFFIVNVAGAFCIINPPEDYKPRGWTPPAQKAG
                     AAAHSFTPSEAIRTPQFYMVIIAFMCATAAGSMMIPMAKILGLQPDSGLTKEAAVAGV
                     MIISCFNSFGRLFWAGLSDRLGRKRVLLILLVIAALSIIGVSFTRGYLMLAFIAVIGF
                     SYGGFLGVFPALTADFWGTKNVATIYGMILLGFGAGAVVSSYVVAYFSAAKAFTTAFA
                     IAGAAAAAGFVIMALLKAPRLKSGGSSDTTLSI"
     gene            complement(2337..2708)
                     /locus_tag="PLG72_09215"
     CDS             complement(2337..2708)
                     /locus_tag="PLG72_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ81012.1"
                     /translation="MKIYKLMKQKFITVRPDETIEDALARMSSMKVNGAPVVDRNGKL
                     VGMIVKADIYRFLITPGHIEKCPVEWVMSKDVITATVDEEVLTVAKRLRDNNIIAMPV
                     LEGDIVRGVISIEDILDFYIK"
     gene            complement(2938..4557)
                     /locus_tag="PLG72_09220"
     CDS             complement(2938..4557)
                     /locus_tag="PLG72_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81013.1"
                     /translation="MKKAISLLLIMTMLFAAASASVPASYSAAATASEEDPGATAVRD
                     APSYKEEAVYGILDHDGSVKDLYVVNIFNGGAITDYGDYSEVRNLTTSENLVQDGDKI
                     TIDTAADKFYYQGTLKEKELPWDISITYYLDDKQMPADDLAGKSGRLKIAVSVKRNEK
                     ANGIFFHNYALQVALTLDSELCSDIAADDAVIAEAGGKKQITWTVLPGKGADLSVSAN
                     VHDFVMDPITINGIKLVFGIDTDTGMFSDQISQLANAVKELDSGAGELLNGLDQLSDG
                     MKKYMEGLKAFNDGIGQLSAGAVRLDAGAAALKEGLRELAGQNGSLMSGAYAIQQAAF
                     DSANAQLGAIDSRIPALTPENYADVLSKLSQMPGLSDIITGVKNQLDGVVQFTRGLKG
                     YTDGVAQLEAGAGELAKGAGEFSSSASMVAASANELYKAGAELNSAVKELRDGLAAYK
                     GGTKKLRDGTSGIDSEIEEKIDELLAGILGGNEKPVSFVSDKNANVTAVQFALKTGPI
                     NIPKAAKAAAQEPVKLTFWQKLLKLFGLYSE"
     gene            complement(4554..>4979)
                     /locus_tag="PLG72_09225"
     CDS             complement(4554..>4979)
                     /locus_tag="PLG72_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ81014.1"
                     /translation="INLSFPYFAGQPLNFVGYLIISTVQLGATVDYAILFTNKYLDNR
                     KQYPKKDAMRITIGDNLAAILISAVILAASGFTLAYTSNNPIIIELGILLGRGTLLSF
                     IMVACVLPALLVLFDRVIRKTTLNNRFHGIKNSKGSDIE"
BASE COUNT         1069 a         1293 c         1332 g         1285 t
ORIGIN      
        1 ccatgatcgt cgccgatctt cccggcgaga gaggggtcgt tgagagcctg gcgcttgcac
       61 gaatggggta ccggcctgtc ccgctttaca acggggtgga cgggcgtgta cactcgatgg
      121 ccgtcaaggt cggcgacatc aggaaagcac tgttcacagg cgccgacgaa ctttacgggt
      181 gtgacatcag cccggatgct cctcccgtat tcatgctcga ttccaacagg atggccggga
      241 gcgggaaaca gccgggaaca tatgacaacc gctggtgtgt cttcccgcag gatatgcctt
      301 cggcctcgtt cctgctgaaa gcagggatcc ggaaggtcat cgtacgttcc gacaaaatac
      361 ggaacgacct ggcgcatatc ctgctgcgct accagaagga agggatcgaa atctatctgt
      421 gtcccgaccg ggagataaag aaaatttcag tagtgaagcc atcgagattc atgagcctgt
      481 cgtaccgctt cagggtgatc gcgggcctgt caaggaacgg cgccggcggt ttcggcgcga
      541 ggatacctga accggtggaa acaagttccg gcggccggta ttatggattc ggatgagcga
      601 ccgcatccgt gatatgacaa gaccagttga tcgatatatc tgacaggaga agttcaacgc
      661 agacatgggg acggttcccg tgtctgcgga tatgagaggt gacgtgccgg gcacgtcact
      721 tttcatatcc taacgaattt tatcctgttg acaggttatt ttttgcgctt tcttgctttt
      781 gttcaaatcc cgttgctata cggacacggt ttcagttatt atttggtcaa gtggcattat
      841 catactggaa aaaacagcag ttgtaattat ctcaaaaacg ccgggatagt caaaacctca
      901 tatgaaagga tgcgttttat ggaaaacagt attgcatctt ctccgaagtt caaccgctgg
      961 atacctgtcg cggccagtat agccatccag ttatgcatgg gtacggcgta catttggagc
     1021 gtattccagt cttaccttat tatcagtccg acaacgccgg atgccttgtt cgattggcct
     1081 gcatcatacg gaacactggc ttattcactg cttcttgggc tgttgacggc gggaagcacc
     1141 attggaggga aactgcagga aaaagtgtct ccaaggacgg tggttatagc agcaggtgtc
     1201 gtactcgggt ctggattctt cctggtcagg ttcatcacgg aatcctcacc gtggctgctc
     1261 tggctgactt acggcgtaat gggcggtatc ggcatgggaa tgtcttatac gaccacgata
     1321 gcctgctgtc agaaatggtt cccggacaaa agaggccttg tgaccggtat catcgtttcg
     1381 gcccttgggt ccggcggcct tgtgtttacg ccggttgccg aggcgttgat cgcaaaatac
     1441 ggcgtgctga atacattctc catacttgga gtcgtatttt ttatcgtgaa cgtggcagga
     1501 gcgttctgca taatcaatcc tcctgaggat tacaaacccc ggggatggac gccgccggcg
     1561 caaaaagccg gggcggccgc gcatagtttc acaccttccg aagcgatcag gacgcctcaa
     1621 ttttacatgg tgatcattgc attcatgtgt gcgacggccg caggatcgat gatgatacct
     1681 atggcaaaga tactcggact gcagccggac agcggcctta caaaggaagc ggccgttgcc
     1741 ggggtcatga tcatctcctg tttcaattcc ttcgggcgcc ttttttgggc cggcctgtcg
     1801 gataggcttg gcaggaagag agtgctgctg atacttcttg tcattgcggc tctctcgata
     1861 ataggggtct cattcaccag gggatacctg atgctcgcgt tcattgcggt catcggattc
     1921 tcatacggcg gtttcctcgg agtgttcccg gcgctgaccg ccgacttctg ggggacgaaa
     1981 aacgtcgcta cgatctatgg gatgatactt ctcggtttcg gcgcaggcgc ggtggtttca
     2041 tcatacgtgg tcgcatactt cagtgcagca aaagcattca caacggcatt cgctatcgca
     2101 ggggccgcag cagccgcagg atttgtgata atggctctgt tgaaagctcc caggctgaaa
     2161 agcggcggga gttcggatac cacgctcagc atatagacag gaagacgatc cccgcaggca
     2221 aagggacgga tataacagat acggggacgg ttctcatgtt tcaggagtgc cgcttttgag
     2281 acatgggaac cgtccccgtg tatcgaataa ctctctgtcg tgtcaggtac cgccacttac
     2341 tttatataaa aatccaggat gtcttctatg ctgatgacgc cccttacaat gtcgccttcc
     2401 aatacgggca tggcgatgat gttgttgtcc cttagccgtt tggcgacggt gagcacttct
     2461 tcgtccaccg tggctgtgat cacgtctttt gacattaccc attcgaccgg gcatttttcg
     2521 atatggccgg gtgtgatcag aaatctgtaa atgtcggcct tgacgatcat gccgaccagc
     2581 ttcccgttcc tgtcgactac aggcgcgccg tttaccttca tgctgctcat tcttgccagc
     2641 gcatcctcga tagtctcgtc cgggcgtacc gtaatgaatt tttgcttcat gagcttgtat
     2701 attttcattt catcaccacc ctttcctgtt tctgtataaa ttatatcata aaacgacaga
     2761 tggtgcagtg caattcgcaa atgatgagcc ctgcagggct gtatccgcaa tgacggatcc
     2821 ggtcgcatca gaccgttcgg atgctgcggg acgaagacca cagccgctta aaaagatgat
     2881 actccgcccc ggaatagtcc gaagcggagt attgctatgc attacatctt attcgcatca
     2941 ttcgctgtac agcccgaaca gtttcagcag tttctgccag aaggtcagct ttacaggttc
     3001 ctgggccgca gcttttgcgg ctttcggtat atttatcggt cctgttttca gtgcgaactg
     3061 gactgccgta acgttggcgt ttttgtcaga tacgaaggaa acaggttttt cattgccgcc
     3121 aagtatgccg gcaagcagtt cgtcgatttt ttcctctatt tcggaatcga tgccggatgt
     3181 accgtcccgc agctttttcg tgccgccttt atatgcggca aggccgtccc gcagttcctt
     3241 tactgcagaa ttcagctctg ctcctgcttt gtacagttca tttgcagacg ccgcgaccat
     3301 ggacgcggaa gaactgaatt ctccggcgcc ctttgccagt tccccggctc cggcctccaa
     3361 ctgcgccacg ccgtcagtat agcctttcag gccccgcgta aactgcacga ctccgtcaag
     3421 ctggtttttc acgcctgtaa ttatgtccga aagaccgggc atctgcgaaa gctttgaaag
     3481 cacgtctgcg taattctccg gcgtcagtgc cgggatcctg gaatcgatcg cgcctagttg
     3541 ggcgttggcg gaatcgaaag ctgcctgctg tatggcatag gcgccgctca taagcgagcc
     3601 gttctgtcct gcaagttccc gaaggccctc tttcagcgcc gctgctccgg catccagcct
     3661 gacggcgccg gcggaaagct gcccgatgcc gtcattaaag gctttcagac cttccatgta
     3721 ttttttcatt ccgtctgaaa gctgatcgag accgttaagc agttcacctg cgccgctgtc
     3781 gagctccttt accgcatttg ccagctgcga tatctggtcg ctgaacatgc cggtgtcggt
     3841 gtctatcccg aaaaccagtt ttatgccgtt tatagtgatc ggatccatta cgaaatcatg
     3901 cacatttgct gacacagaca ggtcagcgcc tttccccggc aataccgtcc aggtgatctg
     3961 ctttttgccg cctgcttcgg caatgaccgc atcatctgcg gctatatcgg agcaaagctc
     4021 gctgtcgagc gtaagcgcga cctgcagcgc atagttgtgg aaaaaaatac cgttggcttt
     4081 ttcattccgc ttcactgata cggcgatctt cagcctgccg cttttgcctg ccagatcgtc
     4141 tgccggcatt tgtttatcgt ccaggtaata tgttatcgag atgtcccagg gcagttcttt
     4201 ctccttaagg gttccctggt agtagaactt atcagcggct gtatctatgg tgatcttgtc
     4261 gccgtcctgt accagatttt cagaggtggt caggttccgg acctcggagt aatcgccgta
     4321 atcggtgatg gcgccgccgt tgaatatatt tacgacataa aggtccttta cagacccatc
     4381 atggtcaagt atgccataaa ctgcttcttc tttgtaagac ggggcatccc ggactgccgt
     4441 ggccccagga tcctcttccg atgcagttgc agcagccgaa tatgatgccg gaactgaagc
     4501 cgatgccgcg gcaaacagca ttgtcattat cagaagcaga gagatcgctt tcttcattcg
     4561 atatcacttc ctttactgtt ttttatgcca tggaatctgt tgttaagagt cgttttcctg
     4621 atgaccctgt cgaacagtac gagcagcgcg ggcagcacac aggccaccat tataaaggac
     4681 agcagagtgc ctctgccaag gagtatgccc agttcgatta tgatagggtt gtttgaggta
     4741 tatgccagcg taaagccgga agcggcgagg atgaccgccg agatgagtat agccgccagg
     4801 ttgtccccga tcgtgatgcg catggcatcc ttctttgggt attgcttcct gttgtccagg
     4861 tacttattag tgaaaaggat cgcatagtcc acggtcgccc caagctgcac ggtgctgatg
     4921 atcaggtaac cgacgaagtt cagcggttgt cctgcgaaat acgggaacga gagattgat
//
LOCUS       DAOSCC010000185         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10692_length_4961_cov_3.501223, whole genome shotgun sequence.
ACCESSION   DAOSCC010000185 DAOSCC010000000
VERSION     DAOSCC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10692_length_4961_cov_3.501223"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLG72_09230"
     CDS             complement(<1..682)
                     /locus_tag="PLG72_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ81015.1"
                     /translation="MPQSKRDYYEVLGVNRNADEDEIKKAYRKLAKQYHPDMNPGDKD
                     AEAKFKEINEAYEVLSDPQKRARYDQFGHAGVDPNGFGSAGAGFGGFDFGGFSDIFET
                     FESFFGGAGFGRSSRSRTGPQKGADLKYSVEITFEEAAFGVEKEITMSRHENCPKCGG
                     SGSKPGTSPATCKKCGGTGQIQHKHATAFGQFIDVRTCDMCRGEGKIITDPCPECKGM
                     GKVRKTFRK"
     gene            complement(1090..2949)
                     /gene="dnaK"
                     /locus_tag="PLG72_09235"
     CDS             complement(1090..2949)
                     /gene="dnaK"
                     /locus_tag="PLG72_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ81016.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGEPVVIPNPEGSRTTPSVVAFSK
                     TGERLVGQVAKRQAITNPERTVISIKRDMGTDRRVKIDDKSYTPQEISAMILQKLKAD
                     AEAYLGEQVTQAVITVPAYFSDSQRQATKDAGRIAGLEVLRIINEPTAAALAYGLDKE
                     HDQKILVFDLGGGTFDVSILEIGDGVFEVLATSGNNRLGGDDFDQRIIDYLAESFRKE
                     NGIDLRTDKMALQRLKEAAEKAKIELSGVTTTNINLPFITADASGPKHLDMTLTRAKF
                     DELTADLVEMTMGPTRQAIQDAGLTPEKIDKVLLVGGSTRIPAFQDAVKKYLGKEPFK
                     GINPDECVAIGAAIQAGVLAGEVKDLLLLDVTPLSLGIETLGGVFTKLIERNTTIPTK
                     KSQIFSTAADGQTSVEIHVLQGEREMAAYNKTLGRFHLTGIPPAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGNEQKITITASTNLSEDEIQRAVKEAEKYAAEDKKRKEEVDIRN
                     NADSMVYQSEKTLKELGDKLSPEDRSKIETAINNVKEALKGTDTARIKSATEALTQAF
                     YEISAKIYQQNPGAQGGPNPGAGFDAGQQAGPGMGQDNVYDADYKVVDEDDKK"
     gene            complement(3157..3846)
                     /gene="grpE"
                     /locus_tag="PLG72_09240"
     CDS             complement(3157..3846)
                     /gene="grpE"
                     /locus_tag="PLG72_09240"
                     /inference="COORDINATES: protein motif:HMM:NF010757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ81017.1"
                     /translation="MSDKRQKNQAEASSAENLNSTDSDVITGNMSGSAEESEAGDTAG
                     TDGKAETPDTEDGHGNTGGENETAETKALKEELEAVKSELEEQKKKCGEYLDRLQRTA
                     AEFDNFKKRTVKEKEALYDDAVCDVIREFLPVIDNLERAQNAVSADSSAQSLKDGVEM
                     VFKQFRDILKKIGVEEIKALNETFDPLLHNAVMHVEDESLGTSVIVEEFQKGYRYKDK
                     VVRHSMVKVAN"
     gene            complement(3961..>4961)
                     /gene="hrcA"
                     /locus_tag="PLG72_09245"
     CDS             complement(3961..>4961)
                     /gene="hrcA"
                     /locus_tag="PLG72_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOJ81018.1"
                     /translation="IDKAEPIGSRTIARKYELGLSSATIRNEMADLEEMGYLAQPHTS
                     AGRVPSDKGYRLYVDKLMPTRSLNNAEIRELRSAVNTRVYELSQLLKQATAVLSNFTK
                     YTSMAVTPKMRSSTIKTVQVVPIEAGKALVIVVNNAGIVRNALIKISEQATSEMLGRI
                     SNLLNDKLAGLTFEQADTEGIKTLGREIGVSGDIMQPILDGTADCIYQMGDHEMYVEG
                     STNILNHPEFKDMDKARQFLSMLDEKTSICRAVYDSTRPNGITVRIGSENDLSSIRDC
                     SLVTVSYNVSDTMVGTIGIIGPTRMAYNKVISSISYMKKLVSEEISKLLGHEPEDE"
BASE COUNT          934 a         1447 c         1121 g         1459 t
ORIGIN      
        1 ttttcctgaa tgtcttcctg accttgccca tacctttgca ttcagggcac ggatccgtta
       61 ttattttgcc ctcgccgcgg cacatgtcac acgtcctgac gtcgataaac tggccaaaag
      121 cagtggcatg cttgtgctgt atctgccccg tcccgccgca cttcttgcag gtggccggac
      181 ttgtgccggg cttcgatccg gaacctccgc atttcggaca gttctcatgc ctgctcatgg
      241 ttatttcctt ttcaacacca aaagcagcct cttcgaacgt gatctcgacg gaatacttca
      301 gatccgcgcc tttctgcggg cctgtgcggc tcctcgatga acgtccgaac ccggcgccgc
      361 cgaaaaacga ctcgaatgtt tcgaatatat cgctgaatcc accgaagtca aaacctccga
      421 agccggcacc ggcgctgccg aatccgttgg gatccacacc tgcatgtccg aactggtcat
      481 atcttgccct cttctgaggg tcgctcagca cttcgtacgc ttcatttatc tccttgaatt
      541 tcgcttccgc atccttgtcc ccggggttca tatcgggatg gtactgtttt gccagtttcc
      601 tgtaagcctt ttttatctcg tcctcatccg cgttccggtt taccccgagc acttcgtagt
      661 aatccctctt tgactgcggc aattctccac atctcctatc acaaaactat cttttcaccg
      721 aatactgttg cccatataag tataatagct catatgagta caatacaata tctcatgcaa
      781 attctcatgt aaactcatca aaaggggaca attgacactg tcccctttat cgcctgcttg
      841 ctcctggaag ccatgactcg taaagcttcc gctctgatga aatccatcgg ctttacgata
      901 aattccattt tcatgcctcg atattataac atacttttca tataaaattt tcgtttgcag
      961 aaaaaattta cttatccagc tgtattccaa cgaggcgacg gcaggacgtc catcgcctcg
     1021 ctgcatcatt acagcgtatt ttttacagga gttttctttt acaggaattt acccgcttca
     1081 gatcagccct tattttttat cgtcctcgtc aacgaccttg taatcggcgt catacacatt
     1141 atcctgcccc atcccgggac ctgcctgctg ccctgcatcg aagccggcac cggggttcgg
     1201 ccctccctgg gcgcccggat tctgctggta gatctttgcg gagatctcat agaacgcctg
     1261 cgtaagggct tcggtagccg atttgatcct tgccgtgtca gtgcctttca gcgcttcctt
     1321 tacattgttg atcgcagtct cgatcttcga cctgtcttca ggcgacagtt tgtccccgag
     1381 ctctttcagc gtcttctcgg actggtagac catcgagtcg gcattgttcc tgatatccac
     1441 ctcttccttg cgcttcttgt cctcggccgc atatttctcg gcttccttga ccgcccgctg
     1501 gatctcgtct tccgaaaggt tcgtcgatgc cgtgatcgtt atcttctgct catttcctgt
     1561 gccaagatcc tttgccgaaa catgcacgat accgttggcg tcgatatcga aggtaacctc
     1621 gatctgcgga actcccctcg gggcaggcgg tataccggta aggtggaatc ttcctagcgt
     1681 cttgttgtat gcagccatct ccctttcgcc ctgcagcaca tggatttcga cgcttgtctg
     1741 gccgtcggcc gcagtggaga agatctggct cttcttcgta ggtatggtgg tattcctttc
     1801 gataagcttg gtaaatactc cgccaagagt ctcgatacca agtgaaagcg gagtgacgtc
     1861 cagcagcagc aggtctttga cttcgcctgc cagaacaccg gcctggatgg cagcgcctat
     1921 ggcaacgcat tcatcagggt tgatgccctt gaacggttcc ttgccgaggt actttttaac
     1981 tgcgtcctgg aaagccggta tcctcgtgga tccgcctacc agcaatacct tgtcgatctt
     2041 ttccggcgtc agcccggcat cctgtattgc ctgccttgtc ggccccatcg tcatttccac
     2101 caggtcggcc gtcagttcat cgaacttcgc gcgggtcagc gtcatgtcca ggtgtttcgg
     2161 gcccgacgca tcggccgtga tgaacggaag gttgatgttt gtcgtggtta cgccggaaag
     2221 ctctatcttg gccttctcag cagcttcctt cagcctctgc agtgccattt tgtcggtcct
     2281 gagatctatc ccgttttctt tcctgaacga ttctgccagg tagtctatga tcctctggtc
     2341 gaaatcatcg ccgccgagcc tgttgtttcc gctggttgca agcacttcga aaacgccgtc
     2401 gccaatctcg agtatggaca cgtcgaatgt gccgcctcca aggtcaaaca ccaggatctt
     2461 ctggtcatgt tccttgtcaa gcccgtatgc aagggctgca gccgtaggct cgttgatgat
     2521 cctgagtact tcgaggcctg cgatccttcc ggcgtccttc gtagcctgcc tctgcgaatc
     2581 gctgaaatat gccggcacgg tgataacagc ctgcgtcacc tgttctccca ggtaagcttc
     2641 ggcatccgcc ttcagtttct gcaggatcat ggccgaaatt tcctgcggag tatagctctt
     2701 atcatctatc tttaccctcc tgtcggtgcc catgtccctc tttatcgata tgaccgtacg
     2761 ttcagggttg gttattgcct gtctttttgc aacctgtccg acaagtcttt caccggtctt
     2821 ggaaaaggcc acaacagacg gtgttgtcct gctgccttca ggattcggga taacgacagg
     2881 ttcgcctccc tccataacag caacacatga gtttgttgtg ccgagatcta ttcctattac
     2941 ttttgccata atgtacctcc ctgctaagtt atattagtat tggattgaaa ataatgatcg
     3001 ttcttaaata tgctatttat ctgccttgcg gtaatatgcc cggcagcagg catatggccg
     3061 gtacgcctga gcttgtgacc atccggctcc cacagcttgc ggtcatttcc tgcatcagcc
     3121 tgcggccgtc cgctgcagaa gcttcggtac cgccggtcag ttcgccacct tgaccatgct
     3181 gtggcgtacg accttgtcct tgtacctgta gcctttctgg aactcctcaa caatgacgct
     3241 ggtgcccagg gattcgtcct ccacatgcat caccgcattg tggagcaacg gatcaaaagt
     3301 ctcattgaga gcctttatct cctctactcc gatttttttc aatatgtccc tgaactgttt
     3361 aaagaccatt tcaacgccat ctttcagaga ctgcgccgag ctgtcggctg atacggcatt
     3421 ctgcgctctt tcaagattat cgataacagg caggaactcc ctgatcacgt cgcagacagc
     3481 atcgtcatac agggcttcct tctctttaac cgttcttttc ttgaaattat cgaattctgc
     3541 ggctgtcctc tggagtctgt ccaggtattc gccgcatttt ttcttctgct cttcaagctc
     3601 tgactttaca gcttccagtt cttctttaag ggctttggtt tccgctgtct cgttttcgcc
     3661 gccggtattg ccgtgcccgt cttcggtatc gggcgtttca gccttaccgt ctgttccggc
     3721 tgtatcgccg gcttcgcttt cttccgccga tcctgacatg ttgccggtga tcacgtcact
     3781 gtccgtactg ttcaggtttt cggcgctgct tgcttcagct tggttcttct gtcttttatc
     3841 gctcatttcg ctaaatcatc ctttgacaag tatttccctt ccagggatat taaaaatcgt
     3901 ccggacagac ctgccgcagg catctgccgg accggcggcg ccatgccgct gtccgatact
     3961 ttactcgtct tccggctcat gccccaggag tttgctgatc tcttcactca ccagcttttt
     4021 catatagctg atggacgaaa tgaccttgtt gtacgccatc cttgtagggc caatgatgcc
     4081 gatagttccc accatcgtgt cagatacatt ataactgacc gtaaccaaac tgcaatccct
     4141 gatgctggaa aggtcgtttt ccgatccgat cctgacagtg atgccgtttg gccgggttga
     4201 atcgtatact gccctgcata tggaggtctt ttcgtcaagc atgctcagga actgccttgc
     4261 tttgtccata tccttgaatt caggatgatt gagtatattt gtggaaccct ccacatacat
     4321 ctcatgatcc cccatctgat atatgcagtc ggcagttccg tccagtatcg gctgcatgat
     4381 atccccggac acgccgatct ctctgcccag ggtctttata ccttcagtat cggcctgttc
     4441 gaacgtgaga cctgccagtt tgtcattcaa caggttcgat atccggccaa gcatctccga
     4501 ggttgcctgc tctgatatct tgatcagggc gttccttacg atgcccgcgt tgtttacgac
     4561 tattaccagc gcttttcccg cttctatcgg aacgacctgt accgttttta tcgtactgct
     4621 cctcatcttc ggtgtcactg ccatggatgt atacttggtg aagttcgata ggacagcggt
     4681 agcctgcttc agcaactggc tcagttcata tacccgtgta ttcactgctg acctcagttc
     4741 ccttatctct gcattgttca ggctgcgcgt cggcatgagc ttgtcgacat acagcctgta
     4801 ccccttgtcc gatggcacgc ggcctgcgga agtatgcggc tgcgcaagat agcccatctc
     4861 ttcgaggtcc gccatctcgt tcctgatcgt agccgaactc agcccaagct cgtatttcct
     4921 ggcgattgtc cttgagccga taggttcggc cttatcgatg t
//
LOCUS       DAOSCC010000186         4932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10792_length_4932_cov_4.555259, whole genome shotgun sequence.
ACCESSION   DAOSCC010000186 DAOSCC010000000
VERSION     DAOSCC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10792_length_4932_cov_4.555259"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLG72_09250"
     CDS             <1..529
                     /locus_tag="PLG72_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81019.1"
                     /translation="EKLREHGSFPDKVILAGHSAGAHLVTFLAYSDFLDKTGFDKNMI
                     KGVISISGPINFAECKNSFVSKVISDFAATDENKAKADPYPYLKDDADIPILCIHGDR
                     DPVVEPANSISFVNRINEIRDGLGELIMIRGGLHTNIAKLFWNGLDEHARMDEWILKV
                     ERPAPYDGTMPASNT"
     gene            complement(694..1473)
                     /locus_tag="PLG72_09255"
     CDS             complement(694..1473)
                     /locus_tag="PLG72_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ81020.1"
                     /translation="MGYFEQLIHEIDGFRFSGWDFSYITGTGRMQEAPVKWNYYSRIR
                     PYLYNAERLLDLGTGGGEKLKAFVPLPGETYATEGYKPNVEIAKRNLEPLGVTVVEVD
                     GDEGPPYNSNLPFDDDFFDLVTDRHEAYCPQEIYRILKDDAHFITQQVGSLSIANLIQ
                     LFDNKNIQISDWNLNYAEKELMQNGFEIAGSDEEISFIRFYDVGALGIYMKAFPWVFP
                     QFSAKKYAEELLYIHKRILNDGYIDIVYHLFFIDAVKRKKI"
     gene            complement(1673..2668)
                     /locus_tag="PLG72_09260"
     CDS             complement(1673..2668)
                     /locus_tag="PLG72_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HOJ81021.1"
                     /translation="MNFDAVYYEPDSLRYELGKMLKQKYQSLPWIEIESHNRIPEFTS
                     AENREFPKLKRHLVIGIRKTHKYVENHKVSDWLVPYTSSGCRAMCLYCYLVCNYNKCS
                     YLRLFVNREQMMLKLLKTAREASVPQTFEIGSNSDLVLENTITGNLNWTIDYFARNGR
                     GFLTFPTKFDMVEPLLDLDHRGKTIFRMSVNPTEIIRKVEIGTSPLETRVRALNNMAE
                     AGYPVGMLIAPVILVQDWKKIYGELIEYLADELSQHVKKTGFIEIILMTYSYVHNAIN
                     SEAFPGAMKIYDHCLMTGRGRGKYSYRQEVREDAGDFLRNELGHKLGSMKILYIS"
     gene            complement(2905..3411)
                     /locus_tag="PLG72_09265"
     CDS             complement(2905..3411)
                     /locus_tag="PLG72_09265"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOJ81022.1"
                     /translation="MKAILSADLNWGIGRGGKLLVRVPDDMKFFWKMTTGKVVVMGRD
                     TFESLPGREPLRDRVNIVLSRSADYDDERLIICRSLEELFDELEKYDTDDVFVIGGES
                     VYTQLLPYCSDVYITRFEKVFEADRHFPNLDAMENWVLVEESERQFYNDMGFRFLKYI
                     NKSVREYR"
     gene            complement(3494..4324)
                     /gene="thyA"
                     /locus_tag="PLG72_09270"
     CDS             complement(3494..4324)
                     /gene="thyA"
                     /locus_tag="PLG72_09270"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOJ81023.1"
                     /translation="MSKADIIFKEMCKDILENGFSSEGQTVRARWEDGTPAHTIKKFG
                     VVNRYDLSEEFPIMTLRYTNLKAAIDELLWIWQKKSNNMRDLNSHIWDSWADENGSIG
                     KAYGYQMRIKHKYPEGEFDQVDRVLYDLKHNPYSRRIITNMYVHSDLHEMNLYPCAYS
                     VTFNVTGKKLNAILHQRSNDLLVANNWNVCQYAILVHMFAQVSGLEAGELVHVIADAH
                     IYDRHIPMVKELLDREPYPAPKLIINPDVKDFYNFTVDDFVLENYKHWPQIKNIPVAV
                     "
     gene            complement(4551..>4932)
                     /locus_tag="PLG72_09275"
     CDS             complement(4551..>4932)
                     /locus_tag="PLG72_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ81024.1"
                     /translation="RTSIGCYERTLALLIEKYAGALPTWIAPVQVKVLPIVDKHHDYA
                     RQVAGKLQDAGIRAEVDYRNEKIGYKIREAQMEKVPYMLVLGDKEAENGQVAVRSRKD
                     GDLGPMLLDDFIARITEEVRSKAK"
BASE COUNT         1151 a         1313 c         1068 g         1400 t
ORIGIN      
        1 ggagaaactc agggaacatg gatcgtttcc cgataaagtc atccttgccg gacactcggc
       61 tggcgcccac ctggtaacat tcctggcata cagcgatttt ctggataaga ccggattcga
      121 taaaaatatg atcaaaggcg tgataagcat aagcgggccg ataaatttcg ccgaatgcaa
      181 aaacagcttc gtcagcaaag tgatcagcga cttcgcggca actgatgaaa acaaggcaaa
      241 ggctgatcca tatccatatc tgaaagacga cgccgacata cccatactgt gcatacacgg
      301 agatcgcgat ccggtagtcg aaccggccaa ctctatttca tttgtgaaca ggatcaacga
      361 aatcagggat ggacttggcg aacttatcat gatcaggggc ggcttgcata cgaacattgc
      421 caagcttttc tggaacggcc tggatgaaca tgccaggatg gacgagtgga tcctgaaggt
      481 tgaaagaccg gctccatacg acggcacgat gcccgcttca aatacttgat ccatcagtcc
      541 cgcataggca tggcatatcc tggaacatcc tgatcttacc gcagcttccg gtgccagttt
      601 ggcctgacaa tgccgtcctt ttgccatggc caggtataaa gggagccgca gatccacacg
      661 cctcccacgc cgggaaccct aactgcagcg gtgttatatc ttctttcttt ttactgcatc
      721 aatgaaaaac aaatgataaa cgatatcgat atatccatca ttcaaaatcc gcttgtgtat
      781 atacaggagc tcttctgcat attttttcgc agaaaactgc ggaaacaccc acgggaacgc
      841 tttcatgtaa atgcccagtg ccccgacatc atagaacctg atgaaactta tttcctcatc
      901 tgaaccagcg atttcaaatc cgttctgcat cagttccttt tccgcatagt tcaggttcca
      961 gtctgaaatt tgaatgtttt tgttgtcgaa caactggatc aggttcgcga tggaaaggct
     1021 tccaacctgc tgggtgatga aatgtgcatc atctttcaaa atccgatata tttcctgagg
     1081 gcaatatgct tcatgtctgt ccgtgaccag gtcgaaaaag tcatcgtcaa aaggcaggtt
     1141 actgttatat ggcggccctt catcaccatc gacttctact accgttacac caagaggttc
     1201 gagatttctc ttcgctattt caacattggg cttgtatcct tccgttgcat acgtttctcc
     1261 cggcaaaggt acaaacgctt tcaacttttc acccccgccc gtgcccaggt ccaatagtct
     1321 ttcagcattg tacaggtatg gccttatcct gctgtaatag ttccatttta caggggcttc
     1381 ctgcatcctg cctgttcctg ttatgtagga aaaatcccat cccgaaaacc tgaagccgtc
     1441 aatttcatgg atcaactgtt caaaatagcc cataatcatc ccgtcctttt gcggtatcat
     1501 ccttccggta tctgcggaaa tattccggcc acgtcatttt cagccgtttt ttaatattat
     1561 actttcatac gagaattttc caaatgactt ttcgaccctg tcagaacaat cagtgacttt
     1621 ttagcttcgg atctgatata tagccggtct ttgccgtttc ctgccgacgt gctcatgaaa
     1681 tataaaggat tttcatgctg ccgagtttat gcccgagttc attgcgaaga aagtccccgg
     1741 catcttccct gacctcctga cgatacgagt attttcctct tccccgccct gtcatcaggc
     1801 aatggtcgta tattttcatt gcccccggga atgcctcgga atttattgca ttatggacat
     1861 aactgtacgt catgaggatt atctctataa atccggtttt tttaacatgt tgagacaact
     1921 catctgcaag gtactcaatg agttcgccgt atatcttctt ccagtcctgt accaggataa
     1981 ccggagctat cagcatccct acgggatacc cggcttccgc catattattg agagcccgta
     2041 cgcgtgtttc gagcggtgaa gtcccgatct ccacttttct gatgatctcc gttggattca
     2101 cgctcatacg gaaaatggtt tttccccggt ggtccaggtc cagcagcggt tcaaccatgt
     2161 cgaacttcgt ggggaacgtg aggaagcccc gcccgttcct ggcgaaataa tcgatcgtcc
     2221 agttgagatt ccccgttatg gtattctcca gcaccaggtc gctgttgctc ccgatttcaa
     2281 atgtctgggg cacagatgcc tccctggccg ttttcagcag tttcagcatc atttgctcgc
     2341 gattgacgaa caaccgcaaa taggagcatt tgttgtagtt gcacacaaga tagcaataca
     2401 ggcacatggc ccggcagccg gaggatgtat atggcaccag ccagtcggat actttatggt
     2461 tctcaacata cttatgggtc tttcggatcc caattacgag gtgtcttttg agcttcggga
     2521 attcccggtt ctctgccgaa gtgaactcag ggatgcgatt atgcgactcg atctcgatcc
     2581 acggcagcga ctggtacttt tgcttcagca ttttcccaag ctcatacctc aatgaatccg
     2641 gctcataata aaccgcgtca aagttcatat ccatatcgat ttccaccatg ctgtgatata
     2701 tccggcttta gcttattatc agcgatgctt tccgataata tacacctgca ttggtggcat
     2761 aacatccgtg tcgaaaaggc attggtggta taacgtttgt gtctggttga agtgctgaga
     2821 tggctgggca gcccggacca cgtttataat gtcaaccgct ggcattttat catcaaaacg
     2881 ctcatcaata tttccagacc gttttcatcg atattcccgg actgacttat tgatatattt
     2941 caaaaaacgg aatcccatat cgttgtaaaa ttgccgttcg ctttcttcaa ccagcaccca
     3001 gttctccatg gcgtcgaggt tcgggaaatg ccggtcggct tcaaaaactt tttcgaaacg
     3061 cgtgatatat acatcggagc aatatggcag aagctgtgta tatacagatt cgccgccgat
     3121 cacaaaaaca tcgtcggtgt cgtatttctc aagttcatcg aaaagctctt ccagcgagcg
     3181 gcagataatg agcctctcat catcgtagtc cgcgcttctg ctcagcacta tgttgaccct
     3241 gtccctgagc ggttcccttc cgggcaatga ctcgaatgtg tccctgccca tcacgacgac
     3301 ctttccggtc gtcatcttcc agaaaaactt catatcatcg ggaacccgca ccagcagttt
     3361 cccgccgcgg cctatgcccc agttgagatc tgccgatagt atagccttca tggtccgctc
     3421 ctttccattc ttccagggac attcctcagc tgcgaagcat tgctccatgc agaggagtgt
     3481 ccctgcacct ttttcacacc gccacgggga tattctttat ctgcggccaa tgcttataat
     3541 tctccagtac aaagtcatcc acggtgaagt tgtagaaatc cttaacatcc ggatttataa
     3601 tcagtttcgg cgctggataa ggctcacggt caagcaattc ctttaccatt gggatatgcc
     3661 tgtcgtaaat atgggcatcg gcgatcacgt gcacaagttc gcctgcttcc agcccgctga
     3721 cctgtgcgaa catgtggacc aatatggcgt actggcagac attccagttg ttagccacaa
     3781 gcaggtcatt ggaacgctgg tgcaatattg cgttgagctt tttacccgtg acgttgaaag
     3841 tcacgctgta tgcacacggg taaaggttca tttcatgcag gtcgctgtgg acatacatat
     3901 tggtgatgat ccgccggctg tacgggttgt gtttcaggtc gtacaatacc ctgtccacct
     3961 ggtcgaactc gccttccgga tacttgtgtt ttatccgcat ctggtaaccg tacgctttgc
     4021 cgatcgatcc gttttcgtcg gcccaactgt cccagatatg gctgttaagg tccctcatat
     4081 tgttggattt cttctgccat atccaaagca gttcatctat ggccgctttg agattcgtat
     4141 atcgtaacgt cattatcggg aattcttcag acagatcata cctgttgacg acaccgaact
     4201 tctttatcgt gtgagcaggc gtcccgtcct cccatctggc acgcactgtc tggccctctg
     4261 acgaaaatcc gttttccagg atgtctttgc acatttcctt gaagataatg tccgctttgc
     4321 tcatatctct gcccaccgcc atttccttcg tatttaaaaa tattatacct ttcgggcgta
     4381 atttttccaa ctgtttatta ccgacgaagc taagccatca atgacatctc ctgacagtct
     4441 ccctggcaag gctatgcaca gtgatccctg taaaatgaaa aggcatggct ccctgtccat
     4501 gcctttccgt ttttatatct tctgatgttt ttcatttcat acatactttc ctattttgcc
     4561 tttgacctga cttcctcggt tatcctggct atgaagtcat cgagaagcat agggccaagg
     4621 tctccgtcct tgcgggacct tacggccacc tggccgttct ccgcttcctt gtctcccagg
     4681 acgagcatat acggaacttt ttccatctgt gcttccctga tcttgtatcc gatcttctca
     4741 ttcctgtagt cgacttccgc cctgataccg gcatcctgga gcttccctgc cacctgccgt
     4801 gcataatcgt ggtgcttgtc gactatgggc agcaccttca cctgtaccgg agcgatccag
     4861 gtcggcagtg cgccggcgta tttttcgatc agcagcgcaa gcgtcctttc atagcatccg
     4921 atagacgtcc tg
//
LOCUS       DAOSCC010000187         4885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_10894_length_4885_cov_3.640373, whole genome shotgun sequence.
ACCESSION   DAOSCC010000187 DAOSCC010000000
VERSION     DAOSCC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_4885_cov_3.640373"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PLG72_09280"
     CDS             <1..1193
                     /locus_tag="PLG72_09280"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359673.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOJ81025.1"
                     /translation="YFVDKNGKKYVEGDWISLDGSTGNVYGEQLPTVDPEMAGDFATL
                     MSWADEIRALKVRTNADTPKDAAVAVRFGAEGIGLCRTEHMFFESDRIFAFRQMIVSS
                     NAEQRKKALEKILPMQRGDFEALFTAMKGHPVTIRLLDPPLHEFLPQDEGEIKELADE
                     LGISADELKNVIKNLHELNPMMGHRGCRLAVSYPEIAEMQTRAIIEAAINVNKKGMNV
                     TPEIMIPLVCELKEMKFVKDIIVKTADAVIKEAGVSLEYKVGTMIEIPRAALLADEIA
                     KEAEFFSFGTNDLTQMTYGLSRDDAGRILEDYYQAKIFEFDPTARIDQSGVGKLMKIA
                     VEGGKATRPDIKLGICGEHGGDPSSIEFCHNIGLNYVSCSPFRVPIARLAAAQAQVKN
                     PRSK"
     gene            complement(1500..2321)
                     /locus_tag="PLG72_09285"
     CDS             complement(1500..2321)
                     /locus_tag="PLG72_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81026.1"
                     /translation="MSKTLKNIIATIVICAVVVTAALMIYDRYVRGRDGETNHEPAPQ
                     EQTDNGQAKDGEDQKDGNTAGNGTDETGDATDGTNDSKDGSPADEIDKELLYDENGII
                     KPEYAEKIIKATAAKVIQALKAKDAQAISDFVHPVNGLRFTPYTSVDLKQDLVFTAEE
                     VKNFFNDKKKYMWGYYDGTGDEIILTPAEYYDVFVYSNDFANAEKIGYNEVLSYGNAV
                     ENQFEAYPGSIIVEYYFSGFDPKYEGMDWQSLRLVFEEYKGGWKLSGIIHNQWTI"
     gene            2876..>4885
                     /locus_tag="PLG72_09290"
     CDS             2876..>4885
                     /locus_tag="PLG72_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOJ81027.1"
                     /translation="MPCRRGQVSRYNGSLRSSGPFMGGAGSHPALRTAVRVIAVSLLL
                     CAAMMIPFASAAPEDEPDSVLVIHSYDQGLAWTYRQNEGILNTLAGAEESLQVYVEYM
                     DSRNYPDSGNLERFREYCRYKYSDREIDIVITTDEAALRFALDNRAELFSDAPVVFSG
                     VSESAAKEIIGDRTGVTGIVEEIDAVGTVKAAFGINPGIKKIYVIFDDSANSVIQGQS
                     ALEAIKEYAAKAEVVALSSSDTAKVLDSVSNAEDNSIVVFSALTAGHAADMSGFNHFC
                     AALGRESRAPVYHLYDTGFGNGAVGGSMVSGAVQGEEAAKLALRILEGEDISGMPFEK
                     KNTTRYVFDYKALQRFNIDTALLPPGSEIINKPGSYLEDHRHTVLVAVVVIVMLLIFI
                     TVLLFYLKRLQTMKKELSQSNENLTGLYEDLSLASRKLKKQYDELIAVQKDLSISEYK
                     FELLFEKMMDGFFIFEPVYNSVGKIVDIRFLMVNPGFFRNFGMEKTMVVGKTWLEVFG
                     SPNRNLARYQNLLETGRSERFESYDPQSRKYYLVEMFLIEKNQAGVIFENITDYKKAL
                     KEVRQLNAELEKRVADRTAKLREAVAELESFAYTVSHDMKSPMRAVDGYVQILLEDFG
                     DKLDSDAVQMLNNIRTISRESIDMINMILQYSKTSAAVLNKEPVDM"
BASE COUNT         1294 a         1110 c         1287 g         1194 t
ORIGIN      
        1 agtatttcgt tgacaagaac ggaaagaagt acgtagaagg cgactggatc tcactggacg
       61 gctccaccgg aaacgtatac ggcgaacagc ttccgacagt cgatcctgaa atggcgggcg
      121 actttgcaac acttatgtca tgggcagacg agatcagggc cctgaaggta aggaccaatg
      181 cggatacacc caaggatgcc gcagttgcgg tcaggttcgg tgcagaagga ataggcctgt
      241 gccgtaccga gcatatgttc tttgaatccg acaggatatt cgcattcagg caaatgatcg
      301 tctcaagcaa tgccgaacag aggaagaagg cccttgaaaa gatcctgccg atgcagagag
      361 gcgatttcga agcactcttt actgcgatga agggtcatcc tgtgaccata aggctgctgg
      421 atcctccgct gcatgaattc ctgccccagg acgagggaga gatcaaagaa ctggccgatg
      481 aactcggcat ctctgctgat gagctcaaga acgtcataaa gaacctgcac gaactcaacc
      541 cgatgatggg ccacaggggc tgccgtctgg ccgtctcata tcctgagatc gccgagatgc
      601 agacaagggc tatcatcgaa gctgcgataa acgtcaataa gaagggcatg aatgtgactc
      661 ctgagatcat gatcccgctg gtttgcgaac tcaaggagat gaagttcgtc aaggatatca
      721 ttgtaaagac tgctgacgcc gtcatcaagg aagccggagt cagcctggaa tacaaggtcg
      781 gaacaatgat cgagatcccg agggcagctt tgctggctga tgagatcgca aaagaagcag
      841 agttcttctc cttcggtaca aacgacctga cccagatgac ttacggcctg agccgtgacg
      901 acgccggaag gatccttgaa gattactacc aggcaaagat attcgaattc gacccgactg
      961 caaggataga ccagtcaggc gtcggcaagc tgatgaagat agctgtcgag ggcggaaagg
     1021 caacaaggcc cgacatcaag ctcggcatct gcggagagca cggcggagat ccgtcatcaa
     1081 tagagttctg ccataacatc ggcctgaact atgtttcatg ctcgccgttc cgtgtgccga
     1141 tagcaaggct tgcagccgca caggcgcagg tcaagaatcc gaggagcaaa taaatgaaac
     1201 ggtgaacctg gtgtatcagg caatcaaaaa ccggtatggc atataatgct gtaccggttt
     1261 ttcatatact ttagcggtcg gacttatggc gaaggtctcg tctatctgtg gaatgctttc
     1321 acacactcgt atacctgatc tttcctgcgt aaaatttttc ttatacctgt ttaaagaaaa
     1381 tcccgctttg aacaggtatg ttcatgaaag gttctgtcat aaattcacat tcgcccggga
     1441 atggttctgc tctgaggcgc tgatttcatc caaagtatgt ctgcatttgc aataattcct
     1501 cagatagtcc actggttatg gatgatgcct gacagtttcc agccgccttt atattcttcg
     1561 aacaccagcc tgaggctttg ccagtccatg ccctcatact tcgggtcaaa tcctgagaag
     1621 tagtattcca cgatgatcga accggggtag gcttcaaact ggttttcaac agcgtttcca
     1681 tagctgagca cttcattgta tccgatcttt tcagcattgg cgaagtcatt tgaataaacg
     1741 aaaacatcgt aatattcggc gggcgtcagg atgatctcat cgcctgtgcc gtcgtaatag
     1801 ccccacatat atttcttttt gtcattgaag aaatttttga cttcttctgc agtgaatacc
     1861 aggtcctgct tcagatcaac agaagtatag ggggtgaacc tcagaccgtt cacgggatgt
     1921 acaaagtccg agatggcttg cgcgtctttg gctttaaggg cctggatgac cttagctgca
     1981 gttgctttta tgatcttttc ggcatattca ggtttaatga tcccgttttc atcataaagc
     2041 agttccttgt cgatctcgtc ggccggagaa ccatccttgc tgtcattggt cccatcagtg
     2101 gcatcgccgg tctcatcggt accgtttccg gctgtgtttc cgtccttctg atcttcgccg
     2161 tcttttgcct ggccattgtc agtctgctcc tgcggggcag gttcatgatt tgtttctcca
     2221 tccctgccgc gtacgtagcg gtcatatatc atgagcgctg cagtaacgac gacagcacaa
     2281 atgactattg ttgcgatgat gttcttcaat gttttgctca ttatagagta cctccgtatg
     2341 ttcatataat acaatgacga aaaccacagg atattgttcc gtgcatgcaa atccactatg
     2401 ctgccttgaa tgcaatgtat aatcctggtc atgaacttcg atttttccca tgtcgtctat
     2461 tatatcagtt attatttctg gaaatggagt aaaatattat aaaaaagtgt ttcattgggt
     2521 atttcctgat catggtattg ctgctcatac cagggtgttc cgtaaataag gacttagaga
     2581 aggacagccg gcagcaaact gaccgtcttg ttcaggaaaa tcgccggcag atcgcggatc
     2641 aggatggatg caaaaacacg agacagacgg atcatgaccg attcccgacg acctttggtt
     2701 tgtcagtaca gggtggtgca gttattcgat gctccaccct gttttatgcc tgaatatgct
     2761 cagatttgca aattctatga aggatattga tataatttgt tgaataaata aatatgagtt
     2821 tttgctgtaa atggagaatc attgcaaaat tttaacgaaa gcagagagaa acgtaatgcc
     2881 atgcagaaga ggccaggtca gcagatataa tggatcgctc aggtcttccg gcccgtttat
     2941 gggaggcgcg ggcagtcatc cagccctcag gacggcggta cgtgtcattg ccgtttccct
     3001 gctgctgtgt gctgcaatga tgataccctt tgcttcagca gcgccggaag atgagccgga
     3061 cagcgtgctg gtcatccatt catacgacca ggggcttgcc tggacgtaca ggcagaacga
     3121 agggatactc aatactcttg ccggcgccga agaaagcctg caggtatatg tggaatacat
     3181 ggatagcaga aactacccgg acagcgggaa tctcgagcgg ttcagggaat actgccgcta
     3241 caagtattcg gacagggaga tcgatatcgt tataaccacg gacgaggccg cgctccggtt
     3301 tgccctggac aaccgcgccg aactgttttc ggatgcgcct gtcgttttct cgggtgtaag
     3361 cgagtcagcg gcaaaagaga tcatcggcga caggacgggc gtaacgggca ttgttgagga
     3421 gatcgacgcg gtcggcactg tgaaggcggc ttttgggata aatcccggca taaagaaaat
     3481 atacgtcatt tttgacgaca gcgccaacag cgtcatacag ggccaatcag ccctggaagc
     3541 aataaaggaa tatgcggcga aggcggaagt ggtcgcgctg agcagcagtg acactgcgaa
     3601 agtactggat tcggtcagca atgcggaaga taacagcata gtagtattct cagcgctgac
     3661 agccggacat gctgcagata tgtccgggtt caaccatttt tgcgccgctt tgggcaggga
     3721 aagcagggcg cccgtctatc acctgtatga tacggggttt ggaaacggcg cggttggcgg
     3781 gagcatggtt tcaggcgcag tgcagggtga agaagctgca aaacttgcat tgaggatact
     3841 tgaaggcgaa gatatatcgg gaatgccctt cgaaaagaag aatacgacca ggtatgtgtt
     3901 cgattacaag gcgcttcagc gttttaacat cgatacggcc ctgctgcctc cgggcagtga
     3961 gatcataaac aagcccggtt cgtatcttga agatcacagg cacacggtcc ttgtcgccgt
     4021 agtcgtaata gtgatgctgc ttattttcat cactgtcctg ctgttttatc tgaaaaggct
     4081 gcagacgatg aaaaaagagc tttcgcaaag caatgaaaat ctgacgggac tgtatgagga
     4141 cctgtctctt gccagcagga aactcaaaaa gcagtatgat gaactgatag cagtccagaa
     4201 ggatctgagc ataagcgagt acaagtttga actgctcttt gaaaaaatga tggacggatt
     4261 cttcatattc gagcctgtgt acaattcagt tggaaagatc gtcgatatac gtttcctgat
     4321 ggtgaacccc ggattcttcc ggaattttgg catggaaaaa acaatggtcg tcggcaagac
     4381 ctggcttgag gttttcggaa gcccgaacag gaacctggca cgttaccaga atcttctcga
     4441 gacaggacgg tcggagaggt ttgagtctta tgatccccag agcaggaagt attaccttgt
     4501 tgaaatgttc ctgatagaaa agaaccaggc aggagtcata ttcgagaaca tcacggacta
     4561 caaaaaagcg ctgaaagagg tcaggcagct taacgccgaa cttgaaaaac gcgtggccga
     4621 caggactgca aagctgaggg aggcggtggc cgaactggag tcctttgctt atacggtatc
     4681 gcatgatatg aaatcgccga tgagggctgt cgacggttat gtccagatac ttctcgagga
     4741 cttcggggac aagcttgaca gtgacgctgt ccagatgctg aacaacattc gcaccataag
     4801 cagggaatcc atcgatatga tcaatatgat actgcagtat tcgaagacat ctgcggctgt
     4861 acttaacaag gagccggtgg atatg
//
LOCUS       DAOSCC010000188         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11119_length_4816_cov_3.616467, whole genome shotgun sequence.
ACCESSION   DAOSCC010000188 DAOSCC010000000
VERSION     DAOSCC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_4816_cov_3.616467"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..756
                     /locus_tag="PLG72_09295"
     CDS             88..756
                     /locus_tag="PLG72_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ81028.1"
                     /translation="MNALEIYLRQDGYNVIKASNGVQALEILKTSVVHLILLDVMMPQ
                     MDGIMTAVKIRENNINIPIIFLSAKSEDTDRVLGLNMGGDDYVTKPFNIVELLARVRA
                     HLRRYARLGGLAVQTAECLNVYRTGGLVVDDNKKKVTVDGDEVQLTATEYNILKLLIS
                     NPERVFSASQIYEAVWNEPAFNVGKIVSVHIRHIREKIEIDPKEPRYLKIVYGLGYKV
                     VQID"
     gene            879..2144
                     /locus_tag="PLG72_09300"
     CDS             879..2144
                     /locus_tag="PLG72_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ81029.1"
                     /translation="MSWDADSWATVAFVSGVVLLPIFAASIWIQVKNGRLVLWRSSKP
                     EFIIITVLVTLLIAIIYNLVRYDRFMNLILRILVLPFAAYILTVFWFVRLTAFLQTGG
                     RYKKSSWYKFYMRFPINQPISVFVTGLLLMSPVYLLLDLNTGTFILAISHFALVTALA
                     EYLSKFSETMAARFEKIAEDRLRAERMKTELIANVSHDIKTPITSIINYADLISSEST
                     VNERLKEYTSVIRRKSLRLKSLMEDLIEASKAGTGNIKMTLETIDFGELLGQIMGEYD
                     EQMEKNHLQLIAKIPETPVSIQADGRYLWRVLENIFSNAVKYSMSGTRVYADLTAAGT
                     AAGSTAGGDMAVGGMHVFTLKNISKDPLNISADELTEQFVRGDRARYTEGNGLGLYIA
                     KSLTEAMGGRFSININGDLFEVQIAFKSA"
     gene            complement(2193..2705)
                     /locus_tag="PLG72_09305"
     CDS             complement(2193..2705)
                     /locus_tag="PLG72_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81030.1"
                     /translation="MYKKILYKKVYRLLKDSTPLKFDCGLICSRKCCSGDNEAGMCLY
                     PGEESLLEGHDDFLNIQKTKMRDTDVLFAVCSGKCNRRFRPLACRIFPYAPYIDESGS
                     LTVIEDPRAKYLCPLLMESFGLKIDRRFKRNVTKVFRILIMDEEIKSFIGLLSAVLDD
                     YRKLTENPVH"
     gene            3154..>4816
                     /locus_tag="PLG72_09310"
     CDS             3154..>4816
                     /locus_tag="PLG72_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ81031.1"
                     /translation="MSAMKRMNIDRGWYLDTKQSGFNDFVNVRNMRQVDLPHDLMLES
                     EVSPDVEGGRASGYYKGGLAYYTKFITIPEELKDQRILVEIDGSYMNTEVYCDGNFVK
                     RHPYGYTPFHADITPYVKFGRETRLQVIVNNPFSFTSRWYTGTGIYRHVDILTAPYVH
                     LSPWPIFAHTDRVDGGTAYITVEVTAENHTAKPGIWHIDVELCTDDEGTVAATGRMAV
                     QIPAMGKRTGRVGIVVEDARLWDIDHPNLYKVKAIMREFSETRDDTQVIDAAETLFGI
                     RTISVDTKNGFMLNGRSLKLKGGCVHHTNGILGATSLYDCEYRRVKLHKDNGFNAIRC
                     AHNPPSRDFLEACDRLGIMVIDEAFDMFRMSGNPNDYHAYFETHWKYDLEQFITRDRN
                     HPCIIMWSALNEVQERFGLSDGYAVCADIADYIRKLDSTRPVTGAVCIPFNGLADDDA
                     AKLYKSWEEMDELKKQGNATLQNAYVPFGDKIWGSRTEPFVAPLDVVGYNYLDYRYKS
                     DHELYPNRIIVGTESFPKDIAATWKRVEECPHVIGDFTWTSMDYIG"
BASE COUNT         1317 a         1092 c         1182 g         1225 t
ORIGIN      
        1 aagaccgaaa aacccattga catgttcccg taaacgtaag gttcaaaaaa tcagcccaag
       61 acatcaatcc aagcggctcg ggccatattg aatgcactgg aaatatacct caggcaggac
      121 ggatataatg tcataaaggc atcaaacggt gtacaggctt tggaaatact gaaaaccagc
      181 gttgtacacc tgatactgct cgatgtcatg atgccgcaga tggacggcat catgactgcc
      241 gttaagatcc gcgagaacaa tatcaacata ccgatcattt ttttaagcgc aaaaagtgag
      301 gatacggaca gggttttggg ccttaatatg ggcggcgacg attatgttac gaagcccttt
      361 aatattgtcg aactgcttgc cagggtgaga gctcatctga gacgttatgc caggcttggc
      421 ggccttgcgg tgcagactgc ggagtgcctg aacgtataca gaacaggcgg gcttgtggtc
      481 gatgataaca aaaagaaggt tactgttgac ggtgacgaag tacagctgac cgcaacggaa
      541 tacaacattc taaagctgct tatcagcaac ccggagcgtg ttttttcagc cagccagata
      601 tacgaagcgg tatggaacga gcccgctttc aatgtcggca aaatcgtttc ggtccatata
      661 aggcacatca gggaaaagat cgaaatcgac ccgaaagagc caagatacct gaaaatcgtc
      721 tatggccttg gatataaggt ggtgcaaatt gattgaagaa gctacttgat tcatatcttt
      781 cctttatcct gctgctggct gtattattct gctgtgcctt tttagttgtg tgcagtatat
      841 tcagtcactt tagatattat gcaatagagt tgctccaggt gagctgggat gctgacagtt
      901 gggcgacagt cgcatttgta tctggcgttg tcctgctccc catctttgct gcatcgatat
      961 ggatccaggt caaaaacggc aggctggtcc tttggagatc atcaaaaccg gagttcatta
     1021 tcattactgt cctggtaact ttactcatcg caataatcta caaccttgta cgatatgaca
     1081 ggtttatgaa tctgatcctg aggatccttg tactgccttt tgcagcttac attcttaccg
     1141 tattctggtt tgtaaggctg accgcattcc tgcagacagg cggcagatac aagaaatcct
     1201 cctggtataa gttttatatg cggtttccga taaaccagcc gatttctgtt tttgttactg
     1261 gccttttgct gatgtcgccc gtttatttgc tgctggatct gaatactggt acgtttatcc
     1321 tggcaatatc ccattttgct ttagtaacgg cattggcaga atacctgtca aagttttccg
     1381 aaaccatggc cgcccggttt gagaaaatcg ccgaggacag gctgagagcc gagaggatga
     1441 aaacagagct tattgccaat gtatcacatg atataaaaac gcctatcact tccatcataa
     1501 actatgctga ccttatcagc agcgaaagta ctgtgaatga gcggctgaag gaatatacat
     1561 ctgttatccg caggaagtcg ctgaggctca agtccctgat ggaggacctg atcgaagcca
     1621 gcaaagcggg gacgggcaac attaaaatga cattggagac catcgatttt ggcgagcttt
     1681 tggggcagat catgggggag tatgacgagc agatggagaa aaaccatctc cagctcatag
     1741 ctaaaatccc cgaaacgcct gtaagtatcc aggctgatgg aagatatttg tggagggtcc
     1801 tggagaatat attctccaat gcggtcaaat attccatgag cgggacacgg gtgtacgctg
     1861 atctgacggc cgccggtaca gccgcaggca gtacggctgg cggcgatatg gctgtgggcg
     1921 gcatgcatgt gttcacattg aagaacattt caaaggaccc attgaacatt tctgccgacg
     1981 aactcaccga gcagttcgta aggggcgacc gcgcccgtta cactgagggc aacgggcttg
     2041 ggctttatat tgcgaaaagc ctgaccgagg ccatgggcgg ccggttcagc ataaatataa
     2101 acggcgacct gtttgaagtg cagatagcat tcaaatctgc ctgacatact taaccgaaaa
     2161 tccatcaaga ggatcacata tgcggctgct tatcaatgga ccggattttc tgtcaacttc
     2221 ctataatcgt ccaacacagc cgaaagcaga ccaataaagc tctttatttc ttcatccatg
     2281 ataaggatcc gaaatacttt tgttacattc cttttgaatc ttctgtcgat tttcagacca
     2341 aatgactcca ttaaaagcgg gcataaatat ttcgccctgg ggtcttctat aacagtaaga
     2401 ctcccgctct catcaatata cggagcgtag ggaaatatcc tgcatgccag gggtctgaac
     2461 cttctgttgc atttgccgct gcagacagca aaaagaacat ccgtatccct cattttcgtc
     2521 ttttgaatat tcaaaaaatc atcatggcct tccaatagtg attcctcacc cgggtagagg
     2581 cacattcccg cttcattgtc cccggaacag catttcctgc tgcaaataag gccgcagtca
     2641 aacttcagcg gggttgaatc cttcagcagt ctatagactt ttttatataa tatcttctta
     2701 tacactgtat tacctctcaa acggctctgc gtggcagtgc atttaccgaa tacatgactg
     2761 ccgcattaat gatttagcac cattatatcc tcactgcgaa atgattgtct ataaaatcaa
     2821 actcctgttg agcttcaccg catatgtact tactttatgt acaactggaa aaatccggat
     2881 aatcttatgc gttacataaa taacgttaca gaactaaagg atagtgcgtc cgtagaggct
     2941 ttgtacaaca gcatcatcaa ctttaacaat tggtatactt ccgacaggca gagcttttga
     3001 tgagtatatc cataggatat gcctcattgc ttttaagtta ttacggtacc catgtgtgtg
     3061 aaaattttgc atgccggtgt ggctaatctg cctgtatgcg tggtaaactt aatttgtgcc
     3121 ctaagcactt aaatactaaa catcatcgga ggtatgagcg ctatgaaaag gatgaacatc
     3181 gacagaggat ggtatctgga cacgaagcag tcagggttca atgattttgt caacgtcagg
     3241 aacatgcgtc aggtagatct tccgcatgat ctgatgctgg aatcggaagt ttcgcccgat
     3301 gttgaaggcg gcagggcaag cggatattac aagggcggtc ttgcctatta cacgaaattc
     3361 atcacgatcc ccgaagaatt gaaggaccag cgtatacttg ttgagataga cggatcgtac
     3421 atgaatactg aagtctattg cgacggcaat ttcgtcaaac gtcaccccta cggatatacg
     3481 ccttttcatg ccgacataac tccatatgtc aaattcggcc gggaaacgag gctgcaggtc
     3541 atcgtcaaca atccgttttc tttcacaagc cgttggtata caggcaccgg catttaccga
     3601 catgtcgata ttttgactgc gccgtatgta cacctttctc catggccgat ttttgcccat
     3661 acggatcgag tggacggcgg tacggcctat atcactgttg aagtgactgc ggagaaccat
     3721 actgccaaac cgggaatctg gcatatcgat gtagaactct gcaccgatga tgaaggcacc
     3781 gttgccgcga cagggcgcat ggcggtacag atacctgcca tggggaaacg tacgggccgg
     3841 gtcgggatag tggttgaaga tgcccgcctg tgggacatcg accatccaaa cctgtataag
     3901 gtaaaggcaa ttatgagaga attttcggag acacgcgacg atacacaggt gatagatgct
     3961 gcagaaactc tctttgggat caggaccata tccgtagata ccaaaaacgg attcatgctg
     4021 aacggccgtt cactgaaatt aaagggaggc tgtgtccacc acacgaacgg catacttggg
     4081 gcgacttccc tttacgactg tgaatacagg agggtaaagc ttcacaagga caatggcttc
     4141 aatgcgatac gctgcgcgca taatccgccg tcacgggact tcctggaggc ctgcgacagg
     4201 ctggggataa tggtcataga cgaagcattc gacatgttcc ggatgtcggg caacccgaat
     4261 gactaccacg cttatttcga aactcactgg aaatacgatc ttgaacagtt tatcacaagg
     4321 gaccgcaacc atccgtgcat tataatgtgg tcggcgttaa atgaagtgca ggaacggttc
     4381 gggctttccg acggctacgc ggtgtgcgcc gatattgccg actatatccg gaagcttgac
     4441 agtacgaggc ctgtaacagg cgcggtatgt atacctttca atggcctggc cgatgatgat
     4501 gcggcaaagc tgtacaaatc gtgggaagaa atggacgagt tgaagaagca gggtaacgca
     4561 acgcttcaga atgcatatgt gccgttcgga gacaagatat ggggatcgag gaccgaaccg
     4621 tttgtcgctc cgcttgacgt agtcgggtat aactatctcg actatcggta taaatcggat
     4681 catgagctgt acccgaacag gatcatagtc ggcacggaaa gcttcccgaa agatatcgca
     4741 gctacctgga agcgggtgga ggaatgtcct cacgtcatag gcgattttac ctggacaagc
     4801 atggattata taggtg
//
LOCUS       DAOSCC010000189         4805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11152_length_4805_cov_3.798526, whole genome shotgun sequence.
ACCESSION   DAOSCC010000189 DAOSCC010000000
VERSION     DAOSCC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11152_length_4805_cov_3.798526"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1804
                     /locus_tag="PLG72_09315"
     CDS             35..1804
                     /locus_tag="PLG72_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ81032.1"
                     /translation="MDYKEGIVQLISGPVVKGINMGAFMLNEMVTVGSKRLIGEVVSI
                     EGDVATVQVYEETEGLKAGEKIISTGSPLSVTLGPGMLGNIFDGIQRPLKKINSMSDG
                     FIPEGIGLLSLDEERKWPAHITVKPGDELRPGMVYATVQETLSIVHRLTVPAGTGGKV
                     IDAVPDGDYTIKDTVAVLEREDGSRAELKLSSKWPVRQPRPVAKRLPIYKPLVTGQRV
                     IDSFYPIGKGGTVGIPGGFGTGKTMTQHQIAKWCDADIIIYVGCGERGNEMTDVLEEF
                     PALTDPRTGRPLMERVILIANTSNMPVAAREASIYTGITMAEYYRDMGYDVALMADST
                     SRWAEALREISGRLEEMPAEEGYPAYLPSRLAEFYERAGCVRTLGGQEASVTIIGAVS
                     PPGGDFSEPVTENTKRFVTAFLALDKKLAYSRHYPAINYLTSYSGYITMLQDWYSENV
                     APDMLTLRAKMIRLLQEEEKLNEIVQLVGEDVLPNDQALVMEIARIIKKAFLQQNALH
                     KEDTYTVPAKQYKMLKVIDTLYESALKCVKLGIPISTIKSQDIVQDVLRMKENIPNDR
                     PELLDELCGRIVQVYSELSLKYE"
     gene            1841..3226
                     /locus_tag="PLG72_09320"
     CDS             1841..3226
                     /locus_tag="PLG72_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ81033.1"
                     /translation="MRVEYKKVVRVEGPLMVLSNVKDAAYGEMVSIRLDNDIVRKGKI
                     IKIDGTNAIIQVFEGTAGISTRNAAVEFKGEPMSIPLSREILGRTFNGAGEPIDGLYQ
                     VVSPERYNINGRPINPVARKYPRNFIQTGISSIDALMTLIRGQKLPIFSGSGISHNEL
                     AAQIVKQARIEGAGSDNFAVVFGAMGIRHDDANFFIKSFQASGVMDHVVMYINTADAP
                     IVERISTPRCALTAAEYLAFKLDMHVLVILTDMTSYAEALREISSAKGEVPSRKGYPG
                     YLYSDLSTIYERAGMIEGKEGSITLIPILTMPNDDITHPVPDLTGFITEGQIVLNREL
                     NLKGIYPPVDILPSLSRLMKDGIGADYTREDHADLASQLFAAYSRVQEVRSLAQIIGE
                     EDMNDIDRLYLQFGRELENRFISQGANENRSIRETLDLGWEILSILPEEELDRVNADI
                     LKKYYRKGSDN"
     gene            3229..3846
                     /locus_tag="PLG72_09325"
     CDS             3229..3846
                     /locus_tag="PLG72_09325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ81034.1"
                     /translation="MAMMVAPTKSGLIKAKSSLALSNKGFDLLDKKRNVLIREMMELA
                     DKSRNIQEKVYSTIAEAYQALQEANITMGLRDVDDITNSIPADEDFEILLKSVMGAEI
                     PSVRYKRKEIKPAYGFYNSNISLDIARQKFNEVRYCIYELAELENSIFKLAKEIEKTN
                     KRTNALEHIQIPKYSEQVKRIQEVLEEKEREDYFRLKWCQARQKQ"
     gene            4063..>4805
                     /locus_tag="PLG72_09330"
     CDS             4063..>4805
                     /locus_tag="PLG72_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOJ81035.1"
                     /translation="MSKNDVVKITLEESEIPRQWYNIVADMPNKPAPYHNPVTGELLK
                     PDDMKAIFPDALIAQEVSTERYIEIPDEVRELYRQWRPSPLYRARNLEKLLDTPARIY
                     YKYEGCNATGSHKLNSALAQAYYNKKAGIKRLSTETGAGQWGSALSMACNLFGLECTV
                     YMVKVSYQQKPYRRSFMETFGAKVHASPTNLTQSGRRILEEDPESTGSLGIAISEAVE
                     DAATHDDTNYSLGSVLNHVCLHQTVIGQEA"
BASE COUNT         1360 a         1088 c         1366 g          991 t
ORIGIN      
        1 acattgttcc aggctattga aacgggtgat cagtatggat tataaggaag gcatcgtaca
       61 gctaatcagc gggcctgtgg taaaaggcat caatatggga gcgttcatgc tcaacgaaat
      121 ggtcaccgtg ggaagcaaaa ggctgatcgg agaggtcgtt tcgatagaag gcgatgtcgc
      181 cactgtccag gtatacgagg aaacagaagg cctgaaagcc ggggaaaaga taataagcac
      241 cggtagtccg ctgtccgtga ctttgggacc gggtatgctg ggcaatattt tcgacgggat
      301 ccaaagaccg cttaagaaga tcaacagcat gtccgacggt ttcatacccg aaggcatagg
      361 actgctgtcg ctcgatgagg aaaggaaatg gccggcgcat ataacagtaa agccggggga
      421 cgagttgagg cccggcatgg tctatgccac ggtacaggag acgctgagca tagtccacag
      481 gctgacagtg cctgcgggta ccggcgggaa ggtgatcgat gccgtgccgg acggcgacta
      541 tacgataaaa gatactgttg ccgttcttga aagggaggac ggtagcaggg ctgaactgaa
      601 gctttcaagc aaatggccgg taaggcagcc caggcccgtt gcgaaaaggc tgcccatata
      661 caagccgctt gtgacaggac agagggtgat cgacagcttc tatcccatcg gcaaaggcgg
      721 cactgtcggg ataccgggcg gattcggcac gggcaagacc atgacccagc accagattgc
      781 caagtggtgc gatgcagata ttatcatata tgtcggctgc ggtgaacgcg ggaatgagat
      841 gacggacgtt cttgaggagt ttcccgccct tacggatcca cggaccggga ggccgctcat
      901 ggaaagagta atacttattg cgaatacatc caacatgccg gtcgcagcgc gtgaagcaag
      961 tatttacact ggcatcacca tggcggagta ttacagggat atgggctatg acgtggccct
     1021 gatggccgat tccacatcaa gatgggcgga ggcgctcaga gagatatcag gccgtcttga
     1081 agagatgccc gccgaagaag ggtatcccgc ataccttcct tccaggctgg ccgagttcta
     1141 tgaaagagcc ggatgtgtgc gcacgctcgg agggcaggaa gcgtcggtca ccatcatcgg
     1201 agcggtttcg cctcccggag gagacttttc cgaacctgta acggaaaaca caaaaaggtt
     1261 tgtaacggcc ttcctggcgc tggacaaaaa gcttgcctat tcgagacatt atccggcaat
     1321 aaattatctg accagctaca gcgggtatat tacgatgctc caggactggt actccgagaa
     1381 tgtggcgcct gatatgctta cgctcagggc aaagatgata agactgctgc aggaggaaga
     1441 gaaactgaac gaaatagtgc agcttgtcgg tgaggatgtg ctgcccaacg accaggcact
     1501 ggtgatggag atcgcaagga taatcaaaaa ggcattcctg cagcaaaatg cgctgcacaa
     1561 agaggacacg tatactgtac cggccaagca gtacaagatg ctgaaggtca tagataccct
     1621 gtacgagagc gctttgaagt gtgtgaagct gggaataccg atttcaacga tcaaaagcca
     1681 ggacatagtg caggatgtgc tgaggatgaa ggaaaacatc cccaatgaca ggccggaact
     1741 gcttgatgaa ctgtgcggca ggatcgtgca ggtatacagt gaactgagcc tgaagtatga
     1801 gtaaggccat aatcaatcca ttaatgttcg aggtcgagac atgagagtag agtataaaaa
     1861 ggttgtaagg gtggaaggcc cgctgatggt gctttccaat gtaaaagacg ccgcatatgg
     1921 cgaaatggta tctatcagac ttgacaacga tatcgtcaga aagggaaaga taataaagat
     1981 cgacggaacg aatgccataa tacaggtatt tgaaggtacg gcgggcatat cgacgagaaa
     2041 cgctgccgtg gaattcaaag gcgagcccat gtcgataccg ctgtccaggg agatactcgg
     2101 aaggacattc aatggggcgg gcgagcccat tgacggcctt taccaggtcg tttcgcccga
     2161 aagatataat ataaacggac gcccaataaa ccctgtggca aggaaatacc cgagaaactt
     2221 catacagacg ggcatttcct cgatcgatgc cctaatgacg cttattaggg gacagaaact
     2281 gcctattttc tcggggagcg gcatttccca taacgaactg gctgcgcaga tagtaaagca
     2341 ggcgaggatc gaaggggccg gcagcgacaa ttttgccgtt gtcttcggag ccatgggtat
     2401 caggcatgat gatgcgaatt tctttataaa aagcttccag gcctcaggcg tgatggatca
     2461 tgtagtaatg tatatcaata ctgccgatgc gcccatagtg gagaggatat ccactcccag
     2521 gtgcgcgctc acggcagccg aatatcttgc tttcaagctg gatatgcatg tacttgtgat
     2581 acttaccgac atgacgagct atgcggaggc gcttcgggaa atatcgtccg caaagggcga
     2641 ggtgccttcg cgcaagggtt acccgggcta tttgtacagc gatctttcga cgatatacga
     2701 aagggccggg atgatcgaag gcaaggaggg atccataacc ctcataccca tacttaccat
     2761 gccaaacgac gatataacgc accctgtccc ggaccttaca ggctttatca cggaagggca
     2821 gatcgtgctt aaccgtgaac ttaacctgaa gggtatatat cccccggtcg acatacttcc
     2881 gtcgctgtca cggcttatga aggacggtat cggtgcggac tatacaaggg aagaccatgc
     2941 agacctggca agccagctgt ttgcggctta ttcccgggtg caggaggtca gatcgctggc
     3001 tcagataata ggcgaggagg atatgaacga tatagacagg ctgtatctgc aattcggcag
     3061 ggaattggag aacaggttca tatcgcaggg cgccaatgaa aaccggagca tcagggaaac
     3121 actggacctt ggctgggaga tcctgtccat acttcctgaa gaagagctgg atcgtgtgaa
     3181 cgccgacata ctgaaaaagt attacaggaa aggcagtgat aactgattat ggccatgatg
     3241 gtggctccga ccaagtccgg cctgattaag gcaaaatcct ctctggcgct gtcgaacaag
     3301 ggctttgatc tgcttgacaa aaaaaggaac gtgcttatca gggaaatgat ggaactggcg
     3361 gacaagtcaa ggaatatcca ggagaaggtg tattcaacga tagcggaagc ataccaggcg
     3421 ctccaggagg ccaacataac aatgggcctg cgggatgtgg acgacatcac gaacagcata
     3481 cctgcggacg aggatttcga gatcctgctc aagagcgtga tgggggcgga aatcccttca
     3541 gtcaggtata aaaggaaaga aatcaagcct gcctatggct tctataattc aaatatatct
     3601 ctggatattg cacggcagaa gttcaatgag gtacgctact gcatatatga actggcggag
     3661 cttgaaaact ccattttcaa gctggcgaaa gagatagaga agaccaacaa gaggacaaac
     3721 gccctggagc atatccagat ccccaaatac agcgagcagg tcaagcgcat ccaggaggtg
     3781 cttgaggaaa aggagaggga agattacttc cgcctgaaat ggtgccaggc gcggcagaag
     3841 caataggact taagaacttt tccgccttct tcaggcattg cgcggatctt tgttcagcgg
     3901 cttactgtca tactccggcc aaactcccga atccggttcc ggacaaaaaa tcggaggtgg
     3961 gatggaggca ttttttcatt ctgtattgca attggttaca ttatgtagta aaataagtta
     4021 aaaatttaat aagacgagga gagatgagtt gagatgagca agctgagcaa aaatgatgtt
     4081 gtcaagatca ctttggaaga gtctgagatc ccaaggcaat ggtacaatat cgttgccgac
     4141 atgcctaaca aaccagcccc gtatcacaat ccggttacag gtgaattgct gaagcccgat
     4201 gacatgaagg ctatttttcc tgatgccctt atagcccagg aagtatctac tgaaaggtat
     4261 atcgagatac cggatgaggt ccgcgagtta tacaggcaat ggaggccgtc gcccctgtac
     4321 agggcaagga acctcgagaa gctgctggac actccggccc ggatatatta caagtatgag
     4381 ggctgcaacg caacaggaag ccacaagctg aattctgccc ttgcacaggc ttactacaac
     4441 aagaaagccg gaataaagcg cctttccacg gaaactggcg ccgggcagtg gggcagcgca
     4501 ctgagcatgg catgcaatct ttttggcctt gagtgcactg tttacatggt caaggtaagt
     4561 tatcagcaga aaccgtaccg gcgttcattt atggaaacat tcggcgcgaa agttcatgcg
     4621 agcccgacta acctgactca atccggaaga aggatccttg aggaggatcc ggaatcaaca
     4681 ggcagccttg gtatcgctat aagcgaagct gttgaggatg cggctaccca cgatgatacc
     4741 aactactcgc ttggcagcgt cctgaaccat gtatgcctgc atcagacggt catcggacag
     4801 gaagc
//
LOCUS       DAOSCC010000190         4790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11196_length_4790_cov_4.722070, whole genome shotgun sequence.
ACCESSION   DAOSCC010000190 DAOSCC010000000
VERSION     DAOSCC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11196_length_4790_cov_4.722070"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLG72_09335"
     CDS             complement(<1..249)
                     /locus_tag="PLG72_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOJ81036.1"
                     /translation="MLKKTLLLGLSVLLFLSAVMTVPAFGQIEGVVEYDSVEVMKIQN
                     NTSLTDLKAKAAILMDCSTGTVLLEKNSHEKLPIASITK"
     gene            complement(395..1714)
                     /locus_tag="PLG72_09340"
     CDS             complement(395..1714)
                     /locus_tag="PLG72_09340"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOJ81037.1"
                     /translation="MRITDIIGKKRDGKELSTEEINFFIEGICSGSIYDYQASALLMA
                     ICIRGLNSRETADLTAAMARSGETIDLLGIPGIKVDKHSTGGVGDKTTLVVGPIVAAC
                     GVPVAKMSGRGLGHTGGTIDKLESIKGFRTSLSREEFISNVKKIGIAISGQTGNLAPA
                     DKILYSLRDVTATISSVPLIASSVMSKKLASGADRIVLDVKTGSGAFMKTLEDSIELA
                     MEMVSIGELTGRKTVAVVTDMDTPLGNCIGNALEVKEAIEVLKGNGPEDLRTVCFELA
                     ARMLELGGKGDIAACRELVGKSINSGSALKKFGELAERQGAVYEPWDEKAMQEAIVRH
                     PVTSPCEGYIYSMKSELVGKASMLLGAGRETKDGQLDHSAGMILYKKTGEYVKKGEMI
                     AELHTSSRHLLPEAEAMLIQAYDISSEAPQTRPLILAYVDSSGVVRY"
     gene            complement(1851..3023)
                     /locus_tag="PLG72_09345"
     CDS             complement(1851..3023)
                     /locus_tag="PLG72_09345"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOJ81038.1"
                     /translation="MKRVIIIVLDSAGVGELPDAAEYGDEGSNTLGNIAAAVSGFSLP
                     NLEKLGLGNIDGIVGYKAADEPLGCFGKMAERSAGKDTTTGHWELAGITLRKPFPVYP
                     EGFPGDVIRQFEKEIGTKTLGNYPASGTVIINELGHQHVRTGYPIIYTSADSVFQIAA
                     HEDVIPVERLYEICRIARRILTGEHAVGRVIARPFVGTEGNYTRTANRKDFSLEPPGR
                     TLLDHVKEAGLEVKAVGKIEDIFAGRGITESVHIQDNMDGVDKTIGFIRDEFSGLIFA
                     NLVDFDMKFGHRNDAEGYAGALKEFDDRVPEIIDAMKEDDILFITADHGCDPTTASTD
                     HSREYVPLLVYGKQIRRSVDLGIRSTFSDLAQTVAEILGVSGSFDAESFLDRITKK"
     gene            complement(3706..4590)
                     /gene="xerD"
                     /locus_tag="PLG72_09350"
     CDS             complement(3706..4590)
                     /gene="xerD"
                     /locus_tag="PLG72_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOJ81039.1"
                     /translation="MEALVQEFVDFLEKDKRLSLNTLQSYRRDIEQYFSYLHEINLHN
                     IANTNKTTVITYLLYLQKKGRATSTISRNLASIRSFYQYISKCKLIDHDPTAELESPK
                     VEKKLPQILSPEEVELLLEQPQCVDLKGYRDKAMLELLYATGIRVSELIQLDIDDLNI
                     DMAYIRCCKGTRERMIPIGSMAINALKEYLSKSRPLLVQNEDEKALFVNINGSRLTRQ
                     GFWKIIKQYKNQAKISKDITPHTLRHSFAAHLLENGADLRSIQEMLGHSDISSTQVYA
                     QIAKNRIKEIYKKTHPRA"
BASE COUNT         1057 a         1305 c         1025 g         1403 t
ORIGIN      
        1 tttagttatg ctggctatcg gaagcttttc atggctgttt ttctccagca ggaccgttcc
       61 ggtgctgcag tccatcaata tggccgcttt tgccttcaga tccgtcaacg atgtattgtt
      121 ctgtattttc atcacttcta ccgaatcata ttcaactacg ccttcgatct gtccaaatgc
      181 gggcaccgtc attacggctg aaaggaacag caggacagac aggcctaaca acaaggtttt
      241 tttcaacatg atatacaccc cttgcgagca tacttcttac ccttatatta aagttatgcc
      301 gcatcagggc cgtatattct gtgaacttgt caggtatttg cctgtcagtt cctccgcgga
      361 tactaccgat tatgggatca tgaccgtttt aatttcagta cctgactact ccgctgctgt
      421 caacatacgc aaggatcagc ggtctggtct gcggagcctc ggaagaaata tcatatgcct
      481 gtatcagcat tgcttcagct tcaggcagaa ggtggcgtga cgatgtgtgg agttctgcta
      541 tcatttctcc ttttttaaca tattcgcctg tcttcttata aaggatcatc cccgcactgt
      601 ggtccagctg gccgtccttc gtttcgcggc cggctcccag cagcatggac gctttgccga
      661 cgagttctga tttcatgcta tatatgtaac cttcgcaggg cgatgttaca gggtgtctga
      721 caatggcttc ctgcatcgct ttttcatccc acggctcgta tacggcgccc tgcctttccg
      781 caagctctcc gaactttttt agcgcactgc cgctattgat actctttccc acaagttccc
      841 tgcatgcagc gatatcgccc ttgcctccaa gctcaagcat cctggctgcc aactcgaaac
      901 acacggtcct caagtcttcc ggtccgttcc ctttcagtac ttctatggct tccttcacct
      961 caagggcatt gccgatgcag ttgccaagcg gtgtgtccat gtcagttacg acagccaccg
     1021 tttttcttcc ggtaagttct ccgatggata ccatttccat ggccagttct atggaatcct
     1081 ccagcgtttt cataaatgct ccgctgccgg ttttcacatc gagcactatc ctgtcagccc
     1141 ccgacgccag cttcttgctc atcacgctgc ttgctatcag cgggacgctg ctgatcgtgg
     1201 cagtcacatc cctcagagag taaagtatct tatctgccgg ggcgagattc cctgtctggc
     1261 cggatatggc tatgcctatt ttctttacat tgctgatgaa ttcttctctc gaaagagacg
     1321 tccggaatcc ctttatagac tcgagcttgt ctatagtgcc gccggtatgg cccagtcccc
     1381 tgcccgacat ttttgccaca ggtacaccgc acgcggcaac gatggggccg accacaaggg
     1441 tagtcttgtc gccgacgccc cccgtgctgt gtttgtctac ttttatgcca gggatcccca
     1501 aaaggtcgat cgtctcgccc gatctggcca tcgctgccgt aagatcggct gtttcccttg
     1561 aattgaggcc tcttatgcaa atagccatca gaagggcgga agcctggtaa tcgtatatgc
     1621 ttccgctgca tatcccttct atgaaaaagt ttatctcctc ggtggacagt tcctttccgt
     1681 ctctcttttt cccgattata tccgtaatcc tcataaaacc acccgtttca ccatttatta
     1741 cataccgaca gtccgatatc atctttctgc ggaaagcgtc aggtcaactt cagccgatcc
     1801 agtcatgaga ggcagtcagc atttcggccg cggcaggcac aaaaaaccaa tcacttcttc
     1861 gttatcctgt ccaggaaact ctccgcatca aaactgccgc tgactccaag gatttccgcc
     1921 actgtctgcg ccagatcgga aaatgtactc ctgatcccaa gatccacgct tctcctgatt
     1981 tgcttgccat acaccagcag cggtacatat tcacgggaat ggtcggtgct tgccgttgtc
     2041 gggtcacagc cgtggtccgc cgtaataaaa agtatgtcat cctctttcat tgcatcgatg
     2101 atttccggca ccctgtcgtc aaattccttc aaagcaccgg catatccttc cgcatcgttc
     2161 ctgtggccga acttcatgtc gaaatccacg agattcgcga atatcagtcc gctgaattca
     2221 tccctgataa atccgatcgt cttgtcgaca ccgtccatgt tgtcctgtat atggaccgat
     2281 tccgttattc ccctgcctgc gaatatatcc tctattttgc ccaccgcctt tacttcaagt
     2341 ccggcttcct tcacgtgatc gagcagtgtt ctccccggcg gttccagcga gaagtccttc
     2401 ctgttggcag tcctcgtgta attgccttcc gttccgacga acggccttgc tatcacgcgc
     2461 cctacggcat gttcgcctgt aaggatcctc cttgcaatcc tgcatatctc atacagtctc
     2521 tctaccggga taacgtcttc atgagctgct atttgaaaaa cgctgtcagc cgaggtgtat
     2581 ataatgggat atcctgtcct gacatgctga tgtcctaact catttataat aactgttccg
     2641 gaagcaggat aatttccaag cgtctttgtc cctatctcct tttcaaactg ccgtataacg
     2701 tccccaggaa atccttcggg gtacaccggg aaaggtttcc tgagagtgat gccggcaagt
     2761 tcccaatggc ctgtcgtggt atcttttcct gcggaacgtt cagccatctt tccaaagcat
     2821 cccaaaggct catctgctgc tttatagcca actataccat caatgtttcc aagtccaagc
     2881 ttctcaaggt tcggaagtga aaaaccgctt accgccgcag cgatattgcc aagcgtatta
     2941 cttccttcgt ccccatactc cgcggcatcc ggcagttcgc cgacacctgc gctgtccagt
     3001 acaatgatta tgactctctt cataccgttc accttcattc cggcagttgc ttatctgccg
     3061 gttctttcgt actgctatgg tttctataac aaattgtacc acattatttt attttgaata
     3121 aacggaatca tgattcgaat ggaattatgg ccttcctggt ccgggattga gccgggatga
     3181 cggcttgtca atacattgat gggctgcaag gtttattgtt tctcattact taagtgaacc
     3241 ggagggatgg ccggtcaagt cattactggg ctgcaatttc tgtatattcc ctggaaccgg
     3301 aatgaatcgg gaggatgggc ggtcaattta tcgatgggtt gcaagttata cagatacgac
     3361 cgggtcatgc agctcgaatg aggcatcagg caccacaacc ggcagcacgg acgctgccgg
     3421 cgctggtccc gagccacgga cggcgagtga cctgcgccct cattggagca aatgagccgg
     3481 tacgtagatg ttaacgcagc aaccccaaat agattgaccg cccatcctcc cgatttactc
     3541 aaatacagga aattaaaaat tgcagcccat cggtggcctg accgaccggg ttccggcctg
     3601 tcccgggcac aggaagccgc ctgttgacat tatcctcagc atataaataa aaaaacctgc
     3661 tgtcagcagg ttttatgatt cattacagtt ctgtgcgcct ccatatcacg ctctcggatg
     3721 cgtcttcttg tatatctcct tgatcctgtt cttggctatc tgggcgtaca cctgggtgga
     3781 ggaaatgtcc gaatgcccga gcatctcctg tatcgatctg agatccgcgc cgttctcaag
     3841 caagtgcgcc gcaaatgaat ggcgcagagt atggggcgtt atgtccttgc ttattttcgc
     3901 ctggtttttg tattgcttta ttatcttcca gaagccctgc cttgtcagcc tgcttccgtt
     3961 tatattgacg aacagggctt tctcatcttc attctgtacc aacaggggcc tcgatttgct
     4021 cagatactct ttcagcgcgt ttatagccat ggaaccgatg ggtatcatcc tctcccttgt
     4081 gcccttgcag catctgatat aagccatgtc tatgttcaga tcgtcaatat cgagctgtat
     4141 cagttccgac accctgatac ccgtagcata cagcagctcc agcattgctt tgtcccgata
     4201 gcccttgagg tcgacacact gcggctgttc cagcagaagc tccacttctt ccggagataa
     4261 tatctgcgga agctttttct cgactttcgg cgactccagt tccgctgtcg gatcgtgatc
     4321 gatcagtttg cacttggata tgtattggta aaaagacctg atcgatgcca ggttgcgcga
     4381 aatagtcgat gtagcccgtc cctttttctg gagataaagc aggtatgtga tgaccgtagt
     4441 cttgttcgta ttggcaatat tatgcaggtt tatctcgtgc aaatatgaga aatactgctc
     4501 aatatcccgt cggtacgact gcagagtatt cagcgacaac cttttatcct tttccagaaa
     4561 atcaacaaac tcctgtacta aagcttccat ccgtactacc cctttatatt taattgttgt
     4621 ttactaccgt tgttatttat tgattgtaat atatttcaat accaaatgaa agaaattttt
     4681 gctattatat agcattctta ctaatcatgc aataaatatt acttaagaat tccttatttt
     4741 gcaataaata tttgtttatg taaatattat ataccgtttt ctctatatta
//
LOCUS       DAOSCC010000191         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11678_length_4645_cov_4.624619, whole genome shotgun sequence.
ACCESSION   DAOSCC010000191 DAOSCC010000000
VERSION     DAOSCC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11678_length_4645_cov_4.624619"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    134..430
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            463..1293
                     /gene="tyrS"
                     /locus_tag="PLG72_09355"
     CDS             463..1293
                     /gene="tyrS"
                     /locus_tag="PLG72_09355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ81040.1"
                     /translation="MTGRLYRPAELEHTGLKTVLDFDALCINTRLEANEPIGIHEFLY
                     PLMQGYDSVVVKADVELGATEQRFNVLMGRKLQKDFGQQSQVALFMPVLEGTDGVEKM
                     SKSLGNYIGINEDAANMYGKVMSIPDQLIIKYFRLVTDVHPDEIDAMEKALRNNEINP
                     RDLKMKLAFEITGLYHGNEAAVRAEENFVSVFQRKGIPGDITEYRLDSELITVGGVDM
                     AELLVRMGFSASKSEARRLITQGAVRINGEKLTGLTAAALDNGDIIQAGKLKFARILR
                     "
     gene            1458..1895
                     /gene="mscL"
                     /locus_tag="PLG72_09360"
     CDS             1458..1895
                     /gene="mscL"
                     /locus_tag="PLG72_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOJ81041.1"
                     /translation="MLKEFREFAMKGNVIDLAVGVIIGGAFGKIVTSLVNDIIMPLLT
                     FITKRGELSFENLFIALDGKHYETLAAAQEAGAPTFNYGLFLTNVLDFLIISFVIFMF
                     VKQINRLKRKPEPAPVATKECPYCRTAIHKDAVRCPNCTSELH"
     gene            complement(1974..3056)
                     /locus_tag="PLG72_09365"
     CDS             complement(1974..3056)
                     /locus_tag="PLG72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HOJ81042.1"
                     /translation="MDISFKSCKGTAAVTTALMMTLILGFSALVVDIGIVSIQKSRLQ
                     NAVDSAALAAAQDLPSTADAADTARAYLDSNGFESASINVSFANNDRQVTVEAAQSFD
                     YMLAGVFGMDSVEVMASATAQKGGRYLRGAFEYAIFSSDMNQELILNSNGKDTYVEGS
                     VHSNNGFRYNGNHDSLVITGVLDTVGRITLNSIHTDIGEQRPFNSYIEMPDFSEEIKQ
                     LALKQGTYYNTSQSFNSDLHVDEAIYVNGHINFNCNKFSGIGTIFAKDNINFNHSVVY
                     ESPGTDSICIYSQNGNIHFNSSGAVIRGTIYAPNGTVVLNNGLHTVYGRIIAQKIIFN
                     GGIEVYSSPGDLVFMEPFDEGIRLVR"
     gene            3358..3783
                     /locus_tag="PLG72_09370"
     CDS             3358..3783
                     /locus_tag="PLG72_09370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ81043.1"
                     /translation="MVVIKKVRTKDEMDQVYDIRREVFIEEQGVPEDIEMDGRDDEAV
                     HVLAVVDGEPAGCGRLLISGDEARIGRVAVRKKMRRCGIGDGICRLLIALAKDRGAGK
                     IVLDAQLTAEGFYSRLGFEREGDIFMEAGMEHVRMTMKI"
     gene            3960..4181
                     /locus_tag="PLG72_09375"
     CDS             3960..4181
                     /locus_tag="PLG72_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOJ81044.1"
                     /translation="MTLDEAKRGDQLQIVNIKDALVRSQAIRLGISEGSRMQCAEIVP
                     HGPIILKNRKQEVAIGYKLAHKIKVERVG"
     gene            4185..>4645
                     /locus_tag="PLG72_09380"
     CDS             4185..>4645
                     /locus_tag="PLG72_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOJ81045.1"
                     /translation="MSSQKPGKAGETHAADAPKLVLAGNPNVGKSVFFNALTGMYVDV
                     SNFPGTTVEISRGRYRDYDVIDTPGVYGVSSFNDEEKAARDIILEADLVLNVVDAVHL
                     HRDLFLTQQLIDMGKRVIIALNLMDEAEKNGIRVDVPKLSEMLGVPVIPTMA"
BASE COUNT         1216 a          994 c         1327 g         1108 t
ORIGIN      
        1 cttaaaacag ggttctgtaa gattttgtaa ggcaattaaa cttgcaattt aaatcaaaaa
       61 atacgttgac aattcgtatt gacagtgggt ataatagcga aaatacagtt taatattgaa
      121 aaagaatacc tgatcaaaag cattgatgga gaaggagtaa gggcaatgga tcccgacaga
      181 gagttcccgg gcggtgagag gggcggggag acttgttctg aatacatctc cggagctgca
      241 ggctgaaagt gcatataatg catgagtagg ctgtgccgga agcctgcgac cgttacagcg
      301 ctagtggata atgtgaccgg aaagggtttt gcctgcggca gcaatacggc acacgtccat
      361 aataaggtcg gtaccgcaag gtatcgatga aatgaggtgg taacacgaag cgagagctct
      421 cgtcctctga cagcattcag aggccgaggg cttttttggt ttatgaccgg ccggctttac
      481 cggccggcgg agctggagca taccggctta aaaactgtac tggattttga tgcgctgtgt
      541 atcaacaccc gtcttgaggc caatgagccc atcggcatac acgagttcct ctatcccctg
      601 atgcagggct acgattcagt ggtggtaaaa gcggatgtgg agcttggcgc gacagagcaa
      661 aggttcaatg tactgatggg caggaagctt cagaaggatt tcggacagca aagccaggtc
      721 gctttgttca tgcccgtact ggaagggacc gatggagttg agaaaatgag caagagcctg
      781 gggaattaca tcggcataaa tgaagacgcc gcaaacatgt acggcaaggt catgtcgata
      841 ccggaccagc tcataataaa gtatttcagg ctggtgacgg atgtccatcc cgacgagatc
      901 gatgccatgg aaaaggcgct cagaaacaat gaaataaatc caagggatct caaaatgaaa
      961 cttgcctttg agataaccgg gttatatcat ggaaatgagg cagcagtccg ggcggaagaa
     1021 aatttcgttt cggtgttcca aaggaaaggt atccctgggg atataacaga gtacaggctt
     1081 gacagtgaac tgatcacggt aggcggcgta gatatggcgg aactgctggt aaggatgggt
     1141 ttcagtgcga gcaaaagcga ggcaaggagg ctcattacac agggcgctgt caggataaac
     1201 ggagagaagc tcaccggtct gaccgctgca gcactggata acggagacat aatacaggcg
     1261 gggaagctca agtttgcgcg gatattgcgg taaggcagcc gtgaataaca tgcctggaca
     1321 gtaccggacg cttgccgatg ccgtacggga tatttgctgt cgcagtaccg gacgtttact
     1381 gatacggagc cggttgtttg agattcagcc ggatgctcat gctgccggta aaaataaaag
     1441 gagggtttga gatgaaactg cttaaggaat tcagggaatt tgcgatgaaa gggaatgtca
     1501 tcgaccttgc ggtcggcgtc attatcggcg gcgcgttcgg gaaaatagtc acatcacttg
     1561 taaacgacat cataatgccg ttgcttacgt ttatcacgaa gcgtggcgaa ctgagcttcg
     1621 aaaacctgtt catcgctctt gacggaaaac attatgaaac tctggccgcc gcgcaggaag
     1681 cgggagcgcc cactttcaac tatggcctgt tcctgacgaa cgtacttgac ttcctgatca
     1741 tatcattcgt cattttcatg ttcgtgaagc aaataaacag gttgaaaaga aagcctgaac
     1801 cggcgccggt ggcgactaag gaatgcccgt actgccgtac cgctatccac aaggacgctg
     1861 taaggtgccc gaactgcaca tcggaactcc attgatacct ggagttccga tgacaggtgg
     1921 ggggagttgg caagatgtcc ttacaggggc tttgtttata ttggggacag gctttatctt
     1981 accagcctga ttccttcgtc aaagggttcc atgaagacaa ggtctccagg tgaactgtaa
     2041 acttcgatac cgccgttgaa gatgatcttt tgcgctatta tccttccgta aaccgtgtgg
     2101 aggccgttat tgagaacgac ggtgccgttg ggcgcataaa tggtgccccg gatgaccgcg
     2161 ccggagctgt tgaagtgtat attgccgttc tgcgagtata tgcagatgga atccgtgccc
     2221 gggctttcgt atacgactga atggttgaaa ttgatattgt ctttggcgaa tattgtcccg
     2281 atgccggaga acttgttgca gttgaagttg atgtgcccgt ttacgtatat ggcttcatcg
     2341 acgtggagat cgctgttgaa gctctggctg gtgttgtaat aggtgccctg cttcagggcg
     2401 agctgcttta tttcttcgga aaaatcgggc atttcaatat agctgttgaa agggcgctgt
     2461 tccccgatgt ccgtatggat actgttgagc gtgatcctgc cgacagtgtc gagcacgcct
     2521 gtgataacca gcgagtcgtg gttgccgtta tagcggaaac cgttgttgga atgtacgctt
     2581 ccttccacat atgtatcctt gccgtttgag ttgaggatga gttcctggtt catgtcgctg
     2641 gagaatatgg catattcaaa cgcgcctctc aggtatctgc ctcccttttg ggccgttgct
     2701 gaagccatga cttcaacgga gtccatgccg aagacaccgg ccagcatgta gtcaaacgac
     2761 tgggctgctt ctacggtcac ctgcctgtca ttgttcgcga aacttacatt tattgatgcc
     2821 gattcgaaac cgttgctgtc caggtatgcc cgtgcagtat cggccgcgtc ggcggtggag
     2881 ggcaggtcct gtgctgcggc cagggcagct gagtcgacag cgttctggag cctgctcttc
     2941 tgtatgctta cgatgccgat gtcaacgaca agcgcactga agccaaggat cagcgtcatc
     3001 atcagggctg tcgtaacagc cgctgtccct ttgcagcttt taaaggaaat atccatgcaa
     3061 atacctcctg actttattct ataacctctt cagaataaaa aaaggtacgg cagtcacaac
     3121 atttatgggg aaaaagtact atttttgata cagtgatgag ccgaagcgcc tgtgttataa
     3181 tgtgagatgc tgatataatg ctgacgtgat acaaatgatc gccggcatgg tgccaatata
     3241 aagaccaacg gctggttttc cgggatcttg cagcccggtc acctgaacag tcggctgcaa
     3301 tgcctggaaa gcatgctttg gcggagatca agaatatgaa aggaaggaac atgatcgatg
     3361 gtggtaataa aaaaggtcag gacgaaggat gaaatggatc aggtgtatga tatcaggcgc
     3421 gaagtgttca tcgaagaaca gggagtgcct gaagatatag agatggacgg aagggacgat
     3481 gaggcggtcc atgtgctggc tgttgtcgat ggcgagcctg ccggctgcgg aaggcttttg
     3541 atcagcggcg atgaagccag gatcggaagg gtcgcggtca ggaaaaaaat gcgcaggtgc
     3601 ggcataggag acggcatatg cagactgctg atcgcacttg cgaaggacag gggcgcaggt
     3661 aagatagtcc ttgacgccca gcttactgcc gaaggattct attcacgtct tggtttcgaa
     3721 cgcgaaggcg atatcttcat ggaagcgggc atggaacatg tgaggatgac catgaaaatc
     3781 tgaactgttt gggaatattt gagccgtcct ggcgctcatg tgattattgc ccggttttca
     3841 tcttattttc cggtcatttt ttgttcattt ttcacatcta attgatattg acattcatta
     3901 tcaatagggc taaaatttag atagcaaatg ataatgatta tcaattacag gtgagatgta
     3961 tgacactgga tgaagcgaag cgcggagatc agttacaaat agtcaatatc aaggacgccc
     4021 tcgtacgttc acaggctata aggcttggca tatctgaggg ttccaggatg cagtgcgccg
     4081 aaatagttcc gcacggtccg ataatcctga agaacaggaa gcaggaagta gccataggct
     4141 ataagctggc gcacaaaata aaggtggaaa gggtaggata ggccatgtct tctcaaaaac
     4201 cggggaaagc cggagagacc catgcggcag acgctccgaa gcttgtactg gcaggcaacc
     4261 caaacgtggg aaagtctgtc ttttttaatg cccttacggg tatgtacgtg gatgtatcga
     4321 atttcccggg gaccactgtg gaaattagca gaggcaggta tcgggactac gacgtcatag
     4381 acactccggg agtctacggg gtatcatcct tcaatgacga ggaaaaggca gccagggaca
     4441 tcatcctcga ggctgacctt gttctgaacg tggtggacgc ggtacacctg caccgtgacc
     4501 tgttcctgac ccaacagctg atagacatgg gcaagagggt catcattgcg ctcaacctca
     4561 tggatgaagc cgagaagaac gggataaggg tcgatgtgcc gaagctttcg gaaatgcttg
     4621 gagttcccgt gatccctacg atggc
//
LOCUS       DAOSCC010000192         4636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11717_length_4636_cov_4.458852, whole genome shotgun sequence.
ACCESSION   DAOSCC010000192 DAOSCC010000000
VERSION     DAOSCC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11717_length_4636_cov_4.458852"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..898)
                     /locus_tag="PLG72_09385"
     CDS             complement(158..898)
                     /locus_tag="PLG72_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ81046.1"
                     /translation="MSIRVLIADDDEGMRLVLKKAVERSGGFVIAGEAEDGGAALRLF
                     ESERPEVVFLDVEMPVMSGIECAKKIADIDPRTVIIFATAHEDYMPEAFEVYAFDYLV
                     KPFRLQRLEQTLDRIKGIGRFKHEVYENKAARSANTLQKLMIRNKEGISLVDTREIIL
                     IQREDRTTAIYTAQDRFVTSDSLGDIEQKLDGSVFLRCHKSYIININYIDRIYPYGRW
                     TYIVKLKGTDKDALMTRESFEKLEQILG"
     gene            complement(895..1434)
                     /locus_tag="PLG72_09390"
     CDS             complement(895..1434)
                     /locus_tag="PLG72_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81047.1"
                     /translation="MKKVINNPFDPATKFGLSPDPSVLGILGQDRGNKHGVRWMPQNT
                     RGVKYGMPQKRHSAYNAPGFGGTGTDSMASGAGNMESAAGTAGNISVTETGSISAGKS
                     ERGTAATAKNTQTGTNTAQKTFRRSHCVSHGNVLSETVAQKADGLGLLFSKDDMLRGF
                     IMAEVLGRPKCLRRGGGMI"
     gene            complement(1452..2441)
                     /gene="floA"
                     /locus_tag="PLG72_09395"
     CDS             complement(1452..2441)
                     /gene="floA"
                     /locus_tag="PLG72_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616782.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOJ81048.1"
                     /translation="MEFLVPIVIVILVIIFLFLFFNFIPVGLWISAFAAGVRVGIFTL
                     VGMRLRRVVPSKIILPLIKARKAGLDLSVNQLEAHYLAGGNVDKVVNALIAAHRANMD
                     LQFTRAAAIDLAGRDVLEAVKMSVNPKVIETPNISAMAKDGIELLVKARVTVRANLER
                     LIGGAGEATIIARVGEGIVTTVGSSESHKDVLENPDLISQTVLDKGLDSGTAFEILSI
                     DIADVDVGRNIGAQLQISQAEADKNIAQAKAEERRAMAVAKEQEMKALVQEMRARVVE
                     AEAEVPRAMANALNSGKIGVMDYYNLQNLISDTRMRESISKIGMPEEEKSADE"
     gene            complement(2780..3289)
                     /locus_tag="PLG72_09400"
     CDS             complement(2780..3289)
                     /locus_tag="PLG72_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ81049.1"
                     /translation="MEFAAIFTGYNWIPLVCIFAGIIFIIIEMYQPGFGVPGVIGILL
                     VAAGIILYARTVLQALIMIAVVLAILGAALALVLQSASKGRLAKHIVLESTVDDDVKF
                     SAMDDLSYLIGSEGRTVTVLRPSGIADFNGVRLDVVSGGEYIPKGTIVYVDKIEGNKI
                     VVKQSPRTQ"
     gene            complement(3414..4292)
                     /locus_tag="PLG72_09405"
     CDS             complement(3414..4292)
                     /locus_tag="PLG72_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HOJ81050.1"
                     /translation="MKKRFEPGKTALLLILINLVQVALIGAVFLYLTEMGKGAELLSN
                     TWSLAFLAVMAVSVAMNSVIVFRSRAGSIRSWHEFRLLEDTLAKVERLNATLRAQRHD
                     FMNHLQVVHSLIEMDEYRSAAEYIEKVYDDIQRVSRVMKTSVAAVNALLQAKLMACEK
                     QGITMELDIRTQLKDLAIPSWEFCRVLGNLIDNAIYAVLEADGEKYIRIELSEDLRHH
                     YFRVMNSGRPIPAELTGKIFEAGFTTKGDKGEGMGLAITRKIVEENGGSIGVSREGDL
                     TVFGGHIPRQHAGIEN"
BASE COUNT         1001 a         1326 c         1050 g         1259 t
ORIGIN      
        1 ggaaatactg aacagcgatt cggagattta tggcggcagc aatgcgggca attttggcgg
       61 cgtccgggcg aatgcagaag gacatgacgg acagccttat tctgtcgata tttgtgtgcc
      121 tccgctggca gtcgtgatat tcaagccggt ccaccaatca ccctaaaatc tgctcgagct
      181 tctcgaagct ttcgcgggtc atcagcgcat ccttgtccgt ccctttcagc ttcacgatat
      241 atgtccatcg tccatagggg tagatcctgt cgatatagtt gatatttatt atgtatgact
      301 tgtggcatct gaggaatacg ctgccgtcca gcttttgctc gatatcgccc aggctgtcgg
      361 acgtcacgaa cctgtcctgg gccgtataga tcgccgttgt cctgtcttcc ctctgtatca
      421 ggattatctc cctggtatcg acaaggctta tgccttcctt gttcctgatc atcagctttt
      481 ggagggtgtt ggcggacctg gctgctttgt tctcataaac ttcatgtttg aacctgccga
      541 tgccttttat cctgtccagc gtctgctcaa ggcgctgcaa tctgaacggc ttcacgagat
      601 aatcgaaagc atatacctca aatgcctccg gcatataatc ctcatgtgcg gtcgcaaaaa
      661 tgatgacggt ccttggatct atgtctgcga tcttctttgc acattcgata cccgacatga
      721 ccggcatttc aacatcaaga aacacaactt ccggtcgctc ggactcaaaa aggcggagag
      781 cggcgccgcc gtcctccgct tcccccgcaa taacgaaacc tcctgacctc tccacggcct
      841 ttttcagaac aagccgcata ccttcatcat catcggctat aaggaccctg atgctcaaat
      901 catgccaccg cctcttctta ggcattttgg cctgccaagt acctcggcca tgatgaagcc
      961 tctcagcata tcatcttttg aaaaaagcag tcccaggcca tccgcttttt gggcaacagt
     1021 ttcgctcagg acattgccat ggctgacaca gtggcttctt ctaaaagttt tctgtgccgt
     1081 atttgtgccg gtttgtgtgt tcttggcagt cgctgcagtt ccacgctcac ttttccctgc
     1141 cgatatggaa cctgtttcag taacactgat attaccggca gttcctgcgg ctgattccat
     1201 atttcccgca cctgatgcca tactgtccgt tccagttccg ccaaacccgg gagcattata
     1261 ggcggaatgc cgtttctgcg gcatgccgta ctttacaccg cgtgtatttt ggggcatcca
     1321 ccggacaccg tgcttgttcc cccgatcctg tcccaggatc cccaaaacgc tcggatcagg
     1381 tgaaaggcca aatttcgtgg caggatcaaa tgggttgttt attactttct tcatatcttt
     1441 acctttctgt cctattcatc tgcggatttt tcctcttccg gcatgccgat cttcgagatc
     1501 gattctctca tgcgggtatc agatatcagg ttctgcaggt tgtaataatc catgacgcct
     1561 atttttccgc tgttgagcgc attggccatt gccctgggca cctctgcctc ggcttcgacg
     1621 actcttgccc gcatctcctg caccagcgct ttcatttcct gttctttggc gactgccatt
     1681 gcacgccttt cctcagcctt ggcctgggcg atgttcttgt ctgcttccgc ctgggatatc
     1741 tgcagctggg cgccaatatt cctgcctaca tccacgtccg ctatatcgat cgacaggatc
     1801 tcaaatgcag taccggaatc aaggccttta tcaagcactg tctgggatat caggtcaggg
     1861 ttttcgagca cgtctttgtg agattcggaa ctgccgacgg tggtgacgat accttcgcct
     1921 accctggcta tgatggtggc ctcgcctgct cctcctatca gtctttcaag gttagccctg
     1981 accgttacac gcgccttgac aagcaattct atgccgtcct tggccatagc agagatgttc
     2041 ggcgtttcta tcaccttggg gttgacactc attttcacag cttccagcac atccctgccc
     2101 gcaaggtcta tggcggcggc cctggtgaac tggaggtcca tattggccct gtgggcggca
     2161 atcagcgcat ttacgacctt atccacatta ccgcctgcca ggtagtgcgc ttcaagctgg
     2221 ttcacactta aatcgaggcc tgctttcctt gcttttatca acggcaatat gatcttgctc
     2281 ggaacgactc tcctgagcct cattccaaca agcgtgaata tgccgacccg tacgcctgcc
     2341 gcaaatgccg atatccacag tccgaccggg atgaagttga aaaagaggaa cagaaaaatg
     2401 atgactaaaa tcacgataac tatgggtacg agaaattcca taacatccga ctcctttcat
     2461 tttatatctg ccggtccgat cacaaaacag ctgccgactg gagctcaaaa ccgacttgat
     2521 gcaaattgtg cagcctgatc cataaaaatg cacttgcagc cagatcaatg catatatcta
     2581 ttcagtatac cttttatacg tcatgccgca gggttttgtc ttgtggccat accggcacca
     2641 ggcatttccc tgcgtttatc ctggcaccgg gcgcttcccg gcatcgaatg gttctgtgcg
     2701 atcaccgcag tatcacaatt ctccacgtcg ccgggatctg agtctccccg catcatcggg
     2761 attcggatcc gttgcttgat cactgcgtcc gcgggctttg tttgacaact atcttattgc
     2821 cttcgatttt atcgacgtat actatagtgc ccttgggaat atactctccg ccagaaacca
     2881 cgtccagcct taccccgttg aaatctgcta tgccggacgg cctgagtacc gtcaccgtcc
     2941 tgccctcgct tcctatcagg tagctgaggt catccattgc tgagaacttt acgtcatcat
     3001 caacggtgct ttccaggacg atatgcttcg caaggcggcc tttcgacgcc gactggagca
     3061 ccagcgcaag ggcggccccg agtattgcaa gcacgacggc tatcattatc agcgcctgga
     3121 gtactgtcct ggcataaagt atgattcccg ccgccaccag cagtattcct atgacgcccg
     3181 gcacgccaaa accaggctgg tacatctcta ttatgataaa aattatccct gcaaatatgc
     3241 acaccagcgg tatccagtta tagcccgtga aaatagcggc aaattccata ttgacacctc
     3301 ctgtcccgtc cattctccaa tctcgcaatt atactttaat acatcctttt cgcggattgg
     3361 atacaaaact tctgaaaaat acatattgac tgctgaattg tcacttagcc gtcttaattt
     3421 tcaataccgg catgctgcct ggggatatgt ccgccaaaca cagtcaggtc cccttccctg
     3481 gaaaccccga tgctgcctcc gttttcttcg acgatctttc tggttatggc gaggcccatc
     3541 ccttctccct tgtctccttt tgtcgtgaac cccgcttcga atatctttcc tgtcaactct
     3601 gcgggaatgg gcctgccgct gttcatcacc ctgaaatagt ggtgccgcag gtcctcagac
     3661 agttcgatcc tgatatattt ctcgccgtcc gcttccagaa cggcatatat ggcattgtcg
     3721 atgagatttc caaggaccct gcagaactcc catgacggga ttgccagatc cttcagctgc
     3781 gtcctgatat caagctccat ggtgatgccc tgcttttcgc aggccatcag ttttgcctgg
     3841 agaagcgcat tgaccgcagc gacagaagtc ttcattaccc tgcttacgcg ctggatatcg
     3901 tcatagactt tttctatgta ttcagctgcg gatctgtact cgtccatctc tataagcgag
     3961 tggacaacct ggagatggtt catgaaatcg tgcctctggg ctcgcaatgt ggcgttcagc
     4021 ctctccactt tcgccagtgt atcttccagc agcctgaatt catgccagga gcgtatcgac
     4081 ccggccctgc tcctgaaaac gatgacactg ttcattgcca cggagacagc cataacagca
     4141 agaaacgcaa ggctccacgt gtttgaaagc agttccgcgc ctttgcccat ttcggtgaga
     4201 tacaaaaaaa ccgcaccgat aagcgcaacc tgtacaaggt tgatcaatat caacaacagt
     4261 gcggttttgc caggttcaaa ccttttcttc acaggactcc acctttcaaa taaataccat
     4321 atccagtata tcataaatac ttatccgtgg catggttttc aggcaggaag cggttctccc
     4381 gaagggcatg gggcgttccc caggtcctct tcagataggg ggacggttcc tgtgcctggg
     4441 tcctgttccg taacccggat acgaggaccg tccccatgtc ccaggtaata aaaaaaagcc
     4501 tcactgcgtg aggctcgttt ttctgttcag tgatgttgaa acaggaggcc gaagaatgat
     4561 cttgcgtcct cctgcttcag gtggtgaccc ataggggact cgaacccctg ttaccgccgt
     4621 gaaagggcgg tgtctt
//
LOCUS       DAOSCC010000193         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11746_length_4624_cov_4.749617, whole genome shotgun sequence.
ACCESSION   DAOSCC010000193 DAOSCC010000000
VERSION     DAOSCC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_4624_cov_4.749617"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLG72_09410"
     CDS             complement(<1..1056)
                     /locus_tag="PLG72_09410"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOJ81051.1"
                     /translation="MRRVKVDSVPEGARLARTVFSSDGGVLLMQGVELRDSYLELLRK
                     RGIYEIYLDDDLSEGIEVNDVVNEKTRNEAVVLVRDIMTGYSFSDAIDVGHVKTIVNR
                     IVDELLSNDDILYNLTEIKTVDDYTFKHSVSVCILSIITGIGLGFDTAQLRELGLGAI
                     LHDIGKLCIPREILKKPSQLTVAEFEEIKKHTILGYELLKKSGQLNLISSYIALGHHE
                     RYDGSGYPYRLKSEEIQIYARIVAVADVYDALTSDRVYRKKLKPHEVYEYITSMGLHH
                     FDTVVVENFVRFVTVYPEGSGVLLNTRERAIVVKHNRKMPTKPLVRIVYDEDMKRTSR
                     EIDLSQETGIFIVDICEV"
     gene            1238..1951
                     /locus_tag="PLG72_09415"
     CDS             1238..1951
                     /locus_tag="PLG72_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81052.1"
                     /translation="MIRSIPGDIAKHGFLLIRIAVTARRILSKLLSFRPADMLSDDMR
                     YQLYFYRSYLPELYRRHVSKNYKNCLFCSRLVKSGCHRYCLKCSAGTYIKCYPPLPGL
                     LYAVPVHTDDGDQLAVPWYFRKTCSGFKRLPLFWYWHNIYSVFPSIALSNYEALEGLE
                     KGLSSGARPCHICATVDYELYRKCMKQPDFDVSAPCTKIREELKKAYRGQTAGKPEKS
                     RATSLKSQDPQYKHTETAV"
     gene            complement(2071..3816)
                     /locus_tag="PLG72_09420"
     CDS             complement(2071..3816)
                     /locus_tag="PLG72_09420"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOJ81053.1"
                     /translation="MNSREAFEFWMRSDYFDQETKKELEKISNDPDEIEERFYKDLEF
                     GTGGLRGIIGAGTNRMNIYTVRKASQGLANYLLNNVKDAAARGVVIAYDSRRMSPEFA
                     REAARVFAANGIVSYLFDELRPTPELSFAVRHLKAAAGVVVTASHNPKQYNGYKVYGE
                     DGGQLPPEGSEAVLKEINGITDITAIKPADEKEALGKGLIRMIGKEVDDAYIESLKTL
                     LVNPDEIKKAADSMKIVYTPLHGSGNKPVRRILAEAGFKNVLVVREQELPDPEFSTVK
                     YPNPEEKDAFRLAIELARKEDVDIIIGTDPDSDRVGVVVRNSAGEYAVLTGNQTGCLL
                     MEYILSQKKAAGCLPENGFVVKTIVTSELARQIADCYGVELIEVLTGFKFIGEKIKEL
                     DEPGIKKFLFGFEESYGYLAGTFARDKDAVVASMLICEMAAYYRNRGMSLYEGLQEMF
                     RKYGYFIEGITSFTLEGKEGIEKIGAAMDALRGMKSYDIDGCEIIAVRDYLTQRRTDM
                     ADGSSRALDLPVSNVLYYELKDMAWFCIRPSGTEPKIKIYYGVAEKTMEEAQLRLDRI
                     KAGVTGMIRKLLGLD"
     gene            complement(3929..>4624)
                     /locus_tag="PLG72_09425"
     CDS             complement(3929..>4624)
                     /locus_tag="PLG72_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOJ81054.1"
                     /translation="MLEAVRNVFDRVQRIREAKDSLKKFSDYEFVGKADLVEINAGSG
                     NILMNIRTPDDLFYSVLSDITGLRIEGEHMLMGMMKELARIRREYERVEYALHEVKMK
                     GYGVVTPQMEEMTLDEPEIIKQGNRFGVKLKASAPSIHMIRADIETEIAPLVGTEKQS
                     EELVNYLLKEFENEPGKIWESNIFGKSLHELVSEGLHNKLMRMPEDAQQKLQETLQRI
                     INEGSGGLICIIL"
BASE COUNT         1023 a         1268 c         1011 g         1322 t
ORIGIN      
        1 cacctcgcag atatcgacga taaaaatacc tgtttcctgt gagaggtcta tttccctcga
       61 agtgcgtttc atgtcctcgt cgtagacgat gcgtaccaac ggttttgtgg gcattttccg
      121 gttgtgtttc acaacgatgg cgcgctccct ggtgttgagc agcacacctg acccttccgg
      181 atatacggtt acgaatctga caaaattctc tacgaccacg gtatcaaaat ggtggagacc
      241 catggatgtg atgtattcat aaacctcatg tggtttcagt ttcttcctgt aaacacggtc
      301 cgaagtgagg gcatcataaa catccgccac cgccactatc ctggcatata tctgtatctc
      361 ttcgcttttc aggcggtacg ggtatccgct cccgtcgtac ctttcatggt ggcccaaagc
      421 gatatacgac gatatgagat tcaactgccc gctttttttc agcagttcat acccaagtat
      481 ggtatgcttc tttatttcct cgaattctgc cacggtaagc tggcttggtt ttttcagtat
      541 ttcccttggt atgcaaagct tgcctatgtc gtggagtatg gcgccaagtc caagttcccg
      601 gagctgcgct gtatcgaaac caagaccgat gccggttatt atcgaaagta tgcacacact
      661 gacagaatgc ttgaaagtgt agtcatccac agtttttatt tcagtaaggt tgtaaaggat
      721 gtcatcgttg gaaagcagtt catccacgat cctgttaacg attgtcttaa catgtcccac
      781 atcgatggcg tcggaaaagc tgtacccggt catgatatcc ctgaccagta ccacggcttc
      841 attgcgtgtt ttttcgttaa cgacgtcgtt gacctcaatg ccttccgaca ggtcgtcatc
      901 caggtatatc tcgtatatac cgcgtttcct aagcagttca agatagctat ctctgagttc
      961 gacgccctgc atgagcaata cgccgccatc ggagctgaaa accgtgcggg cgagccttgc
     1021 accttcaggc actgagtcaa ctttcaccct tctcattcgt tcctccggtt ttattctttg
     1081 gtatggtctt gtttcaaggc acgatagtaa cttattcgac acaacagtat aaaatccttc
     1141 cctgtttcag aggttttaaa attttatttt gggcaataaa caggtttcat ccattttgtg
     1201 gtaatataag tatatatatt tacaaaggag tcgcgccatg atcagatcaa tcccgggaga
     1261 tatcgcaaag cacggttttc ttttgatccg gatagccgtg acagccagga gaatattgag
     1321 caaactcttg tcgttcaggc ccgcagacat gctctcagat gacatgagat accagttgta
     1381 tttctacagg agttatttgc ccgaactgta ccgaaggcat gtcagcaaga actacaaaaa
     1441 ctgtctcttc tgctccaggc ttgtaaaatc gggatgtcac aggtactgtc tcaaatgcag
     1501 cgccggtacc tacataaaat gctatcctcc tctgcccggc cttttatatg cggtcccggt
     1561 gcacacggac gacggggatc agcttgccgt cccatggtat ttcagaaaaa cctgctccgg
     1621 cttcaaaaga ttgcccctgt tttggtattg gcacaatata tattcggtat ttccttcgat
     1681 agccctcagc aactatgaag cgctcgaagg gcttgaaaaa ggcctgtctt ccggtgccag
     1741 gccgtgccac atatgcgcaa ccgtcgatta tgaactttac aggaagtgta tgaagcagcc
     1801 ggattttgac gtttcagccc cgtgcacgaa gataagggaa gaactgaaaa aagcatatcg
     1861 cggccagact gccggcaaac ctgaaaaaag ccgggcaaca agccttaagt ctcaagatcc
     1921 gcagtataaa catacggaaa ctgcggtata aaaaactgca gcaacaggtt tatgacctga
     1981 tgctgcagcc gtcggtccgc tacagccatt gatcagccgg atacattccg gtatatatgc
     2041 ctttgcctgc tgggactgtc cataccggta tcagtcgagg ccgagcagtt tcctgatcat
     2101 ccccgttaca ccggccttta tcctgtcaag cctgagctgt gcttcttcca tcgttttttc
     2161 agctacgccg taatatatct ttatcttcgg ctctgtaccg gaaggcctga tacagaacca
     2221 tgccatgtcc ttcagttcat agtacagcac gttggatacc ggcagatcca gcgctctgct
     2281 gcttccgtca gccatatccg tccttctttg cgtcagataa tccctgaccg cgataatttc
     2341 gcagccatcg atatcgtatg acttcattcc cctcaacgca tccatagcag caccgatctt
     2401 ttcgatgcct tccttccctt ccagcgtgaa ggaggttatg ccctcgatga aatagccgta
     2461 ttttcggaac atttcctgca gcccttcata cagcgacatg cctctgttcc tgtaatatgc
     2521 ggccatctca catatcagca tgctggcaac gactgcgtcc ttgtcccttg caaatgttcc
     2581 ggccaggtat ccgtagcttt cttcgaaacc gaagaggaat ttcttgattc ccggttcgtc
     2641 aagctccttt attttctctc ctatgaactt gaaacctgtc aatacctcta tcagttctac
     2701 gccgtaacag tccgctatct gccttgccaa ctcgctcgtc acgatggtct tgaccacaaa
     2761 accgttttca ggaaggcacc cggcagcctt tttctgcgac aggatgtatt ccatcagcag
     2821 gcatcccgtc tggttgcccg tgagcaccgc atactcgccg gccgaattcc tcacgacgac
     2881 tcctaccctg tcgctgtcag ggtccgtgcc gataattata tccacgtctt ctttcctggc
     2941 aagctctatg gccagcctga acgcatcctt ctcctcggga ttcgggtact tcacggtact
     3001 gaattcagga tccggaagtt cctgttccct gaccaccagc acgtttttga aacctgcttc
     3061 ggcaaggatc ctcctgaccg gcttgttgcc ggaaccatgc aaaggtgtat agacgatctt
     3121 catcgaatcc gccgcttttt tgatctcatc gggattcacc aggagagttt tcaggctttc
     3181 gatatatgcg tcgtccacct ctttgccgat catcctgatc aggcctttgc ccaatgcttc
     3241 cttttcgtct gcgggtttta tggccgtgat atcggttatc ccgtttatct ccttcagaac
     3301 tgcctcggaa ccttcaggcg gcagttgtcc gccgtcctct ccgtaaacct tgtagccgtt
     3361 gtactgtttc ggattgtggc ttgcagtgac gaccacgccg gctgcggcct taagatgcct
     3421 tacggcaaac gacagttcag gagtcgggcg gagttcgtcg aaaaggtatg aaacgatgcc
     3481 gttggctgca aatactcggg cagcttccct tgcaaactcg ggtgacatgc gcctggaatc
     3541 atacgctata actacgccgc gcgccgctgc gtctttcacg ttgttgagaa gatagttggc
     3601 aagcccctgg gaagccttcc ttacggtgta tatgttcatc ctgttcgtgc ctgccccgat
     3661 tatgcccctg aggcctcctg tgccgaattc caggtccttg tagaacctct cctctatttc
     3721 gtccggatca ttgctgattt tctccagttc tttcttcgtt tcctggtcga agtaatcact
     3781 gcgcatccaa aactcaaatg cttccctgct gttcatgttt cccctccaga tgttgctgtt
     3841 gttgtattta ccttgattat tttaccataa aaaaactgaa ataaaagaag aatattggat
     3901 ccaataaaaa aagggctgtt gccccattct aaagtattat acatatcagt ccgccgctgc
     3961 cttcgttgat gatcctctga agcgtctcct gcagcttctg ctgggcatct tccggcatcc
     4021 tcatgagctt gttgtgcagg ccttcactca ccagttcgtg cagcgatttg ccgaatatgt
     4081 tggattccca tattttcccc ggctcattct cgaattcctt aagcagataa ttgacaagtt
     4141 cctcggactg tttctccgtg ccgacaagag gagctatctc tgtctcgata tctgcccgga
     4201 tcatatgtat cgacggtgcg ctggccttga gcttgacgcc gaacctgttg ccctgtttta
     4261 tgatttcggg ctcatccagc gtcatctcct ccatctgcgg cgtcacgacc ccataaccct
     4321 tcattttcac ttcatgcagc gcatactcga cccgttcgta ttctctcctg atccttgcaa
     4381 gttccttcat catccccatc agcatgtgct cgccttcgat cctcagtccc gtaatgtcgc
     4441 tgagtacact gtagaagaga tcatcagggg tccggatgtt catgaggatg ttgcctgaac
     4501 cggcattgat ttcgaccagg tcggcctttc cgacgaattc atagtctgag aactttttca
     4561 gcgagtcctt tgcctcacgg atcctctgta ccctgtcaaa tacgttcctt acagcctcaa
     4621 gcat
//
LOCUS       DAOSCC010000194         4577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_11909_length_4577_cov_4.223795, whole genome shotgun sequence.
ACCESSION   DAOSCC010000194 DAOSCC010000000
VERSION     DAOSCC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11909_length_4577_cov_4.223795"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLG72_09430"
     CDS             complement(<1..776)
                     /locus_tag="PLG72_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ81055.1"
                     /translation="MIDTDHGRNGLIGSFHMLHDNEELLAKVIDFFPYPLQVYAPDGT
                     SVLVNKALLDEYHVDSPERVIGKYNVLTDPAVIATGKYDLLLRAFKGETVYGYDIRVP
                     IEDIKKKYGVHDLDIIAAYQDITVFPILDNQQRVKYVVALMIDRRVYRGKEEIEKARE
                     YIESHWQEPFSISETAKAACLSRAHFSRLFRKHTGMTPYEYYINCKIDKLKEKLLDAN
                     LSVKQAFAQCNMDYNGHSARIFRQKVGLSPSAYRKAVQGKG"
     gene            complement(866..2074)
                     /locus_tag="PLG72_09435"
     CDS             complement(866..2074)
                     /locus_tag="PLG72_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ81056.1"
                     /translation="MKYRKMGKTGDEVSVLGFGCMRFPQKNGRIDLARTERQILMAID
                     RGVNYFDTALIYHGGRSESILGDILAKGYRDKVRVATKLPPFAVHSMKDMDNILNSQL
                     KKLRTDHVDYYLLHAVNDLGGWERLKRLGIEEFARKAKEDGRIRHFGFSYHGSKEDFK
                     AIIDDYPWDMCQIQYNYIDENFQAGREGLEYAASKGIGVVIMEPLRGGTLVKRMPPEI
                     RKIWDTSGYSRTPADWAFRWLWDQPGITTVLSGMNEESQIEENTALADSIEPGMLTEQ
                     ELRLYDKVREEYFRMMKVGCTGCGYCMPCPAGVDIPFCFSYYNARHFFGARRAQWQYI
                     AFAGGLMSGRPSYASLCRQCGKCEKACPQHIPIREKLREAAADMEPAVIRPFVGLVRK
                     YYSFRSRRKT"
     gene            complement(2177..2953)
                     /locus_tag="PLG72_09440"
     CDS             complement(2177..2953)
                     /locus_tag="PLG72_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ81057.1"
                     /translation="MEVIQRKMAFDEGSHLYEKLDADRIDVVGDIHGCFRELAELLKK
                     LGYEVDEENGLIAGSPEGRTLVFLGDFTDRGPEPVKVLKLAMDAVSSGRARSVRGNHE
                     EKILQKLVKGTTAKEEVMATLSAVKAEGPDFVRSVTDFIEGLPYVILCKNDILIVHGG
                     LKEELQDLDTQDGKLRGRIKRMAIYGDITGRQLPDGRPERLDWAADYKGKKTVIYGHT
                     IVEKVQWRNNTVNIDTGCFRTGILTAVRMPEREFFRNRQA"
     gene            complement(3140..4477)
                     /locus_tag="PLG72_09445"
     CDS             complement(3140..4477)
                     /locus_tag="PLG72_09445"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOJ81058.1"
                     /translation="MQYMFPDNFIWGAATAAYQIEGAYREDGKGENIWDRYSHIPGKV
                     YENHTGDVACDHYHRYEEDIRLMKEIGLKSYRFSISWARVFPDGKGTPNQKGLDFYRR
                     LAEKLNENGIRPAATLYHWDLPQKLQDLGGWANRDSVGWFTDYAAYMFEKLGDQVPVW
                     ITHNEPWVVSFIGNWQGRHAPGITDFKTAVQVAHHLMMSHGQAVKAYRETGGKGEIGI
                     TLNMNPVYPASDSDEDKAAAKMYDEYLNKWFADPVLKGAYPADLAAHLQKMDLFPEID
                     SRDMETIHQPVDFLGINNYYSSFIKYDGSLWPIPAFETTTGKDRTQMGWEIYPEGIHD
                     LLVRLNRDYGGIKILVTENGAAFNDIVNRGGKVEDDNRVDYLYRYLGQIHRAINSGVN
                     VKGYYVWSLLDNFEWGYGYSKRFGIIYVDYKTQQRILKKSALWYRDVIRNNGF"
BASE COUNT          920 a         1363 c         1025 g         1269 t
ORIGIN      
        1 cccttgccct gtaccgcctt acggtaggcc gaaggggaca ggccgacttt ctgcctgaaa
       61 atgcgtgcgg agtggccgtt atagtccatg ttgcactggg caaaggcctg tttgacggat
      121 aggttggcat ccagcagctt ttctttcagt ttgtcgattt tgcagttgat ataatactca
      181 tacggcgtca tacccgtatg ctttctgaac agcctggaga aatgcgccct gctgaggcat
      241 gccgcctttg ccgtttcgct tatgctgaat ggttcctgcc aatggctttc tatgtactcc
      301 ctggcctttt cgatctcttc cttgccacgg tagacccttc ggtcgatcat cagtgcaacg
      361 acatacttca ctcgctgttg gttgtctaaa atggggaaaa ccgttatatc ctgatacgcg
      421 gcaattatat ccaggtcgtg gacgccatat ttcttcttta tatcttcaat gggcaccctg
      481 atgtcatatc cgtaaaccgt ttcgcccttg aacgcgcgaa gcagcagatc gtatttaccc
      541 gttgcaataa cggcgggatc tgtcagcacg ttatatttcc cgatgactct ctccgggctg
      601 tcaacgtgat attcatccag caacgcctta ttgaccagta ccgatgtccc gtccggggca
      661 tagacctgta aagggtaagg aaaaaagtca ataactttgg caagaagttc ttcattgtca
      721 tgtaacatat ggaaagatcc aatgagaccg ttccgtccat ggtctgtatc gatcatctgt
      781 tatccccgcc ctacagagat aatataggaa aatccaaatt acattacaac ttaattatac
      841 tataaaaaac ggcgtccgac accaatcaag tcttccgcct gctcctgaac gagtagtact
      901 tcctcaccag gccgacaaat ggccttatta ccgccggctc catgtcggct gctgcttccc
      961 ggagcttctc ccttataggg atatgctgcg ggcatgcctt ctcacacttt ccgcattgcc
     1021 tgcacaatga cgcgtatgaa ggcctcccgc tcatcaggcc gccggcgaat gcgatatact
     1081 gccactgggc cctcctcgcc ccgaaaaagt gcctcgcatt ataatagctg aagcagaaag
     1141 gtatatccac ccctgccggg cacggcatgc agtaaccgca gcccgtgcag ccgaccttca
     1201 tcatcctgaa atactcttcc ctgaccttat catacaagcg cagttcctgc tccgtcagca
     1261 tgccgggctc gatagaatcg gccagcgccg tgttttcttc tatctgcgac tcttcattca
     1321 tgccggacag tacggtggtg atgccgggct ggtcccacag ccagcggaat gcccagtccg
     1381 ccggtgttct gctgtatccg gacgtatccc atatcttccg gatttcggga ggcatcctct
     1441 ttaccagcgt acctccgcga agcggctcca ttatcacgac acctatccct ttggatgcag
     1501 catattccaa tccctctctg cctgcctgga aattctcgtc tatgtaattg tactgtatct
     1561 ggcacatatc ccacgggtaa tcatcgatga tagctttaaa atcctctttg ctcccatggt
     1621 atgaaaaccc gaaatgacgg atcctgccgt cctccttcgc cttgcgggca aactcctcga
     1681 tacccagcct tttgagtctt tcccatccgc cgagatcatt gacagcatgg agcaggtaat
     1741 agtccacatg atccgtccgg agcttcttaa gctggctgtt gagaatattg tccatatcct
     1801 tcatggagtg gaccgcaaaa ggcggaagct tggtcgccac cctgaccttg tccctgtacc
     1861 cttttgccag tatatcgccg agtatggatt cgctccttcc tccatggtat ataagagcgg
     1921 tgtcgaaata gttcacgccg cggtcgatgg ccatgagtat ctgtctttca gtccgggcca
     1981 ggtcgatcct gccgtttttc tggggaaagc gcatgcaccc gaaaccaagg accgatacct
     2041 catcccccgt ttttcccatc ttcctgtatt tcattcggat ccccctcttc cgtctccctg
     2101 ctgtgccaat tatatattcc tgttccagga accatatttc cgtttgcaga tccacatctg
     2161 cagcttaccg tcaaaactat gcctgccggt tccggaagaa ttccctctca ggcatcctga
     2221 cggcggtcag tatgccggtc ctgaaacatc ccgtatctat gttgacggtg ttgttcctcc
     2281 actggacctt ttcaactatg gtatggccgt atataacggt ctttttgccc ttatagtccg
     2341 cagcccagtc gagtctttcg ggccttccgt ccggaagctg ccggcctgtt atatctccgt
     2401 atatcgccat cctctttatc ctgccccgga gcttcccgtc ctgcgtatcc aggtcctgca
     2461 gttcttcctt caggcccccg tgcactataa gtatatcgtt tttacacagt ataacataag
     2521 gcagcccttc gatgaaatcg gtgacactcc gtacgaaatc cggaccttcc gctttgacgg
     2581 cagacagggt cgccattact tcttcttttg ccgtcgtccc ttttacaagc ttttgcagta
     2641 tcttctcctc gtggtttccc cttacggacc gggcccgccc gctgcttacc gcatccattg
     2701 ccagtttcag aaccttgaca ggctcggggc ccctgtccgt aaaatcgccc aggaatacga
     2761 gagtgcggcc ttccggcgat ccggctatca gcccgttttc ctcatcgact tcatacccca
     2821 gctttttcag cagttcggcc agttccctga aacacccgtg tatgtctcct actacatcga
     2881 ttctgtcagc atccagtttt tcgtacaggt gtgagccttc atcgaatgcc atcttccttt
     2941 ggataacctc catctgttat tgctcttcat gaagtccgac tgccgcgtca cattgcactc
     3001 ctcttttcat cccgccgcga tccgacgccg gtctttggca tctatccgca tatacctgcc
     3061 gcggttcgtc catggaccgg aatgtttgcg cagcagcaca ttactgtcat tatcgggctg
     3121 cgatgtccgg atcagccgat cagaatccat tgttccttat aacgtccctg taccagagcg
     3181 cactcttttt cagtatccgc tgctgcgttt tataatcgac gtatatgatg ccgaacctct
     3241 tgctgtaacc gtatccccat tcgaaattgt ccagcagcga ccacacataa tatcctttga
     3301 cattgacgcc cgaatttatg gcacgatgta tctgtccaag gtacctgtaa aggtaatcca
     3361 cgcggttgtc gtcctcgacc tttcccccgc ggttgacgat atcgttgaaa gccgcgccgt
     3421 tttcggtgac gaggatcttt atcccgccat aatccctgtt gagacggaca agcaggtcat
     3481 ggatgccttc ggggtatatc tcccagccca tctgcgtcct gtctttgccg gtcgtcgtct
     3541 cgaacgcggg tatcggccac agcgaaccgt catattttat gaagctgctg taataattat
     3601 ttattccaag gaaatcgaca ggctgatgta tcgtctccat atcacggctg tctatctccg
     3661 ggaacaggtc cattttctga agatgcgccg ccaggtcggc cgggtatgcg cctttgagta
     3721 cgggatcggc aaaccatttg ttgagatatt cgtcgtacat ctttgccgca gccttgtctt
     3781 catcgctgtc cgaagcggga taaacagggt tcatgttcag cgttatcccg atttctcctt
     3841 tcccgcccgt ctccctgtat gcttttactg cctggccgtg ggacatcatc aggtgatggg
     3901 ccacctgcac ggcggtcttg aaatcggtga tgcccggggc atgcctgccc tgccagttcc
     3961 ctataaatga cactacccac ggctcgttgt gcgtgatcca gaccggcacc tggtcgccga
     4021 gtttttcaaa catgtatgcg gcataatcgg tgaaccatcc gacggaatcc ctgttcgccc
     4081 atccgccaag atcctggagt ttctggggca gatcccagtg gtaaagcgta gcggcgggcc
     4141 tgatgccgtt ctcgttcagc ttttccgcga gcctcctgta aaaatccagt cctttctggt
     4201 tcggggtccc cttcccgtca gggaacactc tcgcccacga tatggaaaag cggtatgatt
     4261 tcaggccgat ctccttcatc agcctgatgt cttcctcata cctgtggtaa tggtcacatg
     4321 cgacatctcc cgtgtgattt tcatatacct tgccggggat gtggctgtag cggtcccata
     4381 tattctcgcc cttgccgtcc tccctgtacg ccccttctat ctggtacgcg gctgtcgcgg
     4441 cgccccatat gaaattgtcc ggaaacatgt actgcatctt tttctcctcc tgttatccat
     4501 attttttgat gttgctcctg ctatcaaccg aaaaatcagc gcccgtatgc attcattata
     4561 ccccatgccg tcatccg
//
LOCUS       DAOSCC010000195         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12048_length_4536_cov_4.185896, whole genome shotgun sequence.
ACCESSION   DAOSCC010000195 DAOSCC010000000
VERSION     DAOSCC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12048_length_4536_cov_4.185896"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLG72_09450"
     CDS             <1..901
                     /locus_tag="PLG72_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81059.1"
                     /translation="RSSLRPTHSCCRKALRTVPMPVVPGAVFTVIIYMVIIMLLKAAF
                     KFRNILILAAGIALIAGAAGYAPDAGWVRIAAGTASLAAYAGAVAATMRSREFKKELE
                     LSEKLEGIYRLNREIDEQYRHISGRLGKNLRQKAARVLRHKDQLLHYFNEYHDDPVKQ
                     KIVQQALKLVMAYLKLAGSLADRAGELSPKSMNELTARINANNRRLGTLKSYQAVLEL
                     SKTIEMDEKLLEMMKEERRDLETASVRLDQIESSIVGFRHRILSNEISDTEYEEIENA
                     INEATALDNALNEQRRRRRESCQ"
     gene            complement(1000..1869)
                     /gene="metF"
                     /locus_tag="PLG72_09455"
     CDS             complement(1000..1869)
                     /gene="metF"
                     /locus_tag="PLG72_09455"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOJ81060.1"
                     /translation="MKIIDIFNRKRPVLSFEIFPPRPDVPLETIFDHLEQFEALMPDF
                     ISVTYGAGGSRKGRTLEIAARIKGLHGIESMAHFTCAGHTREEIDSMLESMHQSGLEN
                     ILALRGDPPADQPGFDFSRGAYRHASELVRHIRKMNDFCIAAAAYVEGHVDSRRISED
                     LSHLKEKVDAGVDFLITQLFFDNRLFFDFIERARAIGIKCPVSAGIMPIFKADQIKSI
                     ALKSGCSIPAKVVLMMDKYQNDPEDMRKAGIEYASLQIRELIEEGVDGIHLYTMNRPK
                     STREIMSNISALL"
     gene            2236..3081
                     /locus_tag="PLG72_09460"
     CDS             2236..3081
                     /locus_tag="PLG72_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ81061.1"
                     /translation="MYKIVRKEVLNPAVKLLEVEAPYVARKAEPGQFIILRISEEGER
                     IPLTIADYDREKGTVTIIFQEVGKTTSMLGMLEEGDCILDFAGPLGKASHLEGVRKAV
                     VIGGGLGTAIAYPQAKKLHELGAEVDVITGFRNSGLILLSKELKQVSSRLFITTDDGS
                     NGNKGFVTDVLKKLLEEGNRYDLAIAIGPLIMMKMVSLLTKEYGIKTVVSMNPVMIDG
                     TGMCGGCRVTVGGKVKFACVDGPDFDGHEVDFDEAIRRQAMYRAQEKRSMEEHVCRLG
                     GVRNG"
     gene            3074..4468
                     /gene="gltA"
                     /locus_tag="PLG72_09465"
     CDS             3074..4468
                     /gene="gltA"
                     /locus_tag="PLG72_09465"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOJ81062.1"
                     /translation="MADMSLEKVKMPEQDPETRIRNFNEVALGYTEEMAVKESHRCLN
                     CKTRPCVAGCPVNVQIPEFIRLVSEGDFERAYEKIKETNNLPAICGRVCPQESQCEKF
                     CVRGKKGEPVGIGRLERFVADWYMANGDMDIQPVPKNGKKVAVIGSGPAGLTCAADLA
                     KKGYEVTIFEAFHVPGGVLMYGIPEFRLPKVLVQKEIDTVRKLGVNIMTNMVIGKVLS
                     LEDLRNEGYEAIFIGSGAGLPNFMGIPGENLNGVYSANEFLTRVNLMKAYKYPEYDTP
                     VSVAESVAVVGGGNVAMDAARCAKRLGAKNVYIVYRRSEAEMPARLEEVHHAKEEGII
                     FKLLTNPVRIVGTDDGWVRAMECVEMELGEPDASGRRKPVPKPGSEHLLDVGTVIIAI
                     GQSPNPLIKSTTPGLETQPWGGIITEEETGATSLDGVYAGGDAVTGAATVILAMGAGK
                     KAAAAIDGYLGRKK"
BASE COUNT         1187 a         1084 c         1292 g          973 t
ORIGIN      
        1 tcgctcctca ctccgtccga cgcattcctg ctgccgcaag gcgctccgga ctgtccccat
       61 gcctgtcgtg cctggcgccg tttttactgt tataatatat atggtgatta ttatgttgct
      121 gaaagcggcg ttcaaattca ggaacatact gatactggct gcaggcattg cgctgattgc
      181 cggcgctgcc gggtatgcgc cagatgccgg atgggtccgg atcgcggccg gaacggcaag
      241 ccttgccgca tatgccggtg cggttgccgc aacgatgaga agcagggagt tcaaaaagga
      301 actggaactg tctgagaaac tggagggcat atacaggctg aacagagaga tcgacgaaca
      361 gtacaggcat atatccgggc ggcttgggaa gaatctcagg cagaaagccg ccagggtcct
      421 caggcataaa gaccagttgc tgcattattt caatgaatat cacgatgatc ctgtaaaaca
      481 gaagatagtg cagcaggccc tgaagcttgt catggcgtac ctgaagctgg caggcagcct
      541 tgccgacagg gcaggggagc tttcgccgaa gagcatgaat gagcttacgg cacggataaa
      601 tgcaaacaac aggaggcttg gcacattgaa gagctaccag gccgtgctgg aactgtccaa
      661 aaccatagaa atggacgaga aattgctcga aatgatgaaa gaggagagaa gggaccttga
      721 gacggcaagt gtccggctcg accagataga gagcagcata gtcggattca ggcaccggat
      781 actctccaat gaaatatccg ataccgaata cgaggagata gaaaatgcga taaacgaggc
      841 aacagccctt gacaatgccc tcaatgaaca gcggcgccgc aggcgggaat cctgtcaatg
      901 actgcggctg cctgatcttg tccctggcaa taatcatcgc tgccgctttt cggtgtatgt
      961 aaagaaatgc ctcccggtca aagtggtacc cgccgaccct cacagcagcg ctgagatatt
     1021 gctcattatt tcccgggtcg attttggacg gttcatcgta taaagatgga tgccgtccac
     1081 cccttcttcg atcagttccc tgatctggag cgatgcgtat tctataccgg ccttcctcat
     1141 atcttcaggg tcgttttggt atttatccat catcagcacc acctttgcag gtatcgaaca
     1201 cccgcttttc aacgctatcg attttatctg gtcagctttg aaaataggca ttatgcctgc
     1261 tgaaacgggg cactttattc cgatcgccct tgccctttcg atgaagtcga aaaacagcct
     1321 gttgtcgaag aacaactgcg taataaggaa atcgacgcct gcatccactt tctccttcag
     1381 gtgtgacagg tcctcgctga tcctcctgga atccacatgg ccttcaacat atgcagccgc
     1441 ggcaatgcag aaatcattca ttttgcggat gtgccgcacc agttcgctgg cgtgtctgta
     1501 tgcccccctg ctgaagtcga aaccgggctg gtctgccggc gggtcgcccc tcagcgcgag
     1561 tatgttctca aggcctgact ggtgcatcga ttccagcatg ctgtctattt cttccctggt
     1621 atgacctgcg catgtgaaat gggccatgct ctcgatgccg tggagacctt ttatcctggc
     1681 cgctatttca agcgtacggc ccttcctgct cccgccggcc ccgtaagtga cgctgatgaa
     1741 gtccggcatt agggcttcga actgctcaag atggtcgaat atcgtttcca gcggaacatc
     1801 cggcctgggc gggaatattt caaaggacag taccggcctt tttctattga aaatatcaat
     1861 tattttcaca catccaacct cccgaaataa agctaactac attataacaa catttaaata
     1921 gctgtcaagt acaattaaaa agcagttata ttcattcaag cagccccggc atgcacaggt
     1981 ttttgcttcc gggaatcaat atctgccaaa gggatttccg gcagtgggac acttctgcgg
     2041 acaagcgctc aataagcttt cttcaggaca ggacacacat gtaaatatct ccaattactc
     2101 ccgctttata gcgaatggat gttatcgtta ttgcggattt tgtgacagta gtttaaaata
     2161 tcaggtgaca tgtataagca tgaatcgtat gttatgtcgg cagccagctg tccggcatca
     2221 gtaatggggg tttttatgta taaaatcgta aggaaggaag tactcaaccc ggcagtgaag
     2281 ctgttggagg tagaagcgcc gtatgtggcc agaaaagcgg aacctggcca gttcataata
     2341 ctgaggataa gcgaagaagg cgaaaggatc ccgttgacga ttgccgacta cgaccgggag
     2401 aaaggaacgg ttaccatcat attccaggaa gtggggaaga ccacctcgat gctggggatg
     2461 cttgaggaag gcgattgtat actggacttt gcaggccctc ttggcaaagc gagccacctg
     2521 gaaggagtga ggaaagccgt ggtcatcggc ggaggccttg gtacggccat agcatatccg
     2581 caggcaaaga aactgcacga actgggtgca gaggtggatg tcatcacggg ctttcggaac
     2641 agcggcctga tcctcctttc gaaagaactc aaacaggtaa gcagcaggct gttcatcacc
     2701 actgacgacg gttccaacgg caacaagggt tttgttacgg atgtactgaa aaaactgctt
     2761 gaggaaggca acaggtacga tctggccatt gccatcggtc cgcttataat gatgaagatg
     2821 gtatcgctgc tgacgaaaga gtatggcatt aagaccgtcg tcagcatgaa tcctgtcatg
     2881 atagacggca ccgggatgtg cggcggctgc cgtgtcacgg ttggaggcaa agtaaaattc
     2941 gcctgtgtcg acggacctga ttttgacggc cacgaggtgg atttcgacga agcgataagg
     3001 agacaggcca tgtacagggc acaggaaaag aggtccatgg aagagcacgt ctgcaggttg
     3061 ggaggtgtcc ggaatggctg atatgtcttt ggaaaaggta aaaatgccgg aacaggaccc
     3121 ggaaaccagg atcaggaatt tcaacgaggt agctctcgga tatactgagg aaatggcggt
     3181 aaaggagtcg cacaggtgcc tgaactgcaa aaccaggccg tgtgtcgccg gctgtcctgt
     3241 aaacgtacag ataccagaat ttatcaggct tgtaagcgaa ggagatttcg aaagggctta
     3301 tgaaaagata aaggagacca acaaccttcc agccatatgc ggaagggtat gtccgcagga
     3361 gtcccagtgc gagaagttct gcgttagggg aaagaagggc gaacctgtcg gtatcggaag
     3421 gctggaaaga ttcgttgccg actggtacat ggcaaacggg gacatggata tccagcctgt
     3481 cccgaagaat ggaaagaagg tggccgttat aggatccggg ccggcaggac tgacatgcgc
     3541 ggccgatctg gccaaaaaag gatatgaagt cacgatattc gaagctttcc atgtgcccgg
     3601 cggcgtgctg atgtacggga tcccggaatt caggctgccg aaggtcctgg tgcagaaaga
     3661 aatagatacg gtccgcaagc ttggggtgaa catcatgacc aatatggtca taggcaaggt
     3721 actgtcgctc gaggatctca ggaatgaagg ctacgaagcc atattcatag gcagcggcgc
     3781 aggcttgccc aatttcatgg gcatccccgg cgaaaacctg aacggcgtat actcggctaa
     3841 tgagttcctg accagggtaa accttatgaa agcatacaag taccctgaat acgatactcc
     3901 tgtgagcgtg gccgaaagcg ttgccgtcgt aggcggcggg aatgtggcaa tggatgccgc
     3961 aaggtgcgca aaaaggctcg gggcgaaaaa cgtatacatc gtatacaggc gctccgaggc
     4021 tgagatgcct gcaaggcttg aggaagtgca ccatgcgaag gaagaaggca taatattcaa
     4081 gctgctgacc aacccggtcc gcatcgtagg cactgacgac ggctgggtca gggcgatgga
     4141 atgcgtggaa atggagcttg gcgagccgga cgcctcgggc aggagaaagc ctgtaccgaa
     4201 gccgggttct gagcatctgc tggatgtcgg cactgtcatc atagctatcg ggcagtcgcc
     4261 gaacccgctc atcaagtcga cgactccagg acttgaaaca cagccgtggg gaggcatcat
     4321 cacggaggag gagaccggcg ccaccagcct tgatggagtc tacgcaggcg gcgacgcggt
     4381 caccggcgct gctacggtca tactggccat gggcgcaggt aaaaaagcag cagcggcgat
     4441 cgacggatac ttgggcagga aaaagtgaaa tatggagcgt gaaacagccg ccggaagatg
     4501 aaaaatcttc cggcgacttt gtttcgttag cgtaaa
//
LOCUS       DAOSCC010000196         4483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12221_length_4483_cov_4.104788, whole genome shotgun sequence.
ACCESSION   DAOSCC010000196 DAOSCC010000000
VERSION     DAOSCC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12221_length_4483_cov_4.104788"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PLG72_09470"
     CDS             <1..1467
                     /locus_tag="PLG72_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ81063.1"
                     /translation="EKNGIRVDVPKLSEMLGVSVIPTKAVKNVGIAEVLDRIGDASQG
                     NRLAGVAELLKPYTEKGLTEPEALMVLEDDGETCKRVGMQPAGLQEKLYRQRRERVDE
                     IIAAAVTETDDKASFSVKLGRLMLRPLTGIPMLIATLILVFMFVGVFMGQTVVELTEE
                     KIMEGIYRPFIADLTGKVIPADSFLGQILAGEFGLLTMTPVYILGLLLPLVVGFYLML
                     SILEDSGYLPRIAVLTDKVMTRVGLNGRAIIPTILAFGCFTAATMTTRLLGSRRERFI
                     ATALLGLTIPCSAQLGVIAGMLSSLGLPYILFYVFVLLLLFGLVGRLLNRLLPGESTG
                     LLIDLPPIRLPGVKNVLKKTFVKTKMFLKEATPLFALGALIISLLQYFGILEAIQAAI
                     APLTEQWLMLPKEMATVFIMGMIRRDFGAAGLSSLALTGPQLMTALITITLFVPCIAS
                     VIVIFKERSKGEAAVIWLGSLVSAFLVGGLVARFLLLV"
     gene            1709..2146
                     /locus_tag="PLG72_09475"
     CDS             1709..2146
                     /locus_tag="PLG72_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOJ81064.1"
                     /translation="MDGSQKNDLMELLKKSGYKFTDQRSAVLDVLIRHKDSHLSIDEI
                     YNYVKENNPDIGIATVYRTLTLLEKVGAVVKLDLDDGFSRYELSRQDEDHRHHHLICT
                     KCGDVSEVEDDLLDALEEQILQKDGFLVTDHRVKFYGLCKKCR"
     gene            2258..3070
                     /locus_tag="PLG72_09480"
     CDS             2258..3070
                     /locus_tag="PLG72_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ81065.1"
                     /translation="MDALFSNLSPVVQALIATGFSWFMTAVGSALVFFFKSINKRVLD
                     TMLGSAAGVMIAASFWSLLAPSIEMSEELGLPVWMPPLVGFLAGGFFLKLADSLLPHL
                     HIGSPSEKAEGIKSGWKRVVLLVFAITLHNIPEGLAVGVAIGAATNNLGSATLASALI
                     LTLGMGLQNLPEGAAVSIPLRREGVSRLKSFWYGQVSGLVEPIAGVLGVVAVMAIKPI
                     LPYAMSFAAGAMIYVVVEELLPEAQSSDSSDYSTIGCILGFALMMVLDIALG"
     gene            complement(3217..4050)
                     /locus_tag="PLG72_09485"
     CDS             complement(3217..4050)
                     /locus_tag="PLG72_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ81066.1"
                     /translation="MVRKIVKINEEKCNGCGLCINACHEGALVLENGKAKLVSDAYCD
                     GLGNCLPECPTGAIEIIEREAEAFDEAAVNKRLGEMGLPPVQNGQDPDQGGHGAGNQE
                     SPASHSHAFHSASGCPGSRMRFISRNNDPDTTGNNSTPHTATGSPCSGADTKPESQLR
                     QWPCQIKLVSPNAPYFHNAHLLVAADCTAYAYASIHQEFMKGRITIIGCPKLDDTDYS
                     EKLAAILANNNIQSITVLRMEVPCCGGLVQAVRDAMLTSGRIIPWNVVTISIDGNIIN
                     Q"
BASE COUNT         1006 a         1065 c         1241 g         1171 t
ORIGIN      
        1 gagaagaacg ggataagggt cgatgtgccg aagctttcgg aaatgcttgg agtttccgtg
       61 atccctacga aggcagtgaa aaatgtcggt atagccgaag tactggaccg gatcggggac
      121 gcgtcgcagg gcaacaggct ggcaggcgtt gctgaactcc ttaagccata tacggagaaa
      181 ggcctgacag agcctgaggc actcatggtg ctcgaagatg acggcgagac ctgcaaaaga
      241 gtcgggatgc agcctgccgg gctgcaggag aaactgtaca ggcagaggcg tgaaagggtc
      301 gatgagataa tagcggcagc tgtgaccgag accgacgaca aagcaagttt ttccgtaaag
      361 cttgggcgcc tgatgctcag gccgctgaca gggatcccga tgcttatcgc aacattgatc
      421 ctggtattta tgttcgtggg cgtcttcatg gggcagacag tcgttgaact gactgaagag
      481 aaaataatgg aagggatata ccggccattt atcgctgacc tcaccggcaa ggtgatcccg
      541 gcggactcgt tcctgggaca gatccttgca ggagagttcg ggctgctcac gatgacgccg
      601 gtctatatac tgggtcttct gcttccgttg gtcgtcgggt tctacctgat gctgtccata
      661 ttggaggatt caggttacct gccgaggata gccgttctga cagacaaggt gatgaccagg
      721 gtcggactca acggaagggc gatcatcccg accatactcg ccttcggatg cttcaccgct
      781 gccacgatga caacgagact gttgggctcc aggcgtgaac gcttcattgc caccgctctt
      841 ttggggctga ccataccgtg ctccgcacaa ctgggcgtca ttgcgggcat gctctcatcg
      901 cttggcctgc catatatact gttctacgtg ttcgtactcc tattgctgtt cgggctggtg
      961 ggcaggctcc tgaacaggct tttgccaggc gaatccaccg gcctcctgat agatctgccg
     1021 ccaatacgcc tgcctggcgt caaaaatgtg ttgaagaaaa cgtttgtaaa aactaaaatg
     1081 tttttaaagg aagccactcc gctttttgcg ctgggagccc tcatcatttc actgcttcag
     1141 tatttcggga tactcgaagc gatacaggct gctattgcgc cgctgacaga gcagtggctg
     1201 atgctgccga aggaaatggc gaccgtgttc ataatgggta tgatcagacg tgatttcggt
     1261 gcggcagggc tcagcagcct ggcactgaca ggcccgcagc tgatgacggc gctgatcacg
     1321 ataacgctgt tcgtgccgtg tatcgcttca gtgatcgtta ttttcaagga aagatcgaag
     1381 ggcgaggccg cagtcatatg gctgggaagc cttgtatcag cattcctggt tgggggactg
     1441 gtcgcacggt tcctgttgct ggtttgacga tagtggtata atggaagtga tatgtatgaa
     1501 gacagtatgc gctgtatgcg gttatgaata tgaaagtgac aggccggaaa aatgtccgcg
     1561 atgcggttcg gccccacgga tcgcgggcgg ctgtgaaaat tgccggggat gttcgctttg
     1621 gggatcgtgc agtatcagga acacaaacgg tgcacagcac gataaggaaa gaaaggcggt
     1681 aaaatgaccc cgacaggagg gaaaaccgat ggatggatcc cagaagaatg acctcatgga
     1741 actgctgaaa aagagcggtt acaagttcac cgaccagaga agtgctgtgc tggatgtgct
     1801 gatcaggcat aaagacagtc atctcagtat cgatgaaata tataattatg ttaaggaaaa
     1861 caatccggac atcgggatag ctacggtgta ccggactctg acgcttcttg agaaagtcgg
     1921 ggcagttgtg aaactcgacc tggacgacgg gttcagccgg tatgagctgt cccgccagga
     1981 tgaggaccat cgccaccatc accttatctg caccaaatgc ggcgatgttt cagaggtcga
     2041 agacgatctg ctggacgcat tggaggagca gatacttcag aaagacggtt ttttggtcac
     2101 ggatcacagg gtgaaattct acggcttgtg taaaaagtgc aggtaaatga ccgtactggt
     2161 tccgatacat ttatctgttt gaattatgtt tggggtatct ttgccggaca gagcgctgtg
     2221 atcggcataa agagtattgc attatggagg tcttcgtatg gatgcgttgt tcagtaattt
     2281 aagtccggtc gtgcaggcat tgatagccac cgggttttca tggttcatga cggcagttgg
     2341 atccgccctt gtattctttt tcaagtctat aaacaaaaga gtcctcgata cgatgcttgg
     2401 gtcggcggcg ggtgttatga tcgcggcaag tttctggtcg ctgctcgctc cctccataga
     2461 gatgtcggaa gagcttggcc tgcctgtatg gatgcccccg ctcgtcgggt tccttgcagg
     2521 cggatttttc ctgaagctcg cagattctct gcttccgcac ctgcacattg gttcgcccag
     2581 tgaaaaggca gaaggcatca aatcgggatg gaagcgcgtc gttttgcttg tgtttgcgat
     2641 cactttgcat aatatacctg aaggccttgc cgttggcgtc gcgataggcg cagcaaccaa
     2701 caacctggga tcggctacac tggcaagcgc tctcatactt actctcggta tgggcctcca
     2761 gaacctgcct gaaggagcgg cagtttcgat accgctcagg cgggaaggag tttcaaggct
     2821 caagagtttc tggtacggcc aggtttcagg gctcgttgag ccaatagccg gcgtgctggg
     2881 ggttgtcgcg gtcatggcga taaagccgat actgccgtat gcaatgtcct tcgcggcagg
     2941 cgccatgata tacgtggttg tcgaggaact gctgcctgaa gcccagtcgt cggacagttc
     3001 cgattattcc accataggat gtatccttgg ctttgcgctg atgatggtgc ttgacatagc
     3061 attgggatga cagaaaagca gtcaagcagc acgttgctga ttacgtgctg ctttatgcga
     3121 tgctgctatc tgagatgctg tttctacacg gtgccctgcc atacatgctg cctgttatgc
     3181 atgcttccat tgaatatttt atctgttgtc tcccgactat tggtttatta tgttgccgtc
     3241 gatacttatt gtaacaacat tccagggtat tatcctgccg ctcgtcagca tggcgtccct
     3301 tactgcctgg accagtccgc cgcagcaggg aacttccatc ctgaggaccg ttatgctctg
     3361 gatattgttg tttgcaagta ttgcggcaag cttttcggaa taatccgtat cgtcgagctt
     3421 cgggcatcct attatcgtga tcctgccctt catgaattcc tggtggatag acgcgtatgc
     3481 atacgcagtg cagtccgcag ctaccagcag atgcgcattg tggaaatacg gcgcattggg
     3541 tgaaaccagt ttgatctggc agggccactg cctgagctgt gattccggtt tcgtatctgc
     3601 gccggagcac gggctgcctg ttgctgtatg aggtgtgctg ttattcccgg tcgtatccgg
     3661 atcgttattc ctgcttatga agcgcatgcg cgaaccggga cagccggacg ctgaatggaa
     3721 cgcatggctg tgtgaagccg gactttcctg attgcctgct ccgtgtccgc cctggtccgg
     3781 atcctgacca ttctgtacgg gaggcagacc catctccccg aggcgcttgt ttacggctgc
     3841 ttcatcgaat gcttccgctt cacgctcgat tatctcaatc gcgcctgtcg gacactctgg
     3901 aaggcagttc ccgaggccgt cgcagtaagc gtcagatacc agttttgctt taccgttctc
     3961 cagcacaagg gccccttcgt ggcatgcatt tatgcagagc ccgcatccat tgcatttctc
     4021 ttcatttatc ttaactattt tcctgaccat gttattgctc ctttcctgca ttttccgtgc
     4081 cgacacgtca cattcacggt cattgtccgt atatatgtgc ctgcatgtcc tgcctggcgc
     4141 catgcttttt tctttgtacc tctattgtag taccggacca tatgtgaatc ggtaaccaag
     4201 gttacaaaaa tccattgatg ttttgctgcc gcagaatttt aatgtggttt ggatatatat
     4261 tccaaacaac tgtgccataa ttaggcgacg gattatatat ttgtcatatt tgtcctgttg
     4321 aaacatttct tttgaataat gtgctataac gagtcgatgt agggctcata tgcctcgctg
     4381 aaacacttct gttgtgaaga aattttttta caaacagaaa attttctttc tataaatgag
     4441 caaaagcgct aacatataat gtaaaattaa aggagacacc ttg
//
LOCUS       DAOSCC010000197         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12446_length_4422_cov_4.979849, whole genome shotgun sequence.
ACCESSION   DAOSCC010000197 DAOSCC010000000
VERSION     DAOSCC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12446_length_4422_cov_4.979849"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLG72_09490"
     CDS             <1..683
                     /locus_tag="PLG72_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ81067.1"
                     /translation="ACKNNGVKLQIGFMRRFDASFREARRLLEEGVIGDLVQIHSCTR
                     GPSKPRPWMYDLKKSNGILAEVNSHDIDSIRWFAGSEIKELYAVAGNFRNPEAKAEYP
                     DYYDSVLMNGIFENGVQFTVDGAAYVQYGYDSRMEIVGTKGVLHVGRSDASFVKCTTV
                     EHGTSTPFISSWTTLFADAYLAEDIAFAEAIINDTEPAVTGWDGRQAVGIVEAGNRSI
                     LTKSIVRL"
     gene            730..1767
                     /locus_tag="PLG72_09495"
     CDS             730..1767
                     /locus_tag="PLG72_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ81068.1"
                     /translation="MLSLKLYGPNDIRLTETDIPETGDGELLLKTDAAAVCGTDIRMW
                     QNGYKGVDEEHPLTLGHEFSGTIVRVGKNVPFYKEGMKVAVQPNIGCGICDRCVSGNF
                     HLCDDYRAFGINMDGAFAEYVKIPADAVLRGNLMLLPDGVTAEEAAVTEPLSCAYNGF
                     TKCFVKPGEYALVVGAGPIGVSHAMLLHMAGASVLMNDLSQERLDLCKKILPYIETYS
                     GNDLAGFVKDWTRGRGLDIAITACPVPQVQSAMLPLMNYGGRVNFFGGIPANKQPVPI
                     DTNLVHYRELYLTGSTRSSIAQFRKTLEFVSQGLINVNAMITDRYELKDIRDAFENVR
                     AAKGIKHVITF"
     gene            1855..3183
                     /locus_tag="PLG72_09500"
     CDS             1855..3183
                     /locus_tag="PLG72_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81069.1"
                     /translation="MKVRLVPLHFAEANERERQEFKDQMARLRDYYGDVAEFLPEVCV
                     GDPLPEADAIVFPQLIFAAFRHNDEMKAYDLPMIVLTSRFGTVEMWDWEIVTYLRDLG
                     CTVFSPYSIEMAKTILRAIAVRHSLKQGARFLMFQDSPGEGMQANIFKRFYWWEKEST
                     ETMEEVFGCKLIYRSWKEVGDRAKQISDEEALKISEDWEINQEGVSRMAFLRAVKIYI
                     AVKEVISEIGDVAGVGSNCLNESFNCDTTPCLAWNMLFERDNIIWACEGDTLTLLSTY
                     IIYRSIEKPIMMTNLYPFLVGMAALAHEKIDRFPDIEDPDNHALGVHCGYFGFAPRNF
                     CTQWTLRPKVLEIVNDDAIVIDCCFPMGKVTLAKIYPGFKKLSVIEAEIEDYVQYPGS
                     DCRNGALIRYKDGHRVMDELCSHHSLIIAGDVRPQLRQLAKVFGFSVTEL"
     gene            3667..>4422
                     /locus_tag="PLG72_09505"
     CDS             3667..>4422
                     /locus_tag="PLG72_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ81070.1"
                     /translation="MMIGASSYIWVSPFSTEELEQLSLAKGLGFDVYEIAVERPELID
                     DEKVLAEANRAGIKVHVCGAYGETRDISSDVPGYRENGMAYGKRLIDMAAVLGSPYVA
                     GPMFAAVGRTRLADTGEKQRQMDYAVENMKRLAGYAANRGIKLAIEPLNRFETDFINT
                     VEQGLYFIDRIGYDNVGFLLDTFHMNIEEKSIPCAIRSAKGRIFDFHACANDRGTPGE
                     DHFDWEEIRKALDDADYKGPVVIESFTPNITEIA"
BASE COUNT         1155 a          985 c         1259 g         1023 t
ORIGIN      
        1 aagcgtgcaa aaacaacggc gtaaagctgc agataggctt tatgcgccgt tttgacgcaa
       61 gcttcaggga agccaggagg ctgctagagg aaggcgttat aggagacctg gtccagatcc
      121 attcatgcac gcgcggcccg agcaagcccc gtccgtggat gtatgacctg aagaagtcaa
      181 acggcatact tgccgaggtc aacagccacg atatcgattc gatacgctgg ttcgcaggca
      241 gcgagataaa ggaattgtat gcggtggcag gcaacttccg gaatccggaa gccaaagccg
      301 aatatcctga ttattacgac agcgtcctga tgaacggcat ttttgaaaac ggcgtgcagt
      361 tcaccgtcga cggcgcggcc tatgtccagt acggatatga ttcaaggatg gagatcgtgg
      421 gaactaaagg agtgctgcat gtgggcaggt cggatgccag ttttgtcaaa tgcaccacag
      481 tggagcacgg gacttcgacg ccgtttataa gcagttggac gacgcttttt gcagatgcat
      541 atctggctga ggacatcgcg ttcgcggaag cgataataaa cgataccgaa ccggcagtga
      601 ccggctggga cggaaggcag gcagtgggca tagtcgaggc cggcaaccgt tcgatattga
      661 cgaaaagtat agtgaggctg taacggccgg atcatatatc agtgtggatt gtgtaaagga
      721 gggtcatgaa tgctcagttt gaaactgtac ggcccgaatg atataaggct gacagaaacc
      781 gacataccgg agaccggcga cggcgaactg ctgctaaaaa cggacgcggc ggctgtttgc
      841 gggaccgata tacggatgtg gcaaaacggt tacaaaggtg tggatgaaga gcatccgctc
      901 acattaggtc atgaattttc cgggaccatc gtcagggtcg gcaaaaatgt gcctttttac
      961 aaagagggca tgaaagttgc agtacagccg aatatcggct gcggcatatg cgaccgatgt
     1021 gtatcgggca atttccatct ttgcgacgat tatcgcgcat ttggcatcaa catggacggt
     1081 gcgtttgcag agtatgtgaa aatacccgca gatgctgtac ttcgaggcaa cctcatgctg
     1141 ctgcccgacg gcgtgaccgc cgaggaggcg gcagttacgg aacccctttc atgtgcgtac
     1201 aacggcttta caaaatgctt tgtcaagccg ggcgaatatg cgctggtggt aggcgccgga
     1261 ccgataggcg tcagtcatgc gatgctgctc catatggcag gcgcgtcggt actgatgaac
     1321 gacctgtcgc aggagaggct ggatctctgc aaaaagatcc tgccgtatat cgagacttac
     1381 agcgggaatg acctggccgg cttcgtgaag gattggacgc gcgggcgcgg ccttgacata
     1441 gccatcaccg catgcccggt accgcaggtg cagtcggcga tgctgccgct gatgaattat
     1501 gggggcaggg tcaatttctt cggagggata ccggcaaaca agcagcccgt tcccatcgat
     1561 accaatctcg tgcattacag ggagctgtac ctgacgggat cgaccaggtc cagcatcgcg
     1621 cagttcagga agactcttga gttcgtatct cagggtctga taaacgttaa tgcaatgata
     1681 acggaccgtt atgaactgaa ggatatcagg gacgcttttg aaaatgtgag ggccgcaaaa
     1741 ggcataaagc atgttataac gttctgaaac atcaggattt gatacgcagc agtgaattca
     1801 ccaatattta cagtttgttt atgtaccaat taaatgtacc aggagggatg gcaaatgaaa
     1861 gtacgtctgg ttccattgca tttcgctgaa gcaaatgaac gagaaagaca ggaattcaag
     1921 gatcagatgg cgcgtctccg cgattattac ggagatgtgg cggaattcct ccctgaagtg
     1981 tgcgtgggag atcctctgcc cgaagcggac gccattgttt ttccgcagtt gatatttgcg
     2041 gcatttcgcc acaatgatga aatgaaagcg tatgatttgc ccatgatcgt gcttacgtcc
     2101 aggttcggca ctgttgagat gtgggactgg gagatcgtca cttatttgcg cgacctgggc
     2161 tgcacagtat tttcgcccta cagcattgaa atggcaaaaa cgatcctcag ggctatcgct
     2221 gtcaggcaca gcctgaagca aggggccagg ttcctgatgt tccaggattc tcccggcgaa
     2281 ggcatgcagg caaacatatt caagcgcttt tactggtggg agaaggaaag caccgaaacg
     2341 atggaggaag tattcggctg caaactgata taccggagct ggaaggaagt aggcgaccgc
     2401 gcgaagcaga tttcagacga ggaagcattg aagataagcg aggactggga aataaaccag
     2461 gaaggcgtaa gccggatggc gttcctgcgc gctgtaaaga tatatattgc cgtaaaggaa
     2521 gtcatcagcg agataggaga tgtggcagga gtcggctcca actgcctgaa cgagtcgttc
     2581 aactgcgaca ccacgccgtg ccttgcgtgg aacatgttgt tcgaacgtga caatataatc
     2641 tgggcgtgcg agggagatac gctgacgctt ctctcgactt atataatata ccgctccatc
     2701 gaaaagccca ttatgatgac gaacctgtat ccattcctgg tcggaatggc ggcactggcg
     2761 catgagaaga tcgacaggtt ccctgatata gaggatcccg acaaccatgc actgggagtc
     2821 cactgcggct acttcgggtt tgctccgagg aacttctgca cccagtggac actgcggccg
     2881 aaggtcctgg aaatcgtcaa tgacgacgcc atcgttatag actgttgctt ccccatgggg
     2941 aaagtaacgc ttgccaagat ttatcccggt tttaagaaac tgagcgttat tgaggcggaa
     3001 atagaggact atgtccagta tccgggttcc gactgcagga acggcgcatt gatacgctac
     3061 aaggacgggc acagggtcat ggacgagctg tgctcgcacc attcccttat aatagccggg
     3121 gatgtaaggc cgcagctgag gcagcttgca aaggtcttcg gtttcagcgt tactgagctg
     3181 tgatcaggga cgttcgatat tcatgatata aatctattgt caattggaat tatggcttgt
     3241 ttcattgtca tgcttccata acaggcattc agaccgatga tgtgccccgg tacggcgcca
     3301 ggcggataca gacctgaagg tgccgcacgg ggcatgcttt catcgtttcg ttttaaaata
     3361 gtgtttaccg gataaagtaa gcggatccgc gtttattcgc atttctaata tttttatttc
     3421 ttgttatcat ttcctttctt ttaggtgcaa attgcataaa atttattatc gatttttaat
     3481 gaaatttatc gtttattatt gacacgcgac aaatctgctg atatattatt ttatgtgaaa
     3541 tgattataac aagttgataa acagcaggtt tgcaataatg ttttgaattt ccaaaaggcg
     3601 caacaacagc ggttcacagc agcaacggac atagaaacaa atacagcaag taacggggta
     3661 aatgttatga tgattggtgc aagctcttat atctgggtgt cccctttttc aacagaagaa
     3721 ttggaacaac tgtcgcttgc aaaaggacta gggttcgacg tatatgagat cgctgtggaa
     3781 aggcctgagc tgatagatga tgagaaggtg ctcgccgagg cgaatcgggc aggtataaaa
     3841 gtgcatgtct gcggcgctta tggcgagacc agggacatca gcagcgatgt gcctgggtac
     3901 cgtgaaaacg gtatggccta tgggaagcgc cttatcgata tggcggccgt actgggctcg
     3961 ccttatgtgg cgggcccgat gttcgcggct gtcggaagga cacgccttgc ggacaccggg
     4021 gaaaaacagc gccagatgga ttatgctgtt gaaaatatga agcgccttgc cgggtacgcc
     4081 gcgaacaggg gcataaagct ggcgatcgag ccgctgaacc ggtttgagac cgattttata
     4141 aataccgtcg aacagggcct gtattttatc gacaggatcg gatacgacaa tgtaggtttt
     4201 cttctcgata cattccacat gaacatcgaa gagaaatcga taccttgcgc tattcgttcc
     4261 gcaaagggaa ggatattcga tttccatgcc tgcgcaaacg accgcggcac tccgggggag
     4321 gaccacttcg actgggagga gatccgcaaa gcgctggacg atgctgatta taaagggccg
     4381 gtcgttatcg agtctttcac gcctaatata accgaaatcg cg
//
LOCUS       DAOSCC010000198         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12447_length_4422_cov_4.136020, whole genome shotgun sequence.
ACCESSION   DAOSCC010000198 DAOSCC010000000
VERSION     DAOSCC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12447_length_4422_cov_4.136020"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLG72_09510"
     CDS             <1..219
                     /locus_tag="PLG72_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOJ81071.1"
                     /translation="IMDAASERVRLADAEGRTSKCIIAPYPPGTALICPGEEITREAV
                     DFLADVIMAGGSVHGVDSDGTVLVLCRK"
     gene            304..942
                     /gene="tmk"
                     /locus_tag="PLG72_09515"
     CDS             304..942
                     /gene="tmk"
                     /locus_tag="PLG72_09515"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ81072.1"
                     /translation="MKRGLFVTVEGTDGCGKTTQIRNIIDHLSSMGCKVVVTREPGGT
                     RISESIRSIILDPAYTEMHSKTELMLYSAARAQLVEQVIKPAIANCKTVICDRYVDSF
                     YAYQGYGRGLDMDMLKQITSIAIDNTMPDITFFFDLDPEIGLRRRMNAANGDRIENEQ
                     LDFHRRVYNGYKQLAKENPDRIKTIDASRSVEEVWKDVRRQLDTVLGFVREV"
     gene            977..1306
                     /locus_tag="PLG72_09520"
     CDS             977..1306
                     /locus_tag="PLG72_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ81073.1"
                     /translation="MKLVFAIVHDEDGPKVMEELNRNGFSVTKLCSTGGFLKAGNTTL
                     LVGVDEEHLDNVIEVIKKKSKSRKQVINSNMTPNGVGGMFIPYPVEVVVGGATIFVLN
                     VDRFEKA"
     gene            1340..1792
                     /locus_tag="PLG72_09525"
     CDS             1340..1792
                     /locus_tag="PLG72_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOJ81074.1"
                     /translation="MKVSETLTNTAGLAGLPVKDDKKVKPGKRGEFRHQLKMAEDAGY
                     EQYLKDLVDEIARQGERLAERVDIRELKIYKKLIAEFLDLAVGKSKKFSKRNLLDRRG
                     RHRVFAIVKNINEEVEQLTQDVLNGEKDNLKLLQRLADIRGLILDLFT"
     gene            1889..2866
                     /locus_tag="PLG72_09530"
     CDS             1889..2866
                     /locus_tag="PLG72_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HOJ81075.1"
                     /translation="MDFEGIAGQRDVIENLKRSLSEDRVSHAYIFSGPQGIGKKTIAR
                     IFAGLLLCGDPHDGATCGRCKACLLMENGTNPDLVTIKTDGPSIGVDQIRGIQDDAVL
                     RPMYSKRKVYIVEDAAKMTVQAQNCLLKTFEEPPGYVVVILLTANYESLLETVRSRAL
                     HLQFRKYTHDQVMQALRTRIGGSSTMLELIAGYADGNIGQALKLAESSEFSALRDRLF
                     DLLPGAASGKTKDIFEITAFLEDNRENIDVLLDIMLVYYRDLLVAREGGESMLINSDK
                     KDIIMNNAQNYRDSRIIECIDAIEAARGALRQHANYQLATDYLLIKLQG"
     gene            2901..3779
                     /locus_tag="PLG72_09535"
     CDS             2901..3779
                     /locus_tag="PLG72_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOJ81076.1"
                     /translation="MVKVVGVRFKRAGKIYYFDPDEFQIEKGMHVIVETARGVELGEV
                     VIPPREVPDEEIVAPLKKVMRIATEDDEKHAQENRKKEKDAFGICLQKIRDHNLEMKL
                     IDVEYTFDNNKILFYFTADGRVDFRELVKDLASVFKTRIELRQIGVRDESKMMGGIGV
                     CGRELCCCSHLGEFQPVSIKMAKEQGLSLNPTKISGNCGRLMCCLRYEQEAYEQIIKY
                     APRIGAVVETPDGQGVVIERFLLKETAKVKLDKGNETDLRSYRFSELKVIQDASAQEE
                     NDVEDAEIEELKALED"
     gene            3895..4065
                     /locus_tag="PLG72_09540"
     CDS             3895..4065
                     /locus_tag="PLG72_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ81077.1"
                     /translation="MAYRISEDCISCGQCESECPVSAISQGDGKYEIDEDACIDCGTC
                     AEVCPVEAPKPV"
     gene            4209..>4422
                     /locus_tag="PLG72_09545"
     CDS             4209..>4422
                     /locus_tag="PLG72_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOJ81078.1"
                     /translation="MDIEIKENERIDDLQYKGLKLIQKKDGFCFGIDAVLLSYFAQVP
                     KSGRVIDLGTGTGIIAVLVAAKKEPAG"
BASE COUNT         1280 a          928 c         1283 g          931 t
ORIGIN      
        1 ataatggacg ctgcttccga gagggtcagg ctggcggacg cggaaggaag gacaagcaag
       61 tgtataatag cgccataccc gcccgggaca gccctcatat gccccggtga agagatcacg
      121 cgggaagccg tggattttct ggcggatgtg atcatggccg gcggcagtgt ccatggagtg
      181 gacagcgatg ggacggttct ggttttatgc aggaaataaa tctaatatgg taacgtatgc
      241 tgtaataggg tataatttaa gtaggaaacc ggagcagcgc ggaaaagttg ggggatggga
      301 gtcatgaaaa gagggctttt cgtcactgtg gaaggcaccg acggctgcgg aaagacgacg
      361 cagatcagaa acataataga tcatctttct tccatgggat gtaaagtcgt tgtgacaaga
      421 gaacccggag ggacgcgcat cagcgagagc atccgttcca tcatcctgga tcctgcctac
      481 accgaaatgc acagcaaaac ggaactgatg ctgtattcag ccgcccgcgc acagttggtc
      541 gagcaggtga tcaaacccgc catagcgaac tgcaagacag tgatatgcga ccggtatgtg
      601 gattcatttt atgcatacca gggctacgga agaggacttg atatggatat gctgaagcag
      661 ataacaagta tagccataga caatacgatg cccgacatca cgtttttctt tgacctggat
      721 ccggagattg gtctcagaag aaggatgaat gccgcaaacg gcgacaggat agaaaacgaa
      781 cagctggatt tccaccgaag agtctataac ggttataaac agcttgcgaa agaaaaccct
      841 gacaggatca aaaccatcga tgcttccagg tcggtggaag aggtctggaa agacgtcagg
      901 aggcagttgg atacagttct tggctttgtc agagaggttt gatgcgatgc atgtattttg
      961 ttgaggaggg ctgaatatga aacttgtgtt tgctatcgtc catgacgaag acggcccgaa
     1021 ggtgatggaa gaactgaaca ggaacggttt cagcgtgacc aagctctgtt ccaccggagg
     1081 gtttctcaaa gcggggaaca cgaccctgct cgtaggtgtc gatgaggaac acctggataa
     1141 tgttatcgag gtcatcaaga aaaaatccaa aagcaggaag caagtcatca attcaaatat
     1201 gacccccaac ggagtgggag gaatgtttat accatacccg gtggaagtag tcgtcggggg
     1261 cgcgaccatt ttcgtcctga atgtggatcg gttcgaaaag gcatagatcc gcgatgaagg
     1321 atcctgcggg aggtaaatct tgaaagtaag tgagactctc accaatacgg caggtctggc
     1381 aggcctgccc gtcaaagatg acaaaaaagt aaaacccggc aaacgcggcg aattcaggca
     1441 ccagcttaaa atggctgagg atgccggtta tgaacagtat ttgaaagacc ttgtcgatga
     1501 aattgccagg cagggcgaaa gacttgcaga aagagtagac atacgcgaac ttaagatcta
     1561 taaaaagctg atagccgaat ttctcgacct ggccgtcggc aaatcgaaaa aattctcaaa
     1621 aaggaacctg ctggacaggc gcggcagaca cagggtcttt gcgatagtca agaatataaa
     1681 tgaagaagtg gaacagctta cacaggatgt gctgaacgga gaaaaggaca atctgaaact
     1741 cctccagcgt cttgcagata tcaggggact gatactggat ctgttcacat gatccgcagc
     1801 cggccaaaat ccggttaccg tgagagtata acaactcatg gcatgctccc gggtgcggcc
     1861 ggatcatcat gttatcaagg agtaatgaat ggatttcgaa ggcattgcag gtcagcggga
     1921 tgtgatcgaa aatctgaagc gatcgctttc cgaagacagg gtcagtcatg catatatatt
     1981 ctcaggaccg caggggatcg gcaagaaaac catagcaagg atcttcgcag ggcttttgct
     2041 gtgcggcgac ccgcacgacg gggccacatg cggcaggtgc aaggcctgcc ttcttatgga
     2101 aaacggaacc aatcctgatc ttgtcacgat caaaaccgac ggtcccagca ttggcgtgga
     2161 tcagatacgc ggcatccagg acgatgcggt gctgaggccc atgtattcca aacgcaaggt
     2221 atatatcgtg gaagatgccg caaagatgac tgtccaggcc cagaactgcc tgctcaaaac
     2281 gtttgaggag cctccgggat atgtcgtcgt tatcctgctg actgcaaatt atgaaagcct
     2341 gctggagacg gtacgttcac gggcgctgca tctgcagttc aggaaatata cccatgacca
     2401 ggtgatgcag gcgctcagga caaggatagg cggcagcagc actatgctgg aactgatagc
     2461 cggctatgct gacgggaata tcggacaggc cctgaaactg gcggagtcca gcgaattttc
     2521 cgccctgcgg gatcggctgt tcgatctgct gcccggggcg gcatcgggaa agacaaaaga
     2581 catctttgag attacagctt tcctggaaga caacagggaa aacatcgacg tgctgctgga
     2641 cataatgctg gtatactacc gtgatctgct cgtcgcgagg gaaggcggcg aaagcatgtt
     2701 gataaattcg gataaaaaag atattataat gaataatgcc caaaattacc gcgacagcag
     2761 gatcatagag tgtatagacg ctattgaggc cgccagggga gcattgaggc agcatgcgaa
     2821 ttaccagctt gcaacagact atttgctgat aaagctgcag gggtagaaac tgcagggaca
     2881 aaaccacagg aggacattgg atggtaaagg tagttggagt caggtttaaa agggcaggga
     2941 aaatctatta ttttgaccct gatgaatttc agatagaaaa aggcatgcac gtcatcgtgg
     3001 aaaccgccag gggcgtggaa ctcggcgagg tggtgatacc gccgcgcgaa gtccctgacg
     3061 aggagatcgt ggcgccgctt aaaaaagtta tgaggatcgc cacggaagat gacgagaagc
     3121 atgcccagga gaacaggaaa aaggagaagg acgcttttgg catatgtctt cagaagatca
     3181 gggaccacaa ccttgagatg aaactgatag atgtggagta cacgttcgac aacaataaga
     3241 tcctgttcta tttcactgcc gacggaaggg tcgacttcag ggaactggtg aaggatcttg
     3301 cttccgtttt caagacgagg atagaactgc ggcagatagg cgtcagggat gagtcgaaga
     3361 tgatgggcgg catcggcgta tgcggaaggg agttgtgctg ctgctcgcat cttggcgaat
     3421 tccagccggt atcgatcaaa atggcaaagg aacagggcct ttcgctgaat ccgaccaaga
     3481 tctccggcaa ctgcggaagg ttgatgtgct gcctcaggta tgaacaggaa gcgtacgaac
     3541 agataataaa atacgcgccg aggatcggtg ctgttgtcga gacgcctgac ggccagggag
     3601 tcgtgatcga aaggttcctt ttgaaagaaa cggcgaaagt aaaactcgac aagggcaacg
     3661 aaacagacct gagatcgtac aggttcagcg aactcaaagt catacaggat gcttccgccc
     3721 aggaggaaaa tgacgtcgaa gacgcagaga tagaagaact gaaagctttg gaggactgaa
     3781 tccgctgaaa taaccgaatt atcgacaaaa gtattttctt tatttgtctt tggtgataaa
     3841 atagtaactg tttgttagtg ggttaccaaa atgtatttta ggaggtgtac tctgatggca
     3901 taccgcatca gtgaagactg catcagctgc ggacagtgtg aatccgaatg cccggtttca
     3961 gcaatttccc agggagacgg caaatatgag atagacgagg atgcatgcat cgattgtgga
     4021 acatgtgcag aagtctgtcc ggtcgaagct ccgaagccgg tataacaagt tgggccgata
     4081 aggggtcagg cagcagcttc catgctgatg cagggctcct tattttattt ttgaggagga
     4141 attgacctgg cccgggttgg atttttcgac tgttcctatt ctgagacaga atatgaaggg
     4201 tggagccgtt ggatattgag ataaaagaaa atgagaggat agatgacctt cagtataaag
     4261 gcctgaagct gatacaaaaa aaggacgggt tctgtttcgg catcgacgcg gttctgctgt
     4321 cttactttgc acaagtaccg aaaagcggca gagtgatcga tcttggtaca ggaaccggga
     4381 tcattgcggt gctggtagcc gcaaaaaagg agcccgccgg cg
//
LOCUS       DAOSCC010000199         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12524_length_4398_cov_3.782408, whole genome shotgun sequence.
ACCESSION   DAOSCC010000199 DAOSCC010000000
VERSION     DAOSCC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12524_length_4398_cov_3.782408"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLG72_09550"
     CDS             <1..249
                     /locus_tag="PLG72_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ81079.1"
                     /translation="LYMAGLYKPWDYARTMGASAIHPLFYNIVPEVVRGCKENGIMIN
                     PFTVDRPEHIKAVAEAGVDGIITNVPDVALKIVKEMGA"
     gene            251..1483
                     /locus_tag="PLG72_09555"
     CDS             251..1483
                     /locus_tag="PLG72_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ81080.1"
                     /translation="MKLNYRQTMLIGLGFFTVSIVWGIYNIAMPIYLDDLGLSGLAVG
                     AVMTIDNIFAVIFLPIFGALSDRTNTRYGRRMPYLLVGIPLSALAFILIPFLRMSLIP
                     LMITVIVMNFAMSIYRAPTVALMPDFTPGPLRSKANGVINFMGGVGAVMAFLIGGFLF
                     RLNEYLPFAVCSGIMMLTIIVMYLKIREPKEIADAKPGAPDEETSSEKKEKGVMLSLV
                     FLLLAIFFWFIAYNAVETFFSTFGEKVLGIDKSQSSFMLSVFSAMLVIFSIPSGFIAS
                     KIGRKRTILIGITMLFLVFVTMNFTMGMDPYIIYILLAVGGISWALININSYPMVVEM
                     TTSKGIGKYTGYYYFFSMTAAILSPVLFGFIKDILGDSFLFIYSSIAMVLAFFFMMLV
                     KHGEPEGKPKASLQEEAR"
     gene            1573..2139
                     /locus_tag="PLG72_09560"
     CDS             1573..2139
                     /locus_tag="PLG72_09560"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ81081.1"
                     /translation="MLSWEELEKSCRECTRCGLAKTRTNVVIGRGSKTAPVMLIGEGP
                     GEQEDLQGKPFVGAAGQLLDLLLSALMFKEDDYYICNIVKCRPPGNRIPTDDEAESCL
                     PWLRNQTLLVRPRIMVCLGATAMKYIIDRNAKISQIRGQWIERKGFWIMPTFHPAALL
                     RDTTKKPLMFEDMKKVKLKLDELRSPQY"
     gene            2217..2819
                     /locus_tag="PLG72_09565"
     CDS             2217..2819
                     /locus_tag="PLG72_09565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ81082.1"
                     /translation="MRKDESLSLLYNEYKCRFPGKEYVFGCGCTSPKLLLIGEAPGRE
                     EVKFSRPFSGAAGKWLDSFLQLAGINREDIYITNAIKYRLSKENPKTGRLSNRPAARE
                     EIVSSRPYLLDEIGIICPCCIVTLGNVPLRAVTGDHRLNIGECHGTPSKVSIKERDYL
                     LFPLYHPASVIYNRDLVQTYESDLRGLVTLLKAVKGHSSA"
     gene            complement(2868..3881)
                     /locus_tag="PLG72_09570"
     CDS             complement(2868..3881)
                     /locus_tag="PLG72_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ81083.1"
                     /translation="MVINMREWVRNIIGSEKRYSMPIMTYPGLALSGKTIPEVIRSGE
                     DQFSCIYALAQKYPSAAAMTIMDLSVEAEAFGSPVRYSDNEVPTVSGNIVSDDESARE
                     LKIPQVGTGRTSSYIKAAALSAQNISDRPSFGGQIGPFSLACRLYDMTEIMVALFDEP
                     ETVHIVLEKAAEFLVSYAKAFREAGAAGIIIAEPAAGLLSPEQCAEFSSPYVKKIVDA
                     VQDDNFMVILHNCGNTVKLVPSMLTTGAMGFHFGNAVDMEDILPQIPGDRLAFGNIDP
                     ARVFRNGTPEDVREKTWDLLNRTAGYRNFVISSGCDIPPGTPIENIEAFFETVERYNK
                     ENT"
BASE COUNT         1064 a         1055 c         1137 g         1142 t
ORIGIN      
        1 ctttatatgg caggcctata taaaccgtgg gactatgcgc gcaccatggg cgcatcggcg
       61 atacacccgc ttttttacaa tatagtgcct gaagttgtca ggggatgcaa agagaatggt
      121 atcatgatca atccatttac agtggaccgg cctgagcaca taaaggcagt ggcggaagcg
      181 ggtgtcgacg ggatcataac caatgtgcct gatgttgctc tgaaaatagt gaaggaaatg
      241 ggggcgtaat atgaaactga attacaggca gaccatgctc atcggacttg gattcttcac
      301 agtcagcata gtttggggga tatacaatat cgcgatgccg atctatcttg acgacctggg
      361 tttgtccggg ctggctgtgg gagctgtgat gacgatagac aatattttcg ccgtgatatt
      421 cctgccgatt ttcggcgccc tgagcgacag gaccaatacc aggtacggaa ggaggatgcc
      481 gtacctgctc gtgggcatac ctttatcggc cctggccttc atccttatcc cgtttttgag
      541 gatgagcctt attccgctga tgataacggt aatagtgatg aacttcgcca tgagcattta
      601 cagggcgcct accgttgcac taatgcctga tttcacaccg gggccgctgc gcagcaaggc
      661 caacggcgtc atcaatttca tgggcggcgt gggggccgtc atggcgttcc tgataggcgg
      721 ttttctcttc aggctcaatg agtaccttcc ttttgcggtc tgctcgggga taatgatgct
      781 gacgatcatc gtcatgtatc tgaaaatccg tgaaccgaag gagatcgccg atgctaagcc
      841 tggtgctcca gatgaggaga cttcatcgga aaagaaggaa aaaggcgtga tgctgagcct
      901 cgtgttcctg ctcctggcca tattcttctg gttcattgcc tataatgccg tagagacttt
      961 tttctcgacg ttcggggaaa aggtgctcgg gatagataaa agccagtcgt cattcatgtt
     1021 gtccgtgttc tccgcgatgc tggtcatttt ctccatacct tcggggttca tagcctcaaa
     1081 gatcggaagg aaaaggacca tactgatcgg gatcacgatg cttttccttg tgtttgtaac
     1141 gatgaacttc acgatgggga tggatccata cataatatat atcctgcttg ccgtcggcgg
     1201 tataagctgg gccctgataa acatcaactc ataccccatg gtggttgaga tgactacaag
     1261 caagggcata ggcaagtata ccggttacta ttactttttc tcgatgacgg cagccatact
     1321 gagccccgtg ctgttcgggt ttatcaagga tattctgggc gacagtttcc tgttcatcta
     1381 ttcgagtata gcgatggtgc ttgcgttttt cttcatgatg ctggtaaagc atggggagcc
     1441 ggaagggaaa ccgaaggcgt cgctgcagga agaagcccgg tgatcctgtc gggtgtaaga
     1501 tatttgggac caatgcatgg atgatatctg cttattttag aatttacatg tgatccaggg
     1561 ggtaatgatc ctgtgctgag ctgggaagag ctcgagaaga gttgccgtga atgtacgcgg
     1621 tgcggccttg ccaaaacaag aacgaacgtc gttatcgggc gcggttcgaa aaccgccccg
     1681 gtcatgctga tcggagaagg tccgggcgaa caggaggacc tgcagggaaa acctttcgtc
     1741 ggcgcggcag gacagcttct cgatctgctc ctgtctgcgc tgatgttcaa agaggatgat
     1801 tactacatat gtaatatcgt gaagtgccgg ccgcccggaa accggatacc aaccgacgat
     1861 gaagcggaaa gctgcctgcc ctggctccgg aaccagacgc tgctggtaag gccgcggatc
     1921 atggtgtgcc ttggcgcgac tgcgatgaag tacatcatcg acaggaacgc aaaaatatca
     1981 cagataagag ggcagtggat agagcgcaag ggattctgga taatgccgac tttccatccg
     2041 gctgcgcttc tcagggacac gacgaagaaa cccctgatgt tcgaggatat gaagaaagtg
     2101 aaactcaagc ttgacgaact ccggagcccg caatattgac ggatgatctg ctgcggccgg
     2161 atacagccgc gttttcacgg tccaacactt tatgatggtt tgcaggagag aaacacatga
     2221 gaaaagacga gagtttatct ttattatata atgaatacaa atgcaggttt ccggggaaag
     2281 agtatgtttt cggatgcggc tgcacgagcc cgaagctcct gctcataggc gaagcaccgg
     2341 gacgtgaaga ggtgaagttt tccaggccgt tctccggcgc tgccgggaaa tggctggaca
     2401 gtttcctcca gcttgccggg ataaacaggg aggatattta cataaccaac gccatcaaat
     2461 acaggctttc aaaagagaac cccaaaacag gcagactgtc gaacaggcct gcggccaggg
     2521 aagagatcgt atccagcagg ccgtatctgc ttgatgagat cgggatcatc tgtccctgct
     2581 gcatcgtaac tcttggcaac gtcccgctaa gagcagtgac gggggatcac aggctgaata
     2641 tcggcgaatg ccatggcacg ccttccaaag tgagcataaa agagcgggat tacctgctgt
     2701 tcccgctcta tcatcctgcc agcgtcatct acaaccgcga tctggtgcag acatatgaat
     2761 cggatttaag aggtctggtg acactcctga aagcggtaaa gggacactcc tctgcatgat
     2821 ggattccatc agggtcgagg atgtcccttg aaagcagctc ccgggtgtca tgtattctct
     2881 ttgttatatc tctcgactgt ctcgaagaat gcttcgatgt tttctatcgg tgttcccgga
     2941 gggatatcgc agcctgatga gatgacgaaa ttcctgtatc cggctgtcct gttcagcaga
     3001 tcccatgttt tctcccgtac atcttccggc gtaccatttc tgaataccct tgccgggtct
     3061 atattgccaa atgccagcct gtctccgggg atctggggca atatatcttc catatcgact
     3121 gcgttcccga agtggaatcc catcgcgccg gtggtaagca tcgaaggaac cagctttacc
     3181 gtgttcccgc aattatgcag tatcaccatg aagttgtcgt cctgtacggc atcgactatc
     3241 ttctttacat atggtgaaga gaattccgca cactgctccg gcgacagaag ccctgccgca
     3301 ggctcggcaa tgattattcc ggccgctcct gcttccctga atgcttttgc atatgatacg
     3361 aggaattctg ctgctttttc gagcacgata tgtactgttt ccggttcgtc aaaaagggct
     3421 accattattt ccgtcatgtc ataaagcctg catgccagcg aaaaaggccc tatctggccg
     3481 ccaaaggatg gcctgtcact gatgttctgc gcactgaggg ctgcagcctt tatataagag
     3541 cttgtccttc ctgttccgac ctgcgggatc ttcagttctc tggctgattc atcatcgctc
     3601 actatattcc ctgaaactgt cgggacctcg ttatccgagt acctgaccgg acttccgaaa
     3661 gcttcggctt cgacggacag atccattatc gtcattgccg cggcggacgg atatttctgt
     3721 gccagcgcat aaatgcagct gaactggtct tcgccgctcc tgatcacttc gggtatggtc
     3781 ttgcccgaaa gcgccagtcc gggatatgtc attatcggca tcgagtatct tttttcagat
     3841 ccgattatgt tgcggaccca ttccctcatg ttgatcacca tgtcaaaact cctttttagt
     3901 aatatttatg tgtgacattt ctcgttctca aaagaatact tcttgcaggg gagtatcacc
     3961 ataccttttt catgtgacat tcctcgtctc caaaggaata ctttttggtg acattcctca
     4021 tcccaaaagg aatgcttcct gttgaggagt gtccctttac cgcattcgag gagtgtcacc
     4081 ataccgttct cttcgcacct aattctacaa caatcgggaa acagtttata taaatagatt
     4141 tcaaagaata gtactatttt attatcttac gttctatgct aaaatacttt atgaaccaat
     4201 tgtcgagtga atttgtatcc catcggccgg aaaaccggcg tatttttgga aatcgtgaaa
     4261 taatacaaaa tattaaaaaa ttgttcaata ccaggcatgc attaaatagt atgattttat
     4321 ttggactggc ggagccggca gatccaatgc tcatccatac ctggtacggc ggcggtcagg
     4381 actgccgttg acgcggaa
//
LOCUS       DAOSCC010000200         4389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12556_length_4389_cov_4.279649, whole genome shotgun sequence.
ACCESSION   DAOSCC010000200 DAOSCC010000000
VERSION     DAOSCC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12556_length_4389_cov_4.279649"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..747)
                     /locus_tag="PLG72_09575"
     CDS             complement(133..747)
                     /locus_tag="PLG72_09575"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ81084.1"
                     /translation="MWYQQRQKQHDWEIAPDILDDRYSLLDALVVGGLGVTLLNNSDR
                     VKIACLAQLVNVIAPIITEKNGRVIRQSIYYPFRDISKYGRGTVLKPVVITPSVETCH
                     GDAPLLHISVVYNEENGYITVFCLNIDQSGDMLLELDMRSFGKLSMAEHICLRGDDLN
                     AVNTFDAPDTVIPESMGIIGGEHETFSVRIPKLSWNVLRFSAKG"
     gene            748..1074
                     /locus_tag="PLG72_09580"
     CDS             748..1074
                     /locus_tag="PLG72_09580"
                     /inference="COORDINATES: protein motif:HMM:NF027687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ81085.1"
                     /translation="MIGRYRKNDVAGLYKLVCHGDDITKEIKTSVPVELGKDGSVSGS
                     YSGKWRLSSGNKIEIELDGTVYKGVVLKQRDTDNMKMVFTFTALSDSGTAIWGSKAII
                     QDRTLN"
     gene            complement(1233..1928)
                     /locus_tag="PLG72_09585"
     CDS             complement(1233..1928)
                     /locus_tag="PLG72_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOJ81086.1"
                     /translation="MLEALKEQVLQANLDLVRHGLVVFTWGNVSGIDRERGLMVIKPS
                     GVPYDEMKPEHMVVLDLDGKKVEGDLRPSSDTPTHIAIYKAFAGVGGVVHTHSSMATS
                     WAQAGRSIPALGTTHADHFYGEIPCTRKLTKEEIDEDYESNTGKVIVETFADIDPLHV
                     PGVLVNGHGPFSWGKDAHDAVVNAVVLEEVAKMAFNTFVINPDIRPIDGWLLDKHFLR
                     KHGPKAYYGQKKP"
     gene            complement(2085..3764)
                     /gene="araB"
                     /locus_tag="PLG72_09590"
     CDS             complement(2085..3764)
                     /gene="araB"
                     /locus_tag="PLG72_09590"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOJ81087.1"
                     /translation="MSNKKYSLGIDFGTQSGRAVLVETDTGNIAATAVKEYTHGVMDE
                     YLPDGKTRLEPDWALEHPADYLEVLAVTIPAVLKEAGVSPEDVIGIGIDFTACTMLPI
                     KSDGTPLCFLDEFKQNPHAYVKLWKHHAAQPEANRLNEIAETMGFDFLKRYGGKISSE
                     WLIPKIMQILDEAPEVYEAADKFIEATDWIILKLTGNERRNSCTAGYKAIWSKREGYP
                     PKEFFKALDPRLENLVDEKLSRDIYPLGGKAGELTKEMAELTGLLPGTAVAIANVDAH
                     VAVPAVGIVEPGKMLMIMGTSTCHMVLGTEERIVPGMCGVVEDGIIPGFLGYEAGQSC
                     VGDHFEWFIENCLPASYKEEAKQKGVNIHKFLREKAKKLRPGESGLIALDWWNGNRSV
                     LVDADLTGVLLGCTLNTKPEEIYRALIEATAYGTNMIIETFESNGVPIKELYACGGIA
                     EKDEFMMQIYADVTNREIRISASPQTVALGSAMFGAVAAGKAKGGYDSIFDAAKRMSK
                     VKDDYYKPIPENVEIYKKLYAEYKILHDYFGRGANDVMKRLKEIKKSVSEN"
     gene            complement(3931..>4389)
                     /locus_tag="PLG72_09595"
     CDS             complement(3931..>4389)
                     /locus_tag="PLG72_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ81088.1"
                     /translation="DDIQGINRHKGYARALADGGIAYDPGKVVWFHTEDRDTKPGAAI
                     RDIIAGGVRVDAVAAYNDEAAVKVILQLKEMGLRVPEDISVVGFDDSYLALNGPVKLT
                     TVAHPKEKLGEAAAELLLELMSGADLPGDAVEKVIVPGLIVRDSCMPRHG"
BASE COUNT          968 a         1218 c         1032 g         1171 t
ORIGIN      
        1 tatatttcca ttaacatggg ttattgctgt ataattatgt caaaaagcgg tcggagttca
       61 ttacgatccg accgctttcg atccggtatc ttttgatcag tcaaatgtgt atcctgtcct
      121 tcatccgaca tatcatcctt ttgcactgaa acgcagaaca ttccaggaca gcttcggtat
      181 acggacactg aaagtttcat gttcgccgcc gataattccc atgctttcgg ggatcacagt
      241 atccggagcg tcgaaagtgt tcacggcatt gagatcgtct cccctaagac agatgtgctc
      301 tgccatcgag agtttcccaa atgaacgcat gtccagttcg agcagcatat cgccgctttg
      361 gtctatgttc aggcagaaga ccgtaatgta tccgttttct tcattatata caacgcttat
      421 atgcagcaga ggcgcatcgc catggcacgt ctcaaccgaa ggcgttatta cgacaggctt
      481 gagtacagtg cctcttccat atttcgaaat gtccctgaaa ggataataaa tgctctggcg
      541 tatgacacgt ccattttttt cagtgataat cggcgcaatg acattcacca gctgcgcaag
      601 gcatgcgatt tttaccctgt ccgagttatt caggagcgtc actccgagcc cgccgacaac
      661 aagagcgtcc agcagcgaat acctgtcgtc gagtatgtcg ggagcgattt cccagtcatg
      721 ttgcttctgg cgctgctgat accacacatg atcggcaggt acagaaagaa cgatgttgca
      781 ggcttgtaca aactggtttg ccacggtgac gatataacga aagaaataaa aacctcagtg
      841 ccggtcgaac tcggcaaaga cggaagcgtt tcaggcagtt acagcggaaa atggagactg
      901 tcatccggca acaaaattga gatagagctt gatggtaccg tttataaagg ggtagtactg
      961 aagcagcggg atactgacaa tatgaaaatg gttttcacgt tcacggcgct ttcagattca
     1021 ggaaccgcaa tctggggcag caaagcaatc atacaggaca gaacgcttaa ctaacttcca
     1081 ttgtgccata aaagacttta ttagtaaaag ggcactgcaa ccgttttaat atgcagtgcc
     1141 ctttatgttc aggtccaccc gggcctatgt gattttccgg gatgaaaacc tgtatcctga
     1201 aaggatccgt aaggatctgc tgccgttcag ccctatggtt tcttctggcc gtaataggct
     1261 ttggggccgt gcttcctgag gaaatgcttg tcgagcagcc agccgtcgat cggtctgatg
     1321 tccggattga tgacaaaagt gttgaacgcc atctttgcga cttcttccag cacaacggca
     1381 ttcacgacgg catcatgggc gtctttgccc cagctgaacg ggccatggcc gttgaccagg
     1441 actccgggca catgcagcgg gtcgatatct gcaaaggtct cgacaataac ctttccggtg
     1501 ttgctttcat agtcctcgtc gatctcttcc tttgtcagct tcctggtgca gggtatctca
     1561 ccgtagaaat ggtcggcatg ggtcgttcca agcgccggta tgctcctccc tgcctgggcc
     1621 cagcttgttg ccatggagga atgggtatgc accactccgc cgactccggc aaacgcttta
     1681 tatatcgcta tatgtgtcgg cgtgtcggaa gaaggtctca ggtctccttc gacctttttc
     1741 ccgtcaaggt ccagcacgac catgtgttca ggcttcatct cgtcatatgg aacgccgctc
     1801 ggcttaatga ccatcagccc gcgctccctg tcgatgccgc tgacattgcc ccacgtgaat
     1861 acgacaaggc catgcctgac aagatccaga tttgcctgca aaacttgttc ttttaatgct
     1921 tccagcataa tttacctccc tggataaaga atataccggc agcagtcagt actgctgccg
     1981 gacatatatt aagcagttat tgcaaagcag ttgtttcact gatgcctgcc cctgtaattc
     2041 actgatgctg tccttaatat ttcgatgata tttcgatgct gccgttaatt ctccgaaacc
     2101 gacttcttta tctccttcag ccgcttcatc acatcattgg cgcctctgcc gaagtagtca
     2161 tgcaggatct tgtattctgc ataaagtttc ttatatattt ccacattttc aggtatcggc
     2221 ttgtaataat cgtccttgac cttcgacatg cgctttgcgg cgtcaaatat ggaatcgtag
     2281 ccgccctttg cctttcctgc agccactgcg ccgaacattg cggagcccag tgctacggtc
     2341 tgcggagaag ctgaaatcct tatctccctg tttgtcacat cggcgtatat ctgcatcatg
     2401 aactcgtcct tctcagcaat gccgccgcac gcatacagct cttttatcgg tacgccgttg
     2461 ctctcgaatg tttctattat catattggtg ccgtaagccg tagcttctat cagcgctctg
     2521 tagatctcct cgggcttcgt gttgagcgtg cagccaagca ggactcccgt aaggtccgca
     2581 tctaccagca ccgaccggtt gccgttccac cagtcaaggg caatgaggcc gctttcgccg
     2641 ggtctgagct tctttgcctt ctccctgagg aacttatgga tattgacgcc tttttgctta
     2701 gcctcttctt tgtatgaagc gggcaggcag ttttcgatga accattcgaa atggtctccc
     2761 acgcaggact gtcccgcttc gtagccaagg aagccgggga ttatgccgtc ctcgaccacg
     2821 ccgcacatgc cggggacgat cctctcttca gtgccaagca ccatgtggca ggtggaagta
     2881 cccattatca tcagcatctt cccgggttcc acgataccca cggcagggac cgcaacatgg
     2941 gcgtccacat tcgcgatcgc aacggccgtg cccggcagaa ggcctgtcag ttccgccatt
     3001 tccttcgtaa gctcccctgc cttgccgcca agcggataaa tgtccctgct cagcttctca
     3061 tccacaaggt tttcaagcct gggatcaaga gccttgaaaa attctttcgg cggatagcct
     3121 tcacgcttgc tccatatcgc tttatatccg gccgtgcagc tgtttctcct ctcattgccc
     3181 gtcagtttca gtattatcca gtcggtagcc tctataaatt tgtcggccgc ctcgtaaact
     3241 tcaggcgcct cgtcaagtat ctgcattatt ttcgggataa gccattcgga cgatattttg
     3301 cccccgtatc ttttcaggaa gtcaaagccc atcgtttcgg ctatctcatt gagcctgttc
     3361 gcttcaggct gcgcggcgtg atgcttccag agctttacat acgcatgagg gttctgtttg
     3421 aattcatcga gaaaacaaag gggcgtcccg tcggatttta tcggcagcat ggtacaggcc
     3481 gtaaaatcga tgccgatgcc tatcacatcc tcaggtgaaa cccctgcttc cttaagcacg
     3541 gcaggtatgg taacggcaag cacctcaaga tagtctgccg gatgctccag cgcccagtca
     3601 ggttcgagcc tggttttgcc gtcaggcagg tactcatcca ttactccatg ggtgtattcc
     3661 ttcactgcgg tcgcggctat gttgccggtg tcggtctcca ccaatacagc tctgcccgat
     3721 tgcgtaccga aatcgattcc aagagaatac ttcttgtttg acattttaat taccccctat
     3781 cactggatac ttgatacttt gtacttatta tggcataaag ccgcaaaact cataaatccc
     3841 tgtcagccgg actgttacag catggtattc aggacagtgc agaattcagg agcggtgcat
     3901 catccgggtc tgtacggcag gatgtccata tcagccatgc cgcggcatac acgaatccct
     3961 tacgatcagc cccggtacga tgaccttttc aactgcgtcg ccaggcaggt ccgcaccgct
     4021 catcagttcc aggagaagtt ccgcagccgc ttctcccaac ttttccttgg ggtgtgccac
     4081 tgttgtcagc ttcaccggac cgttcaacgc caggtacgaa tcgtcgaacc cgaccacgga
     4141 tatgtcttcc ggcaccctga gtcccatttc tttcaactgc aggattactt tcaccgcggc
     4201 ctcgtcattg tatgccgcga cagcatcgac acggacgcct cctgcgatga tatccctgat
     4261 agcggcgccc ggcttggtat ccctgtcttc agtgtggaac cacactactt ttccaggatc
     4321 gtacgcgatc cctccgtcgg ccagcgctct cgcatagccc ttatgcctgt tgatgccctg
     4381 gatatcgtc
//
LOCUS       DAOSCC010000201         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12624_length_4370_cov_4.568019, whole genome shotgun sequence.
ACCESSION   DAOSCC010000201 DAOSCC010000000
VERSION     DAOSCC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_4370_cov_4.568019"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1176
                     /locus_tag="PLG72_09600"
     CDS             241..1176
                     /locus_tag="PLG72_09600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOJ81089.1"
                     /translation="MVITVTINPAMDIVMKLDDFRLNVTNRIQKKFRCVGGKGTHVSI
                     NLSLLGVKNTATGVVMGSTGEEIIRRLSEHDIDVRFLKLNRGESRTNYVITDSEGNCT
                     LISEPGQQFEKDVVEMFVSHYSELVGKDDMVVISGDASNQKGTGLQDRLINMAVARGA
                     RFCLDASGEYLENGIRKKPFLVKPNLDELGSLFGSEMKTEDEIVAAIRQVSANGAENI
                     VASCGGEGSYALIGGRLYRAVPPAVKAKNTVGCGDALLSGIIAGFEMKLPAADILKKA
                     TAVAAATAMDESTAGFDPSVAAKLEPEVKVEELEL"
     gene            1284..1886
                     /locus_tag="PLG72_09605"
     CDS             1284..1886
                     /locus_tag="PLG72_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ81090.1"
                     /translation="MAIQYYEERKKLAGTMKLLWDRKLTNAAGGNAAMRVAENRIIIT
                     PSMMSEHRHCEIDAEDILVCDYDLNVIEGTGLLSRECKMHALLLKNIPEIGAVIHAHP
                     EYTMVYAAAKKPVPSVTEATQKMGDCLLIKQAKAYSEDLAENVYRFFRDKRELLRKTG
                     LCGILPLHGTVAVGKHLEQAFSVVERVECDAKCNIFGKMI"
     assembly_gap    1961..2060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2259..3038
                     /locus_tag="PLG72_09610"
     CDS             2259..3038
                     /locus_tag="PLG72_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ81091.1"
                     /translation="MLAYERKSRIIEYLEQNGKAEVNVLAKLLGVVPETIRRDLRELE
                     TQGVLMRTHGGAVIEHPKEKEYPVQVRVMKNALEKDMLCRRAAGFISEGDLIFVDNSS
                     TLVNLIKHISDDIHLTILTNSIKVLQEYALFRKDNITMLCTGGVFSRSNLSLSGTLSD
                     SFTREFFPNKAFVSCHGVSAEHGFTDGNLHEVAFRREIISLAEKVYFLVDHSKFGKLG
                     PIKLGGLDICDVFITDKRPPTELEQQLKQSNPDMEIIVCSE"
     gene            3207..>4370
                     /locus_tag="PLG72_09615"
     CDS             3207..>4370
                     /locus_tag="PLG72_09615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOJ81092.1"
                     /translation="MRLEKKVLAFDAGGGSTRISKIGFDGSRFTLEQIARFPNGPVRA
                     GGNLYWDILGIWRDLLDGLRQAVCEDSEIASLGIDTWGNDFVLLDRKGCMLENPYTHR
                     DSRTTGLVGEVGRLIGGWDLYSRNGIQLNRMNTLYQLFSISRQRPYILDIADKFLFLA
                     DLLAYYLTGEMKNEYTMATISQLYSYSMAGWDHELMELLGIPARIFCPVIRPGEIIGS
                     IYGKVCREAGIPPLRLTAVASHDTGSAVAAVPSEEEYPLYISSGTWSIVGTETDAPMI
                     NKESYDLNFANEGGANGRIRLLKNVMGLWIIQEIRRNLAIRGMQYTYGDLTRLAEEAE
                     PFAAMIDPDDEMFYEPSDMPQAVRDYCIRTGQRAPEDTGGIVRTVLEGLAFKYR"
BASE COUNT         1188 a          904 c         1216 g          962 t
ORIGIN      
        1 tggttaaata atcttattgg ttgtaataac aagtacaacg aaagaatact gcttggactt
       61 atttcatact gaaaatgtta atttttcata tcgacagccc atgttgatat gaaactgtaa
      121 atgcccgata atgtgaaatt tatttctgaa attgttgata taatgcccga agtctgttat
      181 aatgctcaat agcacaagca aaaatttcag cagccggctg aaaccgggag gcggcatcat
      241 atggtaatta cagtgacgat aaatccggca atggatattg tgatgaagtt ggatgatttc
      301 cgtctgaacg ttacgaacag gatacagaaa aagttcaggt gcgtaggcgg taagggtact
      361 catgtttcaa taaacctgag cctgctggga gtcaaaaaca cggcgacagg agtggtgatg
      421 gggtccacgg gcgaggagat aatcaggcgg ttgtctgagc acgatatcga tgtgaggttt
      481 ttaaagctca acaggggaga atccaggacc aactatgtca ttacggactc ggaaggcaat
      541 tgcacgctga tttccgaacc agggcagcaa tttgagaaag acgtggtcga aatgtttgtt
      601 tcccattatt cggagttggt cggcaaggac gatatggtgg tcatttcagg cgacgcatcg
      661 aaccagaagg ggacagggct ccaggacagg ctgataaaca tggccgttgc caggggagca
      721 cgtttctgcc tcgatgcaag cggcgaatat ctcgagaacg ggatcaggaa aaagccattc
      781 cttgtaaagc ccaacctgga cgagcttgga tcccttttcg gcagcgagat gaagactgaa
      841 gatgagatag ttgctgcaat caggcaggtc agtgcaaacg gcgccgagaa cattgtggcg
      901 tcctgcggcg gagaaggcag ttatgcgctg ataggcggca ggctgtaccg agccgttcca
      961 cctgcggtga aagcaaaaaa tactgtgggc tgtggtgatg cactgctatc tggtattatc
     1021 gccggattcg agatgaagct tcccgctgcg gatatcctga aaaaggctac agccgttgcg
     1081 gcggcgactg cgatggatga gtccaccgct ggcttcgatc cgtcggttgc agcaaaactt
     1141 gaacctgaag tgaaagtgga ggaattggaa ctgtagcagg gtaaacccga ctgcggcaac
     1201 gatcgcagcg gtttgaaagc gatgttatga tcgcggtgtt tcaaacacac cagacatgta
     1261 tcggaaaaca gaggaggagc attatggcta tccaatatta cgaggaaaga aaaaaactcg
     1321 ccggaacgat gaagctgctg tgggacagga agctcacaaa cgcggccggg ggcaatgccg
     1381 ccatgcgtgt cgctgaaaac aggataatca taactccgtc gatgatgtcg gaacacaggc
     1441 attgcgagat cgatgcggag gatatactgg tatgcgacta tgacctcaat gtaattgaag
     1501 gtacgggatt gctttcgaga gagtgcaaaa tgcatgcgct gcttctgaag aacataccgg
     1561 agatcggtgc ggtgatacat gcacatcctg aatatacgat ggtatacgca gccgcaaaga
     1621 aacctgttcc ttcggttaca gaggccaccc agaaaatggg cgactgcctg ttgatcaagc
     1681 aggcaaaggc gtattctgaa gatcttgctg aaaatgtgta tagatttttc agggataagc
     1741 gcgaacttct caggaagacc ggactttgcg gcatccttcc actgcacggc acggttgccg
     1801 tcggcaaaca ccttgaacag gctttcagtg tggtggaacg cgtggaatgc gatgcaaaat
     1861 gtaacatatt cggaaaaatg atctgaaagc ggtatggtga cactcctctg caagggggta
     1921 ttcctttatg gttgaggaat gtcacgcatc gaaagcggta nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn tggtgacact cctccgcaaa ggggtattcc tttatggttg
     2101 aggaatgtca cgcatcgaaa gcggtatggt gacactcctc cgcaaagggg tattccttta
     2161 tggttgagga atgtcacgca ttaatttata attaattcgg gttggttcat gacgttgata
     2221 taccggcagg acaaattcgt gcatggaggc tgtgccaggt gcttgcatat gagagaaaaa
     2281 gcaggataat agaatacctt gaacagaacg gcaaggcaga ggtcaatgtc ctggccaaac
     2341 tgctgggagt ggtcccggaa acgatccgca gggacctgcg ggagctcgaa acacagggcg
     2401 ttttgatgag gacccatggc ggagcggtga tcgaacatcc gaaagaaaaa gagtatcctg
     2461 tacaggtcag ggtcatgaaa aatgccctcg aaaaggatat gctttgccgc agggcagcag
     2521 ggttcataag cgaaggggac ctgatattcg ttgacaacag ttcgacgctt gtgaacctta
     2581 tcaagcatat aagcgatgac atacacctga cgatactgac caattccatc aaagtgctgc
     2641 aggaatatgc actgttcagg aaggacaata tcacgatgct ttgtacgggc ggggttttca
     2701 gcagatccaa cctgtctttg tccggtacgc tttccgacag ctttacgcgt gagttttttc
     2761 caaacaaggc gttcgtctca tgccatggag tatctgctga acatggcttt accgacggca
     2821 atctgcatga agtggcattc aggcgggaga taataagtct ggcggagaag gtttacttcc
     2881 tggtggacca ttccaagttc ggaaagctcg gaccgataaa actgggcggg cttgacattt
     2941 gtgatgtttt cataaccgat aaaaggccgc cgacggaact ggaacagcaa ttgaaacaga
     3001 gcaatccgga catggagatc atcgtatgca gtgaatgatt ctttattacg gcaggtatcc
     3061 ggccaatatc caacaggctt gcaacggata tgcaactgat acctgacaga tgacaggtat
     3121 ccaagagcat ttgcgtctat cgaagagtat atgcatgtat atatgaagca gccgattgca
     3181 gcaataaaaa tggacggaga tgtgacatga gacttgaaaa aaaggtgctt gctttcgatg
     3241 caggaggcgg aagcacgagg atttcgaaaa taggttttga cggaagcagg tttacgctgg
     3301 agcagatcgc caggttccca aacggacctg taagagcggg aggaaacctc tactgggaca
     3361 tccttggcat atggagggac ctgctggacg ggctcaggca ggcagtatgc gaggacagtg
     3421 aaatagcatc gctgggcatt gatacatggg gaaatgattt tgtacttctc gacaggaaag
     3481 gctgtatgct tgaaaacccg tatactcacc gtgacagccg taccaccggc cttgtcgggg
     3541 aagtcggccg gcttatcggc ggctgggacc tttacagcag gaacggcata caactgaacc
     3601 gtatgaatac attgtaccag ttgttttcca tatcccgaca gaggccttat atactggata
     3661 tcgccgacaa gttccttttc ctcgccgacc ttcttgccta ttaccttaca ggcgaaatga
     3721 aaaatgaata tacgatggcg acgatatcgc agctttacag ttacagtatg gcaggatggg
     3781 accatgagct catggaactg ctgggcatac ctgcacggat tttctgtcct gttatccggc
     3841 cgggagagat cattggcagt atttacggga aagtatgcag ggaagccggg atcccgccgc
     3901 tgaggctgac tgcggtcgct tctcatgata ccggctctgc cgtcgcggca gtgccatccg
     3961 aggaagaata cccgctgtac ataagcagcg gaacctggtc aatcgtcggc acggaaaccg
     4021 acgctccaat gataaataag gaatcgtatg atttaaattt tgccaatgag ggcggagcaa
     4081 acggcaggat caggctgctc aaaaacgtga tggggctgtg gataatacag gagataaggc
     4141 gcaatctggc tatcaggggg atgcagtaca catacggcga tctgacaagg cttgcggaag
     4201 aagccgagcc gtttgcagcc atgatagatc ctgatgacga aatgttttac gagccgtcgg
     4261 acatgccgca ggcagtcagg gattactgca tcaggacagg gcagagggcg ccggaggata
     4321 cgggcggtat cgtgaggaca gtgctggaag gcctcgcatt caaatacagg
//
LOCUS       DAOSCC010000202         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12701_length_4349_cov_4.992548, whole genome shotgun sequence.
ACCESSION   DAOSCC010000202 DAOSCC010000000
VERSION     DAOSCC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12701_length_4349_cov_4.992548"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLG72_09620"
     CDS             <1..771
                     /locus_tag="PLG72_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81093.1"
                     /translation="VEIIRKAREKGLVVETNWDWHKDEVRQVARMLGIDESIASRQPF
                     PGPGLAVRVVCNDGSSKVDEEDIKKFNRLLVDSKTAHSGQILPVKTVGVQGDCRSYRH
                     LSVLWGQGTEFDWDEVYELSKVITNNVHSVNRAAYILNKTSIYGGIKTYEMYINKESV
                     NLLREIDHIVTSSLNAKTISQSFAVLLPIGIEKRYSVAIRTFVTNDFMTGRPAFIGKD
                     ESRDVIRELAKKIGSTFSEIEFVLYDVTSKPPATCEWQ"
     gene            808..972
                     /locus_tag="PLG72_09625"
     CDS             808..972
                     /locus_tag="PLG72_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81094.1"
                     /translation="MFEIRGKYNTAKVFTDNIDSSAIAQFNTIELSRYEADRGSRIKA
                     AGIKRFRLFQ"
     gene            1027..1596
                     /locus_tag="PLG72_09630"
     CDS             1027..1596
                     /locus_tag="PLG72_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81095.1"
                     /translation="MINAVNSTNRLQPAYTNEKGSPGRQDNIKKDETGAILELGRSEA
                     RSAVYGKPVYGRDTKEIDRLWREAEKSYESLREIVVRLVVRQGKKYEDVLSGKEALSV
                     DESARAEARMLLSEDGELGVKAVSARIVNFAKALSGGDRSKLAELKAGIEKGFREAER
                     VFGGKLPQICYDTYDEIMRQLDEWANEDN"
     gene            complement(1744..1998)
                     /locus_tag="PLG72_09635"
     CDS             complement(1744..1998)
                     /locus_tag="PLG72_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81096.1"
                     /translation="MSCYYIIGVRMDNRIDNAVKFQEVLTKNGCRIKARLGLHEVSDD
                     ACSNDGIIILQPYGSKEDVEALVKELNSLEGVTAKYIDLN"
     gene            2281..3255
                     /locus_tag="PLG72_09640"
     CDS             2281..3255
                     /locus_tag="PLG72_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81097.1"
                     /translation="MIDLNIIVDKLLDMKPDPIPEFILLKEFRKISPDSREYQDAYDK
                     VCSHPFVKGIESEQNERGFWDPFHGYTEEVIRRCLSLGLDRDHPCFRRVAEYLVKVLS
                     NEESWDQSEKQDNVRWWPEMFVPLVSAAMLSLIDPDNEILSIHRRRWANFAGIAFAKG
                     QYDKEAESKAQHEYFGFVTKRTIPAYGYYNLLLLAPTDKASFISDAVDGALVDHCMNC
                     IDEIYYVYNCGLSDFVPIGTGRRDSRDFCHWVRALALVSQFRGWKKYKERYDEWILSQ
                     RNEEGLWELPKKPRRYDFPLSDSWRTRKNRVIDSTIMVLRFLNNSKAF"
     gene            complement(3441..3638)
                     /locus_tag="PLG72_09645"
     CDS             complement(3441..3638)
                     /locus_tag="PLG72_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81098.1"
                     /translation="MENILKVVETGIRKNEIRPLDARSITSAFYNMMIGLAMNVRFFT
                     GEQLDEEISRCPDAFLAGIKA"
     gene            3858..>4349
                     /locus_tag="PLG72_09650"
     CDS             3858..>4349
                     /locus_tag="PLG72_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HOJ81099.1"
                     /translation="MLKKAKVTVHPDYRIGKVEKRLFGAFLESIGNTVYGGIYSPKHP
                     TADDLGFRRDIIDAVKEFDLPAVRLPGGNWVSGWDWKGSIGPRENRRAQLDLAWFKIE
                     PNIIGHDEYIEWTMRANTEPMYTINLGTGDINSAAHLVEYSRYEGGTYWSDLRRKYGH
                     PEPY"
BASE COUNT         1255 a          948 c         1162 g          984 t
ORIGIN      
        1 gtcgagatca ttcgaaaggc aagggagaaa ggcctggtag tggaaacgaa ctgggactgg
       61 cacaaggatg aagtaaggca ggtagccagg atgctgggca tcgatgaatc gatcgcttca
      121 aggcagccat tccccggtcc cgggcttgct gtcagggtag tttgcaatga cgggagcagc
      181 aaagtagatg aagaagatat aaagaaattc aacagactgc tggtcgattc gaagactgcg
      241 cacagtgggc agatattgcc tgtaaaaacc gtcggcgtcc agggggactg caggagttac
      301 aggcatttgt cggtactgtg ggggcaaggg accgaatttg actgggatga ggtatacgaa
      361 ctttcgaaag tcattacgaa taacgtacat tcagtgaaca gagcggcgta tatactcaat
      421 aaaacaagca tatacggcgg cattaagact tacgagatgt atataaacaa ggaaagtgta
      481 aacctgctga gggagataga ccacatcgtc acatcttctc ttaatgcgaa aaccataagc
      541 cagtcattcg ccgtattatt gcccataggg atagaaaaac gctattctgt agctataaga
      601 acattcgtca cgaatgattt catgactggc aggcctgcgt ttatcgggaa ggacgagagc
      661 agggacgtga tacgcgaact ggcaaagaag atcggatcca ccttcagcga gatcgagttc
      721 gtcctgtacg atgtgacaag caagccgccg gcgacctgcg agtggcagta agcagagacc
      781 gcatagtatg gctgcggatt aagcaaaatg ttcgagatca gaggcaaata caatactgca
      841 aaggttttta cagacaatat cgacagttcg gcgattgcgc agttcaacac tatcgagtta
      901 tcccgttatg aagcagatcg cggcagccgg atcaaggctg ccggcataaa aaggttcagg
      961 cttttccaat aatatgccga tatatattac atatggatgt attttggttc cagggaggga
     1021 aacaatatga tcaatgctgt caattccacg aacaggctcc agcctgccta cacaaatgag
     1081 aagggatcac caggaagaca ggataacata aaaaaggatg aaacaggggc aatcctcgaa
     1141 ctggggagga gtgaagcccg gagtgccgta tacggcaaac cggtgtacgg aagagacacg
     1201 aaggagatag accgcctttg gagggaagcc gaaaaatcct atgaatctct cagggaaata
     1261 gtcgtcaggc tcgtcgtccg gcagggcaaa aagtatgaag acgtgttgtc gggaaaggaa
     1321 gcgctttctg ttgacgagag tgccagggct gaagcccgga tgctgctgtc cgaggacggc
     1381 gaactgggag taaaggctgt cagcgccagg atcgtgaact ttgccaaggc actttcaggc
     1441 ggcgacagat ccaagctggc tgagcttaag gccggtatag aaaaaggctt ccgtgaggcc
     1501 gaaagggttt tcggcggcaa gctcccgcag atatgctacg atacctatga tgagatcatg
     1561 agacagcttg atgaatgggc gaatgaagat aattgaacgg ggtcagggta gaccccgttt
     1621 tattttccgt gccccgtgac gcaataaacc gcataattaa attactcgga cagacattat
     1681 aaaacagaca actgtataac aaccggccga agaggtttat gctttttctg cattaaaatt
     1741 atatcagtta agatcgatat actttgccgt tacgccttca aggctgttga gttctttaac
     1801 aagagcttca acgtcttcct tgctgccgta cggctgcaat atgatgatcc cgtcattgga
     1861 gcaggcatcg tcgctcacct catgcaatcc caggcgcgcc ttgatcctgc agccgttttt
     1921 cgtgagcact tcctggaact ttaccgcatt gtcgatcctg ttgtccatcc ggacgcctat
     1981 aatatagtaa caagacatca ggcaaccctc ctgtgcgcca ataggtccga ttaaatcaat
     2041 tacattatat cattccgaac tcctgataca aaccatgatt tatcataagc tttttcgatg
     2101 catattaaga aaccgcatta aaagcaaact gatccagcta aagcaaatgt ttccgaataa
     2161 aaggataaaa ggcaaatgtg tcgaattcct atagaaattg caagtgcaga taatgttgac
     2221 caaccggcag gatccaaatg aaaacaggaa accatcgcca tatcgtaaag gaggtcactg
     2281 atgatcgatc ttaatattat cgtcgacaaa ctcttggata tgaaacctga cccgatccct
     2341 gagtttattc ttctgaagga gttcagaaaa atcagccctg acagccgtga ataccaggac
     2401 gcgtatgaca aggtgtgcag ccaccccttt gttaagggca tagagagtga gcagaatgaa
     2461 agagggttct gggatccatt tcacggctat acagaggaag ttatccgcag gtgcctttca
     2521 cttgggctgg acagggatca tccctgcttc agaagagtgg ctgagtacct ggtcaaggtg
     2581 ctgagtaatg aggaaagctg ggaccagtct gaaaaacagg acaacgtccg ttggtggccg
     2641 gagatgtttg tcccgcttgt cagcgccgcc atgctttctc ttatagatcc ggacaacgag
     2701 atacttagca ttcacaggcg ccgctgggca aatttcgccg ggatcgcctt cgcaaagggc
     2761 caatatgata aagaagcgga aagcaaggcg cagcacgagt attttggatt tgtcacgaaa
     2821 cgtaccatac cggcgtacgg gtactataac ctgcttttgc tggcgccgac ggataaagcg
     2881 agcttcatca gcgatgccgt tgacggggct ttagtggatc actgcatgaa ctgtatagat
     2941 gagatctatt atgtttacaa ctgcgggctg tcggatttcg tcccgatcgg aaccggaaga
     3001 agggacagca gggacttttg ccactgggtg cgtgctttgg cgctggtctc gcagttcagg
     3061 ggatggaaga aatacaagga aagatatgat gagtggattt tgagccagag aaatgaagaa
     3121 gggttatggg aactgccgaa aaaaccgaga cgttatgact tcccgctgtc agattcatgg
     3181 aggaccagga aaaaccgcgt tatcgacagt accatcatgg tcctgcggtt cttgaacaac
     3241 agcaaggctt tttagcggat tttatacaag cgggaaaaga gtaaacagga tctcaggcca
     3301 tttgaaatgc ttttggaacc aggcttgaaa catctgctat tcggtcagca ataaggaggt
     3361 tgttcaggcc tggctccgaa actcaactaa gatcagctta gggactgtat taagatcaaa
     3421 attatgaaca aaccggatta tcatgcctta atgcctgcca aaaacgcatc aggacagcgg
     3481 ctgatttctt catcgagttg ttcgccggtg aagaatctga cattcatcgc aaggccgatc
     3541 atcatattgt agaaggctga tgttatgctg cgcgcgtcca atggccttat ttcgtttttc
     3601 ctgatgcctg tttcgaccac tttgagtata ttttccataa aatccatccc gacctgggaa
     3661 gttttgctct gaagctcctt tgcaaagcaa tcgggcgggc agtagtaata ccggtccgcc
     3721 tgcaaggggt aaatgcggcg gatgcaaaat acggccaatt cggtaaaatt tcaaaaaggc
     3781 tctattgttt tgtgcagaca taagatatga ttatattatg aaaaggaaaa acgatttacg
     3841 aacgaggagg gcaggatatg ttgaagaaag ccaaagtcac agtacatccc gattacagga
     3901 taggcaaggt cgagaagcgg ctgttcggcg catttctcga atccataggc aacacggtat
     3961 acggcggcat atacagcccg aagcacccga ctgcggacga cctgggcttc cggcgggata
     4021 tcatcgacgc ggtgaaggag ttcgacctgc ctgctgtccg gctgcccggc ggcaactggg
     4081 tttccggctg ggactggaag gggtcgatag gaccgcgtga aaacaggagg gcgcagcttg
     4141 accttgcatg gttcaagatc gagccgaata tcatcggcca tgacgaatac atcgaatgga
     4201 cgatgcgcgc caatacggaa ccgatgtata ccataaacct cggcacggga gatataaaca
     4261 gcgcggctca cctggtcgag tattcaaggt acgaaggcgg tacatactgg tctgacctga
     4321 gaaggaaata cgggcatcct gaaccgtat
//
LOCUS       DAOSCC010000203         4315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12841_length_4315_cov_3.472300, whole genome shotgun sequence.
ACCESSION   DAOSCC010000203 DAOSCC010000000
VERSION     DAOSCC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12841_length_4315_cov_3.472300"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLG72_09655"
     CDS             <1..514
                     /locus_tag="PLG72_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOJ81100.1"
                     /translation="TGYSTADFNLWPSFSKAILVLLMFIGASAGSTAGGMKCIRIVLL
                     MKLVRREIHRIIHPRSVYTVKIGGKTVNDDVLSGVTVFFYTFILIFAVALLIVSVEGK
                     DMVSNFTAVAACIGNIGPGLGIVGPMGNFSAYSAISKAVFSFCMFAGRLEIFPVLLLF
                     APAFWKKVNI"
     gene            complement(640..1389)
                     /locus_tag="PLG72_09660"
     CDS             complement(640..1389)
                     /locus_tag="PLG72_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ81101.1"
                     /translation="MMNSFTRNYTDNSTEAGFQFTFYCDLCNDGFKTSFIESETYKKG
                     SLFRGISGGARALGHLLGGSLGHMAYSAGYGSDVLSERFRGMSPEWQKEHEKAFIQAQ
                     NEAKQHFNRCHGCRRWVCDADFNEDEGMCVECAPRQNITIAKAKANRMVQQIEEAAEN
                     ANVFRGAIESKTTVCPNCGKPAGGGKFCSNCGYNLQLRQCPNCGAQAAQGVKFCSECG
                     AKLASPSACSNCGTVNPPNTRFCGECGNKLM"
     gene            complement(1436..1765)
                     /locus_tag="PLG72_09665"
     CDS             complement(1436..1765)
                     /locus_tag="PLG72_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81102.1"
                     /translation="MAFYKEPCIHCGYLLPRDSQFCPGCGSRSPFIYSCPTCLREIRK
                     EESYCSGCGRPLYIICPKCMEKTFVQESCEHCGESLMIQCPNRRCQAMQFFQNKKCTA
                     CGKVLSK"
     gene            complement(1992..2552)
                     /locus_tag="PLG72_09670"
     CDS             complement(1992..2552)
                     /locus_tag="PLG72_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ81103.1"
                     /translation="MTLLRLFWSFFLVGLFSFGGGYAALPLIQEQVIEINSWLTPDEF
                     ADILTISQMTPGPISINASSFVGTKIAGIPGAIAATAGCVTPSCIIVLILAALYYRFK
                     GLSMVQGIIRGLRPAVVALIASSGISILLTALFGTAEFPISLSGLDPAALGLFIASLF
                     MLRRFKPDPIFVMLGAGIIGLIVYSI"
     gene            complement(2549..3148)
                     /locus_tag="PLG72_09675"
     CDS             complement(2549..3148)
                     /locus_tag="PLG72_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOJ81104.1"
                     /translation="MKKDARFYWNLFFSTLKLSAFTFGGGYVIVPLMQKRFVKELKWI
                     DEDEMLDLVAIGQSAPGPVAVNASIMVGYRMAGVPGAFLTVLGTVLPPLVIITAITFF
                     YMAFRDNQVIRTLFRGMQAGIAAVIADVVINMAAKIIKDRRVLPITVMALSFLAASFF
                     DVSIIVILLVSGLLGVYTAFRTKGGAETDPRRTEEEGFE"
     gene            complement(3256..3447)
                     /locus_tag="PLG72_09680"
     CDS             complement(3256..3447)
                     /locus_tag="PLG72_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOJ81105.1"
                     /translation="MKASIDRDGCIACELCATTCPEVFRMADDGLAEVYVDEVPEGSE
                     ECAKEAEEGCPVSVIRVEE"
     gene            3686..>4315
                     /locus_tag="PLG72_09685"
     CDS             3686..>4315
                     /locus_tag="PLG72_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOJ81106.1"
                     /translation="MKKISKTVLAILILIIFAVLAARKILPEAGIDTLFCAGGEKRPD
                     MSEDVTYMILKPGKTQGRQVTGADGSEAGKSGNGEGNHIDEDGRSGAGTEYLINEEGT
                     TVAERIKPPEGFERVELDAGSFGEFLRNLPLKPHGTKVRYYDGRVKPWDVHAAVIDMD
                     VGEKDLQQCADAVIRLRAEYLFSKGLYDQIHFNFTNGFNAEYKKWMEGYR"
BASE COUNT         1005 a         1091 c         1132 g         1087 t
ORIGIN      
        1 gacaggctat tcgaccgctg atttcaacct gtggccgtcc ttcagcaaag ccatactcgt
       61 gctgcttatg ttcataggcg ccagcgccgg atctacagcg ggcggtatga aatgcatccg
      121 tatcgtattg ctgatgaagc ttgtgcggcg tgaaatacac aggatcatcc atccccgttc
      181 ggtctatacc gttaagatcg gcggtaaaac agtcaacgat gacgtgctgt ccggtgtaac
      241 agttttcttc tacactttta tattgatatt tgcggtagct ctccttatcg tatctgtcga
      301 aggcaaagac atggtatcga acttcactgc cgttgctgcc tgcataggca atattggacc
      361 cggcctcggg atcgtcgggc cgatggggaa tttctcggcg tattcggcga tcagcaaggc
      421 agtcttctca ttctgtatgt tcgcgggcag gctggagata ttcccggtac tgctgctctt
      481 tgctccggcg ttctggaaaa aagtcaatat atgatacaaa acatcataca gacaggggga
      541 ctgtttccat gtctctatta tgagacggac cgtccccctg tcctgaaatt tccatccatc
      601 atgtatatat aaagtgtcca ggcgctgtga caggcatctt cacatcagct tgttcccgca
      661 ttcgccgcag aagcgcgtgt tgggcgggtt taccgtgccg cagttgctgc aggcggaagg
      721 ggaagcaagc tttgcgccgc attcactgca gaacttgacg ccttgcgccg cctgtgcgcc
      781 gcagttgggg cattgtctga gctgcaggtt gtatccgcag ttgctgcaga attttccgcc
      841 tccagcaggc tttccgcagt tggggcatac ggtggtcttg ctttcgatgg cgcccctgaa
      901 tacgttggcg ttttcggcag cttcctcgat ctgctgcacc atcctgttcg ccttggcctt
      961 tgcaatggtt atgttctgac gaggtgcgca ttcgacgcac atgccctcgt cttcgttgaa
     1021 gtccgcgtcg catacccacc tgcggcatcc gtggcacctg ttgaaatgct gttttgcttc
     1081 attctgggcc tggataaagg ctttttcatg ttctttctgc cattcggggc tcatacccct
     1141 gaacctctcg gaaagtacgt cgcttccata gcctgcgcta tatgccatat ggccgagcga
     1201 tccgcccaga aggtggccaa gcgcccttgc tcctcccgat atgccgcgga acagcgaacc
     1261 ctttttgtaa gtctccgatt cgatgaatga cgtcttgaag ccgtcattgc acagatcaca
     1321 gtagaatgtg aactggaaac ctgcttctgt gctgttgtcg gtataattgc gcgtaaaaga
     1381 attcatcatg gctggaccct ccgatcagtt atatttacat aaatattgaa ggcgcttatt
     1441 tgctgagcac ttttccgcat gccgtgcact tcttgttctg gaagaactgc attgcctggc
     1501 agcgcctgtt ggggcactgg atcattaaac tttcaccgca atgttcgcag ctttcctgta
     1561 cgaacgtttt ttccatgcat ttggggcaga ttatgtaaag gggtctgccg catcctgaac
     1621 agtaggattc ttcctttctg atctcgcgaa ggcatgtggg acaactgtaa atgaaagggc
     1681 ttctgctgcc gcagcccgga cagaactgtg agtcccgtgg caaaaggtat ccgcagtgga
     1741 tacacggttc tttatagaaa gccataacaa cacccgcctt tcaaatatca ttgtaggtaa
     1801 agcgctgcct gatgtaatca tctttcaggt atgaaatgtg taaaacacag gggtgatttt
     1861 gggggtgata tcttgataaa ggcgacggct gatacatggg aaatgttgat acatgcggac
     1921 ggtccgcgtg tccggtttcc gcggaatcgg gatacaggaa ccatattcca gcctgtgtta
     1981 taccaggtat tctatattga ataaactatt aagccgataa tgcctgctcc cagcatcacg
     2041 aatatgggat caggtttgaa cctcctcagc atgaacaggc tggcgatgaa cagtccgagt
     2101 gctgccggat ccaggccgga caggctgatt gggaattcgg ctgtgccgaa taacgccgtc
     2161 agaaggatgg atatgccgga ggaagctatc aaagctacta ctgcgggacg aagccctctt
     2221 atgatcccct ggaccatgct gaggcctttg aacctgtaat aaagcgcagc caatatgagc
     2281 actatgatgc aggaaggcgt cacgcatccg gccgttgccg cgatcgcgcc cgggatcccc
     2341 gctatttttg tgcctacaaa ggaggatgcg ttgatcgata tcggccccgg agtcatctgg
     2401 gatatggtca ggatatcagc gaactcgtcg ggagtcagcc aggagttgat ttctatgacc
     2461 tgttcctgta tcagcggcaa agccgcatat cctcctccga agctgaacag gccgacaagg
     2521 aagaaactcc agaacagcct gagtagtgtc attcgaatcc ctcctcctca gtgcggcgcg
     2581 gatccgtttc tgctcctcct ttggtccgga aggccgtata tactccaagc agtccggaaa
     2641 caagaaggat gacgataata ctgacatcga agaatgaagc agcgaggaaa gaaagcgcca
     2701 ttacggttat gggcaggacc cgtctgtcct ttattatctt tgctgccatg tttatgacca
     2761 cgtcggctat gactgcggca atacctgcct gcatgcccct gaacagggtc cgtattacct
     2821 ggttgtcgcg aaatgccatg taaaagaatg ttattgccgt aatgatcaca agcggcggca
     2881 atacggtgcc gaggaccgtg aggaacgcgc ccgggacgcc ggccatcctg tatcccacca
     2941 tgatcgatgc gttgacagcg accggtccgg gcgccgactg acctatggca accaggtcga
     3001 gcatttcatc ctcatctatc catttcagtt cctttacaaa gcgcttctgc ataaggggaa
     3061 cgatgacata accgcctccg aatgtgaaag cgctcagttt taatgtggaa aagaacaggt
     3121 tccaataaaa gcgtgcatcc tttttcaacg gtcttctcct ttagccggca aaatcatcca
     3181 ttgaccggca aacctaagta tcgcatgctt ttatcagtat accccggatc ataagatttt
     3241 gccggttgtt tagaattatt cctcgaccct tatgaccgag acagggcatc cctcctcggc
     3301 ttcttttgca cattcctcgg aaccttcggg gacttcgtcc acgtaaacct cggcgaggcc
     3361 gtcatccgcc atgcggaata cctccgggca ggtagtcgca cacagttcgc atgcgatgca
     3421 gccgtctctg tcgatagatg ctttcattgc ttattactcc tttactgttt atatttcata
     3481 caaaccgtac ataatattat gccgcaaata ccaggcatat atttcgtgtt tggtttgcat
     3541 tctgcactat ttattgtttc caacagcttt atgcgttgaa acaattttcg gatttttccg
     3601 tctatgcctt acagggacat gccataccgg caggtgccga aaaaagctcg ggatgatacc
     3661 cgggatgggc agggagaaag gtgacatgaa gaagatttcc aaaactgtac tggctatcct
     3721 gatcctgatc atattcgcag ttctggccgc caggaagata ctgcctgagg ccggcattga
     3781 tacgctgttt tgcgcaggcg gtgagaaaag accggatatg tccgaagacg tgacttacat
     3841 gatactgaaa cctggcaaga cgcaagggcg gcaggtgacc ggtgcggacg gcagtgaggc
     3901 gggaaaatcc ggcaatggtg aaggaaatca catcgatgag gacgggaggt ccggcgccgg
     3961 tacagagtac cttatcaatg aggagggaac caccgtcgcc gagaggataa agccgccgga
     4021 aggcttcgag cgcgtggaac ttgatgcagg ctcgttcggc gaattcctgc ggaacctgcc
     4081 tctcaagccc catggcacca aagtcagata ttatgacggc agagtgaagc catgggacgt
     4141 tcatgcggca gtgatcgata tggacgtggg ggagaaggat ctgcagcagt gtgcggatgc
     4201 cgtgattagg ctccgtgcgg agtatctttt ctcaaaaggg ctgtacgacc agatacattt
     4261 caatttcacc aacggcttca acgccgagta taaaaagtgg atggaaggat acagg
//
LOCUS       DAOSCC010000204         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12858_length_4310_cov_4.434078, whole genome shotgun sequence.
ACCESSION   DAOSCC010000204 DAOSCC010000000
VERSION     DAOSCC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12858_length_4310_cov_4.434078"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLG72_09690"
     CDS             <1..517
                     /locus_tag="PLG72_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ81107.1"
                     /translation="GSTLVVPVSASHAIEKLAEEYQGSVVRTRTAARDIMAGLLARET
                     KEELMEQFTMHFDAAAGLVKLLDFMKTNGTSLHEMVDMLPKIHMRRTEVECSWDAKGR
                     VIKKIIQEHYGSKVEMIDGVKVYNDKGWVLVLPDADRPVCSVIGEGFSEEFAEELTNI
                     YARKIREISRG"
     gene            654..1379
                     /locus_tag="PLG72_09695"
     CDS             654..1379
                     /locus_tag="PLG72_09695"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ81108.1"
                     /translation="MNKPAGRYILFIVFMLLGIVMAWQLKSTLNAKRVAASSAFNANA
                     LREEITELKQDIEELRAAIDENIALQNDIIKEYIALQNDNQLSSEWEIIKLHTGLVGV
                     EGPGITVTLDDAPVREPDMPPSWFIIHDSDMRVILNDLKKAGAQAIAINGERVVPMSE
                     PVCAGPTIIINGNRYPVPFIIEAIGDPDVLIEAMNLSSKVAELKQFNIQVDVKKSEKI
                     TISKFSGADKLDRYISGLEVVEK"
     gene            1376..2074
                     /locus_tag="PLG72_09700"
     CDS             1376..2074
                     /locus_tag="PLG72_09700"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ81109.1"
                     /translation="MKITRNLAIMMVCMLLGIMLALQYRSVNYNQSMASLENKRLEEL
                     KEEVIRLQNQKNELQERYQKLEEENQTFARVKAGDSEAVQQLKNSLLKARIFAGLETV
                     KGAGLIVTLDDSYPYFYVSDFDILNVVNELRASGAQAISVNEERIVAMSEIRDAGDYI
                     VINGKQFKAPFVIKAIANPNNLERALMMIGGVLENLEKDGIKVSVKKSDELVIYKFVD
                     DGTSIRTDLLTPVE"
     gene            2279..3283
                     /locus_tag="PLG72_09705"
     CDS             2279..3283
                     /locus_tag="PLG72_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ81110.1"
                     /translation="MILCFSGCSEPSAYNPALSDLGGVYASSFDDLDIEIEISGRLSE
                     EDAEESGTYAKENWGTGDDYSDDPEYDEDPERTVFEGEVGFAGLADGGESNKLLRWGI
                     SRRPDNLPPKADPGAPELLTKYGGAWIGDTEKSVIYLTFDEGYENGYTPMILDTLAKH
                     NVKAVFFITGPYLKEHQDLVRRMVEEGHIVGNHTIHHPSLPTLDDRQVEEEILGLERA
                     FREKFGRGMEFLRPPKGEYSERTLRIAKDLGYCSLFWSFAYDDWYRDKVRGPQYAYDI
                     VMRNLHNGAVLLLHAVSKDNAHALDSIIKGAAERGYSIGDPRDLLPSGKAGDATYGEK
                     "
     gene            3270..3611
                     /gene="yqfC"
                     /locus_tag="PLG72_09710"
     CDS             3270..3611
                     /gene="yqfC"
                     /locus_tag="PLG72_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HOJ81111.1"
                     /translation="MGKSNAADTKETKKNAGSGNSTVRKSAREKFTEMLELPKDLVLD
                     RPKLTLIGNRDLMVENYKSVLEYDAGILRINTGTGIIRITGSDLSIREITSEDIVVSG
                     MVRSVEFVDAG"
     gene            3617..>4310
                     /locus_tag="PLG72_09715"
     CDS             3617..>4310
                     /locus_tag="PLG72_09715"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOJ81112.1"
                     /translation="MLFRLWNYLRGYVIIIVEGFFIEKFINICARRRIHLWDVRIQRE
                     RRVTARVSISGFRLIRPVARKTGCRVRLLKKRGLPFVFSKYRRRKAFFAGAVLFVVLI
                     NVLASFVWSIEITGNEKLDTVFLENVLAANGIKTGALKYRIDTRRAVDRMMLEIDRLS
                     WISINIKGTKVKVQVREREDIPEIVPRHIPCDIIARKDGIIRQVIAKEGIEAVAEGDT
                     VKKGQVLISGRVP"
BASE COUNT         1286 a          869 c         1228 g          927 t
ORIGIN      
        1 agggagcaca ctggtcgtgc ccgtttcggc aagccatgca atagaaaagc ttgccgaaga
       61 ataccaggga agtgtcgtac gtaccaggac ggcagcaagg gatatcatgg caggactgct
      121 ggcaagggag accaaggagg aactgatgga acagttcacc atgcattttg acgctgcagc
      181 tggcctcgtg aaactccttg attttatgaa aaccaatgga accagcctgc atgaaatggt
      241 agacatgctg ccgaagatcc atatgcgcag gacggaagtg gaatgcagct gggacgcgaa
      301 aggaagggtc ataaaaaaga taatacagga gcattacgga agcaaggtgg agatgatcga
      361 cggggtcaag gtatataatg acaaaggctg ggtgctggtc ctgccagatg cggacagacc
      421 tgtctgcagt gtaataggag aaggcttcag cgaggaattt gcagaagaac tgaccaacat
      481 ttatgcaaga aaaatcaggg aaataagccg tggttaggaa ataatcaata ttttgaattt
      541 attacggctt cagggataaa tgtaagattc gatcatggaa cctttcactt gaaatcggtg
      601 tgcttaacaa gtagaattga gttaagacac cgaatttctt tgtaggagtt gttatgaaca
      661 aacctgctgg ccgttatatt ttatttatag tgtttatgct gcttggaata gtcatggcat
      721 ggcagctgaa aagtactctc aatgcaaaga gggtcgcagc ttccagcgct ttcaacgcca
      781 atgccctcag ggaagagata acggaactga agcaggatat cgaggaactc agagctgcga
      841 tcgatgaaaa catagcattg cagaatgata tcataaaaga atatattgca cttcagaacg
      901 acaatcaact gagcagtgaa tgggaaatca tcaaactgca taccggcctt gtgggagtag
      961 agggacctgg gatcacggta actctcgatg acgcaccggt acgtgagccc gatatgcctc
     1021 cgtcatggtt catcatacat gacagtgata tgagggtcat actgaacgac ctgaaaaaag
     1081 ccggagcaca agccatcgca ataaacggcg aacgtgtggt gccgatgagc gagcccgtat
     1141 gtgcaggtcc gacaattatc atcaacggaa acaggtatcc tgttccgttt atcatagagg
     1201 cgatcggaga tcctgatgtg ttgatcgaag ctatgaacct gagcagtaaa gtggcggaac
     1261 tgaagcagtt caatatccag gtcgacgtaa aaaagtcgga aaaaataaca atatccaaat
     1321 tcagcggtgc tgataaactg gacagatata tatcgggact ggaggtagtc gaaaaatgaa
     1381 aatcacgcgc aatctggcga taatgatggt gtgtatgctg ctcggcataa tgctggcgct
     1441 tcagtacaga agcgtgaatt ataaccagag catggcaagc cttgagaata aaagactcga
     1501 agaactgaag gaagaagtga taaggcttca gaaccagaag aacgagctcc aggaaagata
     1561 tcagaagctg gaggaagaaa accagacttt tgcaagggtc aaggcaggag acagcgaagc
     1621 cgttcagcaa ctgaagaaca gcctgctgaa ggcaaggata tttgccgggc ttgagaccgt
     1681 caagggagca gggctgatag ttacgctgga cgacagctac ccctactttt atgtatctga
     1741 tttcgacata ctcaatgtgg taaatgaact cagagccagc ggcgcgcagg caatatccgt
     1801 caatgaagag aggatagtcg ccatgtcgga gatacgtgat gcaggggatt acatcgttat
     1861 caacggaaaa cagttcaagg ctccgttcgt gataaaagcg atagccaacc ccaataacct
     1921 ggaacgtgca ttgatgatga tcggcggagt ccttgagaac cttgagaaag acggtattaa
     1981 agtaagcgtc aaaaaatcgg atgaactggt gatctataaa ttcgtcgatg acggcacgtc
     2041 tatcaggaca gatctgctga cacccgtgga ataaaggatg ctgtaaagta atcggcatat
     2101 tttcctgcct ccataataaa attaaagaag caggcaatat ggccttgact tggataccat
     2161 acgattttgg catgcaagcc gggctttttt gcttattttt tccggaggtc gtacaatggg
     2221 agctttcagg aactttaatt ttcgctgcac cgggacggcg gcagtggcag cattctgcat
     2281 gatcttatgt ttttcaggct gttctgaacc aagcgcatac aacccggctc tgagtgatct
     2341 tgggggtgtt tatgccagca gtttcgacga cctggacatt gagatcgaga taagcggaag
     2401 gctgtcagag gaagatgcgg aagaaagtgg cacatacgcg aaggaaaact ggggcaccgg
     2461 cgatgattac agtgatgacc cggaatatga tgaagacccg gaacgtacag tattcgaagg
     2521 ggaagtcgga tttgcaggcc ttgctgacgg aggggaaagc aataaactcc tgagatgggg
     2581 catatcgaga aggccggaca atctgccgcc gaaagctgat ccgggcgctc cggaactgct
     2641 aacgaagtat ggaggggcat ggataggcga cacggaaaaa agcgtcatat acctgacctt
     2701 tgatgaagga tatgaaaacg gatatactcc aatgatactt gataccctgg caaaacataa
     2761 tgtcaaggct gtttttttca taacaggacc ttatctgaaa gagcaccagg accttgtaag
     2821 gaggatggtg gaggaagggc atattgtcgg gaatcacacc atacaccatc caagcctgcc
     2881 gaccctggat gacaggcagg tggaagagga gatcctcggg cttgaaagag cgttcaggga
     2941 aaagttcggc aggggcatgg agttccttcg tccgccgaaa ggagagtata gcgagaggac
     3001 gctccggatc gccaaagatc ttggttactg cagcctgttc tggagtttcg cgtatgacga
     3061 ctggtaccgt gacaaggtaa gagggccgca gtacgcctac gacatcgtca tgcggaatct
     3121 gcacaatggc gccgttttac tgctgcacgc cgtatcaaag gataatgccc atgctcttga
     3181 cagcatcata aaaggtgcgg cggagagggg atacagcatt ggcgatccac gcgatctgct
     3241 gccgtccggg aaggctggag atgcgacata tggggaaaag taacgccgcc gatacgaagg
     3301 agaccaaaaa aaatgccgga agcggaaaca gcaccgtaag gaagagcgca agggaaaagt
     3361 tcacggaaat gcttgagctc cccaaagatt tggtattgga ccgcccaaag ctgaccctga
     3421 tcggcaacag ggatctgatg gtcgaaaact ataaatccgt cctggaatat gatgccggca
     3481 tattgaggat aaatacgggg accgggataa tcaggataac cggatccgat ctgtcgataa
     3541 gggaaatcac ttcggaagat atcgttgttt caggaatggt ccgctcagtc gaattcgtcg
     3601 atgcggggtg aaattcttgc tgttcagatt gtggaattat ctgcgcggat atgttattat
     3661 tatagtcgaa ggctttttca tagaaaagtt cataaacatc tgcgccagac gccggataca
     3721 tctgtgggat gtcaggatcc agagagagcg ccgggtgacg gcgagggtca gcatcagcgg
     3781 gttcaggctc atcaggcctg tcgcaaggaa gacaggatgc agggtgaggc tgctgaagaa
     3841 aaggggcctg ccgtttgtgt tcagcaaata tcgcagacgg aaggcatttt ttgcaggagc
     3901 cgttttgttc gttgtgctga taaatgttct tgcgtctttc gtatggagta tcgagatcac
     3961 cggtaatgaa aagctcgata ccgttttcct ggaaaatgtg cttgcggcca acgggataaa
     4021 gacaggcgcg ctgaaataca ggatcgatac ccgccgtgct gttgacagga tgatgctgga
     4081 gatcgacagg ctatcctgga tcagtataaa tataaaggga acaaaagtaa aggtgcaggt
     4141 gagggaacga gaggacatcc cggagatagt gccgcggcat ataccatgcg atattatcgc
     4201 ccggaaggat ggcatcatca ggcaggtcat cgcaaaagaa ggtatcgagg cagtagcgga
     4261 aggagatact gtgaaaaagg ggcaggtgct tatatccggc cgtgtacctg
//
LOCUS       DAOSCC010000205         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12898_length_4299_cov_5.075401, whole genome shotgun sequence.
ACCESSION   DAOSCC010000205 DAOSCC010000000
VERSION     DAOSCC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_4299_cov_5.075401"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLG72_09720"
     CDS             complement(<1..323)
                     /locus_tag="PLG72_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HOJ81113.1"
                     /translation="MRKIRDLLTGKIENIRRERISNTTLLMTITVCTFFGMYLLAMAI
                     WKGGFLNPQQFLDLFNNNAYLLIIACGLTMVMISGGIDISGGGITALVTMVCVVYLED
                     HGGTV"
     gene            complement(395..1465)
                     /locus_tag="PLG72_09725"
     CDS             complement(395..1465)
                     /locus_tag="PLG72_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ81114.1"
                     /translation="MGDGIKKFMRSQLVIPLFSLFLLVIFNLLRDPEFFSISLATNNQ
                     GYRVLSGNLISIFNGAAAIAIISIGMTLITSCCKGQDISVAAVAAIAGSVFVSVLRSH
                     EITIPVIILAFLLSCLVSVLFSLFNGVLVAVFKIQPMIATLILFSAGRSIAYWINGGA
                     TPTVSSDFLASIGGFIPGIPIPTPIIIMVVFLVLISIVLRLTTLGLYTQVVGINEGTA
                     HLNGINPVWVKLIAFIISGVCVATAGCITVCRIGLINHETLLLDAEMDVILAVAIGGT
                     SLGGGKFSLFGSVIGAYIIQALTVTLYAMKVPSAAVKAYKAAVIILIVIISSPVVKEF
                     FINLYKKLFKKQVDAKIPIKAS"
     gene            complement(1458..2984)
                     /locus_tag="PLG72_09730"
     CDS             complement(1458..2984)
                     /locus_tag="PLG72_09730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ81115.1"
                     /translation="MQDNTVLSMRNISKSFPGVRALQNVDFTLRKGEIHALMGENGAG
                     KSTLIKVLTGVYPKDSGQIYLSLNGGMKEINVRSPHEAQELGISTVYQEITLCPNLTV
                     AENMFIGRGNYSLVNWRTMKKKAAEILEKLNIPARATQQLDTCSIAVQQMVAIARAVD
                     MDCKVLILDEPTSSLDEQEVEKLFSLMRDLKSRGVGIIFVTHFLEQMYEISDMVTVLR
                     NGELVGEYEVKDLPRVELISKMMGKDLDDVSTIQKRKQPDKTPDALPVYEAVGLSSTN
                     CPNAFDFNISKGEVNGFTGLLGSGRSESVRAIFAADRITGGKIKINGKDVKIRRPKDA
                     MMHGVGYLPEDRKRDGIVEDLSVRENIILALQVMKGFFRPFSRAEAEAFADKYIKLLG
                     IKTASMDTPIKSLSGGNQQKVILARWLLTNPQYLILDEPTRGIDVGTKTEIQKLILKL
                     AEEGVSVTFISSEISEMLRTCSRLIVMRDRKMVGELEGEEMTQDNIMQMIAGEAEQNG
                     "
     gene            complement(3242..4237)
                     /locus_tag="PLG72_09735"
     CDS             complement(3242..4237)
                     /locus_tag="PLG72_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ81116.1"
                     /translation="MKRILTLILVAVLVLTTLAACGQQSANTSKSTSSGSGKLIKVGI
                     VNNPPSESGYRAANVADFEKVFSKENGYDVSTFYSLKNDEQLNAAQQFITDGVDYLLI
                     SAAATDGWTNVLENAKKAGIKVFLFDRMMNVDESLFEAAVISDMAAEGKMAVQWLLDQ
                     KLPEYNVVHIQGAMGSDAQIGRTKALDEQFANGTMKKVIQQTATWDEATAKQIVESVI
                     NSGEKFNVIYAENDGMAKGAVAALDEAGITHGKNGDVVIMGFDCNKWALREVLAGNWN
                     YDGQCSPFQAQKIHEMIQTLEKGGTLSQKVVINEERYFDCNSITEADIDTYGIGE"
BASE COUNT         1019 a         1119 c          980 g         1181 t
ORIGIN      
        1 aatacggttc cgccatggtc ttcaagataa acaacacaga ccatggtcac aagagcagtt
       61 atacctccgc ctgaaatgtc gatccccccc gagatcatga ccatggtcag tccgcacgct
      121 attataagca ggtacgcatt gttgttgaaa aggtcaagaa actgctgggg gttcagaaaa
      181 ccgcccttcc agatggccat cgcaagcagg tacatgccga agaaggtaca aacggttatc
      241 gtcataagca gagtggtatt ggatatccgt tctctgcgta tattttcaat ttttccggtc
      301 aacaaatccc taatttttct catagcatcc tttccccatt actccgtcgg ttttatgcca
      361 gcctggtggc attgcggcag gcttcgaata ccgttcagga agccttgatc ggaatttttg
      421 catcaacctg ctttttgaat aatttcttgt acaggttgat gaaaaattct ttcacaaccg
      481 gcgaactaat tatgactata agtatgataa cagccgcctt ataggccttt accgctgcag
      541 aagggacctt catcgcataa agggtgaccg taagggcctg gattatgtac gctcctatca
      601 cagagccaaa caggctgaat tttccgccgc ccagggatgt cccgcctata gcgacagcca
      661 ggatgacgtc catttccgca tccagcagca gggtttcgtg gttgatgagc cctatcctgc
      721 ataccgtaat gcatcccgca gtggcgacgc aaactccgga tataatgaaa gctatcagtt
      781 tcacccaaac aggattgatc ccgttcaggt gcgcggtccc ttcatttatt cctaccacct
      841 gcgtatataa tccgagagtg gtcagcctta agacgattga aatcaggacc aggaacacaa
      901 ccattataat gatcggggtc ggtatcggga tacccgggat aaaacctcct atactcgcga
      961 gaaaatcgct tgaaacagtc ggggtcgccc cgccgtttat ccagtaagca atggaccgcc
     1021 cggctgagaa caatatcagc gtcgcgatca tgggctggat tttgaatacc gcgaccagga
     1081 ctccgttgaa taagctgaaa aggacggaaa ccaggcagct taataagaat gcgaggataa
     1141 ttaccggtat cgtgatctcg tgcgaacgaa gcacgcttac aaatacgctg cccgctattg
     1201 ccgctactgc cgcgacgctt atatcctgtc ccttacagca tgatgttatc aacgtcatgc
     1261 ctattgaaat aatggcgatt gccgctgccc cgttgaatat acttatcaaa tttcctgaaa
     1321 ggacacgata tccctggttg ttagtagcaa gacttataga aaaaaattca ggatccctca
     1381 acaggttgaa aataacgagc aaaaaaagtg aaaacaacgg tataaccagc tgggacctca
     1441 tgaacttctt aatgccgtca cccattctgt tccgcctccc ccgctatcat ctgcatgatg
     1501 ttgtcctgtg tcatctcctc gccctccagt tcgccgacca ttttgcggtc gcgcatgaca
     1561 atgagccggg aacaggtacg tagcatttcc gatatttccg aagaaatgaa tgtaacgctg
     1621 acgccttcct cggcaagttt cagtattagc ttctgtatct cggtttttgt tcccacgtcg
     1681 ataccgcgcg tcggttcatc caggataagg tattgcggat tcgtgagaag ccagcgggca
     1741 aggatgacct tttgctggtt cccgcctgac agcgatttga tcggagtatc cattgaagcg
     1801 gtctttattc ccagcaattt tatgtactta tcggcaaagg cttcagcctc ggcccttgaa
     1861 aatggcctga agaagccctt cattacctgc agggcaagta tgatgttttc gcgcacggac
     1921 aagtcttcga cgataccgtc gcgtttcctg tcctcgggca aatacccgac gccgtgcatc
     1981 atggcgtctt tgggtcttct gatcttaacg tctttcccgt ttatctttat tttaccgccg
     2041 gtgatcctgt ctgcggcgaa aatagcgcgc acgctttcgc tgcgcccgga accaaggagc
     2101 ccggtaaacc cgtttacttc tccttttgaa atattgaaat caaatgcgtt cgggcaatta
     2161 gtgctggata atcctaccgc ttcgtaaacc gggagggcat ccggcgtttt gtccggctgc
     2221 ttccgtttct gtattgttga aacatcatcc aggtctttgc ccatcatctt cgaaatcagt
     2281 tctacacgcg gcagatcctt tacctcatat tcgcctacca gttcgccgtt cctcagaacg
     2341 gtcaccatat cgcttatctc gtacatctgc tcgaggaagt gcgtaacgaa gattataccg
     2401 acgccccgtg acttgagatc acgcatcagg gaaaagagtt tttcgacttc ctgttcatcc
     2461 agcgaggaag taggctcgtc cagtatgagc actttgcaat ccatgtctac ggcgcgggca
     2521 atggcgacca tctgctgtac ggctatggaa caggtgtcaa gctgctgggt ggcccgggca
     2581 gggatgttta atttttcgag tatctctgcc gccttctttt tcattgttcg ccagttcacc
     2641 aggctgtaat tgccccgccc gataaacata ttctccgcaa cagtaagatt ggggcaaagg
     2701 gtaatctcct ggtaaacggt actgatgccc agctcctggg cctcatgggg cgatctcacg
     2761 ttgatttctt tcataccgcc gttcaggctc aggtatatct gccctgaatc cttcgggtat
     2821 acgcctgtca aaacttttat cagcgttgac tttcctgccc cgttttcgcc cataagggca
     2881 tgtatttcgc ctttcctgag cgtaaaatcc acgttttgca aagcgcggac gcccggaaag
     2941 gatttactta tattcctcat tgacaaaacg gtgttgtcct gcatggcaac catccttaaa
     3001 gtcttttata acgcatcgtt caaagaaaat cctcgattat ggaaaatttt cttacctttg
     3061 cgcatttcaa cgaggcggga gatatgcccg ccgaatgaag ggcaaccgtt acccgctgcc
     3121 tatagaggtg cgggacatca ttcgcctcgt tataaacaac aatgtaagta tgcggaaaaa
     3181 atccgcatac ttgcattgca tgaaccggga catttcaatg gacaagctga acgttcctgt
     3241 attattcgcc tataccatag gtgtctatat ctgcttctgt aatgctattg cagtcgaaat
     3301 atctttcttc attgattacg accttctggc tcagcgttcc gcctttttca agggtctgga
     3361 tcatttcatg gatcttctgg gcctggaacg ggctgcactg gccgtcatag ttccagttac
     3421 ctgcgagcac ttccctgaga gcccacttat tgcagtcgaa gcccattata acgacgtcgc
     3481 cgtttttgcc gtgtgttata cctgcttcgt ccagagctgc aactgcgccc ttggccatac
     3541 cgtcgttctc ggcgtagata acgttgaatt tttcgcccga gttgatgaca gattctacga
     3601 tctgttttgc tgttgcttca tcccatgtag cagtctgctg gatcaccttt ttcatcgtgc
     3661 cgtttgcaaa ctgttcatcc agtgcctttg tacgtccgat ctgagcatcg ctgcccattg
     3721 cgccctggat atgcactaca ttgtattcag ggagcttctg gtccaacagc cactggacag
     3781 ccatttttcc ctctgctgcc atgtcggaaa taacagcggc ttcaaacagg ctctcgtcaa
     3841 cattcatcat acggtcgaag aggaatacct taatgccggc tttcttggcg ttttcaagga
     3901 cattggtcca tccgtcggtt gcagcggccg agatgaggag gtaatccaca ccgtcagtaa
     3961 tgaactgctg ggctgcgttc agctgctcgt cgttcttgag gctgtagaat gtagatacgt
     4021 cgtaaccgtt ttccttggag aaaacttttt caaagtccgc tacgtttgca gcacggtatc
     4081 cggactcaga cggcgggttg tttacgatac ccactttaat gagtttgccg cttccggatg
     4141 aagtgctctt cgaagtgttt gcagattgct gaccacaagc tgccagtgtt gtcagtacca
     4201 acacagcgac cagaatcaag gtcaagattc ttttcatttt ttattcccct tgtacaatta
     4261 atattgtatt aaatcagtga ccgaagccac tgttcgacc
//
LOCUS       DAOSCC010000206         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_12900_length_4299_cov_3.995287, whole genome shotgun sequence.
ACCESSION   DAOSCC010000206 DAOSCC010000000
VERSION     DAOSCC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12900_length_4299_cov_3.995287"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="der"
                     /locus_tag="PLG72_09740"
     CDS             <1..1007
                     /gene="der"
                     /locus_tag="PLG72_09740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ81117.1"
                     /translation="MLRKTSKHVILVANKVDNIGDPPPEVYEFYNLAMGDVETVSSIH
                     GLGMGDLLDRIYSFLPDEKQDDTEEDVIKVAVVGKPNSGKSSLVNRITGEERVIVSDI
                     PGTTRDAIDTYVERNGGKFVLIDTAGIRRKNKITENIERYSVLRSWTAIERSDVCVIM
                     IDAQDGVTEQDAKIAGYAHEQGKASVIVINKWDLIEKGTGTMEEYRKKIMEKLSFMQY
                     APVLFISVKTGQRVHRLLELVKFVYEQASLRISTGMLNDLLNEATAMVQPPSDKGRRL
                     KLFYAVQSAVRPPTFIIFMNDTELFHFSYERYLENQLRKSYGFEGTPIRFIPRERNED
                     "
     gene            1089..1688
                     /gene="plsY"
                     /locus_tag="PLG72_09745"
     CDS             1089..1688
                     /gene="plsY"
                     /locus_tag="PLG72_09745"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOJ81118.1"
                     /translation="MYIRTALSILAGYLLGSLNTSLIVGRFYNVDVRKHGSGNAGMTN
                     TLRTLGKKAALFVVLGDIVKGILAYLAGYYIYGGQTGGLFGGTACIFGHIWPVFFRFR
                     GGKGVLTAFAVLLMFDWKIALGLFAVFAIILLATRYVSLGSITAAFLFPVAAAVFGRG
                     TQTLIFSGIIAVIVIAKHHENIKRLLSGTESKFSLGKGK"
     gene            1702..2727
                     /locus_tag="PLG72_09750"
     CDS             1702..2727
                     /locus_tag="PLG72_09750"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOJ81119.1"
                     /translation="MGRKVSVLGAGSMGTAVSVMLAGNGHNVLMWSLYKDEVDMINEL
                     REQKDKLPGVTVPESVRCTNDLEEAVRFSDICILVIPSQKVRENVRAIASLKYRPLVI
                     SCFSKGLEKGSGLRMSEVIKEELPDVTVVAMSGPCHAEELSKGIPTAYVAASGSRKAA
                     EMIQDLFMSPMFRVYTNPDIIGVELGGAIKNVIALCAGISDGLGYGDNTKAALMTRGI
                     AEMSRLGCAMGAKRETFSGLAGIGDLIVTCTSMHSRNRRAGILIGKGMPVDKALEEVK
                     MVVEGVYTTEPVYNLSKKLGIRMPITAEAYAILFEGKDPGSAVSALMSRDRTCEHEAK
                     DPDDIWK"
     gene            2852..3451
                     /locus_tag="PLG72_09755"
     CDS             2852..3451
                     /locus_tag="PLG72_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOJ81120.1"
                     /translation="MVFYCPNPVPFAWKPYVSSPGKYSPYPMLARGPDTSCPKGYNAA
                     YAILVAGPDYPSITGLVTFTDTPSGTMVSADVRGLPEYRPAAGDKSPVGPFGFHLHEG
                     CICEVGDPEKPFEGCGGHWNPTNQPHGNHAGDFPVLVASKGRARMCFITDRFTVDEIT
                     GRAVIIHENPDDYRSQPAGNSGRRIACGSIRPWDPYDRI"
     gene            complement(3514..>4299)
                     /gene="apgM"
                     /locus_tag="PLG72_09760"
     CDS             complement(3514..>4299)
                     /gene="apgM"
                     /locus_tag="PLG72_09760"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOJ81121.1"
                     /translation="RFHAGISYRHCMVWHGGTGARKLTPPHDILGKHIAPYMPSGDNS
                     DMFIGLMKKSHEILAGHPVNKSRISRGLRPANSIWLWGEGRKPALPGFAGKYGLSGSV
                     IAAVDLIKGIGICAGLDIVEVEGATGNINTNFRGKAEAALKELNSGNDFVYIHIEAPD
                     ECGHRFEIENKVRSIELIDELVVGTLLEGLADYDDFSIMVLPDHPTPLSLRTHTADPV
                     PYVIYRKSCSKPSGVAGYDEFEAQKTGIFIEHGHKLMDRFLQK"
BASE COUNT         1136 a          993 c         1189 g          981 t
ORIGIN      
        1 ccatgctgcg gaagaccagc aaacatgtga tcctggtagc caacaaggtc gacaatatag
       61 gggatccgcc gccggaagta tatgagttct acaaccttgc catgggcgac gtggaaacgg
      121 tgtcttccat ccacggtctt ggcatgggcg atctgctgga caggatatac agttttctgc
      181 ccgatgaaaa acaggacgat accgaagaag atgtcataaa agtggctgta gtcgggaagc
      241 ccaactcggg gaagtcttcc ctggtcaaca ggataaccgg cgaagagaga gtgatagtga
      301 gcgatatccc cgggaccaca agggatgcta tagacacgta tgtggagcgg aatggcggca
      361 aattcgtgct catagatacc gcaggcatac gcaggaaaaa caaaataacg gagaacatcg
      421 aaagatacag tgtgctgcgt tcgtggacag caatagagag gtctgacgtc tgtgtcataa
      481 tgatagacgc gcaggacggc gtaacggaac aggacgcgaa gatagccgga tacgcccatg
      541 agcaggggaa ggcatcggta atcgtcataa acaaatggga ccttatcgaa aaaggtaccg
      601 gcacgatgga agagtatcgc aagaagatca tggagaaact gagcttcatg cagtatgcgc
      661 ctgtgctgtt catctccgtg aagaccgggc agagggtgca caggctgctg gaacttgtga
      721 agttcgtata tgaacaggca tcactcagga tttcgacagg tatgctcaat gatttgctga
      781 atgaagccac agccatggtc cagccgccgt ccgacaaagg caggaggctg aaactgtttt
      841 atgctgtaca gtcggcagtc aggccgccta cattcattat cttcatgaat gacaccgagc
      901 ttttccactt ttcctatgaa cgttacctgg aaaaccagct aaggaaaagc tacggatttg
      961 aaggtacgcc gataaggttc atacccaggg agcggaatga ggattgacct ggtcagacgc
     1021 agtctgcaca ggcattttgc gtacagaccg gcaatgaatc aaaatactgg catatggggg
     1081 gatcgcgcat gtatatccgg acagcgcttt caatattagc cgggtacctg ctgggaagcc
     1141 tgaacacatc gctgatagta ggcagattct acaatgtgga tgtgagaaag catggcagcg
     1201 ggaacgcagg catgacgaat acgctgagga cgctcggcaa aaaagctgca ttgttcgtgg
     1261 tgcttggcga catagtaaag ggtatcctgg catatcttgc aggttattac atatacggcg
     1321 gtcagaccgg cgggctgttt ggcgggaccg catgcatatt tggacatatc tggcctgtct
     1381 tcttcaggtt caggggagga aagggagttc tcacggcatt cgccgtgctg ctcatgttcg
     1441 actggaagat cgcccttgga ctatttgcgg tttttgccat aatattgctg gccacgcgat
     1501 atgtttcgct gggttccatc acagctgcat ttctttttcc tgtggctgca gcggtttttg
     1561 gcagggggac ccaaacgctg atattttcag gcataatcgc cgtgatcgtt attgcaaaac
     1621 accacgagaa catcaaaagg ctcctcagcg gtacagaatc gaaattttct ctaggaaaag
     1681 ggaaataacg gagggatcgc tatgggaagg aaagtttctg ttcttggagc aggaagcatg
     1741 ggtacggctg tatccgtgat gcttgccgga aacggtcata acgtgttgat gtggtcactg
     1801 tacaaagacg aagtcgatat gatcaatgaa ctaagggaac agaaagacaa acttccagga
     1861 gtgaccgtcc cggagagcgt aagatgcacc aacgaccttg aggaagcggt gcggttctcg
     1921 gatatatgta tcctcgtgat accatcgcag aaagtaaggg aaaatgtacg ggctattgct
     1981 tcgttgaaat acagaccgtt ggtgataagc tgcttttcaa aaggactgga aaaaggttcg
     2041 ggactgagaa tgtccgaagt gataaaggaa gaactgccag atgtcacagt tgtagcgatg
     2101 tccgggccat gccatgcgga agaactttca aaagggatac ccaccgcata tgttgccgcc
     2161 tccgggagca ggaaagccgc tgaaatgata caggacctgt tcatgtcgcc catgttcagg
     2221 gtatatacga atcccgacat catcggtgtc gaactcggag gggcgataaa aaacgtaata
     2281 gcgttatgtg ccgggatatc cgacggcctt ggttacggag acaatacaaa agctgcgctg
     2341 atgaccaggg gcatagcgga gatgtccagg ctcgggtgcg ctatgggagc taaaagagaa
     2401 accttttcag gtcttgccgg tatcggggac ctgatcgtga catgtacgag catgcacagc
     2461 aggaacaggc gtgcagggat actcatcggg aagggcatgc ctgtggataa ggcactggaa
     2521 gaagtaaaaa tggtcgtgga aggagtttac acgacggaac ctgtctataa cctttcaaaa
     2581 aaacttggga tcaggatgcc catcactgcc gaggcgtatg ctatcctgtt cgaaggaaag
     2641 gatcccggca gcgctgtatc cgcattgatg agcagggacc ggacctgcga gcacgaagca
     2701 aaggatccgg atgatatctg gaaatgatcc aaaggacctg gcaggcaaac cttggatcca
     2761 cccgccaaaa cattttaaca ggcaactttt cagagttatt tacataatat ggtatagcga
     2821 gcaattatga tcgtatccgg agggtgcgaa catggtattt tattgtccga atcccgtacc
     2881 gttcgcctgg aagccgtatg tctcatctcc agggaaatac agtccatacc ctatgcttgc
     2941 caggggtccg gatacatcgt gccccaaagg gtacaacgct gcgtacgcga tactcgttgc
     3001 cggtcccgac tacccgtcga taaccggcct tgtaacattt accgacaccc cgtccggcac
     3061 catggttagt gctgatgtaa gaggattgcc tgaatacaga cctgccgccg gtgataaaag
     3121 cccggtgggg ccttttggat tccacttgca tgaaggatgc atatgtgaag tcggagatcc
     3181 tgaaaagccc ttcgaaggct gcggaggaca ctggaaccca acaaaccagc ctcacggcaa
     3241 tcatgcggga gactttcccg tattggtggc aagcaaaggc agggcaagga tgtgctttat
     3301 cacagaccgt ttcactgtcg atgaaataac aggcagggcg gttataatac atgaaaaccc
     3361 tgatgattac aggtcccagc cagccgggaa ttcaggcagg cggatcgcgt gcggcagcat
     3421 caggccgtgg gatccatatg acaggatata gaaaaaacgg gctgccctta ttgttttgta
     3481 aatgaataaa gccggtgaca tcaccggctt acattatttc tgaagaaacc tgtccataag
     3541 tttatggccg tgttcgatga atatccctgt cttttgcgct tcgaattcat catagcctgc
     3601 aacgcctgac ggctttgagc agctcttcct gtaaatgaca tacggtacag ggtctgccgt
     3661 atgcgtcctc aatgaaagcg gagtcgggtg atcaggaagg accatgatgc tgaagtcgtc
     3721 ataatctgca aggccttcca gcaaagtgcc gacgacaagt tcgtcgatca gttcgatgga
     3781 tctcactttg ttttcgatct caaacctgtg gccgcattcg tccggagcct ctatatggat
     3841 atatacgaaa tcatttccgc tattgagttc cttcagcgca gcctcggctt ttccgcggaa
     3901 attcgtgttt atattgcctg tggcgccttc gacttcgacg atatccagtc cggcgcagat
     3961 acctattcct ttgatgagat cgacggcagc gataacagag ccgcttagtc catatttgcc
     4021 ggcaaaaccg ggcagtgccg gtttcctgcc ttcgccccag agccatatcg agttggcggg
     4081 acgaagccct cgtgatatcc ttgatttgtt gaccggatga ccggccagta tttcatggct
     4141 ttttttcata aggccgatga acatatcact gttgtcgccg gaaggcatat atggagctat
     4201 gtgttttccc aggatatcat gcggaggcgt aagcttcctg gcgcctgttc cgccatgcca
     4261 caccatgcag tgcctgtaac ttataccggc atggaacct
//
LOCUS       DAOSCC010000207         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13094_length_4253_cov_4.821582, whole genome shotgun sequence.
ACCESSION   DAOSCC010000207 DAOSCC010000000
VERSION     DAOSCC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13094_length_4253_cov_4.821582"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLG72_09765"
     CDS             <1..460
                     /locus_tag="PLG72_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81122.1"
                     /translation="IFGVMAVMFHSMTKSAIALAAASAALGAIMYETGAIWSALLEIS
                     VCSGLVAVIFISAISLSNMDKKELEKVYEDKKRMSLLPVVLILSGAVLVIAALSAGVS
                     LPHADASAKAAGDLREILWNNRQADIWGQIIVMITGAAAVSVLFRESDWK"
     gene            457..765
                     /locus_tag="PLG72_09770"
     CDS             457..765
                     /locus_tag="PLG72_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOJ81123.1"
                     /translation="MNAIGNIVFIVGFLLIIAGCYCLIRTYHMLKIILGIEVAMKAVT
                     LFITYAGYINGNIDLAQSFVITSIVIEVVVAVVAAGIAINLYRKYGSMDIRNLRKLKG
                     "
     gene            779..1099
                     /locus_tag="PLG72_09775"
     CDS             779..1099
                     /locus_tag="PLG72_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81124.1"
                     /translation="MGVLILAPPITFLIFVAIFFAFSRGFSQYSHESPRGEGGLDPYA
                     CGQRDVRNYVNPDYSQFYPFAFLFTIMHVLVLVAATAPYDAPVLPVVFIAAGILCLIM
                     IFKR"
     gene            1106..1561
                     /locus_tag="PLG72_09780"
     CDS             1106..1561
                     /locus_tag="PLG72_09780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOJ81125.1"
                     /translation="MGLLQRAIEWGTRKSPWIIHFNAGACNGCDIETIDALTPRFDME
                     RIGVLQEGSPRHADILVCTGAVTLQTRDCLKQIYDQMPEPKYVIAIGTCACTGGIFDG
                     CYCVMGGIDTVIPVDVYIPGCAVHPEAIISAVRKLLDGKKGGGRHADRK"
     gene            1545..2063
                     /locus_tag="PLG72_09785"
     CDS             1545..2063
                     /locus_tag="PLG72_09785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOJ81126.1"
                     /translation="MQTENEIVGKLCGKFGFLQDKVHVQRQKRIFTEFLAKEEFEQVL
                     HYAHDELGFYRASHVVGTDEGDDLGFIYLLSDENGVILALKEKAPKSDPVINSMTDLY
                     PSLEQHERELVDLFGAVVEGLPEGPSYPLPDGWPKGSYPMRKDWDPKYFDRNTMTYDP
                     PSDDVKKEGGNK"
     gene            2060..3289
                     /locus_tag="PLG72_09790"
     CDS             2060..3289
                     /locus_tag="PLG72_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOJ81127.1"
                     /translation="MSKKRRNRIELPIGPQHPSLKEPATFWITLEGEKVVNAVIGVGY
                     NHRGLEKACESRNYIQNLYLIERVCGICSHTHSTAFCQGIEELAGITIPRRAAYIRVI
                     VAELERLHSHLLWLGVAAHEIGFDTMFMYTWRDREKVLDILAEVCGNRVNYGINTIGG
                     VRRDVDEEMLGNMSNVADELENQIKYYLDLALTETTLSARLAGVGKLSREDALRFGVA
                     GPVARSAGIADDIRVKEPYAAYSEIPVNPVTDTNADVLGRTVVRIKEMLESLNIIRFC
                     IKNLPDGDIAVKAPLRIPAGEVISRTEAPRGENIHYIRSNGTDMPDRVRVRAPSEANW
                     RGVKHMLEGGYLADVPITIAAIDPCYSCTDRAIVIDSGSGTEVMDWQRLRQYSIDWYK
                     KQGINVSDVKIPERKYY"
     gene            3387..>4253
                     /locus_tag="PLG72_09795"
     CDS             3387..>4253
                     /locus_tag="PLG72_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOJ81128.1"
                     /translation="MLLSLFKILVFPGLIFLAVYALLCEYIDRKVYARLQNRMGPPWY
                     QPLADFLKLLGKETIIPADANRSMFQILPVISLSAVAAAFIYVPVLGASAVYSFEGDL
                     IVVLYLLTIPTMCEFLGGWYSRSVYSTIGSVRTLTQMFAYEVPLFMALLAPALIAKTW
                     SVTGMTSFYSEHPLYVLFNIPAFLTALIAVQGKLERAPFDSPEAETEIVSGALVEYSG
                     KLLALFRMSMDCELVVMASLISAIFLPFMTGIVILDFILYFAKTLAVLLVLTLMRAAM
                     ARLRVDQMVNFCW"
BASE COUNT         1031 a          980 c         1237 g         1005 t
ORIGIN      
        1 aattttcggg gtaatggccg ttatgttcca tagcatgacg aaatccgcca tagccctggc
       61 cgcagcaagc gcagcgcttg gagcgataat gtatgaaacg ggcgcgatat ggtcggcatt
      121 gctcgagatc tccgtttgct caggtctggt ggcggtaata ttcatcagcg cgataagttt
      181 gtcaaacatg gataaaaaag agcttgagaa agtttatgag gacaagaaac gtatgtcact
      241 gctgccggtc gtcctgatac tcagcggggc tgtattggtc attgccgcgt tgtcggcggg
      301 tgtttctctg ccgcatgcgg atgcttcagc caaagcagcg ggcgatctgc gtgagatatt
      361 gtggaacaac cgccaggccg acatctgggg gcagatcatc gtcatgataa ccggtgcggc
      421 cgccgtttca gttttgttca gggagagtga ctggaaatga atgcaattgg gaatatagtc
      481 ttcatagttg gatttttgct gataatagcg ggatgctact gcctcatacg cacgtatcat
      541 atgctcaaga tcattttagg catagaagtg gcaatgaaag ccgttacgtt atttatcacg
      601 tatgcagggt atattaatgg aaatatcgac cttgcacagt cattcgtcat tacatcgatc
      661 gttatcgagg tcgttgttgc cgttgttgcg gcagggatag ccattaacct ttaccggaaa
      721 tacgggagca tggatatccg caatttaagg aaactgaaag gatagcaggt ggaatgttat
      781 gggtgtcctt atacttgcac cgccgatcac ttttttgata ttcgtcgcta tattcttcgc
      841 attctccagg ggcttttcgc agtattccca tgaaagcccc aggggcgagg ggggactgga
      901 tccgtatgcc tgcggccagc gtgatgtcag gaactacgtc aatcctgact acagccagtt
      961 ttacccgttc gcatttttgt ttacgatcat gcatgtactg gtgctcgtgg cagcgacagc
     1021 tccttacgat gcgccggtcc tgcctgttgt gttcatagcg gcagggatac tttgcctgat
     1081 aatgatattc aagaggtgaa aggctttggg acttttgcag agggcaatag aatgggggac
     1141 aaggaagtcg ccctggataa tacactttaa tgcgggcgcg tgcaacggct gcgacataga
     1201 aacgatagat gcgctgacgc caaggttcga catggaacgc ataggcgtcc tgcaggaggg
     1261 cagcccgcgg cacgcagaca tattggtatg taccggcgcc gtcacccttc agacacggga
     1321 ttgtcttaaa cagatatatg accagatgcc tgagccgaag tatgtcatag cgataggcac
     1381 atgcgcctgt acgggcggga tattcgacgg ctgctattgc gtaatgggcg gcatcgatac
     1441 cgtgataccc gtggatgttt atatacctgg atgcgcagtg catccggaag cgatcataag
     1501 cgccgtccga aagctcctgg acgggaagaa aggaggcggc aggcatgcag accgaaaatg
     1561 agatagttgg gaaactatgc ggaaaattcg gatttttgca ggacaaagtg catgtgcaga
     1621 ggcaaaagcg catatttacg gagttccttg caaaagagga gttcgaacag gtgctgcatt
     1681 atgcgcatga tgaactggga ttctacaggg ccagccatgt tgtcgggaca gatgaaggag
     1741 acgacctcgg attcatttac ctgctgtccg atgaaaacgg ggtcatcctg gcgcttaagg
     1801 aaaaagcgcc gaaatccgac ccggtgatca attcgatgac ggacctgtat ccttcgctgg
     1861 aacagcatga acgggaactg gtggatctgt tcggcgccgt tgtcgaaggc cttcccgaag
     1921 gcccgtcata cccgctgcct gacggatggc cgaagggaag ctaccctatg cgcaaggact
     1981 gggatcccaa atatttcgac aggaatacga tgacatatga tccgccgtca gatgatgtga
     2041 aaaaggaggg cggaaacaag tgagcaaaaa aagacgcaac aggatagaat tgccgatcgg
     2101 tccgcagcat ccttcattga aggaaccggc aactttctgg ataacgcttg agggggaaaa
     2161 ggtcgtaaat gcggtcatag gcgtgggcta taaccacagg gggctggaga aggcctgcga
     2221 gagcaggaac tatattcaga acctgtacct gatcgagcgt gtgtgcggca tatgctcgca
     2281 tacgcacagt accgctttct gccaggggat agaggaactg gccggtatta cgataccgcg
     2341 gcgtgccgca tatatccgcg tgatcgtggc agaactcgag cgcctgcaca gccatttgct
     2401 ctggctggga gtcgcagcgc atgagatcgg tttcgatacg atgttcatgt atacctggcg
     2461 cgaccgtgaa aaagtccttg acatactggc cgaagtctgc gggaatcgcg tgaattacgg
     2521 catcaatacc atcggcggcg tacggaggga cgtagatgaa gaaatgctcg ggaatatgtc
     2581 gaatgtggcg gatgaactgg aaaaccagat caaatactac cttgatcttg cactgacgga
     2641 gacaacgctt tctgcaaggc ttgcaggcgt tggcaagctt tcgcgcgaag atgcgctgag
     2701 atttggggta gcggggccgg tggcacggag tgcgggcatt gccgatgata tccgtgtcaa
     2761 ggagccttac gcggcataca gcgagatccc ggtcaacccc gtgacggata cgaacgcgga
     2821 tgtcctgggg cgtacggtgg tacggataaa agagatgctt gaaagcctga atatcatacg
     2881 attctgcatc aaaaacctcc ctgacggcga tatcgcggta aaagccccgc ttcgcatccc
     2941 tgcaggcgaa gttataagca ggacggaagc gccgcgcggc gagaatatac actatatacg
     3001 gtcaaatggc accgacatgc cggacagggt gcgggttcgc gcgccaagcg aagccaactg
     3061 gcgcggtgtt aagcacatgc tggaaggcgg gtatcttgcg gatgtgccga tcaccattgc
     3121 tgccatagac ccatgctatt cgtgcacaga ccgcgccatc gtcatcgatt ccggaagcgg
     3181 gactgaggtc atggactggc aaaggctcag gcagtacagt atagactggt ataaaaagca
     3241 gggcataaat gtgtccgatg taaaaatacc ggagcgcaaa tattactgat ggttgtatga
     3301 tagtgctgaa cgatggatga ctttactgac agatactgga tcgacactga aaccgaaatc
     3361 attactggag agcaaagagg aacggtatgc ttttgagttt gtttaaaata cttgtatttc
     3421 cggggttgat atttttagcc gtatatgctt tgttatgcga atacattgac aggaaggtat
     3481 atgcaagact gcagaacagg atgggcccgc cgtggtacca gcctcttgcg gacttcctga
     3541 agctgctcgg caaggagacc attatccctg ccgacgcgaa caggagcatg ttccagatcc
     3601 tgcccgtgat atcgctttcc gcggtggcgg ctgcattcat ttatgtgcct gtactgggcg
     3661 catccgccgt ttactcgttt gagggcgacc ttatcgtggt gctgtatctc ctgactatcc
     3721 ccacgatgtg cgagtttttg ggcgggtggt attcaagaag cgtatattcc acgatcggtt
     3781 cagtacgcac gctgacacag atgtttgcgt acgaggtacc gcttttcatg gcgcttcttg
     3841 ccccggcgct catcgcaaaa acatggtcag ttacgggaat gacgtcgttt tactcggaac
     3901 atccgctgta tgtcctgttc aatatcccgg cgtttctcac ggcgctgatc gcagtgcagg
     3961 gtaagcttga acgggctcct ttcgactcgc ctgaggccga gacagagatc gtatccggcg
     4021 cactggttga atacagcggc aagctcctgg cgttattcag gatgtcgatg gattgtgaac
     4081 ttgtggtgat ggcttcgctt atctcggcga ttttcctgcc tttcatgaca gggatcgtta
     4141 tactggattt catcctgtat ttcgcgaaaa cgcttgccgt gctcctggtc cttacactga
     4201 tgcgcgctgc aatggcacgg ctcagggtag accagatggt gaacttttgc tgg
//
LOCUS       DAOSCC010000208         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13141_length_4242_cov_3.737043, whole genome shotgun sequence.
ACCESSION   DAOSCC010000208 DAOSCC010000000
VERSION     DAOSCC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13141_length_4242_cov_3.737043"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLG72_09800"
     CDS             complement(<1..698)
                     /locus_tag="PLG72_09800"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOJ81129.1"
                     /translation="MMQDNMQPMQEMDVAISSRVRLARNLKAYPFSTRMTRAQGAEIL
                     EKVKKAIFSDNDPGFSGGFTYYGMNTMKPLDRQLLVEKHLISPEFAEGQAERAAIISR
                     DERVSILVNEEDHLRIQCIYPGMQFTEAWRRCDELDTRLDAKLGFAFDKINGYLTCCP
                     TNVGTGIRASVMLHLPALSMTGYIKSILETCSKIGVAVRGAYGENSEASGNMFQISNQ
                     VTLGQTEDEIIAGI"
     gene            complement(700..1194)
                     /locus_tag="PLG72_09805"
     CDS             complement(700..1194)
                     /locus_tag="PLG72_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOJ81130.1"
                     /translation="MREANVHYTQIINGNKVEMYLCSQCAEEKGKFTFSPQLTLGSLL
                     WGFGLGGGDDYPGIGQQHVLRCSVCGMSFDDFRRLGKLGCPNCYSVFRDNLEPILRRL
                     HGGIEHTGKVPANLADEIKTTNEIERLKAELSAAIENEQYEKAAELRDRIRELENSGK
                     NGGV"
     gene            complement(1303..1761)
                     /locus_tag="PLG72_09810"
     CDS             complement(1303..1761)
                     /locus_tag="PLG72_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOJ81131.1"
                     /translation="MARMSDIIEEFIKQLINETDGVIEIQRNELANYFKCVPSQINYV
                     IDTRFTTERGYYVESRRGGGGYIKIKRVNVDRPGNYLMHIVSSMGDSISQQSAEVFIN
                     NFVDYEVINEREGLLLKAATSDKALGGIGAPDRDIIRASILKNMLTALLT"
     gene            complement(2055..3146)
                     /locus_tag="PLG72_09815"
     CDS             complement(2055..3146)
                     /locus_tag="PLG72_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOJ81132.1"
                     /translation="MKRKLLVVLLVAALVVSTAAGCASKKENSAMDTAAPSHQSTSAG
                     GSSSVRGDYNTAEVPSEGVTAVDVEFEKADSLTGIGNLTDDITNPILDERKIIRSANL
                     TIEVENFDTAFSSIETIITGIGFVQETNINTDRVYDNGEVKFYRSGTIVLRVDRSRFD
                     SVLNKLRGIGDVYNYTTTGEDVTEQYYDIESRLRLLKLEQEKIEAYLAKLSDLDEIFK
                     AESKLTDIRYQIESLTGKLNKLSSLVDLSTITINLYEKRPGSEAKPLTYGEKLLESLK
                     ESLLNVVEFLGDLLLFIVSAIPALILLGLLILIGVAVYRRITRNRRQGAGIAGAAAVP
                     ARPEGQQGRQEPPAAKQEATQTRQEDSNQ"
     gene            3482..>4242
                     /locus_tag="PLG72_09820"
     CDS             3482..>4242
                     /locus_tag="PLG72_09820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ81133.1"
                     /translation="MEVRPIKPDEKTAFDKIQTIAFMFKRDFSKEEKEGAAPDSAQQL
                     VNDGYKTGRAAFDDSGKMCSCFDLIPYEVVFDGNIVKMGGIGGVASLPEERDKKYVRH
                     IFEYVMNEMYENGYVFSYLYPFSHIYYRKFGYEHNMTAANYSIPISSLKQFAQTGRMS
                     MAADDAEKQLIKALYEEFIKDKNLAVTRTDKLWNRRFDNDPYRDSVYLYIWYNSRNEA
                     RGYIQYHSERSGGIKTDFVVRELVWLDTEALKGIFA"
BASE COUNT          951 a         1202 c          932 g         1157 t
ORIGIN      
        1 cttatccccg caattatctc atcctcggtc tgccccagtg tcacctggtt cgatatctgg
       61 aacatgttcc ccgaagcttc gctgttttca ccgtaagcgc ccctgacagc aacgcctatc
      121 ttgctgcatg tctccaggat gcttttgatg taccccgtca tcgacagcgc gggcaggtgc
      181 aacataacgg atgccctgat ccccgttccc acgtttgtag gacagcatgt caggtatccg
      241 ttgattttat cgaacgcgaa cccaagcttc gcatccagcc tggtatcaag ttcatcgcac
      301 cgtctccaag cctctgtaaa ctgcatgccg gggtaaatgc actgtatcct cagatggtct
      361 tcctcgttca ccagtatgct tacccgctcg tccctgctta tgatggcagc ccgctcggcc
      421 tggccttcag cgaattcggg gctgatcaga tgcttttcaa caagcagctg cctgtccagc
      481 ggcttcatgg tattcatgcc gtaatacgtg aatcctccgc tgaaacccgg atcattgtct
      541 gaaaaaatgg ctttttttac tttctccagt atctctgctc cctgggcacg ggtcatgcgc
      601 gtcgagaacg gatatgcctt aaggttcctt gcaagtctca ccctgctgct gattgcaaca
      661 tccatctcct gcatcggctg catgttgtcc tgcatcatct cacacacctc cgttctttcc
      721 gctgttttcg agttccctga tcctgtccct cagctctgcc gccttctcat attgctcatt
      781 ttcaatggca gcagacagtt ccgccttgag tctctctatc tcattggtcg tctttatttc
      841 atccgccagg ttggcaggta cttttcccgt gtgttcgatg cctccgtgca gcctcctgag
      901 tattggctcc aggttgtctc tgaatacact gtagcaattg ggacagccga gctttccgag
      961 cctcctgaaa tcatcgaaac tcataccgca cacgctgcac cttagtacgt gctgctgtcc
     1021 gatgcccgga taatcgtcgc ctcctccaag tccgaacccc cacagcagac tgcccagggt
     1081 caactgggga ctgaacgtga atttcccctt ttcttccgcg cattggctgc aaaggtacat
     1141 ttcgaccttg ttgccgttga ttatctgcgt gtaatgcacg ttggcttctc tcatgtgaca
     1201 ctgctggcat aacatatgcg tcactccctg taaatatccg aattggaatt attaccacaa
     1261 aatacattct attaacaaat cacctggctt tgttccgccg gatcatgtca ggagggcagt
     1321 cagcatattt ttcagtatcg acgcccttat tatatccctg tcaggcgcgc ctataccgcc
     1381 aagggctttg tcgctggtcg ccgctttcag caggagcccc tccctttcat ttattacttc
     1441 ataatcgacg aaattgttta tgaaaacttc cgcagactgc tgcgaaatgc tgtctcccat
     1501 cgagctgaca atgtgcatca ggtaattacc cggcctgtcc acattgaccc tctttatctt
     1561 gatgtatccg ccgcctcccc gtctgctttc cacgtagtag cctctttcgg tggtgaacct
     1621 ggtatcgata acgtaattta tctgcgaggg cacgcacttg aagtaattag ccagttcatt
     1681 acgctgtatt tctatgacgc cgtcagtctc gttgatcagc tgctttatga attcttcgat
     1741 tatatcgctc atcctggcca tctgtgctca ctcccttcgt atcatatact gcaaaaacag
     1801 ttattgattt tgactatctt tgactttcat atatattata atccactacg gttttatatg
     1861 caagcctcaa ataccatgtt aacactgcga tccggacagt cccggcaatc gagccagaca
     1921 tgcgcacgaa agcccctgca ggacctgccg gggctttcat tcatttatct tccagagttt
     1981 ttatctttac ggccggtatt ggttccttcc ggctactgtc agtttacccg tggaattttc
     2041 cggttccgcg ccagttactg gttcgaatct tcctgcctcg tctgcgttgc ctcctgtttt
     2101 gccgccggtg gctcctgcct tccctgttga ccttccggtc ttgcgggcac agcagccgct
     2161 cccgcgattc cggctccctg ccttcggttc ctggttatcc ttctgtatac tgcaacgccg
     2221 atcaaaatga gcaatcccag caatatcaat gcaggaatcg ctgaaactat gaacagcagc
     2281 aggtcgccca ggaactccac cacgttgagc aggctttcct tcaggctttc cagcagtttc
     2341 tccccatatg tcagcggttt ggcttcagag cccggccgct tttcgtacag gttgatcgta
     2401 atagttgaca ggtccaccag gctgctcagc ttgttgagct tcccggtcag gctctcgatc
     2461 tggtacctga tatccgtaag cttgctttcc gctttgaaaa tttcatcaag gtcgctcagt
     2521 ttcgccaggt aagcctcgat tttctcctgc tcgagcttca gcagcctgag ccttgactct
     2581 atatcataat actgctctgt tacatcctcg cctgttgtcg tgtaattgta aacgtctccg
     2641 atccccctga gcttgttcag gacggaatcg aacctgctcc tgtcgaccct tagcacgatc
     2701 gtcccgctcc tgtagaactt gacttcgcca ttatcatata cgcggtcggt gtttatattc
     2761 gtctcctgta cgaacccgat acctgttatg atcgtctcaa tgctgctgaa agccgtatcg
     2821 aagttctcga cctcgatggt aaggttggcg ctgcggatta tttttcgctc atccagtatg
     2881 ggattcgtta tgtcatctgt caggttccct atcccggtca gcgaatccgc cttttcaaat
     2941 tcgacatcga cagccgttac cccttcagaa ggcacttccg ccgtgttata atcgccgcgc
     3001 acactggaac tgccgccggc actggtactc tgatgactcg gagcagcagt atccatcgcg
     3061 gaattctctt tttttgaggc acaacctgca gcggttgata ccaccagcgc tgcgaccagg
     3121 agtacgacta acagttttct tttcattttt ataccccctg tcttctttga tcccggatat
     3181 cgggttctgg taataaagac gcaaaaatgc cttcaaagtt ccatggcggg ctatagtata
     3241 catattttgg ctgtcctcac atacatttga tatatcgccg ggcagcatcg gcagcacgcc
     3301 gttagtgtcc cagaaagcag caatccggca aagtcccggc aggctccctg ccaatgacaa
     3361 atcatcagcg aaacagcaga acaaacgtcg gaaacttacc tgttccgcgt ccggttttat
     3421 tcagatataa ttaatttaat taaggaaaaa acagtttggg cgacacggag gaggcggtca
     3481 aatggaagta aggccaataa aaccggatga aaaaacagct ttcgacaaga tccagacaat
     3541 cgcttttatg ttcaaaaggg atttcagcaa agaagaaaaa gaaggcgcag cgcctgacag
     3601 cgcacagcag cttgtaaacg acggttacaa aacaggccgc gcggcattcg acgacagcgg
     3661 caagatgtgt tcatgcttcg acctgatccc ttacgaagtt gtcttcgacg gcaacattgt
     3721 caaaatggga ggcatcggcg gcgtcgcctc gctcccggaa gagagggata agaaatacgt
     3781 aaggcatata ttcgaatatg tcatgaacga gatgtacgag aacggttatg tattctcata
     3841 tctctatccc ttctctcaca tctattacag gaaattcggg tatgaacaca acatgactgc
     3901 cgcaaactac agcatcccga tctcctcgct caaacagttc gcccagacag gccgcatgag
     3961 catggctgcc gatgacgctg aaaagcagtt aataaaagca ctctacgagg aattcataaa
     4021 agacaagaac ctggcagtga cccgcacgga caagctgtgg aacaggcgct tcgacaacga
     4081 cccgtacagg gacagcgtct acctttatat ctggtacaac agccggaatg aagccagggg
     4141 ctatatccag taccactctg aaagatccgg cggcataaaa accgactttg tcgtccgcga
     4201 actggtatgg ctcgatacgg aagcccttaa aggcatcttc gc
//
LOCUS       DAOSCC010000209         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13233_length_4220_cov_5.703721, whole genome shotgun sequence.
ACCESSION   DAOSCC010000209 DAOSCC010000000
VERSION     DAOSCC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13233_length_4220_cov_5.703721"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLG72_09825"
     CDS             <1..339
                     /locus_tag="PLG72_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HOJ81134.1"
                     /translation="RNVNIFAKRMKGGKSWSERGANNLSKIIALKMEKGFKDKISALI
                     SGQLSERLTERFVEALSLTKAAVKKTVKKSLYPIHQGQIPFSNCKVTNGRKAIRSMFD
                     LKVFSEMVYR"
     gene            1049..1360
                     /gene="rpsJ"
                     /locus_tag="PLG72_09830"
     CDS             1049..1360
                     /gene="rpsJ"
                     /locus_tag="PLG72_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ81135.1"
                     /translation="MANNQKIRIRLKAFDHQILDQSAEKIVDTAKRTGAQVSGPVPLP
                     TKKEVFTILRAPHKYKDSREQFEMRTHKRLIDILMPTPKTVDALMRLDLPAGVDIEIK
                     L"
     gene            1425..2063
                     /gene="rplC"
                     /locus_tag="PLG72_09835"
     CDS             1425..2063
                     /gene="rplC"
                     /locus_tag="PLG72_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOJ81136.1"
                     /translation="MSKFILGKKIGMTQVFDENGIVIPVTVIHAGPCAVVQKKTVEND
                     GYTAIKLGFEDVPEKKLNKPEKGLFSKINVTPKKYLREFRTEDIDKFEVGQQINVADM
                     FSSGDRIDVSGISKGKGFQGVMKRFGSSRGPETHGSKYHRRVGTMGAGTSPGRVFKGK
                     KLPGHMGLEKVTVQNLDVVKVDSERNLMLVKGAVPGPKGGLVIVRDTVKAGK"
     gene            2087..2710
                     /gene="rplD"
                     /locus_tag="PLG72_09840"
     CDS             2087..2710
                     /gene="rplD"
                     /locus_tag="PLG72_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOJ81137.1"
                     /translation="MPKVDLYNMKGETVGEIELSDAIFGIDVNENAMYLAVQNQLANR
                     RQGTQSTKTKSEVSGGGRKPYRQKGTGRARQGSIRSAQWIKGGIVHGPKPRSYSYTLP
                     KKLKRLALKSALTSKVNSNDILVLDQLNFDSIKTKQMVNVLDALKVDKGALIVLDSKN
                     ETVVKSARNIPGVKTALVNTINVFDILKYDKFIITKDAVAKVEEVYA"
     gene            2710..3063
                     /gene="rplW"
                     /locus_tag="PLG72_09845"
     CDS             2710..3063
                     /gene="rplW"
                     /locus_tag="PLG72_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOJ81138.1"
                     /translation="MRAPEDVIIRPFITEKSNQEVAAGKYTFIVDVNATKTEIRQAAE
                     KLFQVKVLEVNTVNYSGKEKRLGVHVGIRPSWKKAVIKIDLNPEPEKYLTKGGKEAVS
                     PKKYKTSIEEYGVTQ"
     gene            3207..4040
                     /gene="rplB"
                     /locus_tag="PLG72_09850"
     CDS             3207..4040
                     /gene="rplB"
                     /locus_tag="PLG72_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ81139.1"
                     /translation="MAVKKYSPTSPARRFMTVSTFEEITKKEPEKSLVEILRKTGGRN
                     VYGRITVRHIGGGMKRKYRIIDFKRDKDGINAKVAAIEYDPNRTANIALLHYTDGEKR
                     YIIAPVGLNVGDIVESGPDADIKPGNALPLANIPVGTMVHNIELKPEKGGQLVRSAGG
                     AAQLMAKEGDYAQLRLPSGEVRMVSLQCKATIGQVSNIEHENISIGKAGRKRWMGVRP
                     TVRGVVMNPVDHPHGGGEGKSPIGRPSPVTPWGKPTLGYKTRKKNKKSDKFIVKRRNA
                     K"
     gene            4084..>4220
                     /locus_tag="PLG72_09855"
     CDS             4084..>4220
                     /locus_tag="PLG72_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HOJ81140.1"
                     /translation="MSRSLKKGPFVDQKLLKKIEEMNRANDKKVIKTWSRASTIFPQM
                     VG"
BASE COUNT         1371 a          863 c         1145 g          841 t
ORIGIN      
        1 aggaatgtga acatatttgc aaagcgtatg aagggaggaa aaagctggag tgagagaggg
       61 gcaaacaatt tatcaaaaat catagcttta aagatggaga aaggcttcaa agataaaata
      121 tcagcactta tatcaggaca gctttcagaa aggcttacgg agcgtttcgt agaagctctg
      181 agtttgacta aggcagcagt aaagaaaaca gtgaagaaat cactatatcc aatacatcaa
      241 ggacaaatac cattttcaaa ctgtaaggtt acaaacggta gaaaagcaat ccgtagtatg
      301 tttgacctta aagtattcag tgaaatggta tatagataag gtagcgttta tgccgcgaaa
      361 gtcccttgac aatgagtatg cggcatgtta ggctacttag ccaggcatca tccatacttc
      421 aaagccccac tacctgttca gaatgggtgg tgccggtgtt gcctgaatac atgccgatag
      481 aggctcattg tcaagggaac cgtggaataa atgcggtaaa taggctcaaa ctaggcgcga
      541 aaatattggc ataatctcgt tacatggaaa aatgaaaaat ttgcccacga gtacttgaca
      601 caaacagttt gaaaaaaagt acttgcattg tttttggcga tgtagtaaaa tatcacttgc
      661 tgtgtgacac gacaaacgat gaaatgggag attgctattc tcagaaatag ggaacttcca
      721 tggagaatgt ccgattcaag aaaccgggcg acaagtcact gtacattttg cgacagtaca
      781 gaaagtgtgt ctgtttgtaa agtacagccc aggttttgca ggtcttagga ccgtgtaaac
      841 agcctgggtt ttaaaaatag aaaaacagga aacacccggc atagtgtaca gaataaaatg
      901 cttgttgtac gtaacattcg aggggatcag gagtcagaaa gcacgcggcg accgcagctt
      961 tcagaaacag cgggcagttc cgggaaggca ggtccggggc tgaggctctg aaaggcaaat
     1021 ccccgaacac tatcaaggag gttattgtat ggcaaacaat cagaaaatca gaatcaggct
     1081 gaaggcattc gatcaccaga tactcgatca gtcagccgaa aaaatcgtcg acactgccaa
     1141 aaggaccggc gcacaggttt ccggaccggt accgcttccg acaaagaagg aggtcttcac
     1201 catactgagg gctcctcaca agtataagga ttcgcgcgag cagttcgaaa tgaggactca
     1261 taagaggctg atagatattc tgatgcccac gcctaagacg gttgacgccc ttatgaggct
     1321 cgatctgccg gcaggtgttg atatagagat aaagctctga gctttatcgg gggagatcat
     1381 gttcgtgaga aatcatgaac caatgatgat taggaggtag caggatgagt aagtttattc
     1441 tcggtaaaaa aataggcatg acacaagttt ttgatgagaa cggcatcgta ataccggtaa
     1501 ctgttataca tgcaggcccg tgtgccgtcg tacagaagaa aaccgtcgaa aatgacggtt
     1561 atacggctat taagctggga tttgaagatg ttccggagaa gaaactgaac aagccggaaa
     1621 aaggcctgtt ttcaaaaatc aatgtcacgc ccaagaaata cctcagggag ttcaggactg
     1681 aggacatcga caagttcgaa gtcggacagc agatcaatgt tgctgacatg ttcagcagcg
     1741 gtgacaggat agatgtaagc ggtatatcca agggcaaagg cttccagggt gtcatgaaga
     1801 ggttcggttc ctccagaggt cccgaaaccc acggttcgaa gtatcacagg agagtcggta
     1861 ccatgggcgc cggcacaagc ccgggaagag tattcaaggg caagaaactg ccagggcata
     1921 tgggattgga aaaggtaacg gtccagaacc ttgacgtggt aaaggttgac agcgagagga
     1981 accttatgct tgtaaagggc gcagttcccg gtcctaaggg cggccttgtc atcgtacggg
     2041 atactgttaa ggccggcaag taatacagat gggaggagga agcgaaatgc caaaggttga
     2101 tttatacaac atgaaaggcg agacggtagg cgaaatagaa ctcagcgacg ccatattcgg
     2161 tatagatgtc aatgagaacg caatgtatct cgctgtacag aaccagttag caaacaggag
     2221 gcagggcaca cagtccacca agaccaagag tgaggtcagc ggcggcggca ggaagccgta
     2281 caggcagaaa ggcacaggcc gtgcacgtca gggaagcata cgctcggctc agtggataaa
     2341 gggcggtatc gtacatggac ctaaacccag gagctattcg tacactctgc cgaagaagct
     2401 caagaggctg gcactgaagt ctgcgcttac ttcaaaggtc aacagcaacg atatcctggt
     2461 gctggatcaa ctgaacttcg attccataaa aacaaagcag atggtaaacg tgctcgatgc
     2521 actcaaagtt gataaaggcg ccctgatagt acttgacagc aagaacgaga ccgttgtgaa
     2581 atccgcaagg aacatacctg gcgtcaagac ggcactggta aacacgataa acgtattcga
     2641 catactgaaa tatgacaaat tcataataac gaaggatgca gttgcaaaag tcgaggaggt
     2701 gtatgcgtaa tgagagcacc ggaagatgtg atcatcagac ctttcataac agagaaaagc
     2761 aaccaggaag tagcagccgg gaaatacacc tttatcgtag atgtcaatgc taccaagacg
     2821 gaaataaggc aagctgctga aaagcttttt caggtaaagg ttcttgaggt caataccgta
     2881 aattacagcg gcaaggaaaa gaggctgggc gttcatgtcg gaatcagacc cagttggaag
     2941 aaagccgtca tcaagataga cctcaacccc gaacctgaaa aatatctcac aaaaggcggc
     3001 aaagaagcag ttagtccgaa gaaatacaag accagcattg aagagtacgg tgtgacgcaa
     3061 taaagactcg gcttgagtgc ggcaaggtcc gtaaggcggc tgcatcacag cactggaacg
     3121 cagcttcggg aagagcggca acgagtccgg ccggatctgc gggaacaaca gagtaattac
     3181 gttctttgat taaggagtga gaagaaatgg cagtaaaaaa gtatagtcct acttctcccg
     3241 ccagaaggtt catgacagtt tcgactttcg aggagatcac gaagaaggaa cctgagaagt
     3301 cactggtaga gatactgaga aagactggcg gcagaaatgt ttacggcagg ataaccgtac
     3361 gccatatagg cggaggtatg aaaagaaaat acaggatcat cgatttcaag agagacaagg
     3421 acggcataaa cgctaaagtc gccgcaatag agtatgatcc caacaggact gcaaatattg
     3481 cacttctcca ctatacagac ggagaaaaga gatatattat cgcaccggtc ggactgaatg
     3541 tcggagatat cgtcgaatcc ggaccggatg cagacatcaa gccgggcaat gcgcttccgc
     3601 tggcaaatat acctgttggt acgatggtac acaacatcga actgaagccc gagaagggcg
     3661 gccagctcgt aagatctgca gggggtgcgg cccagctcat ggcaaaggaa ggcgattatg
     3721 cacagctcag gctcccttca ggcgaagtca ggatggtaag cctgcagtgc aaggcgacga
     3781 ttggccaggt cagcaatatc gagcacgaga acatttcgat aggcaaggcc ggaagaaaga
     3841 gatggatggg tgtaaggccg accgtacgcg gtgttgtaat gaacccggtt gaccacccgc
     3901 atggcggcgg tgaaggcaag tcaccgatag gaagaccaag cccggtcacc ccgtggggca
     3961 aacctacact tggctacaag accaggaaga agaacaagaa gtctgataag ttcatcgtca
     4021 agagaaggaa cgctaaataa tacactgcgg gattccatag ctggaaagga ggattaacac
     4081 cgaatgagca gatctttgaa gaagggaccg tttgttgatc agaaactcct taagaaaata
     4141 gaggagatga acagggccaa cgacaagaag gtcataaaga cctggtccag ggcgtcaacg
     4201 atattccctc agatggtagg
//
LOCUS       DAOSCC010000210         4198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13332_length_4198_cov_4.888245, whole genome shotgun sequence.
ACCESSION   DAOSCC010000210 DAOSCC010000000
VERSION     DAOSCC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13332_length_4198_cov_4.888245"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLG72_09860"
     CDS             complement(<1..370)
                     /locus_tag="PLG72_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ81141.1"
                     /translation="MMQQYLDIKEQYKDCILMFRLGDFYEMFFDDAVVASRELEIALT
                     GRDCGLEERAPMCGVPYHAIDSYIARLVQRGYKVAICEQMEDPALAKGIVKREVIRVV
                     TPGTVTDMAMLDEKKNNYLVS"
     gene            complement(565..1914)
                     /gene="miaB"
                     /locus_tag="PLG72_09865"
     CDS             complement(565..1914)
                     /gene="miaB"
                     /locus_tag="PLG72_09865"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ81142.1"
                     /translation="MHSVRELDPRANKKYMIHTFGCQMNENDSEKLAGMLESMGYRRT
                     DELEDSDLILFNTCCVRENAELKVYGHLGTLKPLKARKPGLIIAVCGCMMQQEEVVEH
                     ILDKYNHVDLIFGTHNLHKFPELLYRAVTSDTTVTDISSCDGYIAEGLPIRREHGVKA
                     WLTIMYGCNNFCSYCIVPYVRGRERSRRMSDILDEARLLGRQGYKEITLLGQNVNSYG
                     LDRKDGSSFAVLLRSLEEVDGIERIRFMTSHPKDLSDELIHAMRDCSKVCEHLHLPVQ
                     AGSNDILAAMNRKYTREHYFELVERVREQIPGISITTDIIVGFPGETDEDFGQTLDLV
                     ERIRFDYAYTFLYSRRTGTPAAKKAEQVSEEVKKKRFDELLKLQNSISSEINERLEGT
                     AAEILVEGPSKNNIRMMTGRTRTNKIVNFSGNRDLAGKLVNVRIDKTGTWSLDGTLV"
     gene            complement(2005..3369)
                     /gene="nagZ"
                     /locus_tag="PLG72_09870"
     CDS             complement(2005..3369)
                     /gene="nagZ"
                     /locus_tag="PLG72_09870"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ81143.1"
                     /translation="MKTKTVLTIAVFVFFALILMMVPGCSAVGKKPSDPSENGTGIPG
                     GDPSVRDGSGGENGSRGRTDAETGDQVENDGNIQPGEGSGTTETGSFEERLATMSLDE
                     KIGQMFIIGVEGTSVDKDMLDILAALKPGGIILFRKNITDPYQLVTFTNSLKIANPAQ
                     APLFISIDEEGGRVSRMPGHISGIPPALSVGELDDESFTYETGRLIAEMVKSFGINMD
                     FAPVLDIFSNPDNTVIGDRAYGTTPLSVVEHGIQVMKGIRDEGIISVVKHFPGHGDTD
                     VDSHYGLPAVVHDKGRLSNFELVPFRKAIEENADAIMVAHIVVESVDPGLPASLSDAV
                     INGLLRGEMGFEGVVVTDDMTMGAITEYFDIGDAAVKSVLAGNDIILVCHGYENQKKA
                     MDAIKDAVGSGIINEGRIDESVARILTLKKKYSLNDRSVRYPDVERLNKKIEGVLSKW
                     YNDN"
     gene            complement(3469..>4198)
                     /locus_tag="PLG72_09875"
     CDS             complement(3469..>4198)
                     /locus_tag="PLG72_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ81144.1"
                     /translation="LYGSESSALELLDTIDGLKDDRLRCPERLLAVLEDTYTGNTVYK
                     GVIDSLLDFIGDNIDTDKIDYISGGERRDWFFSLLAAKRLGKPHITIFKDLSAFITEN
                     GKTSEAESISGARVLHIADLITEASSYERAWIPAIKNIRGQMTCTVVIVDRMQGGSEL
                     LEKYGVCSFAMVSINRNFFDKALEMGKINSAQYDLLLKYASDPHGSMKAFLEEHPEFI
                     ENALKSDEKTRERARMSIEKGIYR"
BASE COUNT          905 a         1164 c          895 g         1234 t
ORIGIN      
        1 tcgatacgag atagttgttc ttcttttcat ccagcatcgc catatccgtg acggtgccgg
       61 gggtcaccac ccttatgact tcacgcttta cgataccttt agccagcgcc ggatcttcca
      121 tctgctcgca gatggccacc ttgtaccccc tctgtacaag cctcgcgata tagctgtcta
      181 tggcgtggta aggcactccg cacatcggag ccctctcttc aagaccgcag tcccttcctg
      241 tcagcgctat ttccagttcc ctggacgcga ccacggcatc atcgaaaaac atctcataaa
      301 agtcgccgag cctgaacata agtatgcagt ctttgtactg ctcttttatg tccaggtatt
      361 gctgcatcat cggggtaaca gtcaaaggta tcgacctcac ttacgtttga atcgcttttg
      421 cttctattta ttacttctgt tgacttcttt tgaacggcct ttgatttttt gctcggtaca
      481 acaagccgcc actgcttttt tcaaggttca ggtccgctca cgccccggtc ggactttaga
      541 gaaaaccggg gatcagacct gagatcacac cagggtgccg tccagagacc acgtaccggt
      601 tttgtctatc cttacattga ccagttttcc ggccagatcg cggtttccgc tgaaattgac
      661 aatcttgttt gtccttgtgc gcccggtcat catcctgatg ttgtttttgc tggggccttc
      721 caccagtatc tcggcagcgg tcccttccag cctttcgttt atttcgctgc tgatgctgtt
      781 ctggagcttc agcagttcat cgaacctctt cttctttact tcttcgctga cctgttcggc
      841 tttcttcgca gcaggagtac cggtccttct ggaataaagg aaggtatacg cataatcgaa
      901 cctgatcctt tcaacaaggt ccagcgtctg cccgaagtcc tcgtccgtct ctcccgggaa
      961 tccgacgatt atgtctgtgg tgatcgatat gccgggtatc tgttccctga ccctttccac
     1021 aagttcaaaa tagtgctcac gggtatattt ccggttcatt gcagcgagta tgtcgttgct
     1081 gcctgcctga acaggcagat gcaggtgctc gcataccttg ctgcagtctc tcatggcatg
     1141 gatcagttca tcagaaagat ccttgggatg ggaagtcata aaacggatcc tttcgatccc
     1201 gtcaacttcc tcaaggctcc gaagcagcac tgcgaaactg cttccgtcct tcctgtcgag
     1261 cccgtatgag ttcacattct gtcccaggag tgtgatctcc ttgtaaccct gtcggccaag
     1321 gagccttgct tcgtcaagga tgtccgacat cctgcggctc ctctcgcggc ccctgacata
     1381 cggaactatg cagtaggaac agaaattgtt gcagccgtac attatcgtga gccatgcctt
     1441 tacgccatgt tcgcgcctga taggaagccc ttcggcgata tagccgtcgc atgagcttat
     1501 atcagtaact gtcgtgtcac tggtaacggc cctgtataaa agctcaggaa acttgtgcag
     1561 attatgggtc ccaaatataa gatccacatg gttgtattta tcaaggatat gttcaacgac
     1621 ttcttcctgc tgcatcatac agccgcatac agctattatc agcccgggct tccgggcttt
     1681 cagcggcttg agcgtgccca ggtggccata cacctttagt tccgcatttt ctctcacgca
     1741 gcatgtgttg aaaaggataa ggtcggaatc ctcaagctca tccgtacgcc tgtatcccat
     1801 ggattccagc atccctgcca gtttttccga atcgttctcg ttcatctggc atccgaaagt
     1861 gtggatcatg tattttttat tggccctggg atccagttcc cttacggaat gcatgaacgt
     1921 ctcctgcctg gttatttcat cttcagaaac tatgtttctt tctctgtcag ccaattctta
     1981 cctcctgcga tcattacaaa ggaactagtt atcattatac catttcgaca ataccccttc
     2041 aattttctta ttaagcctct ccacatccgg atacctgaca gatctgtcat tcaaagagta
     2101 ttttttctta agtgtcagta tccttgctac gctctcgtcg atcctaccct catttatgat
     2161 accggatccg acagcatcct ttattgcgtc cattgccttc ttctggtttt cataaccgtg
     2221 gcagacaagt atgatatcgt tgcctgcaag gaccgacttt actgccgcgt ctccaatatc
     2281 gaaatattcc gttattgccc ccatggtcat atcgtctgtt accactacgc cttcaaatcc
     2341 catctcaccc ctgagcagcc cgttgataac ggcgtccgaa agtgaagccg gcaaccccgg
     2401 atcgacagat tccactacta tgtgcgctac cattattgca tcagcatttt cctctatagc
     2461 ctttctgaat ggtacaagtt caaaattact gagcctgccc ttatcatgaa caaccgccgg
     2521 gagaccatag tgtgaatcga cgtctgtatc tccatgaccc ggaaaatgct tcactacgga
     2581 aatgatccct tcatcgcgta tgcctttcat cacctgtatc ccgtgctcta ctaccgacag
     2641 gggggtcgtt ccgtatgccc tgtcacctat tacggtatta tcgggattgc tgaagatatc
     2701 gagaacggga gcaaaatcca tatttatgcc gaatgatttt accatttccg caatcagcct
     2761 gccggtttca tatgtaaaag attcatcatc cagttcgccg acagacaggg ccggcggtat
     2821 gccagagata tgcccaggca tcctgctcac ccttcctcct tcttcgtcaa tggagatgaa
     2881 aagaggtgcc tgggcaggat ttgcgatttt aaggctgttt gtgaatgtaa cgagttgata
     2941 cggatcggtt atatttttcc tgaaaagaat gatgcccccg ggtttcaggg ccgccagaat
     3001 gtccagcatg tccttgtcga cggaagtgcc ttctacgcct atgatgaaca tctgtccaat
     3061 tttttcgtca aggctcatcg ttgcaagtct ttcctcgaag ctgcctgtct cggtcgtacc
     3121 actgccttct ccgggttgaa tgtttccgtc attttcgacc tgatcaccgg tttcagcgtc
     3181 ggttcttccc cggctgccat tttcgccgcc ggaaccgtct ctgactgacg gatctccacc
     3241 aggaatacct gttccgtttt ctgaggggtc cgaaggtttt ttgccgactg cagaacagcc
     3301 tggcaccatc atcaggatca aagcaaaaaa aacaaaaacc gctatagtca aaacagtctt
     3361 cgttttcata tcctgccacc tttcatgcgt catctgttgc tgcttcttaa tcctgtcttc
     3421 aatgattctg ctgtgacagt tccatcagcg caggtattga tctgcgtttc acctgtatat
     3481 ccctttctca atgctcatcc gtgccctttc cctcgtcttt tcatccgatt tcagagcgtt
     3541 ttcgatgaac tccggatgct cttccaggaa cgctttcatg gatccatgcg gatcagaagc
     3601 gtatttcagg agcaaatcat actgggcgct atttatttta cccatttcaa gcgccttgtc
     3661 aaaaaagttc ctgtttatgg ataccatggc aaatgagcat acaccgtact tctcaagcaa
     3721 ttcagaccct ccctgcatcc tgtcgacgat cacaacggta catgtcatct ggcctcttat
     3781 attttttatt gccggtatcc atgccctttc atagcttgat gcttcggtga tcagatcggc
     3841 gatatggagc accctggcgc cgctgatgga ttctgcttcg cttgtcttgc cattctcagt
     3901 gatgaacgcc gacagatctt tgaatatagt aatgtggggc ttgccgagtc tttttgctgc
     3961 aagcagcgaa aaaaaccagt cccttctttc tccgccggaa atatagtcga ttttatccgt
     4021 atctatgttg tcccctatga agtcgagcaa gctgtcaatg acgcccttat atacagtatt
     4081 gccggtatat gtatcctcca ggactgccag cagcctttcg gggcaccgca gcctgtcatc
     4141 cttcaggcca tctatcgtat ccagcagttc cagcgcgctg ctttcggaac cataaagg
//
LOCUS       DAOSCC010000211         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13341_length_4195_cov_4.634300, whole genome shotgun sequence.
ACCESSION   DAOSCC010000211 DAOSCC010000000
VERSION     DAOSCC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13341_length_4195_cov_4.634300"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..641)
                     /locus_tag="PLG72_09880"
     CDS             complement(150..641)
                     /locus_tag="PLG72_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HOJ81145.1"
                     /translation="MRIESKEAFNVIGIKTWIPETDNNAFGEFWERCHKEGDAERIKK
                     FNKNKENSCTKSVILGLSCTEKDPYNRSFYFYIAAETDEISDQGKYEVYKVAPYEWAI
                     FSSNGSDINALMECEMYAWKEWLPDNDLYEHDNGPEMEVYFDDNKIEYWLPIRRKRIN
                     DFR"
     gene            860..1570
                     /gene="sigK"
                     /locus_tag="PLG72_09885"
     CDS             860..1570
                     /gene="sigK"
                     /locus_tag="PLG72_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOJ81146.1"
                     /translation="MLHFLYTILSEVLGSIVFQLGYVTNANSFPQPLTPEEEQKYIAA
                     YKEGSEDARNILIERNLRLVAHIVKKYSTFSSDIDDLISIGTIGLIKAISTFDQSKGT
                     RLATYAARCIENEILMQIRSTKKIQSEVSLHDPIGVDREGNEITLIDVIGNETESVID
                     EVELKLQVKRLYNKMKSVLKKREKTVLELRYGLLNGTSKTQREIAGMLGISRSYVSRI
                     EKKAIKKLSKELEPESGH"
     gene            complement(1823..2248)
                     /locus_tag="PLG72_09890"
     CDS             complement(1823..2248)
                     /locus_tag="PLG72_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOJ81147.1"
                     /translation="MKIAVVDGQGGGIGKVIVERLRSRYNDSVDILALGTNALATSAM
                     LKAGADEGATGENAIVYNADKADVIVGVIGILAANSMLGELSPAMAAAIAGSPALKVL
                     IPVNKCCIQVAGIKDMTLLQFIDEAIDMIDRHMGCRDAC"
     gene            complement(2415..3587)
                     /locus_tag="PLG72_09895"
     CDS             complement(2415..3587)
                     /locus_tag="PLG72_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ81148.1"
                     /translation="MQLNTLFYLALILFAGLLFGRVVKLAKLPNVTGYLIAGLLIGPF
                     VLRLVPADTVKGLELVSEMALAFIAFSIGSEFKITYLKKVGPMPVVIAIFEGLTATSV
                     VALILALSGFDAELALLLGAIASATAPAATIMVIKQYRAKGPVTETLLSVVALDDAVA
                     LIAFGFAVAIVNMMQNPGETSLILSILKPVGEILGSLLLGFLLGIMFKIPLRYFKKDS
                     NRLIITSGFVFLGSSLASMLGLSPLLLCMAMSATLVNVSSEGISILKLADSVTPPIFL
                     MFFVVSGMELDITVLPQVGLIGILYIIFRVVGKIVGAAIGAAIMKAPAVIRKYVGFSL
                     VPQAGVAIGLSLLAAQLVPEYAQTIRAVVLSATLIYELTGPAITKVALNKAGELNA"
     gene            complement(3590..3931)
                     /locus_tag="PLG72_09900"
     CDS             complement(3590..3931)
                     /locus_tag="PLG72_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81149.1"
                     /translation="MQLMVMVLNRVEVLDTLLEKLMEDGICGATILNSTGMVRELAKS
                     GEDFPIFGGSLRMLIDPERKESKTLFMALTDEKVEQAKKIIREVVGDLSKPDTGVIFT
                     LPVLSTEGVEF"
     gene            complement(4041..>4195)
                     /locus_tag="PLG72_09905"
     CDS             complement(4041..>4195)
                     /locus_tag="PLG72_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ81150.1"
                     /translation="GVVHAWFTGYFPAAEPRYAVAVFAEDGRLGGKSAAPVFAEIAKG
                     MLEKGY"
BASE COUNT         1104 a         1112 c          886 g         1093 t
ORIGIN      
        1 ctattgttct gccagattat ccctttaaat tccctacagt aactttacgc taacctgtca
       61 tattcccgga tcaatgagcg agatccgcag aacaccgcca attccaacag gcagccggtc
      121 tgaacataac atggcgacga aagcgccatt taccggaagt cgtttattcg cttccttctt
      181 attggcaacc agtattctat tttattatca tcgaagtata cttccatttc cggaccgtta
      241 tcatgttcat acaaatcatt atcaggcagc cattctttcc aggcatacat ctcgcattcc
      301 atcaatgcat tgatatcact gccgttgctt gagaatattg cccattcgta tggggcgact
      361 ttataaactt catatttccc ctggtctgat atttcatcgg tttctgcagc aatataaaag
      421 taaaagctcc tgttatatgg atctttttcc gtacaggaca atcccagtat tacgcttttg
      481 gtacaactgt tttccttatt tttattaaac ttctttatcc tttctgcatc gccttccttg
      541 tgacaccttt cccaaaactc tccgaacgcg ttgttgtcgg tttcaggtat ccatgtctta
      601 atgccgatta cattgaatgc ttctttgctt tctatacgca ccgaaaccaa gtttagccct
      661 ccattccgtt cctgttgttt tattgttccg cccacaatat tgaatttctg ccggtgtcag
      721 ttgacattat catattggcc gtttgttttc aatatttttt agtaaatgtc tcagaaaata
      781 attattttct gtacaagcaa tttacttcga tatgcctcat atacttatac ggtgactaat
      841 tttccgggag gtgcctcatt tgctgcactt cttgtacacc atcctcagcg aggtactggg
      901 cagcatagtg ttccagcttg gatatgttac caatgccaac tcgttcccgc agcctcttac
      961 tccggaagaa gaacagaaat acatcgcagc atacaaggaa ggcagcgaag atgccaggaa
     1021 tatactgata gagaggaatc tgcggctcgt ggcccatatc gtgaagaagt acagcacgtt
     1081 cagcagcgac atcgacgacc tcatttccat tggcaccatt gggctgatca aggccatctc
     1141 cacgttcgac cagtccaaag gtacccggct tgcgacctat gctgcccgct gcatagagaa
     1201 tgaaatattg atgcagatca gatcaaccaa gaaaatacag agcgaggtat ctctccatga
     1261 cccgatcgga gtggatcggg aaggcaacga aattaccctg atcgacgtta ttggcaacga
     1321 aaccgaatcc gtgatcgacg aagtggaatt gaaactccag gtcaagcgtc tttacaataa
     1381 aatgaaatca gtgctcaaga aacgggaaaa aaccgtattg gaacttcgtt acggcctact
     1441 gaacggaaca agcaaaacgc agcgtgaaat agccggcatg ctcggtattt cgcgatccta
     1501 cgtttcgcgt atagagaaaa aagctatcaa gaagctcagc aaggaacttg aaccggaaag
     1561 cggtcactga cgggggcgaa atgcccccgc ggcctctcct taaggatagg acgggcactt
     1621 cgaacaatac atctgcggaa catcagatgt gatcatcaga tgtgatcatc agatgattca
     1681 gccgtaaaac accgaataca gtgatacgtt tctgtaccgg atacagcggc aaggtttctg
     1741 tatcggccat taaggcgtat atgcaatcct ttacttgggc tgaattccgg cgttgtaata
     1801 tataagtcca catcccaata catcagcagg cgtcccggca tcccatgtgc cggtctatca
     1861 tgtcgatcgc ctcatcgatg aactggagaa gggtcatatc ctttatccct gccacctgta
     1921 tgcagcattt gttcacaggt attagtacct tcagcgccgg gctcccggca atggctgccg
     1981 ccatggccgg cgaaagctcc cccagcatcg agttggctgc gagtatccct attacaccga
     2041 cgatgacatc cgccttgtcc gcattgtaaa ctatggcgtt ttcaccggta gccccttcat
     2101 cggctccagc cttcagcatt gccgaagtgg caagcgcatt ggtgccaagg gccagtatgt
     2161 caacggaatc attgtaccgg ctcctcagcc tttcaactat aactttccct attccgcctc
     2221 cctgtccgtc cacgaccgca attttcataa aacactatga cctcctggtt actggttttt
     2281 tgtatgtcag caccaactgc cgccaaaatg atgccgtccc gcaaaacatc cgtgagctgt
     2341 aataaacaat atatgtaagc catgatatgt aatgtgatac atgtcttcag ttccggccat
     2401 gcatgatatt ccagtcatgc attcagttct cctgctttgt tcagggcaac ctttgtgatc
     2461 gccggacccg tcagttcata tatcaaagtc gcgctcaaca ccacagcgcg tatggtctgg
     2521 gcatactccg gcacgagctg cgccgccagc aatgagagtc cgatcgccac tcccgcctgc
     2581 gggacaagtg aaaatcccac atactttctg atcactgcgg gagctttcat tatagcagcg
     2641 ccgatcgctg cccccactat cttcccgacg acacggaaaa taatataaag tataccgatc
     2701 aatcctacct gcggcagaac ggtaatatca agttccatac cagaaaccac aaaaaacatg
     2761 aggaatatcg gcggtgtcac ggagtctgcc agtttcagta tcgagatccc ttcactgctc
     2821 acattcacaa gcgtagcgct catggccata caaagaagaa gcggtgacaa gcccagcatc
     2881 gacgcaagcg aactgccgag gaaaacaaat ccgctcgtga tgataaggcg gtttgaatct
     2941 tttttgaaat accgcagagg gatcttgaac atgatcccga gcaaaaaccc aagaagcagc
     3001 gatcccagga tctcgccgac aggcttgagt atcgacagga taagtgacgt ttctcccggg
     3061 ttttgcatca tgttgactat tgcgactgcg aacccgaatg ctataagtgc aacagcgtca
     3121 tcaagcgcca caacgctgag cagagtttca gtgacaggtc cctttgccct gtattgcttt
     3181 attaccataa tggtcgccgc cggggccgta gcagatgcaa tggcgccgag cagaagcgca
     3241 agctcagcat caaagcctga caatgcgagg atcagcgcaa ctacggaagt tgctgtcagt
     3301 ccctcgaaga tagcgatcac gacaggcatt ggacccactt ttttcaaata tgtaatcttg
     3361 aattccgagc ctatggaaaa tgctatgaat gccagcgcca tctcggaaac aagttccagg
     3421 ccttttaccg tatccgcagg caccagcctg agcacgaatg gaccgatcag aaggccggca
     3481 atcagatatc ctgtgacatt gggaagcttt gccagtttta cgacacggcc gaacaaaagc
     3541 cctgcaaaaa ggatcagcgc caggtaaaaa agtgtattca gctgcatttt cagaattcaa
     3601 ctccttctgt gctcaggaca ggcaatgtga atattacccc ggtatcaggc ttcgaaaggt
     3661 ctccgacgac ttcccgtatt atctttttag cctgctcgac tttttcatcc gtcagtgcca
     3721 taaacagcgt cttgctttcc ttcctctccg gatctatcag catacgcaga gatcctccaa
     3781 agatagggaa atcctcgccg ctttttgcaa gttccctgac cataccggtg ctgttcagga
     3841 tagtcgcacc gcatatcccg tcttccatta gtttctcaag cagtgtgtcg agtacctcta
     3901 ccctgttcag taccatcacc ataagttgca tttatacatc acctcaaata aaaaggtcat
     3961 acttaaaaca acagctttaa gtataacctt tcatttagcc aagtcaaccc attggattta
     4021 tttttatgtt gcctgcagca ttagtatccc ttctcaagca tacctttggc gatctcagca
     4081 aatactggtg ctgctgattt gcctccaagc cggccgtctt cagcaaatac cgctactgcg
     4141 tatctgggtt ccgctgccgg gaaatatccc gtgaaccagg catgtacgac acccg
//
LOCUS       DAOSCC010000212         4164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13476_length_4164_cov_3.427841, whole genome shotgun sequence.
ACCESSION   DAOSCC010000212 DAOSCC010000000
VERSION     DAOSCC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_4164_cov_3.427841"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..510
                     /locus_tag="PLG72_09910"
     CDS             124..510
                     /locus_tag="PLG72_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ81151.1"
                     /translation="MKRGRPGRILKMAVRQVFCKPATILYPKGKMQIADKYRGRLVYD
                     PSNCVGCNLCMRYCPAGALKIVNDGTKENRKMKAYLNVGHCIFCCQCVDSCPKKCLSF
                     SQDIDLSSFRKEDLTVCLNEGIDEDK"
     gene            497..991
                     /locus_tag="PLG72_09915"
     CDS             497..991
                     /locus_tag="PLG72_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HOJ81152.1"
                     /translation="MKISDYLKSKIIPGRKYAVLGIGSTQRSDDAAGMYFTELLSGMI
                     KQDNVLLIAGSTAPENFTGVIRDFGPDILFIVDAAYMGFLPGEVKIVPACDIGGVSFS
                     THILPLTVMLDYLQAELGCEVLFIGIQPASTDYGFYMSDDVRRGVERLAEEFCSALQL
                     SGIF"
     gene            1185..2861
                     /locus_tag="PLG72_09920"
     CDS             1185..2861
                     /locus_tag="PLG72_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOJ81153.1"
                     /translation="MIRRPGREKPKKRSRYSEKPDKTAVTGESAEPAADSDISPSGNA
                     RTNRAIENEKSTMHGNSARSQSDKQSETEQGLTSANVEGLLQYSSDIVFRRLHINGRK
                     DLPVTMVLIDGVIDTKAVDDYILKPLIQEGSLSECSSTSEIIEKIEHGVIYHAAAKIR
                     RDFSECVNDIIDGSVALIFDDDKTAVTFDIKGFRERSISEPTGENVIKGAKDSFVENL
                     RTNTASIRIKIKNPRLVIEETKIGKQTATRIAIVYIKGLTNAHIVEELKKRLNKLDTD
                     AVLTTGVIEEAIVDRKTSPFPQVITTERPDKFCSTIVEGRVGLIIDGIPVTIVVPGTF
                     GAFLQAPEDYSQSFIVNSIIRSMRYALMLVTLFLPGFYISVSSFHIEMIPTDLALAIT
                     ASKEGVPFLTFIEVIFMLLAFEVLVEAGLRLPKNIGQAVSVVGAVVVGQAAVEARLVS
                     PAVVVIIAVTAISSFTMPNQDFSNALRLWRFIFAILSSIIGLYGLTIGAIILLNHLSS
                     IEVFGVPYLSPFVGGEGKNLQDAIFRFPFSSMRKRPMNLRTTNKKRRGAE"
     gene            2858..4054
                     /locus_tag="PLG72_09925"
     CDS             2858..4054
                     /locus_tag="PLG72_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOJ81154.1"
                     /translation="MRYVKMIVLLCCFICCPVVLTGCRIAFWDTTQINNIEFIRAVGV
                     DKADNGNIRFTIATQRIISGGARGGSEQKQSEIVYSEGRTSFEAMRNFWSYSEKRPFL
                     GHLEFILIGEEAAKEGILKYLDFFTRDAEVRLNLNVFVVKGNDAGEVIRQGNAQDKFV
                     FDRLEGIKRHDWGLSIINDVNLMEIMYILDAKYLSLYIPCVRLLNRAETTEESRDGME
                     IALEGFAVFDGDKVAGYLDKEMARGLNFLKNKLKSGVVNVRSKNGTMVALEIISGGSK
                     MKPYIKDGELHIAVEVEMDSSIAEIQGPENIFSDDSLEYLEKQQEQAIMDEVRKTIDF
                     AQEKKLDFFGAGDAVLHKYPYEWEDFYEKDWRDVFSQVEFEITARSKISRTYDIKQPI
                     GAKDIR"
BASE COUNT         1208 a          853 c         1127 g          976 t
ORIGIN      
        1 gatgcgcgct gcaatggcac ggctcagggt agaccagatg gtgaactttt gctggcggtt
       61 attgacgcct gcggcgatcg tacagatcat cctggatctt gtgataaggg gttggttggt
      121 catatgaaga ggggaagacc cggcaggata ctgaaaatgg ccgtcaggca agttttttgc
      181 aagcctgcga cgatactata tccgaaaggc aaaatgcaga tcgcagataa ataccgggga
      241 aggctcgtgt acgacccttc aaactgcgta ggctgcaacc tgtgtatgcg ttattgcccc
      301 gcaggcgcgc tgaaaatcgt aaatgatgga acgaaggaaa acaggaaaat gaaggcatac
      361 ctgaatgtcg ggcattgtat attctgctgc cagtgtgttg attcctgccc caaaaagtgt
      421 ttatcattca gccaggatat cgacctttca agctttagaa aagaggatct gacggtgtgt
      481 cttaatgaag gcatcgatga agataagtga ttatctgaaa agtaaaataa tacccggccg
      541 gaaatatgcc gttcttggga tagggtcgac gcagaggtcc gacgatgccg cggggatgta
      601 ttttacagaa ctcctcagcg ggatgataaa gcaggacaat gtgcttttga tcgccggttc
      661 gactgctcct gaaaatttta ccggggtgat aagggatttc ggaccggaca tacttttcat
      721 tgtggatgcg gcatatatgg gatttttgcc aggagaggtc aaaatagtac ctgcctgcga
      781 tataggcggc gtttcttttt cgacgcacat actgccgctg accgtgatgc tggattacct
      841 ccaggccgaa ttgggctgtg aagtgctgtt cattgggata cagccggcaa gtactgatta
      901 tggcttttac atgagcgatg atgtgaggag aggggtagag cggctcgccg aagaattctg
      961 ttccgcactg caactttccg gcatttttta ggcaatagca tcaacctcag aaaaacttgc
     1021 tatcgtggtt ggttttgaac atagggacgg ttctcatgtc tgagtcctcc gggaaccatg
     1081 catgagaacc gtcccatgtc ttttcttgag gataaatgcc aggcgtcttc tgaatgtctc
     1141 agggatgaaa tgcaaataat atcacatacg atgggtgtga ttgcatgatc agacgacctg
     1201 gaagagaaaa accgaaaaaa aggagcagat acagcgaaaa acccgacaag acagcagtga
     1261 cgggagagag cgcagagcct gcagcagaca gcgatatctc gccgtccggc aatgcccgga
     1321 caaatcgggc aattgaaaat gagaaaagta caatgcatgg taatagcgcc aggtcccaat
     1381 cagacaaaca gtccgaaaca gagcagggac ttacgtcagc caatgtcgag ggtttgctcc
     1441 aatacagcag tgatatcgta ttccgcaggc tccatataaa cgggcgtaaa gatcttcctg
     1501 tcaccatggt gctgatagac ggagtgatcg atacgaaggc cgtggatgac tacattctaa
     1561 agccgctgat acaggaaggg agcctcagcg aatgcagcag cacttccgag ataatcgaaa
     1621 agatcgaaca cggtgtgatc tatcatgctg ctgcgaaaat ccgccgggac ttctccgaat
     1681 gcgtgaatga tataatcgac gggtcggtgg ccctgatatt cgatgatgac aaaaccgccg
     1741 taactttcga catcaaggga ttccgtgaaa ggagcatatc ggaacccaca ggagaaaatg
     1801 taataaaggg cgcaaaggac tcctttgtgg aaaacctcag gacaaatacg gcttcgatca
     1861 ggataaagat caaaaacccc aggctcgtca tcgaagaaac gaagatagga aagcagactg
     1921 cgacaaggat cgctatcgtg tatatcaagg gactgacgaa tgcccacatt gtcgaagaac
     1981 tgaagaaaag gctcaataaa ctggatacag atgcggtcct gacgacgggg gtcatcgaag
     2041 aggcgatcgt cgaccgcaag acatcgcctt ttccgcaggt aataacaaca gaaagacctg
     2101 acaagttctg ttcaactatt gtcgaaggaa gggtaggcct catcattgac gggattcctg
     2161 tcacgatcgt agtgcccgga acattcggcg cattcctgca ggcaccggaa gattattcgc
     2221 agagtttcat cgtcaactcg ataatcaggt ccatgcgtta tgcgctgatg ctcgtaactt
     2281 tgtttttgcc cggattctat atttccgttt cgtcattcca tatcgagatg ataccgacag
     2341 accttgcgct ggccatcaca gcgtcgaagg aaggagtgcc gttcctgaca ttcatagaag
     2401 tcatattcat gctgctggcc ttcgaagtgc tggtggaagc aggattgagg ctgcctaaga
     2461 atataggcca ggcggtctcc gttgtcggag ccgtggtggt gggacaggcc gccgtggaag
     2521 caaggttggt ttcacctgct gtagttgtca taatagccgt tacagcgata tcgtcattta
     2581 caatgccaaa ccaggacttt tccaatgctc tgaggctgtg gagattcata ttcgccattt
     2641 tgagcagcat catcggacta tatggcctta ccatcggagc gattatattg ctgaaccacc
     2701 tttcgagcat agaagtgttt ggcgtgccgt acctgtcgcc ctttgtcgga ggggaaggga
     2761 agaatctgca ggatgccatt ttccgttttc cgttctcttc aatgcgcaaa cgtcccatga
     2821 atctcaggac aacaaacaag aaaaggagag gtgcggaatg agatacgtga aaatgatcgt
     2881 actgctttgc tgttttatat gctgcccggt cgtgctgaca gggtgcagga tagctttttg
     2941 ggataccacc cagataaata atatcgagtt catacgcgca gtcggtgtcg ataaagctga
     3001 caatggcaat ataagattta cgatagccac tcaaaggata atctccgggg gagcaagggg
     3061 aggatccgag caaaagcagt ctgaaatagt gtactctgaa gggagaacaa gttttgaagc
     3121 catgcgcaac ttctggagtt attctgaaaa aaggcctttt ctcggacacc tcgaatttat
     3181 ccttatcggc gaggaagcag ccaaagaagg gatcctgaaa tacctggatt ttttcacacg
     3241 ggatgcggaa gtccgtctca atttaaatgt gtttgtcgtg aagggaaatg atgccggcga
     3301 agtcatcaga cagggcaatg cacaggacaa gttcgtattt gacaggctgg aaggcataaa
     3361 gagacacgat tggggattgt ccataataaa tgatgttaat cttatggaga tcatgtacat
     3421 actggatgct aaataccttt ctttatatat accttgtgtc agactgctaa acagggcaga
     3481 aacgacggaa gaaagcagag atgggatgga aatagccctc gaaggctttg cggtgtttga
     3541 tggcgacaaa gtcgcaggct atctggacaa agaaatggcg agaggcctga attttctgaa
     3601 gaataaattg aaatccggcg tagtgaatgt cagaagcaaa aatggaacga tggtcgcact
     3661 ggaaatcata agcggcggca gtaaaatgaa accatatata aaagacggtg agctccatat
     3721 tgcggtagaa gtcgaaatgg acagcagcat tgcggaaata caggggcctg agaatatatt
     3781 cagcgatgat agcctggagt atctggagaa acagcaggaa caggccatca tggatgaagt
     3841 caggaagacc atcgatttcg cccaggagaa aaaactggat ttttttggcg cgggtgacgc
     3901 cgtactgcac aaatacccgt atgagtggga ggacttttat gaaaaggact ggagagatgt
     3961 attttcacag gtcgaatttg agataactgc caggtccaaa ataagcagaa cgtatgatat
     4021 caagcagccc ataggggcca aggatataag gtgacgatga tggtaatatt attagttgca
     4081 atagtggcag ctatcagtgt gtttgaaata atgcgcatgg caaagaaccg acagataaaa
     4141 gagattattg tgttcattgt gata
//
LOCUS       DAOSCC010000213         4108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13722_length_4108_cov_4.322230, whole genome shotgun sequence.
ACCESSION   DAOSCC010000213 DAOSCC010000000
VERSION     DAOSCC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13722_length_4108_cov_4.322230"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..274)
                     /locus_tag="PLG72_09930"
     CDS             complement(89..274)
                     /locus_tag="PLG72_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81155.1"
                     /translation="MMGRNFSRKHASDVETARETIIPFSSAAGVPKRTDRESRKMTVQ
                     ANMQQDRIFDDLADKRT"
     gene            370..1083
                     /locus_tag="PLG72_09935"
     CDS             370..1083
                     /locus_tag="PLG72_09935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ81156.1"
                     /translation="MKLDGRLGMIAENIPRCSILADVGTDHAFIPIYAVEASLCERAV
                     AADVRPGPLTAARKNIAKHGLESRIETRLGDGLGPICEPECDVVVIAGMGGPLIRRIL
                     AESSEKAKKCRLLLLQPNNAPEALRKWLYENCFSIIGEDLAEDSGKLYCLIKAKWTGT
                     YEKRDDFDCYIGRKLLESDDPLIIPYLKKKLGELDTIIDGRSKSDPGKPRRVEYDSYM
                     DTKTCMEIRDRLRAYLDAR"
     gene            1215..1982
                     /locus_tag="PLG72_09940"
     CDS             1215..1982
                     /locus_tag="PLG72_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ81157.1"
                     /translation="MITCGDVMDFLQELAPAELAEDWDNIGLMTGGRKRKVGRILVCL
                     DPTADALDKAAEIGADLLVTHHSLIFKGLKSVNDDDIVESRVYTAISKGISVISAHTN
                     LDYADSGVNVQLASVLGLCDLEVLGHGPGRVGMLKERTDLETFISHVKTSLGISHVRV
                     AGNAASGVRKVAVFGGSFDGDLDAVKRSCADVVVTGEMKHHTALDVREAGLCFIVASH
                     FSTERVVVPYLASAISGRFPSVEVICFDQEKDPFITY"
     gene            2029..2392
                     /gene="rnpB"
                     /locus_tag="PLG72_09945"
     ncRNA           2029..2392
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG72_09945"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2477..>4108)
                     /locus_tag="PLG72_09950"
     CDS             complement(2477..>4108)
                     /locus_tag="PLG72_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ81158.1"
                     /translation="IAESNLVKTRESIESSRAWFGRHEELDAILEVSPDSYSLSFAWL
                     IRSTKTAKSIGILVIDVNADVVSELFESQIIYEGQQFIMVTPDNRVVVNAVDETDTST
                     ITQQDFYKKLLGSDEAIGADTVSMDGKDFFMVFRKISRTGITLINLIPDSILEASANR
                     IIISTVLTCVAAVIIAFAAGILMANSMGRTINRIISASARAASGDLTVTLQSRRKDEL
                     GELTRSISSMIASMRNLIVQTSEVAEKVTGSAEIVSSTSQQVSNVSKDITRAIQEIAT
                     GAGAQAADAEQGVRKISILDQKINDVIKSAKLIDELTGDTKSLTQTGLATVEDLDVKA
                     GRTTDITREIIEDIKNLEAQSRSIGKIIKVISGIADQTNLLSLNAAIEAARAGEAGKG
                     FAVVADEVRKLAEMSMESAREIANIIKSTQDLTAKTADKANDTEAYLKSQNEAVQGTI
                     QIFNRIMGSMGNLSEQVERIMSGVAEMEENKTQAINSIQNISAVSQQTAASTQEVTAS
                     TEEQLSFIEELSRFADELKASSEELQQTIARFKLE"
BASE COUNT         1013 a          960 c         1079 g         1056 t
ORIGIN      
        1 agcaaccggc tcataaccgg ttggtccggg gttcgagtcc ctgaaggccc accaggagag
       61 caaggattca tttccttgct ctaatttttt acgtcctttt atcagccaga tcgtcgaaaa
      121 tgcggtcctg ctgcatatta gcctgaaccg tcattttccg ggattccctg tcagtccttt
      181 ttggcacgcc ggccgctgaa ctgaacggta tgatcgtttc ccgcgctgtt tcaacatcgc
      241 ttgcatgttt gcgtgaaaaa ttccttccca tcatatctcg ctcctaaccg gaaaagattt
      301 ggtgtataat taagcatgta caaagtgcag atttttataa cggtaaggaa aatacggacg
      361 gagagaaata tgaaactgga cggaaggctc ggcatgatag ctgaaaatat accgcggtgc
      421 agcatcctgg cggatgtcgg cactgatcac gcattcatac ccatatatgc agtggaggca
      481 tccctgtgtg agagggctgt tgcagcagat gtaaggcccg gacctctcac ggctgccagg
      541 aaaaatattg caaaacatgg actggaaagc cgtatcgaaa cgaggcttgg agacggactg
      601 gggccgatat gcgaaccgga gtgcgacgta gttgtaattg cagggatggg cggccctctg
      661 atcagaagga tattggcgga atcatcagaa aaggcaaaaa agtgccggct gttgctgctg
      721 cagccaaaca atgcgccgga agccctgcgc aaatggcttt atgaaaactg cttcagcatc
      781 atcggcgagg atcttgcaga ggacagcggg aaactgtatt gcctgatcaa ggccaaatgg
      841 accggtacat atgaaaaacg ggatgatttc gactgctata taggcaggaa gctcctcgag
      901 agcgatgatc cgcttatcat accttacctg aagaagaaac tcggtgaact ggacactata
      961 atagacggac ggtcgaagtc agatccggga aagccacgca gggtcgaata cgattcctac
     1021 atggatacga agacgtgtat ggaaataagg gacaggctcc gggcctatct tgacgcgaga
     1081 tgaccgggac aagtctgcgg ggtatgcctg tggccatgat acggaacagg catgcctgcg
     1141 ggaacaacca ggcttaaaat aaagataaaa tgagggatac cggattagaa aagtaacgga
     1201 aaggagaaat tttcatgata acatgcggcg atgtcatgga tttccttcag gaacttgcac
     1261 ctgccgaact cgcggaagat tgggacaata tagggcttat gacaggcggc aggaaaagga
     1321 aagtcggcag gatccttgtc tgtcttgatc cgacagcgga tgcgcttgat aaagcggcag
     1381 aaataggggc agacctttta gtgacccacc attccctgat tttcaaaggg ctgaaaagcg
     1441 tgaacgatga tgatattgtc gaaagccgcg tatacactgc catcagcaaa ggcataagcg
     1501 ttatctcggc ccacacgaac cttgactatg cagacagcgg agtaaacgtg caactggcat
     1561 ccgttttagg cttgtgtgat ctggaggtgc ttggccatgg tcccggaagg gtgggtatgc
     1621 tgaaggaaag gacagacctt gaaacgttca taagccatgt gaagacatct cttggtatat
     1681 cccacgtaag agtggccggc aatgccgcgt caggcgtgcg gaaggtcgca gttttcggcg
     1741 gaagctttga tggcgatctg gatgctgtaa agagatcatg tgctgatgtt gttgttacag
     1801 gggaaatgaa acaccatact gcgttggatg ttcgtgaagc aggcctgtgt tttatagtcg
     1861 ccagccattt cagcaccgaa agagtagtgg tgccatatct tgcatctgcg atttcaggca
     1921 ggttcccgtc ggtcgaggtg atatgtttcg atcaggagaa ggaccctttt attacttatt
     1981 gacttaacac ggcttatgga ttatcataaa tccggaaaac aaaatatgtg agtaagtcgg
     2041 ataatcgcgg gtacagtgcc gaaaggccgt atctgaggaa agtccgggct ccgcagggca
     2101 gggtgccggg taactcccgg tgaaggcgac ttcaaggaaa gtgcaacaga aatataccgc
     2161 tcagcgtgtg atgtgtcaag catcaatgct gagtaagggt ggaaaggtga ggtaagagct
     2221 caccagcggc atggcgactt gccggtctat gtaaacccca cccggagcaa caccgcagag
     2281 ggacatatgg tggcccgcca gtcccgggca ggtggcttga gccatgcaga aatgtatggc
     2341 ccagatagat gattatccac gacagaaccc ggcttacaga cttactcatc aaatatatca
     2401 aagacaccgg ctttaaggcc ggtgtctttg tgtcggtaca aatttgatat ccgatttgaa
     2461 attccgggca gccgggtcat tcgagcttga atctggcaat ggtctgctgc agttcttcgg
     2521 aagatgcttt aagctcgtca gcgaaccggg acagttcttc aatgaatgaa agctgttctt
     2581 cagtggaggc tgtgacttcc tgcgtggaag cagcagtttg ctgggaaacg gccgaaatgt
     2641 tctggatgga atttatggcc tgtgtcttgt tttcttccat ttcggcgact ccggacatta
     2701 ttctctccac ctgctcagaa aggttcccca tagatcccat tatcctgttg aaaatctgta
     2761 tcgttccctg tacagcttca ttctgggatt taaggtaagc ttcggtatca tttgccttgt
     2821 ctgccgtttt agccgtaagg tcctgtgtgg acttgatgat attggcgatc tcacgggcag
     2881 attccattga catctccgcc agtttcctga cttcgtcggc aacgacagca aagcctttgc
     2941 cggcttctcc ggccctggct gcttctatcg cggcattgag cgacagaaga ttggtctggt
     3001 cggcaatacc gctgatgacc tttattattt ttccaatgga tcttgattgc gcttccaggt
     3061 tcttgatgtc ttcaataatc tctcttgtga tgtctgttgt ccgtccggcc ttgacgtcaa
     3121 gatcctctac cgtggcaagc ccggtctgtg taagggactt tgtgtcgcct gtcagttcgt
     3181 ctatcagctt tgcgcttttt atcacatcat ttatcttctg atccagtatg cttatcttcc
     3241 ttacaccctg ttccgcatcg gcagcctgtg cgccggctcc ggtcgctatt tcctgtattg
     3301 cccgcgttat atccttcgag acgttggata cctgctgtga agtcgatgaa acgatctccg
     3361 ccgatcctgt gactttttca gcgacttcag atgtctgtac gataaggttt ctcatactgg
     3421 ctatcatgga acttatgctt ctggtaagtt caccgagttc atccttcctc cttgactgca
     3481 gagtaactgt cagatctccc gaagctgccc tggcggatgc gcttatgatc ctgtttatcg
     3541 tcctgcccat gctgttggcc atcagtatac ctgctgcaaa tgcaataatg actgcggcaa
     3601 cacatgtaag tactgtagag attatgatac gattcgccga tgcttcgagg atactgtcag
     3661 ggatcaggtt tatcaatgtt atgccggtcc tggagatctt cctgaaaacc atgaagaagt
     3721 ctttcccgtc cattgacact gtgtcggcgc caatggcttc atcagaaccg agcagctttt
     3781 tataaaaatc ctgttgcgta atggtgctgg tatcagtctc gtcaacagca ttgacgacga
     3841 ccctgttgtc aggggtcacc ataatgaact gctggccttc atagattatc tgagactcga
     3901 acaattcaga gaccacgtcg gcattgacat caattacgag aatccctatg ctcttcgcag
     3961 ttttcgtgct gcggatcagc caggcgaatg acagtgaata cgaatcgggc gacacttcca
     4021 gtatcgcatc cagttcctca tgcctgccga accatgccct tgacgactcg atagattcac
     4081 gggtttttac aagatttgat tctgcgat
//
LOCUS       DAOSCC010000214         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_13836_length_4084_cov_4.229586, whole genome shotgun sequence.
ACCESSION   DAOSCC010000214 DAOSCC010000000
VERSION     DAOSCC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13836_length_4084_cov_4.229586"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLG72_09955"
     CDS             <1..537
                     /locus_tag="PLG72_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOJ81159.1"
                     /translation="LTLPKDVFSAFMIANGGYMGSVLFALLILKLKKTPARKYILGAC
                     AILLLGMSIAYGDSVFTMICAAIFAGLVIIIYMLRNDKVNDWVIDIIGAVCAVYAVYD
                     TFIDTILLEINRKLGLIPGWGETRPVTDAVQLELMTHVPAVVWGVIWFIIACCAIYST
                     MLGKRKRPAGARGRRTAA"
     gene            614..2584
                     /gene="ligA"
                     /locus_tag="PLG72_09960"
     CDS             614..2584
                     /gene="ligA"
                     /locus_tag="PLG72_09960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ81160.1"
                     /translation="MDKIRLMKEKIELLNKAAKAYYQENREIMSNYEYDRLYDELVEL
                     EKETGMVLSNSPTVRVGYELLTALPKERHEKPMLSLDKTKDVEVLKSWLGDRKGLLSW
                     KLDGLTIVLTYSDGTLAKAVTRGNGEVGEVVTNNVKVFKNIPLVIPFKGDLILRGEAV
                     ISYSDFNRINEEMIDATIKYKNPRNLCSGSVRQLNNKVTSERNVNFYAFSLVKADNVD
                     FGNSRAAQLEWLRSQGFDVVEYRMVDAGTVEEAVKWFAGHVEGYDIPTDGLVLAFDDI
                     AYGESLGATSKFPRDSIAFKWRDEIKETVIKEIEWSASRTGLINPIAIFEPVELEGTT
                     VSRASLHNISIMEELQIGIGDTVTVYKANMIIPQIYENLTRSSDLRVPDVCPVCGGPA
                     EIRQNSDVKYLYCPNRNCAAKHIKKFTHFVSRDAMNIEGLSEATIEKFVAAGLVREYA
                     DLYRLAEHRDRIVGMEGFGEKSFANLIESVDRSRKTTAVRLLYGLGIPNIGLSNARLI
                     CRRFEHDWSRIENATFEQLTEINGIGDVMADAFVRYFSDEENRAAVNSIIREVELEKP
                     DTPANVQLLENLTFVITGSLERFANRKELTELIESMGGKVSGSVSAKTSYLINNDIAS
                     YSTKNKTAKELGIPIITEAQFLDWVENGNRPQ"
     gene            complement(2779..>4084)
                     /gene="glnS"
                     /locus_tag="PLG72_09965"
     CDS             complement(2779..>4084)
                     /gene="glnS"
                     /locus_tag="PLG72_09965"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOJ81161.1"
                     /translation="DLSPDEIREFRGTLTEPGKESPYRNRSVEENLDLLERMKAGEFP
                     EGSKVLRAKIDMASPNMNMRDPVIYRIMKVDHWRAGKKWCIYPMYDFAHPIEDAIENI
                     THSLCSLEYEDHRPLYDWVVNNVDLGSKPRQIEFARLNLTYTVMSKRKLLQLVNEGYV
                     SGWDDPRMPTLSGLRRRGYTPASIWDFCERIGVAKTNSTVDYGLLEHCVREDLNASAP
                     RVMAVLNPLKVIIDNYPDDQVEWFDVEVNPEKPEMGTRKVPFCREIYIEQDDFMENPP
                     KKYFRLYPGNEVRLKNAYYIKCSHAVKDTEGNIIELHCTYDPESKGGSTPDGRRVKGT
                     LHWVSARHAIDAEVRLYDHLFTVPDPGAAEDISTIINPDSLIVLKHCKMEPTLADTKP
                     GDHFQFLRMGYFCTDPGSEPGKLVFNRTVGLKDSWSKEMNRQ"
BASE COUNT         1026 a          998 c         1098 g          962 t
ORIGIN      
        1 cttactcttc cgaaagatgt attctccgcg ttcatgatag caaacggagg ctatatgggc
       61 agcgtcctgt ttgcgctgct tatactgaaa ctgaaaaaga cccccgccag gaaatacata
      121 ctcggcgctt gtgcaatact gctgcttggc atgtcgatag cgtacggcga ttccgttttc
      181 acgatgatct gcgcagccat tttcgccggg cttgtgataa ttatttatat gttgagaaac
      241 gacaaagtca atgactgggt catagacata atcggagcgg tttgtgctgt ctatgcggtt
      301 tatgacacgt ttatcgatac catactcctt gagatcaacc ggaaactcgg cctgataccg
      361 ggctgggggg agaccaggcc ggtcacggat gcagtgcagc ttgaacttat gacacatgtc
      421 ccggcagtgg tatggggagt gatatggttc attatcgctt gctgtgcgat ttactcgaca
      481 atgctcggga aaaggaagag accggcaggc gcaaggggaa gacggaccgc tgcgtgatat
      541 cacccaagac ataacatcag taatgatgga tggatgtctg tcctggcaat aaatcaagtt
      601 tcggaggtat cctatggaca agatacggct gatgaaagag aaaatcgagc tgcttaacaa
      661 ggcagcaaaa gcgtattacc aggaaaaccg cgagataatg tccaattatg agtacgacag
      721 gctctacgac gaactggtcg aacttgagaa agagaccggg atggtgctgt ccaacagccc
      781 gactgtccgc gtcggctatg aactgctgac agcgctgccc aaggaacggc atgagaagcc
      841 gatgctctcc ctggataaaa ccaaggatgt cgaagtattg aaatcatggc ttggggaccg
      901 gaaaggcctg ctttcatgga agctcgacgg ccttaccatc gtactgacat acagtgacgg
      961 aactcttgca aaggcagtca caagaggcaa cggcgaggtc ggcgaagttg tgacgaacaa
     1021 cgtgaaggtt ttcaaaaaca tccctctcgt catcccgttc aagggagacc tgatcctgag
     1081 gggcgaagcc gtaataagtt attctgattt caacaggatc aacgaagaga tgatagacgc
     1141 aacgataaag tacaagaatc cgaggaatct ctgcagcggt tcggtacgcc agctgaacaa
     1201 caaggtgact tccgagagga atgtgaattt ctacgcattt tcgctggtaa aggccgataa
     1261 cgttgatttc ggcaattcaa gggccgcgca gcttgaatgg ctcaggtccc aggggttcga
     1321 cgtcgttgaa taccggatgg tcgatgcggg aaccgtcgag gaggccgtca aatggttcgc
     1381 cgggcatgta gaaggatatg acatacctac cgacgggctt gtcctcgctt ttgacgatat
     1441 cgcatacggc gaatcgctgg gggcgacatc gaaattcccg agggattcca tcgctttcaa
     1501 atggcgtgat gaaatcaagg aaactgtgat aaaggagata gagtggagcg catcgcggac
     1561 cgggctcatt aatcccatag ccatattcga acctgtggaa ctggaaggca ccacggtgag
     1621 ccgtgccagc ctgcataaca taagcatcat ggaagaactg cagataggga tcggcgatac
     1681 tgtcaccgtg tacaaagcca acatgataat ccctcagata tatgaaaacc tgacaagaag
     1741 ctcggatctc agggtgccgg acgtatgccc ggtgtgcggc ggccctgccg aaataaggca
     1801 gaacagcgat gtgaagtatc tctactgtcc caacaggaac tgtgcggcca agcacatcaa
     1861 gaagttcacc cattttgtca gcagggacgc catgaacata gaagggcttt ccgaggctac
     1921 catcgaaaaa tttgtcgccg cgggcctggt cagggagtac gcagaccttt accgacttgc
     1981 tgaacacagg gacaggatcg tcgggatgga aggattcgga gagaagtcct ttgcaaatct
     2041 catcgaatct gtggacaggt cgaggaagac taccgccgtg aggctccttt acgggctggg
     2101 catcccgaat atcggccttt ccaatgccag gctcatatgc cgcaggttcg aacatgactg
     2161 gagcaggatc gaaaatgcga cattcgagca gctaacagag ataaacggga taggggacgt
     2221 aatggcggat gctttcgtac gctatttcag cgacgaagag aacagggcgg ccgtaaacag
     2281 cataattagg gaagtggaac ttgagaaacc ggatactcct gcgaatgtac aactgctcga
     2341 aaatcttacc ttcgtgatca cgggttcgct ggagcgcttc gcgaacagga aggaactcac
     2401 ggaactcata gagagcatgg gaggaaaagt gtcggggtcc gtgtcggcga agacgagcta
     2461 cctgatcaac aacgatatag cgtcctactc aacgaaaaac aaaacagcaa aggaacttgg
     2521 catacccata atcacagagg ctcagttcct cgactgggtc gagaacggga accggccgca
     2581 ataaaaaact gacggcacaa gaacagccac tgctgcgggt cagtgtgcgg aagatccatg
     2641 aacagcaggc tgaatgagga gttcagacta tataaaacat cggaccgtaa ggcttagcct
     2701 tcacggtccg atgcttttga ttcctcatgt cgacggtttg ttgagattta catgttgccg
     2761 gatgattaca catctctgtt attgcctgtt catttccttg ctccatgaat ccttgaggcc
     2821 gacggtcctg ttgaatacaa gttttccggg ctcggaacca gggtccgtgc agaaatatcc
     2881 catgcgcagg aactggaaat ggtcgcccgg cttcgtatct gccagggtcg gctccatctt
     2941 gcagtgcttc agcacgatca gtgaatccgg gttaatgatc gtactgatat cttctgccgc
     3001 ccccggatca gggactgtga aaaggtggtc gtacaggcgt acttccgcat cgatggcgtg
     3061 tcttgcagat acccagtgaa gcgtaccttt gaccctgcgg ccgtccggcg tgcttccgcc
     3121 cttcgattcc ggatcgtaag tgcagtggag ttcgattata tttccttcgg tatccttgac
     3181 ggcatgcgag cacttgatgt aatatgcgtt tttcagcctc acctcgttgc ccggataaag
     3241 cctgaaatat tttttcggcg ggttttccat gaagtcgtcc tgctctatat atatctccct
     3301 gcagaacggg acttttctcg ttcccatttc aggcttttca ggatttacct ccacgtcgaa
     3361 ccattcgacc tggtcatcgg gatagttgtc tatgatgact ttcagcggat taagtactgc
     3421 catcactctt ggagccgaag cgttgaggtc ttcacggacg caatgctcca gcaggccata
     3481 gtcgacagtg ctgtttgtct ttgctacgcc gatcctttca caaaagtccc atatggacgc
     3541 cggtgtatag cctcttctcc tcagtcccga cagcgtaggc atcctcggat cgtcccagcc
     3601 tgatacatag ccttcgttta ccaactgcag cagttttctt ttgctcatca cggtatatgt
     3661 gaggttcaga cgcgcaaatt ctatctggcg cggcttcgaa ccgagatcaa cgttattcac
     3721 gacccagtcg tagagcggcc tgtggtcttc atactcaagc gaacagaggg aatgggttat
     3781 attttctatg gcgtcctcta tcgggtgcgc aaaatcatac attgggtaga tgcaccactt
     3841 tttgcccgcg cgccagtgat ctaccttcat gattcggtag atgacaggat ccctcatgtt
     3901 catattcgga gacgccatgt cgatctttgc gcggaggact tttgagcctt cggggaattc
     3961 tcctgctttc atccgctcga gcaggtccag gttctcttcg actgaacggt tcctgtaagg
     4021 gctttccttt ccgggttctg tcagcgtgcc cctgaattcc ctgatctcat cggggctcag
     4081 gtcg
//
LOCUS       DAOSCC010000215         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_14080_length_4031_cov_4.421529, whole genome shotgun sequence.
ACCESSION   DAOSCC010000215 DAOSCC010000000
VERSION     DAOSCC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14080_length_4031_cov_4.421529"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /gene="rho"
                     /locus_tag="PLG72_09970"
     CDS             <1..1299
                     /gene="rho"
                     /locus_tag="PLG72_09970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ81162.1"
                     /translation="AESADTARQDKPAATARSVSQDEGRPAEESRTEERLTVPAQPRR
                     DWNGQERQYNGLERIESDDPVEGVLEVLPDGYGFLRSDNFLSGTRDVYVSPSQIRRFG
                     LKTGDKIKGKGRIPKEGEKFQALLYVQSVNGDPPEVAARRIPFEQLTPIFPNQRITLE
                     TDPKELSTRLIDLIAPIGKGQRGMIVSPPKAGKTILLKKIANAITRNYPEIELIVLLI
                     DERPEEVTDMQRSIKGEIIYSTFDKVPENHIKVAEMVLERAQRLVEHKKDVVILLDSI
                     TRLARAYNLTIPPTGRTLSGGLDPGALHSPKRFFGSARNIENGGSLTIIATALIETGS
                     RMDDVIYEEFKGTGNMEIHLDRKMSEKRIFPAIDINRSGTRREELLLNPKELESIWAI
                     RKAMSNLGPADVTEMILNRMIQTKTNEEFVNSINKAFIDK"
     gene            1423..1635
                     /gene="rpmE"
                     /locus_tag="PLG72_09975"
     CDS             1423..1635
                     /gene="rpmE"
                     /locus_tag="PLG72_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOJ81163.1"
                     /translation="MKENIHPQYYDNAVLKCACGETFVTGSTKKELHVEICSKCHPFF
                     TGKQKLVDTGGRVEKFKKKFGIEDAE"
     gene            1774..2700
                     /locus_tag="PLG72_09980"
     CDS             1774..2700
                     /locus_tag="PLG72_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ81164.1"
                     /translation="MKKTNIGGQALIEGLLMIGPENIAIAVRKPEGEIVLDKKPAPLK
                     TKVTKIPFIRGSVNLFRQMVTGVRALMYSAEFAELEDEGNNEPSKLDAFIERIFKGKG
                     KDAVIYFTVILSLALSIGLFILLPNFLVSLLNIAKEGSGVILKNLLEGLVRIVIFFAY
                     IWLASLMKDIRRVWQYHGAEHKTIHCYEHGDELTVENVRKYSTKHPRCGTSFLFLVMI
                     ISILMFSLVGWHSPVVNMLLRILMIPVVAGIAYEVLKFSGKHTEWPVMRIVSAPGMLL
                     QYLTTREPDDDQIEVAITAMKNVIVDDDSDRW"
     gene            2835..3809
                     /gene="prmC"
                     /locus_tag="PLG72_09985"
     CDS             2835..3809
                     /gene="prmC"
                     /locus_tag="PLG72_09985"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ81165.1"
                     /translation="MTYGEAYRKGMEILKNAGIDAPANDAGVLLCYAAGCSRTHLYAY
                     GDSVLPEQIERRYFELLEKRSGSYPLQYITGEQEFMSLVFEVTPDVLIPRQETELLVE
                     TVLDYCRIRVLAENDEHVEQEALAKEKLSGRPDGYGKSDRHAGRRRPVEILDMCTGSG
                     CIAVSLARYLPGCIITAVDLIPAALAVAGRNAERHGVSGRVRFIRSDLFGSIPEKKYD
                     VIVSNPPYVRTEDICKLQPEVKFFEPVAALDGGSDGLRFYREIIRKSPGYLRMGGMLA
                     FETGYDQAKAVAGLMESDGRFSYIRIHRDLSGTDRVVSGFTSAQAAPL"
     gene            complement(3781..>4031)
                     /locus_tag="PLG72_09990"
     CDS             complement(3781..>4031)
                     /locus_tag="PLG72_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ81166.1"
                     /translation="KDGTVSLSSGDRFFMYTDGILEIRNKANEQFGEDRLLEILLNSD
                     PRPEQILLDIKESAFRFAGIGSSNDLHDDVTMALLELK"
BASE COUNT         1139 a          898 c         1134 g          860 t
ORIGIN      
        1 gctgaatctg ctgatacggc cagacaggat aagcctgcgg caacggccag gtctgtgagc
       61 caagacgaag gcagaccggc tgaagaaagc aggacagagg aaaggctgac tgtccctgcg
      121 cagccgagaa gagactggaa cggacaggaa aggcagtaca acggcctcga gagaatagaa
      181 agcgacgacc ctgtggaagg cgtgctggaa gtgctgccgg acgggtatgg cttcctgagg
      241 agcgataact tcctttccgg tacgagggac gtctacgtgt ctccttcgca gatcaggaga
      301 ttcggcctca agaccggcga caagatcaag ggtaagggaa ggataccgaa agagggcgag
      361 aaattccagg ccctgctcta tgtgcagtcc gtcaacggag atcccccgga agtcgcagcc
      421 aggaggatac ctttcgagca gttgacgccg atattcccca atcagcgcat aactctggaa
      481 acagacccta aagagctgtc cacgaggctt atcgacctga ttgccccgat agggaaagga
      541 cagaggggaa tgatcgtatc tcctcccaaa gcgggcaaaa ccatactgct taaaaagata
      601 gccaatgcga taacaaggaa ttatcccgag atagagctga ttgtgctgct gatcgacgaa
      661 aggcccgagg aagtcacaga catgcagcgc tccataaagg gagaaatcat atattccacg
      721 ttcgacaagg tacccgagaa ccatataaaa gttgcggaaa tggtactgga aagggcacag
      781 aggctggtcg agcacaagaa ggacgtagtg atcctgctcg acagtataac aaggctggca
      841 agggcataca acctgactat acctcctacg ggaaggacac tttcaggcgg acttgacccg
      901 ggcgctctcc acagccccaa gaggttcttc ggttcagcgc gcaacatcga gaacggcgga
      961 agccttacga tcatagccac cgcactgata gagacaggca gccgcatgga cgacgtcata
     1021 tacgaggaat tcaaaggcac gggcaacatg gagatacatc tcgacaggaa gatgtctgaa
     1081 aagaggatat tcccagccat cgacatcaac aggtcgggaa cgcgaagaga ggaattgctg
     1141 ctcaacccga aggaactgga aagcatatgg gctatcagga aagccatgag caatcttggc
     1201 cccgcggatg taacagagat gatcctaaac aggatgatac agaccaaaac gaatgaagag
     1261 ttcgtgaaca gcatcaacaa ggcgttcatt gacaaataaa cgatatacgc agcaaatagg
     1321 acttgctaaa ggcagctata tgtgttagaa tgttttttgt ttgttgattg agcaaaatgg
     1381 caaaaactat tttgcaacag gacgaaggca ggtgaaatga taatgaagga gaacatacat
     1441 ccgcagtatt atgataacgc agtattgaag tgcgcatgcg gcgagacctt tgtcacaggt
     1501 tccacgaaaa aggagctgca tgttgaaata tgctcgaaat gccatccgtt ctttaccgga
     1561 aagcagaagc tcgtagatac cggcggacgt gttgagaagt tcaagaagaa attcggtatc
     1621 gaagatgctg aataaaaaca atgggatttt tttgggctgc attgcccgga aacaaatgaa
     1681 gtttgaaatg gagatacaaa gatgataaac taaaggctga atagacagtt cagtctttat
     1741 tttttattta aggccgaagg agttagcaaa tacatgaaaa aaacgaatat aggcggccag
     1801 gcccttatag aagggctgct gatgataggt ccggaaaaca tcgccatagc ggtcaggaaa
     1861 ccggaaggag agattgtgct ggacaagaag ccggcgccgc taaaaaccaa agtgaccaaa
     1921 atacctttta tccggggatc tgtcaacctg ttcaggcaga tggtcacagg cgtgagagcg
     1981 ctcatgtact cggcggagtt tgccgaactt gaggatgaag gcaataatga accgtcgaaa
     2041 ttggatgcct tcattgaaag gattttcaaa ggcaagggca aggatgcggt catatatttt
     2101 accgtgatcc tttcacttgc cctgagtatc ggcctgttca tactgctgcc aaacttcctg
     2161 gtaagtcttc tcaatatagc gaaagagggc tccggcgtga tactgaagaa cttgctggaa
     2221 ggcctggtca ggatcgtgat atttttcgca tatatctggc ttgcgtcttt gatgaaggac
     2281 atcaggagag tatggcagta ccacggcgcc gaacacaaaa ccatccattg ttacgaacac
     2341 ggtgacgagc ttaccgtgga aaatgtgaga aaatattcga cgaagcatcc gcgctgcggg
     2401 acatcgttcc tgttccttgt aatgataatc agcatactca tgttttcgct ggtcggatgg
     2461 cacagccctg ttgtcaacat gctgctgcgc atcctgatga tccctgttgt tgccggcatt
     2521 gcctacgagg tactgaagtt ttccgggaaa cacacggagt ggccggtgat gcggatagtc
     2581 agcgcacccg gcatgctcct gcagtatctt accacaaggg agccggatga tgaccagata
     2641 gaagtggcta taacggcaat gaagaatgtt atcgtggatg acgattccga caggtggtaa
     2701 catatacctt gcgacagttc cgaaaatgcc cacccgggca tgcggacaag ctgccgcacg
     2761 aaagcctgtt aaggcaggga atatatctct gaagtataaa atgaagcata atcggcgtat
     2821 cattgttaat tggtatgact tacggtgaag cgtatcgaaa aggcatggaa atattgaaga
     2881 atgcggggat cgatgctccg gcaaatgatg ccggagttct gctttgttat gccgcgggat
     2941 gcagcaggac tcatctgtat gcgtatggtg acagcgtact tccggagcaa atcgagcggc
     3001 gttattttga actcctggaa aagcggtccg gaagttatcc gctgcagtat attaccgggg
     3061 aacaggaatt tatgtcgctc gttttcgagg ttactcccga tgtcctgata cccaggcagg
     3121 aaacggagct actggtggaa acggtgctgg attactgcag gataagagtt ttagctgaaa
     3181 atgatgagca tgtggagcag gaagctttgg cgaaggagaa attatccggc agacctgacg
     3241 gatacgggaa atctgacaga catgccggac gcagacggcc ggtcgagatc cttgacatgt
     3301 gcacgggatc aggctgcata gccgtaagcc tggcgcggta tcttcctggc tgcatcataa
     3361 cggcggttga cttaataccc gcagcactgg cagtagccgg gagaaatgca gagaggcacg
     3421 gcgtgtccgg aagggtccgc tttatccgga gcgacctgtt cgggagcatc ccggagaaaa
     3481 agtatgacgt aatagtttcg aatcctccat atgtaagaac ggaagatatc tgcaaacttc
     3541 agcccgaggt gaagtttttc gaacctgttg cggcgcttga cggcggcagt gacggcctcc
     3601 gcttctacag ggagataata cgcaaatccc ctggttattt gcggatgggg ggaatgctgg
     3661 cattcgaaac cggatacgac caggcgaagg cagtggccgg ccttatggaa tcggacggaa
     3721 ggttttcgta tatccggata cacagggatc tgtcggggac cgacagggtc gtgtcagggt
     3781 ttacttcagc tcaagcagcg ccattgtgac atcatcgtga agatcattcg atgatccgat
     3841 acccgcgaac ctgaacgcgg attcctttat atccagcagg atctgttcag gcctgggatc
     3901 gctgttgaga agtatttcaa ggagcctgtc ctctccgaac tgttcgttgg ccttgtttct
     3961 gatctcaagg atgccgtccg tgtacatgaa gaacctgtcg ccggatgaca ggctgacagt
     4021 gccgtcctta t
//
LOCUS       DAOSCC010000216         4010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_14163_length_4010_cov_3.791150, whole genome shotgun sequence.
ACCESSION   DAOSCC010000216 DAOSCC010000000
VERSION     DAOSCC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14163_length_4010_cov_3.791150"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2001)
                     /locus_tag="PLG72_09995"
     CDS             complement(<1..2001)
                     /locus_tag="PLG72_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ81167.1"
                     /translation="MSETAINKSFTEVFSDLRLDDAVSDFFSEVRIDNIGIYKKTGKL
                     VIKASADRLIPARVTEQVETALSEAFSLDVEIQLRFETDGPLTEILEEYRDSILHIVN
                     SRVALSKGILDGCTWKLEGDRIEIMLATKGRDILKSHCCDRLIEEIVEHSFGKKVSVD
                     FHDRKVDEEFTEKYNEFKENEQARIRSLLTAEEAQLVKHRKRRKANTDDTNVIDIITG
                     KNFSDSLMKMCDVTPDSGKVAICGDVLNTEFREVRGNQYLCTFDVTDYTSSITVKLFI
                     QKDDRDIIEEHIKPGVHLKVRGDAQFDKFSRDLTVLASDIVELRKAVKTDNAEVKRVE
                     LHLHTQMSAMDAVTPVEKLVERAAQWGHKAIAVTDHGVVQAYPDAYSAGKKHNVKIIY
                     GVECYLVNDEIPVVTAGDGAETGHPLTGGFVVFDIETTGLNPSKDGITEIGAVKVENG
                     KVAGSFSTFVNPGMPIPEHITRLTGITDAMVADAPGISQAVGDFLDFAGGLPVVAHNA
                     SFDTGFIRYHARQSGRELNNVIVDTLQLSRKLFPELSRHKLDIVARHLGVSLENHHRA
                     VDDAQATAEIFIRCMEIVAEKGAATVGDIDKVLSGETDLKNLPSYHAILLVKDRTGLK
                     NLYRIISESHLNYFYKRPRVPRSILMKYREGLILGSACEAGEL"
     gene            complement(2050..3117)
                     /gene="ispG"
                     /locus_tag="PLG72_10000"
                     /gene_synonym="gcpE"
     CDS             complement(2050..3117)
                     /gene="ispG"
                     /locus_tag="PLG72_10000"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOJ81168.1"
                     /translation="MGRIERKKTRKIRIGDIYIGGDAPVAVQSMTNTDTRDVSATVDQ
                     IKRLEEAGCDIVRVAVPDSEAAEALKRIRKSIRIPLVADIHFDYRLALASIENGADKI
                     RINPGNIGGTEKVRKVVEAAKARGIPIRIGVNSGSLEKHILAKYGAATPQAMADSAMG
                     HVRMLEDLGFHDIVISLKASNVPVTIESYRLMSAMTDYPLHIGVTEAGTLFSGMVKSA
                     AGIGCLLAEGIGDTLRVSLTGDPVEEVRVGIEILKALDLRRTGVELISCPTCGRTRID
                     LVKIANEVEKRLAGCSKPIKVAVVGCAVNGPGEAREADIGIAGGVGEALLFKKGRIIR
                     KIPQEHIVDELLEEIERMQIT"
     gene            complement(3150..>4010)
                     /locus_tag="PLG72_10005"
     CDS             complement(3150..>4010)
                     /locus_tag="PLG72_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ81169.1"
                     /translation="KRIYAPIDVVQFLYVSKGEPVTVEYKRDGKLLKGELRPIYHPET
                     SSPMMGVVLDLTNPEGSNVLKEIYAGSPAEQAGLMAGDKVVAINGTEVDSYEKMTELI
                     KNNGINELEITVLRNGDHFVTTLTPIEVKTEEWYEVGLDFGFSRGDVLDALGQSAIFT
                     YSIIRSVYYSPKWLITGKVNVSDMMGPIGMVSTITTTVSQAPNVGQMFLYLMYITGML
                     NVALGATNLLPFPALDGGRLLLIGIEAVRGKPLNPEKESVITMAGFIIIILFGIYIAV
                     NEFQKYVIGS"
BASE COUNT          800 a         1158 c          903 g         1149 t
ORIGIN      
        1 cagttccccg gcctcacagg cgctgccgag tataaggcct tccctgtatt tcatgagaat
       61 actccggggt accctgggcc tcttgtagaa ataattcaga tgtgactccg aaatgatcct
      121 gtacaggttt ttgagcccgg tcctgtcttt gacaagcagt attgcatggt atgacggcag
      181 attcttcagg tcggtctctc cggacaggac cttgtcgatg tctccaacgg tggcggctcc
      241 cttttcagcc acgatctcca tgcacctgat aaagatctcg gccgttgcct gggcgtcatc
      301 cacagccctg tggtggtttt ccagcgagac gccgagatgc ctggccacta tatcgagctt
      361 gtgcctgctg agctcaggga acagcttcct gcttagctgc agcgtgtcca ctatcacatt
      421 gttcagttcc ctgccgctct gccgcgcatg gtacctgata aagcccgtat caaaggaggc
      481 attgtgggct accaccggca atcccccggc aaaatccagg aaatccccga ctgcctggct
      541 tatcccggga gcatcggcaa ccattgcatc ggtgattccc gtgagcctcg tgatatgctc
      601 cggtatcggc atgccgggat tgacgaacgt gctgaaactg ccggcgacct ttccgttttc
      661 cacttttacg gcgcctatct ccgttatgcc gtctttggat ggattgagtc ctgtggtctc
      721 tatatcgaac accacaaagc cgccggtcag cggatgtcct gtttctgcgc cgtcgccggc
      781 ggtcacgaca ggtatttcat cattgacaag ataacattcc actccgtata tgattttcac
      841 attgtgcttt ttcccagcgc tgtacgcatc cgggtacgcc tggacaacgc cgtggtcggt
      901 gaccgcgatg gctttgtggc cccactgtgc cgccctttcc accagctttt ccaccggcgt
      961 gacggcatcc atcgcgctca tctgtgtatg caggtggagt tcgacccttt ttacttccgc
     1021 attgtctgtc tttacagctt ttctgagttc gactatgtct gaagcgagca cggtcagatc
     1081 tctcgagaac ttgtcgaact gtgcgtctcc ccttactttc agatgaacgc ccggctttat
     1141 atgttcttct atgatatcac ggtcatcctt ctgtataaag agtttgacag tgatggaact
     1201 ggtatagtcg gtaacgtcga atgtgcacag atactgattc ccgcggacct ccctgaattc
     1261 cgtgttcaat acgtcgccgc agattgccac tttgccggaa tccggcgtta cgtcgcacat
     1321 tttcatcagg ctgtcgctga agttttttcc tgtgattata tcgatcacat tggtgtcgtc
     1381 cgtatttgct ttccttcttt tcctgtgttt gaccaactgt gcttcttcgg cagtcagcag
     1441 gcttctgatg cgcgcctgtt cgttctcctt gaattcgtta tatttttccg tgaattcctc
     1501 atccaccttg cggtcatgga aatccacgct gaccttcttc ccgaaggagt gttcaacgat
     1561 ttcttctata agcctgtcgc agcagtggga tttcaatatg tcccggcctt tagtcgccag
     1621 cattatctcg atccggtcac cttcgagttt ccaggtacat ccgtccaata tgcctttgct
     1681 cagcgcgacc ctggaattga ctatgtgcag tatgctgtca cggtattcct ccaatatctc
     1741 cgtaagaggg ccgtctgttt cgaaacgcag ctgtatttca acatccagtg aaaaagcttc
     1801 cgacagcgcc gtctcgacct gttccgtgac ccgggcaggt atgagcctgt ctgcagaagc
     1861 tttgatcacc agttttcctg tttttttgta tataccgatg ttgtcgatcc ttacctcgct
     1921 gaaaaaatca gatacagcgt catccaaccg cagatcacta aaaacttcag taaagctttt
     1981 gtttattgcc gtctccgaca tctgacagat ccccttttgt aagttgatgt acaattgtca
     2041 agtcagttat caagttatct gcattctctc tatctcttcc aggagttcat ccacgatatg
     2101 ctcctggggt atctttctga tgatcctgcc ttttttgaac agcaacgctt ccccaacgcc
     2161 gcccgcaata ccgatatcag cttcccgggc ttctccgggt ccgtttaccg cgcagcccac
     2221 gactgcgact ttgattggtt tgctgcatcc ggcaagcctt ttttctactt catttgctat
     2281 tttaaccagg tcgatcctgg tgcgcccgca tgttgggcag gatatgagtt ccacccctgt
     2341 tctgcgaaga tccagggctt tcaggatctc gatccccact ctcacttcct ccaccggatc
     2401 gcctgtcaga gatactctaa gcgtatcccc aataccttcc gccagaaggc atccgatacc
     2461 tgccgcagat ttcaccatgc ctgagaaaag cgttcctgct tcggtcacac cgatgtgaag
     2521 gggataatcg gtcatggctg acataagcct gtatgattct atggtcacag gcacattcga
     2581 cgccttcagt gagattacta tatcgtggaa tcccagatct tccagcatcc tgacgtgccc
     2641 catcgcacta tcagccattg cctgcggggt cgccgctccg tatttcgcca gtatatgctt
     2701 ttccagggac cctgaattta cgcctatcct gatgggtatc cccctggcct tggccgcttc
     2761 cacgactttc ctcacctttt cggtcccgcc gatgttgccg ggatttatcc gtattttgtc
     2821 tgctccgttt tcaatgctcg ccagcgcaag tctatagtcg aaatgtatat cggcaacaag
     2881 gggtatcctg atcgactttc tgattctctt cagcgcttcg gcagcttcac tgtcgggcac
     2941 ggcaactctg acgatatcac agcctgcctc ctcaagcctc tttatttgat cgacggtagc
     3001 agaaacatcc ctcgtatcgg tgttggtcat cgactgtacc gcaacgggcg catcaccgcc
     3061 tatataaata tctccgatac ggattttcct ggttttcttt ctttctatcc tgcccatctc
     3121 cctgtccaaa cctttcaggc agctgtagat cagctgccga tcacatattt ctggaattca
     3181 tttacagcaa tatatatccc gaacaggatg attatgataa aacctgccat cgttatgaca
     3241 gattccttct ccgggttcag gggcttgcct ctgacagcct ctatccctat cagcaggagc
     3301 ctgcctccgt cgagtgccgg gaacggcaga aggttcgtgg caccgagcgc cacgttcagc
     3361 atgcctgtaa tatacatcag gtacaggaac atttgcccta cattcggagc ctggctaaca
     3421 gtggtcgtga tcgtgctcac catcccgata ggccccatca tgtccgaaac gttgaccttc
     3481 ccggttatca gccatttagg gctgtaatat acgctcctga ttatcgaata cgtgaatatg
     3541 gccgactggc caagggcatc cagtacgtct cccctgctaa aaccaaaatc aagtccgacc
     3601 tcgtaccatt cctcggtttt tacctctatc ggcgtcaacg tcgtgacgaa gtggtcaccg
     3661 tttctcaaaa cagtgatctc aagctcattg atgccattgt tctttatcag ctcggtcatt
     3721 ttctcatagg agtccacctc ggtcccgttt atggcaacga ctttgtcgcc cgccatcagg
     3781 cctgcctgtt ccgcgggaga tccggcgtag atctccttga gcacattcga tccttccgga
     3841 tttgtcagat cgagtactac tcccatcatc ggagacgagg tctcaggatg gtatatgggt
     3901 ctcagttctc ctttgagcag tttgccgtcc cttttgtatt ccaccgttac gggctcgcct
     3961 ttcgaaacgt aaaggaactg tactacatcg atcggggcat atatcctctt
//
LOCUS       DAOSCC010000217         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_14195_length_4003_cov_4.483789, whole genome shotgun sequence.
ACCESSION   DAOSCC010000217 DAOSCC010000000
VERSION     DAOSCC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14195_length_4003_cov_4.483789"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PLG72_10010"
     CDS             complement(<1..151)
                     /locus_tag="PLG72_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ81170.1"
                     /translation="MAKNKTVFVCQECGYESSGWLGKCPACMQWNTFVEEKQEAGTRA
                     SAWQPA"
     gene            complement(305..2239)
                     /locus_tag="PLG72_10015"
     CDS             complement(305..2239)
                     /locus_tag="PLG72_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOJ81171.1"
                     /translation="MKSYFNNLITGNSSMLACIDGKGEITRIFWPDIDFPQHVDRFIT
                     GLICPGLWLGTSWLHSSNWKVRQYFIDDTNVAVTEFTHVKYGLKIRQYDFALPDGDVL
                     NRCYEIDNDCGFQLEISFVLYSSSISSVYDTAGVIYDTDLSALIHYRHGYCYGIVSAV
                     PAMQYQLGPGAQESADRGQLSGNDTIGMMKEGSLLWDSVHLGDGDRLRCSVSICFSTE
                     LKSLKKMLRWVKSTDTWEMLDNTAKYWREFLSGARQINTGIPEVDQLYKRSLLVFALM
                     SNKRTGALLAAPELDEQFTRCGRYAYCWGRDAAFITSALDRCGLTEAAEGFYYWAANV
                     QDEDGSWQQRFYTNGDLAPSWGLQIDEGGSIIWGILQHYNATKNMDFLARLWPCVKNG
                     MEFLTRYTDGETGLPWLSFDIWEERLGEHAYSSAAVCAGIEAGARIAELLGESPELAG
                     KWREHAARLREAIFKNFWKPDWNRFIRSVRVKLNSWGEEHTDRKIWLKRNEKSIARDY
                     TLEDGTLDVSLLGLSVPFGIYPADDVHMASTANVLERYLNVNGGLMRYEYDNYIGGNP
                     WILTTLWAALYNIERKDYVKAREYFWWAVRGTTEQGLLPEQIDKLTGKPAWVVPLTWS
                     HAMFVLVLDKLLEAGELHLQ"
     gene            2378..3673
                     /locus_tag="PLG72_10020"
     CDS             2378..3673
                     /locus_tag="PLG72_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ81172.1"
                     /translation="MNEMLQRLVMFVTQIIQVFIFAAGCYFFGVSVYGWRKRKEKPEY
                     AAPFKRFAAVIAAHNEELVIAHIIDSLLEQNYPRELYDIFVIADNCTDNTAAIAASRG
                     ALVYERTDSENRGKGHALEWMFQKIYDMDDKYDAVCVFDADNLVSSNFLYEMNKQLCK
                     GHKVVQGYIDSKNPFDSWITCSYSIAFWLSNRIFQLPRYYLGLSCGLCGTGFCVDMSV
                     LKHIGWGATCLTEDLEFTMKLALNDMKVSWAHDAVVYDEKPITLKQSWRQRIRWMQGH
                     ADCASKYLGPLFRKVFRECNLAAFDCAIYLFQPIRFIFMGLMTVMLWIQSFYPEAPFY
                     TLQYVFPTAVWYVFVILQFLYGPLVILSENKFDFRILLGFLVYPFYCLTWVPITVQGF
                     LTRKNKAWNHTRHTRVITIKELEKPQNALKPSPEASRYQ"
BASE COUNT          974 a         1083 c          946 g         1000 t
ORIGIN      
        1 gggccggctg ccatgccgaa gcgcgggtac cggcttcctg cttctcttcg acgaatgtat
       61 tccactgcat gcatgcaggg catttgccaa gccagcctga cgattcatac ccgcactcct
      121 ggcatacaaa gacggtctta ttctttgcca tagctgaaat tatcctccga aagtctatag
      181 ttgctgaaac tttcaatcca taatatcaaa tataaataca tgcatacatt gtcttatcag
      241 gcagacatgc tgacccgatc cccggcttcc ggccgttgtc agtgcagctt ccgcccgtat
      301 ttcgtcactg cagatgcagt tccccggcct ccagaagttt gtccagcacc aggacaaaca
      361 tcgcatgaga ccaggtaagc gggaccaccc aggcaggctt gcctgtcagc ttgtctatct
      421 gttccggcag gagcccctgc tccgtcgtcc ctctgactgc ccaccagaaa tactcccgtg
      481 cttttacgta atccttcctt tcgatattgt acagtgctgc ccacaatgtg gtcaggatcc
      541 atggattgcc gccgatatag ttgtcatatt cgtaacgcat gagtccgccg ttcacattca
      601 ggtatctttc cagcacattg gccgtggatg ccatatggac atcatcggca gggtatatcc
      661 caaagggaac gctcagcccg agcaggctga cgtcgagtgt tccgtcctca agggtatagt
      721 ctcttgcaat gcttttttca tttctcttaa gccagatctt ccggtctgta tgttcttctc
      781 cccagctatt gagctttaca cgtacgcttc gtatgaacct gttccaatcg ggtttccaga
      841 aattcttaaa gatcgcctcc ctgagcctgg ctgcatgctc gcgccatttt cctgcaagct
      901 ccggggattc gccaagcaat tcggctatcc tggcgcctgc ttcgatgccg gcacacaccg
      961 cggcgctgga atatgcatgt tctcccagcc gctcctccca tatatcgaaa ctcagccatg
     1021 gcaggcctgt ttcgccgtcg gtgtacctgg taaggaactc catgccattt ttgacacacg
     1081 gccagagcct ggcaaggaag tccatgtttt ttgtcgcatt ataatgctga aggatgcccc
     1141 agattatgga gccgccctca tcgatctgca gcccccacga gggtgcgagg tcgccgttcg
     1201 tgtagaatcg ctgctgccag ctgccgtcct catcctgcac attggcagcc cagtagtaaa
     1261 agccctcagc cgcttccgtc agtccgcagc gatccagggc cgaagtaatg aaagccgcat
     1321 cacggcccca gcagtacgca tacctgccgc atctcgtgaa ctgttcatcc aattcaggcg
     1381 ccgccagcag cgccccggtc cgcttgttcg acataagtgc aaaaaccagc agcgaccgtt
     1441 tataaagctg atcgacctca gggatccccg tatttatctg cctggcccct gataaaaatt
     1501 cacgccagta ttttgcggta ttgtccagca tttcccatgt atctgtcgat ttgacccatc
     1561 tgagcatttt cttcaatgat ttcagttcag tcgaaaaaca tatgctcaca gaacacctga
     1621 gcctgtctcc gtctccgaga tgaacgctgt cccacagcaa gctgccttcc ttcatcatac
     1681 ctatcgtatc attgccggac agctgcccgc ggtcggcact ctcctgcgcg ccggggccaa
     1741 gctggtactg cattgcaggc actgctgaaa cgatgccgta gcaataacca tgccgataat
     1801 gtatcagcgc ggaaagatcg gtgtcatata tgacgcctgc tgtatcataa acggaagaaa
     1861 tgctggagga gtatagcaca aagcttattt cgagttggaa tccgcaatca ttgtcgattt
     1921 cgtagcatct gttcagaacg tcgccgtcag gaagagcgaa gtcgtattgc ctgattttta
     1981 aaccgtattt cacatgtgtg aactccgtga cagccacatt cgtatcgtcg atgaaatact
     2041 gcctgacctt ccagttggat gaatgcagcc aggaggtacc aagccacagt ccggggcata
     2101 taaggcctgt tataaatctg tcgacatgtt ggggaaaatc tatgtcgggc cagaagatcc
     2161 tggttatttc tccttttccg tcgatacatg caagcatact ggaattgcct gttatcagat
     2221 tattgaaata cgacttcacc gcatcacccc ccatcgtatc attcacattc tatcataatg
     2281 gcataaaaag cggcaattag tatcaatttg acaggggaat aaatattttc atccgaaagg
     2341 cacaaaataa taaaaaccgc agcgatggag gtctctcatg aatgaaatgc ttcaaagact
     2401 ggtaatgttc gtcacacaaa tcatccaggt ttttattttt gcagcaggct gctatttttt
     2461 tggggtttcc gtgtacgggt ggaggaagag aaaagaaaag cctgaatacg cggccccgtt
     2521 caaaaggttc gccgccgtga tcgcagcgca caacgaagaa ctcgttatag cgcatatcat
     2581 agacagtttg ctggagcaga actatccgcg ggaattgtac gacatcttcg tcatcgccga
     2641 caactgtact gacaatactg ccgccatcgc cgccagccga ggcgcgctgg tatatgagcg
     2701 gaccgacagc gaaaacaggg gcaaaggcca tgcccttgaa tggatgtttc aaaagatata
     2761 cgatatggac gacaaatatg atgccgtctg cgtttttgac gccgataacc tcgtgtcgtc
     2821 gaatttccta tacgaaatga acaaacagct ttgcaaaggc cacaaggtcg tacaaggcta
     2881 catagacagc aagaatccct ttgattcatg gataacgtgc tcctactcca tagctttctg
     2941 gctttccaac cggatattcc agctgccgag gtattacctg gggctcagct gcggcctgtg
     3001 cgggacaggc ttctgcgtgg atatgtcagt tttgaaacac ataggctggg gtgcgacgtg
     3061 cctcaccgaa gacctggagt tcaccatgaa actggccctt aatgacatga aagtatcatg
     3121 ggcgcatgat gccgtcgtat atgatgaaaa gccgataacg ctgaaacagt catggcggca
     3181 gcgcatcagg tggatgcagg gacatgcgga ctgcgcatcg aaatacctgg gcccgctgtt
     3241 caggaaagta ttcagggaat gcaaccttgc tgcttttgac tgcgcgattt acctgtttca
     3301 gccaatcagg ttcattttca tgggcctgat gacggtcatg ctgtggatcc agtcatttta
     3361 cccggaggcg ccgttctaca cgctgcagta cgtgttcccg accgccgtct ggtatgtatt
     3421 cgtcatattg cagttcctgt atggtccgct ggtgatactc agcgaaaaca agttcgactt
     3481 caggatactg ctgggtttcc tggtatatcc attctactgc ctcacctggg tacccataac
     3541 ggtccagggt ttcctcacga gaaaaaacaa agcgtggaac catacgagac acacccgcgt
     3601 gatcacgata aaagaactgg aaaagcccca aaatgccctg aaaccttccc ccgaagcctc
     3661 ccgctaccaa taggggacag tccttcattt tcggggacca ttttcgggga cggttcttca
     3721 gttaaggaca gtctcaactt ggggacagtt cttcatttat cttaaataga agacagtttt
     3781 catcttcagg gacagtcttc tggttcgggg acagttcttc agttcccgga aaggggatcc
     3841 gccttcgggg acggtccttc tgttcgggga cagttctcaa cttttgaaac caacagggca
     3901 accccagaca tgaaatgcag gactgtctct taacaaagaa gcgttccatc aaaaagtgca
     3961 gaactgtccc ctgaactgga gaactgtccc tcatccaaaa atg
//
LOCUS       DAOSCC010000218         3950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_14455_length_3950_cov_3.176380, whole genome shotgun sequence.
ACCESSION   DAOSCC010000218 DAOSCC010000000
VERSION     DAOSCC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14455_length_3950_cov_3.176380"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1236
                     /gene="galT"
                     /locus_tag="PLG72_10025"
     CDS             259..1236
                     /gene="galT"
                     /locus_tag="PLG72_10025"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOJ81173.1"
                     /translation="MAELRWHPLIRDWVMIASHRQDRPQMPKDWCPFCPGSGRVPDDY
                     EVYRYDNDFPALMLQPPKPDDVATDFYKTAEAYGKCEVILFSPDHSASLHQFSVEHIV
                     KIVDLWTERFAELSKDERIKYIFIFENRGEMVGVTMPHPHGQIYGYPFIPKKIQLELE
                     SCREHYENNGTCLICDMMKDEMKHGDRVIFENEDFVVFLPFFTEYPYGVYIVSKNHKQ
                     NLTQFDGREKANLARALKETTGMLDSLFGFPFPYMMCMHQDPVNSGDVSDYYHFHIEF
                     FPPMRSSNKQKFNASSETGAWAHANPTKPEEKAAELREAHKRFLGSQGT"
     gene            1352..2542
                     /locus_tag="PLG72_10030"
     CDS             1352..2542
                     /locus_tag="PLG72_10030"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ81174.1"
                     /translation="MTIADLKKKFIEVYGGTEEGIRVFSSPGRVNLIGEHTDYNGGYV
                     FPAALTLSSTVVARPRDDRMMNLIATDLGIQVTGSLDRLDEYKSLKWGNYQYGVADEL
                     QKAGYRLVGCDLLYHDTVPLGAGLSSSAAIEVATAVTLVSLGNAAYDLDQPIDMIEMA
                     LICQKAENNYVGVNCGIMDQFASAMGKKDHAIFLNCRDLSYKHVPLNLKGYKIVIANT
                     NKKRGLADSKYNERRSQCEAAFEILKKFLPGATCLGEISMDDFNRFRHEIKDEVIRKR
                     AEHVIAEDDRVLKSVEALNNDDIALFGKYMIASHDSLRDLYEVTGAELDALVEEALKV
                     EGVAGSRMTGAGFGGCTVSIVREDAVDRFIEEVGKGYTARTGLVPTFYISEVGDGGRE
                     LSRS"
     gene            complement(2734..>3950)
                     /locus_tag="PLG72_10035"
     CDS             complement(2734..>3950)
                     /locus_tag="PLG72_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HOJ81175.1"
                     /translation="RISLRRFIGMSFRDRYRKTFSQYYDHSLDDDVILRMIDQKTRSH
                     KKRPRYRWAAIALASVLLVLSTTYVFAAVGLLDLAGIFRANDPVSAKLADSGIVQELG
                     IVGEDNEFTLKLVAFTGDIETQKVLFELIPKKDLSYIDDYEIRLTAQTSSPQIMEETG
                     RGWGYWPVEAPGTKVISDDGSEVYYFNYELPEFWVKGTTGDVTIRVLGVNLYYKGQPV
                     GMIACELFYTFTPDRSILQEPVVVAVNKLLTKDVIDDFVSYPYDEDYEYYKGKITAPV
                     KRRSLMIDNITFSNYKAEINARIMEDDIHLLEACSIWDQFTKPTFATKHFLNGIDDRN
                     AYKMTIVDNTERLRLFVDGAERPVLKEMYTALPGRGDDGYFSPSIRFEGFDYENAKKV
                     EIRFGDEVITIK"
BASE COUNT         1038 a          949 c         1039 g          924 t
ORIGIN      
        1 gtttcgatcc gctgacgtcg gcggtactga gccttgcgga aatcaaacag atggttgacg
       61 agatgttcga ggcaaacagg aactggctgc ctcagttcaa acattttaaa tagatttcag
      121 gttcctggta cgacatgtgg tcatgaggat aattgccaga acatgggatg gttcccgtgc
      181 actggcggtt tggacacggg aaccgttccc gtgtcttgcc agtcgtgccg ggtactctgt
      241 attggaggag gatcaaaaat ggcagaatta cgatggcacc ccctgatcag ggactgggtc
      301 atgatcgcgt cacacaggca ggacaggccc cagatgccca aggactggtg tccgttttgc
      361 cctggttcgg gaagggtccc ggatgattat gaagtataca ggtatgacaa tgactttcct
      421 gccctgatgc tgcagccgcc caagcctgat gatgttgcaa ctgattttta caaaacggct
      481 gaggcctacg gcaagtgcga ggtcatactt ttttctccgg accattccgc atcgctgcat
      541 cagttttcgg tggagcatat agtaaagatt gttgacttgt ggacggaacg cttcgccgaa
      601 ctctcgaagg acgagaggat caaatacatc ttcattttcg aaaacagggg agaaatggtt
      661 ggagtcacga tgccccaccc tcatggacag atctacggat atccgttcat tcccaagaag
      721 atccagctcg aactggagtc atgcagggaa cattatgaaa acaatggcac atgccttatc
      781 tgtgatatga tgaaagatga gatgaaacac ggcgacaggg tcatttttga aaatgaggac
      841 tttgtggtct tcctgccatt ctttactgaa tacccatacg gcgtgtatat agtgagcaag
      901 aaccacaaac agaatctcac ccagttcgat ggaagggaga aggcgaacct ggcacgggct
      961 ctgaaggaaa cgacagggat gctggactcg ctttttggat tcccgttccc gtatatgatg
     1021 tgcatgcacc aggatcccgt aaacagcggc gatgtgagcg attattacca tttccatatc
     1081 gaatttttcc cgccgatgcg ctcatcgaac aagcagaagt tcaatgcctc cagcgagacc
     1141 ggggcatggg cccatgccaa tccgacgaaa cctgaggaga aggcggcgga actcagggag
     1201 gcgcacaaga ggttcctggg cagccaggga acataaaaag gatgcagcga tccgggacag
     1261 ccggcagcaa catgcgctga aaagcgagtg atgcgaaacg ttacatggaa tgtcatcgaa
     1321 acttggataa aatccggaaa gaggtgtcga tatgaccatt gcggatctta aaaagaagtt
     1381 catcgaagta tacggcggta ccgaagaagg cataagggta ttctcatcgc cggggcgtgt
     1441 caatctgatc ggcgagcaca ccgattacaa cggaggatat gttttcccgg ccgcattgac
     1501 gctgagttcc accgtcgtcg ccagaccgcg ggacgacagg atgatgaacc tgatagccac
     1561 cgatctgggc atacaggtaa cagggtcgct tgacaggctg gatgagtaca aatcattgaa
     1621 atggggcaac taccagtacg gcgtagccga tgaacttcaa aaggcagggt acaggctggt
     1681 tggctgcgac ctgctgtatc acgacactgt gccgctggga gccggccttt catcatccgc
     1741 ggcgatagaa gtagcgacag ctgtaacgct ggtatcattg ggcaatgcgg cttacgacct
     1801 ggaccagccc atcgacatga tcgaaatggc gctgatatgc caaaaggctg agaacaatta
     1861 tgtcggtgtc aattgcggca taatggacca gttcgcatcc gcgatgggaa agaaagacca
     1921 cgccatattc ctcaactgca gggacctgag ttacaaacac gtgccattga acctgaaagg
     1981 gtacaaaatc gtgatcgcca acacgaacaa gaagagggga cttgccgact ccaagtacaa
     2041 tgagaggaga agccagtgcg aagcagcttt cgaaatactg aaaaaattcc tccctggagc
     2101 tacatgcctc ggcgagatat cgatggacga tttcaacagg ttcagacatg agataaagga
     2161 tgaagtgatc cggaaaaggg ctgagcatgt gatagctgag gacgacaggg tcctgaagtc
     2221 ggtcgaagcg ctgaacaatg acgatatagc gctgttcggc aagtacatga tcgcgtccca
     2281 cgattcgctg agagacctgt acgaggtaac aggcgctgag cttgacgccc tggtggaaga
     2341 ggcactgaag gtggaaggcg ttgcaggctc aagaatgacc ggtgcaggct ttggaggctg
     2401 tactgtcagc atagtaaggg aagatgccgt cgacaggttc atcgaagagg taggcaaagg
     2461 atacacggcc aggaccggac tggtgccgac tttctacatt tcggaggttg gcgacggcgg
     2521 cagggaattg agcagatcct gaagtcactt aaagcaagtt ggaatagatc taaagcatgt
     2581 aaatcagatc cttaagggtg cggatccccg gaggatatac cattgacagt gaagtcacct
     2641 ggtcacaccc gtcgatttcc atgaaaaggg atctcccgct gcagaacgca gagcgctcca
     2701 agcgggagat cccaatctgt tacggtaaat tcatcactta attgtgatca cttcatctcc
     2761 aaaacgtatt tctacttttt tcgcattctc atagtcgaat ccttcaaatc tgatcgaggg
     2821 actgaagtat ccgtcatctc ctctgcctgg taaagcagta tacatttctt tcagcacagg
     2881 tctctctgca ccgtcgacaa acagtcggag acgctctgta ttatcgacta ttgtcatttt
     2941 gtatgcattc ctgtcatcta tgccattcag gaagtgtttt gtggcaaatg tgggcttggt
     3001 gaattgatcc catatgctgc acgcttccaa taagtggatg tcatcttcca ttatccttgc
     3061 attgatttca gccttgtagt tggagaatgt aatgttgtct atcattagcg acctgcgctt
     3121 taccggcgct gtgattttgc ctttataata ctcatagtct tcgtcatacg ggtatgaaac
     3181 gaaatcatca ataacatctt tagtgagcag cttatttacc gccacaacaa caggttcctg
     3241 caggatcgaa cggtcaggcg tgaatgtata gaacaactcg caggctatca taccgacagg
     3301 ctggccctta tagtacaggt tgacgcccag gacgcgtatc gtcacatctc ctgtggtgcc
     3361 tttcacccaa aattcaggaa gctcgtagtt gaaatagtac acttcgctgc cgtcatcaga
     3421 gatcactttc gtgcccggcg cttctaccgg ccaatagccc cagccccggc ctgtttcttc
     3481 cattatctgc ggggaagatg tctgcgcggt gagccggatc tcataatcgt cgatatagct
     3541 gaggtctttc tttggtatca gctcgaacag caccttctga gtctcgatat cacctgtgaa
     3601 tgccaccagt ttcagggtga attcattatc ctcgccgaca atgccgagct cctgcacgat
     3661 ccccgaatcc gcaagctttg cactgaccgg gtcatttgcg cggaagatgc cggccagatc
     3721 aagcaacccg acagcggcga acacataagt cgtactcagt accaggagca ccgaagcaag
     3781 cgcaatggca gcccacctgt acctggggcg ttttttatgg ctgcgggttt tttgatcgat
     3841 catacgtaaa attacatcat cgtccaggga atgatcataa tattgactga aagttttcct
     3901 gtacctgtcc ctgaagctca tccctataaa cctccttaag cttattcttc
//
LOCUS       DAOSCC010000219         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_14606_length_3921_cov_4.688826, whole genome shotgun sequence.
ACCESSION   DAOSCC010000219 DAOSCC010000000
VERSION     DAOSCC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14606_length_3921_cov_4.688826"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLG72_10040"
     CDS             <1..209
                     /locus_tag="PLG72_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81176.1"
                     /translation="IASFIRNQMETILLGKPVDRFDDITSYIDNVRKMDAGLSDKIDK
                     AFRLRMPAEARPVHEADRGKSISA"
     gene            297..875
                     /locus_tag="PLG72_10045"
     CDS             297..875
                     /locus_tag="PLG72_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ81177.1"
                     /translation="MSIERNWTVLFIGGPSGSGKSSLAYQIARLYGVNVLEADDVYLS
                     VKAVTKKEDFPAIHYWDTGADWKSIGVEGNVKWLTDVSKEMVPVLEALMNRHLEDKLP
                     VIVEGDFIYPGLMASLNNPEVKSIFLIEPDVDQIMQNYQSREGGGLQRYRAEISVAYG
                     KWIKDTCKKNNISVIEARPWDTVLVRAINSLS"
     gene            1150..1377
                     /locus_tag="PLG72_10050"
     CDS             1150..1377
                     /locus_tag="PLG72_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81178.1"
                     /translation="MFEFFNKPEYGPDGERRRIFCYYDNEKTGKKDFWCKVHAEIKDE
                     SEIKDDRLQFEGKIISYTADHRNPYKTDMIY"
     gene            1516..1893
                     /locus_tag="PLG72_10055"
     CDS             1516..1893
                     /locus_tag="PLG72_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ81179.1"
                     /translation="MKDMKKLPDAEFEVMKIVWQNDPPVTANIVMEQLGKKKKWKTQT
                     VISLMFRLVERGFLRTEKAGRERLYYPVVSREEYLKFETGNFIKQYHDNSLLNLVNTL
                     YDDDALTDRDISELLQWIKERRG"
     gene            1895..3604
                     /locus_tag="PLG72_10060"
     CDS             1895..3604
                     /locus_tag="PLG72_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOJ81180.1"
                     /translation="MKDFMIMLTTHSAALSAVILLYAAVTPVLSKRYSAKWLYYSWLI
                     IVIGLIVPVWPQFEVPGINVTAPAEAVLTRPLMAVPATVADTATRVSNAVQLNHPVQS
                     GNFVMQHAITSIQWWQAAAAIWATGAVAFLLGRCFRHYRLMKTLGRWSEEVKDEQVLA
                     LMNDIKKELGIPGRVGLHLCPYTGSPMIAGLFRPRILLPVTDIPKEELRLILKHELIH
                     CRRKDTWYKCLLLLATAMHWFNPAVYLMAKVINAQCELSCDAEVLRDSEPDMRYRYCK
                     AIVGLLETRLKTRTALSNNFYGGKSGMKIRISSILDARKKKAGLAVACAMLMLTAAIG
                     IASAAIRVDNGINDMSGNVNADVVVRLADHNKTPENYDTSACYVDARPELEFYIDGRD
                     IAQIEVTCRNEYLYAVDLTHTQHEKYWNAEYYQTYDDEIGTSTFYPERLYGKTIKFSF
                     DKEFSDYGEIWYRWTAENLYRWASEDNFSHFVGFGVDPKIELPEDMTEEQKLKLAAGE
                     DGSGAANIGHICLDGYPEELASDRIEIKVTDREGNSVTKYINVEISNNELMQTVVTAN
                     VED"
     gene            3727..3915
                     /locus_tag="PLG72_10065"
     CDS             3727..3915
                     /locus_tag="PLG72_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81181.1"
                     /translation="MNYYERIQKSIDYIESRLKDNIEIEKAAAESYMSSSTVPRQPCH
                     RNSHPWQEPSLHLDKKSK"
BASE COUNT         1169 a          806 c         1008 g          938 t
ORIGIN      
        1 ctattgccag cttcatcaga aaccagatgg aaactatcct gctcggcaaa cctgtagata
       61 ggttcgatga tatcacgtca tacatcgaca atgtcagaaa gatggatgcc ggattatccg
      121 ataagatcga taaggctttc cgcctgagga tgcctgcgga agcaaggccg gttcatgagg
      181 cagacagggg caaaagcata tctgcctgag catatctgtg ataaaataag gcctgtggca
      241 taataccggc ttaatcgtca atgtcaatag tgatttggta taaggatggg caatttatga
      301 gtattgaaag aaactggaca gttttgttta ttggaggtcc ttcagggtcg ggaaaatcga
      361 gcctggctta tcagattgcc aggctttatg gcgtaaatgt gctggaagca gatgatgttt
      421 acctgtctgt gaaggcggtc acaaaaaagg aggattttcc cgcaatacat tactgggata
      481 caggtgcgga ctggaaaagt atcggagttg agggcaatgt aaaatggctg accgatgtca
      541 gcaaagagat ggttccggtg cttgaagcac ttatgaacag gcatcttgaa gacaagctgc
      601 ctgtcattgt cgagggtgat ttcatctatc ccgggttaat ggcatctctc aacaatccgg
      661 aagtgaaatc aatttttttg atagaacctg atgttgatca aataatgcaa aattaccagt
      721 caagagaagg cggcggttta cagcggtaca gggctgagat aagtgttgca tacggcaaat
      781 ggatcaaaga tacctgcaaa aagaacaaca tcagcgtgat cgaggcaaga ccctgggata
      841 ctgtccttgt gagagctata aatagtttat catgacatca cggagtgacg tgcttgctcc
      901 tgtaagacgt cgcaatacac tatgtgtacc ttcttttagt tcctgaccat ccatggtaga
      961 atgtagtaaa actgtatctg cgttgtacag cctggatcaa attacactta cacagggagg
     1021 ttccggatgg gaaggagtaa accaggaaag ctccttgggt acatgtgaaa tcacccgcca
     1081 ggttatcgca gaaaaagcat aactgtttat tgccgggaca aagacacggc agagacatga
     1141 gcgagacata tgtttgagtt cttcaataag ccggaatatg gtccggacgg tgagagacgc
     1201 aggattttct gctattacga taacgagaag acaggcaaaa aagatttctg gtgcaaggtg
     1261 catgcagaaa tcaaagatga gtcggagatc aaggatgaca ggcttcagtt tgagggtaaa
     1321 atcatctcat acactgctga ccatagaaat ccatataaga ctgacatgat ttattaaaaa
     1381 cccgggaggc cgcagcggtt acggtctctt tttattggca gggatacatg tttcatgcaa
     1441 aaaatacttg acattcattt tagacaattg tagaatatgt atagtagaca attgtcttaa
     1501 atggggggtg atgcgatgaa agacatgaaa aaattgccag atgcggagtt cgaggtaatg
     1561 aagatcgtgt ggcaaaacga tccacctgtt actgcgaaca ttgttatgga gcagctgggg
     1621 aagaaaaaga agtggaaaac ccaaacggtg atctctctca tgttccggtt ggtcgagcgg
     1681 gggttccttc gtactgaaaa agcgggcaga gagcgtcttt actatcccgt tgtcagcagg
     1741 gaagagtatc tgaaattcga aacaggtaat tttataaagc aataccacga caactccctg
     1801 cttaaccttg tcaatacgct gtatgatgac gatgcactga cagacaggga tatcagcgaa
     1861 ctgctccaat ggataaaaga acggaggggc tgaaatgaag gacttcatga taatgcttac
     1921 gactcattcc gcagccttat cagccgttat tcttttgtat gcagcggtaa ccccggtatt
     1981 gtcaaagcgt tactctgcca aatggcttta ttattcatgg ctgataattg ttataggatt
     2041 gatcgttccg gtctggccgc agtttgaggt gccaggcata aatgtaacgg cgccggccga
     2101 ggctgtattg accaggccgc ttatggcagt tccggcgaca gttgcagaca ctgccacccg
     2161 ggtcagcaat gctgttcaac ttaaccatcc cgttcaatcc ggcaatttcg tgatgcagca
     2221 cgccattaca agcatccagt ggtggcaggc agcagcggct atttgggcaa caggagcggt
     2281 cgctttcctt ctcggccggt gtttcagaca ttatcggttg atgaaaacgc tcggacgctg
     2341 gagcgaggag gttaaagatg aacaggtatt ggcgcttatg aatgatataa aaaaagaact
     2401 ggggatacca gggcgggtag gcctgcattt atgcccatat acaggcagtc ccatgattgc
     2461 gggcctcttc agaccgcgga tattgctgcc ggtgacagac attccgaagg aagaactccg
     2521 cttgatcctg aaacatgaac tcattcactg cagaagaaag gatacatggt ataagtgcct
     2581 gctgctgctc gcgacagcca tgcattggtt caatcccgcc gtttacctga tggcaaaggt
     2641 gatcaatgca cagtgtgaac tttcatgcga tgccgaggta ttacgcgact ctgaaccgga
     2701 tatgcgctac aggtactgca aagcgatcgt cggcctgctt gagacccggt taaaaacaag
     2761 aacagcgtta tcaaataatt tttatggagg taagagtggt atgaaaataa ggatttcgtc
     2821 catcctggac gcaaggaaaa agaaagccgg tctggctgtt gcctgcgcca tgctgatgct
     2881 gacggccgca atcggaattg caagcgcagc gataagagtg gataatggaa taaatgacat
     2941 gtcaggaaat gtaaatgccg atgtagttgt ccgtctggcg gatcataata aaacaccgga
     3001 aaattatgac acctcagcat gttatgtgga tgcaagacct gagctggagt tttacattga
     3061 cggaagagat atagcccaaa tagaggtaac atgccggaat gagtacctat acgcagttga
     3121 cctgactcac acacagcatg aaaaatactg gaatgccgaa tattatcaga catacgacga
     3181 tgaaatcggg accagtacat tctatcctga gcgcctttat ggcaaaacaa taaagttctc
     3241 attcgacaaa gagtttagtg actacgggga gatttggtac aggtggacag ccgagaacct
     3301 gtatagatgg gcaagtgaag ataatttctc ccatttcgtc ggtttcggcg tcgatccgaa
     3361 aatagagttg ccggaagata tgactgagga gcagaaactt aagctggcgg caggagaaga
     3421 cggttccggc gccgcaaaca tcgggcatat ctgccttgac ggctatcctg aagaactggc
     3481 cagtgaccgt atagaaataa aggtaactga cagggaaggc aattcggtga caaagtatat
     3541 caatgttgaa ataagcaaca acgaactaat gcaaaccgta gtcactgcga acgtggagga
     3601 ttaaaccagt tggtccatga acggtcttga aatcgtgttt ctgtcgaaat tcaattcctt
     3661 ggctacgcct tatgtcgata ccctgccgac ccctccgccg aaagtgtcgc tacaggagta
     3721 ctccggatga actactacga acgtattcag aaatccatcg actacattga aagcaggctc
     3781 aaagacaata ttgaaatcga aaaagcggca gcggaatctt acatgtccag ttcgaccgtc
     3841 ccccggcaac cttgtcacag gaatagccac ccctggcaag aaccgtcctt gcatcttgat
     3901 aaaaaaagta aataaaggta g
//
LOCUS       DAOSCC010000220         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15319_length_3788_cov_4.872489, whole genome shotgun sequence.
ACCESSION   DAOSCC010000220 DAOSCC010000000
VERSION     DAOSCC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_3788_cov_4.872489"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2315
                     /locus_tag="PLG72_10070"
     CDS             <1..2315
                     /locus_tag="PLG72_10070"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ81182.1"
                     /translation="GEKAFGATADMPDMFQMVVDNGVVTKMLSGEQALPIPENGFIVV
                     TRSEGAKKLKSVFSVGDIVRMDITTTPDWQQLKMSISGSAILVDNGVIPEKFSFEASD
                     IKVKSPKTALGISKDGKTLYFVTVDGRQTASLGLTLKEMAQFMQSIGAYNAINMDGGG
                     STTMVARPLGETGVKVMNKPSDGVVRTVINGIGVFTSAPAAELAGLIIETDDRYMFTN
                     ATRAFRVKGYDKYLNPVEVDMSQVEWSVSGVKGTFEGNVFRPSTYGEGTVKARIGDIS
                     TSIRISVLSKPVTLTLDTKSLKLPVGKSKTFKVIGTNPRGYKATINPEDLNWKVSSDF
                     GSFSNGVFKAEKKGAGYIEVSFGDAYAYCPVSVSAETREVVERFEEVKGFFQSYPETI
                     EGYYTVTDEQKVSGKSSGKLVYNFSTNTDVTRAAYWVLPEKGYVLDADTHKIGLNVYN
                     DHENSGWLRAELVDADGKKQVVDFAKIMNWVGWNYVEASVESIKKPARLNRIYIVQVN
                     PDMDAGTLYFDDLTFVTNGYPSLDGIEVPKDTPFVDEANKAVSFNKATDDSFRFGVMG
                     QSRAPENDVEKRLTKVFADKVTKYLEVGAVVGSGSHESITANVKKKPVVATHSVDLKS
                     TKATDYAYSVTDFKNSRFIKLDTRKNSLRLSDPDQWSKFLNDLESFKGKNVFIFMESS
                     PENFTDKLELALFKKTLKDYRFDTLKNVCVFYKGSENKCTLEDGVRYFETAGYEVSGV
                     KSKDTSKAQYILVTVKGSEVTYVYKPIESS"
     gene            2618..3700
                     /locus_tag="PLG72_10075"
     CDS             2618..3700
                     /locus_tag="PLG72_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ81183.1"
                     /translation="MPAEILFSSVEYDKYDADVTLPAKFGRLIDRMGMQDVVRDKWTA
                     IKMHLGRYIGYSTIPPIFVKILVDKLKSYGAKVYITDQIVSEARARGYNEDYLGAPIV
                     AAAGVTGRYFYEKHVDFKSLKNIDIAGNIHDADVMIDLSHVKGHGVCGYGGACKNIAM
                     GCVTDRTRHQIHGLEGGIEWDENLCIHCGLCVNSCNHYANRFNEDGKYEVNYHHCTLC
                     QHCVKVCPASAMRMLDNQYEDFQKGMALCTSEVLKTFEPGHVFYINFLINITALCDCW
                     GLTTPSLVPDIGVMASTDIVAIEKASLDSIKTENLIPAGVPKGHELGTKGHLFERLHG
                     KDPFVQLRELEKLGLGTMEYETETID"
BASE COUNT         1129 a          859 c          997 g          803 t
ORIGIN      
        1 ggggcgaaaa agctttcggc gcgaccgccg acatgccgga catgttccag atggtcgtgg
       61 acaacggagt cgtaacgaag atgctgtcag gcgaacaggc cctgccgata cctgaaaatg
      121 gttttatcgt cgttacccgc agcgagggag caaaaaagct taaaagtgtt ttcagcgttg
      181 gagacattgt ccgcatggat ataactacga ccccggactg gcaacagctt aaaatgtcca
      241 tttccggcag tgccatcctg gtcgataacg gggtcatccc ggagaaattc tctttcgaag
      301 cgtcggatat taaggtaaaa agcccaaaaa cagcacttgg catatccaaa gacggtaaaa
      361 cactgtattt cgtcacggtg gacggaagac agacagcgag cctcggactg acattgaagg
      421 aaatggccca atttatgcag agcattggcg catataacgc gataaatatg gacggaggcg
      481 gctctaccac catggtggca agacccctgg gagaaacagg cgtcaaagtc atgaacaaac
      541 cttccgacgg tgtcgtcagg acagtcatca acggaatagg ggtgttcacg tcagcgcctg
      601 ctgcggagct tgcaggcctg atcatcgaaa ccgacgacag gtacatgttt acaaatgcca
      661 cccgggcatt cagggtaaag ggctatgata agtatctcaa cccggtcgaa gtggatatgt
      721 cccaggtcga atggagcgtt tcgggagtaa aggggacatt cgagggcaac gtattcaggc
      781 caagtacata cggggaaggg acagtaaagg cacgtatcgg cgatatcagc acgtcaataa
      841 ggatcagcgt gctttcgaag cctgtaacgc tcaccctgga cacaaagagc ctcaaactgc
      901 cggtaggaaa gtctaagact ttcaaggtga tcggaacgaa ccccaggggt tataaagcga
      961 ccataaatcc ggaagatctt aactggaaag tcagcagtga tttcgggtca ttcagcaacg
     1021 gtgtattcaa agcggagaaa aagggcgcgg gatatatcga ggtctctttc ggcgatgcct
     1081 acgcctactg cccggtttca gtatcggcag aaaccaggga ggtagtggag cggtttgaag
     1141 aagtaaaagg atttttccag tcgtaccctg aaaccataga aggatactat acggtcacag
     1201 atgaacagaa agtttccgga aaatcctccg gcaagctggt gtacaacttt tcgacaaata
     1261 ccgatgtgac ccgtgcagca tattgggtcc tgcctgaaaa aggttatgtc ctcgatgccg
     1321 atacgcataa gatcgggctc aatgtctaca acgaccacga gaactccgga tggctgcgcg
     1381 ctgaactggt tgatgcggac ggcaagaaac aggtggtgga tttcgccaaa atcatgaact
     1441 gggtcggctg gaattatgtc gaggcatccg tggaaagcat caaaaagccg gcaaggctta
     1501 ataggatcta tatagtgcag gtcaatccgg acatggacgc aggcaccctg tattttgacg
     1561 accttacctt tgtaaccaac ggttacccgt cgctggacgg catagaagta cccaaggata
     1621 cgccgtttgt cgacgaagcg aacaaggctg tgagcttcaa taaagcaacc gacgattctt
     1681 tcaggttcgg tgtaatggga cagtcgagag ctccggagaa tgatgttgaa aaaaggctca
     1741 caaaagtatt cgcggacaag gtgaccaaat acctggaggt cggagcggtc gtcggaagcg
     1801 gctctcatga gtcgatcacg gcaaatgtta aaaagaagcc tgtagtcgcg acacacagcg
     1861 ttgacctgaa gagtaccaag gcaacagatt atgcgtactc ggtcacggat tttaaaaaca
     1921 gcaggttcat caagctcgat acgaggaaaa acagcctgag gctgagcgat ccggatcaat
     1981 ggagcaaatt cctcaatgat ctggaatcct tcaagggcaa gaatgtattc atatttatgg
     2041 agagttctcc ggaaaacttt acagataaac ttgaacttgc cctgttcaag aagacactga
     2101 aggattaccg tttcgataca ctgaagaatg tatgcgtatt ctataaaggc agcgaaaaca
     2161 aatgcacgct ggaagacgga gtgaggtatt tcgaaacagc cggctatgaa gtatcaggcg
     2221 taaagtcgaa agacaccagc aaagcccagt atatactggt aacggtcaaa ggctctgaag
     2281 tgacgtatgt atacaaaccg atcgaatcat cctgataatg tcctgctgat actgtcaccg
     2341 gaaacccggc acagtccggc aagacgcaga catgataccc atgtgcagtg caaaagaaaa
     2401 ggacttgctg cataatggct cacagcataa aagcctgcag tacaggcaga aaaatttcag
     2461 ataccgcaat aaagaaggcg caaaatgggt aatactttaa agcatcagat catcgggggg
     2521 tcatttcccc tttgttctga tgcttagtat tgtctgtttg cgtttttcat tatttaaagc
     2581 aacaacccca ttcaattaaa aaggaggact ttataaaatg cctgctgaaa tactgttttc
     2641 atctgtcgaa tatgacaaat acgatgctga tgtgacactg cctgccaaat tcggacgcct
     2701 catcgaccgt atgggaatgc aggatgtggt cagggataag tggaccgcga tcaaaatgca
     2761 cctgggaaga tatatcggct actccacgat accgccgatc tttgtgaaaa tactcgtgga
     2821 caagctgaag agctacggag ctaaagttta cataactgat cagatcgtat ccgaagccag
     2881 ggcgcgcgga tataacgaag attatctcgg ggctccgata gtcgcggctg caggagtcac
     2941 gggcagatat ttctatgaaa agcatgttga tttcaaaagc ctgaaaaaca ttgatattgc
     3001 aggcaacata cacgacgccg atgtcatgat cgatctgtcg catgtgaaag gccacggggt
     3061 ctgcggatac ggcggagcct gcaagaacat cgcaatgggc tgtgtcacgg acaggacgcg
     3121 ccaccagata catgggctgg aaggcggcat cgaatgggat gagaatttgt gtatccactg
     3181 cgggctttgc gtcaacagct gcaaccatta tgccaacagg ttcaacgaag acggcaaata
     3241 cgaggtaaac taccaccact gtacactgtg ccagcactgc gtaaaggtgt gtccggcatc
     3301 agccatgagg atgctggata accagtatga agatttccaa aagggcatgg ccctgtgcac
     3361 tagtgaggta ctgaaaacat tcgagcccgg ccatgttttc tatataaact tcctcatcaa
     3421 cataacggcg ctgtgcgatt gctgggggct gaccacacca tccctcgtcc cggatatagg
     3481 agtcatggca tcaacggata tcgtggccat tgagaaggcc agccttgatt caataaaaac
     3541 agaaaacctg attcctgctg gagtcccgaa aggccatgaa ctcggaacga aaggccacct
     3601 gtttgaaagg ctccatggga aagatccatt cgttcagctc agagaactgg aaaaactggg
     3661 actcggcacg atggagtacg aaactgaaac catcgattag tgccggcagt gccggaaata
     3721 cagcaataga aacaattaga gcagggaagc cgatcaacat cacttccctg cttctgcata
     3781 tatgtgcc
//
LOCUS       DAOSCC010000221         3771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15409_length_3771_cov_5.397470, whole genome shotgun sequence.
ACCESSION   DAOSCC010000221 DAOSCC010000000
VERSION     DAOSCC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15409_length_3771_cov_5.397470"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLG72_10080"
     CDS             complement(<1..1038)
                     /locus_tag="PLG72_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ81184.1"
                     /translation="MLIFNKKTNTLEQSLYRYELQDVRTPNLYREIYSYDEIPKCAFN
                     HRRVPMFPADEIWITDTTFRDGQQSREPYTVEQIVTLYKMLHRLGGPKGIIRQCEFFL
                     YSDRDKEAVYKCQELGYEYPEVTSWIRATKSDFQLAKDMGVKESGILVSCSDYHIFKK
                     LNMTRKQALDHYIGIIKSAIEVGIRPRCHFEDITRADFYGFVVPFALELRKLSEESGV
                     PIKIRACDTLGYGVPYPGVALPRSVPGIIYGLRHHAGFPSELIEWHGHNDFYKAVVNS
                     ASAWLYGASSVNCSLLGIGERTGNTPLEAMVIEYAQLRGTTDGMDTTVITEIAEYYEK
                     ELGYEIPSRTPF"
     gene            1209..2489
                     /locus_tag="PLG72_10085"
     CDS             1209..2489
                     /locus_tag="PLG72_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ81185.1"
                     /translation="MAVKKKEKAKSIAEQVSSQGKIYLLTSVAVGLVMSAVYVLITGR
                     YAEALLVLATAIITSQVMRIANGIMIRRISRKFEEELNVIKQGDYSRFLDPNSFGVLS
                     GIGSSLNSVLSDIRSLIDSFFSLSHSIIQASRKVGSTAEQAVAAITEISKTVDEIAKG
                     ASDQAVDAWEGVQLVDKLSEQIDFVYKSYSDVMDETNNIHRLNEAGLESVQILRERSE
                     QSYNSTEKIFSVVENLTNTTKDIGAFVQSIEDIAEQTNMLALNAAIEAARAGEAGKGF
                     AVVAEEVRQLADQSRRFTEEIINLMDNINVESQQAIQAMEMMRKVSKEQNEAVDSTNK
                     AFGNIAEGIVVIVDKIKAVNDAVLAMQKGKDNVISAIENISSVSEETAAASEQVAATT
                     EQQLKEIDEMKVAAEQLDELVQELDKKLKKFKIK"
     gene            complement(2555..3286)
                     /locus_tag="PLG72_10090"
     CDS             complement(2555..3286)
                     /locus_tag="PLG72_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ81186.1"
                     /translation="MKLTVLGNNGPYPAAGSACSGYLLQSGTTNVLIDCGNGVLSNMQ
                     KIISIDKLDAVILTHLHGDHVSDMHVLKYAIQLRRKRGLYDKLLRVYAPAEPSEEYER
                     LDVKDAFELGAITDDTQLAIGDIKFSFLRMKHPYTDYAVSAECAGKKFVYSGDTSWTD
                     GLVTFAKGADLLMLDAGFLEKDWAEGAPHMTAAQCGKAAAMAGAKKLLLTHIWPDYDL
                     KDVAEEAAANFPNAEAAMVMKEYEI"
     gene            complement(3488..>3771)
                     /locus_tag="PLG72_10095"
     CDS             complement(3488..>3771)
                     /locus_tag="PLG72_10095"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ81187.1"
                     /translation="GLAVGLLTSKIRKSGALKQGRLIISTVAGAAVMIVGYFLAEAFL
                     LGMFDEAFGLTAAVAELLPNTLQGIFSAVLGYILVLVFSKINLDRPAGR"
BASE COUNT          931 a          979 c          954 g          907 t
ORIGIN      
        1 gaacggggtc ctagagggta tctcgtatcc aagttccttc tcgtaatact ctgctatctc
       61 tgtaataacg gtagtgtcca taccgtcagt ggtgcccctg agctgcgcat attcgatgac
      121 cattgcctca aggggcgtat tgcccgtcct ctcgccgatg ccgagcagtg aacagttgac
      181 tgacgaagcg ccgtacagcc atgcggaagc tgaattcacg accgccttgt agaagtcgtt
      241 atgcccgtgc cattcgatca gttcgcttgg gaatcccgca tggtgcctga ggccgtaaat
      301 gatgcccgga acgctcctgg gaagcgcgac tcccgggtat ggcaccccat atcccagcgt
      361 gtcacaggcc ctgatcttta tcggcacgcc gctttcctcg ctcagtttgc gcagttccag
      421 agcgaaggga acgacaaagc cgtagaagtc ggcacgggtt atgtcttcga aatgacagcg
      481 gggcctgatg ccgacttcga tggcagactt tatgatcccg atataatggt caagagcctg
      541 tttccttgtc atattgagct tcttgaagat gtggtaatcg gagcagctga cgaggatgcc
      601 gctttccttt acccccatat cctttgccag ctggaagtcg cttttggtcg ccctgatcca
      661 gcttgtcacc tcagggtact catatcccag ctcctggcat ttgtacacgg cttccttgtc
      721 cctgtcgctg tacaggaaga actcgcactg gcggattatg cccttggggc cgccaagcct
      781 gtgcagcatt ttataaagtg tcactatctg ttcaacggta taaggttccc ttgactgctg
      841 cccgtccctg aaagtggtgt cggtgatcca tatctcgtcc gccgggaaca tcggcaccct
      901 tctgtggttg aaggcgcatt tcggtatctc gtcatagctg tatatttccc tgtacaggtt
      961 cggagtccgg acatcctgca gctcgtagcg gtacagggac tgctccagcg tattcgtttt
     1021 cttgttgaat atcagcataa caacacccgt ctctttatta aatttgattt tgtataattg
     1081 tatacaatag tacaatcgct gtcaacactt tcttatcggc aattttctgc atcgatgttg
     1141 aaataaggag gattttagca gtttatatcg aaatagatat tatccgtatt atctcagggg
     1201 ggatgaatat ggcagttaag aaaaaggaga aagcaaaatc aattgctgaa caggtatcca
     1261 gccagggaaa gatctactta ctcacatcgg tcgcggtcgg gcttgtaatg agcgccgtat
     1321 acgtactgat aacaggcaga tatgcagaag ctcttctggt actggccaca gccataataa
     1381 cttcccaggt catgcgaatt gcaaatggca tcatgatacg caggatctca cggaaatttg
     1441 aagaagagct taatgtcata aaacagggag attattccag gttcctggat ccgaattctt
     1501 tcggcgtgct gtccggtata ggttcatcgc tcaactccgt gctgagcgac ataaggtcgc
     1561 tgatcgacag cttcttctcg ctgtcccatt cgatcatcca ggcatcgagg aaggtcggat
     1621 cgacagcgga acaggcagtc gccgccatta ccgaaatttc caaaacagtg gacgagatcg
     1681 caaaaggcgc gtcagaccag gcagtcgacg cgtgggaggg cgtccagctt gttgacaagc
     1741 tctccgagca gatcgatttc gtctacaaaa gctacagcga cgtgatggat gaaacgaaca
     1801 acatccacag gctcaacgag gcaggacttg aatcggtcca gatcctccgt gagaggtctg
     1861 agcagagtta caactctacc gaaaagattt tctccgttgt tgaaaacctg acaaacacca
     1921 caaaagatat tggcgctttc gtacagtcca tcgaggatat cgccgagcag accaacatgc
     1981 tcgcgctgaa cgccgctata gaggccgcaa gggcaggtga agccggcaaa ggctttgcag
     2041 tagtcgccga agaagtcagg cagcttgctg accagagcag gcgcttcact gaagagatca
     2101 ttaacctgat ggacaatata aacgtagaat cccagcaggc catacaggcc atggaaatga
     2161 tgaggaaggt ctcgaaagag cagaatgagg ccgttgacag cacaaacaag gcttttggga
     2221 atatagcgga aggcatagtc gtgatcgtgg acaaaataaa ggctgtgaac gatgcggtac
     2281 tggcaatgca gaagggcaag gacaacgtta tatcagcaat cgaaaacatc tcgtcggttt
     2341 ccgaggaaac ggccgcagcg agcgagcagg tcgccgcaac gacagaacag cagcttaagg
     2401 aaatagacga aatgaaggtt gctgccgaac aacttgacga actcgtccag gaactcgaca
     2461 agaagctgaa gaagttcaag atcaagtaaa atgccggttg gtgccggata taaacgatcc
     2521 ccgcttccga tacggagcgg ggatcttcag tttgtcatat ctcgtattcc ttcatgacca
     2581 tcgctgcttc ggcgttcggg aaattggcgg ctgcttcttc agccacgtct ttcagatcat
     2641 agtcaggcca gatatgggtg aggagcagct ttttggcccc tgccatcgcc gcggcttttc
     2701 cgcactgtgc ggccgtcatg tgcggcgcgc cctccgccca gtccttctca aggaaacctg
     2761 cgtcaagcat cagcagatcc gcgccctttg cgaaagtgac cagcccgtcg gtccaggacg
     2821 tgtctcccga gtaaacgaac tttttaccgg cgcattccgc cgataccgca taatccgtat
     2881 acggatgctt catccgcaag aagctgaact taatgtctcc tatggcaagc tgcgtgtcgt
     2941 ctgtaatggc tccgagttcg aaagcgtcct ttacatcgag ccgctcgtat tcctccgatg
     3001 gttctgcggg agcatagacc ctgagcagct tatcgtacag tccccgtttc cttctgagct
     3061 ggatggcgta tttcagcaca tgcatgtcag atacgtggtc gccgtgaaga tgggtcagta
     3121 taacagcgtc gagtttatcg atactgatga ttttctgcat gttggacaat accccgttgc
     3181 cgcagtcgat cagtacgttc gtggtcccgc tctgcagcag gtaacccgag catgcgcttc
     3241 ctgcagccgg atatggtccg ttgtttccca gcacggtcag tttcatgtct cactcctcct
     3301 ttgaaggaac gacccttata atcgatgaga acagttatgt tacattactt gccggccata
     3361 taccggtata taccgattat ataccaaatc atataatagt tgtatcatag tcatgcacag
     3421 atgatacggc atccacagaa aaaacagcaa tcgtattata tccatctatc ggttgcaccg
     3481 gctctaatca ccgtcctgcc ggtctatcca ggtttatctt gctgaatacc agtaccaaaa
     3541 tgtacccaag caccgcgctg aatataccct gcagcgtatt gggcagcagt tccgcgacgg
     3601 ccgcggtcag tccgaaagct tcatcgaaca tgccgagcag aaatgcctcg gcaaggaagt
     3661 atccgacgat catcacagcg gcacccgcca ctgtggatat gatcagcctg ccttgcttca
     3721 aagcgccgct tttgcggatc ttcgaagtaa gcagtccgac cgccagtcct t
//
LOCUS       DAOSCC010000222         3771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15410_length_3771_cov_4.607643, whole genome shotgun sequence.
ACCESSION   DAOSCC010000222 DAOSCC010000000
VERSION     DAOSCC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15410_length_3771_cov_4.607643"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..609)
                     /locus_tag="PLG72_10100"
     CDS             complement(31..609)
                     /locus_tag="PLG72_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81188.1"
                     /translation="MVLMLAFVFIVVISGSDFELTNVVNPGNVGFMTMLVMIGFGSNK
                     GIITLARLLPSNPRTVAFGLQISIYIIALVNNFTFMLLCVLAKQFFLPDIAVLHAAGH
                     LLLNTAFIVILANIMLPVMLLKDRKFLKIVLICLMLPMYFAASETAGYITGKLTGTGM
                     LPATCLISYVVALITCVLCHAAARKAIKAYDA"
     gene            complement(676..1368)
                     /locus_tag="PLG72_10105"
     CDS             complement(676..1368)
                     /locus_tag="PLG72_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81189.1"
                     /translation="MSVLTRSGYLPVSLGLLVVYSLLSYSMGVNTSYHLARYGASTGE
                     IIAMTDDMIRLACSGSLILVPIVLYLLPLINIRDGKIENMPAATLPLSLPVSRREYAY
                     SRFLYAGGSSVVLVILFTGLNAAGVWYRPESFRTEAYVTVRIWFALISVMIHVAGMLL
                     VAASLGAKNFLNLAAAIFLVSVILTWLISSEIIYKIATSPFFWPVAAVSIIAADIISI
                     VSGTFIFERRDI"
     gene            complement(1385..2257)
                     /locus_tag="PLG72_10110"
     CDS             complement(1385..2257)
                     /locus_tag="PLG72_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ81190.1"
                     /translation="MDTVVEMKNVTKDFKNFRLDNVSLTLEKGYVMGLVGANGAGKTT
                     IIKLIMNLLRKDSGSINVFGMDNQKFEKEIKQRIGFVYDENIYPEHLRLEKIGAVISS
                     FYTNWNQERFIKYLKMFELDPKIRLSKLSKGMKMKFAIAVALSHDAELIIMDEPTSGL
                     DPVFRREVLDLLHGIMEDGECSIIFSSHITSDLDKIADVITLVDKGRIVLSMPYDEMM
                     RKYRVLKGPKSLIDRIDPSWLQGVRVNRFGFDALCLEGERAAREFGNTVVLEKPGIED
                     IMVYYTRKAGECSD"
     gene            complement(2568..2936)
                     /locus_tag="PLG72_10115"
     CDS             complement(2568..2936)
                     /locus_tag="PLG72_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ81191.1"
                     /translation="MDIIISNSSLRPIYLQISDQIKKQILSGKLEDEEMLPSIRVLAR
                     ELQISVITVKNAYEELEREGLIESARGKGFYVATKNRELIREKKISLIEDKLAEVIDE
                     CKLLGIGEEELIEMAKLLYG"
     gene            complement(3486..>3771)
                     /locus_tag="PLG72_10120"
     CDS             complement(3486..>3771)
                     /locus_tag="PLG72_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81192.1"
                     /translation="KIAKCHFFISYFTLSGERIGEIPEVERLYREMGGVFKRFDTWSF
                     YILTSHPRLEALVGRKADKKRKLYNGRILCNYYQFFGPPPPKPVMEDDAQ"
BASE COUNT         1022 a          952 c          771 g         1026 t
ORIGIN      
        1 aaaataatta actgaaccga agctaatgct tcaggcatca taagccttta ttgcctttct
       61 tgccgcagca tgacataaaa cgcatgtaat aagtgccaca acataggata tcaagcatgt
      121 cgcaggaagc atccctgttc cggtcagctt tcccgtgata taaccggctg tctcacttgc
      181 agcgaaatac atcggtaaca tcaggcagat caacactatt ttcaggaatt tcctgtcctt
      241 cagcagcatt acgggcagca tgatattggc cagtatgacg atgaaagccg tattgagcag
      301 caaatggcca gcagcatgaa gcacggcaat gtccggcagg aagaactgct tcgcaagaac
      361 acaaagcaac ataaaagtga agttgttgac cagcgctatt atataaatgg aaatctgaag
      421 tccgaaggct accgtcctgg gattcgaagg cagcaatctt gcaagcgtta tgatgccttt
      481 gttggaacca aacccaatca tcacaagcat cgtcatgaac ccgacatttc cgggattgac
      541 gacattcgtc aattcgaaat cactgccgct tataacgaca ataaacacaa atgcaagcat
      601 cagaaccaaa ataaaccctc ttatactgaa aacctcagtg aataatctca ctactgcacg
      661 cattacaacg ccctcctata tgtcccttct ctcgaatatg aacgtaccgg atacaatgga
      721 tatgatatcc gctgcaatta tggatacagc ggcaacaggc cagaaaaacg gcgatgtggc
      781 aatcttatat atgatttccg aagaaatcag ccacgtaagg ataactgaga ccaggaagat
      841 cgccgccgcc aggttcagaa aattctttgc tccaaggctc gccgcgacaa gcagcatgcc
      901 ggcgacatgg atcattacag aaatcagggc gaaccatatc ctgactgtta catatgcttc
      961 tgtcctgaag ctttccggcc ggtaccatac gcctgctgcg ttaaggccgg taaacagtat
     1021 gacgagtaca acagacgaac cccctgcgta caggaacctt gagtatgcat attctctgcg
     1081 gctgacaggc agactgagcg ggagcgtcgc cgcaggcatg ttttcgattt ttccgtccct
     1141 gatattgatg agcggaagga gataaagcac gatagggacc agtatcaggc tgcctgaaca
     1201 tgcgagcctg atcatatcat ctgtcatagc aatgatctct ccggtactgg ctccatatct
     1261 ggccaaatgg taacttgtat ttactcccat cgaatacgac agcagcgaat aaacaacgag
     1321 cagtcctaaa gatactggca ggtatcccga gcgcgtcaac acagacatat tgaacctcat
     1381 cgccttaatc actgcattca cccgctttcc ttgtgtaata taccattatg tcctcgatac
     1441 cgggcttctc aagtacgaca gtattgccaa actccctggc ggctctctcg ccttccaggc
     1501 acaacgcgtc gaaaccgaac ctgttgaccc tgacgccctg cagccatgac ggatcgatcc
     1561 tgtcgatcag cgattttggc cccttcagca ccctgtactt tctcatcatt tcatcataag
     1621 gcatggacag gactatcctg cccttgtcga ccagggttat cacatctgct atcttgtcca
     1681 ggtccgacgt tatatggctt gagaaaatga tgctgcattc accgtcttcc attatgccat
     1741 gcagcaggtc gagcacttcc cgtctgaaaa cgggatcgag gcccgacgtc ggttcatcca
     1801 tgattatcag ttccgcgtca tgcgacagag ccacagctat ggcgaacttc attttcatgc
     1861 ccttggaaag tttcgacagt ctgattttcg gatccagttc aaacattttg agatatttaa
     1921 taaaccgctc ctggttccag ttcgtgtaga agcttgatat cacagcgcct atcttttcaa
     1981 ggcggaggtg ttccgggtat atgttttcat catagacaaa gcctatcctc tgctttattt
     2041 ccttctcaaa cttctggtta tccatcccga atacattgat ggaaccggaa tccttcctga
     2101 gcaggttcat aataagcttg ataatagtgg tcttcccggc accgttggcc ccgaccagtc
     2161 ccattacgta acctttttcc agtgtcagac tgacattgtc cagcctgaag ttcttaaaat
     2221 ccttggtgac atttttcatc tccacaacag tatccataca cgacctccat aaacacttac
     2281 tgaaatgtca tttatatctt atatgcaatc tccgctccct gagccgtcat caccgccagt
     2341 cacctgccac ataccatata ccgactgccg cttgccatat accgtctgcc tcataccatt
     2401 taccgtctac cgcttgctat ataccatctg ccgcttgcca tataccatct gccgcatacc
     2461 atttaccgtc taccgcttgc catatactgt ctgcttcata tagtacaccc cctgtctcag
     2521 gcataccgtc agccaataac ataccaccag ccgtaggcat atcgaaacta gccgtacagc
     2581 agcttcgcca tttcgataag ttcctcttcc cctataccga gaagcttgca ctcatcgatc
     2641 acttcagcga gtttatcttc gatcaggctg atctttttct ctctaatcag ttccctgttt
     2701 ttcgtggcga cgtaaaagcc cttgcccctt gccgactcta ttagtccttc gcgttccagt
     2761 tcctcgtatg cgttctttac tgtaatgacg ctaatctgca gttcccttgc cagaacgcgt
     2821 atcgaaggca gcatctcttc atcctcgagt tttccggata atatctgctt ttttatctgg
     2881 tcggatatct gtagatatat cggcctcaaa ctggaattgg atattatgat gtccagatcc
     2941 acgccgtcac ctcctgtaaa taaaggatcg tatcatacac taactgtata tatagtttat
     3001 atacagtata tactctataa acacttattg tcaagataat tttttctttc agtatcctgg
     3061 acaaataatt cagttatgtg tacatgtcag caaaatcaat taagaattcg gagcaataac
     3121 ataaagaatt tttatattat gtagccaatg catggtcatt tatccatgtt cggtcaatta
     3181 ttgtacacag aactgaaata tttgtccaaa ctgctttcac tttatgaaac atcgggatct
     3241 gcaatgagaa gagaacattt actaattccc gaccgaatag tgcaggctgt ctgtttcagg
     3301 gcaaagctca gttttgtgtg ttaagtgaat ccaggtgatg gataagaaat ggttaagcgg
     3361 gtaaaaaagg tattggggta taaaaggtat tggggtataa aaggtattgg ggtaagaagg
     3421 tattggggta agaaggtatt agggtaagaa ggtattaggg taagaaggta ttaggctaat
     3481 cagacttatt gggcatcatc ttccatgacc ggtttcggcg gcggaggtcc gaaaaactgg
     3541 tagtaattgc acagtatcct gccgttgtaa agctttctct ttttgtccgc cttgcgtcct
     3601 accagtgctt ccagccgcgg gtgcgatgtc aggatataga acgaccaggt atcgaacctt
     3661 ttgaaaacac cgcccatctc cctgtacagc cgttccacct cgggtatctc tccgatgcgt
     3721 tcgccggata gtgtaaagta acttatgaaa aaatgacatt tagcaatttt g
//
LOCUS       DAOSCC010000223         3730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15658_length_3730_cov_4.658503, whole genome shotgun sequence.
ACCESSION   DAOSCC010000223 DAOSCC010000000
VERSION     DAOSCC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15658_length_3730_cov_4.658503"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="PLG72_10125"
     CDS             complement(<1..1951)
                     /locus_tag="PLG72_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ81193.1"
                     /translation="MNYHVSKTGSDLNPGTESQPFLTISKAAYVAKPGDTITVHEGVY
                     REWVSPKRGGTKAQPIVYQAAEGEKVVIKGSEVITDWEKDGNIWKTVIDNKFFGDFNP
                     YSEVLFGDWLFTKDRVFHLGEVYLDGHAMYEAVSVEEVRNPQKSKTSKEPEFSVYKWY
                     AEVDDRCTTIYANFHGEDPRNGNVEINVRRFCFWPENPGRNYITVRGFIMQHAATQWA
                     PPTALQEGLIGPHWSKGWVIENNIISDSRCCGISLGKEESTGQNEWTTIFTKVGHQRE
                     QEVIFRALNNGWCKDNIGSHTVRNNIIYNCGQAGIVGHLGCVFSLIENNHIYNIRRKR
                     EFEGAEVGGIKLHAAIDTIIRNNIIHDSFRGVWLDWQAQGTRVSGNIMFGNDSEDMLV
                     EVSHGPTMIDHNIMLSGHSFKTLSQSCALVHNLIAGTISAAQDLNRHTPYHVPHSTSV
                     AGMNRFTGGDDRYYNNIFLREKGDTRPDEPYLEGFFGNDILKPEDIKPNTCAFMSYPV
                     GTAVYNDYPGPDDDPPWEIFRRLFTGELREGEEPGQLFAILEKNKQLPVYIENNAYFN
                     RALPYKKEKGAKVYEDSGIEFTIDRESKKVIIEITKPEMLIESAGEIITTAKLGYGFH
                     TEQLFEKPDGTPYCFDTDFFGEPRGK"
     gene            complement(2052..3308)
                     /locus_tag="PLG72_10130"
     CDS             complement(2052..3308)
                     /locus_tag="PLG72_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ81194.1"
                     /translation="MDEEFVLSEEAGANTAKDERLWTAPYISLISLYTLVSMGFYMVM
                     PLLAKYAIQHGASVPAAGVIVGMFSITALFVRPAAGIISDRFNKKTVFIAATATIGLS
                     LTGYGLSPGIAPLVVFRLIHAVAFSVNSTVSLALAAQIVPKKRTGEGIGYFGIGHIIA
                     TAVGPAVGQYIGESFGLSATFLIAGAATLAASAMIFALPVNGQKAPDPGEKQEKGIVG
                     ETDKRIRIADLIAVQVLPLAFFGSVFSMFNGTVGSYLVLLGEERGIADISLYFTINAA
                     ALILVRFTAGRVYDRYGLSAVLIPSFILAAAAAAFIGFAQALPIILAAAVIKAFAQGS
                     AQPSIQAECIRMLPNGRSGVATSTYYIGADIGQGLGPMLAGAIASEWGYEVMFASCSG
                     VFLAALLFYCAGLYKQGRAGRGQPAD"
     gene            complement(3391..3702)
                     /locus_tag="PLG72_10135"
     CDS             complement(3391..3702)
                     /locus_tag="PLG72_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ81195.1"
                     /translation="MAQRLQCLWRGSTLLPYDLLALGALAVFCGHGIRIPEDMEVIVY
                     GNNESLEIHSPSITTVQYPTREISGECINVMAQLLDKPGTGPVRRIVETPFIFRESCP
                     E"
BASE COUNT          769 a         1062 c          952 g          947 t
ORIGIN      
        1 tctttccgcg cggttcgcca aagaagtccg tatcgaagca atacggcgtg ccgtcaggct
       61 tctcaaacaa ctgctcggta tggaagccgt acccgagttt cgctgtcgtg attatttcac
      121 cggcactttc gataagcatt tcaggcttcg ttatttcgat aattaccttt ttgctctccc
      181 tgtctatcgt gaactcgatc ccggaatcct cgtacacttt tgctcccttt tcttttttat
      241 agggcagcgc cctgttgaag taagcgttgt tttcgatgta aacggggagc tgcttgttct
      301 tttcaaggat cgcaaacagc tggccgggtt cttcgccctc cctcagttca cctgtgaaca
      361 gcctcctgaa tatctcccac ggcggatcgt catccggccc gggatagtca ttgtatactg
      421 ccgttcccac agggtatgac ataaacgcgc aggtgttcgg ttttatgtct tcgggcttta
      481 aaatgtcatt gccgaagaag ccttccaggt aaggctcgtc cggacgggta tcgccctttt
      541 ccctgaggaa gatgttgttg tagtaacggt cgtcaccgcc ggtaaacctg ttcattccgg
      601 caaccgaagt gctgtgcggg acatggtagg gcgtgtgcct gttgagatcc tgggcggcgg
      661 atatcgtccc ggcaataagg ttgtggacga gtgcgcagct ttggctgagc gttttgaacg
      721 agtggcccga aagcatgatg ttgtggtcga tcatcgtggg cccgtggctc acttcgacaa
      781 gcatgtcctc gctgtcgttg ccgaacatta tattgccgct cacgcgcgtc ccctgcgcct
      841 gccagtcaag ccagacgccc ctgaaggaat cgtggatgat gttgttcctg atgatcgtgt
      901 cgatggcggc atgcagcttg atcccgccta cctcggcgcc ttcgaattcc ctcttgcgcc
      961 ttatattgta gatatgattg ttttcaatga ggctgaaaac acatccgaga tgcccgacga
     1021 taccggcctg tccgcagttg taaatgatat tgttgcggac ggtgtggctt cctatgttgt
     1081 ctttgcacca gccattgttc agcgccctga atatcacttc ctgctccctc tggtggccca
     1141 ccttggtgaa aatggtcgtc cactcgttct gcccggtgct ttcctctttt ccaaggctga
     1201 tgccgcagca gcgggaatcg cttatgatgt tgttctcgat cacccagccc ttgctccagt
     1261 gcgggccgat caggccttcc tgcaacgcgg tcggcggagc ccactgggta gctgcgtgct
     1321 gcatgatgaa gccccggaca gttatgtagt tgcggccggg attttcaggc cagaagcaaa
     1381 aacggcgcac atttatctca acattcccgt ttctcgggtc ctccccatgg aagttcgcgt
     1441 aaatggtggt acagcggtca tccacttcgg cataccattt gtaaactgaa aattccggtt
     1501 cctttgatgt cttcgatttc tggggattgc gcacctcttc gacggaaacg gcctcgtaca
     1561 tggcgtgccc gtccaggtag acctccccaa ggtggaatac cctgtctttt gtaaacagcc
     1621 agtcgccgaa caagacctca ctgtacgggt tgaaatcccc gaagaattta ttgtctatga
     1681 cggtcttcca tatgttgccg tccttttccc aatccgttat gacctcggag ccctttatta
     1741 cgactttttc gccctcggcc gcctggtaga ctatcggctg cgccttggtc ccgccccgct
     1801 tcgggcttac ccattcgcgg tagactcctt catggactgt gatggtatct cccggtttgg
     1861 caacatatgc tgctttgctg atggttaaga aaggctggga ctctgttccg ggattcagat
     1921 cgctccctgt cttgctgaca tggtaattca tagcatcctc cttgtggatc aggcttgaaa
     1981 tggtttattc attaacacat atgcttttcc tgcaaatata ttatagcaca attttccggg
     2041 accatgtccg gtcaatccgc cggctgcccg cgcccggcgc ggccttgttt atacagtccg
     2101 gcacagtaga acaggagggc cgcaaggaaa actccggaac atgaggcaaa catgacttca
     2161 tagccccatt ccgatgcgat tgcccctgcc agcatcggtc caagtccctg tccaatgtct
     2221 gcccctatgt agtatgtgct cgtggcgacc ccgctcctgc cattcgggag catccggatg
     2281 cattcagcct ggatcgacgg ctgtgccgac ccctgggcaa aggcttttat cacggctgct
     2341 gccagtatta tgggaagtgc ctgcgcgaac ccgatgaaag cagcggcagc ggcagccagt
     2401 atgaatgacg ggatcagcac ggcggataag ccatacctgt catagaccct gccggctgtg
     2461 aatctcacga ggatcagcgc tgccgcgttg atggtgaaat agaggctgat gtccgcgata
     2521 cctctttcct cgccaagaag taccaggtat gaccctacag tgccgttgaa catcgaaaaa
     2581 acgcttccga aaaacgcaag cggcaatacc tgtacggcaa tgagatcggc aatgcggatc
     2641 cttttatcgg tttccccgac tatacccttt tcctgttttt ctccgggatc gggcgctttt
     2701 tgcccgttga ccggcagggc aaaaatcatc gcagaggccg caagcgttgc cgcacctgcg
     2761 atcagaaacg ttgcggagag tccgaaactt tctcctatat attgcccgac cgccggtccc
     2821 acggccgtgg caattatatg tccaatgcca aagtatccga tcccttctcc tgttctcttt
     2881 ttcgggacta tctgtgcagc gagcgccagg cttactgtgc tgttcacgga aaaagcgacc
     2941 gcatggatga gacggaaaac caccagcggt gcaatgcctg gcgaaaggcc gtaacctgtg
     3001 agcgataacc cgatggtcgc tgtcgccgcg ataaatacgg tttttttgtt gaacctgtct
     3061 gaaatgatcc ctgcagccgg cctcacaaag agcgcagtta ttgaaaacat gccaactatg
     3121 acgcccgccg ctgggaccga cgccccgtgc tgtattgcat atttcgcgag cagcggcatg
     3181 accatgtaga aacccatgga tacaagggta tacagtgaaa tcagcgaaat atacggcgcc
     3241 gtccacagcc tttcatcttt agcggtgttt gctccggcct cttctgataa aacaaattct
     3301 tcatccatac ctgtctcgtt ccccctgaca atcaccagtt ctcggaaaaa taccggcctg
     3361 caccggtcca ggcggcacag gccgcccata tcactccggg cagctttcac ggaaaatgaa
     3421 cggtgtttcc acgatcctcc gcacgggtcc ggtcccgggc ttatccagca attgcgccat
     3481 gacgtttata cattccccgg atatctcccg cgtcgggtat tgcacggtcg tgatcgacgg
     3541 gctgtggatc tccagcgatt cattgttgcc atatacgatg acctccatgt cctccggaat
     3601 gcggatcccg tgcccgcaaa aaacggcaag agcgcccagc gccagcagat cgtaaggtag
     3661 aagagttgaa ccgcgccaga ggcattgtaa cctctgtgcc acatgatgcc cgttccccaa
     3721 gtattatatg
//
LOCUS       DAOSCC010000224         3711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15784_length_3711_cov_4.190919, whole genome shotgun sequence.
ACCESSION   DAOSCC010000224 DAOSCC010000000
VERSION     DAOSCC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15784_length_3711_cov_4.190919"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..2070)
                     /locus_tag="PLG72_10140"
     CDS             complement(124..2070)
                     /locus_tag="PLG72_10140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ81196.1"
                     /translation="MPHDYLLSICMMVRDEEKNLKRCLDAVKSLVDRDDVELIIVDTG
                     SKDSTVDIAREYTDKVYFHPWNNNFAAMRNVTVSYARGEYILIMDADEVLTDPASLYK
                     YISDESLKDYNTFTVKVKNFDSSGGYTVITQERVFKNDGSFGYEGSVHNQPKFKKPVL
                     HTDIYMDHYGYLFHDRELREKKFVRTAGILKKELEKNPNSIYYRFQLARSYSAHRDKK
                     EAFEEIRKAYRLLTANKEVQRLYAYVYGTYCIICFENNEFDEVIRACGEGLELRSEYI
                     DLYYLMAAAYARTGRDEEAFAAYEKYIELVKQYDDLAISADRSMEMYFLSEKYQDGAY
                     AFMANYMYQKGKYNECYGYALEINDQRTKSERIVRVLFKLKKYDELKSFYDSIIGNKP
                     LREATELLIEAEASALNNDERKKIYELFSNGDGHYSILNRIRISDGDEKRQLVSDALR
                     NLDFNELPDYYADMFADIDKDPRPVISLFKKLRKVKIKQFVKRLVDRDRKLENFFEQY
                     LLDETVRPDDHSSLKVYIGIAYTLLVIKAVDVRESGTEPLDRYLEIFEKYSKNGFSHI
                     MTLYRPERLRIYYSTMEDREDAFFIALHYAEEACGKGDYKAGVKYFRDAARANAYMAC
                     YMKKYKDRLFRDVDGVDGEEEYND"
     gene            complement(2236..3009)
                     /locus_tag="PLG72_10145"
     CDS             complement(2236..3009)
                     /locus_tag="PLG72_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOJ81197.1"
                     /translation="MIINNNISAINTRNQLYITNTAAGKSMEKLSSGKRINRAADDAA
                     GLSISEKMRAQIRGLNQAARNAQDGISLIQTAEGALDEVSNMLVRLKELAVQKANGTY
                     NNDDKSNLNLEMDSLGDEIDNIFTSTKFNGFTVLTTSAKIVISDDGSTSLTIEKTTTT
                     GIVGLSSASSVGKIETAIKNVNKARATYGALQNRLESTVRNLKTTAENLQAAESRIRD
                     TDMAEEMSNFTKYSILIQAGTAMLAQANMAPQSVLSLLR"
     gene            complement(3182..>3711)
                     /locus_tag="PLG72_10150"
     CDS             complement(3182..>3711)
                     /locus_tag="PLG72_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOJ81198.1"
                     /translation="VSNMLVRLKELAVQKANGTYNTDDVSNLKLEMASLGKEIDNIFD
                     NTKFNGFKVLTTSAKIVISDDGSTALTIGTTDTNGIKGLGSNSTVGGIETAIKNVNKA
                     RATYGAKQNRLESTVRNLKTTAENLQAAESRIRDTDMAEEMSNFTKYSILIQAGTAML
                     AQANMAPQSVLSLLR"
BASE COUNT          757 a          971 c          812 g         1171 t
ORIGIN      
        1 tggtccccgg gcatattctc ttcaagtttg ccgatggccg cttttgcttc ttccaaatgc
       61 ccgtcctgta tcagcttctc gatctttgcc tttacagacc tgatcttttc ctgcataccc
      121 tggtcaatca ttgtattctt cctcaccgtc cactccatca acatccctga acagcctgtc
      181 cttgtatttc ttcatataac atgccatata agcatttgcc ctcgcagcgt ccctgaagta
      241 tttcacacct gctttatagt cgccctttcc gcatgcttcc tcggcatagt gcagggctat
      301 gaaaaaagca tcctccctgt cttccatcgt gctgtaatat atgcgcagcc tttcaggacg
      361 ataaagagtc atgatatgac tgaaaccgtt ttttgaatat ttctcaaata tctcaaggta
      421 ccgatccagg ggctcggtgc ctgattccct gacatcgacc gctttaatta ccagcagggt
      481 atacgctata ccgatataca ccttcagact gctgtggtca tccggcctga cagtttcatc
      541 caaaaggtat tgctcgaaaa agttttcaag cttcctgtcc ctgtcgacca atcgtttgac
      601 gaactgcttt atctttactt tcctgagttt cttgaataaa gagatcaccg ggcgcggatc
      661 cttgtcaata tcggcaaaca tatccgcata gtaatcaggc agctcgttga aatccaggtt
      721 tctcaaagca tctgaaacca gttggcgttt ttcatcccca tcgcttatgc gtatcctgtt
      781 caggatggaa taatgcccgt ctccgttgct gaaaagctca tatatcttct ttctttcatc
      841 attgttcagc gcggatgctt cggcttcgat cagaagctcc gtggcttcac gaaggggttt
      901 attgccgatg atggagtcat aaaacgattt gagctcatca tactttttca gtttgaacag
      961 caccctgact attctctcgg atttcgtcct ctggtcattt atctccaaag catacccata
     1021 gcattcattg tatttccctt tttggtacat gtagtttgcc atgaaggcat atgccccgtc
     1081 ctggtatttt tcactcagga agtacatttc cattgacctg tcggccgata tggcaaggtc
     1141 gtcatattgc ttcacaagtt cgatatattt ttcatatgcg gcgaacgctt cctcatctct
     1201 gcctgtcctt gcgtatgcag cagccataag gtaatacagg tcaatgtatt cggaccttaa
     1261 ctccaggcct tcaccgcagg cccggatcac ttcgtcgaac tcgttattct cgaagcatat
     1321 tatgcagtat gttccatata cgtaggcata caatctttgc acttccttat tggccgtcaa
     1381 cagccggtat gccttgcgga tctcctcaaa agcttccttt ttgtcacgat gcgcactgta
     1441 acttcgtgca agctggaacc gataatatat gctgttcgga tttttctcca gttctttctt
     1501 gagtatcccg gccgtcctga cgaacttctt ttccctgagt tccctgtcgt ggaacaaata
     1561 gccgtaatgg tccatgtata tatccgtgtg cagcacgggt tttttgaatt tcggctggtt
     1621 atgcacggaa ccttcgtatc cgaatgaccc gtcattttta aagaccctct cctgggtgat
     1681 gaccgtatat cctcctgaag aatcgaagtt tttgaccttt acggtgaagg tattgtaatc
     1741 tttcagactt tcgtcgctga tatacttata aagcgacgcc ggatccgtca gtacttcgtc
     1801 ggcatccatt atcaggatat attcgcccct ggcgtatgag acggtcacgt ttctcattgc
     1861 tgcgaagtta ttgttccagg gatggaaata tactttatct gtatactcac gtgcaatgtc
     1921 aactgtactg tcctttgatc ccgtatcaac gatgatcagt tccacgtcat ccctgtcaac
     1981 aagggatttc actgcatcca ggcagcgttt caggtttttc tcctcgtccc tgaccatcat
     2041 gcatatgctc aaaaggtaat catgcggcat atttatttca tctccaggtt tctgtcatac
     2101 tttttcatcc gtatgaagtt acgttatatt tatcggctat aatgcgctga tgcattagaa
     2161 catttgtgca ccgtacagct atttttgcgc agaaaaaaag gtcccggtgt ccgggacctt
     2221 ccgtaatggc ttcgcttatc tcagcaatga cagtacgctc tgcggtgcca tattggcctg
     2281 tgccagcatg gctgtgcccg cctggatcag gatgctgtac ttcgtgaagt tcgacatctc
     2341 ctcggccatg tcggtgtccc tgattcgcga ttccgcagcc tgcaggttct ctgcagtggt
     2401 cttcaggttc ctgacggtgg attcgagcct gttctgcaaa gctccgtagg tagctcttgc
     2461 cttgttcaca ttctttatgg cagtttctat ctttccgacc gacgaagcgc tgctgaggcc
     2521 aactattccc gtggtggtcg tcttctcgat ggtgagcgat gttgatccgt cgtcggaaat
     2581 gacgatcttg gccgaagtcg tcagtactgt gaagccattg aacttagtgg acgtgaagat
     2641 gttgtcgatc tcgtcgccga gtgaatccat ctcaaggttc aggttagact tgtcatcgtt
     2701 gttgtacgtc ccgtttgcct tctgtaccgc cagttccttc agcctgacca gcatgttgct
     2761 gacttcgtcg agcgcacctt cagcagtctg tatcagcgaa ataccgtcct gggcgttcct
     2821 tgccgcctgg ttcagacccc tgatctgcgc cctcatcttc tcggaaatgg acaggcctgc
     2881 agcgtcatct gccgcacggt tgatcctttt gcccgacgac agcttttcca tcgatttgcc
     2941 cgctgccgta ttggtgatgt acagctggtt cctggtgttg attgcagaga tgttgttgtt
     3001 gataatcata tgatcatcct ccctgatttt ttatacggga atccattccc gctttatcag
     3061 ataaaccgcc ctgcatatgc cagcgcgcgc tccggtatat gaaaggcgat atatcctgcc
     3121 ggtaatgtgg ataaagagac ccattattca tgagtctctt tacccgatat tgtcacgatc
     3181 gttatctgag caatgacagt acgctctgcg gtgccatatt ggcctgtgcc agcatggctg
     3241 tgcccgcctg gatcaggatg ctgtacttcg tgaagttcga catctcctcg gccatgtcgg
     3301 tgtccctgat tcgcgattcc gcagcctgca ggttctctgc agtggtcttc aggttcctga
     3361 cggtggattc gagcctgttc tgctttgctc cgtaagtagc ccttgccttg ttcacattct
     3421 ttattgcagt ttctatgcct cctactgtgg aattactgcc aaggcccttt ataccgttgg
     3481 tgtcagttgt cccgatggtg agcgccgttg atccgtcgtc ggaaattacg atctttgctg
     3541 aagtcgtcag cactttgaag ccgttgaact tggtattgtc gaagatgttg tcgatctctt
     3601 ttccgagtga tgccatctca agtttcaggt tgctgacgtc atccgtatta tatgtgccgt
     3661 ttgccttctg taccgccagt tccttcagcc tgaccagcat gttgctgact t
//
LOCUS       DAOSCC010000225         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15788_length_3710_cov_4.189330, whole genome shotgun sequence.
ACCESSION   DAOSCC010000225 DAOSCC010000000
VERSION     DAOSCC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15788_length_3710_cov_4.189330"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLG72_10155"
     CDS             complement(<1..472)
                     /locus_tag="PLG72_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOJ81199.1"
                     /translation="MSVLEAIVYGILQGLGEFLPISSTAHITLAPWFFGWKDPGLAFD
                     IALHLGTLAAVVIFFWKDWIELIKAGLTDTKLPEGRLFWYIVLACVPGGILGLIFEEH
                     VETTFRNPLLIGIMLIVMGIVIYAADKYSRSEIELLDIGPRRSFLIGVSQALAMI"
     gene            complement(683..2380)
                     /gene="lonB"
                     /locus_tag="PLG72_10160"
     CDS             complement(683..2380)
                     /gene="lonB"
                     /locus_tag="PLG72_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOJ81200.1"
                     /translation="MLTNTFLIIQFFFSVIIGIYFLNLLKSQQCNKSAIEKESRKELE
                     KLRRLKEIKLSEPLAEKTRPASISDIIGQEQGLKALRAALCGPNPQHVIIYGPPGVGK
                     TAAARVILEEAKQMVISPFRRDAKFVEVDATILRFDERGIADPLIGSVHDPIYQGAGA
                     YGVAGIPQPKPGAVTKAHGGILFIDEIGELHPIQMNKLLKVLEDRKVMLESAYYSSED
                     TNIPAHIHEIFQKGLPADFRLVGATTRTAEELPPALRSRCVEIYFRPLTADEIATIAC
                     NAAKRGGFKMEEGCEQLIAKYAQNGRDAVNIIQIAGGVIQVEGRSMITRKDIEWVLEF
                     GHYSPRIDKKVSAQEKQVGCINGLAVFGNATGVVIDIEVTAMKAQSGKGSIKVTGIVE
                     EEEMDGKGHKLKRISSAKSSVENVLTVLKKFLNVDAREYDIHLNFPGGIPIDGPSAGI
                     AIAAAIYSAVLNLPVSNDIALTGEISIRGKVKPVGGVAAKVEAARQAGINKVLIPKEN
                     WQETFGNIGIEVVPVDDITEVINHIFYSDRLPQEGGIPVQKPAISVFAASPAIEKSF"
     gene            complement(2748..>3710)
                     /gene="clpX"
                     /locus_tag="PLG72_10165"
     CDS             complement(2748..>3710)
                     /gene="clpX"
                     /locus_tag="PLG72_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ81201.1"
                     /translation="ILMVGPTGCGKTYLAQTLARLLNVPFAIADATSLTEAGYVGEDV
                     ENILLKLIQAADYDIERAEKGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTVASVPPQGGRKHPHQEFIQIDTTNILFICGGAFDGLEKIIKNRIGTRAIGFGAKIE
                     SKKEEDIGKILAQVLPQDLLKYGLIPEFVGRLPIIVTLDPLDRDALVKILTEPKNALV
                     KQYQKLFEMDDTLLEFEPDALEAIADKALARNTGARGLRAIIEEIMLDVMYDIPSSNN
                     VEKCIVTKDTVENKTKPALIINENRKPLKKATGKKSRVKKESAS"
BASE COUNT          766 a         1034 c          865 g         1045 t
ORIGIN      
        1 gtatcatggc aagtgcctgc gagacgccta tcagaaagct ccttctgggt ccgatatcca
       61 acagttcgat ctcggaccgg gagtatttat cggcagcata gatcacgatg cccatcacta
      121 taagcattat cccgatcagc agcggatttc tgaacgtggt ttccacgtgt tcttcaaaaa
      181 tcagcccaag aatcccgccc ggcacgcatg ccaggactat gtaccagaac agcctgccct
      241 caggcaattt cgtgtcggtc aggccggcct ttatgagttc gatccagtct ttccagaaaa
      301 atatgaccac cgcagccagt gtgccaagat gcagtgctat gtcaaacgcc agccccggat
      361 ccttccatcc gaaaaaccac ggcgccagcg taatgtgcgc tgtgctcgat atcggcagaa
      421 actcgcccaa tccctgcagg atgccataaa ctatcgcttc aagtacactc acaatgatcc
      481 cccttcggat accagtttat attataattt gcagaactgt cccctttttg gagaaacgtc
      541 ccttttagaa taccacataa ttgccggcga gtatatacgg ccagtatata aaagctgcaa
      601 ctatgtgcgt acagtcccgg gaacggctca aatcccgcta ctgtagatat atacttcgta
      661 tcgggtttta tgtctgcacc tgtcaaaagc ttttttcgat ggcaggggaa gcggcaaata
      721 cggatatcgc cggtttctgt accggtattc ctccttcctg tggcaatctg tcggagtaga
      781 aaatatggtt tatcacttcg gtgatgtcat ctacaggcac cacttcgatc ccgatgttcc
      841 cgaacgtctc ctgccagttt tccttcggga tcaggacctt gttgatgcct gcctgccgcg
      901 cggcctccac tttggctgcc actccgccca cgggttttac cttgcctctg atggatatct
      961 cgccggtcag ggcaatgtcg ttgcttacag gcagatttaa gaccgccgaa tatatggctg
     1021 cggctatggc tatgccggca gacgggccgt caatgggtat cccgcctgga aaattgagat
     1081 ggatgtcgta ttcccttgca tctacattaa ggaacttttt aagcacagta agcacatttt
     1141 cgacagaaga ctttgcggaa cttatcctct tcagcttgtg gcctttgcca tccatctcct
     1201 cctcttccac tataccggtg acctttatgg accccttgcc ggactgggct ttcattgcgg
     1261 ttacttcaat atcgatgacg acaccggtgg cattgccgaa aacggcaagc ccgtttatac
     1321 agccgacctg tttctcctgt gccgagacct tcttatcgat ccttgggctg taatgcccga
     1381 actcaagcac ccactctata tccttgcggg ttatcatgct ccgaccctcc acttgtatca
     1441 ctccgccggc tatctggatt atgttgacgg cgtccctgcc gttctgcgca tatttcgcga
     1501 tcagttgctc acagccttcc tccattttga atccgcctct ttttgcggcg ttacaggcta
     1561 tcgtggcaat ttcgtcagct gtgagcggcc tgaagtatat ctcgacacac ctggagcgaa
     1621 gagcaggcgg cagttcctcg gcagtcctcg tcgtggcgcc gacaagcctg aaatcggccg
     1681 gcagcccttt ctggaatatt tcatgtatgt gtgcagggat attggtatcc tctgaactgt
     1741 aatatgcgct ttcaagcatg accttccggt cctccagcac tttcagcagc ttgttcatct
     1801 gtatcggatg cagttccccg atctcatcga tgaacagtat gcctccatgc gccttggtca
     1861 cggctccggg tttgggctgg ggaatgcctg ccacgccgta tgcgccggct ccctggtaaa
     1921 ttggatcatg taccgaaccg atcagggggt cggcgatgcc gcgctcatca aaccgcagga
     1981 tggtggcgtc cacctcgacg aacttggcgt ctcttctgaa aggagatatg accatctgct
     2041 ttgcttcttc aagtatgacc cttgccgcag cggtcttccc gactcctgga gggccgtata
     2101 tgatgacatg ctgaggattg ggcccgcaga gagctgcccg taaagccttg aggccctgtt
     2161 cctgtccgat tatatccgat atcgacgcag gcctcgtttt ttcagccaaa ggctcggaca
     2221 gctttatttc tttcaacctg cgcagcttct ccaattcctt ccgggactcc ttctcaatgg
     2281 cgcttttgtt gcactgctgc gatttgagca gattcaggaa gtatatgcct attattaccg
     2341 aaaagaaaaa ctgtattatc agaaaggtat tggtcagcat tccatctcct cctaggatct
     2401 tgccgcgtct taaatgtttt tactagaata gtattgacat tacagataaa tttatccgta
     2461 ttggcttcgg agacggtccg cgtgctgccg gatatggctt cagcgctcct tatgagcatt
     2521 taatttgcag aaggatcctg aaacttgacc agaggatctt gactttctga accggattct
     2581 tcccggtccc tggaccgcga atgctttcca ggaacatatt ctgtccggag aacatatatg
     2641 ctgtctgggc atagaaaacg ggcatgacct tttggattca tgcccgcttt tatcagcgtt
     2701 caaattccca tatatccagg gatgaccccg tttcaaagac aaacggctca ggacgcgctc
     2761 tccttcttga ctctcgattt tttgcccgtc gccttcttga gcggcttcct gttctcgttt
     2821 attatcagtg ccggtttcgt cttgttttca acagtatctt tcgtaacgat gcacttctcg
     2881 acattgttcg aagaaggtat gtcatacatg acatcaagca ttatctcttc tatgatcgcc
     2941 cgaagtcctc tcgcccctgt gttccttgcc agcgctttgt ctgctatcgc ttcgagggcg
     3001 tccggttcga actccagcag cgtgtcgtcc atctcaaaga gcttctgata ctgtttgaca
     3061 agcgcgtttt tcggttcggt cagtatcttc accagggcgt ccctgtcgag gggatccagt
     3121 gtgactatta tcggcagcct tccgacaaac tcaggtataa gcccgtattt gagcagatcc
     3181 tggggcaata cctgggccag tatcttgccg atatcctctt cctttttgct ttctatcttt
     3241 gcgccaaatc ctatggccct ggtccctatc ctgttcttta ttatcttttc caggccgtcg
     3301 aatgctcctc cgcagatgaa caggatattc gtcgtgtcga tctggatgaa ctcctggtgc
     3361 ggatgtttcc ttccgccctg cggcggtacc gacgccaccg taccttcaag tattttcagg
     3421 agcgcctgct gtacgccttc gccgctgaca tctcttgtga tcgaagggtt ttcggacttc
     3481 cttgctatct tgtcgatctc gtcgatatat atgatgccct tttctgcacg ctcgatatcg
     3541 taatcggctg cctggataag tttcagaagt atgttctcta cgtcctctcc cacgtagcct
     3601 gcctctgtca gtgaagtggc gtcagctatg gcaaaaggca cattgagaag cctggccagc
     3661 gtctgtgcca ggtaagtctt gccgcagcct gtcggtccga ccatcaggat
//
LOCUS       DAOSCC010000226         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15826_length_3702_cov_5.917741, whole genome shotgun sequence.
ACCESSION   DAOSCC010000226 DAOSCC010000000
VERSION     DAOSCC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15826_length_3702_cov_5.917741"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLG72_10170"
     CDS             complement(<1..575)
                     /locus_tag="PLG72_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ81202.1"
                     /translation="MAVRYSGPDERVFLLDIGIDLGTASVLVYVKGRGIVLKEPSVVA
                     LDKTSNKLLAVGEEARRMLGRTPGNIVAIRPLRDGVISDYDITERMLKYFINKICGGR
                     LLRLFRPRIMVCVPSGVTEVERRAVIDATMQAGARKTYLIEEPIAAAIGAGIDIAKAC
                     GSMIVDIGGGTTDIAVISLGGIVVSTSIKVAG"
     gene            complement(865..1128)
                     /gene="spoIIID"
                     /locus_tag="PLG72_10175"
     CDS             complement(865..1128)
                     /gene="spoIIID"
                     /locus_tag="PLG72_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOJ81203.1"
                     /translation="MKGYIEERAIELANYIIDKKTTVRAAAKKFGISKSTVHKDVTER
                     LMKISPELAEKVRIILEENKAERHIRGGEATKAKYQEAKTKAG"
     gene            complement(1538..2374)
                     /locus_tag="PLG72_10180"
     CDS             complement(1538..2374)
                     /locus_tag="PLG72_10180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ81204.1"
                     /translation="MKKSFPDEKGNKFREFLDKKGFYIVLLLCIAVVAGTAVYVTSRR
                     SLDKTPEYESENIAEDDMGDIPAEDAALNTDEKLTDLDPDEELVSRPGENESDIGSPA
                     SVTKDGELSGEPDESSKKDTEPKDEAPGKGDSKKQSAPVKQSFIMPVNGEISFEYAMD
                     RLVYSKTLEQWRVHPGIDIAADRGTPVKAVADGVISEVRNDKFYGISVVIDHGDGLKT
                     LYRNLASDEAVAVNQKVNQGEIIGSIGNTAMDESSEQPHLHFEVLVDDENVDPNDYLP
                     IK"
     gene            complement(2478..3503)
                     /gene="spoIID"
                     /locus_tag="PLG72_10185"
     CDS             complement(2478..3503)
                     /gene="spoIID"
                     /locus_tag="PLG72_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOJ81205.1"
                     /translation="MKKFFYYTSVMVLIVILLPAIIVRSCSPEPAPPQEPPAAEIPEK
                     VFEITVYNKDTGSSETMDLEEYIKGVVAAEMPADFDLEALKAQAVAARTFAYGRMTGA
                     YRSKQGVHDGIEICTDSTHCQAWVSKENARKKWGMLFAARNWNKIERAVDETKGLIMV
                     YQGNVINALFHASSPGRTENAEDVWNGKSVPYLRSVVSNDEASKGYITTVKISGDELA
                     EKLLEAWPDAELPEDIANGIKIVDLTEGGRVKTLKIGNITMKGTEFRSLLGLRSACFS
                     IASGDDGMISITTTGHGHGVGMSQWGANALAKTGGTFREILTHYYTGIDIVSISDLEA
                     AARDSGN"
BASE COUNT          813 a         1010 c          816 g         1063 t
ORIGIN      
        1 cccgctactt tgatggaggt cgacacgacg atgccgccca gcgatatgac ggctatatcg
       61 gtggttcctc cgcctatgtc cactatcatg ctgccgcaag ctttggcgat atcgatgccg
      121 gctcctatcg cggcggcgat gggttcctcg atcaaatatg tcttcctggc gccggcctgc
      181 atcgtagcat cgataacggc acgcctttcc acctctgtca ccccgctggg gacacagacc
      241 attatcctcg gtctgaacag tctcagcagc cttccgccgc atatcttgtt gatgaaatat
      301 ttcaacatcc tctctgtgat gtcgtaatct gagataacac catcccgcaa cggtcttatt
      361 gcgacaatat tccccggagt cctgccgagc atccttcgcg cttcttcacc aactgcaaga
      421 agcttgttgg aggttttgtc caaagcaacc acagagggct ccttcagtac tatccctctt
      481 cctttgacat aaaccagcac ggatgcggta ccgagatcta tgcctatatc caacagaaac
      541 accctttcgt ccggcccgct gtatcttacg gccattatag ttagttatat aaggatagaa
      601 aattttgcaa ctgttgataa aataatctta tcatataatc acaattatgc aagagaaaaa
      661 ggttcggagc atggaaaggg gacactcctc acctaggaag tattcctttc cggtcgagga
      721 gtgtcccagg agcaaggtaa ggggacactc ctcacctagg aagtattcct ttccggtcga
      781 ggagtgtccc catgtgttca aatgcgtgac attcctcaac ccgagaggaa tacctctttg
      841 ctgaggagtg tcaccaaacc ttgttcatcc ggctttcgtt ttggcttcct ggtacttggc
      901 tttggttgcc tctcctcccc tgatatgcct ttccgccttg ttttcctcga gtatgatcct
      961 cactttttcg gcaagttcgg ggctgatctt cattagtctt tccgttacat ccttatggac
     1021 tgtgcttttg ctgataccga attttttcgc tgcagctctt acagtggttt ttttatcgat
     1081 aatgtagttt gcaagttcga ttgcccgctc ttcgatatat cccttcaact gtatataccc
     1141 ccctctttaa gtcatactat ggagaaatgc agtcattcgc tagtacatga aggcatagtc
     1201 agcctcttgt cttccaggac ccaaatcggg agaccactgt cattccatct gcagacgacc
     1261 tgaccggatc agcccgatat gcgtacgcag gccagacagt cctgcagatg ctgcgtacgg
     1321 tctttgcacg tctactgtat atatatgccc tgcatgacgc tcatatgagt taaaagggga
     1381 aaaaacaaaa acaacgttgc cggcggcggt aaaaccggac agaaaagcag acaggaatgc
     1441 cgaagatcct ccacacggat ctctcagcat ccctgtcccg attgcatcag tcttccgtca
     1501 tcctgcataa cctgtggtta tgcggtttta tactgtatca ttttatgggc aaataatcat
     1561 tcggatcaac gttttcatca tcaaccagca cctcgaagtg caggtgcggc tgttccgaag
     1621 attcatccat tgctgtgttc ccgatgctgc cgattatttc gccctggttt actttctggt
     1681 tcacagccac cgcctcatct gaagcaaggt tcctgtaaag cgttttcaga ccgtctccat
     1741 ggtcgataac gacggatatg ccgtagaact tgtcattgcg gacttcagat atgactccgt
     1801 cagccaccgc ctttacagga gtgcctctgt cggctgctat atcgataccc ggatggacgc
     1861 gccactgttc aagtgtcttg gagtacacca gcctgtccat tgcatattca aatgaaattt
     1921 ccccattgac aggcataatg aaactttgct tcacgggcgc tgattgcttc ttcgaatcac
     1981 ctttgccggg tgcctcgtct ttcggttccg tatctttttt actgctctca tcaggttcgc
     2041 cggaaagttc gccgtccttc gttaccgacg ccgggctgcc gatatcggat tcattctcac
     2101 caggcctgga aaccaattct tcatcgggat ccaggtccgt cagcttttca tctgtgttca
     2161 aggctgcatc ttcggctgga atatcgccca tgtcatcttc agcaatattt tcgctttcat
     2221 actcgggcgt tttgtccaaa gatcttcgtg aagtgacata caccgcggtt ccggccacta
     2281 cagctatgca cagcagcaga acgatataga aacccttctt gtccaggaac tccctgaatt
     2341 tattgccttt ttcatctgga aacgacttct tcatattcac aaattccacc tccgtatatt
     2401 attgttgccg aatatggcag gtatatacat acggtgtgaa attatgagga attattttca
     2461 taagatatgt gctgttttca gttcccggag tcccgggcgg cagcttcgag gtcggatatg
     2521 gatacgatgt cgatgcctgt ataataatgg gttagtattt ccctgaacgt ccctccggtc
     2581 tttgcaaggg catttgcgcc ccactggctc atacccacgc cgtggccatg gcctgtggtc
     2641 gttatgctta tcatgccgtc atcgccggat gcgatgctga aacatgcgga tctgaggccg
     2701 agcagcgacc tgaactcggt ccccttcatg gtaatattgc ctattttcag tgtttttact
     2761 cttccgcctt cagtaagatc gactattttt attccattgg caatatcttc aggcagttcc
     2821 gcatcgggcc aggcttccag caacttttcc gcaagttcgt ctccacttat ctttacggtg
     2881 gtgatatagc ccttcgaggc ctcgtcattg ctgaccacgc ttcggagata cggcacactt
     2941 ttgccgttcc acacatcctc ggcattttct gtccttccgg ggctgcttgc atggaaaagt
     3001 gcatttatga cattgccctg gtaaaccatt ataaggcctt tggtttcatc gacggcccgt
     3061 tctattttgt tccagtttct tgcagcaaac agcatgcccc atttttttct tgcattttcc
     3121 ttgctcaccc atgcctgaca gtgggtagaa tcggtgcaga tctctatgcc gtcatggact
     3181 ccctgttttg accggtaggc tccggtcatg cgtccgtaag caaaagtcct ggcagccacc
     3241 gcctgcgcct tgagcgcttc caggtcgaaa tctgccggca tttcagccgc caccacgcct
     3301 ttgatatact cttccagatc cattgtttcc gatgaaccgg tatccttgtt atagacggtt
     3361 atttcaaaca ctttttccgg tatctcggca gcaggcggct cctgcggcgg agccggctcc
     3421 gggctgcagc ttctgacaat gatcgcaggg agcagtataa cgataagcac cataacggat
     3481 gtataataaa agaacttctt catcaggacc cccgcggcat ggatatttta tgtcttctaa
     3541 tatatattca tattaaaaga aagtatgcag agggtaactg aaactttgtc cgcggcatgg
     3601 tccaacgctc cgtgccggta ctcgacggcg gtacggaaga agctccattc gctttcaaac
     3661 cttcactcgc cttcagccct gcttataaca gccccaaggt tt
//
LOCUS       DAOSCC010000227         3692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15892_length_3692_cov_4.496838, whole genome shotgun sequence.
ACCESSION   DAOSCC010000227 DAOSCC010000000
VERSION     DAOSCC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_3692_cov_4.496838"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLG72_10190"
     CDS             <1..291
                     /locus_tag="PLG72_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ81206.1"
                     /translation="IKATNELSKGLNRLMVLAESYPDLKANSNFQELMKELTKTEDKI
                     AFARQFYNDVVMDYNNSIQMFPKNILANAFNFMEEPFFTIDGNETKAPEVKF"
     gene            766..2745
                     /locus_tag="PLG72_10195"
     CDS             766..2745
                     /locus_tag="PLG72_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ81207.1"
                     /translation="MVKTAYKTDRPACEGCCCSAGARECRSGKQPLQAARADIAILAA
                     GIVLFAAALILRNYPTVSFILFFSAWMTAGYKVIYRSFTNIAKGRIFDENLLMTIATV
                     GAFLTGQYGEAAAVMLFYKLGSILEDMAVSHSRRSISELMALRPDHANLKQGDETIKI
                     APEDIKPGDILVVRPGERIPVDSVVVEGSSLADTSSLTGESLPKTVGPGDQVLAGYIN
                     KTGLLTVRADRALEESAVSRILELVEEAASRKAPMESFINRFAAYYTPVVTISALLIA
                     VIPPLVTGSMDFMTWLYRAMIFLVISCPCALVVSIPLTFYAGIGKSSANGILVKGGNY
                     LDALNKADTVVFDKTGTLTKGVFNVTGIRPYGCTEDELLRLAAYAEYNSSHPIARSIL
                     RAYGEDIDESLIKDSVETAGKGVRTNAIGHDIIAGNLEFIKSFVKTDSSGGTISSTDD
                     AIPGHDNDRAGTTVHVMSDGRYMGWIEVSDTLREDAAKAVDGLRKHGIKKVAMLTGDS
                     RVPAENAARELGLDEVGYGLLPHEKAGRLEKIMSAANGHTIFVGDGINDAPVLASADI
                     GVSMGGMGSDAAIEASDIVLMTDEPSRLADAMKIASITRKTAVQNIVFAISVKVLFLL
                     LGAIGAATMWEAVFADVGVTVLAVLNTLRIRHHRI"
     gene            complement(2840..3307)
                     /locus_tag="PLG72_10200"
     CDS             complement(2840..3307)
                     /locus_tag="PLG72_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81208.1"
                     /translation="MQDYYIIRQPQPPFGGMFGPSQPGQFFPPGQGPGFFPPGQGPAF
                     PPGQGPGFPPGQGPGLPPGQGPGTGPPPGRPPSFVPQQAQAQQTVGALAVDPGSIRRC
                     MFRYVYIWLDNGQQFWAWLVFVGRQSVAGWRWIGFRWVYFGIDLRRIVSFACF"
     gene            3485..>3692
                     /locus_tag="PLG72_10205"
     CDS             3485..>3692
                     /locus_tag="PLG72_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ81209.1"
                     /translation="MNGKYGVDLTTGSIPKHLVRFSIPMFIGNLLQTALGFTDAMWVG
                     NMVGPNAVGAIQVSFPIQFLLIALS"
BASE COUNT          956 a          964 c          977 g          795 t
ORIGIN      
        1 atcaaggcca ccaacgagct ttccaagggg ctcaaccggc tgatggtcct tgcggaatcc
       61 tatcctgacc tcaaggcaaa ctccaacttc caggaactca tgaaggaact caccaagacc
      121 gaggacaaga tagcattcgc ccgtcagttc tacaacgacg tcgttatgga ctacaacaac
      181 tcgatccaga tgttcccgaa gaacatcctc gcaaacgcct tcaacttcat ggaagagccc
      241 ttcttcacca ttgacgggaa cgaaacaaag gcgcctgagg taaagttcta gataccggtt
      301 tgggtaccgt gcccgggtgt cagccaatgg ctcattgaaa caggttgtaa tggaaacagg
      361 ggtataaaca gctcattgat ctgaaactga tgcaaatggg ctttaagcag tttcgtacgg
      421 ctttcgtgtc cgggctctgc aaactcagac acgggagccg tccttatgtc ccgcaggatc
      481 tgtcctgcta ttttgatcct gtctcatcga tatgccatcc cactgcttca cctgtgcctc
      541 atcagcatac cgtccgcaag tctcagccgc cccatttgta ccgctatcat accatatctc
      601 agccttccga cctgtcccgg tgcctgctgc tgtctgtcaa aaaatctgtt taaacctcat
      661 tgacctcatt gacaattcca cccatgcagg attataatta taacaaacat atgaataggt
      721 gttcatatgt gcaatactag tcggtgatat aaaggtggag ttgcgatggt caaaacagct
      781 tacaaaacag atcgtccggc atgcgaaggg tgctgctgtt cagcaggagc cagggaatgc
      841 agatccggta aacagccgct gcaggctgca cgagcggaca tcgcgatact ggctgcaggt
      901 attgtcttgt tcgcagcagc gcttatcctt cggaattatc cgacagtttc gttcatactg
      961 tttttcagcg cctggatgac agccggatat aaagtaatat acaggtcatt cacgaacatc
     1021 gcgaaaggca ggatatttga cgaaaacctg ctcatgacca tagctacggt cggagctttc
     1081 ctgacgggcc agtacggcga agccgcagct gtcatgcttt tctacaagct tggctcgatc
     1141 cttgaagaca tggctgtaag ccattcgagg cggtccatat cggagttgat ggctctcagg
     1201 ccggatcacg cgaaccttaa gcagggcgat gaaaccataa aaatcgcgcc tgaggacata
     1261 aaaccaggcg atatcctggt agtaaggccc ggggagagga tacctgtgga cagcgtcgtt
     1321 gtcgagggca gttcgctggc agacacctcc tccctcaccg gcgaatcgct gccaaaaacg
     1381 gtgggacctg gtgaccaggt tctcgcagga tacatcaaca aaacaggcct tttgaccgtc
     1441 agggctgaca gggccctgga ggagtccgca gtttcccgca tactggaact ggtcgaggaa
     1501 gcagcatcaa ggaaagctcc catggaaagc ttcataaacc ggtttgctgc gtactacact
     1561 cctgtggtca ccatatcggc attgctcata gctgttatcc cgccgctggt gactggaagc
     1621 atggatttca tgacttggct gtacagggcg atgatattcc tcgtgatatc atgcccgtgc
     1681 gcgctggtgg tatcgatacc gctcacgttc tacgcaggaa tcggcaaatc ttcagcaaac
     1741 ggcatcctgg tgaaaggcgg caattacctc gatgcactga acaaggctga tacagtggta
     1801 ttcgacaaaa caggcacgct gacgaaaggc gttttcaacg tgaccggtat aaggccttat
     1861 ggctgcacag aggatgaact gctcaggctg gcggcatatg ccgagtacaa ctcgtcgcat
     1921 cctatagccc gttctatcct tcgggcttac ggtgaagata tagacgagtc gctgataaaa
     1981 gacagcgtcg aaaccgcagg caaaggcgtc cggaccaatg ccatcggtca tgacataatc
     2041 gccggcaatc ttgaattcat aaagagtttc gtcaagacgg acagctccgg cggcacgatc
     2101 agcagcacgg acgatgccat cccgggccat gacaatgaca gggccgggac aacggtccac
     2161 gtaatgtcgg acgggcgcta tatgggctgg atcgaagtgt ccgacactct cagggaagat
     2221 gcggcaaagg cagtcgacgg gctcaggaaa cacggcatca aaaaagtcgc gatgcttacc
     2281 ggcgactcac gcgtccctgc cgaaaacgca gcgcgcgaac tcggtctcga tgaagtggga
     2341 tacggactgc tgccccatga aaaggcaggc cgtctcgaga aaataatgtc ggctgcgaac
     2401 ggacacacga tcttcgtcgg tgacgggata aatgacgctc ctgtgcttgc atcggcagat
     2461 ataggggtgt cgatgggagg catgggatcg gatgctgcca tcgaagcatc ggatatcgtg
     2521 cttatgaccg acgaaccttc gaggctggcg gatgcaatga aaatagcatc tattacgagg
     2581 aaaacagcag tccagaacat agtattcgcc atttcggtaa aagtgctgtt cctgctgctc
     2641 ggcgccatag gtgcggccac catgtgggag gcggtattcg cagatgtagg tgtgactgtg
     2701 ctggcagtgc tcaacacgct gcgcatcagg catcatcgta tataaaaagc gtcaggcggc
     2761 gtatgcaccc cgcctgacaa aaaacaactg atggaagggg gcttatggcc tggaataaaa
     2821 cactggaagt gactgaactc taaaaacatg caaatgatac tatccttctc aggtcaatac
     2881 caaagtagac ccatctgaaa cctatccatc tccatccggc gaccgactgt ctgccgacga
     2941 atacgagcca cgcccagaat tgctgtccgt tatcgagcca aatatagaca tatctgaaca
     3001 tgcaccgtct tattgaaccg ggatcgactg caagcgcccc gaccgtttgc tgtgcctggg
     3061 cctgctgcgg gacgaatgac ggcggacggc ccggaggagg tcctgtgcct ggaccctgtc
     3121 ccggcggtaa acccggtccc tggccgggcg gaaagcctgg cccctgtccg ggaggaaacg
     3181 ccggtccctg gccgggcgga aagaaacccg gaccctgtcc cggcggaaag aactggcctg
     3241 gctgggacgg gccgaacatg cctccgaaag gaggctgagg ctgtcttatg atataataat
     3301 cctgcatcgt gctcactcct tagtgtatct ggtatagcct atgcaggatc ggttataaag
     3361 gttacataaa aactctacac tatattattc aacagcgtat ttgttgcaac atcaaacaaa
     3421 taatgatatt gtaataaaaa ctggcgatat atctgtaaaa cacaaacaga taaacggagg
     3481 caatatgaac ggcaaatacg gagtcgacct gacgaccggc agtataccaa agcatcttgt
     3541 gcgcttttcg atacccatgt tcataggcaa tctgctgcag acagcgctgg gatttacaga
     3601 cgccatgtgg gtgggaaaca tggttggccc gaatgcggtc ggagccatac aggtcagctt
     3661 tcccatccag tttctgctga tcgcgctgtc aa
//
LOCUS       DAOSCC010000228         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15928_length_3685_cov_4.916253, whole genome shotgun sequence.
ACCESSION   DAOSCC010000228 DAOSCC010000000
VERSION     DAOSCC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15928_length_3685_cov_4.916253"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLG72_10210"
     CDS             <1..745
                     /locus_tag="PLG72_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOJ81210.1"
                     /translation="SIVPPGVTIINGCIYRSCEEKDGYLSVRFCRDGREYEERTKLLV
                     GADGAFSGVRRQFFGNLPSPKLYICVQEWYRTDGGRNYYGAVFDDDLTDFYSWTIPKE
                     DRIILGAALPPRRDVLLKFEELKGKLRDYGFDFSERLARNGAYLYRPVRSSQICTGGD
                     RIALVGEAAGFISPSSAEGISYAFRSALALARALEDGIEGFAARYAKNLQPLKRNIFL
                     KNMKCPAMYNKDLRRLAMKSGLLSIDIVE"
     gene            954..1349
                     /locus_tag="PLG72_10215"
     CDS             954..1349
                     /locus_tag="PLG72_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ81211.1"
                     /translation="MFEHIVKTSSSDEMINITSLVRQDVQKSGITDGIAVVFVPHTTA
                     GVTINENADPDVVYDLLGILRRAFPESGSYRHMEGNSHAHARASVVGSSCTVIIENGQ
                     LKLGTWQGIYFCEFDGPRTRKVYVKLIQG"
     gene            1487..1639
                     /locus_tag="PLG72_10220"
     CDS             1487..1639
                     /locus_tag="PLG72_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81212.1"
                     /translation="MKKKKTMYDLMKEIRKTWSINPKTRVREDETKNKKKRRQQEKKM
                     IRDESP"
     gene            1636..2208
                     /locus_tag="PLG72_10225"
     CDS             1636..2208
                     /locus_tag="PLG72_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOJ81213.1"
                     /translation="MMIEAVIKGLGFKFETSDNVFSPRKVDAGTMAMLSFVEFQDGWK
                     VLDLGCGYGVVGITAARFTDPAKVYMVDIDPEAVSLARANAARNGVGNVNILRSDGFD
                     ALDETGFDCILSNPPYHTDFSVAKRFIEKGFNRLKIGGKMYMVTKRKDWYKNKLISIF
                     GGVRIHETDGYYVFEAEKRSASYAGKSRSK"
     gene            complement(2216..>3685)
                     /locus_tag="PLG72_10230"
     CDS             complement(2216..>3685)
                     /locus_tag="PLG72_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ81214.1"
                     /translation="GKVPAVETLKELADRLSFYKINQLQLYIEHSFAFRKHSEIWTDS
                     DPLTAEDILILDEYCRRRSVELVPSLSSFGHLYHALVSKSFRHLNEYDDIPDRPFTWT
                     DRMAHYTLDASAPESLGFVRDMIDEFIPLFTSDKFNICCDETFDLGKGKNSTLAAEIG
                     EGKLYLYFVRGLIEHLKSHKKKVMMWGDMLLNYPEIIGEIPKNTVILNWNYAADAAED
                     GFRKISEAGLKQYVCPGVQGWNRLLNDIDTATANIDKLAGYAKTCGAVGMLNTDWGDF
                     GHINVLAGSIPGAIYGAGLSWNLDRPGQPTDEEISVMEYGDRSGKIVGLIRELSRQPV
                     MDWYDVVLWYYASCGYDTGAYGSADHIRERLLGLDAAAARSAYGRIRELSGEISSLLG
                     NIYENRRTDIREVICAADGLALFQALLPVIRKELLDQASEFPGRECAEPIFSPGELAV
                     KLEYWFSDYKAVWRTRNRESELFRIKEVVMGVCRLLREI"
BASE COUNT          869 a          912 c         1035 g          869 t
ORIGIN      
        1 atccatcgtt ccccccggcg tgacgataat aaatggctgc atatacaggt catgcgagga
       61 aaaggacgga tacctgtcgg tcaggttctg ccgggacggc agggaatacg aagagaggac
      121 gaaactgctg gtgggcgccg acggcgcgtt ttccggcgtg aggcggcagt ttttcgggaa
      181 cctgccgtcg ccaaagcttt acatatgtgt ccaggaatgg tacaggacag acggcggcag
      241 gaattattac ggcgctgttt tcgacgatga tctgaccgac ttttactcat ggaccatacc
      301 gaaagaagac cggatcatac tcggggctgc cctgccgccc cgcagggatg tgctcctgaa
      361 gttcgaggaa ctgaaaggca aactgaggga ctacgggttt gatttcagtg agcgccttgc
      421 caggaacggc gcatacctgt acagacctgt caggagcagc cagatttgca cgggcggcga
      481 caggatcgcc cttgtgggcg aggctgccgg atttataagc ccgagttcgg cagaggggat
      541 cagctatgcg ttcaggagcg ccctggcgct ggcgcgggcc ctggaagacg gcatagaggg
      601 ctttgcggcc aggtatgcga agaacctgca gcccctgaaa aggaacatat tcctgaaaaa
      661 tatgaaatgt cccgcaatgt acaataagga tctcaggaga cttgccatga aatcggggct
      721 gctgtccata gatatagtgg aatgatcctg ttattctatc atggagcgac atatcgttcc
      781 ttcagtgccg gagggtgtgc cacgatcacc tttttcccga ccgacaaggg aaccgatgtt
      841 ctctctctta tattccagct gcctgttttt aattttatgt gataatatgt taatattgcg
      901 tgtacagtac tgcgatatta agatatggga aaattacaga aagggcagag gttatgttcg
      961 agcatattgt caaaacgtct tcatctgacg agatgatcaa tattacgtca ctggtcaggc
     1021 aggatgtgca gaaaagcggg atcaccgacg ggatagctgt tgttttcgta ccccatacta
     1081 cagcgggcgt caccatcaat gagaacgcgg atccggatgt cgtttatgac ctgcttggca
     1141 ttttgcgcag ggcatttccc gaaagcggga gttacaggca tatggagggc aattcccatg
     1201 cccatgccag ggcttcggtg gtcggctcgt cctgtacggt tataatagaa aacggccaat
     1261 tgaagcttgg aacatggcag gggatctatt tctgcgaatt tgacggccca aggaccagga
     1321 aagtttacgt aaagctgata caggggtaaa tgacggaggc ttgcgcgagg gcgatccagg
     1381 ctgatatacg ggcagccggg ccgatactgc agcgggcctg atacaggggc agcctggtat
     1441 ttgctgctgc agccgggacg gatactgctg taccggggag atagttatga agaaaaagaa
     1501 gaccatgtat gacctgatga aagagatcag gaaaacctgg agcataaacc cgaagaccag
     1561 ggtccgggag gatgagacaa aaaacaaaaa gaaacgcagg cagcaggaga aaaaaatgat
     1621 acgggatgag tcgccatgat gatcgaagct gtgatcaaag gcctgggatt caaatttgag
     1681 acttcggaca atgtgttttc accgaggaag gtagacgccg gcacaatggc gatgctgtcc
     1741 ttcgtcgaat tccaggatgg atggaaagtg cttgaccttg ggtgcggata cggagtcgtc
     1801 ggcatcacgg cggcaaggtt cactgatcct gcgaaggtgt acatggtcga tatcgatccc
     1861 gaggctgtat cgctggccag ggccaatgcg gcaagaaacg gcgtgggaaa cgtgaacata
     1921 ctccggagcg atggttttga cgcattggat gaaaccgggt tcgactgtat tttgtctaac
     1981 cctccttacc atacggattt ttccgtggcc aaaaggttta tagaaaaagg attcaaccgt
     2041 ctgaagatcg gcggaaaaat gtacatggtc acaaagagga aggactggta caaaaacaag
     2101 cttatcagta tcttcggcgg cgtgcggata catgagaccg acgggtatta tgttttcgaa
     2161 gccgaaaaaa gaagcgcaag ctatgccggg aaaagcagaa gcaagtgatg cgcggtcaga
     2221 tttccctcag cagcctgcat acgcccatga ccacttcttt tatcctgaag agttcgcttt
     2281 ccctgtttct ggtacgccat acggctttgt agtcggagaa ccagtactcg agttttactg
     2341 ccagttcacc ggggctgaat attggttcgg cgcattccct tccagggaat tcggacgcct
     2401 ggtccagcaa ttcttttctt atcacaggga gcagcgcctg gaagagcgcg agcccgtccg
     2461 cagcgcaaat tacctccctg atatcggttc tcctgttttc atatatgttc ccgagcaagg
     2521 acgatatctc gcctgagagc tcccttatcc tgccgtaggc tgatcttgct gccgcagcgt
     2581 caagaccgag cagcctttcc ctgatatgat ccgcactgcc gtatgcgccc gtatcatatc
     2641 cgcaggaggc ataataccac agcaccacat cataccagtc catcaccggc tggcgggata
     2701 gctcccgtat caggccgacg atctttccgg accggtcccc gtattccatt accgatatct
     2761 cttcatccgt tggctgtccg ggcctgtcca ggttccatga gaggcctgcg ccgtatatgg
     2821 cgcccgggat ggagcctgcc agcacattta tatggccaaa gtcgccccag tccgtgttca
     2881 gcatgccgac cgcaccgcag gttttggcgt atccggccag tttatctata ttggccgttg
     2941 ccgtgtcgat gtcgttcagc agcctgttcc agccctgcac gccggggcag acatactgtt
     3001 tcaggccggc ttcggatatc ttcctgaatc cgtcttcagc ggcatccgca gcgtaattcc
     3061 agttgagtat caccgtgttt ttgggtatct cgccgattat ctccggatag tttagcagca
     3121 tgtcgcccca catcatgact ttctttttgt ggctcttcag gtgttctatc aggcccctga
     3181 caaaatagag gtacagcttg ccttctccta tttctgcggc cagggtgctg tttttccctt
     3241 tgccgagatc gaaggtctcg tcgcagcaga tattgaactt gtcggaggtg aacagcggta
     3301 tgaactcgtc tatcatatcc ctgacgaacc ccaggctctc cggggcgctc gcgtcgaggg
     3361 tgtaatgcgc catccggtcg gtccacgtga acggcctgtc gggtatatcg tcatattcgt
     3421 tgaggtgcct gaatgacttt gaaaccagcg cgtggtacag gtggccgaat gatgacagcg
     3481 acgggacgag ttctacggac ctcctcctgc agtactcgtc cagtataagt atatcttcgg
     3541 ccgtcagcgg atctgaatcg gtccatatct cgctgtgttt gcggaaggca aacgaatgct
     3601 ctatatagag ctgaagctgg tttatcttgt agaaggacag cctgtctgca agttccttca
     3661 aagtttcgac ggccggtacc tttcc
//
LOCUS       DAOSCC010000229         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_15976_length_3675_cov_3.716575, whole genome shotgun sequence.
ACCESSION   DAOSCC010000229 DAOSCC010000000
VERSION     DAOSCC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15976_length_3675_cov_3.716575"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLG72_10235"
     CDS             complement(<1..623)
                     /locus_tag="PLG72_10235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ81215.1"
                     /translation="MIDSVCIFGDSIMKGVIFDGIKGKYTYLKNSFLNILRGYNSSVR
                     VDNFAKFGCTITVGQKIIEKHADKLSGYRYTALEFGGNDCDFDWKKISERPDDMHLPK
                     TPLEEFESLYGRIIDDISQAGSRPVLFSLPPLSAERYFAWISKGLNAENILRWLGDVG
                     HIYRWHEMYNNAVLKIAAAKNAMLIDIREAFLKTRDYMNLLCEDGIHP"
     gene            866..1588
                     /locus_tag="PLG72_10240"
     CDS             866..1588
                     /locus_tag="PLG72_10240"
                     /inference="COORDINATES: protein motif:HMM:NF020450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HOJ81216.1"
                     /translation="MEMIDQHETDDMEQKTNDLKEKLGQLKQKLASERPVKWKEFPDI
                     GLYKDQVVSYMNRQLINFEEGGQLTSAMVNNYIKDGLLPRADGKKYSREHLAGLTEIC
                     LLKQVLSVKDTGFLLKYVQKGKDNETFYTNFIEVLDRALADTAGRLDAEWNMEALPDM
                     ALKLAISSYCDKLACERLIRIMQQAEEQEIQKKPDKKHDRRQESKAGSKAGPEADKTA
                     DKKEIEKTDQRPGQNSGKKRSV"
     regulatory      1791..1919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1931..2749
                     /locus_tag="PLG72_10245"
     CDS             1931..2749
                     /locus_tag="PLG72_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOJ81217.1"
                     /translation="MSRKKKHLASLSAFMLAAILSAQSAFAYSGTVLKKGMRSDEVYA
                     LQEDLYELGYMKVKPTGYYGSITEAAVLKLQKEYGLLVDGIAGKQTKGKLDSLMGRDG
                     ASADAAALSAASVAETASENPSAASEGASEAAAGKSSEKTSRSASRAADDIISYAKKY
                     LGVKYVWGGSTPKGFDCSGFVKYVYEHFDVSLSRTSASQAKNGTKVNKADLKPGDLVF
                     FDTDGGRNRINHVGIYIGNGKMIHSSSHFGGVVITDISGGFYAKSYMTARRVLK"
     gene            3012..>3675
                     /gene="sbcD"
                     /locus_tag="PLG72_10250"
     CDS             3012..>3675
                     /gene="sbcD"
                     /locus_tag="PLG72_10250"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOJ81218.1"
                     /translation="MRILHTSDWHLGRSLEQISRLEEQREFIDSLVRIADKEDVDLVL
                     VAGDIYDTYNPSAAAEELFYEAMDRLGGKSGRAVIVIAGNHDNPDRLCAASPLAYKNG
                     IILLGYPGSDAGEYRMGAGGINITDSGPGWLELSLPRCGHNAVIITMPFPSESRLEEL
                     LSEKADEAELQNAYSEKIGSIFAGLSVKFRDDTVNLIVAHLFLLGGSTSDSERTLQVG
                     GAM"
BASE COUNT         1036 a          822 c          958 g          859 t
ORIGIN      
        1 ggatggatgc cgtcttcgca cagcaggttc atataatccc tggtcttcag gaacgcttcc
       61 cgtatatcta tgagcattgc gtttttagct gcagcgatct tcagcaccgc gttgttgtac
      121 atctcatgcc acctgtagat atgtcccaca tccccgagcc agcggagtat attctcggca
      181 ttcaggcctt ttgagatcca tgcgaaatac ctttcagcgc tcagcggcgg caatgagaac
      241 agcacgggcc tgcttcctgc ctgtgaaata tcgtcgatga tcctgccgta cagcgattcg
      301 aattcttcca gcggtgtttt tggcagatgc atatcgtccg gacgctcgga tatctttttc
      361 cagtcgaaat cgcagtcgtt gccgccgaat tcgagcgcag tgtacctgta gcctgaaagc
      421 ttgtcagcat gcttctctat gattttttgg ccgacagtga tcgtgcatcc gaactttgcg
      481 aagttatcta ccctgactga actgttgtat cctctcagga tattcaggaa actgtttttc
      541 aggtaagtgt acttgccctt gatgccgtcg aatataacgc ctttcattat ggaatctccg
      601 aatatgcata cagagtctat cattttatgt cacctgttct taaataatat ttaatctaat
      661 attgaatatt atattgtgtg ataatacata tgtcaatata cacatggtaa atcatctacg
      721 acgtttgcat tatgacgtta tgtgtagtaa aatattaaga aaacatgtcg aaaagcaatc
      781 gtgtactgca aatatgcgtt ttgatggagc ttcaccgttt atcgaagctg tattttttga
      841 aaaacggatg gtccgggagg ctgtcatgga aatgatcgat cagcatgaaa cagatgatat
      901 ggaacagaaa acaaatgatc tgaaggaaaa actggggcag ctgaagcaga agcttgcctc
      961 agagcgtcct gtcaaatgga aggaatttcc cgatatcggc ctgtacaagg accaggtcgt
     1021 atcgtatatg aacaggcagc tgataaattt cgaggagggc gggcagctga cctcagcaat
     1081 ggtcaacaac tatataaagg acggcctgct gccgagggca gacggcaaga agtattcccg
     1141 cgagcacctg gcgggactta cggagatatg cctgctcaaa caggtactgt cggtgaagga
     1201 caccgggttc ctgttgaagt atgtccaaaa gggcaaagat aatgaaacat tttatacgaa
     1261 cttcattgaa gttctcgaca gagcactggc cgataccgca ggcaggctcg acgcggaatg
     1321 gaacatggag gctttgcctg atatggcatt gaagctggcg atatcaagtt actgcgacaa
     1381 gctcgcctgc gaaaggctta taaggataat gcagcaggct gaagagcagg aaatacagaa
     1441 aaagcctgat aaaaaacatg accgcaggca ggaaagcaaa gctggaagta aagcaggacc
     1501 tgaagcggat aagacagctg ataaaaaaga gatcgaaaaa actgatcaga gaccaggtca
     1561 gaactccggc aaaaaaagat ccgtctaata ggtggaccgt aatggatgtt tgatcctggt
     1621 ccaccggaaa tcatcgtttc tgatccatcg gaaatgatcg tccctgattt ccgtcggtcc
     1681 tgaactaaaa aaccatcatg gcttctgttg atatacacag gatatctgtc gacatatctt
     1741 cctctttatt gtaaattatt ataggtttcc ataatacctt cattatggta aacttaattg
     1801 tgccgaatcc tttaggggag cgggggagtc aattttttgg ggtgaagcca ttacacatgg
     1861 cggggtgtac tcttttacct aacccgtcag ctaacctcgc aggcgaaaag aaaagaggag
     1921 gatacataaa atgtcacgta agaagaagca tctggcatcg ctgtctgcgt tcatgctggc
     1981 tgccattttg tctgcacaat cggcgtttgc ctattccgga acggtactga aaaagggcat
     2041 gagaagcgac gaagtatatg ccctgcagga agacctttac gaactggggt acatgaaagt
     2101 caagccgaca gggtattacg gaagcatcac tgaggcagcc gtactgaaac tgcagaagga
     2161 atacggcctc ctggtggacg gtatcgccgg gaagcagacg aaaggcaaac tggacagcct
     2221 tatgggcagg gacggggcat cggccgatgc tgcagcgtta agcgcagcaa gtgtggcaga
     2281 gaccgcatcc gaaaatcctt cggccgcatc agaaggcgca tctgaggctg cagccggaaa
     2341 gtcatccgaa aaaacttcca ggagcgcatc gcgggcggct gatgatatca tcagctacgc
     2401 taaaaaatac ctcggtgtga aatacgtatg gggaggatcc accccgaaag gttttgactg
     2461 ttcaggcttt gtaaagtatg tttacgaaca tttcgacgtc tctttgagca ggacttccgc
     2521 atcccaggct aagaacggca caaaggtgaa caaggcggac ctgaagcctg gagacctggt
     2581 atttttcgat accgacgggg gccgtaaccg cataaaccat gtcggcatct atataggaaa
     2641 cggaaaaatg atacacagtt cctcccattt cggaggtgtc gtaataacag atataagcgg
     2701 cggattttac gcaaagagct atatgactgc aagaagggta ctgaaataac agaaagtctt
     2761 cctggcagac aagcatccaa aacagccggg atcctccgga aatccggaaa ggctgcgtaa
     2821 aagcaaccgg cgggcaattg cagacagtga aagcaaagca aaaaggcaga ggagcaaata
     2881 tcaggaatac tgcacagaag tacagacctc acggtgtcgg acgataccgt gaggttcttt
     2941 tatgcgtctg ataaaatgtt ataatcaata tatacggttc agtggtgtct ttatgcggga
     3001 ggtgacgcct gatgcgaatc ttacatacat ccgactggca tcttggcagg agcctcgagc
     3061 agatcagcag gctcgaggaa cagcgcgaat tcatcgacag ccttgtaagg atcgccgaca
     3121 aagaggatgt ggatctcgtc ctggttgcag gggacatata tgatacgtat aatccttcgg
     3181 ccgccgctga agaactgttc tacgaggcta tggacaggct gggcggaaaa agcgggagag
     3241 ccgtcatcgt catcgccggc aaccatgaca atcctgacag gctttgcgca gcgagcccgc
     3301 tggcatataa aaacggtata atccttctcg gttatccggg aagcgacgcc ggcgaatata
     3361 gaatgggcgc cggaggcata aacatcaccg attcaggccc cggctggctg gaactcagcc
     3421 tgccgcgatg cggccacaat gcggtcataa taaccatgcc gtttccttcc gaatccaggc
     3481 tggaagaact gctttctgaa aaggcggatg aggctgaact gcagaatgct tattctgaga
     3541 agatcggcag tatctttgcc gggttgtccg ttaaattcag ggacgacaca gtcaacctga
     3601 tagttgctca tttgttcctg cttggcggca gcacatccga ctcggagagg actttgcagg
     3661 tgggaggcgc tatga
//
LOCUS       DAOSCC010000230         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_16087_length_3657_cov_4.268462, whole genome shotgun sequence.
ACCESSION   DAOSCC010000230 DAOSCC010000000
VERSION     DAOSCC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16087_length_3657_cov_4.268462"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1020)
                     /gene="secF"
                     /locus_tag="PLG72_10255"
     CDS             complement(64..1020)
                     /gene="secF"
                     /locus_tag="PLG72_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOJ81219.1"
                     /translation="MVDLMGKKYYFFALSLVILIVCIVGFLANGVVLDIQFQGGTIMQ
                     IEMPDDNFDTELAADIVKNAIGKTATVNKSSTFNAENEDERIYLMVLNIASEETLTED
                     ERTAVIDALREEFSIAEDASINVNSVEPFIGEEFRMNAVKAIVISSILIVLYVWLRFR
                     SMHGLSAGVFAVIALVHDIMVMFTAYVIFRIPLNDSFVAAMLTIIGYSINDTIVIYDR
                     IRENTKLMRKASYRELVNKSVLQCLTRSINTSLTTLIAVLTLLVFAVIYNIQSIREFT
                     MPLVVGLISGVYSTLFIASPLYMMWQERKLNRKAAAQKPAKT"
     gene            complement(1023..2339)
                     /gene="secD"
                     /locus_tag="PLG72_10260"
     CDS             complement(1023..2339)
                     /gene="secD"
                     /locus_tag="PLG72_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOJ81220.1"
                     /translation="MKGNNGLKLFLVLALIAVMTYICFAGSLFGLVEIPGVNDIVPGI
                     DINGGIDAMLYAITEDGRKPTQEELDTAKIIIEKRLDKEGILDRNVTTDLNAGRIIVQ
                     IPWAPGETDFNPDKTLEKIGRTALLTFQEVDEDKLDEDGNPLPTGRKVLEGTDVVDAY
                     PAKNTQTGNMEVVLELNDRGKESFAEATGRLIGQRIAIFMDDQFISAPVVQSHITEGI
                     AVIVLYKGSTKEGVQEAKDLADTIRAGALPFKMETKHVNAISPLLGKGALNVTVRAFI
                     VAFLLICTFMILYYRLPGLIASIALFMLAIMTLNFVSWLGITLTLPGLAGIVLSVGMG
                     VDANVIIFERIKEELRSGKTIKAAIDLGFKRAFSAIFDGNMTTVIVAVILWRFGTGAM
                     ISFAYTLIIGVVLSFFTALTLTKSMLEAVSDIDIARKTWLYGVKEA"
     gene            complement(2443..3120)
                     /locus_tag="PLG72_10265"
     CDS             complement(2443..3120)
                     /locus_tag="PLG72_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ81221.1"
                     /translation="MERGVTLVTVEDVRKNREVRTFLTIAETQMRVLGYTEHSFRHVG
                     LVSENAGKILEQLKYPEREVELARIAGYLHDIGNAVNRVDHAHIGALMAYRILTGMGM
                     DCEEAAQVMMAIGNHDEHTGTAVSSISAALILADKSDVHRSRVTNTDMATFDIHDRVN
                     YAVEKSVITVDSEKKTCDLVLKIDNKICPVMDYFEIFLVRMTMNRRAAAFLGLQFQLF
                     INDSKLI"
     gene            complement(3149..>3657)
                     /locus_tag="PLG72_10270"
     CDS             complement(3149..>3657)
                     /locus_tag="PLG72_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ81222.1"
                     /translation="KDTGFDIRREDLPVLFREYEDLALEYVRRHKESRGFNFFHFMID
                     LDHGPCVIKRMKGCGSGHEYLAVTPEGDLYPCHQFVGMDRFKMGNVNESFDIDTSISD
                     IFRKNNIYTNPECRKCWARFFCSGGCAANAWQFNNSLDKPYDIGCELERKRVECALWI
                     RAVESGME"
BASE COUNT          860 a          971 c          771 g         1055 t
ORIGIN      
        1 gtaccggttt ttgcggataa atgcctgttt atatggaatg tgttactgag tacattttct
       61 gcatcatgtt tttgccggtt tctgcgcagc tgccttcctg ttcagtttcc gctcctgcca
      121 catcatatac agcgggcttg cgatgaacag cgtcgaataa acgccgctta tgagaccgac
      181 cacaagaggc atcgtaaact ctctgatcga ctgtatgtta tatatcactg cgaataccag
      241 cagagtcaat accgcgatca acgttgtaag ggaagtattg atcgaccttg tcaggcactg
      301 gaggacgctc ttgtttacca gttccctgta agacgctttg cgcatcagtt tggtattctc
      361 ccttatcctg tcataaataa ctatcgtatc gttgatggaa taccctatga tcgtcagcat
      421 ggcagcaaca aacgaatcat tgagcggtat cctgaatatc acatatgccg tgaacatgac
      481 cattatatca tgtaccagtg caattacggc gaaaacacct gccgacagtc cgtgcatcga
      541 cctgaatctc agccagacgt acaggacgat cagtattgat gagatcacta ttgcttttac
      601 tgcattcatc ctgaactctt caccgatgaa aggttcaaca ctgttcacat tgatgcttgc
      661 atcttctgcg atgctgaatt cttccctcaa tgcgtctata accgccgtgc gctcatcttc
      721 ggtcagcgtt tcttcgctgg cgatattcag aaccatcagg tatatccttt catcttcatt
      781 ctcggcattg aaagtggaag acttgtttac tgtggctgtc ttgccgatcg catttttgac
      841 tatatcggca gccagttccg tatcaaagtt atcgtcaggc atttctatct gcatgatggt
      901 cccgccctga aactgtatgt ccaatactac gccatttgcg agaaaaccga ctatgcaaac
      961 aatcagtatg actaatgaca atgcaaaaaa gtagtatttt ttccccatca aatctaccat
     1021 ggctacgcct cctttacgcc gtacagccat gtcttcctgg ctatatcgat gtcagaaacc
     1081 gcttccagca ttgatttcgt aagcgtcaga gcagtaaaga agcttaggac gacgcctatg
     1141 atcaatgtat aggcgaacga tatcatcgcg cctgtaccaa atctccagag tataacagcc
     1201 acgataacgg tggtcatatt tccgtcgaat attgcggaaa atgccctctt gaatccaaga
     1261 tctatggcag cttttatagt cttgccggag cgcagttctt ccttgatcct ttcgaatatg
     1321 atgacattcg catccacgcc catgcctacc gacaggacga taccggcaag gccgggaagc
     1381 gtaagagtga tcccgagcca ggatacgaaa ttgagcgtca ttatcgcaag catgaaaagt
     1441 gctatggaag ctatgagtcc gggcaacctg taatacagta tcatgaatgt gcatatcaac
     1501 aggaatgcaa ctatgaatgc ccttacagta acattcagcg cgcctttgcc gagcagcggg
     1561 ctgatggcat tgacatgttt tgtctccatt ttgaacggaa gtgctccggc ccttatcgta
     1621 tcagccagat ccttggcctc ttgaactcct tctttggtgc tgcccttgta gagaactatc
     1681 acagctatac cttccgtgat atgtgactga acgaccgggg cggaaatgaa ctggtcatcc
     1741 atgaagatcg ctatacgctg tcctatcagc cttcctgtag cctctgcgaa cgattccttt
     1801 cccctgtcat tgagttccag gacaacttcc atgttccctg tctgcgtatt tttcgccgga
     1861 taggcgtcca cgacatcagt gccttccagt acttttcttc cagtgggaag cgggttcccg
     1921 tcttcatcca gtttatcttc atcaacttcc tggaatgtca gcaaagctgt ccttcctatt
     1981 ttttcgagtg tcttgtcagg gttgaagtct gtttccccgg gagcccatgg tatctggact
     2041 attatccttc ctgcattaag gtcggtagtg acgtttctgt cgagtatgcc ttccttgtcc
     2101 agccttttct cgataattat ctttgcagta tcgagttcct cctgtgtcgg ctttctgccg
     2161 tcttcggtga tcgcatacag catcgcatct attccaccgt tgatgtcgat gcccggaact
     2221 atatcattga cgccgggtat ctccaccagg ccgaaaagac tgcccgcgaa gcaaatatat
     2281 gtcatcacgg caatcaaagc gagaacgagg aacagtttca gcccgttgtt ccctttcatc
     2341 tgtaaaaaat ccccctttgc tttaaccaca tgtggtaatt atattactat cgtccaggcc
     2401 agtcaatatg aaagcttttg caataatgcc cgccgcaggc ggctaaatca gcttcgaatc
     2461 atttatgaac agttggaact gcaggccaag aaaagcagca gcccgtctgt tcatcgtcat
     2521 acgcaccagg aatatctcaa aatagtccat tacagggcat attttattat ctattttcaa
     2581 aaccaggtcg caggttttct tttcgctgtc tacggtaata accgattttt ccacggcgta
     2641 attgaccctg tcatggatgt cgaaagtggc catatcggta ttcgtcaccc tgctcctgtg
     2701 cacatctgac ttgtcggcaa gtatgagggc tgctgaaatg ctgctgacag ccgttcccgt
     2761 atgctcatca tggttcccta tcgccatcat cacctgtgcg gcttcttcac agtccatccc
     2821 catgcctgta agtatccggt aagccattag ggctccgatg tgggcatggt cgaccctgtt
     2881 gacggcattt cctatatcat gcagatatcc ggctatcctc gcaagttcca cttccctttc
     2941 gggatattta agctgttcca gtattttccc ggcattttcc gaaacaagcc ccacatgcct
     3001 gaaagagtgt tccgtgtaac ccagcacgcg catctgggtc tcggcaattg taaggaaagt
     3061 cctgacttcc ctgtttttcc tgacatcctc gacggtaacc aacgttacac ctctttccat
     3121 tcctgtattt ttccgctctt tggtccgttt attccatgcc ggattcgacc gccctgatcc
     3181 acaatgcaca ttccactctt tttctttcga gttcgcagcc tatatcataa ggcttgtcca
     3241 ggctgttatt gaactgccat gcattggcgg cgcagcctcc gctgcagaaa aacctggccc
     3301 agcatttcct gcattccgga ttcgtataaa tgttattctt tctgaagatg tcgcttatcg
     3361 aagtatcgat atcgaaactt tcattgacat tgcccatttt gaaacgatcc atgccaacga
     3421 actggtggca tgggtacagg tcgccttccg gcgtcactgc caggtactca tgccccgaac
     3481 cgcatccttt catcctcttt atcacgcacg ggccatgatc caggtcgatc atgaagtgga
     3541 agaagttgaa tcctctggat tccttatgcc ttctgacata ctcaagcgca aggtcttcgt
     3601 attccctgaa cagaaccgga agatcctccc gcctgatgtc gaaccctgta tccttcg
//
LOCUS       DAOSCC010000231         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_16192_length_3640_cov_4.447699, whole genome shotgun sequence.
ACCESSION   DAOSCC010000231 DAOSCC010000000
VERSION     DAOSCC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16192_length_3640_cov_4.447699"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLG72_10275"
     CDS             <1..238
                     /locus_tag="PLG72_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOJ81223.1"
                     /translation="ELGTMFEMTFIIKIFISAAVLAVGHVFNFAINALGAYVHSSRLQ
                     YLEFFGKFFTGGGQPFRPLKPNTKYITLKHDADT"
     gene            302..751
                     /locus_tag="PLG72_10280"
     CDS             302..751
                     /locus_tag="PLG72_10280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOJ81224.1"
                     /translation="MKIFDGQMIALLGAAFGFLMAGIGSSKGVGTAGQVGAGVLTEDP
                     SKFVSVMVLEALPSTQAIYGFVIAFFIIGRIDPAMTIAEGLHLFAAALPIGIVGFISA
                     IYQGKVASAGIQLIAKHSGSLVSAITLALMVEMFAILSLITSILMLG"
     gene            848..1501
                     /locus_tag="PLG72_10285"
     CDS             848..1501
                     /locus_tag="PLG72_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOJ81225.1"
                     /translation="MTGIDRIKEKILEDAKLKARATLEQAEQEARDIMEQAEQEAAQK
                     RAELLDKAKADGGQMYRRMLAVAGLEGRKKLLAARQEMIDEAFSKALERIEGLPDRQY
                     QELLEKMISEAAAGYRDPSAQPDGAHAPQAGFIAEIMLNERDAARMDGQFIENINRRL
                     SALGKKGTVTLSEKRIRTAGGFILKIGDVEMNSTFEILFGMLRTELESDVVGILFGS"
     gene            1558..2568
                     /locus_tag="PLG72_10290"
     CDS             1558..2568
                     /locus_tag="PLG72_10290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOJ81226.1"
                     /translation="MLRINEGDYSYASGLIRAKETKLLTDSQYARMLDASSAEEAYKV
                     LTDAGYGLGSGSTHSVFAFEQLLADEMKKCFMLLEEIAPKADVIRIFQKRYDYFNIKV
                     LLKAELSGQDIPPILADTGTIGSDSITRMIRERDYSGLTPSMADAVTQVYDVFARMQD
                     PQVVDLILDKASYQEFVADIRNIESPFLQELSDLIVDMTNIRMFIRSRSLDKTWDFIM
                     KLLIDGGTLDRDVYYRNSDKPVDSFVEDIRKSRYGDAVRKGLDLVKSGKNSSGLEKAL
                     DDLLMSYIRSAKLVTIGVEPLIAYLFAKETEIRNARMIMTGKINNLPPDMIRERLRES
                     YV"
     gene            2561..2878
                     /locus_tag="PLG72_10295"
     CDS             2561..2878
                     /locus_tag="PLG72_10295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOJ81227.1"
                     /translation="MYKIGVIGEKDAVLGFIALGFSVFPAENSEQAAETLRKLAQDRY
                     AVIYITEQTAAGIEQEIDKYRESRFPAVIPIPGIQGSLGIGMNGIRKCVEKAVGADIL
                     FRE"
     gene            3004..>3640
                     /locus_tag="PLG72_10300"
     CDS             3004..>3640
                     /locus_tag="PLG72_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ81228.1"
                     /translation="MSQGRIIKVSGPLVIAEGMRDANMFDVVRVGEKQLIGEILEIHG
                     DRASIQVYEETSGLGPGSPAVSTGEPLSVELGPGLLENIYDGIQRPLEVMRQTVGSNI
                     TRGINIPALDRSRKWPFKPAVSRGDMVVPGDILGTVQENVIFEHRILVPVGVEGVVED
                     ISEGEFTVEETVAVIKTPDGTSRNLTMMQKWPVRKERPYKQKLAPDEPLVTG"
BASE COUNT          940 a          846 c         1113 g          741 t
ORIGIN      
        1 tgaactgggt accatgtttg agatgacgtt tattataaag atattcattt ccgcagctgt
       61 cctggctgtc ggacatgtgt tcaattttgc aataaacgcg ctgggtgcat atgtccattc
      121 cagccgtctc cagtatctcg aatttttcgg caagtttttc acaggcggag gccagccctt
      181 cagacctttg aaaccaaata caaaatacat cacactaaag catgacgcgg atacctgagc
      241 ctgtcgcccg gacgggcatt ggagccgctc ggagaaaaat caggatggag gtaggaaaaa
      301 gatgaaaatt ttcgatggac agatgattgc tttgctggga gccgctttcg gattcctgat
      361 ggcaggtatc ggatcgtcaa aaggcgtcgg tactgctggt caggtgggcg caggagtttt
      421 gacggaggat ccttccaagt tcgtatccgt tatggtcctg gaagcccttc ccagtacgca
      481 ggctatttac gggttcgtta tcgcgttctt catcatcggc aggatcgatc ccgccatgac
      541 catcgcggag ggactgcacc tgtttgctgc tgctcttccg ataggcattg taggctttat
      601 ttccgccatc taccagggaa aagtcgcgtc agcaggcata cagctgatag ctaaacactc
      661 gggatcgctg gtgagtgcca tcacactggc gctgatggtc gagatgttcg ccatcctgtc
      721 tctcatcact tccatattga tgcttggctg atacggggat ccaaggcccc gacggtatca
      781 gggacctggt cggccgtacg ggggaacagg ctgatcagag cttttatgtg aaaaaggagc
      841 aatccagatg acaggaatag acaggataaa agagaaaatc cttgaggatg caaaactgaa
      901 agccagggcc acactggagc aggccgagca ggaagcccgg gacatcatgg aacaggctga
      961 gcaggaggct gcacagaaaa gggcggagct tctcgataag gcaaaagctg acggtggaca
     1021 gatgtacagg cgtatgctgg ccgtagccgg gcttgaaggc cgcaagaagc tgcttgccgc
     1081 caggcaggag atgatcgatg aggcattctc gaaagccctg gaaaggatcg agggattgcc
     1141 tgaccgccag taccaggaac tcctggaaaa aatgatctcc gaagccgcgg caggatacag
     1201 ggacccgtcg gcacagcctg acggagcgca tgccccgcag gccggcttca tagcggaaat
     1261 catgctgaat gaaagggacg ccgcgcggat ggacgggcag ttcatcgaaa atatcaacag
     1321 gaggctttcg gcactcggaa agaaagggac cgtaactctg tcggagaaga ggatcaggac
     1381 agccggagga ttcatactga aaatcgggga cgtcgagatg aacagtacgt tcgaaatact
     1441 gttcggcatg ctccgcacgg agctggaaag cgatgttgtc ggcatactgt tcggctcctg
     1501 acttctgaac ggctcaacaa ggcggcagat cgatgatgga gttattgagg gataacgatg
     1561 cttaggatca acgaaggtga ttattcatat gcttccggcc tcataagggc aaaggaaacg
     1621 aagttgctta ccgacagcca gtatgcgagg atgctggacg cctcttccgc agaagaggct
     1681 tacaaggtgc tgacggacgc aggttacggg ctcggcagtg gcagtacgca tagcgtgttc
     1741 gctttcgagc agctgctggc cgacgagatg aagaaatgct tcatgctgct ggaagagatc
     1801 gccccgaagg cagatgtgat aagaatcttt cagaaacgct atgattattt caatataaag
     1861 gtcctgctaa aggcggaact gtccgggcag gacataccgc cgatccttgc cgacacgggg
     1921 accatcggca gcgattcgat cacgcggatg atcagggaac gggattacag cggtctgact
     1981 ccgtcgatgg cggatgctgt cacgcaagtc tatgatgttt ttgccaggat gcaggatcca
     2041 caggtggtgg acctgatact ggacaaggca tcgtaccagg aattcgtcgc tgacataagg
     2101 aacatagaga gcccgttcct gcaggaactg tcggatctca tcgtcgatat gacgaatatc
     2161 aggatgttca tccgctcaag gtcgctggac aagacctggg actttatcat gaagctgctg
     2221 atagacggcg gcaccctcga cagggacgtg tattacagga acagtgacaa gccggtggac
     2281 agctttgtcg aggatatacg caaaagcagg tacggtgatg cggtgcgcaa aggccttgac
     2341 ctggtgaaat cggggaagaa cagttccggg ctcgaaaaag cgctggatga cctgctcatg
     2401 tcatacatcc gcagtgcgaa gctggtcacc ataggcgtcg agccgctcat agcgtacctg
     2461 tttgcaaagg agacagaaat caggaatgcc agaatgatca tgacgggcaa gatcaacaac
     2521 ctgccgccgg atatgatcag ggaaaggttg agggagtctt atgtataaga tcggagtgat
     2581 cggggaaaag gacgcggtgc ttggcttcat agcacttggt ttttccgttt tcccggcaga
     2641 aaacagcgaa caggctgctg aaacgctaag gaagctggct caggaccggt atgccgtgat
     2701 ctacattact gaacagacgg cggccggaat agagcaggaa atcgacaagt accgggaaag
     2761 ccgctttcct gccgtcattc ccatccccgg gatacagggg agccttggca tcggcatgaa
     2821 tggcataagg aagtgcgtag agaaagctgt cggggcggac atccttttcc gtgaatgacg
     2881 gtttgcgcag gatctgatcc ggatacaccc ggaacgaggg atcattcagc aggcggatcg
     2941 gcctgcttca gtcataagga ctgcatagat gtacaggata ctatctgaaa gcgagggatt
     3001 ggtttgagcc aggggaggat aataaaggtc tcgggccctc ttgtaatagc tgaggggatg
     3061 cgggacgcca acatgttcga cgtggtccgc gtcggcgaaa aacagctgat cggagagata
     3121 cttgagatcc acggtgacag ggcgtccatc caggtgtacg aggagacgtc cggcctgggg
     3181 ccgggaagtc ctgcggtatc caccggagag ccgctcagcg tggaattggg tccgggactg
     3241 ctggagaata tatacgacgg tatacagcgt cctttggaag tgatgcggca gacagtcgga
     3301 agcaatatca cccgcggtat aaatatcccg gctcttgaca ggagcaggaa atggccgttc
     3361 aaacctgccg tttccagggg cgacatggta gtcccgggag atatactcgg aacggtccag
     3421 gaaaatgtga ttttcgagca caggatactg gtccctgtcg gagtcgaggg cgtggtcgaa
     3481 gacatcagcg agggcgaatt tactgtcgag gaaacggtgg ctgtcataaa aactccggac
     3541 gggacatcca ggaacctgac gatgatgcag aaatggccgg tcaggaaaga gagaccgtac
     3601 aagcagaagc ttgcgccgga tgaaccgctg gtaacagggc
//
LOCUS       DAOSCC010000232         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_16538_length_3585_cov_3.967705, whole genome shotgun sequence.
ACCESSION   DAOSCC010000232 DAOSCC010000000
VERSION     DAOSCC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16538_length_3585_cov_3.967705"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLG72_10305"
     CDS             <1..1450
                     /locus_tag="PLG72_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOJ81229.1"
                     /translation="GWFYTLLAISTLLFDKPSFKNVIVLGLVNDKDGQKMSKHKGNVI
                     DPWTVLDKQGADAVRWYFYTGSAPWLPSRFYEEAVSESQRKFMGTLWNTYAFYVLYAN
                     IDKFDPTKYKLEHDKLSVMDKWILSRLYTLIGNVDKNLENYRVTESARDIQDFTDELS
                     NWYVRRCRERFWQKEMNQDKINAYMTLYTVLVELSKTIAPFVPFISEQIYLNLVRSVD
                     KSAPESVHLCDFPTVREGFIDKELEENMDKVLKYVVLGRACRNTANIKNRQPIGTMFI
                     KDSEELPGMYADLVRDELNVKQIVFTSDASGFTTYKFKPQLRTVGPKYGKLAPKIGEY
                     LSTVDGNELMDRFNRDGKVTFSLEGSDVTLELSDVLVETAQKEGFVSESERDITVVLD
                     TNLTEELIEEGYVREIISKLQTMRKEAGFEVQDRITVYYGDNEKISGIVERNSELISE
                     EVLADRLLPSTGEGYSKEWNINGENVRLTVKR"
     gene            1560..2669
                     /gene="aroB"
                     /locus_tag="PLG72_10310"
     CDS             1560..2669
                     /gene="aroB"
                     /locus_tag="PLG72_10310"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOJ81230.1"
                     /translation="MERITVELGERSYPIYIAADFSVLGNAFRKAGIGGKVLVVTDSN
                     VGRLYLETCMAELASAGALVCSHVIEAGEKSKNLDTVKDIYRQLAAQRFDRNSTIAAL
                     GGGVTGDIAGFAAATYMRGISYVQIPTSLLAQADSSIGGKTGVDFEGVKNIVGAFYQP
                     RFVYINIGTLRTLPLRELRSGLAEVIKHGLIKDAGFYDYIRLNMRKIFEFDESTLQHI
                     TRANCSIKKSVVEQDEKESGLRAVLNLGHTIGHAVESVSGFSLLHGECVSIGIAGAFR
                     LALKMGMVSNTVAEEVELTLEAAGLPVRAAGMEPKQIHAEMLHDKKAKGGKLNFVLPT
                     RIGEVTVRTLDDERILMEVLEELVGRDPAYKNPAK"
     gene            2815..3120
                     /locus_tag="PLG72_10315"
     CDS             2815..3120
                     /locus_tag="PLG72_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HOJ81231.1"
                     /translation="MFIRKKRFKKLTDKIDKLVMDMEKSRIREYVCYLENPKKVFFSN
                     FLAGLARGVGASIGFTLLAAVLLYVLQKIVRWNLPIIGDFINEIARIVENNLRNTRR"
     gene            3123..>3585
                     /locus_tag="PLG72_10320"
     CDS             3123..>3585
                     /locus_tag="PLG72_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOJ81232.1"
                     /translation="MNRQESEHYRQLLLKMMDETENTIELMKSHREGEQDKYSTGELS
                     NYDNHPADQGTELFYLEMNNALKVHGESIMMDIKDALDRIDRGVYGVCVHCGADIPRE
                     RLEVRPFAKMCLKCQEEYENTSVRQNMDRPNEELVIDAPFGRKYLNKQEDDE"
BASE COUNT         1041 a          784 c         1010 g          750 t
ORIGIN      
        1 gggctggttc tacacgctgc ttgccatatc gacattgctg tttgacaagc cgtcattcaa
       61 aaatgtaata gtccttgggc ttgtcaatga caaggacgga cagaagatga gcaagcacaa
      121 ggggaatgtc atcgatccgt ggaccgtgct ggacaaacag ggcgccgacg cggtacgctg
      181 gtatttctac acaggcagcg ctccatggct tcccagcagg ttctatgaag aggcagtgag
      241 cgaaagccag agaaagttca tgggaaccct gtggaatact tatgcgttct atgtcctgta
      301 tgcaaacata gacaagttcg atcctaccaa gtacaaactt gagcatgata aactgtcggt
      361 aatggataaa tggatccttt caaggctgta tacgctgatc gggaacgttg acaaaaacct
      421 ggagaattac agggtcaccg agtctgcccg cgatatacag gacttcaccg atgaactcag
      481 caactggtat gtcaggagat gcagggagcg cttctggcag aaggaaatga accaggacaa
      541 gatcaacgca tacatgacgc tttatacggt acttgtggag ctttcgaaga caatagcgcc
      601 gttcgttccg ttcataagcg agcagatata cctgaacctg gtcaggagcg tcgacaagtc
      661 cgctcctgag agcgtacacc tttgcgattt cccgacggtc cgggaaggat tcatcgacaa
      721 agaacttgag gagaacatgg ataaggtact gaaatacgtg gtgctgggac gtgcctgcag
      781 gaacactgca aacatcaaga accgccagcc cataggaacg atgttcatca aggacagcga
      841 agaactgccc ggaatgtatg cggacctggt cagggatgag ctgaacgtca aacagatagt
      901 attcaccagc gatgcttcag gcttcacgac ttataagttc aagccgcagc tcaggacagt
      961 gggcccgaag tacgggaaac tggcgccgaa aataggcgaa tatctgtcaa cggtggacgg
     1021 caatgaactg atggaccgct tcaacaggga cggaaaggtg accttcagcc tggagggcag
     1081 cgatgtcacc ctcgaacttt cggatgtgct tgtggaaact gcccagaagg aaggcttcgt
     1141 ttctgaaagc gaacgtgaca taactgtcgt gctcgatacg aacctgacgg aagaactgat
     1201 agaagaaggc tatgtccgcg agataataag caaactgcag acaatgagga aagaagccgg
     1261 gttcgaggtc caggaccgca taacggtcta ctatggggac aatgagaaaa taagcggcat
     1321 agtcgagcgg aacagcgaac ttatcagtga agaagtgctt gcagacaggc tgcttccttc
     1381 aacaggcgaa ggctactcca aggaatggaa catcaacggg gaaaatgtca ggctcacagt
     1441 aaaaagatga ccggaagaga ttacccgttt gcagaaattt atttattcag gggcttacgt
     1501 gtccgaaggc tgtatacaga gactcttcgg cggatgtgcc gtctgagcga gggaaactca
     1561 tggaaagaat aaccgttgaa ttgggcgaaa gaagctatcc aatatatata gcggctgatt
     1621 tttccgtcct tggaaatgct ttccgcaaag ccgggatagg cggcaaagta ttggtcgtaa
     1681 ctgacagcaa tgtcggcagg ctgtatctcg aaacctgcat ggctgagctt gcttccgcag
     1741 gagccctggt atgcagccat gtgatcgaag ccggggaaaa aagcaagaat cttgatacgg
     1801 tgaaagacat ttacaggcag ctggcggctc agcggttcga ccgcaattct acgatagcgg
     1861 cgcttggcgg tggcgtaacc ggtgatattg ccggttttgc cgccgccacc tacatgcggg
     1921 ggatcagtta cgtgcagata ccgacgagcc ttctggccca agcggacagc agcatcggcg
     1981 ggaagacagg ggtggacttc gaaggtgtaa aaaacattgt cggagcattc taccagcccc
     2041 gtttcgtgta tatcaatatc ggcacgctgc gcactctgcc gctgcgggaa ctcagatccg
     2101 ggctcgctga agtgatcaag cacgggctga taaaagatgc agggttttat gattacatcc
     2161 gcctgaatat gcggaaaatt ttcgaatttg acgaaagcac tcttcagcac ataacgagag
     2221 ccaactgttc catcaagaaa agcgtggtgg agcaggatga aaaggaaagc gggctcagag
     2281 ccgtcctcaa ccttggacac accatcggcc atgctgtcga gagcgtttcg ggattttcac
     2341 tgctccacgg agaatgtgtt tcaatcggga tcgcaggagc attcaggctg gcgctgaaga
     2401 tgggcatggt gagcaatacg gtggcggaag aagtggaact gactctcgaa gcagccgggc
     2461 ttccggtacg ggcagccggc atggagccga agcagatcca cgcggaaatg ctgcatgaca
     2521 aaaaagcaaa aggaggaaaa ctcaatttcg tgcttcccac acgcattggc gaagtcacgg
     2581 tccgcacgct ggacgatgag cggatcctga tggaagtgct tgaagaactg gtcgggcgag
     2641 atccggcata taaaaatccg gcaaaataaa actttgaata acaggatcca taataaaatt
     2701 ttacataata aaaatcgcct ctttagagaa tataaataag aggcgatttt gttttctgct
     2761 tgttgcatgc atttgcggag caggtggaca cctggctttg aggggtggtt ttccatgttc
     2821 atcaggaaaa agcgtttcaa aaagcttaca gacaagatag acaagctggt gatggacatg
     2881 gaaaagtcga ggatcaggga atatgtatgc taccttgaga accctaaaaa agtgtttttt
     2941 tccaactttc ttgccggact tgccaggggg gtcggcgctt ccataggctt tacgctgctt
     3001 gcggcggtcc tgctgtatgt cctgcagaag atagtacggt ggaacctgcc gataatagga
     3061 gattttatca atgagatcgc gaggatcgtc gaaaacaatc ttcgcaatac aaggaggtaa
     3121 aaatgaacag acaggaatct gagcattacc gtcagttgct gctgaagatg atggacgaaa
     3181 cggagaatac gatagaactt atgaagagcc acagggaggg ggaacaggat aaatattcga
     3241 caggtgaact gtcgaactac gacaaccacc ctgcagacca ggggacggag ttgttttatc
     3301 tggaaatgaa caatgctctc aaggtacatg gggaaagtat aatgatggat ataaaggatg
     3361 ccctggacag gatagacagg ggtgtctacg gtgtatgtgt ccactgcggc gccgacatac
     3421 cgcgagagag gcttgaagtc cgcccgtttg caaagatgtg cctgaaatgc caggaagaat
     3481 atgaaaacac atctgtacgg cagaacatgg acagacccaa tgaagagctt gtcatcgacg
     3541 cgccgttcgg cagaaaatac ctcaataagc aggaagatga cgaac
//
LOCUS       DAOSCC010000233         3563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_16694_length_3563_cov_3.883124, whole genome shotgun sequence.
ACCESSION   DAOSCC010000233 DAOSCC010000000
VERSION     DAOSCC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16694_length_3563_cov_3.883124"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2317)
                     /locus_tag="PLG72_10325"
     CDS             complement(<1..2317)
                     /locus_tag="PLG72_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81233.1"
                     /translation="MLLISMSGFTKNQNLLIAFASAAIAALVVAMAVMFINWLKMLRR
                     KEKKYTMQEDLTPKEWEQKMAELARSHVGVKCRRKNYIITWYNQAAYRKINMFREQIA
                     RTPTEIISLVPSARWLFDNFQMLYREIKKAKVTGTVYLPLPLLRQTEYAGYPRVYVVA
                     KKMIELSGGYLNRETIVRMIEAYQEENPLTEREIHALQEMLGLCLLERTIEVAEEIIR
                     ITKIKTRAEAFVRERMRENQDGQPDVSPLLVTVDSGSYRKKFRSRSDSAEIHLAAGSV
                     PFRTRADKTVFFSRADNTMRRTRADRIPFRSHDDMITFHSHVIYLLRRMSVDENEIQK
                     YISYHFEADGRTVSTADIFREEGRRQSELEAQIRTAITSLRELNQIDENELVEELSVT
                     ERILARDPAGVYTEMDSTSRAMYRTAVEKLARRTGASEPDVAEACVELAAEERKDVYC
                     SRHVGAYLIGKGYPVLRARLLNRPVRRPGDNRKLKGTLYFMLTAVLFVLVLFLLDYAV
                     RSAGGLETFKRVILALAALPLVKDAVARLVRNIMSRIVPAQQMPMMDYLEEIPDNART
                     VVVMPVIVSGMEQALDYAERLLRHYLVNRQPNLYFALLADYADAPSSRKPEDEEIRSA
                     LVSKIEQLNKMYPSANNRFMLLMRERRWSRTEDCYICWERKRGKLEEFNALLNGVDPK
                     ETSFDTLICDIELLRTCKYVITLDADSEIERDSAARLIGMIDHPLNRALVDPEKRKVV
                     EGYAIIQPIVRNHIYEKGGGSFAKIFGGVSGL"
     gene            complement(2719..3366)
                     /locus_tag="PLG72_10330"
     CDS             complement(2719..3366)
                     /locus_tag="PLG72_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ81234.1"
                     /translation="MILKAIITGILDSNCYIIEDGGEAAVIDPGTDAEDIMQVLKEHG
                     LALKYIIYTHGHFDHVCEGDALRRLTGARVVAHEDEARILEDPVLNGSALFWTARKMD
                     SPDISVKDGDVLKLGSVELGIMHTPGHTPGGICLLARDAGDRGRTGKHSTNCVFTGDT
                     LFRLSIGRTDLGAGDSRVLMASLDRLMELDDDVVVYPGHGPATTIGFERENNPWL"
     gene            complement(3394..>3563)
                     /locus_tag="PLG72_10335"
     CDS             complement(3394..>3563)
                     /locus_tag="PLG72_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81235.1"
                     /translation="GEAKIPLKAMNARTTRDQIAIIGLTLEIVDTEQLEKIIKKLRKV
                     DGVFEVTRSKG"
BASE COUNT          702 a         1035 c          875 g          951 t
ORIGIN      
        1 gcagtccgga cacgccgccg aatatcttgg caaacgagcc accgcctttt tcgtatatat
       61 ggttcctcac gatgggctgg atgatcgcat aaccctccac aaccttcctc ttctccgggt
      121 ccaccagtgc ccggttgagg ggatggtcga tcatgcctat gagcctggca gcactgtccc
      181 gttctatctc agaatccgca tccagcgtta tcacgtattt gcatgtacgc agaagctcta
      241 tgtcgcaaat cagcgtgtca aaactcgttt ccttcgggtc aacaccgttg agcagggcat
      301 tgaactcctc gagcttgccc ctttttctct cccagcagat gtagcagtct tccgtccggc
      361 tccatcgccg ctccctcatg agcagcatga acctgttgtt agccgaggga tacattttgt
      421 ttagctgctc gatttttgag acaagtgccg acctgatttc ctcatcttca ggcttcctgc
      481 tggacggagc gtctgcataa tcggccagca gcgcgaaata caggttcggc tgccggttta
      541 cgaggtaatg cctgagcagc ctttcagcgt agtccagggc ctgttccatc ccagatacta
      601 tcacaggcat cacgacaaca gtccgcgcgt tgtccggtat ctcctcaagg tagtccatca
      661 tcggcatctg ctgtgccggt acgatcctcg acattatgtt cctgacaagc cgggcaaccg
      721 catccttcac cagcggcagc gctgcaagcg caaggatcac ccgtttgaat gtctcaaggc
      781 ctccggcgct ccttacggca tagtccagca ggaagagcac gaggacgaac aagacggcag
      841 tcagcatgaa atacagcgtg cccttgagct tcctgttgtc ccctggccgg cggactggcc
      901 tgttcagaag ccttgccctg aggacaggat atcctttccc gatcaggtat gcgccgacat
      961 gccgcgagca gtatacatct ttcctttctt cagcggcgag ttccacgcag gcttccgcaa
     1021 catcgggctc gctggcgccg gtcctccggg ccagtttttc gacggcagtc ctgtacatgg
     1081 cacggctcgt gctgtccatc tcagtataca cgcctgccgg gtccctggcg agtatgcgtt
     1141 cggtcaccga caactcctcc accagttcgt tctcgtcgat ctggttgagt tccctgaggc
     1201 tggtgatagc cgtacggatc tgcgcctcga gttcggactg ccttcttccc tcttccctga
     1261 atatgtctgc tgtgctgaca gtccttccgt cggcctcgaa atggtatgag atgtatttct
     1321 gtatttcgtt ttcatcgacc gacatgcgcc tgagcaggta aataacgtgc gagtggaacg
     1381 ttatcatgtc gtcatgtgag cggaacggta tcctgtcggc gcgtgtgcgg cgcattgtat
     1441 tatcggcacg agagaagaac actgtcttgt cggcgcgtgt gcggaacggc acactgccgg
     1501 cggccagatg gatttccgca ctgtcggatc gtgagcggaa ctttttcctg tagctcccgg
     1561 agtccaccgt cacaagcagc gggctcacgt ccggctgtcc gtcctggttt tcgcgcattc
     1621 tctccctgac aaaagcttcg gccctggtct taatcttcgt aatgcggatt atttcttcgg
     1681 ctacctctat cgtgcgttca agcaggcaaa ggccgagcat ttcctggaga gcgtggatct
     1741 ccctctctgt cagcgggttt tcctcctgat acgcttcgat catgcgcact atcgtctccc
     1801 tgttcagata accgccggac agttcgatca ttttcttcgc aactacatat accctcgggt
     1861 agccggcata ctccgtttgc ctgagcagcg gcaatggcag gtacaccgtg cccgtgacct
     1921 tggccttctt tatttcgcgg taaagcatct ggaaattgtc aaacaaccac cgcgcagaag
     1981 gaacaagcga aattatctcc gtcggcgtgc gcgctatctg ttccctgaac atgttgattt
     2041 tcctgtatgc cgcctggttg taccaggtaa tgatatagtt cttcctgcgg cacttcacgc
     2101 cgacgtggct gcgcgcaagt tcggccatct tctgctccca ttctttcggg gttaggtctt
     2161 cctgcatggt atatttcttt tccttcctgc gcagcatctt cagccagttt ataaacatga
     2221 ccgccatggc cacgaccaaa gcggcaattg cagcagatgc aaatgcaatt aatagattct
     2281 ggtttttggt aaaaccggac atactgatta aaagcattcg atcactgcct ttcctttagt
     2341 tttttctttt tgtcagccgt tgtgatttac agtttcggtt tgccgtgatt cacgtgttcc
     2401 gagtcgatgt gatcaccgtc ccgttttgtg tgaccacacg tcctgatctg cgtaatcact
     2461 cgttccggtt tgcatgacca cgttgttttg cttgctgtga ttacatgtct ggtcagcttt
     2521 gcgataaaca ctacgcaaac gtgtcccgac gcaggcttga tttgcatcag ccgcgccgca
     2581 catgcaaaaa gaagttggct gaaatatgag caaataatat tattgccgtt aattgaactt
     2641 tcctgccggt gcgatcctta accgcaataa agcattatag caaagaaata tattttttgc
     2701 aaatgggcgt gcaacacttc acaaccatgg gttattttcc cgttcaaatc ctatggttgt
     2761 tgcaggaccg tggcccggat agaccacgac gtcatcgtcc agttccatca gcctgtcaag
     2821 cgaggccata agcacacggg aatcgcccgc tcccaggtcc gtcctgccga tgctgagcct
     2881 gaagagagtg tcgccggtga agacgcagtt cgttgaatgc ttgccggtgc ggcctctgtc
     2941 accggcgtcc ctggccagca gacagatccc gcccggcgtg tggccaggcg tatgcattat
     3001 tccaagctcg acgctgccga gcttcagcac atctccatcc ttcacggaaa tgtcgggact
     3061 gtccattttc cttgcagtcc agaatagtgc cgatccgttc agcaccggat cttcaagtat
     3121 ccgcgcctca tcctcgtggg cgaccaccct ggcgccggtc agtctgcgca gagcgtcgcc
     3181 ttcgcagaca tgatcgaaat ggccatgggt atagatgata tatttcaaag ccaggccatg
     3241 ctctttaagt acctgcatga tatcttcggc atccgttcca ggatctataa cggcagcctc
     3301 gccgccgtct tctatgatat agcagtttga atcgagtatg cctgtgatta ttgctttcag
     3361 gatcatttcc gcataactcc cgtcctaata ttgtcagccc ttgctcctcg tgacttcaaa
     3421 tacgccgtcc accttgcgga gcttttttat tatcttctcc aactgttcgg tgtctactat
     3481 ctcgagcgtc agcccgatga tggctatctg gtcgcgcgtc gtccttgcgt tcatcgcctt
     3541 gagcggtatc ttcgcctcgc cca
//
LOCUS       DAOSCC010000234         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_17179_length_3492_cov_3.702939, whole genome shotgun sequence.
ACCESSION   DAOSCC010000234 DAOSCC010000000
VERSION     DAOSCC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17179_length_3492_cov_3.702939"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1625
                     /locus_tag="PLG72_10340"
     CDS             345..1625
                     /locus_tag="PLG72_10340"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOJ81236.1"
                     /translation="MVKKEIIALLLAGGQGSRLGVLTKKSAKPAVLFGGKYRIIDFSL
                     SNCANSGIDTVGVLTQYQPLKLNNHIGIGKPWDMDRMHGGVTILSPFMKEDSGEWYKG
                     TANAISQNIDFVDKQAPKYVIILSGDHIYKMNYSDMLAFHRKNNAEATISVINVSYEE
                     ASRYGIMSTDEQGRIYEFQEKPENPKSTLASMGVYIFTWEILREYLIRDDQREDSDHD
                     FGKDIIPAMLADGRRMFAYRFNGYWKDVGTIQAYWEANMDLVKRVPEFNLYDPEWKIY
                     TPNPVKPPHYVAPGGSVKTSVVAEGCMIYGNVRNSVLFSGVFVEKDALIENSIVMSNS
                     HIGEGSVLDHCIIGENVRVGKNVRIGFGDDVPNTDKPGIYSSGITVVGEGAEIPDETV
                     MGKNVMIDMYAGAEDFCCKTVPSGASVYKGGECK"
     gene            1631..2752
                     /gene="glgD"
                     /locus_tag="PLG72_10345"
     CDS             1631..2752
                     /gene="glgD"
                     /locus_tag="PLG72_10345"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOJ81237.1"
                     /translation="MTNTMGLILSGWKKPALKDLSYKRSVSAIPFGGKYRAIDFILSN
                     MVNSGIKNVGVLTQYSFRSLMDHLGSGKEWDLDRRYDGLFIFPPTVSDEESGWYKGSA
                     DAMYHNLSFLKRSNEEYVVIAQGNGIFKMQMGDMLQYHIDKNADITLAYRRMYDYTAE
                     ELSGLGIIRIDEDSRITDLQEKPLNPQSDLASIGVYMLRRTLLISLIEECIAHGNYDF
                     VKDIMIRKLDRLNIYGYEFRGYWRSISSVRNYYRCSMEVLDPEIREELFIRNGKIYTK
                     VKDEAPAKYNEEAEVTNSIVADGCFIEGTVENSVLFRGVTVAKGAVVRNSIIMQGSVI
                     EPDAVVEYAILDRNVVLTRGKYLKGDTDYPVVVSKNSRV"
     gene            3191..>3492
                     /locus_tag="PLG72_10350"
     CDS             3191..>3492
                     /locus_tag="PLG72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ81238.1"
                     /translation="MTDKLKADLSLLIITVVWGASFPLIKVVLGYIPAFAYLSLRFLL
                     AAAVLAAIYHRNLKKINKRALKYGCILGLFMFGGMAFQVVGLYTTSASNSGFITGL"
BASE COUNT          981 a          774 c          968 g          769 t
ORIGIN      
        1 caaggtgtct cctttaattt tacattatat gttagcgctt ttgctcattt atagatatac
       61 atgcaatttg gataaacaac acgtgattta tgtaccgcgg acaggcaaca aacgaaaata
      121 cccggcgggc tttgtaactg gagcattcag cagtctttat aatttcaggc gcctgtgtta
      181 tcattactat taagggaaaa aatgtgacaa ctgtcgactt gcgtttgtat cgatgcggtc
      241 atgtgcttcg gcttatctgg tgtgccacag gaaatgatac atatttattg aaatctttca
      301 tatagattga tagaacgtca gataaaacac gggggtggtg tgagatggta aagaaagaaa
      361 tcatagccct gctgctggcc ggaggacagg gaagcaggct gggggtcctg accaagaaat
      421 ctgccaagcc tgcggtgctt ttcggcggga aatacaggat aatcgatttt tcgcttagca
      481 actgcgccaa ttcaggcatc gatactgtcg gggtgctgac gcagtatcag ccgctcaagc
      541 tgaacaatca catcggcatc ggcaagccgt gggacatgga caggatgcac ggaggcgtca
      601 cgatactgtc gccgttcatg aaggaagaca gcggcgagtg gtacaaggga actgccaatg
      661 ccataagcca gaatatcgac tttgtcgaca agcaggcgcc caaatacgtg ataatccttt
      721 ctggcgacca tatctacaag atgaactact ccgacatgct cgctttccac cggaagaaca
      781 acgcggaagc gacgatatcc gttatcaacg tttcatacga ggaagcgtcg aggtacggca
      841 ttatgagcac ggatgaacag ggacgcatat acgagttcca ggagaagccc gagaacccga
      901 agagcacgct ggcttccatg ggcgtttata ttttcacctg ggagatactg agggagtacc
      961 tgatacgcga cgaccaaagg gaagactccg accacgattt cggaaaggac ataatacctg
     1021 caatgcttgc cgacggaaga aggatgtttg cttacaggtt caacggttac tggaaggatg
     1081 tagggacgat acaggcctac tgggaggcga acatggatct tgtcaaaagg gtcccggaat
     1141 tcaacctgta cgatcccgaa tggaagatct acacgcccaa tcccgtcaaa cccccgcact
     1201 atgtcgcgcc gggcggaagc gtcaaaacat ccgtggtggc cgagggatgc atgatatacg
     1261 ggaatgtcag gaattcggtg ctattctccg gtgtttttgt cgagaaagac gcgcttatcg
     1321 aaaattccat cgtgatgtcc aattcgcata taggagaagg aagcgtcctg gaccactgca
     1381 tcataggtga aaacgtcagg gtcgggaaaa atgtgaggat aggctttggc gacgatgttc
     1441 ccaacacgga taagccgggc atatacagtt caggcataac ggtagtggga gaaggggctg
     1501 agatacctga cgaaactgtc atgggcaaga acgtgatgat agatatgtac gccggcgctg
     1561 aggacttctg ctgcaaaacc gtgccgtcgg gcgcaagcgt atacaaaggc ggtgaatgta
     1621 aatgacgatg atgacgaata ccatgggact gatactgtcc ggctggaaaa aacctgccct
     1681 caaggacctt tcatataagc ggtctgtgtc cgcgataccg ttcggcggca agtacagggc
     1741 aatagatttc atactgtcca acatggtcaa ttccggcatc aagaatgtcg gggtgctgac
     1801 ccagtacagt ttccgttcgc tgatggacca tcttggctca ggcaaggaat gggaccttga
     1861 caggagatat gacggcctgt tcattttccc tccgaccgtg tcggacgagg aaagcggctg
     1921 gtataaaggc agcgcggatg cgatgtacca caacctgtct ttcctcaaac ggagcaacga
     1981 ggaatacgtg gtcattgccc agggaaacgg cattttcaag atgcagatgg gcgacatgct
     2041 gcagtaccac atagacaaaa atgccgacat aacgctggca tacaggagaa tgtatgacta
     2101 taccgctgag gagctttcgg gtcttggtat aatcaggatc gacgaggaca gcaggataac
     2161 cgacctccag gagaaaccgc tgaatccgca gtcggacctg gcttccatcg gcgtttacat
     2221 gctgaggagg acgctgctca tctcgctgat cgaggagtgc attgcccacg gcaattatga
     2281 tttcgtcaag gacataatga taaggaagct cgacaggctg aatatttatg gttacgaatt
     2341 caggggctac tggcgaagca tcagttccgt ccgcaattac tacagatgct ccatggaggt
     2401 gctcgatccc gaaatacgcg aggagctttt cataaggaac ggaaagatat acaccaaagt
     2461 caaggacgag gctcccgcaa aatacaacga ggaagcggaa gtaaccaatt ccatcgttgc
     2521 agacggctgt ttcatcgagg gcaccgtgga gaacagcgtg ctgttcaggg gagttaccgt
     2581 agccaagggc gccgtggtga ggaacagcat aataatgcag ggttcggtga tcgagccgga
     2641 tgcggtcgtg gaatacgcca tacttgatag aaatgtagtc ctgacaaggg gcaagtacct
     2701 gaaaggcgat accgactacc ccgtggtcgt gagcaagaat tccagggtat aatggcttag
     2761 gaatacggaa gctaatcagt tccgggcata aatcaccgga gcatataacg ctccaggctg
     2821 taaaaaccag tgaacgggac atttaggaca ggaatcggaa aacgattacg gggcatatag
     2881 ctgaaagctc gaaaacagaa ggccggaaca caaagtgtaa caaaagaaaa gaaacagaat
     2941 agccggtggc cggagcataa ggcctgtggc ctgaccggcc atattgatgt atgattcagt
     3001 tttagtgtat aatcaggtga gtgccggcag gcactgagac aggcaatgga gtccatgcat
     3061 ttacagggcg ctttgtcagc cttgtactca gaacgggatt ttactgaaat acagacggga
     3121 ttttactgaa atatcgatac gaaaggacaa aagcttttac agtacgaaag cttgaaatgg
     3181 gttgacatat atgactgaca aactcaaggc ggatttgtcg ctgctgatca taacagtggt
     3241 atggggcgca tcgttcccgc tgatcaaggt ggtgctgggg tacatcccgg catttgctta
     3301 cctctcactg aggtttttgc tggcagcggc agttttggca gcgatctacc acaggaattt
     3361 gaaaaaaatc aataaaaggg cgctgaaata cggatgtatc ctcggactct ttatgttcgg
     3421 cggtatggca ttccaggtcg tcggcctgta taccacgtcc gcgtcgaatt ccgggttcat
     3481 caccggcctg aa
//
LOCUS       DAOSCC010000235         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_17244_length_3481_cov_4.864857, whole genome shotgun sequence.
ACCESSION   DAOSCC010000235 DAOSCC010000000
VERSION     DAOSCC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17244_length_3481_cov_4.864857"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLG72_10355"
     CDS             <1..766
                     /locus_tag="PLG72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ81239.1"
                     /translation="ANAVKLAAAVEVLHLATLVHDDVIDDADIRRGLPTLQKKFGKRT
                     AVICGDYLLSRALQIAASIENKKDYLDIDVPDYVARICAGELSEQINAGNYGLSIYQY
                     LKIIAGKTAALFEASFYAGAVLSGCDRSEYRAYKKLGYYLGMIFQLTDDCMDFEATES
                     VAQKPVQSDFEKGVVTLPVIRALQTDAGLKEKAARKEVSADEIGRAVVRAGGLDFTKL
                     VAKRYHSKYMKLLDGLDAPDEKKRRLAELVDRALNR"
     gene            1316..2215
                     /locus_tag="PLG72_10360"
     CDS             1316..2215
                     /locus_tag="PLG72_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOJ81240.1"
                     /translation="MIKRFLSYVEIKTKITSTFAFMLTITFLLYSGRPIDWLRTLVFF
                     LAMFLFDLTTTAINNYIDTKTNEQVLQFERRTALTIILILFTISTALGLYLAYLTDVV
                     VLLLGGLCFLTGVFYTYGPVPISRMPLGEVMSGLFYGLFIPFLLLYINMPEGTYLTLG
                     LGWESIRLELNVMPLISVLLLAVTPMCTTANIMLANNTCDVEKDVKVRRYTLPYYLGK
                     KALWLFALLYYACYASIVAMVVLRILSPVCLVSLLTIIPVQRNINIFMERQDKASTFI
                     TSIKNYIIIMGSLTITVFVSAAL"
     gene            2424..2828
                     /locus_tag="PLG72_10365"
     CDS             2424..2828
                     /locus_tag="PLG72_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOJ81241.1"
                     /translation="MKLFKKTDILIIAVIIAAAAAIWYFYSNAVSGRPVKAEIYYYSD
                     LVGTVELEPGPERIFSIPQDEDVVLMIDTDGSIRFIESDCPDKICIRTGRLRLAGQSA
                     ACLPNGVIVKIVPADGWKESDPDIVIGSRGGQ"
     gene            2825..3445
                     /locus_tag="PLG72_10370"
     CDS             2825..3445
                     /locus_tag="PLG72_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOJ81242.1"
                     /translation="MSKANGGPLAWNGSDRVLAEKKSNGIFNDPASVDGARNESRRTR
                     KLVLSGMIFALALVLSIVENAFPPLPLPVPGVKFGLSNIAVMYALFFLSRGQAYSIAV
                     LKSLFVFMTRGLIAGILSLSGGILSLTVMLLVMVLFRDKASYTAISISGAIAHNTGQF
                     IAVSFIYTGMYLWAYLPVLLVSGVIAGIVTAVLLRFIIPAFERLRL"
BASE COUNT          923 a          766 c          959 g          833 t
ORIGIN      
        1 cgcaaatgcg gtaaaactgg cggcagcagt agaggttctg cacctggcga cacttgtcca
       61 tgatgacgtt atcgatgatg cggatatcag gcgtggactt ccgacactgc agaaaaaatt
      121 cggaaagagg accgcggtga tatgcggcga ttaccttctg agcagggctt tacagatcgc
      181 ggcgtcgatc gaaaataaaa aagattatct cgatatcgat gtaccggact atgtggcaag
      241 gatatgcgca ggcgaactga gtgagcagat aaatgccggg aattacggcc tttcgatata
      301 tcagtatctc aaaataatcg cagggaagac agcggcgctg ttcgaggcat cgttttatgc
      361 aggtgccgtc ctttccggct gcgatcgttc cgagtacagg gcatacaaaa aactggggta
      421 ctatctgggc atgattttcc aactgacgga tgactgtatg gattttgagg cgacggaaag
      481 cgttgcgcaa aagcccgtgc agtccgattt cgaaaaaggc gtggtgactc tgcccgttat
      541 ccgtgccctg cagacggatg cgggccttaa ggagaaagcc gccaggaaag aggtgtctgc
      601 agacgagatc ggccgggctg ttgtcagggc cggaggcctg gactttacaa agctggtggc
      661 aaaaaggtac cacagtaaat atatgaagct gctggacggg ctcgatgcgc cggacgagaa
      721 aaagagaagg ttggcagagc ttgtagaccg ggcgttgaac aggtagggcc gcagttccgt
      781 ggatacggtt ttgcacggca gctacaggtt ctgtacagtt gatgcatgta taatcagcaa
      841 aatggtaagc tgagtgtaca aaattcgaat tgttgcggct aaaaacagtg aaaacattgt
      901 aacttgtcaa taaacgggtt acataatgta aagttccatt gcattatggg gcttgatacc
      961 taaatgattg agcaaacatt catacaacaa tcaaaatttt gtacaccctt gtccttattt
     1021 cccggggaaa taaatgcatg gacaggaaac catgcatacc gtaaatggag acactagatg
     1081 cctgaggaca gtgattactg aaacgggaac cgctactgac agtgataatt gagacggaaa
     1141 ctgctactga ctgtgattac tgaaacggaa accgcaacgg aaagaaaagc ataccggaaa
     1201 caacggaaag aaacgtatac cggaaagaaa gccggggagt tcataagtca gagcataatt
     1261 cagagaatta taggatttca gaaacgaatc tcaggagaga ggtgcatgag aagttttgat
     1321 taagcggttt ttgagctatg ttgagatcaa aacgaaaata acaagcacat ttgccttcat
     1381 gctgaccatt acgttcctgc tgtacagcgg ccggccgatc gactggctga ggacgctggt
     1441 attcttcctc gccatgtttt tgttcgattt gaccactaca gccatcaaca actatattga
     1501 tacgaagacc aacgaacagg tgctccagtt cgaaaggcgc accgccctga ctattatcct
     1561 gatactgttt acaatcagca ccgcgctggg tttgtacctg gcatacctga cagacgtcgt
     1621 ggtgctgctt ctgggcggat tgtgcttcct gacaggcgtg ttttatacat acggcccagt
     1681 gccgatatca aggatgccgc tgggtgaagt gatgtcggga ctgttttacg ggttgttcat
     1741 accattcctg ctactttata tcaacatgcc ggaggggaca taccttacgc tcgggctggg
     1801 atgggagagc atcaggctcg aacttaatgt catgcccctg atatcggtcc tgctgcttgc
     1861 ggtgactccg atgtgtacta cggccaatat aatgcttgcg aacaatacat gtgatgtcga
     1921 aaaggacgtg aaggtcagaa ggtacacatt gccctattat cttggcaaaa aggccctgtg
     1981 gctgtttgcg ctcctgtatt atgcgtgtta tgcctctatc gtggccatgg tcgtgctcag
     2041 gatactttcg cctgtttgcc ttgtttcgct gctgaccatc attcctgtcc agcggaacat
     2101 caatatattc atggaacgcc aggataaagc ctctactttt ataacctcca tcaaaaacta
     2161 cataataata atgggatcgc tgaccatcac ggtgtttgtc agcgctgcgc tgtaaccggc
     2221 aggatggctt gcgctgcagt cgatgggcag aacatcggga taacaggcgg atacctgtat
     2281 tacaaacaga cactttaatt atggacagaa ccgtcatatc agcaggcccg gcatcaactg
     2341 actgcacaaa aaatgggaaa tctgcgtgtt tatgcgtcat gtctgagggt atatattatc
     2401 aagcacggtg gaagggtaaa gccatgaagc tgttcaagaa aaccgacatc ctgataattg
     2461 cggtgatcat cgctgcggca gcggcgatat ggtattttta cagcaatgcg gtttcaggca
     2521 gaccggtcaa agcggagata tactactact cggacctggt cggaacggtt gagcttgagc
     2581 ccggaccgga aaggatattt tcgatcccgc aggacgagga tgtggttttg atgatcgata
     2641 cggacgggag catcaggttc atcgagtccg attgcccgga caagatctgc atcaggacag
     2701 gcaggttgcg tctggcaggc cagagtgccg cttgcctgcc caatggcgtg atcgtgaaga
     2761 tcgtaccggc ggacggatgg aaggaaagcg atcctgatat agtgatcgga agcaggggag
     2821 gacaatgagc aaagcaaacg gcggaccatt ggcatggaac ggaagtgatc gcgttttggc
     2881 agaaaagaaa agcaacggca tatttaacga tcccgcatcc gtggacggcg ccaggaatga
     2941 gtcgcgaagg acaaggaagc tggtgctttc ggggatgata ttcgcgcttg ccctggtcct
     3001 gtcgatcgtt gagaacgcgt tcccgccgct gccgctgccg gtgcccggcg tcaaattcgg
     3061 actgtcgaac attgcagtaa tgtatgcgct gtttttcctt tcgagaggcc aggcatactc
     3121 catagctgtc ctgaagagcc tgttcgtttt catgacgcga ggcctgatcg caggaatact
     3181 gagcctttcg ggtggaatac tatccttgac ggtcatgctg ctggtaatgg tactgttcag
     3241 ggacaaagca tcatacacgg ccataagtat atcaggagcc atagctcata atacgggaca
     3301 gttcattgcg gtttcattta tttacaccgg tatgtattta tgggcatatc tgccggtgct
     3361 gctcgtgtcc ggcgttatcg cgggcatcgt cacagccgtg ctgctgaggt ttattatacc
     3421 ggccttcgaa aggctgcgtt tataacattg cacagaattt gacggaggaa aacaagtagt
     3481 a
//
LOCUS       DAOSCC010000236         3441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_17489_length_3441_cov_4.551388, whole genome shotgun sequence.
ACCESSION   DAOSCC010000236 DAOSCC010000000
VERSION     DAOSCC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17489_length_3441_cov_4.551388"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1342
                     /locus_tag="PLG72_10375"
     CDS             413..1342
                     /locus_tag="PLG72_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOJ81243.1"
                     /translation="MIEIFKTVNNELVTTDTFEEGVWVDMVNPSEEEINRVSSSLNVE
                     IDFLRAALDEEERARIESDEGQTLIVADTPIVENEGQMNIYYTIPLAIIILKHAIITV
                     CLNEDTILEDFRKNRVKSFLTQYKTRFVLQILYRNAVRYLLYLKQIDKMSNKIEQDLH
                     KSMRNKELIQMLKLEKSLVYFSTSLKANEVVLEKLLRLENIKKYPDDTDLLEDVIVEN
                     KQAIEMANIYSSILTGTMDAFASVISNNLNIVMKFLTSVTIVMAIPTLISSFFGMNVP
                     LPLNSPGSFWIIVAITLAVCTVTGFTLYKKKLF"
     gene            complement(1473..1706)
                     /locus_tag="PLG72_10380"
     CDS             complement(1473..1706)
                     /locus_tag="PLG72_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81244.1"
                     /translation="MVLLYSLKEQFYDGQGVLRNPGERYQDREGIFREPGEDFVDYMG
                     MLRRADEEFYDSQSILRQPGESFYDGAGYLRER"
     gene            complement(1773..2453)
                     /locus_tag="PLG72_10385"
     CDS             complement(1773..2453)
                     /locus_tag="PLG72_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOJ81245.1"
                     /translation="MGLRKKLGIKKVLIFILLTGCIGLAALFGINEYVKASVRDRIIT
                     AEEAAGLDADCILVLGAHVAGETPSLMLRDRLDCGIELYKAGASDRLLMSGDHGRKNY
                     DEVNVMKQFAIDAGVPSSHVFMDHAGFSTYESLYRARDVFISKKIIIVTQEYHLYRAL
                     YIAERLGLEAYGVAAAHAEYPGQSYREFREVLARTKDFFYVIAKPEPTYLGEAIPVDG
                     DGDVTNDK"
     gene            complement(2849..3379)
                     /locus_tag="PLG72_10390"
     CDS             complement(2849..3379)
                     /locus_tag="PLG72_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ81246.1"
                     /translation="MYESITYAELKSLPRDQKIEALKELMATCSSQKKIAEKLGVNPP
                     NIYSMISRYVKEDTDKKPRKRKNRMKSKEEIANKVTGDQAARNEQIVCGDAANELFEA
                     NTGENVQVAETAPARQTREGADTDAQAEWPGQNDFLVSIEKELTGEELGPMLAAIGNV
                     LLKNTKYRICMKITEK"
BASE COUNT          878 a          866 c          747 g          950 t
ORIGIN      
        1 aactgggttt agcctcataa acaatttttg gatttatttt tgctcattta tagtacatgg
       61 agaaaaaaga tcaaataatg caaataaatc gtcgatatgt acacgtcgaa ttattccgga
      121 acagcaatac gctcagtccg ccattgatct tgccgatttt gcttgaaaac ctgccggcca
      181 gggggtataa tttaaaaggt ccttgaaaaa ggcactcaaa tcctgtcttt tattactgct
      241 taacacggag gtgaacagta tggaatgtga cactggtggc ggtagtagtc acttgtggcg
      301 cctcatcaaa atgagggttg gtctgccatg ctgtttgccg gccgtaaaaa taccgtaaac
      361 atgcatggat agatcaccct ttacaaaaaa tcaaatagaa agggtgattt cattgatcga
      421 gatcttcaag acagtgaaca acgaacttgt caccaccgac actttcgagg aaggcgtctg
      481 ggtcgacatg gttaacccgt ccgaagaaga gatcaacagg gtcagcagtt ctctgaacgt
      541 tgaaatagac tttttgaggg ctgcactgga cgaagaagaa agagccagga tcgagagcga
      601 cgagggacag acgctgatcg ttgccgatac tccgatcgtc gaaaacgagg gccagatgaa
      661 tatatattac acgatacctc tggctatcat aatcctcaaa catgcgataa taacggtatg
      721 cctcaacgaa gacaccatac tcgaagattt caggaaaaac agggtcaaat ctttcctgac
      781 gcagtataaa acaaggttcg tcctgcagat actgtaccgg aatgcggtaa gatacctgct
      841 gtacctgaag cagatcgaca agatgagcaa caagatagag caggacctgc acaaatccat
      901 gaggaacaaa gagctcatcc agatgctgaa gctggagaag agccttgtat acttctcgac
      961 ttcgctgaaa gcgaatgaag tggtgcttga aaaactgctg aggcttgaaa acatcaagaa
     1021 atacccggat gacaccgatc tgctggagga cgtgatcgtc gagaacaaac aggcaatcga
     1081 gatggccaat atttacagca gcattctcac cgggaccatg gatgcgttcg catcggtcat
     1141 ttcaaacaac ctgaatatag tgatgaagtt cttgacatcg gtaacgatag tcatggctat
     1201 ccctacactg atatcgagct tcttcggcat gaacgtgccg ctcccgctga acagcccggg
     1261 ttcgttctgg atcatcgtgg ccataaccct ggcagtgtgt acggtcacag ggttcacact
     1321 gtacaagaaa aagctcttct gaagatgttg ggatggaaag aaaagcggtc agcgttctgg
     1381 ctcccgggtc agaacgcttt tctcatattg tgcggctgct tgcccggctg atcactgttc
     1441 cgccagtact gtgaggctgc tgccgcggcc ggtcaccgtt cccgcaggta ccccgctccg
     1501 tcatagaagc tttcgcccgg ctgcctgagg atgctctggc tgtcatagaa ttcttcgtcc
     1561 gctctcctca gcatacccat gtaatcgacg aagtcttctc ccggttcccg gaagatccct
     1621 tccctgtcct ggtacctttc ccccgggttc cggaggaccc cctgcccgtc atagaattgt
     1681 tccttcagtg aatacaacaa aaccacctta taaagatctt ctatatgatt atatcataga
     1741 ctgttcatga cgtatatatg aatgacggac attcatttgt cattcgtaac gtccccgtcc
     1801 ccgtcgacag gtatcgcttc gccgagatat gtcggttccg gcttggcgat cacgtaaaag
     1861 aagtcctttg tcctggcaag tacttccctg aattcacggt aagactgccc cggatattcg
     1921 gcatgggccg ctgcaacgcc gtaagcttcg aggccaagcc tctccgcgat gtacagtgcc
     1981 ctgtaaaggt ggtactcctg ggtcacgatg attatctttt tcgaaatgaa aacatccctt
     2041 gcgcggtaca ggctttcata ggtggaaaac cccgcatgat ccatgaagac atgcgaagag
     2101 ggaacgcctg catctatcgc gaactgtttc ataacattga cttcatcata attcttcctg
     2161 ccgtggtctc cgctcatcag aagcctgtcg gatgccccgg ctttatacag ctcgatgccg
     2221 cagtcgagac ggtccctcag catcaggctc ggtgtttccc ctgcaacgtg cgcccccagt
     2281 acaagtatgc agtccgcatc aagacctgcc gcttcctccg cggtgatgat cctgtccctt
     2341 accgaagcct ttacatattc atttattccg aataatgcgg ctaaaccaat acaccctgtg
     2401 agtaagataa aaatcaatac ttttttgatc cccaatttct ttcgtaaacc catagccagg
     2461 tattctcctg ttattatgcg acgatgtaaa atttatatta taaactgata atttataatt
     2521 gatattcaaa accgttattt acatgctgaa aacagttaac tgcccgatat ttccgttcga
     2581 tatattcaca tgatccgacc gaaatttcta tatgatattt taccggtatg cctcccgcat
     2641 tttcgttaaa tacactgcag tcatttgttt aaaatgtact acgcaacatt ttagacatgc
     2701 tccggctgcc aaatgttcct tttcctgtac gctccaatct gcgaaacatg ccgggttaat
     2761 ccgcagaata cctgcggaca gcaaaaaagg ccttgtaggt tgcaaaggcc ttgtaatttg
     2821 ccgaatttgt gatgccatca cgaattggct atttctcagt tatcttcatg caaatcctgt
     2881 attttgtgtt tttcagcaga acgttcccga ttgcggccag catgggccct aactcttcac
     2941 ccgtcagttc cttttctatt gagacgagga aatcattttg gccgggccat tccgcctgcg
     3001 cgtctgtgtc agccccttcc ctggtctgtc gtgccggggc cgtttctgcg acctgaacat
     3061 tctctccggt atttgcttcg aacagttcat tcgcagcatc tccgcaaacg atttgctcat
     3121 tcctggctgc ctgatccccg gttactttat tagcaatctc ttctttactt ttcatcctgt
     3181 tctttctctt cctcggcttt ttatccgtat cttccttgac gtaccttgat atcatactgt
     3241 aaatatttgg cggattcacg cccagctttt ccgctatctt cttctgagac gaacaggtag
     3301 ccatcagttc cttcagcgct tcgattttct ggtctcttgg caggcttttg agttcagcat
     3361 atgttatact ttcatacata cgttttcaca tccttgtaca attaatattg tattaaatca
     3421 gtgaccgaag ccactgttcg a
//
LOCUS       DAOSCC010000237         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_17554_length_3432_cov_4.799822, whole genome shotgun sequence.
ACCESSION   DAOSCC010000237 DAOSCC010000000
VERSION     DAOSCC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17554_length_3432_cov_4.799822"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1037)
                     /gene="recO"
                     /locus_tag="PLG72_10395"
     CDS             complement(285..1037)
                     /gene="recO"
                     /locus_tag="PLG72_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ81247.1"
                     /translation="MEMSFLKTEGIVVKEVSTGEADKIITILTRGQGRISALVKGGRR
                     PRSRLSAGSQVFCYGDFVLFKGRDIYSVNSCEVIEPFYEIRNDVIKLTYAAHFTDIVL
                     EMIQENQPSPKLLKLFLNSLHMLAKTSKEPDLITSIFELRAVSISGFAPHVQACMVCG
                     DAPEKSYSFSFRDCGFLCDRPGCAEHDGSAVPLSVGASRAIQHIVYAPMKELFSFGLS
                     GEVLDEISRITRRYLRERLERDFTKLDFLKNI"
     gene            complement(1065..1220)
                     /locus_tag="PLG72_10400"
     CDS             complement(1065..1220)
                     /locus_tag="PLG72_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HOJ81248.1"
                     /translation="MLKDFAWNTFVSKGILESYIFYREIKEKERERAAEQRRLAEAEA
                     ATSTSEA"
     gene            complement(1383..2270)
                     /gene="era"
                     /locus_tag="PLG72_10405"
     CDS             complement(1383..2270)
                     /gene="era"
                     /locus_tag="PLG72_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ81249.1"
                     /translation="MFKSGFVSIIGRPNVGKSTLTNRLIGEKLSIISSKPQTTRNTIK
                     AIINTSDSQIIVIDTPGIHKPKTKLGEYMVKLAESTLNEVDLILFLAEATDKIPGAGD
                     LHIAEQLKTVKTPVILIINKIDLVKKEELLETIRNFSQLMDFSAVIPISAASGEGTDI
                     IIDEIKKLLPEGPKYFPDDVITDQPERTLAAELIREKILELVSDEVPHGTSVEIVSFR
                     EREGKGIIDIEANIYCERESHKGIIIGKQGAMLKKIGTLARVEMENLLGAKVFLKLWV
                     KVKPDWRNSSSMLRTLGYQ"
     gene            complement(2301..2999)
                     /locus_tag="PLG72_10410"
     CDS             complement(2301..2999)
                     /locus_tag="PLG72_10410"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOJ81250.1"
                     /translation="MKNKSLIDSFNNAINGIFSAVKSERNMKIHIIAAALVLGLSLFY
                     DLTRIEFIIVCMTIALVIICELFNTAIEVLIDTLIGIYHPRAKTVKDTAAGAVLVSAL
                     LSLVVAYFIFFDRVSTSLESGISRIKQAPMHLTVIALVLTMAAVLVLKLVFKRGTPFS
                     GGMPSGHSAIAFSAATAVALYSNNAGITILFIIVALLVVQSRLEGKIHNILELMAGAV
                     IGFLVTLLLFQIFS"
     gene            complement(2996..3424)
                     /gene="ybeY"
                     /locus_tag="PLG72_10415"
     CDS             complement(2996..3424)
                     /gene="ybeY"
                     /locus_tag="PLG72_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ81251.1"
                     /translation="MLKNTALACLEQENIAVACEVNILLTDDAAIKEINAQFRNIDAP
                     TDVLSFPIANISKGKITDFGRDSDPDEGLLVIGDIVISTETALRQSDQYGHSFERELA
                     FLAAHGMYHLLGYDHEEEQDEKEMMEKQEAVLSKLGLRRA"
BASE COUNT          782 a          934 c          746 g          970 t
ORIGIN      
        1 gcagacgcgt acgtttgagg ggcgtatggg caaccgtatg ggttcaagtc ccatcgaccg
       61 caccaaaagt aaaacccacc tggattacca ggtgggtttt ttcaattacg atgacattcc
      121 tctaccccgg agcactgttc ctggcagagg ggcaactcct cacaactacg cggacattac
      181 tctactgtat ggcattgctt ccttgcgagg agtgtccccg cacaattacg gggacattcc
      241 tctgccacag ggcattgctt ccttgcagag gagtgtcccc gcacctatat attcttcaaa
      301 aaatccagct tggtaaaatc acgctccagg cgctcgcgca gatatcgcct ggttatcctt
      361 gaaatctcgt ccaggacctc cccggacaat ccaaaactga acagttcttt catcggtgcg
      421 taaacgatat gctgtatcgc ccttgacgct ccgacggaca gcggtacagc ggatccgtca
      481 tgttcggcgc agccgggcct gtcgcacaaa aatccgcaat cccggaaact gaaagaatag
      541 cttttctcag gagcgtcgcc gcagaccatg caagcctgta catggggcgc aaacccggag
      601 atcgatacag ctctcagttc aaaaatactt gtgatcaggt caggttcttt tgaagttttg
      661 gcaagcatat gcagtgaatt gagaaagagt ttcagcagtt tcggcgaagg ctggttttcc
      721 tgtatcattt ccagtactat atccgtgaag tgcgccgcat atgtaagctt aattacatcg
      781 ttccgtatct catagaatgg ttctatcact tcacagctgt ttacgctgta tatatccctg
      841 cctttgaaca gaacgaaatc accataacag aatacctgag aaccggcaga aagcctggac
      901 ctgggccgcc ttccgccttt cacaagggct gagatgcggc cctgtcccct tgtcagtata
      961 gttattatct tatcggcttc tcccgtactg acctctttta cgactatccc ctcagtttta
     1021 agaaaactca tttccatatg aactgggcag ctaaccgggt tggcctacgc ctcgctcgta
     1081 cttgtcgcgg cttcggcttc agccagtctc ctctgttccg ctgccctttc cctttccttc
     1141 tcttttattt cacggtagaa aatataagac tccaggatcc ctttacttac gaaagtattc
     1201 caggcaaaat ccttcagcat tgcaatcccc ctctgtcctt atcgttggta tctttagata
     1261 tctttgaccg gaactgcatc cgcctgtagt gtcagcgttt caaaaacagt tcctctttat
     1321 atcattctcc aaaaaagggg ttgtatactt gcaatattta cagtgccgta aagccgcatg
     1381 cttcattgat agcccagcgt tctgagcatg ctgctgctgt tgcgccagtc aggcttcact
     1441 ttcacccaca gcttcaggaa cacttttgcg ccaagcaggt tttccatctc gacccttgca
     1501 agggtcccga tcttcttcag catcgctccc tgcttcccta ttatgatgcc tttatgcgac
     1561 tcccgctcgc agtatatgtt cgcctctata tcgataatgc cctttccctc ccgttccctg
     1621 aaagaaacga tctcaacgct ggtaccgtgc gggacctcgt ccgacacaag ctcaagtatt
     1681 ttctccctta tgagttcggc cgcaagcgtc ctttccggct ggtctgtgat cacatcgtcc
     1741 gggaagtact tcgggccttc aggcagcagc tttttgatct catctattat aatgtccgtt
     1801 ccctcgccgc ttgccgcaga aatcgggata accgcactga agtccatcag ctgcgaaaag
     1861 ttcctgatcg tctccagcag ttcttctttc ttcaccagat ctatcttgtt tattattagg
     1921 atcaccggag ttttcaccgt tttgagctgc tcagctatat gcagatcccc cgcgcccggt
     1981 atcttgtccg tagcttccgc caggaaaagt atcaggtcca cttcgttgag cgtgctttcg
     2041 gcaagcttga ccatgtactc gcccagcttg gttttgggtt tgtgtatgcc aggcgtgtct
     2101 atgacgatta tctggctgtc actagtgttt attatggctt ttatggtatt gcgtgtcgtt
     2161 tgcggtttgc tcgaaattat cgacagtttc tcgcctatga gcctgttcgt aagcgtcgac
     2221 ttgccgacat taggccgtcc tataatcgaa acgaatccgg atttaaacat accatacctc
     2281 cttgctaaaa gtctgactgt ctatgaaaaa atctggaata aaagcaatgt tacaaggaaa
     2341 ccgatcactg ctcctgccat cagttccagt atattatgta ttttaccctc aagccggctc
     2401 tgtacaacaa gcaatgcgac tattatgaat aaaatcgtta ttcccgcatt gttcgaataa
     2461 agcgcgaccg ccgtcgcggc tgaaaaggct attgctgagt gcccgcttgg cattccgccg
     2521 ctgaacggcg ttcctctttt gaatacaagt ttgagcacca gcactgctgc catcgtaagg
     2581 acaagggcaa taacggtaag atgcatcggc gcctgtttta tccttgagat accggattcg
     2641 aggcttgtgc ttaccctgtc gaaaaatatg aaatatgcca cgacaagcga cagcagcgcg
     2701 gatacaagga ctgcgcctgc cgccgtatcc ttgaccgtct tcgccctggg atgataaatc
     2761 cctatcagcg tatcgatcag cacttctatt gccgtgttga aaagttcaca tataatgaca
     2821 agggcgatcg tcatgcagac gattatgaac tctatcctcg tcaggtcata aaaaagagac
     2881 agacccagca ccagtgctgc agcaatgatg tggatcttca tattgcgctc gcttttgacc
     2941 gctgaaaaaa tgccgtttat agcattgttg aagctgtcta tcaggctttt gtttttcatg
     3001 ctctccttaa ccccagtttg ctcaggacag cctcctgttt ttccatcatt tccttctcat
     3061 cctgttcctc ttcatggtca tatcccagca gatggtacat gccatgggcg gcaagaaagg
     3121 ccagttccct ttcgaaggaa tgcccgtact gatccgactg ccttaatgcc gtttcagttg
     3181 aaatcacgat gtccccgatc accaggagac cttcgtccgg atctgaatcc ctgccgaaat
     3241 cggttatttt tccctttgat atgtttgcta tgggaaacga caggacgtcg gtaggcgcat
     3301 ctatgttcct gaactgggca tttatctcct ttatcgccgc atcgtccgtc agaagtatgt
     3361 tcacttcaca ggcgaccgcg atgttttcct gctccaggca ggccagcgca gtatttttga
     3421 gtatagttcc at
//
LOCUS       DAOSCC010000238         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_17982_length_3374_cov_3.760169, whole genome shotgun sequence.
ACCESSION   DAOSCC010000238 DAOSCC010000000
VERSION     DAOSCC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17982_length_3374_cov_3.760169"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..235)
                     /locus_tag="PLG72_10420"
     CDS             complement(65..235)
                     /locus_tag="PLG72_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81252.1"
                     /translation="MRKITVIYINKKKKNIHIKQLLASLKTARNKKAIILIALLIPLI
                     FLVILLFSHLDT"
     gene            552..2216
                     /locus_tag="PLG72_10425"
     CDS             552..2216
                     /locus_tag="PLG72_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ81253.1"
                     /translation="MYIKFLRTGIAALLALAALFAGGFRYSVQAGSLQQAFPAGSEQA
                     KHFNMTYIYFGDPAAYAGHVDNAQGSLDHVSPSYFDLNGDGTLKLTKSMDRNFIDSMH
                     ERGIMVTPFLSNHWDRETGMNALEMRELLAVQIAEAVEEYDLDGVNVDIENMTEAERG
                     KYTDFVRILRRMLPSDKIISVAVAANPFGLTAGWHASYDLAALSQYCDYLMLMAYDQH
                     YEGYINSTGGAGPVAGYGFVEESIKAALKEVPADKLVLGVPFYGRLWKRGAAYGGYGI
                     SNNTVEELVRKFRGSVVFDEKQGSPRAVITIKPSDRKPVIFGVRLEAGTYDIWYENEA
                     SIKRKLELVNKYDLKGTGSWSLGQETKETWDYYHLWLEGIYFSDIQRHWAKASIAAAV
                     QNGWMNGVSADRFEPESQMTRSQAAALLVRVLGIDTSASAADAPMFIDTAGHWAQKEI
                     DAAAGAGILVGVGGGRFEPDKPITREQLAVLLDRLLGSDAGGYSSSVKFSDIDPSQVW
                     SYEAICRMAAKGFIKGFNDGTFRPRDNVSRGQMAAIIERIAPYLIK"
     gene            2714..>3374
                     /locus_tag="PLG72_10430"
     CDS             2714..>3374
                     /locus_tag="PLG72_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOJ81254.1"
                     /translation="MFKTITVMKFRGGIDPGNHKDTAELETVRMPLPPRVIIPMLQHV
                     GAPCVPLVRKGDRVFVGQKIGDSDRPISAPVHSSVSGTVTDVRPIVYSNGMEVMAVEI
                     KPDGLQELHESVAPPDCTDRESMLKAIRESGIVGLGGAGFPTSVKLSPPPGKTIDTLI
                     INGAECEPFITSDYREMMENPQYIIEGTKKVMEITSAGKAYIGVEDNKQDAIDLLSSM
                     VT"
BASE COUNT          888 a          744 c          917 g          825 t
ORIGIN      
        1 gtgaattata aagcgataca gcagataccg cagtataaca taataaaata ccaggaatat
       61 tggatcacgt atccagatgg ctgaacagga ggataaccag gaaaataaga ggtatcagca
      121 gggcgatcag tatgatcgct tttttgttgc gcgcagtttt gagcgatgcc agtagctgct
      181 ttatgtggat gttttttttc tttttgttta tgtaaatgac cgttatcttt ctcatggtaa
      241 attatattca tgttttttca tattatttct ctgataacgg gtatccctga gccaacagac
      301 ttccggtttc atggccggta tccgccagga tcccgtattt ccgccgtgcg ataccctgtt
      361 tctgcctaat gactgaattt ggttaacata atttgcttat tgatttacag ccatgcatac
      421 tgtatcttaa taaacaggtt tgatctgctg caggcaggct gagtccggga tctgaaggac
      481 acagatccct gcatttgcag aacggattaa agacatgtcg tggtcaggag aggtcagggg
      541 gtcgttatac tgtgtacata aaatttctca ggaccggtat tgcagcatta cttgctcttg
      601 ctgcattatt tgccggcggt ttccggtatt ccgttcaggc aggaagcctg cagcaggctt
      661 ttccggccgg gagcgagcag gcaaagcatt tcaatatgac atatatttat tttggggatc
      721 cggctgcata tgcaggtcac gtagacaatg cccagggatc gctggatcac gtctcgccaa
      781 gttacttcga cctcaatggg gacggaacgc ttaagctcac aaagtccatg gacaggaact
      841 tcatcgattc catgcatgaa agaggtatca tggtgacgcc attcctgagc aaccactggg
      901 accgggaaac gggcatgaac gcgctggaaa tgagggagct tcttgctgta cagatagcgg
      961 aagctgttga agaatatgac ctggacggcg tcaatgtcga tatcgagaac atgactgagg
     1021 ctgagcgggg caaatacact gacttcgtaa ggatcctcag gcggatgttg ccttcggaca
     1081 aaatcatttc cgtggctgtt gccgcaaacc cgttcgggct gacggccggc tggcatgcat
     1141 catatgatct tgccgcgctt tcgcagtatt gcgattatct catgctgatg gcatatgacc
     1201 agcattatga gggatatatc aattctacag gaggagccgg accggtggca ggatatggat
     1261 ttgtggagga gtccataaag gctgcactga aggaggttcc tgccgacaag cttgtgctcg
     1321 gcgtaccttt ctacggaagg ctctggaagc gcggtgcggc atatgggggt tacggcatca
     1381 gcaacaacac tgttgaggaa ctggtcagaa aattcagggg cagtgtcgtt ttcgatgaaa
     1441 agcagggttc gcccagggct gtcataacca taaagccttc tgacagaaag cctgtcatat
     1501 tcggtgtcag gcttgaagca ggaacatatg atatctggta tgaaaacgaa gcgtccatca
     1561 aacgcaaact tgagcttgtg aataaatacg atctcaaagg aacagggagc tggagcctcg
     1621 gacaggagac gaaagaaacc tgggactact atcatctgtg gctggaaggc atatatttca
     1681 gcgatatcca gcggcactgg gcaaaagctt cgatagctgc agccgtgcaa aacggctgga
     1741 tgaacggtgt atccgcggac aggttcgagc ctgaatccca gatgacgagg tcacaggccg
     1801 ccgccctgct cgtgagggta cttggaatcg atacttcagc gtcagctgcg gatgccccga
     1861 tgttcattga tactgcaggg cactgggcac agaaagagat cgacgcggct gcaggggccg
     1921 ggatactcgt tggtgtcgga ggcggcaggt tcgagccgga taagcccatt acccgtgagc
     1981 agttggctgt gctgctggac aggctgctgg gcagtgacgc cggtggatat tcctcttctg
     2041 tgaaattcag tgatatagac ccgtcgcagg tatggtcata tgaggcgata tgcagaatgg
     2101 ctgcaaaagg cttcatcaaa ggcttcaacg acggcacatt cagaccgcgc gataatgtca
     2161 gccggggcca gatggcggcg attatcgaaa ggatcgcacc atatctcatt aaataacggg
     2221 ccgccggttg tttccaaggc gccgcctatt tcatgaaaag tcatgtatta tatcaaatca
     2281 tggacagtcc acgctgttac aggtgtatgg actgttccgt atttcagttc gatcggaaat
     2341 caaatctgta tttaagtaac ctgtgattca ccagtacctg atcttaccct gaaaactgcc
     2401 ggacgacctg catgtaccgg ccatgaagag cccggaacgg ccttgaatta tcaggcatcc
     2461 caagtaaatt taacaagcag gaaattttga agtccaggca tggagcaggt tatctgtatt
     2521 ctatgtgtat tttgtataca attgttaaaa atttaattat gctggcgcac gtgtcctata
     2581 aatgatagaa taggtagggc gaacgagata gtacatgtca ttgacgtgaa cttctttttt
     2641 gacaactcat ttcagtcgtt gacatccggg gcgctaaacg agtcagcacc attgttttgt
     2701 gaggtgattt tttttgttca aaaccataac tgttatgaaa ttcaggggcg gcatagaccc
     2761 cggaaatcac aaggatactg ctgaacttga aacggtgaga atgccgctgc ctcccagggt
     2821 tatcattccg atgctgcagc atgtcggtgc gccatgtgtc ccgctggtaa gaaaaggtga
     2881 cagggtattt gtaggccaga agataggaga ttcggacagg cctatatctg ctcccgtcca
     2941 ttccagtgtg tccgggacag tgaccgatgt aaggccgatc gtgtactcaa atggcatgga
     3001 agtcatggca gtcgagataa agccggacgg gctgcaggaa ctgcatgaga gtgtggcccc
     3061 gcctgactgt accgacaggg aaagcatgct gaaagcgata agggagtccg gcatcgtcgg
     3121 cctcggcggc gcaggtttcc cgacaagcgt caagctttcg ccgcctcccg gaaaaaccat
     3181 tgatacactt ataatcaacg gcgcagaatg cgagccgttc ataacatcag attacagaga
     3241 gatgatggaa aatccgcaat atatcataga aggcacgaag aaggtaatgg agatcaccag
     3301 tgcagggaaa gcatatatag gcgtcgaaga caacaagcag gatgccatag acctgctatc
     3361 ctccatggtg acag
//
LOCUS       DAOSCC010000239         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18427_length_3315_cov_3.969018, whole genome shotgun sequence.
ACCESSION   DAOSCC010000239 DAOSCC010000000
VERSION     DAOSCC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18427_length_3315_cov_3.969018"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLG72_10435"
     CDS             complement(<1..510)
                     /locus_tag="PLG72_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HOJ81255.1"
                     /translation="MAIFREYDPCGRERSAEDRRRHKELVEESIKRNLGNIISEESII
                     GQSGNKKIKVPVKGIKEYSFIFGSNRKGAATGDGDERRGDVVGDDSDGNIYGYGGAGN
                     IEGEDIYETEITIEELVGYLFDDLNLPDLDRKKLSQFETVKGYRKLGYQRKGIPPRLA
                     VRRSVVEKIK"
     gene            complement(555..2480)
                     /locus_tag="PLG72_10440"
     CDS             complement(555..2480)
                     /locus_tag="PLG72_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HOJ81256.1"
                     /translation="MFNGDISEIIEKDRQERIMDRFEGTFLDYVEIVRENPEVAMLAH
                     QRIYNLISKAGFEVVKTDEDPRLRRIYGNDIIKKYKFFENFYGIDKTIMKIVRYFHSA
                     AMAGEESRQVLYLVGPVGSGKSSIMEEIKRALEASQEIYALKGCPMQEEPLHLVPYKL
                     RSRFEEILNVKIEGDLCPVCRYRLKNEYGGRYEEFPVIKTGFSVSARRGIGVVPPVDP
                     NNQDTSVLIGSVDISKIDLYPEDDPRVLSLNGAFNVGNRGVVEFIEVFKNETEYLHAM
                     ITATQEKYIPSPGKGPMIYFDGVILAHSNEAEWNKFKSDHTNEAILDRIVKIEIPYCL
                     ELNEEIKIYEKILRKSRFRAHIAPHTIEVASMFAILTRLAPSSKVDLMTKLKIYNGEE
                     IVEKGMTRKIDIAELREEAPREGMTGISTRFIMKALDMALSESEYDCINPISVMEMLI
                     RSVKELSISEEEKNKYIRFIQDSVKKEYNKILEKEITKAFIYGYREQAESLFNNYLDH
                     AEAYVNRTRIKDPNTGEELEPDEKFMRSIEEQIGITEASAKGFRADVTAYMFFVMRNG
                     GKLDYCSYEPLKEAIEKKLTASVKELSRIITKAKVRDEEQNRKYNTMVAEMKRNGYCD
                     HCCNVVLKYSANNLWKD"
     gene            complement(3064..>3315)
                     /locus_tag="PLG72_10445"
     CDS             complement(3064..>3315)
                     /locus_tag="PLG72_10445"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312425.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOJ81257.1"
                     /translation="AVMFARSEGIVPAPESSHAIRAAIDEAVKAKEAGEEKVILFNLS
                     GHGYFDKSSYDLYFSGNMKDVEYSRENVEKSIARLPKVK"
BASE COUNT          716 a          902 c          679 g         1018 t
ORIGIN      
        1 cttgatcttc tctacgaccg atcgcctgac cgccagcctc ggagggatgc cttttctttg
       61 atatcccaac tttctgtaac ctttgactgt ctcgaactgg gagagtttct tcctgtccag
      121 atccggcagg ttcaggtcat cgaacagata acccacaagc tcttcaatag tgatctctgt
      181 ttcgtatata tcctcgcctt cgatattgcc ggcgcctccg taaccgtaga tgttcccgtc
      241 ggaatcatca ccgaccacgt cgccgcgtct ttcatctcca tcgcctgttg cagcgccctt
      301 cctgttgcta ccgaaaataa aactgtactc ctttatacct ttcacaggca cttttatttt
      361 tttgttgccg ctctggccga taatgctttc ttccgatatt atattgccaa gattcctttt
      421 gatcgattcc tccaccaact ctttatgcct gcgcctgtct tcggcagacc tctctctccc
      481 gcaggggtca tattctctga aaatcgccat attttcgtcc tcttatgatc agatggtttg
      541 gagccttcac ggtttcagtc tttccagagg ttatttgcac tgtacttgag cacgacgttg
      601 cagcagtggt cgcaataccc gttgcgtttc atttccgcaa ccatcgtgtt gtacttcctg
      661 ttctgttcct cgtctctcac ttttgccttc gtaatgatcc tgctgagttc cttcacagat
      721 gccgtgagct ttttctcgat ggcctcctta agaggttcgt aactgcagta gtccagcttt
      781 ccgccatttc tcatcacaaa aaacatgtat gcggtaacat ccgccctgaa gccctttgca
      841 gacgcttccg ttatccctat ctgctcctcg atggacctca tgaacttttc atcgggttca
      901 agctcttcgc ccgtatttgg atcctttatc ctggttctgt tcacatacgc ttccgcatgg
      961 tcgaggtaat tgttgaaaag gctttccgcc tgttctctgt aaccgtaaat gaaagccttg
     1021 gttatctctt tttcaaggat tttgttgtac tccttcttca ctgaatcctg tatgaacctt
     1081 atatatttat ttttctcctc ttcactgatc gacagttcct ttaccgacct gatcagcatt
     1141 tccatgactg aaatggggtt tatgcagtcg tattcggatt ccgacaatgc catgtccagc
     1201 gccttcatta tgaatctcgt ggaaatgccg gtcattcctt cacgaggcgc ctcttccctg
     1261 agttccgcta tgtcgatttt gcgggtcatg cccttttcaa cgatctcttc gccgttatat
     1321 atcttgagct tcgtcataag atccactttc gacgacggag caagacgtgt cagtatggca
     1381 aacatcgagg ctacctcgat tgtatggggc gctatgtggg ccctgaacct gctttttctc
     1441 aggatcttct catatatttt gatctcttcg ttcaactcga ggcagtacgg gatttctatc
     1501 ttcactatcc tgtcaaggat cgcttcattg gtatgatctg atttgaactt gttccattca
     1561 gcctcgttcg agtgggcaag gatgacgccg tcgaaataga tcattgggcc tttcccgggc
     1621 gagggtatat atttttcctg ggttgccgtt atcatcgcat gcagatactc tgtctcgttt
     1681 ttgaacacct ctataaactc gacaactcct ctgttgccga cgttgaacgc tccgttcagg
     1741 gacagtaccc ttggatcgtc ctccggatac aggtcgattt tcgaaatgtc gacacttccg
     1801 atcagcacgc tcgtatcctg gttgttcgga tcaacgggcg gcactacgcc gatccctctc
     1861 cttgcactga ccgaaaatcc tgtcttgatt actgggaatt cctcgtatct cccgccgtat
     1921 tcatttttga gcctgtatcg gcaaacaggg cacagatcgc cttctatctt cacattgagg
     1981 atctcttcga atctgctcct tagtttataa ggcacaaggt gaaggggttc ttcctgcatc
     2041 gggcatcctt tcagcgcata tatctcctgg ctcgcttcaa gggcccgttt tatctcttcc
     2101 atgatagagg atttcccgga cccgaccggt ccgacgagat ataaaacctg cctcgattcc
     2161 tcgccggcca tggccgctga atggaaatac ctcactatct tcattatggt tttgtcgatg
     2221 ccgtagaagt tctcaaaaaa cttgtatttc tttattatat cgttgccgta gatccttctc
     2281 agcctcgggt cttcatctgt cttaaccact tcgaagcctg cttttgaaat caggttgtag
     2341 atcctttggt gtgcaagcat agcaacttcc ggattctccc tgactatctc cacataatca
     2401 aggaacgtcc cctcaaaacg gtccatgatc ctctcctgtc ggtccttctc tattatttcg
     2461 gaaatgtcgc cgttgaacat ctcgcctgcc tccttctcct gcaaaacccc tgaaccggta
     2521 cccacggatc caaccctggt ctgcctccgt cagcagccac atttaaatta tattagcgct
     2581 cggcagctaa aataccacgg actgccatgg ggacagttct tcagaagcct gcttggggac
     2641 agtcctgcag aagttaagca tggggacagt tctgcaaatc aaattaattt ggggacagtt
     2701 cttcagaagt gctcatcata tgaaagtcag gcttttactg ataagcaagg tccaaatgaa
     2761 tacaaggatt taattcaggt tcgttaataa ggtatacctt atacacaaac ctgatatatt
     2821 cagcaaagat ctatgaagcc gagttattcc cggacataag tagtattcgc ataaacttga
     2881 gaactgtccc caacgacccg acgacaaaac ttgagaactg tccccagccg gctcttccac
     2941 tgaaaagtgc agaactgtcc ctgccgccaa ctaatgaaga actgtcccca actccgccgg
     3001 cgcatcaaaa aagctgccgt cggcagaaat gacggcagct tgcggattta cagcggtata
     3061 atgctatttt actttcggca gccgggctat gctcttttcg acattttccc ttgaatattc
     3121 tacgtctttc atattgccgc tgaagtataa gtcgtatgat gacttatcga aatatccatg
     3181 gccgctcagg ttgaacagta tcaccttttc ctcgccggct tcctttgctt tgacagcttc
     3241 atcaatagct gcgcggatag cgtgtgagga ttcgggagca gggacgatac cctctgatct
     3301 tgcaaacatg acggc
//
LOCUS       DAOSCC010000240         3310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18469_length_3310_cov_3.325038, whole genome shotgun sequence.
ACCESSION   DAOSCC010000240 DAOSCC010000000
VERSION     DAOSCC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18469_length_3310_cov_3.325038"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLG72_10450"
     CDS             complement(<1..404)
                     /locus_tag="PLG72_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HOJ81258.1"
                     /translation="MKRSKENGIAVIPDIKCKSPAEGDLIPEDDAPDLAAELVEAGAP
                     VISVVTESEHYGGSAKLLERIARSAKAPVLRKDFIKCRRQLEESVEMGASAVLLIASM
                     LEKDTLARLAEDALMLGLEPLTEIHGFEDMEKT"
     gene            complement(442..1488)
                     /gene="trpD"
                     /locus_tag="PLG72_10455"
     CDS             complement(442..1488)
                     /gene="trpD"
                     /locus_tag="PLG72_10455"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOJ81259.1"
                     /translation="MGKSLIKDVLARLTAKEDLTTEEIYGLIEAIRNDELSDTQIAGF
                     QVALLMKGPSLAEITAIAAAMRDNCIRIDPDVDEELMDTCGTGGGLSTFNISTAVAFV
                     AAAAGIPVAKHGSRSISSLSGSADVLEALGVEFNQSPRGVEKLIEDVGIAFLYAPLFH
                     PVMCKVLPPEKDLGIKTIFYTVIGPLINPSKASRHLLGVYKPELLDTVTHVAASIGYK
                     RALFVHGLDGLDEISLLGKTRINELDNGSITTYEIEPEDFSLERCSLEDIATGTPQEN
                     AAMIDDVFSGRDKGPRRTAVVLNAAGALMVGGRAGSFREGIGLAGELIDSGEAQKKLK
                     RLVEASHEYRRLYS"
     gene            complement(1725..2498)
                     /gene="trpA"
                     /locus_tag="PLG72_10460"
     CDS             complement(1725..2498)
                     /gene="trpA"
                     /locus_tag="PLG72_10460"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ81260.1"
                     /translation="MSRLDRVFSRGKAFIPFITAGDPSITVTEQLIYSMYEAGADLIE
                     LGIPFSDPVAEGPVIQAADERALAGGVTVDDVFTMMQRLRNSCDVPVALMTYVNPVAT
                     YGTERFMINCSRSGIDAVIIPDVPFEERDELLPYCRKHGITLIPMIAPTSGERIRMIA
                     AEAEGFVYCVSSLGVTGMREKIGHEVSGMIKAVKNVKDIPCAIGFGISTPEQAAELSC
                     YGDGIIVGSAVVKIVEEYGDKCVEHVAEYVRKMKAAICG"
     gene            complement(2485..>3310)
                     /gene="trpB"
                     /locus_tag="PLG72_10465"
     CDS             complement(2485..>3310)
                     /gene="trpB"
                     /locus_tag="PLG72_10465"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ81261.1"
                     /translation="LMGMECEIHMGKEDTERQALNVYRMELLGAKVHAVTTGTQTLKD
                     AVNEAMREWTRRISDTHYVLGSAVGPHPFPTMVRDFQKVIGQEVKQQLAEKEGRLPDV
                     VLACVGGGSNAIGIFHDFIDDGGVRLIGCEAAGRGIDTDKTAATMTLGSVGIFHGMKS
                     YFCQDEYGQIAPVYSISAGLDYPGVGPEHAGLRDRGRAEYVPVTDDEAVDAFEYLSRT
                     EGIIPAIESAHAVAYAKKIAPEMARDRIIVICLSGRGDKDVASIARYRGVQINEQA"
BASE COUNT          689 a          995 c          735 g          891 t
ORIGIN      
        1 gtcttttcca tatcctcaaa gccgtggatc tctgtcagag gttccagtcc gagcatcagt
       61 gcgtcttccg ccagcctcgc caatgtgtcc ttctccagca tcgacgctat cagcaggacg
      121 gcagacgcac ccatttcaac gctttcttcc agttgtctgc ggcattttat gaaatccttc
      181 ctgagcaccg gcgcttttgc cgaccgtgcg atccgctcca ggagctttgc cgaccctccg
      241 taatgctcgc tttccgtgac cacggatata acaggcgctc ctgcctcaac aagttctgcg
      301 gccagatctg gggcatcgtc ttccgggatg aggtctccct ctgcaggcga cttgcatttg
      361 atatcaggta tcactgctat tccgttttct ttagatctct tcattaatat atttgtaaat
      421 gtaccgtacg gaaaggctct gtcaggaata taaacgcctg tactcatgtg aagcctccac
      481 aagccttttc agttttttct gcgcctctcc gctgtctatc agttcgcctg caagcccgat
      541 gccttccctg aaactgcccg ccctgccgcc gaccatcagt gctccagcgg cattcagaac
      601 gactgcggtc cttctcggtc ctttatctct tcccgaaaaa acatcatcga tcatggcagc
      661 attctcctgg ggggtcccgg tcgcgatatc ttcaaggctg cagcgctcaa ggctgaaatc
      721 ttccggctcg atctcatatg tagtgatgct gccgttatcc agttcattta tcctcgtttt
      781 ccccaaaaga gaaatctcgt caagaccgtc aagcccgtga acgaaaaggg ctcttttata
      841 tccgatggaa gctgcgacat gcgtgaccgt atccagcagt tcgggtttgt agacacccag
      901 taagtgcctg gaagctttgg acggattgat aagcggacct atcactgtat agaagatagt
      961 ctttatacca agatcctttt cagggggaag gaccttgcac atgaccggat ggaacagagg
     1021 tgcatacaga aatgcgatgc ccacgtcttc aataagcttt tcgactcctc tcgggctttg
     1081 gttgaattca acgccgagtg cttccagtac atcggcgctg ccggaaaggc tggatataga
     1141 ccggctcccg tgctttgcca ccgggatgcc ggcagcggcc gcaacgaaag cgactgctgt
     1201 ggaaatgttg aaagtactga gtccgccgcc ggtgccgcag gtatccatca gttcctcgtc
     1261 cacgtcagga tcgatccgga tgcagttatc cctcatcgcc gcggctatgg cagttatctc
     1321 cgccagggac ggccctttca tcaacagcgc cacctggaag ccggcgatct gcgtatcgct
     1381 gagttcgtcg tttcttatag cctcgatcag accatagatc tcttccgttg tcagatcttc
     1441 ctttgctgtc agtcttgcga gtacatcttt gatcagactc ttacccattc tcatctctcc
     1501 tcttctcatc atttctctga tatattttcg atttattaca gctgtttaca gcctgtaatc
     1561 tcatggtagt catatggctc cgtgataata cctgtgtaag cctgatacac tttcaaaacg
     1621 gccgatatgt ctcaatatct gattcatttt cgtatccgat gtgttttcgg cttataacgg
     1681 ggcaagcggc cgcctggtgc cggcagtgct gaaaagctct gcactcaacc gcatatggcc
     1741 gctttcatct ttctgacata ttccgcaaca tgctcgacac acttatcgcc gtattcttcc
     1801 actattttta ccactgcgct tcccacgatg atgccgtcac cgtaacagga aagttctgca
     1861 gcctgttccg gcgtcgatat gccaaatccg atggcgcatg ggatatcttt cacatttttg
     1921 acggctttta tcatcccgct tacttcatgt cctattttct ccctcatgcc ggtcacaccc
     1981 agtgaagata cgcagtatac aaagccttcc gcttcggctg ctatcatcct gatgcgttca
     2041 ccggatgtcg gcgctatcat cggaatgagt gttatgccat gtttcctgca atacggcagc
     2101 agttcgtccc tctcttcgaa agggacatcc gggatgatca ccgcatcgat acccgatctg
     2161 ctgcagttta tcatgaacct ttccgtgcca tatgtcgcca cgggattgac gtatgtcatc
     2221 aatgcaactg gtacatcgca gctattcctg agcctctgca tcatcgtaaa aacgtcgtcc
     2281 acggtaacgc cgccggcaag cgcccgctcg tcggcagcct gtatcacagg cccctcggcc
     2341 acaggatccg aaaacggtat gcccagctct atcaggtctg cgcccgcctc atacatactg
     2401 taaatcaatt gctccgtgac agttatcgaa ggatctccgg cagttatgaa aggaatgaac
     2461 gcctttccac gtgaaaaaac cctgtcaagc ctgctcattt atctgcaccc ccctgtaccg
     2521 tgctatggaa gccacatcct tgtctcccct tccggaaaga catataacga ttatcctgtc
     2581 ccttgccatt tccggggcta ttttcttcgc atatgccact gcatgagcac tctcgatggc
     2641 agggatgatt ccttccgtac gggacagata ttcaaacgca tctaccgctt catcatcagt
     2701 caccggaaca tactctgccc tgcccctgtc ccggagtccg gcgtgttccg ggccgacgcc
     2761 cggataatcc agtcctgccg aaatcgagta gacaggcgct atctggccgt attcatcctg
     2821 gcagaagtat gatttcatac catggaatat tccgacactg cccagggtca tggtggccgc
     2881 cgtcttgtcc gtatcgatcc cgcggcctgc tgcttcacag ccgatcaggc gtacccctcc
     2941 atcgtctatg aaatcgtgga aaatgcctat tgcgttgctg cccccgccaa cacaggccaa
     3001 taccacatca ggcagcctgc cttctttttc tgcaagttgc tgtttcactt cctgccctat
     3061 tactttctgg aaatccctca ccatcgttgg gaacggatga gggccgacag ccgatccaag
     3121 cacatagtgg gtatcgctta tccgccttgt ccattcgcgc atggcctcat taactgcatc
     3181 cttcagcgtc tgtgtcccgg tggtcaccgc gtggaccttc gctcccagca gttccatcct
     3241 gtaaacgttc agagcctgcc tctcagtatc ttctttcccc atatgtatct cacattccat
     3301 gcccatcagg
//
LOCUS       DAOSCC010000241         3287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18636_length_3287_cov_3.963490, whole genome shotgun sequence.
ACCESSION   DAOSCC010000241 DAOSCC010000000
VERSION     DAOSCC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18636_length_3287_cov_3.963490"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..987)
                     /locus_tag="PLG72_10470"
     CDS             complement(106..987)
                     /locus_tag="PLG72_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HOJ81262.1"
                     /translation="MRLVIGETEIKVNITAAIMPVFMLIAGLTAEYAAAFFSMLLHEL
                     SHILAARICGVKAGIVSVTILGFSAVIPDDGCSKTERFLICLAGPAFNLLFFAAALLT
                     EHLVPGDQGFLRLLSASNLFLAFINLLPSLPLDGGRLMLVFLAGNIGMLAAGRVMRVL
                     AGIVSIIVMLAGGYQLYASSYNASLIIIGLYLLLTTMTGKVENALMNIRQILFRKSRL
                     LKKGFYPARDLVVLKSTRLGDILKSMDFDRFHIVYVLDDSLRIVGTFTESEIMDALAG
                     SNDDLTFERLIEIRFQG"
     gene            complement(984..2213)
                     /locus_tag="PLG72_10475"
     CDS             complement(984..2213)
                     /locus_tag="PLG72_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ81263.1"
                     /translation="MESCMTGRGYTPRRIYPHRRLRRYSRQGRLRRFGQSRLGRVIAF
                     QALVCSILLFVAVLARVLNIGVAGYITEKVRYVLQHDMELKSIYTYAQTLASDIRSSI
                     NRDGDRTGNNGSIDGDTENDKRGNGGDIRDNPAIASTDGSLADSSDNTATLSAEMALP
                     DTMDATSGIYDEMTQSGNNWTQSGGDSDLTGDIDHDGETVPDGHEGFDGKETADSTDA
                     PQQNESETRVLAASSEIDDSAENTHDPPDNSDDSAKDTSPGQKNVVSDADKYGDFGKK
                     RGDFNMAVPVEGKVVTPFGETKGAAGMLKMHNGVDIAVGKMSGIKAVLDGRVEDAGSS
                     PGYGKFIKIRHSDSLVTVYANCSDIIAGIGEYVGKGDVIAVAGGERTAGGSHVHFEVW
                     QDGVPADPLDYISIDYR"
     gene            2425..3033
                     /locus_tag="PLG72_10480"
     CDS             2425..3033
                     /locus_tag="PLG72_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ81264.1"
                     /translation="MKNIITVQHTQSIHHTNGMIGSWTDWDLTDLGIEQAKRIGERLS
                     SEIWNKRYVMYSSDLLRAKHTAEIIAGYLNIEPVFTDLLRERNLGEAVGKSVEWAHRN
                     TIVWERTIDDKPFEGAESRRELWERISVFYRHMMENTDENVIIVSHGDTLSVFSAMWL
                     GLDVEMLNKCDLHGKAGGVSFMHENPDGKHIITRLSDLSYIR"
     gene            complement(3194..>3287)
                     /locus_tag="PLG72_10485"
     CDS             complement(3194..>3287)
                     /locus_tag="PLG72_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOJ81265.1"
                     /translation="NVPFATNIATAEMLIKGLERGDLQWREHVR"
BASE COUNT          814 a          846 c          750 g          877 t
ORIGIN      
        1 gccggtccgg agtgcccgaa gggtaacgcc cctcagcatg aaaaccatcc ccgcttctcg
       61 ggaaccgtcc ccgtgtctgc atgtcagcag gttttgctgt catgcctatc cctggaacct
      121 tatttctata agccgttcaa acgtcagatc gtcattgctg ccggcaaggg catccattat
      181 ttcgctttca gtgaacgtgc caacgatcct taacgaatca tcaagtacat aaacgatatg
      241 gaacctgtca aaatccatac ttttcagaat gtcgccgagt ctggtgcttt tcagtacaac
      301 caggtctctt gcgggataaa aacctttctt aaggagtctg gatttcctga ataatatctg
      361 ccttatattc attaaagcgt tctccacctt ccctgtcatc gtagtcagta acaagtacag
      421 cccgattatg atcaggctcg cattgtatga cgaagcatac agctggtacc caccggcaag
      481 cataacaatt atcgaaacaa tgcctgcaag cacacgcatg accctgccgg cggccagcat
      541 cccgatattg ccggccaaaa acacaagcat aagcctgccg ccgtccaggg gcagtgacgg
      601 cagcaggttt atgaatgcaa gaaacaggtt ggaagcggac agcagcctga gaaagccctg
      661 gtcgccggga accagatgct cggtcagcaa cgcagcggca aagaacaaaa ggttgaaagc
      721 aggtccggca aggcagataa gaaacctttc cgttttactg cacccgtcgt ccgggatcac
      781 agccgaaaaa ccaagtatcg tcacgcttac aataccggcc ttaacaccgc agatccttgc
      841 cgccagtata tggctgagtt cgtggagcag cattgaaaaa aacgcagccg cgtattccgc
      901 tgtaaggccc gctatcagca tgaagacagg cattattgcc gcagttatgt ttactttgat
      961 ctcagtttct ccaataacca gtctcacctg tagtcaatgc ttatgtaatc aagcggatct
     1021 gctggtacgc cgtcctgcca gacctcaaaa tggacatggc tcccgcctgc cgtacgttcc
     1081 ccgcctgcga cagcgattac atcccctttt cccacgtatt cgccaatacc ggcaataatg
     1141 tccgaacaat ttgcatatac cgttacaagg ctgtcgctgt gtcttatctt tataaacttc
     1201 ccgtatcccg gagaagaacc tgcatcttca acgcgtccgt caagcacggc ttttatgccg
     1261 ctcattttcc cgactgcgat atcgacgccg ttgtgcattt tcaacatgcc tgccgcccct
     1321 tttgtttcgc cgaatggcgt tacgaccttt ccttccacgg gaacagccat gttgaagtcc
     1381 ccgcgtttct ttccgaagtc cccatactta tcggcatctg acacaacatt tttttggccg
     1441 ggtgatgtgt cttttgccga gtcatcgcta ttatccggcg gatcatgggt attttccgct
     1501 gaatcatcaa tttcgctgct agctgcaagt accctggttt cagactcatt ttgctgcggt
     1561 gcatctgtgc tgtcggcggt ttccttgccg tcaaacccct catgtccgtc aggtacagtt
     1621 tcgccgtcgt gatcaatgtc gccggtcaaa tcgctgtcgc cgcctgactg agtccaatta
     1681 ttaccggact gcgtcatttc atcatatata ccggaagttg catccattgt atcaggcagt
     1741 gccatttctg ctgacagagt ggctgtatta tccgaactgt ctgccaggga accgtctgtc
     1801 gaagcaatcg caggattatc ccggatatcg ccgccgtttc ctcttttatc attttctgta
     1861 tctccatcga tactgccatt attgccggtt ctgtctccat ccctgttaat actgctcctg
     1921 atatccgaag caagcgtttg cgcatatgtg tagatgcttt tcagttccat gtcatgctgc
     1981 agcacatacc ggactttttc tgttatatac ccggcaacgc cgatgttgag gaccctggcc
     2041 aggacggcaa caaacagcag tatcgagcag acaagtgcct ggaatgcaat gaccctgccc
     2101 agcctgctct gcccgaacct tcgcagcctg ccttgcctgg aatatcttct gagccttctg
     2161 tgcggatata tgcgccgcgg cgtgtatccc ctgccggtca tacagctttc catatcgttt
     2221 catctccggt attgttgtta tacagccctg gtaaattata ttgatttaaa cggccattta
     2281 tgtatacaca gtagattcgg ataaatgcat atacagtgct acattggcaa aaccagttgg
     2341 tattttggat tgttaaacct tgtgataaaa tatgcatatg attgatccga ggtgtttctg
     2401 caatttacat acacgggagg atatatgaaa aatataatca ctgttcagca tacccagtcg
     2461 atacatcata caaatggcat gatcggttca tggacagact gggatttgac cgaccttggg
     2521 atagaacagg cgaaacggat aggtgaacgg ctttcatctg aaatctggaa caagcgatat
     2581 gtcatgtatt catctgattt gctgcgggca aaacatacag ctgaaattat agcaggttat
     2641 cttaacattg agccggtatt tacagacctg ctaagggaac gcaatttggg tgaagcagtc
     2701 ggaaaatcag tggaatgggc acatcgaaac acgatagtct gggaaaggac gatcgacgat
     2761 aagccgttcg aaggggcgga atcccgaaga gaattgtggg agcgtatttc ggttttttac
     2821 aggcatatga tggaaaatac agatgagaac gttatcattg tttcacatgg tgatacattg
     2881 agcgtattca gcgccatgtg gcttggactg gatgttgaaa tgctcaacaa atgcgatttg
     2941 cacggcaaag ccggaggcgt ttcgtttatg catgaaaacc cggacggaaa gcatatcatc
     3001 acccgactga gcgatttatc atacatccgc tgatatacat ttcagtatga actactgtgt
     3061 tgcatttata caaggattgc accaaaaacg caggcgcctg ccggccgcct gcgtcttacg
     3121 cataaaaata ttcctttctg ttgcgacaac actcctggtt gtcgtcccgt atactatgtc
     3181 cgtttcggta tgttcatctg acatgttcac gccactggag gtcgcctctt tcgagacctt
     3241 tgatcagcat ctcggcagtg gctatattgg tggcaaaagg aacattg
//
LOCUS       DAOSCC010000242         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18925_length_3251_cov_3.877034, whole genome shotgun sequence.
ACCESSION   DAOSCC010000242 DAOSCC010000000
VERSION     DAOSCC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18925_length_3251_cov_3.877034"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PLG72_10490"
     CDS             complement(<1..1452)
                     /locus_tag="PLG72_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ81266.1"
                     /translation="MKRIYILGGIFTLLFLLLAARTATLQLMPDRSLEEAAFRQRVSG
                     MGIERIRGNILDRNNIPFTNREIKYMALVKTSLMPQSEAEKEKVFRAMGVGMPEDLTS
                     KSSPLLLKIDESAGEALKNLDVDWISVICSLERYGEDSLARHVTGYLNKSDQVGMSGI
                     EKAYEDILRDNVAFEIGTVTDAVREPIKGFGYRLKDWSSDSRLNVRLTLDYHVQKIVE
                     DAMDNNRLSGAVVVEDVVTGDILAMASRPHFDQNAVEKYLDSSGKELFNKATAAYNLG
                     SVFKIIDLAAFFENEEEIMNGDAAEENDAGDRASPGEAGSLTDIYRFFDGFSYSPVPF
                     PEHIDIPAIFGRHEFTDPDNYYCSGAVEINGLTFKCYSYYEGGHGSLDLERAFALSCN
                     SYFIELGQRIGYRRLVETAQKFGLGSRTGISEQGVAESPGNLPDINAYHSRADIANIA
                     IGQGEILATPVQVADLTATVANGGIKNRVNIVDA"
     gene            complement(1551..2786)
                     /locus_tag="PLG72_10495"
     CDS             complement(1551..2786)
                     /locus_tag="PLG72_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ81267.1"
                     /translation="MRRPELLAPAGNLEKLKMAVVYGADAVYLGGEEYGLRAAAGNFS
                     DDELEEGIAFAHAHGRKVYLTMNIIPHNKDFDGMAEYIARVRDMGADAVIFSDPGVYD
                     MLRQYAPGMELHLSTQANNTNWRSAAFWHRHGVKRIILARELSIDEIREIRRNVPEDL
                     ELEIFVHGAMCISYSGRCLLSNYMASRDSNRGQCAHPCRWNYRLVEEKRPGQYFPVYE
                     NERGTYIFNSKDLCLIEHIPEIAESGISSLKIEGRMKSSYYVATVVKAYREALDAYIS
                     DPEGYTFDNAWLEEISKASHREYTTGFFYSKPTGDGQVYGSSSYIREYDFVGLVTAYD
                     ENTATATIEQRNRFAVGDELEVVPPKGPYHMQKVTEMRNADGETIEAAPHPQMTVYMR
                     MDRPVEPFTMLRRKAVSVC"
     gene            complement(2801..>3251)
                     /locus_tag="PLG72_10500"
     CDS             complement(2801..>3251)
                     /locus_tag="PLG72_10500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOJ81268.1"
                     /translation="LAGILAPGGRIDTIERDEDMVSKAHEYIKRAGLADTIAVIAGEA
                     SEVLACLDKTYDMIFMDAAKGQYPVFLPECLRMLRNGGLLITDNVLYMGLVDREGPVA
                     RKHRTIVNNMRSYIDTLRNDPSLDTSVLPVGDGVALSYKRMETEMDR"
BASE COUNT          659 a          949 c          739 g          904 t
ORIGIN      
        1 tgcgtccact atgttgaccc tgtttttgat gcccccgttg gcaaccgtgg cggtaaggtc
       61 ggcgacctgt accggcgtcg caagtatctc gccctgtccg atggcgatat tcgctatatc
      121 ggccctgctg tggtatgcat ttatatcagg cagattcccc ggtgattctg ctacaccctg
      181 ttccgatatc ccggtcctgg atccaaggcc gaatttctgc gccgtctcca ccagcctcct
      241 gtagccgatc ctctggccaa gctcaataaa atacgagttg caggacagtg cgaacgccct
      301 ctcgaggtcc aggctgccgt gccctccctc atagtaggaa tagcatttga acgttagccc
      361 gttgatttcg actgctccgc tgcagtaata gttgtccgga tcggtgaact catgacgtcc
      421 gaaaatcgcc gggatatcga tgtgttcggg gaatggcacc ggactgtagg aaaaaccgtc
      481 aaaaaacctg tatatgtcag tcaggcttcc ggcttcgccc gggctggctc tgtcacctgc
      541 atcattttct tctgcagcat ctccgttcat gatctcttct tcattttcga aaaatgccgc
      601 caggtctatg attttgaata cggagccgag gttgtaggct gctgtcgctt tgttgaacag
      661 ttccttgccg ctgctgtcaa ggtacttctc cacggcattc tggtcaaaat gcggcctgct
      721 tgccatggca agtatgtctc ctgtcacgac atcttccacg acgacagctc ctgacagcct
      781 gttattgtcc atagcatctt ctactatctt ctgtacatga taatccagtg tcagccttac
      841 attcagcctg ctgtcgctgc tccagtcctt cagcctgtaa ccgaacccct ttatcggctc
      901 ccttacggca tccgtaactg ttccgatctc gaaggccaca ttatctctaa gtatgtcttc
      961 atatgccttc tcgatgcccg acatgcccac ctggtcgctt ttgttcaaat atcctgtcac
     1021 atgccttgcc agtgaatcct ctccgtaccg ttccagcgaa cagatgaccg agatccagtc
     1081 cacatccagg ttcttgagcg cttctccggc actttcgtcg attttcagca gtaaaggact
     1141 gctctttgat gtcagatcct caggcatgcc gacacccatc gctctgaaaa ctttttcctt
     1201 ctcagcctca gactgcggca tgagggatgt ctttaccagc gccatgtatt tgatttccct
     1261 gtttgtgaac gggatattgt tccggtcaag tatgttgccc ctgatcctct ctatacccat
     1321 gccggacact ctttgcctga acgcggcctc ctcaagcgac ctgtcaggca taagctgcag
     1381 cgtggctgtc cttgcggcaa gaagcaggaa caggagtgta aaaatgccgc caagtatgta
     1441 gatcctcttc actgacaagc aaataacccc tttcaacggt gataccaaga ttcttgccgt
     1501 cgcaaagggg ttatattcat catgacgtgt tgcggtattt tatatcagtt tcagcaaaca
     1561 ctcacagctt ttcgcctcag cattgtaaac ggttcaacgg gacggtccat gcgcatgtat
     1621 acagtcatct gcggatgtgg ggcggcttct atggtttctc cgtcagcatt ccgcatttcc
     1681 gtaacttttt gcatatggta tggccccttt ggagggacta cttcaagctc gtctcccact
     1741 gcgaacctgt tgcgctgttc gatggtggct gtggccgtat tttcgtcata agccgtcaca
     1801 agaccgacaa aatcatactc ccttatatat gagctggatc cgtatacctg gccgtcgccg
     1861 gtgggtttgc tgtaaaagaa acctgtcgtg tactccctgt ggctggcctt tgatatttct
     1921 tcaagccatg cattgtcgaa ggtgtatccc tcagggtctg aaatatacgc atccagtgct
     1981 tccctgtatg ctttcacgac tgtagcaaca taataggagc ttttcatcct tccctcgatc
     2041 ttgaggctcg agatcccgct ctccgctatt tcaggtatgt gctctatcag gcacagatcc
     2101 tttgaattga atatatatgt gcccctttcg ttctcataga cagggaagta ttgacccggc
     2161 cgcttttcct cgaccaggcg gtagttccat ctgcaggggt gtgcgcactg ccccctgttc
     2221 gagtccctgc tggccatata gttgctcagc aggcaacgtc cggaataaga gatgcacatc
     2281 gccccgtgga cgaaaatctc gagttccagg tcttccggca cgtttctcct tatctccctg
     2341 atctcatcga tcgagagttc ccttgccagt attatccttt taactccatg cctgtgccag
     2401 aatgcagcgc tcctccagtt tgtgttgttc gcctgcgtgc tgaggtgcag ttccatgcca
     2461 ggcgcatact ggcgcaacat gtcgtaaaca ccgggatctg agaatatgac cgcatccgct
     2521 cccatatccc taaccctggc aatgtactcg gccataccat caaaatcttt attatggggt
     2581 attatattca tcgtgagata gactttcctg ccatgggcat gggcaaaagc gatcccctct
     2641 tccaattcgt catcggagaa attgcccgcc gccgccctga ggccgtactc ctcccctcca
     2701 aggtaaacgg catcggctcc atatacgacg gccattttca gcttttcaag gttcccggcc
     2761 ggagccagca gttcaggcct tctaatggtt ttgctcctgt tcatcggtcc atctccgttt
     2821 ccatgcgctt gtaagacaat gccactccgt ccccgacagg cagcacgctc gtgtccagcg
     2881 acggatcatt gcgcagcgta tcaatgtatg agcgcatatt gttcactatc gtcctgtgct
     2941 ttcttgccac aggcccttcc ctgtccacca ggcccatgta cagaacattg tcggttatga
     3001 gaaggcctcc gttcctgagc atcctcaggc attccggcag aaatacggga tattgcccct
     3061 ttgccgcatc catgaatatc atatcatatg tcttatccag gcacgcgagc acttccgaag
     3121 cctctcccgc tatcacggct atagtgtctg ccagccctgc ccttttgatg tactcatgtg
     3181 ctttggaaac catgtcttca tcgcgttcga tggtatcgat cctgccgccg ggcgcaagta
     3241 ttcccgcaag c
//
LOCUS       DAOSCC010000243         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18935_length_3250_cov_4.515493, whole genome shotgun sequence.
ACCESSION   DAOSCC010000243 DAOSCC010000000
VERSION     DAOSCC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18935_length_3250_cov_4.515493"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..418
                     /locus_tag="PLG72_10505"
     CDS             98..418
                     /locus_tag="PLG72_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81269.1"
                     /translation="MRRKNIISRAITILLVILLALLLAACGISQKDEVSGGGSQTGSY
                     GGSGSEGEAAGGIGKASGPGGSGVSVIPDSTDGAGLYYTDVPKSAETNFTIIYNEYTG
                     SRAP"
     gene            390..710
                     /locus_tag="PLG72_10510"
     CDS             390..710
                     /locus_tag="PLG72_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81270.1"
                     /translation="MNTRAAGLPEGYRADLVPVLEDSTLKKSNVLDTYESGKHYSLVF
                     ETMSGYKDVVAFYKEIMQNAESRVVSDYETCCDITGTLDGVTVHIGINLMPEGTLYLI
                     EMVI"
     gene            complement(865..1521)
                     /locus_tag="PLG72_10515"
     CDS             complement(865..1521)
                     /locus_tag="PLG72_10515"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOJ81271.1"
                     /translation="MGILTRRERYILGGKQAIMIGNYSNVEWHPLKGPDKELAEILND
                     FGVEFTEDYDRFRYDSLSRYDLCIAYTENRLTDEQVAGLLTYVLNGGGLLALHNGIVV
                     EARYEAAHLAGGRFRSHPDQKVLTYMPAGSGHVILEGIESFDIMEEPYQFDLDNLLET
                     TLLLEYESEGKKWPAAWAHNYGLGRVVYLSPGHQLASFMVPMYRNLIRRGALWAAGML
                     "
     gene            complement(1509..2228)
                     /locus_tag="PLG72_10520"
     CDS             complement(1509..2228)
                     /locus_tag="PLG72_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ81272.1"
                     /translation="MKLYSCVTGADRGLGFELAKALLEDGYTVFAGRYNKDWKLLDKL
                     SEKYPDSLYIVDLDVSSDTSVKAAAEFISSRTDRIDVLVNNSAISGNYDPTIFDELDF
                     DEMLRVFNVNCLGATRVTNALIRLIMNSEKKLIMNISSEAGSISTCYRKGWFAYCGSK
                     AALNMHSAIVHNNIKEHGGQVILIHPGWMKSWLTGEYRDEAPLTPDIPAKKLIGIIRN
                     PERYKGERPAFIDYEGNKWEF"
     gene            2586..>3250
                     /locus_tag="PLG72_10525"
     CDS             2586..>3250
                     /locus_tag="PLG72_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ81273.1"
                     /translation="MEKRKLGFDTLQVHAGQKPDPVTGSRAVPIYQTTSYVFRNTEHG
                     ANLFALKEPGNIYTRIMNPTNDVFEQRMAALEGGVAALAVSSGSAAITYAVLNIAGTG
                     DEIVAASTLYGGTYNLFSVTLPRLGIKTVFVDPDDPENFRRAINEKTKAVFIETLGNP
                     GINIPDIEAIAAIAHENRIPLIVDNTFGTPYLIRPIEFGADVVVHSATKFIGGHGTTL
                     GGV"
BASE COUNT          821 a          833 c          814 g          782 t
ORIGIN      
        1 aaataaaatg gaataagtcg aaactgttcc tcattaccgc gttgaccgca cagttgctga
       61 tccacatatt gtatttgcgt gaaaggagaa aatgataatg aggaggaaaa atatcatcag
      121 cagagcaata actatcctcc ttgtaatact gctggcattg ttacttgccg catgcgggat
      181 cagtcagaag gatgaggttt caggcggagg tagccagaca ggttcctatg gcggcagtgg
      241 ttcagaaggg gaagcggccg gcgggatcgg caaagcgtcc ggaccgggcg ggtcaggcgt
      301 gtccgtcatc ccggacagca cagatggcgc aggtctgtac tatacggatg tgcccaaaag
      361 tgctgaaacc aacttcacca taatttataa tgaatacacg ggcagccggg ctccctgaag
      421 gatacagggc tgacctggta ccggtccttg aagacagcac gctgaagaaa agcaacgtgc
      481 tggacactta tgaatcaggg aagcattaca gccttgtgtt tgagaccatg agcggctaca
      541 aggatgtcgt cgcattctat aaagaaataa tgcaaaacgc cgagtcccgg gtggtatcgg
      601 attatgagac atgctgcgat atcaccggca ctttggacgg cgttacagta catatcggca
      661 taaacttgat gcctgagggt actctgtacc tgatagaaat ggttatttga tccggaagtt
      721 gccgggcacg tcacacacac ataagacacg gggacggtat ttacgtatcc tgcaggatcc
      781 ggaaataccg tccatgtgtc tgtgaaagcc gttcccgtgc ttaagggaaa attaaaaggc
      841 aaaaggcgcc agtcacatgg atcatcacag cattcccgct gcccagaggg cgcccctgcg
      901 gatcaggttc ctgtacatgg ggaccatgaa ggatgccagc tgatgcccgg gcgacaggta
      961 taccaccctg ccgaggccgt aattgtgcgc ccatgccgca ggccactttt tgccttccga
     1021 ttcatactcc agcaacaggg tcgtttcgag cagattgtcg aggtcgaact ggtacggctc
     1081 ttccattata tcgaagcttt ctatgccctc caggatcacg tggcctgaac ctgccggcat
     1141 gtatgtaagc actttctgat ccggatgcga cctgaacctg cctcctgcca gatgcgctgc
     1201 ctcatatcgc gcttccacca ctatcccgtt gtgtaaggcg agaaggcccc cgccgttgag
     1261 cacgtacgtc agaagcccgg cgacctgctc atcggtgagc ctgttttccg tatatgcaat
     1321 acacaggtca tacctgctga ggctgtcata cctgaaccgg tcataatcct ctgtgaactc
     1381 gacgccgaaa tcgttgagga tctccgcgag ttctttgtca ggacctttca ggggatgcca
     1441 ttccacgttt gagtaattgc cgatcattat agcctgtttg ccgcccaata tgtatctctc
     1501 ccttctcgtc agaattccca cttgttgcct tcataatcga tgaatgcggg cctttcgcct
     1561 ttatatcgct ccgggttcct gatgatcccg atgagctttt ttgcagggat atcaggagtc
     1621 agcggcgctt cgtcgcgata ttcgcctgtc agccagctct tcatccaccc gggatggatc
     1681 aggatgacct gcccgccatg ctccttaatg ttattgtgca ctattgccga atgcatattc
     1741 agcgcagcct tggaaccgca gtatgcaaac catcccttcc tgtagcatgt gcttatgctg
     1801 ccggcctcgg aggatatgtt catgataagc tttttttcgc tgttcataat cagccgtata
     1861 agggcatttg tcaccctggt ggccccgaga cagttcacat tgaagactct gagcatctcg
     1921 tcgaaatcca gttcgtcgaa tatggtggga tcatagttgc cggatatggc gctgttgttg
     1981 accagcacat cgatcctgtc cgtcctcgaa ctgatgaact ccgcagcagc tttgacgctt
     2041 gtatcgctgg acacatcgag atccacgata taaaggctgt ccggatattt ttcactcagt
     2101 ttgtcaagca acttccagtc cttgttgtag cgccccgcga acacggtgta cccgtcttca
     2161 agcagggctt ttgccagttc aaaccccagg cctctgtcgg ctcctgtcac acatgagtaa
     2221 agcttcatac tgatcacctc ttagatatta taacagatac agtgcccggc acgatacaca
     2281 gacatttaca tatacccggt tttactgaat gcagacgcag acatttacgg tcatcatatc
     2341 cggttattcc tgtgaaaccc atacatgata accgtccctg tgcttgtcag tgcctgtcgt
     2401 gcctttaccg gatttcagcg actaaaattc gttattgaca caattgaact tttcatataa
     2461 tatacaaaac atataggaaa agacggaatt aaaaaggagt aaaaaaataa tcgacgaacc
     2521 agaagatggg tatataatat tgccaaagac tcctttttac aaatatgaga tacaggaggt
     2581 cagttatgga aaaaaggaaa cttggatttg acacgctgca ggtacatgcg gggcaaaagc
     2641 cggaccctgt gaccggttcg agagcagtgc cgatatacca gaccacgtcc tatgttttca
     2701 ggaacaccga gcatggcgcc aatctgttcg cgcttaagga gcctggcaac atctacacga
     2761 ggataatgaa cccgaccaat gatgtattcg aacagagaat ggccgccctg gagggcggtg
     2821 tggctgcatt ggctgtatct tcaggttccg ccgccattac atatgctgtt ttgaacatcg
     2881 ccgggacagg agatgaaatc gtggccgcaa gcacactcta cggcggcacg tacaacctgt
     2941 tttcggtgac gctgcccagg cttgggatca aaaccgtatt cgtcgatccc gacgatccgg
     3001 agaatttcag gagagcaata aatgagaaga caaaagcggt cttcatagaa acacttggga
     3061 acccgggcat caacatacct gacatcgaag cgatcgccgc gatcgcccat gagaacagga
     3121 tacctctgat cgttgacaat actttcggga cgccgtatct catccgaccg atcgaattcg
     3181 gagccgatgt ggtggtacat tcggccacga aattcatagg aggccacggc acgaccctgg
     3241 gaggcgtcat
//
LOCUS       DAOSCC010000244         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_18945_length_3249_cov_7.213838, whole genome shotgun sequence.
ACCESSION   DAOSCC010000244 DAOSCC010000000
VERSION     DAOSCC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18945_length_3249_cov_7.213838"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..457
                     /locus_tag="PLG72_10530"
     CDS             125..457
                     /locus_tag="PLG72_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ81274.1"
                     /translation="MSEISKQLKKGVIEILILSLLSREEMYGYQLIQELDTRSSGVFK
                     MKEGTLYPVLYRLEDNRYIESFWEQGHERRSVPRKYYRITPTGLKALENMKQELDWLI
                     SAIKTILY"
     gene            528..1295
                     /locus_tag="PLG72_10535"
     CDS             528..1295
                     /locus_tag="PLG72_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ81275.1"
                     /translation="MNRINNYVNEVLSYIVADNKMKKRIREDLTSQLNEAARTQDIDS
                     VINEMGDPKEVAREFMDSIYDDKSELFDDLLSDRSDNAIYVKRVYEYRSKTSVFGIPL
                     VHIKISRYGRPALAKGIIAIGTTSLGIVSIGAIPIGIISVGGLALGVIAFGGLALGLL
                     MALGGVALGAAAFGGVVIGFGAFGGVALGKIAIGGVAAGTVAIGSEIDGEYLLKISRI
                     TPETIAEAAALIRTAFPGLPDWIVDLFTGIIKYLPGN"
     gene            complement(1483..2454)
                     /locus_tag="PLG72_10540"
     CDS             complement(1483..2454)
                     /locus_tag="PLG72_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ81276.1"
                     /translation="MKKGTSFGKFTIRRVKRKKSVKKILTIIAIVLFVIVIAGFAAIP
                     PIVMKDMIHVHADVRELPPERFGVAAEEIWLTTEDGFRLVSWEVKADNPRGIVIFLSG
                     IHNPSVTAFFGHAKMMADNGYSSLLIEMRSHGRSEGDKIYAGTREHLDTKAGVEYIKA
                     QPSYKDLPVIVFGVSMGGATAINSIGEIPDIDGLISVSAFSSWQDTFCDNMVSMGLPP
                     FIAEIQKPFVKLYMGLEYGFGSTKISPVNEIKKLNGRPALLMHSTGDSQVPYSSFERL
                     KEAAGGQVETFVREGDYHFICRDESFDKPWTDEEYAGAILSFLKEHF"
     gene            2740..2830
                     /locus_tag="PLG72_10545"
     tRNA            2740..2830
                     /locus_tag="PLG72_10545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2775..2777,aa:Ser,seq:gga)
BASE COUNT          887 a          759 c          740 g          863 t
ORIGIN      
        1 aactgggttt agcctcataa acaatttttg gatttatttt tgctcattta tagttgacac
       61 agctaatact ttccattatt atatatttag tattactaaa tacttagaaa tggagagctt
      121 gtacttgtca gagataagca aacaattgaa aaagggcgtc atagaaatac tgatactaag
      181 ccttctgagc agggaagaga tgtacgggta ccagctgata caggaattgg acacgagaag
      241 cagcggtgtt ttcaaaatga aagagggcac gctttaccct gtcctgtaca ggcttgagga
      301 caacaggtat atcgaaagct tctgggaaca gggccacgaa aggcggagcg tcccgagaaa
      361 gtactacagg ataacgccta ctggactgaa agcgctggaa aacatgaagc aggagcttga
      421 ttggctcatc tccgcgatca aaaccatctt gtactgacac gctgatgttt tactgacatt
      481 ttatataagc tttatatgaa tcttttatga aagagggtaa tgaccggatg aacagaataa
      541 acaactatgt taatgaagtc ctttcttaca ttgttgccga caataaaatg aagaaacgca
      601 taagggaaga tctgacttca cagctcaatg aagctgcccg gacacaggat attgacagtg
      661 tgatcaatga aatgggcgat ccgaaggaag tcgcccgcga attcatggat tcgatctatg
      721 atgataaaag cgaacttttt gatgatctgc tgtcagatcg ttcagataat gcgatatatg
      781 tgaagcgggt atatgaatac aggtcaaaaa cttctgtttt cgggataccg ctggtccaca
      841 tcaaaattag caggtatggc aggcctgctt tagcaaaagg catcattgcg atcggaacga
      901 catccctggg gattgtttct ataggcgcca tcccgatagg gataatcagc gtcggcggtc
      961 tggcattggg cgttatcgcg ttcggcggcc ttgccctcgg cctgctgatg gccctgggcg
     1021 gtgtggcatt aggcgccgct gcattcggcg gggtcgtcat cggctttggg gcgtttggag
     1081 gagttgccct gggcaaaata gcaataggag gagtcgctgc cggcactgtt gccataggga
     1141 gtgaaatcga cggtgaatat ttgctgaaaa taagccggat aacccctgaa accatcgctg
     1201 aagcggccgc tctcatccgg accgcattcc cggggctgcc cgattggatc gtcgatctgt
     1261 ttacaggtat aattaaatac ttgcccggca actgaattat ttccgggaaa ttgagtattt
     1321 gcatacggag agcgaaattg aatatttgct tgctgggaat gaaatagaat atttgcatac
     1381 ggagaatgaa aaataaaatg accgcagcaa cggaaaacaa attaacattt acatcaacgt
     1441 caatagctgc agccatgcgg tcatgcccga tcaggatcct attcagaagt gctctttcag
     1501 gaaagaaagg atggcgccgg catactcctc atctgtccag ggcttatcaa aactttcgtc
     1561 cctgcagatg aaatgataat cgccctcgcg gacaaaggtt tcgacctgtc cgcctgccgc
     1621 ttccttcagc ctttcaaagc tgctgtaagg cacctgcgaa tcccctgtcg aatgcatcag
     1681 gagcgcaggt ctgccgttca gcttctttat ttcattgacg ggacttatct ttgtgctgcc
     1741 gaaaccgtac tccaaaccca tgtaaagctt gacgaaaggc ttctggatct ctgcgatgaa
     1801 aggtggcaac cccatgctga ccatattgtc acagaatgta tcctgccatg atgaaaacgc
     1861 tgaaacgctt atgagcccgt caatatccgg gatctctccg atcgaattga tcgccgtggc
     1921 gccgcccatc gatactccga agactatgac cggcaggtct ttatatgacg gctgcgcctt
     1981 gatatattcg actcccgcct ttgtgtcaag gtgctccctg gtccccgcgt atatcttgtc
     2041 cccttcgctt ctcccgtggg aacgcatttc gataaggaga gaagagtatc cgttgtccgc
     2101 catcattttg gcgtggccga aaaatgcagt aactgacgga ttatgaatgc cggacaggaa
     2161 tatgacgata cccctggggt tatcggcttt gacctcccag gagacaagcc tgaacccgtc
     2221 ttcggtagtc agccatattt cttccgccgc cacaccaaat cgctccggcg gcagttctct
     2281 gacatccgcg tgcacatgga tcatatcttt catcacgatg ggcggaatgg ctgcaaagcc
     2341 ggcgataaca ataacaaaaa gaactatcgc gattatagtg agtattttct ttacagactt
     2401 cttccttttt acccgcctga tagtaaattt gccgaatgat gtaccttttt tcatcgccga
     2461 ctcctcccat cgtttcgata agatattatc actatcaaac attcacgtca acgaaatgaa
     2521 agaggctgaa cacgcattga tgtgaatgat caggttccgg gcgctgtttt cggcaataat
     2581 catggcaatg accatattgt cccgcggacc atttccgatt gacatccgtc cggttctatt
     2641 gtattatagt taagcggcac agaaagcgca tttacatgaa cctacggatg cactggatgc
     2701 ttgccgtatc aaattccata catgtattat tttcgacctg gagagttcgc ctagatggtc
     2761 gagggcgcat gactggaaat catgtatacc tgaaagggta tccagggttc gaatccctga
     2821 ctctccgcca aacccgcaaa aaccgagcgt tttgcgggtt tttagatgcc cctttttacc
     2881 tgttctgtca gagtcaggca agaattttgc tcaaagatcc cataacttct tcaggagaaa
     2941 ccaaacaaac caattcccaa attattctat cgattcgttt tttaaaaaga tccggtgaca
     3001 atccatcagt atttttaaac ttagcttcca gcaaaccgcg tacttctcca atcagccgcc
     3061 cgcgctcagg cccatgcaca ttttcaaact ttagtttttc aatacattca tcaatatctc
     3121 tgaaccgtaa aattttgttg tcattactat cccgttcatt ttacctcctt gatctggcag
     3181 atcataaatt ccagctataa atgagcaaaa ataaatccaa aaattgttta tgaggctaaa
     3241 cccagttcc
//
LOCUS       DAOSCC010000245         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_19231_length_3215_cov_3.709494, whole genome shotgun sequence.
ACCESSION   DAOSCC010000245 DAOSCC010000000
VERSION     DAOSCC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_3215_cov_3.709494"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLG72_10550"
     CDS             <1..226
                     /locus_tag="PLG72_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ81277.1"
                     /translation="GIISAFQLFNQPYILTQGGPVNATRTVAMVVFQNAFQYGRMGYA
                     SCVAWVLFVVIMAVTLIIQSSSKRWVFYDN"
     gene            259..1113
                     /locus_tag="PLG72_10555"
     CDS             259..1113
                     /locus_tag="PLG72_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ81278.1"
                     /translation="MKRSLSKSMSIKLGRALIYIILIICTVICIAPFIWMVITSFKST
                     AEAYLFPPTFIPRKWMWANYKNGWAVADFPTFTKNTIFLALTCTIGTVISSALVAYGF
                     AKFKAKMSGLLYVCLLGTMMLPAQVTLIPQYLLYYKMGLVDTYGPLLIPAWLGGGAYN
                     IFLFRQFFLSLPKELNEAATIDGAGSFQTFWRIMLPSVKPVILAVGVMSLVWNWNDFY
                     TPLIYLNSTSKFTLSIGLQFLNSSMGTTKIGQMMAVSVVTLLPVLIIFFVCQKYFVQG
                     IKMQGLKA"
     gene            1162..2633
                     /locus_tag="PLG72_10560"
                     /pseudo
     CDS             1162..2633
                     /locus_tag="PLG72_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
     gene            2869..>3215
                     /locus_tag="PLG72_10565"
     CDS             2869..>3215
                     /locus_tag="PLG72_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ81279.1"
                     /translation="MKAFKYKNPILRGYRPDPSICSVNGDYYLVNSTFEYFPGVPVFH
                     SRDLINWKQIGACLTRESQLMLRGCKPSGGIYAPTLRYHNGRFYMITTNVTGGGNFIV
                     YTDDIHGAWSEPQ"
BASE COUNT          841 a          807 c          785 g          782 t
ORIGIN      
        1 cggcatcatc tctgcgttcc agcttttcaa ccagccatat atactgacgc agggcgggcc
       61 ggtcaacgct acgcgtacgg ttgcaatggt cgtattccag aatgctttcc agtacggccg
      121 tatgggctat gcttcctgcg tggcatgggt gctgtttgtg gtaattatgg cggtgacgct
      181 gatcatccaa tcgtcatcga aaagatgggt cttttacgat aattaacgtg ataataagcg
      241 ttaggaaagg gaaatcatat gaaaagatcg ttatccaaaa gcatgtccat aaagttggga
      301 agagctttga tctatataat cctgattata tgtacagtga tatgcatcgc tccatttatc
      361 tggatggtta tcacctcgtt caagtcaacg gcggaagcat accttttccc gccgacattc
      421 atcccgagaa aatggatgtg ggcgaactat aaaaacggct gggctgtcgc cgactttccg
      481 acatttacaa aaaatactat tttccttgcc cttacctgca ccattgggac agttatttcc
      541 tcggcgctgg ttgcttacgg ttttgcgaaa ttcaaggcca aaatgtccgg acttttgtat
      601 gtatgcctgc ttggaacaat gatgctgcct gcgcaggtaa cgctgatacc ccagtacctg
      661 ctgtactata aaatggggct ggtcgacacg tacgggccgt tgctgatccc ggcctggctg
      721 ggcggcgggg cgtacaacat atttttgttc cggcagtttt tcctgtccct gccgaaggaa
      781 ctcaacgaag ccgcaacgat cgacggagca ggatccttcc agaccttctg gcgcatcatg
      841 ctgccatcgg taaaacctgt gatcctggct gtcggagtaa tgtctctggt ctggaactgg
      901 aacgacttct atacgccatt gatatatctg aattcgacca gcaaatttac cttgagcatc
      961 ggtctccagt tcctcaacag ttctatggga acgactaaaa taggccagat gatggctgtc
     1021 tccgttgtta ctctgctccc ggtgcttata atattcttcg tttgccagaa gtatttcgta
     1081 cagggcatca aaatgcaggg attgaaggct tgacgataca aagatcgaat gcttgacaaa
     1141 tgatagaaag cgaggtttct tatgctgaaa ttcacagttg aaacaaatgg taccaaaact
     1201 ccgcttgtgc attactggga aacctgcgtg ggttcctgcc atgcttacac agccctgcgc
     1261 gaagattatc gcgaacagct taccaaggct cacagggacg ccggattcaa gtatgtccgt
     1321 tccacgggct gctgaacgat gacatgagcg ttgtcgtccg caacaggaat ggcgagcttg
     1381 tatataactt cttcaatatt gattccatct tcgattttct gctcagcatc ggcatgaagc
     1441 cgttcatcga actcagctat atgcccgaag ccctggcatc gacatcggca acattgttcc
     1501 attacaaagg caacaaatca aaaccgaagg actatgccga gtgggacgcc ctgatcagga
     1561 aactgacgga acacctcgtc agccgctatg gcgcagatga agtacgcagc tggttcttcg
     1621 aagtatggaa cgagccaaac ctgctttatt tctttgaggg tacccgcgaa gactactttg
     1681 agctttacga tcacacggcc cgtgcaataa aatcggtcga cccctgcctg tccgtaggcg
     1741 gtcccgcgac ggcaataaat gcctggatac cggagatgat cgaattctgt gagaaaaacg
     1801 ggacacctct cgacttcatc tcgacccacc actatcccac ggacgattac ctgtggtcca
     1861 acggcttcgt gggagataac ctgatagaag atctcgagtc gaaaaatata gatatcgaca
     1921 aaatgcggta ttatgaacgc gggatcctga caaagatgat gaagaaggca agaacggaag
     1981 ccggcagtta tccgctctat tacaccgaat ggaacagttc cgcaatgtcg cccgatcatc
     2041 ttcatgacaa agcctatgcg gcggcattcg ttgcaaagac gataatcgac aaccttggga
     2101 tggcccaggc ctattctttc tggactttca cagacatttt cgaagaacag ggccagctcc
     2161 ccggcgagtt ccatgggggc tttggactgc aaacggtcca tgggataccg aagccgacct
     2221 acagggcttt tgaagccctt catcagctgg ggaacgaaag gatcccggtg acagaagaac
     2281 aggaaactgt gggaatgata gcatcgactg ccggaagcgg agagatctgc atcgtcgcct
     2341 acaaccacaa tatacccgac ggagagatca aaggccagca tgtcgaggtc gtgctccagg
     2401 ataaaaaagc ttctgcggcg gaaattacac ttattgatga cgaacactgc aatccggccg
     2461 ctgtttacga gcaaatgggt tctccggatt cgctcagccc gaaacagctc gacatgctgc
     2521 atgaagcttc aaaagtcacg ccagccgccc ttgcggtcga ggtaaatgac aattcttcct
     2581 gcgtcaggtt ctatctcccg ccccactcgg ttgcgcttat ccgaattatt tgatcctgca
     2641 taacggcaat ccctgaataa tggacaagta tcaaccgcct cccggctttt ttcagcatcc
     2701 aggctggaaa acgccggaag gcgtatgaca ttttgccttt cagacggcct gtatatctcc
     2761 cccgtctctt tgaaaggctg atgaaaaatt ttctgcaaat gaaaaattcc gctgaaagag
     2821 aagcattata aaagtaacac actttaatgg aaccaggaga taaacattat gaaagcattc
     2881 aagtataaaa acccgatcct ccgcggctac cgcccggatc caagcatctg ttccgtaaac
     2941 ggagattatt acctggtcaa ctcgaccttt gagtattttc cgggcgtccc ggtcttccac
     3001 agtagggatc tgatcaactg gaaacagatc ggagcctgcc tgacgcgtga aagccagctg
     3061 atgcttcgcg gctgcaagcc ttcgggaggt atctatgcgc cgactctcag gtatcataac
     3121 ggcagatttt acatgataac gaccaatgtc acaggcggcg ggaactttat cgtttacacc
     3181 gacgatatac atggtgcttg gtcggagccg cagtg
//
LOCUS       DAOSCC010000246         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_19455_length_3188_cov_4.211299, whole genome shotgun sequence.
ACCESSION   DAOSCC010000246 DAOSCC010000000
VERSION     DAOSCC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19455_length_3188_cov_4.211299"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLG72_10570"
     CDS             <1..200
                     /locus_tag="PLG72_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81280.1"
                     /translation="AYDFIIRTILSGRWKDGLQVDYFHLQPLIFADRTLHDLKATRTP
                     HDLYDREKPHRRAGSEILRTI"
     gene            216..1481
                     /gene="hflX"
                     /locus_tag="PLG72_10575"
     CDS             216..1481
                     /gene="hflX"
                     /locus_tag="PLG72_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ81281.1"
                     /translation="MELKERALLTGVNLNNDDDFEKSMDELENLAEACGFEVAGRVEQ
                     NLRSVSSAFYFGAGKVEETAELVEAMEADVVIFDNELTPTQQRNLEKALGCRILDRTQ
                     LILEIFAQRARTREARLQVEAARLQYMLPRLAGKGVSMDRQGGGGIGTVVRGGGEKKI
                     VLDRRRIEQNIAVLRKELEDLRKDREIQRKRRSESGLPRVALVGYTNAGKSTVMNALV
                     ELSQKPDSKKVFEKDMLFATLETSVRSIELPDSRSFLLSDTVGFVSKLPHELVKAFRS
                     TLEEVKEADLLLHVVDASDPEAERQIRVTVDTLEQIGAGDIPVIYVFNKADLAGMAYP
                     FEKDNAIYISAKERIGIRELAELICRYVFRTYIDCMMLIPYEYGNIVSYFRENALVKD
                     IKYESDGTWLQLECSESDYRRYRQFVTEK"
     gene            1631..3115
                     /locus_tag="PLG72_10580"
     CDS             1631..3115
                     /locus_tag="PLG72_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOJ81282.1"
                     /translation="MNRLGFDNGKYLRLQSQKILERIEQFGGKLYLEFGGKLFDDYHA
                     SRVLPGFKPDSKVKMLMQLKDQAEIVIAINADDIEKSKRRGDLGITYDLDVLRLIDAF
                     REIGLYVGSVVITHYRSQHTADIFQKRLMNLGMKVYRHYPIPDYPSNIPLIVSDEGFG
                     KNEYIETDKSLVVVTAPGPGSGKMAVCLSQIYHENKRGVKAGYAKFETFPIWNLPVKH
                     PVNIAYEAATTDLNDVNMIDPFHLEAYGVTAVNYNRDIEIFPVLKAILEKIEGTSPYK
                     SPTDMGVNMAGLCITDDEAVCEASKQEVIRRYFNTRCAHRQGLADRAEVYRLELLMKQ
                     LGISEADRPVVQAAKQRAESTGGPAVAIQLHDGRIVTGKTSPLLGASAAMLLNALKEL
                     GGIHHDIHLISPIVIEPIQALKVNHMGNHNPRLHTDEILIALSICAATNPTAALALEQ
                     LPKLRGCEAHSSVILSRVDENVFQKLGVNITCEPLYQTKKLYHK"
BASE COUNT          897 a          745 c          895 g          651 t
ORIGIN      
        1 atgcatatga tttcatcatc aggacaatat tatccggtag atggaaagat gggctgcagg
       61 tggattattt tcatttgcag cctttaattt ttgccgacag gacactgcat gacctgaagg
      121 ccacacggac tccgcatgat ctgtacgaca gggaaaagcc gcatcgccgg gcaggatcag
      181 aaatactgcg tacaatctga ttagggagtg gttatatgga acttaaggaa agagcgctgc
      241 ttacaggcgt aaatctgaat aatgacgatg atttcgaaaa atcgatggat gaactggaaa
      301 acctggctga ggcctgcggc tttgaagtgg ccggaagggt cgaacagaat ctcagatccg
      361 tcagcagcgc cttttacttc ggcgcaggca aggtggagga gactgcggaa ctggtcgaag
      421 caatggaggc tgatgtcgtc atattcgata atgaactgac gccgacccag cagaggaacc
      481 ttgaaaaagc acttggctgc aggatactgg acaggacgca gctgatactt gagatatttg
      541 cgcaaagggc aaggacaagg gaagccaggc tgcaggtcga agccgcaagg ctgcagtaca
      601 tgctccccag gctggctgga aagggcgtat ccatggacag gcagggcggc ggcggtatcg
      661 gcactgtcgt caggggcggc ggtgagaaaa agatagttct cgaccgcagg aggatcgaac
      721 agaatatagc cgtccttagg aaagaactcg aagatctcag gaaagaccgg gagatccagc
      781 gcaaaaggag aagcgaatcc ggactgccgc gggtcgcact ggtgggctat accaacgcgg
      841 gcaaatcgac ggtcatgaac gccctggtgg agctttcaca gaaacccgac agcaagaagg
      901 tattcgaaaa agacatgctt ttcgcaacgc tcgagacttc ggtccggagc atagaactcc
      961 cggacagcag gtctttcctg ctgtcggata ccgtagggtt cgtcagcaaa ctaccccatg
     1021 aacttgtcaa ggcattccgt tcgactctcg aagaggtgaa ggaggcggac ctgctgctgc
     1081 atgttgtgga tgcttcggat cccgaagcgg aacgacagat cagggtgacc gtggatacgc
     1141 tggagcagat aggcgccgga gacataccgg tgatctatgt gttcaacaag gcggacctgg
     1201 ccggcatggc ctatcctttt gaaaaagata acgctatcta tatttcagca aaggaaagga
     1261 tcggcatcag ggaacttgcg gaacttatct gcaggtatgt tttcaggact tatatcgact
     1321 gcatgatgct gataccgtat gaatacggga acatcgtttc gtacttcagg gaaaacgcgc
     1381 tcgtgaagga cataaagtat gaaagcgacg ggacctggct gcagctggaa tgcagcgaga
     1441 gcgattacag gcgctacagg cagtttgtga cagaaaaata acgaatagac tttaaaaacc
     1501 gaaaaaaatc caaacaggga ccggttgtcc gattgacgca ggttggctga tgtgttattg
     1561 tgggtaagga aaaatatcgg caggaaaatt ttcgacaaaa ctgcttacta aatgatacag
     1621 gagtataaaa atgaacaggt tgggttttga caacggcaag tatctgcgtc tgcagtcaca
     1681 gaagatccta gagcgtatcg agcagttcgg cgggaagctg tatcttgaat ttggcgggaa
     1741 acttttcgac gattaccatg cctcaagggt gcttcccggg ttcaaaccgg acagcaaggt
     1801 caaaatgctg atgcagctga aggatcaggc cgagatcgtt atagcgataa atgccgatga
     1861 catagaaaaa agcaaaagaa gaggcgatct tggcattaca tacgacctcg acgtacttcg
     1921 cctgatcgat gcattccgcg agatagggct gtacgtcgga agcgtggtca taacccatta
     1981 ccgttcacag cacactgccg atatattcca gaagaggctc atgaacctcg ggatgaaagt
     2041 atacaggcac tacccgatac ctgactaccc ttccaacata ccgctgatcg tcagtgatga
     2101 gggtttcggg aaaaatgagt atatagaaac ggacaagtcc ctggtcgtgg tcacggcacc
     2161 ggggccgggc agcgggaaaa tggccgtctg cctgtcgcag atatatcacg agaataagag
     2221 gggcgtcaag gcgggatatg ccaagttcga gacattcccc atctggaacc tccctgtcaa
     2281 acaccctgtt aatatagcgt atgaggctgc aacgacggac ctgaacgatg tgaacatgat
     2341 agacccgttc cacctggagg cttatggcgt gaccgccgtg aattacaaca gggatataga
     2401 gattttcccc gttctgaagg ccatcctgga aaaaatagaa gggacttcgc catacaagag
     2461 cccgaccgac atgggcgtca acatggccgg gttatgcata acggatgatg aagcggtctg
     2521 cgaagcttcc aagcaggaag tcatacgaag gtatttcaat acccggtgtg cacatcgtca
     2581 ggggctggct gacagagcgg aagtatacag actggaactg ctgatgaaac agttggggat
     2641 ctccgaggct gaccgtccgg tggtacaggc cgcaaaacag cgcgctgaat ccacaggcgg
     2701 accggctgta gccatacaac tccatgacgg caggatcgta accggaaaga cttccccgct
     2761 cctgggcgct tcggctgcga tgctgctcaa tgcactgaaa gaacttggag gcatccacca
     2821 cgacatacat cttatctcgc cgatagtcat agagccgata caggccctga aggtcaatca
     2881 catgggtaat cataacccgc gcctgcatac cgacgagata ctgatagcat tgtccatatg
     2941 tgccgctacg aacccgactg cggctttggc actggagcag ctgccgaaac tgaggggatg
     3001 cgaagcccac tcatcggtca tattgtcgcg cgtcgatgag aacgtgttcc agaagcttgg
     3061 cgtcaatatc acatgtgaac cgctgtacca gactaagaag ctttatcata aatagaatga
     3121 ctgaacacga tcgaaccgat tgaagcttat cacattgaag ccctgtcacc tgcccagtat
     3181 cctgccca
//
LOCUS       DAOSCC010000247         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_19532_length_3179_cov_4.430858, whole genome shotgun sequence.
ACCESSION   DAOSCC010000247 DAOSCC010000000
VERSION     DAOSCC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19532_length_3179_cov_4.430858"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2584)
                     /locus_tag="PLG72_10585"
     CDS             complement(<1..2584)
                     /locus_tag="PLG72_10585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ81283.1"
                     /translation="MSRNRKIILHTVAFVLIVAAITFGITFVSKLNNELASKITSSIF
                     DEKTFSLSGWQYRWGDSPVDAGGRFLWMDKDYDYTGWTDYEFPGRPSNRGNYRSIWIR
                     AVLPDIDMENASLRLRTPQNTIEVYLEGRLIYRYGTHNPSYSARTPGSIWHFVDLPDG
                     FGGKTIYIRLSTPFPHLAGYMSQAAVGLRSTHYLEALESNLSMLLIGSLCIFLGAAII
                     MVQVLSLYRKGSDIYLGLGSVFLGGWMLMECNLFQLLAPVPVSMMYMANFFIYLAPMW
                     FLIYIERKYIIQNTHYARLMKIQALLHAILTVVAFALDFSGLVSLLYSVRVFTVLFAL
                     SVVVCVYATIRSIIDGKRSARIVIIGILALGISGLLDIFSIFYNTSEQNSFDTVSQVG
                     MLIFLTVLMINAALELRKLYRQMEQQSKETETNYKSLFTNMTDGFTLNRLVYDDGGHL
                     EHCIICEANDAFSEKIGISRDALIGSDLLQLLPEIAEMCPERSDFYDTAEETAAAREA
                     HITDEAVRLKGNWYKLSIFYPQKDYMSIIFSDVTAMKNAEETIRRQAYTDSMTGFCNR
                     LSFESEMSRMNTELDTLKPLSIVVIDIDGLKITNDTFGHNAGDELLKKAASIIRKVFR
                     NRGSISRIGGDEFCVLMPYTDLKTAQEKIEHLIKLQNRLNCIYSDIPISMSIGIASTE
                     ETEGNEDIYSVYRRADDDMYRYKISQTSSEKSRVVDMLIAALSERDFVAQGHVERISE
                     LCMKMADALELNESQKRNLLLLSKIHDLGKIGVPDEILNKPGKLTKKEYEKMKTHVNI
                     GYNIASRAKELVSVAPFILHHHEHWNGAGYPDGLREEDIPLECRILSIVDAFDAMTND
                     RPYHK"
     gene            2710..>3179
                     /locus_tag="PLG72_10590"
     CDS             2710..>3179
                     /locus_tag="PLG72_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ81284.1"
                     /translation="MRKSFASGAVILTAAGLAARMLGFVYRVYLSNLIGAEGMGLYEL
                     VIPVYTAVVLTITSGITIAVSKMVAEQNARSNPANTGRVTVCAIWLAAGAGMLVSFVI
                     SLNAGVFAEKVLGDGRTLGALMILAPCLPGVVAASALKGYFYGMRRVVPTAVSQ"
BASE COUNT          766 a          791 c          782 g          840 t
ORIGIN      
        1 ccttgtgata tggcctgtca ttggtcattg cgtcaaacgc gtcgactatt gagaggatcc
       61 tgcattccag cgggatatcc tcctccctga gcccgtcagg atagcccgcg ccgttccagt
      121 gttcatgatg gtgcaggatg aaaggcgcca ccgaaaccag ttccttggcc cggcttgcta
      181 tattgtagcc gatgttgaca tgggtcttca tcttctcata ttctttcttc gtcagtttcc
      241 cgggcttgtt cagtatttcg tcaggtacgc ctatttttcc gaggtcgtgt atttttgaca
      301 gaagcagcag gtttctcttc tggctttcat taagttccaa tgcatcagcc attttcatgc
      361 agagctcgga tatcctctcc acatgcccct gggcgacgaa atccctctcg gaaagagcgg
      421 cgatcagcat gtcaacgacc cggctttttt cgctgctggt ctgactgatc ttgtacctgt
      481 acatatcatc gtccgccctg cggtaaacgc tgtagatatc ctcgtttcct tcggtttctt
      541 ccgtggacgc aatccctatc gacatgctta tcgggatgtc ggaatagatg cagttcagcc
      601 tgttctgcag ttttatgaga tgctcgattt tttcctgtgc ggttttgaga tccgtgtaag
      661 gcatcagcac gcagaactcg tctccgccta tccttgagat cgaaccgcgg ttcctgaaca
      721 ccttccttat gatcgacgcg gcttttttga gcagctcgtc gcctgcattg tggccaaagg
      781 tatcgttggt tatcttcagg ccgtcaatat cgatcacgac aatgcttaaa ggtttgagcg
      841 tgtccaactc ggtattcatc cgtgacatct cggattcaaa ggacagcctg ttgcagaaac
      901 ctgtcatact gtccgtataa gcctgccgtc ttatggtctc ttcggcgttt ttcatcgctg
      961 ttacgtcgga gaaaatgatg ctcatataat ctttttgagg gtagaagatc gaaagtttgt
     1021 accagtttcc cttcagcctg acggcttcgt cagttatatg agcttctctc gcggccgccg
     1081 tttcttcggc ggtatcgtaa aaatcagagc gttccgggca catctctgcg atttccggca
     1141 acagctgcag cagatcggaa cctataagcg catccctgct gatcccgatt ttttccgaga
     1201 atgcatcgtt tgcttcacaa attatacagt gttccaggtg cccgccatca tcgtatacga
     1261 gcctgttcaa agtaaatccg tcagtcatgt tggtaaacag cgatttgtag ttggtttccg
     1321 tttcctttga ctgctgttcc atctgcctgt aaagctttct aagttccagc gccgcattta
     1381 tcataagtac ggtgaggaaa atgagcatgc cgacctggga aacggtgtcg aagctgttct
     1441 gctcagacgt attgtagaat atggaaaata tatccaacaa gcccgagata ccgagggcaa
     1501 gtataccgat gatcactatc cgggcgcttc tttttccgtc aatgatcgat ctgatagtcg
     1561 catatacgca aaccactacc gataaagcaa acagtacagt gaacactctg acagaataaa
     1621 gaagtgagac cagacctgaa aaatcgaggg caaatgcaac gaccgtgagt attgcgtgca
     1681 gcagtgcctg tatcttcata agccgcgcat aatgagtgtt ttgtattatg tatttgcgtt
     1741 ctatgtatat caggaaccac atcggcgcca ggtaaataaa aaagttggcc atgtacatca
     1801 tcgaaacggg tacgggcgcc agcaactgga acaggttgca ttccatgagc atccatcctc
     1861 cgaggaagac agagccaagc ccgagatata tgtcgctgcc cttcctgtaa agggacagga
     1921 cctggaccat gatgatggct gccccgagga atatgcacaa actgccgatc agcagcatcg
     1981 acaaattcga ttccaaagct tccaggtagt gtgtcgacct gagaccgacc gcagcctgtg
     2041 acatataacc ggccaaatga gggaaaggtg tactcaaacg tatgtagatg gttttacctc
     2101 cgaagccatc cggcagatcg acaaagtgcc atatgcttcc gggcgtcctt gcactgtatg
     2161 aggggttatg ggtaccataa cggtatataa gcctgccttc aagatatacc tcgattgtat
     2221 tctggggagt ccttaatctg agggaagcgt tctccatatc gatatcagga aggacagccc
     2281 ttatccatat cgagcggtaa ttgcccctgt tggaaggcct ccccgggaat tcgtagtctg
     2341 tccagcctgt ataatcataa tccttgtcca tccaaaggaa ccggccgccg gcatccaccg
     2401 gcgaatcacc ccaacggtac tgccagccgc tcagactgaa tgttttttca tcaaaaatgg
     2461 aggatgtgat tttcgatgca agctcattat tgagttttga cacaaaagta ataccgaatg
     2521 taatcgctgc cacgatcaaa acgaaagcaa ccgtatgtag tattattttt ctgttgcgag
     2581 acattctatc accacgtata catccgtata agcactatcc attataatac cataattcta
     2641 agagaaataa agcatataaa aataaacata ataatatgaa tgagacaggg gtcaatgtgg
     2701 ggtgaggccg tgagaaaatc attcgcaagc ggcgccgtga tcctgacggc tgccgggctg
     2761 gcggccagga tgctcggctt cgtatacagg gtgtatttgt caaacctgat aggcgcggaa
     2821 gggatgggct tatatgagct tgtcataccc gtctacactg cggtagtgct gaccataacc
     2881 tcaggcataa ccatcgcggt atcgaaaatg gttgcagagc agaatgccag gtcgaatccg
     2941 gccaataccg gaagggtaac ggtgtgcgca atctggcttg cggccggggc cggcatgctg
     3001 gtttctttcg tgatctcact gaatgccggc gtcttcgcag aaaaagtgct tggcgacggg
     3061 cggacgctcg gagcgctgat gatactggcg ccgtgccttc ccggcgtcgt tgcggcgtcg
     3121 gcgctgaaag gatacttcta tgggatgcgc cgggtcgtac ccaccgctgt ttcacagat
//
LOCUS       DAOSCC010000248         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_19745_length_3154_cov_3.829945, whole genome shotgun sequence.
ACCESSION   DAOSCC010000248 DAOSCC010000000
VERSION     DAOSCC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19745_length_3154_cov_3.829945"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2461
                     /locus_tag="PLG72_10595"
     CDS             71..2461
                     /locus_tag="PLG72_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81285.1"
                     /translation="MKKLTTIVLAVLILCLQVFSCTVAYGANGDGNAIYIKSYTAGRA
                     SVNKDESVTIGLVLENTSDNELTDIKIEVDGSSSFSSVYSGERKVLDKIAAKDTEPAA
                     INVVYKGYGNELKINIKYNDGSGEKTQAGRIIVTEVVPVQEPPTDTSQYVPRLVVDAG
                     QMPVINAGSTYKLKYRIENKSGHQAKNIDLSLNLMDESKTPLVFENYYDMDRYISGIN
                     GNDSEEVVFDVKIGNLAPEGIHPLKLRMQYENAFGNQFTSEETVYIRVANNNRSPIIT
                     VESINVKKSGAASDVITLELKLKNLGNLRADDVKVTLGGLRTDGYSLHNTTDVRYIDL
                     IYGNGSATVSYDLLPPASGAAGSNELTVSFEYKDSAGSKYNEQYKIFVPAEEGEGARP
                     NLAFSQISAPRGALTAGQEFNVSFDLKNNGGASARNVKVTLNTETGIVVRSMNPVYFD
                     RIEGNKSQKVTFTLYTADDAATKNYPISMNVEYEDAFGNKFTATQYIGVFVENDAGKT
                     VPRIIIDNYTIDPFPVSAGGEFSLKMSFLNTNKTVDVSNIKVTVTSEDGTFTPTESGN
                     TFYIENIPRTQNVERELVLQAKPDAEQKSYMLTVNFEYEDEKGNPYTAKESISVRVLQ
                     SPRLVTGDISMMTETFVGQPVSVYLDFYNMGKSTLYNLMVSVEGDFQGQGLSYYVGNF
                     EPGRTDYFDASFTPMNPGMQKGAILFTFEDANGKTTEIRKEFELNVMEMAMPEPSFES
                     PDNIPGMEMPGMEKGSSKVSIWIYIGIGAAVVIAAVVVVIVLRKRAVRRKELSLDE"
     gene            2454..>3154
                     /locus_tag="PLG72_10600"
     CDS             2454..>3154
                     /locus_tag="PLG72_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ81286.1"
                     /translation="MSKRDIIRLAFRNFMRRKTRSVLTILGVLIGTAAIVVMVSLGIG
                     MNESFRRQLSKMGSLNVIEVNRWYFEEDGKGGGYSRESVLDDNAVAKFEAIEGVEAVM
                     PIFQTSLKLVSGKYMMYANIMGIDPKIQEAFDFKVAKGRLLTEEDTTALLFGSDTPYQ
                     FYNPRSRNNFEGYYYFGGSVDGERPEPDVDVLNDKMVMTFDYSYGERIPGGGGQTGGR
                     QPKLYKVSGVGILEPS"
BASE COUNT          937 a          699 c          871 g          647 t
ORIGIN      
        1 atcaagaggc tttcgcggca taaaagctta tgcagtctgt tgggataaaa taaatccagg
       61 ggggtttcaa atgaagaagc tgacaacaat agtattagca gtactgatat tgtgcctgca
      121 ggtgtttagc tgcaccgttg cgtatggcgc caatggtgat ggaaatgcca tatatatcaa
      181 gagttatacg gcaggcaggg catctgtcaa taaagatgag tctgttacca ttggtttggt
      241 actggaaaat acatcagaca atgaattgac tgatataaag atagaagtgg acgggtcatc
      301 ttctttcagc agtgtttata gcggagaaag aaaagttttg gataagattg cggcaaaaga
      361 tactgaacca gcagctataa acgtggtcta caagggctat gggaacgagt tgaaaataaa
      421 tataaaatat aatgacggca gtggagaaaa aacgcaggca ggcaggatca tagtaaccga
      481 agttgtcccg gtccaggaac ctcccacaga tacgagccag tatgttccca ggctggtggt
      541 cgacgccggg cagatgccag tgataaacgc aggcagtaca tacaagctga agtacaggat
      601 cgaaaacaag agcggacacc aggctaaaaa catagacctg tcgctgaacc ttatggacga
      661 atcgaaaacc ccgctggtgt ttgaaaacta ctatgacatg gaccggtaca tttccggcat
      721 aaacgggaat gactcggaag aagtagtatt cgatgtcaag atcggcaacc tggctcccga
      781 aggtatacat ccgctcaagc tgaggatgca gtatgaaaat gcgttcggca accagtttac
      841 atccgaagag accgtatata tcagggtggc gaacaacaac aggagtccca taatcacggt
      901 cgagagcata aacgtgaaaa agtcaggtgc cgcttcggac gtgataaccc ttgaactgaa
      961 gctgaaaaat ctcggaaacc tcagggccga tgacgtaaaa gttacgctcg gaggactgag
     1021 gactgacgga tacagcctcc acaacaccac agatgtcagg tatatcgacc tgatatacgg
     1081 aaacggttcg gcgaccgtat catatgacct gctgccgcct gcgtccggag cagcaggcag
     1141 caatgaactg accgtatcgt tcgagtacaa ggattcagcc ggcagtaaat acaatgaaca
     1201 gtacaaaata ttcgtccctg cggaggaagg cgaaggcgca agacctaacc tggcattcag
     1261 ccagatatcg gcgcctcgtg gggctctcac agcagggcag gaattcaatg tgagcttcga
     1321 tctgaagaac aacggcggag cgtcagccag gaatgtaaaa gtcactttga acacagaaac
     1381 gggcatcgtg gtcagatcca tgaacccggt ctatttcgac aggatcgaag gcaacaagtc
     1441 gcagaaagtc acgttcacgt tgtatactgc cgatgacgct gcaacgaaga actacccgat
     1501 atcgatgaac gtggaatacg aggacgcctt cggaaataaa ttcaccgcga cgcagtatat
     1561 aggcgtgttc gtggaaaatg acgcgggcaa gacggtcccg aggatcatca tagacaatta
     1621 caccatagat cctttcccgg tgagcgccgg cggcgaattc agcctgaaaa tgtcgttcct
     1681 caacaccaac aagactgttg acgtatccaa cataaaggta acggtcacat ctgaagatgg
     1741 tacctttacc ccgactgaat cgggcaatac gttctatata gaaaacatac cacggacgca
     1801 gaacgtcgaa cgtgaactgg tgctccaggc aaaacctgac gcggagcaga agtcatatat
     1861 gctgacagtc aattttgaat atgaggatga aaaagggaac ccgtacaccg caaaggaatc
     1921 cataagcgta agggtgctcc agagcccgag gctcgtgaca ggcgacatca gcatgatgac
     1981 tgaaacattc gtaggacagc ctgtatcagt ctacctggat ttctacaata tgggcaaatc
     2041 cacgctctac aaccttatgg tcagtgtcga gggggatttc cagggacagg gcctgagcta
     2101 ttatgtaggc aacttcgaac cgggcaggac cgattacttc gacgcttctt tcaccccgat
     2161 gaacccgggg atgcagaagg gcgcgatact gtttactttt gaagatgcaa acgggaaaac
     2221 cacagagata cggaaagaat tcgaactgaa tgtcatggaa atggcgatgc cggagccttc
     2281 gttcgaaagt ccggacaaca taccgggtat ggaaatgccg ggcatggaaa aaggatcttc
     2341 gaaagtgagc atatggatat acattggtat cggtgcagcg gtcgtcatag cggcagtcgt
     2401 ggttgtgatc gtcctcagga agagggctgt aagaagaaag gaactgagtc tcgatgagta
     2461 aaagggacat tataaggctt gctttcagaa acttcatgag aaggaagacc aggtccgtcc
     2521 tgacgatact cggcgttctc ataggtacgg ccgccatagt tgtcatggtg tcgctcggca
     2581 tagggatgaa tgagagcttc aggcggcagc tcagcaagat gggcagcctc aatgtcatcg
     2641 aggtgaaccg ctggtatttt gaagaggacg gcaaaggcgg cggctattcg agggagagtg
     2701 tgctcgatga caatgccgtg gcgaagtttg aggccattga aggcgtggag gccgtcatgc
     2761 cgatattcca gacgtcgctg aaactggttt ccggcaaata catgatgtac gcaaatataa
     2821 tgggcatcga ccccaagata caggaggcct ttgatttcaa agttgcaaag ggaaggctgc
     2881 ttaccgagga ggatacgacg gcgctcctct tcggctccga tactccttac cagttttaca
     2941 acccgcggtc caggaacaac ttcgaaggat attattactt tggcgggagc gtggacggtg
     3001 aaaggcctga gcctgatgtg gacgtcctga acgacaagat ggtgatgacc ttcgattaca
     3061 gctacggaga gcggataccc ggcgggggcg gccagacagg cggcaggcag ccaaaactgt
     3121 acaaagtcag cggcgtcggt atactggaac cttc
//
LOCUS       DAOSCC010000249         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_19757_length_3153_cov_3.407360, whole genome shotgun sequence.
ACCESSION   DAOSCC010000249 DAOSCC010000000
VERSION     DAOSCC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19757_length_3153_cov_3.407360"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLG72_10605"
     CDS             complement(<1..500)
                     /locus_tag="PLG72_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81287.1"
                     /translation="MYSDFMNRRKMRTVLQFGCIFLVLLIMLSACSRGKRADNAGNGH
                     VPAEPAEDSQPSVGETEENGTGHTETGDMAEDRSDKTAEHDNVSGNGGAEISVGPELD
                     DFLMLDEFFKNHDFDVSYDEKQEFHERTYMIRGTYDMNGDGEEDEITLLIKTDYSEGS
                     YIEVNG"
     gene            complement(669..1796)
                     /locus_tag="PLG72_10610"
     CDS             complement(669..1796)
                     /locus_tag="PLG72_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOJ81288.1"
                     /translation="MKTIVKFVKKEAVLCISLLLALISMAFVPPDKEYIRYIDFHTLA
                     VLLCLMVIMAGLRSLGIFTVIGGWLLGKTHSVRSLAMVMVLLCFGFSMVITNDVALIV
                     FVPFAIDVLVMAGLEDCLVRVIVLQTIAANLGSMLTPIGNPQNLYLYSLSGMGLGSFI
                     TLMLPYTLLSFIGLLAACAILFRGKGIDVKVWSGHTLAASEKRETAVYLLLFLAAVAS
                     VARIISVYAAGAVVLAVIFLMDRRMLLKADYALLLTFVFLFVFIGNMKRIPEISLWLQ
                     SAIDGREMIVSVAASQVISNVPAAILLSGFTDNLPALIAGTNLGGLGTIIASMASLIS
                     FKFYGTTADRRNTAYLGVFTLLNLVFLAALLLLWHVIQWCR"
     gene            2158..2409
                     /locus_tag="PLG72_10615"
     CDS             2158..2409
                     /locus_tag="PLG72_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81289.1"
                     /translation="MVEDVSVVEDASVVEDMSVVDASVVEDMSVVEDDSDESVSVEEE
                     FVVVVPELFPPQPAIVTTITTAIISARSLLIFIILPPFY"
     gene            complement(2645..2971)
                     /locus_tag="PLG72_10620"
                     /pseudo
     CDS             complement(2645..2971)
                     /locus_tag="PLG72_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009533975.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
BASE COUNT          862 a          804 c          675 g          812 t
ORIGIN      
        1 tttccattga cttcgatata tgatccctcc gagtagtcgg tttttatcag gagagtaatc
       61 tcatcttcct ctccatctcc attcatgtca tatgtcccac ggatcatata ggttctctca
      121 tggaactcct gtttttcatc atagctgaca tcgaaatcat gatttttgaa aaactcatcc
      181 agcatcaaaa agtcatcaag ttcagggccc accgaaattt ccgcgccgcc attgccggac
      241 acgttatcgt gctccgctgt cttatctgat cgatcctcag ccatatcacc cgtttctgta
      301 tgtccggtac cgttctcttc agtctccccg acagacggct gtgagtcctc tgccggttct
      361 gccggtacat gcccgttgcc ggcattgtct gctctttttc cgcgagaaca tgcggaaagc
      421 atgatcagca aaaccaaaaa aatgcagcca aactgcaaga cggttctcat tttcctcctg
      481 ttcatgaaat cgctatacac atctgttcct ccatcagtta gttacattga tcatcacaga
      541 tataaatcaa ataatatgaa cacaacattt gtcatatttt actaatatta tattgttacc
      601 gaccaactaa agtcaacaaa ctggaatcca atatatactg acgtaaaaat cccatgtttg
      661 ttcttccgtc atcggcacca ttggatcaca tgccacaaaa gcagcaacgc ggccaggaaa
      721 accaggttga gcagcgtgaa tacgccaagg taggccgtat tccgcctgtc cgcggtcgtg
      781 ccgtaaaatt tgaatgaaat aaggcttgcc atcgaagcaa tgatcgttcc aaggccgccc
      841 agattggtgc ctgcgatcag tgccggcaga ttatcggtaa agccggaaag aagaatagcc
      901 gcaggaacgt tgctgataac ctggctggca gccactgata cgatcatttc gcgcccgtct
      961 atagcgctct gcagccagag gctgatttcc gggatgcgct tcatattgcc gataaaaaca
     1021 aataagaata cgaaagtaag cagcagggca taatcagctt tcagcagcat ccgccgatcc
     1081 atcaggaata ttaccgccag gaccactgct cccgcggcat atacggaaat tatacgggca
     1141 acgcttgcca ctgcagccag gaacagcagc aggtaaacgg cagtttcccg tttttcagac
     1201 gcagcaagcg tatgtcctga ccacaccttc acgtcaatgc ccttcccccg gaacagtatt
     1261 gcacaggcag ccagaagtcc gataaatgaa aggagggtat aaggcagcat cagggtaata
     1321 aagctgccca ggcccatacc gcttaatgag tacaggtaca agttctgagg attgcctatc
     1381 ggcgtaagca tactgcccag gttggcggca atggtctgca gaacgataac ccggaccagg
     1441 caatcctcaa ggccggccat cacaagtaca tcgatggcga acggcacaaa aacaatgagg
     1501 gcgacatcat tggtaatgac catgctgaac ccgaaacaca gcagcaccat taccatcgcc
     1561 aggctccgca cactgtgcgt ctttcccagc agccagcctc caatgaccgt aaatatgccc
     1621 aggctccgca gccctgccat aatgaccatc aggcagagca ggacagccaa cgtgtgaaag
     1681 tctatgtatc tgatatattc tttatccggc ggcacgaatg ccatggatat gagagccagc
     1741 agcaaagata tgcacaacac cgcttctttt tttacgaatt tcacgatcgt tttcatcccg
     1801 tccttacccc aatcaactga aattatgtcg caaaattgca aacaaatcca taggcaaacc
     1861 catgtaactg ccattgacat ttttcattat catatatcct caccaagtct accacagagt
     1921 ttttaggaat gtaagtgctt atcataactt ctctatcatg gatgaaggaa tgggagccgt
     1981 ctccggaact gccgagcagt tcggggaatt ggtaaagctc agataaacat tgctgatgat
     2041 ctgtatatat gagattaaga aggaaatgcc tcttcaatga ctgaagaggc atttccaaac
     2101 agattattaa aggctaaaaa cagcatattt gattatccgg ccaaagaaga cgtatctgtg
     2161 gtcgaagacg tatccgtcgt cgaagacgcg tctgtcgtcg aagacatgtc cgtcgtagac
     2221 gcatctgtcg ttgaagacat gtccgtcgtc gaagacgact ctgacgaatc agtctctgtc
     2281 gaggaagaat tcgtcgttgt cgttcccgag ttattcccgc cacagcctgc aatagtgact
     2341 accatcacaa ctgcgatcat tagtgccaga agccttttga ttttcatcat tctccctccc
     2401 ttttattaaa gtttcttctt gattatataa tcatagtgca ttttttcaag cgagctgttt
     2461 gcccaaatac gtgaaaaaaa caggacttag gccaatcatc ccaaaactaa gctggatata
     2521 tctcaccatg atttcatctt tcatcggttt gacatagttg tataataggg gagagggagt
     2581 atttgtattt gaggagagaa ggccataaat ccgcttctgg aagcgttgat gctgccatat
     2641 aatgttatct tatgaaatgc ttcgcaaccc tctcctctgt ttccggcagt ccgaattcct
     2701 ttttaccgag ttcgatgctt ttcataagga aagccatatt cctggccagg gtcctcatgg
     2761 tctgcatccc ctcctcgtcc tgttcggcct cgccgggaag aaggccatgt atcgaattcc
     2821 agtactggct ggacgcaacc ggcattcctg acagcgtgaa gtatttgttc agctgatcga
     2881 aagtcgatga acatccgcct ctcctggcac atacgacgct tgccccgacc ttcatggtct
     2941 tgtcaaatgg ggtactgtaa aatagcctgt cactgactga gcccggcttt cctgcggctt
     3001 cattattcct tcctttagca gtataatatt aagtgggcag gattcggcag caattccgga
     3061 atatatggca tcaattctga tcgccggcaa gatgctgtaa aatacgagat cttataaatc
     3121 aaagggacga atatgacagg tatggtcgtt gtc
//
LOCUS       DAOSCC010000250         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20000_length_3124_cov_3.855002, whole genome shotgun sequence.
ACCESSION   DAOSCC010000250 DAOSCC010000000
VERSION     DAOSCC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20000_length_3124_cov_3.855002"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..128
                     /locus_tag="PLG72_10625"
     CDS             9..128
                     /locus_tag="PLG72_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOJ81290.1"
                     /translation="MPAEVKPEEAKAEYRDGILTVTVPKSEPSKSKGRKIDIQ"
     gene            465..1508
                     /locus_tag="PLG72_10630"
     CDS             465..1508
                     /locus_tag="PLG72_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ81291.1"
                     /translation="MITQKEIARMCGVSTSTISNVLNGKRKVSEATRQKVLEIVKKTG
                     YQPNYFAQGMRKQKTNIIGIIAEDLIQFSSPPIIERIMAYCEDMHYRTILINMRMYDK
                     WMDTWYDDEEKLQSVLKPAIRELISIKVDGIIYVAGHARLANCFPDDFEVPAIVVYAY
                     SKSPNFASVVIDDEKGGYDMTRYLISRGHRKIGVIAGTADNLHTQKRILGLQKALYEE
                     RIPFNPDWIKYGNWKRDSGYKEAEGLIKEGVTAIFCLNDDMAAGVYDYLYEHGLKAGE
                     DISIAGYDNMEISGYLKPALTTNEIPFAQIGIKSAELMINLLNEEYEIKPGSEIIKIP
                     CQIIERDSVKKIK"
     gene            1787..>3124
                     /locus_tag="PLG72_10635"
     CDS             1787..>3124
                     /locus_tag="PLG72_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOJ81292.1"
                     /translation="MIRMVLDAEVRKGKISKNIYGHFSEHLGRCIYGGIWVGKDSPIP
                     NTDGIRNDVIEALKAIKIPVLRWPGGCFADEYHWMDGIGPYESRPTMINTHWGGVTEN
                     NHFGTHEFLRLCELIGAEPYICGNVGSGTVREMQQWVEYITFDGVSPMADLRKANGRE
                     EPWKLTYFGVGNENWGCGGNMRPEYYADEYRRYATYIRNFGGNRIYRIACGANAGDYR
                     WTEVLMREAGNMMDGLSLHYYTVPGDWNAKGSATDFDESKWFDTLRKTLHMEELVTRH
                     SVIMDHYDPEKRVGLIVDEWGTWYDVEPGTNPGFLYQQNTLRDALVAGINLNIFNNHC
                     DRVKMANIAQMVNVLQSVILTEGDRMVLTPTYHVFGMYKVHQDAELLPMALENEEYRF
                     GGNTIPQVSASASIDKDNRIHISICNLSHDKEAEIDCLLRSGDISSVSGTILTA"
BASE COUNT          937 a          680 c          860 g          647 t
ORIGIN      
        1 ccatccccct gccggcggag gtgaaacctg aggaggccaa agcggaatac agggacggca
       61 tcctgacagt tactgtgccg aagtcggagc catctaagtc aaaaggaagg aaaatcgata
      121 tacaataaca tactataaga aagcaaagtt gacagtaata agcagaggta aaacgaagca
      181 aaaatgatca aaaccaggta aacgatataa agtaaaaaag aacgaatcac caggtgatga
      241 tcatctcggt cgggcggttc tgtatggcag aaaccgcctg atttcgtgca atatggcttt
      301 atgtttatga tggttaattt cagcaattcc gcaaaatgtt gcataacagg gcattatttt
      361 tatatattct gccagttgaa ttgaccagtt ttacttggtt attattatga caaggtcaaa
      421 tatttaacaa cttaagatag tatgaacaga aagaggaaga aagcttgatt acacagaaag
      481 aaatcgcacg gatgtgcgga gtttcaacat cgaccatatc gaatgtcctg aacggaaagc
      541 gcaaggtcag tgaggccacc aggcagaagg tcctggaaat cgtgaagaag acaggttacc
      601 agccgaacta tttcgctcag gggatgagaa agcagaaaac gaacataatc ggcataatcg
      661 cagaggatct gatccagttc agttcccctc ccattatcga aaggatcatg gcgtattgtg
      721 aggacatgca ctacaggacg atcctcatca acatgcgcat gtatgataaa tggatggata
      781 cgtggtatga cgacgaggaa aaactgcagt cggtactgaa gcctgcgatc cgcgagctga
      841 tttcgataaa ggtcgacggg atcatatatg ttgccggcca cgccaggttg gccaactgtt
      901 tcccggatga tttcgaagtc ccggcgatag tcgtatacgc atattcaaaa tcgcccaatt
      961 tcgcttccgt cgtcatcgac gatgaaaagg gcggatacga tatgaccagg tacctcatct
     1021 ccagggggca caggaagatc ggcgtgatag ccggtactgc cgacaacctg cacacccaga
     1081 agaggatact cgggctgcag aaggcattgt atgaagaacg gataccgttc aatcccgact
     1141 ggataaagta cgggaactgg aagcgggatt caggctataa ggaagctgaa gggctgatca
     1201 aggaaggcgt tacggcgatc ttctgcctga acgacgacat ggccgctggc gtctatgact
     1261 acctgtacga acacggtctg aaggcgggag aagacatatc catagccgga tatgacaaca
     1321 tggagatttc cgggtatctg aagcccgccc tcacgacgaa cgagataccg ttcgcacaga
     1381 taggcataaa atctgcggag cttatgataa acctcctcaa cgaagagtat gagatcaaac
     1441 ccggttcgga gatcataaaa ataccgtgcc agataatcga acgtgattct gttaagaaaa
     1501 taaaatgata tatttatatt aaagaaagca gttgataaac ggcgggtcat cgtgttttgc
     1561 ggtgatgccg ccttttttgc gggcaaagga gtgcctgcag catgatcgat agttgttgac
     1621 atgccacata aatgggaata taatttttca taggaagtaa aacgatttac taccaagtat
     1681 aaaaaatcac aaattggaaa tcaaatactt agagacttgg tgattcgaaa gccggcaagg
     1741 ctgtttgcga aacacaatac aaacaggaga gggatggata tacgacatga tacggatggt
     1801 attggacgct gaggtcagga aggggaaaat aagtaaaaac atctacggcc atttttctga
     1861 gcatcttggc agatgcatat acggagggat atgggtcggc aaggattccc ccatcccgaa
     1921 tacggatggc atccgcaacg atgtcattga agcactgaag gcgataaaga taccggtcct
     1981 gcgctggcca ggagggtgct ttgcggatga gtaccactgg atggacggta tcgggccata
     2041 tgagagccgg ccgacgatga tcaacacgca ttggggcggc gttactgaga acaaccattt
     2101 cggaacgcat gaattcctca ggctgtgtga acttatcggc gccgaaccgt acatctgcgg
     2161 gaatgttggc agcggcacag taagggaaat gcagcagtgg gtcgaatata taacgtttga
     2221 cggggtatcg ccgatggcgg acttgaggaa agccaacggg agagaagagc cgtggaagct
     2281 gacatacttc ggcgtaggca acgagaactg gggatgcggc ggcaacatgc ggcctgagta
     2341 ttatgccgat gaataccgca ggtatgcgac gtatataagg aacttcggcg ggaacaggat
     2401 atacaggatc gcctgcggag ccaatgccgg agactacagg tggaccgaag tcctgatgag
     2461 ggaggcaggc aatatgatgg acgggctcag cctgcactat tatacggttc ccggcgactg
     2521 gaacgcaaag ggctcggcta ctgattttga cgaatcaaaa tggttcgaca cgctcaggaa
     2581 gacgctgcat atggaagaac tggtgacgcg ccattccgtc atcatggacc attatgatcc
     2641 tgagaagagg gtcggcctga tcgtagacga atggggaaca tggtatgacg tggaacctgg
     2701 caccaacccg ggcttcctgt accagcagaa cacgctgagg gatgcgctgg tggccggcat
     2761 caacctcaac attttcaaca accactgcga cagggtgaag atggcgaata tcgcccagat
     2821 ggtcaacgta ctgcagtccg tcatactgac ggagggcgac aggatggtcc tgacgccgac
     2881 ataccacgta ttcggcatgt ataaggtgca ccaggatgca gaactgctgc cgatggcact
     2941 ggaaaacgaa gaataccgtt tcggcggcaa tacgatcccg caggtcagcg catcggcatc
     3001 gatcgataag gacaacagga tacacatctc catctgcaac ctgagccatg acaaagaagc
     3061 ggagatcgat tgcctgctgc gcagcgggga tatctccagc gtcagcggta ccatacttac
     3121 cgct
//
LOCUS       DAOSCC010000251         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20037_length_3120_cov_3.923980, whole genome shotgun sequence.
ACCESSION   DAOSCC010000251 DAOSCC010000000
VERSION     DAOSCC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_3120_cov_3.923980"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLG72_10640"
     CDS             complement(<1..1275)
                     /locus_tag="PLG72_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOJ81293.1"
                     /translation="MNRTSPDWNTPVYNALKSYSASGTLPFHMPGHKLGSGIPVHFLS
                     EIEKLDLTEMPGTDDLHRPEGVLKEAQELAAAAFGAAGSRFVVNGSTVGLHAAIAAVC
                     RPGQRLITGRDCHSSVVSGMLMSGVSPYYIMPGYSDEFCISTGITPDDVEKAMDDAPD
                     APAVLVTRPNYYGICCDIRRIADTVHARGKVLIVDEAHGPHLVFNKRLPVSALEAGAD
                     ICVQSAHKTLPAFTQGAYLHIGSDRADTERIDYFLDIYQTTSPSYIIMAFLDIAREIM
                     QKQGKELLDRLIDSIQANKTRLDGSTLHLLDKAAVPGFDHDPTRITVNVSRLGITGYE
                     AERLLRREYRIQAEMSDLSNLVFISTVADDPGSMERLFDALIGLQRLYTGAVTANGGT
                     GRSNAPDRACLGQRYNALLKRQIESCRRELYES"
     gene            complement(1277..2053)
                     /locus_tag="PLG72_10645"
     CDS             complement(1277..2053)
                     /locus_tag="PLG72_10645"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ81294.1"
                     /translation="MLLAINVGNTNIRFGVYEGKKLTAHWKLATNRGWTADEFGMYFI
                     NFFNYEKLKVSDVEAVIISSVVPPIMFSFEHAIEKYLKKKPIIIGPGIKTGINIKYEN
                     PRELGADRIANAVAAYEIYGGPAIVVDFGTATTFNAISSKGEFLGGAICPGILISAEA
                     LYQRTAKLPKIDLAKHGTAIGRNTAVGMQSGIFHGYVGQVNHLVNKIKQEMGEENIKV
                     IATGGLAKFIAPEAETIDEVNGRLVLEGLRIIYEKNRNEV"
     gene            complement(2151..2591)
                     /locus_tag="PLG72_10650"
     CDS             complement(2151..2591)
                     /locus_tag="PLG72_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81295.1"
                     /translation="MVRNRNEDSDGSIVKTEASQGNREQSRGRPNSRGKFRGRQQNMA
                     KNEEQGGKDMQGTSAVGQAHSQGAQKSPKSHDAYNRENSRQRGFYRDKGKGADRDHQK
                     YAGRHGGSYKNREEETIDDIRRDIERIEKEIELEIKEIRSMKFL"
     gene            complement(2801..>3120)
                     /locus_tag="PLG72_10655"
     CDS             complement(2801..>3120)
                     /locus_tag="PLG72_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81296.1"
                     /translation="IPDGQKNAHDEQAGRLALIRALLREIDSVYHHILSGNTDEILDE
                     WRKYSVTLGKEVRFSLKDTEYTGTAVDITESGSLLVDCSDGVRRELLSGEISIRGIFG
                     YSG"
BASE COUNT          625 a          871 c          753 g          871 t
ORIGIN      
        1 gctttcgtac agctcccgcc ggcaggattc gatctgcctc ttcagcaatg cgttatacct
       61 ttgccccagg caagcccggt ccggcgcatt gctccgccct gtgccgccgt ttgccgtgac
      121 cgcgccggta tacagccgct gcaggccgat cagcgcatcg aacagccttt ccattgatcc
      181 cgggtcgtct gccacggtac tgataaaaac caggttggac agatccgaca tttcggcctg
      241 tatgcggtat tcgcgcctaa gcagcctttc agcttcataa cctgttatgc caaggcgcga
      301 cacgttcact gttatccttg tgggatcatg gtcgaaaccg ggtaccgccg ctttgtcaag
      361 cagatgcagg gtactgccgt ccagcctggt cttattggcc tgtatcgagt cgataagcct
      421 gtccagcaat tccttgccct gtttttgcat tatttccctt gctatgtcaa gaaaagccat
      481 tattatgtac gatgggctgg tagtctgata tatgtcgagg aagtaatcga tcctttcagt
      541 gtctgccctg tcggaaccga tatgcagata cgccccctgc gtgaacgcag gcagcgtctt
      601 gtgcgcgctt tgtacgcaaa tgtccgcgcc ggcttcaaga gcggataccg gcagtctttt
      661 gttaaagaca agatgcggtc cgtgggcttc gtccacgatc agtaccttgc cgcgggcatg
      721 tacggtatcg gcgatcctcc tgatatcaca gcagataccg taatagtttg gtctcgtgac
      781 aagcaccgca ggcgcatcgg gcgcgtcgtc catcgctttt tccacatcat ccggggtgat
      841 gccggtgctg atgcagaatt catccgaata tccgggcatg atataatacg gcgaaacgcc
      901 ggacataagc ataccgctga ccacggaact gtggcagtcc cggccggtga tcaggcgctg
      961 gcccggcctg catactgctg cgattgccgc atgaaggcca acagtgctgc cattgacaac
     1021 gaaccggctt cctgctgcgc caaatgcagc cgcagccagt tcctgcgcct ccttaagcac
     1081 gccctcgggc ctgtggagat cgtcggtgcc gggcatttcg gtcagatcca gtttttctat
     1141 ttctgaaaga aagtgtacgg ggatgcctga tccgagctta tggccaggca tatggaacgg
     1201 aagcgtccct gaagccgaat atgacttcag ggcattgtaa acaggcgtgt tccagtcagg
     1261 cgacgttctg ttcatttcag acttcgttcc tgtttttttc atatatgatc cttaagcctt
     1321 ccaggacgag gcggccgtta acttcatcga tggtctcggc ttccggcgcg atgaatttgg
     1381 ccagtccgcc cgttgctatt acttttatgt tctcctcgcc catttcctgc ttgatcttgt
     1441 ttacaagatg gttcacctgt cccacatatc cgtggaatat tcccgactgc atacctactg
     1501 cggtgtttcg gccgatcgcc gtcccatgct ttgccaggtc tatcttgggc agcttggccg
     1561 tcctctggta cagcgcttcc gcagatatca ggataccggg acagatggcg ccgccaagga
     1621 attcgccttt cgacgatatg gcgttgaatg tagtggcagt gccgaaatcc actactatcg
     1681 cagggcctcc gtagatctcg taagcggcta cggcgtttgc gatcctgtcc gctccgagtt
     1741 cgcgcggatt ctcgtacttt atatttatac ctgtctttat gccggggcca ataattatcg
     1801 gtttcttttt tagatatttt tcaatggcat gctcgaaaga aaacattatc ggaggaacaa
     1861 cagacgatat gatgactgct tccacatctg aaactttgag tttttcgtaa ttaaaaaaat
     1921 tgatgaagta catgccgaac tcatcggctg tccatcccct gttggtagcc agtttccagt
     1981 gggctgtcag tttctttcct tcatatacgc caaaacgtat gttggtattt ccgacattta
     2041 tcgcaagcag caacttcaat tcgcctccag acaggcatca tgcaaaccag aggtgatatc
     2101 ccctgttgcc tcatctgcct gataatcttc ctgcccgcag ggcagaaaca ttacaggaac
     2161 ttcatgctcc tgatctcttt tatctcgagt tctatttctt tttctatcct ctctatatcg
     2221 cgcctgatat catcgattgt ctcttcttcc ctgtttttat acgaaccgcc gtgcctcccg
     2281 gcatatttct ggtgatccct gtccgcgccc tttcccttgt cgcggtaaaa ccctctttgc
     2341 ctggagtttt cacggttgta agcgtcatgg ctttttggac ttttctgcgc gccctgggaa
     2401 tgggcctggc cgactgcaga tgtaccctgc atatccttgc ctccctgttc ttcgtttttt
     2461 gccatgtttt gctgacgacc cctgaacttc cccctggaat tcgggcggcc gcgggactgt
     2521 tctctgttcc cctggcttgc ttcagtcttc acaatgctgc catcgctatc ttcgtttctg
     2581 tttctgacca ttcttaacct ccaaaatagt gaagcaatgt atttattgta ttttaaatgc
     2641 ttcatcaagt caaccccggt actttacggc gctgcaggct ggagtaactg tgcagtgaaa
     2701 gtaagtttca gcggaatgaa gcttaaaggt gtatagatgg tgcgaggata tataagacgg
     2761 ctgtctgagc aaaaacgccc tgccggaatt gtaaacgccc ttagcctgaa tagccgaaaa
     2821 tgccgcgaat ggatatctcc cctgacagca gttccctccg gacaccgtcg ctgcagtcta
     2881 ccaggaggct tccgctttcg gtgatgtcaa cggctgtgcc cgtatattcg gtatccttaa
     2941 gcgaaaacct cacttccttc ccgagggtga cggaatactt cctccattca tccagtattt
     3001 catctgtatt cccgctgagt atatgatggt aaacactatc gatctcccgg agcagcgccc
     3061 gtatcagggc cagccttccc gcctgttcgt catgtgcgtt tttctgcccg tccgggatgg
//
LOCUS       DAOSCC010000252         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20067_length_3117_cov_3.497387, whole genome shotgun sequence.
ACCESSION   DAOSCC010000252 DAOSCC010000000
VERSION     DAOSCC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20067_length_3117_cov_3.497387"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..362
                     /locus_tag="PLG72_10660"
     CDS             108..362
                     /locus_tag="PLG72_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81297.1"
                     /translation="MKIKIDAGGHRFNISLPIGIIMNRFTARIAGNCIKKYVSDPEVS
                     ITGEQLTILMRAMKQAKKNFPGLALVDVLTADRQRVLITF"
     gene            481..2340
                     /locus_tag="PLG72_10665"
     CDS             481..2340
                     /locus_tag="PLG72_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ81298.1"
                     /translation="MFLIKNKYGLPDMIILPFKVSPFYSAVFAVQRIVSGLMPTFTIF
                     VTAKFVNTAIAILNKTAELRDIYVPIALLAGIMLYGVVIGVLMEFVNCRANIVFRKKL
                     RPEMAEKRARLEYRHIEDQNTADLINRVCPVIDTTVKDMYMRVLDIAENLIYAAGILV
                     ALFTQVWWVALFLAVSGVPILTIATKAGERSYEADREMSKIDRRTWYLSDVLKSREAV
                     EERSVYGYSGKLNEQFAERYEFARKFRMKVSIKNFIKQKMGGIITSILSTAVMLAMLP
                     PVVQGKIDIGMFIGMMGGVFSLSGKLSWGVNWIIEDLARKKEYLKDLTEFMKLEEHDD
                     ANALPDRTVNFRTIEFRNVSFRYPGTEKLILDNISFVIESGKHYAFVGVNGAGKTTVT
                     KLITGLYSNYEGEILVDGRPLRSMSQAEIKGLSSVVYQDFAKYYISMYDNIALGCLNQ
                     EHDRADIENAAELAGLSDTIERLPDGMDTPLGKIQEKGVDISGGEWQRVAIARGIASS
                     APLRILDEPTASLDPVSESMVYKKFEQISRGMTTIFISHRLGSTKLADIIYVIADGRI
                     AESGSHEELMEKNGIYAEMFRAQAEWYKTGESDGPLHVTGRPQMNETGVAANA"
     assembly_gap    2387..2396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2397..>3117
                     /locus_tag="PLG72_10670"
     CDS             <2397..>3117
                     /locus_tag="PLG72_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81299.1"
                     /translation="PKKAYKCTKKENERPKVSLLKVILFGLKVNMSALPGLFICINIV
                     SIFHGISHGFATFMTQNFYDSVENVLTGKGSIRQACMMIAALGLTCIVRELLNGLHNF
                     MHDVVLTKLNGEMAKIIHGKMARIDPVCFEDTRLHDDINKAQEGAWTVAFILNIGVTI
                     FTFYIPYFAFMGVYLYYLKPQFILAIVMVFIPVLAGQLMRTGIISKFEDKAAPVRREY
                     EYYEKAVADREYFKETRILGAF"
BASE COUNT          857 a          679 c          849 g          722 t
ORIGIN      
        1 gcaatattgc tggcagcgga gagcggagtc atcggcaaac tggtggaagt caaaagcgct
       61 gatggggatg tcgtcgaaat cgtgatcgag tgatgcggag gtttcttatg aaaatcaaga
      121 ttgatgcagg agggcatcgc tttaacatat cgctgccaat cggcatcata atgaacaggt
      181 ttacggccag gatagcaggg aactgcataa aaaaatacgt ttccgacccc gaagtttcta
      241 tcacgggcga gcagcttacc attctgatgc gggcaatgaa gcaggccaaa aagaattttc
      301 ccggccttgc tcttgttgat gttttgactg ccgacaggca gagagtattg attaccttct
      361 gacaaatgaa ttttacataa gcctggttat ttgaaatact gactgaacaa catcagaccg
      421 gattgagctt tcttcgtatg attcatcaga agtggtttac ataattgcag gaggtacgca
      481 atgtttctga tcaagaacaa atatggcttg cccgatatga tcatccttcc ttttaaagta
      541 tcgccctttt attcagcggt atttgccgta cagcggatcg tgagcggcct gatgcctaca
      601 tttacgatat ttgttacggc aaaattcgtc aatactgcaa tagcaatatt aaataaaacg
      661 gcggaactgc gtgatatata tgtcccgata gctttgcttg cgggcataat gctctacgga
      721 gtcgttatcg gcgtcctgat ggagtttgtg aactgcaggg caaatatagt tttcaggaag
      781 aaactgcgtc ctgaaatggc cgaaaagcgt gcgagacttg aatacaggca tatagaagat
      841 cagaatacgg ccgacctgat aaaccgggtc tgccccgtga tcgacactac tgtaaaagat
      901 atgtacatga gggtcctgga tatagctgaa aatttgattt acgctgcagg catcctcgtt
      961 gctttgttca cacaggtctg gtgggtcgca ctgtttttgg ctgtatcggg cgttcccatc
     1021 ctgaccattg ccacgaaagc cggtgagagg agctatgaag ctgacaggga gatgtcaaag
     1081 atcgaccgca ggacatggta cctgtcagat gtgctcaaaa gcagggaagc ggtcgaagaa
     1141 cgcagtgttt atgggtacag cggcaagctg aacgaacagt tcgctgaacg gtatgaattt
     1201 gccaggaaat tcaggatgaa ggtttcgatc aaaaatttca taaagcagaa aatgggcgga
     1261 atcatcacat cgatactgtc gacggccgtc atgcttgcga tgctgccgcc tgtcgtccag
     1321 ggaaagatcg acattggtat gtttatcgga atgatgggcg gcgtcttcag cctttcaggc
     1381 aagctgtcgt ggggcgtgaa ctggatcatc gaagaccttg cgcggaagaa ggaatacctc
     1441 aaagatctta ccgagtttat gaaacttgag gaacacgatg acgccaatgc cctgcccgac
     1501 aggaccgtga atttcaggac tatcgagttc aggaacgtat cgttcaggta tccgggaacg
     1561 gagaagctta tcctcgataa catatctttc gtgatagaaa gcggcaaaca ttacgccttt
     1621 gtcggggtga acggagccgg aaaaacgacg gtcacaaaac tgatcacggg actgtattcc
     1681 aattacgaag gagagatcct ggtggacggc cggcctctcc ggagcatgtc tcaggcagag
     1741 ataaaaggtc tgtcatcggt ggtataccag gattttgcga agtattacat atccatgtac
     1801 gacaacatag cattaggctg cctgaaccag gagcatgacc gtgctgatat cgaaaacgca
     1861 gcggaactgg cagggctgtc cgacacgata gaaaggctcc ctgacggcat ggatacgcca
     1921 ttgggcaaga tacaggaaaa aggcgtcgac atttccggcg gcgaatggca gcgtgtggct
     1981 atagccaggg gcatagcaag ttccgctccg ctcaggatac tggatgagcc tacggcttcg
     2041 ctcgaccccg taagcgagag catggtatat aagaaatttg aacagatctc aagaggcatg
     2101 actacgatat tcatcagcca taggctgggc tcaaccaagc ttgcggatat catctatgtc
     2161 attgccgacg gcaggatagc tgaaagcggg tcccacgaag agcttatgga gaaaaacggg
     2221 atatatgcgg aaatgttcag ggcgcaggct gaatggtaca agaccggcga gtccgacgga
     2281 ccgctgcatg taacgggacg gccgcagatg aatgagacgg gggtggctgc aaatgcgtaa
     2341 ggagaagaaa aaggatgaac gtccgaaggt ctcgctcatg aaggttnnnn nnnnnnccga
     2401 agaaagctta taagtgcacg aagaaggaaa atgagcgccc gaaggtctcg ctcctcaagg
     2461 tcatcctgtt cgggctgaaa gtgaatatgt ccgccctgcc cggcctgttt atctgcataa
     2521 acatcgtaag cattttccat gggatctccc atggcttcgc aacattcatg acccagaatt
     2581 tttatgattc ggtggaaaac gtactgacag gcaaaggctc gataaggcag gcctgcatga
     2641 tgatcgcagc cctcggactg acatgtattg tcagggagct gctgaatggc ctgcataatt
     2701 tcatgcatga tgttgtttta acaaaactga acggggagat ggcgaaaatc atacacggca
     2761 aaatggcgcg tatcgacccg gtatgctttg aagacaccag gctccacgac gatataaaca
     2821 aggcccagga gggagcctgg acggtagcct ttatcctgaa tatcggcgta acgattttta
     2881 ccttctatat cccttacttc gcttttatgg gagtgtacct gtactacctg aaaccgcagt
     2941 tcatcctggc catagtgatg gttttcatac ccgtgcttgc aggacagctc atgcggacgg
     3001 gcataatatc gaagtttgag gacaaggccg ctccggtcag gcgggaatat gaatattacg
     3061 aaaaggcggt cgccgacagg gaatatttca aggaaacgcg gatcctgggg gcattcc
//
LOCUS       DAOSCC010000253         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20275_length_3093_cov_5.266952, whole genome shotgun sequence.
ACCESSION   DAOSCC010000253 DAOSCC010000000
VERSION     DAOSCC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20275_length_3093_cov_5.266952"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1159)
                     /gene="lgt"
                     /locus_tag="PLG72_10675"
     CDS             complement(89..1159)
                     /gene="lgt"
                     /locus_tag="PLG72_10675"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ81300.1"
                     /translation="MDLHNISFPGLGLEFELRRYAFIVFGIKIYWYGIIITLAFLAAV
                     VMALRSCKKYDLTADNILDLVLFAAPVAIIFSRLYYVVFNWGQFRNDLIAIFKIRDGG
                     LAIYGAVIGALLVAWLYTRKKKISFLNLVDFGIPYVAFGQGIGRWGNFINQEAFGEIT
                     DLPWRMNGDEINRFILTEGADPSVFGVHPTFLYESVLDIAVFLILLYWRKKKKVEGEV
                     LFLYFILYGTGRALIEGLRTDSLMLGSLRVSQVLSVILVLAGLFLFLYRRAQKRKSEE
                     ETVVIGQSRYGALLMQMKAEEEAEKSAAGLSEATSDGIGTAAGDTTNTDLSNETGEET
                     AGTVSGDGLSEKIEETEPAEDK"
     gene            complement(1225..2508)
                     /locus_tag="PLG72_10680"
     CDS             complement(1225..2508)
                     /locus_tag="PLG72_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ81301.1"
                     /translation="MDFEKIEYKKAGEVLYSYEHDSGLKAFVIPRRGYLKKYAAFATN
                     YGSIDSEFIIPGQEDVTRVPDGIAHFLEHKLFEQKDGNVMEKFSRLGSSPNAYTSFNK
                     TVYLFSCTDRFDENFSLLLDYVQNPYITQESVENEKGIIGQEIMMYQDNPDWKVHFNL
                     LKALYEKHPVRIDIAGTIDSISRIDRDTLYKCYNTFYHPSNMIILAVGDVDPESVFEM
                     VEGSIPHSKPQPPVSRIYPEETGAVHTDYIEERLAVSIPMFQMGYKGGFYGEKGIELL
                     MYETAVKLVLELLIGRSSDLYEQLYSEGLINSSFGADASVEKQYAFSVLGGESPDPVQ
                     VRERFCHVLETAKKNGLDRAACERLIRSFRGRFMRQFNSVERISHSFISVYFKGVSMF
                     DYLDVYDKITFEYVNEVLHEHFKPESLAMSVIRPV"
     gene            complement(2542..>3093)
                     /locus_tag="PLG72_10685"
     CDS             complement(2542..>3093)
                     /locus_tag="PLG72_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOJ81302.1"
                     /translation="RHVTEEMNVTQGKLCLGCRTNTAPDSDDYPALMVYNGILGGGIH
                     SKLFRNVREKASLAYYCYSMLEKFKGLMVISSGIDISNKQKALDIMEEQLDDMAKGNI
                     SDYEMEATVKSMETGIKSLTDSQMNIVDFYLSQTIAGTRDDFSTIMEKIRKVTKDDVK
                     AAAQRIVRDTVYFLTAADSAGNQ"
BASE COUNT          751 a          828 c          654 g          860 t
ORIGIN      
        1 ccctgtcgat ccatcaggct gtgcaatgag cacatacgag atcagtaatc atttgcctta
       61 accgcaggca gcagcaacgc cgttcggact acttatcttc tgcgggttcc gtttcctcga
      121 ttttttcaga caagccgtcc ccggaaacgg ttccggccgt ttcttccccg gtttcattac
      181 ttaagtcagt atttgtcgtg tctccggcag cagtgccgat accatcggat gttgcttcac
      241 tgaggccggc agcagacttt tctgcttctt cctccgcttt catctgcata agcaacgcgc
      301 cgtaccggct ttggccaata acgacagttt cttcttccga ttttctcttt tgggcacgcc
      361 tgtacaggaa caggaataga ccggctaaga caaggatcac ggaaaggacc tgcgaaaccc
      421 tcaaactgcc cagcataagg ctgtctgtcc ttaagccttc tatcagcgcc ctgcccgtac
      481 cgtaaagtat gaaatacagg aagaggactt ctccctccac ctttttcttt tttctccagt
      541 acagcaggat caaaaataca gcaatatcca ataccgattc atacagaaaa gtcgggtgca
      601 ctccgaatac cgaagggtct gcgccctcag tcagtataaa tctgttgatt tcgtccccgt
      661 tcattctcca ggggagatca gttatttcac caaaagcttc ctggtttata aagttgcccc
      721 agcgtcctat cccctgtccg aatgcaacgt aaggaatgcc gaaatcaaca aggttcagga
      781 acgatatctt cttcttcctg gtatacagcc acgccaccag aagtgcgccg ataactgccc
      841 cgtaaatcgc gaggcctccg tctctgattt taaagatagc tatcagatcg tttctgaact
      901 gaccccagtt gaaaactaca taatagagcc ttgaaaatat gatggcaaca ggcgctgcaa
      961 agagaaccag gtccaggatg ttgtctgccg taaggtcata ctttttgcag cttctcaaag
     1021 ccatcacgac tgccgcaagg aatgccagtg tgattattat accgtaccaa tatatcttga
     1081 tgccaaaaac aatgaatgca tatcttctca gttcaaactc gagtcctaac cctggaaagc
     1141 ttatgttatg caaatccaca gctaatcccc ctcatactcg caaataaaaa catcatttaa
     1201 gtataccgac tgcaggagcg gagtttatac aggcctgatc actgacatag cgaggctctc
     1261 aggcttgaaa tgctcatgga ggacctcatt cacatattca aatgttattt tatcataaac
     1321 gtccaaatag tcaaacatgc tgacaccctt aaaatacact gatatgaacg aatgcgagat
     1381 cctttccacg gaattgaact gcctcatgaa ccggccgcgg aaagatctga tcagcctttc
     1441 acaagccgct ctgtcgagcc cgtttttctt cgctgtctcc aacacatggc agaacctctc
     1501 cctcacctgt accgggtcag gcgattcgcc tccaagtact gagaatgcat attgcttttc
     1561 aacactggca tcggcgccaa aggatgagtt gatcagacct tcgctgtata actgctcata
     1621 aaggtcggaa cttctgccga tcagcaattc caggacaagt ttcacagcag tctcatacat
     1681 cagcagttcg atgccttttt cgccgtagaa accgccttta tagcccatct ggaacatggg
     1741 gatcgacacg gcaaggcgct cttcgatata gtcggtgtgg actgccccag tctcctcagg
     1801 atagatcctg ctgacaggag gctgcggttt gctgtgcggg atcgagcctt ccaccatttc
     1861 gaatacgctt tcgggatcaa catcgccgac tgcaagtatg atcatattcg aaggatggta
     1921 aaacgtgttg tagcatttgt acagtgtatc cctgtcgatc ctgctgatgc tgtcaatggt
     1981 tccggcaatg tcgatcctga cagggtgctt ctcatacagt gctttcaaca ggttgaaatg
     2041 caccttccag tcagggttat cctgatacat cattatctcc tgtccgatta tgcccttttc
     2101 gttttcgacg ctctcctgcg ttatatacgg gttctggaca tagtccagca gcaggctgaa
     2161 attctcatcg aacctgtcgg tacatgaaaa gaggtacacc gttttgttga aactggtgta
     2221 agcgtttggg cttgaaccca gtcttgagaa tttctccatt acattcccgt ctttttgctc
     2281 aaagagctta tgttcaagga aatgtgctat accgtccgga acacgggtca catcctcctg
     2341 tccggggatg atgaactcac tgtcgatgga accgtaatta gttgcgaagg cagcgtattt
     2401 tttcagatat ccccgccttg gtatcacaaa ggccttcagt ccgctgtcat gttcataact
     2461 gtaaaggact tcgccagctt ttttgtattc gatcttctca aaatccattt aaacaactcc
     2521 tcattacata ctttcatatg gttattggtt tcccgcgctg tcggcagcag taaggaaata
     2581 gaccgtgtcc cggacgatgc gctgcgctgc cgctttgaca tcatcctttg ttacctttct
     2641 gatcttttcc attattgtgc tgaaatcgtc ccgggtgcct gcgatcgtct ggctgaggta
     2701 gaaatcgacg atattcatct ggctgtccgt caatgatttg atccccgttt ccatgctctt
     2761 tacagtcgct tccatttcat aatccgatat attgcccttc gccatatcgt caagctgctc
     2821 ttccattata tccagcgcct tttgcttatt gcttatatcg ataccgctgc tgatcaccat
     2881 aagccctttg aatttttcaa gcattgagta gcagtagtaa gccaggctcg ccttttcccg
     2941 gacgttccgg aatagcttcg agtgtatgcc gccgcccagt atcccgttat agaccatgag
     3001 cgcagggtaa tcatcactgt caggagcggt gttcgtccga cagccgaggc aaagcttccc
     3061 ctgcgtcaca ttcatctctt cagtcacatg cct
//
LOCUS       DAOSCC010000254         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20487_length_3070_cov_4.200663, whole genome shotgun sequence.
ACCESSION   DAOSCC010000254 DAOSCC010000000
VERSION     DAOSCC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20487_length_3070_cov_4.200663"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..360
                     /locus_tag="PLG72_10690"
     CDS             109..360
                     /locus_tag="PLG72_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81303.1"
                     /translation="MDLFMINALEAIGITLIIVCPFMDNEIQDRDREDQAGRGSAQGR
                     DQVSIRLLQMIKVMGACTGQGSENKLRAYKNGWNHALIL"
     gene            1079..2098
                     /gene="pheS"
                     /locus_tag="PLG72_10695"
     CDS             1079..2098
                     /gene="pheS"
                     /locus_tag="PLG72_10695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ81304.1"
                     /translation="MREQLNNIKLEAEKKLNGAGSLAELESIRLQYLGKKGELTAVLR
                     GMGQLTPEERPQIGQLANEVRSFIEEMLEEKTKSLQEKERQAKLEKEAIDVTVPGRRR
                     FIGKKHPLSIVLDEIKDVFIGLGYEIAEGPEVELDYYNFEALNIPAGHPARDTQDTFF
                     INGDILLRSQTSPVQIRVMEKKKPPIKIICPGRVYRSDAVDATHSPIFHQVEGLVVDR
                     GVTMGDLTGTLQYFARRLFGEDTKIRLRPHHFQFTEPSAEVDVSCWACGGKGCRICKD
                     EGWIEILGAGMVHPNVLKYGGIDPKEYSGFAFGLGVERVAMCKFNIDDMRLLYENDMR
                     FLKQF"
     gene            2248..>3070
                     /locus_tag="PLG72_10700"
     CDS             2248..>3070
                     /locus_tag="PLG72_10700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ81305.1"
                     /translation="MKLPLKWLKDYTDIDASPREYAHALTMSGSKVEGLEVLGSGIDR
                     VVVGKILTVEKHPDADRLQVCQVDVGSGTIQIVTGATNIKVNDLVPVALDGSSLPGGI
                     RIKKGKLRGVESAGMMCSIAELGLTKDDYPNAAEDGIFILEGDPDPGTDIKEYLGMND
                     TVVEFEITSNRPDCFSIIGLARESAATFGTSFRIPEVAVKELGEPIDGKVSVEIKAPD
                     LCARYAARIVENVKIGPSPRWMQQRLRASGIRPINNIVDITNYVMLEYGQPMHAFD"
BASE COUNT          837 a          691 c          925 g          617 t
ORIGIN      
        1 tgtatttacc tttctgcgat ttgaatatat aataattaac aaatcggcct gacgatggaa
       61 aatggaaatg atatatacaa gtattacggg gattttggag gtacagacat ggacttgttc
      121 atgataaatg cactggaggc catcgggata acgcttatta tcgtgtgtcc cttcatggat
      181 aacgaaatac aagatcgaga tcgagaagat caggcaggac gcggaagtgc gcaaggaaga
      241 gatcaggtgt cgatacggct gctgcagatg ataaaagtaa tgggcgcgtg tacaggacag
      301 ggcagtgaga ataaattgcg ggcatacaaa aacggatgga atcacgcctt gattttatga
      361 aacatattgt atataatacc gtaaatgatg tataatctat agaacattat tgactggtaa
      421 aagcaatgaa agaggaaagt aaccggaacg tactttacag agaggagctg cccccggctg
      481 aaagcagcgc caatgtgcac accggcgaag ttccctccgg agctgcatcc tgaaaaccca
      541 gtgctatgcg gcgtcggtat acgggtcggg accccgaccg atgtatatcg gtctttaaca
      601 ggtgccgggc cgcttcagac ggctgtacag cgggataagt aggagatgcc ggcgcctggc
      661 cgttaacata caggaagcga tttgcctgat ctgcctgcgc agtgatgaag tcgcgcaaga
      721 gtgtccgttt ttgcggaaat ttgggtggta ccgcggagtt gcagccttcg tccctttcat
      781 ggggacggag gcttttatta tatataaatg atgcggtttt gtataacgca tcaggttggt
      841 acggccctgg taccataggt tatggctttg gtacgcagtg ttatgcatca tcgtgttaca
      901 gatttttgtg caacgtatca acgtgatgca atttttcacc gccggtcggc cgggtgtgtt
      961 attgcagtgc aggacccctg cgcggcaggg ttgacgccag gagcccgggg aaccggatcg
     1021 aaagcaagtt atggcaaatg actggaattg cagcgaataa caagaaggag gaagcgcaat
     1081 gcgggaacag ttgaacaaca taaagctgga agcggagaag aagctgaatg gagccggttc
     1141 gctggccgaa ctggaaagca tccggctgca gtatctcggg aagaaaggcg aactgactgc
     1201 agtcctcagg ggaatggggc agctcacgcc cgaggaaagg cctcaaatag gccagcttgc
     1261 gaatgaagtg cggagtttca tcgaggagat gctcgaggag aagacaaaga gcctccagga
     1321 aaaggaacgc caggcaaagc tggagaagga agccatcgat gtgacggtac ccggcaggag
     1381 gcgtttcata ggcaaaaaac atccgctgag catagtgctt gatgagataa aggatgtgtt
     1441 catcgggctt gggtacgaaa tagccgaagg ccctgaggtg gaactcgatt attacaattt
     1501 cgaagcgctg aatatccctg caggccatcc ggcaagagat acgcaggaca ccttcttcat
     1561 aaacggcgat atcctgctca ggagccagac gtcgccggta cagataaggg tcatggagaa
     1621 gaagaaacct ccgatcaaga tcatctgccc gggaagggtc tacaggtcag acgcggtgga
     1681 tgcgacacac tcgcccatat tccaccaggt ggaagggctg gtcgtggacc gcggcgttac
     1741 aatgggcgac ctgacgggga cgctccagta ctttgccagg agactgttcg gagaggatac
     1801 aaagataagg ctgaggcccc atcatttcca gttcaccgag ccgagcgcag aggtcgacgt
     1861 ttcatgctgg gcctgcggcg gcaaaggttg caggatatgc aaggacgaag gctggatcga
     1921 gatactcggc gcaggtatgg tccatccgaa cgtattgaag tatggcggga tagacccgaa
     1981 agaatacagc ggttttgcat tcgggctcgg agtcgaaagg gtagccatgt gcaagttcaa
     2041 tatcgatgac atgaggctgc tgtatgaaaa cgacatgcgt ttcctcaaac agttctgaga
     2101 tgcagccgga cagatcccgg gttgacagcg gcggcagccg gcatgaccgg gctgcacaaa
     2161 gcgtatgcag gaatgagctg cacgatacgg ctgcccggat gagccgcgca ataaggatat
     2221 aatacgcaag gaagggagat agttgagatg aaattaccgt taaagtggtt gaaagattat
     2281 acggacatag atgcaagccc aagggaatac gcccatgcat tgaccatgtc gggttccaag
     2341 gtggaaggcc tggaagtgct tggctccggg atagacaggg tggtcgtggg gaagatcctt
     2401 actgttgaga agcatcctga tgcggaccgc ctccaggtgt gccaggtaga cgtcggcagc
     2461 gggaccatcc agatcgtgac cggcgccacc aatattaaag tgaacgacct tgtcccggtg
     2521 gcgctggacg gctcgagcct gccgggaggg atcaggataa agaaaggcaa gctccgcggc
     2581 gtcgaatctg ccggaatgat gtgctccata gcggaacttg ggctgacgaa ggacgattac
     2641 cccaacgcag cggaggacgg cattttcatc ctggaaggcg acccggatcc gggtaccgat
     2701 ataaaagaat acctcggaat gaacgacacc gtggtggaat tcgagatcac atccaacagg
     2761 cccgactgct tcagtatcat cggcctcgca cgagaatcgg ccgccacatt cgggacatcc
     2821 ttcaggatac ctgaagtcgc tgtgaaggaa ctcggcgaac cgatagacgg taaggtctca
     2881 gtcgagataa aggcccccga cctgtgcgca cgttatgccg caaggatagt ggaaaatgtg
     2941 aagataggcc cttcgccccg ctggatgcag caaaggctca gggcgtcagg tatcaggcct
     3001 atcaataata tcgtagacat aacgaactat gtcatgctcg aatacggaca gcccatgcac
     3061 gccttcgatc
//
LOCUS       DAOSCC010000255         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_20519_length_3067_cov_4.184927, whole genome shotgun sequence.
ACCESSION   DAOSCC010000255 DAOSCC010000000
VERSION     DAOSCC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20519_length_3067_cov_4.184927"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="gnd"
                     /locus_tag="PLG72_10705"
     CDS             <1..1256
                     /gene="gnd"
                     /locus_tag="PLG72_10705"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOJ81306.1"
                     /translation="PRKIIIMVKAGQPVDDMIRQLKQYISKGDIIIDAGNSFFGDTIR
                     RSRELEAEGYRFVGTGVSGGEEGALRGPAIMPGCEKETYDMIAPILTAIAAKVNGDPC
                     CTYIGTDGAGHYVKMVHNGIEYGDMQLICEAYSLLKNILGMTAQELHEVFAEWNRGEL
                     DSYLIEITSDIFTKTDPETGKPMVEVILDKAGQKGTGKWTSKSALDLGISTPVITEAV
                     FARCISAIKDERAAASKVFKEPSVGAFSGDRKSFVESVRRALYASKICSYAQGFALMK
                     AAAQEYGWDLDFGRIAMIFRGGCIIRAQFLHRIKEAFDKDPDLANLMLAPYFKEILEK
                     YQEDWRKAVAAAVMHGIPAPAMSSALAYFDSYRSERLPANLLQAQRDYFGAHMFERVD
                     KPRGEFFHFNWTEHGGKTSASAYNA"
     gene            1413..2771
                     /locus_tag="PLG72_10710"
     CDS             1413..2771
                     /locus_tag="PLG72_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ81307.1"
                     /translation="MESITGKTIIMDDIIVIDDVCNGTKNDGTVADKGINDAELRKAL
                     ELSMERCRKSIGHPKKVLLIPPDFTRMHSGAGRITAIYYEMLKGSCHVDIMPALGTHE
                     PMTPAEIDEFFGNGVPHEAFIVHNWRKDVVKIGQVPADYVKQVSEGLVSDPVDVEVNR
                     RIVDGTYDLIISIGQVVPHEVVGMANYSKNIFVGCGGSGMISQSHMLGAFYGLERIMG
                     RDHSPVRKVFDYAQEHFLNSVLLMYVLTVTTQNEGKVAIHGLFAGSSRRIFEEAVKLS
                     QQKNIIFVDKPLKKAVVWLDEKEFKSTWLGNKAVYRTRMAIDDGGELVVIAPGVRKFG
                     EDDENDRLIRKYGYVGREKVLQLVREHDDLRNNLSVAAHLIHGSSDGRFRITYAPGRL
                     TKQETESVGFEYADIREAIGRYDPVKLKDGFNTMPDGEVIYYISNPALGLWVCRDRFA
                     HE"
BASE COUNT          821 a          707 c          902 g          637 t
ORIGIN      
        1 ctccgaggaa gatcatcata atggtcaagg ccggacagcc tgtggacgat atgatccggc
       61 aactgaagca gtatatttcg aagggcgaca tcataatcga tgccggcaat tccttcttcg
      121 gcgacacgat aaggaggagc agggaactgg aagccgaggg gtacaggttc gtcggtaccg
      181 gtgtttcagg cggcgaggag ggagcgctga gaggtcccgc catcatgccg ggatgtgaaa
      241 aggaaaccta tgatatgatc gctccaattc tgacggccat agccgccaag gtgaacggcg
      301 acccgtgctg cacatacata gggacagacg gcgccggaca ttatgtcaag atggtccaca
      361 acgggatcga atacggtgat atgcagctta tttgcgaggc atattcgctc ctgaagaata
      421 tccttgggat gacggctcag gagcttcatg aagtattcgc ggaatggaac aggggcgagc
      481 ttgacagcta ccttatcgaa ataaccagcg atattttcac gaagaccgat ccggaaacgg
      541 gcaaaccgat ggtggaagtc atactggaca aggcagggca gaaaggtacc gggaaatgga
      601 caagcaagag cgccctggac cttggtatct ccaccccggt cataacggaa gcggtctttg
      661 cccgttgtat ttcagccatc aaggatgaaa gggctgcagc aagcaaagta ttcaaagagc
      721 cgtccgtcgg tgcgttcagc ggcgacagga agagctttgt cgaatctgtg aggcgcgcat
      781 tgtatgccag caagatatgc tcatatgcgc agggattcgc ccttatgaaa gctgcggcac
      841 aggaatatgg ctgggatctt gatttcggca ggatagcgat gatattcagg ggaggctgca
      901 taatcagggc ccaattcctg cacaggatca aggaagcgtt cgataaggac cctgatctgg
      961 caaacctgat gcttgcaccg tatttcaaag aaatactcga aaaataccag gaagactggc
     1021 gcaaggcagt ggcggccgcc gtgatgcatg gcattccggc accggcgatg tccagcgcgc
     1081 ttgcatattt cgacagctac aggagcgaaa ggctgcccgc caatctgctc caggcacaaa
     1141 gggactattt cggagcccat atgtttgagc gtgtagacaa gccgcgcgga gaattcttcc
     1201 acttcaactg gactgagcat gggggcaaaa cttccgcttc cgcttataac gcctgaccgt
     1261 gcttatgcca atggatcatg gcaggggcaa ttttgaaatt cgggtacaac ggtttgatac
     1321 agcaagaagc gtgaccggaa aggtatggta cagtccgata tttcaggctg ccgtatgcct
     1381 tagccgcata tttatatgaa agggtgcttg ccgtggaaag catcactggg aaaacgatca
     1441 ttatggatga tatcatcgtc atcgatgatg tctgcaatgg cacgaaaaat gatggcactg
     1501 tcgccgataa aggtataaac gacgcggaac tcaggaaggc cctggaactg agcatggaac
     1561 ggtgcaggaa aagtatcggg catccgaaaa aggtgctgct gatcccgcct gatttcacaa
     1621 ggatgcattc aggtgcgggg cgaatcacgg ccatctatta tgaaatgctc aaaggcagct
     1681 gccatgtaga tattatgcct gccctcggga cccatgagcc gatgacgccg gcggaaatcg
     1741 acgagttttt cggcaatggc gttccccatg aggcattcat cgtccacaac tggaggaaag
     1801 atgtggtgaa gatcggccag gttcctgcgg attatgtgaa acaggtctcc gaagggcttg
     1861 tatcggaccc ggtggatgtg gaagtgaaca ggcgcatcgt ggacggaaca tacgacctga
     1921 tcatttccat cggccaggtc gtgccgcacg aagtggtggg aatggccaac tacagcaaaa
     1981 acatattcgt cgggtgcggc ggcagcggca tgataagcca gtcccatatg ctgggtgctt
     2041 tttacgggct cgagaggatc atgggacgcg accattcccc tgtacgtaag gtatttgact
     2101 atgcccagga acatttcctc aatagtgttc tcctgatgta tgtgctgacc gtgacgactc
     2161 agaacgaagg caaagtggca atccatggcc tgtttgcggg cagcagccgg aggatctttg
     2221 aagaagcggt gaaactcagc cagcagaaaa acattatttt cgtagacaag ccgctgaaaa
     2281 aggcagtcgt ctggctcgat gaaaaggagt tcaagagtac gtggctgggc aacaaggctg
     2341 tctacaggac caggatggct atagacgacg gaggcgagtt ggtcgtaatt gcgcctggcg
     2401 tcagaaagtt cggcgaagat gatgaaaacg accggctgat caggaaatac ggatatgtgg
     2461 gaagggagaa ggtactgcag ctggtcaggg aacatgatga cctcaggaac aacctctccg
     2521 ttgcggcgca cctgatacat ggttcatctg acggccgctt caggatcaca tatgctcccg
     2581 gcaggctgac gaagcaggag acagaatccg tcggctttga gtatgcggat atacgtgagg
     2641 ccatcgggcg ttatgatccc gttaaactaa aagatggctt caataccatg cccgacggcg
     2701 aggtaatata ttatatcagc aatccggcgc tcggtctctg ggtctgccgg gacaggttcg
     2761 cgcatgagtg aaacatgtca ggcacaagga aggaaactgt gccgccgagg gaaccgcgcg
     2821 gctttgaaat ttgactgcaa ggtgcaaacg gcatgcagca accaactgag tacctgaatt
     2881 ttggggtgcc aggcgcagat ggcgtgcagc agcccgcata aagacggctg tttacggata
     2941 cacgcgcaaa atgccagaca ttctgattta aaggagaatg tgttatgaag aaattcatgg
     3001 acgagaattt cctgctgcat aatgaaacag cagccatcct ttatcatgaa tatgctgctg
     3061 aaatgcc
//
LOCUS       DAOSCC010000256         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_21106_length_3003_cov_3.515604, whole genome shotgun sequence.
ACCESSION   DAOSCC010000256 DAOSCC010000000
VERSION     DAOSCC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21106_length_3003_cov_3.515604"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..529
                     /locus_tag="PLG72_10715"
     CDS             209..529
                     /locus_tag="PLG72_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOJ81308.1"
                     /translation="MKSHMHKFKFDCRLTNGHNHRLLGYASGMVGIGSFHFHFYYGVS
                     SYRDHTHYFCGVTGMPHKTENGHIHKMEGILEYNDSHEHVYKGYTFEEISYIPSASVI
                     GFVR"
     gene            complement(682..1215)
                     /locus_tag="PLG72_10720"
     CDS             complement(682..1215)
                     /locus_tag="PLG72_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ81309.1"
                     /translation="MAQTDMIFAGFGGQGIQAAGKLMAYAGIMEDKFVSFLPSYGPEM
                     RGGTSNCHVIVSDEPIGSPILNSATVVMAMNRPSMDKFENYIEPDGILLYDSSLVNRE
                     PARKDIRAFAIPATQIASDMGNLAFANIIMLGKLIKETGVVSPENFERGLKKVLPEKK
                     HYLIPDEMKALKIGMEY"
     gene            complement(1219..1965)
                     /locus_tag="PLG72_10725"
     CDS             complement(1219..1965)
                     /locus_tag="PLG72_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ81310.1"
                     /translation="MAIVFQRPHALRETQLHYCPGCTHGIVHRLIAEVIDELGIEGKT
                     IGVSPVGCAYNNYLYFNCDMVQAAHGRAPAVATGIKRVHPDDVVFTYQGDGDLAAIGT
                     AEIVHAANRGEKITTIFINNAIYGMTSGQMAPTTLIGQVTTTTPFGREPQLHGYPLNV
                     CEMLSTLKGAAYVERVSMHDIKNIKNAKKAIKKAFQVQMAGLGFSIVEVLSSCPTNWG
                     IDPVNALKWIEEKMIPQYPLGNFKGKDLEV"
     gene            complement(1978..>3003)
                     /locus_tag="PLG72_10730"
     CDS             complement(1978..>3003)
                     /locus_tag="PLG72_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOJ81311.1"
                     /translation="EVIAEAALRAGCRHYFGYTTTPQPEIAHYFAKKMPQYGGTFIQA
                     ESEVAAMNMVYGAASAGARVMTSSSSPGISLKQEAISYAACAELPAVIINIMRAGPGL
                     GGILASQGDYFQATKGGGHGDYRMVVLAPSGVQELYELVVEAFNIADKYRTLCMIMGD
                     GMIGQMMEAVEFKDKEEIYQADKSWAVTGTEMKRDHNIITSIYIDPEVLEKHNLKLQE
                     KYRKIAANETRVEVFNCDGADIIVAAYGTVARIIKNVIKTAEKDGIKVGLIRPITLWP
                     FPTAAFEKYAEVPQAFLSVEMSAGQMVEDVRLAVNGKRPVHFLGRMGGMIPTQKEIYE
                     KIREILK"
BASE COUNT          701 a          805 c          642 g          855 t
ORIGIN      
        1 cagataatac atagcgcatt gaatggctga taaacagaaa atttacagaa tatacaactt
       61 atataaaggt aaatattcca catcataaat acgaggcgat aatagatatc tcacgcctcg
      121 ctgaaacgtg ctggggtaag gaaaatttcc tgaaatcggg gatttttctt tgaatgatgc
      181 gttatatgga cgtgatatgg aggcctgtat gaaatcgcac atgcacaaat tcaaattcga
      241 ctgcagactg acaaacggcc ataaccacag gctgcttgga tatgcaagcg gaatggtggg
      301 catcggcagc ttccattttc acttttatta tggtgtttca tcctaccgcg accataccca
      361 ttatttctgc ggagtgactg gaatgcccca taagaccgaa aacgggcaca tacacaagat
      421 ggaaggcata ctggaataca atgactcgca cgaacatgtt tacaaagggt ataccttcga
      481 ggagatatca tatatccctt ccgccagcgt catcggtttt gtccgctgaa ccgcagatcc
      541 gcagtccctt cgccggaatg accgcactgc cagccattgg caatgacgca tttgccgtga
      601 tatgaaaatg acccgccaaa cgctggtctc gtttgcgggc ctctcattgt tcacttgctt
      661 acaggtacaa ataaattttg ctcaatattc cattcctatt ttcagcgctt tcatttcatc
      721 agggatcaga taatgctttt tttcaggcag tacctttttc agccctcgtt caaaattctc
      781 cggcgaaacg actcctgttt ccttaataag cttaccgagc atgattatgt ttgcaaaagc
      841 caggtttccc atatcagagg cgatttgtgt cgccggtata gcaaatgccc ttatgtcctt
      901 cctggccggt tccctgttta ccagtgagct gtcatacagc agtatcccgt ccggctcgat
      961 ataattctcg aacttgtcca tcgacggcct gttcattgcc attacaacag tcgcgctgtt
     1021 cagtatcggt gaacctatgg gttcgtcaga cacgatgaca tgacagttgg aagtgcctcc
     1081 gcgcatttcc gggccatagg aaggaaggaa ggaaacaaac ttgtcctcca ttatccctgc
     1141 gtatgccatc agttttccgg ctgcctggat cccctggccg ccaaatcctg caaaaatcat
     1201 atcagtctga gccatatgct acacctccag atcctttccc ttgaagttgc caagcggata
     1261 ttgcggtatc atcttctcct cgatccattt gagcgcgttt acaggatcga tcccccagtt
     1321 ggtcgggcat gaagaaagga cttccactat ggagaagccg agtcctgcca tctggacctg
     1381 gaatgctttt tttattgcct ttttggcatt ctttatattt ttgatatcat gcatggatac
     1441 acgctctaca taggctgctc ccttaagcgt ggaaagcatt tcgcacacat tcagcggata
     1501 tccgtggagt tgaggttccc tgccaaaagg tgtggtagta gtcacctgtc cgataagcgt
     1561 ggtcggggcc atctgtccag acgtcatacc atagatcgca ttgttgatga atatggtcgt
     1621 tatcttctct cccctgttgg cagcatggac gatttccgct gtaccgatag cagcaagatc
     1681 accgtcaccc tgataagtaa ataccacatc atcaggatgg acacgcttga tgccggttgc
     1741 tactgccggc gctcttccat gggcagcctg taccatatcg cagttgaaat acaagtaatt
     1801 gttatacgca catcccacag gggatacgcc tattgttttc ccctctatac caagctcgtc
     1861 gatcacttct gctatcagac ggtgcacgat gccatgcgtg cagcccggac agtaatgcag
     1921 ctgagtctct ctcaaggcat gaggcctctg aaatactata gccattgtca ttccccttta
     1981 cttcagaatt tccctgattt tttcgtatat ttccttctgg gtcggtatca tgccgcccat
     2041 ccttccaagg aagtgcacag gccttttgcc gttcaccgcc agtctgacat cctcgaccat
     2101 ctggccagca ctcatttcca cagaaaggaa tgcctgcggg acttccgcat atttttcgaa
     2161 agctgccgtc gggaaaggcc acaacgttat cggtctgatc agtccgacct ttattccgtc
     2221 tttttcggca gtttttatca cattcttaat tatccttgcc acagttccgt atgcagctac
     2281 gataatatca gctccatcgc agttgaaaac ttcgactctc gtctcattcg cagctatttt
     2341 cctgtatttt tcctgcagct tcaggttgtg cttttccagg acctcgggat ctatatatat
     2401 ggaagtgatt atattatgat cccttttcat ctccgtacct gtcactgccc aactcttgtc
     2461 tgcctgatat atctcttcct tgtccttgaa ctctacggct tccatcatct gtccgatcat
     2521 accgtcgccc attatcatgc acagggtgcg gtatttatct gctatattga aagcttccac
     2581 cacaagttca tacagttcct ggacgccgct gggcgcaagt accaccattc tgtagtcgcc
     2641 gtgtcctccg cctttcgtag cctggaaata gtcgccctga gatgcaagta tgccgccaag
     2701 tcccgggcct gctctcataa tatttatgat tacagccggc aattcggcgc aggccgcata
     2761 tgatattgct tcctgcttca ggcttatgcc cgggcttgag gacgatgtca tcactctcgc
     2821 ccccgcactg gctgcgccgt ataccatgtt catcgccgcc acttcactct cggcctgtat
     2881 gaaagtaccc ccgtactggg gcatcttttt tgcaaaataa tgagctatct ctggctgagg
     2941 tgttgtagtg taaccgaagt aatgcctgca gcccgctctc aatgcggctt cagcgatcac
     3001 ctc
//
LOCUS       DAOSCC010000257         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_21448_length_2971_cov_4.094307, whole genome shotgun sequence.
ACCESSION   DAOSCC010000257 DAOSCC010000000
VERSION     DAOSCC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21448_length_2971_cov_4.094307"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1617)
                     /gene="aspS"
                     /locus_tag="PLG72_10735"
     CDS             complement(292..1617)
                     /gene="aspS"
                     /locus_tag="PLG72_10735"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOJ81312.1"
                     /translation="MLTIKGELASAHVEAEELINHIGQTVQIHGIVYKIREMSGFAFV
                     LLRTKRALVQCIHSGDFSTFPLSELKENMSVIIRGEVVRDERSRTGTEIRIISFEALS
                     CPAEEMPVVINNREVNASIETLLDFRPITLRNIKERAIFKVQEGICAGFRSFLGENGF
                     TEIHTPKIVFSGAEGGANIFRLDYFGREAYLAQSPQFYKQIMVGVFERVYEIAPVFRA
                     EKHDTSRHLNEYTSVDLEMGFIKDFTDLMRMETKMLAYTFDYLKEHYADELAMLKAEI
                     PTADDIPAVSFVEAKQMISDAYHREITDYEDFDPEEEKLLSEIIKKQTGSDFVFVTGF
                     KSSKRPFYTQDSKDDPEYTDSFDLIFRGMEITTGGQRIHDYNVQVAKMRRLGMDVDKF
                     SSYLMAHKYGLPPHGGLGLGLERFTARLLGLQNVRYAAMFPRDINRLEP"
     gene            complement(2110..>2971)
                     /locus_tag="PLG72_10740"
     CDS             complement(2110..>2971)
                     /locus_tag="PLG72_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81313.1"
                     /translation="HAADPKECKLRNTFQQAIQFYPEVFYKLGNAADEICSADYALVI
                     SKSDFDDVMKSFSRSHVYKENGHRFRAILDLGLQMNEVGGKYCITSMNYPKMFLGLWV
                     LCNAPRSKYKYMNYLRLDYKGYYRAMPEIGDVRKTMKEEHAGYVDSILKSFKGQKIRY
                     KVKPLRNITSGFDWKVDFMLKGRNVFGFYAEPDYFKLCIYFSNTKNITKFGEKLKLFN
                     PELFDWYCGKFPERLCKCPNNRVVKFGDTKKRICGLTNRAEIANPNDHDIENSKTVIE
                     MILSPEKAAH"
BASE COUNT          740 a          750 c          647 g          834 t
ORIGIN      
        1 agtgacggta aggtgcgtcg tcccgctgcg caggcatgct tccgccgaca gcacgccgaa
       61 ataggacgga agagtccccg aagaagctga atgatttcga agatgtcatc ggaaatcgat
      121 gaatggtatc atcataatta agacagtgta tcagaaagcc atgctctgga aatccatgca
      181 acggaaaaac ctgcttgtat gcgttcaagc aggtttttct gcaaatcgta ctttcgtgtt
      241 cagtacaggc aaaatcccca atgccagggt aaacggcgat attccatgaa atcaaggttc
      301 caggcggttt atgtcccgcg ggaacatcgc agcgtacctg acattctgca gccccagaag
      361 ccgggctgtg aagcgttcta ggccgagacc cagcccgccg tgcggcggca gcccgtattt
      421 atgtgccatg aggtagcttg agaacttatc gacatccata ccgagcctgc gcatttttgc
      481 aacctgcacg ttgtaatcgt ggatcctctg cccgcctgtc gtgatctcca tgcccctgaa
      541 tatcaggtcg aaactgtcgg tatattccgg atcgtccttc gaatcctgcg tatagaacgg
      601 ccgcttggat gatttgaacc ctgttacgaa gacaaagtcg cttccggttt gttttttgat
      661 tatctcactc aggagctttt cttcctccgg gtcgaagtcc tcataatctg tgatttcacg
      721 gtgatatgca tccgatatca tctgttttgc ttcaacgaag ctgactgcgg ggatatcgtc
      781 agccgtgggg atctcagcct tgagcatcgc cagttcatcg gcgtaatgct ccttcaggta
      841 atcaaaagtg taagcaagca tcttcgtttc catacgcatc aagtcggtaa agtcctttat
      901 gaagcccatt tcaaggtcaa cgctcgtgta ctcgttcagg tggcgcgaag tgtcatgctt
      961 ttcagcccgg aacaccggcg cgatctcgta taccctctcg aaaacaccga ccatgatctg
     1021 tttatagaac tgcgggctct gggcaagata tgcttcccgg ccgaaataat ccagcctgaa
     1081 tatattagct cctccttcag ccccgctgaa aactattttt ggcgtatgga tctcggtaaa
     1141 gccattttcg cccaggaaac tcctgaatcc agcgcagatg ccctcctgga ccttgaaaat
     1201 cgcacgttct tttatgttgc ggagcgttat cgggcggaag tccagcaggg tctcaatgga
     1261 cgcatttacc tccctgttgt ttattaccac gggcatttct tcagcagggc aggacagtgc
     1321 ctcaaaactg atgatcctta tctctgtacc ggtcctgctc cgctcatccc tgaccacttc
     1381 gcccctgatg atgacgctca tgttctcttt cagttcgctt aaggggaatg tcgagaaatc
     1441 accggaatgg atacactgca ccagggctct tttggttctg agcaatacaa atgcaaagcc
     1501 ggacatttcg cggatcttgt atacgatccc atggatctgc actgtctggc ctatatggtt
     1561 tataagttct tccgcctcga catgggcgga agcgagttca ccctttatag tcaacatgta
     1621 taacatctcc ttatctcgaa attctgagcc aactcaaaat aacaaatcgt ttcaaacgtt
     1681 caaattcaat atttcaagca acatagcaaa tcccgtattt gggatcaggc aacagcagat
     1741 ccactgtcac acagatccat tccggcatgg tcagcatgcc gcatactacc acatcccttc
     1801 aaataaaaac accgcatcgg tatcgataat ccgtaccagt gcggtgatat gatgcaagta
     1861 taagggaaag accataccag cagccacaca ggtacagagc cgaaagcacc tgcacctgcg
     1921 gtctgcgtta acgccgactc ataggtgcaa gtgctgcata tcagtatcct ttttatggct
     1981 gaaataatgc agcatggtat ttccttcctt catggcattg atatatattt attataaata
     2041 atatcacaaa aatttgataa agcaatcttt ttcaggcgaa ccagctttaa gactgcgtca
     2101 tgactttgcc taatgcgcgg ctttttcagg agaaaggatc atttcaataa cagttttact
     2161 gttttctata tcatggtcgt tcggatttgc tatttccgcc ctgttggtca atccgcagat
     2221 cctttttttt gtatccccga attttacgac cctgttattc gggcacttgc acagcctttc
     2281 cgggaatttg ccgcaatacc agtcaaaaag ctctggattg aataatttga gtttttcgcc
     2341 gaatttcgtt atgttcttcg tattactgaa ataaatgcaa agcttgaaat aatcaggttc
     2401 ggcatagaag ccgaaaacat tcctgccttt cagcataaaa tcgaccttcc agtcaaagcc
     2461 tgacgttata tttctcaatg gtttgacttt gtacctgatc ttttgccctt tgaacgactt
     2521 aaggatcgaa tcgacatatc cggcatgttc ctctttcatg gtcttcctga cgtcgccgat
     2581 ttccggcatg gccctgtaat agcccttata gtccaggcga agataattca tatatttgta
     2641 cttgctcctg ggtgcattgc ataggaccca aagtcccaga aacatcttag gataattcat
     2701 tgacgtgatg caatatttac cgccaacttc gttcatttgc agccccaggt ccaggatcgc
     2761 cctgaagcgg tgaccgtttt ccttgtatac atgggatctg ctgaaggact tcataacatc
     2821 gtcaaagtcc gactttgata tcaccagtgc gtaatctgcg ctgcatattt cgtctgctgc
     2881 atttccaagc ttgtaaaaca cctcgggata aaactgtatg gcttgctgga aagtgttccg
     2941 gagcttgcat tcttttggat ctgcagcatg c
//
LOCUS       DAOSCC010000258         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_21560_length_2960_cov_4.461618, whole genome shotgun sequence.
ACCESSION   DAOSCC010000258 DAOSCC010000000
VERSION     DAOSCC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21560_length_2960_cov_4.461618"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..991
                     /locus_tag="PLG72_10745"
     CDS             407..991
                     /locus_tag="PLG72_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ81314.1"
                     /translation="MDERQLIDKIKRKPRDGLIDAFDSFGPMVKAITVKVLGKSSSED
                     VEECVSDTFVKLWQSIGSYDERKGSLKSFIAAIARNTAIDRYRKNRSGIASLPIDGLD
                     EASPQDTETAAIKRIDSDLVRDAVRDLAEPDREIFFRRFFLSETISDIANKMDLKNKR
                     VEKKTVQRQAKTARYADQQRDQLLKGMIICLTVT"
     gene            973..2424
                     /locus_tag="PLG72_10750"
     CDS             973..2424
                     /locus_tag="PLG72_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81315.1"
                     /translation="MFDGYMKDTEMDIVEEPISDIEAARIKKMIVKKVGRKQGTMKKR
                     MAAALAACCLMLTGVTAFAFTGLNIAEAFRAIFGEKASYLENIYPIEQGVSNAGLTVT
                     VRGAAGYDKVAFMLVEIARDDGKPFIGDNLETAVTSLDVEGLSDYSVGSSGVSVVDGK
                     AVITFNLQSTEELKGRNVSINIGDICYRELKFEKASLDLLDDLGLPQADAEDLVENTA
                     KTAGYVSRYEPRSTRKPFFAEYVRNAALIPEKIAASQDLGLPLLAGKPGLLLDTASFV
                     DGKLHVRVLNMSAGYDRDEVLLYMMDPLTGGIAPMMVWNEPAEDMNAMYYVYDISDVS
                     QLEQYEPMVEYYGEYETFEGSWNVKFVLDNDAEGPDFRPAVDMQGYDDSLVLDEIYVT
                     PTATYVRFLPKDSQKVEVGLSLTADIRMKDGSYIHYNNCSCSSLNQEYLEFTGFYDPI
                     DVSDIDAITVKSWDQQINDQTDRWYNETFRYID"
BASE COUNT          841 a          609 c          829 g          681 t
ORIGIN      
        1 cgagcctcga ccgcaggaga gcgggcagga ctgcaccggc ccacggcctg tatctagccg
       61 aagtattcta ctgatgattt tatgacggac agtttccgga acggaggttt attgcttttc
      121 cggcggaagt gtttccggtt ttgaaatttc agttttaccg ggtatgaagt ttcctgacgg
      181 atccggaact ggagattcca tgtgaaagtc cgggataagc agcaattgga tcgtgataaa
      241 atggcccggg cagattatcg agagatccgg ataaaccatc gataaatttc aggcggaaag
      301 ttgcctgaaa ttttctttat accagggggg gacctgtgag ttcccgacgt atatacatat
      361 gaaggccttc ggcggtatat cttttcactg catgggagag gagccaatgg atgaaagaca
      421 attgatcgat aaaatcaaac ggaaacccag ggacgggctg atcgacgcat ttgattcatt
      481 cggcccgatg gtgaaggcca tcactgttaa ggtccttgga aaaagcagtt ccgaagatgt
      541 tgaggagtgc gtatcggata cgttcgtcaa gctgtggcag tcgatcggat catatgatga
      601 gcggaagggg agcctgaaat cctttattgc cgcgatagcg cgcaacacgg caatagacag
      661 gtacagaaag aacaggtcgg gaatcgcgtc tctcccgatc gacggcctgg acgaagcttc
      721 tccccaggat acggaaactg cagctattaa aaggatcgac agtgatctgg tcagggacgc
      781 tgtaagagat ttggcggaac ctgacaggga gatttttttc aggaggtttt tcctgtcgga
      841 aacgatcagc gacatagcca acaagatgga tcttaaaaac aagcgggtcg aaaaaaagac
      901 tgtacaaagg caggcaaaaa ctgcgcgata tgctgatcaa cagagggatc aattactgaa
      961 ggggatgatc atatgtttga cggttacatg aaggatactg aaatggatat tgtagaagag
     1021 cctataagcg atatcgaagc tgcacgcatc aaaaaaatga tcgtgaaaaa agttggccga
     1081 aagcagggca ccatgaagaa aaggatggct gcggctcttg cggcatgctg ccttatgctg
     1141 accggtgtga cggcatttgc ttttacagga ctgaatattg ccgaggcgtt tcgggcgatt
     1201 ttcggagaaa aggcatccta ccttgaaaat atatacccta tcgaacaggg cgtttcgaac
     1261 gcaggactga ctgttacagt ccgaggagcg gcaggatacg acaaggtcgc gttcatgctg
     1321 gtggaaatcg cccgggacga cggaaaacca ttcattggcg ataatctgga aaccgcggtc
     1381 acgtccctgg acgttgaagg gctttccgat tacagtgtcg gttcatccgg cgtaagtgtc
     1441 gttgacggca aggccgtaat tacattcaac ctgcagtcga cggaggagct gaaaggcagg
     1501 aatgtctcca taaatatcgg tgatatctgt tacagggaac tgaaatttga aaaagcatcg
     1561 cttgatttat tggacgacct gggacttccg caggctgatg ctgaggacct ggtcgaaaac
     1621 acagcaaaga cagcgggata tgtaagcaga tatgaaccca ggagcacaag gaagccattc
     1681 tttgcagaat acgtgaggaa tgcggcgctg atccccgaaa aaatcgcagc aagccaggat
     1741 ctcggtctgc cgctgctggc ggggaaaccg ggattgctgc tggatacggc atcctttgtc
     1801 gacggcaaac tccatgtccg ggtattgaat atgtcagccg gctatgaccg cgatgaggtc
     1861 cttttgtaca tgatggatcc tctgaccggc ggcatcgctc cgatgatggt ctggaacgag
     1921 cctgctgaag acatgaatgc gatgtattat gtttatgaca tttcggatgt ttcgcaactg
     1981 gaacagtatg agccgatggt cgagtattat ggggaatatg agactttcga gggcagctgg
     2041 aacgtgaaat tcgtcctgga caatgatgcg gaaggacctg atttccggcc ggccgttgac
     2101 atgcagggct acgatgacag ccttgtcctg gatgagatct atgtaacgcc aactgccact
     2161 tatgtcaggt tcctgcccaa agacagccaa aaagtcgaag tgggtctatc cctgacggca
     2221 gatataagga tgaaagatgg cagttatata cattacaaca actgctcctg ttcttcgctc
     2281 aaccaggagt acctggaatt tacaggcttc tatgatccta tcgatgtaag tgatatagat
     2341 gcgataaccg taaaaagctg ggaccagcaa ataaatgatc agacagacag atggtataat
     2401 gaaacgttca ggtatatcga ctgaaaatgt tttgtccagg ttcggcgaga acagttaccg
     2461 cacaaatttc cgggtgtatt tgccgcacgg atccattgaa cttgcagcga ggcagtgtaa
     2521 atagctttta ctctgaaaaa ctgctgaagt gagggaacat gctcacaatc gaaaagtcgc
     2581 tcacggaccg gtgatccatt acaggagcac tcagcggtac cgttacagga ccagggccag
     2641 ccctggtcct tttttacggc taattttgcg atatgcaggt gaatattaac gtaatttttg
     2701 atacaattgc agggcatgct gaagcgtttg tgacgatcgc cgcattaaac aaagatggca
     2761 ggaatgaaga caactacaga cagtgaagat aggacagaag agagttttcg gactgggaca
     2821 atggtatttt ggaatggttc caaaccgggg aaatgggacc gggacgcagg atctgacatg
     2881 aagaaccgga tccggagcaa gtctgaaaaa gtgaaatgac ggacgattat aagggagata
     2941 tgggtatatt aaggctataa
//
LOCUS       DAOSCC010000259         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_21751_length_2939_cov_3.930652, whole genome shotgun sequence.
ACCESSION   DAOSCC010000259 DAOSCC010000000
VERSION     DAOSCC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_2939_cov_3.930652"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLG72_10755"
     CDS             complement(<1..196)
                     /locus_tag="PLG72_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOJ81316.1"
                     /translation="MRYEILKLLKEAGSDYITGVDIGSRFGISRSAVWKHIKELRNEG
                     YSIESSPKLGYRLLADSGHLN"
     gene            complement(193..816)
                     /locus_tag="PLG72_10760"
     CDS             complement(193..816)
                     /locus_tag="PLG72_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81317.1"
                     /translation="MVEVYFYMPAGRAGDAVDCGIKLSEWYSRELCIDGTWRKCITAL
                     LNPRDEYEKFISSEYKCLKLEIPPKYCYVADRSLYEAGRRNPGAMRLYEESIIPVMSY
                     RLGDYRFPEALVTSTVLGEQVSVTGKMLDTPVLYTNSRELYLGNLTAQLREEHSDLDD
                     TFLYLYFKWLCSQGEADMVCDGADSPAVFILKESKKPYIFNAPGRTQ"
     gene            1129..1614
                     /locus_tag="PLG72_10765"
     CDS             1129..1614
                     /locus_tag="PLG72_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ81318.1"
                     /translation="MSFLENLGKKVGEAAQAAAKKSSELVEVTKLNANINSEEDKIQK
                     LYAQIGKTIFEKFEQTGTAEDIVKEACEQIVAHKENIKALREKIAEVKGIKQCINCGA
                     EMERTQLFCAKCGTRNDLPQQPAETQPAEQAAAPAPTCPSCNAELAPGSLFCTNCGTK
                     L"
     gene            complement(1812..>2939)
                     /locus_tag="PLG72_10770"
     CDS             complement(1812..>2939)
                     /locus_tag="PLG72_10770"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOJ81319.1"
                     /translation="TGGIFIGDECIQKGDNTGALSAARVLGDTRVEAAVLETARGGII
                     KRGLGYDLADVGVIINISDDHLGLDGINTKEELAKVKSLVAEAVKPEGTAVLNADDEM
                     TPFILGRINCRPLLFSSDSGSLMIRKWMRDGRDAVYVRDGSIYAFVSGIEKHIVRISD
                     IPITFGGKAACNIENSLAAVSALLALGISAAVIRKGIMSFKPDLDVNPGRFNIFDMGE
                     FSVMLDYGHNKAGYSAVIDMIKSFNAPIYTGVIGMPGDRRDESIIEVGRICGSFFSKL
                     YIKEDSDLRGRDAGEVADLLYSGAIEAGMDKDRISVIYQEDLALKRAIMDAVPGEMIV
                     MFYEELDSAVEVIEKYRKSMGLAGPGAGKAGKPGIHRSATG"
BASE COUNT          692 a          828 c          677 g          742 t
ORIGIN      
        1 gattcagatg cccgctgtcc gccagcagcc tgtagcccag tttcggagac gattctatgc
       61 tatatccttc attcctgagt tcttttatgt gcttccagac ggcagacctt gaaatgccga
      121 aacgcgatcc tatgtcgact cccgtaatat aatcggaacc agcttccttt aaaagtttca
      181 gaatctcata tctcattggg tcctcccagg tgcattgaat atgtatggct ttttgctctc
      241 tttcagtatg aatacggcag gactgtcagc gccgtcgcat accatgtcgg cttccccctg
      301 gctgcataac catttgaagt acaggtaaag gaatgtgtca tccaggtcgc tgtgctcttc
      361 cctgagctgc gcggtcaggt tgccgaggta aagctcccgc gaattggtgt acaataccgg
      421 cgtgtcgagc attttgccag tcacgcttac ctgttcgccc agcacagtgc tggtgacaag
      481 cgcttcaggg aacctgtagt cgcctaatct gtaagacatg accggtatta tggattcctc
      541 atataacctc atagctccgg gattcctgcg gccggcttcg taaagcgatc tgtcggccac
      601 atagcagtat ttcggaggta tctcgagctt aaggcacttg tactcgctgg atatgaattt
      661 ttcgtactcg tcccttgggt tcagcagtgc agtgatacat ttcctccagg tcccgtctat
      721 acatagctca cggctgtacc actcagagag ttttatcccg cagtctacag cgtcgccggc
      781 cctgcctgca ggcatataaa aatatacctc taccacacta tcacctccgt gacgatgcgg
      841 agccatcctg catcgggatc atcccgctta acgtatccca acttttattc tactgtaacg
      901 ctgctgtctg ttcaaccatt tatccacagc ccctggccat taaccgtgcc tgtgcaggca
      961 cgggcgccga cgcatgcggc tgtctccagc ctgtcttccc tattgcctga atggcgggaa
     1021 atgtcatctt gtattgaatg tagcgctgat attacttata atagtcttat acaggaccag
     1081 ttttgtacaa aacatgatga aatcgtaatt ttgaatggag gtatcataat gtcattcctg
     1141 gaaaacctgg gcaaaaaagt gggtgaagcc gcacaggcag ccgcgaagaa atccagcgaa
     1201 ctcgtcgagg tcaccaagct caacgcaaac atcaactccg aagaagacaa gatccagaag
     1261 ctttatgcac agatcggaaa gacgattttc gagaaattcg aacagaccgg tacagcagaa
     1321 gacatagtaa aggaagcctg cgagcagatc gttgctcata aggagaatat caaggctctc
     1381 agggaaaaga tcgccgaggt caaaggcata aaacaatgca tcaattgcgg agccgaaatg
     1441 gaaaggaccc agttgttctg cgccaaatgc ggaaccagga acgatttgcc ccagcagcct
     1501 gctgaaaccc agccggcaga acaggccgct gctccggccc cgacctgtcc ctcctgcaac
     1561 gcagaactgg ctcccggttc attattctgc accaattgcg gtacaaagct gtgatgcggt
     1621 gacaaataca taacttatcc catatcaaaa gggcggtgtt tcatccgccc tttattaagg
     1681 tatggggata ctcctcgaca aggtatgcgt gatattcctc aaccatagag gaataattct
     1741 ttgccgagga gcgtcgccat acctttttct gcgatgaatg tccccttttc tgtggggacg
     1801 gtccatgatc cttaccccgt tgccgaccgg tgtatcccag gcttgcctgc cttcccggcg
     1861 cccgggccgg caagtcccat ggacttccga tatttctcta ttacttcgac tgcagaatcg
     1921 agttcttcat aaaacataac gatcatttca cccggcacgg catccatgat ggctctttta
     1981 agcgccaggt cctcctggta tatgacggag atcctgtcct tatccatacc cgcctctatg
     2041 gcgccgctgt acagaaggtc ggctacctcc ccggcatctc tccccctgag atcgctgtct
     2101 tccttgatgt aaagcttgga gaaaaagctg ccgcagatcc gtccgacttc aatgatgctc
     2161 tcatccctcc tgtcgccggg catgccaatc acacctgtat atatgggggc gttgaaactc
     2221 tttatcatat cgatgactgc gctgtaaccc gccttattat ggccgtaatc gagcatgacg
     2281 gaaaattctc ccatgtcaaa gatgttgaac ctgccgggat tcacatccag atcgggtttg
     2341 aaactcatta tgcccttcct gataacggca gcggatatgc caagcgccag aagcgccgac
     2401 acggcagcaa gcgaattctc tatattgcac gctgccttgc ctccaaaagt gatcgggata
     2461 tcgctgatcc gtacaatatg tttctcgatc cctgaaacaa aagcatatat ggaaccgtcc
     2521 ctgacatata ccgcatcacg gccgtccctc atccatttcc ggatcatgag actgccgctg
     2581 tccgacgaaa acagcagagg cctgcaattg atcctgccga gtatgaaagg cgtcatttca
     2641 tcgtccgcgt tcagtacggc ggtcccttcg ggtttgacag cttcagcgac cagcgatttg
     2701 acctttgcca gttcctcttt cgtgtttatg ccgtcaagcc cgagatggtc gtcacttata
     2761 ttgatgatga cccctacatc ggcaaggtca tagccaaggc ctctttttat gatgcctccc
     2821 cgtgcggtct caagcactgc ggcttccacc cttgtgtcgc ccagcacccg cgcagcgctc
     2881 aaagcgccgg tattgtctcc cttctggatg cattcgtctc caatgaatat gcctcccgt
//
LOCUS       DAOSCC010000260         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_22009_length_2916_cov_3.055925, whole genome shotgun sequence.
ACCESSION   DAOSCC010000260 DAOSCC010000000
VERSION     DAOSCC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22009_length_2916_cov_3.055925"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /locus_tag="PLG72_10775"
     CDS             <1..2182
                     /locus_tag="PLG72_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81320.1"
                     /translation="MPGHSSPGLLNNPSDGTERHNSNGLLLVSKASIAIKEGQATKNP
                     NAAKLHTYPGIVYALPGSTVEFSVLATDESYLPTAVPQQITWASSAGKIDSQGRLTVG
                     NNPGKYAVLAVSGLIAGASEIVIPDKITSLKPSKSVITLLPGDVIDLSCEAYYQNMKV
                     VSTDSSFTWEVEGNIGTITPEGVFTMSKDAQGSGRIRVSYGNTSAYINVIVPATPNAI
                     EDFEGAFGWGYTTVRAKSANVSVVQDPSLARSGSGVLKMDYDFTLGNGVEKGVAGVYA
                     YTLNPNTKTKTELSLNGNPVAIGMWVYGDNSKSWLRASVRDGSGQSFYIDFTPDYNPN
                     TGTGGIDWTGWRYVEAKIPSGRKGPFVLETPIRVMCSRDEMRTKGTLYFDQLRAVYSG
                     EEAVQETVVKITSPADNAVINTGRIPLTAEIITDPKGTGVDPNSIQVLLDGVVLSGLT
                     ITGNSNISIKGELGTELPLADGYHTLVVSYTDFSGKNGSKAITFTVDTGAPRVIATTT
                     PTVTEGGSFTTALEIKNPKTLRKIYADIIYDTNAVEVVDADSKTAGLQVALEPWVSKG
                     KVITHRVDATNGRITLEIDNLTNLSAEATAKLGTITFKAKPGFQDNTQIKLRLGAMIV
                     GSNPSSQRFNLPDMNVTMEYALSLKVEGIRPGETTVITVTDKSGNPVENADIYLNDIS
                     YPFWKTDKNGQVVTNLIAPMLEREPLKIRAKKDGQISKAIILGQ"
     gene            2257..2424
                     /locus_tag="PLG72_10780"
     CDS             2257..2424
                     /locus_tag="PLG72_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81321.1"
                     /translation="MRKSNFEAEIYRLVILAALVAIIGDIIDLIIACKQYCYALKGKT
                     DDAIEIEPFIL"
     gene            complement(2432..>2916)
                     /locus_tag="PLG72_10785"
     CDS             complement(2432..>2916)
                     /locus_tag="PLG72_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HOJ81322.1"
                     /translation="DITVPFHMSTVEFFKIVKDHLKPGGVLIVNVNMKSGSFTGVPEY
                     LANTVKSLFNKVYRADLSVVTNSLLFASDDNDMIENYKNNTANQIPLDHDLYNISRYI
                     SSHLVEITDTKLVLTDDLAPVEILGQKALSEIVQSEVERVSGMLKGKSLQEIFEYLAN
                     "
BASE COUNT          844 a          679 c          722 g          671 t
ORIGIN      
        1 aatgcccgga cacagcagcc ccgggcttct gaacaaccct tcggacggta cagaaagaca
       61 taattccaac ggacttttgc tggtttcaaa agccagtatt gcaataaaag aaggacaggc
      121 aaccaaaaat cccaacgcgg caaagctgca cacatatccg ggtattgtct atgcacttcc
      181 tggctccact gtagagtttt cagttctggc gaccgatgaa agctatctgc ccacagctgt
      241 gccacagcag ataacatggg ccagcagcgc cgggaagata gactctcagg gcaggctcac
      301 agttggaaac aatcctggca aatatgcggt tctggctgtc agcggcctca ttgcaggtgc
      361 ctcggagata gtgatacctg ataaaataac ttcattaaaa ccgtccaaaa gcgttataac
      421 tcttcttccg ggggatgtca tcgacttaag ctgcgaagcc tattaccaga acatgaaagt
      481 tgtaagcaca gacagctcct ttacctggga ggtcgaagga aatatcggga ctatcacccc
      541 tgaaggagtc ttcaccatgt cgaaggatgc ccagggaagc ggacgtatca gggtcagcta
      601 tggaaacacc agcgcatata taaatgtgat agttcctgcc acaccgaatg ctatcgagga
      661 tttcgaaggc gcattcggct ggggatacac tacggtcagg gctaagtctg ccaatgtatc
      721 tgtcgttcag gatccttcac tggcaagatc gggcagcggc gttctgaaga tggattatga
      781 ttttaccctt ggaaacggcg ttgaaaaagg tgttgcagga gtttacgcat atacactgaa
      841 tccaaatacc aaaacaaaga ccgagttatc cttgaatgga aatcctgtcg ccataggcat
      901 gtgggtctac ggtgacaaca gcaagtcgtg gctgcgtgca agcgtccgtg atggcagcgg
      961 ccagagcttc tatatcgatt tcacacctga ctacaatccg aacaccggaa caggcggaat
     1021 agactggacg ggctggagat atgtcgaagc gaagatacct tcaggcagga aagggccgtt
     1081 cgttctagag acacctataa gagtgatgtg ttcaagagac gaaatgagaa cgaagggaac
     1141 actgtacttt gaccagttaa gagccgtcta ctcaggcgaa gaagctgttc aggagacagt
     1201 agtcaaaata acctcgcctg ctgacaatgc tgttatcaat accggcagga taccgctgac
     1261 ggcagaaatc atcactgatc ccaaaggaac cggcgtcgat ccgaacagca tacaggtgct
     1321 gctcgacgga gtcgttttgt cgggactgac cattaccggc aacagtaata tatcgatcaa
     1381 aggtgagctg ggtaccgagc tgcctctggc cgatggctac cacacccttg tggtcagcta
     1441 caccgacttc tccggcaaga acgggtcgaa agcaattacc tttacggtag atacgggagc
     1501 gccgagggtc atcgcgacca cgacgcccac tgtgacagag ggaggcagct tcacgaccgc
     1561 gctggagatc aagaacccga aaacattgag aaaaatatat gcagatatca tatatgacac
     1621 aaatgctgtt gaagtagtgg atgccgacag caaaacagca ggtcttcagg tggcgctgga
     1681 gccctgggta tcgaagggca aggtcattac tcatagagtg gatgcgacca acggacgcat
     1741 tacacttgag atagacaatc tgaccaatct gtcggctgaa gccactgcta agcttggaac
     1801 cataacattt aaggcaaagc ccggattcca ggataatacc cagatcaagc ttcgtctggg
     1861 agccatgata gtaggaagca atccctccag ccagaggttc aacctgcctg atatgaacgt
     1921 gaccatggaa tatgcgctgt cgcttaaggt tgaagggatc aggccgggag agaccactgt
     1981 cataactgtt accgacaaga gcggaaatcc tgtagaaaac gccgacatct atctcaatga
     2041 tatatcatat cctttctgga agaccgataa gaacggacag gtggtcacca accttatcgc
     2101 tcccatgctt gaacgtgagc ctctgaagat cagagccaaa aaggatgggc agatcagtaa
     2161 agctataatt ttgggacaat agcgaaggac ataatagaac cggggataag tctttggaat
     2221 agtatatcag tacaaagact ttccccgggg agtgttatgc gcaaaagcaa ttttgaggcc
     2281 gaaatataca ggctggtcat tttagccgcg ctggtagcga taataggcga tataatcgat
     2341 ctgattatcg catgcaagca atattgctat gctctgaaag gcaagaccga cgatgccatc
     2401 gagatagagc ctttcattct ttgatcatca ttcagtttgc caaatactca aatatctcct
     2461 gaaggctttt ccccttcagc ataccgctga ctctttcgac ttccgactga actatctcac
     2521 tcaatgcttt ctgaccaagg atttcaacag gcgcgagatc gtctgtcaaa acaagttttg
     2581 tatcggtgat ttcaacgaga tgactgctga tatatcggga aatattatac aagtcatgat
     2641 ccagagggat ctggtttgca gtattgtttt tataattttc gatcatatca ttgtcatcgc
     2701 ttgcaaaaag cagcgagttg gtgacaaccg acagatcggc cctgtatacc ttattgaaaa
     2761 ggctttttac ggtgttcgca agatattcgg ggacacctgt aaagcttcct gatttcatat
     2821 tgacattcac gatcagtact ccgccgggct tcagatgatc ttttactatc ttaaaaaatt
     2881 ccacggtgga catgtggaaa ggcacggtaa tatcct
//
LOCUS       DAOSCC010000261         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_22062_length_2910_cov_5.684764, whole genome shotgun sequence.
ACCESSION   DAOSCC010000261 DAOSCC010000000
VERSION     DAOSCC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22062_length_2910_cov_5.684764"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..276
                     /gene="rrf"
                     /locus_tag="PLG72_10790"
     rRNA            160..276
                     /gene="rrf"
                     /locus_tag="PLG72_10790"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            283..358
                     /locus_tag="PLG72_10795"
     tRNA            283..358
                     /locus_tag="PLG72_10795"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Asn,seq:gtt)
     gene            complement(449..1186)
                     /locus_tag="PLG72_10800"
     CDS             complement(449..1186)
                     /locus_tag="PLG72_10800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ81323.1"
                     /translation="MINVRGKWAFITGAARGIGYLASISMARQGCNLVLHSRSREHTK
                     KVLSEVRALGVEAYDVEAELTDAAAIGKMLDEIDKKGTQVDIVLNNAGLQPGYRTDYF
                     ATPVSDFTSAFWVNTIAPAMICYRFLPGMIERGFGRIVNTTSGIRNEPQQAGYSAAKA
                     ALDKFTTDLAATVDGTDVIISLTDPGWCRTDMGGQHAPNSPESALPGVIVGVFVNDRK
                     SGRLFRAQEFTGMTLEEAVIKAEKANA"
     gene            1750..2640
                     /locus_tag="PLG72_10805"
     CDS             1750..2640
                     /locus_tag="PLG72_10805"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOJ81324.1"
                     /translation="MDIQIITAPVVGGLIGLITNGLAIRMMFRPLKPIYIGRFRLPFT
                     PGLIPKERERLAKSIGEVISRELINKDTLKTTLLSGSMKEQFSAKLDEIIDKYRNSGD
                     TVGSIAEKFFEKEKMQEKLDNAKDVLAQTITKRAIEQDIGRTIVDYAYDEIMSKTKPI
                     LKSITASALNSMKKPFANRINDLIASRSKPLIEKFLDGEADELINMPLKDIITKYQDR
                     LPEVKEYLWNVYTDIITKKLEGVLDTVGIAEVVSNKINDFELLELENLVTTLMRKELN
                     ALIWLGGLLGLIMGFVNVII"
BASE COUNT          798 a          664 c          723 g          725 t
ORIGIN      
        1 agtagaacat catgttttcc ggtcgctgag cattatatag ttttgagggt gcaaagggag
       61 aaaggcccgg tgacactcct gcgtaacagg tattccttta tggctgagga atgtcacgca
      121 tgtggataag accggttgca cttgaaaagt ttatatgttt ctggtggaaa taacgagaag
      181 gacacacccg ttcccatccc gaacacggaa gttaagcttc tcagtgccga cgatacttgg
      241 ctggagacgg cctgggaaaa taggtctccg ccagaattat attcctcaat agctcagtcg
      301 gtagagcatg cggctgttaa ccgcagggtc gtaggttcga gtcctacttg aggagccacg
      361 caaaaacccc gctcaaaagg cggggttttt gggttgaagg tgcggtgaca ttcctctgca
      421 agaggtattc ctttatggtt gaggaatgtc acgcgtttgc tttctccgct tttattacgg
      481 cttcctcaag cgtcatgccc gtgaactcct gtgccctgaa cagccttccg cttttcctgt
      541 cgttgacgaa aacgcccacg atcacgcccg gcaatgcgct ttctggcgag ttgggcgcat
      601 gctggccgcc catatcggtc ctgcaccatc ccgggtcggt aaggctgatg attacatcag
      661 tcccatccac tgttgcggcg agatccgtgg tgaatttgtc aagtgccgct tttgcggcgg
      721 aataaccggc ctgctgcggt tcgttgcgga taccgctggt agtgttgacg atccggccga
      781 aaccgcgttc tatcatcccg ggcaggaatc tgtagcagat catggcaggc gcgatggtgt
      841 tgacccaaaa tgctgacgtg aaatccgaaa caggcgtagc gaaatagtct gtgcgatagc
      901 ccggctgtaa accggcatta ttcagcacga tgtctacctg ggtgcctttt ttgtctatct
      961 cgtcgagcat tttcccaatg gctgcagcgt cagtcaattc tgcttccaca tcatatgctt
     1021 cgacgcccag cgcccttact tcggaaagca cttttttagt atgctctctt gacctgctgt
     1081 gcaataccag gttgcatccc tgccgtgcca tggatatcga ggcgagataa ccgatgcctc
     1141 ttgcagctcc ggtaataaat gcccattttc cccttacgtt gatcatattg gtctcctttc
     1201 agttaaataa ggcattgcag ccatgcgccg gagtgttccg gcagaaaatg tgctgctgca
     1261 tgcggccgtt agaagaaatc gtcgtccgcc aatctctata ataccacaaa tatgtggaaa
     1321 cttaacggcc gtataaggac ccatccttcc ggagaaatgc cgtatacccc agtctacata
     1381 aagcctccac gtttgactta catagggctt ctatacacgg cggaccggtt aagtagtcga
     1441 aatatagaga ttcgcggtaa aaatagccga aaccgccaaa atgaaactaa tatccgcagt
     1501 aaaagaactt ttttgcctgg actggcacaa gacaaaaacc gtactgtttc gttaatatta
     1561 atgggtgtac ggcatatttg actactgcat gttaagtaca ggctgctccg aaggttgccg
     1621 gccaggcccg gaagttgccg gtgccggcat ccgcaagcgc actgatagct tttacagcag
     1681 caactataca gaaaaaacga tacaataata ctgatacaag aaccaaattg ataaaagaaa
     1741 ggaaatcata tggacataca gataattacg gccccggtcg tcggcgggct tatagggctg
     1801 ataacaaacg gactggcgat caggatgatg ttccggccct taaaacccat atatatagga
     1861 aggttcaggc tgccgttcac tccgggcctg atcccgaaag agagggaaag gctcgcgaaa
     1921 tccataggcg aagtgataag cagggaactg atcaacaagg acactttaaa gacgactttg
     1981 ctgtccggat cgatgaagga gcaattcagc gcaaagctgg acgagatcat cgacaaatac
     2041 aggaattccg gcgatacggt cggatcgata gcagaaaagt tctttgaaaa ggagaaaatg
     2101 caggaaaagc tggacaatgc aaaggatgtc ctggcacaga caataacgaa gcgggccatt
     2161 gaacaggata taggcaggac catagtcgac tacgcgtatg acgagatcat gtcaaagacg
     2221 aagcctatac tcaaatcgat aaccgccagc gcgctcaact ccatgaaaaa gccttttgca
     2281 aacaggataa acgatctgat cgccagcagg tcgaagcctc ttatagaaaa gttcctcgac
     2341 ggtgaagcag atgagttaat caacatgccg ctgaaagata tcataaccaa ataccaggac
     2401 aggctgcctg aagtaaaaga gtatttatgg aatgtatata cggacataat cacaaaaaag
     2461 cttgagggtg tcctggatac ggtgggcatt gcggaagtgg tcagcaacaa gatcaacgat
     2521 tttgagcttt tggagcttga aaaccttgtc acgacattga tgaggaagga actgaacgcc
     2581 ctgatatggc tgggcggact gctgggtttg ataatgggat ttgtcaatgt gattatatga
     2641 ggatatgata cattctgaag atgcatttgc ctgactggca atagtattac gattacttat
     2701 acggcgctcc gcatcacagc gcatcagtat tatttttcgt atcgattttt catgatatcc
     2761 ggcttcccat aatataagtc ccacaatgaa ctgtaatgca gaagaatgcg gtacattcgg
     2821 ctaaaccgtg tttgcttctc attttattta ccttttttta atgggtttgt gttataatat
     2881 gtggtattat gtaataactt agaatatagt
//
LOCUS       DAOSCC010000262         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_22203_length_2897_cov_3.941942, whole genome shotgun sequence.
ACCESSION   DAOSCC010000262 DAOSCC010000000
VERSION     DAOSCC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22203_length_2897_cov_3.941942"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLG72_10810"
     CDS             complement(<1..757)
                     /locus_tag="PLG72_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOJ81325.1"
                     /translation="METGFDAGRPVRVSSFNGIDIYRVNTDKFKTSSIHFFFQDNLTR
                     ENVTKNALLPAVMRRGSMSYPTQRDISKELESLYGASFDCGVTKKGERHIIHFYTEFL
                     SKQYVPDGTDTFAAALSLLFDIIARPALVNGRFSDEYLAQEKDNLRIRIEGRVNDKMS
                     YSVDRCMEEMCRNEPFEIYDYGFVEDLAGITADELTAHYRTMLETFPMQVYLVGSITD
                     GEAEKVSELLSGLERGNTVALRNGFARKEKADIR"
     gene            1117..1293
                     /locus_tag="PLG72_10815"
     CDS             1117..1293
                     /locus_tag="PLG72_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81326.1"
                     /translation="MMRYTPNRKYPITHMKRNDQQVNSERIIDISIGTAAGVLAFVFL
                     NGMFWGYMIRKFRS"
     gene            1428..2222
                     /locus_tag="PLG72_10820"
     CDS             1428..2222
                     /locus_tag="PLG72_10820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ81327.1"
                     /translation="MPETVLYFLRSKKGSESLVFWSVVLLAACVPAGLLLRTGITVFA
                     TPLLRLGSYILMLRYLYIVLLKDFIAEYEAINRKLYSLGKSIEIKVKKRMREVERVNR
                     KLLDKSKTDPMTGLLNKAALLDAVERLISNKPEKEHSILMFDIDNFKEINDNHGHIAG
                     DKCIKALAAATGSNFRSFDITGRYGGDEFIAVLPDTGARQAFAIAERFRKVVDKTSSP
                     HLTISIGISSYPADGSDARSLIEAADKGLYISKAKGRNAVTYRDVY"
     gene            complement(2216..>2897)
                     /locus_tag="PLG72_10825"
     CDS             complement(2216..>2897)
                     /locus_tag="PLG72_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81328.1"
                     /translation="TEGEIGRYAVKKQYHRESALSALTDGIRYCRTSASIRELLAADL
                     VIGFCIISVNLAFYPFAFDILKVTAKGWGMMITAYYGANLIAMFLAEQMDGRTDKGGG
                     RIFYACLASAALIWLLYAVSKAYAVVLLLQFAEGTIIAVSGIILAARCQTAADREYMA
                     RVAAFSDMMAGIGKLAGMSAASFIIRKCSFIGVFAFCGVLMFIFAIKGSTGSVWGRGR
                     KPSCTAGQ"
BASE COUNT          769 a          750 c          642 g          736 t
ORIGIN      
        1 gcctgatgtc cgccttttcc tttctggcaa aaccgttccg cagcgctaca gtgtttccgc
       61 gttcaagtcc tgacaacagt tccgacactt tttcagcttc tccgtctgtt atgctgccta
      121 ccagatagac ctgcatcggg aaagtctcaa gcatcgtccg gtaatgcgcc gtcagttcat
      181 cggcagtaat tccggcaaga tcctccacaa acccatagtc gtatatctca aagggttcat
      241 tgcggcacat ctcctccata cacctgtcga cgctgtaact catcttgtcg ttgaccctgc
      301 cctcaatcct tatcctcagg ttgtctttct cctgggcaag gtattcatcg ctgaacctgc
      361 cgtttaccag tgcaggcctc gcaatgatat cgaagagcag tgacaatgcc gcggcaaaag
      421 tgtcagttcc gtccggaaca tactgctttg acaggaattc cgtgtaaaag tggatgatat
      481 gcctctcgcc tttttttgtc acaccgcagt cgaagcttgc gccataaagg ctttcaagtt
      541 ccttcgatat gtcccgctgg gtcgggtatg acatgctccc gcgcctcatg accgcaggca
      601 gcagtgcatt ttttgtcacg ttctctcttg tgagattgtc ctggaaaaag aaatgtatgc
      661 tgctagtttt gaatttgtcc gtatttaccc tgtaaatatc gatcccgttg aatgaggaaa
      721 ccctgaccgg ccttcccgca tcgaatcctg tctccatcag aaccttcctt cccactaagt
      781 tcttttatat attataacac ctttaatcaa agaaaatttt tcgttcgtag aaaaattttc
      841 tttcatcagc ggtgtttcaa cgagacacca tgccttaagg tctcgttata acgcatgaga
      901 gaaaattttc ggttgtagaa aattttctta cctctgtgcg tttcaacaag gcaccaggta
      961 tataagagcc ttgttataac acctttaatc aaataaaata tttcggttgt agaaaatttt
     1021 cttacgtcag cggtgtttct ttcgctcagc gacttcccta ataattcgtg cattcctggc
     1081 aataatacac ttaaaacata ctgtccggag gttctgatga tgagatatac gccaaacaga
     1141 aaatatccga tcacacatat gaaaaggaat gatcagcagg tgaacagcga acgtataata
     1201 gacataagca tcggaactgc tgctggagtg cttgccttcg tattcctgaa cggcatgttc
     1261 tggggttata tgatcaggaa atttcgcagc tgaccgcagc catcgccgta aataactgag
     1321 ccggtacctg tggccgggtt tcctggcgga acatgtcaca agcaaaagag ttatttaatt
     1381 gcaaaaactg cccgatataa tatgcatata gtatcgggag gtcacagatg ccggaaacgg
     1441 ttctatattt tctcaggagc aaaaaaggca gcgaaagcct ggtcttctgg agtgtcgtac
     1501 tgcttgcagc ctgcgttccc gcaggcctgc tcctccgtac ggggataaca gtgtttgcga
     1561 ctccgctgct cagactcggt tcatatatcc tcatgctgcg ctacctttac atagtgctcc
     1621 taaaagactt tatcgcagaa tatgaagcaa taaacaggaa gttgtactcg cttggcaagt
     1681 ccatcgaaat caaagtaaaa aagaggatgc gtgaagtaga gagggtaaac agaaaacttc
     1741 tcgacaaatc aaaaacagat ccgatgacgg gcctgctgaa caaagctgcc ctgctcgacg
     1801 cggtcgaaag gctgatatcg aataaaccgg aaaaggaaca ctcgatcctg atgttcgata
     1861 tcgataattt taaagaaata aatgacaacc atggccacat tgccggagac aaatgcatca
     1921 aagcgctcgc agcagccaca ggaagcaact tcaggagctt cgacatcaca gggagatacg
     1981 gcggcgacga gttcatagcc gtattgccgg ataccggcgc caggcaggct ttcgcaatcg
     2041 ccgaacgttt caggaaagtg gtggataaaa cctcatctcc gcaccttacc atttccatag
     2101 gtatttcctc ataccctgca gacggcagtg acgccaggtc tcttatagaa gcggcggaca
     2161 aaggcctgta catctcaaaa gcaaaaggca ggaacgcggt aacttacagg gatgtctact
     2221 gacctgcggt gcagcttggt ttcctgcccc ggccccatac tgatcctgta ctccctttta
     2281 ttgcaaatat gaacatcagg acgccgcaga aggcaaacac tccgatgaaa gagcatttcc
     2341 tgattataaa cgatgcggcg ctcattcctg ccagttttcc gataccggcc atcatatcgc
     2401 tgaaagccgc gaccctggcc atatattccc tatcggcggc ggtttggcat cgtgccgcga
     2461 ggatgatgcc gctgaccgct atgatagttc cctcggcaaa ctgaagcagc aaaaccaccg
     2521 catatgcttt ggataccgca tatagcagcc atatgagcgc cgctgacgca aggcaggcat
     2581 aaaatatccg tcccccgcct ttgtccgttc tgccgtccat ctgctccgca aggaacatag
     2641 cgatcagatt ggctccatag tatgcagtaa tcatcatccc ccatcctttt gccgttactt
     2701 tcaggatatc gaaagcaaac ggataaaaag caagattgac ggatatgata cagaaaccga
     2761 tcactaaatc cgccgccagc agttcacgga tcgaagcgga agtcctgcag tacctgatgc
     2821 cgtctgtcaa agccgataac gcagactccc ggtgatactg cttcttgacg gcgtatctgc
     2881 caatctctcc ttctgtt
//
LOCUS       DAOSCC010000263         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_22498_length_2870_cov_4.131794, whole genome shotgun sequence.
ACCESSION   DAOSCC010000263 DAOSCC010000000
VERSION     DAOSCC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22498_length_2870_cov_4.131794"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLG72_10830"
     CDS             <1..597
                     /locus_tag="PLG72_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ81329.1"
                     /translation="HIVYVADYNRFEIMGQTRDDAAGEAFDKTARALGLGYPGGPLID
                     AQAKKGDPFAIDFPRVHFNDGSLDFSFSGLKTAVLNYLNSNAQKGIEISIPDVCASFQ
                     QAVVDVLVQNTIRAAELRGARKIALAGGVAANSQLRNCMKDAAEKIGADMFYPRPLLC
                     TDNAAMIGAAAFYEYKRGNISGLDLNAVASLKLGTACG"
     gene            complement(942..1682)
                     /locus_tag="PLG72_10835"
     CDS             complement(942..1682)
                     /locus_tag="PLG72_10835"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ81330.1"
                     /translation="MIPKISDIFQQKLDEIQSRVPVKIKGASSGTSFQQYLSDAVDNI
                     SGSAASASPDTVSDTLDASKASDIAIEDAGMSNDEAVSSSSANAIGLSESLRRALNAL
                     AAYRSGNYYNTAYKNELIEKCIIESSQKYHVDPNLIRAVIKQESNYNPYAVSKAGAQG
                     LMQLMPGTADALGVFNPFDIAENIDGGTRYLRDQLVAFDGDLTLALAAYNAGPGAVAR
                     YNGVPPYAETQNYVRKVLEYYLKYSNNE"
     gene            complement(1943..2290)
                     /locus_tag="PLG72_10840"
     CDS             complement(1943..2290)
                     /locus_tag="PLG72_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81331.1"
                     /translation="MKRSGNCSNCARGISMKVNPDIFCSIHGVVSRNYRCSKYVRKPA
                     QWSTEAPGTNYDAQWGNRCIDCEFFLVQHSESEHNPSVGCCKLFTVRNYDGSTRNACS
                     KFSRKPEKSVSAS"
     gene            2474..>2870
                     /locus_tag="PLG72_10845"
     CDS             2474..>2870
                     /locus_tag="PLG72_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOJ81332.1"
                     /translation="MKKPTVLRRRYIPYELVDISDDELLFRSDELLVTRWSSIRPRTD
                     MSGGISFTFLDRGYKVGRFYDSDRSFLFWYCDIIETEYDQATDTYTINDLLVDIKIYP
                     DGRVILLDADELAQALEVGLVSSAQAVKAL"
BASE COUNT          768 a          624 c          718 g          760 t
ORIGIN      
        1 catatcgtgt acgtggcgga ttacaacagg tttgaaataa tggggcagac acgtgacgac
       61 gcggccggcg aggcattcga caagaccgcg cgtgccctcg gcctgggata tccgggcggt
      121 ccgctgattg atgcacaggc taaaaaagga gatccttttg cgatagattt tccaagagtg
      181 cacttcaatg acggttcgct ggatttcagt ttcagcgggc tgaagactgc cgtactgaac
      241 tatttgaaca gcaacgccca aaagggcatt gaaataagta tccctgacgt ctgcgccagt
      301 ttccagcagg cggttgtgga tgttctcgtg cagaacacca tccgggcggc agagttaagg
      361 ggcgccagga agatagcatt ggccggcggt gttgcggcaa actcacagct caggaactgc
      421 atgaaggatg cggccgaaaa gatcggggcg gacatgttct acccgaggcc cctgctttgc
      481 accgataatg cggcaatgat aggagcggct gctttttatg aatataaaag aggaaacata
      541 tcaggcctcg atctcaatgc tgttgccagt ctgaaactgg gtaccgcctg tggataactt
      601 ttcattttcc acatttttca ttatgttttt tacatttaaa tcatcaaatg tgaattatgg
      661 aagaaaacga caaatggcaa aaaatgtcgg aaaccatagt accactcatg aagccggtat
      721 gtacttataa aagtacatta aacgctgtat tgcagcctgg aatagcaaaa ttttgatttg
      781 tagaaaagtg ctgaatatgg tgaaaacatg cctgtgaatt atgtggaaat tgctcagaaa
      841 gcgcgtaaat tgtaggaatc tcttgtgtgg ataacaatgt ggaaagtgtg gattacaggt
      901 gtcagaattt gataacatga cgggacaggt tcaataaaaa actactcatt atttgaatat
      961 ttcaaataat actcgagcac ttttctcacg taattctgag tttccgcata gggcgggacg
     1021 ccattgtacc tggcgacagc gcctggaccg gcgttatagg ctgcaagcgc cagcgtgaga
     1081 tcgccgtcga atgctacaag ctgatctctc aggtacctgg tgcctccgtc gatattttcg
     1141 gcgatatcaa aaggattaaa aactccaagc gcgtcagccg tccccggcat cagctgcatg
     1201 agcccctggg cgccggcttt tgaaacagcg tatggattat agttcgattc ctgttttatg
     1261 accgctctta tcaggtttgg atccacatgg tacttttgcg agctctcgat tatgcatttt
     1321 tcgatcagtt catttttgta agccgtattg tagtaattgc ctgacctgta ggctgccagt
     1381 gcgttcagcg ccctacggag agattccgac aggccaatgg cgtttgctga agatgaggaa
     1441 acagcctcat cattcgacat accggcatcc tcaatggcaa tatctgatgc cttcgatgcg
     1501 tccaatgtat cagagacagt atccggtgat gcggaggctg ccgatccgga aatattgtcc
     1561 acagcatccg acagatactg ctggaaggac gtgccggacg atgcgccctt tattttgaca
     1621 ggcaccctgc tttgtatctc atccagtttt tgctgaaaaa tatctgatat tttcggaatc
     1681 aagtgaatac ctccatcatg cttccatttt accacaaccg gcagcattga taaagcgatc
     1741 tcattttata tttcggacag gatgctgttt tacttgaacg gaaaaaagaa taccgatatg
     1801 tgtttcacgg ataaatccac gcgttctcat gggcaaatct gcaagcttac agtaaaagag
     1861 catgctttta aatcctgatg acaggattaa gtacgcggca tgaatgtctg cagtgtttat
     1921 cgtgacagcc atgttttgca catcaactgg ccgaaacgct tttttcaggc ttcctgctga
     1981 atttggagca ggcgtttctt gtgctgccgt cataatttct gactgtgaat agcttacagc
     2041 agccgacgga tggattgtgc tccgattcgg aatgctgaac caggaagaat tcgcaatcta
     2101 tgcacctgtt tccccactgg gcgtcatagt tggtcccggg agcctcggtg gaccattgag
     2161 cgggctttct tacgtacttc gagcacctgt agtttcttga aaccacccca tgtatgctgc
     2221 agaaaatatc aggattcacc ttcatgctga tacccctggc gcaattgctg caatttccgg
     2281 aacgtttcat aaactaccac cctgccctgt gatatacata tgtattaagc attttagcat
     2341 ataagcctgt gattggcaaa taatgggaaa taatcgtgcc gcttgattca gaagtgagtt
     2401 taaatgcatg tttcgtgatg taaacaactg gggtataatt atcctatatg tctgcagaaa
     2461 aaaggtgata accatgaaaa aaccgacagt actgcgaaga agatacatac cttacgaact
     2521 ggtcgatatt tcggatgatg agttgctctt caggagcgat gaattgcttg tcacaagatg
     2581 gtcgagcatc aggccgagga cggacatgtc cgggggcatt tcgtttacgt ttttggacag
     2641 aggctacaag gtgggcagat tttacgacag cgacaggagc tttttattct ggtactgcga
     2701 tataatagag acagaatacg accaggcgac cgatacatac actataaacg atttgctggt
     2761 ggatataaag atatatcctg acggaagggt aatactgctc gatgcggatg agctggcgca
     2821 ggcgctggaa gtaggcctgg tcagcagcgc acaggctgtg aaggcgctga
//
LOCUS       DAOSCC010000264         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_23738_length_2761_cov_2.884331, whole genome shotgun sequence.
ACCESSION   DAOSCC010000264 DAOSCC010000000
VERSION     DAOSCC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23738_length_2761_cov_2.884331"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLG72_10850"
     CDS             complement(<1..831)
                     /locus_tag="PLG72_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81333.1"
                     /translation="MNYVVHIFYILAITVKAFAYYGSVGIEKYPLITLMLTVFYLAAI
                     YFICGSKKLVYFCISIPVSLLMLTNVLYNRYFHMFFSLGTISQAGKLREVGGIALDLA
                     RPADLLLFADPVLPSIFAAFAKKRIRNKKQGTVWARFAPRAAVALVMAGSLVFLSVNP
                     KDSRLVTALSHQELFTWHVKDSFFRSEVINAGDVADMENAETEIVVPEQLDDIGYLHG
                     IASGRNLIVIQMEAFQDFLINAVYNGQELTPNLNRLVAGESIYFSSYYQQLGRGNTSD
                     A"
     gene            1000..2739
                     /locus_tag="PLG72_10855"
     CDS             1000..2739
                     /locus_tag="PLG72_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ81334.1"
                     /translation="MFCSKCGSKIPDGAKFCANCGQATQQAAGAASGPSGSMQGAGGA
                     DGVVAGGSTEKRGLGKGRLRKGFAVLAAAVAVILFAGFFIKANAWRLMSAKSYYGYLE
                     SRSGQISLNKLYRDSMKAAEVKPFSKDIVFTIDSIGTDAGIRGRLFEEFSLNLKIDHD
                     KNKTAGYVSVEFMDNELVDALFYRDRKTVGFGIPLLYDDYFRCDIDKLFTTISKLTGN
                     DMPEITVADKSPEQYRKEMDKDTRLLDERLAKYLKTLMNSIPGSNFSKKSEDDVTIYT
                     WAGGRRKKAAEFKNCRTVRFTVAEKDLYVAFDRVLADLEKDDEFIGLLAKYYDAGQLL
                     SIGRYRNYYGYESSLFGNDENDPESVIKNWITETRENLGDIFDPEDDYPLMEVKVIAD
                     SKDNIIFRQIITDYSFVSLATYSNPEGFRITELNAAGDEYDEERTAIYLYSGRKGKGA
                     MWHTDFGTGEISFMSSGEGKSDIGMEYGMYKLKMSQMFSSYELDITAEKDQNEKNTDL
                     LRLRLYVDNERIGSVKAVVRDLKDKSKLTFNKNKAKDISELDQYDLMDIGESIMENFE
                     AGVAGRMGSFLGW"
BASE COUNT          799 a          585 c          718 g          659 t
ORIGIN      
        1 cgcgtctgat gtattgcctc tgccgagctg ctggtaataa ctgctgaaat atatggattc
       61 gcctgcaacc agcctgttta ggttaggcgt gagttcctgt ccgttgtata ccgcgttgat
      121 aaggaagtcc tggaacgctt ccatctggat cacgatgagg ttccttcccg atgcgatacc
      181 gtgcaggtaa ccgatgtcat ccaactgttc cggaacgacg atctctgttt ccgcgttctc
      241 catgtcggcc acatcaccgg cgttgattac ttcagacctg aaaaagctgt ccttcacatg
      301 ccatgtgaac agttcctggt ggctcaacgc tgtgaccagc ctgctgtcct tcggattcac
      361 cgaaaggaaa accagcgatc cggccatcac cagggctact gcagcccttg gagcaaaacg
      421 tgcccatact gtaccctgct ttttgttacg tatccgtttt tttgcaaatg cagcgaatat
      481 ggagggaagg acaggatctg caaacagcag caaatctgcc ggccttgcca ggtcaagggc
      541 aataccgccg acttccctga gcttgcctgc ctgcgatatg gtgcccaggg agaagaacat
      601 gtggaaatac ctgttgtaca gaacgtttgt cagcatgagc aaggagacag gtatgcttat
      661 gcaaaaataa accagttttt ttgagccgca tatgaagtat atggcggcca ggtaaaagac
      721 tgtcagcatc aatgtgatga gcggatattt ttcgatgccg accgatccgt aataagcaaa
      781 tgcctttacg gttattgcca ggatgtaaaa tatgtgtact acgtaattca tgtaatcacc
      841 gtcttatata ttacaacatt ttgacggacc ggcacagttt ttttctgccg ggacacaggg
      901 atttttttcg catgcgaacg acattttttt tgcctttccc gtacaatatg gtataatatg
      961 tcattatgtt attatatcaa acacatgggg ggtctactga tgttttgttc aaaatgcggc
     1021 agtaaaattc ctgatggagc gaaattctgt gccaactgcg gacaggcgac gcagcaggcg
     1081 gccggagcgg catccggacc atcaggcagt atgcagggag caggtggagc agacggagtc
     1141 gtggcaggag ggagcacaga aaaaagaggc ctgggcaaag gcagactgcg caaaggcttt
     1201 gctgtactgg cagccgctgt cgccgtcata ctttttgcgg gcttttttat aaaggcaaat
     1261 gcatggagac tcatgtccgc aaaatcatat tatggctacc tggaatccag aagcgggcag
     1321 atttctttga ataaacttta cagggattcg atgaaggccg ccgaggtaaa accattcagc
     1381 aaagacatcg tatttacgat cgacagtatc ggcacggatg cggggatacg cggaaggctt
     1441 tttgaagaat tcagcctgaa tctcaagata gatcatgata aaaataaaac cgcaggctat
     1501 gtttccgttg aatttatgga taacgaactg gttgacgcct tgttttaccg ggaccggaaa
     1561 acggtgggtt ttgggatacc tctcctgtat gatgattatt tccggtgcga catagacaaa
     1621 ctgtttacaa ccatcagcaa actgacaggc aacgatatgc cggaaattac agtggctgat
     1681 aaatcgccgg aacagtacag gaaagaaatg gacaaggata ccaggctgct tgacgagaga
     1741 ctggcgaaat atttgaaaac tctgatgaac agtatccctg gatcaaattt cagcaagaag
     1801 agtgaagacg atgtaaccat atatacatgg gcgggcggca gaaggaaaaa ggctgccgaa
     1861 ttcaaaaact gcaggacagt cagattcact gttgccgaaa aagaccttta tgtggcattt
     1921 gacagggtac tggccgatct ggagaaagat gacgagttta taggcttgct ggcaaaatat
     1981 tatgatgccg gccagttact gtcgattggc agatatcgta actattacgg atacgaaagc
     2041 agtctgtttg gcaatgatga aaacgatcct gagagcgtga taaaaaactg gatcacagag
     2101 acaagggaaa acctcggtga cattttcgat cccgaagacg attatccgct gatggaggtt
     2161 aaagttatcg cagacagcaa agacaacatc attttcaggc agatcatcac ggattattca
     2221 tttgtttcgc ttgctactta ttcaaaccct gaaggattca ggataacaga actgaatgct
     2281 gccggtgacg aatacgacga agaacgcacc gcgatatacc tgtacagcgg caggaaaggc
     2341 aagggcgcca tgtggcatac agacttcgga accggtgaga tttcctttat gagcagcggc
     2401 gaagggaaaa gtgacatcgg catggaatac ggcatgtaca aactgaagat gagccagatg
     2461 ttcagcagtt atgaactgga catcacggca gagaaggatc agaatgaaaa gaatacagac
     2521 ttgctcagac tgcgtcttta tgtggacaac gaaaggatcg gcagtgtgaa agccgttgtc
     2581 agggacctga aggacaaatc taagctgaca ttcaataaaa acaaggccaa agacatctcc
     2641 gaactggacc agtatgacct gatggatatc ggagagagca tcatggaaaa cttcgaggcc
     2701 ggcgtcgcgg gcaggatggg aagtttcctg ggttggtgag attatgattc aaaaaaagta
     2761 t
//
LOCUS       DAOSCC010000265         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_23799_length_2756_cov_4.684561, whole genome shotgun sequence.
ACCESSION   DAOSCC010000265 DAOSCC010000000
VERSION     DAOSCC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23799_length_2756_cov_4.684561"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLG72_10860"
     CDS             complement(<1..925)
                     /locus_tag="PLG72_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81335.1"
                     /translation="MKAFKLIYLLTGILLAAGVLLTSMRGLPGEDLELYEKACMLEDG
                     RLDRIWPGLYISRYPVAIRKGNAEYVIFNGDIKKRKPVLPVIACTAYPSEDGVYILVP
                     SKSVMDSVGQIAEGFSGSFDDFLIDQFSIDAQKMSDSQYISVLCHEAMHALQLSCWER
                     KIIVLAEDIADAGIEARIAETESDPEIRAMYEKQADLLYRLAVSDDGVPVAETVREYI
                     RTRKDTQALLSAKTGIDIDAVRSYADLYELLEGTARYAEAKAALALSDEELYGQYLHS
                     LKETSYGRENYYKSGMGICMLLDRFDPDWKQD"
     gene            complement(927..1685)
                     /locus_tag="PLG72_10865"
     CDS             complement(927..1685)
                     /locus_tag="PLG72_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81336.1"
                     /translation="MLALIHNTFRERIRGKTFYVVAFIGMAVMLLIATGDSNLTINGK
                     KITGFEQMVPVALSITGFVSSLLAVMVSLRTIPDEFERKATHLVLIRDIKPWQYMFSL
                     TVGNMLASLFCMLSLYVSLFVFCAAYGKLGLILPTFLCIMVMSINSMFLSAAVSVVSI
                     KAPVYVAGFAGILIYAAGILHGVLDALANTTQGASAILARTMLFLIPDFSAVQQQASN
                     ILTGAFVDPEPIFGSLMLLYLVLSLTFVLFRREV"
     gene            complement(1666..2370)
                     /locus_tag="PLG72_10870"
     CDS             complement(1666..2370)
                     /locus_tag="PLG72_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ81337.1"
                     /translation="MKILECCGLVKTYGKVKAVDGLSFSVERGEVLGLLGPNGAGKTT
                     TIKMILGLTGLTGGKVLIDKNTKIGYSPETPYFHPFLTGCEVMRFYSRLQKLDRQTAE
                     QQIGRLLELAGLSGAAEKKVKNYSKGMLQRLAFAQALLGDPELLILDEPTSGLDAVGR
                     IEMLELIELLKREGKTIIINSHILSDIEKVADRTIIIANGRLAGMTDKNSLAGSSLEE
                     LFIRAVGGAVHACADT"
     gene            complement(2515..>2756)
                     /locus_tag="PLG72_10875"
     CDS             complement(2515..>2756)
                     /locus_tag="PLG72_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81338.1"
                     /translation="SATTLMKLIRAGNWREFAKGRLSVSVYCRDGRNIVLNSTIRTPE
                     GAYVYITKNPEICLPVNAEDNELGDAILGLIKKCR"
BASE COUNT          663 a          761 c          624 g          708 t
ORIGIN      
        1 gatcctgttt ccagtccggg tcgaacctgt ccagaagcat gcaaataccc ataccgcttt
       61 tgtaatagtt ctcccttcca taggatgttt ccttcaaaga gtgcaaatat tgtccgtaca
      121 gttcttcatc tgaaagcgcg agcgcagcct ttgcctcggc ataccttgcc gttccctcca
      181 gcagttcata caggtcggcg taacttctca cggcgtcgat atcgattcct gtctttgcgc
      241 tcagcagtgc ctgcgtatct tttcttgtcc tgatatactc cctgacagtt tcggcaacgg
      301 gcacgccgtc gtccgaaacc gccagccggt acagcaggtc tgcctgtttt tcgtacatgg
      361 cacggatttc cggatcggat tcagtttctg cgatcctcgc ctcgataccg gcatctgcga
      421 tatcttcggc caaaactatt attttcctct cccagcagct tagctggagg gcatgcatcg
      481 cctcatggca gagtaccgat atatattggc tgtccgacat cttttgggcg tcgatcgaaa
      541 actgatcgat aagaaagtcg tcaaagctgc cggaaaaacc ctccgcaatc tgtccgaccg
      601 aatccatcac cgatttggac ggcaccagga tatatacccc gtcttcggaa ggatatgccg
      661 tacatgcaat gaccggcagg accggtttcc ttttcttaat gtctccgttg aaaatcacgt
      721 actctgcatt gcctttccgt attgcgacag gataacggct gatatacagg cccggccata
      781 tccggtcaag tctcccgtcc tccagcatgc aggctttctc atacagttcc aggtcctccc
      841 caggaagccc ccgcatggaa gtcaacagca caccggctgc cagaagtata ccggtaagca
      901 gataaatcag cttaaatgct ttcatgtcat acctccctcc tgaaaaggac aaacgtcagt
      961 gacaagacca ggtataacag catcagcgag ccaaatatcg gttccggatc cacgaaagcg
     1021 cctgtcagta tgttcgaagc ctgctgctga accgctgaaa aatccggtat caaaaacagc
     1081 attgtacggg caaggatggc actggcacct tgagttgtgt ttgccagggc gtccaggacg
     1141 ccatgcagta taccggccgc ataaatgaga atgcccgcga agcctgcaac ataaacaggc
     1201 gctttgatgc tcacgacact tacggccgca ctcaggaaca tcgaattgat gctcataacc
     1261 atgatgcaca ggaatgtcgg cagtataagc ccgagcttgc cgtaagcggc acagaagaca
     1321 aacagcgaga cataaagaga aagcatgcag aaaaggctcg cgagcatgtt tccgacagtg
     1381 agcgaaaaca tgtactgcca tggctttatg tcccttatca gcacaagatg agtcgctttc
     1441 ctttcaaact cgtccgggat cgtccgaagt gataccatta cagcaagcag gcttgacaca
     1501 aaacctgtta tcgaaagggc tacaggcacc atctgttcga atcccgttat ctttttcccg
     1561 tttatcgtaa gattgctgtc cccggtggca ataagcagca tgaccgccat cccgataaat
     1621 gcgacgacat agaacgtctt cccgcgtatc cgttccctga atgtgttatg tatcagcgca
     1681 agcatgcacg gcacctccca cggctcttat aaacagttcc tcaaggctgc tgccggccag
     1741 gctattcttg tcagtcatgc cggccagcct tccgtttgcg atgattatcg tcctgtctgc
     1801 gactttttcg atatcactta agatatggga attgataatg atcgttttgc cttcacgttt
     1861 cagcagttca atcagttcaa gcatttctat cctgccgacg gcatcaaggc ctgatgtggg
     1921 ttcgtcaagt atgagcagtt caggatctcc cagcagagcc tgtgcaaacg caagtctctg
     1981 cagcataccc tttgagtaat ttttgacctt tttttcagct gctcctgaca atccggcaag
     2041 ctccaggagt cttccgatct gttgttcggc agtttgcctg tccagtttct gcagtctcga
     2101 atagaaccgc attacctcgc agcctgtcag aaacggatgg aagtaaggtg tctcagggga
     2161 ataaccgatc ttcgtgtttt tatcgataag caccttcccg ccggtcagcc cggtaagccc
     2221 aagtatcatc tttattgtgg tcgtcttgcc ggctccgttc ggcccgagga gtcccagtac
     2281 ctctcccctt tccacgctga aggaaaggcc gtcgaccgcc ttaactttgc cgtatgtctt
     2341 gaccagtccg cagcattcca gtattttcaa agctttcacc tccacattgt gataataaag
     2401 gcaaaagctt taaatacact tcacacagac ttaaaattac cttaagaaaa tgcgggtgaa
     2461 agagaatgga tagtcccgga tatagatagt cccggatatt gcagtaatca aagtctatct
     2521 gcactttttg atgagtccga ggatcgcatc cccaagttcg ttgtcttctg cgttcacagg
     2581 cagacatatc tccgggtttt tcgtgatata gacatatgcc ccttcaggag tccttatggt
     2641 ggagttcagc actatgttcc tgccgtccct gcagtatacc gatacgctga gcctgccttt
     2701 cgcaaactct ctccagtttc ccgcacggat cagcttcatc aaagtcgttg cggagg
//
LOCUS       DAOSCC010000266         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_23849_length_2751_cov_4.540430, whole genome shotgun sequence.
ACCESSION   DAOSCC010000266 DAOSCC010000000
VERSION     DAOSCC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23849_length_2751_cov_4.540430"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..880
                     /locus_tag="PLG72_10880"
     CDS             29..880
                     /locus_tag="PLG72_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOJ81339.1"
                     /translation="MSTPLVLDGKALAKSIEQELTVRVQRIKDKTGITPILATIIVGN
                     DPASVTYVRMKGNACRRIGMESLKVELPEETTTQQLIDRIRELNHDGNVHGILLQHPV
                     PAHIDEMACFLSIDVSKDVDGVNPNTFGRMALQMDSFKCATPYGIITLLKHYNIRIEG
                     KEAVVIGRSPILGKPVAMMLLNENATVTICHSKTKDLPDVVRRADIVVAAVGKPRFVK
                     ADWVKEGCVLVDAGYNPGNIGDIDLENAISKCSAYTPVPGGVGPMTINRLICQTVESA
                     EKKLGLA"
     gene            1124..>2751
                     /gene="lepA"
                     /locus_tag="PLG72_10885"
     CDS             1124..>2751
                     /gene="lepA"
                     /locus_tag="PLG72_10885"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ81340.1"
                     /translation="MSHNRQNNIRNFCIIAHIDHGKSTLADRLIQMTGVLTEREMQEQ
                     VLDTMDIERERGITIKAQAVRMVYKAKDGQEYILNLIDTPGHVDFNYEVSRSLAACEG
                     AVLVVDASQGIEAQTLANVYLALEHDLEIIPVINKIDLPSARPEEVKKEIEEVIGLDC
                     SEAPEISAKNGINIEAVLEQIIKHVPAPKGSEDDPLRALIFDSLYDSYKGVIVHVRIM
                     EGAVRTGDTIRLMSTGKEFIVTEVGYFRPGQYAPCDMLLAGDVGYIAASIKNVRDTRI
                     GDTVTLADRPASEPLPGYKKVQSMVFCGIYPADGAKYNDLRDALEKLHLNDAALTFEP
                     ETSGALGFGFRCGFLGLLHMEVIQDRLEREYDLDLVTTAPSVVYRITLTDGKVMYIDN
                     PTNMPPAASIEKMEEPVVKATIMTPEEYVGNIMELSQERRGTYKDMSYIDRKRVMLTY
                     ELPLNEIIYDFFDALKSRTKGYASFDYELIGYQESELVKLDIMLNGEIVDALSFIVHK
                     DKAYARGRRIAEKLKDVIPRHQFEIPIQACIGGK"
BASE COUNT          750 a          654 c          803 g          544 t
ORIGIN      
        1 ttattacata agaaagaagg tagataacat gagcacacca ttggtactgg acggcaaagc
       61 gctggcgaaa agcatcgagc aggaactgac ggtacgggta cagagaataa aggataaaac
      121 cggcattact ccgatcctgg ctacgataat agtggggaac gatccggctt cagtgacata
      181 cgtgagaatg aagggcaacg cctgcagaag gatcgggatg gaatcgctca aagtcgaact
      241 gccggaggag acgaccacac agcagcttat cgacaggatc cgggaactga accacgacgg
      301 gaatgtacac gggatattgc tgcagcaccc agtaccggcc catatcgacg agatggcatg
      361 cttcctcagc atcgacgttt caaaggacgt cgacggcgtt aacccgaaca cattcggaag
      421 aatggcgctg caaatggatt cgttcaaatg cgctacaccg tatgggatca taacgctgct
      481 gaagcattac aatatccgga tagaaggcaa ggaagccgta gtgataggca ggagcccgat
      541 cctgggcaag ccggtggcta tgatgctcct taatgagaat gcgacggtca ccatatgcca
      601 ctccaaaacg aaggacctgc ccgacgtagt gaggcgcgcc gacatcgttg tggcggcggt
      661 cggcaagccc aggttcgtga aagccgactg ggtaaaggaa ggctgcgtgc tggtggatgc
      721 agggtataac cccggcaata taggggatat agaccttgag aatgccataa gcaagtgctc
      781 ggcatacacg ccggtgcccg gcggagtcgg gccgatgacc atcaaccgcc tgatctgcca
      841 gacggtggaa tcggcggaga agaagctcgg acttgcataa atccgtatct gcacgggatc
      901 atcgccacgg gtttattgca ttatcaatag accaggcttt ccgaaaactg atacctttat
      961 tttgtcgatt tccgatgtta taattgtttt gcgtttatcg ggaacgcgtt tttttgaaat
     1021 tggccggacc gcatttgctt agcgtacagc aggccggatt tgcaccggat cattttcttt
     1081 cggccattat tactttttca tgattcacga aggggaagga tgaatgtcgc acaacaggca
     1141 aaataatata aggaattttt gcataatcgc gcatatagac cacggcaaat cgacgctggc
     1201 agaccgcctc atccagatga caggcgtgct gacggagagg gagatgcagg agcaggtcct
     1261 cgacaccatg gacattgaga gggaacgcgg cataactatc aaggcgcagg ccgtcaggat
     1321 ggtatacaag gcaaaggacg ggcaggagta tatactgaac ctgatcgata cccccggcca
     1381 tgtcgatttc aactacgagg tatccaggag ccttgcggca tgcgaaggcg cagtacttgt
     1441 cgtggatgcg tcgcagggca tcgaggcgca gacgctggcg aacgtttatc ttgcgcttga
     1501 acatgatctt gagataatac cggtcatcaa taaaatagac ctgccaagcg cgcggccgga
     1561 agaggtcaag aaggagattg aagaagtgat cgggcttgac tgttccgaag cgccggaaat
     1621 ctctgcgaag aacggcataa acatcgaagc ggtgctggaa cagatcataa agcacgttcc
     1681 tgcgccgaag ggcagtgagg acgatccttt gcgggcgctt atattcgact cgctatatga
     1741 cagctacaaa ggcgtaatcg tgcatgtccg gatcatggaa ggagcagtca ggacgggcga
     1801 taccatccgg ctgatgtcta caggcaagga atttatagtg accgaagtcg ggtatttcag
     1861 accggggcaa tatgcaccgt gcgacatgct cctggcaggc gatgtaggct atatcgccgc
     1921 aagcatcaaa aatgtaaggg ataccaggat aggcgacacc gtcacactgg cggacaggcc
     1981 ggcaagcgag ccgctgccgg gttacaagaa ggtccagtct atggtgttct gcggcattta
     2041 tccagctgac ggcgcaaaat acaacgatct gagggatgcg ctggaaaaac tgcatctcaa
     2101 tgacgccgcg ctcacattcg agccggagac gtcgggagct ctgggattcg gcttcaggtg
     2161 cgggttcctc ggcctgctgc atatggaagt catccaggat cggctggaga gggagtacga
     2221 cctggacctg gtgaccactg ctccgagcgt tgtttacagg atcacgctca cggacggaaa
     2281 agtgatgtat atagacaacc cgacgaacat gccgcccgcc gcatccatag aaaagatgga
     2341 ggagcctgtc gttaaggcta ccatcatgac tccggaagaa tacgtcggga acataatgga
     2401 gctttcgcag gaaagaaggg gcacttataa agatatgtcg tacatcgacc ggaagagggt
     2461 catgctgacg tacgaactgc ccctcaacga gataatttac gactttttcg acgcgctgaa
     2521 gtccaggacc aagggctatg cgtcgttcga ctacgaactg atcgggtacc aggaatcgga
     2581 acttgtgaag ctggacataa tgctcaacgg cgagatcgtg gacgctctgt cgttcatcgt
     2641 ccacaaggac aaggcttacg caagggggcg caggatcgcc gagaaactga aagacgtcat
     2701 accgaggcat cagttcgaga taccgataca ggcatgcata ggcggcaaaa t
//
LOCUS       DAOSCC010000267         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_23980_length_2738_cov_4.317555, whole genome shotgun sequence.
ACCESSION   DAOSCC010000267 DAOSCC010000000
VERSION     DAOSCC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23980_length_2738_cov_4.317555"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="mutL"
                     /locus_tag="PLG72_10890"
     CDS             complement(<1..483)
                     /gene="mutL"
                     /locus_tag="PLG72_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ81341.1"
                     /translation="MGKIIILDENTVNKIAAGEVVERPASVIKELVENSIDAGATTIN
                     IEIKNGGISYIKVADNGIGIDEDDMELAFERHATSKIMYPDDLESVSTMGFRGEALAS
                     IAAVSNIELTTRTKGSIYGTYIKLSGGTILEKNQKGCPVGTTIIVKDLFFNTPARYKF
                     L"
     gene            complement(486..>2738)
                     /gene="mutS"
                     /locus_tag="PLG72_10895"
     CDS             complement(486..>2738)
                     /gene="mutS"
                     /locus_tag="PLG72_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ81342.1"
                     /translation="KNNYLVSIYAKKHMYGFAAVDVSTGYFATTRITWGNTLGKLLDE
                     IAKYAPAEIITNRAFFDSKEAAGQLTGRFSAYISQLDDACYEDGFCIDLLRSHFGSTI
                     LPDEEAELCVNASGALLYYLEQTQKSSLGHIQQITPYKLEEYMILDMTARRNLELTET
                     MRERSRKGSLLWVLDRTMTSMGARALRRWVEQPLINIGDINERLEAVEELKDKFMVRM
                     ELRELFRKVYDIERLISRIVLGNANCRDMIALKNSLGQIPYIKKMLDNCGSALISKNN
                     AHMDELSDIYELIESSIVDDPPISVKEGGIIKSGFDEEVDRLRDAATNGKEWILSLEA
                     REREKTGIKNLRIGYTKVFGYYIEVTKSNLHMVPQDYIRKQTLVNCERYVTQELKEME
                     ETVLGAESRVVDLEYQIFVEIRQRVATQTTRIKQTAQCIAELDAICSLAEVADRENHC
                     KPVMTNDGMIKIENGRHPVVEKTLETGSFVPNDTLLDMDENRLAVITGPNMAGKSTYM
                     RQVALIVLMAQIGSFVPADSAVIGIADRIFTRVGASDDLAAGQSTFMVEMSEVAYILE
                     NATPKSLLVLDEIGRGTSTFDGLSIAWSVIEYISDSEKVGSRTLFATHYHELTELEGK
                     LPGIKNYCIAVREEGDDIIFLRKIIRGGADGSYGIQVAKLAGLPQSVTERAKELLKEL
                     EAADISKKKAHNRRKVLEGQVEFFIPENPEGKIADEIVEELRAVDISTLTPLDALNVL
                     YKLQIKARKG"
BASE COUNT          570 a          744 c          576 g          848 t
ORIGIN      
        1 cagaaactta taccttgcgg gagtattgaa aaacaagtct tttacaatta tggttgttcc
       61 tacaggacat cccttttgat tcttttctaa tatagtacca ccgcttaact taatataagt
      121 accataaata ctgcccttag ttcttgttgt aagctcaatg tttgaaactg ctgcaatgct
      181 tgccagggct tcccctctaa aacccatagt tgatactgac tcaagatcat ccggatacat
      241 tattttactg gttgcatgcc tttcaaaagc taattccata tcatcttcat caatgcctat
      301 gccattgtct gcaactttta tatatgatat tcccccattt ttaatttcaa tgtttattgt
      361 tgtcgcacct gcatctatgg aattttccac caattccttt atcacagagg caggcctttc
      421 gacgacctca ccggcagcga tcttgttcac ggtattctca tccagaatga ttatctttcc
      481 catattcaac ccttcctggc tttaatctgg agcttataaa gaacattcag ggcatccagc
      541 ggcgtcaggg tcgatatgtc cacggccctg agttcttcca cgatctcgtc ggcgatcttc
      601 ccttcagggt tttcaggtat gaaaaattcc acctgtcctt caagcacttt tctcctgttg
      661 tgcgccttct tcttgctaat gtcggctgcc tcaagctctt tcaggagttc tttcgccctt
      721 tccgtcaccg actggggaag cccggccagc tttgctacct ggatgccgta gcttccgtcg
      781 gctcctccgc ggatgatctt ccttaagaat atgatatcgt ctccctcttc cctgacagcg
      841 atgcagtaat tctttatgcc gggaagtttg ccttcaagtt cggtgagctc atgatagtgg
      901 gtggcaaaca aagtcctgct ccccactttt tcgctgtccg atatatattc tatcaccgac
      961 catgctatgc tcaggccgtc aaacgtgctg gtgccccttc ctatctcgtc cagcaccagc
     1021 aggctttttg gcgtggcatt ttccaatata tatgccactt ccgacatttc gaccatgaaa
     1081 gtgctctggc cggctgccag atcatcggaa gctccgaccc tggtgaaaat cctgtccgca
     1141 atgccgatga ccgccgagtc agcaggtaca aaactcccga tctgcgccat cagtacgatg
     1201 agcgccacct gccgcatata tgtcgatttg cctgccatgt tgggacccgt gattactgcc
     1261 agcctgtttt catccatgtc gagcagtgta tcgttcggta cgaaggatcc ggtttcgagc
     1321 gtcttctcga ccacagggtg tcttccgttt tctattttta tcatgccgtc attggtcatt
     1381 accggcttac agtggttttc ccggtcggcg acctcggcaa gagagcatat agcgtcgagt
     1441 tcggcaatgc actgggcggt ctgttttatc ctggtggtct gcgttgccac tctctggcgt
     1501 atttcgacaa atatctggta ttccaggtcc actaccctgc tctcggctcc gaggaccgtc
     1561 tcttccattt ccttgagttc ctgtgtcaca tacctttcac agttgaccag tgtctgtttc
     1621 cttatgtaat cctgcggtac catgtgtaaa tttgatttgg tgacttctat gtaatagccg
     1681 aatacctttg tgtacccgat cctgaggttc ttgattcctg tcttctcacg ttccctggct
     1741 tccagggaca gtatccattc ctttccgttg gtagctgcgt ccctcagcct gtcgacctct
     1801 tcatcgaatc cggacttgat gatcccgcct tctttcactg aaatcggagg atcatctact
     1861 atggaactct caatcaattc atatatatcg gacaattcgt ccatatgggc gttgtttttc
     1921 gatatcagcg ccgatccaca gttatcgagc atcttcttta tatacgggat ctgtccaagg
     1981 gaatttttca gtgctatcat atccctgcag ttggcattgc ctaggacgat cctgctgata
     2041 agtctctcga tgtcatagac cttcctgaac agttcccgca gttccatcct gaccatgaat
     2101 ttgtccttca gttcctcgac cgcctcaagc cgttcattga tgtcgcctat gtttatcagt
     2161 ggctgttcca cccatcttct gagtgccctt gcacccatgg atgtcatcgt cctgtccagc
     2221 acccacagca atgaaccttt gcgggacctt tcacgcatcg tctccgtcag ttccaggttt
     2281 ctgcgggctg tcatgtcgag tatcatatat tcttcgagct tgtagggagt gatctgctgt
     2341 atatggccaa ggctcgattt ctgcgtctgt tccaggtaat acagcagggc gcccgatgca
     2401 ttgacgcaga gttccgcctc ttcatccgga aggatcgttg atccgaaatg acttcggagc
     2461 aggtcaatgc aaaatccatc ttcgtaacag gcatcgtcca gttgggaaat atatgcgctg
     2521 aacctgcctg tcagctgccc ggcggcttcc tttgaatcaa aaaaagcgcg gttcgtaatg
     2581 atctcggcag gagcatattt tgctatttcg tccagaagtt tgcccagcgt attcccccaa
     2641 gtgatcctcg ttgtggcaaa atatcctgtg gatacgtcaa ctgccgcaaa gccgtacatg
     2701 tgctttttag catatatcga tacgagatag ttgttctt
//
LOCUS       DAOSCC010000268         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24075_length_2731_cov_4.924888, whole genome shotgun sequence.
ACCESSION   DAOSCC010000268 DAOSCC010000000
VERSION     DAOSCC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24075_length_2731_cov_4.924888"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLG72_10900"
     CDS             <1..897
                     /locus_tag="PLG72_10900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ81343.1"
                     /translation="ERWGRFTLRRPDPQAIWPAAGNEKAWKNVDAHYHRSSSGGGRWE
                     YIRKLPPQWTIRYGDLQFYVEPTGFKHTGLFPEQAVNWKWIENKIKNAMGADKAGCGT
                     WDGSGHAYGNGRKLPGSDAGGTGNRISVLNLFAYTGGATVAAAHAGAEVCHVDASKGM
                     VTRAKENLALSGLADRPVRFIVDDVVKFVAREIRRGRKYDAVIMDPPSYGRGPNGELW
                     KIEDNIYGLITMCMDVLKEKPLFFIVNSYTTGLSPTVIGNMLKMSVQRKFGGSVTADE
                     IGLPVSASGLVLPCGATGRWEA"
     gene            901..1626
                     /locus_tag="PLG72_10905"
     CDS             901..1626
                     /locus_tag="PLG72_10905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ81344.1"
                     /translation="MDMGSSRDIKIIYEDNHLLVVEKPQNILTQGDQTGDEDLLTILK
                     EDIRQRYNKPGNVYLGLVHRLDRPAGGAMVFAKTSKAASRLSDQVRTGTFGKKYLAVV
                     HGVPDEPTGTLVNYLVKDEAANTVKAVDGIGDPASDLPAGAKKAVLDYEVIEAADGLS
                     LVRIKLHTGRPHQIRVQFAAIGHPLYGDQKYGVSVNAPGMQLALWSVEISFIHPTLRE
                     PVSFSSDPPNAYPWTLFRQPLLL"
     gene            complement(1637..2482)
                     /locus_tag="PLG72_10910"
     CDS             complement(1637..2482)
                     /locus_tag="PLG72_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ81345.1"
                     /translation="MSDNRNNNIMNSNTRILLTRFALPVIGVIIAARINKWLGIAALL
                     VYFLILVYISRARIYTIIGTRKYGFGQLDEAIKWFGRAYATNRASVRAAVNYAYLLLK
                     NAELQKSEEILQKVMKEHPENQEIPYIKSIMALVLWKKGELDQAVEMLGEVIQTYKTT
                     SVYGSLGFMLIQKGDLEKALQFNLEALDFNPRDNIINDNLGQNYFLLGQYDKAREVYE
                     KLLAKAPSFPEPYYNYGLLMEKLGNKEEALESMKKALNMKFSYLSTIAREEVETAISR
                     LENAG"
BASE COUNT          653 a          665 c          760 g          653 t
ORIGIN      
        1 gagcgatggg gcagatttac attaaggagg cccgatcccc aggcaatatg gcctgccgcc
       61 ggaaacgaga aggcatggaa gaatgttgat gcccactatc acaggagcag cagcggagga
      121 ggcaggtggg aatacataag gaagcttccg ccgcaatgga cgatacggta cggcgacctg
      181 cagttctatg tggagcctac cggcttcaag cacaccggcc ttttcccgga acaggccgtg
      241 aattggaagt ggatagagaa caagataaaa aacgccatgg gggcggataa agccggatgc
      301 ggaacatggg acgggtcagg gcacgcatat ggaaacggaa ggaaacttcc cggaagcgat
      361 gccggaggaa cggggaatag gataagtgta ctcaacctgt tcgcatacac ggggggagcc
      421 acggtcgcgg cagcgcatgc cggcgccgag gtatgccatg tggacgcatc gaagggaatg
      481 gtcaccaggg cgaaggagaa ccttgccctg tccggccttg cggacaggcc cgtcaggttc
      541 atagtcgacg atgttgtgaa atttgttgca cgtgaaatca ggaggggcag gaagtatgac
      601 gccgtgatca tggatccgcc ttcatatgga agagggccca acggtgaatt gtggaagatc
      661 gaagataata tatatggcct gataaccatg tgtatggatg tgctgaagga aaagccgctg
      721 tttttcattg taaattcata tacgaccggg ctttcgccga cggttatcgg gaacatgctg
      781 aagatgtcgg tacagcgcaa gtttggcggc agcgttaccg ccgatgagat aggtctgcct
      841 gtcagcgctt caggccttgt gctcccgtgc ggcgcaactg gcagatggga agcgtgagtg
      901 atggatatgg gcagcagcag agacatcaaa atcatatatg aagacaacca cctgcttgtg
      961 gtcgaaaagc ctcagaacat actgacacag ggcgatcaga caggcgatga ggacttgctg
     1021 acgatcctga aagaagatat caggcaaagg tacaacaagc cgggcaacgt atatcttggc
     1081 ctggtgcaca ggctcgaccg ccctgccgga ggggcgatgg tctttgccaa aacgtccaaa
     1141 gcggcttcac ggctgtcgga ccaggtaagg accggaacat tcggcaagaa atatcttgca
     1201 gtcgtgcacg gagtgccgga tgagccgaca ggtacgctgg tcaattacct tgtaaaggat
     1261 gaagctgcga acactgtaaa agcagtggac ggcatcggcg atccggcgtc agacctgcct
     1321 gccggcgcaa aaaaagcggt tttggactat gaagtgatag aagccgcaga cgggctgtcg
     1381 ctggtgcgga taaaactcca taccgggcgg cctcaccaga tacgtgtgca gttcgccgcc
     1441 atcgggcatc cgctgtatgg ggaccagaag tacggcgtat cagtgaatgc gccggggatg
     1501 cagcttgccc tctggtccgt cgagatcagc tttatccatc ccacgctcag ggaaccggtc
     1561 tctttcagtt cggatccgcc gaatgcttat ccctggacct tgttcagaca gcctctcctg
     1621 ttgtgaatgt ggaaattcag cctgcgtttt caagccggct tatggctgtt tcgacttctt
     1681 cccttgctat agtgctcaga tatgagaatt tcatattgag ggcttttttc atcgattcca
     1741 gcgcttcttc cttattccct agcttttcca tcaaaagccc gtaattatag tatggttccg
     1801 ggaatgaagg ggcttttgcc agcagctttt catatacttc cctggccttg tcatactggc
     1861 ccagcaggaa atagttctgg ccaagattgt catttatgat gttgtcccgc gggttgaaat
     1921 cgagggcttc aagattgaac tgcagcgcct tttcgaggtc gcctttctgt atcagcataa
     1981 agcccaggct tccgtagaca gacgtggtct tgtatgtctg aatgacttcc cctagcattt
     2041 ccacggcctg gtcaagttcg ccctttttcc acagcacgag agccatgatg gattttatat
     2101 aagggatctc ctggttttcc gggtgttcct tcatcacttt ctgaaggatc tcctcggatt
     2161 tctgcagttc cgcgttttta agaagcaggt aagcataatt cacagcggcc ctgacacttg
     2221 cacgatttgt cgcatatgca cggccgaacc actttattgc ctcatccagc tggccaaagc
     2281 cgtacttcct ggtgccaatt atcgtataga tccttgccct ggagatatag acaagtatca
     2341 aaaaatacac caacagcgcc gcaatgccga gccacttgtt gatccttgcg gcgattatga
     2401 cgccgataac gggcagggca aaacgggtca gaaggattct cgtattactg ttcattatgt
     2461 tgttgttcct gttatcgctc atcatggacc ctccgaatca ggacaatatt cgatatcgat
     2521 atcctctatc cgcggctttg catgctgcgt gatcaagtca attataaacc atcgctgcac
     2581 aggattgcaa atgaccggga catccggaca tttgaggcat ggccttcctg tatatggggc
     2641 cgtggctcgg tgtcggctgt tttttgcata agccttgcgc agcttctttt gtatttgcct
     2701 ttcgtgtatt tacctttctg cgatttgaat a
//
LOCUS       DAOSCC010000269         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24173_length_2722_cov_2.870641, whole genome shotgun sequence.
ACCESSION   DAOSCC010000269 DAOSCC010000000
VERSION     DAOSCC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24173_length_2722_cov_2.870641"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PLG72_10915"
     CDS             <1..1441
                     /locus_tag="PLG72_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ81346.1"
                     /translation="KIPALSREKEWDFVPVKKAGDAVQAGDIIGTVQENEVFQHRIMV
                     PYGVEGILESIGSGKFTIEQIIARVRDKNGNIRELTMMQKWPVRKERPYREKLPPDTP
                     LITGQRIIDTLFPIAKGGVAAIPGPFGSGKTVVQHQLAKWAEADLVVYIGCGERGNEM
                     TDVLMEFPELKDPKTGHPLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSV
                     ALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLASRLAQFYERAGKVKTLGADNRVG
                     SISAIGAVSPPGGDISEPVSQATLRIVKVFWGLDYNLSYKRHFPAINWLQSYSLYQDK
                     LSDWYRENISREWNRLRTDTMRILQEEAGLQEIVQLVGIDALSPADRVTLEAARSIRE
                     DYLQQDAFNEDDTYTTLEKQYRMLRIILAFYEKSQEAVKKGVDINDLFAIPVRERISR
                     MKLIPQKEGSRPFDEIEAELSRQIEALYK"
     gene            1592..>2722
                     /locus_tag="PLG72_10920"
     CDS             1592..>2722
                     /locus_tag="PLG72_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ81347.1"
                     /translation="MLKEYRSIVEVAGPLMLVRNVEGVTYNELGEIELGNGEIRRCRV
                     LEVDGNTALVQLFENAVGINLSTSKVRFLGRNLELPVSEEMLGRVFDGLGKPIDGGPA
                     IIPEKRLDINGLPMNPTARDYPNEFIQTGVSAIDGLNTLVRGQKLPIFSASGLPHADL
                     AAQIARQAKVIGTDSKFAVVFAAVGITFEEADFFVSDFKRTGAIERAVLFMNLANDPA
                     VERIATPRMALTAAEYLAFEKDMHVLVIITDITNYAEALREVSAARKEVPGRRGYPGY
                     LYTDLATLYERAGRMKDKKGSITLIPILTMPEDDKTHPIPDLTGYITEGQIILSRELH
                     KKGLTPPIDVLPSLSRLKDKGIGKGKTREDHANTMNQLFAAYA"
BASE COUNT          776 a          633 c          710 g          603 t
ORIGIN      
        1 caaaatccct gcattgagca gggaaaaaga atgggatttc gttccggtaa aaaaagccgg
       61 ggatgcggtc caggccggcg acatcatagg aaccgtccag gaaaacgagg ttttccagca
      121 caggatcatg gttccatatg gcgtggaagg aattcttgaa tcaatcggat ccggaaagtt
      181 caccatagag cagatcatcg ccagggtcag ggataaaaac ggcaatatca gagaactgac
      241 catgatgcag aaatggccgg tccgtaaaga aagaccatac agggagaagc tgccgcctga
      301 tacaccgctt atcacaggac aaagaataat cgacaccctc ttcccgatcg caaaaggagg
      361 agttgctgcg attcccggtc ctttcggaag tggcaagacc gttgtgcagc atcagctcgc
      421 aaaatgggca gaagccgatc tggtggtcta tataggttgc ggtgagcgcg gtaatgaaat
      481 gacagacgtt cttatggagt tcccggaact gaaggaccca aaaaccggac atccccttat
      541 gaaaagaaca gtacttatag ccaacacctc tgacatgcct gttgctgccc gcgaagcttc
      601 gatctataca ggaataacca tagccgagta cttcagggat atgggttatt cggtggctct
      661 gatggcagac tctacatccc gctgggctga agctctccgc gaaatgtcag gacgattgga
      721 ggaaatgccg ggtgaggaag gttatccggc ttatctggca tcacgtcttg cccagttcta
      781 tgaaagggcc ggtaaggtaa aaacccttgg agcagataac agggtcggtt caataagtgc
      841 cataggagcc gtatcacctc ccggcggcga tatctcggaa cctgtgtcac aggcaacctt
      901 aagaatagtt aaggttttct ggggcctcga ctataatctg tcctacaaga gacacttccc
      961 cgccataaac tggctgcaga gctattccct ctatcaggac aagctttcag actggtatcg
     1021 tgagaacata tcaagggaat ggaacagact gaggactgat acgatgcgta tcctccagga
     1081 ggaggcaggg cttcaggaaa tcgtacagct ggttggtatc gatgcgctct ctcctgcaga
     1141 cagagtgacc cttgaggctg caagatccat acgtgaggac tatctgcagc aggatgcatt
     1201 caacgaagat gacacctata cgacacttga aaaacagtac agaatgctga ggatcattct
     1261 ggcattctat gagaaatctc aggaggctgt taagaagggt gttgatataa acgacctctt
     1321 tgcaatacca gtaagagaaa ggatcagccg tatgaagctg atcccgcaga aagagggaag
     1381 caggcctttt gacgagatag aggcagagct gtccagacag atcgaagcac tctataaata
     1441 aatatatggt tatctgctgc ggaaacagat ctcaactact ataaataact ttggtgcaaa
     1501 ttgaaggatt caatttgcga cgtaccaaaa ttcgaatgaa ttatgtctga tgcgaacctc
     1561 aggttcgcgt taggaaggag agaagagctt aatgcttaag gaatatcgtt caatagttga
     1621 agttgccggt ccgttgatgc tggttcgtaa tgttgaaggc gttacttata acgaactggg
     1681 tgagatagag cttggcaacg gagaaatcag acgctgcagg gttctggagg tagacgggaa
     1741 caccgcgctg gtacagctct ttgaaaacgc cgttggcatt aacttatcca ccagcaaggt
     1801 tcgcttcctg ggacgcaatc ttgaactccc ggtttcggag gaaatgctgg gaagggtatt
     1861 cgacggactt ggaaagccta tcgacggcgg accggccatt attcctgaaa aaaggctgga
     1921 cataaacggc ctgcctatga acccgactgc gagagattat cccaatgagt ttatccagac
     1981 cggcgtatcg gctatagacg ggctcaatac ccttgtaaga ggtcagaagc ttcctatctt
     2041 ctcggcttca ggtctgccgc atgcagatct ggcagcacaa atagcccgtc aggcaaaggt
     2101 tatcggaaca gacagcaaat tcgccgttgt gtttgcggca gttggaataa cctttgaaga
     2161 agctgacttc tttgtcagtg acttcaagag aacaggcgct atcgaaagag cggtactgtt
     2221 catgaatctt gccaatgacc cggcagtcga gcgtatcgca actcctcgta tggctctgac
     2281 agccgctgaa tatctggcat tcgaaaaaga tatgcacgta ctggtaatca taactgacat
     2341 aacaaattac gccgaagctc tgcgtgaagt atcggcagcc cgcaaggagg ttccggggcg
     2401 ccgcggctat cccggttatc tgtataccga cctggctaca ttgtatgaac gcgcaggccg
     2461 catgaaggac aagaaaggtt cgataactct tatccccatc cttacgatgc ccgaggatga
     2521 caagactcac cccatccctg acctcaccgg ttatatcaca gaagggcaga tcatattgag
     2581 ccgtgaactc cacaagaaag gccttactcc tcccatcgac gttctgcctt cactttcacg
     2641 tctgaaggat aagggaatag ggaaagggaa aacccgtgaa gatcacgcca atacaatgaa
     2701 ccagttgttt gccgcatatg ca
//
LOCUS       DAOSCC010000270         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24227_length_2718_cov_4.475028, whole genome shotgun sequence.
ACCESSION   DAOSCC010000270 DAOSCC010000000
VERSION     DAOSCC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24227_length_2718_cov_4.475028"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLG72_10925"
     CDS             complement(<1..936)
                     /locus_tag="PLG72_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ81348.1"
                     /translation="MVTLKQISQICGLSVSTVSKAMNGQPDVNPATAERIRQVARDLG
                     YRPNAAARSLKTKRSYLIGVLFADQINHEYFANVLNSIRNTATDLGYDIMFISDRVGS
                     SKMSFYSHSAYRNCDGVIIMEARFEDPDVVELAASDLPIVVLDHVFSGCVSVMTDNVE
                     SMVEIIHFVVSRGHRKIAFIHGQENGFITQKRIAGFYKGCMELGIRTPEEYIVSGKYH
                     DPEASAEATEMLLSLPEPPTCIIYPDDYSYLGGLRIFEKHGLSVPDDISAVGFDGINL
                     SIHLRPQLATYRQDAHMIGKQAVIKLIEQIEGPMKK"
     gene            complement(1595..>2718)
                     /locus_tag="PLG72_10930"
     CDS             complement(1595..>2718)
                     /locus_tag="PLG72_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOJ81349.1"
                     /translation="NPVDDSFLKALDDFSYKTGALVLADKGKNINYSPLSLYYALSVA
                     AAGAGSDTRDQLLALLGVSDPQVLAEQCGNLYRRVYRDNKIGKLKIANSIWLDDDMDG
                     EPVSFKGSFVKNAAENFYASCHSVDFSGKETGKAMADWVSANANGTLSPTIETDPEQI
                     LSILNTVYFYDQWIDRFNRSKTAKDMFYLSNGDEVEVDFMNKTNPSTGFSRGEGFTRS
                     GMSLKNGSHMEFILPDEGVSPYDLLASPDRMKETFEGGESFVGEVVWKIPKFSFGSRL
                     ALTDALKSLGVTSAFLPDADFSGITDHMAFITDVLQETHMAIDEDGVEASAFTQINYA
                     GSALPEGRAEMILDRPFIFCITAPDGSMLFVGVCENPAE"
BASE COUNT          691 a          697 c          607 g          723 t
ORIGIN      
        1 ttttttcatc ggcccttcga tctgttctat cagctttatt actgcctgtt tccctatcat
       61 atgggcatcc tggcgatatg tggcaagctg cggccgcaga tgtattgaaa ggttgatgcc
      121 gtcgaatccg acagcactta tgtcatccgg aacggagagg ccgtgtttct cgaagattct
      181 cagccctcca agatacgagt aatcatcagg gtaaatgata catgtcggag gctccggaag
      241 ggataaaagc atttccgttg cttcggcgct tgcctccggg tcgtggtatt ttccactgac
      301 tatatattct tcaggcgtcc ggatgcccag ttccatacag cccttgtaga atccggcgat
      361 acgtttctgc gtaatgaaac cgttctcctg gccgtggatg aaagctatct tccggtgtcc
      421 cctgctgacg acaaaatgta ttatttcgac catgctctcc acattatccg tcatgaccga
      481 tacacacccg gaaaaaacat ggtccagtac aacgataggc aaatcactgg ctgcaagttc
      541 gacgacgtca ggatcctcga accgtgcctc catgattatg acgccgtcac agttccggta
      601 ggcagaatgg ctgtaaaagc tcattttcga actgcctacg cggtcgctga tgaacattat
      661 gtcatatccc aggtctgttg cagtgttgcg gatgctgtta agaacatttg caaagtattc
      721 atggtttatc tgatcggcaa acaaaacgcc tataaggtat gaccgtttag tcttgagaga
      781 acgtgcggct gcgttcgggc gatatcccag atcacgcgcc acctgtctga tacgctctgc
      841 cgttgccgga ttgacgtccg gttggccatt cattgctttc gacactgtag aaaccgatag
      901 accgcatatc tgcgatatct gttttagcgt taccaaggta agcctccgtg tccgaaatcg
      961 ttttcgtaat attataataa attgttataa gtgcgattgt caaccccaag ttaagcgcca
     1021 tattcaataa aatttcacct tattcgaaac gttttcgaaa tatatggctt catctcccct
     1081 tttccgttat aatttttatt gaaaagaaat ccccatcgac atacagatca acggccggtc
     1141 gtgcaagctc gccggaaagc catacacggc ctcagatgac ggcatatgca cacgcgtcaa
     1201 cctctacaac gaatgggtcc cggaagtccc cggcgggaca tgcaccgccg acggaagcgc
     1261 catgggggca tcggccatgc tgctcgatcc caaagactac ccggaaataa ggaccatgtc
     1321 cgtcacattc agatttgtcc ggcacagcag cctgcctcaa tgaacccacg ttccataaca
     1381 gccgcataga aaagcaaaaa ccctgaagat tctgattctt caggggtttt tatattacgg
     1441 agcaatcttg cagggttttt gccatagtca cagtggtcct tgccggactt aagccaagat
     1501 cagagtaatt tcaccggact gccggaaacc ttaatatata acgttccaat gacaattgca
     1561 atgacctttt ggccgtaaaa caaggccata tttattactc ggccgggttt tcacacaccc
     1621 cgacaaacag catgctgccg tcaggtgcgg tgatacagaa gataaaagga cggtcaagga
     1681 tcatttctgc gcggccttca gggagcgcag atcctgcata attgatctgg gtaaatgcgg
     1741 atgcttcaac accatcttca tcaattgcca tatgagtttc ctggagcacg tcagttataa
     1801 atgccatgtg gtccgtaatt ccgctgaagt ctgcgtcagg cagaaaggca gaggttaccc
     1861 caaggctttt gagggcatcg gtcaatgcaa gtcttgagcc gaaactaaac ttgggtattt
     1921 tccatactac ttccccaacg aatgattctc ctccttcgaa agtctccttc attctatccg
     1981 gagacgcaag cagatcatac ggggaaacgc cttcgtcagg cagtatgaat tccatatggc
     2041 ttccgttttt cagcgacatg ccggacctgg taaaaccctc tcccctgctg aatccggttg
     2101 atggattagt tttattcata aaatcaactt ccacctcatc gccattcgag agataaaaca
     2161 tatcttttgc agttttgctc ctgttaaaac ggtcgatcca ttgatcgtaa aaataaaccg
     2221 tgttcagtat tgacaggatt tgctcaggat ctgtctcaat tgtcggcgaa agcgttccgt
     2281 tagcgttggc cgatacccag tcagccatag cctttcccgt ttcttttccg gaaaaatcga
     2341 cactgtggca gcttgcgtag aagttctctg ccgcattttt tacaaaactc cctttgaatg
     2401 aaaccggttc accatccata tcatcatcaa gccagatcga gtttgcaatt ttaagctttc
     2461 cgattttatt atccctgtaa acccttctgt aaaggttgcc gcactgctct gcaagcacct
     2521 ggggatcgga aacgcctaac agggcaagca actgatcccg cgtgtcgctg ccggcgcccg
     2581 cagctgcgac agacagggca taatacaacg acagcggcga atagttgatg tttttgcctt
     2641 tatctgcgag cacaagggcc cccgttttat acgaaaagtc atcaagggct tttaaaaagc
     2701 tgtcatcaac agggtttt
//
LOCUS       DAOSCC010000271         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24572_length_2692_cov_4.162306, whole genome shotgun sequence.
ACCESSION   DAOSCC010000271 DAOSCC010000000
VERSION     DAOSCC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24572_length_2692_cov_4.162306"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PLG72_10935"
     CDS             <1..824
                     /locus_tag="PLG72_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HOJ81350.1"
                     /translation="GYRKLGYQRKGIPPRLAVRRSVVEKIKRKQSYKRTLAELKKERG
                     EDGDVICGDTNTCEEAGMPDAAACNVIGRFTFAEEDLRYRRLREIRKKDFNAVVICIM
                     DVSGSMDQTKKYMARSFYFLLYQFLRHKYSNVEVVFIAHTTTAKVVNELEFFHRGESG
                     GTYISSGYEKALEMIEQHYNPANWNIYVFHCSDGDNWIEDNERAVEYAKKLCEKCNLF
                     GYGEIVPGYYFSGSTVRTEFINNIQADNFIIVTMSRKEDIITALKKLLAKDGDKQ"
     gene            987..2372
                     /locus_tag="PLG72_10940"
     CDS             987..2372
                     /locus_tag="PLG72_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOJ81351.1"
                     /translation="MADYSISELEMWSKRIEEIAAEAGLDCYEQEFELVSYEEMTSYE
                     AYSGMPSHYPHWSYGKAYERIRTLNRYNLSGLPYEIVINSNPCIAYLMKDNSLLLQIL
                     TMAHVYAHNDFFKNNRLFKAGTRAEHTVGMFKNHAERVREYIRDPSIGYLKVEKVLDA
                     AHALRYQCERTIGVKKLTDDELRERLKNRNKKTAGDFPLLEPKPTYTDEPDLRRLPLE
                     PEEDLLYFIGKYGRLEPWQKDIIDIVREETRYFIPQIETKIMNEGWASFWHYMILNRL
                     ELPQKLHFEFLRRHNQIIRPYEGRINPYFLGFKIFMDLYEKNDRKTGKIFEIRTTERD
                     DTFIRRYLTKELCVEMKLFEFEKHGSEYVVSEVADDKGWTKIRDTIASSLGFGSLPVV
                     RVIDWNQKENTLLLEHLYDGRELDLQYANETLKHLIDLWEGKVILNTVVEGKKRSIVC
                     DEQRKILMINT"
     gene            2513..>2692
                     /locus_tag="PLG72_10945"
     CDS             2513..>2692
                     /locus_tag="PLG72_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81352.1"
                     /translation="MKRYCCFILSALITSLFLLTGCNSDAGGRTGDEHTADAEEAANA
                     PNISASTNLEGDILFY"
BASE COUNT          837 a          559 c          713 g          583 t
ORIGIN      
        1 aaggttacag gaagttggga tatcaaagga aaggcatccc tccgaggctg gcggtcaggc
       61 gatcggtcgt agagaagatc aagcggaagc agtcatacaa aaggaccctc gcggaattga
      121 aaaaagagcg gggtgaagat ggagacgtta tttgcgggga caccaacaca tgtgaagaag
      181 cagggatgcc ggatgctgct gcatgtaacg taatcggcag gtttacgttt gccgaggaag
      241 acctccggta caggaggctt cgtgagataa ggaaaaaaga cttcaatgca gtcgtgatct
      301 gcatcatgga tgtctccggg tccatggacc agaccaaaaa gtacatggcc cgcagttttt
      361 acttcctgct gtaccagttt ctaaggcata agtattcgaa tgttgaagtg gttttcattg
      421 cgcacaccac cactgcgaag gtggtaaacg aacttgagtt tttccacaga ggcgagtcgg
      481 gcggcaccta tataagcagc ggttatgaaa aagcccttga aatgatcgaa cagcactaca
      541 atcctgccaa ctggaacatt tacgtcttcc actgcagcga cggtgacaac tggatagaag
      601 acaatgagcg ggctgtagag tatgccaaaa agctatgcga gaagtgcaat cttttcggat
      661 acggggagat agtgcccggc tattacttct ccggcagcac tgtgaggacg gaatttatca
      721 acaacatcca ggcagacaac ttcataatcg taacaatgtc aaggaaagaa gacataataa
      781 cggcgttgaa aaagctactg gccaaagacg gcgacaagca gtaaacgccg gagtattccc
      841 aagaagctgc taacgcatca gataaagtga accggacgtc cggatcaaag caggccggtc
      901 aaaagaacgg aacgggaact gccggatcga atcaacctga acaagcaaca tcatgcgttt
      961 ccaaccaata tgacagggag cgggatatgg cggattacag cataagcgaa ctggaaatgt
     1021 ggagcaaaag aatagaagag atagcagcag aagcagggct ggactgctat gagcaggagt
     1081 tcgagcttgt cagctatgaa gagatgacgt cttacgaagc atattcgggc atgccgtccc
     1141 attaccctca ctggagctat ggcaaagctt atgaacgcat aaggaccctg aacaggtaca
     1201 acttgtcggg attgccttat gaaatagtga taaactcgaa tccctgcata gcttatctga
     1261 tgaaggacaa ctcgcttctt ttgcagatcc tgaccatggc ccacgtttat gcccacaatg
     1321 attttttcaa aaacaacagg ctgttcaaag cagggaccag ggccgagcac acggtcggga
     1381 tgttcaagaa tcacgcggag agggtaaggg aatatataag ggatccgagc atcggctatc
     1441 tgaaagttga aaaagtgctg gacgcagccc atgcgctccg gtatcagtgt gaaaggacca
     1501 taggcgtaaa gaaacttacg gacgatgaac tcagggaaag gctgaaaaac aggaataaaa
     1561 agactgccgg cgatttccct ctgctcgaac cgaaaccgac atataccgat gaaccggacc
     1621 tgaggcggct tccgctggaa cctgaggaag acctgctgta ttttataggc aagtatggaa
     1681 ggttggaacc ctggcagaag gatattatag atatcgtcag agaggaaacg aggtatttca
     1741 ttccgcagat cgagacaaag atcatgaatg aagggtgggc aagcttctgg cactacatga
     1801 tcctcaacag gctggaactt ccccagaaac tccatttcga attcctcagg aggcacaacc
     1861 agataatccg gccgtacgaa ggcaggataa atccatattt cctgggcttt aaaattttta
     1921 tggaccttta tgaaaaaaat gaccgtaaaa caggcaagat attcgagatc aggacgacag
     1981 agagggacga tacctttatc aggagatatc ttacgaagga actgtgcgtg gagatgaagc
     2041 tgttcgagtt tgaaaaacac ggcagcgaat atgttgtttc ggaagttgcc gatgacaaag
     2101 gctggacgaa aataagggat acgatagctt cttcgttagg attcggaagt ttgcccgtcg
     2161 tcagggtaat cgactggaac caaaaggaga acacgctttt gctggagcat ttatatgacg
     2221 gaagggaact ggacctccag tatgccaacg agactttgaa gcatttgatt gatctctggg
     2281 aaggcaaggt aatattgaat acggtggtcg aaggcaaaaa aaggtcgatc gtgtgtgatg
     2341 agcaacggaa aatattgatg atcaatacct aatccatggc atttcttcag atcccggcaa
     2401 tctgacagtt atatgccgga tgccggtctg tcatatgtaa ttcaattgtg atagaataag
     2461 aatgaataaa ctgccagttt atatcagttt aacataagtg aggtctccac aaatgaaaag
     2521 gtactgttgt ttcatccttt cagccctgat aacgtcgttg tttcttctga caggctgcaa
     2581 ttcggatgcc ggcggaagaa ccggcgatga gcataccgct gatgcagagg aagcggccaa
     2641 tgcgccgaat ataagtgcgt caacgaatct cgaaggagac atactcttct at
//
LOCUS       DAOSCC010000272         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24737_length_2678_cov_4.075486, whole genome shotgun sequence.
ACCESSION   DAOSCC010000272 DAOSCC010000000
VERSION     DAOSCC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24737_length_2678_cov_4.075486"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..2643
                     /locus_tag="PLG72_10950"
     CDS             292..2643
                     /locus_tag="PLG72_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ81353.1"
                     /translation="MSIGSILLIGNIETQTVFYIIYSTLLLALLLAFAIRTYISRRDR
                     KLYLQREVSEAIIENAKTMVFIFSLDGKVLIFNKFAQEMTGYGRNDVVGHTIDDIPFL
                     RKDAELGKVIRDAISEKAIVHNREISFTAKNGRKIYSLWNIDMINDTGNVPVYVAAMG
                     IDITDRKNTESRLAESYQELESLYRELVTKEIELRLQFDDLNARDNDLRRSEERYRLA
                     VEGVNDGVWDWDGKDGRLFMSKKARAILGIESDEDFLTIEKWFDVILREDLDKFVVSL
                     NRYITEPQNKHLQIEYRIKAKNDEIKWIRTRGMAIWDEEGNPIRVAGSITDITEQKLA
                     DEKIHRLAFFDSLTGLPNRALLMDRFSVAEANARRKGKSIAVFFLDLDNFKTINDTLG
                     HTYGDKLLLKVGEQLRLKLRKSDTIARLGGDEFIMLQTNISSMDEVYKLAARMLNVFK
                     KPWKLDDREFYVTASIGISIYPNDGTDLRELMKNADVAMYRAKEKGKNNFQIFTPELN
                     MKIMERMEIENHLRKAAERKEFVLYYQPQIELATGKVRSVEALIRWSDPNMGWIMPEA
                     FIHIAEETGLIGDIGEWVMTTACTQLRKWHDEGHDDLRISVNLSARQFQQHDIFEKIM
                     DIVEKTAIRPEWLELEITESLAMQDLEHTTGILRRIRDAGFGISLDDFGKGYSSLNYL
                     KALPITNLKIDKTFIHGISNSANQAKIVKALISLAHSMDLTVTAEGVESSAQLEFLVK
                     ERCDTAQGYLFSKPKPAKDVDLSSYGALVEKHAESPQCEEMTS"
BASE COUNT          825 a          578 c          723 g          552 t
ORIGIN      
        1 atggaccagc tgaacgacct tatgaaatac ggatcatccg acagtccgca ggacctgggg
       61 ggataccatg actacaagga attctattcc aatgaaactg acaggcaggg aatagttgac
      121 gacatggacc ggatctccaa tgatgattac aggagacaac tgcccgatta ataaggcaat
      181 attatattaa gtttacagta attgcagaaa tatattggaa agaggtaaaa aaaattatat
      241 aatcaaattg aaagacattt tgcaaagtct ttatttgttt tagggggaaa aatgagtata
      301 gggtcaatat tactcattgg gaatattgaa acgcaaacgg tcttctatat catatattct
      361 acacttctgc tggccctgct gcttgctttc gcgatacgca cctatatttc gagacgcgac
      421 aggaagctgt acctgcagcg tgaagtttcc gaagcgataa tcgaaaatgc aaaaacgatg
      481 gtttttattt tttccctgga cgggaaagtg ctgatcttca acaaatttgc acaggagatg
      541 acgggatacg ggagaaatga tgtagttggt cacaccatcg atgatatacc tttcctccgc
      601 aaggatgccg aacttggaaa ggtgatccgc gacgccatat cggaaaaagc gatcgtgcat
      661 aacagggaaa taagctttac tgcaaaaaac ggaaggaaga tctactccct ctggaacata
      721 gacatgatca atgacaccgg aaatgtccct gtatatgtgg ccgcgatggg gatcgacatc
      781 acggaccgca agaatacgga atcgaggctt gccgaaagtt accaggaact cgaatcgctg
      841 taccgggaac tggtgacaaa ggaaatagaa ctaaggctcc agtttgacga tctgaatgcg
      901 agggacaatg atctgagacg aagcgaggaa cgttacaggc tggcggtcga aggggtgaat
      961 gacggcgtct gggactggga cggcaaggac ggaaggcttt tcatgtcgaa aaaggcgcgg
     1021 gccattctcg ggatagaatc ggacgaagac ttcctgacga tagagaaatg gttcgacgtg
     1081 atcttaaggg aagacctgga caaattcgtg gtaagcctga acaggtacat taccgaaccg
     1141 cagaacaaac acctccagat cgaatacagg ataaaagcga agaatgacga gataaagtgg
     1201 atcaggacaa ggggaatggc aatatgggat gaggaaggca atcccatcag ggtggccgga
     1261 tccatcacgg atataactga acagaagctt gccgatgaaa agatacacag gcttgctttc
     1321 ttcgattctt tgacgggact gccgaacagg gcgctgctga tggacaggtt cagcgttgcc
     1381 gaggcgaacg cccgcaggaa gggcaagagc atagctgtat ttttccttga cctggacaat
     1441 ttcaaaacca taaatgatac gctggggcat acttacggcg acaagctcct tctcaaggta
     1501 ggcgagcagc ttcggctcaa actgagaaaa agcgacacca tcgcaagact gggcggcgat
     1561 gaattcataa tgctccagac gaatataagc agtatggacg aagtgtacaa gcttgccgca
     1621 aggatgctca acgtattcaa aaagccgtgg aagcttgatg accgcgaatt ctatgtcact
     1681 gcgagcatag gcatatccat atacccgaat gacgggacgg atctcaggga actgatgaaa
     1741 aatgcggatg ttgccatgta ccgtgcaaaa gaaaagggaa agaacaattt ccagatcttc
     1801 actcctgaac tcaacatgaa gataatggag cggatggaga tagaaaacca tctgcgtaag
     1861 gcagctgaac gaaaagagtt cgtcctgtat taccagcccc agatagaact ggccacgggc
     1921 aaggtcagga gcgtggaggc actgataaga tggtcagatc cgaatatggg ctggatcatg
     1981 cctgaggcgt tcatacacat agcggaagag acaggcctga tcggggatat aggcgaatgg
     2041 gtgatgacca ctgcctgtac ccagctgcgc aaatggcacg acgaagggca tgacgacctc
     2101 aggatatctg tgaacctgtc ggcaaggcaa ttccagcagc atgatatttt tgaaaagatc
     2161 atggacatag tcgaaaaaac cgccatcagg ccggaatggc tggagcttga aataaccgaa
     2221 agcctggcca tgcaggatct cgagcatacc acgggcatcc tgagacgtat cagggatgcg
     2281 ggttttggca tatcgcttga cgacttcggg aaagggtatt cttccctcaa ttacctgaaa
     2341 gcgctgccga taacgaacct gaaaatagac aagacattca tacacggcat atcgaacagc
     2401 gcaaaccagg ccaagatcgt aaaggcactg atatcgctgg cccacagcat ggatcttacc
     2461 gtgactgcgg agggagtgga gagcagcgcc cagttggagt tcctggtgaa agagcgctgc
     2521 gacactgccc aggggtatct gttcagcaag ccgaagccgg cgaaagacgt agacctgtca
     2581 tcatacgggg cactggtgga aaagcatgcg gaatcgcccc agtgtgagga aatgacttcc
     2641 tgaccatacc taaccgactt cacggccgga ctttctta
//
LOCUS       DAOSCC010000273         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_24766_length_2676_cov_4.474246, whole genome shotgun sequence.
ACCESSION   DAOSCC010000273 DAOSCC010000000
VERSION     DAOSCC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24766_length_2676_cov_4.474246"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLG72_10955"
     CDS             <1..367
                     /locus_tag="PLG72_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HOJ81354.1"
                     /translation="LVEIIKPSSERVTPACPVFGTCGGCQLQHLNYQAQLLMKQQIVY
                     DVLKRIGGLKNIEVQPVLGMAEPWGYRNKGHFRVENRDGGIKIGFFEEESHKQTDECC
                     KYLFSRKVVDSLDRLPGML"
     gene            416..643
                     /locus_tag="PLG72_10960"
     CDS             416..643
                     /locus_tag="PLG72_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81355.1"
                     /translation="MLIRESHSRGKLLLIFVISEKMDYDFSEIAREICKNNPDVVGVC
                     TNINPMKAGPVLGEKTKVIFGKGEIEDKIGP"
     gene            complement(849..1898)
                     /locus_tag="PLG72_10965"
     CDS             complement(849..1898)
                     /locus_tag="PLG72_10965"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOJ81356.1"
                     /translation="MYMNAAPSVKYEIIRATISRDNNLLNISELCKLAGVSRSGYYNW
                     VACEKKRKELEEQDRADFELILLAFQYRGYDKGVRGIHMRLLHQKPPVVMNPKKIRRL
                     MAKYNLRCPIRKPNPYRRISKAMQTNNVAPNVLNRQFKAFGPRTVLLTDITYIPRYSH
                     HAGGSLKYTYVSVIMDAFTKQVLACVCSTSLEVDFVLDTVKQLINNHGGELRTDTLIH
                     SDQGCHYTSTKFVEILGNYELRRSMSRRGNCWDNAPQESLFGHMKDEIRLLNSDAHQQ
                     IQRKVLDWIDYYNNERYQWSLAKLSPNEYYEYIKTGVYPLSCISDECNVRGSAPDPPE
                     FNALVSGKGNEKDDA"
     gene            complement(1901..2425)
                     /locus_tag="PLG72_10970"
     CDS             complement(1901..2425)
                     /locus_tag="PLG72_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81357.1"
                     /translation="MAKDGTNRGGARPGAGRPKKRDDGKYEHVELTAEQIHELLDSPH
                     ISYVSRKSVSYTKAFKEHFWQRYCDGVDPKQIFEDAGLNTAILTRSRINGLLKTLRHQ
                     KEKGLAFTEGSEPQVDQPEKTYDFPKPPRRSNKSKLPVLSEADIAQMYNQVAYMSQEI
                     EFLKKIILAGQEKK"
BASE COUNT          621 a          610 c          688 g          757 t
ORIGIN      
        1 tttggttgaa ataatcaagc catccagtga acgggttacg ccggcatgcc ctgtttttgg
       61 cacttgcggc ggctgccagt tgcagcatct taattaccag gctcagttac tgatgaagca
      121 gcagatagta tatgatgtct taaagcgaat cggaggctta aagaatatag aggtacaacc
      181 ggtactgggc atggcggagc cgtggggata ccgcaataaa ggacatttca gggtagagaa
      241 cagggatggc gggattaaga taggcttttt tgaggaagaa agccataagc agacagatga
      301 gtgttgcaaa tacctttttt ctcgtaaggt tgtggattcg ctggaccggc tgcctgggat
      361 gctgtaagac tttaaggtgc ctgtagcgac tggcggacag ccgggactca agaatttgct
      421 gatcagagaa agccacagca ggggtaaact actgcttatt ttcgttattt cggaaaaaat
      481 ggactatgat ttcagcgaaa ttgcacggga aatatgtaag aataatcctg acgttgtcgg
      541 tgtatgtaca aatattaatc ccatgaaggc agggcctgtc ctgggtgaga agacgaaagt
      601 tatctttggc aagggtgaaa ttgaggataa gattggtcct taaaactaaa gaattactta
      661 acttttgaga ttttctgcag tttctggggt agggtgaagt gctcccctca gcgtggacaa
      721 catgagcaag ctttgaaacc gagtcctcat tccatggtat aatgtaaatg ctttaccaat
      781 acgcaggata gggcaacgct ggagagcgac ctgagcagcg tattgggttt gcggggaagg
      841 cgatgtcgtc aggcatcgtc tttttcattg cctttcccgg aaaccaaagc gttaaactcc
      901 ggggggtcgg gggcggagcc cctgacattg cattcatcgc ttatgcagga taacggatac
      961 acccctgttt ttatgtactc atagtactcg ttcggtgaaa gctttgcaag cgaccattgg
     1021 taacgttcgt tgttgtagta atcgatccaa tccagcactt tcctttgaat ctgctgatga
     1081 gcgtcgctgt taagcaggcg tatttcgtcc ttcatgtgcc cgaataagct ctcctgcggg
     1141 gcgttgtccc aacagttgcc ccggcgggac atcgatctgc gcagctcata gttccccagt
     1201 atctcaacaa acttggtgct tgtgtaatgg cagccttgat ccgaatgtat cagcgtatcg
     1261 gtgcgaagct cgccgccatg gttatttatc agctgtttga ccgtatcaag aacaaaatcc
     1321 acctcaaggg aagtactgca tacgcaggcc agcacctgct tcgtaaacgc atccatgatc
     1381 accgaaacat aggtgtactt cagagagccg cccgcatggt gggaatagcg aggtatgtag
     1441 gttatatctg tcagcaacac tgttctcggg ccaaaagcct tgaattgccg gtttaaaaca
     1501 ttcggagcga cattgttcgt ctgcatagcc ttggaaatcc tgcgatatgg gttcggcttg
     1561 cggatgggac agcggaggtt gtactttgcc ataagccgcc gtattttctt tggattcata
     1621 acaaccggcg gcttctgatg cagcaagcgc atatgtatgc ccctgacgcc tttgtcatag
     1681 ccgcgatact gaaatgccag cagaatcagc tcaaaatccg ctcgatcctg ctcttcaagc
     1741 tccttccgtt tcttttcgca agcgacccag ttgtagtagc cggagcggga aacgccggcg
     1801 agcttgcaca actcggaaat gttgagaaga ttgttgtcgc ggctgatcgt cgcgcgtatt
     1861 atctcatatt ttactgaagg tgctgcgttc atatacatgg ctactttttc tcctgtcccg
     1921 ccaagataat tttttttaga aactctatct cctgggacat gtatgcaacc tgattgtaca
     1981 tttgcgcaat gtcggcctcg cttaacaccg gcagcttcga tttgttggat ctgcgcggcg
     2041 gtttgggaaa gtcgtaggtc ttttcaggct gatcgacctg cggctcgctg ccctcggtaa
     2101 aggcaagccc tttctctttc tgatgcctga gtgtttttag aagaccattg atcctgcttc
     2161 ttgtcaggat cgccgtgttc agcccggcat cttcaaatat ctgtttcggg tcgacgccat
     2221 cgcagtatct ttgccagaaa tgctccttga aagcctttgt atacgatacg gacttccttg
     2281 aaacatagct gatatgcggc gagtccagca gctcatgaat ttgttcggcg gtgagttcga
     2341 catgctcata cttcccgtcg tcccttttct ttgggcggcc tgctccggga cgcgcgccgc
     2401 ccctgtttgt accatccttt gccattgaaa tcctcctttt ggagagcgaa gaagtcgttt
     2461 gaattatcat agcatgattt ggtgtccatt ttatgaattc caaacttttt tatctgtcca
     2521 tgatattgat ttcctcactc ggttatttgt ccagcgtttt gatttctcgt ttccaaattc
     2581 atttcaatgg ttgtccattt tttagggtgc atatcagggg cggtcaaaat ctctggtgga
     2641 tatgtttcgt gcaacgggcg gggggtcacg cgcgaa
//
LOCUS       DAOSCC010000274         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_25133_length_2649_cov_4.178874, whole genome shotgun sequence.
ACCESSION   DAOSCC010000274 DAOSCC010000000
VERSION     DAOSCC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25133_length_2649_cov_4.178874"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLG72_10975"
     CDS             complement(<1..268)
                     /locus_tag="PLG72_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HOJ81358.1"
                     /translation="MFTVKLKTLMEEFHLEPVCMSESAGDIEITTSDVNRPGLQLSGY
                     MEYFGTDRIQIIGKVEMTYLASLSPQERKKRLDDYFRTGFPCLVI"
     gene            complement(920..1609)
                     /locus_tag="PLG72_10980"
     CDS             complement(920..1609)
                     /locus_tag="PLG72_10980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ81359.1"
                     /translation="MARMNVNPTRMVLTGLKKRLSTARRGHKLLKDKRDELMKKFLEI
                     VRENKALREEAEEKLMKVHSRFVMARAGMSTEVMEEALMFPKQKVELKASTRNIMSVD
                     VPVLEFHAVSDDEFDIYPYGFADTSSELDAAIATLSELTPTLLKLAELEKSAQLLADE
                     IEKTRRRVNALEHVLIPNLEETIRYIVMKLDENERGNLTRLMKVKDMMLEQAHHYREK
                     YMAGQGPDVPH"
     gene            complement(1777..>2649)
                     /locus_tag="PLG72_10985"
     CDS             complement(1777..>2649)
                     /locus_tag="PLG72_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ81360.1"
                     /translation="GTDSKFAVVFGAIGITFEEADFFISDFKRTGAIDRTVLFMNLAN
                     DPAIERISTPRMALSAAEYLAFEKGMHVLVILTDITNYAEALREVSAARKEVPGRRGY
                     PGYLYTDLATLYERAGRKKGYEGSITLIPILSMPEDDKTHPIPDLTGYITEGQIILSR
                     ELHKKGITPPIDVLPSLSRLKDKGIGEGKTREDHANTMNQLFAAYARGKEAKELAVIL
                     GDAALTDIDKLYARFADAFESEYVSQGYDTDRTITETLDLGWKLLSILPVGELKRIKQ
                     EYIDKYLPANKYGE"
BASE COUNT          575 a          698 c          635 g          741 t
ORIGIN      
        1 cgatgacgag gcaggggaat cccgtgcgga aataatcgtc cagcctcttt ttcctttcct
       61 ggggagacag gcttgcaagg tatgtcattt ccaccttgcc tatgatctgg atcctgtctg
      121 tgccgaaata ctccatgtag cccgaaagct gaaggccggg cctgttcacg tcggatgtgg
      181 tgatctcgat atcacctgca gattccgaca tgcagaccgg ttccagatgg aattcctcca
      241 tgagcgtttt gagctttaca gtaaacatca gccgccctcc gtacaatgct taatgtatat
      301 aacagcaaaa catgctgcat gatacactgt gacgtgcata aaaaattatc ttattctgaa
      361 acacagttat cagttagatt atataccatc atgacagaat ttacgagagc gatatgacag
      421 atgccgtgca aagcctctga tgtaaagatt tggtcccggc atccggtccg aaagaagatt
      481 gcccgggttt gacaaagatt cctataggag ggtgtacaaa attttaatta ttgtgtggat
      541 ttttcagctt ataagcaaga aaagctgtgt catagcttta cataagtcat gataacgatg
      601 tattatgtag tctgataact ggaaaaacat gcaaatatta ttacaatagt cgattttttg
      661 tacacccgga tccttatttc ccggggaaat aacgtgttcg gtacaggctc cgtgatcaaa
      721 accgtaacag aagcaaacat gcacaatacc gtgaaatgat ttggcctggt cctgagacca
      781 ggccgaagtt aaaggctgaa cccttaagaa aatgtgatcc ggaaaatatc ctgcatttaa
      841 agcactgtgc actgtaaatg tttatgcact taaatctcca ggatgctccc gaagaccttc
      901 aggcggaaca caccccggct caatgcggca catccggtcc ttgtcccgcc atgtacttct
      961 ccctgtaatg gtgggcctgc tcaagcatca tgtccttgac cttcatgagc cttgtcaggt
     1021 tgcctctctc gttctcgtcc agtttcatga cgatatatct gatggtttcc tcgaggttcg
     1081 gaatcaggac atgctccagc gcattgaccc tgcgcctcgt cttttctatc tcatccgcca
     1141 gcagctgcgc agatttctca agctccgcca gcttcagaag cgtcggcgtc agttcggaga
     1201 gagtcgctat cgctgcgtcc agttcgctcg atgtatccgc aaagccatag ggatatatat
     1261 cgaattcgtc gtcggagacg gcatggaact ccagcacggg cacgtcgacg ctcatgatgt
     1321 tcctggtaga cgctttcagt tccaccttct gcttcgggaa catgagagcc tcctccatga
     1381 cctccgtgct catcccggct cttgccatga caaagcgcga atggactttc atgagcttct
     1441 cctcggcttc ctccctgagg gccttgtttt ccctgactat ctcgaggaat ttcttcataa
     1501 gctcgtcacg cttgtccttt agaagcttat gccctcttct tgcagtactt agcctttttt
     1561 tcaacccggt cagcaccatc cgggttgggt tgacattcat tcttgccatc ggctgttgct
     1621 cctctgactc gttttgttgg ttattgtccg caccatttta ctggctgcag atcccgttat
     1681 tttgtccctg gtgttgaacc cgtcgttttt cggccgccgc tttattactt ttatgacatt
     1741 aacccggctg cgattgcttt gaccttatat ccgccattac tccccgtatt tatttgcagg
     1801 caggtactta tcgatgtact cctgcttgat acgcttaagt tcgccgacag gcagtatcga
     1861 aagcagcttc catccgaggt cgagggtctc cgtgatcgtc ctgtcggtat cgtacccctg
     1921 ggaaacgtat tctgattcga atgcatcagc gaatctggcg tagagcttgt cgatgtcggt
     1981 cagcgcagca tctccgagta tgactgcaag ctccttggcc tccttgcccc tggcgtaagc
     2041 cgcgaaaagc tggttcatgg tattggcgtg atcttccctg gtcttgccct ctcctatacc
     2101 tttgtccttc agtcgggaca gcgacgggag cacgtcgatg ggcggagtga tgcccttctt
     2161 gtgcagttca cggctgagta tgatctggcc ttccgtgatg tatcccgtca ggtcgggaat
     2221 cggatgggtc ttgtcatctt caggcatcga aagtatcggt atcagcgtta tgctgccctc
     2281 atagcccttt ttgcggcctg cacgctcata gagcgtcgcc aggtccgtat acaggtaacc
     2341 gggatatccg cggcggcctg gcacttcctt gcgggctgcc gagacttcac ggagcgcttc
     2401 ggcatagttt gtaatgtcgg tgagtatgac aagcacatgc atgcctttct caaatgccag
     2461 gtattcggca gccgacaggg ccatacgcgg cgtcgatata cgttcgatgg ccgggtcgtt
     2521 ggccaggttc atgaacagga ccgtcctgtc tatggctcct gtcctcttga aatcgctgat
     2581 aaagaagtcg gcttcctcga atgtgatgcc tattgctccg aacaccaccg caaatttgct
     2641 gtcggtccc
//
LOCUS       DAOSCC010000275         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_25254_length_2640_cov_3.946228, whole genome shotgun sequence.
ACCESSION   DAOSCC010000275 DAOSCC010000000
VERSION     DAOSCC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25254_length_2640_cov_3.946228"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..>2640)
                     /locus_tag="PLG72_10990"
     CDS             complement(356..>2640)
                     /locus_tag="PLG72_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ81361.1"
                     /translation="KGNRSSYATISITAKVPDAPSSLRASVVSGSGITLLWNDNSNNE
                     TSFIIERYDDSQKKYVTIGTANADSPTYTDSTAVQGGTYIYRVFAYNGMGRSASPSNE
                     VTINAWDAVAPASLDVKPVSPTRLDLTWSYTGSDNYNTIIERKKGVDGTWTTIFTTAA
                     GVLKYSDTGLEPNTRYFYRVRKSLGTGVSGEPFPDESGHGAYTLLPTPTVSAKATSDD
                     TIYISWSGVSNADVVIERKMANGAFSPIMTVGPTVQGWYDNTGIVPGAFYTYRIMAKT
                     ATNYSLYSNEITVHSYYLGTPYGLTLSVRQNDEIELRWVDNSTDEAGFEIWRGAYGSG
                     QFGLYATVGKNVTTFIDSKVDRGVQYTYRVRAFSEGGSTYSEFSGTVSGGIGIINPPA
                     DLRYEYISEHQIKLRWTDTSDNEYGFIIEQKIGDNGSWNNIAWVSANQTEYIISYLNK
                     YMKYYYRVRTINYSGNADAVSEEILVFTALPGAPSDLQAVSMSSSQIKLKWNDNSDSE
                     KGFRVLRSLYSDRNFYPVAELVKDTTEYYDSGLSAGTRYYYKVEAYNDTGRSESNVAE
                     ARTNTRMYFSDIGGVPWAKDAIENLAGMGIIKGKSGTLFMPNDTVTRAEFAVMLVRAF
                     GLETAPVGSLADVRYDKWYYREVMIAENFGIVSGDGNNRFYPERPITREEIAVMVFRA
                     LQASRRKYNAHDNSVLEKFIDKNNISPGAVPAMAVLVGEGIMEGMQGSTLGPKYNATR
                     AQAAVFIYRALTKTEPGEAQ"
BASE COUNT          592 a          690 c          595 g          763 t
ORIGIN      
        1 ttgccaacag ggaacattac aagcatgtgg aaaaactgaa ggagcagtat tcgctttgag
       61 atttataaat accgaatcca aaatgcatgg tttccatgat cacagaactt tctgcatgaa
      121 ctgaagtgat caatatcaaa acgcaacgaa aagaagctgc agctttttat ttggctgcag
      181 ctttttcatc tttatttttc agcttattta gcagtatccg accaaatttt cttcagttac
      241 tgtcctttat ataacccatt tgaccggatc ccgggctata cagtgcttcc ggtccggctg
      301 cggagttccg gcaggttttt cggcccaatt ccagccttgc gggatctgat gcctgtcact
      361 gggcctcacc gggttcagtc ttcgtaagcg ccctgtaaat gaacacggct gcctgtgccc
      421 tggtcgcgtt atatttaggt cccaaagtgc tcccctgcat cccttccatg atgccttcac
      481 ccacgagcac ggccatcgcc ggtacggcac cgggcgaaat attgtttttg tctatgaatt
      541 tctcaaggac agagttgtca tgtgcattat atttgcgtct cgacgcctgg agcgcccgga
      601 ataccatcac tgctatttct tcccttgtta tcggcctctc gggatagaac ctgttgtttc
      661 cgtccccgga tactatgccg aaattctccg cgatcattac ttccctgtag taccatttgt
      721 catacctcac gtccgccagg cttccgacag gagcagtttc cagtccgaag gcccgcacga
      781 gcatgaccgc gaactcagcc ctggtcacgg tatcgttcgg catgaacagg gtaccgctct
      841 tgccttttat gatccccatg cctgccaggt tctcgatggc gtctttggcc catggaacgc
      901 cgccgatatc tgaaaaatac atcctggtgt ttgttctggc ttccgcaacg ttactttcgg
      961 agcgcccggt atcattgtac gcttcgactt tgtaataata gcgcgttccg gcactgaggc
     1021 ctgaatcgta atattccgtt gtgtctttca ccagttcggc aaccggatag aagttccggt
     1081 cgctgtacag gctgcgcagg acacggaagc ccttttcgga atctgaatta tcattccatt
     1141 ttaactttat ttgtgaagaa gacattgata ctgcctgtaa atcagaaggt gctccaggca
     1201 gcgccgtaaa tacgaggatc tcctcgctta ccgcatccgc atttcctgag tagttaattg
     1261 tcctgactct atagtaatat ttcatatact tattgagata cgatattata tattcggtct
     1321 gattggcaga tacccatgcg atgttgttcc agcttccgtt atcaccaatt ttctgttcta
     1381 tgataaagcc atattcgttg tctgaagtgt ccgtccagcg aagttttatc tgatgctccg
     1441 atatatattc gtatctcagg tctgcaggag gatttatgat ccctatacca cctgataccg
     1501 tgccggaaaa ctcagagtaa gtactacccc cttctgaaaa ggcccttacc ctgtaagtgt
     1561 actggactcc cctgtccact ttactgtcaa taaaggtggt gacgttcttg cctactgtgg
     1621 cgtacaatcc aaattgtccg cttccataag ctcctctcca tatttcaaaa ccagcttcat
     1681 cagttgaatt gtcgacccat ctcaactcta tctcatcgtt ctgcctgacg gataaagtca
     1741 gcccatatgg tgttccaaga taatagctgt gtaccgtgat ctcgtttgaa tataaggaat
     1801 aattggtggc ggtctttgcc attatcctgt atgtataaaa tgctccgggt acgatgcccg
     1861 tattgtcata ccagccctgt accgtcggcc cgacagtcat aatggggctg aaggccccat
     1921 tggccatctt cctctcgatc accacatcag cattgcttac gccagaccag gatatataaa
     1981 tagtgtcgtc cgacgtcgct ttggctgaga ccgtcggagt tgggagcaaa gtgtatgcgc
     2041 cgtggccgga ttcatcaggg aacggttcgc cggaaacgcc cgtcccgagc gacttcctta
     2101 cccggtagaa atacctggta ttgggctcaa ggccggtatc gctgtatttc agaacacccg
     2161 ccgcagttgt gaatatggtt gtccacgtcc cgtcaacacc ttttttgcgc tctatgatcg
     2221 tgttgtagtt atctgacccg gtatagctcc atgtgagatc caggcgtgta ggtgataccg
     2281 gctttacatc aagcgaagcc ggggccactg cgtcccaggc gtttatggtc acttcgttcg
     2341 aaggagaggc tgacctgccc atgccattat atgcaaaaac tctgtaaata tacgtgcctc
     2401 cctgtactgc tgtactgtcg gtatatgtag gggaatccgc attggccgtg cctatcgtga
     2461 cgtacttctt ctgactgtca tcataccttt ctataatgaa acttgtctca ttattggagt
     2521 tgtcattcca caaaagtgtt atccctgacc cggaaacgac actcgccctg agcgaagagg
     2581 gcgcatcggg gactttcgca gttatgctta ttgttgcgta tgacgaacgg ttccccttgt
//
LOCUS       DAOSCC010000276         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_25306_length_2636_cov_3.777606, whole genome shotgun sequence.
ACCESSION   DAOSCC010000276 DAOSCC010000000
VERSION     DAOSCC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25306_length_2636_cov_3.777606"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="rsmB"
                     /locus_tag="PLG72_10995"
     CDS             <1..1250
                     /gene="rsmB"
                     /locus_tag="PLG72_10995"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOJ81362.1"
                     /translation="EISERHRAFITELVYGVVKRKLTLDRTAAACSNIKMERLSPWIK
                     NILRLGTYQLLYLTKVPPSAAVNESVKLAGRYGHKASAGYVNAVLRNIAGGGGKDIVP
                     DRAVDPVGYLSVRYSYPKWIADKFMELFGEEFAESLLEAGNRVPDLTVRANTLKTSAE
                     GLVNALKNEGVEASPGRYVSDAVIIKSQVSVAGLEAFRNGLFQVQDESSMLPAAVLAP
                     QPGEKVLDACSAPGGKATHMAQMMKNRGLITAMDIHGHKLKLIEDAAGRLGIDIISTE
                     LHDASVPVPRHEGAYDRALLDAPCSGLGIIRRKPDIKWARESRDIGSITALQRRLIDS
                     VSKAVRPGGVMVYSTCTLLPEENERIVRDFLNNNDEFYEDDIAAFLPHELSKHARGGM
                     LQVYPNRDGIDGFFIARLARKEK"
     gene            1324..2427
                     /gene="rlmN"
                     /locus_tag="PLG72_11000"
     CDS             1324..2427
                     /gene="rlmN"
                     /locus_tag="PLG72_11000"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOJ81363.1"
                     /translation="MYLPDLNICELEEFFTGIGQKKYRAKQVFSWIARGAGSFDEMTD
                     LPLSLREELKLLASVSGLKVRKKLVSAIDGTVKYLFGLDDGNVIESVLMEYIHGYTAC
                     VSSQVGCKMGCSFCASTIAGFRRNLTAGEILGQILAIQRDSGVKVNNVVVMGIGEPFD
                     NYDNVVKFLRLANSHDGLNIGYRHMTVSTCGLVPGMLRFADEGFQVNLAISLHASNDR
                     IREKLMPVGRKYRIDKIIEASKIYTEKTNRRIMFEYALIEGVNDSLENAAELAFLLKG
                     LLCLVNLIPVNPVEGISYRHSGRRRIEEFAAILKEKGIETTIRRELGSDINAACGQLR
                     RSEINAEKGAEQANEAKDSASAESNAVSKSECK"
BASE COUNT          719 a          568 c          812 g          537 t
ORIGIN      
        1 atgagatttc cgaaaggcac agggcgttca taaccgaact cgtctacggc gtcgttaagc
       61 ggaaactcac gctggacagg acggcggccg catgttccaa tataaagatg gagaggctgt
      121 ctccgtggat aaaaaatatc ctgcgccttg ggacgtatca actgctgtat ctgacaaaag
      181 tgcccccttc cgcggcagtg aatgagagtg tcaaacttgc gggcagatac gggcataagg
      241 cctcggccgg gtatgtcaac gctgtgctga ggaacattgc gggaggcggc gggaaagata
      301 ttgtcccgga cagggcagtg gacccggtag ggtatctttc ggtcaggtat tcgtatccca
      361 aatggatcgc agataaattc atggaactgt tcggggaaga atttgcagag agtctgctgg
      421 aagctggcaa cagggttcca gaccttacag tgagggccaa cacgctgaag accagcgccg
      481 aaggattggt caatgcgctg aaaaacgaag gcgtcgaagc gtcaccggga agatatgtca
      541 gcgatgcggt gataataaaa agccaggttt cggttgccgg gctcgaggca ttcagaaacg
      601 gactgttcca ggtgcaggat gagagttcca tgctgcctgc ggcagtgctg gcgccccagc
      661 cgggcgaaaa ggtgctcgac gcctgcagcg cgcccggagg gaaagctacg catatggccc
      721 agatgatgaa aaacaggggt ctcattacag cgatggacat acacgggcat aagcttaaac
      781 tgatagaaga tgcggccggc aggctcggaa tcgatataat cagtacggaa ctgcatgatg
      841 cgtcggtccc cgtaccgcgg cacgagggag cgtatgacag ggcgctcctg gacgctccgt
      901 gttcaggcct cgggataata aggaggaagc ctgatatcaa gtgggcaagg gagagcaggg
      961 atatcgggag catcacggca ctgcagcgac ggctgataga cagtgtttca aaagctgtga
     1021 gacccggcgg cgtgatggtc tacagcacat gcacgctgct gccggaagaa aacgaacgca
     1081 tcgtaaggga ttttctgaat aataatgatg agttttatga agacgatatc gccgcatttc
     1141 tgccgcatga gctttcgaag cacgccaggg gcggcatgct gcaggtttat cccaacaggg
     1201 acgggataga cggtttcttt atcgcaagac tggcaaggaa ggagaaatag cggattggca
     1261 agcgatttgg gaaaggtatg cccgggcggt tcaggaaata taaatgcgga cggttcgggg
     1321 aaaatctatc ttccggattt gaatatatgc gaactcgagg agttttttac gggcatcggg
     1381 caaaagaaat accgggcaaa gcaggtattc agctggatag cgaggggagc cgggagtttt
     1441 gacgagatga cggaccttcc gctgtcgctg agggaagagc tgaaactttt agccagtgtt
     1501 tccggtctaa aagtaagaaa aaagctcgta tctgccatcg acggtactgt aaagtatctg
     1561 ttcggactgg atgacggcaa tgtcatcgaa agcgtgctga tggagtatat acatggctac
     1621 acggcatgtg tttcttcaca ggtaggctgc aaaatgggct gcagtttctg cgcgtcgacg
     1681 atagccgggt tcaggaggaa tctcactgcc ggcgagatac tcggacagat actggcgata
     1741 cagcgggaca gcggcgtaaa ggtgaacaat gtggtcgtta tggggatagg cgagccgttc
     1801 gacaactacg acaatgtcgt caaattcctg aggctggcga attcccatga tggattgaat
     1861 ataggataca ggcacatgac tgtttcgacg tgcgggctcg tgccgggcat gctcaggttt
     1921 gccgacgaag gcttccaggt gaatcttgcg atttcgctgc atgcatccaa tgaccgtata
     1981 agggagaagc tgatgccggt ggggaggaag tatcgtattg acaaaataat tgaagctagt
     2041 aagatatata cagagaaaac caaccgcagg atcatgttcg aatatgcctt gatagaaggc
     2101 gtcaacgatt cgctggaaaa cgctgctgag cttgccttcc tgttaaaagg acttttgtgc
     2161 cttgtaaacc ttataccggt aaatccagtg gaggggatca gttaccgcca tagcggccgc
     2221 aggcgcattg aggaatttgc tgcgatcctg aaagagaagg gtatcgaaac gacgatccgc
     2281 cgcgaacttg gctcagatat aaacgcggca tgcggacagc tgaggcggag cgaaataaac
     2341 gccgaaaaag gggcagagca ggcaaatgag gcaaaagact cggcaagtgc agagtcaaat
     2401 gcagtaagta aaagtgaatg taagtaaatc aggtaatgaa gagcagtgta ataccggtcg
     2461 aaatgcaaaa caggacgaca ttattctaag gcaggtgctg atggcttgaa atatgcagca
     2521 ggtaccgaca gaggattatt gagggacata aatgaagaca gctatatgat catcccgggg
     2581 cactcgggca cgtcctgcgc cttcgacggt ccatgggccg ccatggcctg cctgca
//
LOCUS       DAOSCC010000277         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_25307_length_2636_cov_3.677644, whole genome shotgun sequence.
ACCESSION   DAOSCC010000277 DAOSCC010000000
VERSION     DAOSCC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25307_length_2636_cov_3.677644"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..985
                     /gene="metA"
                     /locus_tag="PLG72_11005"
     CDS             50..985
                     /gene="metA"
                     /locus_tag="PLG72_11005"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOJ81364.1"
                     /translation="MPIRIPDSLPAAEVLNSENIFVMTERRAESQDIRPLNIVVLNLM
                     PTKIVTETQLLRLIGNTPLQVDVTFLHPKSHTSKNTPQEHLLKFYNTFDEIRDRRFDG
                     LIITGAPVEQLPFEEVDYWEELKEIMEWSLHNVYSTFHICWGAQAGLYYHYGIPKYPL
                     EKKLSGVYKHRIVKKNVMLLRGFDDEFYVPHSRYTEVRREDIEKSGELEILAESDEAG
                     VYIVLALNGRQIFVTGHSEYDQLTLKYEYDRDMGRGLDPQIPENYFPNDDPERMPVVG
                     WRAHANLLFANWLNYYVYQETPFDLNQLDPARKAK"
     gene            complement(1184..2497)
                     /locus_tag="PLG72_11010"
     CDS             complement(1184..2497)
                     /locus_tag="PLG72_11010"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HOJ81365.1"
                     /translation="MDERIKSFLDALAANLEGLAEDEKREALDYYEEYFSDALEEGAD
                     PGELLSRLDPPEKIAAAIRAETSIRLVNADPGLKNYSRLVKSAHAGITGPLSVFLLSL
                     VIFTTYSIAIILFLCTVVSAAAACIILPASISEAFKIPARYIGEIAGTIGIGIFASGV
                     CLLIAYGFYVLCRPLIRVSASLVYKMVKKGSKPGSNTVEAHQDVSGVTGPKRVCRSLR
                     AGLIITAAGLAVSLASGLPVKLFMIFNSMKPADITVIQREFGMDGISEISIETAHSNI
                     RLTEGGSGNISLEYEKSNWLDFDIKSDGGRVVFTERSNGRLPLFSLVSLHENRAELII
                     SLPSGWEPGSVRLESRGGTINIESGSFPVQAKTYTGTIHLTVPADGDLQEIPPAIRAK
                     SSKGAIFLQDKTTGAKRPYGTEYELQSPGGVTIRLESERGNIVLD"
BASE COUNT          646 a          642 c          693 g          655 t
ORIGIN      
        1 agtgttaacc gggcgggagc cgaacgagta catgaaaggt ggaaaatgaa tgcctataag
       61 gattccggac agcctgcctg ctgcagaggt gctgaatagc gaaaatatat tcgtgatgac
      121 agagcggagg gctgaaagcc aggatatcag gcccctgaat atcgtcgttc tcaacctgat
      181 gcctacaaag atcgtaactg agacgcagct gctgaggctt ataggaaaca cgccgctcca
      241 ggtggacgtg acatttttgc atcctaagtc acatacgtcg aaaaacaccc cgcaggagca
      301 cctgctgaag ttttacaaca cgttcgacga aatcagggac aggagattcg acggccttat
      361 cataacaggt gcgcctgtcg agcagctgcc gttcgaagag gtggattact gggaagaact
      421 gaaagagata atggagtgga gcctgcacaa cgtttattcc actttccata tctgctgggg
      481 agcccaggcc gggttgtatt accattacgg catcccgaaa tatccgctgg agaaaaaact
      541 gtcaggcgtg tacaaacaca ggatcgtaaa gaagaatgtg atgctgctcc gggggttcga
      601 tgacgaattt tatgtgcctc attcgcgcta cactgaggtc aggagggaag atatcgaaaa
      661 gtccggtgaa ctcgaaatac tggctgagtc cgacgaagcg ggggtttata tagttcttgc
      721 cctgaacgga aggcagatat ttgttaccgg gcattcggag tatgatcaac tgacgcttaa
      781 atacgagtat gacagggaca tgggcagagg actcgacccg cagataccgg aaaattactt
      841 cccgaatgac gatccggaaa ggatgcctgt cgttgggtgg agggcgcatg cgaacctgct
      901 gtttgccaac tggctcaact attacgtata ccaggagact cctttcgatc tgaatcaact
      961 ggatccagcc aggaaggcaa agtaacgggg aaactgtcag atatacggaa ggctggtcgg
     1021 agaccagcct tttattgtac atacctgacc ggtatatcgg cgttcggatc gaactcaaac
     1081 tctttggttg aaaggttgcc gacattctgt gaatgatagt gcaataaatt gggatcgaag
     1141 caaaaagaaa gtgcaaccat tccccaaaac aggtctttag ggatcaatcc agaactatat
     1201 tccctctttc agattcgagc ctgatggtaa cgccgcctgg cgattgcagc tcatattcag
     1261 taccataagg ccttttggcg ccggtggttt tatcctgtaa gaatatggcg ccttttgatg
     1321 atttggcccg gatggccggt ggtatctcct gcaggtctcc gtctgcagga acagtcaggt
     1381 gtatggtgcc tgtataagtc tttgcctgga ccgggaagct gccgctttcg atatttatgg
     1441 ttccgcccct gctttccagg cgtacgctgc ccggttccca gccagacgga agtgaaatga
     1501 tcagctcggc gcggttttca tgcagtgaaa ccagcgagaa caggggaagc ctgccgttgg
     1561 atctctccgt gaacactacc ctgcctccgt cgctttttat atcgaaatcg agccagttgg
     1621 atttttcata ctcgaggctt atatttccag agccgccttc cgtcagcctt atatttgaat
     1681 gtgccgtttc tatgcttatt tccgatattc cgtccatgcc gaactcccgc tgtataacag
     1741 tgatatctgc gggtttcatg ctgttgaata tcataaacag cttgacggga agcccggatg
     1801 ccagggagac agcaagcccg gcagctgtta tgatgagacc tgctctcagt gaccggcaga
     1861 cccgtttcgg cccggtaacg ccggatacgt cctggtgcgc ttcaaccgta ttgctgcctg
     1921 gctttgagcc ttttttcacc attttgtaaa ccaggctggc agacaccctg atcagtggcc
     1981 ggcacagtac gtaaaacccg tatgcgatca gcaaacagac gcctgacgca aatatcccga
     2041 tcccgatcgt gcctgcgatc tcaccgatgt accgtgcagg tattttaaaa gcttcagata
     2101 tcgatgcagg caggatgatg catgcggcgg cggccgaaac aacagtacag aggaacagta
     2161 ttattgcgat gctgtatgtc gtgaagataa cgagcgaaag caggaatacc gacaatggcc
     2221 ctgtgatccc ggcatgtgcc gatttgacca gccttgaata gtttttcagg cctggatcgg
     2281 catttacgag cctgatgctg gtttcggccc tgatggcagc agcgattttt tcgggaggat
     2341 cgagccttga aagcagttcg ccggggtccg ctccctcttc caatgcatcg ctgaaatatt
     2401 cttcatagta atccagggct tctctttttt cgtcttcggc aaggccttcc aggtttgctg
     2461 ccagggcatc gagaaagctt tttatccttt catccatttg catcaccgcc tgtaataaaa
     2521 tcaatgatcc ttttcagttc agccagtccg tttttatatt ctgccaagcg ttttgcccct
     2581 gatgcggtta tcctgtaata tcgcctcagg cggcctgaat gctcggcgga ataggt
//
LOCUS       DAOSCC010000278         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_25642_length_2613_cov_3.239249, whole genome shotgun sequence.
ACCESSION   DAOSCC010000278 DAOSCC010000000
VERSION     DAOSCC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25642_length_2613_cov_3.239249"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1147
                     /locus_tag="PLG72_11015"
     CDS             71..1147
                     /locus_tag="PLG72_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81366.1"
                     /translation="MKKILALVMSMALVMFVLAGCGKGATETPGGNGGGVAPGGSDGG
                     GNAAAGDLKTGFAVITSITKSTDAGDNPGLAQVDSTVAAVTVDAQGKIVNAVFDAVQT
                     KINFTAEGKITTPLDTNVRTKQELKTEYGMSKASGIGKDWNEQADAFAEYVKGKTISE
                     VKGIALNDSGAPADAELSSSVTMHVTDLIAVLEKAVNNAQALGAKPGDKLGLGIVTTI
                     SNSADATAGKDGVAQAYSIYTAATFGADGKITSSIIDGSQSNVNFNTSGKITTDLQNA
                     NLQTKNELGDQYGMRKASGIGKEWNEQAAAFAEYVKGKTISEVKGISLNEEGRPAEAD
                     LTSSVTVHVTDFISVLEKASQNAR"
     gene            1346..2515
                     /locus_tag="PLG72_11020"
     CDS             1346..2515
                     /locus_tag="PLG72_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOJ81367.1"
                     /translation="MNQDMTGDKKEMLLKDFMFSVLGREKALFSRKRTGRKTIGELAS
                     LIMSVVLVTAMPSSLAGCGAKKPVRYEATFLRLFDTKTTIIAYMDDKEEFARYSQLIY
                     DNLEEYHKLYDIYNNYEGISNIKTINDMAGKVPVKVDKRIIDLLEYAKEWYEKTDGEM
                     NVAFGAVLRIWHDYRERGMEDPDSAELPPMEDLVEASKHTDINKVIIDEEASTVFLAD
                     PEMSLDVGAIAKGYAVEQVSRIAAENGFTSGLISVGGNVRAIGSKDGEGKPWNVGIQN
                     PDPGEGSNEIHVLDVVDASVVTSGVYERYYTVEGKNYHHIIDPDTLYPSDEFKSVSIV
                     CKDSGMADALSTAVFNMSLDEGMEFIESIPDTEAFWVLSSGELKYSSHFRDYIKK"
BASE COUNT          774 a          549 c          737 g          553 t
ORIGIN      
        1 cgaaaggctg cgtttataac attgcacaga atttgacgga ggaaaacaag tagtattgga
       61 ggggctattt atgaaaaaga ttttagcact tgtcatgagt atggctttag taatgtttgt
      121 acttgccgga tgcggcaagg gcgcaacgga aactcccgga ggcaacggag gcggtgttgc
      181 gcctggcggc agtgacggcg gcggcaatgc tgcggccgga gatcttaaaa caggcttcgc
      241 agtcataaca tcgatcacca aatccacaga tgcaggcgac aatcccggcc tcgcacaggt
      301 cgattcgacg gttgccgctg tgacggtcga cgcccaggga aagatagtca acgcagtttt
      361 cgatgcggta cagacgaaaa tcaatttcac tgccgaaggc aagataacga cgcctcttga
      421 caccaatgtg aggaccaagc aggaacttaa gacggaatac ggcatgagca aggcttcagg
      481 tatcgggaag gattggaatg agcaggccga tgctttcgcg gagtatgtaa agggcaagac
      541 catcagtgaa gtgaaaggga tagcgctgaa cgactccgga gcacctgccg acgccgaact
      601 gtcttcatct gtgacgatgc atgtcacgga cctgatcgca gtgctggaga aagcagtgaa
      661 caatgcacag gctttgggcg caaagccggg tgacaaactg ggcctcggca tcgtgaccac
      721 catcagcaat tctgctgacg ccacggctgg caaggacggc gtcgcccagg catattcgat
      781 ctataccgcg gctacattcg gcgctgacgg taaaatcacc agcagcataa tcgacggttc
      841 ccagtcaaat gtcaatttca atacatcggg gaagatcact accgacctgc agaatgccaa
      901 cctgcagaca aagaacgaac tgggagacca atacggcatg aggaaggcat ccggcatcgg
      961 taaggaatgg aatgaacagg ccgctgcatt tgcggaatat gtaaaaggca agactataag
     1021 cgaggtaaag ggcatatccc tgaatgaaga gggcaggcct gcggaagccg atctgacaag
     1081 ctctgtgaca gtgcatgtga cagacttcat ctcggtactc gaaaaggctt cacagaacgc
     1141 aagatagaaa aatcggcgat tttgtggtaa tattggtcag agaggcatga tttttgcctc
     1201 tctgatttat tttatcgtaa agaaaggata gtaatgagcc ccgggaacta taatacgggt
     1261 cctgtataag acgcatgatg tgatgggttc catatcgggt atgaaagatt tcgcaccagg
     1321 cggaaagtta tattgattga aagtgatgaa ccaggacatg acaggcgata aaaaggaaat
     1381 gttattgaaa gatttcatgt tcagtgtgtt aggtagggaa aaagcattgt tcagcaggaa
     1441 gagaactggc cggaagacaa tcggcgaatt agcttcgctg ataatgagcg ttgttcttgt
     1501 aacagccatg ccttcgagcc tggccggatg cggcgcaaaa aagcctgtac gctatgaggc
     1561 tacttttctc aggctgttcg ataccaagac gaccataata gcctatatgg atgataaaga
     1621 agagtttgca aggtattcgc agctgatata tgacaatctt gaggagtacc acaaactcta
     1681 cgatatctac aacaactatg aagggatcag caatatcaaa acaataaatg acatggcggg
     1741 caaggtgccg gtcaaggtcg acaaaagaat aatcgacctt ttggaatatg cgaaggaatg
     1801 gtatgagaag accgacggcg aaatgaacgt agcttttggt gcggtgctgc ggatctggca
     1861 tgattacagg gaaaggggca tggaagaccc ggattctgcg gaactgccgc cgatggaaga
     1921 tttggtggaa gcttcgaagc atacagatat aaacaaagta ataatagatg aggaagcgtc
     1981 cacggtattc ctggcagacc ctgagatgag cctggatgtc ggggcgatag caaaagggta
     2041 tgccgtagag caggtgagca gaattgctgc agaaaacggt ttcacttcag gactcatcag
     2101 cgtcgggggc aacgtccgcg ccatcggctc caaagatggt gaaggaaagc catggaacgt
     2161 gggaatccag aatccggatc ccggagaagg cagcaatgag atccatgtat tggacgtcgt
     2221 cgatgcttca gtagtcacca gcggcgtata cgaaaggtac tatactgtcg aagggaagaa
     2281 ttaccaccac atcatagacc ctgatacgct ttatccgtca gacgagttca agtccgtatc
     2341 gatagtctgc aaagactcgg ggatggcgga tgcattgtct acggctgttt tcaacatgtc
     2401 tcttgatgaa ggcatggaat tcatcgaatc gatcccggac acggaagcgt tctgggtgct
     2461 gagcagcgga gaactcaaat attcgagcca tttcagggac tatatcaaaa aatagggatt
     2521 tgcgtcggaa gcaaagttat aaaacacatg agcattggga aatgctaata taagtttgta
     2581 cggtttcagc ctgcgccaca ggcgtttgag aca
//
LOCUS       DAOSCC010000279         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_26257_length_2569_cov_3.531026, whole genome shotgun sequence.
ACCESSION   DAOSCC010000279 DAOSCC010000000
VERSION     DAOSCC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26257_length_2569_cov_3.531026"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="PLG72_11025"
     CDS             complement(<1..1930)
                     /locus_tag="PLG72_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOJ81368.1"
                     /translation="MKQGFRLNEESGYFQNLSVDVMAFSDIYPEGHQSGVSIIMHGNR
                     VATNGDIRFEQTPGQWQPVPKQISREMDEAANTITAVLGYPDPSRHLKGLNPMIYPDF
                     KFNYKVTVKGDGPSVIITVDLDRPIPERFVGKICFNLELFPGALFGKPWIMDDKHGIF
                     PKQPNGPVLKMPSNYLHIWNFSEPDAKADAERLADGNKEYSPIVADDIIAEPYAVGRR
                     FIVRPDDPYNRLVIESMGTELKLYDGRMNHNNGWFVVSSEVPAGATEKAIRWVITPNV
                     VEGWMYNPVIQISQVGYHPEQAKTAVIELDRRDTERRKPVLIKITENGEEEVFSAEAQ
                     EWGRFLRYNYLRFDFTQVKEEGLYKIRYGSSESSIFRIAEDVYDRGVWQPVLEYFLPV
                     QMCHMRVSEKYRVWHGLCHNDDARMAPVDYNHFDGYRQGPSTLTRYKPGDVVPGLNAG
                     GWHDAGDFDLRVESQSGEVYILTLAYESFNVYYDVTTIDQERKITEIHQPDGKNDILQ
                     QIEHGVLSIAGAYRSLGRLYRGIISSHLRQYVHLGDSAVMTDGIKGNDDDRWVFTEDN
                     PIRELTTAAHLAAASRALKGFNDKLSDEALEIARELFRITDGSGDAKTAKLHAAAELL
                     LTTGEQEYREFLLSETDFI"
     gene            complement(2089..>2569)
                     /locus_tag="PLG72_11030"
     CDS             complement(2089..>2569)
                     /locus_tag="PLG72_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81369.1"
                     /translation="ILGFLSLVMFHVHFFYVFALAARYRNSVFRHLTNSRIIFSRFIV
                     RSLICTALAAFEIWLFFFYDWLLLFVGVFMAPVLVIGTKSAFVLRLFRIIEAESGLAE
                     TKRDTDSGADDYTYAGGRNTEGNGKGRNGDSGGENRAGRSEESVNKDGNGDENGYLI"
BASE COUNT          576 a          732 c          599 g          662 t
ORIGIN      
        1 ctatgaagtc agtttccgac agcaggaact ccctgtattc ctgttccccg gtagtcagca
       61 gcagttcggc ggctgcatgc agtttagctg tttttgcgtc gccgcttccg tccgtgatcc
      121 tgaaaagttc acgggcgatc tccagcgcct catcgctcag cttgtcgttg aagcccttca
      181 acgcgcggga agcagcagcc agatgggcgg cggtcgtcag ttctcttatt ggattgtctt
      241 ctgtaaacac ccaccggtcg tcgtcattcc ctttgatccc gtccgtcatg accgcactgt
      301 cgccgaggtg cacatactgt ctcagatggc tgctgataat gccgcgatag agccgcccca
      361 gcgaacggta tgcacctgca atggaaagca cgccatgctc tatctgctgg aggatgtcgt
      421 ttttgccgtc aggctggtgg atctcggtaa ttttcctctc ctggtcgatg gtggtcacgt
      481 cgtaatagac attaaaagat tcatatgcca gcgtcaggat atatacctcg ccggactgcg
      541 attccacgcg gagatcaaaa tcgccggcat catgccagcc gcctgcgttc agcccaggaa
      601 ccacatctcc cggcttgtac cgggtcagag tggaaggtcc ctgcctgtat ccgtcaaagt
      661 ggttataatc gacaggggcc atcctggcgt catcattatg gcaaagcccg tgccatacgc
      721 ggtacttctc gctgaccctc atatggcaca tctgcaccgg gaggaagtac tccagcaccg
      781 gctgccagac tccgcggtcg taaacatctt cagctatacg gaaaatggac gactccgatg
      841 agccatagcg tattttatac aaaccttctt cttttacctg cgtgaaatcg aacctaaggt
      901 agttatagcg gaggaacctt ccccattcct gcgcttcagc cgaaaagact tcttcttcac
      961 cattttccgt tattttaata agcacaggtt tccttcgttc cgtatcccgc ctgtcaagct
     1021 ctataaccgc tgttttggcc tgctcgggat gataccccac ctgcgatatc tggatgaccg
     1081 gattgtacat ccagccttct acgacattcg gagttatgac ccaccggata gctttctcag
     1141 tcgcaccggc aggcacctcg ctgcttacca cgaaccagcc gttgttgtgg ttcatacggc
     1201 cgtcgtacag tttcagttca gtacccatgc tctctatcac aaggcggttg tacgggtcgt
     1261 ccggccttac gatgaaacgc ctgccgactg cataaggctc ggcgattatg tcatccgcga
     1321 caattggact atactcttta ttgccatcgg caaggcgttc tgcgtccgcc tttgcgtcag
     1381 gctcgctgaa attccagata tgtaagtaat tcgacggcat cttcagcaca ggaccgttgg
     1441 gctgcttcgg gaagatacca tgcttgtcgt ccattatcca gggttttccg aacaaagctc
     1501 ccggaaacaa ctcaaggttg aagcatattt tgccgacgaa cctctccggt atgggcctgt
     1561 ccaggtccac agtgattatc actgacggtc cgtcgccttt gacggtgacc ttgtaattga
     1621 acttaaaatc cggatagatc atcggattca aacccttcaa atgccttgac gggtccgggt
     1681 agccaagcac agcagtgatc gtgttggctg cttcgtccat ttcacggctg atctgcttcg
     1741 gcaccggctg ccactgcccc ggcgtctgct cgaaacgtat gtccccgttc gtagcgacac
     1801 ggttgccgtg catgatgatg ctcacacccg actgatgccc ttccggatag atatcgctga
     1861 aggccatcac gtcaacgctc aggttctgaa aataaccgga ttcttcgttc agtctgaaac
     1921 cctgtttcat attgctggta cctccattat cactgtttct ggtgttcccg ataactctga
     1981 cattggtgtg cgtccacaag tttatcccat atcaatcccc tgccgcgcac cttgtcattt
     2041 tgcaataagg ctgtcatggc aaatattata aatgatgcct gacatacatt atatcagata
     2101 gccattttcg tctccattgc catctttatt aacactttct tcagatcttc ccgcacggtt
     2161 ttcaccgcca ctgtccccgt ttcttccttt accgttgcct tcggtgtttc ttccgccggc
     2221 gtaagtataa tcatctgctc cactgtctgt atcccgctta gtctccgcca acccgctttc
     2281 agcttctata atgcggaaaa gcctgaggac gaaagcgctt ttggtcccta tgaccagtac
     2341 cggtgccatg aacacgccga cgaaaaggag cagccagtca taaaagaaaa agagccatat
     2401 ttcgaaagcc gcaagggcag tacagatcag cgaacgtaca ataaagcggg aaaaaatgat
     2461 cctggaattc gtcagatggc ggaaaaccga gttccgatac ctggctgcga gagcaaaaac
     2521 atagaagaaa tgcacatgga acatcaccag gctcagaaag cccagtatc
//
LOCUS       DAOSCC010000280         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_26335_length_2563_cov_5.206140, whole genome shotgun sequence.
ACCESSION   DAOSCC010000280 DAOSCC010000000
VERSION     DAOSCC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26335_length_2563_cov_5.206140"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLG72_11035"
     CDS             complement(<1..460)
                     /locus_tag="PLG72_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ81370.1"
                     /translation="MTKTIGVLIIDDSSFIRKVLRDILDGTHDIKVIGAAKNGLEALD
                     MARELHPDVITLDLNMPSMDGLTCLRELQKITDAPVIMLSSLTVDGGKATIEALESGA
                     IDFVTKPANIFDVSGEQKKAEIIRKIRMAYGFSKKKQAFPKKTGYTSDGPE"
     gene            complement(460..1152)
                     /locus_tag="PLG72_11040"
     CDS             complement(460..1152)
                     /locus_tag="PLG72_11040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ81371.1"
                     /translation="MELADIETGTRLELEPENSTYVGKTVLVSSFDTAEGIDTALITA
                     PISEGRIVPLDRDSVYNVCFLKRDGKMLNLYKFSAAIRERLVVDNIHLLLIEKLGEIA
                     RIQRRSFFRLDCYVDVRYRVINPSDNGRNSDIPFRKTITSDLSGGGIRLLLDEKLKPG
                     TYLECELFSETDRKIRFYGKVVRFEYVESEGKYRYAAGIAYVDIDENDREEVIRYIFS
                     EQRKLLKKGWNR"
     gene            complement(1173..2093)
                     /locus_tag="PLG72_11045"
     CDS             complement(1173..2093)
                     /locus_tag="PLG72_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOJ81372.1"
                     /translation="MDQAEKLRQVIDVLKLRNKNNMAAMPAKRSARVITVTSGKGGVG
                     KTNVSINLAIALSELGYRTVIIDADFGLANIDVLLGIVPKYTLLDVVKNRKDITEILT
                     DGPSNIKFISGGSGVEDLLKLDREQIARFIENIALLDRITDFIIVDTGAGLSDNVMSF
                     IMAADELLLVTTPEPTSITDAYALIKMVSNKDRMMSNTLKAKKIRLVVNRAENSEEAN
                     DVLNNLVMVAERFLGLGLEPAGYILSDSAVKRSVKQQQPFILAYPKSQAARSIREISS
                     KLANFPDAEEAQQSLGIRGFFNRLVSVMRT"
     gene            complement(2080..>2563)
                     /locus_tag="PLG72_11050"
     CDS             complement(2080..>2563)
                     /locus_tag="PLG72_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOJ81373.1"
                     /translation="YRIAAVEQLKTYAEILGIPVSVAYTPAEMKEAVSLHSDKDLILI
                     DTAGRSHKNKAQFEELKALIEASEADEIYLVLSATTSSKNCREILKSYSFLKDYKLLF
                     TKTDETPVLGIILNARYITGKSLSYITTGQSVPDDIETANIDSITKNLVGSNLRDGSS
                     "
BASE COUNT          549 a          701 c          510 g          803 t
ORIGIN      
        1 tctcggggcc gtcagacgta tacccggtct tcttcgggaa agcctgtttc tttttgctga
       61 aaccgtaagc catcctgatc ttccggatta tctcagcctt tttctgctct ccggatacat
      121 caaatatgtt ggccggcttt gtaacaaaat caatagcccc tgattccaga gcttctatcg
      181 tagctttccc cccgtcgaca gtcagactgc tgagcattat gacaggagca tcggtaattt
      241 tctgcagttc cctgagacac gtcagcccat ccatcgacgg catgttcagg tcgagcgtta
      301 tgacatcggg atggagctca cgcgccatgt ccagtgcttc cagcccgttt ttggctgccc
      361 cgataacctt tatatcatgt gtgccgtcca gtatatccct cagcactttc cgaataaagg
      421 acgaatcgtc tataataagt actcctatcg ttttcgtcat tacctgttcc agcctttttt
      481 gagcaatttg cgctgttcgc tgaatatata cctgatgact tcttccctgt cattttcatc
      541 tatatcgaca taagctatgc cggcggcata cctgtatttt ccttccgatt ccacgtactc
      601 gaacctgacg actttcccgt aaaaccggat ctttctgtca gtttcgctga aaagctcaca
      661 ttccagatat gtaccgggtt taagcttctc atcaagcaaa aggcgtatcc cgccgccgct
      721 cagatcgctc gtgatggttt ttctgaacgg gatatcactg tttctcccgt tatcggacgg
      781 atttatgact ctgtaccgga catccacata acaatccagc cggaaaaaac ttcgtctctg
      841 tatccttgct atctctccaa gcttctctat caaaagcagg tgtatgttgt cgaccaccag
      901 cctctccctg atggctgcac tgaatttata tagattaagc attttgccgt ctcttttaag
      961 aaagcagaca ttatataccg agtctctgtc cagcggaacg atcctgcctt cagaaatagg
     1021 agccgtaatc aaagccgtat caatgccttc cgccgtgtcg aaactgctca ccaggactgt
     1081 ctttccgacg tatgtgctgt tttccggctc aagttccagc cgtgtacctg tttcaatatc
     1141 agccagttcc atacgcatct cccgggttcc atctatgttc tcataacact gacaagcctg
     1201 ttgaaaaatc ccctgatgcc aaggctctgc tgtgcttctt ctgcatccgg gaagtttgcc
     1261 agcttcgatg atatttctct gatacttctg gccgcctggc tcttggggta agccaggata
     1321 aatggctgct gctgctttac cgaccttttt actgcgctgt cgctcaatat gtaacctgca
     1381 ggttccagtc cgagtcctaa aaacctttca gccaccatca ccagattgtt cagtacatca
     1441 ttggcctcct cgctgttttc agccctgttg acgacaagcc ttatcttttt ggccttgagc
     1501 gtgttggaca tcatcctgtc cttgttggac accatcttta tcagagcgta tgcatccgtt
     1561 atcgatgtag gttcaggtgt cgttaccaat agtaactcat cggcggccat tatgaaactc
     1621 atcacattat ctgacaatcc tgcgcccgta tcaacgataa taaaatccgt tatccggtcg
     1681 agcagcgcta tgttttcaat aaacctggct atttgctccc tgtccagttt gagcagatct
     1741 tccactccgg atccgcccga tatgaacttg atgttgctgg gcccgtcagt cagtatctca
     1801 gtaatgtctt tcctgttttt tacaacatcc agcagcgtat atttcggtac tattcccagc
     1861 aagacatcga tattcgccag gccgaaatcc gcatcgatta tcactgttct gtatcccagt
     1921 tcactcaaag ctatggccag attgatcgaa acgtttgttt tgccgactcc tcctttaccg
     1981 cttgtaacag tgataaccct ggctgatctt tttgccggca ttgccgccat gttgtttttg
     2041 ttccgcagtt tcagaacatc tattacctgt ctgagttttt cagcttgatc catctctcag
     2101 gttactccct accaaattct ttgttatact gtcgatattt gccgtttcaa tgtcatctgg
     2161 cacgctttgt ccggtcgtga tatatgaaag gcttttcccc gttatatatc ttgcattgag
     2221 tatgatgccc agtaccggag tttcgtctgt ctttgtaaat aacagcttgt aatcctttaa
     2281 aaagctgtaa cttttcaata tctccctgca attcttcgaa cttgtggtgg cgctgaggac
     2341 caggtatatc tcgtcggctt cggaagcctc tatcagcgcc ttcagttctt caaactgggc
     2401 tttgttttta tggctcctgc ctgccgtatc tatcaggatc agatccttgt ccgaatgcag
     2461 gctcactgcc tccttcatct ctgccggcgt atatgcgacc gataccggga tccccagtat
     2521 ctccgcataa gtcttcagtt gttccacagc tgcgatcctg tat
//
LOCUS       DAOSCC010000281         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_26643_length_2540_cov_4.065594, whole genome shotgun sequence.
ACCESSION   DAOSCC010000281 DAOSCC010000000
VERSION     DAOSCC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26643_length_2540_cov_4.065594"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLG72_11055"
     CDS             complement(<1..1073)
                     /locus_tag="PLG72_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ81374.1"
                     /translation="MMGKAITKSYKLIIIIIDLILINLSYLTAFFMKFGFNIPMFNLG
                     PYLNAMPFITIAALVYFDMFGMIKFYRVTVSEALSNLVKLVALLSITTVSITYFLQGF
                     SFPRSVLLGSAFIMFIYLSLWRVFLLSIRQKLMWESNAMIIGSQEELKAIGDKITSDK
                     AHKIAIKYEIDVRDADLAFGRLDEVGEVILCSGVPDDFKVRMIKECIERRKVVYAIPQ
                     LLEISLSKTRTIQFEDMPAFIIDRLGLTREQRFFKRAFDIVLSLIGLIISSPVMLISA
                     ILIKATSPGPVIFSQERVTEGNRVFKIYKFRTMVNDAEKTTGPVISSLDDPRVTPIGS
                     VLRKLKIDELPQLFNILKGDMSFV"
     gene            1240..2169
                     /locus_tag="PLG72_11060"
     CDS             1240..2169
                     /locus_tag="PLG72_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ81375.1"
                     /translation="MITILLAACNGEKHIADQIESVLNQTLRNWRLIVQDDCSEDRTC
                     EIVEKYAAMHPDRIVLKKRRIPSGSAKDNYFSMLQYADSDYIMTCDQDDVWLPDKIEV
                     TMHKMLEIEKRIGHDRPILVHTDLKVTDDNLNVLSESLIKYQKLDSSRDKLNNLLAQN
                     IVTGCTMMVNRALADKAQNLPEQAIMHDWWFAMIASAFGQIGFVDKPTVLYRQHAGNA
                     VGAKNVNSIFYKIKRMFTLKQAKMSLAATYAQAESFWNIYGDRLGENEKEIIQAYLQI
                     PGANRMERIRIISRHDFWKTGFFRRCGQIVIRP"
     gene            2234..>2540
                     /locus_tag="PLG72_11065"
     CDS             2234..>2540
                     /locus_tag="PLG72_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ81376.1"
                     /translation="MKGIVLAGGNGTRLYPLTLVTSKQLLPVYDKPMIYYPLSILMLA
                     GIREILIISTPHDLPNFRKLLGDGSDYGIELSYREQPYPGGLAQAFIIGESFIDGESC
                     "
BASE COUNT          739 a          567 c          611 g          623 t
ORIGIN      
        1 acaaagctca tatcaccttt cagtatgttg aaaagctgtg gaagttcgtc aatcttaagt
       61 tttcttaaaa cagagccaat tggcgtcacc ctggggtcgt ccagcgatga aatgacaggg
      121 cctgtagttt tttcggcatc gttcaccatg gtgcggaact tgtagatctt aaacacacgg
      181 ttaccctcgg ttacccgctc ctggctgaat atgaccggac cgggcgatgt agccttaatc
      241 aatatggcac ttatcagcat gacaggcgaa ctgatgatta gccctataag cgacagaaca
      301 atatcaaagg ctcgcttaaa gaagcgctgt tcacgggtaa gcccaagcct gtctatgatg
      361 aaggcgggca tatcctcaaa ttgtatggtt ctggtcttgg acagggatat ctccaaaagt
      421 tgagggatag catacacaac ctttctgcgc tcgatgcact ccttgatcat cctgaccttg
      481 aaatcgtccg gaacaccgga acacaggata acctcgccca cttcatcaag cctgccgaac
      541 gcaaggtcgg catccctcac atctatctcg tatttgatgg ctatcttgtg ggctttatcc
      601 gaggtgatct tgtcgccaat agctttaagc tcttcctggc ttccaattat catggcattg
      661 gattcccaca tcagtttttg ccttatgctc agtaaaaaca ccctccaaag cgaaagatat
      721 atgaacatga taaaggcgga acctaacaga acgcttcgag ggaacgagaa gccctgcagg
      781 aaatatgtaa tggacacggt cgtgatgcta agaagcgcaa ccagcttgac gaggttcgac
      841 aaggcttcgc tcacggttac cctgtaaaac ttgatcatcc cgaacatgtc gaaatagacc
      901 agggcagcta tagtgatgaa tggcatcgcg ttcaggtatg gccctaagtt gaacatgggg
      961 atattaaagc cgaacttcat gaagaaagcg gtaaggtatg aaaggttgat cagtataaga
     1021 tcaataatga tgattattag cttgtatgac tttgtaatgg ccttgcccat cattcacacc
     1081 tttcagagaa gcctgtcgtc ccatgtcttt acccaaatta tatcatggaa gccggtttta
     1141 aaatagtata caataattgt tggaataaca aacaggtggc aagctttgat ataagcacgg
     1201 gaatgacgtc gattctccgg caggtaaggc ggttggcata tgattacgat tttgcttgcg
     1261 gcatgtaatg gagaaaagca tatagccgat caaatcgagt ctgttttaaa ccagaccctc
     1321 cgaaactggc ggcttatcgt tcaggacgac tgctcggaag acagaacatg tgaaatcgtc
     1381 gaaaaatatg cggccatgca tcccgaccgg atagtattga aaaaacggcg aataccttca
     1441 ggttcggcaa aggataatta tttcagtatg ctgcagtatg cggattccga ctatataatg
     1501 acctgcgacc aggatgatgt ctggcttcct gataaaatag aagttacaat gcataaaatg
     1561 ctcgagattg agaagagaat cgggcatgac agaccgatac tggtgcatac cgatctgaaa
     1621 gtaaccgatg ataatctcaa cgtcctgtca gaatcactca taaagtatca aaaacttgac
     1681 agcagcaggg ataagctgaa taacctgctg gctcaaaata tagtaaccgg gtgtaccatg
     1741 atggttaacc gcgcattggc agataaggcg caaaatcttc ccgaacaggc gattatgcat
     1801 gattggtggt ttgcgatgat tgcttcggct ttcgggcaga tcgggtttgt tgacaagccc
     1861 actgtcttgt acaggcagca cgccggcaac gcggtcggag caaagaacgt gaacagtata
     1921 ttctacaaaa taaagaggat gtttacgctg aaacaggcca aaatgtccct ggcagccact
     1981 tatgcccagg cagaaagctt ctggaatatc tatggagacc ggcttggcga aaatgaaaaa
     2041 gaaataatcc aggcatacct gcagataccc ggagcgaaca ggatggaaag aataagaatc
     2101 atatcgaggc atgatttctg gaaaaccgga ttcttccggc gttgcgggca gattgtaatc
     2161 cggccgtaat tgtaatggct tgcccaatat gctataatat ccgagaaatt gtctaccggg
     2221 gaggtaatac ataatgaaag gcatcgttct ggcaggcggc aacggaacaa ggctttatcc
     2281 cctgacgctg gtcacttcga agcaactgct gccagtatat gacaagccta tgatctatta
     2341 tcccctgtca atactcatgc tggccggcat acgcgaaatt cttattattt ctacccccca
     2401 cgacctgcct aatttcagga agctccttgg agacggatcg gactatggaa tagagctgtc
     2461 atacagggag cagccttatc cgggaggact ggcgcaggcc tttatcatag gcgagagttt
     2521 tatcgacggc gaatcatgcg
//
LOCUS       DAOSCC010000282         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_26722_length_2535_cov_3.558065, whole genome shotgun sequence.
ACCESSION   DAOSCC010000282 DAOSCC010000000
VERSION     DAOSCC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26722_length_2535_cov_3.558065"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLG72_11070"
     CDS             <1..463
                     /locus_tag="PLG72_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ81377.1"
                     /translation="ETLRIGGNFHDIGKIGTPDDILLKTDKLSDEELKEIQKHPLKGA
                     RILSAVSMFKDVVPVVKCHHERIDGTGYPEGLSGDSIPLLARIISVADAFDAMLSDRA
                     YRSRLTLEEARQQLLNGSGTQFDSHIVSVFLDIIDKMKDTGLLHETETGRE"
     gene            690..2528
                     /gene="typA"
                     /locus_tag="PLG72_11075"
     CDS             690..2528
                     /gene="typA"
                     /locus_tag="PLG72_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ81378.1"
                     /translation="MTGKRNNIRNIAIIAHVDHGKTTLVDGMLRQSGIFRENEQVQER
                     VMDSNDLERERGITILSKNTSVRYKDIKINIVDTPGHADFGGEVERVLKMVDGVLLLV
                     DAFEGPMPQTRFVLRKALQMNLKPIVVVNKIDRPEARPREVVDEVLDLFIELGADDDQ
                     LEFPVVYASSREGYAVLDINDERRDLEPLFKTIIERVPAPAGEEDKPLQFLVSNIDYD
                     DYVGRIAVGRVDRGRISAGQQVAVCKKDGTVYRSRTGRIYVFEGLKRIECEEAGVGEI
                     VAVSGIDDINIGETICDAEVPEPLPFVDIDEPTVSMTFSVNDSPFAGREGTYVTSRHL
                     RDRLFKELETNVSLRVEETDSADSFKVSGRGELHLSILIETMRRQGYEFQVSKPKVIL
                     KEIDGVVCEPVELLMIDVPEEYMGTVMEKLGSRKAEMTDMRSAAEGYVRLEFKIPARG
                     LIGYRSEFLTDTKGNGIMNHIFYGYEPYKGEITQRQRGSLVAWEDGEAVTYGLYNAQE
                     RGTLFITPGTMVYEGMIVGENSRNEDIVVNVCKKKHVTNMRAAGSDEALRLTSPRILS
                     LEQALEFISDDELVEVTPKNIRLRKRILDTEQRAKARSRLGSGHDK"
BASE COUNT          723 a          547 c          766 g          499 t
ORIGIN      
        1 tgagacgctg aggataggcg gtaatttcca cgatatcggg aaaataggga cgccagacga
       61 tatactgctc aaaacagaca agctgagcga cgaagaactg aaggaaatac agaagcatcc
      121 tctgaaaggc gcaaggatcc tttctgccgt ttcaatgttc aaggatgtgg tacccgtcgt
      181 gaaatgccat catgaacgga tcgacggcac agggtatcct gaaggactca gcggcgattc
      241 cataccattg ctggcaagga taatctctgt agcagacgct tttgatgcga tgctgtccga
      301 cagggcttac agatccaggc tgacccttga ggaagcaaga cagcagctgc tgaacggttc
      361 gggcacacag tttgattccc atatagtctc tgttttcctg gatatcatag ataaaatgaa
      421 agacacaggc ctgctgcatg aaacggagac cggccgtgaa taacagcagc gttgccggag
      481 aggtttcccg gcaagccgat gggaaaaccg ttctgcgctc ccggcggtgt atccgcccgg
      541 gagttttgat tttattgaca ttgatgtttc gatattttaa aatttatctt gctgcatccg
      601 ggagatgtca taaactggat ggatgcggca aatcaaggca aactgcgggc aataaacctg
      661 cttcatgtgg actgaaagga aaggataatg tgacaggaaa gaggaacaat ataagaaata
      721 tagcaataat cgcacatgtg gatcacggaa agacgaccct ggtcgacggg atgctccgcc
      781 aaagcggcat attcagggaa aacgagcagg tacaggaacg ggtgatggac tcaaacgacc
      841 tggaaaggga acgcgggatc accatactgt caaagaatac atcagtcaga tataaggata
      901 taaaaataaa tattgtggat accccgggac atgcggactt cggcggagaa gtggaacgtg
      961 tgctgaaaat ggtggacggc gtcctgctgc ttgtggatgc tttcgaaggg ccgatgcccc
     1021 agacgcgttt tgtcctgaga aaagcacttc aaatgaacct gaagcccatc gttgtcgtca
     1081 ataagataga cagacctgag gcaagacccc gggaagtagt ggatgaagtg ctggatctgt
     1141 tcatagaact gggcgccgac gatgaccagc ttgaatttcc cgttgtgtac gcttcatcca
     1201 gggaaggcta tgccgtgctt gacatcaatg acgagaggcg ggacctggag ccgctgttca
     1261 aaaccataat agaacgtgta ccggccccgg cgggcgagga ggacaaaccg ctgcagtttc
     1321 tggtctcaaa catagattac gacgactacg tgggcaggat agccgtcggc agggtcgaca
     1381 ggggcaggat cagcgccggg cagcaggtgg ccgtgtgcaa aaaggacgga acggtgtacc
     1441 ggtccagaac cggcaggata tatgtgtttg aaggactgaa acgcatcgag tgtgaagagg
     1501 ccggcgtcgg ggaaatagtt gcagtttccg gtatagacga tatcaacata ggcgaaacga
     1561 tctgcgatgc ggaagtgcct gagccgctgc cttttgtaga catagacgag cccaccgtca
     1621 gcatgacgtt cagcgtgaat gacagcccgt ttgccggaag ggaagggacg tatgtcacgt
     1681 ccaggcatct gagggaccgc cttttcaagg aacttgaaac caatgtgagc ctccgggtcg
     1741 aggagactga cagcgcggat tcgttcaaag tttcaggcag gggcgaactc catctctcga
     1801 tactgatcga gacaatgaga aggcagggct atgaattcca ggtatcgaaa ccgaaggtta
     1861 tcctgaagga gatcgacgga gttgtatgtg agcccgtcga attgctgatg atagatgtgc
     1921 cggaagaata tatgggcacg gtgatggaga aactgggcag ccgcaaggcg gaaatgacag
     1981 acatgcgctc cgctgcggaa ggatacgtca ggcttgagtt caagataccc gcaagaggac
     2041 tgatcggcta caggtcggaa tttttgaccg atacgaaggg caatggtata atgaatcaca
     2101 tattctacgg ttatgaaccg tacaagggag agataacgca gagacagaga ggttcgctgg
     2161 tggcatggga agacggggag gcagtgacgt acggcctgta caacgcacag gaaagaggca
     2221 ctctcttcat aacgccgggg accatggtat atgagggaat gatcgtcggc gagaattccc
     2281 ggaacgaaga catagtggtg aacgtctgca agaaaaagca tgtcaccaac atgagggcgg
     2341 caggatctga cgaggcgctg aggcttacat ccccgaggat cctgagcctt gagcaggcgc
     2401 tggaattcat atctgatgac gaactggtgg aagtgactcc gaaaaatata aggctgagaa
     2461 agaggatact ggatacggag cagagggcca aggcaaggag caggcttgga tccggccatg
     2521 acaaataagg ccgaa
//
LOCUS       DAOSCC010000283         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_27058_length_2512_cov_3.466829, whole genome shotgun sequence.
ACCESSION   DAOSCC010000283 DAOSCC010000000
VERSION     DAOSCC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27058_length_2512_cov_3.466829"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLG72_11080"
     CDS             complement(<1..348)
                     /locus_tag="PLG72_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ81379.1"
                     /translation="MAGKKETDSRSTENRIKLFDSELKLMELLWANEGATAKELALLA
                     ADQIGWNKNTTYTVIKKLVAKGAIKRTEPNFVCHSLITREEVGRDEARKVLRSFFGGS
                     VKMLFSSFMADGTL"
     gene            complement(766..1521)
                     /locus_tag="PLG72_11085"
     CDS             complement(766..1521)
                     /locus_tag="PLG72_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ81380.1"
                     /translation="MEYTINRLAKMAGVSTRTLRYYDEIGLLSPARISSNGYRIYGRK
                     EIDRLQQILFYRELGVPLEEIRKIISSKDFNELAALESHLSALLARRKQLDMLIANVE
                     KTIKAAKGEMIMSDREKFEGFKKRLIDENEQKYGSEIRAKYGDEAVDRSNAKLMGMSM
                     EQYEEYEKLTVQFHETLKAAFEQGDPAGELAQKACELHKKWLCFFWDKYSKEAHIGVT
                     RMYVSDPRFTAYFDKIAEGCAAFLRDAVQIYCR"
     gene            complement(1927..>2512)
                     /locus_tag="PLG72_11090"
     CDS             complement(1927..>2512)
                     /locus_tag="PLG72_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HOJ81381.1"
                     /translation="LIFVGEENMAADRRVKYTKMVLRESLIKLLQKKPISRITVKELC
                     ELADINRATFYTHYTDQFDLLRKVSDELIADIASYVDSGLKEGETGLMPMLTMIFEYI
                     DKNSELCRVLLGMNVDIDFQTYVMQIIKDRIVLEWRKKKSVDQSTVDYIYAYVATGCI
                     GVIRKWLFDSDPRTPREMAILITRLANQAVAEFI"
BASE COUNT          538 a          679 c          540 g          755 t
ORIGIN      
        1 cagcgttccg tctgccatga aggacgaaaa cagcattttg accgaccctc cgaaaaacga
       61 gcggagcact ttccttgctt catcacgccc tacttcttcg cgggttatca ggctgtggca
      121 tacgaaattt ggttcagtac gttttatagc cccttttgcc acaagttttt tgataacggt
      181 gtacgtggtg ttcttattcc atcctatctg gtctgccgcc agcaatgcca gttcttttgc
      241 agtcgccccc tcgtttgccc acagcagttc catcagtttc agttcgctgt caaacagctt
      301 gatcctgttt tctgtgcttc tactgtccgt ctctttcttc ccggccatct ttttccctcc
      361 gatcatttga ccgtcgtctt tcatacggtc atcttctcca aaaactttgc tccaacctgc
      421 aagaaggcct ctttcggatc cgatcaggat atttgatcgc agaaatcttc cagcataaat
      481 atatcctgca tcacttggga ccatcatatt taaattacac tgtcccgggc aatctcggac
      541 tattgcaata gtacaatacc agactatcac aatagtctgg tattgtcaat aacagcttca
      601 atttttgatt tgcttattaa aggtttacat gtgcggatcc atttaaggcg atagtgtttg
      661 ccgtacgaag cccgccggat ggcatctgcc tatgatcttt gctaaatcca cttgcgtgat
      721 gacgtctgcc gtctgcatat ggattcctgt ttccctgcgg cagcatcacc tgcagtagat
      781 ctgcaccgcg tccctcagga acgctgcgca tccttcggca attttgtcga agtacgcggt
      841 aaagcgcgga tcagacacat acatccgggt cacgccgata tgtgcttctt tgctgtattt
      901 atcccagaag aagcaaagcc atttcttatg cagttcgcat gccttctgtg caagctcgcc
      961 ggctggatct ccctgttcaa acgccgcttt cagcgtttcg tggaactgga ccgtcagttt
     1021 ctcatattct tcgtactgct ccatgctcat tcccataagc tttgcattcg aacggtcaac
     1081 agcttcgtcc ccgtatttcg cacgtatctc gctgccatac ttttgctcgt tttcatcgat
     1141 aagcctcttt ttgaatcctt cgaatttttc cctgtcactc atgatcatct ctcctttcgc
     1201 cgcctttatg gttttctcca cattggcgat cagcatatcc agttgtttcc ttcttgcgag
     1261 cagggcggac aaatggcttt ccagtgccgc gagttcattg aaatccttcg atgaaattat
     1321 cttcctgatc tcttcaagcg gcacgccaag ttcacggtag aagaggatct gctgcagcct
     1381 gtcgatctcc ttccgtccgt aaatgcggta tccgttcgaa ctgatcctgg ccggcgaaag
     1441 caggccgatc tcatcatagt acctgagcgt acgggtactg acaccggcca tctttgccag
     1501 cctgtttatc gtgtattcca tttgaccacc ctctccggga gataatcata gactattacg
     1561 taacgttaat gtcaatacct gttttagcgt ttgtttttga tcaccgttcc atgttttcag
     1621 aaatggcagt actatcgctg tgaatgtcgg aatgtccttc gtgttttcct ggatcggagc
     1681 ccttatttgc ctgtggatag atgaacggga atcttgctcc gcatgaaccg cattcgatat
     1741 actggtcggc ctcgacctgt tcacgcgtgt catagagaaa ggggcgggca cagcctttta
     1801 acatcctgca tgatatacct ctccgggtcg ggttcgaaat gcaaatccgg tttagccggt
     1861 tcaacctgta gtactatccg gagggcagcg ttttacatgc ggcgatatgg ccggactgac
     1921 cgcggatcag ataaactccg ccactgcctg gtttgcaagc cttgtgatca ggatggccat
     1981 ttccctgggc gtgcggggat cgctgtcgaa cagccactta cgtatcaccc ctatgcagcc
     2041 cgtggccaca tatgcgtaaa tatagtcaac ggtggactgg tcgacagatt tcttttttct
     2101 ccattccagc actatacggt cctttattat ctgcatgaca tatgtctgaa aatcaatgtc
     2161 gacgttcatg ccaagcaaaa cacggcagag ctcgctgttt ttgtcgatat actcaaagat
     2221 catggtaagc atcggcataa ggccggtttc gccctctttc agcccgctgt ccacatagga
     2281 ggcgatatct gcaataagtt catccgagac ctttctcaaa agatcgaact ggtcggtata
     2341 gtgggtatag aacgtcgcac ggttgatatc tgccagttcg cacagttcct ttaccgtgat
     2401 cctggatatg ggttttttct gaagcagctt tatcaggctt tccctcagaa ccatctttgt
     2461 gtatttcaca cgccggtccg cagccatatt ttcctccccg acaaatatca at
//
LOCUS       DAOSCC010000284         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_27305_length_2496_cov_3.659156, whole genome shotgun sequence.
ACCESSION   DAOSCC010000284 DAOSCC010000000
VERSION     DAOSCC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27305_length_2496_cov_3.659156"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..958
                     /locus_tag="PLG72_11095"
     CDS             143..958
                     /locus_tag="PLG72_11095"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOJ81382.1"
                     /translation="MGTGNLIIIGGAEDKTGECLILNSFVEISGASKANIVIITTATE
                     KPIEAGSQYKSLFIGLGAGNVSFLNINTREEAESDQNAMLIAEATGIFFTGGDQLRIT
                     SILGGTKIYETLHQAWKSGAVIAGTSAGASAMSRTMIVDGNDNDPARKCTLKMAPGLG
                     LLDRALIDQHFHQRGRIGRLLCGVAENPSILGIGIDEDTAVRVFPDDHFEVIGSNSVM
                     VVDGKSIMSSNVSELMPDENLAIANVVLHVLPAGYGYDMVNRKVLRVEEKQWK"
     gene            949..>2496
                     /locus_tag="PLG72_11100"
     CDS             949..>2496
                     /locus_tag="PLG72_11100"
                     /inference="COORDINATES: protein motif:HMM:NF040140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOJ81383.1"
                     /translation="MEIIKIEPFKGRNIYSHRPVIRAVADLGGHYDTPTRQIEGFNER
                     LLEMLPGLAKHVCSLGYEGGFAERLAEGTYLAHVAEHMVLELQGMLGFDVHYGKSRIA
                     EEPSIYHIIFEYKNEKTAIECLLAVSDMINVLIAGKMPDIVSIKKHLERVAAESGMGP
                     STEALYEEARKRNIPVVCLEPSSILRLGAGKYTRYLEASLTDSCSCVAVDMAGNKHLT
                     KMKLSEAGIPVPRGDVAYSFRSAAAIAAAIGWPVAVKPFDANQGKGVSTNITSFEELE
                     NAFNAAARFSHAVIVERHIEGKDYRLLVVGGKMIAAAERRAPFVVGDGIHTIRELVEI
                     ENLNPLRGTDHEKPLTIIRIDEMSRQVLAKNGYTENSIPGAGVKVLLRYNGNLSTGGT
                     ARDCTDEVHPHNAELAVKAAELMELDIAGIDITCADISKPLETAGGAVIEVNAAPGLR
                     MHIYPTEGKPRNVAGAIIEHLFPKGQPSSIPIVSVTGTNGKTTVTRMIAHTLRVSGIT
                     TGMTTTGG"
BASE COUNT          700 a          587 c          733 g          476 t
ORIGIN      
        1 acgatatgct gtatgaatac ctgaaaatta tcgtcggtat ctgaccaaaa tccaacgatg
       61 ccggtaccga tgtccgggac tgccccgcac atgcggtacc ggcacagttc aaaattaacc
      121 cgccagggag acggtgaaat caatgggcac aggtaactta ataatcatag gaggcgctga
      181 ggataagaca ggtgaatgtc ttattctgaa ttcgtttgtc gagatttccg gggcatcgaa
      241 agcaaatatc gtgataataa ctacagccac ggaaaaaccc atcgaggcag gaagccagta
      301 caaatctttg tttatcggtc ttggcgcagg aaatgtgagc tttttgaaca tcaataccag
      361 ggaagaggcc gaatccgatc agaatgcgat gctcatagcg gaagccaccg gcatattctt
      421 caccggcggt gatcaactaa gaataacaag catactggga gggacaaaaa tttatgagac
      481 actgcaccag gcctggaaat cgggcgctgt catcgcaggt acgagcgccg gggcgtcggc
      541 catgagcagg acgatgatag ttgacgggaa cgataatgat ccggcaagaa aatgcacgct
      601 taagatggcg cccgggcttg gcttgctgga cagggcgctg atagaccagc atttccacca
      661 gcgagggagg atcgggaggc tgctttgcgg agttgctgaa aacccgtcga tcctggggat
      721 cggaatcgat gaagatactg cggtcagggt atttcctgac gatcatttcg aagtaatagg
      781 aagcaattcg gttatggtgg tggacggcaa gagcatcatg agttccaatg tatccgaact
      841 gatgccggac gaaaaccttg ccatcgccaa cgtcgtcctg catgtacttc ctgcaggata
      901 tggatatgat atggtcaaca gaaaggtctt gagggtggag gaaaaacagt ggaaataata
      961 aaaatagagc cgttcaaagg caggaacata tacagccaca ggcccgtgat cagggcggtg
     1021 gccgatcttg gagggcatta cgacacgccg accaggcaga tagaagggtt taatgaacgg
     1081 ctcctggaaa tgctgcctgg gcttgcgaaa catgtatgtt ccctcggtta cgagggaggt
     1141 tttgcagaaa ggctggcgga gggaacatac ctcgcacacg tggccgagca catggtactt
     1201 gaactccagg gcatgctggg cttcgacgtt cattatggga aaagcaggat tgcggaagaa
     1261 ccgtcgatat atcacatcat ttttgagtat aaaaatgaaa agacagccat agaatgtctt
     1321 ctggccgtat cagacatgat aaacgtgctc atcgccggca aaatgccgga tatcgtgagc
     1381 ataaaaaagc acctggaaag ggtcgcagcc gaatccggca tgggaccaag cactgaagca
     1441 ttgtatgaag aggcgcgcaa gaggaatatt cccgttgtgt gcctggagcc ttcaagcatc
     1501 ctgaggctgg gcgcgggcaa atacaccagg taccttgaag cttcgcttac agacagctgc
     1561 agctgcgttg ctgtcgatat ggcaggcaac aagcacctta cgaagatgaa gctttccgag
     1621 gccggcatac ctgtaccgcg gggagatgtg gcttactcct tcaggtctgc ggcagccatc
     1681 gcagcggcca tcggatggcc ggttgccgtc aaacctttcg atgccaacca gggaaagggc
     1741 gtctccacga acataacgag cttcgaagaa ctggaaaacg cattcaatgc cgcggccagg
     1801 ttcagccacg ccgttatagt cgaaaggcac atcgagggaa aggactacag gctgctggta
     1861 gtcggcggca aaatgatcgc ggctgcggag cgcagggcac ctttcgttgt cggggatggc
     1921 atacacacca tcagggagct tgtcgaaatc gaaaacctga acccgctgag aggtacggac
     1981 catgaaaagc cgctgacgat catcaggata gacgaaatgt cgaggcaggt acttgcgaaa
     2041 aacggctata ccgagaacag cataccagga gcaggcgtaa aggtattgct ccgctacaac
     2101 gggaacctga gcaccggcgg gactgcaagg gattgcacgg acgaagtgca tccgcataac
     2161 gcggaactgg ccgtaaaagc cgctgaactg atggaacttg acatagcggg gatagacatc
     2221 acctgcgccg atatatcgaa gcccctggaa acagccggtg gtgcggtcat cgaggtaaat
     2281 gcggcgcccg ggctgaggat gcacatatat ccgacggaag gaaaaccgag gaatgttgcc
     2341 ggcgcgataa tagagcacct tttcccgaaa gggcagccca gcagtatccc gatcgtatca
     2401 gtgacgggaa ccaacgggaa aaccaccgtg accaggatga tcgcgcatac attgcgtgtc
     2461 agcgggataa ctacgggtat gacgacaacg ggaggc
//
LOCUS       DAOSCC010000285         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_27643_length_2474_cov_3.033485, whole genome shotgun sequence.
ACCESSION   DAOSCC010000285 DAOSCC010000000
VERSION     DAOSCC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27643_length_2474_cov_3.033485"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1038)
                     /gene="noc"
                     /locus_tag="PLG72_11105"
     CDS             complement(178..1038)
                     /gene="noc"
                     /locus_tag="PLG72_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOJ81384.1"
                     /translation="MLESKLQFPKQAKENEKKNISYIDIDSIRPNPYQPRRQFSRESL
                     EELRESIQQYGVIQPINVRKISGNKYELVAGERRLRAAVMAGLKQIPAIVIDIDDNDS
                     AVLALIENLQREDLNYLEEAEGYSNLINEHGLTQEELARKIGKSQSTVANKIRLLRLP
                     PLVKKILAENNLTERHARALLKLHDEQLQLKVLRIVCEKGLNVKKTEELIERVIDKYL
                     GQGKDKDRSREKDKPADKKLTKTIKDIRIFVNTIRQAIELMRKSGVNARAAQFDRGEY
                     IEFIVRVPKA"
     gene            complement(1221..1937)
                     /gene="rsmG"
                     /locus_tag="PLG72_11110"
     CDS             complement(1221..1937)
                     /gene="rsmG"
                     /locus_tag="PLG72_11110"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOJ81385.1"
                     /translation="MDNMLAEYLKNGAAAYGAELDDRMTGMFFEYKRMLLEWNEKMNL
                     TAITDDREIILKHFVDSLSILPYVKGAGRIVDVGTGAGFPGIPLKIVLPALETVLLDS
                     LEKRIRFLEAVIGELGLSGAEAFHMRAEDAGRSETFRESFDIAVARAVASMPVLLEYC
                     LPLVKPGGIFIAMKGSSGDEVAASGKALEILGGSIQEVNDFVLPGSDMKRSVIIVRKV
                     RQTPTKYPRKAGKPSKEPLV"
     gene            complement(1930..>2474)
                     /locus_tag="PLG72_11115"
     CDS             complement(1930..>2474)
                     /locus_tag="PLG72_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81386.1"
                     /translation="RIEAEIERLENTYVPPSDEVNGFLESMGSTRIRSGASLAELLRR
                     PELDYRSLEAIDPGRPGSLRSSNTGKYDSVAEEVEISVKYDGYIKRQMMQVEQFRKME
                     RKKIPAGINYEEIYGLRLEARQKLSQVRPESVGQASRISGVSPADISVLLIYLNTRYG
                     REGRREDTGRDDGQAGERNG"
BASE COUNT          432 a          727 c          597 g          718 t
ORIGIN      
        1 tctattgttc tgccagatta tccctttaaa ttccctacag taactttacg ctaacgcaaa
       61 aaaagggctg ctctccggag agcagcacac aaaacagggg tcataatgtt gacacgcagg
      121 gtcctaagtg ggccttacgc tatattggct ttacctgatc gtccgggtcc gccaggctca
      181 agccttgggt acccttacga tgaactcgat gtattctccg cggtcgaact gggcagccct
      241 cgcattgacg cccgacttcc tcatcagttc gatggcctgg cggatcgtgt ttacgaatat
      301 cctgatgtcc ttgatggttt tcgtcagctt cttatcggcg ggcttgtcct tttccctgct
      361 cctgtccttg tcctttccct gcccgaggta cttgtcgatc acccgctcta tcagttcttc
      421 cgttttcttg acattaagcc ctttttcgca gacgatcctg aggactttga gctggagctg
      481 ttcgtcatgg agtttcagca gcgccctggc atgcctttcg gtgagattgt tttccgccag
      541 gatcttcttg acaagaggag gcaggcgcag gagccttatc ttgtttgcca cggtggactg
      601 gcttttccct atcttccggg caagctcctc ctgcgtgagg ccgtgttcat tgatgagatt
      661 gctgtatcct tctgcctctt cgagatagtt caggtcttcg cgctgcaggt tttcaatgag
      721 ggcaagcacg gcggaatcat tgtcgtctat gtctatcacg atggcgggga tctgtttcag
      781 cccggccatg accgcggccc tgagccgccg ctctcctgca acgagttcat atttgttgcc
      841 tgagatcttc cttacgttta taggctgtat cactccatac tgctgtattg attcgcgcag
      901 ttcctcgagg gactccctgc tgaactgcct gcggggctgg tagggattgg gccggataga
      961 gtcgatgtcg atgtagctta tgttcttctt ctcgttttct tttgcctgtt tcggaaattg
     1021 aagcttgctc tccagcatac agcaccatcc ttttccaacg cacgtcgtac catatgccgt
     1081 taggcggtac aacttcacgc aaatttacaa ttattacctg ttgactgcgt gaaatattat
     1141 tcgccgcctc ttttaggatt tccttttttt tataacaaga atcgttcgcc tctttttccg
     1201 aatttctggc agtaccttac ttataccaac ggctcttttg acggctttcc cgcttttctc
     1261 gggtattttg tcggtgtctg tcgcactttt ctcactatga tgacgcttct tttcatatcg
     1321 cttcccggca ggacgaaatc attgacttcc tgtatacttc cgccgagtat ttcgagcgct
     1381 tttcccgatg ccgcgacttc gtccccgctg ctgcctttca tggcgatgaa tatcccgccg
     1441 ggcttcacaa ggggcaggca gtactcgaga agcacgggca tcgaagccac agcgcgcgcc
     1501 accgctatat cgaacgattc cctgaacgtt tcggacctgc cggcatcctc ggcccgcata
     1561 tggaatgcct cggcgccgct gagcccgagt tcgcctatca cggcctcaag gaaccttatg
     1621 cgtttttcca gcgaatccag caggaccgtt tcaagggccg gaagtacgat tttcagcggt
     1681 atgcccggaa aacccgcacc cgtccccacg tcgacgatcc gccccgcccc tttgacgtag
     1741 ggcagtatgc tgagcgaatc cacgaagtgc ttgaggatga tctccctgtc gtccgtgatg
     1801 gccgtaaggt tcattttctc gttccattcg agcagcatcc tcttatattc gaaaaacatc
     1861 ccggtcatgc ggtcgtcaag ctccgctcca tatgccgcgg cgccgttttt gaggtattct
     1921 gccagcatgt tatccattac gctcacctgc ctgtccatcg tcccgtcccg tgtcttctct
     1981 ccggccttcg cggccgtacc gtgtgttcag gtatatgagc aggaccgata tgtcggcagg
     2041 ggatacgccc gagatgcggg atgcctgccc caccgactcg ggcctgacct gtgagagctt
     2101 ctgccttgct tccagcctta gcccgtatat ctcttcatag tttatgccgg cgggtatctt
     2161 tttcctctcc attttcctga actgctctac ctgcatcatc tgcctcttta tatagccgtc
     2221 gtacttgacg gagatctcca cttcttcggc tacagagtcg tatttccccg tgttggagga
     2281 tctgaggctt cccggccttc cggggtcgat ggcctcaagc gacctgtagt ccagttccgg
     2341 gcgcctcagc agttctgcaa ggctcgcccc ggaccttatc ctggtgcttc ccatggattc
     2401 aaggaaaccg ttgacttcgt cggacggcgg cacatatgtg ttttccagcc gttcgatctc
     2461 cgcctctatc cttt
//
LOCUS       DAOSCC010000286         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_27978_length_2452_cov_5.200250, whole genome shotgun sequence.
ACCESSION   DAOSCC010000286 DAOSCC010000000
VERSION     DAOSCC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27978_length_2452_cov_5.200250"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..1181
                     /locus_tag="PLG72_11120"
     CDS             528..1181
                     /locus_tag="PLG72_11120"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOJ81387.1"
                     /translation="MNPLGSSKEIVQNKLIIMYILQKLDMPVSNNALTKLILEMHLMN
                     YFVLQQCLNELCESNMASLVKDAPGTKNASERSLYEANASGLSAATDAPAAHDNRPEG
                     SSGSYILTDAGRKTLQYLITKIPSGIRKRLDNTIPAARKEIEKDLLITAEYIPESEDK
                     YLVICKMRERDFPLIELRATVGTKKDALSVCDNWKKHSSAIYSEIIESLTKDRSPDH"
     gene            complement(1167..1472)
                     /gene="yhbY"
                     /locus_tag="PLG72_11125"
     CDS             complement(1167..1472)
                     /gene="yhbY"
                     /locus_tag="PLG72_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOJ81388.1"
                     /translation="MLTGRQRSYLRSLANTMEPIFQVGKGGINDNLVKQFDDALEARE
                     LIKATVLRNSEFDAREAADEIAERTGADVVQVIGNRFVLYRESKKNRDISAALSMIR"
     gene            complement(1535..>2452)
                     /gene="cgtA"
                     /locus_tag="PLG72_11130"
     CDS             complement(1535..>2452)
                     /gene="cgtA"
                     /locus_tag="PLG72_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOJ81389.1"
                     /translation="GKGGAGNWHFATPTRQIPNFAKPGDPGEEYTVELELKMVADVGL
                     VGFPNAGKSTLLSKVTAAKPKIADYPFTTLEPNLGVVRVDEGASFLLEDIPGLIEGAH
                     RGVGLGFDFLRHVERCKMFLHIVDVAGVDGRDPVEDFEKINEELRLYDPKLAERYQVV
                     AANKIDLPQGKENLERFTREVEAKGYKVFPISAASNTGVWQLMQYIAAKLDELPVVVP
                     LTDTDEEVVYTAKEEEPFTIRREGNVFIVEGNWVDKLVKSVNFNSHESVQYFQRSLKT
                     KGVIDALEEMGINEGDTVRIYDIEFEYVR"
BASE COUNT          615 a          654 c          535 g          648 t
ORIGIN      
        1 ggaactgggt ttagcctcat aaacaatttt tggatttatt tttgctcatt tataggtata
       61 ttatttcccc gggaaataaa ggggtgggtg tacaattttt gaattgttgt gtgcagaaat
      121 ggcgtatata ttgataattt tcaataataa attctacata aaatcggtat aactgcttta
      181 tgtctcctgt cacatgaaaa agtgagcaaa cgtttttaca acaattaaaa aaatgtacac
      241 ccgcctctga ttggaagtca agaatatcgt ctgttaaatc cggaagatct tctgttaagt
      301 cctgaatatc gcctgcctgc ccgaagcatc gtctgatatc cggaacatcg tccgccaatc
      361 caagtatcgc ctgtcaaatc caaaacatct gccgattccg gggcattggt tccaattccg
      421 ttatccttgt cttcatgctt aatttatcca ggcaatattc tccgccggta caatatgcta
      481 taatttatac aatgatttac aaaacagttc ataccggggt gtaatggatg aatccacttg
      541 gaagcagcaa agaaatagtc cagaacaagc ttatcataat gtatatactg cagaagctgg
      601 atatgccggt cagcaacaat gcgctgacga agctgatact cgaaatgcac ctgatgaatt
      661 atttcgtgct ccagcaatgc ctcaatgaac tgtgtgaaag caatatggca tcgctggtga
      721 aggatgcgcc aggcaccaaa aatgcatcgg agcggtcttt atatgaagcg aacgcatcag
      781 gcctgtcggc tgccactgac gcaccggctg cacatgacaa caggccggaa gggtcgtccg
      841 gcagctacat cctgacagat gcaggcagaa aaacactgca gtaccttatc acgaaaatac
      901 cgtcaggtat aagaaagcgg cttgacaata caatccctgc cgcccgtaaa gagatagaga
      961 aagatctgtt gataacggct gaatacatcc ccgaaagcga agacaagtac ctggtgatat
     1021 gcaaaatgag agagagggac ttccccctca tcgaactaag ggcgaccgtc ggcacaaaaa
     1081 aagatgcttt atccgtctgt gacaactgga aaaagcactc ttcggccatt tactcggaaa
     1141 tcatcgaatc attgacaaaa gaccggtcac cggatcattg acaaggcagc gctgatatcc
     1201 ctgttctttt tggattccct gtacagcacg aacctgttgc ctatgacctg gaccacatcc
     1261 gccccggtcc tttcagcgat ctcatcggcc gcttcacgtg cgtcaaactc ggaattcctc
     1321 aggaccgtgg cctttatcag ttcacgggct tcaagcgcat cgtcgaactg ctttaccagg
     1381 ttgtcgttga tgccgccctt gcccacctgg aatatcggtt ccatagtgtt tgccaggctt
     1441 ctcagataac tcctttgcct gcctgtcagc atttggtttc ctccttgctg tattattcca
     1501 tccgtttatt gcaactgcct gcgggatagt cccatcatct aacgtactcg aattcgatgt
     1561 catatatcct gacagtatcc ccctcgttta tgcccatctc ctccagggcg tcgataacgc
     1621 cctttgtttt gagtgagcgc tggaagtact gtaccgactc atgcgagttg aaattgaccg
     1681 acttcacaag tttgtccacc cagtttcctt cgacgatgaa aacattgcct tccctgcgta
     1741 tcgtgaacgg ttcctcttcc ttcgcggtgt aaaccacctc ttcatccgtg tccgtcaaag
     1801 gaaccacaac agggagttca tcgagcttcg ctgctatgta ctgcatcagc tgccatacgc
     1861 ctgtgtttga ggctgcggat ataggaaaaa ccttgtagcc ttttgcttca acttccctgg
     1921 tgaacctctc caggttctct ttcccctggg gaaggtcgat cttgttggcc gccaccacct
     1981 ggtacctctc cgccagcttc gggtcataca gcctcagttc ctcgtttatt ttctcaaaat
     2041 cctccacagg gtcgcggccg tcaacacccg caacatccac gatgtgcagg aacattttgc
     2101 atctttcgac atgcctgagg aaatcgaacc caagcccgac tcccctgtgg gcgccttcta
     2161 taagaccggg tatatcttca agcaaaaagc tggctccctc gtctaccctt acgacgccca
     2221 ggttcggttc cagcgttgtg aacgggtagt ccgctatctt cggctttgcg gcagtaacct
     2281 tcgaaagcag cgtcgacttc ccggcattcg ggaatcctac aaggccgaca tcggcaacca
     2341 ttttcaattc aagctccacc gtatattctt cgccaggatc gcccggtttc gcaaaattcg
     2401 ggatctgcct ggtcggtgtg gcaaaatgcc agttgcccgc gccgcctttg cc
//
LOCUS       DAOSCC010000287         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_28367_length_2430_cov_4.322947, whole genome shotgun sequence.
ACCESSION   DAOSCC010000287 DAOSCC010000000
VERSION     DAOSCC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28367_length_2430_cov_4.322947"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /gene="hemW"
                     /locus_tag="PLG72_11135"
     CDS             <1..1372
                     /gene="hemW"
                     /locus_tag="PLG72_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOJ81390.1"
                     /translation="FNSYAGREHLAGSYFDALYKEIMLRSRRLGGRQVCSVFIGGGTP
                     SLVDPGYITKLIDVCREHFHVVDDAEISMESNPGTLTYDRLRAYRDAGVNRLSIGLQA
                     WQDRILKSIGRIHNRQQFVDNLKAAEECGFDNINVDLIFGLPGQTLGDWMETLEAVTG
                     FGPAKHVSGQSEHRPGPVKHISCYSLAIEEGTVFGDRLDARLLEPADDGLDREMYHRA
                     VDMLAEKGFRWYEISNFAVPGYECRHNLLYWKTREYAGFGAGAHSYLDAVRFNNVYGI
                     EEYIRAIDSVYKKYISSKYKYSRHGVGGAQGDTYIAAPGGKAKCGAGLAKPGFLEEMD
                     GLHENFDVIDKEGAMSEFMILGLRLTEGVSSAEFEERFGERLEDKYGGKLDMLAAKGL
                     IEAENMLAGTVGCGADRRSSEIPSGESCNADESCKEAGSCKPDRSYKLTRLGFDLANT
                     VFVEFI"
     gene            complement(1450..1902)
                     /locus_tag="PLG72_11140"
     CDS             complement(1450..1902)
                     /locus_tag="PLG72_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81391.1"
                     /translation="MKKICPVLICTCFLLFAAGCGNTDKLLDFTSSDVENIEIYMFNV
                     PINAEKKTVTGENDIKAITDRLNSVKIEREATPEDSMVGGGITFVFNKTDGTQFIINY
                     DGSTVKTPDDSPRRYWYKVDDDSLSGIWDDLDYPSQMVHQDELPTINS"
     gene            2324..>2430
                     /locus_tag="PLG72_11145"
     CDS             2324..>2430
                     /locus_tag="PLG72_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HOJ81392.1"
                     /translation="MIMDDRKKRILQAIIDDYIDKAEPIGSRTIARKYEL"
BASE COUNT          633 a          529 c          675 g          593 t
ORIGIN      
        1 cttcaattca tatgccggca gggaacatct tgccggttct tattttgatg cgctatataa
       61 ggaaatcatg cttagaagca ggcgcctggg cggccggcag gtctgttccg ttttcatagg
      121 cggcggaact ccctccctgg tcgatcccgg gtacataaca aagctgatcg atgtttgcag
      181 ggagcatttc catgtggttg acgacgctga gatcagcatg gaaagcaacc cgggcacact
      241 tacgtacgac aggctgaggg catacaggga cgcaggagtg aaccgcctga gtataggcct
      301 gcaggcatgg caggaccgca tcctgaaaag catcggcagg atacacaaca ggcagcagtt
      361 cgttgataat ctcaaagctg cggaagaatg cggctttgat aatataaatg tggatctgat
      421 attcgggctg cccggccaga cgctcgggga ctggatggag actctcgaag cggtgacggg
      481 attcggaccg gcaaaacacg tatccggaca gtcggaacac aggcccggac cggtaaagca
      541 tatctcatgc tacagccttg caatagaaga agggacagtg ttcggcgaca ggctggatgc
      601 ccggctgctt gagcctgcgg atgacggact cgacagggag atgtatcatc gtgccgtcga
      661 tatgctggcg gagaaaggct tcaggtggta tgagatatcg aacttcgcag tgccggggta
      721 tgaatgcagg cacaacctgc tttactggaa gacgagagag tatgccggtt tcggggccgg
      781 agcccattca tacctcgatg ccgtacgttt caacaatgtg tacggcatag aagagtacat
      841 cagagcgatc gacagtgtgt acaaaaagta tatttccagt aaatataagt acagcaggca
      901 tggcgttgga ggggcacagg gagataccta cattgcagca ccaggcggca aagcaaaatg
      961 cggcgccggc ttggcaaagc caggtttttt ggaagaaatg gacggcctcc acgaaaactt
     1021 tgatgtcata gacaaggaag gggcaatgtc cgagtttatg atcctggggc ttcggcttac
     1081 tgaaggcgtc agttcggcgg agttcgaaga gcggttcggt gaaaggcttg aagacaaata
     1141 tggcggcaag ctggacatgc tggctgcaaa agggctgatc gaagccgaaa acatgctggc
     1201 cggaaccgtc ggatgcggtg cggatcgccg cagcagtgaa atcccgagtg gcgaatcatg
     1261 taatgcagac gagtcatgta aagaggccgg atcatgcaaa ccggacaggt cgtacaaact
     1321 gaccaggctt ggattcgacc ttgcaaatac cgtattcgtt gagttcattt gaacagtttg
     1381 cacttaaagt gtattgcttt aagcagaaga tcccggctcc gcataaatgt tttcatggaa
     1441 atgatcatgt cagctattta tcgtgggcag ttcgtcctga tgaaccattt gggaaggata
     1501 gtccagatca tcccagatgc cggataaaga gtcatcgtct accttatacc agtaccggcg
     1561 gggtgaatca tcaggagttt ttacggtgct gccgtcgtaa ttgattatga attgggtgcc
     1621 gtcggttttg ttgaatacga atgtaatgcc ccctccgacc atcgaatctt caggggtcgc
     1681 ctctctctcg attttgaccg agtttagacg gtccgttatc gcttttatgt cgttttcacc
     1741 tgtaacagtc tttttttctg cgtttattgg gacattgaac atatagatct cgatgttttc
     1801 aacatctgac gaggtgaagt ccaggagttt gtccgtattt ccgcatccgg ccgcaaacag
     1861 caggaagcag gtgcatataa gaaccgggca gatttttttc acagtacgtg cctcctttta
     1921 tataccggcc agtttccggc tttcttcaga atttcggacg gttgcggttc gaggagttac
     1981 cagcctgctt ttgaatccgg gagatgcagt cttctttgta tttcagacgt tgcaggtttg
     2041 gtttggttcc cggattatat gtgaataaca aacgacgggt cgcaaacgcc gtattgcttg
     2101 atttttacag cctggaagaa cgctccgggg cgtgcctgcc ggggcaggcg ttttactgcc
     2161 ggcaatagat aaataaatct tgacaaaaaa aatccacggt ggtatgttta ttgtagatta
     2221 gcactcgcca agtcagagtg ctaacaaaca aaaataataa aattacgtat aatccgtata
     2281 aataatatat tgagtgattg tttattttcc aggggtgtat gagatgataa tggatgatag
     2341 gaaaaagagg attctccagg ccatcatcga tgactacatc gataaggccg aacctatcgg
     2401 ctcaaggaca atcgccagga aatacgagct
//
LOCUS       DAOSCC010000288         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_28523_length_2420_cov_3.690486, whole genome shotgun sequence.
ACCESSION   DAOSCC010000288 DAOSCC010000000
VERSION     DAOSCC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28523_length_2420_cov_3.690486"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1401)
                     /locus_tag="PLG72_11150"
     CDS             complement(223..1401)
                     /locus_tag="PLG72_11150"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ81393.1"
                     /translation="MDAVWKDRVRKALSREIGEGGIAGANLLVIRKGKEIFYHEDGFA
                     SIEKKLPVKRDTIFRIYSMTKPVTAAAVMILLERGEIDLFDPVSRFLEGFRGQMVCEG
                     SSLVPAEREVNIRDLLSMTSGLVYPGEDMAGRQVGILFQALEAGLSGGDPIGTVEAMN
                     RLGGCPLAFQPGTSWQYGTSADVLGAVAEVVSGMMFGEFLEKEIFGPLGMKDTAFWVP
                     AENQHRMASVYSSDEKGGLSLYTGNNLAVSNKMDKPPAFESGGAGLASTIDDYSRFAM
                     MLMNEGELDGVRILQPKTVRFMTGAALNDIQQKAFEKWMTLSGHSYGNLMRVMKDCSL
                     AGIIGSPGEYGWDGWLGTYFCNCPKEGLTFLLMMQKTDAGTTSLTRKVRNIILGSLTD
                     "
     gene            complement(1470..>2420)
                     /locus_tag="PLG72_11155"
     CDS             complement(1470..>2420)
                     /locus_tag="PLG72_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOJ81394.1"
                     /translation="GIPRIAAHNRMKIKGADVTADEIADPYSICRWNPDMYGLNVGAD
                     GAQQYYDSLFELYAQWGVDFVKVDDICRRDMPSAKKEIEMIHHAIRRCGRPIVLSLSP
                     GPALVDQAWHYKKYANMWRITDDFWDRWPLLLNMFERCEMWQDHVAPGCWPDCDMLPL
                     GYIGKGFGQERYTNFTRDEQVTMMTLWCMFRSPLMLGAELTKLDEWTGSLITNAKVLR
                     LLTHSCGARQIMRDGGQAVWVSEDTDGRGIYIAVFNLGGKERRVTVCAEELELTGIDS
                     FTGMKFEELWSGDIAINTGDVLETAVPSHGARLFRIERPV"
BASE COUNT          499 a          758 c          548 g          615 t
ORIGIN      
        1 ttcgcctcat aacgcgccgg tccatacacc atgcagcgct ccgacagtgc ctcggtttgc
       61 tgcaaaccgc ccggatcacc gcggatcccg gcgaagtctt tcgtccgtcg gccggatatt
      121 cggcgtcagt cgctgtttta ttcgatttgc tcattacagg cttcgtccgg agatgatcct
      181 cgggactcag catataaagc ataccatatt gtcaactttt tgtcaatctg taagcgaacc
      241 cagtattata ttccttacct tcctggtaag cgaagtcgtt cccgcatctg tcttctgcat
      301 cataagcagg aatgtcagcc cctccttcgg acagttgcag aaatatgtcc caagccagcc
      361 atcccagccg tattccccgg ggctgcctat aatgcctgca aggctgcaat ccttcatgac
      421 ccgcatcagg ttgccgtagc tgtggccgct gagcgtcatc catttctcaa aggctttttg
      481 ctgtatatca ttgagagcgg ctcccgtcat aaacctgacc gtcttaggct gcagtatcct
      541 cactccgtcc agttcgccct cattcatcag catcatggcg aacctggagt aatcgtctat
      601 cgtggaagcc agccctgcac ctccggattc gaacgccggc ggcttatcca ttttgttgct
      661 tacggcaagg ttgttgcctg tataaagcga caggccgcct ttctcatcgg atgaatatac
      721 ggaggccatg cggtgctgat tctccgcagg cacccagaat gccgtgtcct tcattcccag
      781 gggcccgaat atctccttct caaggaactc gccgaacatc atgccgctga cgacctccgc
      841 aacggcgcca aggacgtcgg cggatgtccc gtactgccat gaagttcccg gctggaatgc
      901 cagcggacat ccgcccagcc tgttcatggc ctcgacggtg ccgatgggat cgcctccgga
      961 cagcccggct tcaagtgcct ggaacagtat tcccacctgc cggcctgcca tgtcttctcc
     1021 tggataaacc aggcccgacg tcatggaaag cagatccctt atattgactt ccctctccgc
     1081 aggtacaagg ctgctgccct cacagaccat ctggcccctg aagccttcca ggaacctgct
     1141 caccggatcg aacagatcta tttccccgcg ctccagcaaa atcataaccg cagcggcagt
     1201 gaccggcttt gtcatcgaat atatcctgaa aatagtgtcc cttttgacgg gaagcttttt
     1261 ttcaatactt gcgaaaccat cttcatggta gaatatttct tttcccttac ggatcaccag
     1321 aagattagcg ccggcgatgc cgccttcacc gatttccctg cttaatgcct ttcttacacg
     1381 gtctttccat actgcgtcca gcacaagatc atctccctgt ctattttata taaactgata
     1441 atggaagtca tgctgtcctg ccggtcgggt catacaggcc tttcgatcct gaatagcctt
     1501 gctccgtgag acggtaccgc agtttcaagg acatcgccgg tattgatcgc gatatcgccg
     1561 ctccacagtt cttcaaactt catgcctgta aacgaatcaa tgcccgtcag ttccaactct
     1621 tctgcacaga cagtgaccct tctttctttg ccgcccaggt tgaaaacagc tatatatatg
     1681 cccctgccgt ccgtatcctc agagacccac acagcctgtc cgccatccct cattatctgc
     1741 cttgctccgc atgaatgggt caggagcctg agcactttgg cgttcgttat gagcgatccg
     1801 gtccactcgt cgagcttcgt aagttcggcc ccgagcataa gcggcgatct gaacatgcac
     1861 cacagcgtca tcattgtgac ctgttcatct ctcgtgaagt tcgtatacct ttcctggccg
     1921 aaccctttgc ctatgtaccc cagcggcagc atgtcgcagt caggccagca tcccggcgca
     1981 acgtggtcct gccacatttc acaccgttcg aacatgttca ggagaagcgg ccatctgtcc
     2041 cagaaatcgt ccgttatcct ccacatatta gcgtattttt tatagtgcca tgcctggtcc
     2101 acaagtgccg gccccggcga aaggctcagg acgatcggcc gtccgcatcg ccttatggcg
     2161 tgatgtatca tttctatttc tttctttgcc gacggcatgt cacgcctgca tatgtcatcg
     2221 actttcacga aatcgacgcc ccactgtgcg taaagctcga aaagagaatc ataatactgc
     2281 tgtgcgccgt ccgctcccac attcaggccg tacatatccg ggttccaccg gcatatcgaa
     2341 tacgggtcgg ctatttcatc ggccgtcaca tctgccccct tgatcttcat gcggttgtgc
     2401 gccgcgatcc tcgggatgcc
//
LOCUS       DAOSCC010000289         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_29584_length_2357_cov_4.724153, whole genome shotgun sequence.
ACCESSION   DAOSCC010000289 DAOSCC010000000
VERSION     DAOSCC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29584_length_2357_cov_4.724153"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLG72_11160"
     CDS             complement(<1..690)
                     /locus_tag="PLG72_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOJ81395.1"
                     /translation="MPLVTTTEMFKKAYEGGYAIGAFNVNNMEIVQGITEAAKEVNAP
                     LILQVSSGARKYAKHTYLVKLVEAAIEETGLPICLHLDHGDSFELCKSCIDGGFTSVM
                     IDGSHLPFEENIKLTKQVVDYAHAHGVVVEGELGRLAGIEDAVNVSDEDAAFTKPEEV
                     EEFVGRTGVDSLAIAIGTSHGAYKFKGEPKLRFDILEEVARRLPGFPIVLHGASSVIP
                     EFVEKINKYGGN"
     gene            complement(999..1520)
                     /locus_tag="PLG72_11165"
     CDS             complement(999..1520)
                     /locus_tag="PLG72_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ81396.1"
                     /translation="MLQILPAIIIIVAVIAAWTVFTQRKLVMLDENISNAMSLIGAQM
                     SSRFDVLMFLLELAKGYAVTGCEELMETVRAERGVIMAKSAPDDVIQQEGVISDVFGK
                     IVIASDRNPKLTADPNYIKAMDAMRTIENMMHTSRLIYNDSAARLNREIRMFPASVLA
                     RILGLRQRDYLAE"
     gene            1743..>2357
                     /locus_tag="PLG72_11170"
     CDS             1743..>2357
                     /locus_tag="PLG72_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ81397.1"
                     /translation="MLQIAVCDDEISQLSQIMQYINQYRTSRNLNCEYAVFTNGFELI
                     SAIEKGKKFDVYCLDIIMPGFTGIDVAKEIRAHDKNSMILFFTSSSEFALESYSVKAI
                     DYILKPVTKEKLFSAFDEMLEHVKDRENEDAIVLKSSEGIRRILITNLAFVEVIGRNV
                     LYHLRSGSVAECKEAFSSACDRLLKYRCFLKPHRSYLVNMQYIDT"
BASE COUNT          619 a          580 c          528 g          630 t
ORIGIN      
        1 attgccgccg tacttgttta tcttttcgac gaattccggg atgactgaag aagcgccatg
       61 gaggactatc gggaacccgg gcagtctcct tgcgacttct tcaaggatat cgaacctgag
      121 tttaggttcg cccttgaact tgtaagcgcc atggctcgta ccgatcgcga tcgcaagcga
      181 gtcgacgcct gtcctgccca cgaattcttc gacttcttca ggctttgtga aagcggcgtc
      241 ttcatcggat acgttgacag catcctcgat tcctgccagc ctgcccagtt cgccttcaac
      301 gactacgcca tgggcatgcg cgtaatcaac gacctgcttt gtaagcttta tgttctcttc
      361 gaaaggaaga tgtgaaccgt ctatcatgac agaagtgaaa ccgccgtcta tgcatgattt
      421 gcaaagttca aagctgtcgc cgtggtcaag gtgcaggcag atcggaaggc ccgtttcttc
      481 gatagcagcc tcaaccagct ttacaagata agtatgtttc gcatatttcc tggcgcctga
      541 cgacacctgc aggatcagcg gagcattgac ttccttcgcg gcttccgtga ttccctggac
      601 gatctccatg ttgttgacgt tgaaagctcc tatggcataa ccgccttcat aggccttttt
      661 gaacatctca gtggttgtaa ctaatggcat aactaacaac tccttttctt atatagatta
      721 atattgctta acggtcgtaa aaaaatgtac ttcggacaca tcatcccctg tactgaaaat
      781 ccactgcctg tgtgtccggc aaagcggcag atattcgcca cctcgttata aatttgtcat
      841 attcattcta ccagatttaa ggtgaaatgc aagtgccaaa cacgtttttt aaacgtgaaa
      901 aacgtcgctg ggtacggcaa aaaggcagtt gttgtcggta aaaagctatg ccgcggatat
      961 acgatatgac aggggcatcc ggcatatatc cgcttccctc attccgccag ataatcccgc
     1021 tgcctgaggc caaggatcct tgcaagcacg gatgccggaa acatccgtat ttcacggttc
     1081 agcctggccg cactgtcgtt gtagatcaaa cggctggtat gcatcatgtt ttcaatggta
     1141 cgcatggcat ccatggcttt gatgtaattc ggatccgccg ttaatttggg gttccggtcc
     1201 gatgcaatca ctatcttgcc aaaaacatcg gaaataacgc cttcctgctg aatcacatcg
     1261 tccggtgcgg actttgccat tataacgcct ctttctgctc tgaccgtttc catcagttct
     1321 tcacatccag ttacagcgta accttttgcc agttccagaa ggaacatcag tacgtcgaac
     1381 cggcttgaca tctgcgcccc gatcaggctc attgcattgc tgatattctc atcaagcatc
     1441 accagtttgc gctgcgtaaa gacggtccac gcggcgatca cagcaacgat tattataatt
     1501 gccggtaaaa tttgcagcat aataaatcac tcctgtctat aatgtatggg caggctgtta
     1561 atttccgagg aacagcccct gtttcaattt aaccgaatta tcctggcaaa aacctccttt
     1621 tgcatggaac atgatttttt cgacatgaaa catgtatttt gaccggaagt gaaaaacagt
     1681 gtttcgcaat agagcattat ggtaaactat taacatggcg ccaatcgaac ggaggaacac
     1741 acatgctgca gatcgcagtc tgcgacgacg aaatcagtca attgtcacag atcatgcagt
     1801 atattaacca gtacaggaca tcaaggaacc tgaactgcga atatgccgtt tttacaaatg
     1861 gctttgaact gatttccgca attgaaaaag gaaagaagtt cgacgtctat tgccttgata
     1921 taatcatgcc tggatttaca ggtatcgacg tcgcgaaaga gatccgcgca catgacaaaa
     1981 attcgatgat cctgtttttc acatcatctt ccgaatttgc cctggaaagc tattcggtaa
     2041 aggcgatcga ctatatactc aagcccgtaa cgaaagagaa actgttttcc gcatttgatg
     2101 aaatgctgga acatgtaaaa gacagggaaa atgaagatgc tatcgtgctg aaaagcagcg
     2161 aggggatccg gagaatactg ataaccaacc tggcctttgt cgaggtcatc ggccggaatg
     2221 tgctgtacca cctgcgctcc ggcagcgtgg ctgaatgtaa ggaggctttt tcctctgcct
     2281 gcgacaggct cctgaagtat aggtgttttc tcaaaccaca ccgctcttat ctcgtaaata
     2341 tgcagtatat agatacc
//
LOCUS       DAOSCC010000290         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30086_length_2329_cov_3.814424, whole genome shotgun sequence.
ACCESSION   DAOSCC010000290 DAOSCC010000000
VERSION     DAOSCC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30086_length_2329_cov_3.814424"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLG72_11175"
     CDS             <1..949
                     /locus_tag="PLG72_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81398.1"
                     /translation="ALGCIVLSKVYSERIRNYEEFIFPVFGWRIGWVIETAAIVFIFC
                     MYSIMTAGAGNILAQLTGMPFGTAVLIMGIISAILIMTDIKGIALFSSVLTPVLIAGI
                     IIAGLCIIIMKDSPAFSVSGYFSRITNNWFVSSILYVSYNSILAVMMLCSVQPYLKTR
                     RTAVAAGILGGTALALAALVINTVIFLFYPSASDREIPIIDILQHISASAGRLYSVLL
                     WLAMLVSAVTSGFCFTDRIYSLFRLDRRITALLLCVCAVPLSTLGFSRLISLIYPAFG
                     WLGLFMVIVIMITGLQKPSRDPGRDEYGNAERSLRAGGK"
     gene            1044..2210
                     /locus_tag="PLG72_11180"
     CDS             1044..2210
                     /locus_tag="PLG72_11180"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOJ81399.1"
                     /translation="MKYIPEQDRPAPGGRAAGIITALMLFLCIAAVAVFSYKRSLDPS
                     IDLQDTVTRLAGLGEREAKAAEVQYSYTFDAREKPVFTLYRGNIVKSSRSGILFFDKT
                     GVIVRSESIGFDDPIVRTNGSRLLVVDSGATGICVMDSETIRWQDRTDTAILNADISD
                     DGYVAVIAEAKRDNNVVRVYESHGVELFRKIIATGYAVSACISPSKKHLVLSSVSTGV
                     VGPFSRYKFYDMEGNELTEISFDSSGDLLPLFWFNRDDSIFAVGDSSAAYISPDGKVG
                     WTERFRSVAGAAPAGDGRLAVAAQADEGAVLNVYTAEGEKDVSVMLRGMPKGLDAARG
                     MVAVYTEDTVYFYDDRGVNTCIYDADDKILEVRLFDKRQAAVITEGEITVIGIE"
BASE COUNT          534 a          571 c          676 g          548 t
ORIGIN      
        1 cgctttgggc tgtatcgtcc tcagcaaggt atacagcgag cggataagga actacgagga
       61 attcatattc ccggtgttcg gatggcggat cggctgggtc atcgagacag ccgccatcgt
      121 gttcattttc tgcatgtaca gcataatgac ggcaggtgcg gggaacatac tcgcgcagct
      181 gaccgggatg ccgttcggga ccgcagtgct tataatgggt atcatcagtg ccatattgat
      241 aatgacggac attaaaggca tcgcattgtt cagttcggtg ctcacgcctg tactgatcgc
      301 aggcatcatc attgcaggtc tctgtataat aatcatgaag gacagccccg cattcagcgt
      361 atcaggatat ttttcgcgca ttacgaacaa ctggttcgtt tcatcgatcc tttatgtcag
      421 ttacaacagt atcctcgcag tcatgatgct gtgcagcgta cagccatacc tgaaaacgcg
      481 caggacggcc gtggcggcgg ggatacttgg cggcaccgcg ctggccctgg ccgctcttgt
      541 gatcaatacg gtcatttttc tgttttatcc ttcggcttca gacagggaaa taccgatcat
      601 tgatatcctg cagcacatca gcgcgtcggc ggggcggctt tacagcgtgc tgctgtggct
      661 tgccatgctg gtatccgccg tgacttccgg cttttgtttc actgacagga tatacagcct
      721 gttcaggctg gacagaagga tcactgcatt gttactgtgc gtatgtgcgg tgccattgtc
      781 gacacttgga ttttccaggc tcatatctct tatttatccc gcgttcggct ggcttggcct
      841 gttcatggtc atcgtgataa tgatcactgg tcttcagaaa ccgtcgcggg atcctggacg
      901 cgatgagtac gggaatgcgg aaagaagcct ccgtgccggc gggaaatagc gctttgcggc
      961 agcctgtctc tctttcaatg gccggtttta tatggtaaaa tgttttgtac aggtaatacg
     1021 gaagacaaag ctgaggtggt cttttgaaat acattcccga acaggacaga ccggcgcccg
     1081 gaggacgcgc agccggcatc atcacggcgc tcatgctttt cctgtgtata gctgcggtag
     1141 cagttttcag ctataagcgc agcctggacc cgtcgatcga tctgcaggat acggtgacca
     1201 ggctcgccgg tttgggagag agggaggcaa aggctgccga agtgcagtac tcctatacgt
     1261 tcgacgccag ggaaaagccc gttttcacgc tgtacagggg aaatatcgtg aagtccagca
     1321 gaagcggcat attgttcttc gataaaaccg gcgtgatcgt gcggtcggaa agcatcgggt
     1381 tcgacgatcc catagtcagg accaacggat cgaggctcct tgttgtcgat tccggagcaa
     1441 caggcatatg tgtcatggac agcgaaacga tacgatggca ggacaggacg gacactgcca
     1501 tccttaatgc agacataagc gatgacggat acgttgctgt cattgctgag gcgaaaaggg
     1561 acaacaacgt ggtaagagtc tatgaatcgc acggagtgga gcttttccgc aagataatag
     1621 cgaccggcta tgccgtttcc gcatgcatat cgccgtcaaa gaagcacctg gttttgtcat
     1681 ctgtctcaac gggcgttgtc ggcccgtttt cgcggtacaa gttctacgac atggaaggga
     1741 atgaactgac ggaaatatca tttgattcat ccggcgacct tctgcccctt ttctggttca
     1801 acagggacga cagcatattt gccgtcgggg acagttccgc tgcttacata agccctgacg
     1861 ggaaggtcgg atggacggaa cggttcagga gcgtcgcggg ggcggcccct gccggagacg
     1921 gcaggctcgc ggtggccgcg caggcagatg aaggcgcggt cctgaatgtc tacaccgctg
     1981 agggcgaaaa ggatgtttcc gtaatgctcc ggggcatgcc gaaagggctg gacgccgcaa
     2041 ggggaatggt tgccgtctat actgaagata ccgtatattt ttatgacgac cgcggcgtca
     2101 acacgtgcat ttatgatgca gacgataaaa tactcgaggt ccgcctcttt gacaaaagac
     2161 aggctgctgt gatcacagaa ggcgagatca cagtcatagg gatagagtag gacattcggg
     2221 acggttccca cgtccggaca tgcgggcgat ttctgcgccc tggtcacaat gataccgtct
     2281 cagacactgg aacggttcct gcgtacatat gaacaaatgg aacggaaag
//
LOCUS       DAOSCC010000291         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30388_length_2313_cov_5.352524, whole genome shotgun sequence.
ACCESSION   DAOSCC010000291 DAOSCC010000000
VERSION     DAOSCC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30388_length_2313_cov_5.352524"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLG72_11185"
     CDS             complement(<1..459)
                     /locus_tag="PLG72_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOJ81400.1"
                     /translation="MSGGYAKLNGTDCGFMGIREIRSGVAGGKYSASEITGMYIEKIK
                     AMDDEIGAFLTICEESAAEQAKAVDEKIRSGKEPGRLAGVPVAIKDNICTDGIRTTCA
                     SRMLENFIPPYNATVIEKLMSEDAIIIGKTNMDEFAMGSSTENSAFKITKN"
     gene            complement(474..761)
                     /gene="gatC"
                     /locus_tag="PLG72_11190"
     CDS             complement(474..761)
                     /gene="gatC"
                     /locus_tag="PLG72_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOJ81401.1"
                     /translation="MKISKDDVVYIAKLAKLRFSDEEIEKLTVEFESILSHFQSIDRM
                     DLDNVDPDEFADRRESVLRADENVTFGDKEKLFQNAGSMRGTFIQVPRILE"
     gene            complement(952..2241)
                     /gene="aspS"
                     /locus_tag="PLG72_11195"
     CDS             complement(952..2241)
                     /gene="aspS"
                     /locus_tag="PLG72_11195"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOJ81402.1"
                     /translation="MKRSCVADVLANDAQKVLVRGWVHNINRIGKIAFVQLRDRTGII
                     QIVADDDMVRDLRLEMSIEAAGNRVLNEKAPYGVEILADSIRLLGTVYYDMLPFEINR
                     KKIKASLETQLDHRTISLRPVKSRAIFKVQQEIEEVFRNYLKENGFTQIHTPKIISSA
                     TEGGSEMFTVDFFGKRAFLAQSPQFYKQMMVGAGIERVFEVGHAYRAELHNTWRHLSE
                     YVSMDVEMGFIESYEDLMDLEEGYINRLFSHLKSACAGELEMFGVSLPDSVEIPRIPL
                     AEAQRIVFEKYGKRSPASGLDAEGEKLFSDYMRNEHGTDFVFLTKYPASKRPMYTMPD
                     PEDPELTQSFDLIYKGLEITTGSQRIHDYEMLRRNIIKFGLDPECFSFYLDSFRYGMP
                     PHGGFAIGLERITMKILDLDNIREAALFPRDIRRLEP"
BASE COUNT          493 a          680 c          472 g          668 t
ORIGIN      
        1 gttctttgtg attttgaatg cggaattctc agtggacgaa cccatcgcga attcatccat
       61 gtttgtcttc ccgattatta ttgcgtcctc gctcattaac ttctcaatga ccgttgcatt
      121 atatggcggg ataaagtttt caagcatcct ggatgcacag gtggtcctga tgccgtcggt
      181 acatatgttg tccttgattg cgacaggcac tccggccagt cttccgggct cttttccgct
      241 tcttatcttc tcgtccactg cttttgcctg ttcagcggcg ctctcttcac aaatagtcag
      301 aaatgcacct atctcgtcat ccatagcctt tattttttca atatacatcc ctgtgatttc
      361 agatgccgaa tacttcccgc cggcaacacc ggatcttatc tcacgtatgc ccataaaacc
      421 gcagtccgta ccatttaact ttgcataacc gccgctcatc cgaacacctc ccgttactcc
      481 agtatcctgg gaacctgtat gaatgtcccg cgcatggatc ccgcattttg gaacaatttc
      541 tctttgtccc cgaaagtcac gttttcatcg gctctgagca ccgattcccg cctgtcggcg
      601 aactcatccg gatccacgtt atcaagatcc atcctgtcga tggactggaa gtggctgagt
      661 atgctttcga actccactgt cagcttttcg atctcctcat cgctgaagcg aagctttgca
      721 agctttgcga tatagacaac gtcatctttc gatatcttca taggccgcac tcctccgcgt
      781 tataaattca tgcaccggtt cttaaccatt gtttgctatt attccggtta tcaatacatc
      841 cggcctgcct ggcaacggtt cctcccggca gcattccatc cggtcagcgt ttgctccggc
      901 taaaaaccgt cacttccagg cataatacca ttgccggata ttctccatcc tctacggttc
      961 cagccttctt atgtccctcg ggaacaatgc cgcttcgcgt atgttgtcaa gatccagtat
     1021 cttcattgta atcctctcaa ggccgatggc aaatccgcca tgcggcggca tgccgtacct
     1081 gaagctgtcc aggtagaagg agaagcactc aggatcaagg ccaaacttta tgatgttccg
     1141 cctgagcatt tcataatcat ggatcctctg gctgcccgtg gttatttcaa gtcctttata
     1201 tatcagatcg aagctctgcg tcaactcggg gtcctcagga tcgggcatcg tatacattgg
     1261 cctcttggat gccggatatt tcgtcaggaa gacaaaatcc gtaccatgtt cattcctcat
     1321 ataatccgaa aacagcttct cgccttctgc gtcaagaccg ctggcgggcg accgcttgcc
     1381 gtacttctcg aacactatgc gctgggcttc cgccagcggg atcctgggta tttccacgct
     1441 gtcgggcaaa gacacgccga acatctcaag ttcaccggca caggccgatt taaggtgact
     1501 gaacaggcgg ttgatatagc cttcctccag gtccataagg tcttcatagc tctcaatgaa
     1561 gcccatttcc acatccatgc tcacatattc gctcagatgc ctccatgtat tgtgcagttc
     1621 cgccctgtat gcatgcccca cttcaaacac cctttcaata cctgcgccga ccatcatctg
     1681 cttgtaaaac tgcgggctct gggcaagaaa cgccctcttt ccgaagaaat ccaccgtaaa
     1741 catctcggat ccgccctctg tcgcgctgct tattatcttg ggagtgtgga tctgggtaaa
     1801 tccgttctct ttaagatagt tcctgaaaac ttcttcgatc tcctgctgaa ccttgaatat
     1861 cgcacggctc ttgacaggcc tcaggctgat ggtcctgtga tccagctggg tttcaagcga
     1921 agcctttatc ttcttcctgt tgatctcgaa cggcagcatg tcatagtaga ctgttccgag
     1981 taatctgatg gaatctgcaa gtatctcgac tccgtaaggg gccttttcgt tcaaaaccct
     2041 gtttccggcc gcctctatgc tcatttcgag cctcagatcc ctgaccatgt cgtcgtcagc
     2101 cacgatctgg attatgccgg tcctgtccct caactgtaca aatgctattt tcccgatacg
     2161 gtttatattg tgcacccaac cccttaccaa tactttttgt gcatcgttcg caagcacatc
     2221 ggccacacac gatcttttca caaccatcgc ctcctttttc gatttttgcc cggcccaatg
     2281 tcccggtctt atacctcagt catatacatc agc
//
LOCUS       DAOSCC010000292         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30818_length_2291_cov_4.168157, whole genome shotgun sequence.
ACCESSION   DAOSCC010000292 DAOSCC010000000
VERSION     DAOSCC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30818_length_2291_cov_4.168157"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLG72_11200"
     CDS             complement(<1..384)
                     /locus_tag="PLG72_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81403.1"
                     /translation="MKKDVNSEGHKRPESKKWKVAFYIFLIAAFASVTTYAGIKLGPR
                     VTELARKPEEFRDWLNSFGWKGVLVFIGVQVLQVVVAAIPGEVTQLAGGYLYGTFFGT
                     IYSLAGIVLGSVIVFYAARLLGYPLV"
     gene            complement(1033..>2291)
                     /locus_tag="PLG72_11205"
     CDS             complement(1033..>2291)
                     /locus_tag="PLG72_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ81404.1"
                     /translation="RLYKLTPLEDSFSCNFNVLISGTPCVMGIRSILKEWVSFRVDCI
                     KRQTLFDIEKKEAKLHLLLGLRKILLDIDKAIAIIRNTENDEQVVPNLMEGFGIDKLQ
                     AEFIAEIKLRNLNREYILDRVGEVDTLQKEIVDLKDIYGSEKRIKQIIMKQLGDVAKK
                     YAQPRRTEIICETSIEEITQEHMIEDYNLKMFLTEQNYIKKIPLVSLRSNPEHKLKED
                     DTIIQELETHNKAELLLFSNRQTVYKMRIYDIPDCKASSLGEYLTNMLQLEEDERIIF
                     MTATDNYSGWLLFSFENGKCAKITLESYATKTNRKKLANAYSDLSPLVDIRYLAEDTD
                     LVAYSSINKVLVFNTAAINPKTTRDSQGVQVMKSKKGSRMCSVKTIEESGIRNPDYYR
                     TKVIPAIGCYLRAEDLEDKQITIDCT"
BASE COUNT          505 a          607 c          489 g          690 t
ORIGIN      
        1 gaccagcgga taacccagca gacgcgccgc atagaatact atcacggatc ccagaacgat
       61 tcctgccagc gaatagatgg tgccgaaaaa tgttccgtac aaatagccgc cggccaactg
      121 tgtgacttct ccggggatcg cggcaacgac aacctgcagc acctggaccc cgataaatac
      181 gagtactcct ttccagccga atgaattaag ccaatctctg aattcctcag gtttcctggc
      241 aagttcggta accctcggcc ccagtttaat gccggcataa gtagttactg aagcaaaggc
      301 tgctattaaa aaaatataaa acgcaacttt ccatttcttg ctttccggcc ttttatgtcc
      361 ctctgagttc acgtctttct tcatcgtttt tcaaacctgc ttttagtgtg ttgatttata
      421 tatcattttt acccggaagt gctaagaaac tgccggatta gctaaggctt ctcttaacat
      481 cccaataata ttatgatgca tatccgccat tgtcagtcat tggtatatca caatgttatt
      541 aatccacgac cgtgacctga tatatggtta tcatcgctgc cgcaattgcc gatatgctca
      601 gcatgatttc accgtgtgtc ccacacagct ccaccgtatt tttactttgg gttgccgcgc
      661 cgtccggatt cgcatatcgc ctcaaggccg acctgacttg ggaactctat gaacacctac
      721 cggaggagat attgaaacat ctcggcctgg gtttggccct cggcctcccg aagggaccga
      781 atccgcgcac aaactgcatg tatattccgt cacttgtgtt cctttaccaa gtgccagccc
      841 gccaggcagg ccgccgtgca attcacattc atccatctgc tcaacattca tccatctgct
      901 caccctgatg cagttcgcct ggtcggaggc atttacatac gtttataccg cttctcacat
      961 gcaattacat tatgtgtaat ttcactgtcc gtaatttact gcaatcagtt tgctgtgtcc
     1021 cgtcgacacc tgttatgtgc agtctatcgt tatctgcttg tcttccagat cttccgccct
     1081 cagatagcat ccgatcgccg ggatgacttt agtgcggtaa taatcagggt tcctgatacc
     1141 cgactcctcg atagtcttca ctgagcacat cctgctccct ttcttcgatt tcatgacctg
     1201 gaccccctgc gaatccctgg tggttttggg attgatggcc gcagtattga acaccagcac
     1261 cttgttgatg ctgctgtatg caaccaggtc tgtatcctct gcgagatacc ttatgtcaac
     1321 cagaggcgag agatccgaat atgcatttgc aagctttttc ctgttggttt ttgttgcgta
     1381 gctttccagc gtgatcttgg cgcatttgcc gttttcaaag gagaacagca gccagccgga
     1441 gtaattgtcc gtagccgtca taaaaattat tctttcgtct tcctcaagct gcagcatatt
     1501 cgtgaggtat tcgcccagtg agcttgcttt gcagtccggt atatcgtata tacgcatctt
     1561 gtataccgtc tgtctgtttg aaaaaagaag cagttccgcc ttgttgtggg tctcgagttc
     1621 ctgtattata gtgtcgtcct ccttgagctt gtgctcggga ttggaccgca gcgataccag
     1681 cgggatcttc ttgatgtaat tctgctctgt aaggaacatt ttgaggttgt aatcctctat
     1741 catgtgctcc tgggtaatct cttctatgct cgtctcgcaa ataatctctg tccttctcgg
     1801 ctgcgcatac ttcttggcta cgtcgccaag ctgcttcatt atgatctgct ttattctctt
     1861 ttcgctgcca tatatatctt tcaggtcgac gatctccttt tgcagcgtgt ccacttcgcc
     1921 taccctgtcc aggatgtatt cgcggttcag gttcctgagc ttgatttcag caatgaattc
     1981 agcctgcagc ttatctatgc cgaagccttc catgaggttg ggaacgacct gttcatcgtt
     2041 ttcagtattc cggataatcg caatggcttt gtcgatatcg agcaggatct tgcgcaagcc
     2101 aagcaacagg tgcagtttgg cttctttttt ttctatatcg aacaacgttt gcctctttat
     2161 acagtcgacc ctgaacgaaa cccattcctt cagaatcgat ctgatcccca ttacacaggg
     2221 agtgccgctg atgagcacat tgaaattgca actgaaggag tcctccagcg gagtcagctt
     2281 gtaaagcctg t
//
LOCUS       DAOSCC010000293         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30867_length_2288_cov_4.150022, whole genome shotgun sequence.
ACCESSION   DAOSCC010000293 DAOSCC010000000
VERSION     DAOSCC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30867_length_2288_cov_4.150022"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLG72_11210"
     CDS             <1..532
                     /locus_tag="PLG72_11210"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ81405.1"
                     /translation="VFRLLGISSLIVTNAAGGINTSFNPGDLMLITDHISFFAPSPLR
                     GRNLDEFGPRFPDMCDAYDKNLVEYAEKIGKSIGIGLKKGVYAFAQGPMYETPAEIRA
                     LRAIGADAVGMSTVPEVITARHCGMRVLGISCITNMAAGVLEQKLDHKEVMDTAKKVE
                     DIFTRLVSEIVAKWEF"
     gene            1096..1941
                     /locus_tag="PLG72_11215"
     CDS             1096..1941
                     /locus_tag="PLG72_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ81406.1"
                     /translation="MSQIIYRETDSVDKEILNMEEAAELFNVSIKTFIKLLKEEKVPA
                     RKIGREWRFSRQALIQWLSSGDSQQYSSSESDTKEFFDRVAPEWSKLRSGYYDADVID
                     KLLSSDLLSEDMVLVDLGAGDGYLARAVALKVDTVIAVDISGAMISELRKKAAREGIK
                     NIQTIVGDGCDVPLDDGSVDIVCANMFLHHIEEPITAVREMSRILKPGGKVFLADLTE
                     HGNKELMEAMYDVWPGFSRKEVGEWFEKSGFKIEQFDILDRKESGRKPGNKEVGVFIM
                     VAVRR"
     gene            2083..>2288
                     /locus_tag="PLG72_11220"
     CDS             2083..>2288
                     /locus_tag="PLG72_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81407.1"
                     /translation="MGKIIVAGSIAYDHIMEFPGYFSELIISDRIEKLNISFLVRSLK
                     KVRGGSAPNIAYNLALTGLEAEILG"
BASE COUNT          658 a          447 c          626 g          557 t
ORIGIN      
        1 ggtattcagg ctgttgggca tatccagcct gatcgtgacc aatgctgccg gcgggataaa
       61 tacatctttc aatccgggag atcttatgct gataaccgac cacatcagtt ttttcgcacc
      121 gtcgccgctg cgcggcagaa acctggatga atttgggcca aggttccccg atatgtgtga
      181 tgcatacgat aaaaatctgg tcgagtatgc cgagaagatc ggcaaaagca tcgggatcgg
      241 actgaaaaaa ggagtttatg cgttcgcaca gggacctatg tatgaaactc cggcagagat
      301 acgcgcgctg cgcgctatag gcgccgacgc agtgggcatg tcgacagtgc ctgaagtgat
      361 caccgcgcgg cactgcggca tgagggtgct ggggatatcc tgcattacaa acatggctgc
      421 aggcgtgctt gagcagaaac ttgaccacaa ggaagttatg gatacggcaa agaaagtgga
      481 agacatattc actcggctgg tttcagaaat cgtagcaaaa tgggagtttt agcagaccgg
      541 agttgactgg aacgtatttt ctggatccaa ggttttggcc gggatcaata tgatccgggt
      601 tttacggata aaagtctaaa agaccgatat ctattggatc ggagtttagc gaatcaatat
      661 tgaaacgaag ttttgcagtt ttctgtcaat tgtatcagga gaccggatgg gtcagtaaac
      721 gcagacaata atactgtttt taagaaccgg tattgtcgtt caaatatatt atagcttttg
      781 gtgcattatc ttttttacag tctgacgtgg tatcagtata cacgccaaca atggcgccat
      841 gctcttctca gcggttccgc cggtgaactt ttgtcatgct ttatccaaaa ctaaatgaat
      901 ataagtgtag caatcgtgaa tctgttggtg atatagttgt aattgattgt tgataaacat
      961 catattaaag gatataatag gaagtagtct cacatggaag ttaaacatct tcaagaaaat
     1021 gcagaacggg acagtatttt tatgattgcc ggagcatctt atctgtgacg ccgatttgca
     1081 tgaccggagg gcgccttgtc acagattata tacagggaga ctgatagcgt ggataaagaa
     1141 atacttaaca tggaagaagc tgcggaattg ttcaatgtca gtatcaaaac attcataaaa
     1201 ctcctgaaag aggagaaggt ccctgccagg aaaatcggca gagagtggcg gttcagcaga
     1261 caagcgctga tccagtggct gtcgtcggga gattcacagc agtattcttc cagcgaaagc
     1321 gatacgaagg agttttttga cagggtcgcg cctgaatgga gcaaactccg gagcggctat
     1381 tacgatgcag atgtgatcga taaactgctc agctccgacc ttctcagtga agacatggta
     1441 ctggtcgatc tcggagcagg cgacggctac cttgcgaggg ctgttgcgct aaaagtggat
     1501 acggtcatag ctgtagacat ttccggggcg atgataagcg aattgagaaa gaaggcggcc
     1561 agggagggca taaagaatat ccagaccatt gtgggcgatg gatgcgatgt tccgcttgat
     1621 gacggcagtg tcgatatcgt atgtgcaaac atgttcctgc accatatcga agaacccata
     1681 accgctgtca gggaaatgag caggatttta aaacccggcg ggaaggtatt ccttgctgac
     1741 ctgacggaac atggcaacaa ggaactgatg gaagcgatgt acgatgtatg gcccgggttt
     1801 tcgcggaaag aggtcggcga atggttcgaa aaaagcggat ttaagataga gcagtttgat
     1861 atacttgacc ggaaggaatc cggaagaaag cccggaaata aagaagtagg cgttttcatt
     1921 atggtcgcgg taagacggtg agaacgtcct ggtgccgtac caggcgccat gatgttccag
     1981 gcagcggttg aattacggaa acatgcaaca gatgaacgga actatgcaag tgcatataga
     2041 accgtacaaa tatgcctata attcgattca aaggagatga gtatgggtaa aatcatcgtt
     2101 gccggttcga tcgcatatga tcacatcatg gagtttcccg ggtatttcag tgaactgata
     2161 atatctgaca ggatagaaaa gctgaatatc agtttccttg ttagaagttt gaaaaaggta
     2221 cgcggcgggt cagctccaaa tattgcgtac aacctggcgc ttaccggcct ggaagcagag
     2281 atactcgg
//
LOCUS       DAOSCC010000294         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30892_length_2286_cov_4.440610, whole genome shotgun sequence.
ACCESSION   DAOSCC010000294 DAOSCC010000000
VERSION     DAOSCC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30892_length_2286_cov_4.440610"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLG72_11225"
     CDS             complement(<1..308)
                     /locus_tag="PLG72_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81408.1"
                     /translation="MNIANARNTGEHGSAAQKSIWHSMMMLAPFEGVAIDSDLFDEES
                     ILEGERDFYLFMTTLYQDMYNDPAKYFIPTKPYDEYIQSILKNGERTRSAEKEHSTDP
                     "
     gene            complement(459..1172)
                     /locus_tag="PLG72_11230"
     CDS             complement(459..1172)
                     /locus_tag="PLG72_11230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ81409.1"
                     /translation="MRIRKARTDEGPILSGIALRSKAYWGYDKNFIEVCREDLTVSEQ
                     KIIENHVYVLEDSSEIIGFYCLVAKGKTGSLDFLFIDPGFIGKGYGRLLWESIMQTAR
                     EIGLEEFSIDADLLHIEEDLQTLKKREKLITVCGGEYCGELFHHLVSLPASLDDDVDV
                     VKYFEAFWDLFHNEDPERNFKLYQDFYKTAFSGFNIPITADFLGLSVLLQSYSELKPA
                     IIEISRVFADNYLIYEEHV"
     gene            1545..>2286
                     /locus_tag="PLG72_11235"
     CDS             1545..>2286
                     /locus_tag="PLG72_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81410.1"
                     /translation="MAEMVNSMAELTNINGKIPGIPGQKNSGRGGCHAGLHSARFAAR
                     ALVVYRSLGQDALISELRSLLEYVDSDWPDLFYTTDLYCSVGNRLLRAGLGLKAYIIE
                     KILHTENPFSIAAESMGTACLAGGSAGRWQAEAHDGSKDSGAASKEPDATSKDQSASP
                     VLFGKALEEAVKSDLAKLQALAEVRSEWIRDAMLHLCVEDDAMREAVMKLPPWLGDNP
                     SWQRKADGPDGAVAPDCVSLLDRDGLPGI"
BASE COUNT          573 a          535 c          536 g          642 t
ORIGIN      
        1 tttggatctg tggaatgctc tttttcggct gaccgggttc gttccccgtt ttttaaaatg
       61 ctttggatat attcatcata gggctttgtc ggtataaaat acttagcagg atcgttgtac
      121 atatcctggt ataacgttgt catgaacagg taaaaatctc tttccccttc aagtatgctc
      181 tcttcgtcaa agagatcgct gtctatagcc acaccttcaa agggagcgag catcatcatt
      241 gaatgccata tggatttttg cgctgcactg ccgtgttctc cagtatttct tgcatttgcg
      301 atattcatac cgatccgcct ttattgatag attcaggccg cctttccgcc ttggcaaata
      361 tattaagtat acttaacggt gaggcggcac aataaaccag gaactttgaa ccattgctga
      421 tataaccatt ttacgataaa accacttatc agcataggtc aaacatgctc ttcgtaaata
      481 aggtaattat cggcaaatac tcttgaaatt tcgatgattg ccggtttcag ttccgaatat
      541 gattgcagca acacactgag gccgagaaaa tccgcggtga tcgggatatt gaatccggag
      601 aatgccgttt tgtagaagtc ctggtataac ttgaaattcc gttccgggtc ttcattatgg
      661 aataagtccc aaaatgcttc gaaatacttg acgacatcga catcatcatc aagtgaagcc
      721 ggcaatgaaa ccagatgatg aaacagttcc ccgcaatatt caccgccgca cactgtgata
      781 agtttttccc gttttttcag agtctgcagg tcttcttcga tgtgcaggag atcggcgtcg
      841 atcgaaaatt cttccaaacc aatctccctg gctgtctgca tgatgctttc ccataataac
      901 ctgccatatc ctttgccgat gaaaccagga tctatgaata aaaaatccag gctgccggtt
      961 ttgcctttcg caaccaggca gtaaaacccg attatctctg atgaatcttc cagaacataa
     1021 acatgattct ctattatttt ctgctcggaa accgtcaggt cttccctgca tacttcaata
     1081 aaatttttgt cgtatcccca gtaagctttt gaacgcaaag cgattccgct caatattggc
     1141 ccttcgtctg tccgtgcttt tcttattctc attttttgca ctttatgctc cctgcctcct
     1201 tgcgcttggt cacaaccatc agatgagggg taagtttttt gatctcgagg ttcacaggct
     1261 ttcctccgta attttccata ttatgcacta atgataccat atatgcactg caaatccaac
     1321 ttatattaca aggtcatgac tagaaacggc cggaactaat tatgtgatta tttcaacttt
     1381 ccttttgcct ttcaggcgat ttgcataccc cagttccggc tggaatactt accgaatttt
     1441 ttattcaagt gtccggtcat tgccggaggg cctgaaacag tggacatctg cgctcatatt
     1501 ctgtatattt atattgaaac taatgatgac aggtggttat ctgcatggct gaaatggtta
     1561 acagcatggc tgaattaaca aatatcaatg gaaaaatccc tgggattccc ggacagaaga
     1621 atagcggccg gggaggctgc catgccgggc tgcacagtgc caggttcgcc gccagggctc
     1681 ttgtggtata ccggtccctg ggccaggacg cactgataag cgaactgcgt tctttacttg
     1741 aatatgtgga ttcagactgg cccgacctgt tctacaccac ggatttgtat tgttcggtcg
     1801 gaaacaggct gctcagggca ggccttggtt tgaaggccta catcatagaa aagatcctgc
     1861 atacggagaa tccgttcagt attgcggcag agtcgatggg caccgcatgc cttgccggtg
     1921 gaagtgccgg ccggtggcaa gcagaagcgc atgatggttc aaaagattcc ggggcggcat
     1981 caaaggagcc tgatgccaca tcaaaagacc agtcagcatc gcctgtactt ttcgggaaag
     2041 cgcttgagga ggctgtaaag agcgacctgg ctaaactgca ggcgcttgcg gaggtgcgtt
     2101 ccgagtggat aagggacgcc atgctgcatc tgtgtgtaga agatgatgcg atgcgggaag
     2161 cggtcatgaa actgccgccg tggctcggcg acaatccctc gtggcagcga aaggctgacg
     2221 gcccggatgg agccgttgcg ccggactgcg taagtttgct ggatcgggat ggtttgcccg
     2281 gaatac
//
LOCUS       DAOSCC010000295         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_30986_length_2281_cov_4.035490, whole genome shotgun sequence.
ACCESSION   DAOSCC010000295 DAOSCC010000000
VERSION     DAOSCC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30986_length_2281_cov_4.035490"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="PLG72_11240"
     CDS             complement(<1..1752)
                     /locus_tag="PLG72_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOJ81411.1"
                     /translation="MDRFSQAVISHRKSMLAVFLAAAVLGGLLSLFVSVNFNTVDYLP
                     PDAQSTTAIRIMKEEFGGEMPNARVMLTNVSIHEALEYKAKIAAVEGVADVTWLDDVV
                     GLDTLKTTPVEFLDASVAESYYRDNNALISLTVESGMEQDAVGAIYEIIGEENAAAGE
                     AVSMAESQAMSVSEVANAMALLIPIVLVILIVSTTSWIEPLFFLASIGVAILINMGTN
                     ILLGEISFITRTVSPVLQLAVSLDYTIFLLHSFNSHRQTVEPEKAMKLAMKESLSTVA
                     ASAATTMIGFAALIFMRFGIGADLGLNLLKGIIFSFISVMVFLPAITLTSYKLIDKTR
                     HKKLLPDLKKAGSVLMKIRIPFLVLAVIIAVPCFLAQSETSFLYGTSNITKATRVGRD
                     TDYIEEKFGKQNTLALLVPKGDIGTEAELSDALSRLPNVTGVISYATAVGAQIPAEFV
                     PEEALEQFYSEHYSRIIINADTDDEGAEAFDTVRSVLDTASVHYGEYWLAGQSASLYD
                     MKDIVSADNRRVNLAAVIGIFLVLLVTFRSLSLPFLLVFTIETAIWINLSFPYFAGKP
                     LNFVGYLIISTVQLGATV"
     gene            2015..>2281
                     /locus_tag="PLG72_11245"
     CDS             2015..>2281
                     /locus_tag="PLG72_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ81412.1"
                     /translation="MKKLVVGILAHVDAGKTTLSEGLLYLSGRTRKLGRVDRKDAYLD
                     NYELERARGITIFSKQAVFETGDVQVTLLDTPGHVDFSTEMERTL"
BASE COUNT          549 a          563 c          616 g          553 t
ORIGIN      
        1 cacggtcgcc ccaagctgca cggtgctgat gatcaggtaa ccgacgaagt tcagaggttt
       61 cccggcgaaa tacgggaagg agaggttgat ccatatcgcc gtttctatgg tgaaaaccag
      121 caggaaaggc aggctgagcg aacggaacgt caccagcagc acaaggaata tgcctatgac
      181 tgccgccagg ttgacacgcc ggttatctgc cgaaacgata tctttcatat cgtacaggga
      241 cgcgctctgg cctgcgagcc agtattcgcc gtagtgtacc gatgccgtat ccagcacgct
      301 gcggacggta tcgaatgcct cggcgccttc gtcatccgta tcggcgttta tgattatcct
      361 tgagtagtgc tcggaataaa actgctccaa tgcctcctcg gggacaaatt ccgccggtat
      421 ctgtgctcct acggctgttg catacgatat cacgcccgtt acattgggca gcctggaaag
      481 agcgtctgac agttcagctt cggtcccgat atctcctttt ggcacgagaa gtgccaacgt
      541 gttctgcttt ccgaacttct cttcgatata gtcggtatcg cgccccactc tcgtggcttt
      601 ggttatattg ctggtaccat acaggaaact tgtttccgac tgggccagga agcagggtac
      661 cgctatgatg acggcaagga ccaggaacgg tatcctgatc ttcatcagga cactgcctgc
      721 ttttttgaga tccggcagca gctttttatg ccttgtttta tcaataagct tgtatgaggt
      781 cagtgtgatc gccggcagga ataccatgac cgaaatgaag ctgaatatga tgcctttcag
      841 caggttgagg cccaggtccg cgcctatccc gaagcgcatg aatatgagcg cggcgaagcc
      901 gatcatcgtg gtggcggcgc tggctgccac cgtcgagagc gactccttca tggccaactt
      961 catagccttt tcgggttcaa cggtctgcct gtggctgttg aagctgtgga gcaggaaaat
     1021 agtgtagtcc agcgacacgg caagctgcag gaccgggctg accgtcctgg taataaacga
     1081 tatttcgccg agaagtatgt tggtccccat gttgatcagt atcgcaacgc ctatggacgc
     1141 caggaagaac agcggctcta tccatgaggt ggtggagacg atcaggatga cgagtacgat
     1201 cgggatcaga agcgccattg cgttggctac ttctgaaaca gacatcgcct gcgactctgc
     1261 catggaaacg gcttcgcccg cagcggcgtt ttcttcgccg attatctcat aaatagcccc
     1321 gactgcgtcc tgctccatac cggactccac agtcaggctg atcagcgcgt tattgtccct
     1381 gtaatagctt tcggctacgg atgcgtcaag aaattcaacg ggagtcgttt tcagcgtgtc
     1441 gaggccgaca acgtcgtcga gccaggtcac atcggccacg ccttccactg cggcgatttt
     1501 cgccttgtac tccagcgctt catgtatcga tacattcgtg agcatcactc ttgcattcgg
     1561 catctcgccg ccaaactctt ccttcattat cctgatcgca gtcgtggact gtgcgtcggg
     1621 cggcagataa tccactgtgt tgaaattgac ggaaacaaac agcgaaagca gtccgcccag
     1681 tactgcagcg gccaggaaca cggcaagcat gctttttcta tggctgataa cagcctgtga
     1741 aaacctgtcc atgaaattca cctcttttgc tatacacggt gtcgataaat aactctatgt
     1801 atagtttata tacactttgg cgtaaattca acaaacagat gaataagata tgtatgttga
     1861 taatcacatg ggtctaaagt gtttgttatc agataaatta ttcaaaactg acgggtatcg
     1921 gacagacatg gggtgtttgt gtgaacaggg ttggtggtaa gttgctgaat acggtataat
     1981 gttaatacaa tgggaggatg gaagatccgc acatatgaag aaacttgtcg ttgggatact
     2041 ggcgcatgtt gacgcgggaa aaacaacatt atcggagggc ctgctttatc tgagcggcag
     2101 gaccaggaag ctcggaagag tggacaggaa ggacgcctac ctggacaact acgaactgga
     2161 aagggccagg gggatcacca ttttttccaa gcaggctgtg tttgaaacag gggatgtgca
     2221 ggtaacattg ctggataccc ccgggcatgt ggacttttcg actgaaatgg aaaggactct
     2281 c
//
LOCUS       DAOSCC010000296         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_32076_length_2225_cov_4.601843, whole genome shotgun sequence.
ACCESSION   DAOSCC010000296 DAOSCC010000000
VERSION     DAOSCC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32076_length_2225_cov_4.601843"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PLG72_11250"
     CDS             complement(<1..1496)
                     /locus_tag="PLG72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOJ81413.1"
                     /translation="MRDDLEFYPLTQPQLGIWYTEKMFPDTSIGIIAGTLKLKGHIDY
                     PLLKRAVSIYIENNDTLRLRFREVDGEPRQYYLETPLKEPEVYDFSSSGKEALYKWDT
                     DRTRTPFKVIDSDLYEFCIIRLSDQEAGVFMKFHHLICDAWSIVNAGNDICRIYSDLL
                     DGSYTGFEPLPSYRAFIEDESRYINSDKFEKDRSFWKETYSSYPEVSALKIRKTNTPS
                     SAARRRSFVLPDRLCKKITEFCNENRTSVFMLFFCALSIYINRVLNKEDIIIGVPVLN
                     RSNAVHKKIMGMFISTVPVRIYIDTGLSFADFLDNATKEWMKALKHHKYSYNMIISDV
                     REKFAGITDLYDITLSYQNAKLNKDCRVYNEGRWHFCGHQKQSLVIHVNDRENDGSIV
                     VDYDYLEDIFAEKEIEFLHDHMIRILWHGLDNPRRNIAKLEMVSEREKKKIVYEFNKT
                     ACDYPAGKTIIRLFEEQVGRTPYKPAVYYADRSMTYDEFNRKVNRLAW"
     gene            complement(1630..>2225)
                     /locus_tag="PLG72_11255"
     CDS             complement(1630..>2225)
                     /locus_tag="PLG72_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOJ81414.1"
                     /translation="AAKLQDEKEMRRAAEEAKAKLRNSIGKIEESLTDGLFQRKGFVK
                     PPENLKPGDSVLILDLNKKGTVITPPDKDGEALVQAGIMKINVHVTNLKLIDEQSYEI
                     QRTGSASIGVSKAMNISPRTDIRGMNVEEAIIILGKYLDDAALAGLSEVTIVHGKGTG
                     ALRSGVHQYLKTNHHVKSFRLGQYGEGEAGVTIVTLK"
BASE COUNT          507 a          564 c          477 g          677 t
ORIGIN      
        1 ttccatgcaa gtcggttcac cttcctgttg aactcgtcat aagtcatcga cctgtcagcg
       61 taatacaccg cgggcttata cggcgtcctt ccgacctgct cttcgaacag cctgataatg
      121 gtctttccgg caggataatc acatgctgtt ttgttgaact cgtaaactat cttctttttc
      181 tccctttccg acaccatctc cagcttcgct atgtttctgc gcggattatc aagcccgtgc
      241 cacaggatcc tgatcatgtg gtcatggaga aactcgatct ctttttcagc gaaaatatcc
      301 tctaggtaat cataatcaac aacaatagaa ccgtcatttt cacggtcatt tacatggatg
      361 acgagggact gcttctggtg tccgcagaaa tgccatctgc cttcattgta cactctgcag
      421 tccttgttca gcttcgcgtt ctgataggac agggtgatat cgtacaggtc cgttatcccc
      481 gcgaactttt cgcgcacatc gctgatgatc atgttatatg aatacttgtg gtgtttgagc
      541 gccttcatcc attccttcgt ggcattgtca agaaaatctg caaaactcag gcctgtatcg
      601 atataaatcc ttactggcac tgtactgatg aacatcccca ttattttctt atgaacggcg
      661 ttcgaccggt tcagcacggg gaccccgatt ataatatctt ctttatttag taccctgttt
      721 atatagatgg aaagggcgca aaaaaacagc atgaatactg atgtccggtt ttcattgcag
      781 aactccgtta tctttttgca cagcctgtcc ggcaatacaa aagatcttct ccttgccgca
      841 gatgatggag tattcgtttt gcggatcttc agggcgctca cctcgggata gctgctgtat
      901 gtctctttcc agaatgaccg gtccttttcg aatttatctg aattaatata cctgctttcg
      961 tcttctataa aagccctgta ggatggaaga ggttcgaagc cggtgtaact tccgtcaagc
     1021 aagtctgaat atatcctgca tatatcgttg ccggcattca ctatggacca cgcgtcacag
     1081 ataaggtggt ggaatttcat gaatacgcct gcttcctggt cactgagcct tattatgcag
     1141 aattcataga gatcggagtc gatcactttg aatggagtcc tggtcctgtc cgtgtcccat
     1201 ttgtaaagcg cttctttccc tgaagacgag aaatcatata cctcgggttc tttcagcgga
     1261 gtctccaaat agtactggcg tggttctccg tcaacctccc tgaagcggag ccgcagcgtg
     1321 tcattatttt caatgtatat cgaaacggct ctcttcagca gcggatagtc gatatggccc
     1381 tttaacttca acgtgcctgc gattatgcct atactggtat ccgggaacat cttctccgta
     1441 taccatatac ccaactgtgg ctgagtcagg gggtaaaact ccagatcgtc tctcaatgcg
     1501 gacacctcga agaaattagt ttttatgcca tattatggca tatgctccat catcagcatt
     1561 atattgtatt ttctgactaa attcaagaca aagtgtgaaa tttgttaaga tttgcacaaa
     1621 ttttgcatat tatttcagcg taacgatggt gacaccggct tcaccctcgc catactggcc
     1681 aagccggaaa cttttgacat gatggttggt cttcagatac tgatgaacgc cgctcctgag
     1741 cgcgcctgtg cctttcccgt gtacgatcgt tacttcggaa aggccggcaa gtgccgcatc
     1801 gtcgaggtat ttgccaagga tgatgattgc ttcttccacg ttcatgcccc tgatatccgt
     1861 acggggcgat atattcatag ccttggacac accgatggat gcggaaccgg tcctctggat
     1921 ctcgtaagac tgttcatcga tcagtttgag atttgtgaca tggacattta ttttcattat
     1981 accggcctgg acaagagctt ccccgtcctt gtccggcggt gttatcacgg tccccttctt
     2041 gttaaggtca agtatcagaa cactatcgcc gggcttaaga ttttccggcg gtttcacaaa
     2101 gcccttccgc tggaaaaggc catcagtcag cgactcttcg atcttcccga tactgttcct
     2161 gagctttgct tttgcttctt ccgccgctct tctcatctct ttctcatcct gcagttttgc
     2221 cgcct
//
LOCUS       DAOSCC010000297         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_32199_length_2220_cov_3.013857, whole genome shotgun sequence.
ACCESSION   DAOSCC010000297 DAOSCC010000000
VERSION     DAOSCC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32199_length_2220_cov_3.013857"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..1851
                     /locus_tag="PLG72_11260"
     CDS             571..1851
                     /locus_tag="PLG72_11260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ81415.1"
                     /translation="MKKIRILTIAALIILIAAGVTIAILYHSSAYRDRQVLHEQPYEE
                     IPNGNNGEGNVTGNDNGYEPVSEEGNGQEPGEGQGDPSADGNVQPDDGAGNAEEPDIP
                     ELRFIAVGDIMPGRGVEYWIKQKGGGYESAFAKVKPLLEQGDIVFGNLECPLTSSSKG
                     LYKTGKIVLKASPESVTALTSAGFNLLSLSNNHIMDYYERGLFDTMELLDQHGIVHAG
                     GGRNIDEAREMAVIEKNGLKIGLLAYTDMAEIVFAGDPYLSFAAGPEKSGVAPRKYET
                     VREDIGKNRDKVDLLVVSLHWGIEDSFRVTEEQVEFAHKLIDDGADLILGHHPHQFQG
                     IEIYNGKPILYSMGNFLFDQNESENMESFIVDMKFEGNELAGLSAVPVRIIKKSYVEI
                     QTGADASELLARQADLSRRLGTEPVIENDILIYK"
     gene            complement(1917..>2220)
                     /locus_tag="PLG72_11265"
     CDS             complement(1917..>2220)
                     /locus_tag="PLG72_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ81416.1"
                     /translation="IGFIAGAYVIGRISAKFEKPTPVNNLKALLPGLISIYVPGIVHM
                     LLIMRIYLGNKQAGLLVVLSGNLPYFIKDVFLFVIIAFSGASLLPVVRKALYNKAS"
BASE COUNT          631 a          483 c          598 g          508 t
ORIGIN      
        1 cgtctttata acgcatcatt taaagacaac ttcccggttg cagggaattc ttcctgcttc
       61 tgcgcgtgga agatataccc tccgcctgaa aggcaaatca gcacccgccg cctgcagagg
      121 cgggggacat acattatcgc ttctttttat tatgatgtgg aatatttgcc tttatataag
      181 ttgtatattc tgtaaatttt ctgtttatca gcgattcagt gtggtattta atatctgatc
      241 cgcaatagtc attataatcc atatcaaggt ctgcatgcaa tatagtacag aaatgaggga
      301 ggttttggaa ggcaatcatg aaaagcctga aggaacaaaa acgcttgaaa acctcatgaa
      361 atcatttcca gttgttcgtc gaaggatatt gtcgatttgt ataaatggct gacaacacgg
      421 taccggacag cggactgctg cccggaccga tccgatattc gaccccttat tgcctgctgt
      481 atgcttaaag catacagctc tttttataat caggtgacag attgcataat tagtgttaat
      541 atatcctaag aaccgattca ggagaggatt atgaagaaga tcaggattct aaccattgct
      601 gcacttataa tactgattgc cgcgggcgtg accatagcga tcctttacca cagcagtgcg
      661 tacagggaca ggcaggtgct gcatgagcag ccatatgaag agattccgaa tgggaacaac
      721 ggcgaaggaa atgtaactgg aaatgataat gggtatgagc cggtgtctga agagggaaac
      781 ggacaggaac cgggtgaagg gcagggagat ccttcggcag acggcaacgt gcaacctgat
      841 gacggagccg gaaacgctga agaaccagat atacccgagt taagatttat tgcggtaggc
      901 gacataatgc ccggcagggg agtcgagtat tggattaagc agaaaggcgg aggttacgaa
      961 tcagcgtttg cgaaggtaaa acctctgctg gagcagggcg acatcgtgtt cggaaatctc
     1021 gagtgccccc ttacatcaag cagcaaaggc ctttacaaga cggggaaaat agtgctcaaa
     1081 gcctcgcctg aatcagtcac ggcgctgacc agcgcaggct tcaacctgct gagcctgtca
     1141 aacaaccata tcatggatta ttatgaaaga ggcctgttcg acacaatgga actgctggac
     1201 cagcacggca tagttcacgc aggcggcggc aggaatattg atgaggcaag agaaatggcc
     1261 gttatagaga aaaacggcct taaaatcggg cttctggcat acaccgacat ggctgagatc
     1321 gtttttgcag gcgatcctta cctgtcattt gcggcaggtc ctgaaaagtc cggcgttgcg
     1381 ccgagaaagt atgagaccgt cagggaagat atcgggaaga accgtgacaa ggtcgatttg
     1441 ctggttgttt cactgcactg gggaatagag gacagtttca gggtgaccga agagcaggtg
     1501 gagtttgccc acaagctgat agatgacggc gccgacctga tcctggggca ccatccgcac
     1561 cagttccagg ggatcgagat atacaacggc aagcctatcc tgtacagcat ggggaatttc
     1621 ctcttcgacc agaacgagtc tgaaaacatg gagtcgttca tagtcgatat gaaattcgaa
     1681 ggcaacgaac tggccgggct gtcggccgtg cctgtgagga tcattaaaaa atcgtacgtg
     1741 gagatccaga cgggcgccga cgcttccgaa ctgcttgcaa ggcaggcgga tctcagccgc
     1801 aggctcggga ctgaacctgt tatcgaaaat gatatactga tatacaaata gtgccgacca
     1861 gagaagccgg caggcaatac acggagcgaa aaagtacgtt cggtttgata atgatgtcag
     1921 gaggccttgt tataaagtgc cttcctgaca accggaagca atgaggcgcc ggaaaatgcg
     1981 attatgacaa acaggaagac atcctttata aaatacggca ggtttccgga gagtaccacc
     2041 aggaggccag cctgcttatt tcccaggtaa atgcgcatta tcagcagcat atggactatt
     2101 ccggggacgt atatggagat aaggcccggc agcaatgctt tgaggttgtt tacaggcgtc
     2161 ggtttttcaa actttgcgga tatcctgccg ataacataag ctcctgctat gaaaccgatg
//
LOCUS       DAOSCC010000298         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_32593_length_2201_cov_4.886300, whole genome shotgun sequence.
ACCESSION   DAOSCC010000298 DAOSCC010000000
VERSION     DAOSCC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32593_length_2201_cov_4.886300"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLG72_11270"
     CDS             complement(<1..609)
                     /locus_tag="PLG72_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOJ81417.1"
                     /translation="MNVIKGGVTAPEGFFATGVACGLKKDGRKDLAIVCSEDSAAIAG
                     VYTTNKVKGHSLQLTMDHAKTGCANAIVINSGNANACVGKQGYEDALKIAEATSELLD
                     CRPQDILVASTGVIGVPLDMDAIIPALDELVSNMDPDGGHDAAEAIMTTDTVPKEIAI
                     ELEHVAEVVGTGKAEAAVDLRRDVVVAHLASERAGEGGGHRRA"
     gene            1077..1382
                     /locus_tag="PLG72_11275"
     CDS             1077..1382
                     /locus_tag="PLG72_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81418.1"
                     /translation="MALRPIMRMKGFDFEREWHMLVLEILGGLFMAAGALMVAAAKKI
                     VKRYGLDKKVVLEHESELDETETEEYKTLKATVNIKLYGMLVFLPGLVFLLIAFNKA"
     gene            1499..>2201
                     /locus_tag="PLG72_11280"
     CDS             1499..>2201
                     /locus_tag="PLG72_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ81419.1"
                     /translation="MERINELAKKYDPSQVEERLYREWTEKGYFHAEPDDSKTPFTIV
                     IPPPNITGQLHMGHALDNTLQDILIRWKRMQGYCALWLPGTDHASIATEAKIVETMAK
                     EGLKKEDIGREKFLERAWEWKKVYGGRIVEQLKKLGSSCDWERERFTMDEGLSKAVLE
                     VFVRLYEKGLIYRGDRIINWCPKCCTSISDAEVEYEEQEGSFWHIKYPIKDSDEFVTV
                     ATTRPETMLGDTAVAV"
BASE COUNT          569 a          534 c          623 g          475 t
ORIGIN      
        1 tgcgcggcga tggccgccgc cttcacccgc gcgctcggac gcgaggtgcg ctacaacgac
       61 gtcgcgccgg aggtctaccg cggcttcggc tttcccggtg ccgacgacct cggcaacatg
      121 ttccagttct atcgcgattt ccttgggaac ggtatcggtg gtcatgatag cttccgcggc
      181 gtcatgtccg ccgtcaggat ccatgtttga gacgagttcg tcaagggcgg gaataatggc
      241 atccatgtcg aggggcactc cgatgacacc tgtggatgcc accaggatat cctggggcct
      301 gcagtcaagc agttcggatg tggcctcagc gatcttcagc gcgtcctcgt aaccctgttt
      361 gccgacgcag gcattggcgt tgccgctgtt gatgacgatc gcgttggcgc aacccgtttt
      421 tgcatggtcc atcgtcagct gaagcgaatg ccctttgacc ttattcgttg tataaacgcc
      481 ggctatagcg gcgctgtctt cagaacatac gatggcaaga tccttcctgc cgtccttttt
      541 aagtccgcat gccacacctg ttgcaaaaaa tccttccgga gctgtcactc cgccttttat
      601 aacattcatc gaaacactcc actcatgtac agaatctttt ctaccttttg ctttaaacaa
      661 gtaaattata cataatatac gctgagggtt caataaaaac cgtccgaaat tgccggatat
      721 ggatctgcga tcagttttca tttttcctgg gttttctgtg cagcttttgg gcatgctggc
      781 agtacccatg cgccgggtgc ttctcccctt gacacttttt tcgtaatata ttatattaac
      841 caatataaat tgaaaaagta caagacgttg atcgggagga gtaagcagga cctgccccac
      901 aggtttgcaa gagagaaaga cccgcaggct ggaaggtctt tcggggataa ggctgctgaa
      961 ggtcgcccgg gagtcctgca gcataaagcc gccggacggc ggaacaactg cagcggcaaa
     1021 gggaccgtta ttcctgtgag agccctgtaa agggaatttt ggtggtaccg cggagcatgg
     1081 cccttcgtcc aataatgagg atgaagggct ttgattttga aagggagtgg catatgctgg
     1141 tactcgagat attgggcggg ctctttatgg ctgccggcgc cctgatggtg gcagcggcga
     1201 aaaagatcgt gaagagatac ggccttgata aaaaagtggt acttgagcat gagtccgagt
     1261 tggacgaaac agagaccgaa gaatacaaaa cgctgaaggc aacggtaaat atcaagcttt
     1321 acggcatgct cgtcttcctg cccgggctgg tgttcctgct gatagctttc aataaggcat
     1381 aggatacggc agggcaactg ccggacaaat catggacaag tcttgcggct gatgaagacg
     1441 gacagcccga atcatatgat ataaaaatcg aaatcaactg cagccaggag gagatcaaat
     1501 ggaaaggata aatgaattgg caaaaaaata cgacccgtca caggtggaag aaaggctgta
     1561 cagggaatgg acggaaaagg gatatttcca tgcggagccc gacgacagca agacgccgtt
     1621 cacaatagtg atacccccgc cgaacatcac cggccagctt cacatggggc acgccctcga
     1681 caatacactg caggacatac tgatcagatg gaagagaatg cagggctatt gtgcgctttg
     1741 gctgccgggc acggaccatg ccagcatagc caccgaggcc aaaattgttg agacgatggc
     1801 aaaagaaggc ctcaaaaagg aagacatagg cagggagaaa ttccttgaga gggcctggga
     1861 atggaaaaag gtctacggcg gcaggatagt tgagcagctc aagaaactcg ggagttcatg
     1921 cgactgggaa agggaaaggt tcaccatgga cgaaggcctt tcgaaggccg tactggaggt
     1981 attcgtaaga ctttatgaga aaggcctcat ttacaggggc gaccgcataa tcaactggtg
     2041 ccccaagtgc tgcacgtcca tatccgatgc ggaggtcgag tacgaggaac aggaaggcag
     2101 cttctggcat ataaagtatc cgataaagga cagcgacgag ttcgtgacgg tagccaccac
     2161 caggcctgag acgatgctcg gcgacacggc agtcgccgtc a
//
LOCUS       DAOSCC010000299         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_32632_length_2199_cov_4.162313, whole genome shotgun sequence.
ACCESSION   DAOSCC010000299 DAOSCC010000000
VERSION     DAOSCC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32632_length_2199_cov_4.162313"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLG72_11285"
     CDS             <1..252
                     /locus_tag="PLG72_11285"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOJ81420.1"
                     /translation="VFPFEWVTVNYYEVATMEAHISEEDARETAAREAYEKALGRVPE
                     EAEIVKENVYFTEQDGKLTARAVLECIENIGIPSRIGGN"
     gene            253..1218
                     /locus_tag="PLG72_11290"
     CDS             253..1218
                     /locus_tag="PLG72_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOJ81421.1"
                     /translation="MDGFEKTLELQRVEQAISLFGNFDENVRIIEDGLNVRMITGDKG
                     IRITGDTENVEKAYAVLEKMVSIIEQGEELTRQNVRYLVDLAADGMIDKITGYSDDHI
                     CVTARGRHIKSKTHGQKKYVKAIRDNTVVFGIGPAGTGKTFLAVAMAVNAFKDKQVSR
                     IVLTRPAVEAGEKLGFLPGDMQNKVDPYLRPLYDALYEIMGVESYHKYLEKGMIEVAP
                     LAYMRGRTLDDSFIILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDITQIDLPRDKKSG
                     LKEVTKILKNIEGIEFVYLTEKDVVRHELVQRIIKAYDKYDQNKD"
     gene            1233..>2199
                     /locus_tag="PLG72_11295"
     CDS             1233..>2199
                     /locus_tag="PLG72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOJ81422.1"
                     /translation="MKAVNKKKSRRRVLSGLKNKAIQRFIVGLITVTIAAVIVENGAA
                     PKKYRLEVGESSKYDITAPRDIVNVLLTEKLAAEAAANVPPVMVRLDDVPIDIINSLN
                     EFIDYIESARQKINGLYDRGSDTVDGGIADMTEQETAAADYLILKLDELGVKITADQA
                     GFLLKTATDEEMGKFETLLISTVSEVMKSEVNADNLAEKTLYIQNIFQISDLPQELKD
                     LGGFTAKALLRPNCEIDEEATRIARDEAYEAALENKQIIPEGSRIISFGDIVTEDKLE
                     VLRELNLLETEGFDYRFAAGILAVVLMLSFLLIWYIRVFCSKVMPG"
BASE COUNT          667 a          446 c          635 g          451 t
ORIGIN      
        1 gtctttccct ttgaatgggt cactgtgaac tattatgaag tggcaacgat ggaagcccat
       61 atcagtgaag aagacgccag ggaaacggca gcaagggaag cgtacgagaa ggcgcttgga
      121 cgcgtacctg aagaggctga aatagtgaaa gagaacgtat acttcactga gcaggacggg
      181 aaactgaccg caagagccgt tctggaatgc attgaaaata taggtatacc cagtcggatt
      241 ggaggaaatt gattggacgg ctttgaaaag acactggagc ttcaaagagt ggaacaggca
      301 atcagcctgt tcggcaattt tgacgaaaat gtgcggataa tcgaagacgg tctgaatgta
      361 aggatgatta caggtgacaa agggatcagg ataaccggag acacagagaa tgttgaaaag
      421 gcttatgcgg tccttgagaa gatggtttca atcatcgaac agggagaaga actgacaagg
      481 cagaatgtgc ggtatcttgt agatcttgct gcagacggca tgatcgacaa gataaccggg
      541 tattcggacg atcatatatg tgtgacggcc agaggcaggc atatcaagtc caagacccat
      601 gggcagaaaa aatatgtaaa ggcgatcagg gacaatactg tagtgttcgg gataggtccg
      661 gcaggcacag gcaagacgtt ccttgcggtg gccatggcgg tcaatgcatt caaggacaag
      721 caggtaagca ggatagttct gacaaggccg gcggtcgaag ccggggaaaa gctgggcttt
      781 ttgcccggtg acatgcaaaa caaggtagat ccctatctga ggccgcttta tgacgccctg
      841 tatgagataa tgggagtcga atcataccat aaatacctgg aaaaaggcat gatagaagtg
      901 gcgccgctgg catacatgag agggcgtacg ctggatgatt cgttcataat ccttgatgaa
      961 gcgcagaaca ctacgcccga gcagatgaaa atgttcctta cgcgcattgg attcggatcg
     1021 aaagcggtgg tcaccgggga cataacccag atagaccttc cccgggacaa gaaatcaggg
     1081 ctgaaagaag tcacgaaaat actgaagaat atcgaaggta tagagttcgt atatctgact
     1141 gaaaaggacg tcgtcaggca tgagcttgta caaaggatca taaaggcata tgataagtat
     1201 gatcagaaca aggactgatt gggggcttcg tattgaaggc tgtcaataaa aagaaatccc
     1261 gcagaagagt gctgtccggt ctgaaaaaca aagctatcca gcgtttcatc gtcgggctca
     1321 taacagtgac gatcgcggcc gtcattgtcg agaacggagc ggcgccgaaa aagtacaggc
     1381 ttgaagtggg cgaaagctca aaatatgata tcacggcgcc gagggacatt gttaatgtgc
     1441 tgctgactga aaagctggca gcggaagccg ccgcgaacgt gccgccggta atggtgaggc
     1501 ttgacgatgt cccgatagac ataatcaact cacttaatga gtttattgat tatatagagt
     1561 cggcaaggca aaaaataaac ggcctttatg accgtggttc cgataccgtc gacggaggca
     1621 ttgccgatat gacagagcag gagactgctg cggccgatta cctcatactg aagctggatg
     1681 agcttggagt aaagataacc gccgatcagg ccggtttcct gctgaaaacg gcaactgacg
     1741 aagaaatggg caaattcgag actttgctca taagcacggt cagcgaagtg atgaaatcag
     1801 aagtcaatgc cgacaacctg gccgaaaaga cgttatatat acagaatatt tttcagatca
     1861 gcgaccttcc ccaggaactg aaggatttgg gcggattcac ggcaaaggct ctgctcaggc
     1921 cgaactgcga gatcgacgag gaggctacga ggatagccag ggatgaagcg tacgaggcag
     1981 ccctggaaaa taaacagatc ataccggaag gctcaaggat cataagtttc ggcgacatcg
     2041 tgaccgaaga caagctggaa gtcctcaggg aactcaacct ccttgaaacg gaagggttcg
     2101 attacaggtt cgcggcaggg atactggctg tcgtgcttat gctgtcattc cttctgatct
     2161 ggtatataag ggtcttctgc agcaaggtaa tgccgggta
//
LOCUS       DAOSCC010000300         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_32759_length_2193_cov_2.677736, whole genome shotgun sequence.
ACCESSION   DAOSCC010000300 DAOSCC010000000
VERSION     DAOSCC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32759_length_2193_cov_2.677736"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLG72_11300"
     CDS             <1..425
                     /locus_tag="PLG72_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81423.1"
                     /translation="FNDFIVKYAKPTVRMGGFSRNDWSCVYSLNKSNRVIMSLNIVQE
                     NLSVKLNLANISRYADSLEQYPEEIRHAIMTCGWDCGHCRGNCAGPFSFTYEGKDYNK
                     CRCGSFVFGHINKDTARYCIDLLEKEIRAEKDGLAPDQ"
     gene            613..1092
                     /locus_tag="PLG72_11305"
     CDS             613..1092
                     /locus_tag="PLG72_11305"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOJ81424.1"
                     /translation="MTSRTFGNLMRISVIAVALSGLFLCAYVIPSWGQSIVNAYPGLG
                     GWYRPWLVFEWLVAIPCFAVLVYVWKVSDAVMKDTVFTMLTAKWVKMGSVLLLLDAAL
                     LFVGNVILLLAGMSHPGILLMSVIGDIFIVGLALLAAVLSRYLTKAAILQEESEGTI"
     gene            1089..1322
                     /locus_tag="PLG72_11310"
     CDS             1089..1322
                     /locus_tag="PLG72_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ81425.1"
                     /translation="MIIINVDVMLAKRKMSVTELAERVGFTMANVSMIKTGKVKALKL
                     STLDKLCEALDCQPGDILEYRKDANGDERNGDE"
     gene            1300..1662
                     /locus_tag="PLG72_11315"
     CDS             1300..1662
                     /locus_tag="PLG72_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81426.1"
                     /translation="MNETATNKTVRIIFNSIYGIGAAIVIALCGIALFGSDQPVNPDA
                     MIPLSPKEQAFIWLVFGTIPMLLACMAVYKFNAIKNSTNRKRNFALIFLPGFICSACA
                     LYMVALVIYEIIDYYLLR"
     gene            complement(1705..>2193)
                     /locus_tag="PLG72_11320"
     CDS             complement(1705..>2193)
                     /locus_tag="PLG72_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ81427.1"
                     /translation="AAAAALSSGVYCELITDMVHVHPGLFEMIRKAKGVDRITLITDC
                     MEAGGMPDGNYTLGGRPVTVKGNEARLADGTLAGSVLRLNRGLYNFYKHTGAELHEVF
                     RMASANPAKELGIDDRKGSIKPGKDADFILIDENFDVKATYVKGVCVWRTVPHHHPHH
                     HL"
BASE COUNT          543 a          501 c          559 g          590 t
ORIGIN      
        1 ttttcaacga tttcatcgta aaatacgcaa agccgacggt gaggatggga ggattcagcc
       61 gcaacgactg gtcgtgtgta tatagtctca ataaaagcaa tagagtcatc atgtcattga
      121 acatagtcca ggaaaacctc tctgtaaagc tgaacctggc aaatatcagc cggtacgccg
      181 acagcctgga acaataccct gaagagatca ggcacgcgat catgacctgc ggctgggatt
      241 gcggacactg ccgcggcaac tgcgcaggac cgttttcatt cacctatgaa ggaaaggatt
      301 ataacaaatg tcgttgcggt tcctttgtgt ttggtcacat caataaagat acggcccgtt
      361 actgtatcga tctcctggag aaggagatac gggctgaaaa agatggcctg gcaccggatc
      421 aatagaggtc ccgcctgttt catttggaga tttaagcccg gatcgcacgc gtgattgtat
      481 gataggatga aaactggata ttaagctgta tgttgtattg caataaaaat ttatcgtaaa
      541 acgataaatt tttattgcaa tatttattta gctgttatat actttaagca gaaaagataa
      601 ggaggtcttc acatgacatc aaggactttc gggaacctta tgcgcatttc tgtgatagct
      661 gtcgccttga gcggcctgtt cctctgtgcc tatgtaatac catcatgggg gcagagtatt
      721 gtcaatgcat atcctggatt aggcgggtgg taccggccgt ggctggtgtt tgaatggctg
      781 gtggcgatcc cgtgctttgc tgtgcttgtt tatgtatgga aagtatcgga tgcagtgatg
      841 aaagacaccg tgttcaccat gctgacggca aaatgggtca agatggggtc tgtactgctc
      901 ttgctcgatg cagcgctcct gttcgtcgga aacgttatat tgcttctggc tggtatgagc
      961 catccgggca tccttctcat gtctgttata ggggatatat ttatcgttgg cctcgccctg
     1021 ctggcggcag tcctttcgcg ctacctgaca aaagccgcca tactgcagga agaaagcgag
     1081 ggtacgatat gatcataata aacgtcgatg ttatgctcgc aaagcgcaaa atgagcgtta
     1141 ctgaacttgc agagcgcgtt ggttttacaa tggccaatgt gtcgatgatc aagacaggca
     1201 aggtcaaggc gctgaaactt tctacgctcg ataaactgtg tgaagctctt gactgccagc
     1261 ctggcgatat tttggaatac aggaaggatg caaacggcga tgaacgaaac ggcgacgaat
     1321 aaaactgtga ggataatctt caacagtatt tacggcatag gtgcggccat cgttatagct
     1381 ctttgcggta tcgcgctttt cggatcggac cagcccgtaa accccgacgc catgatccct
     1441 ctttctccga aagaacaggc gtttatatgg ctcgtatttg ggacaatacc catgctgttg
     1501 gcatgcatgg cagtatataa gttcaatgca ataaagaaca gtacaaacag gaagcggaat
     1561 tttgccctca tcttcctgcc gggctttata tgcagcgcat gtgcgctata catggttgca
     1621 ctggtgattt atgagataat agactattac ctgctgcgct gagtggtcat tgcagtccct
     1681 gagacaatat ttgccgcgtt ctgatcatag atgatgatgc ggatgatgat gcggaactgt
     1741 gcgccacaca catactcctt ttacataggt ggccttaacg tcaaagtttt cgtcgatcag
     1801 gatgaaatcc gcatctttgc ccggttttat cgaacctttc cggtcatcta taccaagctc
     1861 ctttgcggga ttggccgaag ccatcctgaa aacttcgtgg agttctgcgc cggtatgttt
     1921 atagaaattg tacaatcccc tgttcaacct gaggacgctt ccggcgaggg tcccgtcggc
     1981 cagccttgct tcgtttcctt tcactgtcac aggccggcct ccaagcgtgt agttgccgtc
     2041 aggcatgccg ccggcttcca tgcagtccgt gatgagagtt atcctgtcaa cacccttggc
     2101 tttccttatc atctcaaaga gtcccggatg gacatggacc atatctgtga tcagttcgca
     2161 gtatacgccg cttgacagtg cggcagccgc tgc
//
LOCUS       DAOSCC010000301         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_33331_length_2166_cov_3.437707, whole genome shotgun sequence.
ACCESSION   DAOSCC010000301 DAOSCC010000000
VERSION     DAOSCC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33331_length_2166_cov_3.437707"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLG72_11325"
     CDS             <1..429
                     /locus_tag="PLG72_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOJ81428.1"
                     /translation="QVNDLTQKDQENAKKLAELETAVKTAQDEAMALQETLKSAEAAR
                     DEALSKLAAAETAAMVMKAQTEGLTLEELARFNGKDGQPAYIAVDGIIYDVTESRAWK
                     DGMHNGFEAGKDLTEEIKTKSPHGIGKLEGITQVGKLKAE"
     gene            complement(462..1877)
                     /locus_tag="PLG72_11330"
     CDS             complement(462..1877)
                     /locus_tag="PLG72_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ81429.1"
                     /translation="MYPDWVLKEKRPGTNISKIGNNYYLYEVTSQWDKEKKRAKKITK
                     SYIGRITESGLIPKMERMTRIDADPPLVLHYGGALLASQVGAEILEKLREHFGPSSET
                     IFTAAALRLLHSHPFKRLEAEYSHSYLSVLFSGLKMGGKSMSSFLQRLGDDRPAIAEF
                     MKAFMSGTEHILFDGTSIISKSEKMEHNRVGYNAHRQYDPQVNLLYAFSVESNQPAYY
                     RIVAGNVRDVTAFKLCIEESGIKDMTIVADKGFGSKENFELLESAGLRYIVPLRRNSE
                     AYSHTRFESGNKADFDGHFMFNTRPIWYYSGDGICYFLDSELRAQEEKDYCLRIEKKI
                     ENYTDSSLLDKQFKFGTIALKTNLNLSPQEVYCLYKQRTNIEQTFDFLKNLMDQDKSY
                     LQSQAAMEGWAFINHISLLLCYKLYNLLRDKKLLSRFSIADLISHLNYIHKIKISDTW
                     QTSEISKKTLALLTELGITIT"
BASE COUNT          556 a          518 c          528 g          564 t
ORIGIN      
        1 caggtcaacg atctgacaca aaaagatcag gaaaacgcca agaaactggc agagcttgaa
       61 acggcagtga aaaccgcgca ggatgaagca atggcgctgc aggagaccct taagtcagct
      121 gaggcagcca gggatgaggc actttcgaag cttgcagcgg cagaaaccgc cgccatggtg
      181 atgaaggcac agacagaagg gctgacgctg gaagaactgg ccaggttcaa cggaaaggac
      241 ggccagcccg cctatatcgc cgttgacggc atcatttatg atgtaactga aagccgtgcc
      301 tggaaagacg gcatgcataa cggctttgaa gcaggcaagg atctgacgga agaaatcaag
      361 acaaagtctc ctcacgggat cggcaagctt gaaggcatta ctcaggtcgg taagctgaaa
      421 gcagaataaa gctgactcaa attaaaccct aacttttcga attaagtaat agtgatacct
      481 aactccgtta gcaaagcaag tgtcttcttg gaaatctccg atgtctgcca ggtatcggag
      541 attttgattt tgtggatata gttcaaatgg gatatcaggt cagcgataga gaacctggat
      601 aaaagctttt tatcgcgcag aaggttgtaa agcttatagc aaagaagcag ggatatatgg
      661 ttgatgaaag cccaaccttc cattgcggct tggctttgca gataactttt atcctgatcc
      721 atcaggttct ttaagaaatc gaaagtttgt tcgatgttcg tgcgttgttt atacaggcag
      781 tatacctctt gaggagaaag attgaggttg gtcttcagag cgatggtgcc gaacttgaat
      841 tgtttatcaa gcaggctgct gtctgtatag ttctcaatct tcttttcgat cctgagacaa
      901 tagtctttct cctcctgcgc gcgcagttca ctgtccagga aatagcatat cccgtcaccg
      961 gaatagtacc agatcggacg ggtgttaaac atgaaatgcc catcgaagtc cgccttgttg
     1021 cccgactcaa acctggtatg gctgtatgcc tcgctgttcc ttctgagcgg cacgatatat
     1081 ctcaaacctg cgctctccag cagttcaaag ttttcctttg agccaaaacc cttgtcggcc
     1141 actatcgtca tgtcctttat accgctttct tcgatacaga gcttgaaagc agtgacatct
     1201 ctgacattcc cagctacgat tctgtagtat gccggctgat ttgattcaac agagaaggcg
     1261 tagagtaagt ttacctgcgg gtcatattgg cgatgagcat tatatccaac tcgattatgc
     1321 tccattttct ccgatttcga tatgatcgac gtgccgtcaa acagtatatg ctcagttccg
     1381 ctcataaacg ccttcatgaa ctctgcaata gccgggcggt cgtctcccag ccgctgcaaa
     1441 aagctgctca tgctttttcc gcccatcttg agcccgctga ataggacaga aaggtatgaa
     1501 tgcgagtact cggcctcaag ccgcttgaat gggtgactgt gcagaagcct gagggctgct
     1561 gccgtgaata tggtctcaga ggacgggccg aaatgctcac ggagcttttc aagtatctca
     1621 gctcccactt gggacgcgag caaagcgccg ccgtaatgca aaacgagcgg aggatctgca
     1681 tctatccttg tcatgcgttc cattttgggt ataagtccgg actcggttat gcggccgatg
     1741 taagacttgg tgattttctt tgctcgcttt ttctccttgt cccattgaga agtcacttcg
     1801 tagagataat agttgttgcc gatctttgag atattagtgc ctggtctttt ctctttaagc
     1861 acccaatccg gatacatgtt tctcacctcg atattactta tgcaagtaat atgtttgtat
     1921 cataaaaaag accgaaacgc aagaggtttc agtctctttt tgaaatatta tatggcttaa
     1981 tgtgaaaagt tagggcttaa atggctggcg ctgctggatc agcatctctc cctcagcgtt
     2041 gaacaggcac acatgcacga ccaggtggta atcgcccgcc gccatctcgt ccccgcgccg
     2101 catggtccgc cccgtcaggc ggcggtcgcg gtcatataca tcccacatct ccatagcctg
     2161 cttcct
//
LOCUS       DAOSCC010000302         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_34046_length_2137_cov_4.875600, whole genome shotgun sequence.
ACCESSION   DAOSCC010000302 DAOSCC010000000
VERSION     DAOSCC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34046_length_2137_cov_4.875600"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLG72_11335"
     CDS             <1..440
                     /locus_tag="PLG72_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ81430.1"
                     /translation="KIRDSHIGFGIVTVDLTARRAVEIYKEEIPDGQLVDYVIASASF
                     PAFKRTIIDGRTFIDGALYNNLPINLVSNRGSRDIIVLRTYGMGIKRRVNTEGLNIIS
                     ISPSENLSSVMDFSNKSARRNLKLGYDDACRVLEEYLRTAGRY"
     gene            645..>2137
                     /locus_tag="PLG72_11340"
     CDS             645..>2137
                     /locus_tag="PLG72_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ81431.1"
                     /translation="MIFITAASYVVLTGIIIYLISELRSRRYRFGAEMKDTALDAEEL
                     RKHAEEMARNHPAGKHSRSLNWLIRRLNENYSLIYGIYRDLSRDVRESLPTPPSAEWL
                     LDNFYIIEEQVKLIRRNLSRGRYSRLPVLKSGYYRGYPRVYSIALEIVAHTNGSIDEK
                     SIMTFIQAYQSHSLLSMSELWAMPLMLRIALIESIRNTCEAIRVSRQEWRSAETLVSR
                     IIDAGADEQKIDRILKNEFNAAKPVTTSFAEHLTSRLRKHGKVLSTVTALLDQRLKAE
                     GLSTADLTLAEHKKQAETQVAIGNSITGLRLISELDWSDIFESLSKTEQILREDPDGV
                     YTLMDFESRDYYRHEVEKLARAFGIPETQVAQKAVGCAQEGGESPKDHVGFYLVGNGR
                     KILLDRISKTVRRRWRFRFSPLAKPKRLYVGLVISMTVFMTAYFMYYAWTKTPSLLPA
                     VLAGVFVLLPCTELALRFTNTIISHLYSPILLPKLELRSGIPENHAA"
BASE COUNT          571 a          500 c          584 g          482 t
ORIGIN      
        1 aaaagatcag ggattcccat atcgggttcg ggatagtcac cgttgacctc acggcgcgga
       61 gagctgtgga gatctacaaa gaggagatac cggacggtca gcttgtggac tatgtgattg
      121 caagcgcaag ttttcctgcg ttcaaaagga caataataga cgggaggacg tttatagacg
      181 gcgcgctgta caacaacctg ccaatcaatc tcgtcagcaa caggggatcc agggatatca
      241 tagtgctgcg gacttacggc atgggcatca aaagacgagt caataccgaa ggtctgaaca
      301 ttataagcat ctcaccttcc gaaaacctct cttcggtaat ggatttcagc aacaagtctg
      361 cgcgccgcaa cctgaaactg ggctatgatg atgcttgcag ggtactggaa gagtatttga
      421 ggactgcagg ccggtattga gcattgagga cggcaagctg ccattgggcg ttgcaggccg
      481 cgggatgccg ctggtcatag cgtcctgcag gctgccattg taaattgcag atcgcagcag
      541 ctttgaaatc acaatctgtg gaatcatttg gagcataaca tgataatcac cgtttcaggg
      601 catgctatat gaacgaaaat tgcatggaat ggtgattttt ccggatgatt ttcataacag
      661 ctgcatcata tgtggttctg acaggtatca ttatatatct catttcagag cttcgttccc
      721 gcagatacag attcggtgcg gaaatgaagg acactgcact tgatgctgaa gagcttagga
      781 aacatgcgga agagatggcc aggaaccatc cggcgggaaa acattcaaga agtctcaact
      841 ggcttattcg gcggcttaat gaaaactaca gcctgattta cggcatctac agggatctca
      901 gcagggatgt gagagaatcg ctgccgacgc ctccctcagc tgaatggctg cttgataatt
      961 tctacataat cgaagagcag gtcaagctga taagaaggaa tttgtccagg gggcgttatt
     1021 caaggctgcc tgtccttaaa agcggatatt acaggggcta tccgagagtc tattcgattg
     1081 ctctggagat agtcgcacat accaacggaa gcatagatga aaaaagcata atgacattca
     1141 tacaggcata ccagtctcat tcgctgcttt cgatgagtga actgtgggct atgcctctca
     1201 tgctgcgcat tgcgcttatc gaaagcatcc ggaacacatg cgaagccatc agggtgtcga
     1261 gacaggagtg gcgaagcgcg gagacgctcg tgtcgcgcat tatcgatgcc ggtgcggatg
     1321 aacagaaaat cgaccggata ctaaagaatg agttcaatgc cgccaaacct gtaacgacgt
     1381 catttgccga acatttgacc agcaggctga gaaaacacgg caaagttctt tctaccgtga
     1441 cggcgctgct cgatcagcgg ctgaaagccg agggcttgtc cacagcggat ctcacccttg
     1501 cggaacacaa aaaacaggct gagacgcagg tcgccatagg caactcgatc acaggcctga
     1561 ggctcatttc tgaactggat tggtccgaca tttttgaatc gctcagcaaa accgagcaga
     1621 tcctcaggga agatcccgac ggtgtctaca ccctgatgga ttttgaatcg agggactatt
     1681 accgtcatga agtggaaaaa ctggccaggg ccttcgggat cccggagaca caggtggcgc
     1741 aaaaggctgt cggctgtgcg caggagggcg gcgaaagccc aaaggaccat gtcggttttt
     1801 accttgtagg aaacggacgg aaaatactgc tggaccggat cagcaaaacg gtccggagaa
     1861 gatggaggtt ccgtttttct ccgcttgcaa agcccaaaag gctctatgtt ggactggtta
     1921 tatccatgac ggttttcatg acggcctact tcatgtatta tgcatggacg aagaccccgt
     1981 cgctgctgcc ggcggtcctg gccggggttt ttgtgctgct gccgtgtacc gaactggccc
     2041 tgcgatttac gaatacgata ataagccatc tttacagccc gatcctgctg ccgaagcttg
     2101 aactgagaag cggcatcccg gaaaatcacg cggcatt
//
LOCUS       DAOSCC010000303         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_35596_length_2072_cov_5.062965, whole genome shotgun sequence.
ACCESSION   DAOSCC010000303 DAOSCC010000000
VERSION     DAOSCC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35596_length_2072_cov_5.062965"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLG72_11345"
     CDS             complement(<1..789)
                     /locus_tag="PLG72_11345"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOJ81432.1"
                     /translation="MKILVINTGSSSLKYQLIDMENESVLAKGLCDRIGIDNSFIKHT
                     RTGNDPVVIEADMTNHRAAIRQVIDVLTDEKIGVIKNMSEIAAVGHRIVHGGEKFHDS
                     VIIDDSVMAAIRECIDLAPLHNPPNIVGIEACQHIMPDTPMVAVFDTAFHQTLPRYAY
                     LYALPYEMYEKHGIRKYGFHGTSHKYVAQRAAVMLGRPLGELKLVTCHLGNGASICAV
                     EYGRSVETSMGFTPLDGLAMGTRSGSVDPAVVKFLMENEKMSPKE"
     gene            complement(805..1797)
                     /gene="pta"
                     /locus_tag="PLG72_11350"
     CDS             complement(805..1797)
                     /gene="pta"
                     /locus_tag="PLG72_11350"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOJ81433.1"
                     /translation="MKFLEQISERAKKDIKTIVLPESSDIRTIKAAATVLEKGIARIV
                     LVGKEAEIKKLAGDLDISKARIVDPETSPDFERYANALYEIRKSKGMTPEQARELVKD
                     ELYWGVMMVKMGEADGMVAGAIHSTAQTLRPALQILKTAPGTKLVSAFFVMVVPDCEY
                     GSNGTFIYADSGLVENPDAEQLSEIAISSAKTFRNLIQDEPVVAMLSYSTYGSAESEL
                     TRKVVEATKLAKQKAPDLLIDGELQADAALVPSVGQFKAKGSPVAGKANVLIFPDLNC
                     GNIAYKLTQRLAKAEAYGPITQGIAKPVNDLSRGCSAEDIVGVVAITAVQAQMS"
BASE COUNT          463 a          553 c          466 g          590 t
ORIGIN      
        1 ttccttcggc gacatcttct cattctccat caggaacttg accaccgccg gatccactga
       61 accgcttctg gtacccatgg cgaggccgtc aagcggcgta aagcccatgc tggtctcaac
      121 ggatctgcca tattcaacgg cacatatgct tgctccgttg ccaagatggc atgttaccag
      181 cttcagttcg ccaagcggcc tgccgagcat gacagcggcc ctttgggcga catacttgtg
      241 cgacgtcccg tggaatccgt atttcctgat cccgtgcttc tcatacatct catacggcaa
      301 tgcatataaa taggcatatc ggggaagtgt ctggtggaat gccgtatcga acacagcaac
      361 catcggagtg tccggcatta tatgctgaca tgcctcaatg cctacgatat tcggcggatt
      421 atggagcggc gccagatcga tgcactctct gatagcagcc atcacactat catcgattat
      481 caccgaatca tggaatttct ctcctccatg gactatacgg tgtcccaccg ccgctatttc
      541 ggacatgttt ttgatgaccc cgatcttttc atctgtcagc acatcgatga cctgtctgat
      601 cgcagccctg tggttcgtca tgtcggcctc gatgactacg ggatcgttcc ctgtcctggt
      661 gtgctttatg aacgaattgt ctatgccgat cctgtcacaa aggcccttgg ccagcacaga
      721 ctcgttttcc atgtctataa gctggtattt caatgatgaa ctgccggtgt ttatgactaa
      781 tattttcatt tacatacagc tcctttaact catttgtgcc tggactgccg ttattgcaac
      841 gacgcccacg atatcttcag ccgaacagcc tctggagagg tcattgaccg gcttggctat
      901 accctgtgtg atcggtccgt aagcttcggc cttggcgagc ctctgtgtca gtttatatgc
      961 gatgttgccg cagttgaggt cggggaaaat gagcacattt gccttgccgg caacaggact
     1021 tccctttgcc ttgaattgtc cgactgacgg tacaagcgcc gcatctgcct gcagttcgcc
     1081 gtcgatcagc agatccggag ccttttgttt tgccagtttt gtcgcttcca caactttccg
     1141 ggtcagttcg ctttcagcgc tgccatacgt ggaatatgaa agcatggcta ctaccggttc
     1201 atcctggatc aggttcctga atgtcttggc tgatgagatc gctatttcag acagctgttc
     1261 tgcatcggga ttttcaacca gtccgctgtc agcataaata aaagtgccgt tgctgccgta
     1321 ttcacagtca ggcactacca ttacgaagaa tgccgaaacc agttttgtgc ccggagcggt
     1381 cttgaggatc tgcaaagccg gcctgagcgt ctgagccgtc gaatgtatcg ctccggcaac
     1441 cattccgtcc gcttcgccca tctttaccat cataacgccc cagtagagtt catcctttac
     1501 aagctcgcgt gcctgttccg gcgtcatccc ctttgacttc ctgatttcat aaagcgcatt
     1561 ggcatacctt tcgaaatcag gtgaagtttc aggatcgact atcctcgcct tcgagatgtc
     1621 cagatcaccg gcaagctttt tgatttccgc ttccttgccc acaaggacga tcctggcgat
     1681 acccttttcc agcaccgttg ctgcggcctt gatcgtcctt atgtcagagc tttcaggcag
     1741 cacgatagtt ttgatatcct ttttcgcgcg ttcactgatc tgttcaagaa atttcaattg
     1801 aaagacccct ttcacataga tgtacaggac cgattttatt ctgccctgat ttgatgtgtt
     1861 tagtccgata taaaccgaag cgtataatta ttacctggtt ctaacatcag cttatatcag
     1921 gctctcaagc catgtttatt atatacaaaa cgtttattgt atacaagata caattagtac
     1981 aaaatggggt catattcgag aaatgaaaaa atatggatca ggatcgggct tcagacctgc
     2041 tgaatgatat tacaaaaaag gtcctgtatt tg
//
LOCUS       DAOSCC010000304         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_36426_length_2038_cov_3.384771, whole genome shotgun sequence.
ACCESSION   DAOSCC010000304 DAOSCC010000000
VERSION     DAOSCC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36426_length_2038_cov_3.384771"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..769)
                     /gene="pth"
                     /locus_tag="PLG72_11355"
     CDS             complement(71..769)
                     /gene="pth"
                     /locus_tag="PLG72_11355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ81434.1"
                     /translation="MFPSVRSLLRKNKPAEDAGPGKLTVIVGLGNPGRKYENTRHNVG
                     FMAVDLIAQRYGIKVNRLRHKALTGDGMIDGKRILLAKPQTYMNLSGESVRDIVEYYK
                     VPMENLIIIYDDIDLPAGVLRIRPKGSSGTHNGMKSVIYHLQSDSFPRIRIGIGKAPE
                     GWDLADYVLGEFGSDEADKVADCIRRAAGAAAAIVAEGIEKAMSRYNGNGDKFESKPG
                     DKSREDKTDGGRTT"
     gene            complement(732..1694)
                     /locus_tag="PLG72_11360"
     CDS             complement(732..1694)
                     /locus_tag="PLG72_11360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ81435.1"
                     /translation="MNLHGKDIKIFAGNSNLPLAKEIAERIGLPLGMATVGKFSDGET
                     CVNIGEVVRGSDVFVIQSTCTPVNDNLVELLIMIDALKRASAGRITAVMPYFGYARQD
                     RKAKARDPISAKLVANLITTAGADRVLTMDLHTPQLQGFFDIPVDHLLGVPVLAKHFQ
                     EKFPEHNDMVAVSPDVGSVTRTRKFAEWLDIPIAIIDKRRPKANVSEVMNVIGDIRNK
                     RVVLIDDLIDTAGTVANASEALMEMGATEIFACCTHGVLSGPAFERIESSPIKELVML
                     NTIPLPENRPTDKITSISIAPLFAEAIERIYGDVSISTLFTQKE"
BASE COUNT          412 a          585 c          459 g          582 t
ORIGIN      
        1 agtaaccggt tgtattcctc cagttccagc agcggttcga taaagttcag cataaagttc
       61 ctgatcccgc ctaagtcgtt ctgcctccgt cagttttgtc ttccctggac ttatctccgg
      121 gtttgctttc gaatttatcc ccatttccgt tatacctgct catggccttt tctataccct
      181 ccgcaactat tgcagctgcg gccccggcgg ccctcctgat gcagtctgcg actttatccg
      241 cctcatcgct gccgaattcg ccgaggacat agtcggcaag atcccagcct tcaggagcct
      301 ttccgatgcc tatcctgatc cttgggaagc tgtcggactg caggtggtat atcaccgatt
      361 tcatgccgtt atgggtgcct gaactgccct tcgggcgtat tctgagcact cctgccggca
      421 ggtcgatatc gtcatatata ataatcaggt tttccatcgg taccttgtaa tattcaacga
      481 tatcccgcac actttctccg ctcagattca tgtatgtctg cggttttgca agaaggatcc
      541 ttttcccgtc tatcatgccg tcgccggtca gcgctttatg cctgagccgg ttcactttta
      601 tcccgtacct ctgtgcgatc agatcgacag ccatgaatcc cacgttatgc cttgtgttct
      661 cgtacttcct ccccgggttc ccaaggccaa cgatgactgt cagttttccc ggtccggcgt
      721 cttcagccgg cttattcttt ctgagtaaag agcgtactga tggaaacatc tccatagatc
      781 ctttctattg cttcggcaaa caaaggagcg atcgaaatgg atgttatctt gtcagtcggc
      841 ctgttttcgg gaagcggtat agtattgagc atgaccagtt cttttatggg tgagctttct
      901 atcctttcga atgcgggacc ggacagtact ccatgggtac agcaggcaaa tatctcagta
      961 gcgcccattt ccatcagcgc ctccgatgcg tttgcaacag tgcccgccgt atcgataagg
     1021 tcatctatga ggaccactct cttgttcctg atgtcaccga taacattcat cacttcactg
     1081 acatttgcct tcggccttct cttgtcgatt atggctatcg ggatatccag ccactcggcg
     1141 aacttccttg tacgggtcac gctgcctaca tcaggggata cagccaccat atcgttatgc
     1201 tccgggaatt tctcctggaa atgctttgcc aataccggca ccccaagcag gtgatccacc
     1261 ggtatgtcaa agaaaccctg gagctgcggc gtatgcaggt ccatggtcag caccctgtct
     1321 gctccggcag tggtgatcag gtttgcaacc agttttgcag atataggatc cctggccttt
     1381 gccttcctgt cctggcgcgc atagccaaaa tacggcataa ccgccgtgat cctgccggcc
     1441 gatgctcttt tcagggcatc gatcatgata aggagttcga ccaggttgtc gtttacaggc
     1501 gtgcaagtgg actgtataac aaaaacatcg gagcccctga cgacttcgcc aatatttacg
     1561 catgtttctc catcgctgaa cttcccgact gttgccattc ccagcggcag tcctatcctt
     1621 tcggcgatct cctttgccag cggcaaattg gagttgcctg cgaagatctt gatatccttg
     1681 ccgtgaagat tcataactat actcctttgg tttgatttta tagctattac attgtaattg
     1741 ttaacgtttt aacaagactt cgatcccaca ccagtatacg ttttcccatg cgtttttccg
     1801 cagatgtcaa tagcctccgc ccgcaggctc ccccttgttc gctgcgggcg aaggtgatcg
     1861 atccctgcat atgcttcatt cgatgcatcc ctgaagccga tccgcaagta tggactccag
     1921 catctcaagc tgggcccggt cgttggcccc gagcacctcg gtgctgtcct gcactttgta
     1981 aacgcctacc ttaagccccc tggcaagcat tatttccaat gtgtcagtca gataatac
//
LOCUS       DAOSCC010000305         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_37097_length_2013_cov_3.460674, whole genome shotgun sequence.
ACCESSION   DAOSCC010000305 DAOSCC010000000
VERSION     DAOSCC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37097_length_2013_cov_3.460674"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..>2013
                     /locus_tag="PLG72_11365"
     CDS             275..>2013
                     /locus_tag="PLG72_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ81436.1"
                     /translation="MKKGINISIKNGRRVVEGLGYYEPRIVRDLKELVRGSAERYGAR
                     PAFKFRDKAGNIVFKTFNDLNSDVDCLGTAMMSLGMKDMRISIISENRYEWGVCYFAT
                     VNGTGVAVPLDRHLPKNEIENLIARGKVEAIFYSPVYHDTMVDISKDNDAVKFFICME
                     KLEPRQAYLSGQDGREAPHGAEGDDRSHNRSGDGVPHGRGVGEEGGSSPVGYPMFLSL
                     PDLVEKGRKLIGSGDRSFIDAPIDREKMSVLLFTSGTTSTSKGVMLSHSNLASNVSSI
                     TTIIKIDERDTHLSLLPLHHTFENTVGLLFILHCGACIVYGDGIKHIVQNLKEYNVTL
                     LVAVPAIFEAMYRKLQDGIRKSGKEKAFKILIKVSNLLRSVGIDLRRKLFRSVFEKIG
                     PNLRIAVSGAAALDREVVTGFEQIGFRLLQGYGLTEASPVVATNNDFVNVPGTIGHPM
                     GGVKVAIDSPDENGIGEIVTRGGNVMLGYYEDPEATAEAFTEDGWLRTGDVGYIDDEG
                     FIRITGRAKSMIVLTSGKKAFPEEYEVKLNNIPGVRDSFVWGNVAPDGDVQICAKLVV
                     DSENAVDSNGRLLP"
BASE COUNT          544 a          430 c          577 g          462 t
ORIGIN      
        1 aaactgcgca tagttggaca tgaaggtgca tctttgatgc accttcagtt gtattttcag
       61 tattggcttt tttgtacata gaatagaaaa ttctgctgtt gttctgtaaa agacgctgat
      121 gattcagaaa atctggcttt cccttgtcta ttgcgtttta gcaaaatttc agttataata
      181 attattacct agtaataata tttagtaata tgatgtagtg ataacaactt ggattctgca
      241 gttcacacca tattttgaac gagggagggc aggtatgaag aaaggtatca atatcagcat
      301 caagaacggg agaagagtcg tcgaaggcct tggatactat gagccgagaa tcgtgagaga
      361 tcttaaggaa ttagtaagag gaagcgcgga aagatacggc gccaggcctg cttttaaatt
      421 cagggacaaa gccggcaaca tcgttttcaa aacattcaat gatctcaatt ccgacgtgga
      481 ttgcctcggc actgcaatga tgtcgctggg catgaaggac atgaggatat cgataatcag
      541 tgaaaaccgg tatgaatggg gcgtatgcta ttttgcaacg gtaaacggga ccggagtggc
      601 tgtgccgctt gacaggcacc ttcctaaaaa cgagatagag aacctcattg caagggggaa
      661 agttgaagcc attttctaca gcccggttta tcatgatacc atggtggaca tctcaaaaga
      721 caatgacgca gtgaagtttt tcatatgcat ggaaaaactc gaacccaggc aggcttattt
      781 gagcgggcag gacggccgcg aagcgccgca cggcgccgaa ggagacgaca ggtcgcacaa
      841 ccgcagtgga gacggagtac cgcacggccg cggagtgggc gaagaggggg gaagttcgcc
      901 ggtcggttat cccatgttcc tctcactgcc cgatctggtc gaaaagggca ggaaactcat
      961 tggatccggg gacaggtcgt ttatcgatgc gccgatagac agggaaaaga tgtcggtgct
     1021 gctgttcact tccggcacga ccagcacatc aaagggcgtt atgctgtcgc actccaacct
     1081 ggcgtccaat gtttcgtcaa tcactaccat aataaaaatc gatgaaaggg acacccacct
     1141 gtcgctgctc ccgctgcacc atacgttcga gaatacggtc ggccttctgt ttatactgca
     1201 ctgcggcgcc tgcatcgttt atggcgacgg tataaagcat atagtgcaaa acctgaagga
     1261 atacaacgtg acactgcttg tggcggtacc ggctattttt gaagcaatgt acaggaaact
     1321 tcaggatggg ataagaaagt cgggcaagga gaaagcattc aaaattttga ttaaggtatc
     1381 gaacttgctc agatcggtcg gaatagatct gagaagaaag ctgttcagga gcgtcttcga
     1441 gaaaatcggt ccgaatctca ggatcgctgt ttcaggcgct gcggcgcttg acagggaagt
     1501 ggtgacaggc tttgaacaga tcggtttcag gctcctgcag ggatatggcc tgacggaagc
     1561 ttctcctgtc gttgccacga acaatgattt cgtgaatgtg ccgggcacca tcggacatcc
     1621 gatgggcggc gtcaaggtgg cgatcgattc gcctgatgag aacgggatag gcgagatcgt
     1681 gacccggggc gggaatgtga tgcttggtta ttatgaagac cctgaagcca cggcagaggc
     1741 cttcacggag gatggctggc tcaggacagg cgatgtgggg tatatcgacg acgaaggttt
     1801 cataaggatc acaggccggg cgaaatcgat gatagttctg actagcggga agaaggcgtt
     1861 tcctgaggaa tacgaggtca agctgaacaa tatcccgggc gtcagggact cgttcgtatg
     1921 ggggaacgtc gcccccgacg gagatgtgca gatctgcgcg aaactcgtgg tcgacagtga
     1981 aaacgctgtc gacagcaatg gcagactgct tcc
//
LOCUS       DAOSCC010000306         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_38848_length_1950_cov_3.460686, whole genome shotgun sequence.
ACCESSION   DAOSCC010000306 DAOSCC010000000
VERSION     DAOSCC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38848_length_1950_cov_3.460686"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1337)
                     /locus_tag="PLG72_11370"
     CDS             complement(60..1337)
                     /locus_tag="PLG72_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ81437.1"
                     /translation="MRIVGIVAEYNPFHYGHMYHLQAARRVTGADCVVVVMSGNYTQR
                     GEPAVVDKWARAEMALLSGADLVIELPVAYAMGSAEYFAFGAVRLLDSLGAVDCMCFG
                     SESGDIETLWGIASILATEPEEYKKLLREQLSAGLSFPSARQKALSAYLRDKSGKDSF
                     TRILKSSNDILGVEYLKALLRIGSRIQPVTICRVGSSYNSTELTGKFSSATSIRKILG
                     VNKWCCARKLVESSMPTESLSVLEREIELGRGPVFPSDFGSTIISLIRRIPAGELRNL
                     PYMEAGLENRIRLAAEKSGTYDELVGSITTRRYTITRVQRILFSLLIGLDSRKFESFN
                     SLGGPAYIRILGFNGTGRKLLSSIRGRTKLPLITKTADFKSSDIPGVSAMLELESNAT
                     DLYVMGFENPYFRASGSEFTRNVINIDASAKNR"
     gene            complement(1371..>1950)
                     /locus_tag="PLG72_11375"
     CDS             complement(1371..>1950)
                     /locus_tag="PLG72_11375"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOJ81438.1"
                     /translation="DSPNPEALEKALKIIKAKPIINSITLEKERYDSILPLVLEYSTR
                     VIALCMDDGGMPETTEERVAIADKLIAGLTAKGVDISDIFIDPMVRPIGTGSHYGVVA
                     LETIRRVRSEFPEVHITCGLSNISFGLPARKVINQAFMIAAMTCGLDSAILDPLDKRL
                     MAYIYESEAMLGKDDFCMNYLAKFREGLIDLP"
BASE COUNT          433 a          508 c          488 g          521 t
ORIGIN      
        1 ctatatggcg gaaaaaatcc ttccattaag caaaaaaata aaataacatt tgtaatattt
       61 tatctgtttt tcgcggaggc gtcaatattt atgacattgc gggtaaattc actgcctgat
      121 gctctgaaat acgggttttc gaaacccatg acgtaaaggt ccgtggcatt cgattcaagt
      181 tcaagcattg ctgaaacgcc agggatatcc gatgatttga agtccgcagt ttttgtgatc
      241 aggggcagct tggtcctgcc cctgatggat gacaggagtt tcctgcctgt gccgttgaat
      301 ccgaggatcc ttatatatgc aggtccgccg agactgttga aactttcgaa tttcctgctg
      361 tcaaggccta tcaggaggct gaacagtatc cgctggaccc ttgttatcgt atagcgcctg
      421 gtggtgatgc tgccgaccag ttcatcatat gtacctgatt tttctgctgc cagcctgatc
      481 ctgttttcaa ggccggcttc catataaggg aggtttctca gttcaccggc aggtatgcgc
      541 ctgatcagtg atattatcgt ggagccgaag tctgatggga aaacaggccc tcttccaagt
      601 tcgatttccc gttccagcac agacagggat tccgtgggca tggaggattc gacgagtttc
      661 cttgcgcaac accatttgtt gacacccagg atttttctta tagaggtggc gctgctgaac
      721 ttgccggtca gttccgtcga gttgtaactg ctgccgaccc tgcatattgt aacaggctgt
      781 attctgcttc cgatccgcag cagtgccttg agatattcta ccccaagtat atcgttggag
      841 cttttcaaaa tccgggtaaa actgtctttt ccggatttgt cccgcaaata ggctgaaagg
      901 gccttctgcc tcgccgaagg aaaggaaagc ccggcggaaa gctgttccct gaggagcttt
      961 ttatattctt cgggttctgt tgcaagaatg gaggcgatac cccacagggt ttcgatatcc
     1021 ccggattcgc tgccgaaaca catgcaatcc acggctccca ggctgtcgag cagcctgacg
     1081 gccccgaacg caaagtattc ggcactgccc atggcatacg caacgggcag ttcgatcacc
     1141 agatcggctc cggaaagcaa tgccatttcg gccctggccc acttgtcgac cacggccggc
     1201 tcgccgcgct gggtatagtt gccgctcatc acgactacta cacaatcggc cccggtcacc
     1261 ctgcgggcag cctggagatg gtacatgtgt ccgtagtgga atggattgta ttctgctacg
     1321 ataccgacga ttctcatccg atcctcccgg cattcaaccc aatacctgca tcagggcagg
     1381 tcgataaggc cttctctgaa tttcgcaaga tagttcatac agaagtcatc cttgccaagc
     1441 attgcttccg attcataaat gtaagccatc agccttttgt ccagcggatc cagtatcgct
     1501 gaatccaggc cgcaggtcat cgcagctatc atgaaagcct ggttgatgac tttcctggca
     1561 ggcaggccaa aggatatatt gctcaggccg catgttatgt gcacttcggg gaattcggaa
     1621 cggacacggc ggatcgtctc aagggccacg acgccgtaat gcgacccggt gcctatcggc
     1681 ctgaccatgg gatcgatgaa tatgtcagat atgtccactc cttttgccgt aagtccggca
     1741 atgagtttat ccgctatggc gaccctttct tccgtcgttt ccggcatgcc gccgtcatcc
     1801 atgcacagtg cgatcaccct tgtactgtac tctagcacga ggggcaggat ggaatcatat
     1861 ctttcctttt caagcgttat tgaatttatg ataggtttgg cctttattat cttcagcgct
     1921 ttttccaacg cttccggatt gggtgagtcg
//
LOCUS       DAOSCC010000307         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_38989_length_1945_cov_3.969312, whole genome shotgun sequence.
ACCESSION   DAOSCC010000307 DAOSCC010000000
VERSION     DAOSCC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38989_length_1945_cov_3.969312"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..493
                     /gene="def"
                     /locus_tag="PLG72_11380"
     CDS             11..493
                     /gene="def"
                     /locus_tag="PLG72_11380"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ81439.1"
                     /translation="MAIRNIRKEGDEVLRKRSREVTEINDNILILLDDMADTMYKANG
                     AGLAAPQVGVLRRVVVIDIGEGLVELINPVIRKQSGKQTTVEGCLSIPGVWGEVERPA
                     KVVVEALNRNGEKIRIEGEGYMAQALCHEIDHLDGILFTDKVIRFLDEEELEKMEQES
                     "
     gene            490..1440
                     /gene="fmt"
                     /locus_tag="PLG72_11385"
     CDS             490..1440
                     /gene="fmt"
                     /locus_tag="PLG72_11385"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ81440.1"
                     /translation="MRIVFMGTPDFAVPCLEALVSSGYDVAAVVTQPDKPVGRSQSVL
                     KPPAVKEAAIRLGISNILQPAKVRTPEFAETIRRLEPDVIVTAAYGKILPKSILDIPK
                     HGCINVHASLLPKYRGAAPIQWALINGESKTGITTFFMDEGMDTGDIILMKETEIDPD
                     MNAQELFDRLKFLGAEALLETLEALRNGTITRTPQDHSKATYVTMMTRETGLIDWNKS
                     AVEIHNLVRGTNPWPGAYTFYKGKRLKIWKTAVCKDPDVISAVSGEARPGTLLKCRKQ
                     DLIAATGSGLLAITELQFENARRMSVAECGHNMDVGEVFG"
     gene            1501..1755
                     /locus_tag="PLG72_11390"
     CDS             1501..1755
                     /locus_tag="PLG72_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81441.1"
                     /translation="MSFFFVQETFSIDLYSGMISLCRAILSSGLKPLSGMTAFTVTKS
                     FCGMMLFSRMVPMNAGVFITAAALLAAGRRKMAEGTLAVG"
BASE COUNT          537 a          416 c          572 g          420 t
ORIGIN      
        1 gagtggatgt atggctataa ggaatataag gaaagaagga gacgaggtcc tgcggaaaag
       61 gtcccgcgaa gttacggaaa tcaatgacaa tatactcata ctgctcgatg atatggcaga
      121 tactatgtat aaggcaaacg gggcaggcct tgccgcaccg caggtaggtg tgctcaggag
      181 agtcgtggtc atagatatcg gagaaggact ggtggaactg ataaacccgg tcatcaggaa
      241 gcagagcggc aaacagacca ctgtagaagg ctgtctgagc ataccgggcg tctggggaga
      301 ggtcgaacgc cctgcgaaag tagtggtgga agcgctcaac cggaacggag aaaagatcag
      361 gatcgagggc gaaggttaca tggcccaggc tctatgccat gagatagatc atcttgacgg
      421 gatccttttc acggataagg tgatacgttt cctcgatgaa gaagaactgg aaaagatgga
      481 gcaggaatca tgagaatagt attcatggga acacctgatt ttgccgtgcc ttgccttgaa
      541 gcgctggtca gcagcggtta cgatgtggcg gccgtcgtta cccagcctga caaacctgtg
      601 ggaagaagcc agagcgtgct gaagccgcct gccgtcaagg aggctgccat aaggcttggc
      661 atcagcaata tcctgcagcc tgcgaaggtc aggacgccag agttcgccga aacgatcagg
      721 agactcgagc cagatgtcat agtaacggct gcttatggaa agatactgcc gaaatccata
      781 ttggacatac cgaagcatgg atgtatcaat gtgcatgcat cattgctgcc gaagtacagg
      841 ggagcggccc ccatacaatg ggcacttatc aacggtgaaa gcaagacggg aatcacgaca
      901 tttttcatgg atgaaggcat ggataccggt gatatcatcc ttatgaagga aacagaaata
      961 gatcctgaca tgaacgcaca ggaactgttt gaccgtctta agttccttgg cgccgaagca
     1021 ctgctcgaaa cccttgaagc cctcaggaac gggacgataa cgaggacacc gcaggaccat
     1081 tcaaaggcga catatgtgac tatgatgacg agagagacag gtcttataga ctggaataaa
     1141 agtgcagttg agatacataa cctggtcagg ggcacaaatc cgtggcccgg cgcttatacg
     1201 ttctacaagg gtaagaggct caagatatgg aagacggcgg tctgcaagga tcctgacgtg
     1261 atttcggcgg tgtcgggaga ggccaggccg ggcactctgc tgaagtgccg gaaacaggac
     1321 ctgatcgcag ccacaggcag cggtttgctg gccattacag agcttcagtt tgagaacgca
     1381 agaaggatga gtgttgctga gtgcggccac aacatggatg tgggtgaagt gtttggataa
     1441 acgtgtcaaa ggcttcccgg cccgcctatt tcgccggtgc ctgccatcgg cgtcccggtc
     1501 atgtctttct tcttcgttca ggaaaccttt tcaattgatt tatacagtgg gatgatatcg
     1561 ctctgcagag ctatattatc cagtgggtta aaaccattga gtgggatgac agctttcact
     1621 gtgacaaaat cattctgtgg gatgatgctt ttcagcagga tggtacccat gaacgctggt
     1681 gtattcataa ctgccgcggc gctgttggct gcggggagga ggaaaatggc ggaaggtacg
     1741 ttagccgtcg gctgatctgc agccgtctta cggttgaaca gccaattacg ggatgataaa
     1801 ctgccgcttg ccggattatg atgaagtgtg atattgcgag agaagctgca ctgaaaatac
     1861 tgtatgaaat aaatgaaaaa ggcgcttatt caaatatagc gctgaacaag tatctctcct
     1921 tgcatgagct ttctgaaagg gacag
//
LOCUS       DAOSCC010000308         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_40682_length_1891_cov_4.400327, whole genome shotgun sequence.
ACCESSION   DAOSCC010000308 DAOSCC010000000
VERSION     DAOSCC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40682_length_1891_cov_4.400327"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLG72_11395"
     CDS             <1..423
                     /locus_tag="PLG72_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ81442.1"
                     /translation="YVRGLISWLGFRQTGVEFVREKRLAGETKYSMKKMFKLAWDGIT
                     AFSNAPLKLATYIGVSISAVSFLYLLVVICQKLFTDTTVQGWASLVAISLFFNGVILI
                     VLGIIGEYIRRIYDEAKGRPIYIISEKIGFDEGHDEKD"
     gene            398..850
                     /locus_tag="PLG72_11400"
     CDS             398..850
                     /locus_tag="PLG72_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ81443.1"
                     /translation="MKDMMKKISAIMSKAGISREEILRLLKFAVIGVSNTLVDYLVFF
                     LLNNILLTGYGVNQAISYTCGMLNSFVLNRRWTFSDFRRNRKLPEQFLLFMAINGISL
                     AVTVGAMAMLAYTLGMNVYIAKALVILIAQAINFLGYRLWVFGKPNNA"
     gene            988..1061
                     /locus_tag="PLG72_11405"
     tRNA            988..1061
                     /locus_tag="PLG72_11405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1020..1022,aa:Gly,seq:tcc)
     gene            1125..1201
                     /locus_tag="PLG72_11410"
     tRNA            1125..1201
                     /locus_tag="PLG72_11410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1159..1161,aa:Arg,seq:tct)
     gene            complement(1313..>1891)
                     /locus_tag="PLG72_11415"
     CDS             complement(1313..>1891)
                     /locus_tag="PLG72_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81444.1"
                     /translation="IDLMSKRIPATLIVYDILYCKDKTVTEMPLIERKGLLEENTVES
                     DSLAISRFVRGTGTALFEQVVNQELEGIVAKKIDGIYVQGRSRLWLKSKRRQDMDLLL
                     CGYMEGKGFLFCDNDLKYRISIRSVDKNTFDFLYKYIIEHRVDNSPLKSVPSNLKGAI
                     WAKPELYCVVEYMQEMESGYLREAVFKGIKMD"
BASE COUNT          474 a          441 c          460 g          516 t
ORIGIN      
        1 tacgtcaggg ggctgataag ctggcttggt ttcaggcaga ccggggtcga attcgtcagg
       61 gagaaacgcc ttgccggtga aaccaagtac tctatgaaaa agatgttcaa gcttgcttgg
      121 gacggcatca ccgcattttc caatgcgcca ttgaaacttg ccacttatat aggagtcagt
      181 atatcggctg tcagtttcct gtatttgctg gtcgtgattt gccagaaact gttcacggac
      241 accactgtac agggctgggc ttcccttgtg gccatttcat tgtttttcaa cggcgtgatc
      301 ctgatcgtgc tcgggatcat tggcgagtac atccgcagga tctatgacga ggcaaaggga
      361 aggcctatat acatcatcag tgagaagata gggttcgatg aaggacatga tgaaaaagat
      421 tagtgcaatt atgtcaaaag cagggataag cagggaggaa atactgaggc tgctgaagtt
      481 tgccgtcatt ggagtttcca acacacttgt ggattacctg gtgttttttc tgcttaacaa
      541 tatcttgctg acaggctacg gggtcaacca ggcgatctca tatacctgcg gcatgctcaa
      601 cagctttgtc ctgaacaggc gatggacgtt cagcgatttc aggagaaaca ggaaactgcc
      661 ggaacagttt ttgctcttta tggctatcaa cgggatttca ctggctgtta cggtcggggc
      721 aatggcaatg cttgcatata ccctgggaat gaatgtatat atagcaaaag cgctggttat
      781 cctgatcgcg caggctataa actttcttgg atacaggctg tgggtatttg gaaaacctaa
      841 taatgcctga catattccat cttacgggct gccggccgaa aacagccgtc cgacagtgcc
      901 tcgcagcctg ataaattgca aaattacgtc attgacaaat aaaacggccg acgatataat
      961 atgaaagtcg gatgtcaagc ggtacgtgcg ggtgtagttc aatggtagaa catcagcctt
     1021 ccaagctgat tgcgtgggtt cgattcccat cacccgctcc acatggttat ccgggcagga
     1081 tcggcgccgg ccgtagaact gccgtagtat agatgaataa atgtgtgcca ctagctcagc
     1141 tggatagagc aacggacttc taatccgtag gtcgggggtt cgaatccctc gtggcacgcc
     1201 aggtaaaacc ccacgggctg taagtctgtg aggttttgtt gtatttgagc aaaggtcggc
     1261 aaactatgac cacttttttg acacgttccc tttgtatcat agctgccgtc cgtcagtcca
     1321 tttttatccc cttgaagacc gcttctctta aatatcccga ctccatctcc tgcatatatt
     1381 ccactacgca atacagctca ggttttgccc atatggcccc tttcaaattc gaaggcacgg
     1441 atttgagagg cgaattgtcc actctgtgtt caatgatata tttgtaaagg aaatcaaatg
     1501 tatttttatc gacgctgcga atggagatcc tgtatttaag gtcattgtcg caaaaaagaa
     1561 atcccttgcc ttccatataa ccgcacagca gcaggtccat atcctgtctg cgtttgctct
     1621 tgagccaaag ccttgaacgg ccctggacat agataccatc gatttttttc gcaacgatcc
     1681 cttcaagctc ctggtttacg acctgttcga aaagtgcggt cccggtgcct ctcacaaacc
     1741 tggaaatcgc cagactatcg ctttcgaccg tattttcctc aagcagcccc tttctttcaa
     1801 taagcggcat ctctgtgacc gttttgtcct tgcagtaaag gatgtcgtaa actataagcg
     1861 tggctgggat cctttttgac atgagatcga t
//
LOCUS       DAOSCC010000309         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_40683_length_1891_cov_4.090414, whole genome shotgun sequence.
ACCESSION   DAOSCC010000309 DAOSCC010000000
VERSION     DAOSCC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40683_length_1891_cov_4.090414"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PLG72_11420"
     CDS             complement(<1..116)
                     /locus_tag="PLG72_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOJ81445.1"
                     /translation="MGEKILMKGNEVIAEAALRAGCRHYFGYPITPQTEIAH"
     gene            complement(133..339)
                     /locus_tag="PLG72_11425"
     CDS             complement(133..339)
                     /locus_tag="PLG72_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ81446.1"
                     /translation="MARVTFDEDRCKGCRLCVSVCPKKIVMMDEEKLNLKGFHPATVK
                     ETEKCIGCAFCATICPDCVITVEK"
     gene            complement(486..1160)
                     /locus_tag="PLG72_11430"
     CDS             complement(486..1160)
                     /locus_tag="PLG72_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81447.1"
                     /translation="MFNNRITVFTGHFGSGKTEVAVNYAQQTARTGKKTAIIDLDIVN
                     PFFRTADVKNMLESENIKVITPVYANTNVDVPSLPAEISGMFEDKSYSVIIDVGGDDL
                     GARAVSRYRDQIANDDYIHYFVVNTRRPMTRTPEEIISMIMEIQHSAGLKVDMLVNNA
                     NVLGATTPELLAEASEILDAVSAELSIPVGFISGMEEVLSLYDGDKGIERLYMKKNIL
                     LPWERE"
     gene            complement(1282..>1891)
                     /locus_tag="PLG72_11435"
     CDS             complement(1282..>1891)
                     /locus_tag="PLG72_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOJ81448.1"
                     /translation="DDYVCITNLNRQIHAARSTVGRPKVDVMKERILDINPEAKVETY
                     QLFYLPETAGGLFDGRIDYVIDAIDTVAAKLDIVMQAKRRGIPVISSMGMANKLDPSK
                     IIVTDISKTTTDPLAKVMRKELRARGVTSLKVVFSPEVPVEPMAVNQAASEDVCTCPK
                     CRGDKPETKRRIPGSASFVPPVAGLMIAGEVIRDLAGISSCT"
BASE COUNT          414 a          505 c          417 g          555 t
ORIGIN      
        1 taatgagcta tctctgtctg aggtgttata gggtaaccga agtaatgcct gcagcccgct
       61 ctcaatgcgg cttcagcgat cacctcattg cccttcataa gtattttttc tcccatcgaa
      121 attaccccct tgtcacttct caactgttat gacgcagtca ggacaaatcg ttgcacagaa
      181 agcgcagccg atgcattttt cagtctcttt taccgttgcc gggtgaaaac ccttgagatt
      241 cagtttctct tcatccatca tgactatctt tttagggcac acagatacgc acagcctgca
      301 gcccttacaa cgatcttcat cgaaagtaac tcttgccatt tttgctatgt tgcacctcac
      361 aatctataag cctataaacg atctttcata ttataaacct cattttatat gattgcaagt
      421 aaaacagtcc ggcacggcgc cggccggaaa acctgctgtg ccccgccggt cccgaacccg
      481 cgggatcact cccgctccca gggcaacagg atattttttt tcatgtaaag ccgttcaatg
      541 cccttgtctc cgtcatacag agacaagact tcctccatgc cgctgatgaa tccgacagga
      601 atggacagtt ctgcgctgac agcatcaagt atttccgaag cttcggccag cagctccgga
      661 gtagtggcgc caaggacatt ggcgttgttt accagcatat ccaccttcag gccggcgctg
      721 tgctgtattt ccatgatcat actgatgatt tcttccgggg tccttgtcat gggccgcctg
      781 gtattgacaa caaaataatg tatgtaatca tcgttggcga tctggtccct gtatcttgaa
      841 acagcccttg ctccgaggtc gtctccgccg acatcgatta tcacgctgta gcttttgtcc
      901 tcgaacatac cgctgatctc agcgggaagc gaaggcacat ccacattcgt atttgcgtat
      961 acaggcgtga ttaccttgat attctcggat tccagcatgt tttttacatc ggctgtcctg
     1021 aaaaacgggt tgactatgtc aagatcgatt atggccgtct ttttgccggt ccgtgcggtc
     1081 tgttgggcat aattgacggc cacttcggtt tttccgctgc caaagtggcc ggtaaaaacg
     1141 gttatcctgt tgttgaacat ctgatttacc tcgtttcatt tactcttttt tatttgattg
     1201 ttcagcagta tataacgaat attgcacaca aaacatatta catctttata tctattatta
     1261 atttctgccg cttcatccaa atcaggtaca ggaactgatt cctgccaggt cgcggatcac
     1321 ttcaccggcg atcataagcc cggcaaccgg cggcacgaag gaagcgctgc ccggtatcct
     1381 ccttttggtt tcgggcttgt ctcccctgca tttggggcat gtgcacacat cttcgctggc
     1441 tgcctggttt acggccatag gctccaccgg cacttccggc gagaaaacca ctttaaggga
     1501 tgtgacgcct cttgccctga gttccttcct catcactttt gccagaggat cggtggtcgt
     1561 tttgctgatg tccgtcacaa tgatttttga gggatccagc ttgttggcca ttcccatact
     1621 gcttatgaca ggtatgcctc ttcgcttcgc ctgcatcacg atatccagtt ttgcggcgac
     1681 agtatctatg gcatcgataa cataatctat cctgccgtca aacagtccgc cggctgtttc
     1741 aggcaaataa aaaagctgat atgtctcaac cttcgcttcg gggttgatat ccaggatcct
     1801 ttctttcatg acgtcgacct tggggcggcc tacggtgctc ctggcggcgt ggatctgcct
     1861 gttgaggttt gtaatgcata catagtcatc g
//
LOCUS       DAOSCC010000310         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_43390_length_1808_cov_4.890473, whole genome shotgun sequence.
ACCESSION   DAOSCC010000310 DAOSCC010000000
VERSION     DAOSCC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43390_length_1808_cov_4.890473"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLG72_11440"
     CDS             complement(<1..223)
                     /locus_tag="PLG72_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ81449.1"
                     /translation="MNRVILHSDLNNFYASVECLYNPKIRTHPVAVCGSQSTRHGIVL
                     AKNQIAKKFGVKAGDSIWEARSKCPGLVVV"
     gene            520..>1808
                     /locus_tag="PLG72_11445"
     CDS             520..>1808
                     /locus_tag="PLG72_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ81450.1"
                     /translation="MVRTRDGNETVNSKKKKSAGRIIVTIILVLLLLPVVLSGLLYLY
                     VNVADFRYDDPEQVISNSVPMSFSERNSFDAASMTQTTLFDNADLYYVTRDYIPDLRL
                     TESVYVNAYRLALEDSAIYVQGKAYGINVPLKIDVDLRWENGGPVIGIKGVSLGSLQI
                     PLPVKAIADEYGIRLEYPFSLDNIPVLQKAADMRIEDGFLKVGFPIDKYVIEEGVAAW
                     VYVKPATIYKDEEDDFDRLLESYNNNWLADGYISEELREYAKKFQGDPEEYQKLKVRI
                     LAAGPAKSADSFFASVKSYEDIFLRFYPGITREAVEQLRKELTYEQNYNFLKSYALDI
                     DEKFGSNAITIKKGGFVDKKSGEALDMRSVYADVPGADEIFAEGTEYCAVYCTGARSQ
                     KAVGGSRYGVCTAFRFANGRCMVLFQAEKSLYYSEITP"
BASE COUNT          496 a          392 c          479 g          441 t
ORIGIN      
        1 tgaccactac aaggccgggg catttggacc tggcttccca tatggagtcg cccgccttaa
       61 cgccgaactt cttggcaatc tggtttttcg ccagcacgat gccgtgcctg gtcgactggg
      121 agccgcacac agctaccgga tgtgtgcgta ttttcggatt gtagagacat tcgacgctcg
      181 cgtaaaaatt attaagatcg ctgtgcaata tcactctgtt catacggcag ttcaccctta
      241 ccattttcaa aagagtgtcc cataccactg tcaattcacc ggttcttttc ctccaatcga
      301 acacatgttc tgccttaatt ataataccgt tcgcatcgga aagtaaagag taatgttcag
      361 ccaaattacg gcacaaaaaa atgaacatgt gcgaaatact ggatgcggca atgaagttct
      421 tatgtattac tttgcagaat tatgttatat tataggaaac catggttccg ggaggagatg
      481 catgctgtcg gatacctgaa taaagaaaag gagatcatta tggtcaggac cagagatgga
      541 aacgaaactg tgaattcaaa aaagaaaaaa tcagcgggca ggataatagt cacaataata
      601 cttgtccttt tactgctgcc ggtagtgcta tccggtctgc tgtaccttta tgtcaacgtt
      661 gcagatttca ggtatgacga cccggaacag gtgatttcaa acagtgtgcc gatgagcttt
      721 tcggagagga attcattcga tgccgccagc atgacgcaga caacactttt tgacaatgcg
      781 gatctgtatt atgtcacgag agattacata cccgatctcc gcctgacgga atccgtatat
      841 gtgaacgcat acaggcttgc attggaagac tcggccatat atgtccaggg aaaggcatac
      901 ggcatcaatg tgccgctgaa gatcgatgtg gacctgaggt gggaaaacgg cggcccggtc
      961 ataggcataa agggtgtatc gttgggaagc ctgcagatcc ctctgcctgt aaaggcgatt
     1021 gcagatgaat atggcatccg gcttgaatat ccgttcagcc tcgataatat cccggtgctg
     1081 cagaaggcgg cagacatgcg gatcgaagat gggttcctga aagtcgggtt ccccatagac
     1141 aagtatgtta tcgaagaagg agtggctgca tgggtatacg tcaagcctgc aacgatatat
     1201 aaagatgaag aggacgattt tgacaggctg ctggaaagct acaacaacaa ctggctggct
     1261 gatggctata taagtgaaga actcagggaa tatgcaaaga aattccaggg tgatcctgaa
     1321 gagtaccaga agctcaaggt caggatactg gctgccggtc ctgcgaagtc ggcggattca
     1381 ttcttcgctt ctgtcaagtc ttacgaggat atttttctgc gcttttatcc gggcataacg
     1441 agggaagcgg tggaacagct ccgcaaggaa ctcacttatg agcagaatta taacttcctt
     1501 aagagctatg ccttggacat cgacgaaaaa ttcggcagca atgccataac gataaaaaag
     1561 ggcgggttcg tggacaaaaa gagcggtgag gcactggata tgaggagcgt ttatgcggat
     1621 gtgcccggtg ctgatgagat ttttgcggaa gggacggaat actgtgccgt ttattgcacc
     1681 ggcgcccgct cgcagaaagc tgtgggaggc tcacgatacg gcgtctgtac tgccttcagg
     1741 tttgcgaacg ggcggtgcat ggtcctcttc caggcggaaa agtcgcttta ttacagtgaa
     1801 ataacgcc
//
LOCUS       DAOSCC010000311         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_1867
            NODE_50194_length_1634_cov_3.882837, whole genome shotgun sequence.
ACCESSION   DAOSCC010000311 DAOSCC010000000
VERSION     DAOSCC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863396
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:41:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,291
            CDSs (total)                      :: 2,259
            Genes (coding)                    :: 2,233
            CDSs (with protein)               :: 2,233
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 6 of 26
            Pseudo Genes (incomplete)         :: 20 of 26
            Pseudo Genes (internal stop)      :: 3 of 26
            Pseudo Genes (multiple problems)  :: 2 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50194_length_1634_cov_3.882837"
                     /isolate="AMR_MDS_1867"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /gene="argH"
                     /locus_tag="PLG72_11450"
     CDS             complement(<1..1187)
                     /gene="argH"
                     /locus_tag="PLG72_11450"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ81451.1"
                     /translation="MKLWGGRFTANTDKAVDDFHSSIRFDSRMYRQDIAGSIAHAEML
                     GKVGVISAEEAELICSTLREILSDIENGKVHFETEAEDIHMNIEKILIERIGDTGKKL
                     HTGRSRNDQVALDLRMYLKDEVRAIRSMLLELLSVLLDISEKNTDTIMPGYTHLQRAQ
                     PITLAHHMMAYFQMFRRDTGRLEDCYARMDVMPLGSGALAGTTYPLDRNFVAEKLGFT
                     AITENSLDGVSDRDFVIELASCLSITMMHLSRFCEELILWSSAEFSFIEMDDAYSTGS
                     SIMPQKKNPDVAELIRGKTGRVYGSLIALLTMMKSLPLAYNKDMQEDKEAIFDAVDTV
                     KACLPVFTKMLSTLKIKREAMYRAACGGFTNATDLADYLVKKGIPFRSAHEIVGKMVL
                     YCI"
     gene            complement(1184..>1634)
                     /locus_tag="PLG72_11455"
     CDS             complement(1184..>1634)
                     /locus_tag="PLG72_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOJ81452.1"
                     /translation="IADMVENRLVGMKSRGVYETPGGTILYAAHRELELLCLDRDTLH
                     YKDLVAQKFAELVYYGQWYTPLREALSAFVDSTQKTVTGTVRLKLYKGNVMPAGAQSP
                     YSLYSEELSTFGKDEVYNQKDAEGFINLFGLPIKVRALVMKDKEKEK"
BASE COUNT          319 a          448 c          424 g          443 t
ORIGIN      
        1 cttatgcagt acagcaccat tttgccgact atctcatggg cgctgcggaa cgggatgcct
       61 ttcttcacaa gataatccgc caggtccgtg gcattggtga aacctccgca ggctgcccgg
      121 tacatggctt ccctctttat tttcaatgtc gagagcatct tagtaaatac gggcaggcat
      181 gctttaaccg tgtccaccgc atcgaatatg gcctctttgt cctcctgcat gtccttgttg
      241 tatgcaaggg gcaacgattt catcatcgtc aggagcgcaa tgagagagcc ataaacacgc
      301 ccggtcttgc cccggatcag ttcggccacg tccgggttct tcttctgggg catgatgctg
      361 cttccggtac tgtaggcgtc atccatctcg atgaatgaga attctgcgga agaccagagt
      421 atcagctctt cgcagaatcg gctcaggtgc atcatggtta ttgaaaggca tgaagccagt
      481 tctattacga aatccctgtc actgacaccg tcgaggctgt tttcggttat agccgtgaaa
      541 ccgagctttt cagcgacgaa gttcctgtca agcgggtagg tggtccctgc aagggcgcct
      601 gagccgaggg gcatgacatc catcctggcg taacagtctt ccagccttcc tgtatcgcgc
      661 ctgaacatct ggaaatatgc catcatgtga tgggccagcg taatgggctg cgccctctgc
      721 aggtgcgtgt atcccggcat gatggtatcc gtattttttt cgctgatatc cagcagtact
      781 gacagaagct ccagcagcat cgatctgatc gctctgactt cgtccttcag gtacatgcgc
      841 aggtcgagag ccacctggtc gttgcggctc cttcctgtat gcagcttttt gccggtgtcg
      901 cctatgcgct cgatcagtat tttttctatg ttcatgtgga tatcttcggc ctcggtttca
      961 aaatgaactt tgccgttttc gatatcgctg agtatttccc tgagagtaga acagatcagt
     1021 tccgcttctt cggcggagat cacgccgacc ttgcccagca tttcggcatg tgcgatgctg
     1081 cctgcgatat cctgcctgta catcctcgaa tcgaagcgta tggacgagtg gaagtcgtcc
     1141 acggccttgt cggtattggc ggtaaacctg cctccccaga gtttcatttc tctttctcct
     1201 tatctttcat aactaaggcg cggaccttga tcggcagtcc gaacaggttg atgaatcctt
     1261 ccgcgtcttt ctggttgtat acctcatcct tgccgaaggt cgaaagttct tcactgtaca
     1321 gtgaatacgg cgactgggcg cctgcgggca tcacattgcc cttgtaaagt ttcaggcgga
     1381 ccgtaccggt cactgtcttc tgcgtgctgt ccacgaaggc tgacagcgct tctctcagcg
     1441 gagtgtacca ctggccgtag tagaccagtt cggcaaactt ctgggctacg agatccttgt
     1501 agtgcagcgt gtccctgtcc aggcagagga gttccagttc cctgtgggcg gcatagagta
     1561 tcgttccgcc gggtgtttca tatacgcccc tggacttcat cccgacaagg cggttctcaa
     1621 ccatatccgc gatg
//
